LOCUS       DATLCH010000001         7156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8197928, whole genome shotgun sequence.
ACCESSION   DATLCH010000001 DATLCH010000000
VERSION     DATLCH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8197928"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1136
                     /locus_tag="VMZ02_00005"
     CDS             42..1136
                     /locus_tag="VMZ02_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70331.1"
                     /translation="MKNLNLNEASAYSAKSPYEIYQEWEGIPVERGFIVPDLLALELG
                     DWQRSGGKAAFVNQDGAGGMCDAMVEEIPAGGQLKPQRHMYEKAVFILQGQGATTIWN
                     EGGKKHTLEWQRGSLFSTPLNAWHQHFNAQGSEPVRMVSLTDAPVIINRYRNLDYIFN
                     NNFTFSDRYEGGADAWNGAGGRYLGSDIKRGRVWESNFIADLWAFEPKDYKERGGDNR
                     TTLFEFVDNTMSAHLSEFPVGKYKKAHRHGAGAHIVMLTGSGYSYLWPEGEYGSKRRV
                     EWGRMSMFTPPMQWWHQHFNPGAEPARYLALKPWGFKFKVEDLKDTGEDVKNGGAQIE
                     YQDQNPEVHQIFLSECKKRGAEPRMPMFDK"
     gene            1268..1654
                     /locus_tag="VMZ02_00010"
     CDS             1268..1654
                     /locus_tag="VMZ02_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUR70332.1"
                     /translation="MPLNRNSAGSAPKSSAGFTLLELLIVIAVLGILAAIAIQNVALY
                     RARSVDASMRSDLRNAAMAMESYYGEFLAYPSSDTALLLNGYRKTTGVTLTINLTTPS
                     TYTLNAARPSGSQASFTFDSSTGLIN"
     gene            complement(1749..2480)
                     /locus_tag="VMZ02_00015"
     CDS             complement(1749..2480)
                     /locus_tag="VMZ02_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR70333.1"
                     /translation="MAKTILITGGAGGICSEISRGLAADGLNVVVADYAKDAAEKVAA
                     EIRDAKGSAIAVQVDVGDPQSVASMMQQAISKYGQVDYIFCGAGVMDRVPVVEMPEEM
                     WDRLMRINLKGVFLCAQAAARHMIPRNEGRILSIASGRGVAGQARSAHYAASKAGVIA
                     FTKSLAMELAPNNITVNCVCPGATDTPMSRIGFTDDAFKKREEIPPLMDGLTHKFEIV
                     GLVRYMLSDAAKYITGQTYFLRTAK"
     gene            complement(2820..3728)
                     /locus_tag="VMZ02_00020"
     CDS             complement(2820..3728)
                     /locus_tag="VMZ02_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263046608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HUR70334.1"
                     /translation="MRICSLLPGATEIAFALGLEDQIVGVTHECDYPAEAQYKPVVVR
                     SVIDSKRMSGTEIDRKIAELLQAGKGLYFIDEAAFADAAPDVILTQGLCEVCALDYND
                     VVKAAGKMLRPPTIVSLNTHSLAELLDDIVRVGAATEQSSAAAALVGDLRTRIAQVGR
                     REPDRRPRVVCLEWFEPLYTAGHWVPEMVALAGGHDVLGRAGEPSTKIQWQDVIDAKP
                     EVLLLMPCGFDVRRTVKESTPLRALPGWNDLPAVRSENVFALNGNAYFSRPGPRLVNG
                     LEILARIIHPNTVNWSVEPTDASRLN"
     gene            complement(3801..4307)
                     /locus_tag="VMZ02_00025"
     CDS             complement(3801..4307)
                     /locus_tag="VMZ02_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70335.1"
                     /translation="MRNLLSVAVAVSLSCIFVGYGFAAQSSISMQTAERWDDIATLRL
                     EMARDHETQAGNVFQEKTANSLDTGDLLDRAGDEKFLAAENYLIASQQWDKAARAYFA
                     AGAATEARKARENIEFTQASAKRSLSDGIVFHMKAKEQFELSKNLGKKTIALDKVARN
                     LERLMEMK"
     gene            complement(4915..6243)
                     /locus_tag="VMZ02_00030"
     CDS             complement(4915..6243)
                     /locus_tag="VMZ02_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70336.1"
                     /translation="MPNIDNRRTLYPALCLGILTAAFIIAKTGRDALFFQGKGIFQLP
                     VATMMITVASLPLAILFVKAMKSFGARRARVALMIFAAAVLAFTAPFLDPGESTLLFN
                     IFIFIPSVFGVMFASLWLLASDIFDTIPKQQSAAAFGKIGASSLAGGMAGGFIAKTVG
                     PVVEPRWLIFIGALMILVVVGLVMHVHRRFPTSIVPKIPEDGKASRYLTPLAKPYALT
                     LLLIAMTGALAGLLIDVQFYISATGAAMGSKGNANYFANFYIMLNFGSLLLQLFVTPR
                     IQSKIGVGGGLMILPFALVGGAGFASAAATAFSLAVLRVTEGGLRSSVHRSIWEQAFI
                     PVEASERSLVKIAVDGIGARIAEGVAAAGLYFWVQRVAPGGNVSAPLDTRWMAWLTLA
                     TVVVWLFVTQRLRAQVRQTASAARPFGPELDCVRFPDQCPCTTELGKGIA"
     gene            complement(6468..>7156)
                     /locus_tag="VMZ02_00035"
     CDS             complement(6468..>7156)
                     /locus_tag="VMZ02_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70337.1"
                     /translation="DPELIDKLPGFKRRLQWFVENRPELSEHIETESTNAGPRRFLSL
                     VNRHRLKRVLHYMLDENEFLSSYGIRALSRYHADHPYKLGVNGTEYRVDYEPAESTTG
                     MFGGNSNWRGPIWFPVNFLLIESLQKFHYFLGDNYKVECPTGSGQTANLWEVAGEISQ
                     RLTRIFSRGADGRRPVFGGTEKFQNDPHWRDLIPFHEYFHGDNGAGIGASHQTGWTAL
                     VAKLIQQYGE"
BASE COUNT         1622 a         1950 c         2014 g         1570 t
ORIGIN      
        1 tcagttaatt tcagccggtg aagatttaag gagaaccaac gatgaagaac ctgaatttga
       61 acgaagcttc ggcttattcg gcgaagagtc cctacgaaat ttatcaggag tgggaaggaa
      121 ttccagtgga aaggggattc atcgtgccgg atttattggc acttgagttg ggcgattggc
      181 agcgcagcgg cggcaaggca gcgttcgtca atcaggacgg cgccggtggt atgtgcgacg
      241 ctatggtcga agagattcct gccggcggcc aactcaaacc gcagcggcat atgtatgaaa
      301 aggcggtttt cattctgcag ggccagggcg caacgacgat ttggaacgaa ggcggcaaaa
      361 agcacacgtt ggagtggcag cgcggcagtc tcttttcgac gccgctcaat gcctggcatc
      421 aacacttcaa tgcccagggc agcgagccgg tgcgaatggt ttcgctcacc gatgctccgg
      481 tcatcatcaa tcgttaccgc aatctcgact atatttttaa caacaacttc acttttagcg
      541 atcgctacga aggtggcgcc gatgcgtgga acggcgccgg cggacgttac ctgggttcgg
      601 acatcaagcg cggccgtgtt tgggagtcca actttatcgc cgacctgtgg gcattcgaac
      661 cgaaggacta caaagagcgc ggcggcgata accgcaccac tcttttcgag tttgtcgaca
      721 acacgatgag cgcgcatctg tcggaatttc ctgtcggcaa atacaagaaa gcgcatcgcc
      781 atggcgccgg cgcgcatatc gtcatgttaa ccggatcagg ctactcgtat ctttggccgg
      841 aaggcgagta cggcagcaag cggcgtgtcg agtggggccg gatgagcatg ttcaccccgc
      901 ccatgcagtg gtggcaccag cattttaatc ctggcgccga gccggcgcgg tatctagctc
      961 tcaagccttg gggttttaaa ttcaaagtcg aagacctcaa ggataccggc gaggacgtta
     1021 agaacggcgg agcgcagatt gaatatcaag accagaatcc cgaggttcat caaatatttc
     1081 tcagcgaatg caaaaaacgc ggcgccgaac cgcgcatgcc gatgttcgat aagtaggctg
     1141 cgcggcgtta tttttgctga ggggaaaaag ccggtccagc cgcgtcaggc tctccgaatc
     1201 ggcgtttttt ctcgacaggg cattcccgtc atggtaaaaa taacaaagtt gacagccact
     1261 cgtcgtaatg ccgctcaata gaaactccgc cggatcggca ccaaaaagca gcgcgggctt
     1321 caccctgctg gaactcctca tcgttattgc ggtgctgggg atcttggccg ccatcgcgat
     1381 tcagaatgtt gctctttatc gggcgcgcag cgtcgatgcc tccatgcgat ccgatctgcg
     1441 caacgcggca atggcgatgg aaagctatta cggagagttt ctcgcgtatc cgagcagtga
     1501 cacggctctt ttattgaacg ggtaccgaaa aacaactgga gtcacgctga caatcaatct
     1561 gacgacgccc tcgacctaca cattaaatgc ggcacgaccc tccgggagtc aggcaagttt
     1621 cactttcgat agcagcacgg ggttgatcaa ctgaccgcct agcgcctggt ttgtgacctt
     1681 ttcccagcga tttccgagac aaaaatagcc gcgcagccat ggctgcgcgg ctatttttat
     1741 ttgtaaggct acttcgcggt gcgcagaaaa taggtttgac cggttatata tttcgccgcg
     1801 tcggagagca tgtaacgcac cagaccaacg atttcgaact tatgcgtgag gccgtccatt
     1861 aacggcggga tttcctcgcg cttcttgaaa gcgtcgtcag tgaagccgat gcgcgacatc
     1921 ggtgtatcgg ttgcgcccgg gcaaacgcag ttgactgtta tgttgttcgg cgccaactcc
     1981 atggcgaggg attttgtgaa agcaatcacg ccggctttgg atgccgcgta gtgggctgag
     2041 cgcgcttgcc ccgcaacgcc gcggccggat gcgatactta gaatccgtcc ttcgttgcgc
     2101 ggaatcatgt gacgcgcggc agcttgggcg cagagaaaaa caccttttaa gttaatccgc
     2161 atcaggcggt cccacatttc ttccggcatc tcgacgaccg gcacgcggtc catgacgccg
     2221 gcgccgcaaa aaatataatc gacttggccg tacttcgaga tcgcctgctg catcatcgac
     2281 gcgacgcttt ggggatcgcc gacgtcgact tgcacggcaa tagcgctgcc tttagcgtcg
     2341 cgaatttcag cggcaacctt ctccgccgcg tctttggcat aatccgccac cacgacgttt
     2401 agtccatcgg ctgcgaggcc gcgggaaatc tcggaacaga ttccgccggc gccgccggta
     2461 atcagaattg tttttgccat cactggatcc ttccactcgg ttgcgaattc agcgaggcta
     2521 tcaccgcggt ttgccagcgt caacttttgc caaacctagt agtgattcag ttcgagcgga
     2581 tctctcactt cgttcgagat gacaacttga ccattcctac tgtcatttcg agcgaggcga
     2641 gaagtctaaa gctagaagtc tatccatgct gacggcgact gagacggcga aactgcacgc
     2701 cgtatcggcc ttggaaaacg acagagagaa acgtttcgat ccgcatagcg ccgttggcgc
     2761 gaggaagaat ttcccacttc gacgtctgca ctcccaatca ttggctccaa tgcacgcagt
     2821 caattcagtc gcgacgcgtc ggttggttcc accgaccaat tgaccgtgtt cggatgaatg
     2881 attcgtgcga ggatttccag cccgtttacc aggcgcgggc cggggcggct gaagtaggca
     2941 ttgccgttga gagcaaaaac attttcgctg cgcaccgcgg gtaaatcgtt ccatcccgga
     3001 agggcgcgca gcggtgtcga ttctttgacg gtacggcgga catcgaaacc gcacggcatc
     3061 aacagtagga cttccggttt tgcgtcgata acatcctgcc attggatttt cgtcgacggt
     3121 tcgccggcgc ggccgagcac gtcatggccg ccggcgaggg cgaccatttc ggggacccag
     3181 tggccagccg tgtagagcgg ttcgaaccac tccaagcaaa cgacccgcgg tcggcggtcc
     3241 ggttcgcggc ggccgacttg cgcgatgcgt gtgcgcaaat cgccgaccaa cgctgcggca
     3301 gcactgctct gttcagtggc cgcgccgacg cgcacaatgt cgtcgaggag ctctgccaag
     3361 ctatgggtat tcagagaaac gatcgttggc gggcggagca ttttgccagc ggctttgacg
     3421 acatcgttgt aatcgagcgc gcaaacttcg cacagccctt gggtaagaat gacatcgggc
     3481 gccgcgtcgg caaaggcggc ttcgtcgatg aagtacagac ctttccccgc ttgtagcagt
     3541 tcggcgattt tgcggtcgat ctccgtaccg ctcatgcgct tggagtcgat aacgcttcga
     3601 accacgaccg gtttatattg cgcctcggca gggtagtcgc attcatgcgt gacaccgacg
     3661 atttgatctt ccagtcccaa agcaaaagca atttcagttg cgccgggcag cagagagcag
     3721 attcgcatgc tcgatgcata gcgtatgtgc cgcctttatg ctaccggcac cggtttatgt
     3781 cacagcaata cctttgagga ttatttcatt tccatgagac gttcgagatt tctagctacc
     3841 ttgtccagtg caatggtttt ctttccgaga ttcttcgaca attcgaattg ctcttttgct
     3901 ttcatatgaa atacgatccc atcgcttaaa gatcgcttcg ccgatgcttg ggtgaactct
     3961 atattctccc gtgctttcct tgcctcggtg gcagcgcccg ccgcgaagta tgcccgagct
     4021 gccttgtccc actgttggct ggcgattaga taattttctg cggccaaaaa cttctcgtca
     4081 ccggcgcgat ctagtaagtc gccggtatcg agggaatttg ctgtcttttc ttgaaaaaca
     4141 tttccagctt gggtctcatg atccctcgcc atctccaggc gtaatgtagc gatatcgtcc
     4201 cagcgttcag cggtttgcat ggaaatagaa ctctgggcgg caaacccgta gccgacaaaa
     4261 atgcacgaaa gggataccgc aacagcgacg cttaggagat tacgcatgcg cgccttagtc
     4321 aatcgacgag agagaattga gcggcggcta ttcaaattca tagtacgcgc cgtcattttg
     4381 gatcggcaaa ataaactgtt gcggaaacat tcgttatgac taaatcacaa tagccatcgc
     4441 gcgaatttgt ttcacgtctc atagcagtta tcgcagcgag caataattag acccgcgacg
     4501 attacttact ttttggtgtt ttcgagaagt aaaaagcgag tatgagaaac aaaagtggtc
     4561 gcaatccacg agctcaaagc taagtagctg cttagacgtg aggcgatttt gtcgaagttg
     4621 acgaattcaa agatttgcgc tcttcgcgta atcgagtgga tgaattaatg gcgaattccc
     4681 tccgatggac ggtgtctccc tttcgtaaag ggaggttagg agagattttt tacggcttga
     4741 aagatcgttc cagcacagac tctgtttgtc gcaacgcggc cggtggtgtg aatctgcatt
     4801 cctaaacacc tcgacgtgac cgcccgtttc acggcgtcaa cactcgcacc agcgaaaaac
     4861 aatgctttgg cggattccga gatgtgctgg gggcggagca ggttctaaac tgcttcaggc
     4921 tatacccttg cccagttctg tcgtgcacgg gcattgatcg ggaaaacgca cgcagtcgag
     4981 ttccggcccg aatggtcgcg ccgcggatgc cgtttgccgc acttgcgcgc gcagccgctg
     5041 ggtgacgaat agccagacca ctactgtcgc gagcgtcagc caagccatcc accgcgtatc
     5101 gagcggtgcg ctgacgtttc cgcccggagc gactcgttgg acccaaaaat acaatccggc
     5161 ggcggccact ccctcggcaa tgcgcgcgcc gatgccatcg accgcgattt tgaccagtga
     5221 gcgttcgcta gcttcgaccg gtatgaacgc ttgctcccat atcgagcggt gcaccgacga
     5281 gcgcaggccg ccttcggtca cgcgcagcac cgccaggctg aaggcggttg ccgccgcgct
     5341 ggcaaagccg gcaccgccga ccaatgcgaa cggcagaatc atcaacccgc cgccgacgcc
     5401 gattttgctt tgaattctcg gtgtgacaaa gagctgcaac agcagcgatc cgaaattgag
     5461 cataatgtag aagttggcaa agtaattggc attgcccttg gagcccatcg cagcacccgt
     5521 ggccgagata tagaactgca cgtcgatgag caaccccgca agggcaccgg tcatagcgat
     5581 cagaagcagc gtcaaggcgt aaggctttgc cagcggcgtc agatatcgcg atgcttttcc
     5641 atcctcgggt atcttcggca caatgctggt cggaaatctt cggtgcacat gcatgaccaa
     5701 gcctacgacg acgagaatca tcagcgcgcc gatgaagatc aaccacctgg gttcgaccac
     5761 aggaccgacg gttttggcga taaaccctcc ggccataccg ccggcaagag aactagcgcc
     5821 gattttgccg aaagcggcgg ccgattgctg cttgggaatg gtatcgaaaa tatcgctcgc
     5881 tagcagccat aggctcgcga acatgacgcc gaacacgctt gggatgaaga taaaaatgtt
     5941 gaacagcagc gtcgactcac cgggatcaag aaacggcgcc gtgaaagcca gcaccgcagc
     6001 ggcgaaaatc atcaacgcga cacgcgcccg ccgcgcaccg aagctcttca tcgctttaac
     6061 gaacaatatc gccagcggca gcgacgctac cgtaatcatc atcgtcgcca ccggtagttg
     6121 gaaaatcccc ttgccctgaa agaacagcgc gtcgcggccg gttttagcga tgatgaaagc
     6181 tgcggttaga atgcccaggc aaagggcggg gtagagggtg cgccggttgt cgatgttggg
     6241 catttgctgt tggtagctcc acagagacgt tagaaatagg tagtcagtgg tcagtattca
     6301 gttatcagtg aaagccgccg cccggtttga attggcgacg tagatcccaa tcggcagcat
     6361 tggggtcaat gactctcaca actaagtctt tataggttcc cgccatgcat tagacatcta
     6421 ttccgatcgc cagaaactga caactgaatc caggattgcc aactagttca ttccccgtac
     6481 tgctgaatca gttttgccac cagggcggtc cagccggttt gatggctcgc gccaataccc
     6541 gcgccgttgt cgccatggaa gtattcgtgg aatgggatca gatcgcgcca gtggggatcg
     6601 ttttgaaatt tctcggtgcc gccgaaaacc ggccggcgac catcggcgcc acggctaaag
     6661 attcgtgtca ggcgctggga aatctcgccg gcgacttccc agagattggc ggtttggccg
     6721 gagccggtgg gacattcaac tttgtagttg tcgcccaaga aataatggaa cttctggaga
     6781 gattcgatga gaagaaaatt gaccggaaac cagatcggtc cgcgccagtt ggagttgccg
     6841 ccaaacatcc cagtggtcga ttccgccggc tcatagtcga cgcggtattc cgtgccgttg
     6901 acgccgagct tgtagggatg gtcggcgtga tagcgcgaca gcgcgcgaat gccataggac
     6961 gataaaaatt cgttttcgtc gagcatgtaa tgcagcacgc gtttcaagcg gtgccggttg
     7021 accagcgaca gaaagcggcg cggccctgca ttggtcgatt cggtttcgat gtgctcgctc
     7081 agttcgggac ggttctcgac gaaccattgc aagcgacgct tgaagccggg cagcttatcg
     7141 atcaactcgg gatcga
//
LOCUS       DATLCH010000002         4507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5217207, whole genome shotgun sequence.
ACCESSION   DATLCH010000002 DATLCH010000000
VERSION     DATLCH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5217207"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1013)
                     /locus_tag="VMZ02_00040"
     CDS             complement(51..1013)
                     /locus_tag="VMZ02_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HUR70338.1"
                     /translation="MPQLIQTKPKIGVSSCLLGEKVRFDGGHKRDSFLTDLFGKFVEW
                     VAVCPEAEVGMGIPRDSVRLVGSSSHPKIIAEKSGRDWTVEMEKFSAERISRLAGQQL
                     SGYVFKRSSPSCGMERVKIYDKNKQPSRSGRGLFAAAVMERLPLLPVEEEGRLNDLGL
                     RENFIERVFAYRRWQDALSGANSTGALVRFHTRHKFLLLAHSERHYRQLGRIAATAKQ
                     TSLAQAYEDYGRGFMAALAIHATAKTHTNVLDHMMGYFSPQLTTSEREELRGLIHDYR
                     RQLIPLIVPITLIRHYSNKYRVAYLEGQVYLEPSPKELMLRNHV"
     gene            1113..2021
                     /locus_tag="VMZ02_00045"
     CDS             1113..2021
                     /locus_tag="VMZ02_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HUR70339.1"
                     /translation="MKIVITGATGMIGSVLIDRLSTRLDTSLILLSRNPGREIHVANK
                     QWLLWQPGSDGEWERSIDGADAVINLAGEPIAGKRWSAQQKLILRSSRIDSTRSLVEA
                     IGKAKIRPKTLISASAVGYYGPHGDERLDETSKPGEDFLSRLCVDWENEAKKAEAFDV
                     RVCLLRTGVVLAKGAGALRKMVPPFKLFAGGPLGSGQQWMPWIHINDEVSLIEFLLDR
                     ADAHGAFNGTAPNPVTMEEFSRTLGGVLDRPSWMSVPPSILALMIGEMADMLLTGQRA
                     APEAALKMGFQFKYSHLADALSALRL"
     gene            2065..2985
                     /locus_tag="VMZ02_00050"
     CDS             2065..2985
                     /locus_tag="VMZ02_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HUR70340.1"
                     /translation="MNLSQFKSDSGTARNALAAKQAHFGAAILSGDALGAMQIVDELV
                     FTRQTLSDIYLRVIAPSLVGVGDSWCRGTATTGEEKLATQIVLDQMERLRALFITPGP
                     RSPYRVMIACVEGEHHFIGARMIADLCANRGWNVDFLGHNVPDPSLIEMVQRRRPQVL
                     ALSLTMVSGLEHLRLLANLLAETAPDVRTVVGGSAMDSQSAVALRFDARWEIAKDILS
                     GVALIGNLLRADRPKSVLKEYQLVLARRVRSLRTQRGWTQEHLAEVTQVTRACINAVE
                     GGKQNVSMDILVRMANALEVPPEKLLSAEA"
     gene            3009..4133
                     /locus_tag="VMZ02_00055"
     CDS             3009..4133
                     /locus_tag="VMZ02_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HUR70341.1"
                     /translation="MKNQGLTMYSRPTAGNRRIAPLTDDKPGRQASATGSESGLFADG
                     RLAELLGVAHSPAVENALRAALLDPVASLLANPGKRIRAQLVAMTYRLFHDERAVSLS
                     AAKQCRDCAEVVELIHAGSLIVDDIEDGSAMRRGRPAMHVQYNLPLALNAGNWLYFWP
                     FEFLKEAGLTADQLVSAYERYNRTLLRAHFGQALDLGVKMAQLPQESVTEVCLAGMTL
                     KTGALMGFASALGGTVAGMAQPVQSLLEQFGVDLGVALQMFDDLGNVIGKCDPQKRYE
                     DLTLARPSWVWACAASTSDAYVYEEFLEATAKLPDVQLLERWLGAHDLVRVTRTSARQ
                     QLGRAYERLEQGLQFAGADWSARTFSELRSLGEEIAVAYG"
     gene            4126..>4507
                     /locus_tag="VMZ02_00060"
     CDS             4126..>4507
                     /locus_tag="VMZ02_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR70342.1"
                     /translation="MDKPRVAVIGSGFGGLAAGIRLQAAGCNTVIFEKRDQPGGRAYV
                     YRDHGFTFDAGPTVITAPHCLEELFTVAGKSMRDYIQLLPVKPFYRLFWDDGYVFDYS
                     DDDDALEKQIRAKSPADWEGYQRFL"
BASE COUNT          967 a         1166 c         1335 g         1039 t
ORIGIN      
        1 agccaaagct cggttcgcag ccaaaaagtt ttcagcttca accttctctt tcagacatgg
       61 ttacgcagca tcagttcttt cgggctcggc tcgagataga cttgcccttc gaggtacgcg
      121 actcgatact tattgctgta atgacgaatc agcgtaatcg gcacgatcaa tgggatcagc
      181 tggcggcgat aatcgtggat caagccgcgc aattcttccc gctctgacgt tgttagctgt
      241 ggggaaaaat aacccatcat atgatcgagc acgttggtat gagtttttgc ggtggcgtga
      301 attgccaagg cggccatgaa gccccggccg taatcttcgt aggcttgcgc caacgatgtt
      361 tgtttcgcgg tggcggcaat acggcctagt tgccggtagt gtcgctcgct gtgggcgagc
      421 aaaagaaact tgtgcctggt gtgaaaccgc accaacgcgc cggtcgaatt agcgcctgac
      481 agagcgtcct gccaacgtcg gtaggcaaaa acccgctcga taaaattttc ccgcagtcca
      541 agatcgttca gccggccttc ttcctcgacc ggaagcagcg gcaacctttc cataacggcg
      601 gcggcaaaca atccacgccc ggatcgggag ggttgcttat tcttgtcgta gatcttgacc
      661 ctttccatgc cgcagcttgg agaactgcgc ttgaaaacgt aaccggataa ctgttgaccc
      721 gctaaccggg agattcgttc agccgaaaat ttttccatct cgacggtcca gtcgcggccg
      781 gatttttccg cgattatttt gggatgactg gacgagccga ccaaacgcac gctgtcgcgc
      841 ggaatgccca tgccgacttc cgcttcgggg caaaccgcaa cccactcgac gaacttgccg
      901 aaaagatcgg tgagaaatga gtcgcgcttg tgaccgccgt cgaagcgaac cttttcaccc
      961 agaaggcatg aactgacgcc aatcttgggc ttagtctgaa ttaattgcgg catagggatg
     1021 ttgactatgg ataacatttt ctataaaaga gtcaattata gattacctag ccgggacaat
     1081 tcaggatatt ccgggaagag aagcagaatt cagtgaagat cgtaattact ggcgctacgg
     1141 gcatgattgg ttcggtattg atcgatcgat tgtcgacgcg ccttgacacc tctctgattt
     1201 tgctcagccg caaccccgga cgggaaattc acgtggcgaa taagcagtgg ttgttgtggc
     1261 agcccggatc agatggtgag tgggagcgat ccatcgatgg cgctgatgcc gtgatcaatc
     1321 ttgccggtga gccgattgcc ggcaaacgct ggagcgctca gcaaaagcta atcctgcgct
     1381 cgagccgtat agattccact cggtcgctag tcgaagctat cggcaaagcg aagataagac
     1441 ctaaaacttt gatcagcgct tcggcggtgg gttactacgg tccgcacggc gacgagcgcc
     1501 tggatgaaac gagcaaaccg ggcgaggatt ttctttcgcg tttgtgtgtc gattgggaaa
     1561 acgaagcgaa aaaggcggag gccttcgatg tgcgtgtatg tttactgcgc accggggttg
     1621 tcctggccaa aggtgctgga gcactgcgca agatggttcc gccatttaag cttttcgccg
     1681 gcgggccttt gggttcgggc caacagtgga tgccatggat tcatatcaac gatgaagtaa
     1741 gcctaatcga atttttgctt gatcgggcgg atgcgcatgg ggcttttaac ggtactgcac
     1801 ccaacccggt gacgatggaa gaattctctc gcacgctagg tggcgtgctt gatcgtccgt
     1861 cgtggatgtc ggtgccgccc tcgatcctgg cgttgatgat cggcgaaatg gcggacatgc
     1921 tattgaccgg gcaacgagcg gcacctgagg ccgcgctcaa gatgggtttc caattcaaat
     1981 attctcatct tgctgatgcc ttgtcggcgt tgcggctata gggtcgaata tcggattacg
     2041 aatggcggtt tggagacgcg aaaattgaat ctgagccaat ttaaatctga ttctggaacg
     2101 gcgcgaaacg cattagcggc gaagcaggct cacttcggcg cggcgatatt gagcggcgat
     2161 gccctgggcg caatgcaaat cgtcgatgag ctcgttttca cacggcagac cttaagcgac
     2221 atttatctgc gagtgattgc gccgtcgttg gtgggtgtcg gcgactcgtg gtgccgtggc
     2281 acagccacca ccggcgaaga aaagcttgcg acacaaatcg tgctcgacca aatggagcgt
     2341 ttacgcgcgc tgtttatcac gcccgggccg agatcccctt atcgcgtcat gattgcttgt
     2401 gtcgagggag agcaccattt catcggtgcc cgcatgatcg cggatctttg cgccaaccgg
     2461 ggctggaatg tcgactttct cggtcacaac gttcccgacc catcgctgat cgaaatggtg
     2521 cagcgacgcc ggccccaggt attggctctg tcgctaacca tggtttctgg acttgagcac
     2581 ctgcgcctgc tcgcgaacct tttggccgag acggcaccag atgttcggac cgtcgtcggc
     2641 ggctcggcca tggacagcca aagcgcggtc gcgctcaggt ttgatgcgcg ttgggaaatt
     2701 gccaaagata ttctgtccgg cgtggcattg attggtaatt tattgcgcgc cgaccggcct
     2761 aaatcggtgc tcaaggaata tcaactcgtg ttggcccgcc gcgtgcgcag tctgcgcacg
     2821 caaagaggtt ggacccagga gcatttggcc gaagtcactc aagtaacgcg tgcttgtatt
     2881 aatgctgtcg aaggcggcaa gcagaacgtg tccatggata ttctggtgcg catggccaat
     2941 gccttggaag tgccgccaga aaaattgctg tccgcagaag cataagtttt cgagagggaa
     3001 ttgatagaat gaaaaatcaa ggcttaacga tgtatagccg tccgacagcg ggcaatagac
     3061 gaatcgcacc gttaacggat gacaagccgg gacgccaagc cagcgcgacc ggcagtgaat
     3121 cgggtttgtt tgcggatggc cggttggcgg agttgcttgg tgtcgctcac tcgccagcgg
     3181 tggaaaacgc tttgcgcgct gctttgctcg atccggtggc gtcgctgttg gccaatccgg
     3241 gcaagcgaat ccgcgcccaa cttgtggcga tgacgtatcg gctgtttcac gacgagcgcg
     3301 cggtgtctct cagcgctgcg aagcaatgtc gcgattgcgc tgaggtcgtt gaattgatcc
     3361 atgccggctc gctgattgta gatgacatcg aagacggcag cgcgatgcgc cgcggccggc
     3421 cggcgatgca cgtgcagtat aatcttcctt tagcgctcaa cgctggaaat tggctgtact
     3481 tctggccgtt cgaatttctc aaagaagcgg gactgactgc ggaccaactg gtttccgcat
     3541 atgagcggta taatcgaacg ctcttgcgcg ctcacttcgg tcaggctctc gatcttggcg
     3601 tgaaaatggc gcagctgccg caggagtcgg tgacggaagt ttgtctcgcc ggtatgacac
     3661 ttaagaccgg tgcattgatg ggttttgcat cggcattggg cggaactgtc gctggcatgg
     3721 cccaacccgt acagtcgctg cttgaacaat tcggcgtcga tcttggagtt gccttgcaaa
     3781 tgttcgacga cctggggaat gtcatcggca aatgtgaccc gcaaaaacgc tatgaagact
     3841 tgacgctggc ccggccgtcc tgggtttggg cctgcgcagc gagtaccagc gatgcttacg
     3901 tgtacgaaga gtttctcgaa gccacagcca aattgccgga cgttcaactt ttggagcgct
     3961 ggcttggtgc gcacgacctc gtgcgtgtca cccgtaccag tgcgcggcag caattgggac
     4021 gcgcttatga acggctggag cagggattgc aatttgccgg cgccgactgg tccgcgcgga
     4081 cattcagcga attgcgatca ttgggtgagg aaattgcggt ggcttatgga taagccacgt
     4141 gtcgcggtta tcggcagcgg attcggcgga ctcgccgctg gcatccgact gcaagctgct
     4201 ggctgtaaca cggtcatttt tgaaaaacgt gatcagccgg gcggccgcgc ctatgtctac
     4261 cgggatcacg gttttacatt tgacgccggt cccacagtta ttaccgcacc gcactgtctc
     4321 gaagagctct ttacggtcgc cggcaagtcg atgcgcgact atatccagtt gctgccggta
     4381 aaaccgtttt accgtctgtt ctgggatgat gggtacgtgt tcgattactc cgatgacgat
     4441 gacgcgttgg aaaaacaaat tcgcgccaaa tcgccggctg actgggaagg ctatcaacgc
     4501 ttcctaa
//
LOCUS       DATLCH010000003         9311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2983746, whole genome shotgun sequence.
ACCESSION   DATLCH010000003 DATLCH010000000
VERSION     DATLCH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2983746"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            804..4604
                     /locus_tag="VMZ02_00065"
     CDS             804..4604
                     /locus_tag="VMZ02_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HUR70343.1"
                     /translation="MSPNKPFYTKTASGRLVWLIYLLCTLAGSAAADRVLGADAARAK
                     RVLIISTGSRFSPGFSLVDQAILGALAKLPSGAIESSGENLDILGFPSERFALIFRDY
                     LSEKYAERPPDLVILVYVGNLGTAGKVLQQLFPRTPVIVAGFTEEDIPRDQFDRFVVG
                     IAQRVNPRATIEIIRRLQPDVRRIVVISGTSEVDRSALNRVKQAADSFGGKIDFEYWD
                     DRSMTELRQAVSTLPAQTAVLFSRMFRDGAGHAVISSQAGQSIAQWANAPVYVMTDTL
                     LGMGALGGSVATIDGFGKRAGELARLILTGTAPATLPFEIRTETVPVFDWRALQRWRI
                     NESRLPTNSVIRFRPASIWDQYSQYMVVALVIFLLQAALITGLVLNRLNRRRAEKELR
                     ENQELLEMATRAGGLGLWARDVTGNQVWANSVIRAQLGIRPDESIQASDLLARIHPDD
                     RAEVIAEVARAHEGNAYYEGEFRIQLSDGEQRWIFTKGDTVNVPTGHGGRRMGVSLDI
                     TERKRMEFALRESEENFRRLVETTAAVIWQADFGSWSFTYVTPQAVRLLGYPLEQWYE
                     KDFWTSHIHPDDREEAIDTCLTKSKIAQDFDFEYRMIRSSGEIVWIHDIVSCQHNGGG
                     PYQLRGLMLDITERKRSELAIRESEERFRTVANSAPVMIWMSGADKLCTFFNKGWIDF
                     TGRTLEQELGNGWTEGICPEDVEHCLAVYRNSFDARREYSMEYRLRRRDGEYCWVLGQ
                     GVPRFEADGGFLGYIGTATDLSEIKRGDERFRLAVEASSNATVMVNDQGQIVLVNQQT
                     ETLFGYSRKELIGQSVELLVPERFRANHPHERARFVAAPQARTLGLDRDLCARRKDGS
                     EILVEIGLTPIDTPEGLLVLTSIVDISARRQAEDALEKERAFLRQVIDIDPNFIFAKD
                     REGRFTLVNQAIADAYGTTVEGLIGKTDADFNSNRDEVEYFHSKDLEVIDSLQERFIA
                     EEKLTDAGGKVRWLQTVKRPIIEETGMANQVLGASTDITMRKTVEAELRQQRDELAHV
                     SRVSLMGELAASLAHELNQPLTAILSNAQAAQRFITNKDFSLDEVREILNDIVDDNNR
                     ASEIIRKIRALVRKEELTLARLDLPAVVGEVVSLVHSDAILRNVKLNYQIGGAVAAVR
                     GDRIQLQQVMLNLFLNAFDAMKECAIDDRQLTIRVDAGVGTVEVSVCDHGSGLTSAAL
                     ESIFNPFFTTKREGLGMGLSISRAIVERHGGRLWAENNNGRGSTFYVVLPEFVALEQA
                     PELS"
     gene            4601..5242
                     /locus_tag="VMZ02_00070"
     CDS             4601..5242
                     /locus_tag="VMZ02_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR70344.1"
                     /translation="MMKVFLVDDDSSVRKALTRLIRSAGYDIETFASAQEFFKSKPEA
                     AGVACLVLDVRMPGLNGLELQQALRAANVTIPIIFITGHGDIPMSVNAMKAGAVDFLP
                     KPVRQEALLPAIEQALARASRQDAEAAELIQIERRLDTLTPREREVMALVVTGMLNKQ
                     IAYELGTVEKTIKVHRSRVMEKMQVSSFAELVRLAEKVGIPEQNRSPGTSARR"
     gene            5432..5836
                     /locus_tag="VMZ02_00075"
     CDS             5432..5836
                     /locus_tag="VMZ02_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR70345.1"
                     /translation="MSNSRPLIAIVDDDGSVRRALKRLVRSLGMNAETFASGQEFLNC
                     MDAMPSFRPGCAILDVQMPGMNGLEIRERLAYGITPIPVIFITAHDEVGVRERALASG
                     AVAFLRKPFDDELLIKTLHAALSRGAPSGDKA"
     gene            6148..6639
                     /locus_tag="VMZ02_00080"
     CDS             6148..6639
                     /locus_tag="VMZ02_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70346.1"
                     /translation="MEKNNAARILAVLFVAALGTVPISARAQDKPGNNLESVHEKLKA
                     DKKSIVSKYMELTDAEAKNFWPVYEDFQKDLQKTHERLGALLKSYAADYRSKSLTDEK
                     AKKLLDEWIALEQDEVKQRSAFIPKVTKALPAKKAARYLQIENEYRMLLRYDLAATVP
                     LAQ"
     gene            6703..7275
                     /locus_tag="VMZ02_00085"
     CDS             6703..7275
                     /locus_tag="VMZ02_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HUR70347.1"
                     /translation="MRANTRNWKLNITVALALALFTLQVFGPRAAEAASAGEIDRDAR
                     SSLAKLYQNYPGAKTLGDKAVGVLVFPSIVKGGFIFAGQFGDGALRKGGKSVAYYRSL
                     AVSYGFQAGIQAYGYVLFFMDDASLQYLNNTEGFELGVGPSLVVLDEGFGKNLSTTTL
                     QKGVYAFIFDQKGLMGGMGIQGTKITKINP"
     gene            7350..7844
                     /locus_tag="VMZ02_00090"
     CDS             7350..7844
                     /locus_tag="VMZ02_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70348.1"
                     /translation="MRIHLLNKAKKVAVAIMLMGLPSMSLAAQDKPADNMQILRDKIK
                     ADKKLVVATSMELTESEAKNFWPIYDAYQKDLQKINRRIAKLVDSYAEDARKNSLTDD
                     KAKKLIDEVNGIDQAEVNLKSTFAPKLAKALPTIKVARYLQIENKIRAIVKYDLAQGV
                     PLVK"
     gene            8078..8818
                     /locus_tag="VMZ02_00095"
     CDS             8078..8818
                     /locus_tag="VMZ02_00095"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HUR70349.1"
                     /translation="MGLKQIAGSSAALLLCFVVGCSQTGIRRTALVPDTNLPRPTRIL
                     VVDFAVNDREVKEYQGIMRQQPTIKDASERERLLAVEVKDAMAQEIVDGLKPLGFIVE
                     RVGRDSKATDTDLLIDGQFITVDQGNPLRRFVIGFGNGSSAVESHVQVYQGPDARKLL
                     DFTTQSDSGRMPGAAPMLGAGAVVSGGVTAGMVVTNAAVSGVKTYNSDVARMAAGSGD
                     QVARYLSEFFAKQGWIRADQVRKARMVY"
BASE COUNT         2168 a         2424 c         2662 g         2057 t
ORIGIN      
        1 ggacttatga caaagtgcgc atataatttt acccacgaaa gcgggaccgt aggatcgcac
       61 tggacaagta atacgatact tgattaatcg gttagctggt cgcagtcaca aatggtacgg
      121 ttaccggtat gctaagtgta attcttctaa tcgtttccaa tacttttatg actgttgctt
      181 ggtatgggca tttaaagcat cgatctgcac cgttaataca agttattctc atcagttggt
      241 tcattgcact cggcgaatat tgctttcaag taccggccaa tcgaatagga tacagtagat
      301 ttactggcta ccagttaaaa atcattcagg agtgtattac tatcgcgatt ttcgtagttt
      361 tcgcatactt ctatttaaac gagaccatcc gttggaatta tgccgtctcg cttgtcttcg
      421 tgtttctagc cgtcttgttt gccttctggg gacaatttta ggaaggatac tatcacctga
      481 agttttcttc agcagcaagc ttcttccgct ctctttatcc ctcaccggcg tcaccaaacc
      541 cacgcgcttg gcttgttcgc ccgctgcttg cccttgtgcg cgtattttcg acgggctttt
      601 tcttggtcgc cttgctcttt gatcgctttg ccataccact tcctcgcagt cactcgcccg
      661 caaatcacgg ctacagataa tgtctttgaa caaattccgt caatactact cttcaccgcg
      721 gcaatctgcc aagcgtaaaa ctctatcgag catttcaaag cagttgattt ttaccgtcgc
      781 atcgccgtaa agtcagccag actgtgagtc ccaacaaacc gttttatacc aagaccgcct
      841 caggccggct cgtttggctt atttacctgt tgtgcacgct tgccggctct gccgcggcgg
      901 accgtgttct tggcgccgat gccgcgcgtg ccaaacgtgt attgatcata tccacaggca
      961 gtcgcttttc gcctggattc agcctggtcg atcaagccat actcggtgct ttggcgaaat
     1021 tgccttcggg tgcgatcgag agctctggcg agaatctcga catactgggt ttcccgagtg
     1081 agcggttcgc gctaattttt cgtgactact tgagcgaaaa atacgccgaa cggccgcccg
     1141 acctcgtcat actagtttat gtaggtaacc tgggaactgc cggcaaggtg ctgcagcagc
     1201 tttttccccg gacacccgtc attgtcgccg gtttcactga agaagacatt ccgcgcgatc
     1261 aattcgatcg ttttgttgtc ggtattgcgc agcgggtcaa cccgcgcgcg acgatcgaaa
     1321 taatacggcg tctacagccc gacgttcggc gcattgtcgt gattagcggc acgtcggagg
     1381 tggatcgcag cgctctcaat cgggtgaagc aggcggcaga ctcgttcggc ggaaagatcg
     1441 acttcgaata ttgggacgat cgcagcatga ccgaattgcg tcaagccgtg agtacactgc
     1501 cggcgcaaac ggcagttctg ttttcccgaa tgtttcgcga cggtgccggt catgcagtaa
     1561 tttcttcaca ggcgggccaa tcgatagctc agtgggcaaa cgcgcctgtc tatgtcatga
     1621 ccgatacgct tttgggtatg ggcgcgttgg gtggatcggt agcaaccatc gacggcttcg
     1681 gcaaacgtgc gggcgagctg gcgcgcttga ttttaaccgg taccgcaccg gcaacgttgc
     1741 cgttcgaaat ccgtaccgag accgtgccgg tattcgactg gcgcgccttg caacgctggc
     1801 gcatcaacga aagccgcctg ccgaccaaca gcgtcattcg cttccgacca gcctcgatat
     1861 gggatcaata cagccaatat atggtcgttg ccttggtgat tttcctattg caagccgctc
     1921 tgattaccgg cttggtcttg aatcgattaa accggcgccg ggccgaaaaa gagcttcggg
     1981 aaaaccagga attgctggag atggcgacac gcgcgggcgg gttgggttta tgggcgcgcg
     2041 atgtcaccgg caatcaagtc tgggcgaatt cggtaatccg cgcgcaatta ggtattcgcc
     2101 cggatgagtc catccaagcg agcgacttac ttgcgcgtat tcatcctgat gaccgagccg
     2161 aagtcatcgc tgaagtcgcg cgcgcgcacg agggcaacgc atactacgaa ggcgagtttc
     2221 gcattcaact tagcgatggt gaacagcgct ggatttttac caaaggagac acagtaaacg
     2281 tacctaccgg gcatggcggg cgccgaatgg gcgtttcatt agacatcacc gagcgcaaaa
     2341 ggatggaatt tgctttgcgt gagagtgagg aaaatttccg gcggttggtg gaaactacgg
     2401 ccgcggttat ctggcaggcc gatttcggga gttggtcatt tacctacgtc accccccagg
     2461 cggttcggct gctcggttat cccctcgagc agtggtacga aaaagatttc tggacatctc
     2521 atatccaccc ggacgatcgg gaggaggcga tcgacacctg tttgacgaag agcaagatcg
     2581 ctcaagactt cgatttcgag tatcgaatga tcagatcgtc gggggagatc gtctggatac
     2641 acgacattgt cagttgtcaa cataacggtg gcggtccata ccagcttcgc ggtcttatgc
     2701 tcgacatcac cgagcgcaaa cgcagcgagc tggccattcg cgagagcgag gaacgttttc
     2761 gcaccgtggc caactcagcg ccggttatga tttggatgtc gggagccgac aagctctgca
     2821 ccttttttaa caaaggctgg atcgatttca ccggtcggac gctcgagcag gagctcggta
     2881 atggctggac cgaggggatt tgtccggaag acgtggagca ctgtctcgcg gtttatagga
     2941 attccttcga cgcacggcgg gagtattcga tggaataccg cctgaggcga agagatggag
     3001 agtactgttg ggtgctcggc cagggcgtgc cgcgctttga agccgatggc ggatttctcg
     3061 gctacatcgg cacggccacc gatttgagtg agatcaagcg cggcgatgaa aggtttcggc
     3121 tggcggtcga ggcgtcctcc aacgcgaccg ttatggtcaa cgaccagggt cagatcgtgc
     3181 tggtcaatca gcaaaccgag acgctgttcg gttactcgcg caaggaacta atcggccagt
     3241 ccgtggagct gcttgtaccg gaacgtttcc gcgctaacca cccgcatgag cgtgcgcgct
     3301 tcgtcgcagc gccgcaggct cggacgttgg ggctggatcg agacctgtgc gcccggcgca
     3361 aagacggcag cgagattttg gttgaaatcg gactgacgcc gatcgacacg ccggagggtc
     3421 ttcttgtcct cacatcgatc gtcgacatca gcgcgcgcag acaggccgag gacgcgttgg
     3481 aaaaggaacg cgcttttttg cgccaagtca tcgacatcga cccgaatttt atttttgcca
     3541 aagatcgcga gggacgtttc acactggtca atcaggccat cgccgatgct tacgggacca
     3601 cggtggaagg gctgatcggc aagaccgacg ccgacttcaa ttcgaaccgc gatgaagtcg
     3661 agtacttcca ctctaaagac ctcgaggtga tcgatagcct gcaggagcgc ttcatcgccg
     3721 aagaaaaatt aaccgacgcc ggcggaaaag tgcgttggct gcaaacggtg aaacgcccga
     3781 tcattgaaga aaccggcatg gccaatcagg ttttgggcgc atcgacggat atcaccatgc
     3841 gcaaaacagt ggaagccgaa ctgcgccaac agcgcgacga gttggcgcac gtctcgcggg
     3901 tgtctctcat gggtgaatta gccgcgtctt tggcgcacga gttgaatcag ccgctcacgg
     3961 cgatcttgag taacgcccag gcggcccagc gatttattac gaacaaagat ttcagccttg
     4021 acgaggtccg cgagattctc aacgatatcg tcgacgacaa caatcgcgcc agcgaaatca
     4081 ttcggaaaat cagggcgctt gtgcgtaaag aggaactcac gttggcgcgc ttggatctac
     4141 cggcggtggt cggcgaagtc gtgtcgctcg tgcacagcga tgcgatattg cgcaacgtca
     4201 aattgaatta ccaaatcggc ggcgcggttg ctgcggtccg cggcgatcgg atacagttgc
     4261 agcaggtcat gctaaatctt ttcttgaacg cgttcgatgc tatgaaagaa tgtgccatcg
     4321 atgaccggca actaaccatc cgcgtcgacg ccggcgtcgg cacggtcgaa gtgtcggtgt
     4381 gcgatcatgg ctcgggtctg acgagcgctg cgctcgaaag tatttttaat ccgtttttca
     4441 cgaccaaaag agagggtttg ggaatgggat tatcaatcag ccgagccatc gtcgaacgcc
     4501 acggcggccg cctgtgggcc gagaataata acggtcgcgg ctccacgttt tatgtcgtcc
     4561 tgccggaatt tgtcgcgctg gagcaagcgc cagagctttc atgatgaagg tgtttttggt
     4621 cgacgacgat agttcggtgc gcaaagcgct cacgcgactc attcgttccg ccggctatga
     4681 catcgagacg ttcgcctcgg cgcaggagtt cttcaagtcc aagcctgaag ccgccggggt
     4741 cgcctgtctc gtgctcgatg tcaggatgcc cggcctcaac ggattggagc ttcagcaggc
     4801 gctgcgggcg gcgaacgtaa ccattccgat tatcttcatc accggacatg gcgatatccc
     4861 catgagcgtc aatgcaatga aagccggcgc cgtggatttt ttgcctaagc cggtgcgcca
     4921 ggaagctctg ttgccggcca tcgagcaagc gctggcacgc gcgtcgcggc aggacgccga
     4981 ggccgccgag ctaatacaga tcgagcggcg cctggacact ctcacaccgc gcgagcgcga
     5041 agtgatggct ctagtggtca cgggtatgtt gaataaacaa atcgcctacg agctcggcac
     5101 ggtcgagaag accataaagg tccatcgctc gcgcgtgatg gaaaagatgc aagtgagttc
     5161 gtttgccgag ctggtcaggc tggccgagaa agtcggtata cctgagcaaa atcgttctcc
     5221 cggcaccagt gcgcgcaggt gattatttgc cggcgtgcaa agcgccggct gctcgggttg
     5281 agccgatccg ccgttgaccg atcgtgtcgg ctaaatcctt tgttggacta aagtccaata
     5341 tacagccggt cctccctact attatcttgt cggcagccaa ggcccattga ggatgtgttt
     5401 cgtctcaagt gacctacggc aactgaaatt aatgagcaac agccgcccat tgatcgcaat
     5461 cgtcgacgat gacgggtcgg tccgtcgagc tttgaagaga cttgtccgat ccctcggcat
     5521 gaatgcggag accttcgcgt cgggacagga gtttctaaac tgtatggatg cgatgccttc
     5581 gtttcggccc ggttgcgcaa ttttggatgt gcaaatgccc ggcatgaacg gcttggaaat
     5641 acgcgagcgg ctcgcgtacg gcatcactcc cattccagta atctttatca cggcgcatga
     5701 tgaagtcggc gtgcgagagc gagcattggc ttccggagca gtggcctttc tgcgcaagcc
     5761 tttcgacgac gaactgttga ttaaaacttt gcatgcggcg ctgtcgaggg gagccccgag
     5821 cggcgataaa gcctaagccg cggcgcgctc agcattcagt tatgcatcat agcattacga
     5881 gcgaaggcga acatcgcatc atcacggtcg gcaacagcgt atggccgtga gccgatggtt
     5941 ggatgtttga caaccaaaaa aaagctgggc gcaaattgca accctccgtc gctcgcattc
     6001 agcgtcttag tccaaggcga tacgccggcg cgcgcgctgg tgtggtctat cgtaccggtg
     6061 gcgatgctcg gatatgttct cggcgccggg gcgccaggca cttaaaccgt attattaaat
     6121 agatcaatga aagggagaaa agtacaaatg gaaaaaaaca atgcggcaag aattctggcg
     6181 gtcttgttcg tggccgcgct gggcactgtg ccgattagcg cgcgggctca agataagcct
     6241 ggcaacaatc tggaaagcgt ccatgagaag ctcaaggcgg acaagaagtc gatcgtctcc
     6301 aagtacatgg agctaaccga tgccgaagcg aaaaacttct ggccggtgta cgaggacttc
     6361 caaaaggatt tgcaaaagac gcacgagcgc ctgggcgcgc ttttgaagag ctatgcggcg
     6421 gactaccgca gtaagtcgct taccgatgaa aaagccaaaa agctgctcga cgagtggatc
     6481 gccctcgagc aggacgaagt taagcaacgc agtgcgttta tccccaaggt gacgaaagcg
     6541 ctgccggcaa agaaggccgc gcgttacctg cagatcgaaa atgagtaccg catgctgctg
     6601 agatacgatc tcgcggcgac agtaccattg gcgcagtaaa aaacgcccgg cacgactgtc
     6661 gagcggcagc atttcactat tatcgatgac aggagagata gaatgagagc aaacactcga
     6721 aattggaagc taaacatcac tgtagcgctg gccttggcgc tgttcacatt acaggtcttc
     6781 ggtccaaggg cggctgaggc ggcgagcgcc ggcgagatcg accgtgatgc ccgatcgtcc
     6841 ttagccaaac tctatcaaaa ttaccccggc gccaagacat tgggtgacaa ggcggtgggc
     6901 gtgctggtat ttccgagcat cgtcaaagga ggattcatct ttgccggaca gttcggcgac
     6961 ggcgcattgc gcaaaggcgg caagagtgtc gcctattacc gttcgctggc ggtgtcgtat
     7021 ggatttcaag ccggcattca ggcctatggc tacgtgctat ttttcatgga cgacgcttcg
     7081 ctgcaatatc taaacaacac tgagggcttc gagttgggcg tcggccccag cctagtcgtg
     7141 ttggacgagg gctttggcaa aaatttatcg actaccaccc tgcaaaaagg cgtttatgca
     7201 tttatctttg atcaaaaagg actgatgggc ggcatgggca ttcagggaac caagattact
     7261 aaaataaatc cgtaggcgaa gtctcgggcc atacgccgtt caactgttaa aatcgctcaa
     7321 gcggttaagg aaactatagg agtgaagcaa tgagaataca cttattgaac aaagccaaga
     7381 aggttgcagt ggcgatcatg ctgatggggc tgccatcgat gagtttggcg gcgcaggaca
     7441 aaccggccga caacatgcag atcctgcgcg acaagattaa agcagacaag aaactggtgg
     7501 tcgcgacaag tatggaattg accgagtccg aagcaaagaa cttctggccg atctacgacg
     7561 cctatcaaaa ggatttgcaa aaaatcaatc gacggattgc caaactggtc gacagctacg
     7621 ccgaggacgc gcgcaaaaac tcgcttaccg acgacaaggc gaaaaagctc atcgacgaag
     7681 tcaatggcat cgaccaggcg gaggtcaatc tgaagagcac cttcgcgccg aagctcgcca
     7741 aggcgctgcc gaccatcaaa gtcgcgcgtt atctgcagat cgagaacaag attcgcgcca
     7801 tcgtcaagta cgatctcgcg cagggcgtgc cgctggtgaa atgactgcgg cgcggcggtc
     7861 cgaatgaccg gcactaatag aagtctcgat cagacccgcg taatatcggt acggcgagcg
     7921 tgtgccggtt ggtgacaaca agactcgagc tgggcgagcg aaaaaccgtc gggtggaatt
     7981 tttgcactat gaacttcgcc agaacagagc ggtttatcga atgagtttca tcccttcgtc
     8041 gatctcaaag agtttattca gtaagtggag gtggtgtatg gggctaaagc aaattgcggg
     8101 ttcttccgcg gcattacttt tatgcttcgt cgttgggtgt agccagacgg gtattcgccg
     8161 gacagcgctc gtgccggata ccaatctgcc gcggccgacc cgcatactgg ttgtcgactt
     8221 tgccgtgaat gatcgtgaag tcaaagaata ccagggcatc atgcgccagc agccgaccat
     8281 caaagatgct tccgagcgag agcgtttgct cgccgtcgaa gtaaaggacg cgatggccca
     8341 ggaaattgtc gatgggctaa agccgctggg atttatcgtc gaacgtgtcg gtcgcgacag
     8401 caaagccacg gataccgatc tgctaatcga cggccagttt attactgtag atcaaggcaa
     8461 cccgctgcgc cggttcgtga taggtttcgg caacggctcg tccgccgtcg aaagccatgt
     8521 gcaagtttac caagggcctg acgcgcgaaa actgctcgat ttcaccactc aatccgacag
     8581 cggcagaatg cccggcgccg cgccgatgct cggcgccggc gccgtggtca gcggcggagt
     8641 cactgccggc atggtggtca ccaatgctgc ggtctctggc gtgaagacct acaattcgga
     8701 tgttgcccgc atggccgctg gaagcggtga tcaggtcgcg cgctatcttt cagagttttt
     8761 tgccaagcag ggatggatac gcgccgatca agtgcgcaaa gcgcgaatgg tttactaaat
     8821 gggcgcacac ttggatcgag tcaacatcat tgagcaggct cccatatatt tctcggctcg
     8881 gtgtcagtta ttcctaaaaa ttcatttgta agtctttagc gaaatacccg tgagtccgcg
     8941 agtgctcgac caggtcaaag ctcttgacgc ttagtcgcac gcgacccaac tcagcgcgct
     9001 ggtataaacg tgatcgcgtc gctatggctt tcgagttcgc tgcaggtcaa atcaatcgcc
     9061 ggacatcaca ttaagatgat ttacatcctg aatgttagcc ggtgattccg atcaagttga
     9121 atgaagattg gaatctgatt agtcgagtgt gaacaactcg aggacagcgc aaatcaaaac
     9181 cagcgccagt ttaggataca aatcggccat gatcaaaatt gccagcagtt cgggattccg
     9241 caaaatgtgg aaaccgagcc gcgggtaaat cgacgcatct ttggcaaagc gtcgtgatgc
     9301 gttagagcaa t
//
LOCUS       DATLCH010000004         4964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_752492, whole genome shotgun sequence.
ACCESSION   DATLCH010000004 DATLCH010000000
VERSION     DATLCH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752492"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1483
                     /locus_tag="VMZ02_00100"
     CDS             185..1483
                     /locus_tag="VMZ02_00100"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HUR70350.1"
                     /translation="MSILIRTNPTRADYARAGLAMAIAILFATCLYAIEAPKPPDTRL
                     AALGLPTGAGADWQQWDSFLTNAVKKLAQELRPEQQEQVGDVFLDSRYQLAQLLGAGT
                     SDPVPQLFASTWDRLSPILKQSVPGMSQQSASQVTGFAAAMDGLKSLSGFGRQLGFFR
                     ITPDTLRGAARLLGTGDVDPLAYNLDLDSSLRNLLGFGGLLPEPRPSSLLEQGRLQQL
                     RNRASAAVKSLLPRPAHAAEADVDRLNEWVPETQEAKEVQDYLLEVQKMLVETSDRVL
                     AKSPLAPAQQQLYRQILFTTGWQESCWRQFVKKGAKLAPLASATGDVGIMQVNRLTWR
                     SLYDIKGLTGDIRYNGNAGAEILHHYLTRYALRKKENEQPNGNLARATYSAYNGGPGA
                     LGRYRGVRQSPTWKKVDEAFWEKFQAVSSGQELAVKSCYG"
     gene            complement(1533..2318)
                     /locus_tag="VMZ02_00105"
     CDS             complement(1533..2318)
                     /locus_tag="VMZ02_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUR70351.1"
                     /translation="MRKITKRFDWRVLLLSAAVSGFGCQAVEYTGRSQVNLLSEGDEK
                     KLGVEAYEDIIKKTPPSKNQAWQAQLKRVGQRISAAAAKPEYEWAFNVLAGKEVNAFC
                     LPGGKVAFWEGIMPVAADDTGVAVVMGHEVAHALARHGAERMSQSMGAQLLGQGIGVV
                     AGMVTPGYSEAISQAYGLGVNVAVIMPWGRSQESEADHIGLILMAKAGYDPSAAVGFW
                     ERMSKVKQGGGPPEFLSTHPSDDTRISQIKEWLPEAMKYYKKK"
     gene            complement(2696..3418)
                     /locus_tag="VMZ02_00110"
     CDS             complement(2696..3418)
                     /locus_tag="VMZ02_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR70352.1"
                     /translation="MKNGKDSERISELKNRLAESVRICVREELLENFGHVSARDPQSK
                     RIFVLRHLHERLDKVTAKDIIEVDESGRALNGKLEPPKEVFLHVAIYRKRPDVHAVVY
                     SHPLYSTILGVLGKPVLPLLANCTFIKSGIELFDVPRFIGSAEVAELMIKELDSSVAL
                     LLRGSGLVTVAENIQEATLLSILIEKSAKIQYMASAIGEPRPIVASEMPGKFGSMPTH
                     FFESTWGHYVSKSTAPAQRASA"
     gene            3715..4704
                     /locus_tag="VMZ02_00115"
     CDS             3715..4704
                     /locus_tag="VMZ02_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70353.1"
                     /translation="MKRKLFLLAAALLPSLVFANLAGAQSNKVIVGYANFSPTYAPVW
                     VAKEIGFFDKYNVNADIIFIRGGAMGTQALVSRSFDVIIAGGVAAIEAALAGAELVIV
                     AVPANRMDQAFVARKEITHPAQLKGKKIAVNSLVGSAILAIKIMLQALGINPEKEVTY
                     VTSGDAATRLAALQAGLVDATGLSQPFTLTAKRAGFNVFDSIPALESLEYPSASLIAR
                     QESVRKDPLMLDRMTRSLIEAVHFFKTRKTESKRILQKYMRTQNQDELEEAYTVLAAR
                     FIEKPYPTVQSVKTILDWSKHPKAKSVDPALFIAPQFVEKLDKEGFIAAVLKK"
BASE COUNT         1077 a         1406 c         1359 g         1122 t
ORIGIN      
        1 agaaaaaaac acccggtcgg ccgatccgat gcagcaactg tttgtcagac aaccctaagt
       61 caacggctcg tgccttactt cgtgagatgc cttcggagat cgagatttga ctttcgttta
      121 ggcgcttcct ataaggccac tcataagcgt agttttaaaa ccggctcgag gagccattta
      181 cgttatgtcc attctgatac gcaccaatcc cacgcgcgcc gactacgccc gagccggact
      241 ggcgatggcg atcgccatat tgtttgcgac ctgcttgtac gcgatcgaag cgccgaaacc
      301 gcccgacact cgcctcgcgg cgctgggctt gccaaccggt gcaggagccg attggcaaca
      361 gtgggattcg tttttgacca atgcagtgaa aaaattggca caggagttac gtccggaaca
      421 gcaggagcag gttggcgatg ttttcctcga ttcgcgttat cagctggcgc aattgctcgg
      481 cgccgggacg tccgatccgg tgccgcagtt gtttgccagt acttgggacc ggttgtcccc
      541 catcctcaaa cagtccgttc ctggaatgtc ccaacagagc gccagtcaag tcaccggttt
      601 cgcagccgcc atggatggtc ttaaatcgct gtcgggattt ggccgccagt tgggattttt
      661 ccggatcaca ccggacactc tgagaggagc ggcgcgcctg ctgggaaccg gcgacgttga
      721 cccattggcg tataacctgg acctcgattc gagtttgcgc aatttgcttg gtttcggcgg
      781 cttgctgccg gaaccgcggc cgtcatcttt gttggagcag ggacggctgc agcaattgcg
      841 caacagggca tcggcggcgg ttaaatcact gctgcctcgt ccggcccacg ccgcagaagc
      901 ggatgttgac cgtttaaacg aatgggtgcc ggaaacccaa gaggcgaaag aagtacagga
      961 ctaccttttg gaagtgcaga agatgctagt tgagactagc gacagggtgc tggccaagtc
     1021 tccgctagcg ccggcgcaac aacagctcta tcggcagatt ctttttacga ccggatggca
     1081 ggagagctgc tggcgtcagt tcgtcaaaaa aggcgcgaaa ctggcgccgc tcgcctcggc
     1141 aacgggcgac gtcggcatca tgcaagtcaa ccgcctcacc tggcgcagcc tttacgacat
     1201 caaaggattg accggagaca tccgctataa cggcaatgcc ggcgccgaga tactacacca
     1261 ctatctgact cgctatgcgc ttcgtaaaaa agaaaatgag cagcctaatg gcaatttagc
     1321 gcgggcaact tactcagcct ataacggcgg acccggtgcg ctagggcgct accgcggggt
     1381 gcgtcaaagt ccgacttgga aaaaagtcga cgaagccttc tgggaaaaat ttcaagcggt
     1441 aagctcgggg caggaattgg cggtaaaaag ctgttacggc tgagcaagat gccgcgtact
     1501 tccgcaactt aaaattttct gcgcgcagtg gtttattttt tcttgtaata cttcatggct
     1561 tcgggcagcc actccttgat ttggctgatg cgcgtgtcat cgctcgggtg ggtggacaaa
     1621 aactcgggtg ggccgccgcc ctgtttcact ttggacatac gctcccaaaa tcccacggct
     1681 gcggagggat cgtagccggc cttggccatc aagatcagac cgatgtggtc ggcctcggat
     1741 tcctgggacc ggccccaggg cataatcacc gcaacgttga ctccgagccc gtacgcctga
     1801 gagatcgcct cgctgtatcc tggagtcacc ataccggcaa ccacgccaat accctgcccc
     1861 agtagctgcg ctcccatgct ctggctcatg cgttcggcac catgacgggc caaggcatgc
     1921 gccacctcgt gccccataac gacggcgacc ccggtatcgt ctgcggcgac cggcatgatg
     1981 ccctcccaaa acgcgacctt tccgccgggt aggcaaaacg cgttgacttc cttgcccgcc
     2041 aaaacattga acgcccactc atattcgggt ttggccgccg ccgcggaaat cctttgaccc
     2101 acgcgtttca actgtgcttg ccaggcctgg tttttggacg gaggagtctt tttgataata
     2161 tcttcgtacg cttctacgcc gagttttttt tcgtcaccct cggacaacag attcacttga
     2221 gaacggccgg tatattctac ggcttggcag ccgaacccag atacggcagc gcttagcaag
     2281 aggacgcgcc agtcgaatct tttggttatc tttctcatct gcgtttcctt tcggaggtag
     2341 acttgcgccg cgacatcggc gcgcgggcac aactatccag gctgggacgc tagccgagtc
     2401 acttagtcgt gtcaaacacg aattcgggtc ggaccacgcc aagacattgg actcaatggt
     2461 ttgtctctat cacgggcagg tttttcattc ttaagctgca ttttgccacc aaaaattgcc
     2521 cggccaaatt acgcttggcg cgtggcagtg aatgcggcga gctcggactc ggaatagttt
     2581 tcatttaaaa ctttccactc cgctgcctcg gcatgcggtg cactcgtaaa gatccgggat
     2641 ttcgcttgcg gttaaaatcc gaacttcgga aaaaccgcgg agtcgatgct tccaactaag
     2701 cagatgcgcg ctgggccggc gctgttgatt tggaaacata atggccccag gtcgattcga
     2761 aaaagtgcgt cggcatgctg ccgaacttgc ccggcatttc gctcgcgaca atcggcctcg
     2821 gctcaccgat cgccgaagcc atatattgga tcttcgccga cttttcgatc aggatcgata
     2881 gcagcgtcgc ttcctgaata ttttccgcca ccgtcaccag gccgctgccg cgcagcaaca
     2941 aggccaccga agagtcgagc tccttgatca tgagctcggc gacctcggcc gagccgatga
     3001 atcgcggcac gtcgaagagc tcgatgcccg acttgataaa cgtgcaattg gccagcagcg
     3061 gcaggaccgg cttgccgagc acaccgagaa tcgtcgaata gagcggatgc gaatagacga
     3121 cggcatggac atccggcctt ttgcgataga tcgccacgtg cagaaacact tctttcggcg
     3181 gttcgagttt tccgttcagc gcgcgtcccg attcgtcgac ctcgatgatg tccttggccg
     3241 tcactttgtc gagacgttcg tgcaaatgcc ggaggacgaa aatccgtttc gattggggat
     3301 cccgggcgct gacatggccg aagttttcca acagctcctc gcggacgcaa atccgcacgc
     3361 tctcggcgag ccggtttttc agttcactga tccgctctga gtctttgcca tttttcatgc
     3421 gcgatccttt ctttcgatgc ccattgttgg tttcttgaag cgggttttca ggcttttttg
     3481 gaaatttcac cgcggagacg gcagaggacg cagagtaatt tttttaggga aatcctccga
     3541 actgtgcgaa ctctgcccct ctgcggttaa taattcttca ctcgaacctg caagcgctcg
     3601 tcgttatctt taaaaagtcg ccgcgctcca tgtactacct ttcggcgcgt cgccgcaaga
     3661 tcaggagttt acgttgacaa ttcaacagcg cttacataag catcggcaag tatgatgaag
     3721 cgcaaattat ttcttctcgc ggcggcgctt ttaccaagtt tggttttcgc taacctggcg
     3781 ggcgcgcaga gcaacaaggt gatcgtcggc tacgccaatt tttcgcccac ctatgcgccg
     3841 gtttgggtcg ccaaggagat cggctttttc gacaagtata acgtcaacgc ggatatcatc
     3901 ttcattcgcg gcggcgccat gggaactcaa gcgctggtga gccggagttt cgatgtcatc
     3961 atcgccggcg gcgtcgccgc catcgaagcg gcgcttgccg gcgccgaact cgtcatcgtc
     4021 gcggttcccg ccaatcgcat ggatcaagcg ttcgtcgctc gaaaggagat cacccacccg
     4081 gcgcaactca aaggaaaaaa gattgccgtc aacagcctgg tcggcagcgc gattctggcg
     4141 atcaagatca tgctgcaggc tttgggaatc aacccggaaa aagaagtgac ctatgtgacc
     4201 tccggcgatg cggcgacccg gctggcagcg cttcaagcgg gccttgtcga tgccaccgga
     4261 ttgtcgcagc ccttcaccct gacggccaag cgcgcgggat tcaacgtgtt cgacagtatt
     4321 cccgctctcg aaagcctgga atatccgtcg gcctcgctga tcgcccgcca agaatcggtg
     4381 cgcaaagatc cgctgatgtt ggacagaatg acgcgatcgc tcatcgaagc cgtgcatttt
     4441 ttcaaaaccc gcaagaccga atctaaacgg atcctgcaaa aatatatgcg aacgcaaaac
     4501 caggacgagt tggaagaagc ctacaccgtg ttggccgcgc gatttatcga gaagccgtat
     4561 ccgacggtgc agagcgtcaa gacgatcttg gattggtcga agcatcccaa agccaagagc
     4621 gtggatcccg ctctgttcat agcgccgcaa tttgtcgaaa agctcgacaa agaaggtttt
     4681 atcgctgcgg ttttgaaaaa gtagctggcg ctacgattac gagtccatgg caagcagaaa
     4741 tttatcgcaa ttcgcctcat gctttttttc ttctcttgct aaaacgacag ctttgtctgc
     4801 gctttcttgt tgcgttgacg caaaagcaaa ctttcaggta ggctccgccg agcatgggcg
     4861 gattgcgcgc gttactttca ccggctacgt cgttcgcagt gaaggatgat cgtgacggcc
     4921 gggttcgatc tttagggaga ttcgtcaaga tgttccacgc aaag
//
LOCUS       DATLCH010000005         6468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8203044, whole genome shotgun sequence.
ACCESSION   DATLCH010000005 DATLCH010000000
VERSION     DATLCH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8203044"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1209
                     /locus_tag="VMZ02_00120"
     CDS             136..1209
                     /locus_tag="VMZ02_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70354.1"
                     /translation="MKASIFGRISTVLFFCVLMAPLPAGAQETAGALLAKLAKLSPEA
                     RQKALVDGARAEKEVTFYTSLQPPQVEPFARVFQKRYPFVKVNGVRISGSKAVVRIQS
                     EMNAGKSLVDVINVSGEEAAALKKVGALDPYLSPQREFYPPSAKDKDGYLTAMSFLTM
                     VLGYNTNLVKRADVPKSYDDLLQPRWKGQMFLDNEAYDWFAVLLKHFGREKGLQYMRA
                     LAKQDLAMMRGRTAQAQLLVAGERAIGIVQSGNTMLDYKAKGAPVDMSILDPYLALSN
                     SIMLAKHALHPYAAALLIDWSLSEEGQSVVTSFGRVVLRKGVKQRFPELMEKELFVTD
                     SEFLGPILEESAREFRQIFLGGR"
     gene            complement(1323..1748)
                     /locus_tag="VMZ02_00125"
     CDS             complement(1323..1748)
                     /locus_tag="VMZ02_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70355.1"
                     /translation="MTTHFSGESIVDYKKIFQPDRLVEAQYLAAHRRSTRMQPELRLM
                     EAILEDAVATLMTDLRRCSGRQRRDFAEALNWINGRTDQASVFSFINICETLAIDADY
                     LRQGLIRKSAENRNLMAPGAPAFRKYPTPRRKTIRLRAV"
     gene            1871..2770
                     /gene="nhaR"
                     /locus_tag="VMZ02_00130"
     CDS             1871..2770
                     /gene="nhaR"
                     /locus_tag="VMZ02_00130"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HUR70356.1"
                     /translation="MEWLNYHHLLYFWTFARVGSVVLASEELQLTQATVSAQLKSLER
                     SLGEKLFRKAGRNLVLTETGKVVYRYADEIFSLGKEMMGTLKGRSDGRLARLTVGVTD
                     VLPKFVAYQLIEPALKLAESYRIVCREGTNEELIPALALHEIDVVLTDAPLSPSLNVK
                     AFNHLLGECGMTLFGSANLAQSLRRHFPKSLDSAPFLLPTRNTTARHSLDQWFEGQNI
                     RPRIVAEFEDSALLMVFGRHGLGCFFAPSVIAGDVRQQYGVKEIGRVDSLRERFYAIS
                     LDRKLKHPAVVAISQAAKLKLGD"
     gene            complement(2799..3281)
                     /locus_tag="VMZ02_00135"
     CDS             complement(2799..3281)
                     /locus_tag="VMZ02_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HUR70357.1"
                     /translation="MVTSMKTPASRLDLLDQSITAYMARYGITLLRMSVGVVFLWFGF
                     LKFFPGLSPAEGLATKTIEVFSFGMVRPRVALIILAAWECAVGLGLILAIFLRTTLLL
                     LWLQMAGTIAPMLLFPQEVFSHFPYAPTLEGQYIIKNLVLASAGVVVGATVRGGRLRS
                     "
     gene            3466..4500
                     /locus_tag="VMZ02_00140"
     CDS             3466..4500
                     /locus_tag="VMZ02_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70358.1"
                     /translation="MEVKSIKETHTELMMQSVKFAKDEQGRRKGQAILFKWPEIEKQL
                     GEHKHIYVVDARLGSNNHTHRLWYDVIPPHTEEGQEWKSLGHRHTVEAIIYFLKGKGH
                     SIIDGVRYDWGPGDLLSVPMFSWHRHVNDGDEPVLRIASTTGPLSLGLGQAVYEDERY
                     PEFWIYAQEGEDARKTLIPGGGGSTEAAKIGNNRAAEMYAEQVAFAMHEEQLRRNSKV
                     LVKQKDLVFEPTAMGNLAYVVDPRIGFHAKALGVVMAEIPPGKRSGAHRHLYDEINLV
                     VGGKGKIIVEDKEYNFDTLDVLSIPVFQWHQYFNTGTEPLRILGINTRLAMDNLGLSL
                     THQGEHADYV"
     gene            4597..5748
                     /locus_tag="VMZ02_00145"
     CDS             4597..5748
                     /locus_tag="VMZ02_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70359.1"
                     /translation="MEVRFGLFSCDSHAQLDRDAWTKRMSAQKWGERIPQIVEVEEDG
                     KKIDRWMVNGKVRSEWVCNCPAAMEGGVARGYYPRRWGEVPKIVYDPAERLKALDEDR
                     VDGEVLYPNGPVSNFAFLQADAAFELACVQAYNDALAEWREVSDRYVPVAIIPYMSEI
                     DVVVAEVERSVRRGHRGVVMLAEPAFTKKGLKRMNDPFWEPLWACCADLGVPMHWHGS
                     SGLVEQLSLPKWSGFSKREFHTVSTSRLCATPAQLIPNLIFSGILDRHPRLNWACAET
                     GFGWVSYVLEASDHEWETRQLWKEGVKNRPSEVFRRQIYVDFWYEKAGIELREWVGVD
                     NIMWESDYPHIAATYPRSWNFVEESLVGVPEGERKKMCYGNALRLYGLA"
     gene            5898..>6468
                     /locus_tag="VMZ02_00150"
     CDS             5898..>6468
                     /locus_tag="VMZ02_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70360.1"
                     /translation="MTNTFISVDEHVQEHPEVWTKRLSQAKWGERIPHVAKNGDGKER
                     WLIDGREIALDGVADCGAMMAQRAINPHCWSDVPAAVYDPAERLKTLDAAGIEYAVLY
                     PTVAGVAGQNFGRIDDAELELACVQAYNDWLLEEWSSFSPRFVPQCLVPLSPIDSTVH
                     EIRRCVARGHRGVIYPSIPMELRDLPHIND"
BASE COUNT         1422 a         1712 c         1915 g         1419 t
ORIGIN      
        1 cgcgcgcggc agcgccatgt ttttcaaggc gactcatgcc agcacgaacg gcgccttctc
       61 gtacttaatc gactgccgct ggtagcagcg gccaaattat gaataaccgc tttgacgatc
      121 ccaaggagaa taatagtgaa agcatcgatc ttcggtagaa tttcgacggt tcttttcttt
      181 tgtgttctca tggcaccgtt gccggcgggt gcgcaggaaa ccgcaggggc gctgctggcg
      241 aagctcgcca agctttcacc cgaagcgcgg cagaaggcgc tcgtcgacgg cgcgcgcgcg
      301 gaaaaagaag ttactttcta tacctcgctg cagccgccgc aagtcgaacc gtttgcgcgg
      361 gtgtttcaga aacgctaccc gtttgtcaaa gtcaacggcg tgcgtatttc cggtagcaag
      421 gcggtggtgc ggattcagtc cgagatgaat gccggcaagt cgcttgtcga cgtgattaat
      481 gtttccggtg aagaggcggc agcactaaag aaagtcggcg ctctcgatcc gtacttgtcg
      541 ccgcaacgcg aattctatcc accgtcggcc aaagacaagg acgggtatct tacggcgatg
      601 tcttttctga cgatggtttt gggctacaac accaacttgg ttaagcgcgc cgacgtgccg
      661 aagagttacg atgatctgct gcagcccagg tggaaaggac agatgtttct cgacaacgag
      721 gcctatgatt ggttcgcggt gctgctcaaa cattttggcc gggagaaggg gctgcagtat
      781 atgcgcgcct tggccaaaca ggatctggcg atgatgcgcg ggcgcacggc gcaagcgcaa
      841 cttctcgtcg ccggtgaacg cgccatcggc atcgtccaat ccggcaacac catgctcgat
      901 tacaaggcta aaggcgcgcc ggtcgacatg tcgatcttgg atccttatct ggcgttgtcc
      961 aattccatca tgctcgccaa acacgcgctc catccctacg ctgcggcgtt attaatcgat
     1021 tggtcgctgt ccgaggaagg gcagagcgtg gtgacgagtt tcggccgcgt ggttttgcgc
     1081 aaaggcgtca aacaacgatt tcccgaattg atggaaaaag aactcttcgt caccgactcc
     1141 gaattcctcg ggccgatctt ggaggagagc gcccgcgagt tccgccagat ctttctcggc
     1201 ggtcgctaga ctaagcgttc tcctgtgatc cgcgcgcgga tggatttcta tgttccaacg
     1261 aaaatatttg gatcttcgat gtaggttttg cgttcgtttt cagtcgtcga aaaaatttgc
     1321 cattaaactg cgcgaagtcg aatcgtctta cgcctcggtg ttgggtattt tctgaatgcc
     1381 ggtgcgccgg gcgccatgag attgcggttt tccgcactct tacgaatcag tccttgacgc
     1441 agataatccg cgtcgatcgc cagggtttcg cagatattga tgaacgaaaa taccgaagct
     1501 tggtcggtgc ggccgttgat ccagttcaaa gcctcggcga aatcgcgccg ctggcgaccc
     1561 gagcagcggc gcagatccgt catcagcgtt gccacggcat cttcgaggat cgcctccata
     1621 agccgcaact cgggctgcat tcgggtgctg cgccggtggg ccgcgagata ctgcgcctcg
     1681 accaggcggt cgggttggaa aatctttttg taatcgacaa tcgattcgcc ggaaaagtgc
     1741 gttgtcattg cggttctcct cgtcagtaga tggatctgct gtcgaatggc aacttaacca
     1801 tagttcaata gattgacaaa ttcataatgt ttgcataacc attagattat ttctaattac
     1861 cctcagaacc atggaatggc tcaactatca ccatctgctt tatttttgga cgtttgcccg
     1921 ggtcggcagc gtggtgctcg ccagtgaaga gcttcaactc acccaggcga ctgtcagcgc
     1981 gcagctcaag tcgttggaac ggtctctcgg agaaaaactg ttccgaaagg ctggacgtaa
     2041 tctggtgtta accgagaccg gtaaagtggt ctaccgttat gcggacgaga ttttctcttt
     2101 agggaaggaa atgatgggaa ccctgaaggg gcgatcggat ggccgtctgg cccggcttac
     2161 ggtcggggtc accgatgttt tgccaaaatt cgtcgcctat cagttgatcg aaccggcgct
     2221 caagctggcg gaatcctacc gaatcgtctg ccgcgaggga actaacgagg agctgatacc
     2281 ggcgctggcg ctccatgaaa tcgacgtcgt gttgaccgat gcgccgctct cgccgtcgtt
     2341 gaatgtaaag gcctttaatc atttgctcgg cgagtgcggt atgacgcttt ttggcagcgc
     2401 caatttggct cagtccttgc ggcgtcattt tccaaaatct ctcgacagcg cgccatttct
     2461 gttgcctacg cggaacacca cggcgcgtca ttcactggat caatggtttg aaggccaaaa
     2521 tatccgtccg cgcattgtcg ctgagtttga agatagtgcc ttgctcatgg tgtttggccg
     2581 ccacgggctg ggttgtttct ttgctccaag cgtgattgcc ggcgacgtcc ggcagcaata
     2641 cggcgtcaag gaaattggcc gggtcgatag tctgcgcgag agattttatg cgatctctct
     2701 agaccgcaaa ctcaagcatc ccgccgttgt ggcgatctcg caggcggcta aacttaaact
     2761 tggcgattga cacaagcgcc gcgtccaatc gccggcggtt agcttctcag acggccgccg
     2821 cgaactgttg caccgacgac aacgccggcg ctggccaaaa cgagattttt gataatgtat
     2881 tggccttcga gcgtcggcgc gtaaggaaag tggctgaata cctcctgggg aaacagcaac
     2941 atcggggcta tggttcccgc catctgcagc cacaagagca gcagtgtcgt ccgcagaaag
     3001 attgccaaga tcaaacccag accgacagcg cattcccagg ctgcaagtat gatgagagca
     3061 acgcgtggcc gcaccatgcc gaaactgaac acctctatag ttttagttgc cagcccttcc
     3121 gctggactca agccggggaa aaacttcaaa aagccgaacc agagaaacac aacgcccacg
     3181 ctcatgcgca ggagcgtaat gccgtaacgc gccatatagg cggttatgct ttgatcgagc
     3241 aagtccagac gagacgctgg agttttcatg gaagtaacca taatgttttc tccatcaagt
     3301 caagattgcc gtcgcatgca atcgcctcgc tgcaaccttg cgcgccccac ggccgttggg
     3361 cgatgcagat tcttggcata gccgaagtgt tgttatacag tccagccgaa tccgcggcgt
     3421 actgatgtcg cagattgtgc gaacaactgg agtctggagg attgcatgga agtcaaatca
     3481 attaaagaaa ctcataccga actgatgatg caaagcgtca agttcgccaa ggacgagcag
     3541 gggcggcgca aggggcaggc gattttgttc aagtggccgg aaatcgaaaa acaattgggc
     3601 gagcacaagc atatctatgt cgtcgatgcc cggctcggtt ccaacaatca cacccatcga
     3661 ctgtggtatg acgtgattcc gccgcatacc gaagagggtc aggagtggaa atcgctcggc
     3721 caccgccata cggtcgaagc gatcatttat ttcctcaagg gcaagggcca tagcatcatc
     3781 gatggcgtgc gctacgattg ggggccggga gatctgctga gcgtgccgat gttttcctgg
     3841 caccgccatg ttaacgacgg cgatgagccg gtattgcgta tcgcttcgac caccggcccg
     3901 ttgtccttgg gcttgggcca agcggtttat gaggacgaac gttatccgga attctggatc
     3961 tatgctcagg aaggcgagga tgcgcgcaag acattgatac ctggcggcgg tggcagcacg
     4021 gaagccgcga agatcggcaa caaccgagcg gcggaaatgt acgccgaaca agtcgcgttc
     4081 gccatgcacg aagagcaact gcggcgtaac agcaaagtat tggtcaagca aaaggatctc
     4141 gtcttcgaac ccacggcgat gggcaatctc gcttacgtgg tcgatccgcg catcggtttc
     4201 cacgccaaag cgctcggcgt cgtcatggcg gagatcccgc cgggcaaacg ttccggtgcg
     4261 caccgtcatc tttacgacga aatcaatctc gtggtcggcg gcaaggggaa gatcatcgtc
     4321 gaagacaagg agtataactt cgacaccctc gacgtcttgt cgattccggt attccagtgg
     4381 catcaatatt tcaacaccgg tactgagccc ttgcgcattc tcggcatcaa cactcgcttg
     4441 gcgatggaca acttgggatt gtcgctgaca catcagggcg agcatgcgga ctacgtttag
     4501 tgccatttaa tatttctcgg caggtagttt cgaataaatc gttccagtgg ttccaaaggt
     4561 gcaaagttcc attcggattt gacgagaggc tagattgtgg aagttcggtt tggtttattt
     4621 agctgcgatt cccacgcgca actcgatcgc gatgcatgga ccaagcgcat gtcggcgcag
     4681 aagtggggcg aacggatccc gcagatcgtc gaggtcgaag aggacggcaa gaagatcgac
     4741 cgctggatgg tcaatggcaa agtgcgcagc gaatgggtgt gcaactgtcc ggcagcgatg
     4801 gaaggcggcg tggcgcgcgg ctactatcca cggcgctggg gagaagtgcc gaagatcgtt
     4861 tacgatcccg ccgagcgctt gaaagcgctg gatgaggacc gggtcgacgg ggaagtgctt
     4921 tatcccaatg gtccggtgtc gaactttgca tttctccagg ccgatgccgc attcgaactt
     4981 gcctgcgtgc aggcatataa cgatgcactg gcggagtggc gcgaggtgag cgatcgctac
     5041 gtgccggtgg cgatcatccc gtacatgagc gagatcgacg tcgtcgtcgc cgaagtcgag
     5101 cgctcggtca ggcgcggcca tcgcggcgtg gtgatgttgg ccgagccggc gttcaccaaa
     5161 aaaggcttga agcgcatgaa cgatcctttc tgggaaccgc tgtgggcctg ctgcgccgac
     5221 ttgggcgtgc cgatgcattg gcacggttct tcggggttgg tcgaacaact gtcgttgccg
     5281 aagtggagcg gtttcagcaa aagagaattc cacacggtat cgacatcgcg cctgtgcgcc
     5341 acgccggcgc aattgattcc caatttaatt ttctccggca ttctcgaccg ccatccgcgg
     5401 ctcaactggg cttgcgccga gaccggcttc ggctgggtga gttacgtgct cgaagccagc
     5461 gatcacgaat gggagactcg ccagctctgg aaggaaggcg tcaagaatcg gccgagcgaa
     5521 gtgtttcgcc ggcagatcta tgtcgatttc tggtatgaaa aagccggcat cgaactgcgc
     5581 gaatgggtcg gcgtcgacaa tattatgtgg gagtctgact atccgcatat cgccgcaacc
     5641 tatccgcgct catggaactt cgtcgaagaa tccctagtcg gcgtgccgga aggcgagcgc
     5701 aagaagatgt gctacggcaa tgcgctgcgg ttgtacggcc tggcgtagtt agaggaccat
     5761 tattcttatg tcgtcatgcc ggtgaaaacc ggcatccagg agtggaggga tcgactagga
     5821 ccttgattcc ggcgtacgcc ggaatgacgg atgagaatac ccggactgtt ccgggcggaa
     5881 ataaaccgtt gcattcaatg acgaatactt tcatcagcgt cgacgaacac gttcaggaac
     5941 atcccgaggt ctggaccaag cggttgtcac aggccaaatg gggcgagcgc attcctcacg
     6001 ttgccaagaa tggcgacggc aaagagcgtt ggctgataga cgggcgggag atcgcattgg
     6061 acggtgtcgc cgattgcggc gcgatgatgg cgcaacgggc gatcaacccg cattgttgga
     6121 gcgatgtgcc ggcggcggtc tatgatcctg cggaacgttt gaagacgctc gacgcagcgg
     6181 ggatcgaata cgccgtgctc tatcccactg tagcgggtgt cgccgggcaa aatttcgggc
     6241 gcattgatga cgccgagttg gaacttgcgt gcgtgcaagc ctacaacgac tggctgctcg
     6301 aagagtggag cagcttcagc ccgcgtttcg tgccgcagtg tcttgtgccg ctttctccga
     6361 tcgactcgac cgtccatgag atccgccgct gcgttgccag gggccaccgc ggcgtgatct
     6421 atccgtcgat tccaatggag ctgcgcgatc tgccgcatat caatgacg
//
LOCUS       DATLCH010000006         5844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_14081, whole genome shotgun sequence.
ACCESSION   DATLCH010000006 DATLCH010000000
VERSION     DATLCH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14081"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..539
                     /locus_tag="VMZ02_00155"
     CDS             390..539
                     /locus_tag="VMZ02_00155"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HUR70361.1"
                     /translation="MSLILLIVLVVLLVGGLPNWGYHSFGFAPSGLAGVLLLVVIVML
                     LTGRL"
     gene            660..887
                     /locus_tag="VMZ02_00160"
     CDS             660..887
                     /locus_tag="VMZ02_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70362.1"
                     /translation="MRNFMLLLVLFIGVSQSGCMFLGGAALGALGTSGAYEVNAHSKM
                     ETIEDDYKAERISRAEYLARKSQIEKGSIVY"
     gene            896..1297
                     /locus_tag="VMZ02_00165"
     CDS             896..1297
                     /locus_tag="VMZ02_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70363.1"
                     /translation="MKVNNRVVLSFIMAGAFTTVAGCGGTNTALVGRDTLPGRATRQA
                     VQNDTLRTVERVDSTTKEIHLRASPGHPGIVTFSAETRVMYLGRVYPVNQLQSGDVIA
                     MQVEKDARGNAHTHVINLQKSSRDWAQSRND"
     gene            1310..1990
                     /locus_tag="VMZ02_00170"
     CDS             1310..1990
                     /locus_tag="VMZ02_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HUR70364.1"
                     /translation="MIQQTPEADRFKVQMSESLRHETMNSRAVTIPRHANVYTCGDSD
                     EMVYFIESGQIKLLMLSPEGKECLLAIHSRGDIFGELCLSGLGERHETAVAMKETKIK
                     QIPCDQFFARLTRDSLSEGFVRYLAVRIADQQQVIANLVTVDSEQRLGQTLLQLARSM
                     GKKDPRSIRIELKISHEELSEMVGTTRPRISLFMQRFRNLGLIETNKDHFLVIKENKL
                     TEYLAQIA"
     gene            2037..2189
                     /locus_tag="VMZ02_00175"
     CDS             2037..2189
                     /locus_tag="VMZ02_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70365.1"
                     /translation="MLWIVSVLLLAMAVFSLFATYSLIGGIVQIAVIFTLAGFAIHRI
                     WRMRAG"
     gene            2197..2451
                     /locus_tag="VMZ02_00180"
     CDS             2197..2451
                     /locus_tag="VMZ02_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70366.1"
                     /translation="MTDHRMRICVAKPLIQVGAALIFLAIVAFTYTGAGAGSREQGVQ
                     IWPVRAGIDAMKALARSPVATALVLVSGIGLVALGTRKTS"
     gene            complement(2779..3282)
                     /locus_tag="VMZ02_00185"
     CDS             complement(2779..3282)
                     /locus_tag="VMZ02_00185"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70367.1"
                     /translation="MFRNVELPESVPGRLLLHSMPGRFETIDKVWHQVQTNAVGAIVC
                     LTEDFEIRQKSMKYAEALEAGTVPCSVVPFEIREGGVPENKDAFWALANDVVRRLRSG
                     EAVLVHCAGGVGRTALLAVSVLLAMGTPIAEAENLVSRAGSMVETMPQMEMLTWCEMQ
                     AAIGSGR"
     gene            3502..5154
                     /gene="pgm"
                     /locus_tag="VMZ02_00190"
     CDS             3502..5154
                     /gene="pgm"
                     /locus_tag="VMZ02_00190"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HUR70368.1"
                     /translation="MAIHPLAGKPAPAALLIDVVALEQAYYNRKPNVDDPAQLVSFGT
                     SGHRGSSLSASLNEAHILAITQAICDYRTSRQISGPLFMGKDTHAASGPAQRTALEVL
                     AGNSVETVIQRDDGFTPTPAISRAILAHNRGKKDRLADGIVVTPSHNPPADGGFKYNP
                     PDGGPADTDVTDWIQGRANDLLRESNRSVKRISYDAALKAVTTHQEDLVGPYVNDLSS
                     VIDMESIRAQQVKIGVDPLGGAALGYWQKIAEHYRLDLTVVNAQIDPTFRFMTLDHDG
                     KIRMDCSSPFAMASLVQLKDRFQVAFGNDADADRHGIVTPTLGLMNPNHYLAVAIHYL
                     LSHRPQWSKSAAVGKTLVSSCLIDRVVESLDRKLFEVPVGFKWFSPGLFDGSVCFGGE
                     ESAGASFLCHNGSVWTTDKDGIILCLLAAEMIATTGRDAAGWFAELARAMGQPYYIRV
                     DLPATTAQKTALKALKPDALTANELAGEPITAKLTHAPGNGAAIGGLKVAAKSGWFAV
                     RPSGTEDLCKIYAESLQSEKHLLAIKNQAAAIMQDVFAKANS"
     gene            5166..>5844
                     /locus_tag="VMZ02_00195"
     CDS             5166..>5844
                     /locus_tag="VMZ02_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUR70369.1"
                     /translation="MALTIQSTIELNDGQAIPRLGLGVWMIGSGKSCESAVRTALDVG
                     YRHIDTAAFYGNETSVGQAIRDSGIAREEIYVTTKLWNSAHGDPAKALEQSLTKLKMD
                     YVNLYLIHYPVPERVRSWHVLEELRVRGKTRSIGVSNFTIRHLSELRARAETVPAVNQ
                     VELHPYLQQRELREYCRKNHIVSEAYSPLTHGERLDDPKLVAIARKYPKKQLPIDAWA
                     AGVQNNSS"
BASE COUNT         1352 a         1562 c         1648 g         1282 t
ORIGIN      
        1 cggctacgaa gacggctaca acagccgcta ccgatatggc cgcaacgata acggaaatta
       61 ctcgatcttg gataacatca tgagtcaaat cctcgctttc caaaatattc ggtagaccta
      121 atgctggcag gcctcggggg tgcccagtcc ccgaggcctg catgcggatc tccggcacac
      181 atcgctagac acagtggcgc catgcgcctc aaatccctgc ctcgaccttc tcgtcgaacc
      241 gttcgactct ttcgctgctc acgccttcgc tgcttcttct gtccatcaag aataccgagc
      301 gaaatcaccg cgcaataacc tttcacacag tggcacgtaa gttgccccta tggttattga
      361 gagcggaaag ttgaaggaag ggtacgacta tgagtttgat tctgttgatc gtattagtgg
      421 ttcttttggt cggaggtctg cctaactggg gttatcactc gtttggattc gcgccctccg
      481 ggttagccgg tgtgctgttg ttggtggtca ttgtcatgct tttgacgggc cggctctaaa
      541 cgaattgcaa ctcggcgcgt aggacacgaa ttggcgtagg gtttacttcc gaggttactg
      601 gaaatgcttc tcgtaagcct gactcagccc tactaatcgg tacaactgag gagattgtga
      661 tgagaaattt catgttattg ctggtgctct tcatcggcgt cagtcaaagc ggctgtatgt
      721 tcttgggtgg cgcggcgctg ggagccctgg gcacgagcgg cgcttacgaa gtcaacgccc
      781 atagtaaaat ggaaacgata gaagacgatt ataaagccga gcgcatttcg cgcgcggaat
      841 acctggcgcg caagtcgcag atcgaaaaag gctcgatcgt ttattaggag agataatgaa
      901 agtaaacaat cgagttgtac tgagtttcat tatggccggg gcgtttacaa cagtcgcggg
      961 ctgcggcggt actaataccg ctcttgtggg aagagatacg cttcccggca gggcaactcg
     1021 tcaagcagtt caaaatgata ccttgcggac ggtggagcgg gtcgatagta cgacgaaaga
     1081 gattcacctt cgcgccagtc cgggtcatcc tggcatagtg actttcagtg ctgagacccg
     1141 ggtgatgtat ctgggacgcg tctatccagt caaccaacta caatctgggg acgtgattgc
     1201 catgcaggtg gaaaaggatg ctcgcgggaa tgcccacacg catgtgatca atctgcaaaa
     1261 aagcagtcgg gattgggctc agagccggaa tgattgagaa tcacgaccaa tgattcagca
     1321 gacccctgaa gcggatcgct tcaaagtgca aatgtccgag tcgttgcggc acgagaccat
     1381 gaactctcgt gccgtcacga tcccgcggca cgcgaatgtt tacacctgcg gcgacagcga
     1441 cgaaatggtc tatttcattg aaagcggcca gatcaaacta ctcatgctct cgcccgaggg
     1501 caaagaatgt ctcctcgcca tccacagccg cggcgatatt tttggcgagc tatgtctgtc
     1561 gggattgggc gaacgtcacg agaccgccgt ggccatgaag gaaacaaaga taaagcaaat
     1621 accctgtgat caatttttcg cgcgcttgac tcgcgactcg ttgtcagagg ggtttgttcg
     1681 ctatctggcg gtgcgtatcg ccgatcaaca gcaggtcatt gccaatctgg tgacggtcga
     1741 cagcgagcaa cggctgggcc agacgttgtt gcagctggcc cgcagcatgg gcaaaaaaga
     1801 tccgcgcagc atccgcatcg agctgaaaat ttcccacgaa gaactatccg aaatggtcgg
     1861 cacgacgcgg ccacggatta gtttgtttat gcagagattt cgtaaccttg gactgattga
     1921 aacaaacaaa gatcattttc ttgtcattaa ggaaaataaa ctcaccgagt acttggcgca
     1981 gatcgcttaa agatgaaatc tccggcgcgt cgcaatgtcc ggaaaggatg ccagcaatgc
     2041 tgtggatagt ctcggtgctg ttgttggcaa tggccgtgtt tagtctgttt gcgacctaca
     2101 gcttgatcgg cgggattgtc cagatcgccg tgattttcac tctcgccgga ttcgccatcc
     2161 atcgcatatg gcgcatgaga gccggctgat cgcagtatga ccgaccatag aatgaggatc
     2221 tgtgtcgcta aaccgctaat acaggtcggg gccgccttga tctttctcgc catcgtcgct
     2281 ttcacctata cgggtgctgg agccggaagc cgcgaacaag gggttcagat ctggccggtt
     2341 cgagccggta tcgacgcgat gaaagctctt gcccggtcgc cggtcgcgac cgccttagtg
     2401 ttggtcagcg ggatcggcct ggttgcactc gggaccagaa aaacttcgta atgccgcgac
     2461 gccgcagttg atcgaagctg cgctgtgaga tcagtagcgc aaagtcatca cagctcagtt
     2521 agtctcgctg cgacgatggc aatcttcgcc gatatcaacg gcgcggctgt ggtcgctatc
     2581 tggtttttaa tccctgtcaa attcgtttca aatgtaaaaa gggcgtctat aacaactagc
     2641 ttgtcagatc aggacgcggc ttactcaggt aagaagagca acaacagcag tcttcgtaaa
     2701 tttatcctga gcgttaattt caacctcgac tctgttgcga aactccccaa attcaaaaag
     2761 gtctgaactc tgtcattgtt aacgacccga tcctattgcc gcctgcattt cgcaccaggt
     2821 aagcatttcc atctgtggca tggtctcgac catcgagccc gcgcgcgaga ccaggttttc
     2881 cgcctcagcg atgggtgtcc ccatggcgag cagcacggaa acggcgagta gcgcagtacg
     2941 gccgactccg ccggcgcagt gaaccagcac ggcttcgccg gagcgcaggc gacggactac
     3001 atcgttggcc agcgcccaga acgcatcttt gttttccggc acgccacctt cgcgaatttc
     3061 aaacggcacg accgaacagg gtacggttcc agcttccagt gcctcggcgt atttcatgga
     3121 tttttgacga atttcgaagt cttccgtcag gcaaacgatc gcgccgacgg cattggtttg
     3181 cacctgatgc caaactttat cgatggtttc aaagcggccg ggcatgctgt gcagcagtaa
     3241 ccgtccagga accgactcag gcaattcgac gttacgaaac atctcggtct ctccgatcag
     3301 cacttagatt cttagtcacg catacaaata tgcaatcgac actggataaa caagcggagc
     3361 ccggaagcga agtatcaact ccaaggcacg ggagattaga ctccgctgat cggcggagca
     3421 aggcgccatt ccaaatcgcc aagcttatga tagcgtttaa attgacgtgc tatgaaaaga
     3481 gtaaaaagga accgataagc gatggcgatt catccacttg ccggcaaacc ggcgccggct
     3541 gcgcttctga tcgatgtcgt tgcgctggag caggcctatt ataaccgtaa accgaatgtc
     3601 gatgatcctg cgcagcttgt gagtttcggc accagcggcc atcgcggcag ttcgcttagc
     3661 gccagtttga acgaggcgca tattttggca atcacccagg cgatctgcga ctaccgaacc
     3721 agcaggcaaa tctccgggcc gttgttcatg ggcaaagaca ctcatgccgc atccggcccg
     3781 gcgcagcgca cggcgctgga ggtgctggcg ggaaattccg tcgagaccgt cattcagcgt
     3841 gatgacggct ttactccgac cccggcgatt tcgcgggcca tcttggcgca taaccgcggc
     3901 aagaaagacc ggcttgccga tggcatcgtg gtgacgccgt cacacaaccc gcccgctgac
     3961 ggcggcttta agtacaatcc ccccgacggc ggcccggcgg ataccgatgt cacagattgg
     4021 atccaagggc gggccaatga cttgttgcgc gagagcaatc gtagcgtgaa gcgaattagc
     4081 tatgacgcgg cattgaaagc cgtgacgacg caccaagaag atttagtcgg cccctacgtc
     4141 aacgacttgt catccgtcat cgacatggaa tcgattcgag cgcaacaggt gaagatcggc
     4201 gtcgatccgc tcggtggtgc ggcgctcggc tattggcaaa aaatcgccga gcactaccgg
     4261 ctcgatctca ccgtggtgaa cgcgcaaatc gacccgacgt ttcgtttcat gacgttggat
     4321 catgacggca agattcgcat ggattgttcg agtccattcg ccatggcaag cctggtgcaa
     4381 ttgaaagatc gcttccaagt cgcttttggt aacgacgcgg atgcagatcg gcacggcatc
     4441 gtcacgccga ctcttggact gatgaatccc aatcactatc tggctgtggc gattcactat
     4501 ttgctgtcgc accggccgca atggagcaaa tccgccgccg tcggcaagac tttggtcagc
     4561 agttgtctga tcgatcgggt ggtcgaaagt ctcgatcgta agctcttcga ggtgccggtg
     4621 ggcttcaaat ggttttcgcc gggactcttc gatggctcgg tttgttttgg cggcgaagaa
     4681 agtgccgggg caagttttct ctgtcacaac ggtagcgttt ggacgacgga caaggacggg
     4741 attattctct gtctgctcgc agctgaaatg attgcgacga caggccgcga tgcggccggt
     4801 tggttcgcgg aattggcgcg tgccatgggt cagccctact acatacgcgt cgacttgcct
     4861 gcaaccacgg cgcagaagac tgctttgaaa gcgctaaagc cggatgcgct cacggccaat
     4921 gaacttgccg gcgagccgat caccgcgaag ctcacgcacg cgcccggtaa tggcgcagcc
     4981 ataggtggcc tcaaagtcgc cgccaaaagc ggctggttcg ccgtcaggcc gtcgggcaca
     5041 gaggatttat gtaagatcta cgccgagagt ttgcagtcgg agaaacattt gttggcgatc
     5101 aaaaatcaag cggcggcgat catgcaggat gttttcgcca aagcaaatag ctgacgcgct
     5161 ggcgtatggc actgacaatt caatcaacga tcgaactgaa cgacggccag gcgattccgc
     5221 ggctcgggct gggcgtgtgg atgatcggct cgggtaagag ttgcgagtcg gcagtgcgta
     5281 cagctctcga tgttggctat cgccatatcg atacggcggc gttttacggc aatgaaacga
     5341 gcgtcggtca agcgattcgc gacagcggca ttgcccggga agagatctac gtcaccacta
     5401 aactgtggaa cagcgcccac ggcgatcccg cgaaagcgct ggaacaaagt ctcactaagc
     5461 tcaagatgga ttatgtgaac ttgtatttaa tccattatcc ggtgcccgag cgagtgcgca
     5521 gctggcatgt cttggaagag ttgcgcgtcc gcggcaagac gcgttccatc ggcgtgagca
     5581 attttaccat ccgccacttg tctgaacttc gcgccagagc tgagacggtt cccgccgtca
     5641 accaggtcga gctacacccg tatctccaac agcgcgaact ccgggagtac tgccgcaaaa
     5701 accacatcgt tagcgaggct tatagtccgt tgactcacgg cgagcggctc gacgatccca
     5761 agctggtggc gatcgcgcga aaatatccga aaaagcaatt gccgatcgat gcctgggcag
     5821 cgggggttca aaacaactct tctg
//
LOCUS       DATLCH010000007         4816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1510051, whole genome shotgun sequence.
ACCESSION   DATLCH010000007 DATLCH010000000
VERSION     DATLCH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510051"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..796)
                     /locus_tag="VMZ02_00200"
     CDS             complement(149..796)
                     /locus_tag="VMZ02_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUR70370.1"
                     /translation="MNSEEFLREILAPVQEFCRLLDGNPPAYSYVPLRTDAQRIRVMQ
                     SRLFNEMRAADLFGTWLKTTPEFDVKSTMAESAHEEMVHARLLSERIHGLGGEPFNYK
                     PLPAQIAMFNAMEGLSQTCERIAAFSLAGETVANHLIQMCLKSPDVPEWIKQPYRKIT
                     QDEEGHGSVPQLFLKRYAATAELQDRVRRAVAMRMVLFREYLASLDRWAMGSDRW"
     gene            complement(842..1555)
                     /locus_tag="VMZ02_00205"
     CDS             complement(842..1555)
                     /locus_tag="VMZ02_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR70371.1"
                     /translation="MPQLRQLVIAAEEPARLANFYQEVFDLERIEENHGWLFLSDGTF
                     NLALIPEPDPAKRGFRHLGFDTARIESIRKKLSHIGMADSDVCEVNSLASIDHEFKDP
                     DGNTIGVTRRAFDVAYAARPVPIRHIALYTPNPQKLAEFYCKVLDMKEVERTDRSSIF
                     VSDGYFNLALLYQRKEEPMGLNHFGFHVQSNEEMQNRAERAGVRRGAKRPERIPFAEY
                     RVHDPEGNGIDISQKGWRV"
     gene            complement(1609..4128)
                     /locus_tag="VMZ02_00210"
     CDS             complement(1609..4128)
                     /locus_tag="VMZ02_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR70372.1"
                     /translation="MIRQSWQRWLLLFLSGVFGALLIGESIVSSGRWIGQPFPGFFVH
                     ENLTVGPYALPGWSGIAAGIQSLDHVVAVAGRRLGSRIELYDSVRSAPVNTPLHYQII
                     RDGRQLELTVPTMIFGLRDWLLSFGVYVAIGLAFLVMGVAPYIFGAATPVALPLCFMV
                     LTVFVWFQTTFDFMTEAVLPKELRIFALTLTPSAAIHLALMLSRSEAESMLRPRFVAI
                     LYGIGLALGAMNSLTFFGPLETWIHHFRAGYIFICIGALSFLWIIGRALRQSTSDLER
                     SRLRVMFVGALLGFLVPGLMTVLTSSYRLAIPYNLALVPSIFFPISIAYALLKYSLFD
                     LGNALRVTVSRIALMGLLIGVYTVVALLLAPWAGEYAKEPLIPILFSILVVAIFNPLL
                     RWLEHVVDRFVYRQDYDPVQVHEETSLFLRSLDEAPVLAQGFLDRVCKPLRIDIAVLV
                     YRPKNSGELVHAFIQKLPPDTTKLAADSDFVAGLLTSADARGMSRSEISNHPRYAEKR
                     AALLEVFGRWQAELLMPLVYERAVRGFVCFGNKWSGGEYGAEDFRLLRTLTDQLALSL
                     ENGRLYQESVEARERVEATNRKLIEMDRVKKNFVANICHELRTPVSTIIGFSEVLRDV
                     GINGGSREILDRLVNNGQELAHLMDNLMNYSRMEADSALAQVEIVKLEEILGGLEAMT
                     QRLIRQRPIKFGIHIEAEIGTIESDRQKLQQILLELVTNALKFTEKGKIELHIRRLEQ
                     RGGEILEIAIADTGIGIRLEDQEVIFEDFRQLDGSSTRQYGGTGVGLGLCKKLAGALG
                     GELHLISELGVGSVFSLRLPLKPIAESADPSKSNAVSQLLQ"
     gene            complement(4265..>4816)
                     /locus_tag="VMZ02_00215"
     CDS             complement(4265..>4816)
                     /locus_tag="VMZ02_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HUR70373.1"
                     /translation="IVTIYSNNQMNQQALHIEPGLRWYAIQTKVNRERDVEKRLTDLR
                     LEVFLPWMQARRRIGSKFHWVKVPLFPGYVFSRLDMVTSGKSARYSPGVKDFLTFGSQ
                     VAEVGDDIIAGLRERCPGGVAQVDPVKAKPGDMVRINEGPFSGLDAIFEQKLKGSERV
                     AVLLEILGRQTRVVLASETVAKI"
BASE COUNT         1191 a         1352 c         1237 g         1036 t
ORIGIN      
        1 acgaccgtat cgccgctgag cacgccgagc ttaaagtcgt cgaaaaaaag tatcttcata
       61 gttcctccga aaatgatccg atggatgcac cagtagggtc gttggcgcga gcgcaagtgg
      121 aataaaggat tagaaaatac gcagcggctt accagcgatc gctgcccatg gcccagcggt
      181 ccaagctcgc gagatactca cggaacagga ccatgcgcat ggcaacagcg cggcgcaccc
      241 ggtcttgaag ttctgctgtg gcggcatagc gttttagaaa gagctgcggc acgctgccat
      301 ggccttcttc atcttgggtg attttacgat agggctgctt tatccattcc ggaacatcgg
      361 gcgacttaag gcacatctga atcagatggt tggcgactgt ttctcccgcc agggaaaacg
      421 ccgcgatgcg ttcgcaggtt tggctcaacc cttccatagc gttgaacatc gcgatctgcg
      481 ccggcagcgg tttgtagttg aacggttcgc cgcccaaacc atgaattcgc tcgctcaaaa
      541 gccgcgcatg aaccatttct tcgtgggccg actcggccat ggtagatttc acatcgaact
      601 ccggcgtggt tttgagccaa gtaccgaaaa gatcggcggc gcgcatctcg ttgaatagtc
      661 ggctttgcat cacccgaata cgttgcgcat ccgtgcgcag cggcacgtag gagtaggccg
      721 gcggattacc atcgagcaga cgacagaact cctgcactgg tgcgagtatt tctcgcagga
      781 actcttcact attcatgatt cgactcagtc cgaagggcga acgccaacga gcaacgagat
      841 cctaaacccg ccatcccttc tgtgagatat cgatgccatt gccctcggga tcgtgcacac
      901 ggtactcggc aaaaggaatg cgctccgggc gtttggcgcc gcggcgcacg ccggctcgtt
      961 ccgcacggtt ttgcatttct tcattgctct gtacgtgaaa gccgaaatgg ttgagaccca
     1021 ttggctcctc tttgcgctgg tagagcagag cgaggttaaa atagccgtca gaaacaaata
     1081 tcgaagaacg gtcggtgcgt tcgacttctt tcatatctag cactttgcag tagaattctg
     1141 ccaatttctg aggatttggc gtgtatagcg caatgtgccg aattggcacc ggccgggcag
     1201 cgtacgcgac atcgaaggcg cggcgagtga caccgatagt gttgccgtct ggatccttga
     1261 actcgtgatc gattgaggcg agcgaattca cctcacaaac atcactatcc gccatgccga
     1321 tgtgcgagag ctttttacgg atcgattcga ttcgagctgt atcgaagccc aaatgacgaa
     1381 agccgcgctt cgctggatca ggctccggga tcaaggctaa gttaaatgtg ccgtcggaaa
     1441 gaaacagcca accgtgattt tcttcgattc gttcgagatc gaaaacttct tggtaaaagt
     1501 ttgcaaggcg tgccggttct tcggcagcta taaccaactg acgcaactgt ggcataagac
     1561 aaatcctttc gcgaagggcc taagtttcac tatgtcatgg tcgacgaatc attgcagcaa
     1621 ttggctcacc gcattcgact tcgatgggtc cgcggattcc gctataggtt ttaacggcaa
     1681 gcgcaaacta aagacactgc cgactccgag ctcacttatg agatgtaatt caccgccgag
     1741 cgctccggct aatttcttgc agagccccag cccgacaccc gtaccgccgt attgccgcgt
     1801 cgatgacccg tcgagctggc gaaagtcttc gaaaatgacc tcctggtcct ccagacgaat
     1861 acctattccg gtatcggcaa tggcaatttc caaaatttcg ccgccgcgtt gctcaagccg
     1921 gcgaatgtgc agctcgatct tgcctttctc ggtgaatttc aacgcgttcg tgacaagttc
     1981 cagcagtatc tgttgcagct tctgcctatc gctctcgatg gttccaatct ccgcttcaat
     2041 gtgaatacca aacttgatcg gccgttgacg gatcaggcgc tgggtcatgg cctcgagacc
     2101 gccaagaatc tcctccagtt tgacgatctc gacttgtgcc aaggcactat cagcttccat
     2161 gcgtgagtaa ttcatcaaat tgtccattag gtgtgccaac tcctggccgt tgttcaccag
     2221 ccggtcgaga atctcccgac tgccgccatt gatgccgacg tcgcgcagca cttcgctgaa
     2281 gccaatgatc gtactgaccg gggtacgcaa ttcatgacat atattagcaa caaagttctt
     2341 cttaacccga tccatttcaa ttagtttgcg attggtggct tcgacccgtt cacgcgcttc
     2401 taccgattct tgatagagcc gaccattttc caaggagagc gccaactgat ccgtcaatgt
     2461 gcgcaacagg cgaaaatctt ctgcgccata ttcgccgccc gaccacttgt tgccgaagca
     2521 aacgaatccc cttaccgctc tttcatagac cagcggcatc aacagttccg cttgccagcg
     2581 gccaaatacc tccaagagcg cggcgcgctt ttctgcgtag cgcggatggt tggaaatctc
     2641 gcttcggctc atgccgcgcg cgtcagcgct ggttaacagc cccgcgacaa aatcgctgtc
     2701 cgccgctagt ttggtcgtat ctggcggcaa tttttgaatg aacgcatgaa ctaactcgcc
     2761 ggagtttttg ggtcgataaa ccagcacggc gatatctata cgtagaggtt tacacacgcg
     2821 atcgagaaaa ccctgcgcca ataccggcgc ttcgtccaaa ctacgaagaa agaggctggt
     2881 ttcttcgtgc acctgcaccg gatcgtaatc ctgccgatag acgaagcggt cgaccacatg
     2941 ctcgagccag cgcaataagg gattaaaaat cgcaaccacc agaattgaaa agaggatcgg
     3001 aatcaatggc tccttggcat actcgccggc ccagggtgcc agcaacagag caaccacggt
     3061 atagactccg atcagcaggc ccattagagc aatgcggctc accgtaactc gcaaggcatt
     3121 gccgagatcg aagagactat acttgagcaa cgcgtaggcg atcgagatcg ggaaaaaaat
     3181 cgaaggtacc aacgcaaggt tgtacggaat agcgagccgg tacgagctcg tcaatacggt
     3241 catcagtccg ggcacgagga aaccaagcag cgctccgaca aacattacac gcaaacgcga
     3301 gcgttcgaga tccgaagtac tttgccgtag agcgcgccca atgatccaaa gaaaactcag
     3361 cgcaccgatg cagataaaaa tatagccggc acgaaaatga tgaatccacg tctccagggg
     3421 tccgaagaac gtcaaactgt tcatcgctcc taaagccaaa ccaatgccgt aaagaatcgc
     3481 cacgaaccgc ggccgcagca tcgattctgc ctcggagcga ctcaacatca atgctaggtg
     3541 aatcgctgcg ctaggcgtca acgtgagggc aaatatacgt aactcctttg gcaataccgc
     3601 ttcggtcatg aaatcgaagg tagtctgaaa ccagacgaac acggtcaaca ccatgaagca
     3661 cagcggcaag gcgacgggcg tcgccgcacc aaagatgtag ggcgcgactc ccatcactaa
     3721 aaacgccaaa ccgatagcga catagacacc aaaactaaga agccaatcgc gcaggccaaa
     3781 tatcatcgtc ggcaccgtca attcgagttg gcgaccgtca cggatgatct gatagtgcag
     3841 cggagtattt accggcgcgc ttcgcacgga atcgtatagc tcaatgcgac tgccgagtcg
     3901 gcggccggcg acggcaacaa catggtccag agactgaatt cccgcagcga tgccagacca
     3961 tcctggtagc gcataaggcc caactgttaa gttttcatga acgaaaaagc ccgggaaagg
     4021 ctggccgatc caccggcctg acgagacaat cgactcaccg atcagtagag caccgaatac
     4081 accgctaaga aaaagcagta gccatctttg ccagctctgg cgaatcatcc ccacccccag
     4141 cttcaaactg cgactgtaag gaaacttcga tctatcaggg gggatcgctg actgtcaagg
     4201 aagtgcgccg gcgtgatact gtcgtgcgga atacgatggc ctgcgggcga cagcgccgag
     4261 ttttctaaat cttggcgacc gtttcactag ccagtactac gcgagtctgg cgcccgagaa
     4321 tttcgagcaa gaccgcgacc cgctcactgc ctttgagttt ttgctcaaat atcgcgtcca
     4381 atccggaaaa cgggccttcg ttaattcgca ccatgtcacc gggtttagct ttgactggat
     4441 cgacctgcgc aacaccaccg ggacagcgct cacgcaatcc cgcgattata tcgtcgccta
     4501 cttccgcgac ttgactgcca aatgtaagaa aatccttaac accgggtgag taccgcgccg
     4561 acttgcccga tgtgaccatg tccagccggc tgaatacata tccgggaaag agtggcacct
     4621 taacccagtg gaacttactg ccaatacgtc ggcgcgcctg catccacggc agaaacactt
     4681 caagtcgtaa gtcggtcagg cgcttctcga catcgcgttc gcggttgact ttggtctgta
     4741 tagcgtacca tcgcagaccg ggttcgatat gtaatgcttg ttggttcatt tggttattcg
     4801 aataaatcgt gacgat
//
LOCUS       DATLCH010000008         7838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2258280, whole genome shotgun sequence.
ACCESSION   DATLCH010000008 DATLCH010000000
VERSION     DATLCH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258280"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VMZ02_00220"
     CDS             <1..457
                     /locus_tag="VMZ02_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70374.1"
                     /translation="TVFGINGFLPIHTAGNRGEQALSSLAEVIKMHHGVSHLDVGSFG
                     GGTPLIALGIEDAEIMARDSITKRQVKDYLWEHASLKFSEIPARRKGHKSDDELMRES
                     INMTPDGVVHLSTKPEDIMVIVVGGKHRHSVFLPMWTGRNTLCAIKGIK"
     gene            466..1494
                     /locus_tag="VMZ02_00225"
     CDS             466..1494
                     /locus_tag="VMZ02_00225"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70375.1"
                     /translation="MEWGVMGGRPRIDDGGLRIARTLAMVAVALLRVVPCTAQDKVRF
                     PIGASSKTFSYGPLWLGQKLGFFEREGLDVQIVVMRGTPITLQALATESIYAANVGTD
                     GVIGAVDKGLDLAMIGSLLNNLSMSLVAAKPYKTFDDLRGKTIGSQTITTGTGFAMRL
                     VLRAHGLEYPRDYQILNIGGVTDRYVALQSGQIAATPLSVPLDISAKQQGFNVIGYFA
                     DDLPTYFFNPYTIKRSLAEKNRPLIVRYMKAIVQTHRWLFDNHESACAYLSKEMAMTV
                     DNCRIAWEYSVKNRVWDRNADISLEGVRTMIKIVAEINGQKDPLPQPAKYIDQSYIRQ
                     ALSELPNK"
     gene            1576..2523
                     /locus_tag="VMZ02_00230"
     CDS             1576..2523
                     /locus_tag="VMZ02_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70376.1"
                     /translation="MAGVRLEAQEKVRFPIGESSKTLSYGPLWVAAKMGFFEKEGLDV
                     PIVTMRGSPLTIQALTADSIYVANATVDTLINAYEKGGADLTMIGGLINGLGLSMVGG
                     KQYKSYADLRGTTIGTQTLTSGTGFALRLVLKVHGMEYPRDYQLLNIGGASDRYQALT
                     SGQISSAPVGVPLDLVAKQQGFNILGYFADDLPNFQFNTYIVKRSWAEKNRPLAVRFM
                     KAMVSTMRWMNDNRDQACAYLTKEMVLSMEHCRHGSDYNWKSRIWDRNADLNVEGVRT
                     VMKITADQGILKEPLPPASKYIDQSYLKQALAEIEKNKK"
     gene            2548..3552
                     /locus_tag="VMZ02_00235"
     CDS             2548..3552
                     /locus_tag="VMZ02_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70377.1"
                     /translation="MTTRYSFTSFGLTILIGLSLTHLGLAQDKIRMGLSSVSGLHTAV
                     WVAETKGLFRKHGLDVEVIVTGQGGTAGISALIANDVQMVNSAGDVLVAAALRGGDTV
                     MVAGVVNKGLQRLITKPDIKTPADLKGKRIGVTRIGAVSHSVLLMMLQRWKMNVNDVQ
                     VMQLGSSPSMLATLDKGGIEGAVVTIPSMFVAEDRGYRVLLDMADTDIYYLHTMVAST
                     RQYIKNNRDKVLRFLKGYVEGIAFVKHNRQESVDIVKKKLRIGPEQEKTLERSIDLLV
                     AKYYESVPYPSLRGVETVLGFIEKDNPKAKTADPKSFVDDSLLREIEQSGFVKTLYAK
                     "
     gene            3648..4487
                     /locus_tag="VMZ02_00240"
     CDS             3648..4487
                     /locus_tag="VMZ02_00240"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HUR70378.1"
                     /translation="MAATLWKEYKTVRVEKEDGTTWCILNRPEKRNAMNPQLHYDMLE
                     ALTELEVDKETQVLVLTGAGPSWCAGQDLREYFRGTDNNALERRKASWASQEWRWRRL
                     FYFPKPTIAMVNGFCFGGAFTPLIACDFAIAADDATFGLSEINWGIFPGGLVSRVLAD
                     AMSYRDAMWYIMTGDPFDGKRAAEVKLVNFSVPKEKLREETIKLADKLKKKNPAVLRA
                     AKEVYKHCRTMEYSQAEEYMSAKGTALRLTDPEKGRETGMEQFLDKKTYRPGLGEYKR
                     DAK"
     gene            4604..5764
                     /locus_tag="VMZ02_00245"
     CDS             4604..5764
                     /locus_tag="VMZ02_00245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR70379.1"
                     /translation="MDFNLPEELQLLKETVRKFVDRELIPLERECRPEGEDMPEQFIK
                     PLQEKARAIGLWLLDVPQEYGGAGLDLMSRCVIMEEVARTIAIPFRYAPIFGPEVRPV
                     LFHCNDEQKKRFLQPVIEGKIKVCFAQTEPDAGSDPAGMKTRAVKDGDHYVINGTKRF
                     ITAAKNADYAQVIAVTDPVKGARGGVSCFMVSMKAPGVTLERLWPTMMGDTPGQIHFE
                     NVRVPAADMIGGEGQGFAIAQRWIEEGRVRGHGARPVGIASRALDMMIEYSKVRTTFG
                     QPLANRQAIQFMIADSAMELHACRLMVYECAWRHDRGEDIRHLSYMTKIQCAEMASRV
                     VDRSIQVHGGLGLMRELPLEWWYRQVRSLRITEGTPEVLRWRLAEHIIRNHK"
     gene            5853..7061
                     /locus_tag="VMZ02_00250"
     CDS             5853..7061
                     /locus_tag="VMZ02_00250"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUR70380.1"
                     /translation="MIEIDNTASLLSGYRVLDLSSSMGSFCGKVLRDLGMEVIKIEPP
                     GGDAGRSEPPFAQGHAYPENSLRFAYLNAGKKSISLDIGKDSGRKIFLDLVAKSDIVL
                     ETFEPGEMAARGLGYAELLEKQGKLILVSLTGFGQDGPYAHYKAPDIVGNAMGALLYI
                     SGDPQMTPCNPPETQAYYYASLMACYGTMLALWQREMRGIGAWIDGSVQASMALHEHV
                     AFNYAAEKRVMKRAGSQHQHNAPANLFQCKNGWIALFVTQTHWPLLLKVWDNHEPELD
                     DPKWINSNLRRKEADYINAQVTSFTSRYTKEDLAELMQKNGIPGLPVNSPSDFMKDPH
                     IQARNFFSEVTHPVLGTFAAPGSFFTVDGKRAGPAPAPLVGQHNSEILGGELGLSAEK
                     LAALTAEKII"
     gene            7175..>7838
                     /locus_tag="VMZ02_00255"
     CDS             7175..>7838
                     /locus_tag="VMZ02_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUR70381.1"
                     /translation="MTTTNEILKGVRILAFTTGYAGPYAGRLLANYGAEVIKIESRKG
                     GLDTFRHYGQHKDIDEAPRFIECNLGVRSLAINLKNPTGARLIRELAGKSHAILQNFR
                     PRVLDKLGLGDEELRKVNPKLVIVKLPGFGSEGPKSSYGTWGFNLTAFSGITYLWNHP
                     EQDRPIGSQGVYPDHLGFVLGPTMMIAALLHSRQSGKGVTIDLAQIEGTAAVLGTTYL
                     ETS"
BASE COUNT         1833 a         2043 c         2302 g         1660 t
ORIGIN      
        1 gacggtcttc ggcatcaacg gcttcctacc gattcatacc gccggcaatc gcggcgaaca
       61 ggcgctgtct tcgttagccg aagtgatcaa gatgcatcat ggcgtcagcc atctggacgt
      121 cggcagtttt ggcggcggca cgccgctgat cgccttaggc attgaggacg ccgagatcat
      181 ggcgcgcgac agcatcacca agaggcaagt caaagattat ctctgggagc atgcctcgtt
      241 gaaattttcc gagattcccg cgcgccgcaa aggccataag tcggatgacg aattgatgcg
      301 cgagtcgatc aatatgacgc cggacggcgt ggtgcatcta tcgaccaaac cggaagacat
      361 catggtcatc gttgtcggcg gcaagcatcg ccactcggtg tttctgccga tgtggaccgg
      421 gcgcaatacg ctgtgtgcga tcaaaggaat taaatagttg gagtgatgga gtggggagtc
      481 atgggagggc ggccgagaat agacgatgga ggattgagaa ttgccagaac gttggcgatg
      541 gttgcggttg ctttactacg ggtggtgcct tgcacggcgc aagacaaagt ccgtttcccc
      601 attggtgctt cgtcgaaaac cttcagctac ggtccgctct ggctcggcca gaaattgggt
      661 tttttcgagc gcgagggttt ggatgtccag attgtggtta tgcgcggcac gccgatcacg
      721 ctgcaagcgc ttgccacgga gtcgatctat gccgccaatg tcggcaccga tggagtgatc
      781 ggcgccgtcg acaaggggct tgacctagcg atgatcggca gcctgctcaa caatttgagc
      841 atgagcttgg tcgccgccaa accttacaaa actttcgacg atctgcgcgg taaaaccatc
      901 ggttcacaaa ccattacgac gggaaccggc tttgcgatgc ggctggtgct gcgcgcccat
      961 ggtctcgaat atccgcgcga ctatcagatt ctcaacatcg gcggcgttac cgatcgctat
     1021 gtcgcactgc aatccgggca aatcgccgcg acgccgttga gcgtgcccct cgatatttct
     1081 gccaagcagc agggcttcaa cgtgatcggc tactttgccg atgatctgcc gacctatttt
     1141 ttcaatccgt atacgattaa acgctccttg gctgagaaaa accggccgct gatcgtgcgc
     1201 tatatgaaag cgatcgtgca aactcaccgc tggctgttcg acaatcacga gtccgcttgc
     1261 gcttatctgt cgaaggaaat ggcgatgact gtggacaatt gtcgaattgc ctgggaatac
     1321 agcgtcaaaa accgcgtctg ggaccgcaac gccgatatca gtctggaagg cgtgcgcacg
     1381 atgattaaaa tcgtcgccga gatcaatggc cagaaagatc cgctgccgca gccggcgaaa
     1441 tacatcgacc aaagctatat aaggcaggcg ttgagtgaat tgcccaacaa gtgaaacgtg
     1501 gaatcgctta atcaggagcg gggagatcgg atgaagatgc gctgcttgac gggctgcttg
     1561 ataatacttt tctgcatggc cggagttcgc ctcgaagcgc aggagaaggt tcgttttcct
     1621 atcggtgagt catccaagac gctgagctac gggccattgt gggtagcggc gaagatgggg
     1681 ttttttgaaa aggaaggctt agacgtccct atcgttacga tgcgcggctc gccgttgacg
     1741 atccaggcgc tcaccgcgga ttcgatctac gtcgccaacg cgacggtcga cacgctgatc
     1801 aatgcttacg aaaagggtgg cgcggatctg acgatgatcg gcgggctgat aaatgggctc
     1861 ggcttgagca tggtcggcgg caagcagtac aagagctatg ccgatctgcg cggcacgacc
     1921 atcggcacgc agacgctgac ttccggcacc ggcttcgcat tgcgattggt cctcaaagtg
     1981 cacggcatgg aatatccgcg cgattatcaa ttgctcaaca tcggcggcgc gtcggatcgc
     2041 tatcaagcgt taacctcggg gcagatctcc tcggcgccgg tcggtgtgcc cctcgattta
     2101 gtggcgaagc aacagggttt caatatcctc ggctattttg ccgacgatct gcccaatttc
     2161 caattcaaca cgtacatcgt caagcgctcc tgggccgaga agaaccggcc actggcggtg
     2221 cgattcatga aagcgatggt atcgaccatg cgctggatga acgacaatcg cgatcaagct
     2281 tgcgcgtatt tgactaagga gatggtgctc agcatggaac actgccgcca tggatcggat
     2341 tataattgga aaagccgcat ctgggaccgc aatgccgatt tgaacgtcga gggcgtgcgc
     2401 accgtcatga aaataaccgc ggaccagggc attcttaaag agccgctgcc gcccgcgagt
     2461 aaatatatcg accagagtta cttgaagcag gccttggcgg aaattgaaaa aaataaaaag
     2521 tagaaatcta aaaaggaaaa cacggccatg accacgagat attcattcac cagctttggt
     2581 ttgacgatac tcattggctt gtctctgacg catttgggcc ttgcccagga caagattcgc
     2641 atgggattga gttcggtgag cggtttgcac actgctgtgt gggtggctga aacaaagggt
     2701 ctgtttcgca aacacggatt agatgtcgag gtgatcgtca ccggccaggg cggcaccgcc
     2761 ggcattagcg cgctgattgc caacgacgta cagatggtaa actcagccgg cgatgtcctg
     2821 gtcgcggctg cgctgcgcgg cggcgatacc gtaatggttg ccggtgtcgt caacaagggg
     2881 ctgcagcgct tgattaccaa gcccgatata aaaactcccg cggatctcaa gggcaaacga
     2941 atcggcgtca cccgcatcgg cgcggtgtcg cattccgtgt tgttgatgat gctgcagcgc
     3001 tggaagatga acgtcaacga cgtccaggtc atgcagttgg gctcgtcgcc gagcatgctc
     3061 gcgaccctgg acaagggagg catcgaagga gctgtagtaa cgattccttc gatgttcgtc
     3121 gccgaagacc gcggatatcg agtgttgctc gatatggccg ataccgacat ttattatctg
     3181 catacgatgg ttgcgtcgac acgccaatat atcaagaata accgcgacaa ggtgctgcgc
     3241 tttctcaagg gttatgtcga aggaatcgcc ttcgtcaaac ataacaggca agaaagtgtg
     3301 gacatcgtca agaagaagct gcgcatcggt cccgagcagg agaagactct ggaacgctcg
     3361 atagatttgt tagtcgcgaa atactacgag tcggtgcctt atccgtcgtt gcgcggcgtc
     3421 gaaactgtgc tcggcttcat cgagaaggac aatcccaagg cgaagactgc cgacccgaaa
     3481 tcgttcgtcg acgatagttt gctgcgcgaa atcgagcaga gcgggtttgt gaagactctc
     3541 tatgcaaaat agccgacgcc agcgaaaaac tattgcggca atccgcttga cagtttcccg
     3601 acggctcgca taagaccata ggtatattta acaaggaggg ctaaagaatg gctgcaacat
     3661 tatggaaaga atataagacc gttagggtcg aaaaagagga cggaacgaca tggtgtattc
     3721 tcaatcggcc ggagaagaga aatgccatga atccgcagct ccactacgat atgctcgaag
     3781 ccttgactga actcgaagtc gacaaggaaa ctcaagtttt ggtgctcacc ggcgcgggtc
     3841 cgagctggtg cgccggccag gatttgcgcg aatatttccg tggaacagat aacaatgcgt
     3901 tggagcggcg caaagccagc tgggcatcgc aggaatggcg ctggcgccgg ctgttttact
     3961 ttcccaagcc aaccatcgcc atggtcaacg gcttttgttt cggcggcgcg ttcacgccgc
     4021 tgatcgcttg tgattttgcc atcgctgctg acgatgcgac cttcggtttg agcgaaatca
     4081 actggggtat tttccccggt ggtttggtca gccgggtgtt ggctgacgcc atgagctatc
     4141 gcgatgcgat gtggtacatc atgaccggcg atccgttcga cggcaaaaga gccgcagaag
     4201 tgaagctggt caatttttcc gtgccaaaag aaaaactccg cgaggagacc attaaactcg
     4261 cggacaaact caaaaagaaa aacccggccg tgttgcgcgc ggccaaggaa gtctacaaac
     4321 actgccgcac catggaatac agccaggccg aagaatacat gtcggccaag ggcacggcgc
     4381 tgcgtctcac cgatccggaa aaaggccgcg agaccggtat ggagcagttc ctcgataaga
     4441 agacctatcg tcctggtttg ggcgagtaca aacgcgacgc caagtagtag cgcagctcaa
     4501 ggatctgcgc ggttcaaggt tcaaaagttc aagggttcaa ggttggctgc gaattacggc
     4561 ttggctcgcc aagccgtaat tcgcagttat aaatggagcc gatgtggatt ttaatcttcc
     4621 cgaagagctg caactgctaa aagaaacggt gcgtaagttc gtcgaccgtg agctgatccc
     4681 gctcgagcgc gagtgccggc cggaaggcga ggatatgccc gagcagttta tcaaaccgct
     4741 ccaggagaag gccagggcga tcggtctctg gctgcttgac gtgccccagg aatacggcgg
     4801 cgccggactc gatctgatga gccgctgcgt gatcatggaa gaagtcgcgc gcaccatcgc
     4861 aattccgttt cgctatgcgc cgatcttcgg gcctgaagtc cgcccggtgt tgtttcattg
     4921 taacgacgaa cagaaaaaac gttttttgca gccggtcatt gagggcaaaa taaaagtttg
     4981 cttcgctcag accgagcctg acgccggcag cgatcctgcg ggcatgaaaa cgcgcgccgt
     5041 caaggacggg gatcactacg tcatcaatgg caccaaaaga tttatcaccg ccgcaaagaa
     5101 cgccgattac gcccaggtca ttgcggttac cgatcccgtc aagggcgcgc gcggcggagt
     5161 gagttgtttc atggtcagca tgaaggcgcc gggcgtgacg ctggaaagat tatggccgac
     5221 gatgatgggc gatacgcccg gacaaattca tttcgagaat gttcgggtgc cggcggcgga
     5281 tatgatcggc ggcgaggggc aaggttttgc catcgcgcaa aggtggatcg aagaaggacg
     5341 cgtgcgcggc cacggcgcgc ggccggtggg catcgcatca cgcgctctcg acatgatgat
     5401 cgaatattct aaagtgcgca cgacgttcgg tcagccgctg gccaatcgcc aggcgattca
     5461 gttcatgatt gccgactcgg cgatggagct gcacgcctgc cgcctgatgg tttacgagtg
     5521 tgcctggcgc cacgaccgcg gcgaggatat tcgccatctc tcttacatga ccaaaatcca
     5581 atgcgccgag atggctagcc gcgtggtcga tcgctcgatt caagtgcacg gcggtttggg
     5641 actcatgcgc gagctgccgt tggaatggtg gtaccgccag gtgcgcagcc tgcgcattac
     5701 tgagggcacg ccggaagtgc tgcgctggcg cttggcggag catattattc gcaatcataa
     5761 gtagtgaaga tggttgaggc tggaggatag aacattgcca atggctatcc tcgatcttcc
     5821 atcgtccatc ctccattggt cgaatcccgc acatgattga aattgacaac actgcttcac
     5881 ttctctccgg ctaccgggtc ctcgacttga gttcgtccat ggggtcgttt tgcggcaagg
     5941 tgctgcgcga tctcggcatg gaagtcatca agattgagcc gccgggcggc gacgccggtc
     6001 gatcggagcc gccattcgcc caaggccatg cttatcccga gaacagtttg cgctttgctt
     6061 atctcaatgc cggcaagaag agcattagcc tcgacatcgg caaggacagc ggccgcaaga
     6121 tttttctcga tctggtggcg aagtccgaca tcgttctcga aacattcgaa cccggtgaga
     6181 tggcggcgcg cggtctcggc tatgcggagc tgctcgaaaa gcagggcaag ttgattttgg
     6241 tttcgctcac aggtttcggc caggacgggc cgtacgctca ttacaaggca ccggatatcg
     6301 tcggcaatgc catgggggcg ctactttaca tcagcggcga tccgcagatg acgccgtgca
     6361 atccgccgga aacccaggcg tactactatg ccagcttaat ggcgtgttac ggcacgatgc
     6421 tggcgttgtg gcagcgcgag atgcgcggca tcggcgcgtg gatcgatggc tcggttcaag
     6481 cgagcatggc gctgcacgag cacgtggcat tcaattacgc agctgaaaag cgcgtgatga
     6541 agcgcgccgg cagccagcac cagcacaacg cgccggcgaa tctgtttcag tgcaagaacg
     6601 gttggatcgc gctgtttgtc acacaaactc actggccgct gttgctcaaa gtctgggata
     6661 atcacgagcc ggaattggac gatcccaagt ggatcaacag caatctgcgg cgcaaggaag
     6721 ctgattacat caatgcacag gtgacctctt ttacatcccg ttacaccaag gaagatctcg
     6781 ccgagctcat gcagaaaaac ggcatacccg gcctgccggt gaactcgccg tccgatttca
     6841 tgaaggaccc gcacattcaa gcgcgcaatt tcttcagcga ggtcacccat ccggtactcg
     6901 gcactttcgc tgcgccaggg agctttttca cggtggatgg caagcgcgcc ggacctgctc
     6961 ccgcgccgct ggttggacag cacaactcgg agattcttgg cggcgaattg ggtttgagcg
     7021 ccgagaaact ggcagcgctc acggcggaga aaatcatcta acaatggata attgagaatg
     7081 gagaatgaaa gacggattag gttgacctga ataagtttat cgcagctgtc cgaaaattat
     7141 caattctcca ttatccattt tcaattaatc aaaaatgact accactaacg agatcctgaa
     7201 gggcgtgcgc atattggcct tcaccaccgg ctacgccggt ccttatgcag gcagattgct
     7261 ggccaactat ggcgcggagg tgatcaagat agagtcgcgc aaaggcgggc tcgatacttt
     7321 ccgtcattac ggccaacaca aggacatcga cgaagcgccg cgtttcatcg agtgcaattt
     7381 gggcgtgcgt tctttggcga tcaatctgaa gaatcccacc ggcgccaggc taatccgcga
     7441 actcgccgga aaatcgcatg cgatcttaca gaattttcgc cctagagtat tggataaact
     7501 cggtttggga gatgaggaat tgcgtaaagt taatcctaaa cttgtcattg tgaagctgcc
     7561 gggttttggc agcgaggggc cgaagagcag ctacggaacc tggggcttca atctcacagc
     7621 cttctctggc atcacctatc tttggaatca tcctgaacag gatcggccca tcggcagcca
     7681 gggggtttac cccgatcatt tgggctttgt attaggcccc accatgatga ttgccgcgct
     7741 gttgcatagc cggcagagcg gcaaaggcgt gaccatcgat ctggcgcaga tcgaagggac
     7801 cgctgcggtt ttggggacaa cttatctgga aacttcgg
//
LOCUS       DATLCH010000009         4223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4496413, whole genome shotgun sequence.
ACCESSION   DATLCH010000009 DATLCH010000000
VERSION     DATLCH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4496413"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..753)
                     /locus_tag="VMZ02_00260"
     CDS             complement(196..753)
                     /locus_tag="VMZ02_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HUR70382.1"
                     /translation="MIFIIWGIVFVTGIWIISVYNNLTALRLRVQNAWHQINVQLKRR
                     YDLIPNLVETVKGYMQFEQDTLQRVIDARGKAMVSRGVKQSAEAEGALNESLGKVFAL
                     MESYPDLKSNKNVLDLQEELATSENQIAFARQYYNDLVTQYNTKQQVFPGNLIASFFK
                     FQPSEFFLVADAETALPKVNLSLPR"
     gene            complement(756..1778)
                     /locus_tag="VMZ02_00265"
     CDS             complement(756..1778)
                     /locus_tag="VMZ02_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HUR70383.1"
                     /translation="MTEKPLEQMGLRDLRGIMRQKAPAEAWKHFNGAAETKSTFYRNP
                     RAFQQWLFRQKIFHDVSDPDISIELFGKTLPIPAITAPVGSFILFGKDAEREVAEGTD
                     RAGAMMFVSQAAKFNLHNWRGADKSPLVFMAYMNRGKPEVEEYAKTAEDLGFAAVGIT
                     MDTVKPVKIGDEVPLSTKDGKPRKGHKSTPKDIEWMKTIVKLPVVVKGIMGADDARVA
                     VSSGADALVVSNHGGRILDFNRSALEALPEVVDAVGGKVPVLLDSGIRSGGDIVKALA
                     LGAKAVLIGRPVAWGVGAFGVPGVERVFNILAEEMQRVLVMTGVARVRDVTKEILIRD
                     DWRKVR"
     gene            complement(2043..2894)
                     /locus_tag="VMZ02_00270"
     CDS             complement(2043..2894)
                     /locus_tag="VMZ02_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR70384.1"
                     /translation="MIHTTALAATHMECRNLQESLKVLTELLAFEKISEKPGEATLKH
                     PNTQWQLVVHEAGPDAPPKPMHNHWGVRVVSPEEVDRAYDYLIAHKAEYKLGAIGKPT
                     WSHGSYSCYFVEPGTNGWEIECYEAFNRKQTTAERFGAVKTAHWDQLLPEERFPGRGY
                     VPQGFTHGTLVATDLEVSKNFYADVLGLDVHRFSDHVIYIKHPTTKTFVVCALRQNAP
                     VFSPNFRNTLTVASRDEVKAAYREFSDPGKTLPVRELFDLKETDDGTSFCFRDPGSNC
                     WELATTN"
     gene            3051..4082
                     /locus_tag="VMZ02_00275"
     CDS             3051..4082
                     /locus_tag="VMZ02_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR70385.1"
                     /translation="MQKIEMRILALVILSLQLHLPVMVEAQENFYAGKTIRIIVGTSA
                     GGGFDTYTRTIARHFGKHIRGQPSIQVENMAGAGHLIAANHMYKVAKPDGLTIGHFHG
                     GWFLYQLFKRPGIEFDAVKFEFLGSPIKESRACAFTKASGITSVERWLAAKSPVKVGG
                     IGGGAPDDIANMLAATTALPIQLVAGYKGTSEIRLAAESGELAGGCWTWDSIRSTWSK
                     AIQSGEAIVVLQAVSKPHPELPNVPLAQSLAKTEEARQLMQAGIQDPADYYRPYVAPP
                     RTPKARVETLRRAFEATMKDPEFLADAQKAKLDIEPITGSEMEIIVGKLFNLSPATAA
                     KLNNILGAK"
BASE COUNT          957 a         1210 c         1112 g          944 t
ORIGIN      
        1 cgcgaaactt agtgccacca cggtggcgat cgggaatggc gcgccggcga acgtaaagcc
       61 gtcgacatac aaataatcga cggcgccgcc gatactcaga aacacaatga taaagaaccc
      121 gatcagcgca gcgctgcagc gctgattatg ggtctggcgt tgcgcgaaat cgatcaacgg
      181 tttggatagc gccgcttatc gcggcagcga cagattgact ttcggcagcg ccgtctccgc
      241 atcggccacc aaaaaaaact cactcggttg gaacttgaag aaactagcga tcagattgcc
      301 ggggaacacc tgctgtttgg tgttatattg agtcactaaa tcattgtagt actggcgtgc
      361 aaaagcgatc tggttttcag aagtggccaa ttcttcctgc agatcgagga cgtttttgtt
      421 ggatttcaag tcgggatagc tttccatcag cgcaaaaact ttgccgaggg attcgttcaa
      481 cgcgccctcc gcttcggcgc tttgcttgac gcctctcgag accatcgctt tgccgcgcgc
      541 atcgatgact ctttgcaacg tgtcttgttc gaactgcatg taacctttga cggtttccac
      601 gagattagga atcaaatcgt aacgccgctt caattggacg ttgatttgat gccaggcgtt
      661 ttgcacgcgc aagcgcagcg cggtcaaatt attatagacc gaaatgatcc agataccggt
      721 gacaaatacg atgccccaga taatgaagat catgcttatc gcacctttct ccaatcatcg
      781 cgaatgagaa tttcttttgt cacgtcgcgc acccgcgcga ctcccgtcat caccagcacg
      841 cgctgcatct cttcggccaa tatattaaac acccgctcga caccgggaac gccgaaagcg
      901 ccgacacccc aagctaccgg acggccgatc agcacggctt tagcgcccaa agccagagct
      961 ttgacgatat cgccgccgct gcgaatgccg ctatcgagga gcaccggaac tttgccgccg
     1021 acggcgtcga cgacttccgg gagagcttcc aacgccgagc ggttaaaatc cagaatgcgt
     1081 ccgccgtgat tggaaaccac cagcgcgtcc gcgccggaac ttaccgccac ccgcgcatcg
     1141 tccgcgccca ttatgccttt tacgaccact gggagcttga cgatcgtttt catccattcg
     1201 atgtctttgg gcgtcgactt gtgacctttg cgcggcttgc cgtccttggt cgacagcggc
     1261 acctcgtcgc cgatcttaac tggcttgacc gtatccatgg tgatgcccac agcggcgaaa
     1321 cccaagtcct cggcggtttt agcgtattct tcgacctctg gtttgccacg gttcatgtaa
     1381 gccatgaaca ccagcggtga tttatcggcg ccacgccagt tgtgtaagtt aaacttggcc
     1441 gcctggctga caaacatcat ggcgccggca cgatccgtcc cctcggctac ctcgcgttcg
     1501 gcatccttgc caaacaggat gaaactaccc acgggcgccg taatcgcggg gatcggcagg
     1561 gtcttgccga acagctcgat ggaaatatcc ggatcgctga catcgtggaa aatcttctga
     1621 cgaaataacc attgctggaa cgcccgcggg ttgcgataaa acgtcgactt ggtttccgcc
     1681 gctccattga aatgcttcca tgcttccgcc ggcgccttct gacgcataat cccgcgcaga
     1741 tcgcgcaatc ccatttgctc caacggcttt tctgtcatga atttcccgcc ttgctgttta
     1801 gctgaatatt ataaaccgct tggggccatt tctctgcggt aattcggtag agggtggccg
     1861 tcggtcacgt tcgatacttg ccccggtgga ggcagtgtcg caatgggcaa ttggcgcatc
     1921 tgtcatcccg aggggcgcaa cgagtgatct cgtgtgcacg cagatttgag agccctcggt
     1981 gtcgcctcgg aatcgcagtt acaacgcatt acccggcgaa gtggatgcaa gaaaagttga
     2041 aatcaattgg tcgtcgcgag ctcccagcaa ttgctgccgg gatcgcggaa gcaaaaggac
     2101 gtaccatcgt cagtttcttt aagatcgaat aattcgcgca cgggcagagt cttgcctgga
     2161 tcgctaaatt ctcgataagc cgcttttacc tcgtcccgtg acgcaaccgt tagcgtattg
     2221 cgaaagttcg gcgagaacac cggtgcattt tgccggagcg cgcagaccac gaaggtctta
     2281 gttgtcggat gcttgatata aattacgtga tcgctgaatc gatgaacatc caagcccaaa
     2341 acatcggcgt agaaattctt gcttacctcc aagtcggtcg ccaccagggt accatgcgtg
     2401 aagccctgcg gcacgtaacc acggccgggg aatcgctctt ccggcaacaa ctgatcccag
     2461 tgcgccgtct tcacggcgcc gaagcgttcc gccgtcgtct gtttacggtt aaaagcctca
     2521 tagcactcga tctcccagcc attggtgcct ggttcgacga agtagcagga ataagagcca
     2581 tgactccacg tcggcttacc gatggcgcca agtttgtact ccgccttgtg cgcgatcaag
     2641 taatcgtaag cgcggtcgac ctcttcgggg ctgactacac gcacgcccca gtgattatgc
     2701 atcggtttcg gcggcgcgtc cggcccggct tcatgtacta ccaactgcca ctgtgtattg
     2761 ggatgtttga gtgtcgcctc accgggcttc tctgatattt tctcaaatgc cagcagctca
     2821 gtcaggactt ttaaagactc ttgcaaattg cggcactcca tgtgagtcgc cgccaatgcg
     2881 gtggtgtgaa tcatagccga tctcctaagt aagctcgcgt gaattatcca agcggcgtca
     2941 ctggcgaaaa cttacagccc gcctgcaggg ctgtcaaact cgccaattcg tcttgacaca
     3001 aagatacctt ttccttacat tcgccgagcc tttgccgccc gggggtaacc atgcagaaga
     3061 tcgaaatgag aattctcgct cttgtcattt tgagcctgca attacatctc ccagtcatgg
     3121 tcgaggccca ggaaaatttt tacgcgggaa aaaccatccg catcatcgtc ggcacctcgg
     3181 cgggaggtgg tttcgacacc tacacgcgga ccattgcgcg ccatttcggt aaacatatac
     3241 ggggacagcc gtcgatccag gttgaaaaca tggccggcgc tggccatctg atcgccgcca
     3301 atcatatgta taaagtcgcc aagcctgacg gactgaccat cgggcacttt catggcggct
     3361 ggtttcttta ccaattattc aaacggccgg gcatcgaatt tgatgctgtc aagtttgaat
     3421 ttctaggctc gccgatcaaa gaaagccgcg cctgtgcatt taccaaagcc agcggtatca
     3481 ccagcgtcga aaggtggctt gcggcaaaat ccccggtcaa agtcggcggc atcggcggtg
     3541 gcgcaccgga tgatatagca aacatgctag ccgccaccac ggcattaccg atccagctgg
     3601 tggccggtta taaaggcacg tccgagatcc gccttgccgc cgagagtggc gagctcgccg
     3661 gtgggtgctg gacctgggat tcaattcgct cgacgtggag taaagcaatt caatcgggcg
     3721 aagcaatcgt cgttcttcaa gccgtctcaa aacctcaccc cgaactgccc aacgtgccgt
     3781 tggcgcaaag tttggccaag acggaggaag cgcgtcaact gatgcaagcc ggcatccagg
     3841 atccggcaga ttactaccga ccctatgtcg cgccgccgcg cacccccaaa gcgcgcgtcg
     3901 agacactgcg ccgggccttt gaggcgacca tgaaagatcc ggagttctta gccgatgcgc
     3961 agaaggccaa actcgatatc gagccgatca cgggtagtga aatggaaatc atcgtcggca
     4021 aactcttcaa cttgagcccg gcgaccgcag ctaagttgaa caacattctc ggtgccaagt
     4081 agattatcag cgcgggcgcg tcgtctaaat ctcaggaggt tcgcatcgat gaaatccaaa
     4141 attaccgcac tgattatggt gctagtgagt gcgatcggag cggcgtctgc gttcgctcaa
     4201 agtaattttt acgaaggcag aac
//
LOCUS       DATLCH010000010         3938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_27589, whole genome shotgun sequence.
ACCESSION   DATLCH010000010 DATLCH010000000
VERSION     DATLCH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27589"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VMZ02_00280"
     CDS             <1..809
                     /locus_tag="VMZ02_00280"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUR70386.1"
                     /translation="SVTVTFPDSDVTPYDKSTSSSRATFHMGKAAQLACAKIREELFR
                     AGARTLEARGEDLELRDNRLQVKGVPEKNLTYPQLFKAIYGEASGSLFGSHTLRTEGG
                     VDAVTGQGKGSSFWFYSAAGAEVEVDTETGKVRILRMTTAVDVGKAIHPLQCNLQNEG
                     SALAGLGSALFEEMLYDRGQPINSSFLDYLLPSMLDHPGEFQSVLVETPHPDGPHGAK
                     GMGEAALPPMAPAIGNAIANALSGVRVRDLPVKPHKIVAALNGSPGDTLK"
     gene            806..1267
                     /locus_tag="VMZ02_00285"
     CDS             806..1267
                     /locus_tag="VMZ02_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUR70387.1"
                     /translation="MTLAIAATINGQRAEFGVLPHQTLLEFLRDDLKLKGAVEGCSVG
                     VCGSCTVLLDGKPVSSCLTLASNIEGKEVTTVEGLSQNGELDPVQQAFLKHQAFQCGF
                     CTTGMIMAVKGLLNAHAHPSEEQARDYLSGNLCRCGTYKEVLAAVQELSGR"
     gene            1326..2213
                     /locus_tag="VMZ02_00290"
     CDS             1326..2213
                     /locus_tag="VMZ02_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR70388.1"
                     /translation="MKFLLLFSLFVVTLLPLNPMLDGAETAAATPVQLKWLGTAGWEI
                     RVADVVILVDPFLTRREAAAGVEWKTNEEEVNRIITKADFIFAGHSHADHIADIPVIA
                     KRFGAKVIGSRTTTNIALTAGIDKAQLMTIRGGEKLEFNDFSVQVIESQHGVLNRGGR
                     RRQPKPEEVLRPWSGAINGDAFVEGGSYLYYFSFGGLRLLHQSTGNFIEENLTGLQPD
                     VAILAENGNYHWNDALKILRPKTVIIHHYDEWRTPFTDGIPDSNRRRAQRFERLIKSW
                     NKQVKVIIPELLEPFDLPQ"
     gene            2229..3023
                     /locus_tag="VMZ02_00295"
     CDS             2229..3023
                     /locus_tag="VMZ02_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70389.1"
                     /translation="MSADICYWKHDIEPSDAFPCYDGKTRVCSRCCVEHCPKETPEWF
                     GACARAGHPTWPSVDRGRRVPSKLVVLESYWDSRLFQTKSVKGFFESMGPLLDPPLQL
                     AHRFVESQRGLAHYTAKPDGLLWRDPGAGDAPIFYLAFHGAPSQVISVLDSIGEQELC
                     DAFTGFGVKPNLVYFAACSVLRDYKGRQFAKKFLEACGCRAVLGYTTDVNWMLSLVTD
                     ILFLHRFYRDDDPWKNLRKIYESVLNDFKPAGEIGWTLIENKSKSA"
     gene            3144..>3938
                     /gene="ychF"
                     /locus_tag="VMZ02_00300"
     CDS             3144..>3938
                     /gene="ychF"
                     /locus_tag="VMZ02_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HUR70390.1"
                     /translation="MLRAGIVGLPNVGKSTLFNAITRTRKAEAANYPFCTIDPNVGMV
                     TVPDGRLQVLQQMVKTNVVIPAVIEFVDIAGLVKGASQGEGLGNKFLTHIREVDAIVQ
                     VVRCFEDPDVHHVSGNIDPVRDIEVINTELMLADLETVKKRRERIAKEIKRGDKSAAI
                     EDAVLVKIENALDAGKPALTVDLTPEEHALSAQFFLLSDKRTIFACNVKESDLATADQ
                     NPYVIKVREYVKTHLACEAVVISAQIESDLVDLETAEAELFLKELGV"
BASE COUNT          922 a         1127 c         1073 g          816 t
ORIGIN      
        1 agagtgtaac cgtgactttt cccgacagcg acgttacgcc ttacgacaaa tccacgagtt
       61 cgagccgtgc gacgtttcac atgggcaaag cggcgcaatt ggcatgtgcc aagatccgtg
      121 aggaattatt tcgcgcgggc gcgagaacgc tcgaagcgcg cggcgaagat cttgagctgc
      181 gcgacaatcg attgcaagtc aaaggcgtgc ccgagaaaaa tttaacctac ccacaactct
      241 tcaaggcgat ctatggcgaa gccagcggca gcttgttcgg cagccatacg ctgcgcaccg
      301 aaggaggcgt cgacgctgtc accggccaag gcaaaggatc gtcgttctgg ttctattcag
      361 cagccggagc cgaggtcgaa gtcgataccg agaccggcaa agttcgcata ttgcgcatga
      421 ccaccgccgt cgatgtcggc aaggcgatcc atcccttgca atgcaatctg caaaacgaag
      481 gctccgccct ggccggtctg ggctcggcgc tttttgaaga gatgctctac gaccgcggcc
      541 agccgatcaa cagcagtttt ctcgattatc tcttgccatc gatgctcgat catcccggtg
      601 agtttcaatc cgtgctggtc gagacgcccc atcccgacgg tccgcacggc gccaagggta
      661 tgggcgaggc cgctctgccg cccatggcac cggcaatcgg caacgcgatc gcgaacgcat
      721 tgagcggcgt gcgcgtgcgc gatcttcccg tgaagccgca caagattgtc gcggcattaa
      781 atgggtcgcc gggagacaca ctgaaatgac gctcgccatc gccgccacga tcaatggcca
      841 acgcgcagag ttcggcgttt tgccgcatca gactctcttg gaattcctgc gtgacgacct
      901 aaagctcaaa ggcgctgtcg aaggctgcag cgtcggcgtc tgcggctcct gcacggtact
      961 cctcgatggc aagcccgtca gttcttgttt gactcttgcc agcaacatcg aaggcaaaga
     1021 ggttacgacg gtcgagggac tctcgcaaaa cggcgaactc gacccggtcc aacaagcctt
     1081 tttaaagcac caagcctttc aatgcggctt ctgcacgacg ggcatgatca tggcggtcaa
     1141 gggacttctc aatgcgcacg cgcatccgag tgaagaacag gcacgtgatt atctgtccgg
     1201 caatttgtgc cgctgcggca cctacaaaga agtattagcg gcggtacagg agctgagcgg
     1261 cagatagtgc tgccaagaat caacggacat atgaataccg acgacgagcc attggagcgg
     1321 ggcaaatgaa gtttttactg ttgttttcgc tgttcgttgt cacactcttg ccattgaacc
     1381 cgatgctaga cggcgccgaa acggcggcag caacacccgt tcagctgaaa tggctcggca
     1441 ccgcgggctg ggagattcgt gttgctgacg tggtgattct agttgatcca tttctcacta
     1501 gacgcgaggc ggcagccggc gtcgaatgga aaactaacga agaggaggtg aaccgaatta
     1561 tcaccaaggc tgacttcatc ttcgctggtc atagtcatgc cgatcacatc gccgatattc
     1621 ccgtcattgc caaacgcttc ggtgccaaag tgatcggctc gagaacaaca accaacatcg
     1681 ctttgaccgc agggatagac aaagcacaac tgatgaccat ccgtggcggt gagaaactgg
     1741 agttcaatga tttctcggtt caagtaatcg aaagtcagca tggcgtactc aaccgcggtg
     1801 gtcgccgccg gcagcccaaa cccgaagagg ttttgcgtcc ctggagcgga gccatcaatg
     1861 gcgatgcctt tgtcgagggg ggcagctatc tctattattt cagtttcggc ggtttacgct
     1921 tactgcacca gagcaccggc aattttattg aagagaacct cacggggttg cagcccgatg
     1981 tcgcaatcct cgcggagaac ggcaactacc attggaacga tgcgctgaaa atactgcggc
     2041 ccaaaaccgt gatcattcac cattacgatg aatggcgcac ccctttcacc gatggcatac
     2101 ccgattcgaa tagacgaaga gcccaacgtt ttgaacgact gattaaatca tggaacaagc
     2161 aagtcaaagt gatcattccg gagctcctcg aaccgtttga tttgccgcag taatcataaa
     2221 ccatccgtat gtctgccgac atttgttatt ggaaacacga catcgagccg agcgacgctt
     2281 tcccttgcta cgacggcaag accagggtgt gtagccgctg ttgcgtcgag cattgcccga
     2341 aggaaacacc agagtggttc ggcgcctgcg ctcgcgccgg gcatcccact tggccaagcg
     2401 tcgaccgcgg ccgccgagtg ccttcgaaac tggtggtttt agaatcctat tgggacagcc
     2461 gcttgtttca gactaaaagc gtcaagggtt tcttcgagtc catgggaccg ctgctcgatc
     2521 cgccgctcca actcgctcac cgcttcgtcg agtcgcagcg cggattggcc cattacaccg
     2581 ccaagcccga cggcctgctt tggcgcgatc ccggtgccgg cgatgcaccg atcttctatc
     2641 tcgcttttca tggcgcgccg agccaagtga tttcggtttt ggattccatc ggcgagcagg
     2701 aactgtgcga cgctttcacc ggcttcggag tcaaacccaa tctagtgtat ttcgccgcat
     2761 gcagcgtcct gcgcgattac aaaggcagac aattcgccaa aaagtttctc gaagcctgcg
     2821 gctgccgcgc tgtgctcggc tacaccaccg acgtcaattg gatgctcagc ctggtcaccg
     2881 acatactgtt tctgcaccgc ttctaccgcg acgatgatcc atggaagaac ttgcgcaaga
     2941 tctacgagtc agtgctaaat gacttcaaac cggccggaga aatcggctgg acattgatcg
     3001 aaaacaaatc caaatcggct taatccgccc tcatgcgtca gctttaagaa cacccgataa
     3061 cttgctagaa atagttggaa gtcggcctct tactactgcc gcactcgact tcgccatatc
     3121 acgatcataa ggattgcaac accatgctga gagccggaat cgtcggtctg cccaacgtcg
     3181 gcaaatcgac actgtttaac gccatcaccc gcacgcgcaa ggcggaggcg gccaactatc
     3241 ccttttgcac catcgatccc aatgtcggga tggttaccgt gcccgacggc cgccttcaag
     3301 ttttgcagca gatggtcaaa accaacgtgg tcattccggc ggtgatcgag ttcgtggaca
     3361 ttgccgggct ggtcaaaggt gcgagccaag gcgaagggct gggcaataaa tttctcaccc
     3421 atattcgcga ggtcgatgcc atcgtccaag tcgtgcgctg cttcgaagac cccgacgtgc
     3481 accatgtgtc ggggaacatc gatccggtgc gagacatcga agtcatcaat accgaactca
     3541 tgcttgccga tctggaaacc gtgaaaaagc gccgcgagcg gatcgccaaa gagatcaaac
     3601 gtggcgacaa gtccgccgcc atcgaagatg ccgtcttggt gaaaatcgaa aatgcgctcg
     3661 acgccggcaa accggctctg accgtcgacc tcacgccgga agaacacgcg ttgtccgcgc
     3721 agtttttcct gctgtcggac aagcgcacta tttttgcctg caacgtcaaa gaatcggacc
     3781 ttgccaccgc cgaccaaaat ccctacgtga taaaagttcg cgaatacgtc aagacgcatt
     3841 tagcctgtga agcggtggtc atcagcgcgc agatcgagag cgatctggtg gacttggaaa
     3901 cggccgaggc cgagttattt ttgaaagaac tgggcgtg
//
LOCUS       DATLCH010000011         2838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8972061, whole genome shotgun sequence.
ACCESSION   DATLCH010000011 DATLCH010000000
VERSION     DATLCH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8972061"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VMZ02_00305"
     CDS             <1..438
                     /locus_tag="VMZ02_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70391.1"
                     /translation="WKNQTAGEFVRRFVYETLLTSDPGTPIEVSLRRRSSLNDLNAFI
                     GIRPSYWIQLRVSGRGIRVGEKLLDELFGELGFRVEEWVGVQGSETRLGIFATIDAPK
                     LTMVFCLEWLRHKLKCDLLLPVGGNLPVPESHLHAPADDSIHV"
     gene            517..1059
                     /locus_tag="VMZ02_00310"
     CDS             517..1059
                     /locus_tag="VMZ02_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUR70392.1"
                     /translation="MQRLNKILVPTDLSERSRRALAYGCQLAVDNRATLVILHVTNEL
                     NAWEISSEFEMFAGNNARLWPLDRIVSEAFLDLNHFLEPHLELLKQIGTTTKRVVLGN
                     VPERIATMAEDEKADLIILSPQRSRGLRHWLGGGITDRVTRLSPCPVLSITQPLPSQP
                     WRGKLAPLFFGWPKQRAAEI"
     gene            1167..1511
                     /locus_tag="VMZ02_00315"
     CDS             1167..1511
                     /locus_tag="VMZ02_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70393.1"
                     /translation="MTSRQLPKQQKAIAAERNRSPGRRTSPVGRIAHELINQLSVLNL
                     VAYNVIAGDDCGQDARLTRNKEIFQQSVHQATALAEQLARYVDGSLEGSEPIPESGKV
                     VRLLRSMARCDR"
     gene            1616..>2838
                     /locus_tag="VMZ02_00320"
     CDS             1616..>2838
                     /locus_tag="VMZ02_00320"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HUR70394.1"
                     /translation="MAQHFAVIDVGSNAMRCQVAVVDKPKHYRVIEQDRQPVRLGREV
                     FQSGKLNPETVEAALKVLGDFKKLADRFHVKAIRAVGTSALREASDSPAFIRRAAKIG
                     IPLAVLSEEDEARLISLGIMSGLRFHLPLGLFLDIGGGSVELAVANTTNTYCLFSLPL
                     GAVRLTEKHIAHDPPRKREIKELRESARGQLKAVARRIQQEKFTMAFGSGGTITALAE
                     TDQRITGDPNRAGSLTVLRRQRLKTLLELLQNEPAGERAAMISGDPKRADIIVAGGFV
                     LYEIMSEIGLEYIFVSRRGLRDGLMVDLLQQRHAENGPWHPDADRAESLEQVCQKYLY
                     DAAHAHHVSQLALNLFYQLHDLHGLPDRYASILHAAAMLHDIGVFLAEPKHHKHSYYL
                     IKSSGMNSFNRLDLDL"
BASE COUNT          660 a          805 c          768 g          605 t
ORIGIN      
        1 tggaaaaacc aaacggccgg agaattcgtc cgccggttcg tttacgagac cctgcttaca
       61 agcgatcccg ggacgccgat cgaagtttcg ttgcggcgcc gaagctctct caacgacctc
      121 aatgctttca tcggcatcag accgagctat tggattcaac tccgcgtcag cggccgcggc
      181 attcgcgtcg gtgaaaaact actcgacgaa ctgtttggcg aactcggttt tcgagtcgaa
      241 gagtgggtcg gcgtccaagg atcggagact cgattaggga ttttcgctac cattgatgcg
      301 ccgaagctga cgatggtctt ctgcctcgag tggttaaggc acaagctaaa atgcgacctc
      361 ttgctgccgg tcggcggtaa cttgccggtg ccggagtccc atcttcacgc gccggcagac
      421 gattcgattc acgtttaacc ggttgttctt ggcgctgtca tccaacgcta atacaccgac
      481 cttaagctat ctccatcaag accaaaggag aaacctatgc aacgcttaaa caaaattttg
      541 gtacccaccg atctgtcgga gcgctcccgg cgcgccctcg cttacggctg tcaattagcc
      601 gtcgataata gggcgacgct ggtcatatta cacgtcacca acgaactcaa cgcgtgggag
      661 atatcgagcg agtttgaaat gtttgccggc aacaacgccc ggctatggcc gctcgatcgc
      721 attgtcagcg aagcattcct cgacctcaac cactttctcg aaccgcatct ggaactgtta
      781 aaacagatcg gcactacaac caagcgagtc gtcctgggga acgtgccgga acgcatcgca
      841 acgatggccg aagatgaaaa agccgatttg atcatactgt cgccgcaacg cagccgcgga
      901 ctgcgccact ggcttggcgg cggcattacg gaccgagtga cgcgcctaag cccctgtccg
      961 gtgttgtcga tcacgcaacc gctgccgtcg cagccgtggc gcggcaagct ggcgccgctg
     1021 ttttttggct ggccaaagca gcgcgccgcg gagatttaac ctcagaagcg ccgcaggcca
     1081 tagttcaatg acgaccgcac aatgtgcgcc ggaagaaata ttgcgtgatg ctttagaaac
     1141 agttccagag ataaaaagga ttcaaaatga ccagtagaca gttgcccaaa caacagaaag
     1201 cgatagctgc tgaacgcaat agatcccccg gccggcgcac atcgcccgtc ggcaggattg
     1261 cccatgaact gattaatcag ttaagtgtgc tcaatctggt cgcttacaat gtcatcgccg
     1321 gagatgactg tggtcaagac gccaggctca cgcgcaataa agaaatcttc caacaatccg
     1381 ttcaccaagc cacggccctc gccgagcagt tggcgcgtta cgttgatggc tccctcgaag
     1441 gctccgaacc tatccccgag agtggcaagg tggttcgatt gctgcgaagc atggcccgtt
     1501 gcgatcgcta agccaccgcg gtcgctatgt ggaactgggt cattaaccga gaatttaaca
     1561 caatcgtaac ctgataattt ttgaacggtt tcggtttgtg atatgaagcc ggtcgatggc
     1621 acagcacttc gcagtgatcg acgtcggcag caacgctatg cgctgccaag tggcggtcgt
     1681 cgataagccc aaacactacc gcgtcattga acaagaccgc caaccggtgc gcttgggacg
     1741 tgaagttttc cagagcggca aattgaaccc agagacggtt gaggcagcac tcaaagtcct
     1801 cggggatttt aaaaaactcg ccgaccgttt tcatgttaaa gccattcgcg ccgtcggcac
     1861 cagcgccctg cgtgaagctt ccgatagccc ggcattcatt agacgcgcgg ccaagatcgg
     1921 tatcccactc gcagttttat ccgaagaaga cgaagcgcgg ctgatcagtc ttggcattat
     1981 gagcggcttg cgtttccatc tgccgctcgg cttattcctc gatattggcg gcggtagcgt
     2041 cgaactcgcc gtcgcgaata ccaccaacac ttactgctta ttcagtttgc cgttgggcgc
     2101 ggttcgttta actgaaaagc acattgccca cgatcccccg cgtaagaggg aaatcaaaga
     2161 actgcgcgag tctgctcggg ggcaactgaa ggcggtggcg cggcgcatcc agcaggaaaa
     2221 gtttaccatg gcattcggca gcggcggcac aatcaccgct ctggcggaga ccgaccaacg
     2281 tattaccggc gacccgaatc gcgcaggatc gttgactgtg ttgcggcgcc agcggctaaa
     2341 aaccttgttg gaacttttgc agaacgagcc ggctggcgag cgcgccgcca tgatcagcgg
     2401 cgaccccaaa cgagcggata ttatcgtcgc gggtggattc gttctttacg agatcatgtc
     2461 ggaaatcggt ttggaatata ttttcgtctc ccgcaggggt ttgcgggacg gactgatggt
     2521 cgacctgctg caacagcgcc acgcggagaa cggaccatgg catcccgacg ccgaccgcgc
     2581 ggaatcgctc gagcaggtct gtcaaaagta tctttacgat gccgctcacg cacaccatgt
     2641 cagtcaattg gcgctcaatc ttttttacca attgcatgac ctgcacggct tacccgatag
     2701 gtacgcgagc attctccatg ctgcggcgat gctgcacgac attggcgttt tcctcgctga
     2761 gccgaagcat cataagcact cctactatct gattaaaagt tcgggaatga attctttcaa
     2821 tcgcctggat ctcgatct
//
LOCUS       DATLCH010000012         8520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1523603, whole genome shotgun sequence.
ACCESSION   DATLCH010000012 DATLCH010000000
VERSION     DATLCH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523603"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..665)
                     /gene="nrdR"
                     /locus_tag="VMZ02_00325"
     CDS             complement(162..665)
                     /gene="nrdR"
                     /locus_tag="VMZ02_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HUR70395.1"
                     /translation="MKCPFCHEIENRVIDSRLSKDSNMIRRRRECERCTRRFTTYERV
                     EEMMPLVVKKDGRREGYDRVKIINGLKRACEKRPVSINTIEAIADHIERTLQERGEKE
                     IVSSVIGEALMRELHDTDPVAYVRFASVYRSFKDIGEFMVELEELIKERKTVPHFKPG
                     DSRPRRE"
     gene            complement(762..2009)
                     /gene="glyA"
                     /locus_tag="VMZ02_00330"
     CDS             complement(762..2009)
                     /gene="glyA"
                     /locus_tag="VMZ02_00330"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HUR70396.1"
                     /translation="MSFLEQVDAEVFAAIQRETRRLEGNLELIASENVVSEAVLEAQG
                     SVMTNKYAEGYPGKRYYGGCEFVDEVESLAIARAKELFGADHVNVQPHSGSQANMTVY
                     LAVLKPGDTYLGMNLAHGGHLSMGSPVNFSGFIYHVIPYGVSEKTETIDYDEVERLAK
                     EHRPKLIIAGASAYPRIIDYAQFRKIADGIGALLMVDMAHIAGLVAAGLHPSPVPYAD
                     FVTTTTHKTLRGPRGGMVLCKAEHAKIIDSRVFPGLQGGPLMHVIAAKAVALKEALSP
                     DFKLYQQQIVKNAQALAQSLMGHGYRLTSDGTDNHLMLVDLRKSELTGKVAQETLDKA
                     RITVNKNGIPFDTRSPFVTSGIRIGTPAVTSRGMKETEMAQIGGLIGRALGAVGDESE
                     LRAVADDVGMLCKKFPVYPHRLP"
     gene            complement(2021..2500)
                     /gene="rpiB"
                     /locus_tag="VMZ02_00335"
     CDS             complement(2021..2500)
                     /gene="rpiB"
                     /locus_tag="VMZ02_00335"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HUR70397.1"
                     /translation="MIAIGCDHGGVELKDILVGYLRGKGADVCDVGTQGRESVDYPDY
                     GREVSTRVSSGAAERGVLICTSGIGMSIVANKFPGVRAALVADLDGARSSREHNDANI
                     LVLSGAKTEPRLAQEIVETWLATEFAGGRHQRRVAKITSIEQEIGGASTGKRKPSDI"
     gene            complement(2672..2908)
                     /gene="acpP"
                     /locus_tag="VMZ02_00340"
     CDS             complement(2672..2908)
                     /gene="acpP"
                     /locus_tag="VMZ02_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUR70398.1"
                     /translation="MAGSIKDRVKEIVCEQLGVSAEEVTPEASFIEDLGADSLDIVEL
                     VMALEEEYEMEISDEDAEKIKTVQDIITYIEAHR"
     gene            complement(2934..3680)
                     /gene="fabG"
                     /locus_tag="VMZ02_00345"
     CDS             complement(2934..3680)
                     /gene="fabG"
                     /locus_tag="VMZ02_00345"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HUR70399.1"
                     /translation="MASLHGQIALITGASRGIGRAIAVALAQAGAHVIINYKGNQAAA
                     EESLGLVRASNGSGELCKFDIADESQVEAAFKQIVDLHKTVDLLVNNAGVTSDGLLMR
                     MKSADWDQVIGTNLKGTMLCTKVVARQMIKQRAGRIINLSSVVGQMGNVGQSVYAASK
                     AGVIGFTKAMARELASRAITVNAVAPGFIDTDMTAHLPEKLREEFLRSIPLGRFGTCQ
                     EVADMVVFLAGPGGSYVTGQVFNINGGLYM"
     gene            complement(3681..4616)
                     /gene="fabD"
                     /locus_tag="VMZ02_00350"
     CDS             complement(3681..4616)
                     /gene="fabD"
                     /locus_tag="VMZ02_00350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HUR70400.1"
                     /translation="MASSDFVFVFPGQGSQFVGMGKDLCDQFGVAKQVFAEAEDALGF
                     SLTKLCFSGPETDLQLTEHTQPAILTMSVAALRVLEAETDLRPAYVAGHSLGEYSALV
                     AVGALAFRDAVKLVRERGRFMQRAVPAGQGAMAVILGLEMNAVEALCAEASQHEIVAP
                     ANYNGGGQVVIAGSKGAVIRAMTLAKERGAKRALDLPVSAPFHCELMRPAADGLKQVL
                     QNISVEPFTVPIVTNVEAEINRDCHRVKHLLVEQAVRPVRWEESVKKLCALGCQRALE
                     IGPGKVLKGLIKRIAPKLEADNFEKPADLARLKAA"
     gene            complement(4606..5724)
                     /gene="plsX"
                     /locus_tag="VMZ02_00355"
     CDS             complement(4606..5724)
                     /gene="plsX"
                     /locus_tag="VMZ02_00355"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HUR70401.1"
                     /translation="MPKIAVDAMGGDRAPAVVIEGALLARQELGVDIVLVGPPEIIGA
                     ELAKHPSAPAIAIVPASQVVPMHESPSLALRRKNSSMKVAFDMMRGGDADAVVSAGNS
                     GAMMALGMFVMGKLSHVMRPAILVVVPSTSKGTVIVDAGANVDCKPRHLLQFGLMGSI
                     YAESVLGMAKPRVGVLSNGEESTKGTELTRAASALLGATTINYIGYVEGRDIFNGKVD
                     VIVCDGFTGNVALKTMEGVASFAGEVLKSAFEKNVASMVGYLMSRNSLKSAYRKLDYA
                     EYGGAPLVGLDGVAIVAHGGSSALAIKSAIRLARDAVDHEINRRIVTALNNTETGEDI
                     KKEGLPHKIWSRLKAKIDSVGDKPETEDSEDERSRRGE"
     gene            complement(5741..5923)
                     /gene="rpmF"
                     /locus_tag="VMZ02_00360"
     CDS             complement(5741..5923)
                     /gene="rpmF"
                     /locus_tag="VMZ02_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HUR70402.1"
                     /translation="MPVPKRRTSKRVKNQRRAHDHLTAPQWSACANCGETVLPHRACA
                     HCGFYRGRAVIKTENS"
     gene            complement(5984..6520)
                     /locus_tag="VMZ02_00365"
     CDS             complement(5984..6520)
                     /locus_tag="VMZ02_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HUR70403.1"
                     /translation="MKISIDEIPQTPKEIVFSESVEELNKIYSQSSNREFGFPAFLAV
                     ELRYYRAAADLFFDGRISGELRGVCGRCAEEYGFSLDQLFEFVLIPDPAKADRKAEEL
                     HRDDLGLSYYSGDEIDLAPLIAEQVILALPTQPLCAKSCRGLCASCGANLNRESCSCV
                     TPTGDPRMEVFRSLKVGR"
     gene            6613..7722
                     /locus_tag="VMZ02_00370"
     CDS             6613..7722
                     /locus_tag="VMZ02_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HUR70404.1"
                     /translation="MIIQHFHGTRETRTRIKRELGLTCQTGRFLLTLFFQLMNFPVHL
                     HSLWHGKRFNSFNRVLRDSFAARVYKIGLRVDFTCPNRDGTVAVGGCIYCNNASHTPA
                     TYRPRLTVTEQLKQGAAAIRRRHSAERFIAYFQSYTNTYDSIDRLEQFYREALAFPGV
                     IGLAVATRPDCLANETLDLLAELAQHTYLWLELGLESMRDRTLRWINRGHSLAQYIDA
                     VERAHGYGLRICTHLILGFPGESREDMLETADLFNRLSMDGVKLHNLHVLKNTVLEKY
                     YDAGAVPLLSQDDYAALVVDFLERLNPAIVMHRLSAVAHRTLTVAPAWSVDKRGMHNR
                     IHRELIQRDAWQGRLYTKSEFRTSLASMSNQGAAI"
     gene            7719..>8520
                     /locus_tag="VMZ02_00375"
     CDS             7719..>8520
                     /locus_tag="VMZ02_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUR70405.1"
                     /translation="MNGHIGLIHSLLPATVDLQVTIPESHPSARNLGAERVGSGTIID
                     PSGYILTVHYVTIGAASITVTLSDGEEHPGQVVAQDQESGLSLVKISGRDLPFVKLAA
                     PNSLTLGQAVVMIASSGKNGRRVSGGYVSSLAGYDGEWEYMIDKSIRVTAFNPGFGGG
                     TLANFHGELAGVVSLNLNEVGKFTMAIPVEYYQTYEQELKQHGQVRSRPRRPWLGIYT
                     QGVAGLVVIGNVTGNGPAAKCGLKAGDVILSVENNKIRSRQELYHEVWK"
BASE COUNT         1838 a         2518 c         2261 g         1903 t
ORIGIN      
        1 ggcctgatgg tgataaccgg tgccgacgac tttattgccg cgcacgagca ccgcgcccac
       61 cggcggattg gggctagttc ttcccgcagc tttgcgcgcc aaacgacagg ccatgcgcag
      121 gtagcgaatc tcctgccgag atccccggac accggccaac gttattccct tctcgggcgg
      181 gagtcgccgg gtttgaaatg cggcacggtc ttgcgttcct tgatcagctc ctcgagctcg
      241 accatgaatt cgccaatgtc tttgaacgag cggtacacgg aggcaaaccg cacataggcc
      301 actggatcgg tgtcatgaag ctcgcgcatc agtgcttcgc cgattaccga actaacgatt
      361 tctttctcgc cgcgctcttg gagcgtccgc tcaatatggt cggcaatcgc ttcgatggtg
      421 ttgatactga ctgggcgctt ttcacaagcg cgcttgagtc cgttgatgat cttgacccga
      481 tcataacctt cgcgccgccc atcctttttg accacgagag gcatcatctc ctcgacgcgc
      541 tcgtacgttg taaaacgccg ggtgcagcgc tcacactcgc gccggcggcg aatcatgttg
      601 ctgtctttac ttagccgtga atcgataacg cgattctcta tttcatggca gaaggggcat
      661 ttcatggtga ataaatcgag gacgggaaat ggaagacaga ggatcggaaa tactaatcgc
      721 cattctctaa tctcggtcct ccatcaatga cccgacgaat actacggcaa acggtgcgga
      781 taaacgggaa actttttgca cagcatgccg acatcatcag caaccgcgcg caattcgctt
      841 tcatcaccca ccgcgcccaa agccctgccg atcaaaccac cgatctgcgc catttccgtt
      901 tccttcatgc cacgactggt cacggccggc gtgccgatgc gaatcccgct ggtgacaaac
      961 ggtgaacggg tgtcgaacgg aatgccgttc ttgttgacgg tgatgcgcgc cttgtccaaa
     1021 gtctcttggg ccactttgcc ggtcagctcg gactttcgca ggtctaccag catcaagtga
     1081 ttatcggtac catcggaggt caagcgatac ccgtgaccca tgagcgactg cgccagcgct
     1141 tgagcattct tgacgatttg ctgttgatag agtttgaagt ccggactcaa agcctctttc
     1201 agtgccaccg ctttggcggc aattacgtgc ataagcggtc cgccctgcag gccgggaaaa
     1261 acccggctat cgattatttt ggcgtgctct gctttacaca acaccatgcc gccacgcggc
     1321 ccgcgcaagg ttttgtgcgt cgtcgttgtg acaaaatcgg cgtaaggaac cgggctcgga
     1381 tgcaaaccgg cagcgaccaa tccggcgatg tgagccatgt caaccattaa gagcgcgccg
     1441 ataccgtcgg cgattttgcg gaactgcgcg tagtcgatga tgcgcggata ggcactggcg
     1501 ccggcaatga tcaacttggg ccggtgctcc ttggccaagc gttcgacctc atcatagtcg
     1561 atggtctcgg tcttctcgga aacgccgtac gggatcacgt ggtaaatgaa accggaaaaa
     1621 tttactggac ttcccatcga caaatgaccc ccatgggcca agttcatacc gagataagta
     1681 tcaccgggtt ttaacaccgc caggtaaaca gtcatgttgg cctgggaacc ggagtgcggt
     1741 tgcacgttta cgtgatcggc accgaatagt tctttggcgc gcgcgatcgc cagcgattcg
     1801 acttcgtcga cgaattcgca gccgccgtag tatctcttcc ccggataacc ctcggcgtat
     1861 ttgttggtca tcaccgagcc ctgggcttcc aacaccgctt cgctgacgac gttctccgag
     1921 gcgatgagtt cgagattacc ctccaagcgg cgagtttcgc gttgaatcgc ggcaaatacc
     1981 tccgcgtcta cctgctcgag aaatgacatg agttttttcc tcagatatcc gatggtttcc
     2041 gcttgcctgt cgaggctcca ccgatttctt gctcgatact agtaattttg gcgacacggc
     2101 gctggtggcg gccgccagcg aactcagtgg ccaaccaggt ctcgacaatt tcttgagcca
     2161 gccgcggttc ggtcttggcg ccgctcagca ccaaaatatt cgcatcgtta tgttcgcggc
     2221 tagaacgggc gccatccaga tcggctacca aggctgcgcg cacgccgggg aatttattag
     2281 cgacgatgga cataccgatt ccgctggtgc aaatcagcac accgcgctcg gctgcgccgg
     2341 aagaaacccg cgttgatact tcgcggccat agtctggata atccacggat tccctgcctt
     2401 gagtaccgac gtcgcagacg tccgcgcctt tgccgcgcaa gtagccaact aagatatctt
     2461 tcaattccac gccgccgtgg tcgcagccta tggcgatcat gagttcaggc gattcgtacg
     2521 atccgtcgct gccgaaagta atttaaaata aaaaattccg aagcatggtt gccagttacc
     2581 atcgcttcag cgacatcgaa gacgaacttg caatgaggag taccgtagcg ctgacgcccg
     2641 agcgggcgcc attgcggaac ggacaaggcg gttatcggtg cgcctcgatg taggtgatga
     2701 tatcctgcac ggttttgatc ttctctgcgt cttcgtcgga aatttccatc tcatattctt
     2761 cttcgagcgc cattacgagc tcgacgatat ccaacgagtc ggcgcccaaa tcttcgatga
     2821 acgaagcttc cggcgttact tcttcggcac tcacgccgag ttgctcgcag acgatttcct
     2881 tcactctgtc cttaatcgat cccgccatct tcacttacct cctctttcaa tcattacatg
     2941 tataatccac cgttgatgtt aaacacctgt ccggtcacat agctgcctcc cggcccggct
     3001 agaaacacaa ccatatcggc gacttcctgg caagtgccaa aacgccccaa cggaatggag
     3061 cgaagaaatt cctcacgcaa cttctcgggt aagtgcgccg tcatatcggt gtctatgaag
     3121 ccgggcgcca cggcattaac ggtgatagca cgtgaggcca attcgcgcgc cattgctttg
     3181 gtaaatccga tgacgccggc tttggaggcg gcgtaaacgg actgtccgac gttgcccatt
     3241 tgaccgacga ccgaagatag attgatgatg cggccggcac gctgcttgat catctggcgc
     3301 gcgacgactt tagtacacag catcgtcccc ttaagattgg tgccaatgac ttggtcccag
     3361 tcagctgatt tcatacgcat cagcaaacca tccgaggtca ctccggcatt gttgacgaga
     3421 agatcgactg ttttgtgaag atcgacgatc tgcttgaagg ccgcttctac ttgggactca
     3481 tcggcgatat cgaatttgca gagttcgcca ctgccgttag atgcacgcac taggccgagc
     3541 gactcttccg ccgccgcctg gttaccctta tagttgataa ttacatgggc cccagcttgc
     3601 gccagggcta cagcaatggc gcggccgatt ccccggctgg cgccggtgat cagagcgatt
     3661 tggccgtgaa gagatgccat ttacgcggct ttcaacctgg cgaggtccgc cggcttttca
     3721 aagttgtcgg cctccaactt cggggcgatt cgcttgatca aacctttgag caccttgccg
     3781 ggaccgatct ccaaagctcg ttggcagccc aatgcgcaaa gcttttttac cgactcttcc
     3841 caacggaccg gccggaccgc ttgctcgaca agaaggtgct tcacccgatg gcagtcgcga
     3901 ttaatttcag cttcgacatt ggtcacgatc gggacggtga atggctcgac ggagatattt
     3961 tgcaggacct gctttaggcc gtcagccgcc ggccgcatga gttcgcaatg gaaaggcgcg
     4021 ctcaccggta agtcgagggc gcgcttggcg ccgcgctctt tggctagggt catggcacgt
     4081 atcacagcgc ctttagaacc ggctatgaca acttgtccgc cgccattgta gttagctgga
     4141 gcgacgattt cgtgctggct cgcttcggca catagggctt cgaccgcatt catctccaat
     4201 ccgaggatga ccgccatcgc gccttggcca gcgggtactg ctcgttgcat aaaacggccg
     4261 cgttcacgga caagtttcac agcgtcgcga aacgctaaag cccccactgc aaccagagcg
     4321 ctgtactcgc ctaggctgtg gccggcgacg tacgccgggc gcaagtcggt ttccgcttcg
     4381 agcacgcgca gcgcggcgac cgacatcgtc agtatggccg gttgggtgtg ttcggtcagc
     4441 tgcagatcgg tttccggacc ggagaaacag agcttcgtga gggaaaatcc cagcgcgtct
     4501 tcggcctcgg caaatacttg tttggcgacg ccaaattgat cgcacaaatc tttgcccatg
     4561 ccgacaaatt gtgaaccctg gccggggaat acgaatacga aatcgctact cgccacgacg
     4621 gctccgctcg tcttcgctat cctcagtctc aggcttgtcg ccgaccgaat ctatttttgc
     4681 cttgagacga ctccaaatct tgtgcggtag gccttctttc ttaatgtctt cgcctgtctc
     4741 ggtgttgttc aaagccgtca cgatgcgccg attgatctcg tggtcgacgg catcgcgcgc
     4801 taaccgaatc gcactcttga tcgccaatgc gctggaaccg ccatgcgcaa cgatcgctac
     4861 cccgtccaaa ccgaccagcg gggcgccacc gtactctgcg taatccaatt ttcgatatgc
     4921 cgacttcaag gaatttcggc tcattaagta accgaccatg ctcgcaacat ttttttcgaa
     4981 tgcgctcttt aggacttcac cggcaaagct cgcgacgcct tccatggtct tgagcgcgac
     5041 gttacccgtg aagccgtcac acacgatgac atccactttg ccgttgaaaa tatcgcgtcc
     5101 ctcgacgtag ccaatgtaat taattgtcgt cgctcctagc agagcgctgg cggcacgcgt
     5161 cagctccgta cctttggtcg actcctcacc gttgctcaac acgccgacac gcggcttggc
     5221 cattccgagg acgctttcgg catagatcga gcccatcaga ccgaattgca acaaatgtcg
     5281 aggtttgcaa tcgacgttgg caccggcatc gacgatcacc gtgcccttgc tcgtgctcgg
     5341 aacaacgacg agaatcgccg gtcgcatgac gtgcgaaagc ttacccatga cgaacatgcc
     5401 gagcgccatc atcgcgccgg aatttcccgc gctgactacg gcatcggcgt cgccgcccct
     5461 catcatatcg aaggcgactt tcatcgacga atttttcctg cgcaaagcca ggctgggaga
     5521 ttcatgcatc ggcaccactt gggatgccgg aacgatggcg attgcgggcg cactcggatg
     5581 tttggcgagt tcagccccga tgatttctgg gggaccaacc agaacgatgt caacgccgag
     5641 ctcttggcgg gccagcaacg caccttcgat cacaactgcg ggggctcggt ctccacccat
     5701 cgcgtcgacg gcgattttcg gcatagctgg cggagatgga ttagctattt tcggttttga
     5761 tcacagcgcg cccgcggtag aaaccgcaat gagcgcaggc acgatggggc aatacggtct
     5821 cgccacagtt agcgcaggcg ctccattgcg gggctgtcag atggtcatgc gcccggcgtt
     5881 gattctttac ccgctttgaa gtgcgtctct taggtaccgg catctaattg tccaatcttt
     5941 ccccgccccg ggcggcaaca agttcatcca atcgataaag acttcagcgt cccactttca
     6001 agcttcggaa aacctccata cgaggatctc ccgttggcgt tacacaagaa cagctttcac
     6061 gattcagatt agcgccacag ctggcgcata agccgcggca gcttttcgca cacagcggtt
     6121 gcgtcggcag cgccaagatc acctgctcgg caattagcgg agccagatcg atctcgtcgc
     6181 cggagtaata gctcagtccc aaatcgtcgc gatgcagctc ttcggctttg cggtcggctt
     6241 tcgccggatc cgggatcaac acgaactcaa agagctgatc taaactaaac ccatattctt
     6301 cggcacaacg tccgcacacg ccgcgcaatt caccgctgat ccggccatcg aaaaacagat
     6361 ctgccgcggc gcggtagtag cgcagctcga ccgccaaaaa cgctggaaag ccaaactcac
     6421 gattgctact ttggctataa atcttattga gttcctcaac actttcggag aatacgatct
     6481 ctttgggggt ttgaggaatc tcgtcgatgg agattttcat cggcgataat gcgggccgct
     6541 aaaagtaaat attaaatatc ggcaatcacc taaaatgtca agccgacgcg tcggcgtcag
     6601 acggaaaact gcatgattat tcaacacttc catgggacgc gcgagacccg aacgagaatc
     6661 aaacgcgagc tcggtttgac ttgtcaaact ggtcgctttt tgttaaccct ttttttccag
     6721 ctcatgaatt ttcccgtcca cctgcactcg ctctggcatg gcaagcgctt caactctttt
     6781 aatcgtgtgc tgcgcgacag cttcgccgcc cgagtataca aaatcggctt gcgcgtcgac
     6841 tttacctgcc ccaatcggga tggcacagtg gcggttggcg gctgcattta ctgcaataac
     6901 gcgagccata caccggcaac ctaccggccg cgcttaactg tgaccgagca gctcaaacag
     6961 ggcgctgcgg cgatccggcg gcgccacagt gccgagcgct tcattgccta ctttcagagt
     7021 tataccaaca cctacgattc gatcgatcgc ctggaacaat tctatcgcga agccttggcg
     7081 tttccgggtg tcatcggact cgccgtggcg acccggccgg actgcttggc gaacgagact
     7141 ctcgatctcc tggcagaact tgcgcagcac acatatctct ggctcgagct cgggctcgaa
     7201 tccatgcgcg atcgaacact gcgctggatc aatcgcggcc acagcctggc gcaatatatc
     7261 gacgccgtcg agcgcgccca cggctatgga ctccggatct gcactcacct gatcttgggg
     7321 tttcccggtg agagccgcga agacatgctc gaaaccgcgg atttatttaa ccggctcagc
     7381 atggatggcg tgaaactgca caatctccat gttctaaaaa atactgttct agaaaaatac
     7441 tacgatgccg gcgccgtgcc gctgttgagc caggacgact acgccgcgct agtggtcgac
     7501 tttctcgaac gtttaaatcc tgcgatcgtc atgcaccggc tctctgccgt ggcccaccgt
     7561 accctgacgg ttgccccggc ttggtcggtc gacaagcgcg gcatgcacaa tagaattcac
     7621 cgcgaactga tccaacgcga tgcttggcag ggccggctct atacaaaatc tgagtttcgg
     7681 actagcctag cgagcatgtc caaccaagga gcagcaattt gaacggtcat attggattga
     7741 ttcattcgct cttgccggca acggtggatc tgcaagtgac cataccagag agtcacccct
     7801 cggcgcgtaa tctcggtgcc gaacgagtcg gatccggcac catcatcgat ccgagcggct
     7861 acatcctcac ggttcactat gtcaccatcg gcgccgcatc gatcaccgta accttatcgg
     7921 atggtgaaga acatcccggg caagtcgtgg cacaagacca agaatctggc ctgtcgctag
     7981 tcaaaatttc cggacgcgat cttcccttcg tgaaattggc ggcgccaaac tcgctgaccc
     8041 tcggtcaggc cgtggtcatg atcgcgagta gtgggaaaaa cggccgacga gttagcggcg
     8101 gctacgttag ctccttggca ggttacgacg gtgaatggga atacatgatc gacaaatcga
     8161 tccgcgtcac cgcgttcaat cctggtttcg gcggcggcac gttggccaac tttcacggcg
     8221 agctggccgg cgttgtatcg ctgaatttga acgaggtcgg caaattcacg atggccatcc
     8281 ccgtggaata ctatcaaacc tacgagcaag aactgaaaca acatggccag gtgcgcagcc
     8341 ggccacgccg tccttggcta ggaatttaca cgcagggtgt cgccggtcta gtggtcatcg
     8401 gaaatgtcac ggggaatgga ccggcggcca aatgcggcct caaggccggc gatgtgattt
     8461 tatctgtcga aaacaacaag atccgttccc gccaggagct ttaccacgaa gtgtggaaaa
//
LOCUS       DATLCH010000013         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1532890, whole genome shotgun sequence.
ACCESSION   DATLCH010000013 DATLCH010000000
VERSION     DATLCH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532890"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..386)
                     /locus_tag="VMZ02_00380"
     CDS             complement(69..386)
                     /locus_tag="VMZ02_00380"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HUR70406.1"
                     /translation="MNTLAGSMAVPNRLPKLSPTYSNDIAEHCELRSITMSGLGRALI
                     SFGIVLVVVGALVSLSDKLPWLGHLPGDIHIQRGRFSFYFPLTTCVIVSIVISLIAYW
                     FKR"
     gene            complement(316..1341)
                     /gene="ruvB"
                     /locus_tag="VMZ02_00385"
     CDS             complement(316..1341)
                     /gene="ruvB"
                     /locus_tag="VMZ02_00385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HUR70407.1"
                     /translation="MLDGHRTDEDSGFELSLRPRGFDEYVGQDRVKENLKVAIAAARA
                     RGDVLDHILFHGPPGLGKTSIAYIIAREMDVNIKATSGPIIERAGDLAALLTNLDHGD
                     VLFIDEIHRLNHVVEEVLYPAMEDYEIDIMVGQGPAAKSIKLPLKKFTLIGATTRSGL
                     LTSPLRDRFGQSFRLDFYDSLSLARILKRSAAILSVEQDEDGIAEIASRSRGTPRIAN
                     RLLRRVRDYAQVKSEGKITKPVSVEALRMLEVDRLGLDKMDTMLLLTLIDKFSGGPVG
                     VETLAAAIGEEKDTIEDVYEPFLIQAGFIQRTPRGRVATVLAYEHFGRQHGGTKPAAK
                     AQPDLFE"
     gene            complement(1504..2100)
                     /gene="ruvA"
                     /locus_tag="VMZ02_00390"
     CDS             complement(1504..2100)
                     /gene="ruvA"
                     /locus_tag="VMZ02_00390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HUR70408.1"
                     /translation="MIAQISGKLAQKLPGEVVIEVGGIGYLVLIPLNVFYHLPDIGAP
                     MSLQIHTHVREDALQLFGFRDLAEKQVFLLLMGVSGIGPKLALNILSGIPAEDLARAL
                     RNGDQIRLVSIPGVGRKLAERMIVELKDKFTNLTPVTLDNTSKPEASSQKMQDAVSAL
                     INLGYKKPEIEKTVRDVLKNRELSLEDVLRETLRQMGH"
     gene            complement(2362..>2868)
                     /gene="ruvC"
                     /locus_tag="VMZ02_00395"
     CDS             complement(2362..>2868)
                     /gene="ruvC"
                     /locus_tag="VMZ02_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HUR70409.1"
                     /translation="ATGWGVVQIIGPALRHMGHGTIHSSSAEPQARRLCRIYTELSKV
                     VAEFRPDGVSVEKVFFAQNVQSALKLGQARGVALLAAAEKDVEVHEYSAVEIKSAVVG
                     YGHATKQQVQIMIGSLLKVSGPVPADAADALAAAICHLNRQAFASRVAEAMPRAPTPL
                     RPRSNGGR"
BASE COUNT          637 a          832 c          777 g          622 t
ORIGIN      
        1 gtggctaggg gttaagcttg gtgattccat gggcacggct gaatcgaaag cagatcaact
       61 gaaagtttct aacgtttgaa ccaataagca atgagcgaaa tcacgatgct gacgatgacg
      121 caggtggtca gtgggaaata aaagctgaag cgaccgcgtt gaatatggat atcgcccggc
      181 aaatggccga gccatggcaa cttgtcagac aacgacacca gtgctcccac gaccacaagc
      241 acaatgccaa aagagatgag cgccctgccc aatccactca tggtaatcga tctaagttca
      301 cagtgctccg caatgtcatt cgaataggtc gggctgagct ttggcagccg gtttggtacc
      361 gccatgctgc cggccaaagt gttcatacgc tagcacggtg gcaacccggc cgcgcggcgt
      421 ccgctggata aacccggctt gaattagaaa cggctcgtag acatcctcaa tggtatcctt
      481 ctcttcgccg atggccgccg ccaaagtctc cacacccacc ggtccgccgg aaaacttatc
      541 gatcaacgtc agcagcaaca tcgtatccat cttgtcaagc ccgagacggt caacctcaag
      601 catgcgcaac gcctccacgc tgaccggctt ggtgatcttg ccttcgcttt tcacctgcgc
      661 atagtcgcgc acccggcgca gcaggcgatt ggcaatgcgc ggcgtgcccc gtgagcggct
      721 ggcgatctcg gcgatgccat cctcgtcctg ttcgacgctc aggatcgccg cggagcgctt
      781 caggatacgc gccaacgaga gcgagtcgta aaagtcgagc cggaaactct gaccgaagcg
      841 atcgcgcaac ggcgatgtca acaaacccga ccgcgtggtg gcgccgatca aggtaaattt
      901 tttcaatggc agcttgatcg atttagccgc cgggccctgt ccgaccatga tgtctatttc
      961 atagtcctcc atggcgggat agagcacctc ttcgacgacg tgattgagac gatggatttc
     1021 gtcaatgaaa agaacatcgc cgtgatcgag attggttaaa agcgccgcca gatcgccggc
     1081 gcgctcgatg atgggtcccg aagttgcctt gatgttgacg tccatctcgc gcgcaatgat
     1141 ataggcaatg gaggtttttc cgagcccggg cgggccatga aatagaatat gatcgagcac
     1201 gtcgccgcgt gcccgcgcgg ccgcgatggc gactttaagg ttttccttga cgcgatcttg
     1261 gccgacgtat tcgtcaaagc cgcgcggccg caggctcaat tcgaatccgg aatcttcgtc
     1321 ggtgcgatgg ccgtccaaca tggtcagtgt atgcccttca acaacacagc ccgggcgggt
     1381 ttcaaacccg gccctctaca atcgtaattc ttttttggct tctttgcgcc ctttgcgtaa
     1441 atcacccgaa ttgattctgc ggcgcggtga tctccgtgcc ttggtagtaa aactcttctc
     1501 gtgctaatgg cccatctgcc gcagggtctc gcgcaggaca tcctccagtg acagctcacg
     1561 gtttttaagc acgtcgcgga cagttttttc aatctccggt tttttatagc ccagattaat
     1621 caaggcggaa accgcatcct gcatcttttg cgatgaagcc tcaggcttgc tggtgttatc
     1681 aagcgtaacc ggggtgaggt ttgtaaactt gtcctttagc tcgacgatca tccgctcggc
     1741 caacttcctg ccaaccccag gaatcgatac caagcgtatc tggtcaccgt tgcgcaatgc
     1801 ccgcgccaga tcttcggccg ggataccgga caaaatattg agcgccaact taggaccgat
     1861 accggacacg cccatgagca gcaaaaaaac ctgcttttca gcaaggtcgc gaaagccgaa
     1921 taactgtaaa gcgtcttcgc gcacatgagt atgaatttgt aaactcatcg gcgcaccgat
     1981 gtccggcaag tggtaaaaga cattaagcgg aataagaacc agatagccga tgccgccgac
     2041 ctcaatgacc acttcgccag gcaacttttg tgcgagcttg ccgctgattt gtgcgatcac
     2101 gtcgagcctt gcaatcgagt tacccgaccc tcggcttgaa gtcgagggtt tagtttggcg
     2161 ccgactttag atagaaattt tgctctgtcg ttcgggcgca aggccgaatc catcctcttt
     2221 gtcgtcactt gatgtgagac aaaaccgctg ccggcgttcg cggagaggat gaatctgcgt
     2281 tgcggcggaa ttaattcctg agtcatagcg aggggatttc atatcccgaa gtgggacagc
     2341 aaagctacct gcataagccg attatcggcc gccgttgctg cgcggtcgca aaggtgtcgg
     2401 cgccctgggc attgcttcgg ccacccggct ggcgaacgcc tgacgattga gatggcagat
     2461 cgccgccgcc aacgcatccg cggcatcggc cggcaccggc ccggaaactt tcaatagcga
     2521 tccgatcatg atttgcactt gctgcttggt cgcatggccg tagcctacca ccgccgattt
     2581 gatctccacc gcgctatact cgtgaacttc gacatctttt tccgcggccg cgagcagcgc
     2641 aacgccgcgc gcctggccaa gcttcagggc actttgaacg ttttgagcaa agaacacttt
     2701 ttcgacactc acgccgtccg gacggaactc cgctacgact ttcgacagct cggtataaat
     2761 ccggcatagg cgacgcgcct gaggctcggc gctcgaggaa tggatcgtgc catggcccat
     2821 atggcgcagc gcggggccga taatctggac aaccccccag ccggtcgc
//
LOCUS       DATLCH010000014         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5262161, whole genome shotgun sequence.
ACCESSION   DATLCH010000014 DATLCH010000000
VERSION     DATLCH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5262161"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VMZ02_00400"
     CDS             complement(<1..457)
                     /locus_tag="VMZ02_00400"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70410.1"
                     /translation="MDLRIAAVDLVSNTCFPALAADELGYFKNEGLDARIELAAMLGA
                     TKALRQGSADAMIAGSVFDLLTEFPNWHGAKIAVALSQGTPWLLVVRSDLAAKRGDLN
                     ALRGLRITAAEGPDLVLKHLLKCAGLEVGRDIEIVQLPGAKSRDVSFGVF"
     gene            641..1360
                     /locus_tag="VMZ02_00405"
     CDS             641..1360
                     /locus_tag="VMZ02_00405"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70411.1"
                     /translation="MPTPRNITLGLLSPATPNKPHFKHVDALLPRDVSLVHEGLGLLG
                     EAYQDLAGKADVVIVKARDFVARNKVDGLVLTGGFIGLFNPGIDVKVRDAIQLPVATA
                     VSSAVAALNIYKAKSVLLMTPFDAESDAVIEDHLKHLGFTVHLGPAFEERKAGADVDM
                     TPDELFDLAVKTYGSYDAADAIYFQGATMNPLPIIQRLENELKVPVVASNPAMFWNVL
                     SKLGVKDSVKGYGRLLEDWPA"
     gene            1505..2509
                     /locus_tag="VMZ02_00410"
     CDS             1505..2509
                     /locus_tag="VMZ02_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70412.1"
                     /translation="MQSFLAIIPRYPWLIAVILSLTSPLSAADKIRVGFSAVSMANAP
                     VWIAEEKGLFKKYGIETEPIVIGGGATRSVSAVIAGDLQFGSTGGGAVVSAVFSGSDI
                     VMVAAGNNKGIQRVMVKPEITTPAGLKGKRIGITTLGSSGHLALLLMLRKWNMVPEDV
                     QIIQVGASPVMFISLQKGGIDAAVLQDPTFFVAEDSGFKTLGDPVAMDIQYLQNVLVA
                     SRAYLKSHRELASRFMKGFVEGVAYFKRNKEDSMRILMKKMRVEKGKETYLERSYNLF
                     ATQYLEAAPYPSVVGTRTVLEFMAKDFPKAKTADPNSFIDNSLIKPLDDSGFIKGLYQ
                     "
     gene            complement(2679..>3101)
                     /locus_tag="VMZ02_00415"
     CDS             complement(2679..>3101)
                     /locus_tag="VMZ02_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70413.1"
                     /translation="GGVLDRFPTLKFGFFEGGIGWVPWLMHTLDAHMPNDATKSVAVQ
                     QFFEGKQAIKKQPSQYFDQFFIACVSWESYIADTVNGWPNHNIIIGSDFDHGDAVATW
                     PRTVDVIKQMKALSAADKEKILGGNAMKMFGLNGNGAH"
BASE COUNT          731 a          845 c          852 g          673 t
ORIGIN      
        1 caaaaacacc gaaagaaaca tcgcgcgatt tcgcacccgg caattgaaca atttcgatgt
       61 cgcggccaac ttccagcccg gcgcatttaa gcaaatgctt cagtacaaga tccggacctt
      121 cggcggcagt gatgcgcaac cctctgagcg cattcaaatc gccacgctta gccgccaaat
      181 cgcttcgcac cacgagcagc catggcgttc cctgtgacag tgcaacggcg attttcgctc
      241 cgtgccaatt ggggaactcg gtgagcagat cgaaaaccga cccggcaatc attgcgtccg
      301 cactgccttg gcgcagcgcc ttggtcgccc ccaacattgc ggccagctcg atgcgcgcat
      361 ccaaaccttc attcttgaag taacctaatt catccgccgc caaggcagga aaacaagtat
      421 tactcaccaa atcgaccgca gcgatgcgta aatccacggg aacctccgag ggaacagatt
      481 gtgaacctcg actatgcgag atcgatatgc gcgggtcaat cgactgacga gaaaaaccat
      541 aagtcattac gggtcagcgg taacgcgagt cggccggacc gaacgtttca cgatttagga
      601 taagttatga tatatgcaaa cggattagtt ggagcctttc atgccaaccc ctcgcaatat
      661 cactctcggc ctgctctcgc cggcgacgcc gaataagccg cacttcaaac acgtcgacgc
      721 gctcttgccg cgcgacgtgt cgttggttca cgagggttta ggtttgttgg gcgaggcata
      781 tcaggatctc gccggcaagg ccgatgtggt aatcgtcaaa gcgagagatt tcgtcgcgcg
      841 caacaaagtt gacggtttgg tactcacggg tggcttcatc ggcctattca atcccggcat
      901 cgacgtcaaa gtccgcgacg cgatccaatt gccggtggcc actgcggtgt cgtcggcggt
      961 cgcggcgttg aacatctaca aagccaaatc ggtgctgttg atgacgccgt tcgacgccga
     1021 gtcggatgcg gtgatcgaag atcatctcaa acacctcggc ttcacggtcc atctcggtcc
     1081 cgcgttcgaa gaacgcaagg cgggtgcgga cgtcgatatg acgccggacg agctgttcga
     1141 tctggccgtg aaaacatatg gcagttatga tgccgccgat gcgatctatt tccaaggcgc
     1201 gaccatgaat cccctgccga tcatccaacg cttggaaaat gaattgaagg tgcctgtggt
     1261 cgcgagcaac ccggcgatgt tttggaatgt cttgtcgaag ctaggtgtga aagattcggt
     1321 aaagggatac ggcaggctgc tcgaagactg gcccgcatag atatcatcgc atcgatcgct
     1381 aattatcacg gtcaaagtcc gtcatgccgg tgaaaaccgg catccaggtt gggtttggtg
     1441 gaaacgaaac ccatgatgga ttccggcgtc cgccggaatg acagagaatc gggagaatag
     1501 aaaaatgcaa agcttcctcg caataattcc acgataccct tggctgatcg cggtaatctt
     1561 gtcgctcacg tcaccgttgt ccgccgcaga taaaattcgc gtcggcttct ccgctgtaag
     1621 catggccaat gcacccgtgt ggatcgccga ggagaaaggg ttattcaaga aatatggcat
     1681 cgaaacggag ccgatcgtga tcggcggggg cgccaccagg tcggtgagcg cggtgatcgc
     1741 cggtgatctg cagttcggct caaccggcgg cggcgcggtg gtcagcgcgg tttttagcgg
     1801 gtcggatatc gtcatggtcg ccgcgggcaa caacaaaggt attcagcggg tgatggtcaa
     1861 gccggagatt accacgccgg ccggcttgaa ggggaagcgg atcggtatca cgacattggg
     1921 ctcatccggc catctcgcct tgctcctgat gctgcgcaaa tggaacatgg ttccggaaga
     1981 cgtgcagatc attcaagtcg gcgcgtcgcc ggtgatgttc atcagcctgc aaaagggcgg
     2041 tatcgacgcg gcggtgttgc aagacccgac cttttttgtc gccgaagaca gcggctttaa
     2101 aaccctcggt gatccggtgg cgatggatat tcaatacctg caaaatgtct tggtcgcgtc
     2161 ccgcgcctac ctgaaaagtc accgcgaact cgccagccgc ttcatgaagg gtttcgttga
     2221 aggggtggcc tacttcaaac gtaataaaga agacagcatg cgcatcctca tgaaaaagat
     2281 gcgcgtcgaa aaaggcaaag agacttatct cgagcgctct tacaatctgt ttgccactca
     2341 gtacttggaa gctgcgccct acccttcggt cgtcggcacc cgaacggtgc tcgaattcat
     2401 ggcgaaggat ttcccgaaag ctaagacggc cgatccaaat tcgtttatcg acaatagttt
     2461 gatcaagccc ttggatgata gcggattcat caaaggcctg tatcaataaa caggcatcga
     2521 caatcctgat ttcggcgtgc cggtgaaaac cggcatccat cttgatattg gctgcagctg
     2581 aataaatccg gattccggcg ttcgccggaa tgacgaaatc aacaagtgat cttgagaagg
     2641 tgagccccta attcctcgtt atcttaattg acaacttctc agtgagcccc gttgccgttc
     2701 agtccgaaca tcttcatcgc gttgccgccc aatattttct ctttatcggc agccgacaac
     2761 gctttcatct gcttgatcac gtccaccgtg cgcggccaag tagccacggc gtcgccatgg
     2821 tcgaagtcac tgccgataat aatgttgtgg ttgggccagc cgttgacggt gtcggcgata
     2881 tacgactccc atgaaacgca ggcgatgaaa aactgatcga aatactggct cggctgtttc
     2941 ttgatggctt gtttgccttc gaagaactgc tgcaccgcaa ctgatttcgt cgcgtcgttg
     3001 ggcatgtgcg catcgagcgt gtgcatgagc cagggcaccc agccgatacc gccttcgaag
     3061 aagccgaatt tcaacgttgg aaaacgatcg agtacgccgc c
//
LOCUS       DATLCH010000015         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8983784, whole genome shotgun sequence.
ACCESSION   DATLCH010000015 DATLCH010000000
VERSION     DATLCH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8983784"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(890..1441)
                     /locus_tag="VMZ02_00420"
     CDS             complement(890..1441)
                     /locus_tag="VMZ02_00420"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HUR70414.1"
                     /translation="MAGKTNEFGFVECADLINGQKYGAALKTFQQAVANFLDSKTSPK
                     RNEIARQLFQIGSSLEETLRNAFGDKWESQIPQFADDSKLLTCSFCGKNQDEVGKLIA
                     GPSVHICSECVRTCDEILTKENVDGIEAGGETAASLTEERLCGICMEARETDELIFLP
                     HAAYMCAGCLEEIQLVRDKRQAP"
     gene            complement(1567..1953)
                     /locus_tag="VMZ02_00425"
     CDS             complement(1567..1953)
                     /locus_tag="VMZ02_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70415.1"
                     /translation="MRKITNGIVLMLIVVVFEIRLAGLLPAADFNTFLLAFKAAVARN
                     DAKAVAGMTQFPFLFDSRTRDSSDFQKIYPQLFDANVRTCFAKAKPVIEQDAQVVNCG
                     RYIFYFRVVKDRYRFIEFTADPEAVQ"
     gene            complement(2040..2789)
                     /locus_tag="VMZ02_00430"
     CDS             complement(2040..2789)
                     /locus_tag="VMZ02_00430"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR70416.1"
                     /translation="MKLTNKVAVVTGAAQGIGRATALMMGREGAKLVVADLQSEKAQS
                     VANELQAQGAEAIALEVNVANESSVKRMAKSTFERFGRADILVNVAGIYFPKASVVDL
                     SEEDWDRTINVNVGSNFLCCREFVPAMRAQKSGRIISLASGIGHYGMRQFSHYAASKA
                     AVMGFVKALAREVGPDGITVNAICPGSANTSMPRQHRSEEEVMARLTATPLPHILEPE
                     DIAGSIVFLASDAAKFITGQSYNVNSGTYMF"
     gene            complement(2786..>3116)
                     /locus_tag="VMZ02_00435"
     CDS             complement(2786..>3116)
                     /locus_tag="VMZ02_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR70417.1"
                     /translation="SNYGVTGSALRTPYAAAKAGIITFTKSLALELAPDLVLANTVAP
                     GPTDTPRVMEKESPEARRERWWSQIPLGRTALPEDLAEMYYFLVTPESAAITGQTFHV
                     NGGVVMP"
BASE COUNT          708 a          867 c          826 g          715 t
ORIGIN      
        1 tgaaggcatc gattacgaga tcaagcgcaa gcttggtcgt gacaacgcag cgaagctcta
       61 cggcttgatg acctaaccac aaatccagag atcgagagat tagactttcg atgccagaag
      121 ttgacagcgc catagatttc atagcgcgct gcaaaacctt tttccgaata ctttggtaac
      181 actgatcgaa gcaaccattc cgacacaggt gtctcaaaaa agctccacta aaatggctgg
      241 acgagaaatt caacattgtc gagtagtgta aaggtcgatt taacttataa aatacagcaa
      301 taaaaaccgc aacgaaattg ctgctatggc acaatgattg ctcgctttat ccgacgaaaa
      361 cacgcagtcg tgtgcgcttg aaatttattt ccaggtaaac agagtgttgc cgcggttaaa
      421 ccatactgac gagtcatatc gccaccgaga gcccggccaa gtcgagccaa aaacctcgac
      481 cctatcgcaa gagcctgtcg aagacctggt cagcccaccg gcaattgacg ctaaccctaa
      541 aacatttcga ccgccgtggc gccacccgca tcttatttgt cccaattgca aagctggaga
      601 ggtcaatcgg gtgggccgcc gtggcctcgg tgatcacctg cgcagcatga tttacattta
      661 tccattcaga tgcgaggcgt gcggtcactg ctttcgcgta tttgaatggg gcgtgcgcta
      721 ccgccgacag cggcgcggct gaaagtcgca gccgccgtct ttcgaaaagt tccccgaccg
      781 gtttccgtac gacagtggtg ctgcgacccg gtgattaccg tccacctcta tacgacgatt
      841 ctggatatct ttgcagtgaa ttcgtaaccg catgacgccg acgcaaaaac tagggcgcct
      901 ggcgcttgtc gcgcacgagc tgaatctctt ccaaacagcc ggcgcacata taggctgcgt
      961 gcggcaaaaa gattagctcg tcggtctcgc gtgcttccat gcatatgccg cataggcgtt
     1021 cttctgtgag actggcagcg gtttcccctc ccgcttcgat tccgtcgaca ttctcctttg
     1081 taagaatttc gtcgcaggta cgcacacact cgctgcagat atgcaccgag ggtccggcaa
     1141 tcagtttgcc cacttcgtct tggttcttgc cgcaaaagga acaggtgagc agtttactgt
     1201 cgtcggcgaa ttgcggaatt tgcgattccc atttgtcgcc gaaggcattc cttaaggttt
     1261 cctccaggct cgaaccgatc tgaaacaatt gtcgcgcgat ctcgttgcgc ttgggtgagg
     1321 ttttggaatc gaggaaattt gccacggctt gctggaatgt tttcaacgcc gcgccgtatt
     1381 tctgtccgtt gatcaggtca gcgcactcga cgaatccaaa ttcgttggtt tttccagcca
     1441 taaccatccc cttccgcccg ctgtttccgt cgcatttatg tcacatttcg accgttggtg
     1501 gcgcgaccgt agcacaattt tcattctggg acagcaaaac cgatttgcag tttaaatatt
     1561 cccgagtcac tgaaccgctt ccggatcggc ggtgaactca ataaaacggt agcgatcttt
     1621 cacaactcgg aaataaaaga tgtaacggcc gcagttgacg acctgagcat cctgctcgat
     1681 caccggcttt gccttggcga aacaagtacg aacattggca tcgaacagct gcggatatat
     1741 tttttggaaa tcagacgagt cgcgcgttct gctatcgaag agaaacggga actgggtcat
     1801 gccggcgact gctttggcat cgttacgtgc gacagcagcc ttgaaggcaa gcaaaaacgt
     1861 attgaaatca gccgccggta acaggcccgc tagcctaatc tcaaagacca cgactatcag
     1921 catcaggacg attccgttcg tgatcttgcg caatgcaact ctcccccgtg ttatgcagcc
     1981 gaatacgact ctgaaaggcg tagcgctgcg ccagtaacct cggcagtgag tgtgcgagat
     2041 caaaacatat acgtaccgct gttgacgttg tacgactgtc cggtgatgaa cttggcggcg
     2101 tcactagcaa ggaagacgat cgagccggcg atatcttcag gttcgagtat atgcggcagc
     2161 ggtgtggcgg tcaggcgagc cataacttct tcttcggagc ggtgttggcg cggcatggaa
     2221 gtattggccg agccgggaca gatggcgttt acggtgatgc cgtccgggcc gacctcgcgg
     2281 gctagtgctt tgacgaatcc cataaccgcg gctttcgatg cggcgtaatg cgagaactgg
     2341 cgcatgccgt agtggccgat gcccgaagcc aaggaaatga tcctgccgct tttttgcgcg
     2401 cgcatcgcgg gcacgaactc gcggcagcag agaaaattgc tgccgacgtt tacgttaatc
     2461 gtgcgatccc aatcttcctc agacaagtcg actaccgatg ctttaggaaa gtaaatccct
     2521 gcgacgttga ccaaaatatc ggcgcggccg aagcgctcga aggtgctttt tgccatgcgc
     2581 ttgaccgaac tctcattggc gacgttcact tccagtgcaa tcgcttcggc accctgcgcc
     2641 tgcaactcat tggccaccga ctgagctttt tcagactgta agtccgcgac gacgagcttg
     2701 gcaccttcgc gtcccatcat tagcgcggtt gccctgccga taccctgcgc ggcaccggta
     2761 acgacggcga ctttgttcgt gagtttcatg gcatcaccac cccgccgtta acatgaaatg
     2821 tctggccggt tatcgcggcg ctctccggag tgactagaaa gtaatacatc tccgccaagt
     2881 cttccggcag cgccgtgcgc ccgagcggaa tctgtgacca ccagcgctcc ctgcgcgcct
     2941 cgggcgattc tttttccatg acccgcgggg tatccgtcgg accgggcgcc acggtgttgg
     3001 cgagcacgag atctggcgcc aattccagag ccagcgactt ggtgaaggtg atgattcctg
     3061 ccttggccgc ggcgtagggg gtgcgcaatg ccgaaccggt aacaccgtag ttcgac
//
LOCUS       DATLCH010000016         3553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_46270, whole genome shotgun sequence.
ACCESSION   DATLCH010000016 DATLCH010000000
VERSION     DATLCH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46270"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="VMZ02_00440"
     CDS             complement(<1..1758)
                     /locus_tag="VMZ02_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HUR70418.1"
                     /translation="MVSVVVCLVGMMILSGFRLWLYRERRSDVGVLSVREKVKLFWIG
                     MRLDGVIVSRACMPFLVAILVLPDSLLLASRFVFALYLGIFYFTLFLAEIAGIYFFRF
                     YDFRPNYLIFEHGADREVLKTVAKAYPLVRIILLSLAGAWLATIAVQLLAPTARVNAQ
                     GENSWWLWDRALAFLWLGLAAVASRGTFDHRPLNPSLAAFTTNRIANEIAGCGIFNLL
                     YEWSQRAKNEFVALKSVTQVPSVDEAFADARRILSRDGRFCDDGPNPLTRRIANPSAP
                     APLNVVLIVMESFTGRLTGCLGGSPGLSPHLDMLAEEGLLLERCYATGERTIQGLEAA
                     VCSFPPLPGEGVVKRPQARQNFATIASVLKQRDYATTFLYGGQGIFDHMLGFFLGNGF
                     DRFIEEKDFPAPRYKSPWGVSDEDLFERADAEFRRYHENGQPFFATLLTVSLHSPWQF
                     PAGKIEPLPADSKVPAGFELDELNNFLYADYCIGQFIRLARQAPYFDNTLFVFVGDHG
                     VHLRGNQLIPIDDYVVPALFLAPKHVDSGRIAAVASQMDLPPTIMGIVGGEYRSAFLG
                     RDVRKPTADDPFALVIYNKK"
     gene            complement(1803..3203)
                     /locus_tag="VMZ02_00445"
     CDS             complement(1803..3203)
                     /locus_tag="VMZ02_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70419.1"
                     /translation="MNVLGLRGAYRTISQFHENPRLVAWLSAQPWVIGFTAERRKVVL
                     FFGAIIAGVAGVFSRGATWRDYRALTPWLEHGLAFLFLLGLLYALYLAVIHFKQLPVT
                     VRRRPQIWFHLLFWLLLVCLWFFTERGGFAVAVLALILLSFPYLIWRAGYMLLSGQRG
                     KAFNSRFRDHLFYIWPIWDGTNTPPGKGHDYLSRTQAQSPEAYARAILGGCKLLLLVA
                     IWKICLELLGALIYGDPKSSFSALLRGYSLGLPRLKQILGARMLEISLLQVWLSLYLE
                     LIWETLSLAAKGHVWVGVIRLFGFNIFRNTYKPLLARTIVEFWNRYYYYFKELMMECF
                     FLPTYARHFRQWPRLRVIAAVFAAAFVGNMYYHLLQHKEPLVLGDWNKLWSLLGARLV
                     YCAMLAVGIAISMLRQQRQRSQVQAIVDGQPNRFFTLRAIAGVWTFFAVINFWNVTSH
                     LTIAGRARLFFSLFGF"
     gene            complement(3200..3460)
                     /locus_tag="VMZ02_00450"
     CDS             complement(3200..3460)
                     /locus_tag="VMZ02_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUR70420.1"
                     /translation="MAERERQRLELIEFLRTIQRPDYPLDGIDEESSLVESGLIDSLA
                     LLQIVTYLEQTYHIDFNERGIDPEDLRSVNSILELIARDSAS"
BASE COUNT          956 a          989 c          951 g          657 t
ORIGIN      
        1 ctttttgtta taaatcacca gagcgaacgg atcatcggct gttggtttgc gaacatctcg
       61 ccctaaaaat gcgctgcgat attccccgcc gacgattccc atgattgttg gcggtaaatc
      121 catctggctc gctacagcgg caatacgccc gctatcgaca tgtttcggcg cgagaaacag
      181 cgccgggacg acataatcgt cgatcggaat cagttggttg ccgcgcaagt gcacgccatg
      241 gtcgccgaca aagacaaaca gcgtattgtc gaaatacggc gcttgccgag ccaagcgaat
      301 gaattggccg atgcagtaat ccgcgtaaag aaagttattg agttcatcga gctcaaagcc
      361 cgccggcact ttgctatcgg cgggcaaagg ctcgatcttt cctgcaggaa actgccaggg
      421 cgaatgcagc gacaccgtca gcaaagtagc aaagaatggt tgaccgtttt catgatagcg
      481 acggaactcc gcatcggcac gttcgaacaa atcttcgtcg gatacgcccc aggggctttt
      541 gtagcgcggc gctggaaaat ctttttcctc aatgaagcgg tcaaagccgt tcccgagaaa
      601 aaaaccaagc atgtgatcga aaatcccctg cccgccataa agaaacgtcg tcgcatagtc
      661 gcgctgtttg agtacactcg cgatcgtagc gaagttttgc cgcgcttggg gtcgtttgac
      721 aacgccttcg ccgggcagcg gcggaaaaga gcaaaccgcc gcctccagtc cttgaatcgt
      781 tcgctctccg gtggcgtagc aacgttccaa taaaagacct tcttcggcca gcatatctaa
      841 gtgaggcgag agcccggggc tgccgccaag acagccagtc agccgaccgg taaaactctc
      901 catcactatt agcaccacat tgagtggtgc cggcgctgaa ggattggcga ttcggcgcgt
      961 tagtggattg gggccatcgt cacagaatcg accatcccgc gatagaatcc gtcgcgcatc
     1021 ggcaaacgct tcgtcgacgg acggcacttg ggttacgctc tttagggcca cgaattcgtt
     1081 ttttgcacgc tgactccact cgtagagcag attgaaaatc ccacaaccgg caatctcatt
     1141 ggcgatccga ttggtggtaa acgcggcgag gctcggatta agcggacggt gatcaaatgt
     1201 accgcggcta gcgacagccg cgagcccaag ccaaaggaat gccagggcgc gatcccacag
     1261 ccaccacgaa ttttctccct gagcgttcac acgtgcggta ggcgcgagca actgcacggc
     1321 aatcgtcgcc agccaagcgc cagcgaggct tagcaagatg atgcgcacga gaggataggc
     1381 tttggcgacg gtcttgagaa cttcacgatc cgcgccgtgt tcgaaaatca gataattggg
     1441 acggaaatca tagaagcgaa agaaataaat gccggcgatc tcggcgagaa atagcgtaaa
     1501 gtagaagatc ccgaggtaca gggcaaagac aaatcgactc gcaagcagca aggaatccgg
     1561 caacaccagt atggctacta ggaaaggcat gcaggcccgg ctgacaatca cgccgtcaag
     1621 ccgcataccg atccagaaca acttcacctt ttcgcggacg ctgaggacac ccacgtccga
     1681 gcgccgttcg cgatagagcc ataagcgaaa acccgacaag atcatcatgc cgaccagaca
     1741 aacaacgaca gagaccatgg tgcgcggcag tgcagtcatt gtatggtgcg gcctaggcca
     1801 gattagaagc caaacagtga aaaaaacaat cttgcacggc cggcgatggt gagatgggac
     1861 gtaacgttcc agaagttgat gaccgcgaag aaagtccaaa ccccagcaat ggcgcgcaga
     1921 gtgaaaaacc ggttgggttg tccgtctacg attgcttgca cctggctgcg ctggcgctgc
     1981 tggcgcagca tggagatcgc gattcccaca gcaagcatag cgcaataaac caaacgcgcg
     2041 ccgagtagcg accagagttt gttccagtcg cctaaaacca gcggctcttt atgttgcaac
     2101 agatgatagt acatgttgcc gacaaaggcc gcggcgaaaa ccgcggcgat gactctcaac
     2161 cgtggccatt gacgaaagtg gcgcgcatag gttggcagaa aaaaacactc catcatcaat
     2221 tctttaaagt agtaataata acggttccag aactcgacaa tggtccgtgc caacagcggc
     2281 ttgtaggtat tgcgaaatat gttaaagcca aacagccgga tcacccccac ccaaacatgg
     2341 cccttggccg ccagcgaaag ggtttcccag atcaactcca gataaaggct gagccatacc
     2401 tgtagcaagg atatttccag cattcgcgca ccgaggatct gcttcagccg cggcaaaccg
     2461 agagagtaac cgcgcaatag cgcgctgaaa gaactcttcg gatcgccgta aatcagcgcc
     2521 ccgagcagtt ccaaacaaat cttccagatt gcgaccagga gcaagagttt acagccgccg
     2581 agaatggcgc gcgcataggc ttcaggtgat tgcgcctgcg tgcgcgagag ataatcatgg
     2641 cctttgccgg gcggcgtatt ggtgccgtcc caaatcggcc aaatataaaa aagatgatcg
     2701 cgaaagcggc tgttgaacgc cttgccgcgc tgtcccgaca gaagcatata gccggcccgc
     2761 cagatcaaat atgggaacga taacagaatc agcgccagaa cagcgacggc gaaaccaccg
     2821 cgttcggtga aaaaccacag gcacaccaga agcagccaga acagtaaatg aaaccaaatc
     2881 tgcggccgtc gacgcaccgt caccggcagc tgcttgaaat ggatgacggc gagataaaga
     2941 gcgtacagca gaccgagcaa aaacagaaaa gccaaaccat gctcgagcca cggcgtcaaa
     3001 gcgcgatagt cgcgccacgt cgcgccgcgg ctgaagacgc ctgccacccc agcgattatc
     3061 gcgccgaaaa aaagcacaac cttacggcgt tccgcagtaa acccgatcac ccaaggctgg
     3121 gcgctcagcc aagcgacaag acgggggttt tcatggaatt gcgaaatggt ccggtaagcg
     3181 ccgcgcaggc cgaggacatt catgatgccg agtcgcgggc gattaattcg agaatagaat
     3241 tgaccgaacg cagatcctcc ggatcaatgc cacgttcatt gaagtcgatg tgataggtct
     3301 gctccaggta ggtgacaatt tgcaggagcg ccaacgaatc gatcaagcca gactccacca
     3361 agctcgattc ttcgtcgatg ccgtccagcg gatagtcggg tctctgtatt gtacgcaaga
     3421 attcaatcag ctctagcctc tgccgttcac gttctgccat ctcgtcttcc aatttcactt
     3481 acagtgtcta tctatcctag tcgttcggct catgagacgc gccgcaacaa tccaaccaaa
     3541 tccacgggct tta
//
LOCUS       DATLCH010000017         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5265288, whole genome shotgun sequence.
ACCESSION   DATLCH010000017 DATLCH010000000
VERSION     DATLCH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5265288"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="proB"
                     /locus_tag="VMZ02_00455"
     CDS             complement(<1..1044)
                     /gene="proB"
                     /locus_tag="VMZ02_00455"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HUR70421.1"
                     /translation="MTQLEHKQPILQRARRIVVKIGSQILSSQDGIEESRLRGLVRQL
                     AELHGGERQVVVVSSGAVAAGMTQLGWKGRPKTIPEKQALAAVGQIKLMALYERSFAK
                     YAKNVAQVLLTAEDLANRQRYINAKHTFQMLLESSIIPIVNENDTVAVEEMKFGDNDH
                     LSALVATLLEADLLVILSDVEGVYDRDPRVHDDAQLIHLISEARGLRHAIGGATVSAT
                     GTGGITTKLAAAEEAAAAGIPTIIASGMQNSVLEKIFDARNEIGTLILPELKHLRRKK
                     HWIGFNLRPVGTIVVNEGAYQAVADKGKSLLPAGVEKVEGSFGVGDCVRIDFHGKEFA
                     RGLVNYGKTELNRI"
     gene            complement(1025..2062)
                     /gene="obgE"
                     /locus_tag="VMZ02_00460"
     CDS             complement(1025..2062)
                     /gene="obgE"
                     /locus_tag="VMZ02_00460"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HUR70422.1"
                     /translation="MKFIDETKIRVIAGDGGPGSVSFHRAKFVRRGPADGGNGGHGGD
                     VVAVADPQLTTLLDLRYRKEWKASPGKRGGDADCDGRRGEDCIIKVPVGTLIRDLSGE
                     VLADLKFPGERVVIAAGGRGGKGNAHFASSTNRSPRFCQPGEPGVERELEIELRLLAD
                     VGIIGLPNAGKSTLISVISSVRPKIADYPFTTLVPNLGVVSYGEGKSFVAADIPGLIE
                     GAHQGHGLGDKFLRHILRTRVLVHVLDASQLDGKDPLAAWKAINREMELFDPELIDKP
                     QIVVANKVDLPEGRELAKVLDEKLPKAYRPLHVISAATTEGVQQLVYAIGRKLDEVKQ
                     QSETDRDAARA"
     gene            complement(2239..2496)
                     /gene="rpmA"
                     /locus_tag="VMZ02_00465"
     CDS             complement(2239..2496)
                     /gene="rpmA"
                     /locus_tag="VMZ02_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HUR70423.1"
                     /translation="MAHKKAGGSSRNGRDSNGQRRGVKVYGGETVRAGNILVRQLGTK
                     IHPGTNVGMGRDYTLFSLVDGVVKYGRYDKTRKKVEVVPAQ"
     gene            complement(2510..>2847)
                     /gene="rplU"
                     /locus_tag="VMZ02_00470"
     CDS             complement(2510..>2847)
                     /gene="rplU"
                     /locus_tag="VMZ02_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HUR70424.1"
                     /translation="ISFRYNSMKYAVIRTGGKQYRVSEGDVVKIEKLDAEVGETITLA
                     DVLFFGNDDDVKIGTPLVANAKVIGEIVDQSRAKKIIVFKKKRRKSYSRQQGHRQYQT
                     ALKITSIEA"
BASE COUNT          595 a          850 c          714 g          688 t
ORIGIN      
        1 gattcgattc aactcggttt taccgtagtt gaccaagccg cgggcgaatt ccttgccgtg
       61 aaagtcgatt cggacgcaat cgccgacccc gaacgatcct tcgacttttt cgacgccggc
      121 cggcagcagg ctcttaccct tatcggcaac agcctgatac gcgccttcat tgacaacgat
      181 agttcccacc ggcctgagat taaaaccgat ccagtgcttt ttacggcgca gatgtttcag
      241 ttccgggaga atcaatgtgc caatctcatt cctggcatcg aagattttct cgagcacgct
      301 gttttgcata ccactggcga tgattgtcgg gatgcccgcc gcggcagcct cttcggcagc
      361 tgcgagtttt gtcgtaatgc caccggtacc cgtcgcactg acagtggcac cgccgatcgc
      421 gtggcgcaag ccgcgcgcct cgctgatcaa gtgaatcagc tgagcatcgt catgcacccg
      481 cggatccctg tcgtagacac cctcgacgtc gctcaagatc accagcaagt cggcttcgag
      541 cagcgtcgct actaatgcgg atagatgatc gttgtcgcca aacttcattt cctcgacggc
      601 gacagtatcg ttctcgttga caatcggaat aatcgacgat tccagaagca tctggaacgt
      661 atgttttgcg ttgatgtagc gttggcggtt ggccagatcc tcggcagtca acaatacttg
      721 agcgacattt ttggcatatt tggcaaaact gcgctcgtaa agcgccatca atttgatttg
      781 tccgacggcc gccagcgctt gtttctcagg aattgttttc ggccgcccct tccaacccaa
      841 ctgagtcatt ccggccgcta ccgcgcctga actcacgacc acaacctggc gctcccctcc
      901 gtgtagctcc gcgagttggc gtaccaagcc gcgcaggcgc gactcctcaa tgccatcttg
      961 cgatgacagt atttggctgc cgatcttcac cacgatgcgg cgcgcccgct gtaagatagg
     1021 ctgtttatgc tctagctgcg tcacgatcgg tttcgctttg ttgcttcact tcgtcgagct
     1081 ttcggccgat cgcatataca agttgctgca cgccctcagt ggttgccgcc gaaattacat
     1141 gcaagggtcg ataggccttc ggtagttttt catccagtac cttggctaat tcccggcctt
     1201 ccggcaggtc tactttattg gcaactacga tttgcggctt gtcgatcagt tccgggtcga
     1261 aaagctccat ctcacggttg atcgccttcc atgccgctag agggtctttg ccgtcgagct
     1321 gcgaggcgtc aagaacatgg accaatacac gggtacgtaa aatgtgacga agaaatttat
     1381 cacccaagcc gtggccttga tgcgcccctt cgataagtcc cggaatatct gcggcgacaa
     1441 agctcttgcc ttcaccgtaa ctgaccacgc ccaaattagg caccagcgtg gtaaacggat
     1501 aatcagcgat cttcggccgc accgacgaga tcaccgagat caatgtcgac ttgccggcat
     1561 tgggtaaacc gatgatcccg acatccgcga gcagacgtag ctcaatttcg agctcgcgct
     1621 cgacgccagg ttcacccggt tgacagaaac gcgggctgcg attggtcgaa gacgcaaagt
     1681 gggcgttgcc tttgccgcca cgcccgccag cggcgatcac cactcgctcg ccaggaaatt
     1741 ttaggtccgc cagcacctcg ccgctgaggt cgcggatgag ggtccctacc ggaaccttga
     1801 ttatgcaatc ttcgccgcgt cggccgtcgc aatcggcgtc gccgccgcgc ttcccgggac
     1861 tcgctttcca ctccttgcga tagcgcaggt cgagcaaggt cgtgagctgc gggtccgcca
     1921 ccgctacgac atcgccacca tggccgccgt tgccgccatc ggcgggaccg cggcgtacga
     1981 atttagcacg gtggaaactc acgcttcccg gccccccgtc gccggcgatc acccgtattt
     2041 tggtttcatc gatgaatttc ataaagcagc cattagttaa gatatggctt ctagcatgac
     2101 ggatattgcc tcgctccgct agcctgacac cgccacgaaa aaattgcccg tttaatcagc
     2161 gaagcctatc ttcgccggca ttcagacaaa aaaaaagccc cgaaaggggg cttctttcat
     2221 cggagtccaa cgagtaaatt actgagccgg gacaacttca actttcttac gggttttgtc
     2281 ataacggcca tatttaacaa cgccatcgac aagagagaac agcgtgtagt cgcgccccat
     2341 gccgacattg gtccctggat gaatcttggt gccgagctga cgcactaaaa tattgccagc
     2401 ccgcacggtt tcgccgccgt aaactttgac gccccgccgt tgtccattgc tatcgcgccc
     2461 attgcgcgaa cttccgcctg cctttttatg tgccatattc gctcccgagt taagcttcga
     2521 tcgacgtgat cttcaaagcc gtctgatact gacggtggcc ctgttgccga ctgtagcttt
     2581 tgcgccgttt cttcttgaaa acaatgattt tcttggcgcg gctttgatcg actatttcgc
     2641 cgataacttt ggcgttagcc accagtggag tgccaatttt tacgtcgtcg tcgttgccaa
     2701 agaacaaaac atccgcgagt gtgattgtct cacccacttc ggcgtcgagc ttttcaatct
     2761 tgacgacatc accttcagag acgcggtatt gtttaccgcc ggttctaatt actgcgtatt
     2821 tcatagaatt ataccgaaac gagattt
//
LOCUS       DATLCH010000018         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_54720, whole genome shotgun sequence.
ACCESSION   DATLCH010000018 DATLCH010000000
VERSION     DATLCH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54720"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..929
                     /locus_tag="VMZ02_00475"
     CDS             210..929
                     /locus_tag="VMZ02_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HUR70425.1"
                     /translation="MLSVLAFAGCSSVKSPAPKASPRPVSNVFHVVKPGENLFRIGKA
                     YDISTEELARANQLRDPNQIYIGQRIYIPGANRQLPVDTIAPAEIAPTSRISPTGHAS
                     SEPLLWPVTGTINSTYGPRGASFHDGIDIAAPEGTPIQVIEGGEVIYSDQLRGYGNIV
                     IVRHQGGIVSVYAHNQINLVREGQQVGKGEVIARVGSTGRVTGPHLHLEIRQNNSAQD
                     PLRYLPQLCCIQASDSVSPKE"
     gene            942..1568
                     /locus_tag="VMZ02_00480"
     CDS             942..1568
                     /locus_tag="VMZ02_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70426.1"
                     /translation="MKELGKIRNDVVGSLLRPTKLKDARNQYDEGKLTLEQLREIEDQ
                     AIREAVKLQESIGLSVVTDGEYRRLNFQDSFGESVSGYDAGKPSLKFYESRVEGSSPL
                     RRWEIPDRGEQKGTAVAQRRPAVAKLGLTRNVPAAEFNFLRQVADRPGKVALIGPDRI
                     SQRFDWQNSKAIYSGLDEFVDDVVKVEREIINGLVQAGCLYVQIDAPG"
     gene            1578..2090
                     /locus_tag="VMZ02_00485"
     CDS             1578..2090
                     /locus_tag="VMZ02_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70427.1"
                     /translation="MDRPSLDAMRGRGEDPKENFSRSIRADNAVLAGFDDVTFGIHLC
                     RGNQRSMWHREGTYDEIAERLLNELNHDRFLFEYDTPRAGGFEPLRFLPKGKVIVLGL
                     VSTKVPQLETVDELKRRIDAAAKYTPLEQIAISPQCGFSSDVVGNLISDDDQRRKLEV
                     VVETARQVWG"
     gene            2155..2691
                     /locus_tag="VMZ02_00490"
     CDS             2155..2691
                     /locus_tag="VMZ02_00490"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HUR70428.1"
                     /translation="MGDDIARIRAAIRDIPDFPRPGIVFKDITPLLSNGPLFGKTVDL
                     IAERYRGQKVDTVLGIESRGFIFGSVLAYKLGAGFSVVRKPGKLPYETHSADYELEYG
                     KDTLEIHVDAVPRDARVVIADDLIATGGTAAATAELVSKLGATVVECAFVIELSFLHG
                     REKLKPHKVFSVLQYDSE"
     gene            2841..3410
                     /locus_tag="VMZ02_00495"
     CDS             2841..3410
                     /locus_tag="VMZ02_00495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HUR70429.1"
                     /translation="MKRIEQMTLWERLYFPEIFRGLMVTGGRFWRNLAIHALQKFGLA
                     KDTSAAMTVQYPEEKWVYPATYRGRHRLTLKPDETVQCTACYLCATACPAECIYIEAG
                     EYADKSVEKFPIRYEIDTLRCIYCGYCVEACPCDAIRMDTGTHPGNLGFSRKDFVETK
                     EVLMQRSKDLAEKGKEGLYEEYVKPYRHV"
BASE COUNT          831 a          922 c         1001 g          790 t
ORIGIN      
        1 atgcgcttga cgcggacccg cacgagtttc aaaaaggagc agttgggcga gtgtaagttc
       61 gtcaaattgc ttggcaagta cggctggcgc gaatagcgtc aaacaacgta atcgcactct
      121 tccggtgtga ctgtcttcga atgcgtctca agcccgtcaa agctggactc ccaggtgcca
      181 tgcgtttccg gttacaactg ctatggattg tgctgtctgt tctggcattc gccggttgtt
      241 cctcggtcaa gagtccggcg ccaaaagcgt caccgaggcc agtatcaaac gtttttcatg
      301 tcgtcaaacc gggcgagaat cttttccgca ttggtaaggc ttacgatatt tcgactgagg
      361 aattggcaag ggccaaccaa ttacgggatc ccaatcagat ctacattggt caaagaatat
      421 atattcccgg agccaatcga caactgccgg tggataccat cgctcctgcc gaaatagcgc
      481 cgacgtcgcg catctcaccg actgggcacg cctccagcga accattgctt tggccggtta
      541 ccggtacgat caattcaacc tacggtccac gcggagcgag tttccatgac ggtatcgata
      601 ttgcggcgcc ggaaggcacg ccgatccagg tcatcgaggg cggcgaagtg atttatagtg
      661 atcagcttcg cggctacggc aatatcgtca ttgtgcgcca tcaaggaggc atcgtttcag
      721 tatacgcgca taaccaaatc aatttagtgc gcgaaggaca gcaggtcggc aaaggcgagg
      781 tgattgcccg agttggcagc accggacgag tgaccggacc acatttacat ttggaaattc
      841 gccagaacaa cagcgcgcaa gatccgttgc gctatttgcc gcagttgtgc tgcatacagg
      901 cttccgatag tgtgtcgccg aaagaatagg ggatgtgggt catgaaagag ctcgggaaaa
      961 ttcgcaacga cgtcgtcggc agcttgctga ggcccacgaa gctgaaagat gcgcgcaacc
     1021 aatacgatga aggtaaactt acactcgaac agttgcgcga aattgaagat caagctattc
     1081 gcgaagcagt caagctgcaa gagtccatcg gattatccgt agtgactgac ggagagtacc
     1141 gccggcttaa ttttcaggat agtttcggcg agtccgtgtc cggttacgac gcgggcaagc
     1201 cgagtctgaa gttttacgag agccgcgtcg aaggcagcag tccgttacgc cgctgggaga
     1261 ttcccgaccg cggtgagcag aagggtaccg cggtcgcaca gcgccggccg gcggtggcaa
     1321 agctcggctt gacgcgcaac gtgccggcgg cggaatttaa tttcttacgc caagtcgctg
     1381 acaggcccgg caaagttgcc ttgatcggtc ccgatcgtat ctcgcagaga ttcgactggc
     1441 agaactccaa agctatttat tccgggctcg acgagtttgt cgacgacgtc gtcaaggtag
     1501 agcgtgagat cataaatgga ttggtccagg ccggttgtct ctacgtgcaa atcgatgccc
     1561 caggttagac agcttacgtg gatcggcctt cgctcgatgc catgcgcggc cgcggtgaag
     1621 atccgaaaga aaacttcagc cgctcaatca gagccgataa tgccgtgctc gcgggtttcg
     1681 acgatgtcac attcggcatc catctttgcc gcggcaacca acgcagcatg tggcaccgtg
     1741 agggcactta cgatgagatc gccgagcggc tgctcaatga attgaatcat gatcgctttc
     1801 tcttcgagta tgacacgccg cgtgcgggcg gcttcgaacc gttgagattc ttgcctaaag
     1861 ggaaggtgat cgtcttgggt ttggtgagca ccaaggtgcc gcagctcgaa accgtcgacg
     1921 agctcaaacg gcgcatcgat gccgccgcca agtacacgcc cctcgagcag atcgccatca
     1981 gtccccagtg cggcttttcc tccgatgtcg tcggtaattt aatcagcgat gacgatcaac
     2041 ggcgcaaact tgaggtggtg gtggagacgg cacgccaggt ttggggatag gcgttgctta
     2101 gctgagtcgg cgaatttaaa ttctagaact tcacggctgg agaggtgtca gagtatgggt
     2161 gacgatattg ccagaattcg ggcagcgatt cgcgatatcc ccgacttccc caggccggga
     2221 attgtcttca aagacattac gccgttattg agcaatggtc ctttgttcgg caagaccgtc
     2281 gacttgattg ctgagcgcta tcgtggccaa aaagtcgata ccgtgttggg gatcgagtca
     2341 cgcggattta tcttcggatc agtgctcgct tataagttgg gcgcgggatt tagcgtcgtg
     2401 cgcaagcccg gcaagctgcc ctacgaaacc cacagcgccg attacgaact cgaatacggc
     2461 aaggatacgt tagaaattca tgtcgatgcg gtgccgcgcg atgccagggt ggtgatcgcc
     2521 gacgacttga tcgccaccgg cggtaccgcc gccgccaccg ccgaactggt atcgaaactc
     2581 ggcgccactg tggtcgaatg tgcctttgta atcgagctca gttttcttca tggccgagaa
     2641 aaactaaagc cgcacaaggt tttttccgtg ctccagtacg actcggaata gattggcaaa
     2701 atctctgttt ttgcagcgtt tccgttcctt gacttcactt cgtattcgtc tataaattga
     2761 gatcagtgtg actgattcgc cgtgccaatg tcagtccagc gcgacatgcc gttgtcagac
     2821 catcttagga tcgataatcg atgaaacgta ttgaacagat gactctgtgg gagaggctct
     2881 atttccctga aatttttcgt ggactgatgg ttaccggcgg tcgcttctgg cgaaatttag
     2941 caattcatgc gctgcaaaag ttcggactcg ccaaagatac gagcgccgca atgacggtgc
     3001 agtatccgga agagaagtgg gtttatccag cgacctatcg cggtcgtcac cggcttaccc
     3061 tgaaacccga tgagactgtg cagtgcacag cctgttattt atgcgccacc gcgtgtccgg
     3121 ccgagtgtat ctacatcgag gccggcgagt atgccgacaa gtcggtggag aagtttccca
     3181 tacgctatga gattgatacc ttgcgctgta tctactgcgg atactgcgtc gaggcctgtc
     3241 cctgcgatgc aatccgcatg gacacgggaa ctcacccagg caatttgggt tttagccgca
     3301 aggatttcgt cgaaaccaaa gaagtgttga tgcaaagatc gaaggattta gccgagaagg
     3361 gcaaagaagg gctttacgaa gaatacgtca aaccttaccg ccacgtctaa gcacggcggc
     3421 ggtgttccag ccgaaggagg caagatcatg gcaaatacgc aatccacaaa tggcgccaat
     3481 ccgaatggcc ttacgaccca accgctgccg gcctcgcaca aggtatatgt gcatagtcat
     3541 cacc
//
LOCUS       DATLCH010000019         5299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_804113, whole genome shotgun sequence.
ACCESSION   DATLCH010000019 DATLCH010000000
VERSION     DATLCH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804113"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(903..1985)
                     /locus_tag="VMZ02_00500"
     CDS             complement(903..1985)
                     /locus_tag="VMZ02_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70430.1"
                     /translation="MGWVDADAHVVESPLTWEYLTPSEKKFRPQLFKPEDDNQKAHWV
                     IDGKIRGLFRFTFSKDDLDKKSAEIGRDVSTTMATRDLADVTARIKHMDELGIDTQIL
                     YTSIFLDQVTERADTEVALCGSYNRWMAEVWKKSNGRLRWMACLPLLSMADALDQLRF
                     AKDNGGCGVFMRPLEGARQISDPYFFPMYELAEKLNFCIGLHQANGSAAAVDMMVNPD
                     GSRDFFNQYRIFNVGAMFRILNCGIPQKFPKLRFGFIETAASWVPWVIYELRRRLDTV
                     SNKLPNDLLEKSGIFVTCQIGDDVPYLIKTCMGENTLMIGTDYGHADSSTELDALTTL
                     KEGGGISAAQHKKIVEDNPRVFYGLN"
     gene            complement(2049..2813)
                     /locus_tag="VMZ02_00505"
     CDS             complement(2049..2813)
                     /locus_tag="VMZ02_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR70431.1"
                     /translation="MGLLDGKVVVVTGGGHGIGRAYCMGIAREGGTAVVADIDDKAAD
                     GVAKAIADKGGKALATKVDVANFPSCQEMAAKTLNAYGQIDGLINNAAIFMSVPAEKG
                     TWQDITEEAWDRIMAVNVKGLWLCSRAVVPAMQKRKQGSVVNISSNMAFNGGLTMMHY
                     VTSKAAVVGFSRVLARELGSDNIRVNTLAPGSTMSEDKATDEAVKNYERSASTRILKR
                     IEQPEDLVGTALYLLSDLSTFVTGQTILVNGGAVLH"
     gene            complement(2925..3980)
                     /locus_tag="VMZ02_00510"
     CDS             complement(2925..3980)
                     /locus_tag="VMZ02_00510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR70432.1"
                     /translation="MKKLFSLFVLLFLLGYAGLAGQSWAAAADEKAVADFYHGKTVRI
                     IVGFSAGGGYDAYSRLIGRHIQKHIPGNPNVIVENMAGAGSILLANHMYNVAPKDGTV
                     IGNFSGQIILEQLFGNPAVQYDLGKFRYLGVPIGETYLLITTRKSGVTKLDELLGAKA
                     KQITVGGIPGSTVEQGPLLMRDLLGANIKVVSGYKGTSDVRLAMESGEIDGIMNSWES
                     SKVTSLDRIKNGDWQLLAQLGDNSIADLPVRNVPTIPQIAKTEEQRQLLRFGAAAPNQ
                     FGKVYVVSPGTPPERGAALELALTRTFADKELLAEAERGKLDIDPVSAQHVQKLINDF
                     FGLSPELKAKLKKILRP"
     gene            4182..4979
                     /locus_tag="VMZ02_00515"
     CDS             4182..4979
                     /locus_tag="VMZ02_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR70433.1"
                     /translation="MSNPQRKFTEVQGIKLHYLEWGERGKPDLLLVHGWTNFAGSWTA
                     VAEHFQDRYRIIAPDLRGHGESDKPQTGYRLRDFAEDIRQLIQNLGLNKPAYVGHSWG
                     GNIGTILAADSPQSISRAFLEDPVYWRMIHAFMTALPNALARRNKPEAELRAEAKQKN
                     LSKADEDMEVYRNHHFAAHALTRLLTDNRDWSFGFEDYFKRIAVPTTILVADHKAGGA
                     IMREEMGYLQSIAPRPVKLEFWENTGHGMKAAKPAEYNKALDEWLKS"
     gene            complement(5062..>5299)
                     /locus_tag="VMZ02_00520"
     CDS             complement(5062..>5299)
                     /locus_tag="VMZ02_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR70434.1"
                     /translation="ARHNFEGTHRLADVQAPTLVLIGEADTVGSNHIAQARVLMERIR
                     GAEMKVLKGQSHGFFWQAPEETNSVILEWVKSHS"
BASE COUNT         1144 a         1578 c         1387 g         1190 t
ORIGIN      
        1 gccccgcgcg acaaaaaagc cgccgccgcc gatccgaaaa gtttcgtcga tatgagtgta
       61 gtgcaggaac tggaagcgtc gggttttatc aagcagctgt acaaaaagta acccacgcac
      121 cgcgtggatg gttagttgac aatctaatgc cagaaatctc tcggtttttt ggaataatca
      181 tccgcatgtt catggaggcg ggcggaccac accatacgcc gcactttcac gcctattacc
      241 aagatcaagt atccatttat gatattcagc gaatcgagtt tgacaggttc gctttccaga
      301 cgccaagaac gtttggtttt ggcgtgggca gaactacatc aaactgaatt gctaagagtt
      361 tgggaggcat tgcaacaggg ccgtgtgcca aataagattg agcggttgaa ataacgagct
      421 ttgatatgtc acatgaaata catcgtgtag cttcgttcga aaagttggca ccatttactt
      481 tgcgcgtagc ttttgccgac ggcacggctc aaaccatcga cttccttcct attctgaagg
      541 gcgaattgta cggtccgctg caggatccag cgcttttcga ccagggctgc atagacccag
      601 aagtgcacac attaatttgg ccgaacggcg ctgacttcga tccagcgatc cttcataact
      661 ggccagaatc ggcccccgcc ttaaaaagta tggctgaaaa gtgggagtct acgaagtctg
      721 ggctagattc cacacgcacc tcgccgcttt gattggcgcg ccgctgccca cccatagcaa
      781 aaaagggcga ccagccggtc gccctcacaa ctaacttcta acttaccctt tacgtgtttt
      841 gcgttctaat ctccgaatct aactttgagc tccttgtgct ctttgattaa atcatccggc
      901 tcctaattca acccgtaaaa cacccgcgga ttgtcctcga cgatcttctt gtgctgcgcg
      961 gcgctgatgc cgccgccttc tttgagtgtg gtcaacgcat ctagttcggt cgatgaatcg
     1021 gcgtggccgt agtcggtgcc gatcatcagc gtgttctcgc ccatgcaggt cttgatcagg
     1081 tacggcacgt cgtcgccgat ctggcaggtg acgaagatgc cggacttctc cagcagatcg
     1141 ttgggcagtt tattgctgac cgtgtccaag cggcggcgca gctcgtagat cacccaaggc
     1201 acccaggatg ccgcggtttc gataaaacca aaacgcagct tgggaaactt ctgcgggatg
     1261 ccgcagttca aaattctaaa catcgcgccg acattgaata tccgatactg gttaaaaaaa
     1321 tcccggctgc cgtccggatt gaccatcatg tcgactgccg cggcgctgcc gttggcctgg
     1381 tgtaggccga tgcagaagtt cagcttctcg gccagctcgt acatcgggaa gaagtacgga
     1441 tcgctgatct gccgcgcgcc ttcgagcggg cgcatgaaca cgccgcagcc gccgttgtcc
     1501 ttggcgaacc tcaactgatc gagcgcgtcg gccatgctga gcagcggcaa gcaagccatc
     1561 cagcgcaacc gtccgttgct ttttttccaa acctcggcca tccagcgatt gtacgagcca
     1621 cacagcgcga cttctgtatc ggcacgctcg gtcacctggt cgaggaatat gctcgtgtaa
     1681 agaatttgcg tgtcgatgcc aagctcgtcc atgtgcttga tacgcgccgt aacgtcggcc
     1741 agatcgcgcg tcgccatggt ggtgctcacg tcgcggccga tctcggcaga cttcttgtcg
     1801 agatcgtctt tgctgaacgt gaagcgaaat aggccgcgaa tcttgccgtc gatcacccag
     1861 tgcgctttct ggttgtcgtc ttccggcttg aacagctggg gtcgaaactt tttttccgaa
     1921 ggcgtgagat actcccaggt cagcggactc tcgacgacat gcgcatcagc atctacccat
     1981 cccatggctt cctccaactc atttttaatg tttcgcccct actattgcct ggtgcctatt
     2041 gccttcttct agtggagcac cgcaccgcca ttgacgagaa tcgtctgtcc cgtgacaaac
     2101 gtgctcagat ccgatagcaa atatagcgcc gttccgacca aatcctccgg ctgctcgatg
     2161 cgcttgagaa tgcgcgtcga agccgaacgt tcatagttct tgaccgcttc gtcggtcgcc
     2221 ttgtcttcgc tcatagtcga gcccggcgcc agcgtattca cgcgaatatt atccgacccc
     2281 agctcgcggg caagcacacg gctgaagcct accacggccg ccttcgacgt cacatagtgc
     2341 atcatggtca acccgccgtt gaaggccatg ttcgacgaga tgttgacgac gctgccctgt
     2401 ttgcgctttt gcattgccgg caccaccgcc cggctgcaca gccacaagcc tttgacgttg
     2461 accgccatga tgcggtccca cgcttcttcg gtgatgtctt gccaggtgcc tttttccgcc
     2521 ggcacgctca tgaagatcgc cgcattgttg atcagcccgt cgatctggcc gtaggcgtta
     2581 agtgttttag cggccatctc ctggcagctg ggaaaattcg ccacgtcgac tttcgtcgcc
     2641 agcgctttcc cgcccttgtc ggcgatcgct ttggcgactc cgtcggcagc tttgtcgtcg
     2701 atgtcggcga ccaccgccgt gccgccttcg cgcgcaatgc ccatacaata agcgcgcccg
     2761 ataccatgtc cgccgccggt cacgaccacc actttgccgt ccaataaacc catctgatgc
     2821 tctcctctat cggtattatt tatcgcaatt acccgccatc gttcaatcaa acggacccgc
     2881 gggcgacctt gaactttgaa cccctgaacc ttgaaccggc cgaattacgg ccgaagaatc
     2941 tttttcagct tcgctttgag ttctggcgag agcccgaaga agtcgttgat cagcttctgc
     3001 acatgttgcg ccgacaccgg atcgatgtcg agcttgcctc tctcggcttc ggctaacaat
     3061 tctttgtcgg cgaatgtccg cgtcagggct aattcaagcg cggcgccgcg ctccggaggt
     3121 gttccggggg ataccacgta gactttgccg aactgattgg gcgccgccgc gccaaaacgc
     3181 agcaactgac gctgctcttc ggtcttggca atctgcggga tggttggaac atttctcacg
     3241 ggtaaatccg cgatcgagtt atcgcccaat tgcgcgagca attgccagtc gccgttcttg
     3301 atgcgatcca aggaagtaac tttcgaagat tcccaactgt tcatgatgcc gtcgatctca
     3361 ccgctttcca tcgccaagcg cacgtccgaa gtgcctttgt aaccgctcac tactttgata
     3421 ttggcgccca acagatcgcg catcagcaac ggtccttgtt cgaccgtcga gcccggaata
     3481 ccgccgacgg tgatttgctt tgccttggcg ccgagcaatt cgtcgagctt ggtcacaccc
     3541 gacttgcgcg tcgtgatcag caaataagtc tcgccgatgg gcacgccgag gtagcgaaac
     3601 ttgccgagat cgtactgcac cgcggggttg ccaaacagct gctcgagaat aatctggccg
     3661 ctaaagttgc cgatcaccgt gccatctttg ggcgccacgt tgtacatatg gttggccagt
     3721 agaatgctgc cagctccggc catgttttcg acgatgacat tgggattgcc gggaatatgt
     3781 ttctgaatat gccggccgat caagcgggaa taagcatcgt agcctccgcc cgcggaaaac
     3841 ccgacgataa ttctcaccgt cttgccgtgg tagaaatccg ccaccgcctt ttcgtccgcc
     3901 gccgcggccc aactttggcc agccaagccc gcatatccga gcaagaacag gagcacaaac
     3961 aacgagaata actttttcac ggttacctcc gtggaagaca ttctttcgta gccttggcta
     4021 tcaccctgct tcagcgactg tcaacagtga gatggcggca agagtctggc ggcgacggtg
     4081 gaacatgaaa cgtttggccc ctagactctg aaaaaaagtt gggataatca ctagaggccg
     4141 cgcagccatg ggaaaaatct agagcgcaaa tgaggacgtt aatgagcaac ccacaacgca
     4201 aatttaccga agtgcaagga atcaagctgc actacttgga atggggcgag cgcggcaaac
     4261 ccgatctgct gctggtccac ggctggacga acttcgccgg gagttggact gcagttgccg
     4321 agcacttcca agatcgctat cgcatcatcg ctcccgactt gcgcggccat ggcgagtcgg
     4381 acaagccgca gacgggttat cgcttgcgcg acttcgccga ggacattcgc cagctgattc
     4441 agaatctcgg attgaacaag cctgcatacg tcggccactc ttggggcggc aatatcggca
     4501 cgatcctggc cgccgactct ccgcaatcga tctcgcgcgc gttcctggaa gatccggtct
     4561 actggcgcat gatccacgcc tttatgaccg cactgcccaa tgcgctggcg agacgcaata
     4621 aacccgaggc tgagctccgc gctgaagcga agcaaaaaaa cttgtcaaag gctgacgaag
     4681 acatggaggt ctaccgcaat catcacttcg ccgcccatgc gctaacccgc ctgctcacgg
     4741 ataatcgcga ttggtcgttc ggcttcgaag attatttcaa acgcatcgcc gtgccgacaa
     4801 caatcctggt ggccgaccac aaagccggcg gcgccatcat gcgcgaggaa atgggctacc
     4861 tacaaagcat tgcgccacgg ccggtgaaat tagaattctg ggaaaacacg ggccacggca
     4921 tgaaggccgc caagccggcg gagtacaaca aagcgctcga tgaatggcta aaaagctaac
     4981 cggtggccgc gccaattcga tgtaggggcg cagcatgctg tgcccgatca tttcggtcat
     5041 accccgacca cggaatccct ttcagctatg gctcttcacc cactccagaa taactgaatt
     5101 ggtttcctcc ggcgcctgcc aaaaaaatcc atgcgactga cctttgagta ctttcatctc
     5161 ggcgccgcgg atgcgctcca tcagcactct ggcctgggcg atgtgattgc tcccgacggt
     5221 gtccgcctcg ccgatcaaca ccaacgtcgg cgcttgaacg tcggccaacc ggtgcgtgcc
     5281 ttcgaagtta tggcgcgcg
//
LOCUS       DATLCH010000020         4889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5281923, whole genome shotgun sequence.
ACCESSION   DATLCH010000020 DATLCH010000000
VERSION     DATLCH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5281923"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VMZ02_00525"
     CDS             <1..470
                     /locus_tag="VMZ02_00525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR70435.1"
                     /translation="HLHYSSVALGIYIALLHAMGTISQPLLGLLSDRFGRKTVLMPSC
                     LLLGVLFALLAIVSPGIPLGLVIVAIGLFFYTLFNIFNAAVMDVASSNAQAATYGLTS
                     LITQLVVIPAPMITGYLIGGLGIRFAFLLAGAFLVVAGLVLAPLQLYRGTQNS"
     gene            670..1554
                     /locus_tag="VMZ02_00530"
     CDS             670..1554
                     /locus_tag="VMZ02_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HUR70436.1"
                     /translation="MVRSKTAIPSDDLIVHLIDGTYELFRHFYGLRRFNKGKDAPFGA
                     VIGVLNTVLQMIETGATHVGVATDHVIESFRNALWSGYKTGDGIEPALLAQFHPLEEA
                     LTAMGVAVWPMIELEADDALASAAQIACSNEQVQKVCIWTPDKDLAQCVRGDRVVQVD
                     RRSHKIRDAAGIREKFGVEPLLIPDFLALVGDAADGYPGIAGIGAVSAARLLNRHGAI
                     ENFPANVLGDSRDLALLFKRLATLRIDAALFHDVADLRWRGPTDAFGECAERLSESRL
                     VQRALAAHKSLTDGSSRT"
     gene            1722..2357
                     /locus_tag="VMZ02_00535"
     CDS             1722..2357
                     /locus_tag="VMZ02_00535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70437.1"
                     /translation="MNHCRRHALLCAVLVGLLFSAGKVLQAQTLRVPYVSLSPTAGPL
                     WIAYEAGLFKRNNVNTELLYIPGGSTIIQAIISGDVKIANMAPPSAIGAWAKGFDLVL
                     IASGVDQLLETVVTGPAIKSPADLKNKKIGVSRYGSLTDMALREALRQFKLNPDKDVT
                     ILQTGGEATRFAALTTGAIDGAMLSGDKLVQAEKLGYHVTSISASCRFIIQ"
     gene            2354..2728
                     /locus_tag="VMZ02_00540"
     CDS             2354..2728
                     /locus_tag="VMZ02_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70438.1"
                     /translation="MNGVIASKKFLAGNRDVAKGFLRSWVEAIKVFKTDKEFSLKVLA
                     KYLKIQDREILEKSYEIYRPVYKKIPYGDRRAVSFALDQMGKEIPDAAKLNADNFIDN
                     SILAELEKSGFIDQIYNETSIK"
     gene            2739..3539
                     /locus_tag="VMZ02_00545"
     CDS             2739..3539
                     /locus_tag="VMZ02_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR70439.1"
                     /translation="MLAKIKHLAIISDNYVLLSRFYEALFGMKTSKSPRPESAVAIGD
                     GYIGMNIIPQKLGRQAGLEHFGLEVEDVETVADRLREKYPAIQLVKRPTNRPFAGIST
                     HDPDGYVFDLSQQAMSNRAEVYVEGEWKQERHISHFVLRSLNPANLAKFYREVYELQE
                     LEKPADDPNHYLSDGRVTMVVSPWAISDYDGAGIEPRHMDHLGFKVEDVEGFKKDLKA
                     LVNRNPALAPKPGGTEGEIRLRLLAKCKHGQFQLADPDGVPIDVSAGR"
     gene            complement(3621..4730)
                     /locus_tag="VMZ02_00550"
     CDS             complement(3621..4730)
                     /locus_tag="VMZ02_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70440.1"
                     /translation="MSSSFLQSMAVLEGARAALKVGAVSKGERVGFICDLRVESDVIQ
                     AFFTAAAEIGATPFLCMVERGRGYGPPDEFIETIKTANVLYFSWEMANSLVIKALRQE
                     RGIRCVGFPHCRTAALLSDDAVRFPLDVLSAIYPKTWDVFRCGGDVEVHITDTKGTDF
                     RVTLTKENIEEKFAQDPRYSGQIIANKPGFVSHLPVGHGPNVLSPSEPHNATSEGTIR
                     LDALTLAPGVYRGWSGDSAYQEPVTCRMKQGRIVAIEGGKEADMFRRVIEEDLDNVCQ
                     LREIGVGHNPKVPTFRGERGYHGANHAGAVHWGFGGGHQKKRVHVDAIIFQTTVKANG
                     TVIIDRGRLKALDDPEVRRLASQYGDPDRVLSEAL"
BASE COUNT         1095 a         1358 c         1350 g         1086 t
ORIGIN      
        1 agcatttgca ttactcgagc gtcgcactgg gaatatatat agctctgctg catgccatgg
       61 ggacgatttc gcaaccgcta ttgggcttgc tttccgaccg cttcggccgc aagacagtgc
      121 tcatgccgtc ttgccttttg ctcggagtgc tttttgccct tttggcaatc gtgtctcccg
      181 gcattccgct cggcctggtg attgtcgcga tcggtctctt tttctacact ctcttcaata
      241 tcttcaatgc cgccgttatg gacgtcgcta gctccaatgc ccaggcggcg acctacggac
      301 tgacttcgct gatcacacag ctcgtggtca ttccggcccc gatgatcacg ggttacttga
      361 tcggcggatt ggggatcagg ttcgcttttc tattggcagg agctttcctt gtcgtcgccg
      421 gcctggtgct cgccccctta cagttgtatc gcggaacaca aaattcataa gagggctcaa
      481 gatcacttgt tgacattgga ccgaggtgtt gatgtcttca gcggcaaaaa cctgatcgac
      541 cagcgcatcg gccgaaagct tgaaaaatac cgtgtcttca aatatcgcgg gacgtcgaag
      601 gatagctggc ggcgactatg atgaaaaaga ctgcgttgcg gtgattctaa tcgtttagcc
      661 cggacggaaa tggtcagaag caaaactgcg attccttcgg acgacttgat cgttcatctg
      721 atcgatggga cgtacgagtt attccggcat ttttacggtc ttcgtcgttt caacaaaggc
      781 aaggatgcgc cctttggcgc agtgatcgga gtcctcaata cagttttgca gatgatcgag
      841 actggagcga ctcacgtcgg tgtggcaaca gatcacgtca ttgagtcttt ccgcaacgct
      901 ctgtggtccg gctacaaaac cggcgacggc atcgagcctg cactgctcgc ccaattccat
      961 ccgctcgaag aagcactcac cgccatgggt gtggctgtat ggccgatgat tgagctcgag
     1021 gccgacgacg cgctcgcctc tgccgcacag atcgcctgct ccaacgagca agtgcaaaaa
     1081 gtctgcatct ggacacccga caaggacttg gcgcagtgcg tccgcggtga ccgcgtagtg
     1141 caggtcgatc gccgaagtca caagatccgc gatgccgcag ggattcgcga gaagttcgga
     1201 gtggagccgc tcctgattcc cgactttctc gctctcgtcg gcgacgcggc cgacggctat
     1261 ccgggtattg ccggcatcgg cgcagtcagc gcggcacggc tgctgaacag acacggggct
     1321 atcgagaact tcccagcaaa cgtcctaggt gacagccgcg accttgcact attattcaaa
     1381 cgtcttgcga ccctgcggat cgacgcggct cttttccacg atgtggccga cttgcgctgg
     1441 cgcggcccta ccgatgcatt tggcgagtgc gcggagcgct tgagcgagtc gcgcttggtg
     1501 cagcgcgctc tggcggcaca taagtcactc actgacggat ctagtcgcac gtgatgagcc
     1561 acctgttcaa acgcgctgaa gacaaaaggg ttggccacag tttttcttac gcgcgctgac
     1621 acaagtcatc aaaacggttc ataacactgc ggcgttgcag gtttttgacg cacttggtac
     1681 cgtcgcggct ggagcagcaa agatcgccgg aaaggtcact gatgaatcac tgtcgccgac
     1741 atgcactgct atgtgctgtt ttagttggac ttctcttttc cgcgggaaaa gttctccagg
     1801 cccagacgtt gagagttcct tacgtgtcgc tgtctccaac cgccggaccg ttgtggatcg
     1861 cctatgaagc gggattgttc aaacgcaaca acgtaaatac cgagctgcta tacattcccg
     1921 gcggctcgac gattattcag gcgatcatct ccggcgacgt caagatcgcc aacatggctc
     1981 ctccctcggc gatcggagct tgggctaaag gcttcgactt ggttttaatc gccagcggag
     2041 tcgatcagct cttggaaacg gtcgtcaccg gacctgccat caaaagtccc gctgatctca
     2101 agaataaaaa gatcggcgtc agccgatacg gctcgctcac cgacatggcg ctgcgcgagg
     2161 cattgcgcca gttcaagctc aatccggaca aagacgtgac gatattgcag accggcggcg
     2221 aagcgacccg atttgctgct ttaacgactg gcgccatcga tggcgcgatg ctttcggggg
     2281 ataaattggt tcaggccgaa aagttgggct atcacgtaac atcgatctca gcaagctgcc
     2341 gatttattat ccagtgaacg gcgttatcgc ttcgaagaag tttctcgccg gcaatcgcga
     2401 tgtggccaaa ggctttttaa gaagttgggt cgaagccatc aaagttttta aaaccgacaa
     2461 ggagttttcg ctcaaggtgc tggccaagta tctcaagatc caggatcgcg agatcttgga
     2521 gaagagctat gaaatttacc gcccggttta caagaagatt ccctatggcg accgacgggc
     2581 ggtgagtttc gcgctggatc aaatgggcaa agaaattccc gacgcagcca agctcaacgc
     2641 cgataatttt atcgacaaca gcatcttggc cgagctcgaa aagagcggtt tcatcgatca
     2701 gatctataac gaaacttcga tcaagtaagg agaccgccat gttagcaaag atcaagcacc
     2761 tcgccattat cagcgacaac tatgttctgc tgagccggtt ttacgaagcg ctgttcggca
     2821 tgaagacatc gaagtcgccg cggccggaga gcgcggtggc gatcggcgac ggttacatcg
     2881 gcatgaacat cattccgcag aaattgggac ggcaggccgg attggagcat tttggcttgg
     2941 aagtcgagga cgtcgaaacc gtcgctgacc ggctgcgtga aaagtatccc gcgattcaat
     3001 tggtcaagcg ccctaccaac cggcccttcg ccggcatcag cacgcatgat cccgacggct
     3061 acgtgttcga tttatcgcag caagcgatga gcaaccgggc ggaagtctac gttgaaggcg
     3121 aatggaaaca ggagcgtcat atcagtcatt tcgttttgcg ctctttgaac ccggcgaatc
     3181 ttgccaagtt ttatcgcgaa gtttatgaac tgcaggaact cgaaaaaccg gctgacgatc
     3241 cgaaccatta tttaagcgac gggcgagtca cgatggtggt ttcgccgtgg gcgatcagcg
     3301 actacgacgg cgcgggaatc gaaccgcgtc acatggacca tctgggattt aaagtggagg
     3361 acgtcgaggg ctttaaaaaa gatctcaagg ccttggtcaa cagaaatccc gccctcgcac
     3421 ccaaacccgg cggcacggaa ggcgagatca gactcaggct gttggcgaaa tgcaagcatg
     3481 gccaatttca gctcgccgat cccgacggcg tgcccatcga cgtttccgcc ggtcgctagt
     3541 taagatcagc tattgacgaa gcgcgccgcc gcagcctcca aattcgccaa ccgcgccaat
     3601 gagcaaacac ccgtgcgcat ttacagcgct tccgacaata cgcgatcggg atcgccgtat
     3661 tgacttgcca atcggcggac ctccggatca tcgagagctt tgaggcggcc gcgatcgatg
     3721 atcaccgtac cgttggcctt aacggtagtt tgaaaaataa tcgcatcgac gtgaacgcgt
     3781 tttttctgat ggccgccgcc aaaaccccaa tggaccgcgc cggcatgatt ggctccgtgg
     3841 tatccccgct cgccgcgaaa agtcggaact ttcgggttgt ggccgacgcc gatctcccga
     3901 agctggcaaa cattatctaa atcctcttca atgacgcggc gaaacatatc ggcctcttta
     3961 ccgccttcga tggcgacaat gcggccttgt ttcattcggc aggtcaccgg ttcttgatag
     4021 gccgaatcac cggaccagcc gcggtagaca ccgggcgcaa gcgtcagagc atcgaggcga
     4081 atggtccctt cactggttgc gttgtgcggc tcggatgggc tcagaacatt gggaccgtgg
     4141 ccgaccggca gatggcttac gaagccaggc ttgttggcga tgatctgacc ggagtagcgc
     4201 gggtcctggg caaatttttc ctcgatattt tccttggtga gggttacgcg aaagtcggtt
     4261 cctttggtat cggtgatatg gacctcgacg tccccgccgc agcgaaagac atcccaggtc
     4321 ttggggtaga tggcagagag cacgtccagc ggaaagcgca ccgcgtcgtc cgacagcagc
     4381 gccgccgtgc ggcagtgcgg aaaaccgaca cagcggatgc cgcgctcttg gcgcagcgct
     4441 ttgatcacca acgagttcgc catctcccag gaaaaataaa gcacgttggc ggttttaatg
     4501 gtttcgataa actcgtccgg cggaccgtaa cctctgccgc gctcgaccat acacaagaac
     4561 ggagtcgcgc cgatctcggc ggcagcagtg aagaaagcct gaatgacgtc ggattcgacg
     4621 cgcaaatcgc aaataaaacc gacgcgctct cccttgctga ccgcgccgac tttcaaagcg
     4681 gcgcgcgcgc cctccaaaac cgccatcgat tgtaaaaaac tggacgacat tttcattctc
     4741 cctctagtcc gcgaacctat ttggctattt atttttaatc attggtaaac tgctagtttc
     4801 agtgtgtcaa ccagacttca cagacggctt gacttgctta cgcggcggct ttattaaaat
     4861 tgccatctct gatcagcgcc ttgatgact
//
LOCUS       DATLCH010000021         5625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2300567, whole genome shotgun sequence.
ACCESSION   DATLCH010000021 DATLCH010000000
VERSION     DATLCH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300567"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VMZ02_00555"
     CDS             complement(<1..334)
                     /locus_tag="VMZ02_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR70441.1"
                     /translation="MALTPQENADLTRVGPGTPCGTMMRRYWHPVGFTAELQGKPVRR
                     KLLGEDLVLFRDEKGQLGLIGARCPHRGTSLHLGYIEDGGLRCCYHGWLFDVNGRCLE
                     QPTEPAEAN"
     gene            complement(674..1735)
                     /locus_tag="VMZ02_00560"
     CDS             complement(674..1735)
                     /locus_tag="VMZ02_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70442.1"
                     /translation="MISIRNLCKIYKSGRDAVHAVDGVSVEVGAQEFFVLLGLSGSGK
                     TTLLRCVAGLERPDGGEIAISNVLVSSAERNFFVAPEGRGIGMVFQSYAVWPHMTVFD
                     NVAFPLGNGPYKLSRADIKKRVMNALELVQLAGLAHRPAPFLSGGQQQRVALARALAV
                     EPKVLLMDEPLSNLDARLREEVRDEIKNLARKFSIAVLYVTHDQVEAMALADRVAVMS
                     KGKVLQCAPPRELYDRPASREVCEFLGSTNVIDGVIGSRGEVDTDLGKLNCAVSNGLH
                     DVSVAIRPEELLLSATPTGLENEFAAEVLASTFLGELTVCDVKINGKALRFKTTQSTA
                     PAQQVYIRLPADKLKLFAR"
     gene            complement(1847..2620)
                     /locus_tag="VMZ02_00565"
     CDS             complement(1847..2620)
                     /locus_tag="VMZ02_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HUR70443.1"
                     /translation="MFILKQFLKALLLPPTPWLLLLLTVLIFWQRRWARKLLFLTFLL
                     ILGLHCGPANYALRYQLESRYSPLLDPRKAGPYDAIVVLTSGSMQASGLIPFPSVDEH
                     MFRRLDEAWRLYRIQPKPIIISGGHVNPFTPAMDENRIARDFLIKWGVPKHDVIGEEK
                     SRDTFENALESQKILKKRGWKRYLLVTSAMHMPRSMLVFSARVPDPIPAPGDFSIGSF
                     ELSPFDFFPNESVAPKMFATIHEYIGWANYYWRLHYSAD"
     gene            complement(2718..3362)
                     /gene="maiA"
                     /locus_tag="VMZ02_00570"
     CDS             complement(2718..3362)
                     /gene="maiA"
                     /locus_tag="VMZ02_00570"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HUR70444.1"
                     /translation="MKLYTFFRSSASYRVRIALNFKGLSCAQAPVHLRRGGGEQLMQA
                     YKTINPQALVPALEDNGRILTQSLAIIEYLEETHPNPPLLPKDPADKALVRSMAMVVA
                     CEIHPIQNLRVLQYVKANYNQTDEQINKWAQHWIDLGLSALQEMIIAQPKRGRFCFGD
                     SATLADICLIPQLGNARRYGCDLSKYPTILEIENNCNALPAFADAAPEKQPDAE"
     gene            complement(3457..4446)
                     /locus_tag="VMZ02_00575"
     CDS             complement(3457..4446)
                     /locus_tag="VMZ02_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70445.1"
                     /translation="MKALLILVCLLLATLAVAQDAKLIAQGKKEGKVVIYGSMETDIF
                     EGIQKSFEKKTGITVEYWRAAGATVLERASTERKANKVAYDLVLNNAGPMEILLTEGA
                     LAKYDSPLAKNFPAEFVHPQLGPSYRTSIVGIIYNKSMISPDKAPKSLEDLLKPEFSG
                     KLAFPDPSRGAVAVMWPMSLYKLMGKDRADKYLRDLGATKPVIVEGVLPAAERVTSGE
                     TPIAISYLKYVVNFGQKGAPLDYVRQEKMLGHNHAIALSNKAAHPNAAKAFLDYFYSD
                     DSLKIMAKSGEFMTRKGIYPALADAEKIQVVPMDEPDAATMAEKRKEYRKIFQ"
     gene            complement(4562..5530)
                     /locus_tag="VMZ02_00580"
     CDS             complement(4562..5530)
                     /locus_tag="VMZ02_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70446.1"
                     /translation="MSGTNGKKALHVATFSGWYRFEQHGKQFTQTKRDLSYWTLTCMT
                     VDPQNPNTLYAGTEHSGLFFTTDAGAHWQRSDPQVPKMFLYSALALNGGVMVGTIPSA
                     VYRSKNGGWEELEGVRLHSAGANFPPSPELQSRTRYLAYDPENTNRLYAGIEVGGMVL
                     SDDGGRGWEPCNEGLTDMDIHEILASEQHRGMVFLACGDSCFRSADRAGHWENISPKN
                     HDYGISVAEDNNGVLYVGAARGRPNLWLREGGAMSAILRSSDKGKSWETVIDNLNGGV
                     MHLCPTPDGHGMVAGTSDGTLLFVDDSGAREVVSGLPFVTSVELAA"
BASE COUNT         1176 a         1524 c         1635 g         1290 t
ORIGIN      
        1 aatttgcttc ggcaggttcg gtcggctgct ccaaacagcg gccgttcacg tcgaatagcc
       61 aaccgtgata acagcagcgc aagccgccgt cttcgatata gcccaggtgc agtgacgtgc
      121 ctcggtgcgg gcagcgcgcg ccgatgaggc ccaattggcc tttctcgtcg cgaaacagaa
      181 ccagatcttc gccaagtagt ttgcggcgca ccggtttgcc ttggagttcg gcggtgaacc
      241 cgaccggatg ccaatagcgg cgcatcatcg tgccgcacgg cgttcctggt ccgacgcggg
      301 ttagatcggc attttcttgg ggcgtgagtg ccatttcgat gtctcctgcc taatgcgaaa
      361 ttctagagct gattgggaaa tcgtttttac cacaaagatc gcgaatttca cgaaggattc
      421 ggatgaataa tcttttccga aacttcgtgc tcttcgtgtt ctccttggaa atgcatgtgc
      481 aggtacgaat cggggcgacg aattggcatg aggctttcgt catgccggcg caagcctgca
      541 tccaggggcg ggaggggatg gataccggct ttcgcgggtt tgaaaaatca acggagcaac
      601 catgtggtgc agggagggcg gcaattttat actttgtaga ggagcgtaag ctcatggaca
      661 cttcgtggtg aattcatctc gcaaacaatt tcaacttatc agccggtaaa cgaatgtaaa
      721 cttgctgtgc cggcgccgtg ctttgggtgg tcttgaagcg taaagctttg ccgtttattt
      781 tcacgtcgca caccgtcagc tcgccgagaa aagtcgatgc caatacttca gcggcaaatt
      841 cgttctccaa gccggtgggg gtggcggaca acagcaactc ttcgggacga atggcgacgc
      901 tgacatcgtg caagccattg ctgacggcgc agttgagctt gcctaaatcg gtgtcgactt
      961 caccgcggct gccgatcaca ccgtctatga cattcgtgga accgagaaac tcgcatactt
     1021 cccgactcgc tggccgatcg taaagttcgc gcggcggcgc gcactgcaag actttgccct
     1081 tgctcatcac cgcgacgcgg tctgccagcg ccatcgcttc gacctggtcg tgggtaacgt
     1141 agagcaccgc gatgctgaat ttcctcgcta gatttttgat ctcatcgcgg acttcttcgc
     1201 gcagccgcgc gtcaaggttg ctgagcggct cgtccatcag cagcaccttc ggctcgacgg
     1261 cgagcgcgcg tgccaaagcg acgcgctgct gctgtccgcc gctcaagaat ggcgccggac
     1321 ggtgcgcgag gccggcgagc tgtaccaatt ctaacgcgtt catcacgcgc tttttgatgt
     1381 cggcacggga tagtttgtac ggtccattgc cgagtgggaa ggcgacattg tcgaacacag
     1441 tcatatgcgg ccagaccgca taggactgga aaaccatgcc gatcccgcgc ccttctggtg
     1501 ccacgaagaa gttgcgttcg gcggagctga ccaaaacatt gctgatggcg atctcgccgc
     1561 cgtcaggtcg ttccaagccg gcgacgcagc gcaaaagcgt tgttttgccc gacccgctga
     1621 gccccaacag cacgaagaat tcctgtgcgc cgacctcgac gctgacaccg tcaacggcat
     1681 gcacggcatc cctgccggat ttgtaaatct tacaaagatt tcttatcgaa atcatgattt
     1741 tcttggatcg atcggtcgtc gtggccacgc tgcgcgcgaa attcaagcgc agtttttatc
     1801 actctgccgg ggatttgcaa ctcgacctgc gcagactttg cggcgcttaa tcggcggaat
     1861 agtgcaggcg ccaatagtag ttcgcccagc cgatatactc gtggatggtg gcgaacattt
     1921 tcggcgcgac actttcattg ggaaagaaat caaaaggcga taactcaaag ctgccgatgg
     1981 aaaagtcccc aggggcggga attgggtcgg gcactcgcgc gctgaacacc agcatgctgc
     2041 gcggcatatg catggccgag gtgaccagaa gatagcgctt ccagccgcgt ttcttcagaa
     2101 tcttctggct ctctagagca ttctcgaaag tgtcgcggga tttctcttcg ccgatgacat
     2161 catgtttggg cacgccccac ttgatgagaa aatcgcgcgc gatccggttc tcgtccattg
     2221 ctggagtgaa tggattgaca tggccgccgg aaatgatgat cggcttgggc tggatgcgat
     2281 ataaccgcca ggcttcgtcg agacggcgaa acatatgctc gtcaacggaa ggaaatggga
     2341 tcaagccgct tgcttgcatg ctgccgctcg tgagtacgac gatggcatcg tatggaccgg
     2401 ctttgcgcgg atcgagcagc ggtgaatagc gcgactcgag ttgatagcgc agcgcgtaat
     2461 tggccgggcc gcagtgaagg cccaagataa gcagaaaagt taaaaacagc agcttgcgcg
     2521 cccaccgacg ttgccagaag atcaacacgg tgagcagcaa cagcagccac ggtgttggcg
     2581 gcagcaaaag cgctttaagg aactgcttga gaatgaacat ggcagaagcg attgtcagtc
     2641 gtcaacgatc actcaacagt tttggatggc gagcgatgcg aggtagtaac cgagaaccga
     2701 cgaccgatca cttccgatca ctccgcatcc ggctgttttt ccggcgcggc atcggcgaag
     2761 gcgggaagcg cattgcagtt gttttcaatc tctaaaatcg ttggatactt cgacaggtcg
     2821 cagccgtagc gccgcgcgtt gccgagctgc gggatgaggc aaatatccgc cagcgtggcg
     2881 ctgtcgccga agcaaaacct gccgcgcttc ggttgggcga tgatcatctc ttgaagcgcg
     2941 gacaggccga gatcgatcca gtgctgcgcc catttgttga tctgttcgtc ggtctgattg
     3001 tagttcgcct tgacgtattg gagcacgcgg agattctgga tcgggtgtat ctcgcaagcc
     3061 acgaccatgg ccatgctgcg caccagcgct ttatcggccg ggtctttggg cagcagcggt
     3121 ggattgggat gagtttcttc gagatattca ataatcgcca acgactgcgt gagaatcctg
     3181 ccgttatctt cgagcgccgg caccaaggct tgcggattga tcgttttgta ggcctgcatc
     3241 agttgctctc cgccgccgcg gcgcaggtga accggcgctt gtgcgcagct taatcccttg
     3301 aaattgagcg cgatgcgcac tcgatacgaa gcggagctgc ggaaaaaggt atagagcttc
     3361 attggcaatc ctcgaagtgg agtggtggag tattcaaaca ttgagtgatg gaagtccgga
     3421 cccaatactc catcactcta ctttacatag aagagtttat tgaaagatct ttctgtattc
     3481 tttacgtttc tcggccatgg tggcggcatc gggttcgtcc atcggcacga cttggatttt
     3541 ttcggcatcc gccaacgccg ggtaaatgcc tttgcgggtc atgaattcac cggacttagc
     3601 catgatcttc agactgtcat cgctgtaaaa ataatctaga aacgctttcg ccgcgttggg
     3661 atgcgccgct ttgttactga gcgcgatcgc atgattgtgg ccgagcattt tttcctggcg
     3721 cacgtaatcc aaaggcgcgc ccttttgacc aaaattgacg acatatttga gatagctgat
     3781 ggcgattggc gtttcaccgc tggtgactct ttcagcggcg ggcaatactc cctccacgat
     3841 gaccggtttc gtcgcgccaa ggtcgcgcag gtatttgtcg gcgcggtctt tgcccatgag
     3901 tttgtatagg ctcatgggcc acatgacggc gaccgcgccg cggctcgggt cggggaacgc
     3961 cagtttgccg ctgaattcgg gttttaaaag atcttccagc gacttcggcg ccttgtcggg
     4021 actgatcatg ctcttgttat aaatgatgcc gacgatggac gttcgatagc ttggccccaa
     4081 ctgaggatga acgaactcgg cggggaagtt tttcgccaat ggggaatcgt atttggccaa
     4141 cgctccctcc gtcagcaaga tttccatcgg cccggcgtta ttcaaaacca gatcgtaggc
     4201 aactttgttg gctttgcgct cggtggatgc acgttcgagc accgtggcgc cggccgcgcg
     4261 ccagtattcg acggtgatgc cggtcttttt ctcgaaagac ttttgaatgc cctcgaaaat
     4321 gtcggtctcc atcgagccgt agatgaccac cttgccttcc tttttcccct gagcgatcag
     4381 tttggcgtcc tgggcgacag ccagggtggc gagcaataga caaacaagta ttagtagtgc
     4441 cttcatgtgg ctctcacagg ttgatcagaa aaggatgaag gcggaaggat gagcgatgaa
     4501 gttcgatgtg aaaaaccacc actctgcaac ccttcatcct tctgccttca tcctcagttt
     4561 cttacgccgc caattccact gaggtgacaa atggcaggcc gctgacgact tcgcgcgcgc
     4621 cactgtcgtc gacgaacagg agcgtgccgt ccgatgttcc tgccaccatg ccgtggccgt
     4681 ccggtgtcgg gcacaggtgc atgacgccgc cgttcaaatt gtctatcacg gtttcccagc
     4741 tcttcccttt gtcgctgctg cgtagaatgg ccgacatcgc gccgccttcg cgcagccaca
     4801 ggttaggccg gccgcgcgcg gcaccgacgt agaggacacc gttattatct tctgccaccg
     4861 agatgccgta gtcatgattt ttcggcgaga tgttttccca gtggccggcg cggtcggcac
     4921 tgcggaagca ggagtcgccg caggcgagaa acaccatgcc cctgtgttgc tcggacgcga
     4981 ggatttcatg gatatccatg tcggtgagcc cttcgttgca gggctcccaa ccgcggccgc
     5041 catcgtcgct caagaccatg ccgccgacct cgatgccggc gtaaaggcga tttgtatttt
     5101 ccgggtcata ggcgagatag cgtgtacgcg attgcagctc tgggctcggc ggaaaattcg
     5161 cgccggcgct gtgcaaccgc acgccttcca actcttccca gccgccgttc ttgctgcggt
     5221 acacggcgga ggggatcgtg ccaaccatca cgccgccgtt caatgccaag gccgagtaga
     5281 gaaacatctt tggcacctgc ggatcggagc gctgccagtg ggcgccggcg tctgtggtga
     5341 aaaaaagacc cgagtgctcg gtgccggcat agagtgtatt gggattttgc ggatcgacag
     5401 tcatgcaggt cagtgtccaa taagaaagat cgcgcttggt ttgggtaaac tgtttgccgt
     5461 gctgctcgaa acggtaccag ccggaaaatg ttgcgacgtg caacgccttt ttgccgttcg
     5521 tgccgctcat gataagtctc cttcgaaaaa tccgagtaaa ttttgcctta gtattaaccc
     5581 gataaagagt ggattcgcaa atggtgagag ttgacgggcg agcgt
//
LOCUS       DATLCH010000022         6521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3018581, whole genome shotgun sequence.
ACCESSION   DATLCH010000022 DATLCH010000000
VERSION     DATLCH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018581"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VMZ02_00585"
     CDS             complement(<1..576)
                     /locus_tag="VMZ02_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUR70447.1"
                     /translation="MTSIPAEFYALLNAFLFAVHNIFTKKALRYSNPATGVISSLLIN
                     IVFLWTMSFLFVPMAHVTLASLMIFVGVGIFQPGLTRLLTYKGIDSLGVAITDPIRAS
                     TPLFSAAMAIVFLGEEITLPIVIATFMIISGITLLSWRHGAMKLAGSAVFLWYPIAAS
                     ALAGASQVVRKFGMAAVPHPFLAAAVTASSSL"
     gene            complement(679..2532)
                     /locus_tag="VMZ02_00590"
     CDS             complement(679..2532)
                     /locus_tag="VMZ02_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HUR70448.1"
                     /translation="MSEATATRAQYEYIVVGSGAGGGTVAARLAEAGRSVLLLEAGGD
                     PCELADSRLPEDYQVPAFHAFAAENPAMRWDFFVRHYANEQRQRRDSKYCPDHAGVLY
                     PRAGTLGGCTAHNAMIFVYPNNQDWDDIALLTGDSTWRASNMRRYFELLEDCRHRPLK
                     RWIARLTGFNPARHGFGGWLSTELAMPMETLGNRRLRRVLEELARDASLEIGDRLKRL
                     HGLLKTRADPNDWRQITASASGLRLVPLTTRDHLRMGSRERVLSIAKKFPHRLHVECH
                     ALATRVLFDGEQRAIGVEYLKGERLYRAHANVSDSAGEIRRMRASREVILCGGAFNTP
                     QLLMLSGIGPREQLIRHGIQSRVDLDGVGKNLQDRYEISVVNRMKDNWPMLAGAQFAK
                     NDSQYHDWLARKDGVYTTNGNVLALVKQSKQDRVPDLFCAGLVGNFRGYFPGYAKLIA
                     ERQNYLSWIILKARTRNRAGTVALRSADPRQPPQIHFNYFDEGSADEGKDLDAVVEGI
                     QFVRSMTRGLKKQGVIAAEELPGEQYQTDDELREFVRDNAWGHHASCSCAIGARDHGG
                     VLSSDFRVHGTNGLRVVDASVFPRIPGFFIASAIYMIGEKAAEVILTSARN"
     gene            complement(2560..4953)
                     /locus_tag="VMZ02_00595"
     CDS             complement(2560..4953)
                     /locus_tag="VMZ02_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70449.1"
                     /translation="MDLRFRSSVVMLVAWLALGGCGKKKPDFGTVKDEALTVGRAAQS
                     FPAADEDYFQQMDGGIELSVAEVKGRNNWLVWSAGNDRFWDHLVNKSFGVVDLLKIIS
                     SHPKLPHLSRDKRWEYLGVTNEPCFEKAKAPNRDRFGLWLDTRSKDCPADPFENEQKY
                     PGVRIGARGKNLPVGSYYGYATGILGLRLFPNPDFDEAAAKKWDAERFYTDPKYYNDK
                     NLVRPYRVGMACGFCHVGPSPVKPPDDPENPQWENINSNPGSQYFWVDRVLFWERDSK
                     NFIYQLIHTSLPGTLDTSFISSDYINNPRTMNAIYNVGARLKIAERWGEEKLAGGGLD
                     NKQFQQYPRTAALAQYYKEPDKVWTAHVLKDGADSVGILGALNRVYINIGLFSEEWLL
                     HFNALVGGQPVSPIKITDANKNSSYWNATQEQTPDVALFFLKTARADLLKNAPGGEQY
                     LSKDKAVLDRGKIVFAENCARCHSSKIPAAPLGVNDRIWDQYWAWTKTDDFKKQMTEM
                     VMADDFLTDNYLSTERRIPVTLLQTNACSPLATNALGGNIWDNFSSQTYKDLPSVGKI
                     TVHNPIDGSASEYDMPAGGRGYTRVPSLISLWSTAPFLLNNSVGKFNSSPSVAARMES
                     FNDSIEKMLWPDKRDKDAILGDKVPGFIYRTTDTSYIRVAPGFVSPLLEKMLSWGGWL
                     NRWFPWLFSEEGIEIGPIPKGTPVSLLTNIDLDSRKLDLAKLLLKMKGDLKAVEGKSE
                     KEAAEVFKGLVPDLLKVSKCPDFVVNKGHYFGTALAKDGPGLSDEDKQALIEFLKTF"
     gene            complement(5045..>6521)
                     /locus_tag="VMZ02_00600"
     CDS             complement(5045..>6521)
                     /locus_tag="VMZ02_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUR70450.1"
                     /translation="QGETMTDATRKKTAVKIILALLVVAVALSAAIGWYKFFREEPQP
                     AWVTATPDMRFKYGSIGAENDAGIPYWIFFVLPRMFPEKLPGPGGYASLGVAWEQGQE
                     LPIGFTKKVIGFPRVANNCASCHTASYRKTANENPTFVPTGPNHTLNLQAFFRFLVDC
                     AKDPRFNASEIMREIELVYDLPFLDRLIYRYLLIPITKKRLLEREAQFKWVYHKDFPQ
                     WGRGRDDAMNLTKYFLLKAPMDNTIGPTDMPSVWNLKKYQPEKGMLMNLAGDSHDARS
                     VIIDSALGLLSAEPKNRDEFLQQIDWLVNYLSQIQAPKYPFSINQELADAGKTVFDQN
                     CAACHRSDRTGKRVPVEEVGTDKERLATWSKQAAIDSNKMVKSFGIERKGLVETEPSG
                     YIAAFLDGIWLRAPYLHNGSVPTLRAMLNPAAERPKVFYRGYDVYDPVNIGFVTEGED
                     AQRVGTKFDVSERASGNHGHEFGTSLSAADKDALLEYLKTL"
BASE COUNT         1427 a         1865 c         1785 g         1444 t
ORIGIN      
        1 gagcgacgaa ctggcggtca ccgcggcggc gaggaacgga tgaggcaccg cggccatgcc
       61 gaatttacga acgacctggg aagcgccggc cagcgccgag gcagcgatgg gataccaaag
      121 aaacaccgcc gatcctgcaa gcttcatggc gccgtgacgc catgacaata gcgtgatgcc
      181 actgatgatc atgaatgttg caatgacgat cggcaaggtg atctcctcgc cgagaaagac
      241 gatcgccatg gccgcgctaa acagcggcgt gctcgcgcgg atcggatcgg tgatcgccac
      301 gcccaaggaa tcgatgccct tgtaagtgag caagcgtgtc agcccgggtt gaaaaattcc
      361 gacaccaacg aaaatcatga gcgacgccaa agtcacatgg gccatcggca cgaacaaaaa
      421 agacatcgtc cagagaaaga cgatgttaat cagcaaactg ctgatgacgc cggtcgccgg
      481 gttggaatag cgcagcgcct ttttcgtgaa tatattgtgc accgcgaaca aaaacgcatt
      541 gagcaaagcg taaaactcgg cagggatgga agtcatgatg aagtggaatc gatgaagcag
      601 tgaagtgatg gagtgttggt atcgaaatga ctcgagctat ataggcgcga agtaaaaacc
      661 cgactgattt cttgcgaatt aattccttgc gctagtgaga atcacttcag ccgccttctc
      721 gccgatcata taaatcgcgc tggcgataaa gaaaccggga atgcgcggaa acacagacgc
      781 gtcgacgacg cgcagtccgt tcgtgccgtg cacacggaaa tcgctgctca gaacgccgcc
      841 atggtctcgc gcgccgatgg cgcagctgca cgacgcgtga tgcccccagg cattgtcgcg
      901 cacgaactcc cgcaactcat cgtcggtttg atattgttcg ccgggcaact cttcggcggc
      961 gatcacgcct tgttttttga ggccccgagt catcgagcgg acaaattgaa tcccctcgac
     1021 gaccgcgtcc aaatctttac cttcatcagc gctgccttcg tcgaagtaat tgaagtgaat
     1081 ctggggcggc tggcgtggat cggcagatcg cagagcaacc gtgccggcac ggttacgggt
     1141 gcgcgccttt aaaatgatcc agctcaaata gttctgccgc tcggcgatta atttcgcgta
     1201 gccaggaaaa tagccgcgaa aattgccgac cagtccggca caaaacagat cggggactcg
     1261 atcctgcttt gactgcttaa ccagcgccag cacattgcca ttggtcgtgt agacgccgtc
     1321 ttttcgcgcc agccagtcgt gatactgtga atcgtttttg gcgaattggg cgccggccaa
     1381 catcggccaa ttgtctttca tgcggttgac gacgctgatc tcgtagcggt cctgaagatt
     1441 tttcccgacg ccgtcaagat cgacacgact ctggatccca tgccgaatca attgctcgcg
     1501 cggtccgatg ccggaaagca tcagcagctg cggcgtgttg aaggcgccgc cgcaaagaat
     1561 tacctcgcga cttgcgcgca tccggcgtat ttcgccggcg ctgtcgctga cattggcgtg
     1621 cgcccggtac agccgctcgc ccttgagata ttcgacgcca attgcccgtt gttcaccatc
     1681 gaacaagacg cgggttgcca gggcatgaca ctcgacgtgg agacgatggg gaaacttttt
     1741 tgcaatgctc aacacgcgct cccgactgcc catccgcagg tggtcgcggg tagtcaaagg
     1801 cacgagacgc aatcctgacg cactggccgt aatctggcgc caatcgttgg gatcggcgcg
     1861 ggttttaaga aggccgtgca atcttttcag ccgatcgccg atctccaacg aggcgtcccg
     1921 cgccaactct tccaacaccc ggcgtagccg gcggttgccg agcgtttcca tcggcattgc
     1981 cagttcggtg ctgagccagc cgccaaatcc atgccgcgcg ggattgaaac ccgtcaaacg
     2041 agcgatccag cgcttcaacg gccggtgccg gcagtcttcg agcaactcga aatagcggcg
     2101 catgttactc gcgcgccaag tcgagtctcc cgtcaataaa gcaatgtcat cccagtcctg
     2161 attatttgga tagacaaaga tcatggcgtt gtgtgcggta caaccgccga gcgtgccggc
     2221 gcgcggataa agcacgccgg cgtgatcggg gcaatacttg ctgtctcttc gttggcgctg
     2281 ctcgttggcg taatggcgca cgaagaaatc ccagcgcatc gccggattct ccgcagcgaa
     2341 agcgtgaaac gcaggaacct ggtagtcttc cggcagtcgc gaatcggcga gctcacaggg
     2401 atcgccgccg gcttcgagca gcaacaccga acggcccgcc tcggccaacc gcgccgcgac
     2461 tgtgccaccg cccgctcccg aacccacgac gatgtattcg tactgggctc gcgtagcggt
     2521 ggcttcggac attggaagaa gttttaggtg acggcggaat taaaacgttt tcaagaattc
     2581 gatcaacgct tgtttatctt cgtcgctcaa tcccggccca tctttcgcca acgccgtgcc
     2641 aaagtagtgg cccttgttga cgacgaagtc ggggcactta ctgactttca acaggtccgg
     2701 caccaaaccc ttgaagactt ccgcagcttc tttttcgctt ttgccttcga ctgctttcaa
     2761 atcgcccttc attttcagca gcaacttggc aagatcgagc ttgcggctat cgagatcgat
     2821 attggtcagc agactgaccg gcgtcccttt cggtattgga ccgatctcaa tcccctcttc
     2881 gctaaacagc caggggaacc agcggttgag ccagccgccc cagctgagca ttttctccag
     2941 cagcggtgag acaaatccag gcgcgactct gatatagctg gtgtccgtcg tgcgataaat
     3001 aaagcccggc actttgtcgc caaggatcgc atctttgtcg cgcttatcgg gccaaagcat
     3061 tttctcgatg gagtcgttga atgactccat gcgcgctgcg accgacgggc tcgaattaaa
     3121 tttaccgact gaattgttca agagaaacgg cgccgtcgac cacaagctga tcaaggacgg
     3181 cacccgcgta tagccgcgtc cccctgcagg catgtcatat tccgaggcac tgccgtcgat
     3241 cgggttatgc acggttattt tgccgacgga gggcagatcc ttataagttt gcgaggagaa
     3301 gttgtcccaa atattgccgc ccagcgcatt ggtcgccagc ggactgcagg cgttggtctg
     3361 caacagcgtc accggaatgc gccgttcggt ggagagatag ttgtcggtga gaaaatcatc
     3421 ggccattacc atttcggtca tttgcttctt aaagtcgtcg gtcttggtcc aggcccagta
     3481 ctgatcccaa atcctatcgt tcacgcccaa cggcgccgcc ggaattttac tggagtggca
     3541 gcgcgcgcag ttttcggcga agacgatctt gccgcgatcc agcaccgctt tgtccttgct
     3601 cagatactgt tcgccgcccg gcgcattctt caacaggtcc gccctggctg ttttgagaaa
     3661 gaacagcgcg acgtccggcg tctgctcctg tgtggcgttc cagtagctgg agtttttgtt
     3721 cgcgtcggtg attttgatcg gcgacacggg ctgaccgccc accagcgcgt tgaaatggag
     3781 cagccactcc tcgctgaaca gaccgatgtt gatgtagacg cggttcaacg ctccgagaat
     3841 gccgacggaa tccgcgccgt ctttcaacac atgtgccgtc cacactttat ccggctcttt
     3901 ataatactgc gccagcgcgg cagtgcgcgg gtattgttga aactgcttgt tgtcgaggcc
     3961 gccgcccgcg agtttctcct cgccccagcg ctcggcgatc ttcaagcgcg cgccgacgtt
     4021 ataaatcgcg ttcatggtgc gcggattgtt gatatagtcg ctggaaatga acgaggtatc
     4081 gagcgttccg ggcaacgatg tgtggatcag ttggtagata aaattcttcg agtccctttc
     4141 ccaaaacagc acacggtcga cccagaagta ttgcgagccg gggttggagt tgatgttttc
     4201 ccattgggga ttttccgggt cgtccggagg tttaaccgga ctcggtccaa catggcagaa
     4261 accgcatgcc atgccgacgc ggtacggacg aactaaattt ttgtcgttgt agtatttcgg
     4321 gtcggtgtaa aatctttccg catcccattt tttcgctgcc gcttcgtcga aatccggatt
     4381 gggaaacaag cgcaaaccaa gaatccccgt agcgtacccg tagtaggaac ccaccggcaa
     4441 gtttttgcca cgcgcgccga ttctcacgcc ggggtacttt tgctcgtttt cgaagggatc
     4501 cgcggggcaa tctttactgc gcgtatctag ccacaggccg aatcgatcgc gattcggcgc
     4561 cttggccttt tcaaagcagg gctcgttggt aacaccgaga tattcccagc gcttgtcgcg
     4621 gctcagatgc ggcaacttgg ggtgtgagga aataatcttc agtaaatcga cgacgccgaa
     4681 actcttgttc accaggtgat cccaaaaacg gtcgttgccg gcgctccaaa ccagccagtt
     4741 attgcggcct ttgacttcag ccacgctgag ctcgatgccg ccatccattt gttgaaaata
     4801 atcctcatcg gcggcgggaa aactttgtgc ggctcgtccc acggttaaag cttcgtcctt
     4861 tacggtcccg aagtcgggtt ttttcttacc gcacccgcct aacgccaacc aagcgacaag
     4921 catcaccaca ctgctgcgaa aacgcagatc cattgtctat acccctccaa aaacaactgc
     4981 tccggcaacg atcagtaacg acactgaccc ggtcggataa ttttgcgaca actcaccaat
     5041 ggcgttagag agttttcaga tactccagca gcgcatcttt atctgctgcc gatagacttg
     5101 tgccgaattc atggccgtga ttgccactgg cgcgttcact cacatcgaac ttagttccca
     5161 ccctttgcgc gtcctcgccc tccgtgacaa agccaatatt caccggatcg taaacatcgt
     5221 agccgcgata aaaaaccttg ggccgctcgg ccgccggatt gagcatggcg cgcaacgtcg
     5281 gcaccgaacc attgtgcagg tacggcgcgc gcagccaaat gccgtcgaga aaggccgcga
     5341 tgtaaccgct cggctcggtt tcgacgagtc cttttctctc gatgccaaaa ctcttcacca
     5401 tcttgttgga atcgattgcc gcctgcttgc tccaagtcgc taaccgctcc ttgtcggtgc
     5461 cgacttcctc gaccggcacc cgcttgccgg tacggtcgct gcggtgacag gcggcacagt
     5521 tctggtcaaa gacggttttg ccggcgtcgg cgagttcttg attaatcgag aatggatatt
     5581 tcggcgcttg aatctggctt aaataattta ccagccaatc tatttgctgg agaaattcgt
     5641 cgcggttctt cggctcggca ctgaggagac cgagcgcgga atcgattatc accgagcgcg
     5701 cgtcatggct gtcgccggcc agattcatca acatgccctt ctcgggctgg tattttttga
     5761 gattccacac cgacggcatg tccgtaggcc caatggtgtt gtccatcggc gctttgagca
     5821 gaaagtattt ggtcagattc atcgcgtcgt cgcgaccgcg gccccactgg gggaaatcct
     5881 tgtggtaaac ccatttgaac tgcgcttccc gttcaagcaa gcgtttcttg gtgatcggga
     5941 tgagtaaata gcgataaatt aatcgatcga gaaacggcaa gtcgtagaca agttcgatct
     6001 cgcgcatgat ctcactggca ttgaaacgcg gatctttcgc gcagtcgacg aggaagcgaa
     6061 agaatgcctg cagattaagc gtatgattgg gtccggtggg gacgaaagtc ggattttcat
     6121 tagcggtctt gcgataactt gcggtatggc aggaggcgca gttattggcg acacgcggga
     6181 aaccgatgac cttcttggtg aagccaatcg gtagctcttg cccttgctcc cacgccactc
     6241 ccaacgaagc atagccgcca ggacccggta atttctccgg aaacatccgt ggcagtacga
     6301 agaaaatcca ataaggaatg ccggcatcgt tctccgcacc aatggagccg tatttaaagc
     6361 gcatgtcagg cgtcgccgtc acccaagcgg gttgcggttc ttcgcggaaa aacttgtacc
     6421 agccgatcgc agcgctgaga gctaccgcca cgacaagcag cgccaagatg atcttcacag
     6481 cagttttttt cctcgtggcg tcagtcatcg tctctccttg c
//
LOCUS       DATLCH010000023         7743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8235034, whole genome shotgun sequence.
ACCESSION   DATLCH010000023 DATLCH010000000
VERSION     DATLCH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8235034"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VMZ02_00605"
     CDS             <1..886
                     /locus_tag="VMZ02_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70451.1"
                     /translation="NTALSRVFAVDTLGLERIKHVFQPEISYLFVPHVNQDKIPIMDD
                     IDRINRRNVLTFAVNNRLWGKSPSPITNQSLDSGTEYLSPVATGDVREFASFRMALSY
                     DIDKERKGGDSLSDLDLRLRLTPAPYLEFLLDGGMNPGPWDFTQVLASVGITDPRPLF
                     RRSPDADFNRPNSFSLSYHFLRRGPNSFLADDANANVDSCIDHPNDPDCTKNTVGNIK
                     NNLLYHVTDNLLFNFNSTYDVRDGRFIGFRTTSKFLSFCECWTATFGVSRSVNPKKTS
                     FSVDFNLLGLGSPKSSLK"
     gene            1081..1461
                     /locus_tag="VMZ02_00610"
     CDS             1081..1461
                     /locus_tag="VMZ02_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70452.1"
                     /translation="MADRLTHPDKVIDKPWGYELIWAHTERYVGKVLHINKGESLSYQ
                     FHNVKDETIRLLSGVLELDIEKDGRQEKIRLGVGESLHIVPLMKHRMIAVEDCDVLEV
                     STPELDDVVRLEDRYGRADSPAHK"
     gene            1479..3179
                     /gene="gspE"
                     /locus_tag="VMZ02_00615"
     CDS             1479..3179
                     /gene="gspE"
                     /locus_tag="VMZ02_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HUR70453.1"
                     /translation="METQATFADRLVERQMLSSEEMERVRKLQGEQQAPLSRLVVELG
                     FLSEEDLLPLMRDHFDLPLMSLRDVPNTALPIDLPASIGDFFKLARMVPVKIEGRELI
                     VALADPTELSRLHSIELATGLRVRAVLAKEKEIAARIDTLYGGSYSPDGAAEQSAQAF
                     DGIAEDEDVAHLRDMASEVPVIRLVNQMLVRALESRASDVHIEPFENQLKVRYRIDGI
                     LHEVESPPRQLKGAVISRLKILAQLNIAERRLPQDGRIKTRLGGKDIDLRIATVPTLY
                     GESVVIRLLERGQIFTQLETLGFPPAQLARFNEMIVKPHGMILVTGPTGSGKTTTLYG
                     ALQKINDPGKKIITIEDPVEYQLGGVNQIHVKPQIGLTFANGLRSIVRQDPDVIMVGE
                     IRDAETAEIAVQAALTGHLVFSTLHTNDAAGAISRLLEMGVQDYLLSSSLLGVLAQRL
                     VRRLCPNCRKDVPFTGLEGVAPEMALANSEALHTIWEAIGCETCSGTGYLGRVGVFEL
                     LPVTADICKLINQKADANAIRNLAAQQGMRLLREDAWDKVRQGMTTLAEVVRVTREEG
                     "
     gene            3231..4448
                     /locus_tag="VMZ02_00620"
     CDS             3231..4448
                     /locus_tag="VMZ02_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HUR70454.1"
                     /translation="MAFYQYRAADQAGKVVEGVMEADAEQSVVSRLREMGCVPLRIAM
                     PSERAVGAIQQQRPLFEKRKVSQQQLLQFTRELATLLTAGLPLDRSLSILGNLFEGAE
                     FSKVLSSLVEAVRAGKSLAAAMGEHPQVFPRIYVNMIRAGEAGGILESVLRYLTEYLE
                     STTALKEEIKSALIYPVILASAAGASLIVLFVFVIPKFAALFHENRKAIPWITKMVID
                     FSQLLSQYGWLMLLVVAAAAAGAIFYVKSPEGRSEWDRLCLKAWLIGDLVRKFETARF
                     SRTLSALLKGGVPLLEALGTVQGVVGNRLLARAIGQVQVRVREGKGMARPLSESGLFP
                     ILALNMIAVGEESGKLDGMLTEVASFYDQEVRRSTKRLTALLEPILILGMGLIIAVVV
                     ISMLMAIFSINDF"
     gene            4472..4885
                     /gene="gspG"
                     /locus_tag="VMZ02_00625"
     CDS             4472..4885
                     /gene="gspG"
                     /locus_tag="VMZ02_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HUR70455.1"
                     /translation="MKNQAGFTLIELIVVVIIIGLLAGLVVPQFMKQEERATAKTAKA
                     QIELFGTALDTFRLDVGRYPTSQEGLAALMQKPGGADRWDGPYLKKDLPPDPWGKPYV
                     YKSPGDHGVYDIISYGADGVAGGDGNNRDITSWER"
     gene            4860..5327
                     /locus_tag="VMZ02_00630"
     CDS             4860..5327
                     /locus_tag="VMZ02_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUR70456.1"
                     /translation="MTLQAGKDSRGFSLLELVLVLVVMGISMAIVVPNIGRRLQEREV
                     RSSALGLAALARDLRSRAVLDGIVQQLVVSMPHNTYFVPKVREIHLPKDVRITSIEGG
                     EAVERDTKRFYFFPNGSSLGGQIVIGDDANSISYNIRMEPLTGKIEVARGDHF"
     gene            5336..5734
                     /locus_tag="VMZ02_00635"
     CDS             5336..5734
                     /locus_tag="VMZ02_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HUR70457.1"
                     /translation="MRQSTAGFTLLEVMVAMAIVGFGVVTLLEIFSLGLRLGSRSNVH
                     TEAVAYSARVMDELLARPKLRDGSESGTLASTNRWRAQIQTLRDATPSLNLGSNWELK
                     EIGVEMTVPEGDQERRVELKTLRLARKGNP"
     gene            5731..6393
                     /locus_tag="VMZ02_00640"
     CDS             5731..6393
                     /locus_tag="VMZ02_00640"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HUR70458.1"
                     /translation="MSRRSAAIVRPGSPSKVRVLGAAGFTLIEVVLAIAIFALMGGVL
                     YGAFSLGHTAVEKSEKNFSRSQQTRSIGDLLATYIRSGYPYRESIQEQSIFFEGERDS
                     MTFVSAYSHGMGGRGMATVAIEKEETDTGRMSFRLTETAPVRTEGGDGGQRHSLMIQE
                     GVRDFQLAYLDGEGDTENWEERWDGKERRRLPRAVRISFIDAEGKEVRWIFPFMLVVL
                     SQ"
     gene            6390..7406
                     /locus_tag="VMZ02_00645"
     CDS             6390..7406
                     /locus_tag="VMZ02_00645"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70459.1"
                     /translation="MTCVFRYNFKGRFDNQRGVALIIVLWIFIFLFVVAFDFSASVRE
                     EAAATHRFSDETQGYYLALAGFQRGLYNFIQQAGGGNPQTDIKQNDLFDGTWREEKLG
                     SGMFRVRLVDEGGKINLNRVDEQILRRVFTNLGMEDSAADILVDSIMDWRDPDDLHRT
                     NGAESDYYGSLSPAYSAKNGPLDSVEDLLWIRGMTPAMFYGTVNDGNPQVERAPRVGL
                     RDVFTIDSPIDRVNLRTATAEVIHALTGIAMERCRSFVEERKRLADKTLNDLLPLLGI
                     GSGDPALQTFIFTNPSVIAVDAEGRPQDSRVARRVKGVVRLGGAQGFEILRWLDREAG
                     LSQP"
     gene            7458..>7743
                     /locus_tag="VMZ02_00650"
     CDS             7458..>7743
                     /locus_tag="VMZ02_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70460.1"
                     /translation="MALGQRLQDLSRADFLRSVGLYVMQDRLIMVRLRKSLLTVSLLD
                     EQERELPQSEARPAIADITGWVAEDVKEIALKAENDSRERSLRQAMVSLLP"
BASE COUNT         1707 a         1987 c         2234 g         1815 t
ORIGIN      
        1 caatacggcg ttgagccgag tcttcgctgt cgataccctg ggtttagagc gaatcaagca
       61 tgtttttcaa cctgagatca gttacctttt cgtcccgcac gttaatcagg acaaaattcc
      121 catcatggac gacatcgatc gcatcaatcg ccgcaacgtg cttacctttg ctgtcaacaa
      181 tcgcctgtgg ggaaaatccc ccagtcccat caccaatcaa agcttggaca gcggtacaga
      241 atatcttagc ccggtggcga ctggcgatgt gcgggaattc gcatcattcc ggatggctct
      301 gtcctacgac atcgacaagg agcgcaaagg cggcgacagc ctgtccgatc tcgatctgcg
      361 tttgcgtttg acgccggcgc cgtatctcga gtttctcttg gacggcggca tgaaccctgg
      421 accgtgggac ttcacccaag tgctcgctag cgtcggcatc acagatcccc ggcctttgtt
      481 ccggcgctca cctgacgccg atttcaaccg gccgaattcc tttagcttga gttaccactt
      541 cttgcgccgt gggccgaata gctttcttgc cgacgatgcc aatgccaacg tggattcatg
      601 catagaccat ccgaatgatc ctgattgcac gaaaaacacc gtagggaaca tcaaaaacaa
      661 tcttttatac catgttaccg acaatctgct gttcaatttt aacagcacat acgacgttcg
      721 ggatggccgc ttcatcggct tccggacgac gagcaagttt ctctcatttt gtgaatgctg
      781 gacagcgacg tttggtgttt cgcgcagcgt taacccgaag aaaaccagct tcagtgtcga
      841 tttcaatcta ctcggactag gtagtccgaa aagttcattg aaataaccac cacatccgct
      901 gccccgctaa ttgcaagatc ccgtttgcct gggcgggcgg tgtctgcggc cagcttcgat
      961 tttggcaata aggcggcagc aacattcgtt gacaaatgct gtcccatcaa tgtcggtgta
     1021 attacttgga agcgagtttt gtttgccgca ttggcttaat ctggtatcat tgccctcaat
     1081 atggcagacc gattgactca tcccgacaaa gttattgata aaccgtgggg ttatgaattg
     1141 atttgggctc ataccgagcg ctatgtcgga aaggtgctgc acatcaacaa aggcgaatcc
     1201 ctcagttatc agtttcacaa cgtgaaagac gaaacaattc ggctgctctc tggcgtgctc
     1261 gagttggata tagaaaagga cggtcggcaa gagaagattc gtttaggagt tggcgagagt
     1321 ctgcatatcg tgccgctgat gaaacatcgc atgatcgccg ttgaggattg cgatgttctg
     1381 gaagtttcga cgccggaact cgatgatgtc gtgcgcttgg aagaccgcta tgggagagcg
     1441 gattcgccgg cgcacaaata atttcactgg aaattctcat ggaaacccaa gcgactttcg
     1501 cagaccgtct agttgagcgg caaatgctct ccagcgagga gatggagcga gtgcgtaagc
     1561 tccaaggaga gcaacaagcg ccactcagcc ggctggtggt cgagctaggc tttctgtcgg
     1621 aagaagatct ccttccgctc atgcgcgatc actttgatct gccattgatg tcgctacgcg
     1681 acgtccccaa tacggccttg ccgatcgact tgcccgcgag tatcggcgat ttttttaagc
     1741 tcgcgcgtat ggtgccggtt aaaattgagg gccgcgaatt gattgtcgcc ctggccgatc
     1801 cgacggaatt atcgcgcttg cattccatcg aattggcgac cgggttacgg gtcagagcgg
     1861 tgctcgccaa agaaaaagag attgccgcgc ggatcgatac tctgtacggc ggcagttaca
     1921 gccccgatgg ggcggccgaa cagtcggcgc aagcatttga cggtatagcc gaagacgaag
     1981 acgtcgccca tctgcgcgat atggcatcag aagtaccggt aatacggctg gtcaaccaga
     2041 tgctggtgcg cgccctcgaa agtcgggcat cggacgtgca catagagcct tttgaaaatc
     2101 agttgaaggt acgttaccgc atcgacggta ttttacacga agtcgagtcg ccgccgcgac
     2161 agttgaaggg cgcggttatc tcgcgcctga aaattctcgc acaactcaac atcgccgagc
     2221 gaagactgcc gcaggatggc cggatcaaga ctcgattggg cggtaaggac attgatttgc
     2281 gtatcgcgac agtgccgaca ctctacgggg agagcgtcgt catccgctta ctggagcgcg
     2341 gacagatttt cacacaattg gagactctgg gttttccccc ggcgcaattg gctcgtttta
     2401 acgagatgat cgtcaaaccg catggcatga tcttggtcac tggaccgacc ggcagcggca
     2461 aaactaccac gctctatggc gcgctgcaga agatcaatga tccgggaaag aaaatcatta
     2521 ccatcgaaga tcctgtcgag tatcagttgg gcggcgtcaa tcagattcac gttaaaccgc
     2581 agatcggatt gacgttcgcc aacgggctgc gctctatcgt gcgccaagac cccgacgtta
     2641 tcatggtcgg cgaaattcgc gatgccgaga ccgctgagat cgcagttcag gccgcgctca
     2701 ccggccactt ggtattttcc acgttgcata ccaatgatgc ggccggggca atttcacggc
     2761 ttttggaaat gggtgtgcag gattatttac tgtcgtcgtc gttgcttggc gtcttggcgc
     2821 agcgattggt gcgccgcctc tgtccaaatt gtcgcaaaga tgtgccgttt accgggctgg
     2881 agggcgtcgc gcccgaaatg gcgctcgcca atagcgaagc cttgcatacg atttgggaag
     2941 cgatcggttg cgagacttgt agtgggactg gttatctcgg ccgtgttggc gtctttgagc
     3001 tgctgccagt cactgcggat atatgcaagt tgatcaacca aaaggccgat gccaatgcca
     3061 tccgcaatct ggccgctcag caaggcatgc gactgctccg tgaggatgcc tgggataaag
     3121 tgcgccaggg aatgacgact ttggccgagg ttgtgcgcgt gacccgcgaa gagggatagc
     3181 ctcaaactat tcaaacactc ctgtcgttcg atcgtgcgta aacactaatc atggcgtttt
     3241 accaatatag ggccgctgac caggccggta aagtcgtcga gggcgtgatg gaggccgatg
     3301 ccgagcagag cgtggtatcg cgcttgcgcg aaatgggctg tgtccctctg cgcatcgcca
     3361 tgccgtctga acgtgcggtc ggcgccatac agcaacagcg gccgctcttt gaaaaacgca
     3421 aagtcagcca gcagcagcta ctccagttta cccgagaact cgccacattg ctgaccgctg
     3481 ggttgccgct ggatcgcagc ttatcaatac tcggtaatct ttttgaagga gctgaattca
     3541 gcaaagtgct gagttcgctt gttgaagcgg tgcgcgccgg caagtccctg gccgcagcaa
     3601 tgggcgagca cccgcaagtt tttccgcgca tttacgttaa catgattcgt gccggtgagg
     3661 ccggcggtat tttggaaagc gtcctgcgct acctcactga atatttggag agcactacgg
     3721 cgctcaagga agagatcaag tcggcattga tttacccggt catcctcgct agcgcagcgg
     3781 gcgcttcact gatcgtcttg tttgtgttcg tgatccctaa gttcgcagct ttgtttcatg
     3841 agaatcgcaa agcgatccct tggattacaa aaatggtaat cgacttcagc caactattgt
     3901 cccaatatgg ctggctgatg ttattagttg tggctgccgc tgccgcgggg gcgatttttt
     3961 acgtgaagag tccggagggc cgaagcgaat gggatcgcct ctgtttgaag gcctggctca
     4021 ttggtgacct ggtgcgaaaa tttgaaaccg cgcgtttctc gcgcacatta tcggcattgc
     4081 ttaaaggcgg cgtccctttg cttgaagcat tgggcacggt tcagggggta gtcggtaatc
     4141 ggcttctagc cagggccatc ggccaggtgc aggttcgggt gcgcgaaggc aagggcatgg
     4201 cacggccgtt gagcgagagc ggtctttttc ctattttggc gcttaacatg attgccgtcg
     4261 gcgaagagtc gggaaagttg gatggtatgt tgaccgaagt cgcgtcgttc tatgaccagg
     4321 aagtcagacg ttcgaccaaa agactaaccg ccctgctcga gccgatttta attttgggga
     4381 tgggcctgat catcgcagtt gtcgttatct ccatgttgat ggcaattttt agcatcaacg
     4441 atttctaata acttatcatt ttagcagagc tatgaagaat caagcgggct ttacacttat
     4501 tgaattgatt gttgtggtca tcatcatcgg cttgttggcc gggctagtgg tgccccagtt
     4561 catgaaacag gaagagcgag cgacggccaa aacggcgaaa gcacagatcg agttatttgg
     4621 cacggcgctc gatacctttc gcctcgacgt cggccgttat ccgacgagcc aagaaggctt
     4681 ggcagctttg atgcaaaaac ctggcggtgc tgaccgttgg gacggccctt atttgaagaa
     4741 agacctgccg cccgatccct ggggcaagcc gtacgtctat aaaagtccgg gagatcacgg
     4801 tgtctatgac atcatttcct atggcgcgga cggcgtcgcc ggaggcgacg gcaataatcg
     4861 tgacattaca agctgggaaa gatagccgag ggttctcact ccttgagctc gtgctggtgc
     4921 tggtcgtcat gggcatcagc atggcaatcg ttgtgccgaa catcggcagg cgcctgcaag
     4981 agcgggaggt gcgcagttcc gcgcttggcc ttgctgcgtt agcgcgcgac ctccgcagcc
     5041 gcgccgtact cgacggcatt gtccagcaat tggttgtcag catgccgcat aacacctatt
     5101 tcgttccgaa ggtacgcgaa attcatcttc ccaaagatgt gcgcattacg tcaatcgaag
     5161 gcggcgaagc agtggagcgg gacaccaagc gattctattt tttccccaac ggcagcagcc
     5221 taggcgggca gattgttatc ggcgatgacg ccaattcgat ttcttataat attcggatgg
     5281 aaccgctcac tggtaagatc gaggtcgccc gcggcgacca tttctgaaag ctacgatgcg
     5341 acagagcaca gccggattta ctttgcttga ggtcatggtc gccatggcca ttgtcggttt
     5401 cggtgtcgtg accttgctgg aaattttttc cctcgggcta cgtctgggat cgcgcagcaa
     5461 cgtccacacc gaggcggtcg cttacagtgc gcgggtcatg gacgagttac tcgcacgccc
     5521 taagctgcgt gacggctccg agagcggtac gctggcgtcg actaatcggt ggcgcgcgca
     5581 aatacagacg ctgcgcgatg ctacgccctc actcaatctt ggcagcaact gggaactcaa
     5641 ggaaattggc gtcgagatga cggtacccga aggcgatcaa gaacgacgag tcgagctcaa
     5701 gaccctgcgg ctcgcgcgca aaggcaatcc atgagtcgac gatctgccgc gattgtccgg
     5761 ccgggaagcc cgtcaaaggt gcgggttctt ggtgctgccg gctttactct aatcgaagtg
     5821 gtcttggcga ttgcgatttt cgcgctaatg ggtggtgtgc tctacggcgc gttttctctc
     5881 ggccataccg cggtagaaaa atcggagaaa aattttagtc gaagccaaca gacccgatcc
     5941 atcggcgatc ttctggcgac gtatatccgt tctggctacc cctaccggga gtcgattcaa
     6001 gagcaatcga tttttttcga aggcgagcgc gacagtatga ctttcgtctc ggcctactcg
     6061 cacggcatgg gcgggcgggg tatggcgacg gtcgcgatag agaaggaaga gactgacact
     6121 ggccgaatga gcttccggct tacggagact gcgccggttc gcacagaagg cggcgatggc
     6181 gggcagcgtc atagcttaat gatccaggaa ggagttcgcg attttcaact cgcttatctc
     6241 gacggcgagg gtgatacgga aaactgggag gaacgctggg atggtaaaga acgccgccgc
     6301 ttgccgcgcg cggtacgtat ttcgttcatt gatgccgagg gcaaagaagt ccgctggatt
     6361 tttccgttca tgctggttgt gctttcgcaa tgacgtgcgt ttttcggtat aatttcaaag
     6421 ggcgattcga caaccagcgc ggtgtggcgt tgatcatcgt tctgtggata tttatttttc
     6481 tttttgttgt cgctttcgat tttagcgctt cggttcgtga agaagcggcg gcaacgcatc
     6541 gatttagtga tgagacgcaa ggctactacc tcgcattggc cggtttccag cgtggcttgt
     6601 acaatttcat tcagcaggcc ggtggcggca acccgcagac tgatataaag cagaacgatt
     6661 tgttcgatgg tacttggcgc gaggagaagc ttggcagcgg aatgtttcgt gtacggctgg
     6721 tcgacgaggg cggcaaaata aacctgaacc gcgtcgatga acagatcttg cgccgggtat
     6781 ttacaaactt gggaatggaa gactccgcgg cggatatcct cgtcgactcg attatggact
     6841 ggcgcgatcc cgacgatctc catcgcacca acggtgcgga gagcgactat tatggttcgc
     6901 tctcacccgc ttactcggct aaaaatgggc cactcgatag cgttgaggac ctattgtgga
     6961 ttcgcggcat gacgccggcc atgttttacg gtacagtcaa cgacggcaat ccccaggttg
     7021 agcgtgcgcc gcgagtggga ttacgagatg tcttcaccat cgatagtccc atcgacaggg
     7081 tcaatttgcg taccgcgaca gcggaagtga ttcacgcctt gaccggcatc gcgatggaaa
     7141 ggtgccggag ctttgttgag gaacgcaagc gtttggcgga caagacactg aacgacttgc
     7201 tgccgctctt ggggatcgga tcgggtgatc cggcattgca gacgtttatt tttaccaatc
     7261 cgtcggtgat tgccgttgac gccgaaggcc gacctcaaga ttcccgcgtg gcgcgccgag
     7321 tcaaaggtgt cgttcggttg ggcggcgccc aggggtttga gatattgcgt tggctcgatc
     7381 gtgaggccgg actatcacaa ccgtgaagtt tgttgtaaac tagtatcgtt aatcaaaaat
     7441 aagacccgca agttttcatg gctttaggac agcgccttca agatttatca cgcgccgact
     7501 ttctgcgaag cgtcggactt tacgtgatgc aggaccgcct gataatggtg cggctgcgca
     7561 aaagcttact caccgtatcg ctgctcgatg agcaggaacg cgagctacct cagagcgaag
     7621 ctcgaccagc tattgccgat atcaccgggt gggtcgccga agacgtgaaa gagatcgcgc
     7681 tgaaagccga aaacgattcg cgtgagcgca gcttgcgcca agcgatggtt tctttgttgc
     7741 ccc
//
LOCUS       DATLCH010000024         7109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9730448, whole genome shotgun sequence.
ACCESSION   DATLCH010000024 DATLCH010000000
VERSION     DATLCH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9730448"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..646
                     /locus_tag="VMZ02_00655"
     CDS             290..646
                     /locus_tag="VMZ02_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR70461.1"
                     /translation="MNYQLEHVAIYCKDLNATIEFYEKFFGGQPTAIRKGDAGYAFCF
                     MRIAGSPAIQLMESATGVGVHHYGFITDDIDQVCREFKEKGAAILRENRDTAGKLTTI
                     FVKDPNGLEMEVRGPR"
     gene            730..1338
                     /locus_tag="VMZ02_00660"
     CDS             730..1338
                     /locus_tag="VMZ02_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HUR70462.1"
                     /translation="MARANRELELFAVPAKVPEGFFYRQDFISQAEEHQLIREIEKLQ
                     LTPFKYYQFIGKRRTASFGWEYEFGSSEITTAAVLPAFLLPIRLRAGELFNMDPNSLL
                     QASIIEYSIGSPIGWHRDVPHFGEVVGISLGAPCRMRFRKYSRNRAKLDRDEILTIEL
                     QPRSIYLLSGAARETWQHSIPPVRELRYSIVMRTVRAKLTNS"
     gene            complement(1436..2470)
                     /locus_tag="VMZ02_00665"
     CDS             complement(1436..2470)
                     /locus_tag="VMZ02_00665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR70463.1"
                     /translation="MKRLVCACALLFVLSWIAEVNAQAPFYQGKTIRIIVGNLPGDAY
                     DLWARIFAQYMGKYIAGNPTFIVQNMTGAGGVVAANYVYSVAKPDGLTLGTFGPSMYF
                     DQLARRPEVRFDWAKFTWIGTPEQTEFILVMRTDTPYQSIEDVRKAAQPPKCGATGTS
                     TSGYYMPKFLDEGLGIKFNIVSGYPGGGEIDLGIERGELHCRNLTISTYFAREPFLTW
                     HKKGFVRPILQTGKKRDPLVPDVPSIYELMDQYKTTEAIRRVATVLLAPAVFGRPLVA
                     TPGVPPDRVKILRDAYERSLKDPALLEDLKKRRWAVDAIGGEELAKLAKEVVSQPPDV
                     VDRMMKLLGN"
     gene            2746..3765
                     /locus_tag="VMZ02_00670"
     CDS             2746..3765
                     /locus_tag="VMZ02_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70464.1"
                     /translation="MHGIAKHWIIYARVFFTLTLAIVSAAQAQDKLLISYGGHNETVG
                     PMWVAADRGIFKRYNLDVNMLQVRNGQISLTAVMAGDVQAFWPAVSSVISGVGAGAKL
                     GCVASPFNKIARELMVRPEIDSLAALREKIFGVQSIGGGLWLQTMIVLDRLGVDADKY
                     KLQMRVIGDEPTILQALLASNVDAAVITQASAAVAKRAGLRSLANTMELKVPYQGVGI
                     CTRSDRIVNSPDLIMRLTKGMVEGVVYIHSPRNKRDVMEVLKKHLRLATDQDAETSYN
                     ALRTVSSLDLAPDPEAWKNIQKYVARVTPKVAQLDIQQIINGNFVKTLEETGFIAETK
                     RRYGL"
     gene            3879..4751
                     /locus_tag="VMZ02_00675"
     CDS             3879..4751
                     /locus_tag="VMZ02_00675"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUR70465.1"
                     /translation="MTEDLQANLRVTGDPRLAEEWELVLLTQGLSPSLRRTPAGMILS
                     VPESERAQAFASLAAYDRENRKPATHVERLDSGNLIAGTAVGLMLLLFYSITVQWSPG
                     LSWLARGSADAERILQGELWRAVTALTLHADIAHVLSNAVAIALFFSALSSIVGGGVA
                     GALVVFAGAGGNLATALLHGSSFVSVGASTAVFAAVGLLGSLGVTRRRRSTLSWRRAW
                     IPLAAALALLGMLGSSGQRVDIWGHLFGLLVGAGLGLPVGLTAPRVLGLLSQWACGTA
                     AVVTVIYCWMLAFR"
     gene            complement(4951..5232)
                     /locus_tag="VMZ02_00680"
     CDS             complement(4951..5232)
                     /locus_tag="VMZ02_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70466.1"
                     /translation="MNTSVSITTPAAITSPYTQTAPAKKPYKSVPENYRVLSETKYHK
                     VVADLEAILLDISTDGVGINTPDGRISTTTLQSWASSLRNALETLSKAQ"
     gene            5556..>7109
                     /gene="ftsH"
                     /locus_tag="VMZ02_00685"
     CDS             5556..>7109
                     /gene="ftsH"
                     /locus_tag="VMZ02_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HUR70467.1"
                     /translation="MSLSLKQMAIGLGVVLVCFTIFFTVNKQYRPDTDIIFSEFIAAV
                     ERGDVVEVMIEGHHIEGQLKNGEQFKTYTPDDPQLVKSLREKNVKVAAKAEGASPWYV
                     TLLVSWLPILLLIGVWIFFMRQMKVGGGKAMSFGKSRARLLTENQHRITFSDVAGVDE
                     AKDDLQEIIEFLKDPKKFTKLGGRIPKGCLLVGAPGTGKTLLARAIAGEAGVPFFSIS
                     GSDFVEMFVGVGASRVRDLFVQGKKKAPCIIFIDEIDAVGRHRGAGFGGGNDEREQTL
                     NQLLVEMDGFESNDGVILIAATNRPDVLDPALLRPGRFDRHVVVPRPDIAGRVGILQV
                     HSRKVPLAADVDVNLLARSTPGLTGADLENLVNEAALLAARNDQEKVNMLDLELAKDK
                     VMMGAERRSMIISDEEKRTTAYHEAGHALVAKLLPGADPVHKVTIIPRGMALGLTQQL
                     PEDEKYTYASKYLLNNLVILFGGRVAEELVFEQITTGAGNDIEKATDLARRMVCEWGM
                     SEKLGPMTFG"
BASE COUNT         1592 a         1876 c         2035 g         1606 t
ORIGIN      
        1 ttatttttcc tctactggtg ctaagatttt ccttggcggc acgggcacac cgccgctaat
       61 gaaaaagtag ttgattaaaa ctaggctggg ctgggcgtgc cggcgccgtc gatcattgaa
      121 tcagcagagc aacgcggtct agatctatct gtagattggc acgcgccagt aatcgtcaaa
      181 ccgaaagagc aggccatttg tttgctagtt aaggaagcac gactgtggcg cttgacgggc
      241 ggaggcaatc ccgaataggt tggtacgata ttctttggct ggaggaatga tgaactacca
      301 attggaacat gtggcaattt actgcaaaga cctaaacgcg acgatcgaat tctacgaaaa
      361 gtttttcggc ggccagccga cagcgatcag aaaaggcgat gccggttatg cgttttgttt
      421 catgcgcatc gccggctcgc cggctattca actgatggaa tcagcgaccg gcgtcggcgt
      481 ccatcactac ggctttatca ctgacgacat cgaccaggtg tgccgagaat tcaaggagaa
      541 gggcgcggca atcctgcgcg agaaccgcga tacggccggg aaactcacga cgattttcgt
      601 caaagatcca aacggattag agatggaggt tcgagggccg cgttgattgt gcgccaagca
      661 tgctcatgcc cacatcacaa attcttggcg gccaattaat ttttgcgcgg gtcatggcat
      721 aataatgtta tggcgcgagc aaatagggag ttagagcttt ttgcagtccc ggcgaaggtt
      781 cccgaggggt tcttttatcg ccaagatttt atctcgcagg ccgaagagca ccaactcatc
      841 cgggagattg agaaacttca gctcacgccg tttaaatatt atcaatttat cggcaagcgg
      901 cgaacggcga gctttggttg ggagtacgag ttcggatcca gtgaaattac aacagctgcg
      961 gtcctgccgg cgtttctctt accgatccgt ttacgggccg gagaactctt caacatggat
     1021 ccgaacagcc tactccaagc atccattatt gaatattcaa tcggctcgcc gatcggctgg
     1081 catcgggatg taccacattt tggcgaggtt gtcggcatct cccttggggc tccatgccgg
     1141 atgaggtttc gcaaatatag ccgcaaccga gcgaaactcg atcgtgacga aatcttgacc
     1201 atagaactac aaccgcgttc gatctatttg ctgagtggcg ctgcgcgcga gacctggcag
     1261 cactcgatac cgccggtgag agagctacgc tactcgatcg tcatgaggac tgtgcgcgca
     1321 aaactcacga actcatgaga cggaaaccgt catcgcggac ttgctgcagt cggagaatcg
     1381 cggcattcaa gaagcgcaac aaggagcagt tctgaagcac cgacagcggt tgccgctaat
     1441 tgcctaacaa tttcatcatc cgatcgacga cgtcgggtgg ctgtgagacc acttctttcg
     1501 ccagtttcgc caactcctca ccgccgatcg catcgaccgc ccagcggcgc ttcttcaaat
     1561 cttcgagcag cgccggatct ttcagcgagc gttcgtacgc gtcgcgcaaa attttaacgc
     1621 ggtcgggtgg cacgcccggc gtagcgacta acggccggcc gaagaccgcg ggagcgagca
     1681 gaaccgtagc tacccgtcgg atcgcctccg tagttttata ttgatccatc aactcataga
     1741 tagacggcac atctggaacc agcggatcgc gcttttttcc cgtctgcaga atcggccgga
     1801 caaacccttt cttgtgccag gtaagaaacg gttcgcgggc gaaataagtg ctgattgtca
     1861 gattgcggca gtgcaactcg ccgcgctcaa tacctaaatc aatctcaccg ccgccgggat
     1921 agccgctgac gatgttgaac ttgatcccga gcccttcgtc gagaaatttt ggcatgtaat
     1981 aaccggaagt gctggtgccc gtggcgccgc atttcggcgg ctgcgcggcc ttgcgcacgt
     2041 cctcgatcga ttggtacggc gtgtcggtcc tcatgactag aataaattcg gtttgttccg
     2101 gcgtaccgat ccaggtgaat ttcgcccagt caaagcgaac ttccggacgc ctggccagct
     2161 ggtcgaagta catcgatggg ccgaatgtgc cgagggtgag accatccggc ttcgccaccg
     2221 aataaacata gttcgcggca accactccgc ctgcgccagt catattttgc acaatgaagg
     2281 tgggatttcc ggcgatatat ttgcccatgt actgtgcgaa gattcgtgcc caaagatcgt
     2341 acgcgtcgcc cgggagattg ccgacgatga tcctgatcgt ttttccctgg tagaaaggcg
     2401 cctgagcatt cacctcggcg atccacgaca gcacaaacaa aagcgcgcac gcgcacacca
     2461 accgcttcat aacacctcca cactgtttgg aagcaagcct cgaccgattg tcagatacga
     2521 aatcaccggc gagcttaggg cgatgagaac aatcgtgtca aggtgcgaag atagccaaat
     2581 aagagcgcca gtcacagtgc aggcctgtat ccagtatttt catactaccg cgttttgtat
     2641 tgacgctatt tgcgtcggtg catataacgg gctctgtcag tttgacgaag ggtcacgttg
     2701 ttgttatcgc aacgggcggt tccagtcgtt taaacgaggt tactcatgca tggcatcgcg
     2761 aagcattgga tcatctatgc gagggttttt ttcacgctca cgctggcgat agtctcggcc
     2821 gcccaggctc aagacaagct gctgatctcc tacggtggtc acaatgaaac ggttggcccc
     2881 atgtgggtgg ccgcggatag gggcattttc aagcgataca atctcgacgt gaatatgctc
     2941 caggtacgta atggccagat cagcctaacg gcggtcatgg caggggatgt ccaggcgttt
     3001 tggccagccg tttcttccgt tataagcggc gtcggcgccg gagcaaagct cggctgcgtc
     3061 gctagcccgt tcaacaaaat cgccagagag ttgatggtgc ggccagaaat tgactcactt
     3121 gccgcgctgc gggagaaaat cttcggcgtc cagagcattg gcggcggcct ctggttgcaa
     3181 acgatgatcg ttttggatcg gctcggcgtc gatgccgaca aatacaaact gcagatgcgc
     3241 gtgatcggcg acgaaccaac gattcttcaa gccctgctcg cctcgaatgt cgacgcagcg
     3301 gtaatcaccc aggcttcggc cgcggtggcc aaacgggcag gtctgcgctc gctggcaaat
     3361 accatggagc tgaaggtgcc ttatcaaggt gttggaatct gcacacgtag cgaccggata
     3421 gttaattcgc ccgacctcat catgcggttg accaaaggta tggtcgaggg cgtggtatat
     3481 attcatagcc cgcgcaacaa acgcgacgtc atggaggtgc tcaagaagca tctccgccta
     3541 gccacagacc aggacgccga aacctcttat aacgccctgc gcacagtgtc ttccctcgat
     3601 ctcgccccgg atccagaagc ttggaaaaat attcaaaagt atgtcgcgcg ggtgacacct
     3661 aaagtcgccc agttggatat ccagcaaatc ataaacggta atttcgtcaa aacgctcgaa
     3721 gaaaccggct tcatagccga gacgaaaagg cgctacggac tctagccata ggccagcgaa
     3781 agacgcgcac gttggcattc caacactcca tttgattgtg gtgctcgaat ggtaggtcag
     3841 cggatgaacc tcgagttctg gcccctgcaa ctcacgcaat gacggaagat ctgcaagcca
     3901 acctgcgcgt caccggcgat ccgcgcttgg ccgaggaatg ggagcttgtg ctgttaactc
     3961 agggattgtc accgagcctg cgacgaacgc cggcgggtat gattctcagc gtgcccgaga
     4021 gcgagcgagc acaggcgttt gccagtctcg cggcgtacga tcgggagaac cgaaagccgg
     4081 cgacccatgt cgagcggctg gactcgggaa acttgattgc cggaaccgcg gtcggcctaa
     4141 tgctcttgtt attttattca atcacggtgc agtggtcgcc tggattgtct tggctcgcgc
     4201 gcggcagcgc ggacgctgag cggatacttc agggcgagtt atggcgcgcg gtcactgctc
     4261 tcacgctcca tgccgatatt gcccacgtcc tgagcaatgc tgtcgccatt gcgttatttt
     4321 ttagtgcgct atcgagtatc gtcggtggcg gagttgctgg cgcactggtg gttttcgccg
     4381 gcgcgggggg caatctcgcg actgccttgc tgcatggaag ctccttcgtc tcggtcggtg
     4441 catccacggc ggttttcgcc gcggtcggtt tgctcggcag cctcggcgta acccggcgac
     4501 ggcggagcac tttgagctgg cgacgcgcct ggataccgct agccgcagcg ctcgcacttc
     4561 tcggcatgct cggcagcagc ggccagcggg tcgacatctg gggccatctt tttggtcttc
     4621 tggttggcgc tggacttgga ctaccggtgg gtttgaccgc gccacgagta ttgggattac
     4681 tcagtcaatg ggcgtgtggc acagcagcag tcgtcacagt gatctactgc tggatgctgg
     4741 cgtttcgttg aatcgagtgt caaacgtgcg gtcgccgtag agtcgttcga gggtggcgaa
     4801 tcggtggaag actgcttttt gggtcgtcgg ctttattctt cgggtcaaga tttctgttga
     4861 ggtcctcgat cccgatatct gctgcgagca gtgaatgacc ggcgctacgt catcgcaaag
     4921 tacgctgaac gctttttcag ccgcaatgca ttattgggcc ttggacaaag tttcgagggc
     4981 atttcgcagg gaactggccc agctctggag cgtggtcgtg ctgattctgc cgtccggcgt
     5041 gttaatgccg actccgtcgg tactgatatc caacaggata gcctctaagt cggcaactac
     5101 tttgtgatac ttggtctcgg acaagacgcg gtagttttcc ggcaccgact tatatggctt
     5161 cttcgcaggt gcagtctgag tgtacggcga ggtgattgcg gccggagtcg taatcgagac
     5221 tgacgtgttc aaggtgattc tccctttctc ttgatactgg ctcataatga cccgccgagg
     5281 aaccgagcaa gtagcaggcc aattgcaatc acttgccaca agcggacttt tcatacgaaa
     5341 ttccccaact cgaaggtgcg ccaatcgtcc ggcgttctat atcttagaaa aatcgggctt
     5401 cgttggttct cattaatgaa aagatgcgat aacttcgcca aacgtcgccg aacgactata
     5461 gctaaaaaac cttacctcca ttgcgcatag atgacggcag agttagaatc ggcggcatta
     5521 atatccataa attctgattc gggcggaggg tcttcttgag tctttcattg aagcaaatgg
     5581 ccattggcct tggcgttgtg ctcgtgtgtt tcacaatttt ctttactgtc aacaaacagt
     5641 atcgtcctga cacagatatt atcttcagcg aatttatcgc cgcggtggaa cgcggcgatg
     5701 tcgtcgaagt gatgatcgag ggacatcaca tcgaggggca gttaaaaaat ggcgaacagt
     5761 tcaagacgta tacgcccgat gatccgcagc tggtgaagtc actgcgcgag aaaaacgtga
     5821 aagtcgcggc taaagccgaa ggcgcgtcac cgtggtacgt gacgctgttg gtttcctggt
     5881 tgcccatcct gttgctcata ggagtgtgga tattttttat gcgccagatg aaagtgggcg
     5941 gcggcaaggc catgtctttt ggcaagagcc gggcaagact gctgaccgag aaccagcatc
     6001 gtattacttt tagcgatgtc gcaggcgtcg acgaagccaa agacgacctg caagagatca
     6061 tcgagttttt aaaagaccct aaaaagttca ccaaactggg tggcagaatt cccaaaggct
     6121 gtttgctcgt cggcgccccg ggcacaggca agacgctgct ggcgcgggcg atcgccggcg
     6181 aagccggagt gccgttcttc agcatcagcg gttccgattt cgtcgaaatg ttcgtcggtg
     6241 tcggcgcatc gcgggttcgc gatcttttcg ttcagggcaa gaagaaagcg ccgtgcatta
     6301 tatttatcga cgaaatcgac gcagtcgggc gccatcgcgg cgctggtttt ggtggcggca
     6361 acgacgaacg cgaacagaca ttgaaccagc tgctggtgga aatggatggc ttcgaatcaa
     6421 acgatggggt cattctcatt gccgcgacca accgtcctga cgtgttggat ccagcgttgc
     6481 tccgcccagg gcgattcgat cgccatgtcg tcgtaccgcg accggacatt gccgggcgag
     6541 tcggtatctt gcaggtacac tcgcgcaagg ttccgttggc agcggatgtc gatgtcaatc
     6601 ttctggcgcg atcgactccc ggtcttaccg gcgccgattt ggagaatctc gtcaatgaag
     6661 ccgctttgct agcggcccgc aatgaccagg aaaaagtcaa catgctcgat ctggagctcg
     6721 ccaaggacaa agttatgatg ggcgccgagc ggcgcagcat gattatcagc gacgaagaaa
     6781 aacgcacaac ggcttatcat gaagccggcc atgcgttggt cgcaaagctg ctgccgggtg
     6841 ccgatccggt acacaaagtg acgataatcc ctcggggtat ggctttgggt ctgacccagc
     6901 aattacccga ggacgaaaaa tatacttatg cgtcgaaata cctgctcaat aacctggtta
     6961 ttcttttcgg cggtcgggtt gccgaagagt tggtattcga gcagatcacc acaggggcag
     7021 gcaacgacat tgaaaaggcc accgatctgg cgcgccggat ggtctgcgag tggggcatga
     7081 gcgagaagct aggaccgatg accttcggc
//
LOCUS       DATLCH010000025         6027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_828587, whole genome shotgun sequence.
ACCESSION   DATLCH010000025 DATLCH010000000
VERSION     DATLCH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828587"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VMZ02_00690"
     CDS             <1..1415
                     /locus_tag="VMZ02_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUR70468.1"
                     /translation="GLFAGVAPGALEFFNHFFWWGALGSILLLIPYFPRSKHIHIFLA
                     PINLALKKQNPGALQPMDFEKEETFGAAKLEDFTWPRLLDSYSCIMCNRCQDVCPATN
                     TGKALSPAAILINERYELNKIFPSFSQGQESPRPLLDFALDAESAWACTTCNACIEVC
                     PVGNEQMLHIIDVRRERVLGAAEFPKGLQNTFNHMERAGNPFGLAADERVAWTKGLPF
                     AVPTLADKPDPDVLYWVGCAVAFDPRAQKIAQSMATIFNSAGVDWAVLGKDEKCTGDT
                     ARRAGNEYLFVQMAEENIGALNQIKAKTIVTTCPHCFHTIANEYPQFGGNYVVKHHTD
                     FIAELSAAGKLKSNLASNGSVTYHDPCYLGRHNGIFDQPRVLIESTGAKLTEPSRSRS
                     NSFCCGAGGGQFWKEEEKGRERVSTNRFRELKQTGAKTVATGCPFCMRMLTDENAKEE
                     PEGAMEVVDVAEIVARSLEK"
     gene            1417..1878
                     /locus_tag="VMZ02_00695"
     CDS             1417..1878
                     /locus_tag="VMZ02_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HUR70469.1"
                     /translation="MRTSGGGVTVFFWLVLFIVSACDAQPKVTINTKDGRQVSFQVEV
                     ADTPGKREIGLMYRTELADDHGMIFLFPSESQQSFWMKNTPRALDMIFIGSDRKIVGI
                     VEQATPFSLEPRAVEGRSQFVFEINGGLSKRHGFKTGDSVQFDGFNASVSQ"
     gene            complement(1933..3000)
                     /locus_tag="VMZ02_00700"
     CDS             complement(1933..3000)
                     /locus_tag="VMZ02_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70470.1"
                     /translation="MAQKKIVHIVGTGTIGEPLIGILSTFKEQFGIDEVTFHKRTPLL
                     TDRSKVLVSCDKGAKLAVDKENRQKFIEMGMTPCLEAEEAIERASVVIDCTPVGNKNK
                     EKLYNKYANNGRGFIAQGSEDGFGKKYARGINDKSLVTGEDQFIQVVSCNTHNLAVLI
                     DTIALGPEKEDNLAESRFVCMRRANDLSQEGDFIPAPEAGKHDDKKYGTHQGHDAHEL
                     FKTMGLDLNIFSSALKLNTQYMHTIHFNLRLRRSIEMDELLRRFRSTRRVAMTNKASS
                     ASVFSFGRDHGLFGRILNQTVIVAQSLAIHNDNEVIGTCFTPQDGNSLLSSLAASLWF
                     MYPDSYEEKLAPVRPYFFSEI"
     gene            3135..4583
                     /gene="tldD"
                     /locus_tag="VMZ02_00705"
     CDS             3135..4583
                     /gene="tldD"
                     /locus_tag="VMZ02_00705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HUR70471.1"
                     /translation="MSDTLVPAQSFFTKNFGIGNQDLEKIIDAAVGKKADYADLFFEY
                     RRNEGISLEEGMVKNCSQSTSNGVGVRVLAEAKTGYAYTDDITIENLEIAARTARYIA
                     QSRPSQVPAQVGQRPGAAHDLYSIATPVNDVALDRKVALLYEIDKFTRALDPRVKNVF
                     ASIGAEYKVILVASSQGMVVGDIQPLTRLNITCIVEENGNRQIGSFGGGGRVEFGFFV
                     DARDHERYAREAVRQAVLNLSAEDAPAGTMDVVLGPGWPGILLHEAIGHGLEGDFNRK
                     KTSAFSDRIGEKVASELCTVVDDGTIPSRRGSINVDDEGTPTSRTTLIEKGILKGYLQ
                     DKLNAGLMKMSLTGNGRRESYAHIPMPRMTNTFMLAGESAPEDILKSVDKGLYAVSFG
                     GGQVDITSGKFVFSASEAYLIEDGKVTKPVKGATLIGNGPDVLTRVSMVGSDLKLDEG
                     VGTCGKDGQSVPVGVGMPTIKIDGLTVGGTRA"
     gene            4677..>6027
                     /locus_tag="VMZ02_00710"
     CDS             4677..>6027
                     /locus_tag="VMZ02_00710"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HUR70472.1"
                     /translation="MERCELTQELGREILAAATKKGASSGDVVMVEGDSFFVTVRMGE
                     VEKISQAGEKRLGLRLFLGNSSASASTSDISKKSIEKLVDDTVQMARATAQDPSGGLP
                     EADMLAREIPDLDLVDNGARNLAVDDKIQIALDTEKAALAFDARITNSEGAEFSNHFG
                     RVVYVSSHGFAGEYSGSTFGHSVSPVAKQDGSMQRDYWYSSKRKFADLESSKHVGETA
                     AQRVLRRLGGRKVKTCEVPVVFDPEMAASLLRSLASAISGYALYKGASFLAGKLGEKI
                     GSDLLTVIDDGTIVGAHGSRPFDAEGLPIRKKIVVERGELKSYLLDTYSGKKLAMPST
                     GNASRSVGEPPGVSPANFYLNRGAQTPQQIIGSIKSGLYVTEMIGSGVNMITGDYSRG
                     AAGMWIENGELAYPVEEITVAGNLKEMFHDIEMVGNDLEMRGRIAAPTLKLVKMTVAG
                     "
BASE COUNT         1329 a         1607 c         1742 g         1349 t
ORIGIN      
        1 ccggactttt tgccggtgtt gctccaggtg ccctggagtt ttttaatcat ttcttttggt
       61 ggggcgctct cgggtcgatt ttacttttaa ttccctactt tccgcgctcc aagcacattc
      121 atatattcct cgcgccaatc aatttggctt tgaaaaaaca aaatcccggc gcgctacaac
      181 cgatggactt cgagaaagag gaaacctttg gcgcggctaa actggaggac ttcacctggc
      241 cgcgtttgtt agactcctac agctgcatca tgtgcaatcg ctgccaggat gtttgtccgg
      301 ctacgaacac cggcaaggcg ttgagcccag ccgcgattct catcaatgag cgctatgagc
      361 tcaacaagat tttcccgagt ttctcgcaag gtcaggagag cccgcggccg ttgctcgatt
      421 tcgcactgga tgcagaatcg gcgtgggcgt gcacgacctg taacgcgtgc atcgaagtgt
      481 gcccggtcgg caatgagcaa atgctgcaca ttatcgacgt gcgccgggag cgcgttttgg
      541 gcgccgcaga gtttcccaag ggactgcaaa acactttcaa tcacatggag cgcgctggca
      601 atcctttcgg gttggctgcg gacgaacgag ttgcctggac caagggactg ccattcgcgg
      661 tgccgacatt ggccgacaag cctgatccgg atgtgctcta ctgggtagga tgcgccgtgg
      721 ctttcgatcc gagggcgcaa aagattgctc agagtatggc gaccattttt aacagcgcgg
      781 gcgtggactg ggcggtactc gggaaggatg aaaaatgtac cggcgacacg gcgcggcgcg
      841 ccggcaacga atatttattc gtccaaatgg ccgaagaaaa catcggcgcg ctgaaccaga
      901 tcaaagccaa aaccattgtt acgacttgtc cgcactgctt tcacacgatt gccaatgaat
      961 acccgcagtt cggcggcaac tatgtagtta agcatcacac ggatttcatc gccgagctga
     1021 gcgcagccgg caagctcaaa agcaatctcg cgagtaacgg ttcggtgacc taccacgacc
     1081 cctgttacct cggccggcac aacggcattt tcgatcagcc gcgcgttctg atcgagtcca
     1141 cgggcgccaa attgaccgag ccgagccgga gccgtagcaa cagcttctgc tgcggcgccg
     1201 gcggcggtca attctggaaa gaagaagaaa aaggccggga gagggtcagt accaaccgct
     1261 ttcgtgaatt gaaacagacc ggcgccaaaa ccgttgctac gggctgcccg ttttgcatgc
     1321 gcatgctcac ggatgagaat gccaaggaag aaccggaggg cgcaatggag gttgtcgacg
     1381 tggctgagat tgtcgcccgc agtttagaaa aataagatgc gaactagcgg cggtggggtg
     1441 acggtttttt tctggctcgt gttgtttatc gtgtccgctt gcgatgctca acctaaggtg
     1501 acgattaaca cgaaagatgg ccgccaggtc agctttcaag tagaagtcgc cgacactcca
     1561 ggcaagcgcg agatcggctt aatgtaccgt accgaacttg ccgacgacca cggcatgatc
     1621 tttctttttc cgagcgagtc ccagcagtcc ttctggatga aaaatacccc gcgcgctttg
     1681 gacatgatct ttatcggtag cgatcgtaag atcgtcggca tcgtcgaaca agccacgccg
     1741 ttttccctcg agcctcgggc agtcgaaggt cggagccagt tcgtttttga gatcaatggc
     1801 ggcttgtcca aacggcacgg cttcaaaacc ggcgactcgg tgcagttcga cggttttaac
     1861 gcctcggtca gtcaataaaa aaggaccgaa agccggagcc gtcggtccct tttatctgct
     1921 taaaaactgt tgtcaaattt cgctaaaaaa gtacggccgc accggcgcga gtttctcctc
     1981 gtaggaatcc ggatacatga accacaacga ggcagcgagg ctgctcagga gcgagttgcc
     2041 gtcctgcggt gtaaagcagg tgccgatgac ttcgttgtcg ttatggatcg ccagactttg
     2101 cgcgacgatc actgtctgat tgagaattcg accgaacagg ccatggtcgc gtccaaacga
     2161 aaacaccgac gccgacgatg ccttattggt catcgcgact ctgcgcgtcg agcggaaacg
     2221 gcgcagtaat tcgtccatct cgatgctgcg gcgcaagcgc aagttaaaat ggatcgtatg
     2281 catatactga gtattgagct tcaacgccga ggagaagata ttcaaatcaa gtcccatggt
     2341 tttaaataac tcgtgggcgt catgaccctg atgggtgccg tacttcttgt cgtcatgttt
     2401 gcccgcctcc ggcgctggaa tgaaatcgcc ttcctgactt aaatcgttgg cgcggcgcat
     2461 gcagacaaag cggctctcgg ccaggttgtc ctctttttct ggacctagtg cgatcgtgtc
     2521 gatcagcacg gctaagttgt gagtattaca actgaccact tggatgaatt ggtcttcgcc
     2581 cgttaccaga gacttgtcgt tgataccgcg ggcgtatttt ttgccgaagc catcttcgct
     2641 gccctgcgcg ataaagccgc ggccgttgtt ggcgtattta ttgtacaact tttctttatt
     2701 tttattgccg accggcgtgc aatcgatgac gacgctcgcc cgttcgatcg cttcttctgc
     2761 ttcgagacat ggcgtcatgc ccatttcgat aaacttctgc cggttttctt tgtccacagc
     2821 gagtttggca cccttgtcgc acgagactaa gactttgctg cgatcggtaa gcagcggcgt
     2881 gcgtttgtgg aacgtcactt cgtcgatgcc aaactgctct ttgaaggtag acagaattcc
     2941 gatcagcggt tcgccgatgg tgccggtgcc gacaatgtga acgatctttt tctgtgccat
     3001 ggttttcagt ccctcggttt gttggccaaa tactagccca agcaagtcat gcgatcaaca
     3061 gattatccgc caccgagcac tagtttttgg ctttaaatcc tctatcttag aatgcataag
     3121 cgaggtgaat cacaatgtca gacacgctgg ttccagctca atcgtttttt actaaaaact
     3181 tcggcattgg caatcaagat ttggaaaaga tcatcgacgc ggcggtgggc aaaaaagccg
     3241 actacgccga tctatttttc gagtaccgcc gcaacgaagg catcagcctg gaagagggga
     3301 tggtcaaaaa ctgttcccaa tcgacttcca atggcgttgg cgtgcgtgtg cttgctgagg
     3361 cgaagacggg ttacgcttac acggacgaca tcaccatcga aaacttagag atcgctgctc
     3421 gtacggcgcg ctacatcgcc caaagccgcc cctcgcaagt gcccgcccaa gtgggccagc
     3481 ggccaggcgc cgcccatgat ctttatagca ttgcgacgcc ggtgaacgac gttgcattgg
     3541 atcggaaagt cgcgttgctt tatgagatcg ataagtttac ccgcgcccta gaccctcggg
     3601 taaagaacgt tttcgcgtcg ataggggccg aatacaaagt aatcctcgtc gcgtcgtcgc
     3661 aaggcatggt ggtgggtgat atccagccgc ttacgcgcct caatattact tgcatcgtcg
     3721 aagaaaatgg caaccgccag atcggcagtt tcggcggcgg cgggcgggtg gagttcggct
     3781 tctttgtcga tgcccgcgat catgaacggt atgcccgcga agcggtgcgc caagccgtgt
     3841 taaacctgag cgctgaagac gcgccggcgg gaaccatgga tgtggttctc ggtcctggtt
     3901 ggccgggaat tttgctgcac gaggccatcg gtcatggtct tgaaggcgat ttcaatcgca
     3961 aaaaaacttc cgctttcagc gaccgtatcg gcgaaaaggt cgcgtcggag ctttgcacag
     4021 tggtggacga cggaaccatc ccgtcgcgcc gcggttcgat taacgtcgac gacgaaggca
     4081 ctccgacgag ccgaaccacg ctgatcgaga agggcatact caaaggctat ctgcaagata
     4141 agctcaatgc cggtttgatg aagatgtcgc ttaccggcaa cggccggcgc gaaagctacg
     4201 cccatattcc catgccgcgt atgaccaaca cgttcatgct ggctggcgag tcggcgccgg
     4261 aagatatttt gaaatcggtg gacaaaggcc tctacgctgt ctccttcggc ggcggtcaag
     4321 tcgacattac cagcggcaaa tttgttttct ccgccagcga agcttacctg atcgaagatg
     4381 gcaaggtcac aaagccggtg aagggcgcga ctttgatcgg caacggccct gacgtgttga
     4441 cgcgagtgtc gatggtcggc agcgatttga agctcgatga aggcgtcggc acctgcggta
     4501 aagacggcca gtcggtgccg gttggcgtcg gtatgccgac gatcaagatc gatggactga
     4561 cggtgggcgg gacgcgggcg tagtcgaaaa gtctgttgtc aaaggtttca atggttccat
     4621 ggctcgattg aaacagttgg aaccttggaa cagtcaaaac gatagatcgg aaaaccatgg
     4681 aacgctgtga attaacccaa gagctcggtc gcgagatact cgccgccgcc acgaagaagg
     4741 gtgcttcgtc cggcgatgtg gtcatggtcg agggcgattc gtttttcgtt acagtgcgca
     4801 tgggtgaagt cgagaagatc agccaggccg gtgagaagcg gctggggctg cgcctatttc
     4861 tcggcaacag ctcagcgtcg gcgtcgacgt ctgatatttc caagaaatca attgaaaagc
     4921 tcgtcgacga cactgtgcag atggcgcgtg ccactgctca ggatcccagc ggcggcctgc
     4981 ccgaggcgga tatgctcgcc cgggagattc ccgatcttga tctagtcgat aacggcgcgc
     5041 gcaatcttgc cgtcgatgat aagattcaga tcgccttaga caccgaaaag gctgcgctgg
     5101 ccttcgatgc gcgtatcact aactccgaag gcgccgaatt ttccaaccac ttcggtcgcg
     5161 tcgtctatgt cagcagtcac ggctttgccg gggaatattc cggctcgacc ttcggccatt
     5221 cagtgtcgcc cgtggcgaag caggacggtt cgatgcagcg cgactattgg tactcttcga
     5281 agcgaaagtt tgccgatttg gagtcgtcca agcatgtcgg cgaaacagcc gctcagcgcg
     5341 tgctgcgccg gctcggcggc cgcaaagtga aaacctgcga ggtgccggtg gtgttcgatc
     5401 cggaaatggc agcctcgctg ctgcgcagtc tggcctcggc aatttcggga tatgcgctct
     5461 ataaaggcgc gtcgttcctg gccggcaagc tcggcgaaaa gatcggctcg gatttactaa
     5521 ccgtgatcga tgatggcacc atcgtcggtg ctcacggctc gcgccctttc gatgccgagg
     5581 ggttgccgat taggaagaaa attgttgtcg agcgcggcga gctcaagagt tatctgctcg
     5641 acacttatag cggcaagaag ttggcgatgc cgtctaccgg caacgcctcg cgttccgtgg
     5701 gcgagcctcc gggtgtgtcg ccggcgaatt tttacttaaa ccgcggcgca cagactccgc
     5761 agcagatcat cggatcgatt aagtcggggc tctatgtcac cgagatgatc ggttctggtg
     5821 tcaacatgat caccggcgat tactcccgcg gcgccgccgg catgtggatc gagaacggcg
     5881 agctcgccta tccggtagag gagatcaccg tcgccggcaa tctcaaggaa atgtttcacg
     5941 acatcgaaat ggtcggcaac gatctcgaaa tgcgcggccg catcgcggcg ccgacgttaa
     6001 agcttgtgaa gatgacggtg gcggggg
//
LOCUS       DATLCH010000026         5913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10477348, whole genome shotgun sequence.
ACCESSION   DATLCH010000026 DATLCH010000000
VERSION     DATLCH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10477348"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            458..844
                     /locus_tag="VMZ02_00715"
     CDS             458..844
                     /locus_tag="VMZ02_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70473.1"
                     /translation="MDFQIKLFNRQDEGNHLVFLTRGSMDAAAFRQLFAEIEKAALGL
                     QECKVLVDLSDSTYQIDPTEIEELAAATQLTRWPRTNLVAIVSTPKIADYRRLYFLRT
                     ELAARGLAVKIFRYSRIAIDWLAVPG"
     gene            complement(1107..2225)
                     /gene="ydiK"
                     /locus_tag="VMZ02_00720"
     CDS             complement(1107..2225)
                     /gene="ydiK"
                     /locus_tag="VMZ02_00720"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HUR70474.1"
                     /translation="MNNSPQDLTHITLRLLFMGLLIGGSFWILKPFLLALIWAIMIVV
                     ATWPLLLKAQSMLGGYRSLAVTVMIAAALIILVAPITVAVVTVVDHSLDIAAGSKWLM
                     AFVKAPPPDWVKSLPLVGEKIVTEWRRIADDGAAELAVRGLPYVDNVLGWFVSQLGGL
                     GLMLFHFALTLLISGILYYHGEKAADAVRRFAARLGGAQGENAIHLAGQAIRGVALGI
                     VVTAVAQSALAGIGLALVGIPYAAILSAVMLLLGIAQIGPMPVLVPAVIYLYWAGNPG
                     LGTVLLVWTAVVGTMDNFLRPILIRRGADLPLLLIFAGVIGGLFAFGVIGLFVGPVVL
                     AVAYTLLNAWVDGPAPIATVPVVATASQVREESIRASD"
     gene            complement(2344..3063)
                     /gene="pgl"
                     /locus_tag="VMZ02_00725"
     CDS             complement(2344..3063)
                     /gene="pgl"
                     /locus_tag="VMZ02_00725"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HUR70475.1"
                     /translation="MKIEVFSDPAAVANKAAEIIAAQACAAIAARGRFVVGFSGGRTP
                     SAMLRALAEQDLPWRNVHVVQVDERVAPAGHADRNLTQLHESLLALAPLPTEQIYAMP
                     VEAADLAAAAESYAGTLRQIAGVPPVLDVAHLGLGPDGHTASLVPGDPVLEVDDKDVA
                     VTGVYQGRRRMTLTYPMLNRCRRILWLVTGADKGDALVSLIAGDASVPAGRIQRDNAV
                     ILADRAAAEKSAANELQPTAQ"
     gene            3939..5114
                     /locus_tag="VMZ02_00730"
     CDS             3939..5114
                     /locus_tag="VMZ02_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HUR70476.1"
                     /translation="MRRVLIYSFLLVAGMVLSQLPIVQSSNAAIMPLTMIFLAYIMIE
                     VGMEFDIDKSRLSSYAVDYAIAMAAAAVPWLCAAFYFWWFFSLGFKESLLVGRFSSPT
                     SAGILFTMLAAAGLASTWVYKKARVLAIFDDLDTVLLMIPLKMMLIGFEPKLLILILA
                     VFLFLAAAYFCIHWLAVPTGNAWIMIYAVLVWGVTYAFDYVTELHLEVLVPAFALGCV
                     IKSDHLHGSAEYAGTDFRTSAPDWRAQAILDWTIKGGFMVLAGTALPPIQLGGMPGGI
                     VLAHVILITCASNIGKCVPMLAYRDEVSLRERLALSIGMFPRGEVGIGVLLVSLDIFR
                     QQNMLDSPAIRQSMTIAGLSLALNLALTGVFILAMIRLMGTPRLRPISGKSSERDHR"
     gene            complement(5105..5236)
                     /locus_tag="VMZ02_00735"
     CDS             complement(5105..5236)
                     /locus_tag="VMZ02_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70477.1"
                     /translation="MLAFTSINIETHVEDLEPRTYANTPLDAADAADAADAAAPTQR"
     gene            complement(5338..>5913)
                     /locus_tag="VMZ02_00740"
     CDS             complement(5338..>5913)
                     /locus_tag="VMZ02_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70478.1"
                     /translation="KISVILLSVILAGGLTSMAAAQSKEEAVKEGKPGGVVVDVLKLS
                     GTVKAIDMDKKMVTIEGSGGRSVVVDAKNARNLDQVKVGDKVNLQYIESMALFVRKTD
                     APPSAIEAQEVQLAPKGEKPGGIVAKTIELTGNVESIDTQKRTIALKGPAGNVRTFKV
                     GTEAKNFGQIKKGDQVVLRFTEAMALSVVKP"
BASE COUNT         1282 a         1700 c         1617 g         1314 t
ORIGIN      
        1 gcagttttac gccttcgatg tactcctaca ccgcggcgca gcgttctccg gttgccaatc
       61 gaggatcggc gccagttact ctccgatgca ttgcgaaacg acaagtatcg ggtgtatgtc
      121 aacaccattc gatgttaaag cccgcgaaac cgacgcgtgc ggctaaggag ttcgagttcg
      181 aggctgttgt tggcaagcgt gaaggattcc ctgtatggaa ccgggcgagc gcagcgcgct
      241 tgggcgaaat ccaaaataaa ccgcttcagg aattgtgatt ggcggttacc ccgtaggaca
      301 ccaggatgcg cttatcgtcg gcttaggtga ggcaaggcaa cattttgcta tcaacactcg
      361 accccaagca tgggaaatcc cgccgactgt cgatcaagtg tcgccaaatg tcaagaaaat
      421 gcgccgccga gctcttaacg tgacggcgga ggcgctgatg gactttcaga tcaagttgtt
      481 caatcggcaa gatgaaggaa atcacctggt tttcttaaca cgcgggagca tggacgccgc
      541 ggcttttcga cagctcttcg ccgagatcga aaaggcggcg ctcgggttgc aggaatgcaa
      601 ggttctcgtg gatttgtccg atagcacgta ccagattgac ccgaccgaaa tcgaagagct
      661 tgccgccgca actcagctga ctcgttggcc tcgaaccaac cttgttgcga tcgtctcgac
      721 gccaaaaatt gccgattatc gccgtttgta tttcctgcgt acggagctgg ctgctcgggg
      781 tttggccgtc aagatttttc ggtattcgag aatcgcaatc gattggctcg ccgtgccggg
      841 ttaaaggtcg cgccgaccaa cccggggcta gacacggcct aacaattcgc ttgagtagga
      901 aaatggacgc caactcacaa cagaagaatc tcaatggcaa aaagctcggg tcaaatgcct
      961 cagtcgagaa aacccaagtt acgcggcaaa gttggtttcg attttgttcg gcggcgacca
     1021 ccgccaggtg atcagcgctc gacacctttc aaagcatgaa attgatcgtg aaacgtacat
     1081 ggaagagcta gatgcggcga cgcggatcaa tccgacgcgc ggatactttc ctcgcggacc
     1141 tgactggcag tggcgacgac tggaacagtc gcgatcggcg cgggcccgtc aacccatgcg
     1201 ttcaataatg tataagccac cgcaagcacg accggaccga cgaaaagccc gatcacgccg
     1261 aacgcgaata agccgccgat cacgccggca aagatcagca ggagcggcag atcggcgccg
     1321 cgtcgaatca ggatgggccg caaaaaatta tccatcgtgc cgacaacggc cgtccacacc
     1381 aaaagcaccg tgcccaaacc cgggttgccg gcccagtaca aatagatgac cgccggcacc
     1441 agcaccggca tcggtccgat ctgcgcgatg cccaataaca gcatgactgc gctcagaatc
     1501 gccgcatagg gtatgccgac cagtgccagc ccaataccgg caagcgccga ctgggccacc
     1561 gccgtaacta cgatgccgag agccacgccg cggatagctt gccccgcgag atgaatcgcg
     1621 ttctcgcctt gcgcgccgcc aaggcgcgcg gcgaaccggc gcacagcatc ggccgctttt
     1681 tcgccgtgat agtaaagaat cccggaaatc agcagcgtca gtgcgaaatg gaaaagcatc
     1741 aatcccaggc cgccaagctg actgacaaac cagccgagaa cattgtcgac atacggcagc
     1801 ccccggaccg ccagttccgc ggcgccgtca tcggcaatcc tgcgccattc ggtcacgatc
     1861 ttctccccga ccaggggcag actcttgacc cagtcaggcg gcggcgcttt cacgaaagcc
     1921 atcagccact ttgatcccgc agcgatatca agcgagtgat cgacgacggt gactacggca
     1981 accgtgatcg gcgcaacgag aattatcagc gcggcggcga tcatgaccgt taccgcgagt
     2041 gaccggtagc cgcctaacat tgactgcgcc ttaagtagca gcggccaggt cgcgacgacg
     2101 atcatgatgg cccagatcag cgccagcaaa aacggcttga gaatccagaa gctgccgccg
     2161 atgagcaatc ccatgaatag taaacgcaga gtgatgtgag tcagatcttg cggagaatta
     2221 ttcattgagc cctttcatcc gttcgcatta atcgatggtg ccgacttagt ttgtgatcac
     2281 gagtttcgaa cttggaggat tctgccaata tcgaagaaat ggtcctcact gctagtctga
     2341 tgattattgc gcggttggct gcagttcatt ggccgcggat ttttccgcag ccgcgcggtc
     2401 ggcaaggatc acggcgttgt cgcgttgaat tcgcccagca ggaaccgacg catcgccagc
     2461 aatcaagcta acgagcgcat cgcccttatc ggcgccggtc acgagccaga gaatccgtcg
     2521 gcaacgattg agcatcgggt aggtcaaggt catccggcgt cttccctgat agacaccggt
     2581 taccgcgaca tctttatcgt ccacctccaa caccggatct ccgggaacca atgaggccgt
     2641 gtgaccatcg ggtcctaatc ccaagtgcgc gacgtcgagc accggcggca cgccggcaat
     2701 ctgtcggaga gtgcctgcgt agctctcggc tgccgcagcc aaatccgccg cttccaccgg
     2761 catcgcgtag atttgctcgg taggtagcgg cgcgagcgca agcagactct cgtgcaattg
     2821 cgtcaggttt cgatcggcat ggcccgcggg ggccacacgt tcgtcgacct gcacgacatg
     2881 gacgttgcgc cacggaagat cttgctcggc taaggcacgc agcattgccg atggcgtgcg
     2941 gccgccgctg aatccaacaa caaaacggcc gcgcgccgct attgcagcgc aagcctgcgc
     3001 agcaatgatt tcagcagctt tgttggctac cgcagcagga tcgctaaaca cttcgatttt
     3061 catggcagct cccgcacaaa atggaagacc gtttaatggc gcgacagcat cacgcgcggc
     3121 cggcaaaaca ctgcgcccga aaagaagctt aaatacgcgc atgacatggc cgtttcgcag
     3181 ttagtaatta ttgaaccacg cgctccgtga tcgcgcgact cgtcaaatag catactgtta
     3241 ttccgatcgt gtagattcaa gggcagcgga accgctacgt aggattgcca acttgagatt
     3301 cgggctaagc ccaagcgact actcggctga ttcgagccgg ccataggttt taacaatcta
     3361 tttgaaacgc gctttgcggt cgctccggac actttgtggc cgcgcaagag acgctcgttt
     3421 accggtatta aattattcaa cgctcgcggc ctgctgcgtt gatatgtcgc caagcatgcg
     3481 ttagcaattg agccacggta tttcaaatgt cggcccgcac tttatatgac aagcatcatc
     3541 ggacgatcag ccagatagct gcgtcggcta ttctgattgt cgcttgccta tggatttttg
     3601 tcggcagcat cgatgggccg tcggtgcggt ttgggtcgag atctggctct gacattttca
     3661 aatccgccaa gctaacggat gctggccata gcgaacaacc ttggacgctc tgggtcggag
     3721 tattttcaca acctcccctg ccggttcatg catttttcct gttgttcgat ttcaagcaaa
     3781 accttcgtcg ctggcccaat gtagccggcg atatcagtcg ctctccgcct tacgctctcg
     3841 cggcttaacc gtccgtttaa tatccgatag ttgttatcgt ccctctgcgg cgttggtgcg
     3901 tgaatttcag cgcctgagcc tcggcacgga gttattttat gcggagagta ctgatttaca
     3961 gtttcttgtt ggtcgcgggc atggttttgt cccagctgcc gatcgttcaa tcgtccaacg
     4021 cagccatcat gccgctgacc atgatattcc tagcctacat catgatcgag gtgggcatgg
     4081 agttcgatat cgataagtcg cgcttgagct cgtacgcagt tgattacgct atcgccatgg
     4141 ccgcggcagc agtgccatgg ctgtgcgcgg cgttttactt ctggtggttc ttcagtcttg
     4201 gcttcaaaga gtccttattg gtgggacggt ttagctcgcc gacctcggcg ggaattttgt
     4261 ttaccatgct cgctgccgca gggctggcta gtacctgggt ttataaaaag gcacgtgtgc
     4321 tggcgatctt cgacgatctt gacaccgtcc tattgatgat tccgctcaag atgatgctca
     4381 ttggatttga gcccaagctg cttatcctta ttcttgccgt atttctgttt ctcgcggctg
     4441 cttatttctg cattcattgg ttagcggtcc cgacgggcaa cgcatggata atgatctatg
     4501 cggtgttggt ctggggcgtg acatacgctt ttgactatgt gaccgagttg cacctcgaag
     4561 ttttggtgcc ggcatttgcc ctcgggtgcg ttattaagtc ggatcatctt cacggctcgg
     4621 cggaatacgc cggaacggat tttcgaacaa gtgcacccga ctggcgcgcc caggcaatct
     4681 tggactggac gatcaaaggc ggattcatgg tgctcgcggg tacggcgctg ccgccgatcc
     4741 aattgggtgg catgcccggc ggcatcgtgc tggcgcacgt gatcctcatt acgtgcgcca
     4801 gcaacatcgg caagtgcgta ccgatgctgg cgtacagaga cgaggtttca ctgcgtgaac
     4861 gcctggcgct cagcatcggc atgtttccgc gcggcgaggt tggcatcggt gtgctgctgg
     4921 tctcgctaga tatctttcgt cagcagaaca tgcttgattc gcccgcgatc cgacaaagca
     4981 tgaccatcgc cggactcagc ttggcactca acctcgcgct cacaggagtt ttcatcctgg
     5041 ccatgatccg gttgatgggt acgccaaggt tgcgtccaat cagcggcaaa tcgagcgagc
     5101 gagatcaccg ttgagtcggg gcggctgcat cggctgcatc ggctgcatcg gctgcatcga
     5161 gcggcgtatt ggcgtacgtc cgcggttcga gatcctcgac atgggtttca atgtttatcg
     5221 acgtgaacgc caacatggcg cacaaccaga cagaggcgaa tcgccaccag cagcgcacca
     5281 aaaaaacaat ttggacaatc acccgctcgg gagagcaccc ggcgagcggg cgacgcgcta
     5341 tggctttact accgacagag ccatcgcttc ggtaaagcgc aacaccacct gatcgccctt
     5401 tttaatttgc ccaaagtttt ttgcttccgt accgactttg aacgttcgaa cgttgccggc
     5461 agggcctttc aaagcgatcg tacgtttctg ggtatcgatc gattcgacat tgccggttag
     5521 ctctattgtc ttcgctacga tcccgcccgg cttttcaccc ttgggcgcaa gttgtacttc
     5581 ctgcgcttca atcgcgctcg ggggagcatc tgtctttctg acgaaaagcg ccatggactc
     5641 gatgtactgc aaattcactt tgtcgccgac tttgacctga tccaggtttc gagcgttctt
     5701 ggcgtcgacc acaacactcc tgccgcccga accttcgatc gtcaccatct ttttgtccat
     5761 gtcaatcgcc ttcaccgtgc cgcttaactt aaggacatcg acaaccaccc cgcccggctt
     5821 cccttctttg acggcctcct ctttactttg tgccgctgcc atcgaggtca gcccaccggc
     5881 caaaattacc gaaagtaaaa tcaccgaaat ttt
//
LOCUS       DATLCH010000027         5441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10477460, whole genome shotgun sequence.
ACCESSION   DATLCH010000027 DATLCH010000000
VERSION     DATLCH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10477460"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1220
                     /locus_tag="VMZ02_00745"
     CDS             93..1220
                     /locus_tag="VMZ02_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70479.1"
                     /translation="MTQNQRLEALMAAFRKTTYFRWMAKEGVPVVDGYGIEDVREVTT
                     GTWARTGGKAAFLNLYGMEGVTGMYVGEIPAGGALNPEKHFYEEVIVIIEGQGATEVW
                     QDGGNKQLFEWGPWSLFAPPLNTWHRLVNGSRTPVKYLSVSNAPLVMDIVHNEDFIFN
                     CPYQFADRYSAADGYFNQGLKRYESGMQHIWETNFISDIQTASVDAREVKGAGVGITQ
                     FEISGNSLIGHLATWPAARYHKAHFHGAGAILLGLQSSGYVLLWSKELGTRPFEAGRG
                     DEVVEVKWKAGSVYCPGGGWFHQHFNTGAEPARHLALRYGSRIHPIGFKIADKRSEDG
                     VYIDIKEGGTLIEYGDEDPYIRKHYDDELRKTGVRSAMPVL"
     gene            1312..2655
                     /locus_tag="VMZ02_00750"
     CDS             1312..2655
                     /locus_tag="VMZ02_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUR70480.1"
                     /translation="MPEAKLRDKFSASSQFLKNDIWRIHARKLSRRKLSYINPLRILL
                     LAGRRFFEDKCELRASALTFYTLLSIVPVVAMAFGIAKGFGFEKILEAQLLAKFEGQP
                     EVADRILGFARAMLENTKGGAIAGVGVAVLFWSVVKVLGNIENAFNDIWGIPMARPWG
                     RKLADYLSVMMICPILLIVASSLTLLVTTQITTMVEGLAFLGYAGGIIIFLLQLLPYT
                     VIWLLFTFMYIFMPNTKVQLKSAMWGGVLAGTIYQLTQFTYIKFQVGVSGYGAIYGSF
                     AALPLFLIWLQLSWLIVLFGAEVSFAHQNVAVYEFAADCLDLSHRFKRTVALFISHYC
                     VNAFVHAQSAPTAEEIAQQLEIPIRLVRSILFELTQSKILAEIRLRDGELVGYQPGCP
                     IDQLTVTKIVGVLDRHGADTLPMVESSSLEKLREITRKFDEILETAPANLKIQDV"
     gene            complement(2777..3763)
                     /locus_tag="VMZ02_00755"
     CDS             complement(2777..3763)
                     /locus_tag="VMZ02_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70481.1"
                     /translation="MSNYFRAMVLTVGFLVSVPTASQAKITIAYISDSPSSSVPQWIA
                     KETGIFKRFGLDIDLVFIDGSTRGIQALLAGDLGFTEAVGTSAINGKMAGGNIAIING
                     VVNTLPYYIIGNPTIKSRDDLKGRTAAVHIPGTAADFALRLGLKSINLPYNQIKAITV
                     GGGPARIGALINGQAEFTIVSDAEKIKGESVGQKVIIDLAELKVPFLFTCTVTSTKMI
                     REQPETVRRMAEAIAAGVHFFKTNKEETMKIMRKYTRGADRTILEGAYAAYYKLFVED
                     TYPTVEGLRNTLEIQSSWDPKVAKAKAEDFFELRFVDQLRSSGFIDKLYGRR"
     gene            complement(3891..4400)
                     /locus_tag="VMZ02_00760"
     CDS             complement(3891..4400)
                     /locus_tag="VMZ02_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70482.1"
                     /translation="MPNSKFLRAIGDSLQFLGVVNFVVEKAGSSFVVRSAEPVDPAKL
                     VKPNVSERVWETQTSSRRHAKLFREDGALHYEDSYISWLDAQGKRKRRKRFSAQASGT
                     KNLPQLLRTLGRHLDRVEPHAFRVSWGEKEVHIDYQLADGSRGQEILSIAKLRELTVR
                     SRFRRAPRK"
     gene            complement(4419..5237)
                     /locus_tag="VMZ02_00765"
     CDS             complement(4419..5237)
                     /locus_tag="VMZ02_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUR70483.1"
                     /translation="MTETFETLLLTRDGAITTITFNRPEKRNAMSPQLHREMYQVLSE
                     LRYDKQTKVVVLTGAGGNFCAGQDLKQYGLEMESQPEWVKDDIREKVRKWRTELLRTL
                     PQPIIARIAGWCLGGALTVTVGCDVAIAADDAKFGLPEVNFGHFPAGETTPILANYLQ
                     SKHGLYYALSGKMMSANEAERIGLISRVVPSAELDNEVNEFAKCLAEKSARALRAVKQ
                     AWYTSMKASPDVGFLISNFISDRIIREQGGRPGLEQFVEKKLRPVSGTMKLEKD"
BASE COUNT         1165 a         1491 c         1470 g         1315 t
ORIGIN      
        1 taaatttcgc aggaaaagac gatgtgcgtt tcgcttaatt atgctaaaga tcgagttctg
       61 gaaacaattc cgcacttagg ggtcaatttc acatgacaca aaaccagcgc ctggaagctc
      121 tgatggcggc ttttcgtaaa accacttact ttcgctggat ggcaaaggaa ggcgttccgg
      181 tggttgacgg ttacggcatc gaagacgttc gcgaagtgac taccggcact tgggcgcgca
      241 ccggcggcaa ggcggcgttt ctgaatctct acggcatgga gggcgtcacc ggcatgtatg
      301 tcggcgagat tcctgccggc ggcgcattaa acccggagaa acatttctat gaggaagtga
      361 tcgtcatcat cgaaggccag ggcgccaccg aagtttggca ggacggcgga aacaaacaac
      421 tgttcgagtg gggcccctgg agtctttttg cgccgccgct caacacctgg caccgcctcg
      481 tgaacggcag tcgcacgccg gtgaaatacc tgtccgtcag caacgcaccg ttggtcatgg
      541 acatcgttca caacgaagat tttattttca actgcccgta tcaatttgcc gatcgctact
      601 ccgctgcgga cggctatttc aatcagggac tcaaacgcta cgagtcgggc atgcagcaca
      661 tctgggagac taatttcatc agcgatattc agaccgctag cgtcgacgcg cgcgaagtca
      721 aaggcgccgg cgtcggcatc actcagttcg agatatcggg caatagcttg atcggccatc
      781 tggcgacctg gccggcggcg cgttatcaca aggcgcactt tcatggcgcc ggtgccatcc
      841 tgttgggctt gcagtcgtcg ggttatgtcc tgctatggtc gaaggagctg ggcacgcgtc
      901 cttttgaagc cggccgaggc gatgaggtcg tcgaggtcaa gtggaaagcc ggcagcgtct
      961 attgccccgg cggcggctgg ttccatcaac atttcaatac cggcgccgaa ccggcacgtc
     1021 atctagcgct gcgctacggt agccgcatac atcccatcgg tttcaagatc gccgacaaac
     1081 gcagcgaaga tggcgtctac atcgatatca aggaaggcgg cacgctgatc gagtacggcg
     1141 acgaagatcc atacattcgc aagcactatg atgacgaact gcggaagacc ggcgtaagga
     1201 gcgctatgcc ggtcctttaa tttgcccacg gtgcaaccgt tcactcggac ctcacaagtt
     1261 tagactcttg caaatttgaa tagaattaga tctgccgagc tcgagagaac tatgccggaa
     1321 gccaaactgc gcgacaaatt ttccgcctcg agtcagtttc tcaaaaacga catctggcgt
     1381 atccatgcgc gcaagttgag ccgccgcaaa ctgtcttaca tcaatccgct ccggattctg
     1441 ctgctggcgg ggcgccgatt tttcgaggac aagtgtgaac tgcgcgcatc ggctttgacc
     1501 ttttatacct tgctctccat tgtgcctgtg gtcgcgatgg cattcggcat cgccaaagga
     1561 ttcggcttcg agaaaatact cgaggcgcag ctgctggcca aatttgaggg acagccggaa
     1621 gtcgccgatc gcatcctcgg ctttgcccgt gcgatgttgg aaaataccaa aggcggcgcc
     1681 atcgccggcg tcggtgtggc cgtcttgttt tggagcgtcg tcaaagtctt aggcaatatt
     1741 gagaacgctt ttaacgatat ctggggcatc ccaatggcgc gcccctgggg ccgcaagctc
     1801 gccgattatt tatcggtgat gatgatctgt cccatcctct tgattgtcgc cagcagcctg
     1861 acgctgttag tcacgaccca aattaccacg atggtcgaag ggctcgcctt cctcggttat
     1921 gccggcggca taattatttt cctactgcag cttttgccgt acaccgtgat ctggctttta
     1981 ttcaccttta tgtacatttt tatgccgaac acaaaagttc agttgaaatc cgctatgtgg
     2041 ggaggagttc ttgccgggac catttatcaa ctgacgcagt ttacctacat taaattccaa
     2101 gtcggtgtgt ccggctacgg cgcgatttat ggcagcttcg cggcgctgcc gttgttcttg
     2161 atatggctgc aattgagctg gttgatcgtt ctcttcggcg ccgaagtctc cttcgcgcat
     2221 cagaatgtcg ccgtctacga atttgccgcc gattgcctcg atctaagcca tcgtttcaag
     2281 cgcacagtgg ctctgttcat cagccactat tgcgtcaatg cctttgtcca cgcgcagagt
     2341 gctccgaccg cggaagagat cgcccaacag ttggagatcc ccatccggtt agttcgctca
     2401 attcttttcg aactgacaca atcgaagatc ctcgcagaga ttcgtctgcg cgacggtgag
     2461 ttggttggtt atcagccggg ctgcccgatc gatcagttga cggttactaa aatcgtcggc
     2521 gtcctcgacc gccatggcgc cgacaccctc ccgatggtgg aatcgtcaag cctggagaaa
     2581 ttgcgcgaaa tcactcgtaa attcgacgag attttggaaa ccgctccagc gaatctaaag
     2641 attcaagacg tgtaaacgtc cgagacgcgg cgcagatttc cgcgcgatat tccgagacca
     2701 ttatcacgga gcgaccctga agatggccgt cagttgttcg aagccttccg ggtagcgatt
     2761 tttgcaggcc cagagactac ctgcgcccgt aaagcttatc gatgaagccg ctactgcgca
     2821 gctgatcgac gaagcgcaac tcgaagaaat cctcggcttt agccttggcc accttggggt
     2881 cccatgagga ttgaatttcc aaggtgttgc gcagcccttc gacggtcgga taggtgtctt
     2941 cgacgaacag tttgtaatat gccgcatacg cgccttccag gatcgtacga tcggcgccgc
     3001 gggtatattt cctcatgatc ttcatggttt cttctttgtt ggtcttgaag aaatgaactc
     3061 cggcggcgat cgcttcggcc atgcgccgca ccgtttcggg ctgctcgcga atcatcttgg
     3121 tcgaggttac ggtgcaggtg aacagaaacg gcaccttgag ttcggccaga tcaatgatca
     3181 ctttctgccc gacgctttcg cctttgatct tctcggcatc gctgacgatc gtgaattccg
     3241 cctgaccgtt gatcaaggcg ccgatacgcg ccggtccgcc gccgacggtg attgctttga
     3301 tttgattgta tggcaggttg atgctcttga gtccgagccg cagggcgaag tctgctgccg
     3361 tccccggtat atgtacagcc gcggtgcggc ccttcaaatc gtcgcgtgat ttgatcgtcg
     3421 gattgccgat gatgtaataa ggcagcgtat tgaccacgcc gttgataatc gcgatattgc
     3481 cgccggccat cttaccgttg atcgccgaag ttcccaccgc ttctgtaaag ccgagatcgc
     3541 cggccaaaag cgcttgtatg ccgcgtgtac tgccgtcaat gaacaccaaa tcgatatcca
     3601 aaccaaatcg tttgaaaatc ccagtttcct tggcaatcca ctgcggcaca gatgaacttg
     3661 gcgagtcgga aatataggcg atggtaatct ttgcttggga tgccgttggt actgacacca
     3721 ggaatccaac ggtcaacacc attgcgcgaa aatagttcga catagagtgc ctccctcgca
     3781 accaggcgaa tggaacttat ctttcctatg gcattgcgac ttctttgact tggcgaccgt
     3841 tttatttgtg caaaagacgc gaatcaatcg acaaaaattc gacgcgctta ctattttcgc
     3901 ggcgcccggc ggaatcggct gcgcacggtc agttcgcgga gtttggcaat gctgagaatt
     3961 tcttgccctc ggctgccatc cgccagttga taatcgatat gtacttcttt ctcgccccag
     4021 gagacccgaa aggcatgtgg ttcgacgcga tcgagatggc ggccaagcgt gcgcagcaat
     4081 tgcggcaaat tcttggtgcc gctagcctgt gccgaaaacc gcttgcgccg cttgcgcttg
     4141 ccttgggcat cgagccagga aatgtagcta tcttcgtagt gcagcgctcc atcctcgcga
     4201 aacagtttgg cgtgccgccg ggagctggtc tgagtctccc aaactctctc cgaaacattc
     4261 ggtttgacga gcttggcagg atcaaccggt tcggcgctgc gaacgacgaa acttgatccg
     4321 gctttctcta ccacgaaatt gactacgccg agaaactgca gcgaatcgcc gatggccctg
     4381 agaaatttcg aattgggcat atttcggagg agttaccgtc agtccttttc taatttcatc
     4441 gttccggata ccgggcggag ttttttctcg acgaattgct ccaaccccgg tcggccgcct
     4501 tgttcgcgga tgatccggtc ggaaataaaa ttggagatca gaaaaccaac atccggtgag
     4561 gctttcatgc tggtgtacca agcctgtttg acagctctca acgcccgtgc gctcttttcg
     4621 gcaaggcatt tggcgaattc attcacttca ttgtcgagtt cagcagacgg gaccacgcgc
     4681 gatatcaggc cgatgcgctc ggcttcgttg gccgacatca tcttgcccga gagcgcatag
     4741 tagagcccat gcttcgattg taaataattg gccaggatcg gggttgtctc gccggcggga
     4801 aaatgaccga agttgacttc cggcaggccg aacttggcgt cgtcggcggc gatcgcaacg
     4861 tcgcagccca ccgtcacggt aagcgcgccg cccaaacacc agccggcgat gcgggcaatg
     4921 atcggttgag gcaaggtgcg caagagttcg gtacgccact tgcgcacttt ctcgcgaata
     4981 tcgtccttga cccattcagg ttgagattcc atctctaggc cgtattgttt tagatcctgg
     5041 ccggcacaaa aattaccccc ggcgccagtg agaaccacga ccttggtttg cttgtcgtag
     5101 cgcagttcgc tcaggacttg atacatctcg cgatgcagtt gcgggctcat ggcgttgcgt
     5161 ttttccggac gattaaaagt aatcgtggta atggcgccgt ctcgcgtgag caataacgtt
     5221 tcaaacgttt cagtcatcaa gcttctcctc tgcttaagtt gtgctcaatc tgacacagac
     5281 gggcgccatc ggctagggtt cgccagtgcg gatcccgtca tgtcggttac acctcggaag
     5341 tgatttttaa aatagagcgt actcactcca accgcgttca tcggccatcc tcaatgatcg
     5401 agtcgtcgat tcatgccgtt tctctggcga cagtttcttt a
//
LOCUS       DATLCH010000028         5603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6760419, whole genome shotgun sequence.
ACCESSION   DATLCH010000028 DATLCH010000000
VERSION     DATLCH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6760419"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..966
                     /locus_tag="VMZ02_00770"
     CDS             346..966
                     /locus_tag="VMZ02_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HUR70484.1"
                     /translation="MENQPYRAQDFKISGLKGISDRTVEMHLKLYEGYVKNTNMLNEH
                     VAEFIQKKKNAATDPGYSELKRHLGFEYGGMVLHEHYFGNLRPGGGGAPTKSFINATE
                     IGFGGFDAWKADFVGVGSMRGVGWAVLYQDPVTHNLSNHWIELHQQGVPPGFKPILVM
                     DVWEHAFLLDYPPSERSKYIDAFFENVDWQVVEKQLGSASATRPVG"
     gene            1071..1406
                     /locus_tag="VMZ02_00775"
     CDS             1071..1406
                     /locus_tag="VMZ02_00775"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HUR70485.1"
                     /translation="MAKNESTDGRDPARLKKVVRMPRPKAVTMTLEYFAPEANTVAVA
                     GEFNSWNEQAQMMKKTKAGWWKTSLKLPPGQYQYRFVVDGQQWVEDPENPNKQLNEFS
                     AYNSVLEVR"
     gene            1412..3343
                     /gene="glgB"
                     /locus_tag="VMZ02_00780"
     CDS             1412..3343
                     /gene="glgB"
                     /locus_tag="VMZ02_00780"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HUR70486.1"
                     /translation="MDDDIAAADSGFVSEQDLRLFREGRHHRLYEKWGAHFGVDSQGA
                     SGVYFTVWAPNAQRVSLIGDFTGWKEQEGLLRCLGDSGVWQGFVPGLHAGALYKYRVY
                     SRHLGYRADKADPFAFYNEIPPKSASIVWGLEYTWGDEDWLRRRRERSALDKPMSIYE
                     MHIGSWRRRRGENNRSLSYRELAPLLTEHVKRLGFTHVEFLPVMDHPFFGSWGYQVTG
                     YFAASGRYGTPQDLMFLIDTLHQNGIGVILDWVPSHFPTDEHGLGYFDGSHLYEHADA
                     RQGMHREWGSYVFNYGRNEVRSFLISNGLFWLDRYHADGLRVDAVASMLYLDYGRSAG
                     EWIANRFGGRENLEAIDFLRNFNTALFDAYPDCHTIAEESTSWPLVSRPVYAGGLGFD
                     LKWDMGWMHDTLAYFALDPIHRKYHHEKLTFRMLYASHENFVLPLSHDEVVYGKRSLL
                     SKMPGDEWQRFANLRLLFGYMYGQQGKKLLFMGAELGQEREWVHDDELDWELLDKPMH
                     AGTAHWLSDLNALYAGRLALHQRDCQPGGFEWIDCNDAETSVISFMRRSAAPLKTVVV
                     VCNFTPVPRHNYHIGVPQGGIWLELLNSDAQHYGGSGMGNCGRVEAAPVPLHGRSHAL
                     TLTLPPLAALFFAPEEEQG"
     gene            3348..>5603
                     /gene="treY"
                     /locus_tag="VMZ02_00785"
     CDS             3348..>5603
                     /gene="treY"
                     /locus_tag="VMZ02_00785"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HUR70487.1"
                     /translation="MDPKHTALEPELAPDDPTISVPNSTYRLQFTAAFGFRDALAILP
                     YLAELGIDTVYASPIFHARSGSTHGYDVVDPNHFNPELGTAEDFIALSDAARQQGLGW
                     LQDIVPNHMAYDGQNRMLMDVLEYGRASRFADTFDIEWNHPYESLKEKLLAPFLGAFY
                     GQCLENGDIQLRYDEAGFSINYYALRLPVQIASYGTILSAGLGALRRRLGGRHPDVLK
                     LLGVLYTLRSLPHEEDPRERADQSAFVKTTLWELYCSSAAIKEFIDSEVAGFNGTPHE
                     PASFDALDRLLSEQPYRLSFWKVAAEELNYRRFFNINELISVRAEDASVMMQTHGLIF
                     SMARQRRFTGLRVDHIDGLYDPAVYLARLRQEVPGAFIVVEKILALDEDLPDWPVAGT
                     TGYEFLNYAAGIFCARQNKKKFGQIYARFTGQQGSCHQLAFDKKRLIMGKYMAGDIDG
                     LARLLKTISSRDRHAADVTLYGLKRALVEVLTFFPVYRSYVSERSFSDADRQFIERAI
                     AKAKETNPGLLFELDFIERFLLLRFTNQSEEERKNWTHFVMRFQQLTGPLMAKGFEDT
                     TLYIYGQLLALNDVGGDPDRFGVTLDQFHRFNRRRAERWPHALNATATHDTKRGEDAR
                     ARMLVISELADQWERHLKRWSRLNRSKKEIHRGHEVPDTNDEYFLYQTLAGAWPLGGE
                     DRAGFLGRLKEYLIKAVREAKVHTEWLKPDLTYEDAYVRFVEAILEPGNDFVGELESW
                     CETLAFHGAINS"
BASE COUNT         1240 a         1539 c         1606 g         1218 t
ORIGIN      
        1 ctccaagggc gaggtggcgg cgaacgccgg cggaatcgaa attaagaagg tgagaaccca
       61 gatcgataaa cgcatgcaga tctcctgagc tcacgacggc gattttaaac caagcgccgt
      121 gagcgcccgc gattggtatc tggcacggtt ttgagcaaac cgaatgccac ccttcttaaa
      181 agcttcatgc gaacccaagc gcacgattgt gtcaaaagca ggatgagcct ctgagcggca
      241 ttgcggaaat gggacactga ctctggagaa tttggtaaag gatttctggc gcggtggtat
      301 agggtttgct cgaattcatc gcagatccaa accacggaga attctatgga aaatcaaccg
      361 taccgcgccc aggattttaa aatttccggc ctcaagggaa tatcggaccg caccgtagag
      421 atgcatctga aactttacga aggctatgtg aaaaatacca acatgctcaa tgaacacgtt
      481 gccgagttca ttcagaagaa aaaaaacgct gcgaccgatc ctggctatag tgagcttaag
      541 cgtcacctcg gtttcgagta cggtggtatg gtgctgcacg aacactactt cggcaatttg
      601 cgcccaggcg gaggcggagc gccgaccaag agtttcatca atgccaccga gatcggcttc
      661 ggcggttttg atgcctggaa agctgatttc gtcggcgtcg ggtccatgcg tggggtgggc
      721 tgggcggtcc tttatcagga tccagtgacg cacaatttat ccaatcactg gatcgaacta
      781 catcaacagg gcgtgcctcc gggcttcaaa ccgattcttg tcatggacgt ttgggagcat
      841 gcattcctgc tcgactaccc gcccagcgaa cgcagtaagt acatcgatgc tttctttgaa
      901 aatgtcgatt ggcaagttgt cgagaagcag ctcgggagcg ctagcgcgac ccggccggtt
      961 ggatgagtca cagcccggat taatctaatt atttattctt aatacgggcg gttcggttcg
     1021 cgccggcgtg attgcgcggc ggcgaacctg ttcacgtcag cggaggatct atggcgaaaa
     1081 atgagagtac cgatgggcgc gacccagcgc ggctgaagaa ggtcgtcaga atgcccaggc
     1141 ctaaagccgt aacgatgacg ctggaatatt ttgctcccga agccaatacg gtcgcggtgg
     1201 ccggcgagtt taacagttgg aacgagcaag cgcagatgat gaaaaagacc aaagccggat
     1261 ggtggaagac cagcttgaaa ctgccacccg gtcaatatca gtatcgcttt gtcgtcgacg
     1321 gccagcagtg ggtcgaggat cccgagaacc ccaataagca actcaacgaa ttttccgcat
     1381 acaattcggt cctcgaggtg cgatagcgag catggatgat gacatcgcgg cggcagattc
     1441 gggattcgtc agtgaacagg atctacgcct atttcgtgaa gggcgacacc accgtttgta
     1501 cgaaaaatgg ggcgcccatt tcggtgtgga ctcgcagggc gcctccggag tgtatttcac
     1561 cgtctgggcg ccgaacgccc aacgggtcag cttgattggc gattttaccg gctggaaaga
     1621 acaagaaggt ttgctgcgct gcctgggcga ctccggcgtt tggcagggtt tcgtgccggg
     1681 actgcacgcc ggcgcgcttt acaaatatcg ggtgtattcg cgccacttgg gatatcgcgc
     1741 cgataaggcc gatccgttcg cgttttacaa tgaaatcccg cccaagagcg cttcaatcgt
     1801 ctggggtctc gagtacacct ggggcgacga ggattggctg cgtcgacgca gggaacgcag
     1861 tgccctcgat aaaccgatgt ccatttacga aatgcatatc ggctcgtggc ggcgccggcg
     1921 cggcgaaaac aaccggtcac tttcctaccg ggagttggcg ccgctgctga ccgagcatgt
     1981 caagcgttta ggatttactc acgtcgaatt cctgccggtg atggatcatc cattttttgg
     2041 ctcttggggc tatcaggtca ccggctactt cgccgcctca ggacgctatg gcacgcccca
     2101 ggacttgatg ttcctgatcg ataccttgca tcagaacggc atcggcgtaa ttctcgattg
     2161 ggtgccgtca cacttcccga cggacgagca cggcctcgga tatttcgatg gatcccatct
     2221 ctacgagcat gccgacgcgc gccaaggcat gcaccgcgaa tggggcagtt acgttttcaa
     2281 ttacggccgc aatgaggtgc gcagcttttt gatcagcaac gggctgtttt ggctcgaccg
     2341 ctatcacgcc gacggactgc gtgtcgatgc cgtagcttcg atgctgtatc tcgactatgg
     2401 ccgttccgcc ggcgagtgga ttgccaatcg cttcggcggt cgcgaaaacc tcgaggcgat
     2461 cgattttttg cgcaacttca acacagcgct gttcgacgca taccccgatt gccacacgat
     2521 cgctgaagag tcgacttctt ggccattggt gtcgcgccca gtgtacgccg gcggtttggg
     2581 ttttgatctg aagtgggaca tgggctggat gcacgacact ctggcctatt tcgccctgga
     2641 tccgattcac cgcaagtatc atcacgaaaa acttacattc cgcatgcttt acgccagtca
     2701 cgagaacttt gtgctgccgc tgtcgcatga cgaagtggtt tacggcaagc gctccttgct
     2761 gagcaaaatg cctggcgatg agtggcaaag gtttgccaat ctcaggctgc tgttcggtta
     2821 catgtatggt cagcaaggaa agaagctgct gttcatgggt gcggaactag gccaagagcg
     2881 cgagtgggtg cacgatgacg agctcgactg ggagctgctc gacaaaccca tgcatgcagg
     2941 cacggcgcat tggctgagcg atctaaatgc actttacgca ggtcggctgg cgcttcatca
     3001 gcgcgattgt cagccgggcg gatttgagtg gatcgactgc aatgatgccg agacgagcgt
     3061 catcagcttt atgcgccgca gcgccgctcc actaaagact gtcgtcgtgg tttgtaactt
     3121 tactccggtg ccgcggcaca actatcatat tggcgtgccg caaggcggca tctggctcga
     3181 actgttaaat agcgatgcgc aacactacgg cggcagcggg atgggcaact gtggcagggt
     3241 cgaagccgct ccggtgccgt tgcatggccg cagtcacgca ttgacactga cgctgccgcc
     3301 attggcagcg ctgttcttcg ctcccgaaga agaacaaggg taagccgatg gaccctaaac
     3361 acacagctct tgaaccggag ctcgctcccg acgatccgac gatctcggta ccaaactcca
     3421 cttatcgcct gcaatttacc gcggcgttcg gtttccgcga tgcattggcg atcctgccgt
     3481 atctcgcaga gctcggcatt gacaccgtct atgcctcacc gatctttcac gcccgcagcg
     3541 gcagcacgca cggctatgac gtcgtcgatc cgaatcattt caatccggaa ctgggtacag
     3601 cggaggactt catcgctttg agcgatgcag cccgccaaca gggtcttggc tggttacagg
     3661 acattgtccc caaccatatg gcctatgacg gtcagaaccg catgctcatg gacgtgctgg
     3721 agtacgggcg agcgtcgcgg tttgccgaca ctttcgacat cgaatggaat catccctacg
     3781 agagcctgaa agaaaaactg ctggcgccgt ttctcggcgc attctacggc caatgtttgg
     3841 aaaatggcga cattcagttg cgctatgacg aagcgggatt cagcattaat tattacgcgc
     3901 tgcgcttgcc ggtgcagatc gcgtcctatg gaaccatctt gagtgccggg cttggcgcgc
     3961 tgcgccgccg gcttggcggg cgccatccgg acgtgctgaa acttttgggt gtgctctata
     4021 cgctcaggag cctgccccac gaagaagacc cgcgcgaacg tgccgatcaa agcgcattcg
     4081 taaaaactac cttatgggag ctctactgca gcagtgccgc gatcaaagag tttatcgaca
     4141 gcgaagtcgc cggcttcaac ggaacgcccc atgaaccggc gagtttcgac gcattagatc
     4201 ggctcctcag cgagcaacct tatcgcttgt cgttttggaa ggtcgccgcg gaggaactga
     4261 actaccggcg tttcttcaac atcaacgagt tgatctcggt acgtgccgaa gatgcctcgg
     4321 tgatgatgca gacgcacggt ttaatttttt caatggcgcg ccagcggcgc ttcaccggat
     4381 tgcgcgtcga tcacatcgat ggactctacg acccggcggt ctacctggcg cgtttgcgcc
     4441 aggaggtacc gggagctttt atcgtggtcg agaaaatcct cgcgctcgat gaagatttgc
     4501 cggattggcc ggtggcgggg accacgggtt acgagttttt gaattatgcc gcgggaattt
     4561 tttgcgcgcg ccaaaataag aagaagtttg gccagatata cgcccgcttc accggtcaac
     4621 aaggctcctg ccatcaactc gccttcgaca agaaacggct aatcatgggc aagtacatgg
     4681 ctggggatat cgacggcttg gcgcgtttgt taaagacgat ttccagccgc gaccgccacg
     4741 ccgcggatgt gacgctttat ggcttgaagc gggctctggt cgaagtgttg acattttttc
     4801 cggtttaccg cagttacgta tccgagcgca gcttcagcga tgccgaccgg caatttatcg
     4861 aacgcgccat cgcaaaagcc aaggaaacca atccgggctt gttgttcgaa ctcgatttca
     4921 tcgagcgatt tctattgctt cgtttcacca atcagagcga agaagagcgc aaaaactgga
     4981 cccatttcgt catgcgcttc cagcaactga ccggaccgct catggcgaaa ggcttcgaag
     5041 acacgacgct ttacatctat ggccaactgc tggcgttgaa tgacgtcggc ggcgatccgg
     5101 atcgcttcgg tgtgacgcta gatcagtttc atcgttttaa ccggcgccgc gccgaacgct
     5161 ggccgcacgc tctcaatgcc acggcgaccc atgacaccaa gcgcggcgaa gatgcccggg
     5221 cgcgcatgct ggttatctcc gagctcgccg accagtggga acgtcatctc aagcgttgga
     5281 gccggctcaa ccgcagcaag aaagaaatcc atcgcggcca cgaggtaccc gacaccaacg
     5341 acgaatattt tctctatcag accctcgccg gcgcctggcc cctcggcggc gaggatcgcg
     5401 ccggctttct cggcagactc aaagaatatc tcatcaaggc cgtgcgcgaa gccaaggtcc
     5461 ataccgagtg gcttaaaccg gatctcacct atgaggatgc ctacgtgcgc tttgtcgaag
     5521 cgatcctcga gcccggcaac gattttgtcg gcgaactcga aagctggtgc gagacactgg
     5581 catttcatgg cgcgatcaat tca
//
LOCUS       DATLCH010000029         4421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7508516, whole genome shotgun sequence.
ACCESSION   DATLCH010000029 DATLCH010000000
VERSION     DATLCH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7508516"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            457..1362
                     /locus_tag="VMZ02_00790"
     CDS             457..1362
                     /locus_tag="VMZ02_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HUR70488.1"
                     /translation="MAFQRVGIMSIGEMGFHWAKLLKSHGVEVLTYDKDRGEVSRKRA
                     ENAGVTSVGSMNDLVQQAEVIVSIVVPSAAKKVADQVAIAARATDRKGLLYCDANAIS
                     PMTADAIANVLEPAAVNFVDGCIIGSAAKMGKGSIVYLSGPQAQCLQGLEAFNIPLKI
                     LGTGTNQASAFKVVYAGLTKGLQGLFCELLMGARRFGLLDEIRAQYDESFPGLLDKIS
                     SSIVGLQIHAGRRAEEMDELERTFNAQGLESYMAPATQKVLQAIAALKLGQASDSGAR
                     HGELTETLELFYQTGLLQRKSPDRP"
     gene            1423..3192
                     /locus_tag="VMZ02_00795"
     CDS             1423..3192
                     /locus_tag="VMZ02_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUR70489.1"
                     /translation="MSRVEKKQPEYGSDVVVDLMKAHDIEYTAFNPGATFRGIHDSIV
                     NYGGNYKPEVIFCHHEEISVAIAHGYAKAKNKPMIAIVHNMVGLQHASMAIFNAYIDR
                     VPMMVLGGTGPMVTKRRRPRIDWIHTALVQGNQVRDYTKWDDQPYSLADVPDSFIRGY
                     RIATTDPTAPVYINYDADIQEDAVKALVDMPDVSRHSKPAPAQANPEALRRAAEMLVN
                     AKTPLIVADMLGRNPKAVPLLIELAEVLAVPVVDRGARFNFPAVHPLDVTDGAREILQ
                     KADFILALDVGDLFGALTTVSKQTRESQYVTSPGVKIASISMNDMLVHSWANDFQALQ
                     AIDIPMCADTSVALPELTRLCREMIGNNSATKAIIEARQKELTEKHKSRRAKWLADAQ
                     AKGSQKEISTAWLALEIGEAIKREDWVCVNGTSNGWTRRLWDFSKPNQSLGGSGGAGL
                     GYGPGAAIGAALALKGSGKLAVAIQSDGDMLMTSSAIWTAAKHRIPLLMIMHNNQSYY
                     NSEEHGIEVAKFRKRPVENAPIGTHVDDPEIDFAMMAKSFGVNSDGPIRNPADLRPAI
                     ERGVKYVKEQQKPYLVDVIAEPR"
     gene            3281..4141
                     /locus_tag="VMZ02_00800"
     CDS             3281..4141
                     /locus_tag="VMZ02_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR70490.1"
                     /translation="MSASPDDRTVVTDSYRDFRQAVEAPEERIDLGRAALTIALADYP
                     DLDISKYLGRIDELAIQVSQRSGADADIFRSIAALNYVLFTEQGFGGNREDYYDPKNS
                     FLNEVIERKRGLPITLSILYMEVGQRIGITIEGVGFPGHFLVKTKSEDDEIVIDPFNS
                     GDIKSHEELQIMLDQIYSGKVRLHRELLAALPKKQILKRMLGNLKAIYGRAEEWVKLI
                     AVLDRLLILDPDSVENLRDRGVVYRRLDCYGQAKDDFETYLRLAPNAQDAAAIREQLS
                     ELAKHTILIH"
     gene            complement(4240..>4421)
                     /locus_tag="VMZ02_00805"
     CDS             complement(4240..>4421)
                     /locus_tag="VMZ02_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70491.1"
                     /translation="RVIYSRQELQRIWSESVKNLKARRLSAIRPMQLTRAALHDLFLT
                     RSFAADFKEFSKLGA"
BASE COUNT         1025 a         1168 c         1273 g          955 t
ORIGIN      
        1 gatgagccgg gacctaaata taagcggatc aattgtcgct caatcgagca taagacatgc
       61 catccccgat ggatgaaagg ggcggcaaaa cggagttgat ttaacaagaa aagccgatcc
      121 ggcgcggctt caatcgccta gcaggacccg caacgatgga cggaattgaa caggcctaac
      181 aaatctgcgg atcatccaga ctgtttcgcc gcgttctttc actcctgtca ggagcgtgct
      241 ttggacggga ttgtcctaca acattcctga agggattctc actttgatcc tgaatttata
      301 agttcgaatt ccctgatgga aagaactatt gccgggtctt cccatggccg caatcagtgt
      361 tgccgacagc gagtatgttg gctgatcgcg ttgcctttaa gcgcgctgac cgatatggca
      421 tagagagcgt cgaacgcgct aaggaggcgg aagattatgg catttcaaag agtcggtatc
      481 atgagcatcg gcgagatggg ttttcattgg gccaaattgc tcaagagcca tggtgtcgaa
      541 gttttaacct acgacaagga ccgtggcgag gtgagccgca agcgcgccga gaacgccggg
      601 gtgacatcgg ttgggtcgat gaacgacttg gtgcagcaag ctgaagtgat cgtttctatt
      661 gtcgtgccgt cggcggccaa gaaggtcgct gatcaagtgg cgattgccgc ccgcgccacc
      721 gatcgaaagg gtttgttgta ctgcgatgcc aacgcgatct cgcccatgac cgcagacgca
      781 atcgccaacg ttctcgaacc tgccgcagtg aattttgtcg acggctgcat catcggctcc
      841 gcggcaaaga tgggtaaagg ctcgatcgtt tacctttccg gaccgcaagc acagtgcctg
      901 caagggctag aagctttcaa cattcctctc aagattttgg gcaccggtac aaatcaagcc
      961 tcggcattca aagtcgtcta cgccggtctt accaaaggat tacagggact gttttgcgag
     1021 ctgctcatgg gggcgcgccg cttcggtttg ctcgacgaga ttcgcgctca gtacgacgag
     1081 agctttcccg gtttactcga caagatttcc tcgagcatcg tcggattgca gatccacgcc
     1141 ggccggcgcg ctgaggagat ggacgagctt gaacgaacgt tcaatgccca aggtctcgag
     1201 tcgtatatgg cacctgcgac tcagaaggtg ttgcaggcga tcgctgcgct caaactaggt
     1261 caagccagcg acagcggagc gcgccacggc gagctgacgg aaaccttgga gttgttttat
     1321 caaactggtc ttttgcagcg gaaatcgcca gatcggccct agccaggcgc cggctttcct
     1381 ggtattgaga gcaaaacagt aattcgcagg aggagtttag aaatgtccag agtagaaaag
     1441 aaacaaccgg aatacggttc ggatgtcgtc gtcgatttga tgaaagcgca cgatatcgag
     1501 tacacggctt tcaatcccgg cgcgactttc cgtgggattc acgattcgat cgttaactat
     1561 ggcggcaatt acaagccgga agtgatcttc tgccaccacg aagaaatctc ggtcgccatc
     1621 gcgcatggtt acgcgaaggc gaaaaataag ccgatgatcg ccatcgtgca caacatggtc
     1681 ggcttacagc atgcaagtat ggcgatcttt aacgcataca tcgatcgcgt accgatgatg
     1741 gtgctcggcg gcaccggacc gatggtcact aaacggcgcc gaccgcgcat tgactggatt
     1801 cacaccgctc tggtccaagg taatcaagta cgcgactaca ccaagtggga cgatcagcct
     1861 tattcgttgg ccgacgtgcc ggattcgttt atccgcggct accggatcgc caccaccgat
     1921 ccgacggcgc cggtgtacat caactacgac gccgacattc aagaagatgc ggttaaggcg
     1981 ctggtcgaca tgccggacgt gagccgtcac tctaagccgg cgccggcgca agccaacccg
     2041 gaagcgttgc ggcgcgcggc ggaaatgctc gtcaacgcca aaacgccgtt gatcgttgcc
     2101 gatatgcttg gccgtaatcc caaggcggtg ccattattga tcgaactcgc tgaagttctc
     2161 gccgtgccgg tcgtggatag aggtgcgcgt tttaattttc cagcggtgca cccgctcgat
     2221 gttaccgacg gcgcgcgtga aattctccag aaagcggatt ttattttggc tctcgatgtc
     2281 ggcgatctgt ttggcgcact gacgacggtg agtaagcaga cacgtgaaag ccaatacgtc
     2341 acgtcgccgg gggttaagat cgccagcatc tcgatgaatg atatgttggt tcactcctgg
     2401 gccaacgact ttcaggcgct tcaagcgatc gacattccga tgtgcgccga cacttcggtc
     2461 gcgttaccgg aattgactcg gctgtgccgc gagatgatcg gcaataacag cgctaccaaa
     2521 gcgatcatcg aggcgcgcca aaaggaattg actgaaaagc acaaaagtcg ccgggctaaa
     2581 tggctggcgg atgcgcaagc caaaggttcg cagaaagaaa tttcgaccgc ctggttggcg
     2641 ttggagatcg gcgaggccat taagcgtgaa gattgggtct gtgtgaatgg tacgtcgaac
     2701 ggttggacgc gccggctgtg ggatttcagt aaacccaatc agtcactcgg cggcagcggc
     2761 ggcgctggtc tcggatacgg tccgggcgcc gcgatcggtg cagccttggc gcttaagggc
     2821 agcggcaaac ttgccgtggc gatccagtcc gatggcgaca tgctgatgac ttcgagtgcg
     2881 atctggaccg cggcgaagca ccgtattcca ctgctcatga tcatgcacaa taaccagtcc
     2941 tactataatt ccgaagaaca cggcatcgaa gttgccaaat ttcgtaaacg accggtcgag
     3001 aatgcgccga tcggcaccca tgtcgatgac ccagagatcg acttcgcgat gatggcgaaa
     3061 tccttcggcg ttaatagcga cggaccgatc cgcaaccccg cggacctgcg cccggctatc
     3121 gagcgcggcg tcaaatacgt caaagagcag cagaagccgt atctggtcga cgtcatcgca
     3181 gagccgcgct agagaagttg acggaggcac gttacggcgg aggcgccgag ttgcagagtt
     3241 gcgaaggaat gcccttccct gttttctcgg cgtctctgcg gtgtcagcat cccccgacga
     3301 ccgcacagtg gttacagatt cttaccgaga ttttcgccag gcagttgagg ctcccgagga
     3361 acgcatcgat ctcggccgcg ccgcattgac tatcgcgctc gcggattatc ccgatctcga
     3421 catatccaaa tacctcgggc gtattgatga gttggccatt caggtaagcc agcgctcagg
     3481 cgcggacgcc gacatttttc gctcgatcgc cgcgctcaac tacgtgttat tcacggagca
     3541 gggctttggc ggcaatcgcg aagattatta tgatccaaag aacagctttc tgaatgaggt
     3601 gatcgagcgc aagcgcggac tgccgatcac tctatcgatt ctttatatgg aagtaggaca
     3661 aagaatcggt attacgatcg agggcgtggg ttttccgggc catttcttgg tcaagaccaa
     3721 gtccgaggac gacgagatcg ttatcgatcc gttcaattcc ggcgacatca agtctcatga
     3781 agagcttcag atcatgctcg atcaaattta cagcggtaaa gtgcggcttc atcgggagct
     3841 tctcgctgcg ctaccaaaga aacagatact caagcgcatg ctcggcaatc tgaaggccat
     3901 ctatggccgc gccgaagagt gggtcaaatt aattgccgta ctcgatcgtc tgcttattct
     3961 cgatcccgac tcggtggaga atttacgaga tcgcggcgtg gtctaccggc ggcttgactg
     4021 ttacggccag gcgaaagacg attttgagac ctatctgcgc ttggcgccca acgcccagga
     4081 cgccgcggcg attcgcgagc agctgagcga gttagcaaag cacactattc tgattcacta
     4141 acgcgcgtat tgggcgcatt ctagctggct aattaaccga ggtcgttaga tgggcgagga
     4201 ggattgtccg cagttatgat tgggtcagcg gcggatcctt tatgctccca actttgaaaa
     4261 ttctttgaag tcggcggcga aagagcgggt cagaaacaag tcatgcagcg ccgcgcgagt
     4321 tagttgcatg ggtctgatcg ccgaaagcct acgagccttg agatttttca cgctctcgct
     4381 ccagatccgc tgaagttcct ggcgcgaata gattacccga t
//
LOCUS       DATLCH010000030         5274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_858371, whole genome shotgun sequence.
ACCESSION   DATLCH010000030 DATLCH010000000
VERSION     DATLCH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858371"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VMZ02_00810"
     CDS             <1..204
                     /locus_tag="VMZ02_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70492.1"
                     /translation="SLLNVYSYPRSPNVIVVYTAEIIGGTLTAADESTDAAVFAADDV
                     PWHELAFASTKDALKDYVRLYLS"
     gene            complement(347..655)
                     /locus_tag="VMZ02_00815"
     CDS             complement(347..655)
                     /locus_tag="VMZ02_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR70493.1"
                     /translation="MANFVKVCKTDDVKGGCGKSLEVNGKAVGVFNIDGKFYAINDVC
                     GHRGGPLAEGEVDGTTVICPWHGWRYNVTTGENELLPALPTQKYEVQVEGDDVLVDLG
                     "
     gene            complement(720..1871)
                     /locus_tag="VMZ02_00820"
     CDS             complement(720..1871)
                     /locus_tag="VMZ02_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HUR70494.1"
                     /translation="MIKCPLSALSMLGPDGIFFESMAKRSTLTDLHESRGAVFAEVDG
                     WLLPQHFGDAASEYDTVRQSAGLFDLADRAMLQFTGDDRLSYLQGMLSNDLRPLQTFD
                     GEQAAILTQQGKVIADVRVLCSLNSFYLDFCEPLKEKILTHLNRYLVADDVEIHDPNE
                     EWKMLSVQGPKAQMLLDQLFANAELPGQAHHHAMVQFDGSPVCVIRANRTGYDGFDLI
                     VQGTQITTFAQRLVELGAGWVGAQAQNILRIEAGIPRYGIDFSADNLLLEVALDDAVS
                     FNKGCYLGQEVIERIRSRGHVNKKLCGLLLDGDNPVSPGDTLSAGDKAVGQITSSVVS
                     IALRRPIALAYVSKDFWTPGTTVSVHHSGGEIGATVTSRPIVAVHPAGT"
     gene            1929..2396
                     /locus_tag="VMZ02_00825"
     CDS             1929..2396
                     /locus_tag="VMZ02_00825"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:2061..2063,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUR70495.1"
                     /translation="MAGLEGQKAPAFTLAGSDGKQHSLADYAGKNVILWFYPKDDTPG
                     UTKEACGFRDAIDTVTKANTVVIGVSPDGIDSHNQFISKFNLPFVLLADQQKETMKAY
                     GAWGPRKNAQGEVSEGTIRSTALIGPDGTVKKHWIPVADAGAHPAEVLEFLNK"
     gene            2473..3384
                     /locus_tag="VMZ02_00830"
     CDS             2473..3384
                     /locus_tag="VMZ02_00830"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HUR70496.1"
                     /translation="MAKNKADLDLHKLEIFYWVAEHKSFSQAAELLSLRQPTVSAHVQ
                     ELEKTIGGKLLYRMPGKVSLTPLGLMLAERAKNLLSFKRETVAAVELFHGTLSGELWV
                     GGSSIPGEYLLPRKLGEFARKYPRVKPILRIGDSAGIVTDLLEGKVEIGFVGFRGDDT
                     RLLFEKIWDDEMVVAVPRGHPWTRRKSIEVADLRTENFISREHGSGTLDSLRQLLAKR
                     KTPNQDLLKISMELGSTEAVKEALMAGYGFSILSRISIQHELADGNIVEVPIRSLKME
                     RGFYQVFHSRRPLHPIAQTFRNFIKQK"
     gene            3478..4842
                     /locus_tag="VMZ02_00835"
     CDS             3478..4842
                     /locus_tag="VMZ02_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUR70497.1"
                     /translation="MALSLKRHDPSAAEQLRERLIAAWTRSDKLFALMGAGQALTKPI
                     VWRHPFIFYIGHLPAFSWNQICGGLLNWPSLNSYFDDLFCRGIDPDVDTGECHWHPEV
                     PDQWPTLAATHEYRDKVRLALLDSTDAIAYCHSKDPLAHGGRVFELILEHEYMHQETL
                     MYMLQQVSIDAKLRPSDLPPYAFGRATAREAVRISAGKARLGARFEEIAFGWDNEFSE
                     VVVNVPQFHMDSLPVTNGEFLEFVGAGGYNEPRYWRPADWRWKELEQKRHPTGWLKNE
                     KNWCFRAMFDLLPLEKVANWPAYVSLAEARAYAAWRGLRLPTEAEYHRAAFYGPNERE
                     ARYPWGDAEPNTRHGNFDFTAWSPQPVGSHPDGASRWGVQELIGNGWELTDTPFAPLP
                     GFTPYMTTYPDYSKDFFDGKHFVVKGASWATDRQLLRPSFRNWYQAHYPYMFAKFRCV
                     GEID"
     gene            4858..5139
                     /locus_tag="VMZ02_00840"
     CDS             4858..5139
                     /locus_tag="VMZ02_00840"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HUR70498.1"
                     /translation="MKALWENAVIAQSNETIVVEGNHYFPPDSIDTAYFAPSDTHTSC
                     HWKGIASYYHVKIGEKVNADAAWYYPEPKDAARSIKNYIAFWRGVKIVE"
BASE COUNT         1210 a         1425 c         1433 g         1206 t
ORIGIN      
        1 tccctgctca acgtttattc gtatccgcga tcgccaaacg tcattgtggt ttatacggct
       61 gaaattatcg gcggcacgct gaccgccgcc gacgaatcca ccgatgccgc ggtctttgca
      121 gcggacgacg tgccgtggca cgagcttgct ttcgcgagca ccaaggatgc gctcaaggat
      181 tatgttcgtt tgtatttgag ttaaatatct agaccgcctc gagcctacgt gcgcgggata
      241 acctgatata gagcttactt atggggccgc tttccagcgt gttcgcaaaa taaaaaaaag
      301 gcccgctagc tgattaagct gcgggcctgg tcgttgaatg atattctcag cctaggtcga
      361 ccagcacgtc atcgccttcg acttgcactt catacttctg cgttggcaac gcagggagca
      421 gctcgttctc accggtggtc acgttgtagc gccagccgtg ccatggacat atcaccgtcg
      481 tgccatcgac ctcgccttcg gctaagggac cgccgcgatg accgcaaaca tcattgatcg
      541 catagaattt cccgtcaatg ttgaagacgc cgactgcctt accgttgacc tctaaacttt
      601 tgccgcagcc gcctttgaca tcgtcggttt tgcagacttt tacgaaattg gccattaccc
      661 caagttcctt tctgatccgg aacaaatttc cgcttaatat acggagttga agcttcgttt
      721 caagtgccgg cgggatggac ggcgacaatc ggcctcgacg ttacggtcgc accaatttcg
      781 ccgccgctgt ggtgcacgga caccgtcgtg cccggggtcc agaaatcttt gcttacatat
      841 gccaatgcga tcggccttcg gagcgcgatc gagaccactg agctggtgat ctgacccact
      901 gccttgtctc cggcgcttag cgtgtcacct ggactgaccg gattgtcgcc atcgagcagg
      961 agcccacaaa gttttttgtt tacatgtcca cgcgaacgga tgcgctcgat gacctcctgg
     1021 ccgaggtagc agcccttgtt gaagctcacg gcgtcatcga gggcgacctc aagaagcaaa
     1081 ttgtcagcgg aaaaatcaat gccatagcgg ggaatgccgg cttcgatgcg tagaatattt
     1141 tgcgcctgcg caccgaccca tcctgcgccc agttcgacca gccgctgcgc aaaggtcgtg
     1201 atctgtgtgc cttgtacaat caaatcaaaa ccgtcgtagc cggtccggtt ggcgcggatg
     1261 acgcacaccg ggctaccgtc gaattgcacc atggcgtgat gatgcgcctg cccgggcagc
     1321 tcagcattgg caaatagctg gtcgagcagc atctgcgctt tcggcccctg gaccgaaagc
     1381 atcttccact cctcattggg atcatggatt tccacatcat cggcaactag ataacgattc
     1441 agatgagtta agatcttttc tttgagcggt tcgcaaaaat cgaggtaaaa agaattcaaa
     1501 gagcacaata cccggacatc ggcgattact ttgccttgtt gagtgaggat cgccgcctgt
     1561 tcaccgtcaa acgtctgcaa cggccgcaga tcgttggaca gcataccttg caggtaggac
     1621 agccgatcgt cgccagtgaa ttgaagcatg gcgcggtcgg cgagatcgaa taggccggcg
     1681 gactggcgta ccgtgtcgta ttcactggcg gcatcgccga aatgttgcgg caggagccag
     1741 ccgtccactt ctgcgaacac ggcccctcgg gattcatgca aatcggttag tgttgatcgc
     1801 tttgccatgc tttcgaaaaa tataccgtct ggacccagca ttgacaatgc cgacaatggg
     1861 cacttgatca ttgatagata gattaccggt gtgatagaca aacaatatct ttaatacgga
     1921 ggtgcttgat ggcgggactt gaagggcaaa aggcgccggc gttcactctt gcagggagtg
     1981 acggcaaaca acattcgctg gctgattacg caggtaaaaa cgtaattctg tggttttatc
     2041 cgaaagacga tacgcctggc tgaaccaaag aagcgtgcgg gttccgcgac gctatcgata
     2101 ctgtaacgaa agccaatacc gtggttatcg gcgtaagccc ggatggcatc gattcgcata
     2161 atcaattcat tagcaagttt aatctgccct ttgttctcct cgccgaccag caaaaagaaa
     2221 ccatgaaagc ttacggcgct tggggtccac gcaagaatgc ccagggcgaa gtgtccgaag
     2281 ggacgatccg ctccaccgca ttgatcggcc ccgacggcac agttaagaaa cattggattc
     2341 ctgtggccga tgccggtgct catccggccg aagttttgga atttctaaat aagtaaatcg
     2401 tcttcgttcc gctcggggag cgcgccgttc gattgaccgg tagcgctccc tgggcagccc
     2461 gtttcagctt tcatggctaa aaataaagcc gatctcgatc tccataagtt agaaattttt
     2521 tattgggttg ccgagcacaa gagtttttcg caagcggccg aactactttc attgcgtcag
     2581 ccgaccgtga gcgcccatgt tcaggaactc gaaaagacca tcggcggcaa gctgctctat
     2641 cgcatgccgg gaaaagtcag ccttactcct ttaggtctca tgctcgccga gcgggcgaag
     2701 aatttgctca gtttcaaacg cgaaacggtt gcggcggtgg aactgtttca cggcactctc
     2761 agtggcgagc tttgggtggg cggtagcagc attcccggcg aatatctgct gccgcgaaaa
     2821 ttaggcgagt ttgcccgcaa atatccacgc gtcaaaccga tcctgcgcat tggcgactcc
     2881 gccggcattg tcaccgatct actcgaggga aaagtcgaaa tcggtttcgt cggatttagg
     2941 ggcgacgaca caagattact gttcgagaag atctgggacg acgagatggt cgtcgcggtc
     3001 ccgaggggtc atccatggac gcggcgcaaa tccatcgagg tcgccgatct gcgcaccgag
     3061 aactttatct cgcgtgaaca tggctcgggc actttggatt ccctgcgcca gctgctggct
     3121 aaacgtaaga cacctaatca agacctcttg aaaatcagca tggaactcgg cagcaccgaa
     3181 gcggtcaaag aagcattgat ggccggttac gggttttcaa tcttgtcgcg tatatccatc
     3241 caacatgaat tagcagacgg gaacattgtc gaagtgccga ttcgatccct taagatggag
     3301 cgtggcttct atcaggtgtt tcatagccgt cgaccgctgc atcccatcgc gcaaaccttt
     3361 cgcaacttca ttaaacaaaa ataaacgcgt ccaatttttc ctaaaaaact cgtgcgtggt
     3421 cttcaatagc caatgcctat ttagtatata gctagtagga atggggagac ttaggccatg
     3481 gcactatcgt taaaaagaca cgatccaagc gcggccgagc agttgcggga gaggctgatt
     3541 gccgcgtgga cccggtcgga caaactattc gcgctgatgg gggccggcca agcacttacc
     3601 aaaccgattg tttggcgcca cccgttcatt ttctatatcg gtcacttgcc ggcgttttcg
     3661 tggaatcaaa tatgcggcgg cctgctaaat tggccgtcac tcaacagcta tttcgacgat
     3721 ctgttctgcc gcggcatcga tcccgatgtc gataccggcg aatgtcattg gcatccagaa
     3781 gtgccggatc agtggccgac cctcgctgcg acgcatgaat atcgcgacaa agtacgacta
     3841 gcgcttttag attcgacgga tgcgatcgcc tattgccatt ccaaagatcc actggcacac
     3901 ggcggccgcg ttttcgaatt gatcttggag catgaataca tgcatcaaga gacattgatg
     3961 tatatgctgc agcaggtgtc gatcgacgcc aagctccggc ccagtgacct gccgccgtac
     4021 gctttcgggc gtgcaaccgc gcgcgaggcc gtgcgaattt cggccgggaa agcccgcctc
     4081 ggagcgcgtt tcgaagaaat tgccttcggt tgggacaatg agttctccga ggtggtcgtg
     4141 aatgtgccac agtttcacat ggacagcttg cctgtgacga atggtgaatt tctcgaattc
     4201 gttggcgccg gcggctataa cgagccgcgc tattggcggc ccgccgactg gcgctggaag
     4261 gagttggagc agaaacggca tccaacgggt tggcttaaga atgagaaaaa ttggtgcttc
     4321 cgcgccatgt tcgatttgct gccgctcgaa aaagtagcga attggccggc atacgtcagc
     4381 ttagccgaag cccgtgccta tgcagcgtgg cgtggtctac gtttgcccac tgaagcggag
     4441 tatcaccgcg ccgcattcta cgggcccaat gagcgtgagg cgaggtatcc gtggggtgat
     4501 gccgagccaa atacccgcca tggcaacttc gattttaccg catggtcgcc gcagccggtc
     4561 ggttcccatc ccgatggggc gagccgctgg ggagtacaag aactcattgg caatggctgg
     4621 gagttgaccg acacgccatt cgctccgcta ccgggattca caccttatat gacgacgtat
     4681 ccagactatt caaaggactt cttcgacggc aaacattttg tcgtcaaggg tgcatcgtgg
     4741 gccaccgaca ggcagctttt acggccgagc tttcgtaact ggtaccaagc gcactatcct
     4801 tacatgttcg cgaagtttcg ctgcgtcggc gagattgatt agattgaaag gataacaatg
     4861 aaagcgcttt gggaaaacgc cgttatcgcg cagtccaatg aaaccattgt ggtcgaaggc
     4921 aatcattact ttccgccgga ttcgatcgat acggcgtact tcgctccgag tgacacccac
     4981 acaagttgcc actggaaggg tatcgccagt tactatcacg tcaagatagg tgagaaagtc
     5041 aacgccgacg ccgcttggta ctatcccgaa cccaaggacg ccgccaggtc gatcaagaac
     5101 tatattgcat tctggcgtgg cgtcaaaatt gtcgagtaga tcgcgtctgc atgaaagtct
     5161 tgaagctcgc ctcctgcatg gcagagaata ccgacgcact ttgcgattcc gtcgcggcgc
     5221 tcctggatac cgagttgggc atccgcaccg aatacgtcac gggtatcccg tggc
//
LOCUS       DATLCH010000031         4867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6019995, whole genome shotgun sequence.
ACCESSION   DATLCH010000031 DATLCH010000000
VERSION     DATLCH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6019995"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VMZ02_00845"
     CDS             complement(<1..291)
                     /locus_tag="VMZ02_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70499.1"
                     /translation="MKSSDSGQLQLAVSDALVFRSFEADARARGFKAAAGVDEVGRGP
                     LAGPVVAAAVILPRGFTHPDIKDSKMLSAQQREKLVPIIQESAQCWAVGMIDV"
     gene            411..707
                     /gene="gatC"
                     /locus_tag="VMZ02_00850"
     CDS             411..707
                     /gene="gatC"
                     /locus_tag="VMZ02_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HUR70500.1"
                     /translation="MKLTRESVQRVATLARLRLTADEETALAGELDRILGYMDKLNDL
                     DTSNVPVFAHSNDVSEILEKLRDDKVVNRPNTDILLANAPERDKTFFRVPKIIE"
     gene            704..2161
                     /gene="gatA"
                     /locus_tag="VMZ02_00855"
     CDS             704..2161
                     /gene="gatA"
                     /locus_tag="VMZ02_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HUR70501.1"
                     /translation="MSLSALTLQQASEKLRRREISSVELTEAVFQRISAADDKIHAYL
                     TLARESALEQAKQADARRNSATQLSPLLGIPIAVKDNFLTRGVRTTCASKILGDFMPP
                     YDATAIVKLHAAGAVIVGKTNLDEFAMGSSAENSAFHPTRNPWNLGRIPGGSSGGSAA
                     AVAADECLAALGTDTGGSIRQPAACCGIVGLKPTYGRVSRYGIIAFASSMDQVGPMTK
                     DVSDAACLLQVLAGHDPADSTSSIRSVPDYSAALNGDVKGICIGVPKEYFVSGIQPEV
                     EKSVRAAVQELAKNGARIEEISLPHTEYAVAVYYVVATAEASSNLARYDGMRFGHRAE
                     AKDLTETYMITRAEGFGAEVKRRIMLGTYVLSHGYYDAYYLKAQRVRTLIRRDFDNAF
                     THCDVIVTPTAPTTAFKLAEKTQDPLQMYLSDIYTISTNLAGLPALSLPCGFDNDNLP
                     IGLQMIGKHFDEATLLRAAHAYEQATDWHKKTPTL"
     gene            2280..3725
                     /gene="gatB"
                     /locus_tag="VMZ02_00860"
     CDS             2280..3725
                     /gene="gatB"
                     /locus_tag="VMZ02_00860"
                     /inference="COORDINATES: protein motif:HMM:NF004015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HUR70502.1"
                     /translation="MKYEPVIGLEVHAQLLTASKIFCGCSTQFGGEPNAHTCPVCCGF
                     PGVLPVLNKKVVEFAIKAGLATHCEITRASILARKNYFYPDLPKGYQISQYELPICCD
                     GYIEFTLNDTLKRVRLTRIHMEEDAGKNIHDAHGDSSLIDLNRAGVPLLEIVSEPDMR
                     TTEEAGAYLRALRAILQFLEVCDGNMEEGSFRCDANISVRPAGTETLGTKIEIKNLNS
                     FRAVERALEFEIDRQSDTLNEGGTLAQETRLWDEHREETRSMRSKESAHDYRYFPDPD
                     LLPLVIPESWIEEIRAKLPELPAARKRRFITDHCLPAYDAELLTSRKDIADYFESAVR
                     VYSNAKSLSNWIVGDLFRVLKERKLDEQLYIDTWPIQAPHLAELVQLIDRGRINGKIA
                     KMVFESMLNSDDKTPQQLVNEKGLEQVSDAGSIEAAIDQVLTSNPKQVEQYRSGNDKV
                     FGFIVGQVMKATQGKANPQKVNEILRDKLNR"
     gene            complement(3771..4361)
                     /locus_tag="VMZ02_00865"
     CDS             complement(3771..4361)
                     /locus_tag="VMZ02_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUR70503.1"
                     /translation="MRSPITVPPTPNGKAEAKPIEIASAPPAFFQSALTEVPVAPPLL
                     VSGQPLISSRPAAIEAPTRPFVPASQVGIEKPSVNPIPEQLASLPNKPIEAAPAKAVV
                     ARPASPKPLEGFIIQLAFNDKERAQRWAEGMEKKGFAVSVTEAGNDGALRVRLGNFVL
                     RDDAERQLKAIKQDGLAGIILNLPQAFRPEARTSIP"
BASE COUNT         1115 a         1381 c         1311 g         1060 t
ORIGIN      
        1 gacatcgatc ataccgaccg cccaacattg cgccgactct tgaatgatcg gcaccagctt
       61 ttcgcgttgc tgagcgctga gcatcttcga atctttgatg tccggatgag taaacccgcg
      121 cggcaatatc actgccgccg ccacgaccgg accggcaagc ggtccgcggc caacttcgtc
      181 gactccggca gcggctttaa agccgcgcgc ccgagcgtcg gcttcgaatg agcgaaatac
      241 cagggcatcg ctcaccgcca actgcagctg tccactgtcg gaagatttca tcggtcttca
      301 tacttagcgc gaaacagcca gaaactgcaa attatttttg ccaaccgtcg cgagagtgtc
      361 cggtccacat gaggtggcaa tagcgtgacc catgtgttaa aaaaatccgc atgaaattga
      421 cccgcgaatc agtacaacgg gtggcaactc tggcgcgctt gcgtctgacc gcagatgaag
      481 aaaccgcact cgctggcgag ctcgatcgta tcctcggtta catggacaaa ctcaacgacc
      541 tcgacacttc aaacgttcca gttttcgcac attcaaacga tgtgagtgaa atattggaga
      601 agcttcgcga tgacaaagtc gtgaaccggc ccaataccga catcctgctg gcgaacgcgc
      661 cggaacgcga caaaacattt ttccgagttc cgaagatcat cgaatgagct tgagtgcgct
      721 tacacttcaa caagctagcg agaagctgcg ccggcgtgag atctcatctg tcgagcttac
      781 cgaggcggtg tttcaacgca tttcggcagc cgatgataag atccacgcct atcttacctt
      841 ggcccgtgaa agcgctttgg agcaagcgaa gcaagccgac gcacggcgga atagcgcaac
      901 acaactttcg ccgcttttgg gtataccaat cgccgtaaaa gataattttc tcacacgcgg
      961 cgtgcgcacc acttgcgcgt ccaagattct cggcgacttc atgccgcctt acgatgccac
     1021 cgccatcgtt aaactgcacg ccgctggtgc cgtcatcgtc ggcaagacca atcttgacga
     1081 gttcgccatg gggtcctctg cggagaattc ggcatttcac ccgacgcgca atccgtggaa
     1141 tctcgggcgc attcccggtg gctcttccgg cggttcggcg gctgctgtcg ccgccgacga
     1201 gtgcctcgcg gcgctcggca ccgacactgg cgggtcgata cgccaacctg cggcttgctg
     1261 cggtattgtc ggcctcaagc cgacctacgg ccgtgtcagc cgctacggta tcatcgcctt
     1321 cgcctcatcc atggaccagg ttggtcccat gactaaagac gtcagcgatg cggcatgcct
     1381 ccttcaagtc cttgcgggtc acgatccagc ggattcgaca tcatcaattc gttccgttcc
     1441 cgactatagc gctgctttga atggcgacgt aaaaggtatt tgtatcggcg tgccgaaaga
     1501 atacttcgtc agcggcattc aacctgaggt tgagaaatcg gtccgcgcgg cggttcagga
     1561 actggcaaaa aacggagcga gaatcgaaga aatctcgctg ccgcacaccg aatacgccgt
     1621 ggcagtctat tacgtcgttg ccactgcgga ggcaagttcc aatctcgcac gctacgacgg
     1681 catgcgcttc ggccatcgtg ccgaagccaa agatctcacc gaaacttaca tgattacccg
     1741 agcggaaggc ttcggcgcag aagtgaagcg gcgcatcatg ctcggaacct acgtgctgtc
     1801 gcacggctat tacgacgctt actatctaaa agcccagcga gtgcgcacgc tcatcaggag
     1861 agattttgac aatgccttca cacactgcga cgtcatagtc acaccaacgg caccgacgac
     1921 ggccttcaaa ctcgccgaga agactcaaga tccgctgcaa atgtacttgt ccgacatcta
     1981 cacgatctcg accaatttag ccggcctgcc tgcgctctcg ctcccctgcg gctttgataa
     2041 cgacaacctg ccaatcggtt tgcagatgat cggtaaacat tttgacgaag ccaccctcct
     2101 gcgcgccgcg catgcgtacg agcaagctac ggattggcac aaaaaaacac cgacactgta
     2161 atggacgatg taaatttatt aatagataac ccacgagctt ctcttgctca ctttccaagt
     2221 tttttaatga tccatcctcc attattcatt gtcatttcac tgcttcggag cgatcgccag
     2281 tgaaatacga acccgtcatc ggtctggagg tccacgccca gctcctcacc gcatcaaaga
     2341 tcttttgtgg atgctctacg caattcggcg gtgaacccaa cgcccatacc tgtcctgttt
     2401 gctgcggctt tcccggcgtc ttgccggtac tgaataaaaa agtagtcgag ttcgccatca
     2461 aagcggggct ggcgactcac tgcgaaatta cccgggcgag tattctcgcg cggaaaaatt
     2521 atttctatcc agacttgccc aaaggttatc agattagcca gtacgaatta ccgatctgtt
     2581 gcgacgggta catcgaattt actctgaacg atacgcttaa acgggtgcgc ctgacgcgca
     2641 ttcacatgga agaggatgcc ggtaaaaata tccacgatgc ccatggcgat tcgagcctga
     2701 tcgatttaaa tcgcgccgga gtgccgctac tggaaatcgt cagcgaaccc gacatgcgga
     2761 ctaccgagga ggccggtgct tacctgcgcg cgctgcgcgc gatcttgcag tttcttgaag
     2821 tatgcgacgg caatatggaa gaaggcagtt ttcggtgcga tgccaacatt tcagttcgtc
     2881 ccgcgggcac ggaaactttg ggcacgaaaa tcgaaatcaa aaacttgaac tcatttcgcg
     2941 cggtcgagcg ggcgctggaa tttgagattg accggcagag cgacactctg aacgaaggcg
     3001 gaaccttggc acaagaaact cgtctatggg acgagcatcg cgaggagact cgttccatgc
     3061 gttcaaaaga gtcggcgcat gactaccgct atttcccgga tcctgatctg ttaccactcg
     3121 tgattcccga aagctggatc gaggagatcc gcgccaagct gccggaattg ccggctgccc
     3181 gcaagcgacg cttcataacc gaccattgcc tgccggctta cgacgccgaa ctgctcacca
     3241 gccgtaagga tatcgccgac tatttcgaaa gcgcggtgcg ggtttacagc aacgccaagt
     3301 cattgagcaa ttggatcgtc ggcgatcttt tccgggtact gaaagagcgc aaactggacg
     3361 agcaactcta catcgacacg tggccgattc aggcgccgca tttggcggaa ttggtgcagc
     3421 taatcgatcg gggcaggatc aacggcaaaa ttgccaaaat ggtcttcgaa tcgatgctga
     3481 atagcgatga caaaacgcca cagcagctag ttaatgaaaa agggcttgaa caagtatctg
     3541 acgcaggcag tatcgaagcg gcgatcgacc aagtgctgac tagtaatccc aaacaagtgg
     3601 aacagtaccg ctccggtaat gacaaggtat ttggctttat cgttgggcag gtcatgaaag
     3661 cgacccaagg caaagccaac ccgcaaaagg ttaacgaaat tctccgcgac aaactaaacc
     3721 ggtagcgtag tttgattgct acggcttcgc ggccaattac gacgaatgtt tcaaggaatc
     3781 gacgtccgcg cttccggccg aaacgcttgt ggtaaattta aaatgattcc agccagtcca
     3841 tcttgcttta tagcttttag ttgacgctca gcatcgtcgc gcaggacgaa gttgccgagc
     3901 ctaacgcgca aggcgccgtc gttgccggct tcggtgacgg agacggcgaa accttttttc
     3961 tccattccct cagcccagcg ttgcgcgcgc tccttgtcat tgaacgctag ttgaatgatg
     4021 aatccctcta gcggcttcgg ggatgccggg cgggcgacta ccgccttggc tggcgcggct
     4081 tcgatcggtt tattcgggag tgacgccaac tgttcgggta tagggtttac cgaaggcttc
     4141 tcaattccta cctgtgaggc gggaacaaaa gggcgcgtag gcgcttcgat cgccgccggc
     4201 cggctcgaaa ttaagggctg gccggacacc aacagaggcg gcgcaactgg cacctcggtt
     4261 agcgccgatt gaaagaacgc cggcggcgcg ctggcaatct caatcggctt agcttcggcc
     4321 ttcccgttcg gcgtcggcgg cacagtaatg ggtgacctca ctgaagttcc gacgccgagt
     4381 ggctcagttt cgccgggctg aataggcgcg gccacgggct cctgtctcaa gggctccggt
     4441 gtcactgatg ctgcagcggg aggcgcgcta agtattttcg cgacactggg ttccggtttg
     4501 gacctagtcg tactcgcggc tttttccacc ttaggtgatt caagaccggg cacatttgcc
     4561 gcaactagag actctgtcgg cagataagtg ttcggctcga ggggcgacag cttcggcgtg
     4621 tcgacaaggc gcgctggtgc tgcgctttca ttcggccgcg ctaaattgac ctgcgccgct
     4681 tctttttttt ctggctctgg cgcaccaacc gcaggctgtc ccaattccac tggtgctggc
     4741 ggcggcaact tcggctgggt taccgatcca ggggcacgca tcacgtttgt cgttggctcg
     4801 gcaattttac tgatgggctt tataattgga gtagcttctt gagtctcagg aattcgagaa
     4861 gagactg
//
LOCUS       DATLCH010000032         6231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9747015, whole genome shotgun sequence.
ACCESSION   DATLCH010000032 DATLCH010000000
VERSION     DATLCH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9747015"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1096)
                     /locus_tag="VMZ02_00870"
     CDS             complement(53..1096)
                     /locus_tag="VMZ02_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUR70504.1"
                     /translation="MKAIVIREFGAPEVMHLEDVGEPKAPPGEIVIKVHAVSVNRTLD
                     CVVRAGKYPVTIQMPHVLGVDPAGAVVKVGSGVSRFKIGDRVATISLIRCGKCKQCAH
                     GVEANCLASRHIGLHRWGGYAEYVSVPERNAFHIADNLTYAEGTVITRHYPMAFNLLT
                     GKTQVRAGEWVLVMGATGALGSCCVQVAKMFGARVIAAAGADARVAIAINNGADFGIN
                     YRTQDLAKKVMALTDGDGVDIVCESIADPSLWPGAFNSLAIGGRMVTAGAHGGGNVML
                     DVKRLYLRRISILGAAGTNPADVDRALEAARAGRIKAIPHRVMPLSEAAEAHRIVENN
                     QIIGKIVLEPGND"
     gene            complement(1236..2216)
                     /locus_tag="VMZ02_00875"
     CDS             complement(1236..2216)
                     /locus_tag="VMZ02_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70505.1"
                     /translation="MLKRLLPIVLIALLGVAATAAAQTKRIAVAYSAVSATQSSFFVT
                     KDAGIFEKHGLSVDPVYVASGTKVAQAVIAGEFPVALAGGTVVNATLAGGDIVIIGGL
                     VNVPSFYVYVHSSIKRPEDLKGKAIGITTYGSSTDFSIRYLIKKWGMEPERDIKVLQM
                     GGQPQIVAAMVGGSVQGGVLSSPANYRAGKAGFQLLTDFKKVGLDYPTVSLVSTRSFI
                     KKDPQTVRRFMMAYSEGVERLYRDKEFTMKVIGKYTKTDDREALDSAYGFATTFIERP
                     PHLPYKAVETILTQLAEKDPKAREAKAEEFIDPTFYNEMEKSGYFKSIAR"
     gene            2361..3560
                     /locus_tag="VMZ02_00880"
     CDS             2361..3560
                     /locus_tag="VMZ02_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70506.1"
                     /translation="MKPEITAIQAKSECLNMRVIGHHDLNGHGDGMQLVKHGRYVYLA
                     HLGTSPMALSILDVADPTEPRLMAQMPHAANTHAHKVQVVGDILIQNQERPYFDKSVP
                     ANVPNEAGIKIYSIENPTQPREIGYLKLPGKGVHRMWFSDGKYAHVGAYLPGIEERAY
                     LIADISNPTTPREAGRWWIPGTKEGEATPADWTPFAGEHFYVHGAIPYGDRAYVALVD
                     AGMAILDISEISTPKAISHIDWSPPFGGYAHTTLPLPGRKLVVAVDESVKYDCQEGEK
                     RVWIIDIREEKNPVIVSTCPVPQGDFCSRGLRFGPHNVHENRPGSFQSENIIFVTYYN
                     AGLRVFDIRNQYRPEEIGYFIPPAPEGQKAPMFNDLYVDTDGLIYVTDRIKGGLYVIE
                     YTGANCD"
     gene            3767..5824
                     /locus_tag="VMZ02_00885"
     CDS             3767..5824
                     /locus_tag="VMZ02_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HUR70507.1"
                     /translation="MTTFTAKPVHHLLRAKSVAIIGASQKGAWPQGIYRNLKGAKFPG
                     GVYLINPNYQELYGDKCYPSLAACPEVPDELLILIPTRAVLGVLEEAAKLGTRSATIY
                     TAGFGEGDDEKGKERAQQMRELCDRTGMVICGPNCMGSYSVGEGLWTFPTATPLLKKG
                     PVGLIFQSGGSLGNWIKGATERGISFSYAISSGNEVSLDLVDYLSFLIDDPDTKVITL
                     MVEGIRRPNEFMAEAERALKIGKPILAVKLGRSEMGKRQAISHTGSLAGADEVFDAVC
                     KRLGIIRCPTLEDMTEMTLAFMLGRFPKGSRAAIVVNSGGMKGLICDHCDDLNTNLAQ
                     LSDKTKESVRPLIPPELAVENPLECGVAGFGDEAGFINIVKLHAEDPGVDLMAIHGEL
                     PRGQTRNAGLFKSVLEATEKPVLAFGRSTYSCTDESRAFQEEAGMPFLQAIKPTLRAL
                     AGLGMYGERRIAGVPTLPAANGNPAVLNGEMLDKLLQSKGIGLPRQALADSAADAAAR
                     AKELGFPVALKLIAADVVHKTEAGAVVLGLKNAEEVQTEGQKLLAKTSGKAQLLVQEM
                     VQGTEVLVGARTDPQYGPFLMVGLGGIFVEVLKDVSIRLLPVDEQEARAMLKELRGYK
                     ILEGVRGQARRDVDALVRAMVGLSELFAAHRNSLSDIEINPIMVRAEGGGIAAVDVRL
                     VRK"
     gene            complement(5912..>6231)
                     /locus_tag="VMZ02_00890"
     CDS             complement(5912..>6231)
                     /locus_tag="VMZ02_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70508.1"
                     /translation="ERRDTMLRFGRATVESIHFFKNNKAQTLTILKKYAKTDSTTLDS
                     AYSYIRGAVPDLPYPTLEGMKTILTEMGRSRPEVLKYDAASMVDPSLIKTIDDEGFLK
                     KLK"
BASE COUNT         1348 a         1647 c         1837 g         1399 t
ORIGIN      
        1 gaaatgacaa caattcgaaa cttactgtct tggcgttttt gcgggaataa tcttaatcat
       61 tacctggttc gagaacgatc ttgccaatga tctgattgtt ctcgacgatg cggtgcgctt
      121 cggccgcttc gctcagcggc atcacgcgat gggggattgc tttgattctt ccagcgcggg
      181 cggcttccaa cgctctatcg acatccgccg gattggtgcc ggcggcaccg aggatgctga
      241 tacggcgcag atagaggcgc ttgacgtcga gcatgacatt gccaccgccg tgggcgccgg
      301 cggtgaccat gcggccgccg atggccaggc tgttgaatgc gccgggccat agcgacggat
      361 cggcgatgct ttcacagacg atatcgacgc cgtcgccgtc agtgagtgcc ataacttttt
      421 ttgcgagatc ttgagtgcga tagttgatgc cgaagtcggc gccgttgttg attgcaatcg
      481 cgactctcgc atccgcgccg gcggccgcaa tcactcgggc gccgaacatt tttgccacct
      541 ggacgcagca gctgcccagc gccccggtgg cgcccatcac caacacccac tcgccggcac
      601 gtacctgggt tttgccggtg agcaaattaa acgccatggg atagtggcgg gtgattaccg
      661 tgccttcggc gtaagttaaa ttgtcggcga tgtgaaaagc gttgcgctcg gggacggaaa
      721 catattcggc gtaaccgccc cagcggtgca ggccgatatg cctgctcgcc agacaattgg
      781 cttcaacgcc atgcgcgcat tgtttgcatt tgccgcagcg gataagcgaa atcgtcgcga
      841 cccgatcgcc gatcttgaat cggctgacgc cgctgccgac ttttacgact gcgccggccg
      901 gatcgacccc gagcacatga ggcatctgga tggtcaccgg atatttgccc gcgcgcacta
      961 cgcaatcaag ggtgcggttg accgacaccg catggacttt gatgacgatc tcgccgggtg
     1021 gagcttttgg ttcgccgaca tcttcgagat gcatgacctc gggcgcgccg aactctctga
     1081 tgacgatggc tttcatagtg atgtacagct ggtgggttag gaacgaagtt tttaccgcga
     1141 aggcacgaag cgcacgaagt ttggaatact taaggccaaa acctgcgcgg cctgcgcgcg
     1201 ttcgcagaaa aaacgattat tccaatcgta cagatttagc gggcaatgct tttgaagtat
     1261 ccagattttt ccatttcgtt gtagaacgtc gggtcgatga attcttcagc cttcgcttcg
     1321 cgcgctttcg ggtctttctc ggcgagttgc gtcaaaatcg tttcgaccgc tttgtagggc
     1381 aggtgcggcg gccgttcgat aaacgtcgtc gcaaagccgt aagctgaatc caaagcttcg
     1441 cggtcgtcgg tcttggtgta cttgccaatg accttcatag tgaattcctt atcgcggtag
     1501 agccgctcga cgccttcgct ataggccatc atgaagcggc gcacggtctg cgggtctttt
     1561 ttaataaacg accgggtcga taccaagcta acagtgggat agtcaaggcc gactttcttg
     1621 aaatccgtga gcagttgaaa tccagcttta cccgcccggt aattggcggg cgatgataac
     1681 acgccgccct ggacgctgcc gccgaccatg gccgcgacga tctgcggctg gccgcccatc
     1741 tgcaacactt tgatatcgcg ctccggctcc atgccccatt tcttgatcaa gtagcggatc
     1801 gaaaaatccg tcgatgagcc gtaggtagtg atgccgatgg ctttgccttt cagatcctcc
     1861 ggccgtttga tcgacgagtg aacgtagacg tagaacgacg gcacgttgac taggccgcca
     1921 atgatgacga tgtcaccgcc ggcgagcgtc gcattaacca ccgtgccgcc agccagcgcc
     1981 acaggaaatt caccggcgat gaccgcctgg gcgaccttcg ttccgctggc gacgtagaca
     2041 gggtcgacgg ataaaccatg tttttcgaag atgccggcat ctttggtgac gaagaacgag
     2101 gactgcgtgg cgctgactgc gctgtaggcg acagcgatcc gtttggtttg tgccgctgcc
     2161 gtggcagcga ctcctaacag cgcaatcaga acgatgggca gcagacgttt cagcataata
     2221 ggctcctgca ctcggttctc gagtttgctg gttctaacat tttcagtgca gcctagccac
     2281 tgagccgtgc cgcattgaca agcttccgag gccgacatta tatgatccga gttgttcaac
     2341 atttctgcac ggagacacgg atgaaacccg aaattacagc gatccaagcc aagagcgaat
     2401 gcttgaacat gcgagtgatt ggccatcatg acttgaacgg acatggtgat ggcatgcaac
     2461 tggtcaagca cgggcgctat gtttatttag ctcatctcgg cacgtcgcca atggcgttga
     2521 gcattctcga cgtcgccgat ccgaccgagc cgcgattaat ggctcagatg ccgcacgcgg
     2581 cgaacactca tgcgcataaa gtgcaggtcg tcggagatat tctgattcaa aatcaggaac
     2641 ggccctactt cgacaaatcg gttcccgcca acgtgcccaa cgaagccggc attaaaattt
     2701 attcgatcga gaacccgacc cagccgcgcg agatcggtta cttgaagctg cccgggaaag
     2761 gggtgcaccg catgtggttc agcgatggca aatacgccca cgtcggcgcg tatttgccgg
     2821 gcatcgagga gcgggcttac ttgattgcgg atatttcgaa tccgaccact cccagagaag
     2881 cgggacgctg gtggatcccc ggcaccaagg aaggtgaagc gacgcccgcg gattggacgc
     2941 catttgccgg cgagcatttc tatgttcatg gggcgattcc ttatggggac cgtgcctatg
     3001 tggccctggt cgatgccggt atggcgatcc tcgatatctc tgagatcagc acgccgaaag
     3061 cgattagtca tatcgactgg agcccgccct ttggcggcta cgcgcatacg acactgccgc
     3121 tgccgggacg gaagctcgtc gtcgcggtgg acgagtctgt gaagtatgat tgccaggaag
     3181 gcgagaagcg ggtgtggatc atcgacattc gtgaagaaaa aaatccggtg atcgtcagca
     3241 cttgcccggt accgcagggc gatttctgca gccgcggtct gcgcttcggt ccgcataacg
     3301 tgcacgagaa tcgcccgggt tcgttccaaa gtgagaatat tattttcgtg acgtactaca
     3361 acgcaggctt gcgcgtgttc gacatccgca atcaatacag gcctgaagaa atcggctact
     3421 ttatcccgcc ggcgcccgag ggacagaagg cgccgatgtt caacgatctt tatgtcgata
     3481 ccgacggtct catttacgtc acggaccgga tcaagggcgg gttgtatgtg attgaataca
     3541 ccggggcgaa ttgtgattga cggcacaatg gcaatctcgc cgagaaaatc cgcttctttc
     3601 gcagcaattg cacagtgggc atattcggaa tcggctggtt gagcttcgct cgccgaagaa
     3661 attttctaat cactattttc cgaatcattg cgattgcgct ccacttcctt gcagtcgatt
     3721 agtgtctatg acatagcaat cccatctcag attagaagga actttcatga ctacattcac
     3781 ggcgaagccg gtccatcatt tattgcgcgc gaaatcggtg gccatcatcg gcgcttcgca
     3841 aaagggcgct tggcctcagg gaatttatcg taatttgaag ggcgcaaaat ttcccggcgg
     3901 cgtttatctg atcaatccca attaccaaga gctttacggc gacaagtgtt atcccagcct
     3961 tgctgcatgc ccggaagttc ccgatgaatt gctgatcctg attcccaccc gtgccgtatt
     4021 gggcgtgctt gaagaagcgg ccaagctggg cacgcgttcg gcaaccattt acactgcggg
     4081 attcggcgag ggtgacgatg aaaagggtaa agaacgagcg cagcagatgc gcgagctgtg
     4141 cgaccggaca ggcatggtga tctgcggtcc caactgcatg ggctcttact cggtgggtga
     4201 aggattgtgg accttcccga ccgcgacacc gctgctcaag aaaggtccgg tgggtttgat
     4261 ctttcagagc ggcggttcgc taggtaattg gatcaaaggc gccaccgagc gcgggattag
     4321 cttctcctac gcgatttcca gcggcaatga agtgagtttg gatctggtcg attatctttc
     4381 attcttaatc gatgatccgg acaccaaagt gattacgttg atggttgaag gcattcgccg
     4441 gccaaacgag ttcatggcag aggcggagcg ggcgttaaaa atcggcaagc ctattcttgc
     4501 ggtcaagctc gggcgctcgg aaatgggcaa acgccaggcg atttcgcata ccggatccct
     4561 cgccggcgcc gacgaagtat tcgacgcggt gtgcaaacgc ttgggcatta tccgttgtcc
     4621 gactttggag gacatgacgg agatgacatt ggcttttatg ctcggcagat ttcctaaggg
     4681 cagccgggcc gcgattgtcg ttaactccgg cggtatgaaa gggctgattt gcgatcactg
     4741 cgacgacttg aataccaact tggcgcagct cagcgacaaa accaaagaat ccgtgcggcc
     4801 gctaattcca cccgaattgg ctgtcgaaaa tcctttagag tgcggcgtgg cgggtttcgg
     4861 tgatgaagcg ggtttcatca atatcgtcaa acttcacgcc gaagatccgg gcgtcgatct
     4921 catggccatc cacggcgagc tgccgcgcgg ccaaactcga aacgccggat tgttcaaaag
     4981 cgttctcgaa gccaccgaga agccggtgct ggcgtttggc cgctcgacgt acagctgcac
     5041 cgacgaaagc cgggcgtttc aggaagaagc gggaatgcct tttttgcagg cgatcaagcc
     5101 gacgctgcgc gcgctcgctg gcttgggaat gtacggtgag cgcagaattg ccggggtacc
     5161 gacgctgccc gcggcaaacg gaaatccggc ggttctcaat ggtgagatgt tagataagct
     5221 cttgcagagc aagggtatcg gcttgcccag gcaagcgctc gccgactccg cggccgacgc
     5281 agccgcgcgc gcgaaagagc tcgggttccc ggtggctcta aaattgatcg cggctgacgt
     5341 tgtgcacaag accgaagccg gtgcggtggt gctgggatta aagaacgctg aagaagttca
     5401 gacagaagga cagaaactct tggcgaagac ttcaggcaaa gcgcaattat tagtccaaga
     5461 aatggtgcag ggcactgaag tgctcgtggg cgcgcgcacc gatccgcaat acgggccgtt
     5521 tttgatggtc ggtctcggcg gcatcttcgt tgaggtattg aaggatgttt cgattcgctt
     5581 gctgccggtg gacgagcagg aagcgcgcgc gatgttaaaa gagcttcgcg gctataagat
     5641 tctcgaaggt gtgcgcggtc aagcccggcg cgacgttgat gcgctggtaa gggcgatggt
     5701 cgggctatcg gaactcttcg cggcgcaccg aaattcattg tcggatatag aaatcaatcc
     5761 gatcatggtg cgcgccgagg gtggcggtat cgcggcggtg gatgtgcggt tggtgcggaa
     5821 atagagaaaa tagccgctaa gaaccctcga gcacgcatgt aaggggtggg gtcttccatc
     5881 cgtgttgcgg aacacgaata attgcgcctt cttatttcag ctttttcaaa aaaccttcgt
     5941 cgtcaatggt tttgatcagg ctcggatcga ccatcgacgc ggcgtcgtat ttcaacacct
     6001 ccggacggct ccgacccatt tcagtcagaa tcgttttcat tccttccaga gttggatagg
     6061 gcaaatcggg gacagcgcct ctaatatagc tataagcgct gtcgagcgtg gtgctatcgg
     6121 tcttggcgta tttctttaag atcgtgagag tctgcgcctt gttgtttttg aaaaaatgga
     6181 tcgactcgac ggtggcgcgg ccgaagcgca gcatggtgtc gcgtcgttct t
//
LOCUS       DATLCH010000033         4053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9053406, whole genome shotgun sequence.
ACCESSION   DATLCH010000033 DATLCH010000000
VERSION     DATLCH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9053406"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /locus_tag="VMZ02_00895"
     CDS             <1..1431
                     /locus_tag="VMZ02_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HUR70509.1"
                     /translation="KISNQSPTHTAVVYKSERITYAELEKTVQVLKDDLRRIGIEPGH
                     KVGVLFPNGPEHVAATLAIVGIGAVIVFLSPLSSAGEIAALADEVSLDALCYAGRFDV
                     YLSSITGTASGELELFTGRELIMIRRRPGSTSSEERRRLVEINAAALRFSSGTTGKAK
                     GIINSHCTILERAKVHFQSPPLHQDDAVLWMQPLGRLPGPLAYLLRGATLVISDALET
                     ATLEQVIHQERVTHIYTVPMFFRAILNDEFDARGLDRVRYLLSGGTALGQQLAEQFSA
                     KFGHEIVEHYGLAETGTVFMNESQDPRKRGSIGVPVRSEVKLLSPTGEGLEGEATGEL
                     IVRCAGLFDAYYQPWSVRDEVLADGWFNTGDVARRDADGYYWIVGRVKEMINVAGIKV
                     FPQDLESILATHDAVEEAIVFAMPNKRFGEVPHAKVKLRAGASCSKKALLSYANDRLP
                     LFKSLRGLEFVESIPKTVTGKVKRLS"
     gene            1446..1688
                     /locus_tag="VMZ02_00900"
     CDS             1446..1688
                     /locus_tag="VMZ02_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70510.1"
                     /translation="MQKVDDTEKRLIAVLRRVAGPKVNLTRTNLGLESMKMLEVVIAV
                     ENEFEITFPEDAPLGKVTASLRNLKQYVKKLISETG"
     gene            complement(1707..2864)
                     /locus_tag="VMZ02_00905"
     CDS             complement(1707..2864)
                     /locus_tag="VMZ02_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70511.1"
                     /translation="MLQGYRIFDADAHGSINPSMWEDLPEQFRARRPRPVTIHDDAGL
                     GNFTAGWLIEGSVEPHALGPGAQQANTPRNVLVEFGANTSREHCSIGSMTLNDPRARL
                     ADMDRLGFDHQMIFPSTLYAHMTTDPEFEAALYRAYNRFVAKQCAAHSQRLKWSGLLP
                     LREPRQALLAIDEMQRLGARAAVVFGTAGERLLSDPSFLPIWDEFAKSRLPLCVHMGM
                     SYPPLAQLGRSIYDGHVIGMSLPAQLAFMAVVGHRMLDRYPELKVAFLEFGAEWIFYM
                     VSRMDHYLPLDRGAHPFGLSMPNVAELPRDNIRDYVKSGRLFIAAEADDRMLAQLFDL
                     IGEDHVLFSSDFPHGEGRDNAAQEILERQDLSATHKRKLLYDNTVGFFGAP"
     gene            complement(3195..>4053)
                     /locus_tag="VMZ02_00910"
     CDS             complement(3195..>4053)
                     /locus_tag="VMZ02_00910"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70512.1"
                     /translation="TFKKYGLDVRMVQLRNGALSMSTLASGQVQYNYGSPANALSAAA
                     GGMRLQCVASAVAKIPRELVARKEIRTLEELRGKTFGVQSIGGGFWLQTMIALDYLGI
                     DPDKYDLKMRILGDTATVTQALGSGGVDATVIPYSFSEAAKRAGANVLADVGKLPIPY
                     QGTTLCFLRDSPSHNPDIISRLLKGLVDAVVMIHDPSAKLDVTEILRKSFRFPRLEDA
                     EASYRVLGQMATIDLLPDPAAWKIVQRVVARINPKVSQVDIGQLNNPTYVRALEESGF
                     LPDARRRLR"
BASE COUNT          910 a         1087 c         1189 g          867 t
ORIGIN      
        1 aagatatcca atcaatcgcc gacgcacact gccgtcgtct acaagagtga gcgaattacc
       61 tacgcggagc tcgagaaaac cgttcaggtt ttgaaagacg accttcggcg tatcggcatt
      121 gaaccgggcc acaaggttgg ggtgttgttt cccaacggtc cggaacatgt tgccgcgacc
      181 ttggcgatcg ttggcatcgg cgcggtgatc gtatttctct caccgttgtc gagcgctggt
      241 gagattgccg cgctggccga cgaagtgagc ttagatgcgc tttgctacgc cgggcgtttc
      301 gacgtttatc tctcgagtat taccggcacg gcttcaggcg agctggaatt gttcacaggc
      361 cgggagctta tcatgattcg acgacggccg ggatcaactt ccagtgaaga gcgccgccgc
      421 ttggtcgaaa tcaatgccgc ggctcttcgc ttcagctcag ggacgacagg aaaagccaag
      481 ggcatcatca attctcattg cacgatattg gagcgcgcca aggtccattt ccagtcgccg
      541 ccgttacacc aggacgatgc ggtgttatgg atgcagcctc tcggtagatt gccgggaccg
      601 ctcgcctatc tgttgcgcgg ggcgacttta gtgatcagtg acgcgttgga gactgcgact
      661 ttggagcaag tgattcacca ggagcgagtc acgcatattt ataccgtgcc aatgtttttt
      721 cgcgcgatct taaacgatga gtttgacgcc cgcggccttg atcgggtgcg ctacctttta
      781 tccggcggga cggccctggg acagcagttg gcagaacagt tctcggcaaa attcggtcat
      841 gaaattgtcg aacactacgg gctggcggag actggcacgg tgttcatgaa cgaaagccag
      901 gatccaagaa aacgcggctc aatcggcgtc ccggtgcgga gcgaggtgaa actgctatct
      961 cccacaggag agggtctcga aggcgaagct accggcgaac ttatcgtccg ctgcgccggg
     1021 ctgttcgatg cctattatca gccctggagc gttcgcgacg aagttctcgc cgacggctgg
     1081 ttcaatacgg gagatgtcgc tcggcgcgac gctgacggtt attattggat cgtcggcaga
     1141 gtcaaggaga tgattaacgt cgccggaatt aaggtgtttc ctcaggatct cgaaagtatt
     1201 ctcgctaccc acgatgccgt tgaggaagcc attgtttttg ccatgccgaa caaacgcttt
     1261 ggcgaagtgc cgcatgccaa ggtcaaattg cgcgcaggtg ccagttgtag caaaaaggcg
     1321 ctcttgagtt acgccaatga tcggctgccg ctgttcaaat cgttgcgcgg tttggagttt
     1381 gtcgagtcga tccccaaaac ggtcaccggc aaagttaaac ggctaagtta acaataggcg
     1441 acgagatgca gaaggtcgac gataccgaaa aaagactgat cgcggttctg cgccgtgtgg
     1501 ccgggcccaa agtcaatttg actcgaacca atctcggtct ggaatcgatg aagatgcttg
     1561 aggtggtcat tgccgtggaa aatgaattcg aaattacttt tccagaagac gcgccgctcg
     1621 gcaaagtaac cgcctcgctc cgtaacctta aacagtatgt caagaaactg atcagcgaaa
     1681 ccggctaatc acgccaacat ttattttcac ggcgcgccga aaaagcctac cgtgttgtca
     1741 taaagaagct tgcgtttgtg cgtggccgag aggtcctggc gctcgaggat ttcctgggcg
     1801 gcattgtcgc gcccttcacc gtgcgggaag tcggaggaga acagcacgtg atcctcgccg
     1861 atcaaatcga aaagctgcgc taacatgcgg tcatcggctt cggcggctat gaagagacgg
     1921 ccggacttaa cgtagtcgcg gatgttgtcg cgcggcaact cggcgacgtt gggcatgctc
     1981 aagccgaacg gatgggcgcc gcggtcgagc ggcaagtaat gatccattcg cgaaaccatg
     2041 taaaaaatcc attccgcgcc gaattccaaa aacgccactt tcaactctgg gtagcggtcg
     2101 agcatgcgat ggccgacgac agccataaag gccaattgcg caggcaagga catgccgatg
     2161 acatggccgt cgtaaatgct ccgacccagt tgggccaaag gcggatagct catgcccata
     2221 tgcacgcaca gcggcagtct gctttttgcg aactcatccc aaatcggtaa aaagctcgga
     2281 tcggaaagca agcgttcgcc ggccgtgcca aataccacgg cggcgcgggc acccaagcgc
     2341 tgcatctcgt cgatagccaa tagcgcctgg cgcggctcgc gcaacggcag caagcccgac
     2401 catttgagac gttgagaatg cgcggcgcac tgcttggcga cgaagcggtt atatgcgcgg
     2461 tacagcgccg cttcgaactc cggatcagtg gtcatatgag catagagcgt gctcgggaat
     2521 atcatttggt gatcgaaacc cagacgatcc atgtccgcca accgcgcgcg cggatcgttc
     2581 agagtcatgc tgccgattga gcaatgctcg cggctggtat tggcgccgaa ttccactagc
     2641 acgttgcgcg gcgtgttggc ttgttgagcg ccggggccca gggcatgcgg ttcgactgat
     2701 ccttcgatca gccagccggc agtgaaattt cctaatccgg catcgtcgtg gatcgttacc
     2761 ggccgcggcc gccgcgcgcg aaactgttcg ggtaagtctt cccacatgct cgggttgatc
     2821 gaaccatgag catcggcgtc aaagatgcgg tagccttgaa gcataagcga tttcctctct
     2881 tgatgaatcg ttgatggtgg cgtgatgtgc agcgagattt gtcagcacga agtgttcatg
     2941 agcatacgct cgtaacaaaa tataaaattg tagcttccat atgcatcatc ctttcgatcg
     3001 tcatgccggc gtaggctggc atccagggag cgaaggatgg ataccggctt ccgccggtat
     3061 gacagaaacc ttcttgccgc tcatcgctgc gattgtacct aagcatgcat ttaagcagcg
     3121 ggcacgaaag acacaaaggc tttgcaaagc attatgctcg aacgcttggc ttagtggtga
     3181 atggtcggtg acagtcatcg cagtctgcgc cgggcgtcgg gcagaaagcc gctctcttca
     3241 agagcgcgga cataggttgg attattcagt tggccgatat ccacctggct aactttgggg
     3301 ttgatgcgcg cgacaacgcg ctggacgatt ttccaagccg cgggatcggg caagagatcg
     3361 atggtcgcca tttgtccgag cactctgtac gatgcttcgg catcttcaag ccgcggaaag
     3421 cgaaaactct tgcgcagaat ctcggtcaca tcgagcttgg cactgggatc gtgaatcatt
     3481 accacggcat cgaccaagcc cttgagtaag cgcgatataa tgtctggatt gtgcgacggc
     3541 gagtcgcgca aaaagcacag agtcgtgcct tggtagggaa tcggtaattt gccgacgtcg
     3601 gccaagacat tggcgccggc ccgcttggcc gcttcgctga agctgtaggg aatcaccgtt
     3661 gcgtcgacac cgccggaacc gagcgcttgg gtgacggtcg cggtgtcgcc gagaatccgc
     3721 atcttgagat cgtacttgtc gggatcgatg ccgagataat ccaaggcgat catggtttgc
     3781 aaccagaaac cgccgccgat gctttgcacc ccaaaggttt taccgcgcag ctcttccagc
     3841 gtgcgaattt cttttcgcgc cactaactca cgaggaatct tggccacagc agaagccacg
     3901 cactggaggc gcatgccgcc agcggcggcg ctcagcgcat tggccggcga accatagtta
     3961 tactgcacct ggccggacgc cagagtcgac atgctgaggg cgccattgcg cagctggacc
     4021 atacgaacat cgagaccata tttcttgaac gtt
//
LOCUS       DATLCH010000034         4293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5335679, whole genome shotgun sequence.
ACCESSION   DATLCH010000034 DATLCH010000000
VERSION     DATLCH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5335679"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="VMZ02_00915"
     CDS             complement(<1..1249)
                     /locus_tag="VMZ02_00915"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR70513.1"
                     /translation="MTNRFEVPPEQLAWRCDLTFLPFTCTADMTPLEDFIGQDRAIRA
                     IEFGLGVNKPGFNIFVTGLTGTGKTSIIKAFLKKLTSTTVAAAATETPDDWCYVYNFA
                     DPDRPQALKIRRGLGKALKSDMEQLIQNLQRDAKKMFESDDFARQRQAPIEQLQKKQQ
                     EMMEGLMEQAGRAGFTLRMTPSGIMLLPTKDGKPMQEADYMALTAADKKQLEERRSEI
                     EKHVEDTLREGKKVEREIAERLETVETEAADYLVRHPLTDLKQKYQDYPRVLAYLDGV
                     REHILKNLQRFKGESAPAGLIAGMSFAEPPGDPFLPYRVNVFVDNSDTQGPPIIVETN
                     PTYHNLFGVIEKKPIMGGYATDFTLIKAGSISKANGGYLVLYDREVLANAGCWEALQR
                     VIKNRELRIEEPGAFFGFVPPQGL"
     gene            1478..2800
                     /locus_tag="VMZ02_00920"
     CDS             1478..2800
                     /locus_tag="VMZ02_00920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HUR70514.1"
                     /translation="MLPSVTRPAEDEEVRISREFRREAKKHFKFSNQPEVERYVDRIA
                     RRILSATGPLSYEYRFFVVEEDQLNAFAVPGGSIYLFTGLIERAKSTDELAGVLGHEI
                     VHIKGHHMARSSGVDAVSVLSLLATVLLARSGSSGGQAAGIVGQAVAATRQAAYSRQL
                     EMESDTLGTRYMAAAGFDPRGTLAFLKTMDQERQLNPIDVPAYILSHPLTQERVANAE
                     LVVKSLAPNKPRAEDNETLKKVQIVIRMERSNRESVVTEYQRLVQKDPDNPELLHLLG
                     FAQLQLGSLGPAQKNYEKSWRLRPDHVGLLREIGRLSSQTGDYESAHKAFNRAIMLEP
                     SEPLNYFYFGEFLEKTGDFRAAAGAYLNAQNLAPLWDKPVFRLGQVYGKLDRLADGYY
                     YLGRAQLLQDEDERAIADFERALKIVGEGSPRGQVIKEELKTLRDRKR"
     gene            complement(2803..3183)
                     /locus_tag="VMZ02_00925"
     CDS             complement(2803..3183)
                     /locus_tag="VMZ02_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70515.1"
                     /translation="MYDDFLARILEPDIFLPSQFYGNGGLSRKLEGEKRLMIAILKDA
                     VECLEKSRASRSSAARISYQSAIDWIEDNDTDWLYSFNNICDLLGFDPAYLRAQLLKR
                     ENRYFKQTPIRNVSLASMNESASL"
     gene            complement(3381..4091)
                     /locus_tag="VMZ02_00930"
     CDS             complement(3381..4091)
                     /locus_tag="VMZ02_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR70516.1"
                     /translation="MAELTELKKKLAMSSRMLFNSGLVDYSGHISVRIPGSDHLLILP
                     HPVSRAVVKPDDMVVTDFDGTVIEGKYKAPSEVYMHARAYKARSDIQSVAHLHNHMVA
                     TLSMVDKPFYPASSNPGAFFGPGALPKYMDPALIHTIEQGDAVAKSLGNGDAVMLRGH
                     GSMVVAQSIEWVFAACVDLEEAAARFYQASLLGAVLVYNEDETQRVMKGRRKDSVVQK
                     VWDHNVAKTKLAGLMDGI"
BASE COUNT          989 a         1195 c         1119 g          990 t
ORIGIN      
        1 gcagaccttg gggcggtacg aagccaaaga aggcaccggg ttcttcgatg cgcaattcgc
       61 gatttttgat gacccgttgg agcgcttccc agcacccggc attggcgaga acttcgcggt
      121 catagagcac caaataaccg ccgttggctt tgctgatgga accagctttg atcagcgtaa
      181 agtcagtagc gtaaccgccc atgatcggtt ttttctcgat gactccgaac aggttatgat
      241 aggtgggatt ggtctcgacg atgatcggcg gcccctgggt atcgctgttg tcaacaaaca
      301 cgttgacccg ataaggcaga aacggatccc ccggcggttc ggcgaaggac attcccgcta
      361 tgagacctgc cggcgccgac tcgcctttga agcgctgcag gttttttaat atatgttcgc
      421 gcacgccgtc gagataggcc aataccctcg gatagtcctg atacttttgt tttagatcgg
      481 tgagtgggtg acgcacgaga taatcggcag cttcagtttc gacggtctcc agccgttcag
      541 cgatctcacg ctcgactttt ttaccctcgc gcagagtatc ttccacatgc ttctcgatct
      601 cactgcggcg ctcttccagc tgctttttgt ccgcggcagt aagcgccata tagtccgctt
      661 cctgcatcgg cttgccgtcc ttcgtcggca gtaacatgat gcccgacggc gtcatgcgca
      721 aggtaaagcc ggcgcggcct gcctgttcca tgaggccttc catcatttcc tgctgtttct
      781 tttgcagttg ctcgatcggc gcttggcgct ggcgcgcaaa atcgtcgctc tcgaacattt
      841 ttttcgcgtc gcgctgcaga ttctgaatca actgctccat gtcgctcttg agcgccttgc
      901 cgagaccgcg gcgaatcttt agtgcttgag gacggtcggg atcggcaaag ttatagacat
      961 agcaccagtc atcgggcgtt tctgtagcgg ccgctgctac cgtcgtgctg gtcagttttt
     1021 taagaaatgc tttgatgatg ctggtcttgc ccgtgcccgt caggccggtg acaaaaatat
     1081 taaacccggg cttgttgacg cccaagccaa actcgatggc ccgaatagcg cgatcctgcc
     1141 cgatgaaatc ttccaatggg gtcatgtcgg cggtgcaagt aaaaggcaaa aatgttaaat
     1201 cacaacgcca cgcgagttgt tcaggaggta cttcgaagcg gttagtcatg gcagctcctc
     1261 tgctttgatt tcaaagaatc gagaataggg acttgcccag gggtcgtcaa ccacggcgag
     1321 tgatgaaatg acaaacgacg tcagtattga acccatgcgg gctgctctct atactccgtc
     1381 aatgataacg ccgggccgag ctcatcggtg gccagcatgc tgcgtaacgt tttttttgat
     1441 cctttcactt tgcgccggtt gccgcacgat agccccgatg ctgccatccg ttacccggcc
     1501 agccgaggac gaagaagtta gaatcagccg cgagtttcgt cgcgaagcca aaaaacattt
     1561 taagtttagc aaccaaccgg aagtcgagcg ttacgtcgat cgcattgccc ggcgtattct
     1621 cagcgccaca ggtccgctct catacgaata tcgatttttt gttgttgaag aagatcaatt
     1681 aaacgccttt gccgtcccag gcggatcgat ttatctcttc accggactga tagagcgggc
     1741 gaaatccact gacgaactgg caggtgtgct tggccacgag atcgttcata tcaaagggca
     1801 ccacatggca cgctcctcgg gagtcgatgc cgtatcggtc ttgagcttac tcgccaccgt
     1861 tctgttggcg cgcagtggct cctcgggcgg ccaagctgcg ggcatcgtcg gtcaagcggt
     1921 cgccgcgacc cgtcaagcag catacagccg gcaattggaa atggaatcgg acaccttggg
     1981 cacgcgctat atggctgctg cgggcttcga tccgcgcggc acgttggcat ttttgaagac
     2041 gatggaccaa gaacggcagc tcaatccgat tgacgttcca gcctatattc tcagccatcc
     2101 actcactcag gaacgtgtcg ccaatgccga gctagtggtc aaatccctag ctcccaataa
     2161 gccgcgagca gaagataacg aaaccctcaa gaaagttcag atcgtgatcc ggatggaacg
     2221 ttccaaccgc gaatcagtcg ttaccgagta ccagcggttg gtacaaaaag atccggataa
     2281 ccccgagctg ctgcacctgc ttggcttcgc tcagcttcag cttggctcgc ttggcccggc
     2341 gcagaaaaac tacgagaagt cgtggcggct ccgcccggat catgtcggtc tgctgcgcga
     2401 aatcggccgt ttatccagtc aaaccggcga ttacgagtcg gcacacaagg cctttaaccg
     2461 cgccattatg ctcgagccga gcgaaccgct aaattatttc tactttggtg aatttctcga
     2521 aaagactggc gacttccgtg ccgccgccgg tgcctatcta aacgctcaaa acctcgcgcc
     2581 tttgtgggac aaaccagtgt ttcgtctcgg tcaggtctac ggcaagctcg accggctcgc
     2641 agacggctac tactacctcg gtcgcgcgca actgctgcag gacgaggacg agcgcgccat
     2701 cgccgacttt gaacgagcgc tcaaaatcgt cggcgaaggc tcgccgcgcg gacaagtaat
     2761 taaagaagag ttgaaaacgc tgagagatcg taaacgctga gcttacaggc tcgcgctctc
     2821 gttcatcgac gccaaggaga catttctaat cggcgtctgt ttgaaatagc ggttttcgcg
     2881 cttgagcagc tgcgcccgca agtaagcggg atcgaaacct aacaaatcgc agatattgtt
     2941 aaacgaatac aaccagtcgg tatcgttgtc ttcgatccaa tcaatggcgc tctgatagct
     3001 gatcctggcg gcgctgctgc gcgaggcgcg agacttctct aagcactcca ccgcgtcctt
     3061 tagaatcgcg atcatcagcc gcttctcgcc ctcgagtttg cgcgataagc caccatttcc
     3121 gtagaactgc gaaggtagaa aaatatccgg ttctaaaatc cttgcgagaa aatcgtcgta
     3181 cattactgat cgaactcctc cataaatttg aattgcattc tataacacag ccaatatttg
     3241 cacacaagaa aaaacaccca atacagcgtc ttggaaacag aactggctta acatcaatcg
     3301 cagaaggcca agctcaaagc gcgcctcaat cgctggccaa agcccccaag gaggcaaact
     3361 atcgagccga acatctgcgc ttaaatgccg tccatcagtc cggcaagttt agtcttggcg
     3421 acattgtgat cccagacttt ttgcactacc gagtctttgc gacggccttt catgactcgc
     3481 tgggtctcgt cctcgttgta gaccaacacc gctcccagca gtgatgcctg gtagaaacgc
     3541 gctgccgcct cttcgagatc gacgcaagcg gcaaataccc attcgatgga ttgtgcaacc
     3601 accatcgaac catggccgcg caacataact gcatcgccgt tgccaagcga tttggcaacc
     3661 gcatcgccct gctcgatcgt gtgaatgagc gccgggtcca tgtacttggg caacgcgccg
     3721 ggcccgaaga aagcgccggg attggaagag gctggataaa atggtttgtc gaccatgctg
     3781 agagtcgcca ccatgtgatt gtgcaggtgc gccacactct ggatatcgct tcgcgctttg
     3841 taagcgcgcg catgcatata cacttccgac ggtgctttgt acttgccttc gatcactgtg
     3901 ccatcgaagt cagtgacgac catgtcgtca ggtttaacca ctgcccggct caccggatga
     3961 ggcaaaatca ataaatgatc gctgccagga atgcgcacgc tgatgtgacc gctgtaatcg
     4021 actagcccgg agttgaacag catgcgcgac gacatcgcga gctttttttt gagttcagtt
     4081 agttcagcca taaaataccc ctttcaaact attagcggaa aaattccggc catcatatac
     4141 actgaaatag ttcagtcgca acttgacttg ggccggtttg gccgtgaaat agacgtatca
     4201 ggattgaaag gatttgatca tggcaaacaa tagcgcggca gggatccgtt ggaatttgag
     4261 cgatcttttt gccgcacacg acgacccgcg aat
//
LOCUS       DATLCH010000035         9830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6767348, whole genome shotgun sequence.
ACCESSION   DATLCH010000035 DATLCH010000000
VERSION     DATLCH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6767348"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..416
                     /locus_tag="VMZ02_00935"
     CDS             18..416
                     /locus_tag="VMZ02_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HUR70517.1"
                     /translation="MATTIPESFKDLLNKVAFAHLATVMADGRPQVTPVWFSFDGTHF
                     WVNSAKGRLKDKNMRRNKYVALSIQDPDNPYRYLAIQGEVEEITENGADAHIDTLAKH
                     YLGKDKYPFRAPGEVRVIYKIRPQKISTMS"
     gene            complement(566..1516)
                     /locus_tag="VMZ02_00940"
     CDS             complement(566..1516)
                     /locus_tag="VMZ02_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HUR70518.1"
                     /translation="MLFLKNTPINQILSMSEMIETIEETLKEIACGRGYELPRRRIHH
                     PNGMIFGILPGSVHDAMGAYMQTDLNRKIHHENVMIFSTENGAPLMLFQDCSINEYRT
                     GAAGGIGAKYLARSDASRVAVLGSAVHAETQLKAVATVRKLTEGWVYSPTQERCASFA
                     AKMAQELEIPLHIAGSAEEAMANADVLIAATNSRTPVFDGKSLKDGMHITSIANGDKT
                     RTRQELDEVVLRRADVIFVTSKETVCVNESDIFRAVRDKVISWDKVHEISNLLLGKAV
                     GRSDDRQITLFKLQGTGIMDVAIASKAYQRLKNSGLAQSL"
     gene            complement(1607..2281)
                     /locus_tag="VMZ02_00945"
     CDS             complement(1607..2281)
                     /locus_tag="VMZ02_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HUR70519.1"
                     /translation="MDFRVISNNFAFLIAQGFFGIGAFTGGTLRLAVPAIILGSILGI
                     ILGLARLSHSPWLYGPAKVYVEFFRGVPLVMVIFWFWFIIPTLIGKSLPEYAVALTAF
                     VVFEAAYLAEIVRAGIQSVARGQVEAAIATGLSKKQLMRHVVLPQALRNMIPALVTQF
                     IVLLKDTSLASIIGYVDLTKAAQIVNNREIRPFELYLFIAIVYWLCSYAMSRYAGHLE
                     HQLASR"
     gene            complement(2283..3008)
                     /locus_tag="VMZ02_00950"
     CDS             complement(2283..3008)
                     /locus_tag="VMZ02_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HUR70520.1"
                     /translation="MTYHFDWSTLWTGQSGGWLLQGVITTLQIFAVGLIIAIVLGICA
                     GALRTVAIAPLRWLASSYVEFFRNVPLLVWMFFGYFSVPPLLPQAIQNWLFDHGAEFW
                     AAVFALGVYHGARFSEVMRAGIQSIPKTQLEAALSTGLTVGQAYRLIIIPVALRLIIP
                     PATNETVNLLKNSSVALTIGVAELTFQTRQIETYTAKAFEALAAGTVIYLALCLTIAS
                     IMARVERRVAIPGLIVQGHTGTS"
     gene            complement(3088..3945)
                     /locus_tag="VMZ02_00955"
     CDS             complement(3088..3945)
                     /locus_tag="VMZ02_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUR70521.1"
                     /translation="MKLSVAFLVAVVLATETTNLSFAESTLEKIDKSGTFVVGTRTGS
                     PPFAFVNKNNEWVGFSINLVEQGILPALSKKLNKQIKLEKKESTVPTRIPLLTSNAVD
                     LIAETMTDTQVRREQVDFSLTFFITGAQFLVRKGSPVKGIQSIAGKRVAAQQGSTNAK
                     ILREKVPNAKLVEFQDQPAAFQALSQRQVDAYTNDGIQLAGLRAKAPKPDDWTVVGDF
                     FSYDPYAMAMRKNDSDFRLLVNVGLMDAISSGLYFDLYEKWFGAKGEVPYPLTPENKR
                     FLQLQVVPK"
     gene            complement(3957..4685)
                     /locus_tag="VMZ02_00960"
     CDS             complement(3957..4685)
                     /locus_tag="VMZ02_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HUR70522.1"
                     /translation="MIEFHNVNKWFGALHVLSDIELKIAAGHVVVVCGPSGSGKSTMI
                     RCINRLETIQSGEIVVDGLSLSDPKLDAAKLRSNVGMVFQSFNLYPHMTVLQNITLAP
                     VKVRNITVTEAQRIAKELLDRVGIPDKAQVYPANLSGGQQQRVAIARALAMKPKVMLF
                     DEPTSALDPEMINEVLEVMTDLAKDGMTMVVVTHEMGFARRVAHRIVFMDQGKIIETA
                     EPEAFFAAPQSARAQQFLSKILSH"
     gene            4671..5324
                     /gene="sixA"
                     /locus_tag="VMZ02_00965"
     CDS             4671..5324
                     /gene="sixA"
                     /locus_tag="VMZ02_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HUR70523.1"
                     /translation="MKFDHGRCRLAERLGCKTLVGILYQQRLFARIIGAFADLRKDAY
                     QDQSEKTPITLEQSRARTLAIMDCYLMRHGEAVSGQPDQQRSLSNRGRAEVSAIAQRA
                     RRRGVLVAWIYHSGILRAQQTAEVVADVLLPAEGLREISGLYPDDDPSIFKAQADEFA
                     QSVLLVGHLPYLSRLAGLMTTGDSERFVISFAPATLASLTRQGDHWQFNWSLSTASP"
     gene            5403..6689
                     /locus_tag="VMZ02_00970"
     CDS             5403..6689
                     /locus_tag="VMZ02_00970"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HUR70524.1"
                     /translation="MASETLTIIDNRTGKKYEIPIKHGAIKATDLFQIKISEDEGIVS
                     YDPGFMNTASCQSKVTYIDGDKGILRYRGYPIEQIAEKSTFLETAYLILNGELPTQAQ
                     LDDFSHHVTFHSIIHENIKKLIDGFHHDAHPMGMLISTVGALSTFYPNAKKIFDAQNR
                     REQTYRLVGKMPTLAAFAYRHSLGLPYSYPDNDLSYTGNFLNMLFKMTELKYKPNPVL
                     ERALDILFILHADHEQNCSTNAMRGIGSSQPDPYSSVAGAAAALYGPLHGGANEAVLR
                     MLMEIGSKEKVADFIEKVKGGSGRLMGFGHRVYKNYDPRARIIKNIADQVFDVMGKNP
                     LLEIALELERIALSDDYFIKRKLYPNVDFYSGLIYQSMGLPMDMFPVLFAIPRTVGWI
                     AQWEEMLTDPDQKLARPKQIYLGAPTRDFIEIGKRR"
     gene            complement(6694..7860)
                     /locus_tag="VMZ02_00975"
     CDS             complement(6694..7860)
                     /locus_tag="VMZ02_00975"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUR70525.1"
                     /translation="MPRPQPAPLSPEAKSARVDGRETAATSIARNTPTVLPPPPSDNR
                     LLELLEKDIDKALEQPKERRRLQFSKDVIEHPRVRQFITQYSISNKNYFQTLLARSGR
                     YMPIIAKVLSEEGLPEELAYLALLESEFQVDTASQAGAVGLWQFVPSTARQYGLRIDD
                     WVDERRDPVKSTKAAAAYLKDLHAYYGRWYLVTAAYNAGPGIIDKALQTSRASDFWGI
                     KEKAQLSQETRNFVPKFVAIALIATNPKKYGFNNLRYEEPLEFDEVDAEGLLKFDAAA
                     EMAEADAAIIKELNPALLRNLTPPGARGYRLRVPVGKGLVFAKAGEYQRSKESEPVRL
                     VTHEVKRGDTLASIAARYGQAVSSLMELNGLTSARLQIGQKIRIILDGIRGSLR"
     gene            8706..9536
                     /locus_tag="VMZ02_00980"
     CDS             8706..9536
                     /locus_tag="VMZ02_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70526.1"
                     /translation="MISGYHDAVDGVVLMDIGMDKLREHLRPTYRAHRTITHGGVQPN
                     IIPEMGQIWWFVRDATGPWAKENFDKLVNIGKGAALMTGTTMEMEPYGAAWPSLGNKA
                     IAEAVQKNIDMVGMPKWTEEENNFAKELQKSMGQKDIGLSEKPAPLVTGRQGSSSNDI
                     GDITWVVPTGTIRFPSVVPGIQAHHWSAGITPTMSIGHKGAVAGAKVIAASVLDLLTS
                     PELRAAATKQFEQDTKDSKYFSLLPEGAKPPLDLNKEMMEKYRPAMQKFYLNKRAEFK
                     "
     gene            9595..>9830
                     /locus_tag="VMZ02_00985"
     CDS             9595..>9830
                     /locus_tag="VMZ02_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70527.1"
                     /translation="MRLWRQHFGAACLVLILAVGTSPVSAEDVTVSFASLGAAYMDHL
                     VAIERGYMAEEGLSVKIIRSGGGSATQTLLSGQL"
BASE COUNT         2300 a         2485 c         2744 g         2301 t
ORIGIN      
        1 aaagatggag gttagtcatg gccacgacaa tacccgaatc atttaaggac cttcttaaca
       61 aagtcgcatt cgcacatctg gcaacggtaa tggcggatgg ccggccacag gtgacgccgg
      121 tatggtttag tttcgatggc acgcattttt gggtcaactc ggcaaaaggc cggctcaagg
      181 acaaaaatat gcgccgcaat aaatatgtgg cgctgtcgat ccaagatccc gacaatcctt
      241 atcgttatct ggcgattcag ggcgaagtcg aggagatcac tgagaacggt gccgatgcgc
      301 acatcgatac gttagctaag cattacttgg gcaaggacaa atacccattt cgcgcgcctg
      361 gcgaggtgcg ggtcatctat aagatacgtc cgcagaagat ttctacgatg agttgagaaa
      421 attcgacaat ccacacctga ccgccaccga gctcgtgcag cggggctggt gctggtagcc
      481 gagggaaaat gaccgcagcg caggacgttt tatcctgcga tgctgtgcgt ctcgatcata
      541 aggtcatatc agaatagcct gattgtcaga gcgattgagc gagtccgctg ttcttgagcc
      601 gttggtaggc tttggacgcg atcgcgacat ccatgatgcc ggtaccttgc agcttgaaaa
      661 gggtaatttg ccgatcatcg ctacggccga cagccttacc tagcaatagg ttggagattt
      721 cgtgaacctt gtcccagcta ataactttat cccgcactgc gcggaaaata tcgctttcat
      781 tgacacagac ggtttccttt gaggttacaa atatcacgtc ggcgcggcgt aggactactt
      841 cgtctagttc ctggcgcgtg cgcgttttat cgccgttggc aatcgaggtg atatgcatgc
      901 cgtcttttag actcttgccg tcgaataccg gtgtacggga atttgtcgcg gcaattagca
      961 catccgcgtt tgccatagct tcttcagcgc tgccggcgat gtgaagcgga atctccaact
     1021 cctgcgccat ctttgccgcg aacgaggcac agcgctcctg agtcggactg taaacccaac
     1081 cttcggtcaa tttcctaact gtcgccacag ccttgagttg cgtctccgcg tgcacggcgc
     1141 tgcccaagac tgcgacgcgg ctcgcatcgg aacgcgccag atatttcgcg ccgatgccgc
     1201 cggcggcgcc cgtgcgatac tcgttgatcg aacaatcttg aaacaacatc agtggtgcgc
     1261 cgttttcggt gctgaagatc atgacgtttt cgtggtgaat cttacgattt aggtcggttt
     1321 gcatgtaagc acccatggca tcgtggaccg atccaggcag gatgccgaaa atcatcccgt
     1381 tgggatgatg aatgcgccgc cggggcagtt catagccgcg gccacaagcg atctccttca
     1441 aggtctcctc gatggtttca atcatttcgc tcatagagag gatttgattg atgggcgtgt
     1501 ttttgaggaa gagcatgacc gttctcctct gccagccata ctgcaagcgc aaagagaccg
     1561 tcaagtcaaa ttgtgttgat tgatcttcgc gtgcgtctgg ccccgatcag cggctagcta
     1621 gttggtgttc caaatggccg gcgtagcgcg acatggcgta gctacatagc cagtagacga
     1681 ttgcgatgaa aaggtagagc tcgaacgggc ggatttcgcg gttattgacg atctgggcag
     1741 ctttggtgag atcgacgtag ccgatgattg aggccaagga agtatctttc aagagtacga
     1801 taaattgagt gaccagcgcc ggaatcatgt tgcgtaaggc ttgcggcaaa actacatggc
     1861 gcattagttg ctttttggaa agtcccgtgg cgattgccgc ttcgacttga ccgcgcgcaa
     1921 cagactggat gcctgcgcga acgatttccg cgagataggc tgcttcgaag acaacaaagg
     1981 cggtaagtgc tacggcatac tccggcaaag atttacctat gagggtcgga ataatgaacc
     2041 agaaccaaaa gatcaccatc accaatggta cgccgcggaa gaattctacg taaactttcg
     2101 ccgggccgta gagccatggc gaatgcgata agcgcgctag gccaagtatt attcccaata
     2161 tggatcctag aataatggca gggacagcca aacgcagggt gccgccggta aacgcgccga
     2221 tgccgaaaaa tccttgagcg atcaggaagg caaaattatt gctgatgaca cgaaagtcca
     2281 tctcacgatg ttccggtatg tccttggacg atcaacccgg gaatcgcgac gcgccgctcc
     2341 acgcgggcca tgattgaggc aatggtgagg cataaagcga ggtaaataac cgtgccggcg
     2401 gccaatgctt caaacgcttt ggctgtgtag gtttcgatct ggcgggtttg aaacgtcaac
     2461 tctgcgactc ctatggtgag tgcgacagag gagttcttga gcaggttaac ggtttcgttg
     2521 gtcgctggtg ggatgattaa gcgcagcgcg acagggatga taattaggcg ataagcctgg
     2581 ccaacggtga ggccggtgga tagcgcggct tccaattggg ttttcggtat cgactgaata
     2641 cccgcgcgca tcacttcgga aaagcgcgcg ccgtggtaaa cgcccaaggc gaatactgcg
     2701 gcccaaaact cggcgccatg atcgaaaagc cagttctgta tcgcttgcgg caataacggc
     2761 gggacactga agtagccaaa aaacatccac actagcaacg gtacattgcg aaaaaactca
     2821 acgtagctgc tggcaagcca acgcagcggc gcaatcgcta ctgtcctcag tgcacccgcg
     2881 cagatgccga gaactattgc gatgattagt cccacggcga aaatttgcaa tgtggtaata
     2941 accccctgaa gcagccagcc gccggactgg ccggtccaca gagtgctcca atcgaagtgg
     3001 taagtcattg gtgcaggcgc gcgtcacaat gctgaaggat catttagtag ctatgaacgc
     3061 atttctaatt cgcgcctcat ggctatttca tttcggcact acttgcagct gcaggaagcg
     3121 cttgttctcc ggcgtgagcg ggtagggtac ttcgcctttg gccccaaacc acttttcgta
     3181 gagatcgaag tagagtccgg aggagattgc gtccatcaag cccacgttga ccaacagacg
     3241 aaagtcggaa tcgtttttac gcatcgccat cgcgtatggg tcgtaggaga agaaatcgcc
     3301 gaccacagtc cagtcatcag gtttaggcgc cttggctctt aagccggcga gttgaatgcc
     3361 gtcgttggta tatgcgtcga cttgacgctg ggaaagcgct tgaaacgcgg cgggttgatc
     3421 ctggaattcc acgagcttgg cgttcggtac tttttcacgg aggattttcg cgttcgtcga
     3481 gccttgctgg gcggcgacgc gtttgccggc aatgctctgg atgcctttga ccgggctgcc
     3541 tttgcgcacg agaaactgcg caccggtaat aaagaaggtt aggctgaagt cgacttgttc
     3601 gcgccgcacc tgagtgtcgg tcatggtttc ggctattaaa tccactgcgt ttgaggttag
     3661 cagcgggatt cgtgtcggca cggtcgattc tttcttctcg agtttgattt gtttgttcaa
     3721 ctttttgctg agggccggca gtatcccttg ctcaaccaga ttgattgaga agccaaccca
     3781 ctcgttgttc ttattgacga aggcaaacgg cggcgagccg gtgcgcgtgc cgacgacgaa
     3841 tgtgccactt ttatcgattt tctccagcgt gctttcggcg aagctaaggt tcgtcgtctc
     3901 tgtcgcaagt acgactgcga caaggaatgc aaccgatagt ttcatgatag ggctccttaa
     3961 tgtgaaagga ttttactcaa aaattgctgg gctcgagccg attggggtgc ggcaaaaaaa
     4021 gcttctggtt cggcggtttc aatgattttc ccctgatcca taaagacgat ccgatgggcc
     4081 acacgccgag caaaacccat ttcgtgggtg accaccacca tggtcatgcc gtccttggct
     4141 aaatccgtca tgacctcgag cacttcgttg atcatttcgg gatcgagggc tgaagtcggc
     4201 tcgtcgaaga gcatgacctt gggtttcatc gccaatgcgc gagcaatggc gacgcgctgc
     4261 tgctggccgc cggaaagatt cgccggatag acctgcgcct tatcgggaat gccgacacgg
     4321 tccaataatt ccttagctat tctttgagct tcggtgacag tgatgtttct gactttgact
     4381 ggagccagcg tgatattctg caagaccgtc atatgcgggt agagattgaa cgactgaaag
     4441 accatgccga cattggagcg cagcttggcc gcgtccaatt tagggtccga caatgacaga
     4501 ccgtcaacga caatctcccc cgattgaatg gtttcgagtc ggttgatgca gcggatcatg
     4561 gtgctcttgc cgctgccgct cgggccgcaa accacgacca cgtgccctgc ggcgatcttt
     4621 agttcgatgt cgctcagcac atgcagggcg ccgaaccact tgttgacgtt gtgaaattcg
     4681 atcacggtcg ttgcagactt gcagaaagac tcggttgcaa aacactggtc ggcatactat
     4741 atcaacaacg gctttttgca agaataattg gcgctttcgc cgatctccgc aaagacgctt
     4801 atcaagacca atcagaaaaa acaccgataa cgcttgagca atcgagagcg aggactttgg
     4861 caatcatgga ctgttacttg atgcgccatg gggaagctgt ctcagggcag cctgatcagc
     4921 aacgctcgct gtcaaacagg gggcgcgccg aggtgagtgc gatagcgcaa cgcgcgcgca
     4981 ggcgtggggt tttggttgct tggatttatc actcgggcat cttgcgggcg caacaaacgg
     5041 ccgaagtagt tgccgacgtg ctgcttcccg cagaaggtct ccgggaaatc agtggtctat
     5101 acccggatga cgacccttcg attttcaagg cgcaggccga cgaatttgca caatccgtgc
     5161 tcctagtcgg tcatttgcct tatctaagcc gcctcgccgg tttaatgacg accggagatt
     5221 cggaacgttt cgtcatcagc ttcgcgccag caaccttggc ttcgctgaca cggcaaggcg
     5281 atcattggca gttcaactgg tcgttgtcga ccgcatcgcc gtgagccggt tgaccggcgc
     5341 agagcgatag tctaaaaaag aaaaaggctt gcgagttagc gcgcgctgaa aggtacatgc
     5401 caatggcttc agagacttta acaattatag ataaccgtac cggaaaaaag tacgagatcc
     5461 caatcaagca cggcgcgatc aaagcaaccg atctattcca gataaagatc tctgaagacg
     5521 aaggcatcgt aagttatgat ccgggcttta tgaacacggc atcctgccaa agtaaagtca
     5581 cctacatcga cggcgacaaa ggaatcctgc gctaccgcgg ctatccgatc gagcagatcg
     5641 ccgagaaaag cacattcctg gaaaccgcct atctcattct caacggcgaa ctgccgactc
     5701 aagcacaatt ggatgatttt tcccatcacg ttactttcca ctcgatcatt catgaaaaca
     5761 tcaaaaagct catcgacgga tttcatcacg atgcgcatcc gatgggcatg ctgatcagca
     5821 ccgtcggcgc gctgtcgacg ttttatccca acgctaaaaa gattttcgat gcgcagaacc
     5881 ggcgcgagca gacctatcgg ttggtcggca aaatgccgac tttggcggcg ttcgcctacc
     5941 gccacagcct cggattacct tattcttatc cggataatga cttaagctac acaggcaatt
     6001 ttttgaacat gttgttcaag atgaccgagc tcaagtacaa accaaatcct gtgctggagc
     6061 gggctctcga catccttttt atcttgcacg ccgatcacga acagaactgc agcaccaatg
     6121 ccatgcgcgg tatcggcagc tctcagccgg atccttattc gtcggtggcg ggtgcggcgg
     6181 cggcgctcta tggcccgctc catggcggcg ccaacgaagc ggtgttacgc atgttaatgg
     6241 agataggttc caaggaaaaa gttgccgatt tcattgaaaa ggtcaaaggc ggatccggcc
     6301 gcctcatggg cttcggccat cgggtttaca aaaactatga cccgcgcgcg cgaattatca
     6361 aaaatatcgc tgatcaagtt ttcgatgtaa tgggcaagaa tccactgctc gaaattgcct
     6421 tggagctgga acgcattgcg cttagcgacg attacttcat taaacgcaaa ctatatccta
     6481 atgtcgattt ctattctggc ttgatctacc aatcgatggg ccttccgatg gatatgtttc
     6541 cagtcctgtt cgcgatacca cgaacggttg gctggatcgc ccaatgggaa gaaatgctca
     6601 ccgatccaga ccaaaagctg gcccgcccca agcagattta tttaggagcg ccgacgcgcg
     6661 attttattga aataggtaaa cgcaggtagg ccgttagcgc agagaaccgc ggatcccgtc
     6721 aagaataatc cgaattttct gaccgatttg caaacgtgcg gaggttaagc cgttgagctc
     6781 catgagtgag ctgacggctt gaccgtatct tgcggcgatc gatgcgaggg tgtcgccccg
     6841 tttgacctcg tgagtgacca gccgcactgg ttcagattcc ttgctgcgct ggtattcgcc
     6901 ggctttggcg aataccaggc ctttgccgac agggacgcgc aagcgatacc cgcgtgctcc
     6961 tggcggagtt agattgcgga gcagcgcggg attgagttct ttgataatcg cggcatcggc
     7021 ctctgccatc tcagctgcgg catcaaactt tagtagccct tccgcgtcga cttcgtcaaa
     7081 ttccagcggc tcctcgtagc gcagattatt gaatccatat tttttaggat tagtggcgat
     7141 caacgcgatg gcaacgaatt tgggcacgaa gttgcgggtt tcttgactta gctgcgcctt
     7201 ttctttgata ccccaaaaat ccgacgcgcg gctggtctgc agggctttgt ctatgatacc
     7261 cggtccggcg ttgtatgccg cggtgaccaa ataccagcgt ccgtagtatg cgtggagatc
     7321 tttcaaatag gcggcggcgg ctttcgtgga tttcactgga tcgcgccgct cgtcgaccca
     7381 gtcgtcaatg cgcaatccgt actgacgcgc cgtgctcgga acgaattgcc acagtcctac
     7441 tgcgcccgct tgggatgccg tgtctacttg aaattcgctt tcgagcaggg cgagatacgc
     7501 caactcttca ggcaatcctt cctcgctcag gaccttggcg atgatcggca tgtatctgcc
     7561 agagcgtgcc agcaatgttt ggaaataatt tttgttgctt atggaatatt gagtgatgaa
     7621 ctgacgcacg cgcgggtgtt caattacatc tttggagaac tgcagacgac ggcgttcctt
     7681 gggctgttcg agcgctttgt cgatatcctt ctctagaagc tctagtaggc gattgtcgct
     7741 cggcggcggt gggaggacgg ttggagtgtt tcgcgcaatc gaagtcgccg ctgtctcgcg
     7801 gccgtcgacg cgagcgctct tcgcttccgg cgaaagcggc gcaggttgcg gcctgggcat
     7861 gggcgggagc gcgacctcta cagtaggttt cgaaatttca ttgttcgtcg gaagttgcgg
     7921 caaaatcgct ggagtgggca atatcggatc gggagttaac ggaagtacgg ggacaatgga
     7981 cgcgggtgct gaggaagcaa ccacgctgtg ataccggcta tctctgctgg cgcaacctga
     8041 aacaccaact attgccaaca gcagatgaaa gagaatcctg ctagaccgca tagacacctt
     8101 gaaccgaggc taggctagcg aaaatagcta tggggcacaa cccttgagag gagaaattta
     8161 tagaaatttt tcgcgtgatt ctgcgatttg tacgcctcag aaaaaaaggc gaagcgcaat
     8221 gagaaatatt ttcttaatct cggtactgag attcgaaatt cgagaaaatt acgatcgatt
     8281 acgctaaaga cttctctttt gagccaatgc tttacttctc gcttcgaaaa aatccggcga
     8341 atggcctacc gcgcttttac tggagcactg tgggtgaacg acgatgtttt gcaatgattg
     8401 aattctacta gctacgattt atttgagggt aattattttt tgactaatgt ctttggaaat
     8461 aagggtggtc agatcacggc tagattaaaa gcactcgtgt ttcggctctt tttgcgagga
     8521 tggagcagca attgctgatc attaaatctt cgatcaacgt ctgtgcttga ggcgtcattc
     8581 cggatttagc ggtacgcgtg accgatgagg tgcgtcggtg acggaagccg agattttttc
     8641 gatccgtcca tgctcatatc cggacctata aagttctgcg cttgcttcta ttgcattagt
     8701 tagtcgtgat aagtggttac catgacgcgg tggacggcgt tgtgttgatg gatatcggca
     8761 tggacaaatt gcgcgagcac ctgcggccta cctaccgcgc ccatcgcacg attactcatg
     8821 gcggtgtgca gccaaatatt attcctgaga tgggacagat ctggtggttc gttcgcgatg
     8881 ccactggacc ctgggccaag gagaatttcg acaagttggt caacatcggc aaaggcgccg
     8941 cgttgatgac cggaaccaca atggagatgg aaccctacgg cgccgcatgg ccatcgctcg
     9001 gtaacaaagc gatcgctgag gcggtgcaga agaacatcga catggtcggc atgccgaaat
     9061 ggacggaaga ggaaaacaat tttgccaagg agttgcaaaa atccatgggc cagaaggata
     9121 tcggtctctc ggagaaaccg gcgccgctcg tgactggccg ccaaggttcg tcgtccaacg
     9181 atatcggcga tattacctgg gtggtgccga cgggaacgat tcgtttcccg tcggttgtgc
     9241 ctggtattca ggcgcatcac tggtcggcag gcatcacgcc gacgatgtcg atcgggcaca
     9301 agggagcagt cgccggagca aaggtgattg ctgcgtcggt tctggatttg ctgacatctc
     9361 ctgaattacg agctgccgcc acgaagcagt tcgagcaaga caccaaagat agtaagtatt
     9421 tctcgctgct tcccgaaggc gcgaagccgc cgctcgatct caacaaagag atgatggaaa
     9481 aataccggcc ggcgatgcag aagttttatc taaacaagcg ggccgaattc aaatagacca
     9541 agcaaccgac aatgtcgaga tagacgagtg aagttaatgt ttagcagaca aataatgcgg
     9601 ctttggcgac agcactttgg cgccgcctgt ttggtgctga ttctcgcggt ggggacgtcg
     9661 ccggtgtcgg ccgaggatgt gaccgtctcg ttcgcgtcgc tgggcgcggc ctatatggat
     9721 catctggtcg ccatcgagag aggctacatg gcggaggagg ggttgagcgt caagatcatc
     9781 cgttccggcg gcggcagtgc gacgcaaacc ttgttgtccg gccaactgca
//
LOCUS       DATLCH010000036         5992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_874338, whole genome shotgun sequence.
ACCESSION   DATLCH010000036 DATLCH010000000
VERSION     DATLCH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874338"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VMZ02_00990"
     CDS             <1..1008
                     /locus_tag="VMZ02_00990"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR70528.1"
                     /translation="ICKERGSGAVNVGIYRHQKQGKRQLGLMVNPANHANYVRAEYED
                     HNEPMEVALCIGHHPALNMAAVSKQAGVGSELEIAGAFLGEPLEVVKAETVDIMVPAH
                     CEIIIEGIVPPKKRQYEGPFGEWPHYYYKEGEQPYIQVTAITMKKNPIYQSIHSAHIE
                     HNIIGAAPRLGSLLKRVQEVVPSTTAVNLPISGAARSHCYISVKKRAEGDPKQAALGA
                     IVTDPNIKHVIVVDDDVDVFNENQVLWALAMRFQADRDLVIIPNIIGSHLNPTAYGYN
                     RMERGPMETKLIFDATKPLPPYDFPKEAQAPDEVLNKIDLNRDTRAYEPGKDDRAITG
                     K"
     gene            1151..1939
                     /locus_tag="VMZ02_00995"
     CDS             1151..1939
                     /locus_tag="VMZ02_00995"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70529.1"
                     /translation="MVEIERVSKSFYKTVKDRTTEVQALSNVNFSVHANEFLSIIGPS
                     GCGKTTLLKMIDGLIPYDSGKISIGGKQITAPGPDRAVVFQTFALLPWRTVLANVEFS
                     LELRQINTRERTEIARDCLKRVGLGDFENHYPHELSGGMQQRAGLARALAVNPSILLM
                     DEPFGAVDAQTRQLLQEELLELWQRERKTVIFITHSMDEAVYLSDRVVVMTPRPGRVA
                     EVLEVPLARPRITEDVRRDPKFAELTNYIWHSLKKAMDNGHAGV"
     gene            2020..2775
                     /locus_tag="VMZ02_01000"
     CDS             2020..2775
                     /locus_tag="VMZ02_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70530.1"
                     /translation="MHSQFNINALSILSLAAVWEISGRLFDSVLIPPLTKIGAAWYKL
                     LLGGKLLPNLAASLWTMIIGFVLAVLVGIVIGLLMGRFRAVEHFLDLYVNSLMSAPTT
                     AFVPVLILWFGLGVQSRIAVVFLFAVFVVIINTMTGVKQVDNVLLEMARSFGAKEKET
                     FLKIILPAAMPAIMAGVRLAMGRAVKGIVTAEMLLTLTGIGAMIMQYGSAFATDALFA
                     VILTILIVAMIAMKLVQMLDRRLTGWKVEVAVE"
     gene            2800..3783
                     /locus_tag="VMZ02_01005"
     CDS             2800..3783
                     /locus_tag="VMZ02_01005"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70531.1"
                     /translation="MTRSCVRISAFVVGFAVALLSAGPAQAQKKVRFAYPSSADMGDV
                     PSLLAWEQLKKQGIEVVPTFFPRTDLAVQAVVAGEADIGSAAGIAVVKAVESGMNVRV
                     IGEQVRNEWQLVAPVSFKDPKQLDGKRVGYHAPITVTEALVKWMAQHYKITPNWLIIP
                     GSDVRAEALMRGQLDATPAEIVDVLNILNAKPGQFHVLISYAKIFPQLIGSMYFSRAD
                     YLQKEASTVDGVIEAILRAHRNAEERPASVRENALRLLPETKPELIDAIAASYKDLRI
                     WDINGGAGKERGEASIKFFEDAGLMKKGAVSFAQSFDTGPIDRVLKKIGRK"
     gene            3860..4603
                     /locus_tag="VMZ02_01010"
     CDS             3860..4603
                     /locus_tag="VMZ02_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70532.1"
                     /translation="MKPNTLRLLSLITLGMAWEIAGRTGNARLLPPISKVMSAWFDLL
                     LTGQLVNALAVSLQALTLGFILSVLFGVPLGLLMGRYRRLESFLDIYMTALLTVPMIS
                     FIPFLVIAFGLGLHSRIWIVFLFAFVIIAINTTAGVRNVDPTLTEMAKSFGAKESDLF
                     KKIILPAALPMIMAGIRLGMGRAILGMVTSELILAVVGFGAMLMTFGASFNSPALFAT
                     ILTIVVLAVALLAVIQYLDRRLTPWRAAA"
     gene            4697..5704
                     /locus_tag="VMZ02_01015"
     CDS             4697..5704
                     /locus_tag="VMZ02_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70533.1"
                     /translation="MNKRISHRTFPFITALIAISLAYSDAAAQTAMEKLRVAYTVIAP
                     TQATVWTAKDLGLFAKHGLDVDLVLLVGAPLAIAALVSGETPIVHAGASAVVTSNLQG
                     SGTVLIAGGANRFPYVLFVTDEIKRVEDLRGKKFGVSRIGSADHAAAVTVFDKLGIKE
                     TEITYVQAGNIPARLAAMQGNALQASLLQAPETLKAKEIGFRALLDFTKLDVEWQQNG
                     VAVTRDYIKKRPDTVRRFLRAYVEGSHYNLTNAKGAQKILQKYLAIKDEKSVEEAYNE
                     IVVKLTRRVPYPTEPGIQLFLDQLKVKNPKAGQAKPSDFTDVSFLKELESSGYIDKLY
                     K"
BASE COUNT         1365 a         1742 c         1638 g         1247 t
ORIGIN      
        1 atttgcaagg aacggggcag cggcgcggtt aacgtcggca tctatcgaca tcaaaaacag
       61 ggcaagcggc aactcggatt aatggtcaat ccggccaacc acgccaatta cgtccgcgcc
      121 gaatacgaag atcacaacga acccatggaa gtagcgctct gcatcggtca tcatccggcg
      181 ctcaatatgg ctgccgtcag caagcaggcc ggcgtcggct cagagttgga gatcgccggc
      241 gcctttctcg gcgagccatt ggaagtcgtc aaagcggaaa ccgtcgatat catggtgccg
      301 gcgcactgcg agatcatcat cgaaggcatc gttccgccca agaagcgcca gtacgaagga
      361 cctttcggcg aatggccgca ttattattat aaggaaggcg agcagcccta cattcaagtc
      421 accgccatca cgatgaagaa gaacccgatc taccaaagca tccacagcgc gcatatcgaa
      481 cacaacatta tcggcgccgc accacggctc ggcagtctgc tcaaacgcgt gcaagaagtc
      541 gtgccgagca ccacggcggt caacctgccg atctccggcg ctgcccgctc acactgttat
      601 atctcggtta aaaagcgcgc tgaaggcgat cccaaacagg ccgcgttggg cgccatcgtc
      661 accgatccga atatcaaaca cgtcattgtc gttgacgacg acgtcgatgt gttcaacgag
      721 aaccaagtgc tatgggcgct ggcgatgcgc tttcaagccg accgcgatct ggtgattatc
      781 cccaatatca tcggctccca cttgaacccg actgcctacg gttacaatcg tatggagcgc
      841 ggccccatgg agaccaagtt gatcttcgac gccaccaagc cgctgccgcc ctatgatttc
      901 ccgaaagaag cccaagcgcc cgacgaggta ctgaacaaga tcgacctaaa tcgcgacacc
      961 cgcgcttacg agccgggaaa agatgatcga gcgatcaccg gaaaataatt cagatgaaga
     1021 cgttcaagcc gttcaaatag ttcaaatcgt tcaaaccgta ttcgaacggt ccgagtttta
     1081 ccacgttttg aacggagcga agcgattgaa cggcttgaac gttgtgaacg ggagccgata
     1141 ggcgacaaga atggttgaaa tcgagcgggt ctcgaaaagc ttttacaaga ccgtcaagga
     1201 ccgaaccacg gaggtgcaag ctctgtctaa cgtgaacttt tcggtccacg ccaacgagtt
     1261 tttgagcatc atcgggccga gcggctgcgg caaaaccaca ctgctcaaaa tgatcgacgg
     1321 tctcattccc tacgacagcg gcaagatctc gatcggcggc aaacaaatca ccgcccccgg
     1381 tccagaccgc gccgtcgtgt ttcaaacctt tgcgctgctc ccttggcgca ccgtcttagc
     1441 caatgtcgag ttcagtttgg agttgcgcca gatcaacacc cgggaacgaa ccgagatcgc
     1501 tcgcgattgt ttaaagcgcg tcggcctggg cgatttcgaa aatcattatc cgcacgaact
     1561 ctccggcggc atgcagcaac gggctgggct ggcacgcgcg ctagcggtca atccgtcgat
     1621 cttgctgatg gacgaacctt tcggcgccgt cgatgcgcaa acccggcagc tgttgcagga
     1681 agaattgctc gaactctggc aacgcgaacg caagacggtg atcttcatta cgcacagcat
     1741 ggacgaagcg gtctatctct ccgatcgggt cgtcgtcatg acaccgcgtc ccgggcgagt
     1801 cgccgaagtg cttgaggttc ccctggctcg tccgcgcatc acggaagacg tccggcgcga
     1861 ccccaaattc gccgagttga ctaactatat ttggcacagc ctcaagaaag cgatggacaa
     1921 cggccatgca ggcgtatgac gttcggcgcg gctatgaacg cggcattgat tcggattgct
     1981 tccctgcttg cgagagaagg ctagaacgag ggagctacct tgcatagcca gttcaacata
     2041 aatgcgctgt cgattctctc cctcgccgca gtttgggaaa tttccggccg cttgtttgac
     2101 tcggtattga tcccgccact gaccaagatc ggcgccgcct ggtacaaact cttgctcggt
     2161 ggcaagctct tgcccaatct cgccgccagc ctatggacga tgatcatcgg tttcgttttg
     2221 gcggttctcg tcggcatcgt gatcggcttg ctgatgggcc ggtttcgcgc cgtcgaacat
     2281 ttcctcgatc tctacgtcaa ctctctaatg tcggcgccaa ccaccgcctt tgtgccggtg
     2341 ttgatcctct ggttcggtct cggcgtccaa tcgcgcatcg ccgtggtttt tctcttcgcc
     2401 gtgttcgtgg tgattatcaa caccatgacc ggtgtcaaac aggtcgacaa cgttctgctt
     2461 gaaatggcgc gctccttcgg cgccaaagaa aaagaaacct tcttaaaaat cattctgccc
     2521 gccgccatgc cggcgatcat ggccggcgtc cgcctcgcca tgggccgtgc cgtcaagggc
     2581 atcgtcacgg cggagatgct gctgacgcta acaggcatcg gtgcaatgat catgcagtac
     2641 ggttcggcct tcgccaccga tgccctgttc gccgtcatct tgacgatttt gattgtcgcg
     2701 atgatcgcca tgaaactggt gcaaatgctt gatcgacgtc tcaccggatg gaaagtagaa
     2761 gtcgccgtcg aatagttgat taaactaggg gagggttcta tgaccaggtc gtgcgtaagg
     2821 atttctgcct ttgtagtcgg gttcgccgtc gcactgttgt ctgctggacc ggcacaggcc
     2881 cagaaaaaag tccgcttcgc ctacccgtcg tcggcggaca tgggtgacgt gccgtcgctg
     2941 ctcgcatggg agcagctcaa aaaacagggc atcgaagtgg tgccgacgtt tttcccgagg
     3001 accgatctag ccgtgcaggc tgtagtcgcc ggtgaagcgg atatcggttc cgccgccggc
     3061 atcgccgtag tcaaggcggt ggaaagcggc atgaatgtca gagtaatcgg cgagcaggta
     3121 cgcaacgaat ggcagctggt cgcgccggta tcgttcaagg accccaagca gcttgacggc
     3181 aagcgggtcg gttatcacgc gccgatcacg gtcaccgagg ccctggtcaa gtggatggcg
     3241 cagcactaca agatcacgcc caactggttg atcattcccg gctcggatgt ccgcgccgaa
     3301 gcactcatgc gcggccaact ggacgctacc cccgcggaga tcgtcgacgt gctgaatatt
     3361 ttgaacgcca agcccggaca gtttcacgtt ttgatctcct atgcgaaaat tttcccgcag
     3421 ctgatcggct cgatgtactt ctcacgcgct gactatctgc agaaagaagc tagtaccgtc
     3481 gacggcgtca tcgaagcgat cttgagagcg caccgcaatg ccgaagaacg gccggcatcg
     3541 gtgcgtgaga acgcgttgag gctgctgcct gaaaccaaac cggaattgat cgatgccatc
     3601 gccgcaagtt acaaagatct gcgcatctgg gacatcaacg gcggtgccgg caaagagcgc
     3661 ggcgaagcca gcatcaaatt ttttgaggat gccggcctta tgaaaaaggg cgcggtcagc
     3721 ttcgcgcagt cctttgacac cgggccgatc gatcgtgtgc tgaaaaaaat cggtcgcaaa
     3781 taaccgcagc gcgactcgat ggtgttcaac gtagtgggtg ggatgggtgt caatcccacc
     3841 tcgctgtgac gacgcgagca tgaaaccgaa tactctgaga ctcctctcgc tcataaccct
     3901 cggcatggct tgggaaatcg ccggccgcac cggcaacgcc cgcttgttgc caccgatctc
     3961 caaggtaatg agcgcgtggt ttgacttgct gctcaccggt caactcgtca atgccctcgc
     4021 cgtgagcctg caagctctca cccttggctt cattctctcg gtgctattcg gcgtcccatt
     4081 ggggctgctc atggggcgct atcgtcggct cgaatcgttt ctcgatatct acatgaccgc
     4141 gctgctaact gtgccgatga tttcgttcat cccctttttg gtgatcgcct ttgggttggg
     4201 attgcactca cggatatgga tcgtgttcct ttttgccttt gtcataatcg ccatcaacac
     4261 caccgccggc gtgcgcaacg tcgatccgac gctcaccgaa atggccaagt cgttcggcgc
     4321 caaagaaagc gatctgttca agaaaattat tttgcccgcc gccctgccga tgatcatggc
     4381 gggaatccgc ctcggcatgg gacgcgccat attgggcatg gtcactagcg aactgatcct
     4441 cgctgtggtc ggcttcggcg ccatgctgat gaccttcggc gcgtcattta attcgccagc
     4501 gctgttcgcg acgattctga ccatcgtagt actcgccgtc gcgctattgg cagttattca
     4561 atatcttgac cggcgcttaa cgccctggcg tgcggccgcg tgacacttca taaatctaca
     4621 ggcttgagtt acaaagagac cgatccgact gggcggcgtt tacggtcgat cagcgcgacc
     4681 cggagaatat tgcctcatga acaagcgaat ctcgcatagg acgtttccat tcattaccgc
     4741 tttaatcgcc atttcgctgg cgtattctga tgcggcagcg caaacggcaa tggaaaaact
     4801 gcgcgtggca tacacggtca tcgcaccgac tcaggcaacc gtctggaccg ccaaggatct
     4861 gggtctgttt gccaaacacg ggctcgacgt cgacttggtg ttgctcgtcg gtgcgccgct
     4921 ggcgatcgcc gccctggttt cgggggaaac accgatcgtc cacgccggag cctccgccgt
     4981 ggtcaccagc aacctgcaag gttcaggcac cgtcctgatt gcgggcggag ccaatcgttt
     5041 tccctacgtg cttttcgtca ccgatgagat taaacgagtg gaagacttga gaggcaagaa
     5101 attcggcgta agccgcatcg gcagcgccga tcatgcggcg gccgtcaccg tctttgacaa
     5161 actcggcatc aaagaaaccg aaatcaccta tgtgcaagcc ggcaatattc cagcgcgctt
     5221 ggcggccatg cagggcaacg cgctacaggc aagcttgttg caggccccgg aaacgctaaa
     5281 ggctaaagaa atcggtttcc gcgcgctgct cgatttcacc aagctcgacg tagaatggca
     5341 gcaaaatggc gtcgccgtta cgcgcgacta catcaaaaaa aggcccgaca ccgtgcgccg
     5401 cttcttgaga gcctatgtcg agggcagcca ttacaacctg accaatgcca agggtgcgca
     5461 gaagatcctg caaaaatatc tcgcgatcaa agacgaaaag tccgtcgagg aagcttacaa
     5521 cgaaatcgtc gtcaagctga ccaggcgcgt gccctatccg accgagcccg gcatccagct
     5581 ttttctcgat caactaaaag tgaaaaatcc aaaagccggc caggccaaac cgagtgattt
     5641 taccgacgtc agttttttga aggaactcga gagcagcggt tacatcgaca agctctataa
     5701 gtaaacgtgt gcgaaacccg aacgacgaac gattcgaagg gaaacgctgc tcgcaaagag
     5761 tgcggcgcgc cgcgtccgtg tctcacgatt gaactccccg ggattccagc tgcaacgcta
     5821 aaacccgtat gatatagcct gacgcgattt cgctgcagcc gcggaataag taaaccgatc
     5881 ccaagccatg gcccaaacgc aaaacgatag cgttattcga gtcgaaaacg tctggaaaac
     5941 tttccagacc acagataccg acaaagtcgg tgcgttggag aacgtctctc tc
//
LOCUS       DATLCH010000037         5764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6028065, whole genome shotgun sequence.
ACCESSION   DATLCH010000037 DATLCH010000000
VERSION     DATLCH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6028065"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VMZ02_01020"
     CDS             complement(<1..243)
                     /locus_tag="VMZ02_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70534.1"
                     /translation="MATPSHDHAAKEVVELRQEIERHNYQYHVLDNPLVSDAEYDRLF
                     RRLLELENEFPDLVLPDSPTLKVGAPPLQKFTTVQHT"
     gene            394..2106
                     /locus_tag="VMZ02_01025"
     CDS             394..2106
                     /locus_tag="VMZ02_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR70535.1"
                     /translation="MAILSGCATAVPNGQLTPDALIWSPEGPFSKTAKVDIPDEAGAF
                     NHFLKGQLLLIDGEFEPALKEFEAASQASPTDGFLRYRLAQLYLRRGDLKRAMGEAES
                     AAKLEPNSVDYHLLLAGLYSAVGDNQRGLAEYNEVLKLDAKNPEALLYAGALYLQTED
                     LARATQRLDELLQLDDNSALGHYYLGRVRAKSKLYLGAEQSFRKALELNPKSEAVLMD
                     LALVYELEGKTEDAIQTYQRLLQVNPQHVWSRRRLGELYVGQNKLDEALRQFERLEGA
                     ETDPRDTRIKIGLISFEKGDYDRAATEFNLVLAGDPENDRARYYLGATYIELKANDKA
                     LEAFERIPEKSEYYADARVQAAQLYERKEQTPKAIEILQTVIKKLNDRKEIHAYLSVL
                     YRKTKDFPRAIQSMERVVALDPKNDEAHFQLGALYDESKIKDKSISSMKKAIELNPKN
                     APALNYLGYTWAEMGVQLDEAEDLIARALKIAPNDGFYIDSLGWVYYQKGDYPRAVEQ
                     LERAVELTVDDPTIIEHLGDAYEKAGRIDRAVARYRESSKLSKDPEQSKRLREKIQRL
                     EKRI"
     gene            2103..2924
                     /locus_tag="VMZ02_01030"
     CDS             2103..2924
                     /locus_tag="VMZ02_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HUR70536.1"
                     /translation="MTVPFRRLSSVYPTVANFLFAVLALAVVGCATTAKPPAVSQLPV
                     PQWESDKLIESIAQRRQQFRSVRALARVNYVGPEGKHGFEEAVMVQRPDRLRLETLTM
                     LGAILIVTANNQEIIGYHPREGVLVRGRSSKENLQRLTQIPLELEEITALLLGLPPVE
                     PSASWRQEGNSLSYSLNGRTKDNLTFESQLPVPTQWQRFNGTGALELTARFSDYTKTP
                     AGLFPSKINIEAPLQGKKLEIRFQEPEFNAILPAESFTQQKPVHVQEVPIEMIGS"
     gene            2928..4700
                     /locus_tag="VMZ02_01035"
     CDS             2928..4700
                     /locus_tag="VMZ02_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HUR70537.1"
                     /translation="MSCKGWRLILPLLCFVGCGDKTEVKIPDVSPAANQEISGKPVTG
                     DWLVMHSLSDPEQLNPLTSNDSASSEIIELIFEGLLSRDPRTLAFKPVIAEARPEVSP
                     DHLSYTFKIRKDAHFQDGRPLTGEDILFSVKAIKCPFVNAPFLRVYFNSLIDAELIDP
                     YTIRFRTKEPYFLNESVLGGISALPRHYYDPENLLARVSVKQLDQDPQKLPPQVKQFG
                     EQFNKNYSRNPMGSGPFKFGSWKTGREVELIRDDQYWGRGKADIDQVYIDRLKFRIIN
                     NPDAALIRLKSGSLDYIEALQPVQYVRGTGSQRFKQEFQTYEYYAPTYTYIGWNNDHV
                     IFRDKRVRQAMTYLTDRRQIVKSVLFGLGEVVDSHIYLFRPEYDKTLVSYPFDPRKAI
                     ELLNQTGWRDSDGDGILDKEIDGKRIPLRFEIKVNSGNAVRKSVALVLLDELKKHGIA
                     ASVRELDWTIFLNDVKNHNFDSVVLGWAMSVTEPDAYQVWHSSQAENKGSNAISYKNS
                     RVDAILESYRREFDPQKRIEMYKEFQQILNDEQPYTFLYIGKRVSVVQRRFAGVQVYP
                     AGLRPTEWWVPTGRQKYVSTLAAQ"
     gene            4712..5743
                     /locus_tag="VMZ02_01040"
     CDS             4712..5743
                     /locus_tag="VMZ02_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70538.1"
                     /translation="MSAYFLQRLLLMIPTFLGITLIVFLIVRLAPGDPAELRAAGGLG
                     AAAGAGISVEKKGAVDEAMAQWRAQYGLDKPLYMQYGIWLKNIFTLSFGDSFKDSQPV
                     WLKIAERLPVTLALNFWSLMVVYLVAIPLGIYSATHQNSLADQTTTLVAFVLFAVPLV
                     WAATMALVFLCGGDFFYLFPPGGLESLDAAENWSWWRKFTDQAWHLFLPVVLLSYDGF
                     AGLSRYMRSSMLEVLRQDYVQVARAKGLEERVVVFKHVLRNSLIPQVTLLASILPGLI
                     GGSVIIETIFSIPGLGQLGFEAVLSRDYPVVLALLAVTSILTLIGILISDLLLAAVDP
                     RIAFGRRAQ"
BASE COUNT         1303 a         1551 c         1595 g         1315 t
ORIGIN      
        1 cgtgtgctgc actgtggtga acttctgcaa cggcggcgcg ccaactttga gtgtcggcga
       61 atccggcaga acaagatccg gaaactcgtt ttccaactcg agcagacggc gaaacagccg
      121 gtcgtactcg gcatcgctta ccaggggatt gtcgagcacg tggtattgat agttgtgtcg
      181 ttcgatttcc tggcgcagtt cgacaacttc tttagccgca tgatcgtggg agggggtggc
      241 catcgacttt gttactcgct gaatttatga gtgaaaaagt tagcatagtt gtcgcggcga
      301 atgaagccat cggtcgcttc acattgaccc cctctggtcg gcatgttaaa cgttgccgtc
      361 atgggtacac gtggcgttcg ggcagctttt ttgatggcta ttctgtccgg ttgcgcgacg
      421 gcggtgccca atggtcaact aaccccggac gcgctgatct ggtctcccga gggacctttt
      481 agcaagactg ccaaagtcga cattcccgac gaagccggtg ccttcaatca ttttttaaaa
      541 ggccagttac tgctcatcga tggggagttc gagcccgctc tgaaagaatt tgaagccgcc
      601 tcgcaggcaa gtccgacgga cggcttttta cgttaccgct tggcgcagct ctatctacgc
      661 cgcggcgatt taaagagagc tatgggcgag gcggagtctg ccgccaaact ggaaccaaac
      721 agcgtcgact atcatttgct cctagctggg ctttattcgg cggtgggcga caatcaaaga
      781 ggactggcgg aatacaatga ggtattgaag ctcgatgcga aaaaccctga ggcgctcctc
      841 tatgccgggg ccttgtacct gcaaacagag gacctcgcac gggctacgca acgtttagat
      901 gagttgctcc aactcgatga caattccgcc ctcgggcact attatctcgg ccgagtgcgc
      961 gccaaaagta aactctattt aggcgctgag cagagctttc gtaaggcttt agagctcaac
     1021 ccgaagtcgg aagcggtctt gatggacttg gctttagtct acgaactcga aggcaagacg
     1081 gaagacgcga ttcaaaccta tcagcggctg ctgcaagtca atccacaaca tgtatggtcg
     1141 cgccggcgcc tgggcgagct ctatgtcgga cagaacaagc tcgatgaagc gctgcgccag
     1201 ttcgagcgcc tggaaggggc ggaaaccgac ccgcgcgata cccgcataaa gattggcttg
     1261 atctctttcg agaagggcga ctacgaccgg gccgccactg aattcaacct ggttctcgcc
     1321 ggcgatcctg aaaacgaccg cgcgcgttat tatcttggcg ctacttatat cgaactcaaa
     1381 gccaatgata aggcattgga agcgttcgaa cgaattcccg agaaaagcga gtactacgcc
     1441 gatgcccggg tgcaggcagc gcaactctat gagcgcaagg agcaaacccc caaagccatc
     1501 gaaattttgc agacggtcat caaaaaactt aacgaccgca aagaaatcca tgcctattta
     1561 agcgtgctct atcgtaagac caaggacttt ccgcgagcga ttcagtcgat ggagcgggtt
     1621 gtcgctctcg accctaaaaa cgacgaggcg cacttccaac tcggcgcgct ctatgacgag
     1681 agcaagatca aagacaaatc catctccagc atgaaaaaag ccattgagct caatcctaag
     1741 aacgctcccg cgcttaacta cttgggttac acttgggcgg agatgggcgt gcagctcgac
     1801 gaagccgaag atttgatcgc gcgggcgctc aaaatcgcgc ccaacgacgg tttttacatc
     1861 gacagtttgg gttgggttta ctatcagaag ggcgactatc cgcgcgccgt cgagcaactt
     1921 gaacgtgccg tggaactgac agtagacgat ccgacgatca ttgaacatct cggcgatgcc
     1981 tacgagaagg ccggtcggat cgaccgcgcc gtcgcccgct atcgtgaatc gagcaaactt
     2041 tccaaggatc ccgagcagag caaacgcctg cgcgaaaaaa tccagcggct ggagaaaagg
     2101 atttgaccgt cccgtttcgc cgcctcagtt cggtttaccc gacggtcgcc aactttctct
     2161 tcgccgttct ggcacttgcc gttgtcggtt gtgcgaccac ggccaaaccc ccggcagtgt
     2221 cgcaacttcc tgtgccgcag tgggagtcgg acaagttgat cgagtcgatc gctcaacgtc
     2281 gtcaacagtt tcgctcggtc cgcgcattgg ctcgggtcaa ctacgtcggg cctgagggta
     2341 agcatggttt cgaagaggcc gtgatggttc agcggcctga tcgtctgcgt ttggagacac
     2401 tgaccatgct gggagcaatc ctcatcgtca cggccaacaa ccaggagatt attggctatc
     2461 acccccgcga aggggtgcta gtgcgcggcc gcagctcaaa ggaaaacctg cagcgcctta
     2521 cgcaaattcc gctggagctc gaagagatca ccgccctgct gctgggtctg ccgccggtag
     2581 agccgtcggc gtcgtggcgc caagaaggca actcgctgag ctattcgctc aacggtcgga
     2641 cgaaagacaa tctgaccttt gaatcgcaat tgccggtgcc gactcaatgg cagcgcttca
     2701 atggcaccgg cgcgctggaa ttaaccgcgc gcttctccga ctacaccaag acgccagccg
     2761 gtttgtttcc ctcgaagatc aacatagagg cgccgctcca aggtaagaag ttggaaattc
     2821 gcttccaaga gcccgagttt aacgcgatcc ttcccgccga atcgttcacc cagcagaagc
     2881 cggtccatgt tcaagaagtc cccatcgaga tgatcggtag ttgagcgatg tcttgcaaag
     2941 gctggcgatt gatcctcccg ctgctttgtt tcgtcggttg cggcgacaag accgaagtta
     3001 aaatcccaga cgttagccca gccgcgaatc aagaaatctc cggcaaaccg gtgaccggag
     3061 attggctggt aatgcatagt ctcagcgatc cggagcagct caaccctctg accagcaacg
     3121 attcagcatc gagcgagatt atcgagctga tcttcgaagg tctgttatct cgcgacccgc
     3181 gcaccttggc gttcaaacct gtaatcgccg aggcacgtcc ggaagtttct ccagaccacc
     3241 ttagctacac gttcaaaata cgtaaggatg cgcattttca agacggtcgg ccgctgaccg
     3301 gtgaagacat tttgttcagc gtgaaagcga taaaatgccc cttcgtcaat gcgccgtttc
     3361 tgcgtgtcta cttcaactct ctcatcgatg ccgaattgat cgatccctac acgattcgct
     3421 tccggaccaa ggagccttac tttctcaatg agagcgtgct cggcggtatc agcgcattgc
     3481 cgcggcacta ttacgatccc gagaatctgt tggcgcgagt ctcggtcaaa cagctggatc
     3541 aagatccgca aaagcttccg ccccaagtga aacaatttgg cgagcagttc aacaagaact
     3601 atagccgtaa tccgatgggg agcggaccat tcaagtttgg cagctggaag actggacgcg
     3661 aggtagaatt gatccgtgac gatcaatatt gggggcgcgg caaagccgat atcgatcagg
     3721 tctatatcga tcggctgaaa ttccgcatta tcaacaatcc ggatgcggcg ctgattcgac
     3781 taaaaagcgg tagtttggat tatatcgagg cattacagcc ggtgcagtat gtgcgcggca
     3841 cgggcagcca aagattcaag caagagtttc aaacctatga atattacgcg cccacttaca
     3901 cctacatcgg ctggaataac gaccatgtga tctttcgcga taaaagagtt cgccaagcga
     3961 tgacctattt gacggaccgc cgccagatcg tcaaatcggt gctctttggc ctgggggaag
     4021 tggtcgacag ccatatctac ttgttccggc ccgagtacga taagactctc gtcagttatc
     4081 cgttcgatcc aaggaaagcg atcgagctgc ttaatcaaac cggttggcgt gacagcgacg
     4141 gcgacgggat cctcgataag gaaatcgacg gcaagcgcat accattacgt tttgaaatca
     4201 aggtgaactc gggcaacgcc gtgcgcaaga gcgtcgctct ggtcctgctc gacgagctta
     4261 agaaacatgg catcgccgcc agcgtgcgcg aactggactg gacgatcttc ttaaatgacg
     4321 ttaaaaacca taacttcgat tcggttgtcc tcggctgggc aatgagcgtg accgagccgg
     4381 atgcctatca ggtttggcat tcctcccagg cggagaacaa gggttccaat gcgatcagct
     4441 acaagaattc gcgtgtcgac gcgatcttgg aatcgtatcg tcgcgagttc gatccccaaa
     4501 agcgcatcga aatgtacaaa gagtttcaac aaatcttgaa cgatgagcag ccctacacct
     4561 ttctttacat cggcaagcgg gtttcggtgg tgcagcggcg ttttgccggc gtccaagtat
     4621 atcccgccgg cctgcgaccg accgaatggt gggtgccgac gggccgacag aaatacgtca
     4681 gtactctggc cgcccaataa ttgccctgct catgagcgcg tactttcttc aacgcttgct
     4741 gctgatgatt ccgacgtttc ttgggatcac cttgattgtg tttttgattg tccgcttggc
     4801 gccaggcgat cccgcggaac tgcgcgcggc cggcggtttg ggcgctgcgg ccggcgccgg
     4861 catttccgtg gagaagaaag gcgccgtcga tgaagccatg gcgcaatggc gcgctcaata
     4921 cggcctcgat aagccgctat acatgcaata cggcatctgg ctaaagaata ttttcactct
     4981 cagctttggc gattcattca aggatagcca gccagtgtgg ctcaagatcg ccgagcgact
     5041 accggtgacg ctggcactta atttctggtc cctcatggtc gtttacctgg tggcgatacc
     5101 gctggggatt tattcagcga cacatcagaa ctcgttagct gatcagacga ctacgctcgt
     5161 tgcatttgtt ctttttgccg tgccgctggt gtgggcagcg accatggcgc tggtgtttct
     5221 ttgcggcggt gacttttttt atctgttccc gcccggtggt ttggaatctc tggatgcggc
     5281 ggaaaattgg tcctggtggc gcaagttcac ggaccaggcg tggcatctat ttttgccggt
     5341 ggtgctgctt tcgtacgacg gctttgctgg gttgtctcgt tatatgcgct cgagcatgct
     5401 tgaagtcttg cgccaagatt acgtgcaggt ggcgcgcgct aaagggctgg aagaacgcgt
     5461 cgtcgttttt aaacatgtcc tacgcaattc gctgataccg caagtaactt tgctggcttc
     5521 gattctgccc ggtttgattg gcggcagtgt gattatcgaa acgatctttt ccattcctgg
     5581 tttgggacag ctcggtttcg aggcggttct gtcccgcgac tatccggtgg tgctggcgct
     5641 gctcgctgta acttcgattt taactttgat cggcatcttg atatcggatc ttttgttggc
     5701 tgcggtggat ccgagaatcg ccttcggtcg tcgggcgcag tgaacataat taccagatga
     5761 catc
//
LOCUS       DATLCH010000038         6771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7518183, whole genome shotgun sequence.
ACCESSION   DATLCH010000038 DATLCH010000000
VERSION     DATLCH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7518183"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1041)
                     /locus_tag="VMZ02_01045"
     CDS             complement(28..1041)
                     /locus_tag="VMZ02_01045"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70539.1"
                     /translation="MLLRRSVIVFAFALIALGQFSIKPSVAQERFRVSYGGFNETASS
                     MWVGIERGIFKKYGIDAQMVQVRNGALSVATLVAKEVEAVWPAQSTILSTVAGGIKLT
                     CIGGPVNKIPRNLMVRKEVKSAEDLRGKTVGVQSIGGGLWLQTMIILDHLGVDPEKYG
                     IKVRVIGDEATLAQAMITNNIDFGVITYGLSETLYSQGFRSLVDAGDITAPYQGPEVC
                     GLKESVASRNEFYLRVTKGLAEAVAFILDDSNKNEVTKVLQRNLRLSRNDVIEGSYKV
                     LRKMTTLDMAPNVAAFKSVQRIVARINPKIGQVDLDQVIDASFVRNLESSGFLAELRK
                     KIR"
     gene            complement(1097..1837)
                     /locus_tag="VMZ02_01050"
     CDS             complement(1097..1837)
                     /locus_tag="VMZ02_01050"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70540.1"
                     /translation="MRYGWRARIGHIAPAILDTSGEEMRKLLPAGVLHVGLTISEPIQ
                     TLGADQAAAAFNRMVDAGKRLAAEKVDVLICGGAPVALSKGLAGDAELAELLRRETQL
                     PVVTANGVVVDGLRAMGAHAVIVVSPFIEARNLEIKSFLEASGFAVAAIKGLGLTKNI
                     DFASQSPDAAFALAYGQAKAHPEADAIYIACPRWPSVDIIAALEADTGKAVVAAPAAM
                     VWGALKRLRINDCQPGFGRLMESLRMAR"
     gene            complement(2049..2609)
                     /gene="pal"
                     /locus_tag="VMZ02_01055"
     CDS             complement(2049..2609)
                     /gene="pal"
                     /locus_tag="VMZ02_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HUR70541.1"
                     /translation="MREQKRILQKGLKLLAITFLVSCGNPVNPPPPKFTGENLAAEKP
                     KPAAPKPPPAPQPEPKTSLDELRTGQSAAASPLKDIAFNFDSAALSEDARSMLRTNAE
                     WLKANGTARVQIEGHCDERGAADYNMALGAKRAQAAMDYLATLGVAATRMSTISYGEE
                     IPVCKDKTESCWAQNRRARFVVTAAK"
     gene            complement(2774..3937)
                     /locus_tag="VMZ02_01060"
     CDS             complement(2774..3937)
                     /locus_tag="VMZ02_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70542.1"
                     /translation="MAEANLQLFLLGAEKLLRCRWDGRSENVEILNSALEGETVREVA
                     PDPFNPKKLYAATLTEIHISENNGESWQWLPSGGIDYRDIWTMAVHPTRANEIYVGTL
                     PAAVYVSDNGGRSFHELSSLRNLSDYNKWTFPPGAHVAHARSIALDARVPDEIVVGIE
                     EGGVARSRDRGMTWEDISGPASPTAFPKINDPAGIIPYEMGKHEEGRVYRDVHLVVRH
                     PTRLETLYASTGIGTYRSDDDGKTWGKCEYGLGRAYAIPMAIHPGMPERLFLGAAENG
                     PATWAGYRTVRAGPYNTVKFSRNATAETGGAKTQILRSDDGGKNWSFLRNGLPAGTEH
                     MTCGFATHPADPNSLCVGYTDGSVYSTQDAGLNWRRLILSMPKLYGLRLIPAV"
     gene            4073..4948
                     /locus_tag="VMZ02_01065"
     CDS             4073..4948
                     /locus_tag="VMZ02_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70543.1"
                     /translation="MRPVKPPNPNPHAACFKLPPGACDTHLHVYGPFDRYPLVAERGY
                     DPDPHSTLDDYLRTHRALGLERAVIVTGSGNGTNNRITRDALKRMNGAFKGLALLDPA
                     ITDSELVELKESGFSGFRIKTNGRGGLSFDDSKRMVTRTAGFGWHVEFMSQSLAEAIG
                     AVPFLNSLKIPYMFDHVAHAEPHMTGEDHEFAELLRILKNEQQAWINLYSFYQLSKVG
                     PPDYQDMFEVVQAIVANRSDNIVWGSNWPHGGVSVPMPDDGDLLDFLFTAVPDENTRN
                     AILADNPAKLYGWLV"
     gene            5049..5774
                     /locus_tag="VMZ02_01070"
     CDS             5049..5774
                     /locus_tag="VMZ02_01070"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70544.1"
                     /translation="MYGWRARIGHIAPSRGDTLVYEFYKMFPEGFMMLNTTGTVRQLV
                     DSDFEAQLSRIEEAAQDLVDNNCDSIIFSGSPLFTRLPFGADRELGKKLSDKFGVPVA
                     AGLTAEVEALKAMNCTKLVVGTPYEDEINQRLKRHLEQCGFEVLQIAGYGVRKNAQLT
                     DLPVHASYKIAKRLYAKGRDADGIFIPCPRWPTITDVQLLEDEIGKPVITSSLACSWY
                     AMKMIDIREKVTGFGRLMESLAP"
     gene            5863..6348
                     /locus_tag="VMZ02_01075"
     CDS             5863..6348
                     /locus_tag="VMZ02_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70545.1"
                     /translation="MSWIKAAFLTGVLSSLMVNFVPDGMAAEWARGQGRGCSRGDRLN
                     IQDLDMSPDPLVEGQRVRAWKVRVDFTGGRDCETDIVVREGNKIVGHARNFRLHAGVN
                     EVEIPAVETYRYTGREHCFNVQVDLDGSRQQVDAARRFCARQRTMWSMREPDDRRYQN
                     R"
     gene            complement(6429..>6771)
                     /locus_tag="VMZ02_01080"
     CDS             complement(6429..>6771)
                     /locus_tag="VMZ02_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUR70546.1"
                     /translation="AAANPRAIAGGSQLMAGLLLLPFTPLAPPAGSVTLFIAVNMIVF
                     ALLSSGVAFLLYFRLIADIGPTKALTVTFLMPAFTMIWAGLFLNEKITAVMIVGCGLI
                     LAGTALVVVRK"
BASE COUNT         1545 a         1939 c         1745 g         1542 t
ORIGIN      
        1 ttgttttctt aaaagcgtgc gaatgcgcta tctgattttc ttgcgcagct ctgccaaaaa
       61 tccgctcgat tcgagattac ggacgaagct tgcatcgata acctgatcga gatcgacctg
      121 gccgattttt gggttgatgc gtgcaacgat ccgctgaaca gatttgaaag cggcaacgtt
      181 gggggccatg tcgagcgtag tcattttacg caagactttg taggaacctt cgatgacatc
      241 gtttcggctc aggcgcagat tgcgctgcag cactttggtt acttcgttct tattactgtc
      301 gtcgaggatg aacgcaactg cctccgccaa tcctttggtt acgcgtaagt aaaactcatt
      361 gcggctggcg accgactcct tcagcccgca aacttctggg ccctggtaag gcgccgtaat
      421 gtcgccggca tcgaccaatg atcggaatcc ctgcgaatac aaagtttcgc ttaatccgta
      481 ggtgatcacc ccgaaatcga tgttgttggt gatcattgcc tgagcgagag tcgcttcatc
      541 gccgataacg cgtactttta tgccatactt ctccggatcg acaccgaggt gatccaggat
      601 aatcatcgtt tgcagccata atccgccgcc gatgctttgc accccgacag ttttaccgcg
      661 tagatcttcg gcacttttga cctctttgcg caccatgaga ttacgcggga tcttattcac
      721 cggaccgccg atgcacgtga gcttgatgcc gccggcaacc gtgctcaata tcgtcgactg
      781 cgccggccag acggcttcga cttctttcgc gaccaacgtc gcgacgctca gcgcgccgtt
      841 acgcacctga accatttgcg cgtcgatgcc gtatttcttg aatatgccgc gttcgatacc
      901 gacccacatc gaactcgcgg tctcgttgaa tccgccgtaa gaaacccgga agcgttcttg
      961 cgcaacactc ggtttgatgg agaattgacc gagtgcgatt agcgcgaacg caaaaacgat
     1021 cacggatcgt cgaagcaaca tggcagaccc tttcaccgcg aaattggatt caccgccgct
     1081 caacggacga gccgcttcag cgcgccatcc ttaaactttc cattagtcgg ccgaagccgg
     1141 gctgacaatc gttaatgcgc aaccttttca gtgcacccca aaccatcgca gccggcgcgg
     1201 cgacaaccgc cttgccggta tcagcctcca gcgctgcaat gatatcgacc gacggccagc
     1261 gcgggcaggc gatgtaaatc gcatcggctt ccgggtgtgc tttagcttgg ccataggcga
     1321 gcgcgaacgc ggcatcgggc gattgactgg cgaaatcgat gtttttggtg aggccaagtc
     1381 ctttgatcgc cgccaccgcg aaaccgctcg cttcgagaaa acttttaatc tccagattgc
     1441 gtgcctcaat aaacgggctg acgacgatca ctgcatgcgc gcccatagcg cgcaagccgt
     1501 cgactacaac tccattcgcc gtcacaaccg gcaactgagt ctcccgcctc aataattctg
     1561 ccagctcagc atctcccgca agccctttgg acagcgccac cggagcaccg ccgcagatta
     1621 gtacatcgac tttttccgca gccaagcgtt tgccggcatc gaccatacgg ttaaacgctg
     1681 ccgcagcttg atccgcaccg agagtttgga tcggctcgct gatcgttaga ccaacatgca
     1741 gcacaccggc aggaagaagc ttgcgcatct cctcgcccga cgtgtcgaga attgccggcg
     1801 caatatgacc gattcttgct cgccaaccgt agcgcattta cttcacctct gttccagagt
     1861 gcaatggccg ctcacgcact taccaagacg tgcgaaccta acaaaggctc gctttctatg
     1921 tcaatggatt gtgattggca attcagcaag ctgaatcgcg aggcaggatt gctgcgcgag
     1981 gtaaaaaaaa agcccatgtc ttaaagacat gggctttttg tcgaagtatt ttttctatca
     2041 atccaaaatt actttgcggc agtgacaacg aaccgggctc ggcgattctg cgcccagcaa
     2101 ctctccgtct tatccttaca aacggggatc tcttcgccgt agctaattgt cgacatccga
     2161 gtggcagcca cacctaacgt agccaaataa tccatcgctg cttgtgcgcg cttggcacca
     2221 agtgccatgt tgtagtcggc agcaccgcgt tcatcacagt gaccttcgat ttggacgcgc
     2281 gctgttccgt tggccttaag ccattcggca ttggttcgca acattgaccg tgcgtcttcc
     2341 gagagagccg cactgtcgaa gttgaaggcg atgtctttca gcgggctagc cgcggccgac
     2401 tgtccggtgc gtagttcgtc caagcttgtc tttggttccg gctggggtgc cggcggtggc
     2461 ttcggcgcgg ccggtttcgg cttttcagcg gcgaggttct ctccggtaaa tttcggcggc
     2521 ggcggattca ccggattccc acatgaaacc aggaaagtga tcgccaacaa tttcaatccc
     2581 ttttgcaaaa tacgcttttg ctccctcatc aaaaccacct ttccagcccg tcctggactg
     2641 tttaatgggc caattgtgaa gtattgcggc gaacctgtca atcaattcgc tcgacaaaac
     2701 agaattatat aaactaccca ataaaaccat aatacttcag aaatatccgt taattcagcg
     2761 tttccagcgc ctgctaaaca gccggtatca atcggaggcc ataaagcttc ggcattgaga
     2821 ggatcaaccg tcgccagttt aaccccgcgt cttgggtgga gtaaaccgat ccgtcggtgt
     2881 aaccgacgca caaactattg ggatcagctg gatgcgtggc aaagccgcag gtcatatgtt
     2941 ctgtgccggc aggtaggcca tttctgagaa aactccagtt tttcccgccg tcgtcgctac
     3001 gcaagatctg ggtcttggca ccgccggtct cggcagtggc gttgcgactg aacttaaccg
     3061 tgttgtacgg gccggcgcgt acggtacggt aaccagccca tgtggcaggg ccattttcgg
     3121 cggcgccgag aaacaacctc tccggcatcc ctggatgaat cgccattgga atcgcgtacg
     3181 cgcgccctag cccgtactcg catttacccc aggtttttcc gtcatcatcg ctacggtagg
     3241 tgccgatccc cgtgctggca tatagagttt ccagtcgcgt aggatggcgt actaccaagt
     3301 ggacatcccg gtacactcga ccttcttcat gtttgcccat ctcgtagggg atgattccgg
     3361 ctggatcatt gatcttcgga aatgccgtcg gactcgctgg tccgctgata tcttcccaag
     3421 tcatgccccg gtcgcggctg cgcgccacgc cgccctcctc gatgccgacg acaatctcgt
     3481 ccggcacgcg cgcatcgagt gcaatcgaac gtgcatgcgc cacatgagcg cccgggggaa
     3541 aagtccattt gttatagtcg gaaagattac gcaacgacga taattcatga aaagatcgcc
     3601 cgccattgtc gctgacatag actgccgcgg gcaatgtgcc gacgtagatt tcattggccc
     3661 gagtcggatg caccgccatc gtccaaatat ctcgatagtc gattcctccc gaaggcagcc
     3721 actgccagct ctcgccatta ttttcactga tatgaatttc ggtcagcgta gcggcgtaaa
     3781 gttttttcgg attgaacgga tctggcgcga cttcacggac ggtttcgcct tcgagggcgc
     3841 tgttgaggat ctcgacgttt tcactccgac catcccaacg gcagcgcagc agtttttccg
     3901 caccgagcaa gaacaattgc agatttgctt ctgccataac ttacctccgc aactttaatc
     3961 ttggtttttg attgaacaag ccgtacgcta agcaaaacac ccgatgccgt caagcaccga
     4021 tcattgattc acgccgaata atcgtagcgc tttggaatag atcaatagca tcatgcgacc
     4081 cgttaaaccg cccaatccca atccacacgc agcttgcttc aaattaccgc caggcgcttg
     4141 cgacacacac ctgcacgtct atggtccgtt cgaccgttat ccgctcgttg ccgaacgcgg
     4201 ctacgatccc gatcctcatt caacactgga cgattactta cggacccatc gcgctctcgg
     4261 cttggagcgc gcagtcattg tcaccggcag tggtaacgga accaacaacc gcatcacccg
     4321 tgacgcgctc aagcgcatga acggcgcttt caaaggtctc gccttactcg acccggcaat
     4381 caccgattca gagcttgtag aattgaagga gagcggcttc agcggttttc gcatcaagac
     4441 gaacggccgc ggcggccttt cttttgatga cagcaagcgc atggttaccc gcacagcggg
     4501 attcggctgg catgtcgaat tcatgtcgca atcgctcgcg gaagcgatcg gcgcggtgcc
     4561 gtttttgaac tcccttaaga ttccttacat gttcgaccac gtcgcgcatg ccgagccgca
     4621 tatgaccggt gaggatcacg aatttgccga gctgctgcga attctcaaaa acgagcagca
     4681 agcctggatt aatctctaca gcttttacca gctgtcgaaa gtcggtccgc cggattacca
     4741 ggacatgttc gaggtcgttc aggcgatcgt cgccaatcgt tctgacaata tagtctgggg
     4801 cagcaattgg cctcacggtg gtgtgagcgt gcccatgccc gacgacggag acctgctcga
     4861 tttcctcttc accgcggtgc ccgatgaaaa tacccgtaac gcaattctcg cggacaatcc
     4921 ggcaaagctc tacggctggc tcgtatagac gaacgcaaca attgaattca ctgggatcga
     4981 tcgaatcggc atccaacgtt caaccttttg aacccttgga atctaaaaca actctgtagg
     5041 aggtaaaaat gtacggttgg cgcgctcgca tcggtcatat cgccccctca cgcggcgaca
     5101 ctctggtgta tgaattttac aagatgtttc ccgaaggttt tatgatgctc aatacgacgg
     5161 gcacggtgcg ccagctggtt gacagcgatt tcgaggcgca attaagccgt atcgaagaag
     5221 cagcccagga tttggtcgat aataactgcg actcgattat ctttagcggc tcaccgctat
     5281 tcacccgttt gccattcggc gccgatcggg agttgggcaa gaagctatcc gacaaattcg
     5341 gagtcccagt ggcggccggc ttgaccgcgg aagtcgaggc gttaaaagcg atgaattgca
     5401 cgaagctcgt cgtcggaacg ccctatgagg acgagatcaa tcaacggctt aagcgccatc
     5461 tcgaacagtg cggcttcgaa gtcttgcaaa tcgccggcta cggtgtccgg aaaaacgctc
     5521 agctcaccga cttgccggtg catgcatcgt acaaaatcgc caagcggctt tatgccaagg
     5581 gacgcgatgc cgacggcata tttatcccct gcccgcgctg gccgaccatc actgatgttc
     5641 agctcttgga agacgaaatt ggcaaaccag tgatcacaag tagcttggcg tgcagctggt
     5701 acgccatgaa aatgatcgat attagggaaa aagtgacagg atttggccga ttgatggaga
     5761 gtttggcgcc ctaagaaatt cgttaattac aaattcgcgg tgatacttta taattttttt
     5821 acgtctaaag aacaggttca gtcaaacaag gaggacataa aaatgtcgtg gattaaagca
     5881 gcatttttaa ccggtgtgct aagtagcctg atggtcaatt ttgtgccgga tggcatggcg
     5941 gccgagtggg cccgcggaca aggccgcggt tgttcgcggg gtgatcgctt gaacatacaa
     6001 gacctcgata tgtcgccgga cccgctcgtc gaaggccagc gggttcgcgc ttggaaagtg
     6061 cgcgtcgatt tcaccggtgg gcgcgattgc gaaaccgaca tagtagtccg cgagggtaac
     6121 aaaatcgtcg gtcatgcgcg caacttcaga ttgcacgctg gcgtcaatga ggtggaaatt
     6181 cccgccgtgg aaacataccg ttataccggt cgcgaacact gcttcaatgt tcaagtcgac
     6241 ctcgacggct cgcgccagca ggttgacgct gcgcgccgct tctgcgcccg ccagcggacc
     6301 atgtggagta tgcgcgaacc cgacgaccgc cgataccaaa atcggtagta gctaaataca
     6361 tcagaaaaca tctccggatg ggaatggcgc aaccgcgtcg ttcccacccg gttttttttc
     6421 tcctaccttc atttccgcac taccacaagt gcggtcccgg ccaggattaa accgcaccca
     6481 acgatcataa ccgccgtgat cttttcgtta agaaacaggc ctgcccagat catcgtgaaa
     6541 gccggcatca aaaaggtaac cgtcaatgcc ttcgtcgggc caatgtcggc gattaagcga
     6601 aaatacaaca ggaacgcgac gccgctgctc aataacgcga atacaatcat atttaccgca
     6661 ataaagagcg taaccgatcc cgctggcggc gccagcggcg tgaacggcag tagtaacagc
     6721 ccagccatca attggctgcc ccccgcaatt gcgcgtggat tggccgcggc g
//
LOCUS       DATLCH010000039         5241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7519992, whole genome shotgun sequence.
ACCESSION   DATLCH010000039 DATLCH010000000
VERSION     DATLCH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7519992"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VMZ02_01085"
     CDS             <1..376
                     /locus_tag="VMZ02_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HUR70547.1"
                     /translation="LGMSQTFIGIVFLAIVGGAAESGSAIAMARKNKLDLTVGIALGS
                     CIQIALFVAPALVLMSYALAPQPLELAFGRAEIGSLFLAVLIGAMVSGDGRSNWYKGV
                     QLITVYLIMAFLFYFMPEAVPQ"
     gene            373..1065
                     /locus_tag="VMZ02_01090"
     CDS             373..1065
                     /locus_tag="VMZ02_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HUR70548.1"
                     /translation="MSQGAAVGAGGLREWGRAYLRRRYAILFYTLLLTMVAAPVFAAF
                     EFKGFLIEFFLAANLLAAVMPVGAGRDRRILLAIVTLVWLARAATEWFDHPALSATVL
                     GMWTLIGLFAAAAALRFALCATAVDSEHLYAALSAYLLAGIFFGQFYWVLQQIWPESF
                     AVSGNFSRMSAFYFSFVTLATLGYGDIVPRSDVARGLAIVEGVGGQLFLAVMVARLVS
                     LYGGRSPGRNQS"
     gene            1238..3982
                     /locus_tag="VMZ02_01095"
     CDS             1238..3982
                     /locus_tag="VMZ02_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70549.1"
                     /translation="MATKIVDVTEQKRLNDAREKQIPWKKWGPYLSERQWGTVREDYS
                     ENGDAWNYFTHDHARARAYRWGEDGLAGISDDKQRLCFALALWNERDPILKERLFGLT
                     NGEGNHGEDVKEYYFYLDSTPTHSYMKYLYKYPQREYPYGDLIKTNRNRSREEFEYEL
                     LDTGIFADDRYFDVFVEYAKQDPEDVLIRITAHNRGPEAASVRLLPTIWFRNTWSWSD
                     DKRRPSLREAGPDTIEAIHPELGKYWLHCDGAAELLFTENESNRQRLWNQTNASPFVK
                     DAFHEYIIGGRREAVNPAKVGTKAAAQYVLNVPAGGSSAVRLRLVAAEKTNPFDSFDK
                     IFKSRIADADEFYERIAPQSLNEDQRRVHRQALAGMLWSKQYYFFDLDEWLEEHKSDR
                     LLESSRNVRNAEWFHMLNSDVISMPDKWEYPWYAAWDLAFHTISLSLVDFDFAKEQLL
                     LMLRNLYFHPNGQIPAYEWNFSDVNPPVHAWATLYLYRYESTLGRADLRFLERSFQGL
                     MLNFNWWVNRKDPSGRNVFAGGFLGLDNIGVFDRSAQLPTGGSLEQADGTAWMAFYCQ
                     SMLEIALILCEYDSMYEDIAFKFLQHFMWITYAMDRIGDNKDEMWDEQDGFFYDLLRF
                     PDGHAVRLKVRSLVGLLPLCASTVLEEDTATRYPRLMEMVALFRKRHPELISHIAPTD
                     AGFVGYKGRRLLSILNKKKLERVLAHMLDENEFLGPHGIRSLSRYHLEHPFVFTVAGH
                     EYNVRYLSAESNTGMFGGNSNWRGPVWMPVNILLIRALMNLYTFYADDFKVECPTRSG
                     RYLTLFEVAQELVRRLTGTFLRNDNGQRPVYGGTAKFQEDPHWRDLILFFEYFHGDNG
                     AGLGASHQTGWTGLVAPLLDIFGRVDANLVLETARGLVFSKLVTDQVGGEKQPDKARA
                     "
     gene            4401..>5241
                     /locus_tag="VMZ02_01100"
     CDS             4401..>5241
                     /locus_tag="VMZ02_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUR70550.1"
                     /translation="MLQRGKSIFAALALVFLVQACATVNWDYPRTPSNAFAHPETTSV
                     GALFQEAADKHPTLSGFSLVQHGENAFLARLAMADLAEKTLDAQYYIWDSDTTGRILA
                     SRLMRAADRGVRVRILIDDHYQTGDKDFLVAALDGHPNIEIRLFNPIRNRFWRTLSFL
                     ADFRRVNHRMHNKLFVVDNAVGMVGGRNIGDVYFGVRADHNYRDLDVLTSGPIVREIS
                     ASFDMFWNSDWAIPVGATVNELPTATEEKALLQRLEENVSAKGYPYPIYQSLDELRTR
                     LINI"
BASE COUNT         1109 a         1410 c         1534 g         1188 t
ORIGIN      
        1 actgggtatg tcgcagacct tcatcggcat cgtttttctg gccatcgtcg gcggcgccgc
       61 cgagagtgga tcggcgatcg ccatggcgcg aaaaaataag ctcgacctaa cggtgggcat
      121 cgccctcggc agttgtattc agatcgcgct gtttgttgct cctgctctgg tgttgatgag
      181 ttatgcgctg gcaccccaac cgcttgagct cgctttcggc agggcggaga ttggctcgct
      241 gttcctcgcc gttttgattg gcgcgatggt ttcgggggac gggcgatcga actggtataa
      301 gggcgtccaa ctcatcacgg tgtacctcat catggcattc ttattttact tcatgccgga
      361 ggcggtgccg caatgagcca gggcgccgcg gttggagcag gcggcctgcg cgagtggggg
      421 cgggcttact tgcgccgacg ctatgcgatt cttttctata cgcttttact gacgatggtg
      481 gccgctccgg ttttcgcggc tttcgagttc aaaggatttc taattgagtt ttttctcgcc
      541 gccaacttgc tcgctgcggt gatgcctgtc ggcgcgggga gagatcgccg tattttactc
      601 gcgattgtga cccttgtttg gctggcacgt gcggcgacag agtggtttga tcatccggcg
      661 ctctcggcga cggtactcgg gatgtggacg ctgatcgggc tgtttgccgc tgccgcggct
      721 ttgcgtttcg ccctatgtgc cacagcggtc gatagcgagc acctgtacgc ggcgctcagc
      781 gcctacctac tcgcagggat ttttttcggg cagttctact gggtgctcca acagatttgg
      841 ccggagtcct ttgcggttag cggcaacttc tcacgcatga gcgcgttcta ctttagcttc
      901 gtcacgctgg cgacgcttgg ctacggagac atcgtgccgc gcagcgatgt tgcgcgcggg
      961 cttgccatcg tcgaaggggt tggaggtcag ctattccttg ctgtaatggt tgctcgattg
     1021 gtgagcttgt atgggggaag atcgccggga agaaatcagt cctaacggtt aaagggcgaa
     1081 aaatgttttc gatgtccgcg ttagaattgc cggtggttaa aaattcgtaa accacggtcc
     1141 ataagcgctt ggaaagcccg agccaaatcg gttagtaagt cgaattcaca gcgccggtag
     1201 aacattggta aattaaggca gaggagactc cagggaaatg gctactaaga ttgtcgatgt
     1261 caccgagcaa aaacggctga acgatgcccg tgaaaagcaa ataccctgga agaaatgggg
     1321 tccttacttg agcgagcggc agtggggcac ggtgcgcgag gactatagcg aaaacggcga
     1381 tgcctggaac tattttaccc atgaccacgc gcgcgcgcga gcgtaccgct ggggcgaaga
     1441 cgggctcgcc ggcattagcg acgacaagca acggctgtgt tttgcgcttg cgctgtggaa
     1501 tgaacgcgac cccattctga aagagcgcct gttcggcctt accaacggtg aggggaatca
     1561 tggcgaggat gtcaaagaat attatttcta cctcgacagc acgccgaccc attcgtacat
     1621 gaagtatttg tacaagtatc cgcagcgcga gtatccctat ggcgatctga tcaaaaccaa
     1681 ccgcaaccgg tcgcgcgagg agttcgagta cgaactcctc gacaccggga tattcgctga
     1741 cgaccgctat ttcgacgtgt tcgtcgaata cgccaagcag gatcccgaag acgtgctcat
     1801 ccgtatcacg gcgcacaatc gcggaccgga agcggccagt gtgcgattgt tgccgaccat
     1861 ttggtttcgc aacacatggt cttggagcga tgacaagcgt aggccatcgt tgcgcgaagc
     1921 agggccggac acgattgagg caatacatcc tgaactcggc aaatactggc tgcattgcga
     1981 tggagctgct gaattacttt tcacggaaaa tgaaagcaac cggcagcgtc tgtggaatca
     2041 gaccaacgca tctcccttcg tcaaagatgc gttccatgag tacatcatcg ggggacgccg
     2101 tgaagcggtg aatcccgcca aggtcgggac caaggccgca gcccagtacg ttctcaatgt
     2161 gccggctggc ggcagcagcg cggtgcggct tcgtctggta gcggccgaga agactaatcc
     2221 cttcgacagc ttcgacaaaa tctttaagag ccgcattgcc gatgccgacg aattctacga
     2281 gcggattgcg ccgcaatctt taaacgagga tcaacggcgc gtgcatcgcc aggcgctggc
     2341 aggaatgctg tggagcaaac agtattattt tttcgacctc gacgaatggc tcgaggagca
     2401 caagagtgat cgactcctcg agtccagccg caatgtgcgc aacgccgaat ggtttcacat
     2461 gctcaacagc gatgtgatct ccatgccgga caaatgggag tacccgtggt atgcggcctg
     2521 ggacctcgct tttcatacga tatccctgtc gctggtggac ttcgatttcg ccaaagagca
     2581 gttgctgctg atgcttcgca atctttattt ccatcccaac ggacagattc ccgcatacga
     2641 gtggaacttc agcgatgtca atccgccggt gcacgcctgg gcgacacttt acctctacag
     2701 gtatgagagc accctcggcc gcgccgacct gcggtttctc gaacgttcgt ttcagggatt
     2761 gatgctgaac ttcaactggt gggttaatcg caaggaccca tccggtcgca acgtcttcgc
     2821 gggaggattt ctcggtctcg acaacatcgg cgtgtttgac cgtagcgctc agctgccgac
     2881 gggcggctcg ctggagcagg cggacggcac cgcttggatg gcgttttatt gccagagcat
     2941 gttggagata gctctgatac tgtgcgaata cgactcgatg tatgaggaca ttgcgttcaa
     3001 gttcctccag cactttatgt ggatcactta tgcgatggac cgcatcggcg acaataaaga
     3061 cgagatgtgg gatgagcagg atggcttctt ttacgatctg ctgcgatttc ccgatggtca
     3121 cgcggtgcgc ttgaaagtaa ggtcactggt cgggctgttg ccactgtgcg cgtcgacggt
     3181 gttggaggag gataccgcca ccaggtatcc ccggctaatg gagatggtcg ccctgtttcg
     3241 caagcgccat cccgagttga tctcgcatat cgcgccaacc gatgcggggt ttgtcggcta
     3301 caagggccgg cggctgttat cgatcctcaa taagaaaaag ctcgagcggg tgctcgccca
     3361 tatgctcgac gagaacgagt ttctcgggcc gcacggcata cgctcgttgt cgcgctatca
     3421 cttggagcat ccgtttgtgt ttaccgtcgc ggggcacgag tacaacgtcc ggtatctgtc
     3481 ggcggaatcc aataccggga tgttcggcgg caactccaac tggcgcggtc cggtgtggat
     3541 gccggtcaat attttgctga tacgtgcgtt gatgaaccta tacacgtttt acgccgatga
     3601 ctttaaggtt gaatgtccca cgcgctccgg ccgttacctg acgctgttcg aagtagcgca
     3661 ggagcttgtc cgccgtctga ccggcacttt tctacgcaac gataatgggc agcggccggt
     3721 ttacggcggc acggcgaaat tccaagaaga tccccattgg cgcgatctga ttctattctt
     3781 tgagtacttt cacggcgaca acggtgccgg cctgggcgcc agtcaccaga cgggttggac
     3841 aggccttgtc gcaccgttgc tcgatatttt cggccgcgtc gacgccaatc tcgtgctgga
     3901 gaccgcacgc gggctcgtct ttagtaaact cgtcaccgac caagtcggtg gagaaaagca
     3961 accggataag gcgcgagcgt agacgatgag ttagaacccc gcttcggccg gcgatgcggc
     4021 gcaggcgcgt ttggctagtg ccggaacatc ggcagtgaag aattgtccca cggcgacaat
     4081 gtctcggcta tcggtagacc gccagcgcca ttggactaaa gtccaatact agcgcctctc
     4141 agtcttttga tatgagccat aattatctct ccccacaagc ggaaccacgc gcggcatgcg
     4201 gcgcgagaat ttatgggtga gacagccggc aaattcaccg aaggaaagat tagattgcgg
     4261 aagatatttc tacgtttgca caaaacgccc ggcagtgatc aggcagcgcg tcatatctta
     4321 atttcgtaac tgccccatcc gctgccatag ggaattgtct cggtcgacta tcctcgagag
     4381 cacgagagaa actcaatctt atgcttcaac gaggtaaaag tattttcgca gctctcgcgc
     4441 tggtctttct ggttcaggca tgcgccacgg ttaattggga ttacccgcgt acgccgtcaa
     4501 atgcattcgc ccatcctgag accaccagcg tcggcgcgct ttttcaggag gcggccgata
     4561 aacatccgac cttatccggg ttctcgcttg ttcaacatgg ggagaacgct ttccttgccc
     4621 ggcttgctat ggccgacctg gcagaaaaaa ccctggacgc gcagtattac atctgggatt
     4681 cagacaccac cggccggatc ttagccagtc ggctaatgcg cgccgctgac cgcggcgtgc
     4741 gggtgcggat attgatcgac gaccattatc aaacaggcga taaagacttt ttggtcgcgg
     4801 cactggacgg ccatcccaac atcgagatac gactgttcaa cccgattagg aaccggtttt
     4861 ggcgcacgtt gagttttttg gccgatttcc gccgcgtcaa ccatcggatg cacaacaagc
     4921 tattcgtcgt ggacaacgct gtggggatgg tagggggacg caacatcggg gatgtttact
     4981 tcggcgttcg agcagaccac aactatcgcg acctcgacgt tctgacgagc ggtccgatcg
     5041 tccgcgagat atcggctagc ttcgatatgt tctggaacag cgattgggcc atccccgtcg
     5101 gtgcgacagt gaacgaactt ccgaccgcaa cagaagagaa agccttgctg cagcggctag
     5161 aggaaaacgt cagtgccaag ggttacccgt accccatcta ccaaagtctg gacgaactgc
     5221 gcacgcggct catcaacatt c
//
LOCUS       DATLCH010000040         7270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3054560, whole genome shotgun sequence.
ACCESSION   DATLCH010000040 DATLCH010000000
VERSION     DATLCH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054560"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /gene="rplQ"
                     /locus_tag="VMZ02_01105"
     CDS             complement(<1..280)
                     /gene="rplQ"
                     /locus_tag="VMZ02_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HUR70551.1"
                     /translation="MRHLKSGRQLSRNSSHRWALMRNLITALLREEKIKTTDPKAKEL
                     RRWVDRVITLGKRGDLHARRQAAAIIFDKTVVKKLFDTIGPRFKDRPGG"
     gene            complement(290..1312)
                     /locus_tag="VMZ02_01110"
     CDS             complement(290..1312)
                     /locus_tag="VMZ02_01110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HUR70552.1"
                     /translation="MSTMYKHWTELIKPKQLDVEERTLTATFGRFTAEPFERGFGQTI
                     GNSVRRILLSSLMGASIVAVRIKGVLHEFSTVAGVTEDVAEIILNLKEVRLRLNEGEQ
                     ATLKIDAKGPGIVSAKEISGSASVDILNPDHKIATLSRDGKLDMELLVKLGRGYVPAE
                     RNKEENAPVDTIFIDAIFSPVLKVNFTVSNARVGQRTDYDRLVFDVHTDGSVKPDDAM
                     AYAAKILQDQVSIFVNFQDEPRPEKRDDGPSIPLNENLFRSVDELEFSVRSQNCLQNA
                     DIKYIGELVQKSEQEMLRTKNFGHKSLNEIKEILREMGLELGMKVDHFPTRAEIEARK
                     REITKE"
     gene            complement(1334..1960)
                     /gene="rpsD"
                     /locus_tag="VMZ02_01115"
     CDS             complement(1334..1960)
                     /gene="rpsD"
                     /locus_tag="VMZ02_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HUR70553.1"
                     /translation="MARYIESVCRLCRRENLKLFLKGERCYTDKCAIERRTYPPGQHG
                     QGRPKFSEYSIQLREKQKVKRMYGLLENQFRRTFTEAARTKGITGETLLVLLERRLDN
                     VAYRMGFASSRAEARTLVRHGHILVNGKKVNIPSYTVRIGDVVSVKEPSRQITRVMSA
                     LEGAQRRGVPDWTEVDREAFSGRVKILPTRSDITMPINEKLIVELYSK"
     gene            complement(1981..2424)
                     /gene="rpsK"
                     /locus_tag="VMZ02_01120"
     CDS             complement(1981..2424)
                     /gene="rpsK"
                     /locus_tag="VMZ02_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HUR70554.1"
                     /translation="MAEAADEKKSEGSAGDAKAADKAAARKRRGRKNIAEGVVHIHST
                     FNNTIVTISDYQGNVISWSSAGAMGFKGSRKGTPFAAQQAADSAAKKAMDHGLRTVQI
                     YVRGPGAGRESALRALQSAGISISLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(2442..2825)
                     /gene="rpsM"
                     /locus_tag="VMZ02_01125"
     CDS             complement(2442..2825)
                     /gene="rpsM"
                     /locus_tag="VMZ02_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HUR70555.1"
                     /translation="MARIAGVDLPRAKRMEIALTYVYGIGRSTSRDILQAAGISLDLR
                     SDDLTDDDVAKIRQVLDQSYKVEGDLRREVTGNIKRHLDMGSYRGLRHRKNLPVRGQR
                     THTNARTRKGPRKTVAGKKTAPSKG"
     gene            complement(2899..3012)
                     /gene="rpmJ"
                     /locus_tag="VMZ02_01130"
     CDS             complement(2899..3012)
                     /gene="rpmJ"
                     /locus_tag="VMZ02_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HUR70556.1"
                     /translation="MKVRASVKKICNKCKVIRRKGVVRVLCENTKHKQRQG"
     gene            complement(3027..3245)
                     /gene="infA"
                     /locus_tag="VMZ02_01135"
     CDS             complement(3027..3245)
                     /gene="infA"
                     /locus_tag="VMZ02_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HUR70557.1"
                     /translation="MAKQDAIEVTGTVLETLPNAMFRVELENGHKVLAHISGKMRMHY
                     IKILPGDKVKIELSPYDLARGRIVFRER"
     gene            complement(3288..4046)
                     /gene="map"
                     /locus_tag="VMZ02_01140"
     CDS             complement(3288..4046)
                     /gene="map"
                     /locus_tag="VMZ02_01140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HUR70558.1"
                     /translation="MISLKSAREIEIMRRANVIVAEVLQELKRRVAPGVTTLELDAMA
                     EEMTLSRKAIPAFKGYNVAGRVYPRCLCASINDEIVHGIPSSRALREGDIVGLDYGVI
                     YEGFYGDSAMTVAVGNVSAAAQRLMDVTEASLRAGIEQLHEGKRLGDLGAAVQKVAED
                     AGYSVVRAFVGHGIGKKLHEEPPVPNYGERDRGLRLKEGMVLAVEPMVNVGSYEVEIK
                     EDGWTAVTKDGSLAAHFEHSIAITKNGPYILSQL"
     gene            complement(4043..4708)
                     /locus_tag="VMZ02_01145"
     CDS             complement(4043..4708)
                     /locus_tag="VMZ02_01145"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HUR70559.1"
                     /translation="MGGSARVILLGPPGAGKGTQAKLLEQEFGAVQISTGDILRKAVA
                     DQTPLGKQAAEFIKRGALVPDDLIIDLIAERLQQKDCAKGFVLDGFPRTIPQAESLDV
                     ILKKLGLTLNGVLSVQVPRKVIIERLAGRRTCKKCGAASHVVFESLKRPGVCDRCGGE
                     LFQREDDREETIANRLNVYEAQTAPLVDYYRSRGALREIDGVGSIPEIRARVLSALGG
                     LAS"
     gene            complement(4713..6023)
                     /gene="secY"
                     /locus_tag="VMZ02_01150"
     CDS             complement(4713..6023)
                     /gene="secY"
                     /locus_tag="VMZ02_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HUR70560.1"
                     /translation="MLSGFQNASRVPELRRRVIFALAMLAVYRLGVHVPTPGIDRAAM
                     AAFFDQQKNTLFGFLNLFSGGALEQFSVFSLGIMPYISASIILQLLTVVFPYLERLSK
                     EGEVGRRKITQYTRYGTVVLSIIQGTMIAIGLEGTQGPGGTPLVLNPGFAFRFMTVVT
                     LTSGTAFIMWLGEQITERGIGNGMSLIIFAGIVARLPSAIWSTFEFMRNGELGPLVAL
                     VMLVLSVAIIGAIIFVERGQRRIPVQYAKRVVGRRMYGGMTSHLPLKVNTANVIPIIF
                     ASSVLIFPATMAQFAKHPWIQTISGMLAPGQWLYEIAQISLIIFFAFFYTAVVFNPSD
                     VAENMKKAGGYIPGIRPGQKTAEFIEKVLSRITLSGAVYLCLIALLPSLLVRQLNVPF
                     YFGGTALLIVVGVALDTVAQMETHLISRNYEGFMKRGRLRGRKG"
     gene            complement(6016..6468)
                     /gene="rplO"
                     /locus_tag="VMZ02_01155"
     CDS             complement(6016..6468)
                     /gene="rplO"
                     /locus_tag="VMZ02_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HUR70561.1"
                     /translation="MKLEDLRPAAGSNKKRKRVGRGDGSGHGKTSCRGHKGQGSRSGG
                     NTKPGFEGGQMPLQRRLPKRGFHNIFRNEMAVVNLAQLDALAEVGDIAPETMSQHGLI
                     SGKNRQVKVLGEGNLTKAMTVRAHGFSSSAKQKIEAAGGKAELITLHA"
     gene            complement(6471..6968)
                     /gene="rpsE"
                     /locus_tag="VMZ02_01160"
     CDS             complement(6471..6968)
                     /gene="rpsE"
                     /locus_tag="VMZ02_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HUR70562.1"
                     /translation="MERIDPQGLELKEKVVHISRVAKVVKGGRRFSFSALVVTGDSHS
                     VVGYGMGKAKEVPEAIRKGLEQAKKNLIRVPIMDGTIPFEVIGHYGAGHVILKPASGG
                     TGVIAGGGVRAVVEASGIQNVLTKCLGSNNPHNMVKATFEALRDLRQPEEIAERRGKS
                     LAEIR"
     gene            complement(6993..>7270)
                     /gene="rplR"
                     /locus_tag="VMZ02_01165"
     CDS             complement(6993..>7270)
                     /gene="rplR"
                     /locus_tag="VMZ02_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HUR70563.1"
                     /translation="CVFRSNKHIYAQVISDSQGKTLATVSTLSEDLAEQLKKSKGVNA
                     AKQVGVALAKVCKENNISRVVFDRNGFIYHGRVKAVADGAREGGLDF"
BASE COUNT         1555 a         2140 c         1842 g         1733 t
ORIGIN      
        1 aaccgccggg tcgatccttg aaacgcggtc cgatggtatc gaaaagcttc ttgacgaccg
       61 tcttatcgaa gataatggcc gccgcctgac ggcgcgcatg caagtcgccg cgcttgccga
      121 gagtaatgac tcgatcgacc caacggcgca gttcttttgc cttaggatcg gtggttttaa
      181 tcttttcctc gcgcaagagt gccgtaatca gattgcgcat caacgcccaa cggtggctgg
      241 aattgcggct tagttgccgg ccggatttga gatgacgcat ggcttcaagc tactccttgg
      301 taatttcgcg cttgcgggct tcgatttccg cgcgagtcgg aaaatgatca actttcattc
      361 ccagctccag acccatctcg cgcagaattt ctttgatctc attcagagac ttgtggccga
      421 aatttttggt ccggagcatt tcctgctcac tcttttgcac cagctcaccg atatacttga
      481 tgtccgcgtt ctgtaggcag ttttgcgagc gcaccgaaaa ctcgagttca tcgacgctgc
      541 ggaacaggtt ctcgttgagc gggatcgacg gaccgtcgtc gcgtttctca ggccgcggct
      601 catcttggaa attgacgaaa atactcactt gatcctgcag aatcttcgcc gcatatgcca
      661 tggcatcgtc gggctttacg ctgccgtcgg tatggacatc gaataccaag cggtcgtagt
      721 cggtacgctg gccgacgcgc gcgttcgaca ctgtgaagtt gaccttcagc accggcgaaa
      781 aaatcgcatc gatgaaaatt gtatcgacgg gagcattctc ttccttgttg cgctctgccg
      841 gcacataacc tcgcccgagc ttgaccaata attccatatc gagcttaccg tcacgagata
      901 gcgtggcgat tttgtgatcg ggattgagaa tgtcgactga cgccgagccg cttatctcct
      961 tggcgctcac gatacccggc cctttagcat cgattttcaa ggtggcttgc tcgccttcgt
     1021 tcagccgcag gcgaacttct tttaagttta aaataatttc ggcaacgtct tcggtgaccc
     1081 cggccaccgt cgaaaactca tgtaaaacgc ctttgatacg gaccgccacg atggaggccc
     1141 ccattaatga cgataaaaga atccgccgca ccgagttacc gatggtttga ccgaaccccc
     1201 gttcgaacgg ttccgccgta aaacgtccaa acgtcgcggt cagagtccgc tcttcgacat
     1261 ccaactgttt gggtttgatc agctccgtcc aatgcttgta catcgtcgac atatcgtctc
     1321 ccaaattcga aacttatttc gaatacaatt ctacgatcag tttttcgttg atcggcatgg
     1381 tgatatcgct gcgcgtcggc aggattttca cgcgcccgct gaacgcttcg cggtcgacct
     1441 cggtccaatc gggcacaccg cgccgctgcg ccccttccaa agccgacatt acccgggtga
     1501 tctgtcggct tggttccttg acagaaacca catcgccgat gcgcaccgtg taggaaggga
     1561 tattcacctt cttgccgttc accaggatat gcccatggcg cactaaagta cgggcttcgg
     1621 ctctcgagct ggcaaaaccc atccgatagg caacattgtc gaggcgccgt tcaagaagta
     1681 cgagcaaggt ctcgccggtg atgcctttgg tgcgcgccgc ttcagtgaag gtgcggcgga
     1741 attgattttc caataggccg tacatgcgtt ttactttttg cttttcgcgc agctgaatgc
     1801 tgtattccga gaacttgggt cgtccctgcc catgttgccc tggcgggtag gtgcgccgct
     1861 cgatggcgca cttgtcggta taacaacgct cgcccttaag aaagagcttg agattttcgc
     1921 gccggcaaag gcggcacacc gattctatat atctagccaa acctgcttcc tcctctaaaa
     1981 tcagacccgc cggcgcttgg gcgggcggca accgttatgc ggaatcggcg taacgtcttt
     2041 gatcaagctg atactgatac ccgccgactg gagtgcccgc agtgccgatt cgcgtcctgc
     2101 gccgggccct cggacatata tttgtaccgt gcgtaagccg tgatccatgg ctttcttggc
     2161 ggcgctgtcg gctgcctgtt gcgcggcaaa tggagtgcct ttgcgtgagc ccttgaagcc
     2221 catcgcgccg gcgctcgacc atgagataac attgccctga taatccgaga tcgtcacgat
     2281 ggtgttattg aaagtggagt ggatatgcac cacgccctcg gcgatattct tgcgcccgcg
     2341 ccgcttgcgc gccgccgcct tgtcggcggc tttggcatcg cccgccgaac cttcgctctt
     2401 tttttcatcc gcagcttctg ccatggaaat ctcctagtta tttagccctt ggacggcgcg
     2461 gtttttttgc cggcgacggt tttgcgcgga cctttacgcg tccgtgcatt ggtatgagtg
     2521 cgctggccac gtaccggcaa gtttttgcgg tggcgcagac cacggtatga acccatgtcg
     2581 aggtgccgtt tgatgttgcc ggtcacttcg cggcgcagat caccttcgac tttgtaactt
     2641 tgatcgagca cctgacgaat tttagcgaca tcgtcgtcag tcagatcatc gctgcgcaaa
     2701 tccaaagaaa tcccggcagc ctgcagaata tcgcgcgacg tgctgcggcc aataccgtag
     2761 acgtaggtca atgcgatttc catgcgtttc gcccttggta agtcgactcc tgcgatacgt
     2821 gccatgtgtc actccgtcgc gcgcacgagc cgtagctggc gcggttgcaa tttagattct
     2881 aacccgctga aacctcagtt agccttgtcg ctgtttgtgc ttggtatttt cgcacagcac
     2941 ccgcaccacg cccttgcggc gaataacctt gcatttgttg caaatctttt tgactgacgc
     3001 tctaaccttc acgattgctc ctcgtattag cgctcgcgga atacgattcg tccgcgtgcc
     3061 aaatcatagg gagacagttc gatcttcact ttgtctccgg gcagaatttt tatgtagtgc
     3121 atgcgcattt ttcccgatat atgggccaat actttatggc cgttctcgag ctctacccgg
     3181 aacatcgcat tgggcagggt ctctaacacc gtaccggtga cttcaatggc gtcttgtttt
     3241 gccataacta aatcggggtt cctctgctgc cgcttctctc ctcagcgcta aagttgactc
     3301 aaaatgtagg ggccgttctt ggtaatcgct atagaatgct caaaatgggc ggctaagcta
     3361 ccgtctttgg tcactgccgt ccaaccgtcc tctttgattt cgacttcgta actgccgacg
     3421 ttcaccatcg gttcgaccgc cagcaccatg ccttccttga gtcgcagacc ccgatccctc
     3481 tcgccgtaat tcgggaccgg cggctcttcg tggagcttct tgcctatccc atggccaacg
     3541 aacgctcgga ccacggaata accggcatcc tctgcgactt tttgtactgc ggcgccgagg
     3601 tcgcctaagc gtttaccctc atggagctgc tcgataccgg cacgtaacga tgcttcggtc
     3661 acgtccatca atctttgcgc cgccgcactc acgttgccaa cggcaaccgt catggcggaa
     3721 tcgccataaa atccctcata gatcacgcca tagtcgagac cgacaatgtc accttcccgc
     3781 aaagcgcggc tcgatggtat gccatggacg atctcgtcat tgattgaagc acacaagcag
     3841 cgcggataaa cccgacccgc aacgttgtag cctttaaagg ccgggattgc ctttcggctg
     3901 agggtcatct cctcagccat ggcatccaac tccaacgttg tcactccagg agcgacgcgt
     3961 ctcttgagtt cctggaggac ctcggcaacg atgacgttcg ctctgcgcat gatctcgatt
     4021 tcgcgagccg acttaagcga aatcacgaag ccaagcctcc taaagcgctc aacacgcgag
     4081 cacggatctc ggggatgctg ccaacaccgt cgatctcccg taacgcccct cggctgcgat
     4141 aatagtccac cagcggcgcg gtttgcgcct catacacgtt caggcggttg gcgatggtct
     4201 cttcgcgatc atcctcgcgc tgaaacagct cgccgccgca gcgatcacaa actcccggcc
     4261 gttttagcga ttcaaaaacg acatggcttg cggcaccgca ttttttgcag gtgcgccgtc
     4321 cggctagcct ttctattatg actttcctcg gcacctgaac cgacagcacg ccgttgagag
     4381 tcaaacccaa ctttttcaga atgacgtcaa gactttccgc ctgagggatc gtccgcggga
     4441 agccgtcgag cacaaacccc ttggcgcaat ctttttgctg taatcgctcg gcgataagat
     4501 cgataatcaa atcgtcagga accaacgccc cgcgcttgat aaattccgcc gcttgcttcc
     4561 ccagcggcgt ttgatcggcg accgctttac gaaggatatc tcccgtggag atttgaaccg
     4621 cgccaaactc ctgctcaagc aacttggcct gagttccctt ccccgctccc ggagggccga
     4681 gtagaatcac gcgtgcgctg ccgcccaaga ctctagccct ttctgccgcg caacctacca
     4741 cgcttcatga acccttcata gttccggcta ataaggtggg tttccatctg cgcgacagtg
     4801 tccaatgcga ccccgacaac gatcagtaag gcggttccac caaaataaaa tggcacgttt
     4861 agttgccgca ccaacagcga cggcaacaag gcgatgagac acaagtacac cgcgccgctc
     4921 aaagtaatgc gcgacagcac tttctcgatg aactccgcgg ttttctggcc gggtcggatg
     4981 cccggaatgt aaccgcccgc ctttttcatg ttctcagcca cgtcgctcgg gttaaacacc
     5041 acggcggtgt aaaaaaacgc gaaaaaaatg atcagtgaaa tctgcgcgat ctcgtaaagc
     5101 cattggcctg gcgcgagcat tccggagatt gtttgaatcc aggggtgctt ggcgaactgc
     5161 gccatggtcg ccgggaaaat caacaccgag gacgcaaaga tgattggaat gacgtttgcc
     5221 gtattaactt tgagcggcag atgtgaggtc attccgccgt acattcgcct gccgactacg
     5281 cgtttggcgt attgcaccgg gatgcgccgc tggccgcgtt caacgaaaat gatcgcaccg
     5341 ataatcgcca cactgagaac caacatcacc agcgccacga gtgggccgag ttcgccattg
     5401 cgcatgaatt caaaggtaga ccagattgcc gaaggcagac gggcgacgat accggcaaaa
     5461 ataatcaacg acatgccgtt gccgatgccg cgttcggtga tctgctcgcc caaccacatg
     5521 ataaaggccg tgccggacgt tagcgtaacc accgtcataa agcggaaagc aaatccggga
     5581 ttcaatacca aaggcgttcc gccaggtccc tgcgtccctt ccaacccgat cgcgatcatt
     5641 gtcccttgga taatcgacaa gacgaccgtg ccgtagcgag tatactgggt aatcttacgc
     5701 cggccgactt ccccttcctt cgacagcctt tccaagtaag gaaacacaac cgtcagcaat
     5761 tgcaaaataa tggaagcgct gatgtaaggc ataatgccca gggaaaaaac tgagaattgc
     5821 tccagcgcgc cgccggagaa tagattgaga aagccgaaaa gagtattctt ctgctgatcg
     5881 aaaaacgccg ccatcgcggc gcgatcaatc ccaggtgtcg gtacatggac cccgagacga
     5941 taaacggcca gcatcgccag ggcgaaaatc acccggcgac gcagttctgg cacccgtgag
     6001 gcgttttgga aaccgctaag catgaagtgt tatcaactcc gctttcccgc cggccgcttc
     6061 gattttctgt ttggcgctgc tggaaaagcc atgtgcccgg acagtcattg ccttggtcaa
     6121 attaccctcg cctaaaactt ttacttgacg gtttttaccg ctaatcaggc catgttgaga
     6181 cattgtttct ggcgcgatgt cgccgacctc cgccagcgcg tccagttgcg ccaaattgac
     6241 cacggccatt tcgttgcgaa aaatattatg aaagccgcgc ttgggcaaac gtcgctgaag
     6301 cggcatctgg ccaccctcga agccaggttt ggtattccct ccggaacgcg agccttgacc
     6361 tttgtgtccg cggcaagaag tcttgccatg gccggaaccg tcgccccggc ctacacgttt
     6421 acgcttctta tttgaaccag cggcaggccg caaatcttcc aatttcatag ttagcgtatc
     6481 tccgctaaag atttaccgcg tcgttcggcg atttcttccg gctggcgtaa atcgcgcaga
     6541 gcttcgaatg tcgctttgac catgttatgg gggttattgg aacccagaca tttggtcagg
     6601 acgttttgga tgcccgaggc ctcgaccacc gcgcgtacac cgccgccggc gatcacgccg
     6661 gttccgcctg acgcgggttt gagaatgaca tggccagcac cgtagtgtcc gatcacttcg
     6721 aagggaatag tcccgtccat aatgggtact cggatcaaat tctttttcgc ctgctccaag
     6781 cctttgcgga tcgcttccgg aacttctttc gccttgccca ttccatagcc aacgacggag
     6841 tggctatcgc cagtgaccac cagagcactg aagctaaagc gccggccgcc tttaacaacc
     6901 ttggccaccc ggctgatgtg aacgactttt tctttcaact ctaaaccttg cggatcaatt
     6961 cgttccaaag cgacctctcg ttcaaatgac ggttaaaagt cgagtccacc ttcgcgagcg
     7021 ccatctgcca ctgccttgac tcggccgtga tagataaagc cgttacgatc gaaaacgacg
     7081 cggctaatat tgttttcttt gcaaaccttg gccaaagcga cgcccacctg cttggcggca
     7141 ttgacgcctt ttgatttctt aagctgctct gccaaatctt cggacaatgt agatactgta
     7201 gccaacgtct ttccttggct gtctgaaatg acctgcgcat agatatgttt attgctgcga
     7261 aatacgcaaa
//
LOCUS       DATLCH010000041         9911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9080467, whole genome shotgun sequence.
ACCESSION   DATLCH010000041 DATLCH010000000
VERSION     DATLCH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9080467"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VMZ02_01170"
     CDS             complement(<1..624)
                     /locus_tag="VMZ02_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUR70564.1"
                     /translation="MARTVSLEKTRNIGIMAHIDAGKTTTTERILYYTGINYKIGEVH
                     EGTATMDWMVQEQERGITITSAATTCFWRDHRVNIIDTPGHVDFTIEVERSLRVLDGA
                     VAVFCSVGGVEPQTETVWRQADKYGVPRLAFINKMDRVGADFFRVVKRIETRLGASPA
                     VMQLPIGAEDKFAGIVDLITMKAVVWEDESLGAKFHQEEIPADLKAQA"
     gene            complement(694..1164)
                     /gene="rpsG"
                     /locus_tag="VMZ02_01175"
     CDS             complement(694..1164)
                     /gene="rpsG"
                     /locus_tag="VMZ02_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HUR70565.1"
                     /translation="MPRKGEVRKREILPDPKYGDKVVAKFVNTIMNQGKKSVAESILY
                     HSLDIITAKTKEDSLGIFKRAIDNTRPTVEVRSRRVGGATYQVPVEVRPQRRLSLSMR
                     WLIDAARDRAEKSMEEKLAAELIDAANNRGTAIKKKEDTHRMAEANRAFAHYRW"
     gene            complement(1170..1541)
                     /gene="rpsL"
                     /locus_tag="VMZ02_01180"
     CDS             complement(1170..1541)
                     /gene="rpsL"
                     /locus_tag="VMZ02_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HUR70566.1"
                     /translation="MPTISQLVRESREKQRRKNTAPALQSCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGVEVTSYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGTL
                     DSIGVQDRRQSRSKYGAKKPK"
     gene            complement(1646..5773)
                     /gene="rpoC"
                     /locus_tag="VMZ02_01185"
     CDS             complement(1646..5773)
                     /gene="rpoC"
                     /locus_tag="VMZ02_01185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HUR70567.1"
                     /translation="MEDLFNLFEKPKNPLSFNAIKVSVASPEKIRSWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPTKDYECNCGKYKRMRHRGVVCEKCGVEVIQSKVRRERM
                     GHIELATPVVHIWFLKSLPSRIGALLDMSLKDIEKVLYFESYIVVDPGETPLQYKELL
                     TEARYRKAVEEYGGKFVAGMGAEAVRKLLKEIDIAKVAEELRQEMRDANSEVRRKKMA
                     KRLKVLSAFRTSGNRPEWMVMEVIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLMELSAPDMIIRNEKRMLQEAVDALFDNGRRGRAITGQNRRPLKSLSEMLKGK
                     TGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKKMALELFKPFIYNKLEERGHVT
                     TIKSAKKMVERELPEVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPILIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLSVEAQVEARALMMSTNNILSPAHGKPIIVPTQDIVL
                     GLYYMTRDRLHAKGSGRLFSSPTEVRVAYDQGEVDLQAPITVRIHGERVETTTGRVLL
                     YDVVPEVIPFKEVNRVMKKKELANLIDVSFRLAGNKATVILADRLKDVGYRHATKAGI
                     SISIKDMLIPPTKEAMLKKAHDDVREIEEQYKNGLITDGERYNKVVDIWAEVTDRIAD
                     QMLGDLGTEKVTDEQGHEFTVPSFNPIFMMADSGARGSAQQIRQLAGMRGLMAKPSGE
                     IIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDAVIGE
                     EDCGTMDGIEITPLMEGGEVIEPLGDRVLGRVALEDVVDPFTGEVIVKANQEINEELV
                     QRVEDAGLERIKIRSVLTCASRRGVCIMCYGRDLGRGHMINMGEAVGVIAAQSIGEPG
                     TQLTMRTFHIGGTASRRAEQTTLEARNDGNLKFINVSTVTNKEGDFVVMNRNGEVAIV
                     DYPEGEKGRVRERERYPVVYGAKLKKKDGAKVKAGELLAEWDPYTIPILTEVSGPVKF
                     GDMIENVTMEEKVDERTGLSTKVIIDSKDIDKRPRISIKDTQGQTTRLTATAEARYLI
                     PVGAHINVQDGQEVFAGDILAKIPRETTKTKDITGGLPRVAELFEARKPKEFAVISEI
                     DGAVSFGKDTKGKRKVVVTPEVGEPREYLIPKGKHISVHDGDRIRAGEALMDGSSNPH
                     DILNILGEKALAKYLVDEVQEIYRLQGVRINDKHIEVIVRQMLRRVRIKDVGDADFLV
                     GDQIEKWRFEDENQRVIGENGKPAVAEPLLLGITKASLSTESFISAASFQETTKVLTE
                     AAIQGKIDLLRGLKENVIMGRLIPAGTGVTNYAKAEIETDEIEDGAAPMDHAVEA"
     gene            complement(5819..>9911)
                     /gene="rpoB"
                     /locus_tag="VMZ02_01190"
     CDS             complement(5819..>9911)
                     /gene="rpoB"
                     /locus_tag="VMZ02_01190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HUR70568.1"
                     /translation="VANNLRFRRSFGRIRKIIDLPYLIEIQKNSYELFLQKDAAPNER
                     QNMGLQEVFKSVFPIKDFNETASLEFVSYTLGEPKYDVEECHQRGMTYAAPLKVTVQL
                     VLWDVDQQTGARSIKNVKEQEVYFGEVPLMTINGTFMINGTERVIVSQLHRSPGVFFE
                     HDKGKTHASGKLLYSARVIPYRGSWVDLEFDPRDVVYVRIDRRRKFHATVLLRALGMT
                     SEDLLNYYYRSDTIHFDGRKVTVEFNANHLLGAKVSADVRDPKSSDIIVKEGKKFTRP
                     VLRQMEAARLKQVPIALEEILGRVAAHDIVDPHGKEVLVECNHEITQEKLDLIRDKGL
                     TQIDVLFIDESSVGPYLRNTLLQDRIRSPQDAILEIYRRLRPGDPPTVESATNFFNNL
                     FFNPDRYDLSKVGRLKLNHRLKLNIPLEQGTLRKEDILEVVRYLMELKNGNGSVDDID
                     HLGNRRVRAVGELVENHFRVGLVRMERAIKEKMSLQDIETLMPQELINYKPVSAALKE
                     FFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRVCPIE
                     TPEGPNIGLIASLTTYARVNDFGFIETPYREVENGRVTDRIRYLSALEEEDHVIAQAN
                     APIDGRGNFSADLVSSRKGGEFVMARPEEVNFMDVSPNQLVSVAASLIPFLENDDANR
                     ALMGSNMQRQAVPLLRTEAPFVGTGMERTVARDSGVTIVARRDGLVESVDSTRIVVKA
                     DKPAGSRDTGVDIYNLVKYQRSNQNTCNNQRPIIVVGDQVKAGDVIADGPSTEMGELA
                     LGRNVVVALMPWGGYNFEDSILINERVVKEDIYTSIHIEEFECVSRDTKLGPEEITRD
                     IPNVGDEALTDLDESGIIRIGAEVKPGHILVGKITPKGETQLSPEEKLLRAIFGEKAG
                     EVRDTSLRVPPGVEGTVINVRIFSRKGLGKDDRSKSIEDEEVTRLRKDQHDEVRILQE
                     GALRRLKKLLVGRRLASRISDDSRKVLLNKGQEMTEEDLDQLPMSYWSEIQVDNESVE
                     NELGRIIESTTTQVNLIKHAFEDKIEKLKGGDELPPGVIKMVKVFVAIKRKLQVGDKM
                     AGRHGNKGVISRILPEEDMPYLEDGTPVDVVLNPLGVPSRMNVGQILEAHLGWAARSL
                     GHQIDDLLRTHNGDIDQLRRRLKDYLNTRDANELIDGAKDVEVLKLASKYRDGVRIAS
                     PVFDGAAEEEIFNLLRKAGLPATGQATLYDGRTGEAFDGKVTVGVMYIMKLHHLVDDK
                     IHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGAAYTLQEMLTVKSDDVIG
                     RTRMYEAIVKGDNLLEPGLPESFNVMVKELQSLGLDVDLVEEK"
BASE COUNT         2137 a         2821 c         2508 g         2445 t
ORIGIN      
        1 ggcttgggct tttagatctg ccgggatttc ttcttgatga aacttggcgc ccaagctctc
       61 atcttcccaa accaccgctt tcattgtgat cagatcgacg ataccggcga atttgtcttc
      121 ggcaccgatc ggaagctgca tgaccgccgg actagccccg agccgggttt caatcctttt
      181 gacgactcgg aagaagtcgg cgccgacgcg atccatctta ttgataaagg cgaggcgagg
      241 aactccatac ttatcggcct gacgccaaac cgtttcagtt tgaggttcga caccgccgac
      301 cgagcaaaat accgccactg cgccgtcaag cacgcgtaaa gaacgctcga cttcaatggt
      361 gaaatcgaca tggccgggag tatcgataat attgacccgg tggtcacgcc agaagcaggt
      421 ggtcgccgcg gatgtgatcg tgataccgcg ctcttgttcc tggaccatcc agtccatcgt
      481 tgcggtaccc tcgtgcactt cgccgatctt ataattgatg ccggtgtaat agaggatccg
      541 ctcggtggtc gtggtcttgc cggcatcgat atgcgccatg atgccgatgt tgcgggtctt
      601 ctctaatgag acagttctag ccattttctt tacgccggaa aaaaaggagg cggtcaaacc
      661 gcctcctttt gcgaactcaa atttgaaggt tacttaccaa cgatagtgag caaaggcccg
      721 gttggcctcc gccatacgat gcgtgtcttc ttttttctta atcgctgtgc cgcgattgtt
      781 ggcagcgtcg atcaattccg ccgccaattt ttcttccata gacttctcgg cacggtcccg
      841 cgccgcatcg attaaccaac gcatgctcag cgacaatcgc cgttgcggcc gtacttccac
      901 ggggacctga taagtagcgc caccgacacg ccgagaacga acctcaacgg tgggccgggt
      961 attatcaatg gcacgcttga aaatccccaa ggaatcttcc ttggtcttag cggtgatgat
     1021 atcgaggcta tgatagagga tcgattccgc tacgctcttc ttcccctgat tcatgatcgt
     1081 gttgacgaac ttcgccacga ccttgtcgcc gtactttggg tccggcagaa tctctcgttt
     1141 tcttacttca cctttgcgcg gcataaatat tacttcggtt ttttagcgcc atatttagag
     1201 cggctctggc gacgatcttg aacgccgata gaatcaagcg tgccgcggac gatgtgataa
     1261 cgaactccag gaagatcttt tacacggcca ccgcgaatca aaacgacgga atgctcctgc
     1321 aaattgtgac caacaccggg aatataagac gtcacttcga caccattggt caaccgtaca
     1381 cgagcgacct tgcggagcgc cgagttgggt tttttcggag tcgaagtgta aacacgcgtg
     1441 cacacaccgc gcttttgcgg acagctctgc agcgcagggg cagtattttt tcggcgctgc
     1501 ttttcgcggc tttcgcgcac gagttgacta atagtcggca taggtaatgt ctcgtctaat
     1561 ctcgttgaag ttttgggagc aaattgaacg ctaaactccc cgggaaaatc ctttgtttaa
     1621 caaactaatc cgctattgtc aaagcttacg cttccaccgc atggtccatg ggtgctgcac
     1681 catcttcaat ttcatcggtc tcgatctcgg ctttggcata attggtgaca ccggtgccag
     1741 ccgggataag tcgacccata atgacgttct ccttcaatcc gcgcagcaaa tcgattttgc
     1801 cctggatagc cgcttcggtg agcaccttgg tggtctcttg aaaagacgcg gcggagataa
     1861 agctttcggt cgacaacgat gctttggtaa ttcccaataa caggggctcg gccaccgcag
     1921 gcttgccatt ctcgccgatg acccgctggt tttcatcttc aaaacgccac ttctcgattt
     1981 gatcgccaac caagaaatcg gcatcgccga cgtctttgat gcgcacccgg cgcagcatct
     2041 gacgcacgat aacctcgata tgcttgtcgt tgatgcgcac accttgcaaa cgataaatct
     2101 cctgcacctc gtcgacgaga tatttggcca gagccttttc gccgagaatg ttgagaatat
     2161 cgtgcgggtt ggatgagcca tccatcaacg cctcgccggc gcggatgcga tcgccgtcat
     2221 ggacgctgat atgtttgccc ttagggatca agtactcgcg cggttcgccg acctccggcg
     2281 taaccacgac tttacgtttc cctttagtgt ccttaccgaa ggaaacggcg ccgtcgattt
     2341 cgctgatgac ggcgaattct ttcggcttgc gggcttcgaa aagctcggca acacgaggta
     2401 gaccacccgt gatatcctta gtcttcgtgg tttcccgcgg gatctttgcc aaaatatcgc
     2461 cggcgaatac ttcctggccg tcttggacgt tgatatgcgc tcccaccgga atcaaatagc
     2521 gcgcttccgc agtggcagtc agccgagtcg tctgaccttg agtgtccttg atggaaatgc
     2581 gcggccgttt gtcgatgtct ttcgaatcga tgattacctt ggtcgacaga ccagtgcgtt
     2641 catcgacttt ctcttccatc gtgacgtttt cgatcatgtc gccgaatttg acaggtccgc
     2701 taacctcggt gagaattggg attgtgtacg ggtcccattc agccagtagt tcgcccgctt
     2761 tgacttttgc gccgtctttc ttcttgagct tggcgccata taccacggga tagcgttcac
     2821 gttcgcgcac acgtcccttc tcgccttctg gataatctac aatcgccact tcaccgttac
     2881 ggttcatcac gacgaaatcg ccttctttgt tggtgacggt gctgacattg ataaatttca
     2941 gattaccgtc gttgcgcgcc tccagcgtgg tctgctccgc gcgtcggctg gcggtgccgc
     3001 cgatatggaa cgtacgcatc gttagctgcg tgcccggttc accgatggac tgcgctgcga
     3061 tcacgccaac tgcctcgccc atattgatca tatggccacg ccccaagtca cgtccgtaac
     3121 acatgatgca aacaccgcgg cgcgacgcgc aggtcaatac cgaacgaatc ttgattcttt
     3181 ccaggccggc atcttcgacc cgctgcacta attcttcgtt gatttcttgg ttcgctttga
     3241 cgattacttc acctgtaaaa ggatcaacca catcttccag cgccacccgg ccaagcacgc
     3301 gatcaccgag cggttcgatg acctcgccgc cttccatcaa tggcgtgatc tcgatgccgt
     3361 ccatggtccc gcaatcttct tcgccaataa cagcgtcctg cgcgacgtct accagacggc
     3421 gcgtcaggta tccggaattg gcggttttta aggcagtatc ggccaacccc ttgcgggcac
     3481 cgtgggttga gatgaagtac tgcaaaacgg tcaatccttc gcggaaattg gccgtgatcg
     3541 gcgtctcgat aatttcgcct gagggcttcg ccatcaaacc gcgcatgccg gctaactggc
     3601 gaatctgttg cgcgctgccg cgagcgccag agtccgccat catgaaaatc gggttgaagc
     3661 tcggcaccgt aaactcgtga ccctgctcat cggtaacctt ctccgtaccc aaatcgccga
     3721 gcatttgatc agcgatgcgg tcggtgactt ccgcccaaat gtcgacgact ttgttgtaac
     3781 gttcaccgtc ggtgatcaga ccgtttttgt attgctcttc gatttcacgc acgtcgtcat
     3841 gcgccttctt gagcatcgcc tctttggtcg gcggaatcag catgtctttg atcgagatcg
     3901 aaatgccggc cttagtggca tggcgatagc cgacgtcttt taaccgatcg gcaagaataa
     3961 ccgttgcctt gttaccggca aggcggaagc tcacgtcgat caagttggca agctcttttt
     4021 tcttcattac ccggttgact tccttgaaag gaatcacctc ggggaccaca tcataaagca
     4081 gcacacgccc ggtcgtggtc tcgacgcgtt cgccatggat acgcacggtg atcggcgctt
     4141 gcagatcaac ctcaccctgg tcataggcca cccggacttc ggtcggactg ctgaacaatc
     4201 gaccgctccc cttggcatgc aagcggtcgc gcgtcatgta atacaagcct aaaacaatat
     4261 cctgggtcgg cacgatgatc ggcttaccgt gagctggcga caagatattg ttagtggaca
     4321 tcatcaaggc gcgcgcctcg acctgggctt caacggacaa aggcacgtgg acggccatct
     4381 gatcaccgtc aaaatccgcg ttgtaggcgg cgcaaaccaa tggatgaagt tgaatcgctt
     4441 tgccttcaat aaggatcggt tcgaacgcct gaatccctaa acgatgcagc gtcggcgccc
     4501 gattgagcag cacgggatgc tcgcgaatca cttcatcgag aatgtcccaa acttccggga
     4561 gctcgcgctc caccattttt ttggcgctct tgatcgtcgt tacatggccg cgctcttcga
     4621 gcttgttgta aataaacggc ttgaatagct cgagggccat ttttttcggc aacccacact
     4681 gatgcaagcg caactctgga ccaacgacga tgaccgaacg ccccgaatag tcgacgcgct
     4741 taccgagcaa gttttggcgg aaacggccgg ttttgccttt tagcatttcg ctcagggatt
     4801 tgagcggccg gcggttttgg ccggtaatcg cccgaccgcg tcgcccgttg tcgaacaagg
     4861 catcgaccgc ttcttgaagc atgcgctttt cgttgcggat gatcatgtcc ggcgcactca
     4921 gctccatcag gcgtttgagt cgattgttgc gattaatgac gcgccgatac agatcgttta
     4981 agtcggatgt ggcaaatcgt ccgccatcga gaggcaccag cggacgcaag tccggcggaa
     5041 tcaccggaat cacttccatg accatccatt ccggacgatt acccgaagtc cggaaagcac
     5101 tcaaaacttt aaggcgcttc gccattttct tgcgccgcac ctcggaattg gcatcgcgca
     5161 tttcttggcg caattcctcg gcaaccttgg cgatgtcgat ctctttgagc aacttgcgca
     5221 ccgcctcggc acccattccc gcaacaaact tgccgccgta ttcttcgacc gccttacggt
     5281 atcgcgcttc ggtcagcaac tctttgtact gcagaggcgt ctcgccggga tcgacgacaa
     5341 tataagattc gaagtagagc actttctcga tgtctttaag ggacatgtcg agcagggcac
     5401 caatccggct gggcaggctt ttcaaaaacc aaatatgcac cacgggagtc gctagttcga
     5461 tatgtcccat gcgctcgcgg cgcactttag attgtataac ttcgacaccg cacttctcgc
     5521 agactacgcc gcgatggcgc atacgtttgt acttgccgca gttgcattcg taatcttttg
     5581 tcggcccaaa gatctttgcg caaaacaagc cgtcacgttc cggcttgaac gttcggtaat
     5641 taatcgtctc gggcttttta acttcaccat gagaccatga gcgaatcttt tccggcgaag
     5701 caacggaaac tttgatggca ttaaaactga gcggattttt cggcttttca aataagttaa
     5761 acagatcttc cataacagga tcacctccgg tgttagcggc acaacgtact ggggttacct
     5821 acttttcttc gacgagatcg acgtccaagc ccaaactttg gagctctttc accatgacgt
     5881 taaaagattc aggaagtccc ggttcgagca gattgtcacc cttgacgatc gcttcataca
     5941 tgcgcgtgcg cccgatgaca tcgtcggact tcaccgtaag catttcttgc aacgtgtatg
     6001 cggcgccata agcttccagg gcccagactt ccatttcccc caatcgctgg ccgccaaact
     6061 gcgctttgcc gccgagcggc tgctgcgtga caagagaata gggtcccgtc gagcgggcgt
     6121 ggatcttatc gtcgaccaag tgatgcagct tcataatata catcacgcca acggtgactt
     6181 tgccgtcgaa agcttcgccg gtgcggccat cgtataacgt cgcctgtccg gtggccggca
     6241 agccggcctt gcgcaacaag ttgaaaattt cttcttccgc agcaccgtcg aagaccggcg
     6301 acgctatgcg cacgccgtcg cgatacttgg acgcgagctt caatacctcg acatctttag
     6361 cgccatcgat taattcgttg gcgtctcgag tgttaaggta gtctttcaat cggcgccgca
     6421 actgatcgat gtctccattg tgagtacgca aaagatcgtc gatttgatgt cccagactac
     6481 gcgccgccca acccaaatgg gcttcgagta tctgtccgac gttcatgcgc gaagggacac
     6541 ctaacggatt caggacgacg tcgacaggtg tgccgtcttc taaatacggc atgtcttctt
     6601 cgggcagaat ccgggagatg acacccttgt tgccatgccg gccggccatc ttatcgccga
     6661 cctggagctt gcgttttata gcaacaaaaa ccttgaccat cttgatgacg ccgggaggta
     6721 gctcatcgcc gcctttaagc ttttcgatct tgtcttcgaa tgcatgcttg atcagattaa
     6781 cctgagtagt cgtgctttcg atgattcgac cgagctcatt ctcaactgat tcgttatcaa
     6841 cttggatctc gctccaataa gacatcggta gctgatcgag atcttcctcg gtcatctcct
     6901 ggcccttatt gagcaaaact ttccggctgt cgtcgctgat acgactcgct aagcgacggc
     6961 caaccagaag ttttttgagc cggcggagcg ccccttcttg gagaatccgc acctcgtcat
     7021 gctggtcctt gcgtaaacgc gtaacctctt cgtcctcgat ggacttgctg cgatcgtctt
     7081 tgcccaagcc cttgcgcgag aaaatacgaa cgttgataac cgtgccttcg acgccgggtg
     7141 gcactcgcag cgaggtgtcg cgcacttctc cggctttttc gccgaatatt gcgcgcaaca
     7201 gtttttcttc cggcgaaagc tgagtctcgc ccttcggcgt aatctttccc accaggatat
     7261 ggcccggctt aacttcagca ccgattcgga taatgccact ttcatcgaga tcggtgagcg
     7321 cctcgtcacc gacattaggg atatccctgg taatttcctc gggccccaac ttggtgtcgc
     7381 gcgaaacgca ctcgaactct tcgatgtgga tcgatgtgta aatgtcttct ttgacgacgc
     7441 gttcattaat caaaattgag tcttcgaagt tgtatccgcc ccagggcatc agagcgacga
     7501 cgacattgcg tcctaacgcc agttcgccca tctcagtcga aggaccatcc gcaataacgt
     7561 cgcccgcctt aacttgatca ccgaccacta tgatgggccg ctgattgtta caagtatttt
     7621 ggttggaacg ctggtattta accaagttgt aaatatcgac accggtatca cgcgagcccg
     7681 ccggcttgtc ggctttgacg acaatgcggg tggaatcgac gctctcgacc aagccgtcgc
     7741 ggcgcgccac aatcgtcacc ccggagtccc gcgccaccgt ccgctccatg ccggttccga
     7801 caaagggcgc ttcggtgcgc aagagcggca ccgcttggcg ttgcatgtta gaacccatta
     7861 gcgctcgatt ggcgtcatcg ttttccaaga acggaataag cgaagccgcg acactcacca
     7921 gctgatttgg cgacacgtcc atgaaattca cttcttcggg acgggccata acgaattcac
     7981 cgcccttgcg cgacgaaacc aagtcggcgg aaaaattgcc gcgaccatcg atcggcgcat
     8041 tcgcctgggc gatcacatgg tcctcttcct cgagtgccga gagatagcga attcggtccg
     8101 taactcggcc gttttctact tcgcgatacg gcgtttcgat aaatccgaaa tcgttgaccc
     8161 gcgcataggt ggttaaggac gcgatcaagc cgatgttcgg accttcaggg gtttcgatcg
     8221 gacagactcg gccgtaatgg gtcggatgca cgtcgcgcac ttcgaaacca gcgcgctcgc
     8281 gcgtcaaacc accgggaccg agagccgata gccgccgctt gtgcgtgatc tcggagagcg
     8341 gattcgtttg atccatgaac tgtgataact ggctggagcc aaaaaactct ttgagcgccg
     8401 ctgacacagg tttgtaattg atcaactcct gcggcatcaa agtctcaatg tcctgcaagc
     8461 tcatcttttc cttgatcgcc cgttccatgc gcaccagccc gacgcggaaa tggttttcca
     8521 cgagttcgcc gaccgcacgc acgcggcgat tgcccaagtg atcgatgtcg tccaccgacc
     8581 cgttgccatt cttgagctcc atcaaatagc gcaccacttc aaggatatcc tctttgcgta
     8641 gcgtaccctg ctccagcgga atgttgagct tgagtcgatg gttgagcttc aaccggccga
     8701 ccttggacag gtcatagcga tccggattga aaaacaaatt gttgaaaaaa ttggtcgccg
     8761 actccactgt tggcggatca cctggccgca agcggcgata gatttcgagg atcgcgtctt
     8821 gcggcgagcg gatcctgtct tgcagtagtg tgtttcgtaa gtagggaccg acactcgatt
     8881 cgtcgataaa cagcacgtca atctgggtga gtcctttgtc gcggattaaa tcgagctttt
     8941 cctgagtgat ttcgtgatta cattcaacta aaacttcttt gccgtgggga tcgacaatgt
     9001 cgtgcgccgc cacgcggccg agaatttcct caagggcgat cggcacctgc tttaaacgcg
     9061 ccgcttccat ttggcgcagc actggccggg tgaacttttt gccttctttg acaataatat
     9121 cgctgctttt aggatcacgc acatcggcgc ttaccttagc gccgagcaga tgattggcat
     9181 tgaactctac cgtcaccttg cggccgtcga aatgaatcgt gtcactgcga tagtagtaat
     9241 taagtaagtc ctcgctggtc atgccgagag cacgtaataa gaccgtcgca tggaatttac
     9301 gccgtcggtc gatgcgcaca tagaccacgt cacgtggatc aaattcgaga tcaacccacg
     9361 acccacggta gggaattacg cgcgccgaat acagtaattt gccgctcgcg tgggtcttgc
     9421 ccttatcatg ctcaaaaaat actccgggtg agcgatgcaa ttggctgaca atgactcgct
     9481 cggtaccgtt gatcataaag gtaccattga tcgtcatgag cggaacttca ccgaaataga
     9541 cttcctgctc tttgacattc ttgatgctgc gcgcgccggt ctgctggtcg acgtcccaca
     9601 agaccaactg aaccgtcacc tttaacgggg cggcgtaagt catgccccgc tggtggcact
     9661 cttctacgtc atatttgggc tcgcccagag tataactaac aaattccaag gaagcggttt
     9721 cgttaaaatc cttaatcgga aagaccgatt taaaaacttc ctgcaatccc atattctggc
     9781 gttcgttggg cgccgcatcc ttctgcagga acagctcgta agagtttttc tggatttcga
     9841 tcaaataggg gagatcgatt atttttcgaa ttctcccgaa gctgcggcgg aatcgtaagt
     9901 tattggcaac c
//
LOCUS       DATLCH010000042         7143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1642337, whole genome shotgun sequence.
ACCESSION   DATLCH010000042 DATLCH010000000
VERSION     DATLCH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642337"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VMZ02_01195"
     CDS             <1..1066
                     /locus_tag="VMZ02_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUR70569.1"
                     /translation="FQSTVELAETVGASVYDINKRLGFPNKHPLSVSFHNDAFNGVDL
                     VTALDVVDWTRGSHKLNTQTREVQVATAADCKWIDVGFADIEISKWATDYNKHHNWDH
                     RVLGDSALSIPALTAACKKRIDGNKGLASRITDRKKLIGERHERQWQKWQDQVKEQWD
                     ERPMTEARLAHEAWQVIKDEDWVLTANTLKEWVHKVWDFDKPYRHLGRELGTGTQIGM
                     SIGAALAHKGSGKLVVDIQPDGDLMFDLGALWMPIKYNIPMLILMYNNRAYYNDWAHQ
                     IHMAHQRGTDPQRAYIGMDLEGPPPDFAHIAKGIGWYAEGVIEDPAEVGPALKRAIAQ
                     VKAGKPALVDAVVWRRGENA"
     gene            1198..2970
                     /locus_tag="VMZ02_01200"
     CDS             1198..2970
                     /locus_tag="VMZ02_01200"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR70570.1"
                     /translation="MAASTIKKSEKRWQSDVIVDMIKKYHFEYIALNPGASYRGLHDS
                     LVNYGENDPPMLTCQHEKIAVQIAHGYARAKGKPMIAIVHNLVGLLHAPMGIYYAYLD
                     RAPVFIMGATGPLAEPKRRPFIDWIHTANVQGEAIRNFVKWDYQPSTVDGVPGAFARA
                     YSIMMSARQGPIYMCYDAGLQEAELDHDVEMPMDGAVEMPTPPAADPLALAKAADTLA
                     GANRVAIIADFAARPPHGWNHVVELAETLGASVWDVGSRLNFPTDHPLNLTMDPDGCY
                     EGVDVVLTLDIADFEKPTHVRDIATRTVVSKVPAGATWIDIGFTDIETSKWSMDYARP
                     YYAQQRMIADVVSAAPVLTKLLKERIAKTAGLPEKIARRSEAIGKRHAETRAKWAKQA
                     QEHWDQKPLTVPRLASEVWEAIKGEDWVLTAGDLGSWGKKLWNFDRPYCHGGRELGTG
                     TQIGLSLGVALANKGTGRLVVDLQPDGDLMFDAGSLWIAAKYQIPMLVVMFNNRAYYN
                     DWNHQLVLARTRGTDPNRAHLGMDLYGPDPDFAGLARSMGWYAEGPIENGDELKGALK
                     RAIEQVKKGKPALVDAICDRRNHG"
     gene            3365..4891
                     /locus_tag="VMZ02_01205"
     CDS             3365..4891
                     /locus_tag="VMZ02_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUR70571.1"
                     /translation="MASEFDIDKPMEEIFDEKEISELAPADAESFSSINETGAEAEPE
                     VKETDFAMSSVQQYLRDIGAVPLLSREREIELAMRIEAGQNEILRALFSAPSALRYVI
                     QLGRKVADGELEINEVLEKPDDHDKEATDGQDPKVFLQAVARLRRLGQSQEDLHRELG
                     HKRISKERYASLTEKEAALTAKICALIQNLHLAAAHVDAWIVRLKRAAHRVEELEQQR
                     TRATRAQKNSISEEIESIEESVGRDVTVIKRLAAELAHGEQTVQIARKEFTEANLRLV
                     VSIAKKYMNRGLAFLDLIQEGNLGLMRAVDKFDYRLGFRFSTYASWWIRQAITRGLID
                     TGHTIRVPVHRVESRNKVMQTAREMQRRLGREPMPEELAKELGISVQEMLNSVQSHGE
                     PVSLHTPVWEDGDELGDFVEDRIGPQPESQALESTLCSEVRKALSVLTPRQEAVLRMR
                     FGIELKRDYTLEELGQKFSVTRERIRQIEQKSLQILRNPNRRKPQTPLAKDGSATQLN
                     "
     gene            complement(5007..6164)
                     /locus_tag="VMZ02_01210"
     CDS             complement(5007..6164)
                     /locus_tag="VMZ02_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70572.1"
                     /translation="MLGTDMKVAETPKEGLKDYIKIDPYSEWLKNEGVQVYQEYYFPS
                     LAKIELGPWERKGGSGAVVHINNNHMPNDCHVVEIKPGGKSEPEHHMYELTIYVVSGR
                     GATSIWQDEKKKSSFEWQAGSLFSIPLNCWYQNFNGSGQEPTRYIAVTNAPPMMRLYR
                     DNNFIFNNPYMFTTRYAGEEDYFSGKGKLFTRRVWESNFITNAPDMLLYGWKERGAGG
                     INAMLEMADNNTKSHISEFPIGTYKKAHRHGPGAHLVLLSGTGGYSLIWTKEDRSDMI
                     KCDWALGSMVTVPSDNCYHQHFNSGTTRARYLALRPGDMGLYKKGRGGGGENADKSLK
                     EGGWQIEYEDEEREIHQIFEKELKAHGATCKMKAFIPWCTGEVGPTSERDT"
     gene            complement(6301..7038)
                     /locus_tag="VMZ02_01215"
     CDS             complement(6301..7038)
                     /locus_tag="VMZ02_01215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR70573.1"
                     /translation="MRAAIKSNVVVWRDDAIVAEYACASALQAPEQIILNHLRSRLSS
                     MRMLDIGVGAGRTMLHFAPRVREYWGVDYSPAMVAACALRRPRSDNVRLAVCDARAMA
                     QFSDNFFDFVLFSYNGIDYVPHRDRLAIFAEIRRVSAVDGLFCFSSHNLQHLPLNGQF
                     EIKSQTVGNLRRLKRLRSWPYALVNDGVHGGRLVTYYVNPTEQLKQLAPYYKVVKVFR
                     LDGASVGDENSLASVDDCWLYYLCAVR"
BASE COUNT         1680 a         2003 c         1926 g         1534 t
ORIGIN      
        1 ctttcaatcc accgtcgagc tcgccgaaac cgtcggcgca tcggtctacg atatcaacaa
       61 acgcctcgga ttccccaaca aacatccgtt gagcgttagc tttcacaacg acgcgttcaa
      121 cggcgtcgac ctggtcaccg cgctggacgt cgtcgactgg acccgcggct cgcataagtt
      181 gaatacccag acccgcgaag ttcaggtggc gacagcggcg gactgcaagt ggatcgatgt
      241 cggtttcgcc gacatcgaga tcagcaagtg ggccaccgac tacaacaagc atcacaactg
      301 ggatcatcgc gttctgggcg acagcgcact gtcgataccg gcgcttacgg cggcgtgcaa
      361 gaaacgcatc gacggcaaca agggactggc aagtcgcatc accgatcgca aaaaattgat
      421 cggcgaacgc cacgaacggc aatggcagaa atggcaggat caagttaagg agcagtggga
      481 cgaacggcca atgactgaag ctcgcctggc ccatgaagct tggcaggtga tcaaagacga
      541 agactgggtg ttgaccgcca ataccttgaa agaatgggtg cacaaagttt gggatttcga
      601 caagccctat cgtcacctcg ggcgcgagct cggcaccggc acgcagatcg gtatgtcgat
      661 tggcgctgcc ctcgcacaca aaggcagcgg taaactggtc gtcgacatcc aacccgacgg
      721 cgatctgatg tttgacttgg gcgcgctgtg gatgccgatc aagtacaaca tcccgatgtt
      781 gatcttgatg tacaacaacc gcgcgtatta caacgactgg gcgcatcaaa ttcacatggc
      841 gcaccagcgt ggcaccgacc cacagcgggc gtatatcggc atggacctcg aaggtccgcc
      901 gcccgatttc gcccatatcg ccaagggtat cggctggtac gccgaaggcg tcatcgaaga
      961 tcccgccgaa gtcggccctg cattgaaacg tgccatcgcc caagtcaaag ccggcaaacc
     1021 cgcgctcgtc gacgccgtag tgtggcgccg cggcgagaat gcctaagaaa agaatagccg
     1081 caaaaaacgc aaaggtcgca aaaaagaatt cgcttaagcg ccaagattct gttttgtaat
     1141 ttttgcgctt ttgcgttctt tgcggccgat cggtttaatt ataaggagga tatctgcatg
     1201 gcagcttcga caatcaagaa atccgagaaa cgttggcagt ccgacgtgat cgtcgacatg
     1261 atcaaaaaat atcatttcga gtacatcgcg cttaatccgg gcgcgagcta tcgcggattg
     1321 catgactcgc tggtcaacta cggggagaac gatccgccga tgctcacctg ccagcacgaa
     1381 aagatcgccg tgcagattgc tcatggctac gcccgtgcca agggcaagcc gatgatcgcc
     1441 atcgtgcata acctggtcgg tctgctacat gcgccgatgg gcatctacta tgcctatctc
     1501 gatcgcgcgc cggtgttcat catgggcgcg accgggccgc ttgccgaacc caagcgccga
     1561 ccgttcatcg actggattca cacggccaac gttcagggtg aagcgattcg taatttcgtt
     1621 aaatgggatt atcaaccgtc caccgtcgac ggagtacccg gggcgtttgc ccgcgcctat
     1681 tcgatcatga tgagcgcgcg ccagggaccc atctatatgt gctatgacgc cggcttgcag
     1741 gaagccgagc ttgaccatga tgttgaaatg ccgatggatg gtgcggtcga aatgcctacg
     1801 ccaccggcag ccgacccgct cgctctcgcc aaagccgccg acactcttgc tggtgcaaat
     1861 cgcgtcgcga tcatcgccga ctttgccgcc cgcccgccgc atggctggaa ccatgtcgtc
     1921 gagctcgccg aaacactcgg cgcatcggta tgggatgtcg gctcgcgttt aaactttcca
     1981 acggatcatc ctttgaatct caccatggac cctgacggct gctatgaagg cgtcgacgtc
     2041 gtgcttaccc tcgacatcgc cgacttcgaa aagcccaccc acgtgcgcga catcgcgacc
     2101 cgcacggtag tcagcaaggt tcccgctggt gctacttgga tcgacatcgg atttaccgac
     2161 atcgaaacca gtaaatggtc gatggattat gcgcgcccct actacgcgca gcaacgcatg
     2221 atcgccgacg tagtttctgc cgcacccgtg ctaactaaac ttttgaaaga acgcatcgcc
     2281 aagactgcag gattgccgga gaaaatcgcc aggcgctcgg aagcgatcgg caaacgccac
     2341 gccgagaccc gcgccaaatg ggctaagcaa gcccaggagc attgggatca aaaaccgctc
     2401 accgttcccc gcctcgcctc cgaggtgtgg gaagccatca aaggagagga ttgggtgctt
     2461 actgctggcg atctcggttc atggggtaaa aagctgtgga acttcgaccg gccgtactgc
     2521 catggcggcc gcgaactggg aacgggaact cagatcggtc tgtcgttggg tgtcgccctg
     2581 gcaaacaagg gcaccggccg gctggttgtc gatctacaac cggacggcga tctgatgttc
     2641 gatgccggtt cattgtggat agcggctaag tatcagatcc cgatgttggt cgtgatgttc
     2701 aataaccgtg cgtattacaa tgactggaac catcagctgg tcttggcacg aacccgcggc
     2761 accgatccca accgtgccca tctcggcatg gacctgtacg gtccggaccc tgactttgcc
     2821 gggttggctc gctccatggg ctggtatgcc gaaggcccaa tcgagaatgg cgacgagcta
     2881 aaaggcgcgc tgaagcgggc cattgagcaa gtaaaaaaag gcaaacccgc ccttgtggat
     2941 gcaatctgcg atcgtagaaa tcatggctaa cgggttatct gccccgcgtc tatcgggaca
     3001 cacgcggggc agatttctca ctactggtaa taaggttttc gaacgcgcat agtgtctgat
     3061 cgctcgtttg gtatcctgcc catttcaagg gcattttagg agtattacga ttatcggcaa
     3121 ccttgacaga tcgctcggtc gtccttaact taacgaagca tgcaacacat taattttatt
     3181 aatcaaatgt tcatactagc taaggtttcc acaccccaca ggagcgcacg agtcagtttg
     3241 acgggctcct gcgccaatct tgcgaactga tcgacgcctt ggcgcgtcta atgctaaagc
     3301 ataactaggg aaaacagata ttggaaccgg aatagccctt tccgggttat tcaaggagag
     3361 tacagtggca agcgaattcg acattgataa gcccatggaa gagattttcg atgaaaagga
     3421 aatttctgag ctggccccag ccgacgcgga gagctttagc tcgatcaacg aaaccggtgc
     3481 ggaagccgag cccgaggtta aggaaaccga cttcgcgatg agttcggtcc agcagtacct
     3541 gcgcgacatt ggcgcggtgc cgctgcttag ccgcgagcgt gaaatcgaac tggccatgcg
     3601 tatcgaagct ggacagaatg agattttgcg tgcgttattc tcggcgccgt cagccttgcg
     3661 ctatgtgatc cagctcggtc gcaaagtcgc cgacggtgaa ttggagatca acgaagtttt
     3721 ggaaaagccc gacgatcacg acaaagaggc caccgatggt caggatccaa aggttttcct
     3781 ccaagcggtc gcccgattgc gccgcttagg ccagagtcag gaagacttgc atcgtgagct
     3841 tggccataaa cgtatatcca aagaacgtta cgcgtcgttg actgaaaaag aggccgctct
     3901 gactgcgaag atctgcgcgt taattcagaa ccttcatctg gccgctgctc atgtcgatgc
     3961 gtggatagtc cggctgaaac gcgccgctca tcgcgtcgag gaactggagc agcaaagaac
     4021 tcgcgcgact cgcgcgcaga aaaactccat cagcgaagag atcgagtcca tcgaggaatc
     4081 ggttggccgt gacgtgactg tcatcaagcg gctcgccgct gaacttgccc atggcgagca
     4141 gacggttcag atcgcgcgca aagagtttac tgaggccaac cttcggctgg tcgtcagcat
     4201 cgcgaaaaaa tatatgaatc gtggattggc atttctggat cttatccagg aaggcaatct
     4261 cggcctgatg cgcgccgtcg acaagttcga ttatcggctg ggctttcgtt tttccaccta
     4321 tgctagttgg tggatccgtc aagcgatcac ccgcggtttg atcgacacgg gtcatacgat
     4381 ccgcgtgccg gtacatcggg tggaatcgcg caacaaagtg atgcaaacag cccgtgagat
     4441 gcagcgtcgt ctcggccgag aaccaatgcc cgaagagttg gcaaaggagc tgggaatctc
     4501 cgtgcaagaa atgctcaact cggtgcagag ccatggagag cccgtgtcgc tccacacccc
     4561 ggtgtgggaa gatggcgacg agctcggcga tttcgtcgag gaccgcatcg gaccgcagcc
     4621 ggagagccag gcgttggaaa gcactctctg ttccgaagtt cgcaaggcac tctccgtcct
     4681 cacgccgcgc caagaagcgg tattgcgcat gcgcttcggc atcgagctca aacgtgatta
     4741 cacgctcgaa gagctcgggc agaagttttc cgtgacacgc gaacgcattc gtcagatcga
     4801 gcagaaatcg ctgcaaattt tgcgtaatcc caatcgtcgc aaaccgcaga ccccattggc
     4861 caaggacggc tcggcaacac agctcaacta actttcgctc cattaacgcg caaaaggttc
     4921 aacgtcgtga aacgttgaac cttttgcttt ttcatgcgaa tagccgcgca actccagcgc
     4981 acagctctcg gaaacgttgg aaaaacttaa gtatcgcgct cgctggtcgg cccgacttcg
     5041 ccggtgcacc acgggatgaa tgctttcatt ttgcacgttg cgccgtgcgc cttcagttct
     5101 ttctcgaaga tctggtgaat ctcacgctct tcatcttcgt attcgatctg ccagcctcct
     5161 tctttaaggc tcttatcggc attctcgccg ccgccgccgc ggcctttttt gtacagaccc
     5221 atatcgccgg ggcggagcgc caaatagcgg gctcgagtcg tgcctgagtt gaaatgctgg
     5281 tggtaacaat tgtcgctcgg gacagttacc atggagccga gcgcccaatc gcacttgatc
     5341 atgtccgagc gatcttcttt ggtccaaatc aatgaataac cgccggtgcc gctcagcaac
     5401 actaaatggg cgcccggtcc atgacggtgt gcttttttat aagtaccgat gggaaattcc
     5461 gatatgtggc ttttggtgtt gttgtcggcc atttccaaca tggcgttaat accgccggcg
     5521 ccgcgctctt tccagccata aagcaacatg tccggcgcat tggtgataaa atttgactcc
     5581 cagacgcggc gggtgaacag cttgcctttg ccgctgaagt agtcttcttc gccggcatag
     5641 cgcgtcgtga acatatacgg attgttgaaa ataaaattgt tgtcgcgata cagccgcatc
     5701 atcggcggcg cgttggtgac tgcgatgtag cgagtgggct cctggccgct accgttgaag
     5761 ttctggtacc agcaattgag cggaatcgaa aacaagctgc ccgcctgcca ctcgaaactg
     5821 cttttctttt tctcgtcttg ccatatcgac gtcgcgcccc gtcccgagac cacataaatc
     5881 gtcaactcgt acatgtggtg ttcaggttcc gatttcccac ccggcttgat ttcgacgaca
     5941 tgacagtcgt tgggcatgtg attattgttg atgtgaacca ccgcgccact gccacccttg
     6001 cgctcccatg gtcctaattc gattttcgcc aagctcggga aataatattc ctggtagact
     6061 tgcacgcctt cgtttttcag ccactccgaa taaggatcga ttttaatgta atctttgaga
     6121 ccctctttgg gagtctccgc aactttcata tcggtgccca acataaccac aggtagttcc
     6181 ctcctctgcc gacgttagta acgcggctga accgtgagtc atcgcgactt cttaatgaaa
     6241 tatccgaagc aggccgacct tgtcaacgag tggccggaag gaaccgtgtg gaacgatcat
     6301 tcatcgtaca gcgcataagt aatacaacca gcagtcgtcc actgacgcta atgagttttc
     6361 atcgccgacg gatgcgccat caagccggaa cactttcacg actttgtagt acggcgctag
     6421 ttgtttcagc tgctcggttg ggttgacgta gtatgttacc aagcgacccc catgaactcc
     6481 gtcattgacc aatgcatacg gccaactgcg cagccgtttg agacggcgca gattaccaac
     6541 tgtttgggat ttaatttcga attgcccgtt gagtggcaga tgctggagat tatgggacga
     6601 aaaacaaaac aggccgtcaa cagcgctaac ccgtcttatt tctgcaaaaa tagcgagccg
     6661 atcccgatgc ggcacataat cgatgccatt atagctgaac agcacaaaat caaaaaaatt
     6721 gtcggaaaac tgcgccatcg cgcgcgcatc gcacaccgcc aagcgaacgt tgtccgaacg
     6781 cggccgacgc agagcacacg ccgcaaccat ggccggcgag tagtcaacac cccaatattc
     6841 acgaaccctc ggcgcgaaat gcagcatggt tcgaccggcg ccaacgccta tatccagcat
     6901 cctcatgctg gataaacgag atctaaggtg gttgaggatt atctgttccg gtgcttgcag
     6961 cgcgctggcg caggcgtatt cggcgacaat cgcatcatct cgccatacga cgacatttga
     7021 cttaattgcc gctctcatcg gcagcggttt cggcgcgcaa aaccgctagt ctgtctcacc
     7081 cgcagcaatg tcgtaaagtg cggatgtatc atttccggca caatcagcct agatattaat
     7141 att
//
LOCUS       DATLCH010000043         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_154332, whole genome shotgun sequence.
ACCESSION   DATLCH010000043 DATLCH010000000
VERSION     DATLCH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154332"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..2107)
                     /locus_tag="VMZ02_01220"
     CDS             complement(269..2107)
                     /locus_tag="VMZ02_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HUR70574.1"
                     /translation="MQPTRDTRQLNWFLAWAVVFCDVGTSVYYVPGIMYGHVKDATPF
                     FVLLTTFGFILLSLKYIEISWRNPEGGGVVTISTKAFGPIWGCLGGMLITVDYFLTTA
                     ISAVSGFQYLGSAFPVLDPYIVLLSCAGVAMLAALNIVGIRESAAVSLVMACSSFLVN
                     LVVIGAAFVHMDNAQWHTVISRLTISKGISSYELLVGFASAWLAFSGLESISQLSPTM
                     RLPLRRTTRWAMGAVIVTMVITSPMLTAMSIGLLPEHFKATESERFISELGLIVGGLG
                     IKFAVVLTATSLLLFAANTAIIGAYHVFLALVKGNFMPKILAARNTTFNSPHIAVAIA
                     TAVPIVIILMSRGEMKVLGDMYAFGLLGAFVFSSLSLDRIRWNLGRRDVGFWIGVLTT
                     LMVMLAWGVNLVEKQLATLFGGGVTLVGLIIAIGVRRAWFVDLLVQIPFLERLQSRAY
                     RASEDLVEDELQGLMTLSEAVEVKPLYASSTLLALRDQSLGLVQEAVIRVKGKGENAL
                     YCIYVEEWPGLFAGETPHVPNEQGVQTLKAVLQAVRERGLEIIPIWSISHNAAEAIAN
                     AAKALDVDSVIIGQSRRSAFYHMLRGHVIKGLMKKLPHDCHLMICN"
BASE COUNT          569 a          595 c          611 g          415 t
ORIGIN      
        1 tccgaaagcg tgtgaacgtg gccaacgccc gtgaagtccg catacaagtt actggcgtac
       61 agcgttatca gcagaattac tgaggagcag aggaatcgct tcatggaatc atgtcctgca
      121 gcctactgga gtcaaatctg cggtgtcaat gagtcggtgc cgtgtaaacc cggatgtcgt
      181 ctctgccatt ggggatcgag ctaaacaaat tttaatcggg ccaaaatcat tcgccctgcg
      241 cgcaggaaat cagccaaatt gacgtgactc aattgcagat catcaagtgg cagtcgtgcg
      301 gcagcttctt cattaatcct ttgatcacat ggccgcgcag catatggtag aaagccgagc
      361 ggcgcgactg gccgataatc accgaatcga cgtccaaggc tttagcggcg ttggcgatcg
      421 cttcagccgc attgtgggag atcgaccaaa tcggaatgat ctcgagacct ctttcgcgca
      481 cggcttgtaa cactgctttg agagtttgaa ctccttgctc gttgggcacg tgaggagttt
      541 ctccggcaaa caagccgggc cactcttcaa catagatgca ataaagcgcg ttctcgcctt
      601 tgcctttgac gcgaatcacg gcttcttgca cgaggcctaa actctgatca cgcagggcaa
      661 gcaaagtcga agaggcatac aaaggtttga cttccacggc ttcggagagc gtcatcagac
      721 cttgcaactc gtcttcgaca aggtcctcgg acgcgcgata agcgcgcgac tgcaggcgtt
      781 ccaaaaaagg tatctgcacc agcaggtcga cgaaccaggc gcgacgcacg ccaatcgcaa
      841 taataagtcc gaccagtgta acaccaccgc cgaagagggt tgcgagctgc ttttcgacga
      901 ggttgacgcc ccacgcgagc atgaccatca acgtcgtcag cacgccgatc caaaacccga
      961 catcgcgccg gccaagattc caacggattc tgtcgaggct cagggatgaa aacacaaagg
     1021 caccgagcag gccgaaggcg tacatgtcgc ccaagacctt catctcaccg cggctcatga
     1081 gaataatcac tatcgggacg gcggtcgcga tggcgacggc gatgtgcggc gaattgaaag
     1141 tggtgttgcg cgctgcgagt attttcggca tgaaattgcc cttcaccagc gccaggaaaa
     1201 cgtggtacgc gccgataatg gccgtattcg cggcaaacaa gagtaagctc gtcgcggtca
     1261 ggacgacggc aaacttgatg cccaaacctc cgacgattaa gcctaattcc gatatgaagc
     1321 gctcgctttc ggtggccttg aagtgttccg gcaagagtcc gatggacatt gcggttaaca
     1381 tcggcgaggt gatcaccatc gttacgatta cagcgcccat cgcccagcgg gttgtgcggc
     1441 gcagcggcaa acgcatggtg gggctcaact ggctgatgct ttccaggccg gaaaacgcca
     1501 gccacgcgga agcgaatccg actaacaatt catacgatga aattcctttg gagatggtaa
     1561 gtcggcttat gacggtgtgc cattgggcgt tgtccatgtg cacaaaggcc gcgccaataa
     1621 ccaccaaatt gaccagaaaa gaggaacacg ccatgacgag agaaacggcg gcgctctcgc
     1681 gaataccgac aatgttcaac gccgccagca tggcgacgcc cgcacaagac aaaagcacga
     1741 tatacggatc aagaaccgga aaggcgctgc cgagatactg aaatccggag accgcagaga
     1801 tcgcggttgt cagaaagtaa tcgaccgtta tcagcatccc gccgagacag ccccaaatcg
     1861 gaccgaaggc tttggtgctg atcgtgacca cgccaccacc ctcggggttg cgccaggaga
     1921 tctcgatgta tttgagcgac agcagaataa aaccgaaggt ggttagcaaa acaaaaaacg
     1981 gcgtggcgtc tttgacgtgg ccgtacatga tgccgggaac gtaatagacc gaagtaccga
     2041 catcacagaa gaccaccgcc caggcgagaa accagttgag ctgtcgcgta tcacgggttg
     2101 gctgcatgat gaatcgccga ggagacggcc actataggaa ccaaaagcac aggatgcaat
     2161 cggctcgagt tgtgtagtgc gcttcgctgc
//
LOCUS       DATLCH010000044         2862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4619734, whole genome shotgun sequence.
ACCESSION   DATLCH010000044 DATLCH010000000
VERSION     DATLCH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4619734"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1102)
                     /locus_tag="VMZ02_01225"
     CDS             complement(152..1102)
                     /locus_tag="VMZ02_01225"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70575.1"
                     /translation="MTKLKMSLALGSYDRHAPLLEGCIQHPDLDIQYIECDPQQGRHE
                     RFLQHFEFDAAELSFSSYCIAIDQGMPVHAVPIFPRRLFSQSQMYKNVNCGIQSPEDL
                     VGKRIGLSGYQNTLGVRAKGDLTHFYGVDRSSVTWITPGKEVIDVELPKDLKIEIRDS
                     MAEMEQEFVDGKIQAMFVSRIPKPFRDGDKNLARLFGDSQAEEERYLREEGYFPIMHV
                     LAYKKELAQQYSQLPRALFEIFEQARSRATQRWVDPNWSLAIWARRELERQNQLCTFD
                     PWKNGLEANRKNIERFALYSHEQGLTKRRLTPEELFVAID"
     gene            complement(1280..1864)
                     /locus_tag="VMZ02_01230"
     CDS             complement(1280..1864)
                     /locus_tag="VMZ02_01230"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70576.1"
                     /translation="MIRRLSVLLLFGLLSSLPRSVHAGEATAQLSATINEFVTILVST
                     PVSELRTTGLPEKALKLIYSRFDFIEMTKRSLGPHWKTLEPAEQGEFVDGLTHRLLVT
                     YGRTVRASGDEKIVFKKETLDGGYAKVESAVVGGNSDTLIDYRLHDVDGQWRVYDMVI
                     EHVSIVSNFRAQFEREISRTSVKALLEKIKQRQS"
     gene            2044..2484
                     /locus_tag="VMZ02_01235"
     CDS             2044..2484
                     /locus_tag="VMZ02_01235"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HUR70577.1"
                     /translation="MYYFAYGSNLSWLQMQRRCPSSRFITIAKLADYDFAIARHSRLR
                     DCGTANVYPAAGKEVWGAIYDVSEADLLVMDGFEDGYHREILSAYTSGERSETLQVLV
                     YVAPIESGVPLPNPEYKRLILEGAHHWKFPSSYIAMLETIQTRG"
     gene            2576..>2862
                     /locus_tag="VMZ02_01240"
     CDS             2576..>2862
                     /locus_tag="VMZ02_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70578.1"
                     /translation="MAPVPADLRTKTSNIVRHPRTRKLAIWFAAIVVAIGVLLGLVAP
                     PLLRGKIASELSKKLHRPVAIEQIKINPYTLTLTVRGFLMQEKQSQTPALS"
BASE COUNT          646 a          782 c          744 g          690 t
ORIGIN      
        1 agttacgacc tgtcgtttac gattttttcc tgcgcgctgt tcacgtccgc gtttttggta
       61 ctgctagcga agccgccgcc ccacgagtcg acaacttccc gcagcaaggc gacggtagaa
      121 tctaaaaacg aaagtcaaaa ctagaactgg tttaatcgat cgccacaaac aactcctccg
      181 gcgtcaatcg ccgcttggtc agcccttgtt cgtggctata gagcgcgaag cgctcgatat
      241 tcttccggtt ggcttcaagg ccgtttttcc aaggatcgaa ggtgcacagc tgattctgcc
      301 gctctagttc gcgccgtgcc catattgcca gcgaccagtt ggggtcgacc caacgttgtg
      361 tagcacggct gcgggcttgc tcgaagattt caaaaagcgc gcgcggcaac tgcgaatact
      421 gctgcgccag ctctttctta taggcgagca catgcataat cgggaagtag ccttcttcgc
      481 gcaagtagcg ctcctcttcg gcttgtgaat cgccaaatag acgggccaag tttttatcgc
      541 cgtcacggaa aggtttgggt atccgcgaca cgaacattgc ctgtattttg ccatcaacaa
      601 actcctgttc catctcagcc atgctgtcgc ggatttcgat ctttaggtct ttggggagtt
      661 cgacatcgat cacctctttg ccaggagtaa tccaggtcac tgaactgcga tcgacgccgt
      721 agaaatgcgt caggtcgcct ttggcgcgca cgcccaaagt attttgataa ccgctcaggc
      781 caatgcgttt accgactaaa tcttcgggcg attgaatgcc gcaattgacg ttcttgtaca
      841 tttgcgactg gctgaacagc cggcgcggaa agatcggcac agcatgcacc ggcatcccct
      901 gatcaatggc gatacagtat gacgagaaag ataactccgc ggcatcgaac tcgaaatgct
      961 gcaggaaccg ctcgtgtctc ccctgctgcg gatcgcattc gatatattgg atgtcgaggt
     1021 cggggtgttg aatgcagcct tcgagcaacg gcgcgtgccg atcgtaggaa cctagggcca
     1081 atgacatttt tagcttggtc atgtcggacc aagtatccga ctgcgctcag ctcgtcaacg
     1141 gctggcgttc ttcgagaaaa atagatttct tggtgtacga tatgcaactc acgcgtttga
     1201 aaaacaaatc ggcctaagct catcactgaa cttaggccga tgaaaagttt tttaggggag
     1261 atattagctg cgctggattc taggactggc gctgcttgat cttttcaagc aaagccttca
     1321 ccgaagtgcg cgagatctcg cgctcgaact gcgcgcggaa attgctgact atgctcacgt
     1381 gttcgatgac catgtcatag acgcgccact gaccgtcaac atcatgcaga cggtaatcga
     1441 ttaaggtatc ggaattgccg ccgacgactg cggattcgac cttggcatag ccgccgtcca
     1501 aggtttcttt tttgaagacg attttttcgt ctcccgacgc gcgcacggtg cgcccgtagg
     1561 ttacgagcaa ccggtgagtc aaaccatcga cgaactcgcc ctgctctgcc ggttccagcg
     1621 tcttccaatg aggtcccaag gaacgcttag tcatctcgat aaaatcaaag cgactgtaaa
     1681 tcagcttcaa agctttttcg ggcaaacccg tcgtgcgaag ttcagatacc ggtgtgctca
     1741 cgaggatcgt gacaaattca ttgatggttg cacttagttg cgcggtagct tccccggcat
     1801 gaacgctccg gggcagcgag cttaacaacc caaacaacag taaaacgctt aatcttctta
     1861 tcatatggcc ataactcctg tcggcaaaag ttcttaagct aactaggaaa ttacttagaa
     1921 gcaagaagcg tacaatggat tctccaagac ttgccgcaaa ccgggttttt cgcacgttcc
     1981 ctcgcgactt gtgccaggac aaaaagtctt taaaatcgct gctaattctg gagggtcgtc
     2041 gtcgtgtact attttgccta cggatcgaat ttgagttggc tgcaaatgca gcggcgttgc
     2101 ccgtcgtcgc ggtttatcac tattgcgaaa ctggccgact atgatttcgc gatcgctcgc
     2161 cactcacgcc tgcgcgattg cggcacagcg aacgtttacc cggccgccgg taaggaagtc
     2221 tggggcgcca tctatgacgt cagcgaggcg gatctgcttg ttatggacgg cttcgaagat
     2281 ggctaccatc gcgagatatt gtcggcctac acgtctggcg agcgcagcga gaccttgcaa
     2341 gtattggttt acgtcgcgcc catagaaagc ggagtacctc tgcccaatcc ggaatacaaa
     2401 cgcttgatcc tcgaaggcgc gcaccattgg aagtttccca gttcttacat cgcgatgctg
     2461 gagacgatcc agacccgcgg ttaaatttac ttcgctgcgg cagttcgtcg gtttcgatag
     2521 acatgtggta ccccctgcga taggatcacg gcaaattctt ttgcgagggt ttgccatggc
     2581 gccagtacct gccgatctgc gcacaaaaac ctccaacatc gttcgtcacc cgcggacgcg
     2641 taaacttgct atttggtttg cggcgatcgt cgtcgcgatc ggcgtgctgc tcggcctagt
     2701 cgcgccgccg ctcttgcgcg gcaaaatcgc cagcgagcta tcgaagaagc tgcaccgccc
     2761 ggtcgccatc gagcagatca agatcaatcc ctatacgttg acgctcactg tgcgcggctt
     2821 tttgatgcag gagaagcaga gtcaaacacc ggcgctgtcc tt
//
LOCUS       DATLCH010000045         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_154792, whole genome shotgun sequence.
ACCESSION   DATLCH010000045 DATLCH010000000
VERSION     DATLCH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154792"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VMZ02_01245"
     CDS             <1..495
                     /locus_tag="VMZ02_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HUR70579.1"
                     /translation="IFNLLDQIDLKILYILQNNGRKRLADIAVEVELSAPAVMERVKK
                     LEASGVIRGYQALIDSRKVGKDITAFIGVSIGNQSGIDAFARQMLRYGDVLECHHVTG
                     DESFILKVKSANTGSLEKLLGEIRSVDGVTRTVTNVVLSTAKESQALDLDAGLAESAN
                     AKKK"
     gene            552..>2354
                     /locus_tag="VMZ02_01250"
     CDS             552..>2354
                     /locus_tag="VMZ02_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HUR70580.1"
                     /translation="MSLSGLPEKQGLYDPRFEHDACGVGFVVHIKGEKSHEIVAQALT
                     VLENLDHRGACGCEENTGDGAGILMQVPHAFLKEACAGLGFSLPDQGDYGVGMIFLPD
                     HREQRRRFEKIIEAIVKEEGQRCLGWRKVPTDNLYLGETAKATEPFVRQVFIGRSAGL
                     TDDLAFERKLFVIRRRAENAIRYAGLPGGDLFYVPSLSSRTLIYKGMLTPHQVSTFYP
                     DLTDPQLESAIALVHSRFSTNTFPSWGRAHPYRYLIHNGEINTLRGNENWMHARQGML
                     ASEMFGDDLRKVFPIIQEDGSDSTKFDNCLEFLALSGRSLPHAVMMMIPEPWENHESM
                     DAKKRAFYDFHSCLMEPWDGPASIAFTDGTVVGAVLDRNGLRPSRYYVTKDDLVIMAS
                     EVGVLDVPAERVLEKRRLQPGRMFLVDTSEGRIITDEEIKQEMASAQPYGDWLKNNLV
                     RFNDLPEVTDKEPPVDHAKTLQRLQAFGYNFEDLRVNIGPMAQNSIQPVGSMGTDTPL
                     AVLSDKPQLLYNYFKELFAQVTNPPIDPIREELITSTTLTIGSEGNLVNPGPQSCRQL
                     RLPEPILKNADLEKLRQIDMPGIKSVTLPILFNAG"
BASE COUNT          541 a          626 c          685 g          502 t
ORIGIN      
        1 atttttaact tgcttgatca aatagattta aaaatacttt atattctgca aaataacggg
       61 cgtaagcgct tagccgacat tgccgtcgaa gtggagcttt cggcgccggc cgtgatggag
      121 cgggtaaaga agctcgaagc cagcggtgtc atccggggct atcaagcact gatcgacagc
      181 aggaaggtcg gcaaagacat taccgctttt atcggcgtgt cgattggtaa tcagagcggt
      241 atcgatgctt tcgcccggca gatgctccgc tacggcgacg tattggagtg tcaccacgtt
      301 accggcgatg agagtttcat tctcaaagtg aagtcggcca acacgggttc cttggagaag
      361 ctactgggtg agattcgctc ggttgacggg gtgacacgga cggtcaccaa cgtggtgtta
      421 tcgacggcca aagagagcca ggcgctcgac cttgacgctg gcttggccga aagcgctaac
      481 gccaaaaaga aataattgta cgcgtcacca cgcacgaaat gaagccacaa agaagtcgga
      541 aggataaacc catgagtctc tcaggattgc cggaaaaaca aggtctctac gatccgcgct
      601 tcgagcatga cgcctgcggt gtcggcttcg tcgtccatat caagggcgaa aagtcacacg
      661 agatcgtcgc gcaagcgctc accgtgttgg aaaacctcga tcaccgcggc gcctgcggct
      721 gcgaggagaa caccggcgat ggcgccggca tcctgatgca agtgccgcac gcatttctca
      781 aagaagcctg tgccggattg ggtttttcat tgccggacca gggggattat ggcgttggca
      841 tgatattcct tcccgatcat cgcgagcagc ggcggcggtt cgaaaagatt atcgaagcga
      901 tcgttaaaga agagggccag cgatgtctcg gttggcgcaa agtgccgacc gacaaccttt
      961 atttaggcga gactgccaag gcgaccgaac cctttgtgcg tcaagttttc atcggccgca
     1021 gtgctggtct gacagacgac ttggcgttcg agcgcaagtt attcgtgatc cggcgccgcg
     1081 ccgagaatgc catccgttat gccggcttgc ctggcggcga cttattctat gtgccgagct
     1141 tgtcgagccg gacgttgatt tacaaaggca tgctgacgcc gcaccaggtg tcgacgtttt
     1201 accctgatct tacggatccg caactcgagt cggcgattgc cttggtgcac tcgcgcttca
     1261 gcaccaacac ctttccgagc tggggccggg cgcatcctta tcgatatttg attcacaacg
     1321 gtgagatcaa tacgttgcgc ggcaacgaaa attggatgca tgcgcgccag ggtatgttgg
     1381 cctcggaaat gttcggcgac gatttaagga aagtatttcc gatcattcag gaagacggca
     1441 gtgactccac caagttcgac aattgcctgg agtttctcgc tttgagcggc cgttccttgc
     1501 cgcatgccgt gatgatgatg atccccgagc cgtgggaaaa ccacgaaagc atggatgcga
     1561 aaaagcgcgc cttctacgat tttcatagtt gtctgatgga accctgggat ggcccggcat
     1621 cgattgcttt caccgatggc acggtggtcg gcgcggtgct cgaccgcaat ggactccggc
     1681 cgtcgcgcta ctacgtgact aaagacgatc tcgttatcat ggcttcggaa gtcggtgtgc
     1741 tcgacgtgcc ggcggagcgg gtgttggaga agcgacgctt gcagcccggc cgaatgtttc
     1801 ttgtcgacac cagcgaggga cgcattatca ccgacgaaga gatcaagcag gagatggctt
     1861 cggcgcagcc ctacggcgat tggcttaaaa acaatttggt tcgattcaac gacctgcccg
     1921 aagtgacgga caaagagccg ccggtggatc acgcgaagac gctgcaacgg ctacaggcgt
     1981 tcggatacaa cttcgaggat ctgcgcgtca atatcggccc catggcgcaa aacagcatcc
     2041 agccggtggg ctcgatggga acggatacgc cgctggcggt tttatccgac aagccgcagc
     2101 ttctctataa ttatttcaaa gaactgttcg cccaggtgac caatccgccg atcgatccga
     2161 ttcgtgaaga attgattacc tcgacgacgt tgacgatcgg cagcgagggc aacctggtca
     2221 atcccgggcc gcagagctgt cgccagttgc gcttgcccga accgattctg aagaatgccg
     2281 acctggaaaa actccggcag atcgacatgc ccggcatcaa atccgtaacg ctgccgatct
     2341 tgttcaatgc cggc
//
LOCUS       DATLCH010000046         4239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9090833, whole genome shotgun sequence.
ACCESSION   DATLCH010000046 DATLCH010000000
VERSION     DATLCH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9090833"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..388)
                     /locus_tag="VMZ02_01255"
     tRNA            complement(316..388)
                     /locus_tag="VMZ02_01255"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(353..355),aa:Phe,seq:gaa)
     gene            complement(441..1403)
                     /gene="gshB"
                     /locus_tag="VMZ02_01260"
     CDS             complement(441..1403)
                     /gene="gshB"
                     /locus_tag="VMZ02_01260"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HUR70581.1"
                     /translation="MNKLKIAVVMDPVEKISIDKDTTFVLMLEAQRRGHELYFLEIDD
                     LFVRGGAAHARYRSLRLARATPHFELGESTVGVLDDFDSVWMRKDPPFDMKFFFATHL
                     LSLINSSKCFVMNSPRGLREANEKLYAMRFPEQIPQTLVSSNMERLRAFMDELGGELI
                     VKPLDGCGGTGVFYLNGQDRNTNSILEAATDNGRRLVMGQRYLPEIRQGDKRIIVLNG
                     EPLGAVLRVPLESETRGNIHVGGQCVKTEVTERDREICADLAPLLRADGLYFVGLDVI
                     GSFLTEVNVTSPTGIQEINSLNGVRLESQVIDFVESQVEKLAEN"
     gene            complement(1424..1909)
                     /locus_tag="VMZ02_01265"
     CDS             complement(1424..1909)
                     /locus_tag="VMZ02_01265"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HUR70582.1"
                     /translation="MTQAGARPLPTLEPVVEVRWGGFFRRASALIIDLCVVSLLSLVM
                     GAMAYVGYKVGLGAHGLVIAADNATALFAILTIGWLGLTSAYFIVMHGMGGQTVGKRL
                     LGLRVVDSNRQQPSVRQATLRWFAAIGFAPIVIGFLWVLWQREKRGWHDFVARTWVVQ
                     E"
     gene            complement(2256..2996)
                     /locus_tag="VMZ02_01270"
     CDS             complement(2256..2996)
                     /locus_tag="VMZ02_01270"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HUR70583.1"
                     /translation="MARFFIRTESIQETSATLTGPELDHLRRVLRLIVGDIVTLFDNS
                     GREHEAVIRSLNSDQAGLEIRRSYESVKESPLKLNLALGLTKGEKMDFVVEKATELGV
                     ATILPFTSSFTVPKLDENKLAKRVERWRKIALSAAKQCGRTHVPEILPLCEFHRLVEG
                     AGASALKLLFWEKEMAQTLRQVHGTNTEPPAILIAIGPEGGLSTPEAELAMARGFYTV
                     HLGRRILRAETAAVTAVSLVQFLWGDLS"
     gene            complement(3003..3866)
                     /gene="prmA"
                     /locus_tag="VMZ02_01275"
     CDS             complement(3003..3866)
                     /gene="prmA"
                     /locus_tag="VMZ02_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HUR70584.1"
                     /translation="MTESWLHLTVYTSAACLDAVANFLIERGSSGVVFKHRAVEAYFP
                     NNRYADALRRAIHGFVFDVVKLQGRSSKPRLSWQIARSGDWENAWKRFIRPSRVGQRL
                     WVTPPWLTPPKFRSRHVITIEPGMAFGTGTHATTRGCLEFIELVVELSGSEFKALDVG
                     TGSGILAIALVKLGAKKVWAIDNDPIALAVARENVRINHADAKVHLSSAGLNRLKNKF
                     PLVVGNLTAETIIELAGAIEHRVVSGGYVILSGILNDKAAAVLRCFTPRFVLIKRKRA
                     REWVTLLLRRR"
BASE COUNT         1008 a         1228 c         1056 g          947 t
ORIGIN      
        1 ctcgtgcgct tgacaaacgc tgcccgtggc gggttctcca gatatcagcc tgtgagattt
       61 tcagtcggga caacagcacg caccctagaa ctgaaccgag ccgtatgatc gcactaaaaa
      121 acaaaatttg tgagataact gaatcgctgc aatctaacag ctgctgatcc tccaccgcgc
      181 atctacccag agagtatccg tcgaaatctt ccgaaacgtt ctgaactttt tatttctggc
      241 aaagtagaca gtgaaaacgc gacgttcaca ccactcgcaa aaacataact gattggattt
      301 acagaagtta aaatgtggcc agggacggaa tcgaaccgcc gacacggaga ttttcagtct
      361 cctgctctac cgactgagct acctggccag atgaagtgaa actggaatat tgcttaaaag
      421 tttcggcgag tcaagtttag ctaattctcg gcgagctttt ccacttggct ttcgacgaag
      481 tcaatcactt ggctttccag ccgcactccg ttaagtgaat tgatctcttg aatgccggtc
      541 ggactggtta cattgacctc ggtgagaaag ctgccgatga cgtccaatcc aacaaaataa
      601 agtccgtcag cgcgcaaaag tggcgccaag tccgcgcata tttcgcgatc gcgctcggtc
      661 acttccgttt tcacacattg tccgccgaca tgaatgttgc cgcgggtttc ggactctaac
      721 ggcacgcgca agaccgcgcc gagtggctcg ccattgagaa cgatgattcg tttgtcgcct
      781 tggcggattt ctggcaggta gcgttgcccc atcactagcc ggcggccatt gtcagtcgcg
      841 gcttcgagga tcgagttggt attgcgatcc tggccgttca gataaaacac accggtaccg
      901 ccgcaaccgt cgagcggttt aacgatcaac tcgccgccca gttcatccat aaacgcgcgc
      961 aagcgctcca tgttgctgct gaccagcgtc tgcggaatct gttcgggaaa acgcatcgca
     1021 taaagttttt cgttggcttc gcgcagccct ctcggactgt tcatgacaaa gcatttgctt
     1081 gaattgatca gactgagcag gtgcgtcgca aagaaaaact tcatgtcgaa tggtggatct
     1141 ttgcgcatcc acacggaatc gaaatcgtcg agcactccga ccgtagactc gcctaattca
     1201 aagtgaggcg tagcgcgtgc cagtcgcagt gagcgatagc gcgcatgagc ggcgccgccg
     1261 cggacgaaca aatcatcgat ttccaggaaa taaagttcat ggccgcgccg ctgcgcttcg
     1321 agcatcagga caaacgtcgt gtccttatcg atacttattt tctcgaccgg gtccatgaca
     1381 accgcgattt taagtttgtt catactatgt cactctgcgt cacctattct tgaacgaccc
     1441 aggtacgcgc gacaaaatca tgccagccgc gcttttcgcg ctgccaaagc acccagagaa
     1501 acccgatgac gatgggcgcg aagccgatcg cggcgaacca tcgcagcgtc gcttgacgca
     1561 cgcttggttg ttgacgatta ctatcaacta cgcgcagccc gagtagtctc ttaccgacgg
     1621 tttggccgcc cattccatgc atgacgatga aataagcgct ggtcaatcca agccagccga
     1681 ttgtcaggat ggcgaagagc gcagtagcat tgtcggcggc gatgacgagt ccatgcgcgc
     1741 ccaagccaac tttataaccg acgtaggcca tagcacccat gactaacgac agcaaagaga
     1801 caacgcagag atcgataatt aatgcgcttg cgcggcgaaa aaaaccgccc cagcgaactt
     1861 ccaccaccgg ttccagcgtc ggcagcgggc gagccccggc ctgtgtcatt tccgctttgc
     1921 ttggaaactg ccagtcatct gactccgcgt cgctgtcggg ctcattttcc tccgtcgtta
     1981 aaagaaagtt ctcgggcttc ggtactccga agaacactcg ccggtgcagt aacttggcgc
     2041 cgcatctagc gcagagttcg tcgcttggga cgacgggagt ttcgcagttc tgacatttca
     2101 agtttgattc agcgaccatt atcttctaat ccgccgagca atttggaaac accgacataa
     2161 aagtcttaac aatctagcga tccctttatt aggtcacgcc aaaaaccgcg atgccaccct
     2221 atgctaaaag ttgtctgtgc ccaaggcaag ccgcgtcaac tcaaatcgcc ccaaagaaat
     2281 tgcaccaacg acacggcggt gaccgcggcg gtttcagcac gcaaaatgcg ccggcccaaa
     2341 tgcacggtgt aaaagccgcg cgccatcgcc aattccgctt caggagtgct cagaccacct
     2401 tcgggaccga tggcaatgag aattgctggc ggttccgtgt tggttccgtg cacctgccgc
     2461 aacgtttgcg ccatctcctt ttcccaaaat aatagcttga gcgcgcttgc gccggcgcct
     2521 tcgaccaatc gatgaaactc gcacaatggc aaaatttccg gcacatgggt ccggccgcat
     2581 tgctttgccg cgctgagcgc aattttgcgc cagcgttcga cgcgcttggc gagtttattc
     2641 tcgtcgagct ttggcacggt aaagctcgat gtaaacggca atatcgttgc gacgccgagt
     2701 tcggtcgcct tctcgactac gaagtccatt ttctcaccct tggtgaggcc gagggccaag
     2761 ttcagtttca gcggcgactc tttaacagat tcataggacc gccgaatctc gagtccagcc
     2821 tgatcgctgt tcaggctgcg aatcaccgcc tcatgttccc ggccggagtt atcgaacagc
     2881 gtcacgatat cgccgacgat caagcgcagg acccggcgca ggtggtctaa ctctggaccg
     2941 gtcagagtcg cgctggtctc ttgaatactc tccgtgcgga tgaagaaacg agccataaac
     3001 tcctaacgac gccgcagcag taaggtcacc cattcccgcg cgcgtttgcg tttaatcaga
     3061 acgaagcgcg gcgtaaaaca gcgcagtacc gcagcagcct tatcattcag gatacccgac
     3121 aagatcacgt aaccgccgct cacaactctg tgctcgatgg caccggcgag ttctataatc
     3181 gtttctgccg tcagattacc gacaacgagt ggaaatttgt ttttgaggcg atttagcccc
     3241 gcgctggata aatggacttt ggcatcagca tggttgatgc gcacattttc ccgagccacg
     3301 gcaagggcaa tggggtcgtt gtcgatggcc caaaccttct ttgctcccaa cttgaccaat
     3361 gcgatcgcca ggatacccga cccggtacca acatcgagag ctttaaattc actccccgac
     3421 aactccacaa ccagctcgat aaactccaaa cagccgcgtg tcgtcgcgtg agtgccggta
     3481 ccgaacgcca tgcccggttc gatcgtaatt acgtggcgtg agcggaattt tggcggagtt
     3541 aaccagggcg gagttaccca cagccgttgg ccgacccggc tgggccggat aaatcgcttc
     3601 caagcattct cccagtctcc cgaccgggcg atctgccaag acaaacgtgg cttgctgctg
     3661 cgaccttgaa gtttaacgac atcgaagaca aagccgtgaa tcgcccgcct gagcgcgtcg
     3721 gcatagcggt tattggggaa gtacgcctct accgcgcgat gcttaaacac cacccccgac
     3781 gaaccgcgct cgatcaaaaa attggccacc gcatccaaac aagcggcgct ggtataaacc
     3841 gtcaaatgta accaagattc agtcacattt ttttatgcca cagcaccgac ggcgtagcaa
     3901 ttcttcgcgg cgtgttcccg ccgtcacagt cttcgatcta tcatttcgag gctcagcaaa
     3961 tcttatccac aatttatttc cgagctctcc gaccttggcg tcttggcgct tttgccggag
     4021 acaccctgag ttccgcttgc aatgctgcca cgcgtttagc atcgtcatct tgtcaaaata
     4081 atcgcttcca gatattgaaa tgaattgatg tgaaaggaac ttccgatgaa acttgccacg
     4141 atcaaacctg acggactcgc tattgttcac aacgacacgc tgattcccat caccggtgta
     4201 ttgcctaggg gatcaagcat gctcgacctg atcgcccaa
//
LOCUS       DATLCH010000047         3569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3885565, whole genome shotgun sequence.
ACCESSION   DATLCH010000047 DATLCH010000000
VERSION     DATLCH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3885565"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1378
                     /gene="yhbH"
                     /locus_tag="VMZ02_01280"
     CDS             176..1378
                     /gene="yhbH"
                     /locus_tag="VMZ02_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HUR70585.1"
                     /translation="MSTIFRPYNTSSGQRSDRSAGDRLRHRQKVREAIRNNIADIIAE
                     ESIIGKDHNRIIKVPIRGVKEYRFIYGDNTPGVGEGGDGDPKPGDVVGQGEDGKAGQQ
                     AGNRPGIDYYETDVSLDELIDLMFEDLVLPDLERKRLRKIEFERLIKQKGYRRKGIRV
                     RLDKKRTALSRVKRKKAMQRRQLARPQGLGESASSERFPFHKDDLRYHHTVTDTREES
                     NAVVLCIMDTSGSMDTMKKYLSRSFFFLLYQFISTRYQNVEIVFIAHHTEAKEVTEDE
                     FFHKGESGGTMISSGYIKALEIIEQRYHPALWNVYVFHCSDGDNFESDNPATLKAAQE
                     LAKAANLFGYGEIKPLGSGYYGSSLIGFFSQIQEPNFLAVQIQKKEDVWSSFKTFLTK
                     DRAKEDAA"
     gene            1387..2820
                     /locus_tag="VMZ02_01285"
     CDS             1387..2820
                     /locus_tag="VMZ02_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HUR70586.1"
                     /translation="MATDYNLDDLERWNERILELVQRFGLDHFDQEFEICDHEDMISY
                     MVYSGMPSHYPHWSYGKSFEKLMTLYDYGVSVLPYEMVINSNPSIAYLMRDNSLALQV
                     LTIAHVYGHNDFFKNNFTFRSTRAEFTIENFKSHANRVRHYVEDPSIGLERVESVVDA
                     AHALSLQCRRNLAVRKLTAHEERQRKIEEAKLPDDPFHAVHRRREIAEPDLQKVPLYP
                     DQDLLLFIRDRNPLLAEWEKDLLTIVHEEAQYFIPQIETKIMNEGWASFWHKRILETL
                     ELPQGLHLEFLVRHAQVLRPSPGSLNPYHVGMKIWEDIEKRWKNPSATEEKEFGPRKK
                     SANEKLFEVREVERDSSFLRRYLTEDLIRELNLFEYKNQGNQQVISHVADEDSWQHIK
                     DTLIRNVGTGTIPVIKVVDADYRNNRTLLLQHDHDGRDLQLEYAEKTLQYVQQLWGYD
                     VAMETVIDDQRTLLELSDRKLAIKKSA"
     gene            2887..>3569
                     /locus_tag="VMZ02_01290"
     CDS             2887..>3569
                     /locus_tag="VMZ02_01290"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70587.1"
                     /translation="MAAILSISEIHRLISNRLDEEAFANKTLAGIIGDAPSQYSKSPA
                     LWNAAFRHFAINAIYLPFDVDAVHLADLLRLLRDSEQFIGVNVTVPHKVRVIDFLDAL
                     DTGAARIQAVNTVVRDSSGKLTGYNTDGEGFVDSVLLPTPEHPEGFVSSLDGMDVLLL
                     GAGGSARAVAFHASDHIGKGRLIIANRTLAPARSLAAEIVKLGRRAIAIDEADVPIWA
                     PNAGLIVNAT"
BASE COUNT          883 a         1000 c          926 g          760 t
ORIGIN      
        1 cttgagccgg gtgatcacga aatcgcgcat gcgcgatgaa gagcagtcgg agaaatataa
       61 cgcgatggtc aagaatcttt tggagaacgg ctactgccag agctgtgtcg acgtcgtgct
      121 caagtatgcc gccaacaatc tttggaaaga ctaaggcggg agactctgcg tcgacatgtc
      181 aaccatcttt cgcccgtaca atacgtcctc cggtcaacgc agcgatcgca gtgccggcga
      241 tcgtctacgc caccggcaaa aagtccgcga agcgattcgc aacaatattg ccgacatcat
      301 cgccgaagaa tcgatcatcg gcaaagatca caaccgaatc attaaagttc ctatccgcgg
      361 cgtgaaggag taccgcttta tctatggcga caatactccc ggcgtcggcg aaggcggcga
      421 tggtgatccc aagccgggag atgtcgtcgg ccaaggtgag gatggcaaag ccggccaaca
      481 ggccggcaac cgtcccggca tcgactatta cgagaccgac gtcagcttgg atgagctcat
      541 cgacttaatg ttcgaggacc tcgtgttgcc ggacctcgaa cgcaagcgtt tgcgcaaaat
      601 cgagtttgaa cgattgatca aacaaaaggg ctatcgtcgc aaaggcatca gagttcgcct
      661 agacaagaag cgcaccgcgc tgtcacgagt caaacgcaaa aaggcgatgc agcgtcggca
      721 gctggcgagg ccgcaagggc ttggagaatc agcctcgtcg gagcgtttcc cctttcacaa
      781 agacgacctg cgctatcacc atacggtgac cgacactcgc gaggagtcca atgccgtggt
      841 tctgtgcatc atggatacct cgggttccat ggatacaatg aagaaatatt tgtcccgcag
      901 tttcttcttc ctcctttatc agtttatctc cactcgttat caaaacgtcg aaatcgtttt
      961 catcgcgcat cacaccgaag ctaaagaggt caccgaagac gaatttttcc ataagggcga
     1021 gtccggcggc acaatgatct cgtccggcta tatcaaagcg ctggagatta tcgagcagcg
     1081 ttatcatccg gcactgtgga acgtttacgt gtttcattgt tcggatggtg acaatttcga
     1141 gtccgacaac ccggcgacac tcaaagcagc acaagaactc gccaaggccg ccaatttgtt
     1201 cggctatggc gagatcaaac cgctcggctc gggctactat ggcagcagct tgattggctt
     1261 tttttcgcag atacaggagc ctaactttct tgccgtgcag atccagaaga aagaagacgt
     1321 ttggagttcc ttcaagacct ttctgaccaa ggaccgcgcc aaagaggacg ccgcctgaga
     1381 tacatcatgg ccaccgatta caacctggac gatcttgaac gttggaacga gcgcattctc
     1441 gagcttgtgc aacgcttcgg gctggaccat ttcgatcaag aatttgaaat ttgcgatcac
     1501 gaggacatga tttcctacat ggtctactcg ggtatgccgt cccattatcc gcattggtcc
     1561 tacggcaaaa gcttcgaaaa actcatgact ctctacgact atggcgtcag cgtcttgccc
     1621 tatgagatgg tcatcaactc caatccgtca attgcctacc tgatgcgtga caacagcctg
     1681 gcattgcagg tgctcacgat cgcccacgtc tacgggcaca acgatttttt caagaataat
     1741 ttcacctttc gctctacacg cgccgaattc accattgaaa atttcaaaag tcacgccaac
     1801 cgggtgcgcc attatgtcga agatccaagc atcggcctgg agcgggtgga atctgtcgtc
     1861 gacgcggccc atgctctgtc actgcaatgc cggcgcaact tggcggtgcg taagctcact
     1921 gcgcacgaag aacggcagcg caaaatcgaa gaagccaaac tgccggacga ccccttccac
     1981 gcggttcacc gccgacgcga gatcgccgaa cccgatctgc aaaaagtgcc gctctacccg
     2041 gatcaagacc tattgttatt catccgtgac cgcaatccgc ttctcgccga gtgggagaaa
     2101 gatctattaa cgatcgttca cgaagaagcc cagtacttca ttccacaaat cgagaccaag
     2161 ataatgaacg aaggttgggc gagcttctgg cataagcgca tcctggaaac tctcgaactt
     2221 cctcaagggc tgcatctgga gtttctcgtg cgccacgccc aggtgctccg cccctcgcca
     2281 ggcagtctca atccctatca cgtcggcatg aaaatctggg aggacatcga gaagcgctgg
     2341 aagaatccga gcgccacgga ggaaaaagaa ttcggcccgc gcaagaaaag cgccaatgaa
     2401 aaacttttcg aagttcgcga ggtcgagcgc gacagttcgt ttttgcgccg ctacctgacc
     2461 gaagatctga tccgcgaact taatctgttc gaatataaga accagggaaa ccagcaagta
     2521 atcagtcatg tcgccgacga agacagctgg cagcatatta aagacacgct gatccggaac
     2581 gtcggtaccg gcacgatccc ggtcattaag gtggtcgacg ccgattaccg caacaaccgt
     2641 acgctcttgc tgcaacatga ccatgacggc cgcgatctac agttggaata tgctgagaag
     2701 actcttcagt acgtgcagca actgtggggg tacgacgttg ccatggaaac tgtgatcgat
     2761 gaccagcgaa ctctcctgga gctctcggac cgcaaactcg ccatcaaaaa atcggcctga
     2821 gtgccggttc gcgttgcttg ccaaaaaaat agctgcgtgc gactacttgt tagcaccagt
     2881 gcggccatgg cagcgatact cagcatcagt gaaattcatc ggcttatctc caatcgtttg
     2941 gatgaagaag ctttcgccaa caaaacgctc gccggcatca tcggcgacgc tccttcgcaa
     3001 tactcaaaga gtccggctct gtggaacgcc gcgtttcgtc atttcgcaat caatgcgatt
     3061 tacttgccgt tcgatgtcga cgcggtccat cttgccgatt tattacggct gttgagagat
     3121 tccgaacaat ttatcggcgt caatgtcacc gtgccgcaca aagtgcgggt gatcgatttc
     3181 ctcgacgcgc tcgacaccgg cgccgcgcgc atccaggccg tcaacacagt cgttcgtgac
     3241 tcaagtggca aactgaccgg ctacaacacc gatggcgaag gattcgtcga tagcgtgctc
     3301 ctgccgactc cggagcaccc ggaaggtttt gtgtcctcgc tcgacgggat ggatgttttg
     3361 ctcttaggcg cgggtggctc ggcgcgcgcc gtggcatttc acgctagcga tcatatcggc
     3421 aagggtagac tgatcatcgc caatcggacg ctcgctcctg ctcgatctct cgccgcggag
     3481 attgtgaaac tcgggcgccg agccattgcc atcgacgaag cggatgttcc aatatgggcg
     3541 ccaaacgccg gactgatcgt gaatgccac
//
LOCUS       DATLCH010000048         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10516736, whole genome shotgun sequence.
ACCESSION   DATLCH010000048 DATLCH010000000
VERSION     DATLCH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10516736"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1055)
                     /locus_tag="VMZ02_01295"
     CDS             complement(225..1055)
                     /locus_tag="VMZ02_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR70588.1"
                     /translation="MLKPIGMTEGHYECRSLDETLPIFTELLAAEVIERSEKKAVVKH
                     PNTKWRLVIHEGGPDSKNKPHTNHYGFRVATPKEIPIAHQYISDHKAQYKIKSVTPPH
                     EAHFAYSIYFKEPGGNDLELEYYNPAAIKQGRRIAAPHWTTPLPEEKFPGKGYVPQAM
                     THGTLNCDDKETSDKFLANVLGLDIIGGGRTSTYISVPNMGPWYVVVLPTTHRDYLSE
                     ANRFTLQVASPEEVEDAHKEFAKTGKQLGIHELRELKTNGRAHFLLSDINKNWWEITS
                     "
     gene            complement(1116..2216)
                     /locus_tag="VMZ02_01300"
     CDS             complement(1116..2216)
                     /locus_tag="VMZ02_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70589.1"
                     /translation="MGEVVNRPSYQPVDSKVEIMDTPYDRWIASQGIDIVRGFFVADL
                     YTTPLKWWDRVGGNGVCIMLDGAGYMDDAYVIGIPPGKSLNPQRHIYEALVYVLSGRG
                     ATTVWQENGAKQTFEWQTGSLFALPLNAWYQHFNGQGDQETRLLSVTTAPLIFNLFRS
                     ADFVTNCPYSFTERFKGEEGFFTGGGKLYSDRVVETNFVADVVNIEPVEWAERGKGNA
                     TIFFEMSESMMGSHVSRFPVGLYKKAHRHGPGAHVFILTGQGYSLLWPEGKEMTRVDW
                     KPGSIVVPPEGWFHQHFNSGAEPSRYLALKILSRKYKLQPGKIQSDVPLAYGGWQIEY
                     EDEDPIIRKTFEEECAKSGAEVRMPTFAKAAN"
BASE COUNT          553 a          702 c          683 g          596 t
ORIGIN      
        1 gtcgttcggt gaatcaggca cacgtctgat cccaataaga tttctcgctt tgctcgaaat
       61 gacagccgaa taatcgatcc gtcatctcga atgaagtgag agatctcggc cttactgaat
      121 tactacaaaa ggcatacatc aaaaattccc agcggctaaa aacaaaaacg ccgctgacag
      181 cagcgctgcc agcggcgttt aatcgagtgc ctacgaaata aatcctacga ggtaatctcc
      241 caccagttct tgttgatatc gctcaagaga aaatgcgccc gtccgttggt cttcaattcg
      301 cgcagctcgt gaatgccgag ctgcttgccg gttttcgcga actctttgtg cgcgtcttcg
      361 acttcttcgg ggctcgcgac ctgcagcgtg aagcggttgg cctcggatag ataatcgcga
      421 tgggtcgtcg gcagcacgac cacgtaccac ggccccatgt tgggcacgct gatataggtc
      481 gaagtgcggc cgccgccgat gatgtcgagg ccgagcacgt tggcgagaaa cttatcgctg
      541 gtttctttgt cgtcgcaatt gagcgtgcca tgggtcatcg cctgaggcac atagcctttg
      601 ccgggaaatt tttcctccgg cagcggcgtg gtccagtgcg gcgcagctat gcggcgaccc
      661 tgtttgatcg ccgccggatt gtaatattcc agttccagat cgttgccgcc gggttcttta
      721 aagtaaatcg aataagcaaa atgcgcctca tgcggcggtg tgacgctctt gatcttgtat
      781 tgcgccttgt ggtcggaaat atactgatgc gcgattggga tttcttttgg ggtcgcgaca
      841 cgaaagccgt aatgattggt gtgcggcttg ttcttcgaat ccgggccgcc ttcgtgaatc
      901 accaaacgcc acttggtgtt cggatgcttg accactgcct ttttttcgct gcgttcgatt
      961 acttctgcag ccaacaactc ggtaaagatc ggcagcgtct catcgagcga gcggcattcg
     1021 taatgtcctt cggtcattcc gatcggtttc agcatgtttt ctccttaaac ctcaacgagc
     1081 tcacaagata ttattaggaa aggctctgac gcttgtcaat tggcggcttt ggcgaacgtt
     1141 ggcattctca cctcggctcc cgacttggca cactcttcct cgaaggtttt gcggatgatc
     1201 gggtcttcgt cctcgtactc gatctgccag ccaccgtagg cgagcggcac gtcggactgg
     1261 atcttgccgg gctgcaattt gtatttgcgg ctgagaattt tcagtgccag gtagcgcgat
     1321 ggctcggcgc ccgaattgaa atgctgatgg aaccagcctt ccggcggcac aacgatggaa
     1381 ccgggtttcc agtcgacgcg agtcatctcc ttgccctcgg gccaaagcaa cgaataaccc
     1441 tggccggtga gaatgaacac atgggcaccg gggccgtggc gatgggcttt cttgtaaaga
     1501 ccgacgggaa agcgcgagac atgcgagccc atcatgcttt ccgacatttc gaaaaagatc
     1561 gtcgcgttgc ccttgccgcg ctcggcccac tccaccggct cgatattgac gacatcggcg
     1621 acaaaattgg tttcgaccac gcgatcgcta tacaacttgc cgccgccggt gaagaacccc
     1681 tcttcgccct taaagcgctc ggtgaacgaa tacgggcagt tggtaacgaa atcggcgctg
     1741 cggaaaagat tgaagatcag cggcgccgtg gtcaccgaca acaggcgggt ttcctgatcg
     1801 ccctgaccgt tgaagtgttg ataccaggcg ttgagcggca gcgcgaacaa gctgccggtt
     1861 tgccactcga aggtttgctt ggcgccattt tcctgccaga ccgtcgtcgc gccgcggccg
     1921 gagagcacgt aaaccagcgc ctcgtagata tggcgctgcg gattgaggct cttacccgga
     1981 ggtatgccga taacgtaagc gtcgtccatg tagccggcgc catcgagcat gatgcacacg
     2041 ccgttgccgc cgacccgatc ccaccattta agcggcgtcg tatagagatc cgcgacgaaa
     2101 aagccgcgca cgatatcgat accctgggat gcaatccagc gatcgtacgg cgtatccatg
     2161 atctcgacct tgctatcgac tggttgatag gaaggacgat tcacaacttc tcccattggc
     2221 tgcctcctga aaattatcta acttttttta tcggtattcg cccgccgcat caaccctcgg
     2281 ggtaaaaccg aggggttgaa ttcctcgatc agaagtcgcc atcctgatcg tcattccggc
     2341 ggaggccgga atccaggctt tctttggctt tgtagcacaa ccgaagatgg atcccctaaa
     2401 ttcacaaacc aagtgcaaca cttattggat aaggtgttgc catgggacaa aactatgaac
     2461 agttttcgct tgaagagcgc tgtacacttt cccgacttag ccaagccggg aaaacaatcc
     2521 gccaaatcgc ggca
//
LOCUS       DATLCH010000049         2727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6045812, whole genome shotgun sequence.
ACCESSION   DATLCH010000049 DATLCH010000000
VERSION     DATLCH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6045812"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VMZ02_01305"
     CDS             complement(<1..653)
                     /locus_tag="VMZ02_01305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUR70590.1"
                     /translation="MIPYRDENETQRPAIVTGIIIGINILVWLVVQGAGADLPLAESV
                     CNLGLIPGELTGLQPPGRGFPIGPGVACVTDPGRQASHLITSMFLHGGWMHLIGNMWF
                     LWIFGNNVEDSMGHLRFVAFYLLTGLAAALGQVITSPDSLVPMVGASGAISGVMGAYL
                     VLYPRVRVYAFIPIFIFFTSIALPAWAMLGYWFVLQLLSGLFSGGEMGGVAFWAHIGG
                     "
     gene            950..1261
                     /locus_tag="VMZ02_01310"
     CDS             950..1261
                     /locus_tag="VMZ02_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70591.1"
                     /translation="MLDIKTKNEEGISKTSTTQVQSPATGQAAIVLHDLTTGPEAEAN
                     RLKTRVSELTAENGNLRNENQSLRAELREIKLVLSKVQIDELRKFEKVNSWLRSFKKM
                     T"
     gene            1493..2587
                     /locus_tag="VMZ02_01315"
     CDS             1493..2587
                     /locus_tag="VMZ02_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUR70592.1"
                     /translation="MSTKGRAAIYVEAKKPLVVDEVEFGDPGPDQVLVKLFASGVCHS
                     QLHTMQRPARKGQSLPALLGHESTGVVVARGSDVRHVKEGDHVITTWVDRDSATTNQP
                     MVAHALNDRAQYNADWKGKAVMHSAATWAEYAIAQERVVVPMPKDLPTDVTSIIGCAV
                     MTGAGAIVNTLQVRPGQSVAVFGAGGIGLCAISAAAVVDAYPIIAVDINQDKLEFAGR
                     MGASHAINAKDCDAVEAIKEVTGGGVDYAIDAIGLPLTQEQILRSVRPGFSGLNQGGT
                     ALLIGITPPGAKALLDTSLFIGNRSFKRTSGGDCKPDRDFPTFLRWFRDGKLKLNELI
                     TNRYKLEQINEAVDDLERGRILGRAILAFD"
BASE COUNT          653 a          745 c          776 g          553 t
ORIGIN      
        1 ccgccgatat gcgcccagaa cgccacgccg cccatctcac cgcccgagaa cagaccgctc
       61 aacaactgaa ggacaaacca ataaccaagc atcgcccacg ccggcagcgc gattgacgtg
      121 aaaaagatga atatcggtat gaacgcatag actctgacgc gcgggtaaag caccaggtac
      181 gcgcccatga cgccgctgat cgctcccgag gcgccgacca tgggcaccag cgagtccggg
      241 ctggtaatta cctgtcccaa cgctgccgcc aagcccgtaa ggaggtaaaa cgccacaaag
      301 cgcagatgcc ccatcgaatc ttcgacatta ttgccgaaga tccacagaaa ccacatgttg
      361 ccaataagat gcatccagcc gccatgaaga aacatcgagg tgataagatg ggaggcctgg
      421 cggccgggat cggtcacgca ggctacaccc ggcccgatcg gaaagccccg tccgggtggt
      481 tgtaaaccgg tgagctcgcc gggaataagc ccgagattgc acaccgactc ggccagcggg
      541 aggtccgcgc cggccccttg cactaccagc cacacgagga tgtttatccc gatgattatt
      601 ccggtgacga tggcggggcg ctgtgtttca ttttcatcgc ggtaggggat catagatatt
      661 tcctagcgca attttgggca aaagccgaga ggctattttt cacgggcggc ggcgtgcaca
      721 gatttgttgt agtcaaaaat ttcaagagct atcggaagtg caattcggta cgcttcggca
      781 attctcaacc gagaagttca tggtcgtgca agcggttaaa accagctgtg taaaaatttg
      841 ctctgcttga cgcaagcgcg tcataaacga ctttgcggcc agtgccgaaa tttgccactg
      901 ccgatggccg acgctttgct cctatgtcgc gtagggggag ataagcgcca tgttagacat
      961 taaaacgaaa aatgaggaag gtatctcgaa aacgtccact acgcaggtgc agtcgcccgc
     1021 gaccgggcaa gctgccatcg tcctccacga tctcacaacc ggccccgagg ccgaagccaa
     1081 ccgcctaaag acccgggtga gcgagttgac cgcggaaaac ggcaatttgc gtaacgaaaa
     1141 ccaaagtctc agggccgaac tgcgggaaat caagcttgtg ctaagcaagg ttcagattga
     1201 cgaacttcgc aagttcgaga aggtcaattc ttggctgcgc tcgttcaaga aaatgacttg
     1261 atctttgtaa gtctctgata gtttcgttta acgctactcc actccgcatt ttttacgcgg
     1321 tttttcctcc ctgaatcgag cgaattggcg attcgtcact ccgcggtggt gctccaaaag
     1381 ctctggacaa actgccgtat tcaccataaa tagccggtgt gaatacacga cgtagtttgc
     1441 ttcgcgcaag cggcgctttc gcctctttgt taatttgcgg catggaggcg acatgagcac
     1501 gaaaggccgg gcggccatct atgtcgaagc gaaaaagccg ctagtggtcg atgaggtgga
     1561 gttcggcgat cctggacccg atcaggtttt ggtcaagttg ttcgccagcg gtgtctgcca
     1621 ttcccaacta cacactatgc aacggccggc acgcaaggga cagagcttgc cggcgttgtt
     1681 gggccacgag tccactggcg ttgttgttgc cagaggaagc gatgtgcgtc atgtgaagga
     1741 aggcgatcat gtcattacta cttgggtcga tcgtgatagc gccaccacca accagccgat
     1801 ggtagcccac gcgctgaacg accgcgctca atacaatgcc gattggaaag gtaaggccgt
     1861 catgcactcg gcggccacct gggctgaata cgccatcgcg caggagcgcg tcgttgtgcc
     1921 tatgccaaaa gatctgccga ccgacgttac ttcgatcatc ggctgcgcgg tgatgacagg
     1981 ggcgggtgcg atcgtcaaca cgctgcaagt gcggccaggc caatcggtag cggtgtttgg
     2041 cgcgggcggt atcgggctat gcgcgatttc cgcggctgcc gtcgtcgatg cttacccaat
     2101 tatcgccgtc gatattaacc aagataaact agagtttgcc gggcgcatgg gtgccagtca
     2161 tgcaatcaat gcaaaggatt gcgacgctgt agaagcgatc aaagaagtga ccggcggcgg
     2221 tgttgattat gcaatcgacg ccatcggctt gccgctgact caggaacaga tcttgcgctc
     2281 ggtgcggccg ggattttccg gactgaatca aggcggaacc gcattactga tcggcattac
     2341 gccccccggc gcgaaagcgc tgctcgacac cagcttgttc atcggcaatc gctcgttcaa
     2401 acgcaccagc ggcggcgact gcaagccgga tcgcgatttt ccgacgtttc tgcgctggtt
     2461 tcgcgatggc aaactgaagc tgaacgaatt gatcaccaac cgctataagc tcgagcagat
     2521 caacgaagcg gtcgacgatt tggaacgcgg ccggatattg ggacgggcaa ttttggcgtt
     2581 cgattgaaaa tggattggcc gcaaagaacg taaaagaaat cggcaggtac gtgagaccgg
     2641 gacgcccgcc ctgtcatttc gaccgacaga gtgtgtgaaa aaatcagcgt ctctctgatc
     2701 tgccatctcg aacgcgtgag agatctt
//
LOCUS       DATLCH010000050         3501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8280448, whole genome shotgun sequence.
ACCESSION   DATLCH010000050 DATLCH010000000
VERSION     DATLCH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8280448"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VMZ02_01320"
     CDS             complement(<1..395)
                     /locus_tag="VMZ02_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUR70593.1"
                     /translation="MFVKANKIGMFSGLFSAIFSLLTVFSQPAGAQGKLVIAIQPTVA
                     SDEMLSKAKPLQNFLEQSLGGKTKVEIYVPSSYAAVVESLRFGHAQMAFMSAWPAQLA
                     VRLGGAEVALAEVRQVLHGKEKVDAPYYYS"
     gene            537..1202
                     /locus_tag="VMZ02_01325"
     CDS             537..1202
                     /locus_tag="VMZ02_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HUR70594.1"
                     /translation="MEEPKVTHALEDYLKAIYVLMEDGQAVIAARMAAEAGVSASTIF
                     ATVRRLEKDGYVTINRRKEINLTSEGKNIAERTIRRHFLTERFLTDLLGLDWVKAHQE
                     AHRLEHAISQEVEERLAKLLNNPLTCPHGNRIPGANAGNLIKTMPLNDASDGQTVELD
                     FITEGGERDVRLLAYLQEHGLIPGARVLVVDVAPSLGMMTLKVGDDEFSLGIEAAKKI
                     RVH"
     gene            complement(1219..1509)
                     /locus_tag="VMZ02_01330"
     CDS             complement(1219..1509)
                     /locus_tag="VMZ02_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70595.1"
                     /translation="MQRPKNRQEARLQDLIVSAKQLNIHVRMEKLLREVGYRAHSGRC
                     RVKGQELIVIDRDIPLIDQIEFLTNELAARRTEENSHPLAPANQVSEQTDSN"
     gene            1698..2534
                     /locus_tag="VMZ02_01335"
     CDS             1698..2534
                     /locus_tag="VMZ02_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUR70596.1"
                     /translation="MIKNILIPLDGSEHAKAALDYAIWLARGFDGMLLGQHVIDTVSL
                     EGTFFHDVSGSLGFEPYLDFSTKMRELLDERGKLILDNFRETCAANNIRAQTFLDTGL
                     IANEICERANVADLVVIGHRGVNEGFTAGMLGSTAETIIRKCPRPIFVSTQRFEKIAN
                     PLLAYDGSQRASSAMESAAEFCTVLKLPLTVLTIAREEAAAQAILQQAKAYLGSYAID
                     VQYEIARGYPEQKIIEYLVNLKYDLLFIGAHGHRRIIKMVLGSTTEYVLRNSPCPVFL
                     NR"
     gene            2571..3389
                     /locus_tag="VMZ02_01340"
     CDS             2571..3389
                     /locus_tag="VMZ02_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HUR70597.1"
                     /translation="MKFVAAAVQMQASDDKASNLAEAERWVHQATRQGARLVVLPEVF
                     IWRGNKKLEREFAEIIPGATSNRLSGLARQLSIFLLAGSILEEIPASSKAYNTSLLFD
                     PSGNLLATYRKIHLFDVDLANGVSLRESETRSHGEALVAAQTELGTMGLSVCYDLRFP
                     ELYRGLADQGAQLIFVPSAFTAFTGQAHWEPLLRARAIENQSYIIAADQFGKSAKSFE
                     CHGHSMIVDPWGTILAELPDGPGVITAEIDLDHLAKVRAELPALQHRRILNNPS"
BASE COUNT          809 a          907 c          954 g          831 t
ORIGIN      
        1 gaataatagt agggagcgtc gactttttct ttgccatgga gcacctggcg cacctcagcc
       61 agagcaacct cggcgccgcc gaggcgcact gccaactgag ccggccacgc cgacataaat
      121 gccatctgag cgtggccgaa gcgcagcgac tccaccactg cggcgtagct gctcggcaca
      181 tagatctcca ctttggtctt gccgccgagg ctctgctcga gaaagttttg cagcggctta
      241 gctttgctca gcatctcgtc gctggcaact gtcggttgga tggcgatgac gagttttcct
      301 tgagcgccgg ccggttgaga gaaaactgtc aacagcgaaa agattgcaga aaacaggcct
      361 gaaaacattc ctatcttgtt ggctttaacg aacattcaat gctcccttga tctcctggca
      421 caacttgatt attaaggtgg caggcaaaaa ctagtttaga cattaattaa atccatgctt
      481 cgctatccga aagacctgag tttcgtatag tcgaagctag aaagtttgtc aaacctatgg
      541 aagagcccaa agtcacccat gcgttggaag attatctcaa agctatttat gtgcttatgg
      601 aagatggaca agcggtcata gccgcgcgca tggctgcgga ggctggggtt tcggcgtcga
      661 cgatcttcgc caccgtccgg cgtttagaaa aagacggcta cgtgacaatc aatcgtcgca
      721 aagaaatcaa tctgaccagt gaaggtaaga atattgcgga acgaacaatt cgccgccatt
      781 ttttgaccga gcgatttttg actgacctac tagggttgga ttgggtgaag gcacatcagg
      841 aagcgcatcg attggaacat gcgatctccc aagaagtcga agagcgcttg gctaaattgc
      901 taaacaatcc gctgacctgt ccccatggca atcgcatacc cggagccaat gcaggcaacc
      961 tgatcaaaac tatgccgcta aacgatgcca gtgacggtca aaccgttgag ctcgatttca
     1021 ttaccgaagg cggtgaacgc gatgtacggc tattggctta cttacaggag catggactga
     1081 tcccgggagc tcgggtgtta gtcgtcgatg tcgccccatc tctcggtatg atgacgctaa
     1141 aagttggcga tgatgagttt tctttgggaa tcgaggcggc aaaaaagatc cgggtgcact
     1201 gattcagttt gctgcgggct aattagaatc agtttgctcg gatacttgat tggctggggc
     1261 gaggggatgg gagttttcct cagttcggcg cgcggcgagt tcgttggtga gaaattcgat
     1321 ctgatcaatc agcggaatat cgcggtcgat aacgatcagt tcctgtcctt tgactcggca
     1381 acgcccgctg tgggcacgat aaccgacttc gcgtaaaagc ttttccatgc gcacatgaat
     1441 attcagctgt ttggcgctga caattagatc ctgaaggcgg gcttcctggc ggttttttgg
     1501 tcgctgcatg tgtccaactt ctctagagta ggccggatta tctcctaaag acgcacagtt
     1561 gtaaacgctc ggggtggcgg attccgtttg taattgtttt cgcccttgtt atcgtaccgt
     1621 tggcatctat atacctgtat atcaatgagg ccagtcgcgg gtggtatgat cgcgccgaca
     1681 agtgaacgga gggacgtatg attaaaaaca tcctgattcc tctcgatggc tcggagcatg
     1741 caaaggccgc gctcgattat gcgatttggc tggcgcgcgg cttcgacgga atgttgttgg
     1801 gccaacatgt gatcgatacg gtgtcgctgg aaggaacctt cttccacgat gtttcgggtt
     1861 cacttgggtt tgaaccatat ctcgatttct cgacgaagat gcgcgagctc ctggatgaac
     1921 gcggcaaact tattctcgat aactttcgcg agacttgcgc ggccaacaat attcgggcgc
     1981 aaacctttct cgacaccgga ctgatcgcca acgaaatctg tgagcgcgcc aacgttgcag
     2041 atctggtggt gatcggtcat cgcggcgtaa acgaaggttt taccgccggc atgctcggtt
     2101 caaccgcgga aacgatcatt cgcaaatgtc cacggccgat tttcgtttcc acccagcgct
     2161 tcgagaagat tgcaaacccg ctgctggcat atgacggcag tcagcgcgcc agctcggcga
     2221 tggaatcggc ggccgagttt tgcacggtgc taaaactgcc gctcaccgtc ctgaccatcg
     2281 cccgagaaga agccgcggcg caggcaatcc tgcaacaggc taaagcctat cttggctcct
     2341 acgccatcga cgtccagtat gagattgcgc gcggctaccc cgagcagaaa ataatcgagt
     2401 atctggtcaa tcttaaatac gatttactct tcatcggtgc tcatggccat cggcgcataa
     2461 tcaagatggt gcttggcagc acgaccgaat atgttttgcg caattcgccc tgtccagtat
     2521 tcttaaacag gtagcagcca ttcaaagttc aacctggcaa tgccgagaat atgaaattcg
     2581 ttgccgccgc ggtgcagatg caggcttcag atgacaaggc gtcgaatctc gccgaggctg
     2641 aaaggtgggt gcaccaggcg acgcgccaag gggcgcgact cgtggtcctg ccggaagtgt
     2701 tcatatggcg cggcaacaaa aaattggaaa gagaattcgc tgaaattatt cccggagcga
     2761 cttccaaccg actcagcggg ctggcgcgac aactaagtat ttttctgctc gccggttcga
     2821 tcctggaaga gattcccgcg agttccaaag cctacaacac gagcctgctc tttgacccgt
     2881 ccggaaatct tctcgcaacg tatcgtaaga ttcatctttt cgacgtggac cttgccaacg
     2941 gcgtatcgtt gcgcgagtcg gagacccgct cgcacggcga agcgctcgtg gcggcacaaa
     3001 ctgagctagg tacgatgggt ttgtcggtgt gttatgattt acgcttcccc gaactgtacc
     3061 gcggtttggc cgatcaaggc gcgcaattga tcttcgttcc gtctgcgttt acggccttta
     3121 ccggccaggc gcactgggaa ccgctattgc gcgcccgtgc catcgaaaat caaagctaca
     3181 tcattgccgc cgatcaattt ggtaaaagcg ccaaaagctt cgaatgccat ggccattcaa
     3241 tgatcgtcga cccctggggc actattctag cagagctgcc tgacggtccc ggtgtgatca
     3301 cggcggagat cgatctcgat catcttgcta aagttcgtgc cgagctgccg gccctgcagc
     3361 accggcgcat tctgaacaac ccttcgtagt cgccgtcgca gcacgttttt cctgggtaca
     3421 tacgagcaaa tccaaatgga attcgtggag ccttgattag ggttcatgca atgctgtgag
     3481 ctggcggatc cgcgtggact t
//
LOCUS       DATLCH010000051        19023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_169284, whole genome shotgun sequence.
ACCESSION   DATLCH010000051 DATLCH010000000
VERSION     DATLCH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19023
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169284"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1531
                     /gene="rho"
                     /locus_tag="VMZ02_01345"
     CDS             206..1531
                     /gene="rho"
                     /locus_tag="VMZ02_01345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HUR70598.1"
                     /translation="MVRGRTRSAEPETVEEVVKEVVKDNGLNLKALKEKKIGDLALIG
                     KTFNIEGAANMRKQDLIFAILQAQTEQNGHVYGEGVLETLPDGFGFLRAPDYNYLPGP
                     DDIYVSPSQIRRFSLRTGDIISGHIRPPKEGERYFALLKVETINYEDPERAREKILFD
                     NLTPLYPNERLCLENQPTDYTTRIVDLLTPIGKGQRGLIVAAPRTGKTMMLQHIAKAI
                     AHNHQEVVLIVLLIDERPEEVTDMQRSVDGEVISSTFDEPATRHVQVAEMVIEKAKRL
                     VEHGKDVVILLDSITRLARAYNTVVPPSGKILSGGVDSNALHKPKKFFGAARNIEDGG
                     SLTIIATALIDTGSRMDEVIFEEFKGTGNMEVHLDRRLMDKRVFPAIDINKSGTRKEE
                     LLIPREDLNRIWILRKVLSQLSVVESMEFLLDKMHGSKTNKEFLESMNQ"
     gene            1760..2530
                     /gene="rpsB"
                     /locus_tag="VMZ02_01350"
     CDS             1760..2530
                     /gene="rpsB"
                     /locus_tag="VMZ02_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HUR70599.1"
                     /translation="MAEISMKQLLEAGVHFGHQTSRWNPKMKPYIFGARNGIHIIDLQ
                     QTVSMFKELEGVVRDLAASGGHVLFVGTKKQAQDAIREEATRCGMYYVHNRWLGGTLT
                     NFTTIRQSIERLRKIEEMEADPKIVEALTKKEMLGLHREKDKLEQSLGGIKAMRKLPD
                     AIFVIDPKQEEIAVKEARKLGVPVIAVIDSNCDPDMIDYKVPGNDDAIRAIRLFCQAI
                     AEAVIEGRTIYEQSLVKDKADDRPAEAVFAAESAAGGV"
     gene            2536..3144
                     /gene="tsf"
                     /locus_tag="VMZ02_01355"
     CDS             2536..3144
                     /gene="tsf"
                     /locus_tag="VMZ02_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HUR70600.1"
                     /translation="MEVTASLVKELRDKTGAGMMDCKKALAETGGDLQKAIDYLRQKG
                     LAAAAKKADRVASDGAVGAYVHAGGKIGVLVEINCETDFVARTAEFQNLLKDIAMQVA
                     AANPRCVRREEVTAEEFEREKIIYRQQALDSGKPEKVVEMIVQGKMEKFYSEVCLMEQ
                     AFIKDPDKKVGDIVNEAIARLGENIQVRRFARYHLGEGGAKD"
     gene            3164..3895
                     /gene="pyrH"
                     /locus_tag="VMZ02_01360"
     CDS             3164..3895
                     /gene="pyrH"
                     /locus_tag="VMZ02_01360"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HUR70601.1"
                     /translation="MNNGGPKYKRIILKVTGEVFAGTGNFGIDGATVKAFAQEIKEVK
                     DLGCELALVIGGGNIFRGAVASEIGMDRASADTMGMLATVINSLALQDALEKIGVSTR
                     VLSAIEMRQVAEPYIRRRATRHLEKGRVVIFAGGTGNPYFTTDTTASLRAMEVSAEVI
                     LKATKVDGIYDADPLKRSDANKFEELTYIEVLNRELKVMDSTAISLCMDNQLPIIVFN
                     IMEKGNIKRVVSGESIGTLVSGGKR"
     gene            3892..4449
                     /gene="frr"
                     /locus_tag="VMZ02_01365"
     CDS             3892..4449
                     /gene="frr"
                     /locus_tag="VMZ02_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HUR70602.1"
                     /translation="MNEAIFTQVQKEMDGSVAVLRKDLTKLRTGRASTSLLEHITVDY
                     YGASTPLNQLATLSAPEPRLLVIQPFDRAAMTEIEKAILKSDLGLTPNNDGKIIRIPI
                     PALTEERRKELVKHVKKIAEEYRVSVRNHRRVAVEQIKEAEKKKELTADETKHGLDRV
                     QKITDDFIGRIDKIVKAKEDEVMAI"
     gene            4552..5331
                     /locus_tag="VMZ02_01370"
     CDS             4552..5331
                     /locus_tag="VMZ02_01370"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HUR70603.1"
                     /translation="MDLGGLDKSRLPQHVAIIMDGNGRWATQRRKHRIEGHRRGKTSV
                     RVIVEMSRRIGIRYLSLYAFSTENWLRPEDEVEALMGLLEHYLIAEQTKMMRYGIRLL
                     AVGNRNRLPAQLRRTLESVIEATAKNQRMTVILALSYSGRDDIVRMARVLAQQVSAGN
                     CQADCVDEQWISSHLDTASVPDPDLLIRTSGEMRISNFFLWQIAYSELYVTPTLWPDF
                     REKEYLQALLDFQRRRRRFGRTDEQMAEKPLVIMEAGGRKS"
     gene            5315..6121
                     /locus_tag="VMZ02_01375"
     CDS             5315..6121
                     /locus_tag="VMZ02_01375"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HUR70604.1"
                     /translation="MDGNLKARIGTAAVGLPLLVWLVGWSAQWLFSLVFFLLTVAALR
                     EYFAMICPGEFWQQVWGIVFGSAIGLTVLASPASVMALWLSVFLIILFASTLCSGGAL
                     IDRLNRLGWTLLGGLYIGFLVPQLALLFEFRDGRRWVAFVFLVIMAGDTSAYFIGRRF
                     GRRKLAPELSPGKTVEGSIAYVIGSILIGMLAGVLLALPVALLEVALVALVMSLLGQI
                     GDLFESLIKRAFAVKDSGELLPGHGGVLDRLDSLIFPVVFATFYLTVIHP"
     gene            6118..7266
                     /gene="dxr"
                     /locus_tag="VMZ02_01380"
     CDS             6118..7266
                     /gene="dxr"
                     /locus_tag="VMZ02_01380"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HUR70605.1"
                     /translation="MRTIALLGSTGSIGVSTLSLAREFPERFKIHGMVAGRNLQLLAS
                     QIKEFSPALVAIEREDDIAQLRKFLGKHKVDILSGQAGAIEVATASKVDVVLAAIVGG
                     AGLMPTLRGLLAGKEIALANKEALVMAGELFIKAAKKKGVRLLPVDSEHSAIFQCLQG
                     NQRGDVDKIILTASGGPFLRTPLQRLANVTIDQALKHPNWKMGRKITIDSATMMNKGL
                     EVVEARWEFDMEPSGIQVVIHPQSIVHSMVRYQDGAIMAQLGIADMRIPIAYALAYPH
                     RLKGNWEPLDLTKHKGLNFLPVEKKRFPGLSLAYSAIEEGGTMPAVLNAANEVAVAAF
                     LAGRIGFREIHRIIDKTMQRHQSKHAKNIDEVIEVDRWAREVATTEVH"
     gene            7289..8371
                     /gene="rseP"
                     /locus_tag="VMZ02_01385"
     CDS             7289..8371
                     /gene="rseP"
                     /locus_tag="VMZ02_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HUR70606.1"
                     /translation="MEGFLYSIGSAVIGLGLLIVFHEFGHFLLAKLSGVGVLTFSVGF
                     GPKILVRKKGETEYALSAFPLGGYVKMIGEDPNEEVSAADRERSFAHKSLLKRFAIVF
                     AGPAFNLILAVILLMLVFVFYGVPVMSTLVSGVEKGSPAERAGITKGDRIVAIDGKPV
                     VEWENLSQTIKSSEGRPLNLEIRRGSELVKLTVQPTRKEGRNIFGERKDDWLIGIGSQ
                     VSIEKGKPGLAVGRAFSQTWDYIKITVLGFYKMIMGDVSPKNIGGPILIAQMAGQQAQ
                     EGVGSFLAFLAVLSINLGVLNLLPVPVLDGGHLLFFLVEAVIRKPVSIRVREMAQQVG
                     ICLLGLLMVYAFYNDIVRFFEKQVGG"
     gene            8368..9117
                     /gene="tsaB"
                     /locus_tag="VMZ02_01390"
     CDS             8368..9117
                     /gene="tsaB"
                     /locus_tag="VMZ02_01390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HUR70607.1"
                     /translation="MRILGIDTATAVSSVAFFEDGQLTAEATFPPRNRTGEVLSVKSS
                     HSETILPLISSVLDNAHRKISDIDVIAVSIGPGSFTGLRIGLALVKGIAYECSVPVVG
                     VSSLEAQAARFTKISGTICSLLDARKHEVYAALFEQREGSLLRCSADQATEVTDLMAL
                     FSSSLRTSVIKFVGNGAELYQEQILHLFGSRAQIIGEACGCSAAGGVARVASTRSPAI
                     SRTDLGPLVPLYLGTSQALGISRKILSNSLK"
     gene            9205..9444
                     /locus_tag="VMZ02_01395"
     CDS             9205..9444
                     /locus_tag="VMZ02_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70608.1"
                     /translation="MEHSEELAIVSLLDKDPELKRYYDEHQAFEKQLAALHHKGHLSV
                     DEELEIKRIQKLKLAGKDKMMELLGKHRQFRDRSS"
     gene            9454..11232
                     /gene="ilvB"
                     /locus_tag="VMZ02_01400"
     CDS             9454..11232
                     /gene="ilvB"
                     /locus_tag="VMZ02_01400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HUR70609.1"
                     /translation="MKITGAEIFCESLRREGVKTIFGLPGGVVLKIFDVLCQRKDLQL
                     ILTRHEQGAAHMAEGYAKATGKAGVVLVTSGPGMTNIVTGLADAHMDSVPLVAFTGQV
                     STDLIGNDAFQEADNVGISRPCTKHNVLVKDVADLAQAVKEAFYIATSGRPGPVLVDI
                     PKDVSIAKTEFKYPDKVNLRGYNPFTGGDTSQIKHAAEAIMTSKRPIIYVGGGAVFGD
                     AADEVRELAEITQIPVTMTLMGLGTFPGTHELSLGMLGMHGGYWSNMAMHESDLLIAV
                     GARFDDRVTGKLSEFCPGARVIHIDIDPTSIRKTFHAHIPIVGDVKSVLRQMNVTLRS
                     LDGNPTAMKARRAPWLKQINAWKKEHPLSYKQDDKITKPQYVVEKLYELTKHEAIVAT
                     DVGQHQMFAAQYFKGKKPRTWLTSGGLGTMGFGFPAAIGAQVAYPDRQVVCITSEGSF
                     QMNLQELVVAAEHKLPVKIVLLNNGVHGMVRQWQDLFYESRYSASILGKMPDFVKLAD
                     AYGIKGLRAVKPSEVDGVLKEGLKHKGPVLMDIHTDPFENCYPMIPAGGAQHEMMLED
                     PPELKLTRKGGVHKRKVDEGDGVLPA"
     gene            11244..11765
                     /gene="ilvN"
                     /locus_tag="VMZ02_01405"
     CDS             11244..11765
                     /gene="ilvN"
                     /locus_tag="VMZ02_01405"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HUR70610.1"
                     /translation="MEHIISVLVENKFGVLARVAGLFSARGDNIESLSVAPTLDPTTS
                     MITIVTVGDDRIIEQIMKQLNKVVEVLSVIDHTETDFLDRETVLIKVNTKPEHRDEAI
                     RIAEIFRAKIVDSSPTTYTIEITGDVDKVEAMIGMLKPLGIKDLIRTGRIAISRETTR
                     TASPRREPALLKP"
     gene            complement(11866..12156)
                     /locus_tag="VMZ02_01410"
     CDS             complement(11866..12156)
                     /locus_tag="VMZ02_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70611.1"
                     /translation="MKYVKQLVLGAIATALLVTSAACDNVEKQNPGPAEKAGAAVGQA
                     IDQATEKAGKAMEKAGDAMKDVGEKAKEFGKDVIGKTKEAGKEAADKIKDTK"
     gene            12460..13476
                     /gene="ilvC"
                     /locus_tag="VMZ02_01415"
     CDS             12460..13476
                     /gene="ilvC"
                     /locus_tag="VMZ02_01415"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HUR70612.1"
                     /translation="MQVYYDRDADLNVLKSKKVGVLGYGSQGHAHANNLRDSGVDVVV
                     GLRRGSSSWAKAENAGLKVAETAEAAAIADIVMVLLPDELQGDAYESEIRPGLKNGNY
                     LAFGHGFNIHFKRIVAPAGVNVFMVAPKGPGHLVRSEYEKGRGVPCLLAVHQDPTGDT
                     TKIGLAYASAIGGARAAVIQTTFKDETETDLFGEQSVLCGGLCALIQAGYETLVDAGY
                     PPEMAYFECVHEVKLIVDLIYEGGMANMRYSISNTAEYGDLTRGKRVIGPEVRQAMQK
                     ILKDIQSGQFAEEWINEYKSGAPHFTELRKEQANHPLEKVGAGLRSMMPWLGHNRLVD
                     RSKN"
     gene            13518..14147
                     /locus_tag="VMZ02_01420"
     CDS             13518..14147
                     /locus_tag="VMZ02_01420"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HUR70613.1"
                     /translation="MRIAKEGWPLILPAAAVALMALLLGWVVVGVALAVLTIAIAAFF
                     RDPERKIPTGDGLVVAPADGRIVAIAPVEGNALFADAVTRISIFLSPLDVHINRAPVA
                     GRVSAVRYQAGKFIAAFKEEATRYNEQNALHMVDERERNLGVVQVAGVVARRIVCRAK
                     AGDILSRGERFGLIMFGSRTDIYLPQSCRLEATEGQRVTGGETIIGRFP"
     gene            14144..15004
                     /gene="pssA"
                     /locus_tag="VMZ02_01425"
     CDS             14144..15004
                     /gene="pssA"
                     /locus_tag="VMZ02_01425"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HUR70614.1"
                     /translation="MSAMNAPEPERPQEKDKLRLLRRRRERPRMTLRQRMPAEKLRKG
                     VYLIPSLFTAGNLMCGFFSIIATFRGEYVNAALLILLANVFDGVDGYVARLTRTTSQF
                     GVEFDSLADVVAFGVAPAVLVYIWALVPWQNWGWLAAATYVVCGAARLSRFNVQAQGP
                     AKNYFVGLPIPAAAQMITSSVILYYYLGFEGAPTRHLTFLLLIYGLAILMVSSIPYFS
                     LKNNDMRKRHPEWMFVSGVILITLIIAERHIMFFTIVLLYTLSGPWLWCLTNLKHRRE
                     LRREAAKVLS"
     gene            15107..16672
                     /locus_tag="VMZ02_01430"
     CDS             15107..16672
                     /locus_tag="VMZ02_01430"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HUR70615.1"
                     /translation="MDHMAKDNVVQIFDTTLRDGEQSPGASMTVEQKLVIARQLEKLG
                     VDVIEAGFAASSDGDFESVRRVALEVSEPRVVSLARAQESDISRALKAVEGAKKPGIH
                     TFIATSDIHLKHKLRMTREQVVEAAVKAVTYAKQFTDYVEFSAEDASRSDPEFLLQIF
                     REVIRAGAKTLNVPDTTGYAIPSEFGALIANLIERTAGGDRVIWSAHCHNDLGLAVAN
                     SLAAVHNGARQIECTINGIGERAGNTSLEEVVMALRTRKNYLDLDTNIHSEQLYPTSR
                     LLSQVVGIPIPINKPIVGDNAFAHEAGIHQDGVLKHKQTYEIMTPESIGIPGNRLVMG
                     KHSGRHAFDERLKHLGFNLSKEDMNRAFVRFKELADKKKNVYDEDIEAIVAEEVLRMP
                     GRPDKFEFLYMSVNSSSDGVPTATIKMRVDGAEKMDHASGDGVVDACYKVITKITDSQ
                     SQLVRYSVNAITGGTDAQGEVACLIDDGGIRVSGQGSHTDIIMASALAYINALNKLEE
                     RKRYRQVVEKEGP"
     gene            16669..17763
                     /gene="leuB"
                     /locus_tag="VMZ02_01435"
     CDS             16669..17763
                     /gene="leuB"
                     /locus_tag="VMZ02_01435"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HUR70616.1"
                     /translation="MNYNIAVFPGDGIGPEVMSEGTAVLKQVAELYGFTVNLESGMVG
                     GASIDAHGKPLTDAVLSLAKQSDAVLLGAMGGPKWDGLDYSIRPERALLALRQELGLF
                     ANLRPVKLFSALASASTLKREVVEGTDMLVVRELTGGIYFGQPKGVTMLPDGTERGVN
                     TEVYTTPEIERIAHVAFQAALKRRKKVTSVDKANVLEVTELWRKVVTRLHKKEEGYAG
                     IELEHILVDNCAMQLIRNPRQFDVVVTTNMFGDILSDEAAMLTGSIGMLPSASLGGKV
                     GMYEPVHGSAPDITGQDKANPLATILSVAMMLRHSLDQGSAADRIEKAVEVALDEGYR
                     TADIQEPGCKLLGCRPMGQLVRDKIAAAKG"
     gene            17769..18791
                     /locus_tag="VMZ02_01440"
     CDS             17769..18791
                     /locus_tag="VMZ02_01440"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013923992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HUR70617.1"
                     /translation="MKKEMYNVAIVGATGAVGEQMREVLDERQFPVGELRLLASERSV
                     GQFLPFQNRQLPVEVLKADSFNGIDIGLFSAGGSVSTEFAPMAVAAGAVVVDNTSVFR
                     MEPDIPLVVPEVNAKEIANYKTRGIVANPNCSTIQMVVALKPIHDAARIKRVVVSTYQ
                     SVSGAGRKAMEELSQQVAALFNGKEIEKVKFPHQIAFNCIPHIDLFMDGGYTKEEWKM
                     IEETRKILGEPNLPITATTVRVPVFCSHSEAVNVETYVKLSANEARRILREAPGVIVA
                     DEPENNVYPMAIDAAGKDATFVGRIREDKSIANGLNLWVVSDNLRKGAALNAVQIAEI
                     LIRDYL"
BASE COUNT         4456 a         4710 c         5458 g         4399 t
ORIGIN      
        1 aaagagattc ttttctgcgt ttagtggttc gtctcacgga caggacttgt tcgtgcattc
       61 gcgctaggtg ccagcgtaaa tttgtcgcca gtaatccaaa ctggccgggg cgcagtcggc
      121 gttaaatatc ccagcaatga ccggtttaga ccccagtttc atattttgaa gacagctttg
      181 agtttcgaaa ggagaagcat agtgtatggt tcgtggtaga actcgatccg ccgagccgga
      241 aactgtagag gaagtggtta aggaagtcgt caaggacaac gggttaaatc tcaaagccct
      301 caaggaaaag aagatcggcg atctcgcgct gatcggcaaa acttttaata ttgagggcgc
      361 cgccaacatg cgcaaacagg atttaatctt tgcgatcctg caggcgcaga ccgagcaaaa
      421 cggtcacgtg tatggcgagg gtgtcttgga gactctgccc gatggttttg gctttctgcg
      481 tgcccccgat tacaactacc tgcctggtcc ggacgatatt tatgtttcgc ccagtcaaat
      541 tcgtcgcttc agcctgcgca cgggagatat catctccggt catattcgcc cccccaagga
      601 aggcgagcgt tactttgcct tgctcaaggt cgaaactatc aactacgaag atcccgagcg
      661 cgcccgagaa aagatcctat ttgacaatct aacgccactt tatcccaacg aacgtttgtg
      721 tttggaaaat cagccgaccg actacactac tcgaattgtc gatttgctga ctcccatcgg
      781 caaaggtcag cgtggcttaa ttgtcgcagc accgcgcacc ggtaaaacga tgatgttgca
      841 gcatatcgcc aaggcgattg cgcataatca tcaagaagtg gtgttaattg ttttgctaat
      901 cgacgaacgg ccggaagaag tcaccgacat gcagcgttca gtcgatggcg aagtcattag
      961 ttcaacattc gacgagccgg cgacacgtca cgttcaagtg gcggaaatgg taatcgagaa
     1021 agccaaacgg ctagtggaac acggtaaaga cgttgtgatc ttgctggata gcatcacgcg
     1081 tttggcgcga gcctataaca ccgtggtgcc gccgagcggt aaaattcttt ccggcggcgt
     1141 cgattccaac gccttacaca aacctaaaaa attcttcggc gcagcccgaa acatcgagga
     1201 cggtggcagc ttgacaatta tcgccacggc tttgatcgat actggcagcc ggatggacga
     1261 agttatcttc gaagagttta aaggcaccgg taatatggag gtgcatttgg accggcgctt
     1321 aatggataag cgggttttcc ccgccatcga catcaataaa tcgggcactc gtaaagaaga
     1381 gctgttaata ccgcgcgagg atctcaatcg catttggatt ttgcgcaaag tattatctca
     1441 attgagtgtt gtcgaatcga tggagttttt gctcgataag atgcatggca gcaagaccaa
     1501 caaggaattt ctcgaatcga tgaaccagta gttccgccgc gattgtaaac ttgcgccaac
     1561 tgacaatctc tgttaaaacg ccgagcattg tcgtcgttcg tgtcgtcctc gggtcgatga
     1621 gattctgcct gcccggcagc cctcgcggcc gatagtttta ataattcaat acacaccctc
     1681 gccgattgtt ggcgcggtgc ctacttttag gtgcgctggc gaaaagacgc gaggggagga
     1741 aaaaccgaag gagagtatta tggcagagat ttcaatgaaa caattactgg aggctggcgt
     1801 ccatttcggg catcagacca gccggtggaa tcccaagatg aagccctaca tctttggcgc
     1861 ccgcaacggt atacacatta ttgacttgca gcagacggta tcgatgttca aagaacttga
     1921 gggtgtcgtg cgggatctgg cagcatccgg cggccatgtt cttttcgttg gaaccaaaaa
     1981 acaagcccag gacgcgattc gggaagaggc tacacgctgt ggtatgtact acgtccacaa
     2041 ccgctggctc ggcggtaccc tgaccaattt tacgacgatt cgccaaagta tcgagcggct
     2101 acgtaagatc gaggaaatgg aagccgaccc gaaaattgtc gaggccttaa ccaagaaaga
     2161 gatgctcggg ctgcaccgtg aaaaagataa acttgagcaa tcgctcggtg gtatcaaggc
     2221 gatgcgcaaa ttgcccgacg ctatattcgt gatagatccc aaacaagaag aaatcgcggt
     2281 caaagaagcg cgcaaactgg gtgttccggt gatcgcggtt atcgacagca attgcgatcc
     2341 agatatgatc gattataagg tacccggcaa cgacgacgcg attcgcgcga tccggttgtt
     2401 ctgtcaggct atcgcggaag cggtaatcga aggccgaacg atatatgagc agtcgttggt
     2461 caaggacaag gccgatgacc gacctgcgga agcggtattt gcagcagaaa gcgcagccgg
     2521 aggagtttag gcgagatgga agtgacagcg agcttagtga aagaactccg ggacaagacc
     2581 ggagcgggca tgatggactg taagaaggcc cttgccgaga ccggcgggga cctccagaaa
     2641 gcaattgatt acttgcgcca gaaagggttg gccgcggcag ctaaaaaagc cgatcgtgtg
     2701 gcatccgatg gtgccgttgg cgcgtatgtg catgcgggcg gaaagatcgg tgtcttagtc
     2761 gaaattaatt gtgagactga ttttgtcgct cgtaccgctg agttccagaa tcttctcaaa
     2821 gacattgcca tgcaagtcgc ggccgcgaat ccgcgctgtg tgcgccgcga agaagtcact
     2881 gccgaggaat ttgagcgcga aaaaataatt taccgtcaac aagcattgga ttccggcaag
     2941 ccggagaaag ttgtcgagat gatcgttcag ggcaaaatgg agaagttcta ctccgaagtt
     3001 tgcttgatgg agcaagcgtt catcaaagat cctgacaaaa aagtcggcga tatcgtcaat
     3061 gaggccatcg cacggttggg cgagaacatt caggtgcgac gctttgctcg ttatcatctc
     3121 ggtgaaggcg gggcgaaaga ctaaatcgca gcgaaagccc ggcatgaata acggtggtcc
     3181 gaaatataaa cgaattattt taaaagtaac cggggaagtg tttgccggca ccgggaattt
     3241 cggaatcgac ggcgcgaccg tcaaagcttt tgcgcaggag atcaaagagg ttaaggatct
     3301 tggctgtgag ctcgcgctag tgatcggcgg cggcaacatt ttccgcggcg cagtagccag
     3361 tgagatcggg atggatcgcg ccagcgccga taccatgggt atgctggcga ccgtcattaa
     3421 tagtttggcg ctgcaagatg ccctcgagaa gatcggggtt tcgacgcgag tcttatcggc
     3481 catcgagatg cgccaagtcg ccgaacccta cattcgcagg cgtgccaccc ggcacttgga
     3541 aaaaggccgc gtcgttattt tcgccggtgg caccggaaat ccatatttca ccaccgacac
     3601 cactgccagt ttgcgcgcca tggaagtgag cgccgaggtg attctcaagg cgaccaaggt
     3661 ggatggtatt tatgatgccg acccacttaa gcgatccgac gccaacaaat tcgaggaatt
     3721 gacttatatc gaagttctca accgcgaact caaggtgatg gactcgacgg cgatctctct
     3781 gtgcatggac aatcagctgc cgatcattgt tttcaacatc atggaaaagg gcaatatcaa
     3841 gcgcgtcgtt tcgggcgaat ctattggcac tctggtgagt ggaggtaaac gatgaatgaa
     3901 gcaatcttta cccaggtgca aaaagagatg gacggctcgg tcgcagtgtt gcgcaaagat
     3961 ttgacgaaac tgcgcaccgg tcgtgcgtcg acgtcattgt tggagcatat caccgtcgac
     4021 tactatggcg cgtcgacgcc gttaaaccag ctcgcgacgt tgagcgcccc ggaaccgcgt
     4081 ttgttggtga ttcagccgtt cgaccgtgcg gcgatgaccg agatcgagaa agcgatttta
     4141 aaatccgatc ttggtctcac tcccaacaat gacggcaaaa tcattcgcat ccccattcct
     4201 gcgttaacag aagagcgccg caaggaactc gtcaagcatg tgaagaagat cgccgaagag
     4261 tatcgcgtct ccgtgcgcaa tcatcgccgg gttgccgttg agcaaatcaa agaagcggaa
     4321 aagaaaaaag agctcaccgc agacgagacc aagcatggtc tcgatcgggt gcaaaaaatc
     4381 accgacgact tcatcggccg aatcgacaag atcgtcaagg ctaaagaaga cgaagtcatg
     4441 gcgatctgac agtaacgttc tagttgcgca gcttaccggg tattagccgg tgtaaccctt
     4501 ccaagcaaga cctcctcatt caccgatgaa cgacacttta cgcaggcttc cgtggaccta
     4561 ggcggcttag ataagagccg tttgccgcag catgttgcta ttattatgga cggtaacgga
     4621 cgatgggcga cccagcggcg caagcatcgc atcgaaggcc atcggcgcgg gaagacctct
     4681 gttcgggtca tcgttgagat gagccgaagg atcggcatcc gttacctctc tctctacgcc
     4741 ttttccaccg aaaactggct tcggcccgaa gacgaggtcg aagccctgat gggattgctg
     4801 gaacattacc tgatcgcaga gcagactaag atgatgcgct acggcattcg cctgctggcg
     4861 gtgggtaacc gtaaccggct gcccgcccag cttcgacgaa cattggagag cgtgatcgag
     4921 gcgaccgcca aaaaccaacg tatgacggtc attctggctc tcagttacag tggtcgcgac
     4981 gacatcgtgc gcatggcaag ggttttggcg cagcaggtca gcgccggcaa ttgccaagcg
     5041 gattgcgtcg acgagcagtg gatttcgtct catcttgata cggcatcggt accggacccc
     5101 gatctgttga ttcgcaccag cggtgagatg cggattagta acttctttct ttggcaaatc
     5161 gcttacagtg aactttacgt cacgccgacc ctctggccgg attttcgcga aaaagaatat
     5221 ctacaagcac tcttggattt tcaacgcagg cgccgacgct tcggccgcac cgacgaacag
     5281 atggccgaaa aacctttggt catcatggag gcgggtggac ggaaatctta aagcgcggat
     5341 cggcaccgcg gcagttgggc tgccgcttct ggtgtggttg gttgggtgga gtgcgcagtg
     5401 gttattttca ttagtgttct ttttgctcac ggtggcggct ttgcgagaat actttgcgat
     5461 gatttgcccc ggcgagtttt ggcagcaggt ttggggaatt gttttcggca gtgccattgg
     5521 attgacggtt ttggctagtc ccgcatccgt gatggcacta tggctcagcg tctttttgat
     5581 tattctattt gccagcactc tgtgctcagg gggagctctc attgatcggc tcaaccgctt
     5641 gggttggacg ttgctgggcg gactttatat cggattttta gtgccgcagc tcgcgctgct
     5701 attcgaattt agagacggcc ggcgctgggt cgcatttgta ttcctggtca tcatggctgg
     5761 cgacacttca gcctatttta tcggccggcg attcggtcgg cgaaaacttg caccggaact
     5821 cagtcctggc aaaaccgtgg aaggttccat cgcctatgtg attggcagca tactgattgg
     5881 catgctcgca ggggtgctgc tggcgctgcc tgtagcgctg cttgaggtcg ccctggtcgc
     5941 attagtcatg agcctgcttg gccaaattgg cgatcttttc gagtcgttaa tcaaacgcgc
     6001 cttcgcagtt aaggattcgg gagagttatt accggggcac ggcggagttt tggatcgtct
     6061 cgacagtttg atttttccgg tggtcttcgc aacgttctat ctgacagtta ttcatccatg
     6121 agaaccattg cccttttggg atcaaccggg tcgatcggtg ttagtaccct gtcattagca
     6181 agagaattcc cggaacgctt taaaattcac ggcatggtag cagggcgcaa tctgcagttg
     6241 ctggccagcc aaataaaaga attctcaccg gcgctggtcg ccatcgaacg tgaggatgat
     6301 atcgctcagc tgcgtaaatt tttgggcaag cacaaagtgg atattttgtc ggggcaggcc
     6361 ggcgctattg aggttgccac agcctctaaa gtcgacgtcg tgctggcggc gatcgtcgga
     6421 ggcgcgggct tgatgccgac cctgcggggt ttgctagcgg gaaaagaaat cgctctggcg
     6481 aacaaagaag cccttgtgat ggctggcgaa ctttttatca aagccgccaa gaaaaaaggt
     6541 gttcgactct tgccggtgga tagcgaacac agcgccattt ttcagtgtct gcaaggcaac
     6601 cagcgcggcg acgtcgataa aattattttg acagcctcgg gcgggccgtt cctgcgcacg
     6661 ccgcttcagc gattagcaaa tgtcacaatc gatcaagcac tcaaacatcc caattggaaa
     6721 atggggcgta agatcaccat tgactcggcg actatgatga acaagggact ggaagtcgtc
     6781 gaggcgcgct gggaattcga tatggaaccg agtggtatcc aagtcgttat tcatccgcaa
     6841 agcatcgtgc attcgatggt gcgttatcaa gacggcgcga tcatggcgca gctgggaatt
     6901 gccgacatgc gcattccgat cgcctacgca ctagcctacc cgcatcgcct gaaaggcaac
     6961 tgggaacctc tagacttgac caagcataaa gggctgaact tcctccccgt agaaaagaag
     7021 cgctttcccg gtctgtcgtt ggcctactcg gccatcgagg agggcggtac tatgccggcg
     7081 gtgctcaacg cggcgaacga ggtggccgtg gcggcattct tggccggcag gataggattc
     7141 cgcgaaattc atcgtataat tgacaagaca atgcagcgcc atcagagcaa gcatgcaaaa
     7201 aatatagacg aagtcattga agtggaccgt tgggctcgcg aggttgcaac aaccgaagtt
     7261 cattagttca aaatcgagga gtatcgcgat ggaaggtttt ctttattcga tcggttcagc
     7321 cgtcataggt ttggggctac tgatcgtgtt tcatgaattc ggccatttct tattggctaa
     7381 attatcagga gttggggtgc tgactttttc cgtgggcttt ggcccgaaaa ttcttgttcg
     7441 caagaagggc gaaaccgagt atgccctgag cgcgtttccc ctcggcggct acgtaaaaat
     7501 gatcggtgag gatccaaacg aggaggtgtc ggcagctgac agagaacggt ccttcgccca
     7561 taaaagtctg ctcaagcggt ttgccattgt ctttgctggt cccgccttta acctcatttt
     7621 ggcagtgatt ctgctgatgc tggtgtttgt tttttacggc gtgccggtca tgtcgacgct
     7681 cgttagcggc gtggaaaaag gctcgccggc agagcgggcg ggcattacca aaggcgatcg
     7741 catcgttgct attgacggta aaccggtggt cgagtgggag aatctgtcgc agacgattaa
     7801 atcgagcgaa ggcagaccat tgaatttgga aattcgccgt ggcagcgagc ttgtcaagct
     7861 aacggtccaa ccgacgcgta aggaaggacg caatattttc ggcgaacgca aagacgactg
     7921 gctgatcggg atcggcagtc aggtttcgat agagaagggt aagccgggct tggcagttgg
     7981 cagagcattt tctcaaacat gggattacat caagatcacc gtacttgggt tctacaagat
     8041 gattatgggc gatgtctcgc cgaagaatat tggcggccca attttgattg cgcagatggc
     8101 cggccagcag gctcaggaag gggtcggcag ttttctcgcc tttctcgctg tgcttagcat
     8161 taatctcggc gtgctcaatc tcttgccagt gccggtgtta gacggcgggc atctgttatt
     8221 ttttctcgtt gaggcggtga ttcgcaagcc agtgtcgata cgcgtgcgcg agatggccca
     8281 gcaggtcggt atctgtctct tgggcctttt aatggtttat gcgttctaca acgatatcgt
     8341 gcgattcttc gaaaaacagg tcggtggatg aggatcctgg gcatagatac ggctacagcc
     8401 gtctctagtg tggctttttt cgaagatgga caactcactg ccgaggctac ttttccgccg
     8461 aggaatcgaa ctggagaagt cctaagcgtc aagagcagtc actcggaaac tatcttgccg
     8521 ttgataagct cagttcttga caacgcgcat cgcaagattt ccgatattga tgtcattgca
     8581 gtttccatcg gtccaggctc gtttaccggt ttacgcattg gtctggcttt agtcaaagga
     8641 attgcttacg aatgcagtgt gcccgtagtc ggtgtgtcga gcctcgaagc ccaggcggct
     8701 cggttcacta aaatatccgg tacaatctgt tcgttgctcg atgcgcgtaa gcacgaagtt
     8761 tatgccgctc tgtttgaaca acgtgaggga agtctgctgc gttgttcggc cgaccaggcg
     8821 actgaggtca cggatctgat ggctttgttc agctcaagtt tgcgcacgag cgtcataaag
     8881 tttgtcggca acggtgcgga actttatcag gagcagatac tgcatttatt cggcagtcga
     8941 gcgcagatca ttggcgaggc ttgcggctgc agtgccgctg gaggtgtggc tcgggtagca
     9001 tcaacgcgtt cgccagcgat ttctaggact gatttggggc ctttggtgcc gctctatttg
     9061 ggcacctcgc aggcgctcgg tattagtcgc aagattcttt ctaactcgtt gaaatagtga
     9121 agagaaagcg cgttgacaaa gtaccgaccg taagttagat tgagcccgct catagtttag
     9181 aatcactaaa aggaggccgc acttatggag cacagtgaag aattggcgat cgtctccctc
     9241 ttggataagg atccagaact caaaaggtac tacgatgagc accaagcctt tgagaaacag
     9301 cttgccgcgc tgcatcacaa agggcatttg tcagtagacg aagaactcga aataaaacga
     9361 attcagaaac taaaactggc tggcaaagat aagatgatgg agcttttggg aaagcaccgt
     9421 cagttccgtg atcgcagcag ttaaggagtc gtaatgaaga ttaccggagc ggaaatattt
     9481 tgcgaatctc tacgccgcga aggtgtgaaa actattttcg gcttgcctgg cggggttgtg
     9541 ctgaaaattt ttgacgtact ttgccagcga aaagatttgc aattgatatt gacgcgccac
     9601 gagcagggcg ctgctcatat ggccgagggc tacgccaaag caactggtaa ggctggagtt
     9661 gtgctggtga cctcaggtcc tggtatgacc aacatcgtta ccggtttggc cgatgctcac
     9721 atggattcag tcccattggt ggcgtttacc gggcaagtct cgacggatct aatcggcaat
     9781 gatgcgtttc aggaagccga taacgtcggc atcagccgac cgtgtaccaa gcacaacgta
     9841 ttggtgaaag atgtcgccga cctcgcccaa gccgtgaagg aagcgttcta catcgctacc
     9901 agtggacgac ccggccctgt tttggtcgat attcccaaag atgtcagcat cgcaaagaca
     9961 gaattcaaat atcccgacaa ggtaaatttg cgcggttaca acccgttcac cggcggtgac
    10021 acatcgcaga ttaagcatgc ggctgaagcg attatgacgt ccaagcggcc gattatttac
    10081 gttggcggcg gcgcagtttt tggggatgcc gccgatgagg tacgcgaact tgccgagatc
    10141 acccagatac ccgtgacgat gaccttgatg ggtctgggga ctttcccagg tacccacgag
    10201 ctgtccttgg ggatgttggg tatgcacggt ggctactgga gcaatatggc gatgcatgag
    10261 tcggatctgc tgatcgccgt aggcgctcgt ttcgatgatc gtgtcaccgg caaactttca
    10321 gaattttgtc caggcgctcg ggtcatccat atcgatattg atccgacctc tatccgaaag
    10381 acttttcacg cgcatatccc aatcgtcggc gacgtcaaat cggtgctgcg ccaaatgaac
    10441 gtcacgttgc gttcattgga cggtaatcca acggcgatga aagcgcgacg tgcgccatgg
    10501 ctcaagcaaa tcaatgcttg gaagaaggag cacccgctct cttacaaaca ggacgacaaa
    10561 ataaccaagc ctcagtacgt cgttgaaaaa ctatacgagc tgactaagca tgaggcgatc
    10621 gtggccaccg atgtgggaca gcatcaaatg ttcgcggcac aatatttcaa aggcaagaag
    10681 ccgcgcactt ggcttacctc cggggggcta gggaccatgg gttttgggtt cccggcggcc
    10741 atcggcgctc aagtggcgta tcccgaccgc caggtggttt gcatcaccag tgaaggtagt
    10801 ttccagatga atctgcagga attggtggtc gccgctgaac ataagttgcc ggtgaaaatc
    10861 gtcctgctca acaacggcgt gcacggcatg gtgcggcaat ggcaagatct gttctacgaa
    10921 agccgctatt cggcgagcat cctcggcaaa atgccagact ttgtaaaact cgcggacgcc
    10981 tatggcataa aaggtttacg cgcagtaaag cccagcgaag tggatggagt tctcaaagag
    11041 gggctaaagc ataaagggcc ggtgctcatg gacatccaca ccgatccgtt cgaaaactgc
    11101 tatcccatga ttcctgccgg cggagcgcaa catgaaatga tgttggaaga cccgcctgaa
    11161 ctcaagttaa ctcgtaaggg cggagtacat aagagaaagg tggatgaagg cgacggggtt
    11221 cttcctgcct gatattttta ggaatggaac atatcatatc ggttcttgtt gaaaataaat
    11281 ttggtgtctt agctagggtt gccggtcttt tcagtgcgcg gggagataac atcgagagtt
    11341 tgtcggtggc gccaacactc gaccccacga cttcgatgat tacgattgtt acggtaggcg
    11401 acgatcgcat tatcgaacag atcatgaaac agctcaacaa agttgtcgaa gtgctcagcg
    11461 tgatcgacca tacagaaact gacttcttgg atcgagaaac ggtactgatc aaggttaaca
    11521 ccaagccgga gcatcgggac gaggcgattc gtattgccga aatttttcgt gccaagatcg
    11581 tggactcgtc gccgacgact tacaccatag aaatcaccgg cgacgtcgat aaagtcgagg
    11641 cgatgattgg catgctcaag ccgcttggga tcaaggatct gatacgtacg gggcgcattg
    11701 cgatctctcg cgaaaccacc agaacagcgt cgccacgccg ggaaccggcc ttgctgaagc
    11761 cctaatcgat agcgtcatcc ttcggcaact tcgcaggata aaaacgccgg ttcatgaatg
    11821 atcatgaccg gcgtttttgt tcgggacttg cgggccggga gtaatttact tcgtatcttt
    11881 gattttgtcg gctgcctctt tgccggcttc tttagttttg ccgatcacgt ctttgccaaa
    11941 ttctttggcc ttctcaccaa cgtctttcat ggcatcgcca gctttttcca tggctttacc
    12001 ggccttttcg gtcgcttgat cgattgcctg accgacggca gcacccgcct tttcagctgg
    12061 gcctggattt tgtttctcga cattgtcgca agctgccgat gtaaccaaca acgctgtggc
    12121 aatcgctcct aaaacgagct gcttgacgta tttcatcggg tctcggatcc cttccagctt
    12181 acatatcgag tatgttttta gtcttaccat ggcgttactg agcggttcaa tggatttatc
    12241 gatgcgtagc gggtgcacaa aaaaattcgt caatagttgt tttcgctaga aaagattacc
    12301 atcccatcga atggtaatac gcgatttccc atgagtctgc gcggttgttt gcagtggttc
    12361 tagaaagtaa ttcctgtttg gttccacata cttgtcgttg acttagcata ggtcaatcgg
    12421 catagtaact agtgaaaagt tcataggaga atctaattca tgcaagttta ctacgaccgc
    12481 gatgcggatt tgaatgtttt gaagagcaag aaagttggcg tgcttggtta cggcagtcag
    12541 ggacatgcgc atgctaacaa cttgcgcgac tctggcgtcg atgttgtggt tggtctgcgg
    12601 cgcggaagca gttcttgggc gaaggcggaa aacgccggcc ttaaagtggc tgaaaccgcc
    12661 gaagcggcag ccatcgccga tatagttatg gtcctgctcc cggatgaact ccagggggat
    12721 gcctacgaga gtgaaatcag accagggctt aagaacggca actacctggc gttcggccac
    12781 gggttcaata ttcatttcaa gagaatcgtc gcacccgccg gagtcaacgt gtttatggtt
    12841 gctcccaaag ggccgggcca tctagtgcgc agcgaatacg agaagggtcg cggcgtgcct
    12901 tgtttgttgg cggtgcacca ggatcccacc ggcgacacca caaaaatcgg cctcgcctat
    12961 gccagcgcca ttggaggggc acgagcggcg gtgattcaaa ccactttcaa agacgaaaca
    13021 gaaaccgatc tattcggcga acaatctgtc ttgtgtggcg gtctctgtgc cttgatccaa
    13081 gccggttacg agaccctcgt tgatgccggt tatccacccg agatggctta tttcgagtgc
    13141 gtccacgaag tcaaactgat tgtcgacctg atttacgagg gcggcatggc gaatatgcgc
    13201 tactcgatca gcaatactgc ggagtatggt gatttaaccc gcggcaagcg agtcatcggt
    13261 ccggaagtgc gtcaggcgat gcagaaaatt ctcaaagata ttcagtccgg ccagtttgcc
    13321 gaggaatgga tcaacgaata caaatcggga gcgccgcact ttaccgagct tagaaaagag
    13381 caggccaatc atccgctgga aaaagtcggc gccggtttgc gttcgatgat gccttggctg
    13441 ggccataatc gattggtcga tagaagcaag aactaggcga tttccactgg attgtagaat
    13501 cgctcctgcc gggtacgatg cgtattgcga aagaaggttg gccattgatt ctgccggccg
    13561 cggctgtagc gctcatggcg ttgctgcttg gttgggtggt ggtcggtgtc gctctggctg
    13621 tgttgaccat cgccatcgcg gcgttttttc gcgatccgga gcggaaaatt ccaactggtg
    13681 acgggttggt ggttgcgccc gccgatggcc gaatcgtcgc gatcgcaccg gttgagggga
    13741 atgctttgtt cgccgacgcg gtgacgcgta tcagcatctt tttatcgccg ctggacgtgc
    13801 atataaatcg tgcgccggtg gccggtcgcg tcagtgctgt gcgctatcag gctggcaagt
    13861 tcattgccgc gtttaaggaa gaggcgacgc gctacaacga gcaaaacgct ctccacatgg
    13921 tcgacgagcg cgagcgtaac ttgggggtag tccaggtcgc cggggtggtg gcccgccgca
    13981 tcgtctgccg tgccaaagcc ggcgatattt tgtcgcgcgg cgaacgtttc ggtttgatca
    14041 tgttcggttc gcgtaccgac atttatctgc cccaaagttg taggttagaa gcgactgagg
    14101 gtcagagggt cacaggcggt gagaccatca ttgggaggtt cccatgagcg cgatgaatgc
    14161 tccggagccc gagcggccgc aagagaaaga taaattacgc ctattgcgcc gtcgccgtga
    14221 gcggccgcgt atgaccttac gtcagagaat gccggcggag aagctgcgca aaggcgtgta
    14281 tcttatcccg agcctgttta ccgccggcaa tctgatgtgc ggatttttct ccatcatcgc
    14341 gacgtttagg ggtgaatacg tcaacgccgc tttgctaatt ttgcttgcca acgtgttcga
    14401 tggagtcgac ggttatgtcg cccgcctcac ccggacgacc agccaatttg gcgtcgagtt
    14461 cgattccctc gccgatgttg tcgcgttcgg cgtggcgccg gcagtcttgg tctatatctg
    14521 ggcgctggtg ccgtggcaaa actggggttg gctggcagcc gcgacttacg tggtctgcgg
    14581 cgccgcgcgg ctgtcgcgtt tcaatgtcca ggcgcagggc cctgcgaaaa attactttgt
    14641 cggtttgccg atcccagcgg cggcgcagat gatcacgtca tcggtcatct tgtactatta
    14701 tctcggtttt gagggtgcgc cgacgcgcca tttaacgttt ttgctgttaa tttatggctt
    14761 ggcgattcta atggtaagca gcattccgta ttttagtttg aaaaataacg atatgaggaa
    14821 gcgccatccg gagtggatgt ttgtttccgg cgtgattttg attacactca taatcgccga
    14881 gcggcatatc atgtttttta caattgttct cctgtatacg ttatccggcc cctggttatg
    14941 gtgcttaact aacctcaagc accggcggga gttgagacgg gaggcggcga aagttttgtc
    15001 gtaagttcct ggctcaaata aaagtgaaac aactcccgtt ggccatgccg cgggagtttt
    15061 catttcgggt agggccgacg gataacgata acgggagaca tcaaaaatgg atcacatggc
    15121 caaagataat gtagtgcaga ttttcgatac gaccttgcgc gatggcgagc agtcgccggg
    15181 cgcgagcatg accgtggaac aaaaactggt gatcgcacgg caactggaga agctcggcgt
    15241 cgatgtcatc gaagcgggct ttgccgcctc gtcggacggt gacttcgaat ccgtgcggcg
    15301 cgtggctttg gaggtaagcg aaccgcgggt cgtgagtctt gcgcgcgccc aggagagcga
    15361 tatcagtcgc gcgttgaaag ccgtcgaagg cgccaagaag cctggaattc acacttttat
    15421 cgcgacctcc gacattcacc tcaagcacaa gctgcgcatg acgcgcgaac aggtcgtcga
    15481 agccgcggtc aaagcagtga cctatgccaa gcagtttacc gactatgtcg aattctcagc
    15541 cgaagatgct tcgcgcagtg atccggaatt tttgctgcag atcttccgcg aagtgatccg
    15601 cgccggcgcg aagacactga atgttccgga caccaccggt tatgcgatac cgtcggaatt
    15661 tggcgctttg atcgccaatc tgatcgaacg gacggccggc ggcgatcggg tcatctggag
    15721 cgcccattgc cataacgatc ttggtctggc agtggccaat tcactggcgg cggtgcataa
    15781 cggcgcgcgt caaatcgagt gtacaatcaa tggcatcggc gagcgcgccg gtaatacttc
    15841 tctcgaagaa gtggtcatgg cgctgcgcac acgcaaaaat tatctcgatc tcgacactaa
    15901 tattcatagc gagcagctct atcccacctc gcgcttgcta tcgcaggtcg tcggtatccc
    15961 gatcccgatc aacaaaccga tcgtcggcga taatgctttc gcccacgagg cgggcatcca
    16021 tcaggatggc gtgctcaagc acaagcagac atatgagatc atgacgccgg agtccatcgg
    16081 tattcccggt aatcgtctcg tcatgggcaa gcattccggt cgccatgcct tcgatgaacg
    16141 attgaagcac ctgggtttca atctgagtaa agaagacatg aaccgcgcct tcgttcgttt
    16201 taaggaactc gccgataaaa aaaagaatgt ctacgatgag gatatcgaag cgattgtcgc
    16261 cgaagaagtt ttgcgcatgc cgggacgtcc ggataaattc gagtttctct acatgagcgt
    16321 caactcgagc agcgacggcg tgccaaccgc gacgatcaaa atgcgcgtcg atggcgcgga
    16381 aaaaatggac catgcctcgg gcgacggcgt ggtcgatgct tgctacaaag tgattaccaa
    16441 gatcactgac tcgcagtcgc aactggtgcg ttattcggtc aacgcgatta ccggagggac
    16501 ggatgcccaa ggcgaagtcg catgcttgat agacgacggg ggtatccgcg tgtcgggtca
    16561 gggttcgcac accgatatta tcatggcgag tgcattggcg tatattaacg cgctcaataa
    16621 attggaagaa cgaaagcgct atcggcaggt ggtggagaaa gagggaccat gaattacaac
    16681 atcgcggttt tccctggtga cggcatcggc ccggaggtta tgagcgaggg tacagcagtt
    16741 ctcaaacagg tcgccgagct ttacggcttc acggtaaatc ttgagtcagg gatggttggc
    16801 ggcgcttcga tcgatgctca tggcaagccg ctcaccgacg ccgttttaag tctcgccaag
    16861 cagagtgatg cggtcttgct cggcgccatg ggcggaccca aatgggacgg cttggactac
    16921 tccattcggc ccgagcgagc gcttctggcg ttgcgtcagg agttgggttt gttcgccaac
    16981 ttgcgtccgg ttaaattgtt ctccgcgctg gcatcggcat cgacgctaaa aagagaagtt
    17041 gtcgagggca cggatatgtt ggtggtccgc gagctcaccg gcggtattta tttcggccag
    17101 ccgaaaggcg tgacgatgct tcccgatggc accgagcgtg gcgtgaatac cgaggtctat
    17161 acgacgccgg agattgagcg catcgcccac gtggcgtttc aggccgcgct gaagcggcgc
    17221 aagaaagtga cctccgtcga taaagccaac gtgctggaag tcaccgaact gtggcgcaag
    17281 gtggtgacgc gactacataa gaaagaggaa ggctacgcgg gtatcgagct ggagcatatt
    17341 ctggtcgata actgtgccat gcaattgatc cgtaatccgc ggcaattcga tgtcgtggtg
    17401 acgactaata tgttcggcga tattttaagc gacgaagcgg cgatgctaac cggttctatc
    17461 ggtatgctgc catcggcaag tcttggcggc aaggtcggca tgtatgagcc ggtccatggc
    17521 agtgctcctg acattaccgg gcaagataag gccaatccac ttgcaaccat tctcagcgtg
    17581 gccatgatgc tgcgacactc cctcgaccag ggctcggcgg ccgatcgcat cgagaaggcc
    17641 gtcgaagttg ccctcgatga aggttatcgc actgcggaca ttcaagagcc tggatgcaaa
    17701 ctattgggct gccggccgat ggggcagttg gtgcgcgata agattgccgc agcgaaggga
    17761 taagtcttgt gaagaaggaa atgtataacg tcgccatcgt cggcgccacc ggcgccgtcg
    17821 gcgagcagat gcgcgaagtt ctagatgagc gtcaatttcc agtcggcgag ttgcgcttgt
    17881 tagcctctga acgatccgtt ggccaatttt tgccgtttca aaatcgacaa ctgccggtcg
    17941 aggtgttgaa agccgattcg tttaacggca tcgacattgg ccttttctcc gccggcggca
    18001 gcgtcagcac ggagttcgcg ccgatggcgg tggcggcagg cgccgtggtg gtcgacaata
    18061 cttcagtatt tcgcatggag ccggatattc ccctggtggt tccggaagtg aacgccaagg
    18121 aaatcgccaa ttataaaacc cgcggcatcg tcgcgaatcc gaattgctcg acaattcaaa
    18181 tggtcgtagc tctcaagccg attcatgacg cggcgcgcat caaacgcgtc gtggtatcca
    18241 cctatcagtc ggtgtccggc gccgggcgca aagcgatgga ggagttgagc cagcaggtgg
    18301 cggcgctgtt caatggtaag gaaatcgaga aagttaagtt cccccatcaa atcgccttta
    18361 actgcattcc gcatatcgat cttttcatgg acggcggtta caccaaggaa gaatggaaga
    18421 tgatcgagga aacccgcaag atcctcggcg aaccgaactt gccgatcacc gcgaccacgg
    18481 tgcgggtgcc ggtgttttgc agccattcgg aagcagtgaa tgtcgagacc tacgtcaagt
    18541 tatcggccaa cgaagcacgg cgaattctcc gcgaagctcc gggcgtaata gtcgcggacg
    18601 aaccggaaaa taatgtctat ccgatggcca tcgacgccgc gggcaaagac gcgacctttg
    18661 tcggccggat tcgcgaggat aagtcgatcg ccaacggtct caacttgtgg gtcgtgtccg
    18721 ataatctgcg caagggtgcc gcgctcaatg ccgtgcaaat tgccgagatt ttaatccgcg
    18781 attacctata gcctcgcagc cacgttcgac ccgccgtagc tgtggctccg gctcaagtct
    18841 cgaaatgtct ctgtcgtgaa cgtcaaactt accgttgaat atgacggcac caattatcat
    18901 ggctggcaag ttcaggcgaa ttgtacctct atccaagcgg tcctagagaa agccgtctcg
    18961 actttcgtcg gcgtacccac tcacgtcaat ggctcgggac gcaccgatgc cggcgtacac
    19021 gcg
//
LOCUS       DATLCH010000052         5387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3893913, whole genome shotgun sequence.
ACCESSION   DATLCH010000052 DATLCH010000000
VERSION     DATLCH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3893913"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VMZ02_01445"
     CDS             <1..1011
                     /locus_tag="VMZ02_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUR70618.1"
                     /translation="SAAAQFVYDLRYRIGEYSLRGFIACLPYVPFTAVERFTYLAAWL
                     SFKFMAQYRQRMEQNVASTLGDEIASEAERKQLIWRAWKNFARGVLDTTAIMHHSKKR
                     IIDGVRLEGEEHLQRALAQGKGVLALSAHLGSFTLIGARLAASGYPFSVVVKHPADKR
                     FARLTDDYRAQIGIHTIPAKPRREAVRGLLKALRENRIVLVIADEFKSGDVMVNFFGM
                     QLPAPRGPATLALRTGAVTLPMFATREPDDTLLLSVGAPISPVPCEDIEASVVATTAV
                     YTNHLEAAIRRYPDQWNWLGLPNRNGKESRAELALRRKEWKKRRAAKGAAGETRKTAT
                     GA"
     gene            complement(1008..1619)
                     /locus_tag="VMZ02_01450"
     CDS             complement(1008..1619)
                     /locus_tag="VMZ02_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUR70619.1"
                     /translation="MQRYEYLKAISGDAQDAPAVSTGWASREWWAVRPSDGNLKTRTL
                     GLVSSIAAGLAIALPHRKVIAIDGDGAFLMNLCGLPTIAQQNPGNLIHLLFDNEIYEA
                     SGGTATASRSTDAVIIAKGTGYKHALWVSSLEEFRLEFRRAWERNELTLIAAKVDPGQ
                     PKDLPPLKLDEIENKYRFMRYLEATENKRILNPGFDSKLGSPN"
     gene            complement(1619..2131)
                     /locus_tag="VMZ02_01455"
     CDS             complement(1619..2131)
                     /locus_tag="VMZ02_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR70620.1"
                     /translation="MTTNDPVFAGQAIVAGLKKAGIDFVATLPDEKMLEVIRAVERDQ
                     ELQHIPLCREEEGIGICAGAYLAGKKTAIIMQNAGFLNSCNALTTTSLQLQIPILMLI
                     YYAGDIGDRGFTTLGSVTEPVLQAMGFRSYVLRKPEEIDDTLAGAQILADDSKKPVAV
                     LLTKSVLGVK"
     gene            2273..2659
                     /gene="gcvH"
                     /locus_tag="VMZ02_01460"
     CDS             2273..2659
                     /gene="gcvH"
                     /locus_tag="VMZ02_01460"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HUR70621.1"
                     /translation="MTEKEVKVSKQHVWVGVEDRHVHLGLTNYIQGALGSVLSVELPD
                     VGDTIEEGEIFAEIESAATVHELVSPVTGRVMAVNPMLEDHPAIINEDPYSEGWLIEV
                     RLKNDDALASLMDMDEYYHFVVKDKS"
     gene            complement(2719..3078)
                     /locus_tag="VMZ02_01465"
     CDS             complement(2719..3078)
                     /locus_tag="VMZ02_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HUR70622.1"
                     /translation="MKFHLSPRWPMYLIGSLIVSLALVTIVGERGVLHLWRLRGEKSR
                     IDEQNYRMQRNNEALRQKIARVRQDDYYLEKLAREELNMVRPGEIVYRFSKSEARRAA
                     SPAESAESSPQPQRSGE"
     gene            3148..4434
                     /gene="eno"
                     /locus_tag="VMZ02_01470"
     CDS             3148..4434
                     /gene="eno"
                     /locus_tag="VMZ02_01470"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HUR70623.1"
                     /translation="MKIQQVFAREVLDSRGQPTVEVEVTLKNRVVGRATVPSGASTGV
                     HEAVELRDGGKRYFGKGVTRAVAHVNRKIAPRLRGKDGRDQIAIDRIMLDLDGSANKG
                     KLGANAILGVSLAVAQAQARSQGLGLYRYLGGTAGKTLPVPMLNVLNGGAHADNNVDV
                     QEFMVVPYGLKSFKEALRAAGEIFATLKKVLHDRGLSTSVGDEGGFAPRVKSNAEAIE
                     LLLEGITKAGYKAGSQVALALDVASSEFYQDGKYVFKKSDGKTRSRDQLVELYQDWVR
                     QYPIISIEDGFAEDDWDGWRIMTGALGKQIQLVGDDLFVTNKVRLKRGIDSGIANSIL
                     VKVNQIGSLTETLETMQLAKENGYTTIMSHRSGETEDVTIADLAVATNAGQIKTGAPC
                     RGERTAKYNQLLRIEEELGKRAVYAGKNAFRVRRTS"
     gene            4394..5191
                     /gene="nth"
                     /locus_tag="VMZ02_01475"
     CDS             4394..5191
                     /gene="nth"
                     /locus_tag="VMZ02_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HUR70624.1"
                     /translation="MPARTPSECAAPAKKRKNAASANAKKRSQRHETLEEKFLRASRI
                     SAYLAALYSEVECPLTHRNNFELLVAVILSAQCTDAAVNQVTPGLFARYPNPNALASA
                     SLSDIETEIRSLGLFRAKAKSLKRCAEQLVEEFNCAVPSSMAQLTRLAGVGRKTANVI
                     RGHAFGEPGIAVDTHCRRLSRRLGLTQHQDPMKIESDLAHILPSAEWTNFSHRLIVHG
                     RRVCFARRPACTHCALNQLCPSATDYLAAGDLNVREISDPSELEGDV"
     gene            complement(5168..>5387)
                     /locus_tag="VMZ02_01480"
     CDS             complement(5168..>5387)
                     /locus_tag="VMZ02_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70625.1"
                     /translation="TADELAIDASKEFVNIANGNACAKLSENGVNLTMLPPEIYETTT
                     TPYPFLTKEVVCVHLASPDSKLDVAFEF"
BASE COUNT         1205 a         1443 c         1548 g         1191 t
ORIGIN      
        1 tcggcggcag cacagtttgt ttacgaccta cgttaccgta tcggcgagta ttcgttgcgc
       61 ggatttatcg catgcttgcc atatgtgccg ttcacggccg tcgagcgatt tacctatctc
      121 gccgcttggc tgtcattcaa attcatggcg caataccgcc agcgcatgga acaaaacgtc
      181 gcctcgacat tgggcgacga aatcgctagt gaggcggagc gaaaacagct aatttggcgc
      241 gcctggaaaa acttcgcaag aggtgtgctc gataccacag cgatcatgca tcattcgaag
      301 aaacgcatca tcgacggtgt gcgtctcgaa ggcgaagagc atttacaacg agccttggcc
      361 caaggaaaag gcgtactggc tttgagcgcc catcttggca gctttactct gatcggcgcg
      421 cgcttggcag cgagcggcta tccgtttagt gtggtcgtga aacatccggc cgataagcgc
      481 tttgcccggc tcaccgatga ttatcgtgcg cagatcggca tccacacgat ccctgccaag
      541 ccgcgccgtg aagcggtccg tggtctgctc aaagcactgc gcgaaaatcg cattgtcctg
      601 gtgatcgcag acgagttcaa gtccggcgac gtcatggtga acttttttgg catgcaactt
      661 ccggcgccgc gcggaccggc gactttggcg ttgcgtaccg gagcggtgac actgccgatg
      721 ttcgccacac gtgagccgga cgatactttg ctgctgtctg tgggcgcgcc gatttctccc
      781 gtcccttgtg aggatatcga agctagtgtg gtggcgacca cggcggtcta taccaaccac
      841 ctggaagcgg caattcgccg ttatcccgat caatggaatt ggttgggctt gcctaatcga
      901 aacggcaaag agtcacgcgc cgaactagcg ctgcgcagga aagaatggaa aaaacgaaga
      961 gccgctaaag gcgcagccgg cgaaaccaga aaaaccgcca caggagctta attaggcgat
     1021 cctaacttcg agtcgaatcc cggattgaga atccttttgt tctccgtagc ttccaggtag
     1081 cgcatgaacc ggtatttgtt ttcgatttca tcgagcttga gcggcggcag atctttaggt
     1141 tgtcccggat cgactttcgc ggcgattaag gttagctcgt tgcgctccca agctcggcga
     1201 aactccaggc gaaattcttc cagggagctg acccacaggg catgtttgta gccggtgccc
     1261 ttggcgataa tcaccgcatc ggtgctgcgc gacgcggtgg cggtgccgcc agacgcttca
     1321 tagatttcgt tatcgaatag tagatgaatc aaattccccg ggttctgttg cgcaatggtt
     1381 ggcaagccgc atagattcat taaaaatgcg ccatcgccat cgatagcgat cactttgcgg
     1441 tgcggcaacg caatcgccaa accagcagcg atcgaagaca ccagaccgag agtgcgcgtc
     1501 ttgagatttc cgtcgctcgg acggacggcc caccactcac gcgaggccca gcccgtggaa
     1561 acggcgggtg cgtcctgggc atccccgctg atcgccttca gatattcata gcgttgcatt
     1621 attttactcc gagtacactt ttggtaagga gaactgccac cggctttttc gagtcgtcgg
     1681 cgagaatctg cgcgcctgcg agggtgtcat cgatctcttc cggcttgcgg agcacgtagc
     1741 tgcgaaatcc catcgcctgc aacaccggtt cggtgaccga tccgagggtg gtgaagccgc
     1801 ggtcgccgat atcgccggca taatagatca gcatcaaaat cgggatttgc agctgcaatg
     1861 aagtggtcgt cagagcgttg cagctattca ggaaaccggc gttctgcatg atgatcgcgg
     1921 tcttcttgcc cgctaaatat gcgccagcgc agataccgat gccttcctcc tcgcggcata
     1981 gcggtatgtg ctgcaactcc tggtcacgtt ccacggcgcg gatcacttcg agcatttttt
     2041 cgtccggcag agtagcgaca aagtcgatgc cggccttttt taaaccggcg acgatggctt
     2101 gccccgcgaa taccggatca ttggtcgtca ttggttttcc tcaagtgaga tggtcggcta
     2161 gctagtttcg gttgactatc gctactgtgg attccatgat gatgtcaaga gagttgccgc
     2221 gacactaggc ttgcagcgga aggacaaagt ttgtagtgat ggaccaccaa ccatgaccga
     2281 aaaagaagtg aaagtcagca aacagcatgt ctgggtcggc gtcgaagatc gccacgtcca
     2341 tctgggtttg accaactata tccaaggtgc tttgggttca gtgctatcgg tggaactacc
     2401 ggacgtgggc gataccatcg aggaaggcga gatcttcgcc gagatcgagt cggccgcgac
     2461 ggtccatgag ctcgtctcgc cggtaaccgg ccgagtaatg gcggtgaatc cgatgttgga
     2521 ggatcatcct gcgattatca acgaagatcc ttatagcgag ggctggctca tcgaagtgcg
     2581 attgaaaaat gacgacgctc tcgcgtcgct catggacatg gatgagtact atcacttcgt
     2641 cgtcaaagat aaatcctgaa acattttgaa atagcgatca caaaggtcgg acgcgatgcg
     2701 gacgctggtg gaagcgtttt actctccgct gcgttgcggt tgcggtgatg attcggcgct
     2761 ctccgccggc gacgcagcgc ggcgcgcttc agacttggaa aaccgataga cgatctcacc
     2821 tggccgaacc atattgagtt cttctcgagc tagcttttcg agatagtagt catcttgtct
     2881 tacgcgagct attttctggc gcagtgcttc gttattgcgc tgcatgcggt agttttgttc
     2941 gtcgatacgg cttttctcgc cgcgcagacg ccacaggtgc agcacgccgc gctcgccgac
     3001 gatggtcacc agcgcgagcg acacaatcag cgaaccgatt agatacatcg gccagcgcgg
     3061 cgataagtga aacttcatca gagttaatgg caatatacca tattttatgt ttttgtcttg
     3121 tctaacgttg aactgcggga gacgacgatg aaaatacagc aagtgtttgc acgagaagtg
     3181 ttagattcgc gagggcagcc gacggtggaa gtcgaggtca cgcttaaaaa ccgcgtggtc
     3241 ggacgcgcga ctgtgccatc tggagcctcc accggtgtgc atgaggctgt ggagcttcgc
     3301 gatggcggca aacgctattt cggcaaaggc gttacgcgcg cggtcgccca cgtcaatcga
     3361 aaaatcgcac cgagactgcg cggcaaagac gggcgcgacc aaatcgccat cgaccggatc
     3421 atgctcgatc tcgacggttc tgcgaacaaa ggcaagctcg gtgccaatgc cattcttggg
     3481 gtttcgttgg cagtcgccca ggcgcaagcg cgttctcagg gattggggct ttaccgttat
     3541 cttggcggca cggcgggtaa aacgcttccc gtgccgatgt tgaacgtcct caatggcggt
     3601 gcccacgccg acaacaatgt cgatgtgcag gaatttatgg tcgtgcccta cggcttgaag
     3661 agtttcaaag aggcgttgcg cgctgccggg gaaatttttg cgacattgaa aaaagtcctt
     3721 catgatcgcg gactatcgac gagtgtgggc gacgaaggtg gattcgcacc gcgcgtcaaa
     3781 agcaacgccg aagccatcga gcttttactc gaaggcataa ccaaagccgg ttacaaagca
     3841 ggttcccaag tcgcactcgc gttggatgtt gcgtccagcg agttttatca ggatgggaag
     3901 tatgtcttca aaaaatccga tggtaaaact cgtagccgag atcaactggt cgagttgtac
     3961 caggattggg tgcgtcagta tccgatcatc tccatcgaag atggattcgc tgaggatgac
     4021 tgggacggtt ggaggataat gacgggtgca ttgggcaaac agattcagct ggtcggcgac
     4081 gatctgttcg tcactaataa agtgcgtctc aagcgcggca tcgattctgg tattgccaac
     4141 tcgattttag tcaaggtcaa tcagatcggc agtttgaccg agactctgga aaccatgcaa
     4201 cttgccaagg aaaacggtta cactacgatc atgtcgcacc gctccggtga gaccgaggac
     4261 gtaaccatcg ccgacctagc ggtcgcgacc aacgccggtc agatcaagac cggcgcgccc
     4321 tgccggggcg agcgcacggc gaagtacaat cagctactgc gcatcgaaga ggaactaggt
     4381 aagcgagcag tctatgccgg caagaacgcc ttccgagtgc gccgcaccag ctaaaaagcg
     4441 taagaacgcc gcctcggcga atgccaaaaa gcgctcgcag cggcatgaga cgctggagga
     4501 aaaatttctt cgggcgtctc gtatatcggc ctatctggcc gcactgtaca gcgaggtcga
     4561 gtgcccgctg acccaccgta ataattttga gctactggtc gcggtaatcc tgtcggcaca
     4621 atgcaccgat gccgctgtca accaggtgac gccgggattg tttgcgcgct atccaaaccc
     4681 aaacgcgctg gcgagtgcat cgttaagtga tattgaaact gagattcgta gcctcgggct
     4741 gtttcgtgcc aaggcaaaat ccctcaagcg ctgtgccgag cagttggtcg aagaattcaa
     4801 ttgcgcggta ccgtcctcga tggcgcagct cactcggctt gccggcgtcg gtcggaaaac
     4861 cgccaatgtt attcgcggcc acgccttcgg cgagccgggt atcgcggtcg acactcattg
     4921 ccggcgctta tcgcgtcgcc tgggtctaac ccaacaccaa gatccgatga agatcgaaag
     4981 cgacttggcg cacatcctgc cgagcgccga atggacaaac ttctcacacc ggctgattgt
     5041 gcacggtcgg cgcgtctgtt tcgcgcgccg acctgcgtgc acgcattgcg ctctgaatca
     5101 gctgtgcccg tctgcaaccg attacttagc cgcaggcgac ttgaatgtcc gcgagatatc
     5161 cgacccctca gaactcgaag gcgacgtcta attttgaatc tggcgacgcg agatggacgc
     5221 agacaacttc cttagttaga aatgggtagg gtgttgttgt ggtttcgtaa atctccggcg
     5281 gcaacatcgt caggttgaca ccgttttccg aaagtttcgc gcaggcgtta ccgttagcaa
     5341 tgttgacaaa ctctttggaa gcgtcgatcg ccaactcatc ggcggtg
//
LOCUS       DATLCH010000053         7833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3068486, whole genome shotgun sequence.
ACCESSION   DATLCH010000053 DATLCH010000000
VERSION     DATLCH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068486"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VMZ02_01485"
     CDS             complement(<1..328)
                     /locus_tag="VMZ02_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70626.1"
                     /translation="MNSRKLVVALFFAPLFSALCMVKMLESMTGLPAILDSDSILQYW
                     WVSMIAGITVFFFIKTADRWGLLVCLAGGIAVVALVDLVDFLGIVQIYRPGVILPSVY
                     SLEIILA"
     gene            complement(423..935)
                     /locus_tag="VMZ02_01490"
     CDS             complement(423..935)
                     /locus_tag="VMZ02_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70627.1"
                     /translation="MQYDPQPTGTPPKPIEPTGFFTGIQFRPILTGAIVDFIASFVLA
                     AFYYGFYVAPNSGAAAAFEEEAAKFWISSDGLAAGLVLGSLGTLMGGFYAGYKAKALE
                     MKHGALVGLTSILVGLAMHSGDSENQFPEWFMALSFAAAMPAGALGGFFAEMFKGAIG
                     ANLRRREPSR"
     gene            1038..1499
                     /locus_tag="VMZ02_01495"
     CDS             1038..1499
                     /locus_tag="VMZ02_01495"
                     /inference="COORDINATES: protein motif:HMM:NF021192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
                     /protein_id="HUR70628.1"
                     /translation="MFNRYDYEAEFYPSLNRLPLDVRRKLDLTGIKISLRDWLAISLE
                     ERLVLCHFPCETDEERKVFIAYLDFLAGKYLARSSAKTAPMDSALWGELNVPDAVVQR
                     SMAMDQVVRASEWRRWPAHHRYALYKSAVSNKQPEAFEQVLLQLRKLAANE"
     gene            1591..2037
                     /locus_tag="VMZ02_01500"
     CDS             1591..2037
                     /locus_tag="VMZ02_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HUR70629.1"
                     /translation="MSSLLIFLAMVAGTALIGIQFGPDAWYQALQKPSWTPPSWLFAP
                     VWGGLYIAIAVAGWLVWRSKALSVTKPILLWLLQLILNGLWSWLFFGLHRPDLAAIDI
                     IALLVTICFFIASAKKISAIAAWLFVPYALWVGFAAALNFSIWQLN"
     gene            2071..3219
                     /locus_tag="VMZ02_01505"
     CDS             2071..3219
                     /locus_tag="VMZ02_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HUR70630.1"
                     /translation="MTDNLFPRFSDREYARRYSTVRAAMSEDNIDALVIFGARGSSEV
                     SYLANYLPQSPCWLLLPRDGDATVFIHFFNHQPCAKAQSIIDDVRWYGPTPMPTLTEE
                     ITKRGLSNSKIGLVSMRSMAYGHVMELQRQFPNAQFVEFGPQFGKIRRVRSEEELVYL
                     RKSGYLTDLACEALENNLRPGLTEQDVLAIVYMAYLKNGGDPGIHFIATTNMDAPDRF
                     VPWQRQSFRVLERGSVVITELTVSYWGYSTQIHRPFAIGKEPTPTYRKLFDAAYDCYE
                     SVRKICKPGTTSEQIVAATCAIEANGFTSYDSVFHGEAGKSPELGTSSAAHPLERWTL
                     EENMVHVIQPNPVTKDFKSGLQLGAAVVVKPHGGEALHNYPFKFPVCG"
     gene            3229..3951
                     /locus_tag="VMZ02_01510"
     CDS             3229..3951
                     /locus_tag="VMZ02_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HUR70631.1"
                     /translation="MNSSQTENIKFLAFDVFGTVVDWRSSVIAEGEQLGKAKGINVDW
                     TAFADTWRSIYRPFMDKVQNGELPWTKLDDLHRMMLAETLEKFAIDGLTVAETEHLNR
                     VWHRLKPWADSVAGLQRLKSRYLVAPLSNGNISLLTNMAKNAGLPWDCVLSAENVHRY
                     KPDPSVYLLAPQLFDLKAEEVMMVAAHEHDLQSARKHGLRTAYVHRPLEHGPGKASEV
                     PPRDRYDIVASDFLDLAKQLSA"
     gene            3967..4962
                     /locus_tag="VMZ02_01515"
     CDS             3967..4962
                     /locus_tag="VMZ02_01515"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70632.1"
                     /translation="MIAPILAVALILTLSFAGISSADAQRIRAASGGLSIIHSLLWVT
                     YEQKLLKKYGLDLEYIAIENGTVGMQALLANEAQFLFSTSSLAVNANLRGSDVTVVAG
                     GLNFIPDKLIVRPEITKPEELAGKRLAISRFGSSSETSAKLTLEKIGVKPESVSLIQL
                     GGVSTRQVALMAGQVQATVLSDPQATAATTAGMKLWVDLSESKWGLPRICFNCFMAKR
                     SFLDANRETASNFLKAVIEGLYLLKKDKPLGVRLIKKYLRLSDEAAAVGYDFYIGKHG
                     EGMLSLPERRGMEFIIAEVTKTNPAASKATPESLRLLEPSFLDEIKKSGLIERVK"
     gene            5131..6465
                     /locus_tag="VMZ02_01520"
     CDS             5131..6465
                     /locus_tag="VMZ02_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HUR70633.1"
                     /translation="MQFDKDALKDLIVFLGLPLLAVIAAFWFAARFVQPAPPDHFVMT
                     TGADGGAYHLFAQRYRDIVKRENITITLKPSAGSIENLQRLQDDKSEFDVGLIQAGVA
                     GGVAPTALRSLGAVSYEPLWVFYRGADNIEKLSQLAGKRIAVGPEGSGTRALALQLLK
                     VSGIDPSAPELVPFGGNEAVKALLDEDIEAAILVASPDAPTVRELANAKDVKLASLIQ
                     AEAFTRRFPFLTALQLPRGAIDLANDLPGRDVTLLATTANLVVKEHFHPALGYLLLQA
                     ASEVHGRAGVLQKSGEFPAPRESEIAIAAEAQRYFKSGQPFLQRYLPFWMANLIERLA
                     VLLLPLVAVVLPLFKILPVLIQWRNKSRVFKWYGELKYLETQVATNADPAPQLDRYFE
                     RLDEIEDGVSHTRIGASYSDYVYNLRLHIELVRNHLHRLEERASETKAHGQM"
     gene            6437..7411
                     /locus_tag="VMZ02_01525"
     CDS             6437..7411
                     /locus_tag="VMZ02_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70634.1"
                     /translation="MKPRLTVRCDRTLGACPFRFGSAIGVILVCLSCTEPTGQIAAGP
                     QREYQQQRQTDENGVEMLLASAPGSRFQLGEQNPMRASNFAIERKTQAASRIDGALQY
                     WNVTAHDLDYTSGGSGKTIRLHIYAGRGQQVHTWKNQKGFLATPSDIRNQEFTAFVRV
                     HNISDPGRAAITLKIRGGAHTDKNPDLASCTMIAFQANSTGAVARFGKELTHPNYDYV
                     TLTPAFDAGLLENRWVGLKLLSYSSPGEPRRVTNRLYLDIDPFEHVGAKPRNNWKQFA
                     KYVDIEGVSTGKYNKLADWGGWQTTLRSDGVSSLDFALISLREVQPGL"
BASE COUNT         1810 a         2234 c         2099 g         1690 t
ORIGIN      
        1 tggcgaggat gatttctagg ctgtaaacgc tcggcaaaat tactccgggg cgatagattt
       61 gtacgatccc caaaaaatcg acgaggtcga ccagcgcgac caccgctatt cctccagcca
      121 gacaaaccaa cagtccccag cgatcagcgg ttttgataaa gaaaaagacc gtgatccctg
      181 cgatcatcga aacccaccag tattgcagaa tcgaatctga atccagaatc gctggcaaac
      241 cggtcatgct ctcgagcatt ttcaccatgc aaagtgccga gaacaaaggc gcaaagaaca
      301 gcgcaacaac cagcttccgg ctgttcatat aaagaagccg cagcgcatat tagtaattca
      361 atctgacccg gttaaatccc gttcgctcag ctaccacggc aagccattaa ccgtagacaa
      421 aatcagcgcg acggctcccg gcgtcgaagg tttgcgccga tcgcaccctt aaacatctca
      481 gcgaagaatc cacccagcgc acccgccggc atcgcggcag cgaaagatag cgccatgaac
      541 cactcgggga attgattctc cgaatccccg gaatgcatcg ccaggccaac aaggatcgag
      601 gtaagaccga ccagcgcgcc atgcttcatc tccaaggctt tggctttgta acccgcgtag
      661 aacccgccca tgagcgtacc gagggaaccc aacaccagac cggctgcaag accatcactg
      721 ctgatccaga acttcgccgc ttcttcctcg aaggccgccg cagcgccaga gtttggcgcg
      781 acgtaaaagc cgtagtaaaa agcagccagg acaaagctcg cgatgaaatc gacaatcgcc
      841 ccggtaagta tcggccggaa ttgaataccc gtgaaaaatc ctgtcggctc gatgggcttc
      901 ggcggagtac ctgttggttg gggatcgtat tgcataggaa atttttctgc taaggtcgac
      961 gctgattatg aatttgccgt cgactcaact caacaaaata accgtacact taggccaaag
     1021 gcaaccgatc cgataaaatg tttaatcgtt acgactatga agccgagttt tatcccagtc
     1081 tgaaccgctt gccgcttgat gtgcgtcgca aactcgatct caccggcatc aagatttcac
     1141 tgagagactg gctcgccatc agcctggaag agcggctcgt gctttgtcac tttccctgtg
     1201 aaacagacga agagcgcaag gttttcatag cctacttgga ttttctcgcg ggaaaatatc
     1261 tggctagatc gagcgcaaaa accgcaccca tggacagcgc cctgtggggc gaattgaacg
     1321 tgccggatgc agtcgtgcag aggagcatgg caatggatca agtggttcgc gcaagcgaat
     1381 ggcggcgttg gccagcgcac catcgttatg ctctgtacaa atcagcagta tctaacaagc
     1441 aacctgaagc atttgagcag gtattgttgc aattgcgcaa actggcagcg aatgagtgac
     1501 gccgtcagag cgcgatgaaa ctcagcgacc gctggctgac aacggcggga ttggctgcat
     1561 cgcgcggcga acgcgaatcg gagagatttg atgtcttctt tactgatatt tctagccatg
     1621 gtggccggga cagctctaat cggaatccag ttcggccccg acgcgtggta ccaggcactg
     1681 cagaaacctt cgtggacacc gccgagttgg ctcttcgctc cagtctgggg cgggttgtat
     1741 attgctatcg ccgttgccgg atggttggtg tggcgatcca aggcgcttag cgttaccaag
     1801 cccatcctgc tttggctcct gcaattgatt cttaacggac tatggtcatg gctgttcttc
     1861 ggtttgcatc ggccggacct cgcggccatc gatatcatcg ctctgcttgt cacgatctgt
     1921 ttttttatcg ccagcgcaaa aaaaatcagc gccatcgccg cttggctgtt cgtcccctac
     1981 gcgttatggg tcggttttgc cgcggcattg aacttttcga tctggcagtt gaattagcgt
     2041 aattacaatc cagatgggta cggataattt atgaccgaca atctttttcc ccgcttctcc
     2101 gaccgcgaat atgcccgtcg gtacagtacc gtgcgcgccg cgatgtcgga agacaacatc
     2161 gacgcgctgg tgattttcgg cgcccgcggt tcctccgagg tttcctattt agcgaactat
     2221 ctgccgcagt ccccgtgctg gctgctgttg ccgcgcgacg gcgacgcgac ggtattcatc
     2281 cactttttta atcaccaacc ctgcgccaaa gcgcagtcga tcatcgacga cgtccgctgg
     2341 tacggtccga caccgatgcc aacgctcacc gaagagatta ccaagcgcgg attgtcaaac
     2401 agcaagatcg gtctggtcag catgcgctcc atggcctacg gccacgtcat ggaattgcag
     2461 cgccagttcc cgaacgctca atttgtcgaa tttggcccgc agttcggcaa aattagaaga
     2521 gtacgcagcg aagaagaatt ggtttatctg cgcaagagcg gctatctcac cgatctcgcc
     2581 tgcgaagctt tagagaacaa cctgcgccca ggcttgaccg agcaggatgt cctggcgatc
     2641 gtctacatgg cttatttgaa aaacggcggt gatcccggca tccactttat cgctaccacc
     2701 aacatggacg ctcccgatcg ttttgtgccc tggcagcgcc agagcttccg cgtgctcgaa
     2761 aggggcagcg tggtgattac cgaattgacg gtcagctatt ggggctactc gacgcaaatt
     2821 catcgcccgt tcgccatcgg caaggagccc acaccgacgt atcgtaagct cttcgacgcc
     2881 gcctatgatt gctacgaaag cgtgcgtaaa atttgcaaac ccggcaccac cagcgaacag
     2941 atcgtcgcgg cgacatgcgc catcgaagcc aacggcttta ccagctatga cagcgtcttt
     3001 catggcgaag ccggcaaatc cccggagctc ggcaccagct cagcggcaca tccgctagag
     3061 cgatggacgc tggaagaaaa catggtccac gtcatccaac ccaatccagt aacaaaggat
     3121 ttcaaatcgg gcctgcaact cggggcggcg gtcgtcgtta aaccgcacgg tggtgaagct
     3181 ctacataact atccgttcaa atttccggtt tgcggttaag gagagcaaat gaattcgtct
     3241 caaacagaaa acataaaatt tcttgcattc gacgtcttcg gcacagtcgt cgattggcgg
     3301 tcgagcgtca tcgccgaagg tgagcagctc ggcaaagcaa aaggaattaa cgttgactgg
     3361 acggcatttg ccgatacatg gcgttcgatc taccgcccgt tcatggataa ggtccagaat
     3421 ggcgagctgc catggacaaa gctggacgac ctgcaccgga tgatgctcgc cgagacgttg
     3481 gaaaaattcg ccatcgacgg cttgaccgtt gcggagaccg aacacctcaa ccgtgtctgg
     3541 caccggctca aaccctgggc ggattctgtg gctggactgc agcgcctgaa gtctcgttac
     3601 ctcgtcgcgc cattgtccaa tgggaatatt tcccttttga cgaatatggc aaagaacgcc
     3661 ggtctgccat gggactgcgt tttgtcggcg gaaaacgtcc atcgctacaa acccgatccg
     3721 tcggtctatt tactcgcgcc gcaactgttc gatttgaaag ccgaggaagt catgatggtc
     3781 gccgcccacg agcatgatct ccaatccgct cgcaaacacg gactgcgcac cgcctatgtt
     3841 catcggccgt tggaacatgg acctggtaaa gcgtccgaag ttccgccgcg ggatcgttac
     3901 gatatcgtcg ccagcgattt tctcgacctg gccaagcagt tgtcggcata accgaaggag
     3961 ccgttcgtga tcgcgccaat cctcgccgtt gctcttatac tgactctgtc gtttgcggga
     4021 atctcctccg ccgacgccca acgcatccgt gcggctagtg gcgggctgtc gatcatccac
     4081 tccttactgt gggtcaccta cgagcaaaaa cttttgaaga agtatggcct ggatctcgaa
     4141 tatatcgcca ttgagaacgg cactgtcggg atgcaggcac tgctcgccaa cgaagcccag
     4201 tttctgtttt ccacctcctc cctcgcggtc aatgctaatc tgcgcggctc ggatgtcacg
     4261 gttgtcgccg gtgggttgaa ctttattccg gacaaactga ttgtccgtcc ggaaattact
     4321 aaacctgagg aactcgccgg caaacgcttg gcaatcagtc gctttggttc ttcgtcggaa
     4381 accagcgcca aactgacgtt ggagaaaatc ggtgtgaagc ccgagagcgt ttcgctgata
     4441 caacttggcg gggtttcgac ccgccaggtg gcgctaatgg ccggacaggt tcaagcgact
     4501 gtgctcagtg acccgcaggc gaccgccgca accactgccg gcatgaaatt atgggtcgac
     4561 ctgtcggaga gcaaatgggg cctgccccgg atttgcttca attgctttat ggccaagcgc
     4621 agctttctcg acgcgaaccg cgagaccgcg agcaactttc tcaaagcggt tatcgaaggc
     4681 ctctacctgc tcaagaaaga caaacccctg ggtgtgcgct tgatcaaaaa gtatctccgc
     4741 ctgagcgacg aagcggctgc cgtcggctat gatttttaca tcggcaaaca tggcgaggga
     4801 atgctcagct tgccggagcg ccgtggcatg gaattcatca tcgccgaagt gacgaagacg
     4861 aatccggcgg cgagcaaagc gactcccgaa agcctccgtt tgctggagcc gagtttttta
     4921 gatgaaatca aaaagagtgg cttgatcgag cgcgtgaaat aaaattttaa cgatgggcgc
     4981 agcaaactca gccttcgccg gcattgaatt tcaatagtac ggactggttt caactctcgt
     5041 atggcagaac accgtaggcc aaccgaacca ggcggaccac atcgccatcg tctccgtgcg
     5101 ctcgtgcaca agcccgccgg cggcgggcgt atgcagttcg acaaagatgc gctgaaggat
     5161 ctgatcgttt tcctgggcct gcctctgctt gccgtgattg cagccttttg gtttgccgcg
     5221 cgcttcgttc agccggcgcc accggatcat tttgtcatga ccaccggtgc agacggcgga
     5281 gcttaccatc tgttcgcgca gcgttatcga gacatcgtta aacgcgagaa tattacaatt
     5341 acgctcaagc cttccgccgg ttccatcgaa aacctgcagc gcttgcagga tgataaatcc
     5401 gaatttgacg tcggcttgat ccaagccgga gtagccggtg gcgtagcgcc aaccgctctg
     5461 cgctcgttgg gcgcggtctc ttacgaaccg ctctgggtct tctatcgcgg cgccgataac
     5521 atcgagaaat tgagccagct cgccggcaag cgaatcgcag tcggcccgga agggagcggc
     5581 acccgggcgc tcgccttgca gctcctcaaa gtcagtggca tcgatccatc ggccccagag
     5641 ctagtgcctt tcggtggtaa cgaagcggtg aaagcgcttc ttgatgaaga catcgaagcg
     5701 gcgattctag ttgcatctcc cgatgctccc accgttcgag agctggccaa tgccaaagac
     5761 gtcaagctcg ccagcctgat tcaagccgaa gcattcacgc gacgctttcc atttttgacc
     5821 gcgctgcagc tgccccgcgg cgccatcgat ctcgccaatg atttgccggg acgcgatgtc
     5881 accttgttgg ctactacggc aaatttggtt gtcaaagaac atttccatcc tgcgctaggg
     5941 tacctcttgc tccaggctgc ctcagaagtg cacggccgtg ccggagtatt gcaaaaatcc
     6001 ggggagtttc ccgcaccgcg cgagtcggaa atagcgatcg ccgcagaagc gcaacgatac
     6061 ttcaaaagcg gccagccgtt cctgcaacgg tacctgccgt tctggatggc gaacctgatc
     6121 gagaggttgg cggtgctgct gctgccgttg gttgccgtgg tgctaccgct gttcaaaata
     6181 ctaccggtgt tgatccaatg gcgtaacaag tcgcgggtgt ttaaatggta cggcgaactc
     6241 aaatacctcg agacccaagt tgccaccaac gccgaccctg ctcctcagct cgaccgctac
     6301 ttcgagcgcc tcgatgagat cgaagacggt gtcagtcaca ccaggatcgg ggccagctat
     6361 tccgactatg tttacaattt gcgcctgcac attgagctgg tgcgcaacca cctgcaccgg
     6421 ctggaagaaa gagcaagtga aaccaaggct cacggtcaga tgtgatcgga cgttgggcgc
     6481 gtgtccgttc cgcttcggca gcgccatcgg cgtcatattg gtttgtctct catgcactga
     6541 accgacagga caaatagccg ctggtccgca gcgcgaatat cagcagcagc gccaaaccga
     6601 tgaaaatggc gttgaaatgt tgctggcctc agcgccaggc tcacgtttcc agctcggcga
     6661 gcagaatcct atgcgcgcga gcaattttgc gatcgaacga aagactcagg cagcatcgcg
     6721 catcgatggc gcgctgcaat actggaatgt gacggcccac gatctcgact acacttcggg
     6781 cggctccggc aaaaccattc ggctgcatat ctacgccggc cgcggccagc aagtccatac
     6841 atggaagaac cagaaaggat ttcttgccac gccatctgat attcgcaatc aagagtttac
     6901 tgcctttgtg cgagtccata acatcagtga tccaggacgg gcagcaatta cactgaagat
     6961 ccgcggtggc gcacacaccg ataagaatcc cgacctcgct tcctgcacga tgatagcatt
     7021 tcaggcaaac agtactggag cggtcgcgcg cttcggcaaa gagctcaccc accccaacta
     7081 tgattatgtg actttaacgc cggcgttcga cgcggggtta ctcgagaacc gttgggtcgg
     7141 cttaaaactg ctgagctata gctcaccggg tgagccaagg cgcgtcacca atcggctcta
     7201 cctagacatc gatccattcg aacacgtagg cgccaagccc cgcaataact ggaaacaatt
     7261 cgccaagtat gtcgatatcg aaggcgtaag cacgggcaag tacaacaaac tcgcggactg
     7321 gggcggctgg caaacaacgt tgcggagcga cggcgtcagc agtttagatt tcgcgcttat
     7381 cagtctgcgt gaggtccagc ctggccttta atagttgccg cgagccgttg gtaaatttcg
     7441 cgtgggtcca ggtaaattta ccgccgcgcg gtaagttgac tagatttctc gctgcgccag
     7501 actgtgtgaa aactcggtgt ctctctgatc tgtcatctcg cacggcgtga gagatcttgc
     7561 attttgtaca catagaaaga caagatttcc tccgcttcgc gtcgaaatga caattaccac
     7621 acagtctggc gctcgaaatg attggcatgt tccattcgat cgatttcaga cattctactg
     7681 gcgacgtgtg tagaagcgcg ggcccaacgt agtgggcggc gcgttgcaaa tccgtgcctt
     7741 tgcgactctt gcgtcctttg cggccaacat tcggtcagcg catctggcac accacgtcga
     7801 ccagcgccgg cttgcctgac ttgacgactt tca
//
LOCUS       DATLCH010000054         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8283320, whole genome shotgun sequence.
ACCESSION   DATLCH010000054 DATLCH010000000
VERSION     DATLCH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8283320"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VMZ02_01530"
     CDS             <1..1146
                     /locus_tag="VMZ02_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HUR70635.1"
                     /translation="AAAIEAVASNGGQIMPPVMGAAAFLMAEFLNIPYGQVALAAAVP
                     ACLYYLALFTQIDLESAKRGLLGLPADQIPKFRSVIRLGWVFLIPLSILVYTLMIENW
                     EAGKAGMVAVIGVFVVGALQKETRPSFKKIVAAFEETGKILLDITVITALAGIVIGAL
                     HLSGFTFKISLLLVTLSGNNVFLLLLITALGCLFLGLPLPTTVVYITLAVLAAPALVQ
                     LGIPPLAAHLFLFYFGMVSLITPPDCLPVYIAASIARANFWQTGWTAMRLGIAAYFVP
                     FIFALHPPLILMGTLKEILIAIITASIGVILLGAGCAGYLFRPLSWINRGLLWLAALL
                     LLLPSTSNLLLFADFAGFALGAAILAWEWSQRDHSSKMITESGTITR"
     gene            complement(1239..2255)
                     /locus_tag="VMZ02_01535"
     CDS             complement(1239..2255)
                     /locus_tag="VMZ02_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70636.1"
                     /translation="MSGRFWLIAWIVASLIVTLNGTPTLAQTKITIGVAAMSPRTIPL
                     FIAQEQGLFSKQAIDARIVLIKGAPILVASLISGDLEVGYTGGTSVLGAAAQGPYLKI
                     LSSISSKLTHTMLASPNLKTAEQLRGKRFGIQSMGGTTWMHTILGLEHVGLDVKRDNI
                     SILMIGDSVLIGQALESGRVDAAVLDGALARRLINKGFSVIVDLTPFNIPMVNQAVVV
                     APDFVQKRGELAERILMVLVDSLAFTLAPVNKSIVVRTIMRRLQIDDPKVGEEGYQDL
                     LTSIDRKPFPSLEGLRNIRRLMATQNPKAANVKVEDLIDVRLIRKLDENGYMDKVAAG
                     YGLK"
     gene            2669..3406
                     /locus_tag="VMZ02_01540"
     CDS             2669..3406
                     /locus_tag="VMZ02_01540"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HUR70637.1"
                     /translation="MAPQSGGFMRTFGTAMLGGFLGSMLFSGLAHGGFGGLGGFGSSG
                     IGFVEILLFAGLGYFLYRKFLRPAAATGYGTTQYQNTRDYSPQYTPVQEAPAINSDID
                     YRSLTMMDRSFDPNQFLKTAQDLFFKVQGAWNKQDATALRSLCGSELMQTWEQELNDL
                     RARGHQNKMESIALRESEITEAWTENGQDYITVRLHANLLDYTVDQKSGNVVSGNNSD
                     PIEFQEYWTFTRPVGPNPWKLTAVQQA"
BASE COUNT          771 a         1039 c          961 g          893 t
ORIGIN      
        1 gccgcggcga tcgaagcggt ggcatctaat ggcggccaga tcatgccgcc ggtcatgggt
       61 gccgcagcat ttttgatggc ggagttttta aacatcccct acggccaagt cgccctcgcc
      121 gcggcagtcc ccgcatgcct ctattatttg gctctgttca ctcagatcga tctggagtcc
      181 gccaaacgcg gcttgttagg attaccggct gatcagattc ccaagtttcg cagcgtcatc
      241 cgtttgggct gggtgtttct gattccgctt tcgattctcg tctacacact gatgatcgaa
      301 aattgggaag ccggcaaggc cggcatggtc gcggtcatcg gtgtctttgt cgtcggcgca
      361 ctgcaaaagg aaacccgccc gtcgtttaaa aagatcgtcg cagctttcga ggaaaccggc
      421 aaaattctgc tcgatatcac agtgattacc gctctcgccg gcatcgtcat cggcgcgctg
      481 catctttccg gcttcacttt taaaatttca ctcttgctcg tcacgctctc cggcaataac
      541 gttttcttac ttctgctcat cacagcgctt ggttgtcttt ttcttggttt gccgttgccg
      601 acgacagtcg tctacatcac gctagcggtt ttggcagctc cagcgttagt gcaattaggt
      661 atcccgccgc tcgcggcgca cttgttcctc ttctacttcg gcatggtgtc actaataacg
      721 ccgcccgact gtttgccggt gtatatcgca gcatcgattg cgcgagcgaa tttctggcag
      781 accggctgga ccgccatgcg cctcggcatc gccgcctact tcgtgccgtt tatcttcgcg
      841 cttcatccac cgctgattct catgggcacg ttaaaagaaa ttctcatcgc gatcatcacc
      901 gcatccatcg gcgtgatcct gctcggcgcc ggttgcgccg gctatttatt ccgcccattg
      961 agttggatca atcgcggact tctctggttg gccgctctgc tcctgctcct tccttcgacc
     1021 agcaatctgc tccttttcgc cgacttcgcc ggcttcgctc ttggtgcagc tatccttgcg
     1081 tgggagtgga gtcaacgtga tcacagttcg aaaatgataa ctgaatctgg cacgatcact
     1141 cgctaaaaaa cgaaattccg gcaatcaggt ctttcatcaa atgcctgaca cgattcttgt
     1201 gctgacttag tttttagctg caacgacatc gatcgtattt acttcagccc ataccccgcc
     1261 gccactttgt ccatgtaacc gttctcgtcg agcttgcgaa ttaacctgac gtctatcaga
     1321 tcttcgacct tgacgttcgc cgctttggga ttttgcgtcg ccatgagccg gcgaatattg
     1381 cgcaggcctt cgagtgaagg gaacggcttg cggtcgatac tggttagaag atcctgataa
     1441 ccttcctcgc caaccttcgg gtcgtcgatc tgcagccggc gcatgatcgt tcgaacgacg
     1501 atacttttat tcaccggcgc caacgtaaac gccaggctat ccaccagtac catgagaatc
     1561 cgttcggcga gctccccacg tttttgcaca aaatcggggg ccactacgac ggcctgattg
     1621 accatgggaa tattaaacgg cgtgagatcg acgatgaccg agaaaccttt attgatgagc
     1681 cggcgcgcca gcgcgccgtc gagcaccgcg gcgtcgaccc ggccggactc gagcgcctgg
     1741 ccgatcagca ccgagtcgcc gatcatcagt atgctgatat tgtcccgttt gacgtcgagc
     1801 ccgacatgtt cgagcccgag gattgtgtgc atccaggtgg tgccgcccat gctctgaatg
     1861 ccgaagcgtt tgccgcgcaa ttgctcggcg gttttcaggt tcggactcgc tagcatcgtg
     1921 tgagtgagct tgctggaaat agaggagaga atttttaagt atggcccctg cgccgctgcg
     1981 ccgagcacgg acgtgccgcc ggtgtaaccc acctcgagat ctccggagat gagactggca
     2041 accaagatcg gcgcgccctt gatgagcacg atgcgcgcgt caatggcttg cttggaaaac
     2101 agtccttgct cctgcgcgat gaacagtggg atcgtgcgcg ggctcattgc ggcgacaccg
     2161 atggtgatct tggtttgggc gagcgtggga gtgccattaa gagtgacaat cagggaagca
     2221 acgatccaag cgataagcca aaatctgccc gacatatttg tcctccttcg ctcagcagtc
     2281 actgagaatg tggcaaacat ccggtattgg aaacagacta ctcatacgag aaacggtgtc
     2341 ccggcgtcaa atcattctat ggggcctgga ttgattgacc gatctcttta agctaagctt
     2401 cccacaattg cagttcgatt tgacccaagg aggcaaaatt cttatggcga tatcgaaacg
     2461 tagaataacc tttatggcga ttagcttatt cgcctttgtc ggcttatcgc aaattgtcct
     2521 tgagagtttc gtcgacgctc gcgccggcgg cggcagctcc gctggctcgc gcggctccag
     2581 gagctatcag tctccaagca ggtcctcgca accaaatcct tcacaaccga gacgagaagc
     2641 agctccccca ccgcagcagc ctagcccaat ggcgccgcaa tccggcggct ttatgcgtac
     2701 ctttggcacc gccatgttgg gcgggttttt aggctccatg ctcttttccg gcttagctca
     2761 tgggggcttt ggcggccttg gcggctttgg cagtagcggc atcgggtttg ttgagatcct
     2821 gctgttcgcc ggtctgggat attttctcta ccgcaagttt cttaggcccg cggccgcaac
     2881 gggttacggc accacgcaat accaaaacac ccgggattac tctccacaat acaccccggt
     2941 tcaagaagcg ccggcgatca atagtgatat cgattaccgg tccctcacga tgatggaccg
     3001 aagcttcgat cccaatcagt ttttaaaaac cgctcaagat ctctttttca aagttcaggg
     3061 cgcgtggaac aagcaggatg ccaccgctct gcgttcccta tgtggatccg agttgatgca
     3121 aacatgggaa caggagctga acgacctacg ggctcgcggt caccagaaca aaatggaaag
     3181 tatcgccctt cgggaaagcg agatcaccga agcatggacg gaaaacggcc aagactatat
     3241 taccgttcgt ctccacgcca atctgctcga ttatactgtc gatcaaaaaa gcggaaacgt
     3301 ggtcagcggt aacaactccg atcccattga gttccaagaa tactggacct tcacccgtcc
     3361 cgtgggccct aacccatgga agttaacggc ggtgcagcag gcatagcagg tcatcgataa
     3421 ggaatattca aaccagcggc gcacgctctc gtgcgccgct gtgcttttgg ccgtagagaa
     3481 gccggttcaa ggatcaaaag cgagaagtaa tctttccttc ttgattccat atagacgagc
     3541 gcggtgtgtt tgaaaggaga cgatggcatt ggattacggt gtgcatcttc cagtcattga
     3601 cttcggattc caacgctttt cattggagcg gttggcggca tacgtgaaaa ccgctgccga
     3661 actg
//
LOCUS       DATLCH010000055         6634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2417398, whole genome shotgun sequence.
ACCESSION   DATLCH010000055 DATLCH010000000
VERSION     DATLCH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417398"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VMZ02_01545"
     CDS             <1..350
                     /locus_tag="VMZ02_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70638.1"
                     /translation="TLWANVQKGTVPFYYMGRGSIQDPSAALHQYFHSGETPRIGYVN
                     PKVDELLDKEQQEFDPKKRRQYLSQAMSLITEDAPACFMWRHKLLWGLHNRVDYKPLP
                     DSRIYAIDMAVKK"
     gene            476..1477
                     /locus_tag="VMZ02_01550"
     CDS             476..1477
                     /locus_tag="VMZ02_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70639.1"
                     /translation="MVSYLFRRLAGTLPVLVLISLLVFLLIHAAPGDPTLMLLGEETN
                     AAEVAKAKARWGLDQPLYVQYWKFLQSIAAGDFGKSFKYAEPVTSVIRSRLPATIELA
                     IFAIVIATLLAIPLGVWAGARPNSWIDNLGTTCGLFGISMPSFWLAIMLILLLAGVLN
                     ILPTSGRSTYGVAGPEQTGFYILDSLMQKNWPAVWDALTHLFLPALALGVNMLGILMR
                     VTRSSILEVMNEDYVRTARAKGLREKKIVWRHVSSNALIPVITVVGLELGTLLSGSII
                     VETVFSWPGGGSLLITALNARDYPLVTGLVLTYTTAFVAINLIIDGLYAVVDPRIRY"
     gene            1446..2342
                     /locus_tag="VMZ02_01555"
     CDS             1446..2342
                     /locus_tag="VMZ02_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70640.1"
                     /translation="MQSLTLESVTEKNPGALVRLLPRLHAKAFTGGLIVLTFILVGIF
                     GPYVAPHDPNKQELTAMMRAPEGIGSSHVLGTDNLGRDIFSRVIHGSRVSLLIAFAVV
                     FISGLIGITLGAVSGYFGGKVDFAIQKLVEVIWAFPPLLLGITIVAFLGQGLFNLILA
                     LVAQRWIPYCRVVRGQALSLRSRDFVVAARSLGSGHGKIIVRHIMPNLIQTSLVIGTF
                     AMASSIIAEASLSFLGVGVPPHIPTWGTMLADARIYISTAWWLPLFPGLCIFITVLGI
                     NLLGDALRDILDPRLKRTGLGV"
     gene            2477..3880
                     /gene="pyk"
                     /locus_tag="VMZ02_01560"
     CDS             2477..3880
                     /gene="pyk"
                     /locus_tag="VMZ02_01560"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HUR70641.1"
                     /translation="MRRTKIVCTIGPASSSEKMIEGLIRAGMNVARLNFSHGDYASHR
                     RVIRTIRKLERKLGMPVAILQDLPGPKIRIGAMAGDRVRLQTRGRLVLTTRKVLGSEL
                     VVPVNFPGLTHAVKKGDPILLGDGEIELEALQVGKQEVRCRIIVGGILGSHKGIHFPR
                     SSLNIRALTAEDKKDLAFGIENKVDMVALSFVRNSQDILYARREMHKLSANIPLIAKI
                     EKHEALDNLDSILQNVDGIMVARGDLGLEIAQQRIPAMQKRMIREANHLGKPVITATQ
                     MLRSMVWNPRPTRAEVADIANAILDGTDALMLSEETAAGDYPLEAVKIMAQVAEETEM
                     ILEPRERFQGLPKTVPEAISVSAISLARDLQVEAFLIPTTSGNTARLIARYRPKQPII
                     AVSPDPRTVKMLCLVWGVYSTPSRSFKSTDEMVQVLQRKALESGIVKRGDLVAITAGL
                     PLHQAGTTNMITAKVID"
     gene            complement(4005..5495)
                     /locus_tag="VMZ02_01565"
     CDS             complement(4005..5495)
                     /locus_tag="VMZ02_01565"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HUR70642.1"
                     /translation="MNRPLNTTIIFRFAVIAIGSLLIIGGVDAASVNQRDYWGGRYQS
                     VPHTDSQRPRARFAIDAVRRWSGIMLDANAADHALPIPNQLGPLRTARAFAIVQIAIF
                     DAANAIAGVYHNFTDMNQVAGPASIEVAVAQAAHDTLLMLFPLQSAKFDTLLSEELRK
                     MPDTPTKRNGIDLGRRVAAAILAARANDGSQYPEELYGTEYLPAMEADTWRQDPISVI
                     PIALGSRWGELVSPFVLPSADQFPIPPPPVAGSAEFIAAFEEVKNYGGCGSDPRACSI
                     AAEGSVTDTTRTSEQTQIGVFWGYDGTPGVGTPPRLFNQIALQIAQQKNSSGLELARL
                     LALINVAMADAGLAAWKSKYEYEFCRPITAIRQAGFTTWTPLGAPASNKIGPNFTPPF
                     PSYASGHATFGAALFQILRRFYHTDRIPFDFESDEFNGVTKDNTGVVRPRLSRSYANL
                     SQAEEENGQSRIYLGVHWAFDKTAGIAQGREVANYIIDHVFTPQSR"
     gene            5698..>6634
                     /locus_tag="VMZ02_01570"
     CDS             5698..>6634
                     /locus_tag="VMZ02_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70643.1"
                     /translation="MSEALKQFEEELQSKNFKGYWQNQQGDLARQPVASFDACHWKGK
                     DLFAAMEKAGDVVGLDVSFRRVIQLWNPALKNGTSRTLVLNLQLLKPGENAGSHRHIA
                     GAIRFILKGHGTRLIVEGESFEIGEGDFVTTPSWTWHDHENRGESMMWLDGLDAPLVR
                     LLETDFHEPDPRKKQTVTRPDGYTLATTGPLRPAWVKPNTIQPPAFCYKWADTESALN
                     KVGEQPGDAFNGIVLDYANPLNGGPTLPTMNCQIHMLRAGEKTKTHRHTSSDIYHVWR
                     GSGVSYIGDKRFEWEQGDSFVVPLWQNHRHENTSKD"
BASE COUNT         1483 a         1926 c         1785 g         1440 t
ORIGIN      
        1 cgacgctctg ggctaatgtc cagaaaggca cagtgccctt ctactatatg gggcgtggtt
       61 cgattcaaga tcccagcgca gcactgcatc aatatttcca cagcggagaa acgccgcgca
      121 tcggctacgt caatccaaaa gtggatgagc tgctcgataa ggaacaacaa gagttcgacc
      181 cgaaaaaacg ccggcagtac ttatcacaag caatgagctt gatcactgag gacgcgccgg
      241 cttgcttcat gtggcgccat aagctgctat ggggattgca caaccgtgtc gactacaagc
      301 cgctgcccga ttcacgcatc tacgccatcg atatggcggt taagaaataa cgctggaact
      361 ctgtttcagc ttagtttcgc aatccaaggc agctcggtta tgagctgctc accgtaacaa
      421 gtcatcaagg ttcgacgcga gcttggtttt tcaaatgttt gaactttgaa cgattatggt
      481 cagctacctg ttccgccgcc tcgccggcac cctcccggtt ctagtgctaa tcagcctttt
      541 ggtatttctg ctgattcatg ctgcgcccgg cgacccgact ctcatgctcc tcggtgaaga
      601 aactaacgcc gcagaagtcg ctaaggccaa agcgcgttgg gggctcgatc aaccgcttta
      661 cgtgcagtac tggaaattct tacaaagcat tgccgccggc gacttcggca agtcgttcaa
      721 atatgccgag ccggttacga gcgtgatacg tagccgactg cccgcgacca tcgagctggc
      781 gatttttgcc atcgtcatcg ccaccctact ggccatcccg cttggggtat gggccggtgc
      841 ccgccccaac tcatggatcg ataatctcgg cacaacgtgc ggcctcttcg gtatcagcat
      901 gccaagcttt tggctggcga tcatgttgat cctgctactc gccggcgtgc tgaacatctt
      961 acccacttcg ggacgaagca cctacggcgt ggccgggccg gagcagacgg gcttttacat
     1021 tctcgatagc ctaatgcaga aaaattggcc cgcggtttgg gatgcgctaa cgcatttgtt
     1081 cctgccagcc ctcgccctcg gcgtcaatat gctcggcatt ctcatgcgcg tcacccggtc
     1141 gtcgatcttg gaggtcatga acgaagatta cgtacggacg gcacgcgcca aaggcttgcg
     1201 cgaaaaaaaa atcgtttggc gccacgtcag cagcaatgct ctgattcccg tgatcaccgt
     1261 tgtcggcttg gagctcggca cactgcttag cgggtcgatt attgtcgaaa cggttttttc
     1321 ctggccggga ggcggcagtt tgttgatcac cgcgctcaac gcccgcgatt atccgctagt
     1381 caccgggctg gtgttgacgt ataccacggc tttcgtcgcg attaacttaa tcatcgatgg
     1441 cctttatgca gtcgttgacc ctcgaatccg ttactgaaaa gaaccccggc gcgttggttc
     1501 gcctgctgcc gcggctgcat gccaaggcgt tcactggcgg gctgatagtg ctgacgttca
     1561 ttttggtcgg catcttcggc ccgtacgtcg cgccgcatga tcctaacaaa caagaactga
     1621 cggcgatgat gcgagcaccc gagggcatcg gctcgagtca tgtcctcggc accgacaatc
     1681 tcggccgaga cattttcagc cgagtgattc atggttcgcg agtatcactc ctgatcgcct
     1741 tcgctgtcgt atttatatcc ggcctgattg gcataacgct cggggctgtc tccggctatt
     1801 tcggcggcaa ggttgatttt gccattcaaa aattggtcga agtcatttgg gcgtttccac
     1861 cattattgct cggcatcacg atcgtcgcgt ttctcggcca gggacttttc aatctgattc
     1921 ttgcgctcgt ggcgcaacgc tggattccgt actgccgcgt cgtgcgcggc caagcgctat
     1981 ctctgcgctc tcgcgatttt gtcgtcgcgg cgcggtcttt aggttccggt cacggcaaaa
     2041 tcattgtgcg tcacattatg cccaatctga tccaaacctc attggtgatc ggcacgtttg
     2101 cgatggcgtc gtcgatcatt gctgaagcca gcctgagttt tctcggcgtt ggcgtgccgc
     2161 cgcatatccc cacctggggc acgatgctcg ccgacgcgcg tatatatatc agcaccgctt
     2221 ggtggctgcc gctgttcccc gggctgtgca tcttcatcac cgtgctcggc ataaatctgc
     2281 tcggcgatgc tttgcgcgac attctcgatc cgagactcaa acgaacggga ttgggggtgt
     2341 gacaacaaca tcagagtgac ggaatattgg ctaatggaga gttgagattc gagcccgtta
     2401 ctccactgct cgattagttc gtcgcttcaa cgatactcgc attgattccc ctatcgtctc
     2461 gtgtaaattg ttgcagatgc gccgcaccaa gattgtctgc accattggtc ctgccagttc
     2521 gtccgagaag atgatcgaag gtttgatccg cgccggcatg aacgtcgcgc gactgaactt
     2581 cagccacggc gactacgcct cgcatcgtcg agtcattcga accatccgta aacttgagcg
     2641 caagctcggc atgcccgtcg ctatcctgca ggatctgcca ggaccgaaga tccgcatcgg
     2701 cgccatggcc ggcgatcgtg tgcggctgca aacccgcggt cgactggtac tgaccactcg
     2761 taaagtgctc ggcagcgaat tggtggttcc ggtcaatttt ccgggcttga cgcatgcggt
     2821 caaaaaaggc gatccaatcc tgcttggcga cggtgaaatc gaactcgaag cactgcaagt
     2881 gggcaagcag gaagtgcgct gccgcattat tgtcggcggc atcttggggt cgcacaaagg
     2941 catccacttt ccgcgcagct cgctcaacat tcgcgcactg actgcagaag acaaaaaaga
     3001 tttggcattc ggcatcgaga acaaagtcga catggtggcg ctatcgtttg tgcgtaatag
     3061 ccaggatatt ctctatgcgc gccgcgaaat gcacaaactc agcgccaata tcccactcat
     3121 tgccaagatc gaaaaacacg aagccttgga caatctcgac agtattttac agaacgtcga
     3181 cggcatcatg gtcgcgcgcg gagatttagg actagagatc gcacagcaac gcattcccgc
     3241 catgcaaaaa cgcatgattc gcgaagcgaa tcatcttggt aaaccggtta ttaccgcgac
     3301 tcagatgctc cgttcgatgg tgtggaatcc ccgtccgacg cgggcggaag ttgccgacat
     3361 tgctaacgcc attcttgacg gtaccgacgc gctgatgtta tcggaggaaa ccgccgccgg
     3421 cgattacccg ctggaagccg tcaaaatcat ggctcaagtt gccgaagaaa cggagatgat
     3481 tctcgagccg cgcgagcgtt tccaaggtct gcccaagacc gtgccggaag cgatcagcgt
     3541 cagtgcgatc tcgctcgcgc gcgatttgca agtcgaagcc tttcttatcc cgacaacctc
     3601 cggcaacacg gcgcgtttaa tcgcacgcta ccgaccaaag caaccgatca tcgccgtcag
     3661 cccggacccg cggacggtga aaatgctctg tctggtttgg ggcgtgtact cgaccccatc
     3721 tcgcagtttc aaaagcaccg acgaaatggt gcaggtgctg caacgtaaag cgctcgaatc
     3781 gggcatcgtc aaacgcggcg atctcgttgc cattaccgca ggcctacctc tgcatcaagc
     3841 cggcaccacc aacatgatca ctgccaaggt gatcgactag cgcatgctta gacgacgcat
     3901 cgaggcaacg gcggcattgc gttccgccgt atattttttt agttccgctt tctcaagaat
     3961 gttttacaag aagcggccag ttgggaatag cttcgatttt cggactaacg actctgaggc
     4021 gtaaaaacgt gatcgatgat gtaattagcc acctcgcgtc cctgagcgat accggcggtt
     4081 ttgtcgaatg cccagtggac gcccagatag attcgactct gcccgttttc ctcttcggcc
     4141 tgagatagat tggcatagct gcgcgataag cgtggtcgaa cgacgcccgt attgtccttg
     4201 gtcacgccat tgaactcatc ggactcaaaa tcaaacggaa tcctatcggt gtgataaaag
     4261 cgccgtaata tctgaaacag cgccgcgcca aaagtcgcgt gaccggacgc atacgatgga
     4321 aacggcggcg tgaagttggg gcctattttg ttgctcgcgg gagcaccaag cggagtccag
     4381 gttgtgaagc cagcctgacg aatggccgtg atcggccggc agaattcgta ttcgtacttg
     4441 gatttccacg ctgccaaacc cgcatcggcc atggctacat tgatcagcgc gagcaaacgt
     4501 gccaattcca agccgcttga atttttctgc tgggcaatct gtagcgcgat ttggttaaac
     4561 agtcgtggtg gtgtgcccac tccgggagtg ccgtcgtagc cccagaaaac tccgatttga
     4621 gtctgctccg aagtgcgagt cgtatccgtg acggagccct cagccgcaat cgagcaagcc
     4681 ctaggatcac tgccacaacc gccgtaattt ttcacctcct cgaacgcagc aatgaattcg
     4741 gcacttcccg ccacaggcgg cggcgggatt gggaactgat cggccgaagg taatacgaac
     4801 ggcgaaacca actcgcccca gcgcgatccc aacgcgatgg gaattacact gattggatct
     4861 tggcgccacg tatcagcctc catcgcgggt agatactccg ttccatagag ctcctccgga
     4921 tattgcgagc catcattagc tcgcgccgcg agaatcgctg cggcaacccg acggccgaga
     4981 tcgataccgt tgcgcttagt cggggtatcg ggcatcttcc gcagctcttc agaaagcagc
     5041 gtgtcgaatt tcgccgattg taacggaaac agcatcaaca atgtgtcatg cgccgcctgg
     5101 gccaccgcca cctcgatgga tgccggcccg gcaacctgat tcatgtcggt aaaattgtgg
     5161 taaacgccgg cgatcgcatt cgccgcgtcg aagattgcaa tctgaacgat ggcaaacgcc
     5221 ctcgcagtac gtagtggccc caattggttg ggtatcggca atgcatggtc ggcggcattg
     5281 gcatcaagca taataccact ccagcgccgc accgcgtcga tagcgaagcg cgcgcgcggc
     5341 cgttgggagt ctgtgtgcgg cacggattgg tagcggccac cccagtaatc acgttgattc
     5401 accgacgcag catcaacccc gccgatgatc aggagtgatc caatcgcgat gacggcgaaa
     5461 cgaaagataa ttgttgtatt caaaggccga ttcatgcggc gctccttcaa taccacgcca
     5521 taagcaatcg atgcaccatt gacaaatcgc cactcctacg gcgaatttcg cctaccaaca
     5581 gcacttgaaa catcaccacc tacagcgacg ttgtctgctg cagactacaa atctttccaa
     5641 cttgcaaccg ccgcgagaat cgcgctagat aggatgagaa ttcgccggag gtgacccatg
     5701 tccgaagcac tcaaacaatt cgaagaagag ctgcaaagca aaaacttcaa aggttactgg
     5761 cagaaccagc agggcgatct cgcgcgccag ccagtagcat ccttcgacgc ctgccactgg
     5821 aaaggcaaag atctgttcgc cgccatggaa aaagccggcg atgtagtcgg ccttgacgtc
     5881 tcgttccgac gcgtgattca gctgtggaat ccggcgttga aaaacggcac ctcgcgcacc
     5941 ctggtgttga atcttcaact gctcaaaccc ggcgagaacg ccggatcaca ccgccacatc
     6001 gccggcgcga ttcgattcat tttaaaaggc catggtaccc ggctgatcgt tgagggcgaa
     6061 tccttcgaga ttggcgaagg cgattttgtc accacgccga gctggacttg gcacgaccac
     6121 gaaaaccgcg gcgagagcat gatgtggctc gacgggcttg acgcgccgct ggtgcggtta
     6181 ttggaaaccg attttcacga gcccgatccg cgcaagaaac agaccgttac ccgcccagac
     6241 ggctacacgc ttgccaccac cggtccgctg cgcccggcct gggtcaagcc gaataccatc
     6301 caaccgccgg cgttttgcta caagtgggcc gacaccgaaa gcgcgctcaa caaagtcggc
     6361 gagcagccgg gggatgcgtt taacggcatc gttctcgatt atgccaatcc attgaatggc
     6421 ggcccgacac taccaaccat gaactgtcag atccatatgc tgcgcgccgg ggaaaaaacc
     6481 aaaacccacc gccacaccag ctcggatatt tatcacgtct ggcgcggcag cggtgtcagc
     6541 tacatcggcg acaagcgctt tgaatgggag caaggcgatt cttttgtggt gccgctgtgg
     6601 caaaaccacc gccatgaaaa cacctcgaag gacc
//
LOCUS       DATLCH010000056         4739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4657644, whole genome shotgun sequence.
ACCESSION   DATLCH010000056 DATLCH010000000
VERSION     DATLCH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4657644"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VMZ02_01575"
     CDS             complement(<1..189)
                     /locus_tag="VMZ02_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70644.1"
                     /translation="MRTLDLLASDSSHLPFLYVFKESKVMEKYGFEIDLHIVGGAKAP
                     TMAHRAKLALAGEIDFLSG"
     gene            complement(284..1240)
                     /locus_tag="VMZ02_01580"
     CDS             complement(284..1240)
                     /locus_tag="VMZ02_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70645.1"
                     /translation="MTKFFVSIILLGSTLGHVDGAMGADARKVTLSYSAISMTWFPIK
                     VAIERGFFRSEGLEPQLIQMNGNVATVALANGHVDFSLNISPVLNGAMQGLGTKLVAA
                     LNSRPFFSLVVRPEISSPADLKGKVFAVSSFGNTQAILTEKHLQHFGLKKGEYQLLAM
                     GATPARVAALEKNIAQGSLMPLPTNVQLENRGYRLLGNTAEIVAHPIAGLGVQEDKIK
                     KDGDLIKRVIRPTLRSLHLLQNNPKDTVKVLMDWTRANERDALRSLELAKPGFSKNGL
                     LTDEDLGIEWSFIQQQTKKTNVPVSVAHDMTLLREAQRELGL"
     gene            complement(1343..2173)
                     /locus_tag="VMZ02_01585"
     CDS             complement(1343..2173)
                     /locus_tag="VMZ02_01585"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HUR70646.1"
                     /translation="MEKQYTRDTLLITVAQLQNRLGSNDLCVIDTRSAEAYANGHIPG
                     ATHFDLFGLSLIDTGAAPLRAFMFMIHHILELRGVNETKEVVFYEENSGMRAARGVWF
                     LEYFGHANVKMLDGGIHAWKAAGAPVTRDATVPQAASFKTAERREVLATADDVLRSLD
                     KKDTAILDTRSAGEHFGTLVRAARGGAIPHSIHIEWTDNLDADGKFKSDADLKAMYGN
                     AGITPDKEVYSYCQGGYRAAHSYIALRLLGYPNVRNYIGSWKEWGDRTDLPLEIPKHS
                     "
     gene            2198..3316
                     /locus_tag="VMZ02_01590"
     CDS             2198..3316
                     /locus_tag="VMZ02_01590"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HUR70647.1"
                     /translation="MALQIISARKNSPRLMTSTIGLILCWLTAASFTYQLALPGRKLS
                     FPADHYSHPVFKTEWWYYTGHLATDSAKRYGYQVTFFRFGLRDRQNEIKEAPLFSELY
                     MAHFALSDLTAKKFTFRERINRGYGDSAGAATDRFVVWNGDWKVNGDGTNHDIQVNDR
                     GRQLRLVLRALKNPILHGQDGLSQKGEGVGRASYYYSLTRMQTDGELTVNGKTEKVRG
                     LSWMDHEFGSNQLREDQVGWDWFSIQLDNGTELMLYLMRRKDSSIDPYSSGTLVRADG
                     VTRKLSVKDFRIDVLDRWKSPTSRATYPMKWKVTIPAESIQLEILPAFTDQELITNRS
                     TRVTYWEGAVAIRGTAAGESVVGNGYVEMTGYAGKLTI"
     gene            3323..>4739
                     /gene="tilS"
                     /locus_tag="VMZ02_01595"
     CDS             3323..>4739
                     /gene="tilS"
                     /locus_tag="VMZ02_01595"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HUR70648.1"
                     /translation="MRFAANLLINRVRQQNLLSRGERLLLGVSGGPDSVCLLSILHDL
                     QKDYDLHLEVAHLQHGIRGEAAADDARFVGSLAEQLGVIFHLREINLPELRRRAGKGN
                     LEALAREQRYKFFAEIAQRRGLAKIATAHTEDDQAETMLMWLLRGAGRRGLGGMSPVH
                     ALNDSRRALDHGEAQIIRPFLEVSKSEILNYLHERQIDYRVDSTNHDGKLLRNWIRLE
                     LLPQLRRRIDGRVSARLADQAAMLRDEQAVLEQVAYSALENCRRNDGLDRVQLLQQPV
                     ALQRLIFRYWIERLRGDLRGIDFVHVEALRRLAATGPSQARIAIPGGWELRREYDLLY
                     LSKAQRGLKPICYCRCLTIGQTLQIPEANVELVSETLAAPVRAADADMRQACFDLQAL
                     PEPLLVRNFRRGDRFEPLGMTGQKKLKDLFIDRKVALSERSILPLLIGGDKVIWIPGH
                     GRSRSALVGPNSRAIMRIKVIP"
BASE COUNT         1020 a         1253 c         1358 g         1108 t
ORIGIN      
        1 cccgctcagg aaatcgattt cgccggcgag ggcgagcttg gcgcggtgcg ccatggtcgg
       61 ggctttggcg ccgccgacga tatgaagatc gatttcaaag ccgtatttct ccatgacctt
      121 ggactctttg aacacataaa gaaacggaag gtgtgacgag tcactggcga gtaaatctaa
      181 agttcgcatg ggtttactcc ttatatcgct ccctttgaca gtattgatgg ttgcctgtgt
      241 ttaccgcaga ttgcctgcgc tgttcaaacg ctttgagcga tccctataat cccaactccc
      301 gctgcgcttc gcgcaacagc gtcatgtcat gagcgacgct caccggcaca ttggtctttt
      361 tcgtttgttg ctgaatgaag ctccattcga tgccgagatc ttcgtcagtg agcagaccgt
      421 tcttactgaa gccaggcttg gcgagttcca atgaccgtag cgcatccctt tcattcgccc
      481 gtgtccaatc catgaggact ttgacggtat cctttgggtt attctgaaga aggtgcaaac
      541 tacgaagggt cggtcgaatc acacgcttga tgagatcgcc atctttcttg atcttgtctt
      601 cctgaacgcc gaggccggcg atcggatggg caacgatctc cgcggtattg ccgagcagac
      661 gatagccgcg attttccagt tgcacgttgg tcggcagcgg catcaagctc ccctgagcaa
      721 tatttttttc cagcgcggcc acccgcgccg gtgtcgcgcc catggcgagc agttggtact
      781 cgcctttttt caaaccgaaa tgctgcaaat gtttttccgt cagaatcgct tgggtgttgc
      841 cgaaggagct gacggcaaag actttgcctt tcagatcggc ggggctgctg atctcggggc
      901 gcactacgag cgagaagaat ggtcgtgaat ttaatgccgc caccagcttt gtgcccagcc
      961 cttgcattgc gccattgagc accggtgaaa tgttcagcga gaaatcgacg tgaccgttgg
     1021 caagcgcgac cgtagcgaca ttgccattca tctggatcaa ttgcggctcc agtccttcgc
     1081 tgcgaaagaa ccctctctca atggccacct ttatcgggaa ccaggtcatg ctgatggcgg
     1141 agtaggagag cgtgactttg cgcgcgtctg cacccatggc gccatcgacg tgccctaacg
     1201 tagacccaag caggattatc gagacaaaga atttcgtcat tcaagttcgt acattagaag
     1261 tcgattactg ccgaggctcc gaacgcagag atcggacacc gaaaacagcc ttttagaact
     1321 ctgcgtctct gcgttgaatc cattaactgt gctttgggat ctccaatggc aggtctgtgc
     1381 ggtcgcccca ttctttccag gagccgatgt agttgcgcac attcggatag ccgagcaatc
     1441 gcagggcgat gtaactatgc gcggcgcggt agccgccttg gcaataggag tagacctctt
     1501 tatcgggtgt gatgccggca ttgccgtaca tggctttcag gtccgcatcg gatttaaact
     1561 tgccgtccgc gtcgaggttg tcggtccatt cgatgtggat cgagtgcgga atcgcgccgc
     1621 cacgcgcggc tcgaaccagt gtgccgaaat gttcgccggc gctgcgcgtg tcgagtatgg
     1681 ccgtgtcttt tttatcgagt gatcgcaaca cgtcgtccgc cgtcgcaagg acttcgcgac
     1741 gttcggcggt tttgaacgat gcggcttggg gcacggtcgc gtcgcgagtc accggcgccc
     1801 cggcggcttt ccatgcgtga atgcccccat cgagcatttt cacattggca tgaccgaaat
     1861 attccagaaa ccaaacaccg cgcgccgcgc gcatgccgga gttttcttcg tagaaaacca
     1921 cctctttggt ttcgttgacg ccgcgcagtt cgaggatgtg gtgaatcatg aacatgaaag
     1981 ccctgagcgg cgcggcgcca gtgtcgatca gactcaggcc gaacagatcg aagtgagtgg
     2041 caccgggaat atggccgttg gcgtaggctt ccgctgatcg cgtgtcgatg acacagagat
     2101 cgtttgaccc gagcctgttc tggagctgag cgaccgtaat gagcagcgtg tcgcgtgtgt
     2161 attgcttttc cataggctcc ctataacact agtcgaaatg gcactgcaaa ttatctcggc
     2221 gcgaaagaat tcgccgcgcc tgatgacgtc gacaatcggt ttgatccttt gctggttgac
     2281 ggcggcaagc ttcacgtatc agctggcact accggggcgc aagctaagtt ttccggccga
     2341 tcattattcc catccggttt tcaaaaccga atggtggtac tacaccggcc atctcgcaac
     2401 cgacagcgcc aaacgctacg gctatcaggt aacctttttt cgctttggct tgcgtgaccg
     2461 tcagaatgag atcaaagaag cgccgctctt cagcgaactc tatatggccc atttcgccct
     2521 gtcagatctg actgccaaaa aattcacatt tcgcgagcgc ataaaccgcg gctatggcga
     2581 tagcgcgggc gcggcaacgg accgctttgt ggtttggaac ggtgattgga aggtcaatgg
     2641 cgacggaacc aatcacgaca tccaagttaa cgatcgtggc cggcagttgc ggctggtttt
     2701 gcgagcgctg aaaaatccga ttctccacgg tcaggatggc ttgagccaga aaggcgaagg
     2761 cgttgggcga gcatcctatt attattcgct gacgcgcatg caaaccgacg gtgagttgac
     2821 cgtcaatggc aaaactgaaa aagtccgcgg tctttcctgg atggatcatg agttcggcag
     2881 caatcagctt cgtgaggatc aggtcggttg ggactggttc agtattcagc tcgacaacgg
     2941 gaccgaactc atgctctacc tgatgcgccg caaagacagc tcaatagatc catattctag
     3001 cggaacattg gtgcgtgcgg atggagttac aagaaagctg tccgtcaaag actttcgcat
     3061 cgatgtcctc gaccgttgga agagcccaac gagccgggca acatacccga tgaagtggaa
     3121 ggtgacgatc ccggctgagt cgattcagtt ggagatcttg ccggcattta ccgaccagga
     3181 actgattacc aatcgcagca cccgagtaac ctattgggaa ggggcggtgg ccatccgagg
     3241 aacagccgct ggcgaatcgg tcgttggcaa cggctatgtc gaaatgaccg gctatgcagg
     3301 caagctgacg atctaactgt aaatgcgttt cgcggcaaat ctactaatta atcgtgtacg
     3361 gcagcaaaac ctgctgagtc gtggcgagcg tctattgctg ggcgtttccg gcgggccgga
     3421 ttcggtgtgc ttgctgtcga tacttcatga tctgcagaaa gactatgatc ttcatctcga
     3481 agtggcccac ttacaacatg ggatccgcgg cgaagcggcc gctgatgatg cgcgttttgt
     3541 tggctcattg gcggagcagc ttggggtgat ttttcatttg cgagaaatca acctgccgga
     3601 actgcgtcgg cgcgcgggta aaggcaatct ggaagcattg gcgcgcgagc agcgctacaa
     3661 attttttgcc gaaatcgcgc aacgccgagg cctcgctaaa attgccacgg cacataccga
     3721 agacgaccaa gccgagacca tgctgatgtg gctgctgcgc ggtgccgggc gtagggggct
     3781 aggtgggatg agcccagtgc acgcattaaa tgattcgcgc cgtgctttgg atcacggcga
     3841 ggcgcagatt attcggccat tccttgaagt atccaagtcc gagattctga attatcttca
     3901 tgaaaggcaa atagactatc gcgtcgattc gaccaatcac gacggcaaac ttttgcgcaa
     3961 ttggattcgc ttggagctgt taccgcagct gcgtcggcgt atcgacggcc gagtcagtgc
     4021 gcgtctagct gaccaggcgg ctatgttgcg cgacgagcaa gctgtgctcg aacaagttgc
     4081 ctattcggca ttggaaaatt gccgacgcaa cgacggattg gatcgagttc agcttttaca
     4141 acagccggtt gccttgcaac ggctaatttt ccgatattgg attgagcggc tgcgcggcga
     4201 tttgcgcggc atcgattttg ttcatgtcga ggccctgcgc cggttggctg cgacagggcc
     4261 gtctcaggcg cgaatcgcaa ttccgggcgg atgggagttg cgacgcgagt atgatttgct
     4321 ctacctgagc aaagcgcagc gcggcctcaa gcccatttgt tattgtcgtt gcttaaccat
     4381 cggccaaacg ttgcaaatcc ccgaggccaa cgtcgaactt gttagcgaaa cgctggcggc
     4441 tccggtgcgc gcagcagacg cggatatgag acaggcctgt ttcgatctcc aagccctgcc
     4501 ggagccgttg ctcgtgcgca actttaggcg cggtgaccgg ttcgaaccgt taggcatgac
     4561 cgggcaaaaa aagctcaagg atttgttcat cgatcgcaaa gttgctttgt cggagcggtc
     4621 gatactgcct ctgttgattg gcggcgacaa ggtcatctgg attcccggcc atggtcgcag
     4681 ccgaagcgcc ttagttggtc ccaacagtcg agccattatg cgaattaaag taattcctt
//
LOCUS       DATLCH010000057         6391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4658608, whole genome shotgun sequence.
ACCESSION   DATLCH010000057 DATLCH010000000
VERSION     DATLCH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4658608"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1614)
                     /locus_tag="VMZ02_01600"
     CDS             complement(88..1614)
                     /locus_tag="VMZ02_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70649.1"
                     /translation="MSNRKLSRRSFLKNSGVAGLGALLGGSIVQLSYAASRERLTALS
                     SIGLDTLHPYAHSSSPQYGIWNNMIEPLVEVDYAKKTYYGVLAESWEYQGKRWVIKLR
                     KNVRFHDSSPFTAKDVVYSINRMKNDKESLQKENFRDFVEVQAADDYTVIIITEQPVV
                     LMLDRLQNRFILSKSGMEAQGNQVELKPVGTGPYKFQTWQRDGNLVMVRNDSYWGAKP
                     SIREIVLRRVKEDSARVAGLLASQGDVTNNVPVEELSRFDNHPRIRAEKVEGVRMYFL
                     AMNVTHKPFDNKLVRQAINYAVDPTVIIKHIYEGNGYVMNGPMGSNVIGFDPKIKRYP
                     LDLNKAKELLTKAGFPNGLDVKLYFSPDRYPKAKEVCQVIADQLVKVGIKAELVSQEF
                     VIFWGKEGVNGGKLGFYYVGRPAADADTVYDQYFRSGVSPRIQYKNPEFDKLVDEEQK
                     TADAKKRLAILHQLGRILMDDVPFCPLYTLAEIYGVARNINWNARPDEKLMYAEMKIR
                     "
     gene            complement(1666..3204)
                     /locus_tag="VMZ02_01605"
     CDS             complement(1666..3204)
                     /locus_tag="VMZ02_01605"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70650.1"
                     /translation="MKKRSAVTRRQFLKESATLAAGAALGPYFLRNADAASRDRVTIF
                     HSSVADSIHPYNHSSSPIYGNWQHVIEPLIEYDVGRKDYIGVLAESWQFQGNKWTFKL
                     RKGVKFHNGATFTSKDVAFSIEKMRDEKGGSLQAPNFKDVSEVQTPDDYTVVFVTKQP
                     NAVFLDRLDNRFMLSKEAGDKLGDKLFENIVGTGPYKFVSYQRGGNLVFTRNDDYWGG
                     KAAIKEVIFKKVTEDAARLAALESGQADFINNVPDHEVARLKKHPRVRVDQLEGLRMF
                     FLAFNFAFKPWDNKLVRQAANYSVDAPAIVRNIFDGIGYPCNGPVGANVLGADPKHKR
                     YPYDPQKSKQLLAQAGYPNGCDIQLYYSAGRYPKDREVCQVVAAQMVKGGFRVELISQ
                     EWALFWDKQGVNGGRLPFYYIGRGSLTDADTLYDQYFRTGTTRRTNYSNAELDKLVEE
                     QQRTPDNKKRVQILQQAGKSIMDEALFIPLYNLADIYGVAKNLEWKKRADEKVKAWDM
                     KIKA"
     gene            complement(3223..4062)
                     /locus_tag="VMZ02_01610"
     CDS             complement(3223..4062)
                     /locus_tag="VMZ02_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70651.1"
                     /translation="MLSIRYLKLWVGGAILALLVVCGVAAPLIAPRDPQAQTLEDRLR
                     PPRWTDNGMQAYFLGTDNLGRDMLSRIVYGSRISLMVGAATVIFAGLVGCTLGAIAGY
                     FGSTADEIVSKISEIFLAFPFLLIAIAIMAFLGQGVGNLIMALMLSRWVQYCRVVRGE
                     VLSLKERDFITAAKALGAKDAYVIFRHVIPNTVASVTVIATFGMAIVIITEASLSFLG
                     LGVPVHIPTWGSMLAEGRSYINRAPWLTIFPGLAIFITVFGINLLGDGLRDIFDPKLR
                     RQR"
     gene            complement(4062..5060)
                     /locus_tag="VMZ02_01615"
     CDS             complement(4062..5060)
                     /locus_tag="VMZ02_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70652.1"
                     /translation="MNKLIRRFIGALPVLLAISFLIFLLVHIAPGDPVTLMLGDNATP
                     EDVAKLRAELGFDKPLLVQYWDFLSRAVRGDFGRSLKFGEPVIKLIAERLPATLELAF
                     ASLFIAIAIAVPVGVYSAIRHNSLLDHAGMSVALIGVSLPNFWLGIMLIYFLGGQLNL
                     LPVAGRVEYGIEIKSITGLYLVDCIITGNFDGFVSVAKHLLMPAFTLGAALAAIVTRI
                     SRSSVLEVMRQDYVTTARAKGLSEKTVIWRHILRNALITIVTILGLQLGALLSGSVVT
                     ETVFSYPGIGDLLIQSISARDYKLTQVLILMFAVTYFVVNLLVDFLYSWIDPRIKF"
     gene            complement(5120..>6391)
                     /locus_tag="VMZ02_01620"
     CDS             complement(5120..>6391)
                     /locus_tag="VMZ02_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUR70653.1"
                     /translation="VLEKQKKYLFSCVATYYDEPLVIDHAKDHSVFDADGKEYLDFFG
                     GILTVSVGHVNEKVTKAIDEQNRKFQHMSTLYANEPQVNLAEKLAQITPGRLEKSFFT
                     NSGTEANETAVLLAQIYTKCQDVITLRHSYSGRSYLALSLSAQSSWRLMPNVVPGVHH
                     IANAYCYRCPFGLTYPSCDLKCAKDAEEAIQTMTSQGRVAAFLAEPIQGVGGFIVPPK
                     EYFKEIVGIVRKYGGLFIADEVQTGWGRTGGKMFGIEHWGVEPDIMTFAKGMANGVPI
                     GATIATAEVADSMQGNTISTFGGNPVTCTAAHATINVIEEENLVENARVMGNRLRDGL
                     DALKSKYPLVGDVRGMGLMQGMELVGAQKKPDAQSTKRLMELTKNNGLLLGKGGTYGN
                     VIRVAPSLNVNKDEVDHALKVLDQSFGQLGQ"
BASE COUNT         1453 a         1781 c         1698 g         1459 t
ORIGIN      
        1 gccggcaaac gcaccgagct tgagaaattt gcggcgattg aactcgagtg catctggttt
       61 catggcggat tctccttgtt gccggcgtta gcgaatcttc atctccgcgt acattagctt
      121 ttcatcaggc cgggcgttcc aattgatatt tcgcgccacg ccataaattt ccgccagggt
      181 gtacaacgga cagaacggca catcgtccat cagaatgcgt ccgagttggt gcagaatcgc
      241 caaacgcttt tttgcatcgg cggtcttttg ctcttcatcg accagcttgt cgaattcagg
      301 atttttgtac tgaatgcgcg ggcttacccc ggagcgaaaa tactgatcgt acacagtgtc
      361 ggcatccgcc gccggccgac cgacgtagta gaagcccagc tttccgccgt tcaccccttc
      421 tttgccccaa aaaatgacga actcctggga gaccaattcc gccttgatgc cgaccttgac
      481 caattggtcg gcaatcacct gacaaacttc tttagccttg ggataacgat ccggcgaaaa
      541 atacagcttc acgtccaagc cattggggaa tcccgccttg gttaaaagct ccttggcctt
      601 gttgagatcg agtgggtagc gtttgatttt ggggtcgaag ccaatcacgt tggagcccat
      661 cggcccgttc ataacgtagc cgttgccctc gtaaatgtgc ttgatgatga ccgtcggatc
      721 gacggcgtag ttgatcgcct ggcgcactaa tttattgtca aacggtttat gcgtcacgtt
      781 catggcgagg aagtacatgc gcacgccttc gactttctcg gcgcgaatgc gcggatgatt
      841 gtcgaagcgc gacaactcct ccaccgggac gttgttggtg acgtcgcctt gactggcgag
      901 cagccccgcc actcgggccg agtcctcttt gacccggcgc aagactattt ctcgaatcga
      961 tggtttggcg ccccaatagc tgtcgttgcg caccatcacg agattgccgt cgcgctgcca
     1021 ggtttgaaac ttgtacgggc ccgtgccgac cggttttaat tcgacctgat tgccttgagc
     1081 ttccatgccg gacttggaca gaatgaaacg attttgcagg cggtcgagca tcagtaccac
     1141 cggttgctcg gtgatgatga tcacggtgta atcgtctgcc gcttgcactt cgacgaagtc
     1201 gcgaaaattt tctttttgca gactctcctt gtcattcttc atccggttga ttgagtaaac
     1261 cacgtcttta gcggtaaacg gactgctgtc gtggaagcgc acgttcttgc gcagcttgat
     1321 cacccagcgc ttgccctgat actcccagga ctcggcgagc acgccgtaat aagtcttttt
     1381 ggcgtagtcg acctcgacca agggctcgat catattattc caaatgccgt actgcgggct
     1441 tgagctatga gcgtacggat ggagcgtatc gagaccgatg ctgctcaatg ctgtgagccg
     1501 ctcgcgtgag gcggcgtaac tcagttgaac gatggaaccg cccaggagcg cgccaagtcc
     1561 ggcgacaccg ctgtttttaa gaaatgatcg tcggctgagt ttccggttgc tcatcctgac
     1621 ctccactgtg catagctaaa atcccgtcaa catttggcgg caaacttagg ccttaatttt
     1681 catgtcccac gccttgacct tctcatcggc gcgctttttc cattcaagat tcttggcaac
     1741 accgtagata tcggcaagat tgtaaagcgg aatgaacaac gcctcgtcca tgatcgattt
     1801 gccggcctgc tgtaatattt gcacgcgttt tttattgtcc ggggtgcgct gctgttcttc
     1861 gaccagcttg tccaactcgg cattactgta gttggtgcgc ctggtcgtgc cggtgcgaaa
     1921 gtactggtcg tagagcgtat cggcgtcggt caagctacct cggccaatgt agtaaaacgg
     1981 cagacgcccg ccgttgaccc cctgcttgtc ccagaacaag gcccactctt gagaaatcag
     2041 ttcaacgcga aatccgcctt ttaccatttg tgcggcaacc acctggcaca cttcgcggtc
     2101 cttgggataa cgcccggcgg aataataaag ctgaatgtcg cagccgttgg gatatcccgc
     2161 ttgagcgagt aactgttttg atttctgcgg gtcgtatgga taccgtttat gttttggatc
     2221 ggcgccgagg acgttggcgc ccaccgggcc attgcaagga tagccgatgc cgtcgaaaat
     2281 gttgcgaacg atcgccggcg catcgacgct atagttggct gcctggcgca ccagtttgtt
     2341 gtcccagggc ttgaaagcaa agttaaacgc caggaaaaac atccttagcc cttcgagctg
     2401 atcgacgcgc acacgcggat gttttttcag acgcgccact tcatggtcag gcacgttatt
     2461 aatgaaatcc gcttgacccg actccaacgc agcaagacgg gcggcatctt cggtgacttt
     2521 tttgaagatg acttccttga tcgccgcctt gccgccccaa taatcgtcgt tgcgggtaaa
     2581 gaccagattg ccgccgcgct ggtagctgac gaatttgtaa gggccggtgc cgacgatatt
     2641 ctcgaaaagc ttgtcgccca acttgtcgcc ggcctctttg ctcaacatga agcgattatc
     2701 caaacgatcg aggaacacgg cattgggctg cttggtgacg aagacgacgg tatagtcatc
     2761 cggcgtttga acttcgctga catccttgaa gttcggcgcc tgcaagctgc cgcctttttc
     2821 gtcgcgcatc ttttctatcg aaaacgccac atccttcgag gtaaaggtcg caccgttatg
     2881 gaatttgacg cctttgcgca atttgaacgt ccacttgttg ccttgaaatt gccaggactc
     2941 ggcgagcacg ccgatataat ccttgcggcc gacgtcgtac tcgatcaacg gctcgatgac
     3001 atgctgccaa ttgccgtaga tggggctgga gctatggtta taaggatgaa tcgagtcagc
     3061 cacgcttgaa tggaaaatag tcacccggtc gcgcgacgcg gcatcggcat tgcgcaaaaa
     3121 gtacggtccc agcgctgcgc cggctgccag cgtggcgctc tctttaagaa actgtcgcct
     3181 agtcactgcc gatcgtttct tcataattct ctcctatctt gattatctct gccgacgcag
     3241 cttgggatcg aaaatatcgc gcaatccgtc gcccaacaga ttgataccga acacggtaat
     3301 gaaaatcgcc aatccgggga atatcgttaa ccagggtgcc cgattgatgt agcttcggcc
     3361 ctcagccagc atcgaacccc aggttggaat atggaccggc acgcccaggc caaggaaact
     3421 cagactcgct tcggtgataa tgacaatcgc catgccaaac gtcgcgatta cggtaacact
     3481 cgcgacggta ttgggaatca cgtggcggaa aatcacgtaa gcgtctttgg caccgagcgc
     3541 cttggctgcg gtgatgaaat cgcgctcttt caaggacagc acttcgccgc gcacgactcg
     3601 gcaatactgc acccaccgac ttagcattaa tgccatgatc agattaccca caccttgacc
     3661 gagaaaagcc atgatggcga tggcgatcaa cagaaatggg aaagccagga atatctcgga
     3721 tattttgctg acgatctcat cggcggtgct gccgaagtag ccggcgatgg cgccgagggt
     3781 gcagccgacg aggccggcaa aaatcaccgt ggctgcgcca accatcaaag aaatgcgcga
     3841 accgtaaaca atccggctga gcatgtcgcg gcccaaatta tcggtgccaa gaaaataagc
     3901 ctgcatacca ttgtccgtcc agcgcggcgg ccgcaaccgg tcctccaaag tttgcgcttg
     3961 cggatcgcgc ggcgcgatca acggtgcggc gacgccgcag acgacgagaa gcgctaatat
     4021 tgcaccgccg acccaaagtt tcagatagcg gatagacaac atcagaactt gatcctcgga
     4081 tcgatccaac tgtagagaaa gtccaccaac aaattgacca cgaaataggt caccgcgaac
     4141 atcaaaatca acacttgggt gagtttgtag tcgcgcgcgg aaatcgactg gatcaggaga
     4201 tcgccgatgc cggggtagga aaaaaccgtt tccgtcacca cagagccgct gagcaaggcg
     4261 cccaattgca gcccaagaat cgtcacgatg gtgatcaagg cattgcgcag gatgtgccgc
     4321 cagatcacgg ttttttcgct caaccccttg gcgcgagccg tagtcacata atcctggcgc
     4381 atcacttcga gcacactcga gcgcgagatg cgcgtgacga tggccgccag agcggcgccg
     4441 agggtaaatg ccggcatcaa caaatgcttc gcgacgctga caaacccgtc aaagtttccg
     4501 gtaattatac aatcgacaag atacaggccg gtaatcgact tgatttcaat gccatactcg
     4561 acccgaccag ctaccggcaa aagattgagc tggccgccga gaaaatagat cagcatgatg
     4621 cccagccaaa aattcggcag cgaaacgccg atcagtgcga cgctcattcc cgcgtgatcg
     4681 agcagtgaat tatggcgtat cgccgaatag acaccgaccg gcacggcgat cgcaatagcg
     4741 atgaacaggc tggcgaaagc gagttcgaga gtcgccggca gccgctcggc gataagcttg
     4801 attaccggct cgccgaactt gagagagcgg ccgaagtcgc cgcgcacggc acgcgagagg
     4861 aaatcccaat actggaccag caatggtttg tcgaatccga gttcggcacg cagtttcgcg
     4921 acgtcttccg gtgtcgcgtt gtcaccgagc atcaaggtga ccggatctcc cggcgcaata
     4981 tgcaccaaaa gaaaaattaa aaacgaaatc gctaataaga ccggcagggc tccgataaat
     5041 ctacgaatga gcttgttcat ggacgcagct aaaaaacgct ttacctaaca ttccgctttc
     5101 tggttggaga ggaatatcac tactgcccta gctgtccaaa cgattggtcc aaaactttca
     5161 acgcatggtc gacttcgtcc ttattgacat tcaaagacgg cgcgacgcgg atcacgttgc
     5221 cataggtgcc gcctttgccg agcaaaagac cgttgttctt ggtcaactcc atcagccgtt
     5281 tggtgctttg ggcgtcgggc ttcttttgtg cgccgaccag ttccataccc tgcattaagc
     5341 ccatgccgcg aacgtccccg accaatggat atttgctctt caaggcgtcc aatccgtcac
     5401 gtagacgatt gcccatgacg cgagcgtttt cgacgaggtt ctcttcttcg atgacgttga
     5461 tcgtcgcgtg cgccgccgtg caggttacag gattgccgcc aaacgtcgag atcgtattgc
     5521 cctgcatgct gtcggcgacc tccgctgtcg caatggtggc gccgatggga acgccattgg
     5581 ccataccttt ggcgaacgtc atgatgtcag gttcgacgcc ccaatgctcg atgccgaaca
     5641 tcttgccacc ggtacgcccc cagccggtct gcacctcgtc ggcaatgaac aaaccgccat
     5701 attttcgcac gatgccgacg atctctttga agtattcttt cggcggcacg atgaaaccac
     5761 cgactccttg aatcggttcg gcgaggaagg ccgcgactcg tccctgcgaa gtcatggttt
     5821 gaatcgcttc ttcggcatct ttggcgcact tcaaatcgca gctcgggtag gtgagaccga
     5881 aggggcagcg ataacaatag gcgttggcga tatgatggac gccgggcacc acattgggca
     5941 tcagacgcca gctcgactgc gccgataggc tgagcgccaa gtaggaacgg ccgctgtagc
     6001 tatggcgcag ggtaatgacg tcctggcatt tcgtatagat ctgcgccaac agcaccgcag
     6061 tctcgttggc ctcggtgccg ctgttggtga aaaatgattt ttccagtcgg cccggcgtga
     6121 tttgcgccag cttctcggcc aagttgacct gcggctcgtt ggcgtagagc gtcgacatat
     6181 gctgaaactt acgattttgc tcgtcgatcg ccttggtaac tttttcattg acatgtccga
     6241 cactgacggt aagtatgccg ccgaagaaat ccagatactc cttgccgtcg gcgtcgaata
     6301 ccgaatgatc cttggcatga tcgatgacca agggctcgtc gtaataagta gcaacgcaag
     6361 agaacaaata ttttttctgc ttttcgagaa c
//
LOCUS       DATLCH010000058         3504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5411445, whole genome shotgun sequence.
ACCESSION   DATLCH010000058 DATLCH010000000
VERSION     DATLCH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5411445"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VMZ02_01625"
     CDS             complement(<1..1066)
                     /locus_tag="VMZ02_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70654.1"
                     /translation="MQIIDADGHVNDRPCMDEISKFMPAGNRTQVFPAFDHIHFHYLE
                     GGEKRSRTGDVGPKEWLDFLDETEIDWTVVYPTAGLAVGRIIAEDWAVAACRAYNNWL
                     YEKFTKVTPRIKGMALIPIQDSQSACEELHRAVKELGMAGAMLPSNGEGIKGHYGSKI
                     YWPIYEEAEKLNVPLAVHVGALHHLGMDSIGVYYPVHALGHPFGIMAQAAAMLSYGIF
                     DRFPKLRVGFLEGGATWVPFFMDRLDRSYPHHLQVDIHGEFLPSSQPDVMASEYFRRH
                     AKAGRIFVGFDGDDHGLGYAIKEAGNESFVFATDFPHESFDAKSCRIELQHILSREDL
                     SQADKEAILGKNAKRFYGVER"
     gene            1221..2222
                     /locus_tag="VMZ02_01630"
     CDS             1221..2222
                     /locus_tag="VMZ02_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70655.1"
                     /translation="MKIVLTTLTLIVALARACFAQDAKTIDAAKKEGGKVVIYGSLES
                     QVVDEVLAGFRKKTGLTAEYWRASAMSVMNRSMSEYRAGAPLFDIVMNNSDPLVIMAN
                     EGMIAKYDSPTAKKYPKDQIDPRLGPVSRYGIVGVVFHKGLIKAEDAPKSLEELVSAK
                     YKGMLVMADPTLHVTTIQWLSSLHKIMPKDRADKFIRDLAAMKPTLVESMLPAAERMA
                     NGEFPIALTFVKYAYSAAKGGAPLDYMRVDKMLGDAHFAVLSAKAARPNAGKAFIDYY
                     LDDDNMKVLAQAGEFPNRKGIYPPLPGADKIQYVQMEVLEAKAFEEKKKEFAKIFLK"
     gene            complement(2420..2944)
                     /locus_tag="VMZ02_01635"
     CDS             complement(2420..2944)
                     /locus_tag="VMZ02_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HUR70656.1"
                     /translation="MSIYQLGDDKPIIPASAYVAPEATLIGKVILGENASVWPGVVIR
                     GDNDSITIGSGSNVQDGSVLHTDPGFPLTVGDDVTIGHQAMLHGCTIGDGCLIGIQTL
                     VMNSAVIGNDCLVGAGALVPEGKSYGERKLIVGSPARVIRELTDENIEKMHRAAEGYV
                     RRKDMYRLNLKRIG"
     gene            complement(3120..>3504)
                     /locus_tag="VMZ02_01640"
     CDS             complement(3120..>3504)
                     /locus_tag="VMZ02_01640"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70657.1"
                     /translation="VAVKQRYPGHVKQAGMIASQCFSGGYQNRFTIVVDDDIDPTRID
                     DVVWALSTRCDPADDIDIQRRSWSSALDPMIHPDSKVFLNSRAIVDACRPYEWMSKFP
                     EVAETSQELRKKVLTKLGRNFFGLR"
BASE COUNT          763 a          934 c         1010 g          797 t
ORIGIN      
        1 agcgctcgac gccgtaaaat ctttttgcgt tctttcccag gatcgcttct ttgtcagcct
       61 gactgagatc ttcgcggctg agaatatgct gcagctcgat acgacaggat ttagcgtcga
      121 aactctcgtg cggaaaatcg gtggcaaaga caaatgattc gttcccggct tccttgatgg
      181 cgtagccgag gccgtgatca tcgccgtcga agccgacaaa aatcctgccg gccttggcgt
      241 gacggcgaaa gtattcgctg gccatcacat cgggctgcga gctcggcaag aattcgccat
      301 ggatatcgac ctgtaagtga tgcgggtagg agcgatcgag acgatccatg aagaacggta
      361 cccaggttgc gccgccttcg agaaatccga cgcgcaactt ggggaatcgg tcgaagatgc
      421 cgtaggacag catggcggcc gcttgtgcca tgatgccgaa cggatggccg agagcgtgca
      481 ccggatagta aacaccgatg ctgtccatac ccaaatggtg cagcgcgccg acgtgaaccg
      541 cgagcggtac attcagcttc tcggcttctt cgtaaatcgg ccaataaatt ttggagccgt
      601 agtgcccttt gataccctcg ccgttggacg gcagcatggc gcctgccata ccgagttctt
      661 tgaccgcgcg gtgcaattct tcacaggccg attggctatc ctggatcggg atcagcgcca
      721 tacctttgat gcgcggtgtg accttggtaa atttttcgta gagccaattg ttgtaagcgc
      781 ggcaggcggc gacagcccaa tcttccgcga tgatcctgcc gacggctaaa cctgcggtcg
      841 ggtagacgac ggtccagtcg atttcggttt cgtcgagaaa gtcgagccac tccttgggac
      901 cgacgtcgcc ggtgcgcgag cgcttttcgc caccttccaa gtaatgaaag tggatgtgat
      961 cgaaggcggg aaatacctgg gtgcgattgc ccgccggcat gaatttggaa atttcatcca
     1021 tgcaggggcg atcgttgacg tggccgtcgg cgtcgatgat ttgcatttgc tcgcttacct
     1081 ttccacgaaa ctctatgggc tataacaagt tggctttggc agtgtcaacg cgactggttc
     1141 aagagttcac aagttgccgg gttcaacgtt gaccggcgaa ttccagccgt gatagttgga
     1201 gtctctgtta ggaggatcag atgaaaattg ttttgacgac tttgacactc atcgtggctc
     1261 tggctcgagc ctgtttcgcc caggatgcga aaacgatcga cgccgccaag aaagaaggcg
     1321 gcaaggtggt tatttatggc tcgctggaaa gtcaggtggt ggatgaagtg ctcgcgggtt
     1381 ttcgcaagaa gaccgggctc acggccgagt actggcgcgc gtcggcgatg agcgtgatga
     1441 accgctcgat gagcgagtat cgcgccggcg ctccgctctt cgacatcgtc atgaacaatt
     1501 ccgacccgct ggtgatcatg gccaacgaag gcatgatcgc caagtacgat tcgccgacgg
     1561 cgaagaaata ccccaaggac cagatcgatc cgcgtctcgg cccggtgagc cgctacggca
     1621 tcgtcggcgt ggtgtttcac aagggcttga tcaaagccga ggacgcgccg aagtcgctcg
     1681 aggaactggt cagcgcaaaa tacaaaggca tgctggtgat ggccgatccg acgttgcacg
     1741 taaccactat tcaatggctg tcgagcctgc ataagatcat gcccaaagat cgcgccgata
     1801 aattcatccg cgatttggcg gcaatgaagc cgaccctagt cgaatcgatg ctgccggcgg
     1861 ctgaacgcat ggccaacggc gaatttccca tcgccctgac attcgtcaag tacgcctatt
     1921 cggcggcaaa aggcggcgcg ccgctggatt acatgcgtgt cgacaaaatg ctcggcgacg
     1981 cccatttcgc tgtgctcagt gccaaggcgg cgcgtcccaa tgccggcaaa gcttttatcg
     2041 attactatct cgacgacgac aatatgaaag ttctcgccca agccggcgag ttccccaacc
     2101 gcaaagggat ctacccgccg ctcccgggcg cggataagat tcaatatgta cagatggaag
     2161 tgctggaagc gaaagcgttc gaggaaaaaa agaaggagtt tgccaagatt tttctaaaat
     2221 gattacgaac ggtaaatcct acttggatga ttaacgccca agtgctggtg ccggtgcaaa
     2281 ccgcaatcct ttttaatttt gacttgcggg cagtcaacct ggattccggc gtgcgccgga
     2341 atgacggaac gggacgtgga atgcttagag cccgctcggt ttcggaggcg gaatactttt
     2401 ctgtgacttg ctgattcaac taaccgattc gcttcaagtt caatctgtac atatccttcc
     2461 gccgcacata gccttctgcg gcgcggtgca ttttttcgat attttcatcc gtcagttcgc
     2521 ggataactct tgcgggagag ccaacgataa gtttgcgttc gccgtaactt ttaccttccg
     2581 gcaccaaggc gccggctccc acgagacagt cgttgccgat gacggcgctg ttcatcacta
     2641 acgtttgaat gccaatgagg cagccgtcgc cgatggtgca accgtgcagc atagcttgat
     2701 ggccaatggt gacatcgtcg ccgaccgtga ggggaaatcc cggatcggtg tgcagcacgc
     2761 ttccgtcttg gacgttgctg ccgctgccga tcgtaatcga atcgttgtcg ccgcggatca
     2821 ctacgccggg ccagacgctg gcgttttcac ccaagatcac tttgccgatg agcgtggctt
     2881 caggagcgac gtaggctgaa gcggggatga tcggtttatc gtcgccgagt tggtagatag
     2941 acattagttt gctcccataa atttgttcaa aacgttcaag ccgtcaaccg cttcgctccg
     3001 ttcaagcccc gacctttctg cgatatgatc acgtgggcta tcctcccccg cgtcgcgggg
     3061 gaggatttag gagggggttt gagcgatttg aacggcttca acggtttgaa cgagtggttt
     3121 catcgaagtc caaaaaaatt gcggcctagt ttagtcaata ctttctttcg tagctcctga
     3181 ctcgtctctg ccacttctgg aaatttgctc atccattcat aaggccgaca ggcgtcgacg
     3241 atggcgcggg aattcaaaaa tactttcgaa tccgggtgaa tcatcgggtc caaagcgctt
     3301 gaccagctgc gccgctggat gtcgatgtcg tcggccggat cgcagcgcgt cgatagcgcc
     3361 cagacgacgt cgtcgattct tgtcgggtcg atgtcatcgt cgacaacgat ggtgaagcgg
     3421 ttctgatagc cgccggagaa gcactgcgac gcgatcatgc cggcttgctt aacgtgtccg
     3481 ggatagcgct gcttgacggc gacg
//
LOCUS       DATLCH010000059         6356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4660180, whole genome shotgun sequence.
ACCESSION   DATLCH010000059 DATLCH010000000
VERSION     DATLCH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4660180"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VMZ02_01645"
     CDS             <1..289
                     /locus_tag="VMZ02_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70658.1"
                     /translation="PEEYAKMGVAREQVLKLKTAFESGATVEQAATLVNDHMVRCYYA
                     AGKPEEVRDQIIDLVGAAGRLGYDHVAFAKLGPDYDEAIKLLSEQVVPGLR"
     gene            complement(339..1055)
                     /locus_tag="VMZ02_01650"
     CDS             complement(339..1055)
                     /locus_tag="VMZ02_01650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR70659.1"
                     /translation="MIKNRSHLFMFGLVGIFLAGCGATSTPNYSEVLTNTNRPEAERN
                     IDAVRKPNELMAFYGVRRGQKVADIFASRGYYTAILSQLVGPEGVVYSANPAPRQELH
                     DRLKQPGMANVRVLDGPFDKLALPQDASLDFVILHLDYHEIAADVRRPMNARIFGALK
                     SGGSYGVIDHSAKEGAGDSEAKTLHRMDKLLVIKEATSVGFRLAEEGKMLSRAEDTRD
                     FSVVKIRDRSDRFVLKFVKP"
     gene            complement(1079..1801)
                     /locus_tag="VMZ02_01655"
     CDS             complement(1079..1801)
                     /locus_tag="VMZ02_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70660.1"
                     /translation="MNHYVARSLRSLAVFTAMALMAACSATTPDYRRVLSNPDRPATE
                     RELDEARKPQEVLAFYGVKSGDKVADLWAGRGYYTAILSEVVGPTGIVYTQNPTARDE
                     INQRWKTGKFANVRVTDGTFDKLTLPQDGSLDFVLIHLNYHDVDPSLRSALNKAVLGA
                     LKRGGIYGVVDHSAKEGSGNDAVKTLHRIDKLQVIKEVTDAGFKLAKDGDMLRKPEDK
                     RDFNVNKERNKDDRFVLAFQRP"
     gene            complement(2203..2979)
                     /locus_tag="VMZ02_01660"
     CDS             complement(2203..2979)
                     /locus_tag="VMZ02_01660"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70661.1"
                     /translation="MKVVVNGVTKEFVTTQGTTRAIGGVNLEVRDNEFFGIIGPTGCG
                     KTTLLHIIAGLEKPSTGSVQFVGTQRGKSPVSMVFQESALMPWRNVEENVPLGAEFRH
                     EEQPVLKRVSRFFLEVVKLLDFAGARPHELSGGMKQKVAIARALANDPEVILMDEPFA
                     SLDAQTRLLMREELLRIWERDKKTVVLVTHNIDEAVMLCDRIAVMSARPGLIKSIVTV
                     DVPRPRTMKSMRDPDFAGCMDKIWNLMKYDVEHALQHGKP"
     gene            complement(3143..4561)
                     /locus_tag="VMZ02_01665"
     CDS             complement(3143..4561)
                     /locus_tag="VMZ02_01665"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR70662.1"
                     /translation="MPQDLRSYIDLIREKKLLLDIKKEVDPLTNLAGLAYRGENEQGK
                     ATMFHNLKGYANWTAVSYMCGSREKMALGLGTTKDKALTELGGRIAKGLIKPKIVEDG
                     PVKEIIWKGDDADLGRIPIHVHSDSDRGVPFIGSAMQFVKDPESGTQNIALQRDHIKG
                     PKKMGIFVHPKRHTDICMQKYWQEGKAMPVATVIGHHPAYYIASTWTTSYETDEIDIT
                     GALLQEPVNMVRCETIDVMVPADAEIVIEGEVPPNYMEDEGPFCEHAGMTHGTLQQPI
                     INVKCITMRKNPIYYALQGGRPISESQPLDGFPMELVLYARIKDVGGFIDVKDVVSLP
                     YAGGSHIIVVQMKPRKMGEVRSALMALLSSPYLHPKIGIAVDDDIDPHNAQSIMYAMS
                     TRVNPKSDVLIIPDTLCHTGDLTAEIVPVSGAHMGFHPRIGSKMLIDATKGPVLTDEL
                     RNYFEPVKPQGLANVRLEDFLG"
     gene            complement(4835..5860)
                     /locus_tag="VMZ02_01670"
     CDS             complement(4835..5860)
                     /locus_tag="VMZ02_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70663.1"
                     /translation="MFRSKFKVQRSRFVKCAAVAALVFPFTGAAWAQDVKSLAEAEGK
                     MVFYAAFNATDTKTLTDGFQKLYPKIEASFYRATDAQLMERILTEYRAGKPIADVVMS
                     TSFYGHNLKKRGMLAAYDSPERKFFRDGYKDPQGTWTSIYTNYSAFGFNTRNVPRASV
                     PKTHNDLLKPEWKGNIGIDTRAYEWFGTMVKAMGEEKGLAFMRELAKQVGLRNGRTIL
                     AQLVAAGEFKGALSAYSQTFEQMKPAGAPVDWVYLNPVFANIHPVGVSAKAPHPNAAK
                     LFIDFVLSKRGQELVRGMNRIPDRSDTPPEQARFIEGIKPVFAPVEVLENFEGYAKTF
                     HEVFGGK"
     gene            complement(5860..>6356)
                     /locus_tag="VMZ02_01675"
     CDS             complement(5860..>6356)
                     /locus_tag="VMZ02_01675"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70664.1"
                     /translation="PPTMVRGHGADRQIADSLTAKYKIPVTVATIAQVDAFRALGMKK
                     LVGITYFQDDMNQRFAKFLEDDGFEVKAMKGIEVPFKDAGKLAPEAIYNFAKKIFLAS
                     GKADGIYMLGAGWHNLPVIEMLEKDLKTTVLSSIPAQVWATKKILNLNEPLQGYGRLL
                     AEMP"
BASE COUNT         1347 a         1791 c         1723 g         1495 t
ORIGIN      
        1 accggaagaa tacgccaaga tgggtgtcgc ccgtgagcaa gtgctaaaac taaaaaccgc
       61 attcgagtcc ggcgccaccg tcgaacaagc cgcgacactg gtaaacgatc acatggtgcg
      121 ctgctactac gccgcaggaa aaccggaaga ggtgagagat cagattatcg atctggtcgg
      181 cgcggccggc aggttaggct acgatcatgt ggccttcgcc aaacttggcc cggattatga
      241 tgaagcaatc aaactgctga gcgagcaagt ggtaccgggc cttcggtaaa cgcccggacg
      301 attatcctgc gcggaaccgt cagcgaatcc gacgatgctt acggcttgac aaacttcagg
      361 acaaatcgat cgctcctatc gcgaatcttg accacactga aatcgcgggt atcttctgcg
      421 cggctgagca tttttccttc ttcggctaaa cgaaaaccga cgctggttgc ttccttaatc
      481 accaggagtt tgtccatgcg gtgcagcgtc ttggcttcgc tatcgccggc gccctctttc
      541 gcggaatggt cgatgacgcc gtagctgccg ccgctcttta gtgcgccgaa gatccgcgcg
      601 ttcattgggc gccggacatc ggcggcgatc tcgtgatagt cgagatggag aataacaaaa
      661 tccagtgaag cgtcctgcgg cagcgccagc ttgtcgaacg gtccatcgag cactcgcaca
      721 ttggccatgc cgggctgttt cagcctgtcg tgcaattcct ggcgcggcgc tgggttggcg
      781 gaatagacca ctccttcggg tccgaccagc tgggatagaa tggccgtgta gtaacctcgg
      841 ctagcgaaaa tatcggcgac tttttggccg cgtctgacgc catagaaggc catcagctca
      901 ttgggcttgc ggactgcatc gatgtttcgt tccgcttccg ggcggttggt gttcgtcaaa
      961 acttcggaat aatttggcgt cgaagtcgcg ccgcagcccg ccaggaaaat tcccaccaat
     1021 ccgaacatga aaaggtgtga ccggttcttg atcataaatt actccgagtt tgtctcgttt
     1081 acggacgttg gaatgccagc acgaagcggt cgtctttatt gcgctccttg ttgacattga
     1141 agtcgcgttt atcctcaggc ttgcgcagca tgtcaccgtc ctttgccaat ttaaaacccg
     1201 catcggtcac ttctttaatg acctgcagtt tatcgatgcg gtggagcgtt ttcactgcgt
     1261 cgttgccgga gccctctttc gctgaatgat cgacgacgcc gtagatgccg ccgcgtttga
     1321 gcgcgccgag aaccgccttg ttcaatgcgc tgcgcagact agggtcgaca tcgtgataat
     1381 tgaggtggat cagcacaaaa tccaacgaac cgtcctgtgg caacgtcagc ttgtcgaacg
     1441 tgccgtccgt gacgcgcacg ttggcaaatt tcccagtctt ccagcgctgg ttgatttcat
     1501 ccctcgccgt aggattctgc gtgtagacta tgccggttgg gccgacgact tcggataaga
     1561 tcgccgtata atagccgcgc ccggcccata gatcggcaac tttatcgccg ctctttacgc
     1621 cgtaaaacgc caaaacctct tgaggcttgc gtgcctcatc gagttcgcgc tcagtggccg
     1681 gacgatcggg gttgctcaac acacggcgat aatccggcgt tgtcgcggag catgcggcca
     1741 tcagcgccat tgcggtaaac acggctagtg atcgcaaaga tcttgcaacg tagtgattca
     1801 ttagaattcc ttccgcagga gagagttagg tccaactgta gtgaatgaac aaacaaaagc
     1861 aagcaaaaag ggctgggtta aatccaagct atttccgcgt tccgtgagga ttttattttg
     1921 tgacctttgt gctccctaga aaatactatg caggtacgat ctggccgacg gattggcaga
     1981 agggcttccg ttatcccggc gaaagccggt atcgatccac tcccgcccct ggatgccggc
     2041 ctgcgccggc atgacgaatc aagggagaaa ccatgtggtc ctgggaggac ggcaatttta
     2101 tactctgtag acgagcgtaa gctcatgaac agtttggtta agactccgat gcggaatatt
     2161 cgctccgctc agtgctacgt cgcacgactg ttgtaaactc cttcacggct tcccgtgttg
     2221 caacgcgtgt tcgacgtcgt acttcatcag attccagatc ttgtccatgc agccggcgaa
     2281 gtcaggatcg cgcatgcttt tcatcgttcg cggccgcggc acatcgacgg tgacgatgct
     2341 cttgatcaag cccggccgtg cgctcatgac cgcgatgcgg tcgcacagca tcaccgcttc
     2401 gtcgatgttg tgggtgacga ggacgacggt ctttttgtcg cgctcccaga tgcgcaaaag
     2461 ctcttcgcgc atgagcaaac gggtttgcgc gtccaagctg gcgaagggct cgtccattag
     2521 aatcacttcg gggtcatttg cgagggcgcg ggcgatggca actttctgct tcatgccgcc
     2581 ggataattca tgcggccggg ctccggcgaa atccaacagc ttgaccactt cgagaaaaaa
     2641 ccggctcact cgtttaagca ccggctgttc ctcatggcga aactcggcac cgagcggcac
     2701 gttttcttcg acattgcgcc aaggcatcag cgccgattcc tgaaacacca tcgataccgg
     2761 cgattttccc cgttgtgtgc cgacgaactg caccgaaccg gtgctgggct tttccaaacc
     2821 ggcgatgatg tgcaaaagcg tcgtcttgcc gcagccggtc ggcccgataa tcccgaagaa
     2881 ctcattatcg cgcacctcga ggttgacccc gccgatggcc cgcgtcgtcc cctgggtggt
     2941 gacaaattct ttggtaaccc cgttaacgac gactttcata ctaacaagct cggaaattta
     3001 gccgcaaaga acgcaaagta cacaaaattc agaaatttaa acattcactg gactgctcga
     3061 caatcgtgca attccatgaa gtcatttcga gcaaagcgag aaatctaatt tgcgaccttt
     3121 gcgttctttg cggccattcc tcttatccga gaaaatcctc cagccgcaca ttcgccaatc
     3181 cctgcggttt caccggttcg aagtagtttc tcagctcgtc cgtcagcacc ggccctttgg
     3241 tggcgtcgat cagcatcttc gacccgatgc gcggatgaaa tcccatatgc gcgccgctga
     3301 ccggtactat ttcagcggta aggtcgccgg tatgacacag cgtgtcggga ataataagca
     3361 cgtcggattt gggatttacc cgggtagaca tggcatacat aatcgattgc gcattgtgtg
     3421 ggtcgatgtc gtcgtcgacg gctatgccga tcttgggatg gagatacggg ctcgacaaca
     3481 gcgccatcag cgcgctgcgc acttcgccca ttttgcgcgg cttcatctgc acgacaataa
     3541 tatggctgcc gccggcatag ggcaaagaga caacatcttt aacgtcgata aagccgccga
     3601 cgtctttgat gcgcgcgtat aaaaccagct ccattggaaa tccatccaac ggctgcgact
     3661 cggaaatcgg ccggccgccc tggagtgcgt agtagatcgg gttcttgcgc atggtgatgc
     3721 atttgacgtt gatgatcggc tgctgcagtg tgccgtgggt catgccggcg tgctcgcaga
     3781 acgggccttc gtcttccatg tagttgggcg gaacttcgcc ctcgatgacg atctcggcat
     3841 ccgccggcac catgacgtcg atggtttcgc aacgcaccat gttgacgggc tcttgcagta
     3901 gcgcgccggt gatatcgatc tcatcggttt cataactggt cgtccaggtc gatgcgatgt
     3961 agtaagccgg atgatggccg atcaccgtcg ccaccggcat cgctttgccc tcttgccagt
     4021 atttctgcat gcagatatcg gtatggcgct tgggatgaac gaagatgccc atctttttag
     4081 gacctttgat atggtcgcgc tgtaaggcga tattctgcgt gccgctttcc ggatctttga
     4141 cgaactgcat cgccgaaccg ataaacggca cgccgcggtc gctgtcggag tgcacgtgaa
     4201 tcggaatgcg cccgagatcg gcgtcgtcgc ctttccagat gatctccttt accggaccgt
     4261 cttcgacgat cttcggtttg atcaggcctt tggcgatccg cccgccgagt tcggttaacg
     4321 ctttgtcttt ggtagtgccc aaccccaacg ccatcttctc ccggctgccg cacatgtaac
     4381 tcaccgccgt ccaattcgcg tagcctttga gattgtgaaa catcgtcgcc ttgccctgct
     4441 cgttctcgcc gcgataggca agaccggcca agttggtcaa aggatcgact tcctttttga
     4501 tgtcgagcaa aagcttcttt tcacgaatca gatcgatgta gctacgcaga tcctgtggca
     4561 taggaaactc atccctccaa aaatattctt ggaatgttgt attgcagatc gttcgcggaa
     4621 tgcaacgcct ttccgcacgg gccgatggtt tagagactgt gtcgtaattg tcatttcgag
     4681 ccgaaggcga gaaatcttgc gtttatatgt ggttggaaat gcaagatctc tcgctgacgc
     4741 tcgagatgac aatgtgcacg attccagaat tgcgacacag tctctccggg ggaaggcatc
     4801 aggacaccca acacagaacc gtgcgcgtcg attgctattt gccgccaaag acctcgtgaa
     4861 aagtcttggc atagccttcg aagttttcca gcacttcgac cggcgcgaag accggtttga
     4921 tgccttcgat gaagcgcgct tgctccggag gagtgtcgct gcggtcgggg atacgattca
     4981 tgccgcgcac caactcctgg ccgcgcttcg atagcacgaa gtcgataaac agtttggcgg
     5041 cgttgggatg cggcgctttg gccgagacgc cgacggggtg aatgttggcg aacacgggat
     5101 ttagatagac ccaatcgacc ggcgcgccgg ccggcttcat ctgctcgaaa gtttgcgagt
     5161 acgcgctgag cgcccctttg aattcaccgg cggcaaccag ttgcgccagg atcgttcgac
     5221 cgttacgcag cccgacctgt ttggccagtt cgcgcatgaa agcgagcccc ttctcttcgc
     5281 ccatcgcttt gaccatcgtg ccaaaccact cataggcgcg cgtatcgatg ccgatattgc
     5341 ccttccactc cggtttcagc agatcgttgt gggttttggg aacgctcgca cgcggcacat
     5401 tgcgcgtgtt gaaaccgaac gccgaatagt tggtataaat ggatgtccaa gttccttgcg
     5461 ggtccttgta accgtcgcga aagaatttac gctcgggtga atcgtaagcc gccagcatgc
     5521 cgcgcttctt gagattgtgg ccgtagaaac tcgtgctcat taccacgtcg gcgattggtt
     5581 tgccggcgcg gtattccgtc agaatgcgtt ccatcaactg cgcgtcggtg gcgcgataaa
     5641 aactcgcttc gatttttgga taaagtttct gaaagccatc ggtcaaagtc ttggtatccg
     5701 tggcgttgaa ggcggcgtaa aaaaccatct tgccttctgc ctcggcaagc gacttcacat
     5761 cctgagccca agccgcaccg gtgaatggaa acaccaacgc cgcgacagcc gcgcacttca
     5821 caaaccttga acgttgaacc ttgaactttg aacgaaacat cacggcatct ccgccaaaag
     5881 ccgcccatag ccctgcagcg gttcgttgag attaagaatt ttcttagtcg cccatacctg
     5941 cgccggaata ctcgacagca cggtggtctt caagtctttt tccaacatct cgatcaccgg
     6001 cagattgtgc caacccgcgc cgagcatata aataccgtcg gctttgccgc tggcgagaaa
     6061 aatcttcttg gcgaagttgt agatcgcctc aggcgccagc ttgccggcgt ctttgaacgg
     6121 cacttcgatg cccttcattg ccttcacctc gaagccgtcg tcctcaagaa actttgcgaa
     6181 ccgctggttc atatcgtctt gaaaataggt gatgccaacc agcttcttca tgccgagcgc
     6241 gcgaaacgcg tcaacttgcg cgatggtcgc gacggtgacc ggtattttgt atttcgccgt
     6301 cagtgaatcg gcaatctgcc ggtccgcgcc atgcccgcgc accatggtcg gtggcg
//
LOCUS       DATLCH010000060        11477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_196088, whole genome shotgun sequence.
ACCESSION   DATLCH010000060 DATLCH010000000
VERSION     DATLCH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196088"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VMZ02_01680"
     CDS             <1..262
                     /locus_tag="VMZ02_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HUR70665.1"
                     /translation="HAVFEATHGTAPKYADLDKVNPGSVILSGEMMLRYMGWNEAADL
                     IIKGIEGAVNNKTVTYDFERLMPGSKLVSCSGFGDAIVANMK"
     gene            307..1251
                     /gene="mdh"
                     /locus_tag="VMZ02_01685"
     CDS             307..1251
                     /gene="mdh"
                     /locus_tag="VMZ02_01685"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HUR70666.1"
                     /translation="MARKKIALIGAGNIGGSMAHLALLKGLGDVVLFDVVDGLPQGKA
                     LDLSHAGPVEGFDGNVVGTNKYDDIAGADVCIVTAGIARKPGMSRDDLLGTNAKILSS
                     VADGIKTHAPSSFVIVITNPLDAMVTLMQRKLGFPKHRVVGQSGILDSSRYRSFIAKE
                     LNVSVKSVHAMVLGGHGDDMVPVRSSCIIGGVPVEKLISGKRLDEIEARVRNAGGEVV
                     ALLKTGSAYFSPASAAIQMTEAFLFDKKEILPCAAMLEGEYGVNGYYFCVPVQIGAGG
                     VERIIDIGLSASDKKAFDESLNHVKDIVQAMDRVLSAA"
     gene            1327..2487
                     /gene="sucC"
                     /locus_tag="VMZ02_01690"
     CDS             1327..2487
                     /gene="sucC"
                     /locus_tag="VMZ02_01690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HUR70667.1"
                     /translation="MNIHEFQAKEILKRFGVMVPRSIVAATADEARKAAQTLGPGISV
                     VKAQIHAGGRGKGGGVKVVKSPDEAAARAGEMLGKNLVTHQTGPEGRRVRRVLVEQGV
                     NIAREIYLAIVLDRAQSRVTVICSSEGGVEIEEVAEKHPEKLLKETVDPVIGLAAFQC
                     RRVAFALGIPPQVVGKFVAVMQGLYRAFDECDAALAEINPLILTKEGEVMALDAKMNF
                     DSNAFFRQKDIVALRDTDEEDPREVEASKFDLSYISLDGNIACMVNGAGLAMATMDII
                     KFYGGEPANFLDVGGGADKAKVANAFRILLADDRVKGVLINIFGGIMRCDILAQGVVD
                     AARELSIKVPVVVRMQGTNVEQGRKILADSGLTIITAETMAEAAEKIVNAVK"
     gene            2565..3437
                     /gene="sucD"
                     /locus_tag="VMZ02_01695"
     CDS             2565..3437
                     /gene="sucD"
                     /locus_tag="VMZ02_01695"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HUR70668.1"
                     /translation="MAILVDKNTRVLTQGITGATGQFHTKACKEYGTQMVAGVTPGKG
                     GTAYEGIPIFDTVEQAVRETGANATVIYVPPPFAADAILEAADADIPLIICITEGVPV
                     RDMVKVKRFLVGRRSQLLGPNCPGTITPGECKIGIMPGHIHRPGSVGVVSRSGTLTYE
                     AVDQLTRLGMGQSTCVGIGGDPVHGFDFLDVLKLFNDDPGTEAVVMIGEIGGSSEEDA
                     AAWIKAHMKKPMAGFIAGQTAPTGKRMGHAGAIISGGKGTAKEKIAAMKQAGIRMASS
                     PADIGRSMQEAMGK"
     gene            3445..3858
                     /gene="ndk"
                     /locus_tag="VMZ02_01700"
     CDS             3445..3858
                     /gene="ndk"
                     /locus_tag="VMZ02_01700"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HUR70669.1"
                     /translation="MERTLSIVKPDAVKRNLIGAILKKFEAVGLRIAAGKLIRLTPDQ
                     AQSFYAVHKARPFFQGLCDYMSSGPIFVSVLEGDNAIARNREVMGATDPAKAATGTIR
                     QEFGEDIEKNAVHGSDAPETAATEIAFYFKPDEIY"
     gene            3860..4963
                     /gene="rlmN"
                     /locus_tag="VMZ02_01705"
     CDS             3860..4963
                     /gene="rlmN"
                     /locus_tag="VMZ02_01705"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HUR70670.1"
                     /translation="MSPYWVGNVKADGKPNIKDLSSGELGEFLAAAKQPGYRAKQIWQ
                     WLFREHVTEFASMTNLPVPLREQLACSFTIGRLAIERRHDSRDGTVKFLFGLADGKSI
                     ESVVIPERQRLTICISTQVGCAYGCAFCATGLLGFKRNLTCGEIVEQILEANRALVED
                     RRITNVVLMGMGEPLANYEQTRRALEIMIDPAWGLAISPRKITLSSVGLVPQIQKLME
                     ETNITLAISLHAATEELRGRLMPVNRKYSLEELIDCCRNLPLPRRKRITFEYVLLSGV
                     NDSLADAKALCQVLRGVPSKVNVIPFNPHPGSEFQRPSDDAVQRFQQVLFDHKIQVNV
                     RRPRGDDIAAACGQLQGQAQSESLDTDARVAAN"
     gene            4968..5828
                     /gene="mtnP"
                     /locus_tag="VMZ02_01710"
     CDS             4968..5828
                     /gene="mtnP"
                     /locus_tag="VMZ02_01710"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HUR70671.1"
                     /translation="MRHPTIGVIGGSGLYQMAGLKTVREVAVSTPFGKPSDKLIRGKL
                     GDSDVVFLPRHGKGHRWLPTEVNFRANIFAMKKLGVERIISISAVGSLREEIAPGHLV
                     VPDQFIDRTTQRPSTFFGKGLVAHVSMADPFCNDLSRVLQDSARQSGATVHRGGTYLC
                     MEGPQFSTRAESRLYRSWGAHVIGMTNLQEAKLAREAEICFATLALATDYDCWNEQAG
                     DVEIERVLTVLQNNVNLARRTIGAAVGLLNEPRSCSCASALKDAIITDRRAIPRKLRH
                     DLNPLIGKYL"
     gene            5825..6730
                     /locus_tag="VMZ02_01715"
     CDS             5825..6730
                     /locus_tag="VMZ02_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HUR70672.1"
                     /translation="MSVLVVGTVAFDSIETPSGSVERILGGSASYFALGASYFAPVRV
                     VGVIGQDFPQDHLDLFTQRGIDIEGIKREKGDTFHWRGRYHEDINQRDTIELHLNVLA
                     GFKPNLPERYRDTEYVFLGNIDPLIQMDVVEQIRHMKLLVCDTMDYWIRESNAALKKV
                     LQRIEMLVLNDSEARLLSGYDNIVKAARAILRMGPKMVLIKRGEYGVLQFSESAVFAT
                     PAYPLEEVFDPTGAGDSFAGGLMGQLARSGDASQGGMRRAIVYGSVVASFTVEDFGVK
                     RLAGLSLPDIEQRYQRFIELTDFHS"
     gene            6735..7688
                     /locus_tag="VMZ02_01720"
     CDS             6735..7688
                     /locus_tag="VMZ02_01720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUR70673.1"
                     /translation="MDLSVVIPVYNEEENVEPLITEIEAALAALGKKYEIIVVDDGSK
                     DSTFVKLRSIHKRVAKLQVVRLKRNFGQTAAIAAGLAHARGVVVILMDGDAQNDPADI
                     PALLRKLDEGNDLVAGWRYNRQDPFLNRRLPSMIANRLISSTTHVKLHDYGCTLKAMR
                     QDVAKNLRLYGEMHRFIPAIAYERGARIAELKVNHRPRLRGTSKYGITRTLRVALDLL
                     TVKFLSSYSTRPSHVFGPIGLGSGFLGFVIGLYLTAQKILYGQDIGGRPLLLLAVLLI
                     FIGFQFVTMGLLGEMLARTYHESQDRPVYVVGEVLGGGTGA"
     gene            7685..8989
                     /locus_tag="VMZ02_01725"
     CDS             7685..8989
                     /locus_tag="VMZ02_01725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HUR70674.1"
                     /translation="MKLAVVGTGYVGLVAGTCLSESGNDVICVDIDAQKIDLLKGGKV
                     PIYEPGLEEMVRRNAEDQRLSFTTDLPSAVKKSDVIFIAVGTPQGENGHANLEFVKST
                     AKSIAKALDSFKIIVTKSTVPVGTADQIKKWIAEETAQPFAVASNPEFMKEGAAVEDF
                     MKPDRVVLGGDDPAALATLRELYDPFVRTGNPILIMDSRSAEMSKYAANAMLATKISF
                     INEVARLCEQMNADIGEVRRAISLDRRIGPHFIFPGVGYGGSCFPKDTRAMIGMGGDH
                     QDMVLLRAVEQVNERQKQLLFDKVGRHFGGKLSGLTFAVWGLAFKPRTDDMRDAPAIN
                     IIESLLAAGARVQAFDPEAMEEARKIFGDRIQYTHRNYDALHGAAALLIVTEWNEFRR
                     PDFVRVKNLLTSPVIFDGRNIYDPADLRKLGFTYYSIGRNDG"
     gene            8982..9914
                     /locus_tag="VMZ02_01730"
     CDS             8982..9914
                     /locus_tag="VMZ02_01730"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HUR70675.1"
                     /translation="MARYLITGGAGFIGSHLCEYFLNQGHQVLCMDNYSTGANENIGS
                     LLKNPRFRFIDHNVSRYIEVPEPLDFVLHFASPASPVDYLELPIPTLKVGALGTHNTL
                     GLAKAKSATYLLASTSEVYGDPLVRPQSEEYWGNVNPIGPRGVYDEAKRFAEAITMAY
                     HRYHGLDSRIVRIFNTYGPRMRIRDGRVVPNFIMQALKDEPLTVYGQGQQTRSFQYVD
                     DLIAGLDKLLQSNHHLPVNIGNPYEMTVLQFANKIIEMTKSKSEIVYKPLPEDDPQVR
                     QPDITKARKILGWEPKVKLEEGLVKTIEYFRTKL"
     gene            9925..10851
                     /locus_tag="VMZ02_01735"
     CDS             9925..10851
                     /locus_tag="VMZ02_01735"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HUR70676.1"
                     /translation="MRILVTGGAGFIASHITDAYIERGHEVHIVDDFSTGQRANLNGK
                     ATLHQIDIADSHSVKLVERIKPDILSHHAAQMDVRHSVADPTFDARVNILGFINLLEG
                     CKNAGVKKVIFASSGGAVYGEQESFPASESHPTQPASPYGVSKRTGELYLSYYHQTFG
                     LPYLALRYANVYGPRQSTQGEAGVVAIFLALLLTGKTPVINGDGRQTRDYVYVGDVVA
                     ANVGALQSDFVGPVNIGTGVETDVVTIYDHLRVAVGSDMHAQHGPAKAGEQRRSCIDN
                     RRAGEVLGWRPQVALQDGLQHTAAFYREHLTQ"
     gene            10848..>11477
                     /locus_tag="VMZ02_01740"
     CDS             10848..>11477
                     /locus_tag="VMZ02_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HUR70677.1"
                     /translation="MKLDHIRNFSIIAHIDHGKSTLADRLLEFTGAVSEREKTDQILD
                     SMELERERGITIKASPVCLRYRAKDGKEYKFNLIDTPGHVDFTYEVSRSLAACEGALL
                     IVDAAQGVEAQTVANVHMALDLNLEVLPVINKIDLPSAEPERVRQEIEDVIGLDASNA
                     ILASAKEGVGIEDILEAVIHFFPPPQGDANAPLTASIIDCWFDPYHGAVV"
BASE COUNT         2563 a         3021 c         3368 g         2525 t
ORIGIN      
        1 tcacgcggta tttgaggcga ctcatggcac ggcgccgaag tatgccgatt tagataaagt
       61 gaatccgggg tcggtcattc tctcgggtga aatgatgcta cgttacatgg gctggaacga
      121 agcggccgac ttgatcatca agggcatcga gggcgcggtc aacaacaaaa ccgttaccta
      181 cgacttcgaa cggctcatgc ctgggtccaa gttggtgagc tgctccggct tcggcgacgc
      241 gatcgtggcc aacatgaagt aatcggcagt cgattaataa attttcatca taggaaagag
      301 cgaagtatgg ctaggaagaa aatcgcactg attggcgcgg gcaacatcgg tgggtcgatg
      361 gcgcatctgg cgctgcttaa aggcttgggc gatgtggtgt tgttcgacgt cgttgacggc
      421 ttgcctcaag gtaaagctct tgatctatcc cacgcagggc cggtcgaagg attcgatggc
      481 aatgtggtcg gcaccaacaa gtatgacgat attgccggcg ccgatgtttg tatcgtgacc
      541 gcgggtatcg cccggaagcc gggcatgagc cgggacgatt tgctgggtac caacgccaag
      601 attttgagct cggtggcgga cggaattaag acccatgcgc cgagttcgtt tgtcatcgtc
      661 attaccaatc cgctcgatgc catggtgact ttgatgcaac gcaagctcgg ttttccgaag
      721 caccgtgtgg tcggccaatc ggggattctc gattcctcgc gctaccgctc gtttatcgca
      781 aaggagctca atgtttcagt gaaaagcgtt catgcgatgg tattgggcgg gcacggcgac
      841 gatatggtgc cggtgcgcag ctcgtgtatc atcggcgggg tgccggtcga aaagttgatt
      901 tcgggcaaac gtctcgatga gatcgaggcg cgggtgcgta acgccggcgg cgaagtcgta
      961 gccctgttaa agaccggctc ggcttatttc tcaccggcct cggcggcgat tcaaatgacc
     1021 gaggcgtttc tcttcgacaa gaaggaaatc cttccgtgcg ccgcgatgct tgaaggcgaa
     1081 tatggcgtga atggctatta cttctgtgtg ccggtgcaga tcggcgccgg cggtgtcgaa
     1141 cggattatcg acattggtct aagtgcgtcg gacaagaagg ccttcgatga gtcactcaat
     1201 catgttaaag atatcgttca ggccatggac cgtgtcttga gcgcagctta agcagaatta
     1261 ggcacgagcg aaaccctgtg gccgatagta ggtcccaggg tttcatttta tcgggcatgt
     1321 gggcgcatga atattcacga atttcaggct aaggaaattc tcaagcgttt cggcgtgatg
     1381 gtgccgcgca gcatcgtggc tgccactgcg gatgaggcac ggaaggcggc acagactctg
     1441 ggccctggga tctctgtggt caaagcgcag atccatgccg ggggccgcgg caaaggcggg
     1501 ggagtcaagg tcgtcaaatc tcccgacgaa gcggctgcca gagcgggcga aatgctcggc
     1561 aagaatcttg tgacgcacca gactgggccc gaagggcggc gcgtccgccg cgttttggtc
     1621 gagcagggag tcaatattgc gcgcgaaatt tatcttgcca ttgtactcga tcgcgcgcag
     1681 tcccgtgtca cggtgatctg ttcctcggag ggtggcgtcg aaattgaaga ggtcgccgaa
     1741 aaacacccgg aaaagcttct caaagaaacg gtcgaccccg tgattgggtt ggcggcattt
     1801 caatgccggc gcgtcgcttt cgcgttggga attccgccac aagttgtcgg caagtttgtc
     1861 gccgttatgc aaggactcta tcgggcgttt gacgagtgcg atgcagcctt ggcagagatc
     1921 aatcctctga ttcttaccaa agaaggcgag gtcatggcgc ttgacgccaa gatgaatttc
     1981 gacagcaacg ccttttttcg ccaaaaagat atcgtcgctt tacgcgatac cgacgaagaa
     2041 gatccgcgcg aagtcgaggc cagcaagttc gatctctcgt atatttctct cgacggcaac
     2101 attgcttgca tggtcaacgg cgccggattg gcgatggcga ccatggacat cataaaattc
     2161 tatggtggcg agccggcgaa ctttctcgac gtcggcggcg gcgcggacaa agccaaggtt
     2221 gccaacgcat ttcgcatctt gctcgctgac gaccgggtca aaggtgtgct gatcaacatc
     2281 ttcggcggca tcatgcgctg cgatatactg gcgcagggag tcgtcgatgc cgcccgggaa
     2341 ttaagtatca aagttccggt ggtcgtgcgt atgcaaggga ccaatgtcga acaggggcga
     2401 aaaattctcg ccgattcggg tttaaccatt atcactgctg agacgatggc cgaagcagcc
     2461 gagaaaatcg ttaacgcggt caaataattg gtatctgaac tgccgaggaa atctcggata
     2521 agtacaggtc cagaaattcc acggcgaaaa cccgaccaaa ccttatggct attctagtag
     2581 ataagaatac gcgagttctg acccaaggca ttacgggtgc cactggacag tttcatacca
     2641 aagcgtgcaa agagtacggc actcagatgg tggctggagt gacgcccggc aaaggcggca
     2701 cggcctatga aggaattccc atcttcgata cggtcgaaca ggcggtgcgt gaaaccggcg
     2761 cgaatgcaac cgtgatttac gtccctccgc ctttcgcggc ggatgcgatt ctcgaagcgg
     2821 ccgatgccga tatccctttg atcatctgca tcaccgaggg tgtgccggtg cgcgatatgg
     2881 tcaaggtgaa acgatttctc gtcggacgaa ggtctcaact actcggcccc aattgtcccg
     2941 gaacgatcac ccccggcgaa tgtaagatag gcatcatgcc aggccacatc catcggccgg
     3001 ggagcgtcgg cgtcgtttca cgcagcggta cgttgaccta tgaagccgtc gatcagttga
     3061 cccgtttggg catgggacaa tcgacgtgtg tcggcatcgg cggcgatccg gtgcacggat
     3121 ttgatttcct cgatgtcttg aaactattca atgatgatcc cggcaccgaa gcggtggtga
     3181 tgatcggcga aatcggcggc agctccgaag aggacgcggc agcatggatt aaggcgcaca
     3241 tgaaaaagcc catggcagga tttattgccg ggcagacggc gcctacgggg aaacgcatgg
     3301 ggcacgccgg cgcgattatt tccggcggca aaggcacggc caaagaaaaa attgcagcaa
     3361 tgaaacaagc gggtattcgc atggcgtcgt cgccggcgga tattggccgc tcaatgcagg
     3421 aggcgatggg aaagtagtat ggccatggaa agaactctat cgattgtgaa acccgacgca
     3481 gtaaagcgta atttgatcgg cgctattctc aaaaaattcg aggccgttgg actgcgaatc
     3541 gccgccggaa aattgatccg tttgacgccg gaccaagccc aatcttttta tgccgtgcac
     3601 aaagcgcggc cgttttttca gggattgtgc gattatatgt cttcgggccc gattttcgtt
     3661 tcggtgctcg aaggtgacaa tgcaattgcc cggaatcgcg aagtaatggg cgcgaccgat
     3721 ccggcgaaag cagcgaccgg aacgattcgc caggagttcg gcgaggacat cgaaaagaac
     3781 gccgtgcacg gttccgatgc cccggagacc gccgccacgg aaattgcttt ttatttcaag
     3841 cccgacgaaa tttattaact tgtcgccata ttgggtgggg aatgtgaagg cggacggcaa
     3901 gcccaatatt aaagacctga gctccggcga gctgggcgaa tttctcgccg cggctaaaca
     3961 gccgggctac cgcgcgaaac agatatggca gtggttgttt cgcgagcatg tcaccgagtt
     4021 cgcgtccatg accaacctgc cggtgccatt gcgcgagcag ttggcatgct catttacgat
     4081 tggccggctg gcaatcgaac ggcgtcacga ttctcgtgat ggcaccgtca aatttctgtt
     4141 tggcttggcc gacggcaaga gcatcgagag tgtcgtcatc ccggaaagac agcgactgac
     4201 gatctgcatc tcgacgcaag tgggttgcgc ttatggctgc gccttttgcg ccaccgggct
     4261 gttaggcttt aaacgcaatc ttacttgcgg tgagatcgtc gagcagattc tcgaggccaa
     4321 tcgagctctt gtcgaagacc ggcgcatcac caacgtggtt ctcatgggca tgggcgagcc
     4381 tttggcaaac tacgagcaga ctcggcgtgc cctcgagatc atgatcgatc cggcctgggg
     4441 attggcaatc tcgccgcgaa aaattaccct gtccagtgtc ggtctggtgc cgcagatcca
     4501 aaaattgatg gaagagacca atatcacgtt ggcgatctcg ctgcacgcag ccactgaaga
     4561 attgcgcggt cgcttgatgc cagttaaccg caagtactcg ctggaagagt tgatcgattg
     4621 ttgccgcaac ttgccgctgc cgcggcgcaa acgcatcacg ttcgaatacg ttttgctgag
     4681 tggcgtaaac gattcgttgg ctgatgccaa agcgttatgc caggtcttgc gcggggtgcc
     4741 cagcaaagtc aacgtgattc cgttcaatcc ccatcccggt agcgaatttc agcggccgtc
     4801 cgacgacgcg gtgcagcgtt tccaacaggt gcttttcgat cacaagatcc aagtcaatgt
     4861 gcgccgcccg cgcggcgacg atatcgccgc agcttgcggt cagctgcagg ggcaggcgca
     4921 aagcgagtca ctcgataccg acgcgcgggt cgcggcgaac tagtagcatg cgccatccaa
     4981 ccatcggcgt gatcggcggc agcggccttt atcagatggc ggggctcaaa accgtgcgcg
     5041 aggtggcggt ttctacgcct ttcggcaagc cgtccgacaa attaatacgc ggcaagctcg
     5101 gtgatagcga cgtagtcttc ttaccgcggc acggaaaggg ccaccgttgg ttgcccacgg
     5161 aagttaattt tcgcgccaac attttcgcca tgaagaaatt aggcgtggaa cggattatat
     5221 cgatcagcgc ggtgggcagt ttgagagaag agatcgcgcc cggtcatctc gtcgtgccgg
     5281 accagtttat cgaccgcacg acccagcgcc cgagcacgtt ttttggcaaa ggtctggtcg
     5341 cccatgtcag catggcagac ccgttttgca acgatctctc tcgagtattg caagattctg
     5401 cccgccagag cggagcgacc gtgcatcgcg gcgggaccta tctgtgcatg gaaggaccgc
     5461 agttttccac ccgggccgag tcgcgtttgt atcgcagttg gggcgctcat gtaattggca
     5521 tgaccaattt gcaagaggca aaactcgcgc gcgaggcgga gatatgcttc gcgacattgg
     5581 ctttggcgac cgactacgat tgttggaacg agcaagccgg tgacgtcgaa atcgagcggg
     5641 tgttgacggt tttacagaac aacgtgaatc tggcgcggcg caccataggc gcggcggttg
     5701 gccttctaaa cgaaccgcga agttgcagct gtgcgagcgc tctaaaggat gctatcataa
     5761 ccgatcgtcg cgcaattccc cggaaactta ggcacgatct aaatccctta ataggaaaat
     5821 acttatgagc gtattggtgg ttggcacggt tgctttcgat tccatcgaga cgccatcggg
     5881 ctcggtggag agaatcctcg gcggctcggc atcgtatttt gcgttgggcg cgagttactt
     5941 tgcgccggtg cgggtcgtcg gcgtcatcgg tcaggatttt ccgcaggacc atctcgatct
     6001 gttcactcaa cgcggcatcg atatcgaggg gatcaagcgc gagaaaggcg acacgtttca
     6061 ttggcgcggc cgctatcatg aagacatcaa ccagcgcgac accatcgaat tgcacctcaa
     6121 tgtgttggcg ggtttcaaac ctaatcttcc ggagcgctat cgtgacacgg agtatgtctt
     6181 tctcggtaat atcgatccgt tgatacagat ggacgttgtc gagcaaatcc gccatatgaa
     6241 gcttttggtc tgcgacacca tggattactg gattcgcgag agcaacgcag cgctgaaaaa
     6301 agtcttgcag cgcatcgaga tgctggtgtt gaacgattca gaagcgcgtt tgctgagtgg
     6361 ctatgacaat attgtcaaag ctgcgcgggc gatactccgc atgggtccga aaatggtttt
     6421 gatcaagcgc ggcgaatatg gagttttgca attttccgag tctgccgtct tcgccacacc
     6481 tgcatatccg ctcgaagagg ttttcgatcc gacgggtgcc ggagactcat ttgcgggcgg
     6541 tctcatgggc cagctggccc gttccgggga cgccagccag ggcggcatgc gccgggccat
     6601 cgtctacggc tcggtggtgg cgtcattcac cgtcgaggat tttggcgtca aacggctcgc
     6661 cggcctttcc ttgccggaca tcgaacaacg ctaccaacgg tttatcgagc tgactgattt
     6721 tcattcctga gccgatggat ctttctgtcg ttattcccgt ctataacgag gaagagaatg
     6781 tcgaaccgct gatcaccgag atcgaggcag ccttggcggc gctcgggaaa aaatacgaaa
     6841 tcatcgttgt cgatgacggc agtaaagatt cgacgtttgt taagcttaga tcaattcata
     6901 aacgcgtggc caagcttcaa gtcgttcgtc tcaagcgcaa cttcggccag actgcggcaa
     6961 tcgccgccgg gctggctcac gcgcggggcg tcgtggtaat tctcatggat ggtgacgcac
     7021 agaacgatcc cgccgacatt cccgcgctcc tacgcaagct cgatgaaggc aacgatctgg
     7081 tggcgggctg gcgttacaac cgtcaagatc cctttctcaa ccggcggctg ccctcgatga
     7141 tcgccaaccg gctgatttct tctacgaccc atgtcaagct gcatgattac ggttgcactc
     7201 tgaaggcgat gcgtcaggat gtcgcaaaaa atttgcgcct ttacggcgag atgcaccggt
     7261 ttattcccgc catcgcctac gagcgcggcg cgcgcatcgc cgaattgaag gtcaatcatc
     7321 gaccgcgcct gcgcggcaca tcgaagtacg gcatcactcg gactttgcgt gtagccctgg
     7381 atctattaac ggtcaaattt cttagcagct attcgacccg gccgtcgcat gtgtttgggc
     7441 ccatcggtct gggtagcggt tttctgggtt ttgtcatcgg actttacttg accgctcaga
     7501 aaattttgta tgggcaggat atcggcggcc ggccgttgct gttgcttgcc gttctgctaa
     7561 ttttcatcgg ttttcagttt gtcaccatgg gcttgctggg tgagatgctg gcacgcacct
     7621 atcatgagtc gcaagaccgg ccggtatacg tggtcggcga agttctagga ggaggaacgg
     7681 gcgcatgaaa ttggcggtcg taggaaccgg ctatgtcggt ttggtcgcgg gtacttgtct
     7741 ctccgagagc ggcaacgacg tgatctgcgt cgacattgac gcgcagaaga tcgatctgct
     7801 caagggcggc aaggtgccga tctatgagcc gggattggaa gagatggtgc ggcgcaacgc
     7861 cgaggatcag cgcctgagct tcactacgga tctgcccagc gccgtgaaaa aatcggatgt
     7921 cattttcatc gcggttggca caccccaggg agaaaacggc catgccaatc tagaattcgt
     7981 caagtcgact gccaagagta tcgccaaggc gctcgactcg ttcaagatca tcgttaccaa
     8041 gagcaccgtg ccggtgggca cggctgatca gatcaaaaaa tggatcgccg aagaaaccgc
     8101 acaacccttc gcagtggcat ccaatcctga gttcatgaaa gagggcgccg cggtggagga
     8161 cttcatgaaa cccgaccgtg tcgtgttggg cggcgacgat ccggcagcgt tggcgacgct
     8221 gcgcgagctc tatgatccat ttgtgaggac gggtaatcca attctcatca tggacagccg
     8281 cagcgccgaa atgagtaaat atgccgccaa cgccatgctt gcgacgaaga tttcctttat
     8341 caatgaagtt gcccgcctgt gcgaacagat gaatgccgac atcggcgaag tgcgccgggc
     8401 gatcagtcta gaccgacgca ttgggccgca ctttattttt cccggtgtcg ggtatggcgg
     8461 ttcgtgcttt cccaaggata cgcgcgccat gattggcatg ggcggcgatc atcaagacat
     8521 ggttttgctc agggccgtcg aacaggtgaa cgaacggcag aaacagttgt tattcgacaa
     8581 agtcgggcgc cattttggtg gtaaactctc agggctgacg tttgccgtct ggggtttggc
     8641 tttcaagccg cgcacggacg atatgcgcga tgccccggcg atcaatatca ttgaatcttt
     8701 gctcgcagcc ggagccaggg tgcaggcttt cgaccccgag gcgatggaag aggcgcgcaa
     8761 aatattcggc gatcgcatcc aatacacgca ccgcaattac gacgcgctcc atggtgcggc
     8821 cgctttgttg atcgtcaccg aatggaatga gttccgccgc ccggattttg ttcgcgtcaa
     8881 gaatctgctc accagcccgg tgatcttcga cggacgcaac atctacgatc ctgcggatct
     8941 gcgcaagctg ggatttactt attattcgat tgggagaaat gatggctaga tatttgatta
     9001 ccggcggtgc tggttttatc ggctcccacc tatgtgaata ttttctcaat caaggccatc
     9061 aagtgttatg catggataac tactccaccg gcgccaatga aaacatcggg tcactattaa
     9121 agaatccccg cttccgcttt atcgatcaca atgtcagccg ctacatcgaa gttccggagc
     9181 cgctggattt tgtcttgcac ttcgcttcgc cggcaagtcc ggtcgactac ttagagctgc
     9241 cgattccaac cttgaaagtc ggcgccttgg gtactcacaa caccctcggg ctggctaaag
     9301 ccaagagcgc aacttatctg ctggcgtcga cttcggaagt ctatggcgat ccgctggtgc
     9361 ggccgcagag cgaagagtac tggggcaacg tcaatcccat aggaccgcgc ggtgtttatg
     9421 acgaagccaa acgcttcgcc gaagcgatca ccatggcgta tcatcgatac catggcctcg
     9481 acagccgcat cgtgcggatt tttaatacct acggcccgcg catgcgcatc cgcgacggca
     9541 gggtggtgcc caacttcatc atgcaggcat taaaagacga gccgttgacc gtatacggcc
     9601 aggggcaaca gacgcgcagc tttcagtacg tcgatgatct catcgccggt cttgataagt
     9661 tactgcagtc caatcatcat ctgccggtca atatcggcaa tccttatgag atgacggttt
     9721 tacagttcgc caataaaatc atcgaaatga ccaaatccaa gagcgaaatc gtttacaaac
     9781 cactgcccga agacgacccg caggtacgcc agcccgatat cactaaagct cgaaaaattc
     9841 tcgggtggga gcccaaggtg aaactggaag aaggtttggt caagaccatc gaatactttc
     9901 gcacgaagct ttaggattga tctgatgcgg atcctggtta ctggaggagc gggctttatc
     9961 gcctcgcaca tcaccgacgc atatatcgag cgcggccatg aagttcatat cgtcgacgac
    10021 ttctcaacgg ggcagcgcgc caacctcaat ggtaaagcta ccttgcacca gatcgatatc
    10081 gccgacagtc acagtgttaa gttagtcgag cggatcaaac cggatatatt gagtcatcac
    10141 gcggcgcaga tggatgtgcg ccactcggtg gccgatccga cattcgacgc gcgcgtgaat
    10201 attctcggct tcatcaattt actggaaggc tgtaagaacg ccggcgtcaa aaaagtgatt
    10261 ttcgcctctt ccggtggcgc ggtttacggc gagcaggaga gttttcccgc ttcagaaagc
    10321 catcctacgc aacctgccag tccgtacgga gtgagcaagc ggacgggaga attgtatctg
    10381 tcctattatc accagacatt tggcctgccc tatctcgctt tacgctatgc taatgtttac
    10441 ggcccacgcc agtcgaccca gggagaggcc ggtgtggtgg caatattctt ggcgctgctg
    10501 ttaacgggca agacgccggt catcaatggc gacggcaggc agacccgtga ttacgtttac
    10561 gtcggcgatg tagtcgcggc aaacgtcggc gcactacaga gcgactttgt cggcccggtc
    10621 aacatcggca ccggtgtcga aaccgacgtg gttacgattt acgatcatct acgcgttgcc
    10681 gtcggcagcg acatgcacgc ccaacacggc ccggcaaaag ccggcgaaca gcggcgcagc
    10741 tgcatcgata atcggcgcgc cggtgaagtt ttagggtggc gcccgcaagt ggcgctgcag
    10801 gatggcctgc aacacaccgc agccttttat cgcgaacatt taactcaatg aaactcgatc
    10861 atatccgcaa tttttcaatc attgcccata tcgatcacgg caagtcgact ctggcggaca
    10921 ggcttttgga gttcaccggg gcggtgagcg agcgggagaa gaccgaccag atattggaca
    10981 gcatggaact cgaacgggag cgtggaatca cgatcaaggc tagtccggtg tgtttacgct
    11041 atcgagccaa ggacggcaaa gagtacaagt tcaacttgat tgacactcca ggccatgtcg
    11101 atttcaccta tgaggtctcg cgcagcctgg ccgcttgcga gggggcgttg ttgatcgtcg
    11161 atgccgccca gggtgtcgaa gctcagactg ttgccaatgt tcatatggcg ctggatctca
    11221 atctcgaagt cctgccggtg atcaacaaga tcgatttgcc cagcgccgag ccggaacggg
    11281 tcagacaaga aatcgaggat gtcatcggtc tcgatgcctc gaatgcgatc cttgccagcg
    11341 cgaaagaggg cgtcggcatc gaagatattc tcgaagcggt gatccacttt tttccgccgc
    11401 cccagggcga tgccaatgcg ccgttgacgg cgtcgatcat cgattgttgg ttcgaccctt
    11461 accacggcgc ggtggtg
//
LOCUS       DATLCH010000061         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1690956, whole genome shotgun sequence.
ACCESSION   DATLCH010000061 DATLCH010000000
VERSION     DATLCH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690956"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1152)
                     /locus_tag="VMZ02_01745"
     CDS             complement(124..1152)
                     /locus_tag="VMZ02_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70678.1"
                     /translation="MAVIDSDAHVLETEHTWDYMLESERSLKPRIVPTPNDPTSGGES
                     WLIDGLYLGKARNVGADTSREAREMGDINARLKHMDELDIDIQVLYPTFCLRPFTRRP
                     DVELALARSYNRWLIDIWKVAPERLRWVALLPLLSMDKAIEEARYAKEHGACGIFIRG
                     LEGEKRLHDAYFYPLYEEAGKLDLPICVHSATGSFAVHDYFGDECGFNRFKLAVVGSF
                     HSLIFYKVPEKFPKTKWAFIEVSSQWVPYAIHDYARRYERNGTKIDKSEVLRKNHIWV
                     ACQTDDDLPYVLKFSGDDMLVIGTDYGHNDTSSEILALRKLKEDGAIPKSVVNRILDD
                     NARALYGL"
     gene            complement(1155..1373)
                     /locus_tag="VMZ02_01750"
     CDS             complement(1155..1373)
                     /locus_tag="VMZ02_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70679.1"
                     /translation="MAESYGQFNASLLYCNKCGQAMPVRQRLLLVLPDGELYDYTCQK
                     CNNSVGSKTEKAGINQSSGAAKLRSGGF"
     gene            complement(1382..>3011)
                     /locus_tag="VMZ02_01755"
     CDS             complement(1382..>3011)
                     /locus_tag="VMZ02_01755"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUR70680.1"
                     /translation="QMPLGAEFGYKGVVDLLSMKAFVFDGDSGKCTEVDIPSELSADA
                     LEWREKMLEDVSELDDGLLEKFLDGKELAPEELKKAVRQATLERRIFPIVFGSPLRQI
                     GVVQLLDAVTEYLPAPLEEGEFTGVNPVKGEALKRKHDPDAPFSAYVFKTVIDPFAGK
                     LSILRVLSGKLNSDMTIYVPARQVREKVGHLFRLEGKKQEAVKEALAGEIVAVAKFRD
                     IATGDSLCDEKAPIQYDGPVHFTPNISFALEPKSKADEDKLPQGLHRMKEEDQTIDLH
                     RDEETRDFILSGMGQQHIEIAVEKLKRKYGAEVVLKAPKVPYKETIRGSASAQGRQKK
                     QTGGHGQFGDTWIKVEPLPHGGGFEFVDKIVGGAIPRNYIPAVEKGIREAMAGGYLAG
                     YPMVDVKATLYDGSYHDVDSSDMAFKIAASMGFRNAVEKAKPVLLEPIMNLEVSVPDE
                     CMGDVIGDLNSRRGKVVGMETKGHIQVIKSKVPMAEVLKYAADLRAITSGRGEFQLEF
                     GHYEELPAHLAEKVIKETKARKMAEHDGEHKAHG"
BASE COUNT          627 a          881 c          789 g          714 t
ORIGIN      
        1 gggcgcttcg acactcaccg cgccgtcgag ccgctgcatc gcggcgagat aactctccgg
       61 aaatagatgg gcttggaaat cgatgatcat gcttggttgc cgctccagaa aacttactga
      121 aggttatagc ccgtacagcg cgcgcgcatt gtcgtcgaga atgcggttca ccaccgattt
      181 cggaatcgcg ccgtcttctt tgagcttgcg caatgccaat atctccgacg atgtgtcgtt
      241 atggccgtag tcggtgccga tcaccagcat atcgtcgccg gaaaatttaa gcacgtacgg
      301 caggtcgtca tcggtctggc aggcgaccca aatatggttt ttgcgcaaca cttctgactt
      361 gtcgattttg gtgccattgc gttcgtagcg ccgggcataa tcgtggatcg catagggcac
      421 ccactgtgag ctgacttcga tgaatgccca tttggttttg ggaaattttt ccggaacctt
      481 gtaaaaaatc aacgaatgaa atgagccgac cactgccaac ttgaagcggt tgaagccgca
      541 ttcatcgcca aagtaatcat gcacggcaaa actaccggtg gccgaatgga cgcaaatcgg
      601 cagatcgagc ttgccggcct cttcgtaaag cggataaaag tacgcgtcat gcagccgctt
      661 ctcgccttcc aggccgcgaa tgaagatgcc gcaggcgcca tgttccttgg cgtagcgcgc
      721 ctcttcgatg gctttatcca ttgacagcag cggcagcagc gctacccagc gcaagcgctc
      781 cggcgcgact ttccagatgt cgatcaacca acgattgtaa gagcgcgcga gcgccaattc
      841 gacgtccggc cggcgggtaa acggccgcag gcaaaaggtc ggataaagaa cctgtatatc
      901 gatgtcgagc tcgtccatgt gcttcagacg ggcgttgata tctcccatct cgcgcgcttc
      961 gcgcgaggta tcggcgccaa cattgcgcgc tttgccgaga tagagcccgt cgatcaacca
     1021 cgattcgcca ccggatgtcg gatcgttggg cgtcggcacg atgcgcggtt tgagcgaccg
     1081 ctcggactcc aacatatagt cccaggtatg ttcggtctcg agcacatggg catcagaatc
     1141 gatcactgcc atccctaaaa tcctcctgaa cgcagcttag cggcaccgct gctttgatta
     1201 attccagctt tctcggtttt cgagccgacg gaattattac acttctgaca agtgtagtcg
     1261 tatagttcgc catcgggtaa aacaagcaac aagcgctggc gcaccggcat tgcctggccg
     1321 catttattgc aatagagcag actggcattg aattgaccgt aggactccgc cattgcgtca
     1381 cctaaccgtg ggctttgtgt tcaccgtcat gctcggccat tttgcgtgcc ttggtctctt
     1441 tgatcacttt ctcggccaag tgtgccggca gctcttcata gtgcccaaac tccagttgaa
     1501 actcgccgcg cccgctggtg atggcgcgca gatcggcggc atatttgagc acctccgcca
     1561 ttggcacctt gcttttaatc acttgaatat gacccttggt ttccataccc accacttttc
     1621 cgcgccggct attgagatcg ccgatgacat cgcccatgca ctcgtcgggc acggaaacct
     1681 ccaggttcat gatcggctcc agcagaaccg gtttggcttt ctcgacggcg ttgcgaaagc
     1741 ccatcgacgc ggcgattttg aacgccatat cggacgagtc gacatcatga taggagccgt
     1801 cgtagagcgt cgctttgaca tcgaccatgg gatagccggc taggtagccg cctgccatcg
     1861 cttcccttat ccccttctcc accgccggga tataattccg cgggatcgcg ccgccgacaa
     1921 tcttatcgac gaattcaaac ccgccaccat ggggcagcgg ttcgactttg atccaggtat
     1981 cgccgaattg cccatgaccg ccggtctgtt ttttttgcct tccttgggcg ctggcactgc
     2041 cgcgaatggt ttctttatac ggcacttttg gcgctttcag caccacctcg gcgccatatt
     2101 tacgtttcaa tttttcgacg gcgatttcaa tatgctgctg acccatgccc gacaaaataa
     2161 aatcccgggt ctcctcatcg cgatgcagat cgatagtttg gtcctcttcc ttcatgcgat
     2221 gcagtccctg cggcagcttg tcctcgtctg ctttgctctt gggttccagc gcgaaggaaa
     2281 tattgggcgt gaaatggacc ggcccgtcat attgaatcgg cgccttttca tcgcacagac
     2341 tgtcgccggt ggcgatgtct ctaaacttgg cgacggcgac gatctcaccc gccagcgctt
     2401 ccttgaccgc ctcttgcttt tttccctcca ggcgaaataa atggccgacc ttctcgcgaa
     2461 cttgccgggc cggcacgtaa atggtcatgt cggaattgag cttgccggag agcacgcgca
     2521 ggatcgacag cttaccggca aatggatcga tgacggtttt aaaaacataa gctgagaatg
     2581 gcgcgtcagg atcatgtttg cgcttgagcg cttcgccttt cacgggattc acgccggtga
     2641 attctccctc ctccagcggc gccggcagat actcggtcac ggcgtcgagc aactggacga
     2701 cgcctatctg gcgcagcggc gaaccgaaaa caatcggaaa tattcggcgc tccagagtgg
     2761 cctgacggac agcttttttt aattcttccg gtgccaattc cttgccgtcg agaaacttct
     2821 cgagtaatcc gtcatcgagt tcggagacgt cttcgagcat tttctcgcgc cactccagag
     2881 cgtccgcgct gagctccgag ggaatgtcaa cttcagtgca tttgcccgag tcaccatcaa
     2941 agacgaaagc cttcatcgac aagagatcga caactccctt gtagccgaat tcggcgccga
     3001 gcggcatctg c
//
LOCUS       DATLCH010000062         4886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1691816, whole genome shotgun sequence.
ACCESSION   DATLCH010000062 DATLCH010000000
VERSION     DATLCH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691816"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VMZ02_01760"
     CDS             complement(<1..219)
                     /locus_tag="VMZ02_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70681.1"
                     /translation="MNSFAKVVAWALVVISLQSTVGAETLEQLIAGAKKESELTFIAG
                     AQTFGGQKTLNILEAAFNKRFGLNMKIRF"
     gene            complement(334..2082)
                     /locus_tag="VMZ02_01765"
     CDS             complement(334..2082)
                     /locus_tag="VMZ02_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR70682.1"
                     /translation="MPARYGSDLIVDLMKVLGIEAVALNPGSSFRGLHDSLVNYESGT
                     KPNPEMILCCHEEIAVAVAHGYFKATGKIMPAIVHNVVGLQHASMAIYNAWCDHTPVM
                     VMGGTGPMNSSKRRPWIDWIHTAQVQGNLVRDFVKWDDQPVGVEAFSSSMMRGYRMAM
                     SDPGGPVYLCFDVSDQEAAIDKEIPLPDPNRFRPAAPLQADYDAIKQTAQLLATAQSP
                     VILADYVGRNSDAVMSLVEVADLLSIPVIDFGARLNFPNTHSLNLTGRNRELIANADV
                     IMGLDMTDLYGALMQSDPNTHANVPATKSSCKVINVTLKDIVVRSWTSDFQELAPVDV
                     PIVANTKVFLPALAEEIRKQGKFSKTVVEDRLKALTAQHQEVHARWQADLKKRWDQRP
                     ISPPRLAWEIWEALKNEDWLLVAGAFRGWPSRLWTWDKPGLFLGGYGGGGLGYGPAAS
                     VGASLPYRGTGKICVNLQRDGEMLYTPSSLWTAANTGVPLLTVMTNNRVYYNDVEHQE
                     KVAIHRGRPVENKLVGMEMGKPPVDFANLARTFSVHGEGPITEPEQIRPAVERAIKVI
                     KEEKRPALVDVIMQMI"
     gene            complement(2195..3259)
                     /locus_tag="VMZ02_01770"
     CDS             complement(2195..3259)
                     /locus_tag="VMZ02_01770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70683.1"
                     /translation="MAGNGSDAGFEVIISSDSHVMEPSEIIVERAPKAFKDQVPRFPN
                     LKVGSSFQTHPGGSDPTQRIKEMETDGLSAEVLYPTYLLPQYAMDDARLQEVTFRAYN
                     DWLIEYCNSSPKRLIGIAAIAVYNIDEAVKELERCAKAGMKGSLIWQAPHPDLPLHSE
                     HYNKFWAASQDLNMPVHLHILTGHGYHKTTVFGNKRTGVEAYRGSVNLKLKEVIDDVF
                     ELIFYGVLERYPGLKIVSVENEIGWMPFMMQQWDYYYNRFKKVNPPPFTKNPSEYFKT
                     QIYGTFFRDTVAGHNFQWWGQDNCMWSNDYPHGNSTWPESKKYIDRDLGHLPEDIRKK
                     LVYTNVKQLYGIDIPHPSVH"
     gene            complement(3461..4120)
                     /locus_tag="VMZ02_01775"
     CDS             complement(3461..4120)
                     /locus_tag="VMZ02_01775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUR70684.1"
                     /translation="MALHINEEAPNFTAETTEGKINFHEWIGNGWAILFSHPKDFTPV
                     CTTELGYMAKLKSEFEKRNCKVIGLSIDPVTDHAKWAKDIEETQGHAVNYPIIGDSDL
                     KVAKLYDMIHPDTPGSSAGRTAADNQTVRSVFVVGPDKKIKLMLTYPMTTGRNFDEVL
                     RVLDSMQLTSKHQVATPVNWKQGDDVIIVTSVSDEDAKKKYPQGWKALRPYLRLVSQP
                     K"
     gene            complement(4238..>4886)
                     /locus_tag="VMZ02_01780"
     CDS             complement(4238..>4886)
                     /locus_tag="VMZ02_01780"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70685.1"
                     /translation="FMVNPSIKTPADLKGKKIGVQSIGGGVWMFSMLAFEHWGLNVER
                     DNIQFRIIGDQSVLVQAITQNIIDGGYLGYAYGAQLERQGYRMLGDLMKMGIPYQGLG
                     MIAKRSLLDRTPEVPERTLRAMVETISYINNPANKAAVVRTLAKELRLAKVEDAQAGY
                     EVMRTLYDKKIYPNVEGLRNVVRLLGRTSEPIRKIKVEEIIDDRVVRKLEKEGLF"
BASE COUNT         1055 a         1416 c         1250 g         1165 t
ORIGIN      
        1 aaagcgtatc ttcatgttaa ggccgaagcg cttgttgaac gccgcctcaa ggatatttaa
       61 tgtcttctgc ccgccaaaag tctgcgcccc agcgatgaaa gtcagctccg attctttctt
      121 agcccctgcg atcaactgct ccagggtttc cgcgccaacc gttgactgca gcgaaattac
      181 caccagcgcc caggcgacaa ccttggcaaa agaattcatc gcgctctcca atccgaaaac
      241 ctttgcacct tgaacccttg aacttttgaa cgaatccgat ccctggacga ttggaacacc
      301 tggaaccttt ggaacgattg aaaccggtat taatcaaatc atctgcataa tcacgtcgac
      361 caacgccggc cgtttttctt ctttgatgac cttgatcgcc cgttccaccg ccggccgaat
      421 ttgttcaggt tcggtgatcg gcccctcgcc gtgcacactg aaggtgcgcg ccaggttggc
      481 aaaatccacc ggcggtttgc ccatctccat gccgacaagt ttgttttcca ccggccgtcc
      541 ccggtgtatt gcgacttttt cctgatgctc gacgtcgttg tagtaaaccc gattgttggt
      601 catcacggtc aacaacggca caccggtatt cgccgccgtc cacaaggagc tcggcgtgta
      661 gagcatctcg ccatcgcgct gcaggttgac acagatctta ccggtgccgc gatagggcag
      721 cgatgcgccg acagaggccg ctggaccgta gcccaatcca ccgccgccat aaccaccgag
      781 aaatagcccg ggcttgtccc aagtccatag ccggctcggc cagccgcgaa aggcgccggc
      841 gaccagcagc cagtcttcgt tcttcaaagc ttcccaaatc tcccaggcaa gacgcggcgg
      901 cgagatcggc cgttggtccc agcgtttttt gagatcagct tgccagcgcg catgaacttc
      961 ctggtgttgg gcggtcagtg ccttaagccg gtcttcgact acggtcttgg aaaactttcc
     1021 ctgttttctg atttcttcgg caagcgctgg gagaaaaacc ttggtgttgg caacgattgg
     1081 cacatccacc ggcgccaatt cctgaaagtc gctggtccag ctgcgcacaa cgatatcttt
     1141 gagtgtcaca ttaatcactt tacagctgga tttggtcgcg ggaacgttgg cgtgcgtgtt
     1201 gggatccgac tgcatcagag cgccgtacag gtcggtcatg tccagtccca tgatgacatc
     1261 ggcgttggcg atcaactcgc gatttctacc ggtgagattc aaagaatgag tgttgggaaa
     1321 attcaagcgc gcgccaaaat cgatcaccgg aatcgacaaa agatccgcga cctccaccag
     1381 actcatcacg gcatcgctgt tgcgccccac gtagtcggcc agaatcaccg gactttgtgc
     1441 ggtcgccaat agctgcgccg tttgtttgat tgcgtcgtag tccgcctgca gcggcgctgc
     1501 cggacgaaat cggttcggat cgggtagcgg tatttccttg tcgatcgcgg cttcctgatc
     1561 gctcacatcg aagcagagat aaacggggcc accgggatca ctcatggcca tgcgatagcc
     1621 gcgcatcatc gaggacgaaa aggcctcgac gccgaccggc tggtcgtccc acttgacgaa
     1681 gtctcgcacg agattaccct gcacttgcgc cgtgtgaatc cagtcgatcc agggacggcg
     1741 cttgctggaa ttcatcggcc cggtaccgcc catcaccatc accggcgtgt gatcgcacca
     1801 ggcgttgtag atcgccatgg aggcgtgctg cagaccgacg acgttatgga cgatggccgg
     1861 catgatcttg ccggtggctt tgaagtaacc gtgggcgacc gctacggcga tctcctcgtg
     1921 gcagcaaaga atcatttcgg ggttgggctt cgttccactc tcatagttga ccaaagaatc
     1981 atgaaggccg cgaaaactgg aacccgggtt cagcgccacg gcttcgatgc ccaacacctt
     2041 catcaaatcg acgatgagat cggatccgta acgagcaggc ataaaacctc ctggacggta
     2101 aattaatcgc tcaaagccgg ccaaaaaaaa ggcgcgctag cagacttgga cgggtccgtt
     2161 agcgcgccat ttcagtcacc gcgacgatgc cgctttaatg cacagaagga tgcggaatat
     2221 cgatgccgta aagctgtttc acgttggtgt agaccaactt cttgcggata tcctccggca
     2281 gatggcccaa gtcgcgatca atatacttct tcgattccgg ccaggtcgag ttgccatggg
     2341 gatagtcgtt cgaccacatg caattgtcct gaccccacca ttggaagtta tggccggcca
     2401 ccgtgtcacg gaaaaatgta ccgtagattt gcgtcttgaa gtattcgctc gggttcttgg
     2461 taaacggcgg cgggtttact tttttgaagc ggttataata ataatcccac tgctgcatca
     2521 tgaacggcat ccagccgatc tcgttttcca cgctgacgat cttcaatccg ggataacgtt
     2581 cgagcacgcc gtaaaagatc agctcgaaga cgtcgtcgat aacttctttg agtttgagat
     2641 tcacgctgcc gcgatacgcc tcgacgccgg tccgcttgtt cccgaaaacc gtggtcttgt
     2701 gatagccgtg gccggtgagg atgtgcaagt gcaccggcat attgagatcc tgcgacgccg
     2761 cccagaattt gttgtagtgc tccgaatgca gcggcagatc cggatggggc gcctgccaga
     2821 tgagcgaacc tttcatgccc gccttggcgc agcgttcgag ctccttcacc gcctcgtcga
     2881 tattgtagac cgcgatggcc gcgatgccga tcagccgttt cggcgaactg ttgcagtatt
     2941 ctatcagcca gtcgttatag gcgcggaacg tgacttcctg cagtcgagcg tcgtccatcg
     3001 cgtactgggg tagcaaatag gttggataga gcacttccgc ggagagccca tcggtctcca
     3061 tctcttttat ccgctgcgtc gggtccgagc cgccggggtg ggtctgaaag ctactaccga
     3121 ccttgaggtt gggaaagcgc ggcacctggt ctttgaacgc cttgggtgca cgctcgacga
     3181 taatttccga cggttccatg acgtgagagt ctgacgaaat aatgacttcg aaccccgcat
     3241 cgctaccgtt gccagccata tcttttcctc ctcggaatca atctgcaaat ttaatcattg
     3301 cctacaaacg atggtctacc accatcgcta ttcaagggtc aagattcacc agcatggcta
     3361 aacacacccc aatcagttga ctgactcgga accgaacttt gtgaaagctt cgaggctaaa
     3421 gaagcgccct tggcaccccc tttagcttta gtcgcggaaa ttacttcggt tgcgagacca
     3481 gccggaggta aggccgcaat gctttccagc cctgaggata tttcttttta gcgtcttcgt
     3541 ccgacaccga ggtcacgatg ataacatcgt caccctgttt ccagttaacc ggtgtggcca
     3601 cttggtgctt cgaagtaagc tgcatcgaat ccaacacccg caggacttcg tcaaaattac
     3661 gtccggtcgt catcggatag gtcagcatca gcttgatctt cttgtctggc ccgacgacga
     3721 aaaccgaccg tacggtctga ttatcggcgg cggtacgtcc cgccgaactg ccgggggtat
     3781 cgggatgaat catgtcatac aattttgcta ctttgaggtc tgaatcaccg atgattggat
     3841 agttcaccgc atgtccttgg gtttcctcga tatccttggc ccattttgca tgatcggtca
     3901 ccgggtcgat gctcagcccg atcactttgc agttacgctt ttcgaactcg ctttttagct
     3961 tggccatata accgagctca gtggtgcaca ccggcgtaaa atccttggga tgagaaaaca
     4021 aaatcgccca gccattgccg atccattcat gaaaattaat ctttccttca gttgtctctg
     4081 cggtaaagtt cggtgcttcc tcattgatat gcagtgccat cgtccaaccc tccttcgaaa
     4141 atagatatgg ttgattttgc cttctctcac cctcaacatc tggcccatga ctaacagatc
     4201 gaaaccctaa atcaaatcta atcgtccttc ctgccggcta gaacaatcct tctttttcga
     4261 gcttgcgcac cacccggtca tcgataatct cttcgacttt aattttgcgg atcggctcgc
     4321 tggtgcggcc gagcagacgc acgacgttgc gcagcccttc gacgttggga taaatcttct
     4381 tatcgtagag tgtgcgcatc acctcgtatc ccgcctgcgc gtcttcgact ttcgccaacc
     4441 gcagctcctt ggccagagtc ctaaccaccg cggctttgtt cgccggatta ttgatatagc
     4501 tgatggtttc caccatcgcg cgcaacgtgc gctctggaac ttccggcgtg cggtcgagca
     4561 aactgcgctt ggcgatcatg ccgagaccct gatagggaat gcccattttc atcagatcac
     4621 cgagcatccg atagccctgg cgctccagtt gtgcgccgta cgcgtagccg agatatccgc
     4681 catcgatgat attctgggta atcgcttgca ccagcacgga ttgatcgccg atgatgcgga
     4741 actggatgtt atcgcgctcg acattgagcc cccaatgttc gaaggccagc atcgaaaaca
     4801 tccacacccc gccgccaatg ctctgcacgc cgatcttctt acctttcaaa tccgccggcg
     4861 ttttaatgct tgggttgacc ataaac
//
LOCUS       DATLCH010000063        10438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6812910, whole genome shotgun sequence.
ACCESSION   DATLCH010000063 DATLCH010000000
VERSION     DATLCH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6812910"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(691..2220)
                     /locus_tag="VMZ02_01785"
     CDS             complement(691..2220)
                     /locus_tag="VMZ02_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUR70686.1"
                     /translation="MADDTDSVGFYAGEVLDRTAARQPDKTAIITKDEELTFAALNHR
                     VQALAGHLIDEGIRQGDRVGVLLPNSMAIPLAYYSTQKIGAVTVILDARLKGKELEGV
                     LKDADLKLLVVHQQLYPEVEEIFKTMTPIPVWLACGSDERSFEQRFSAAKALTRPPNL
                     NPNDDALILYTSGTTGEPKGVVLSYGNLVQYPKVMREMGVTDVTTIRGCVLPMSHIVG
                     PVVCNELAEKGYTLVIFDQINPITLLEGIQKYRVNVFESVPIVFQLLLGVKNLANYDT
                     SSMKIAAMMGTSIPMPLLRAFQAAQPHIKVIQGYGLTETSPMITLVDPDKAETKMGSI
                     GRAVPGVEVRIVDESDKDVAPGEAGEIITRGPHVMKGYFRRTDATAERIRKGWLYTGD
                     VGKLEADGYFYHLGRRDDMIITGGLNVYPAEVENLIYTYPDVQETIVFAVPDAKRGQV
                     IGAAVVPRTGATVVEKDLLTFLRANLANFKVPDRIVMRDSLPRTSSGKTIRDAATLLA
                     Q"
     gene            complement(2196..3194)
                     /locus_tag="VMZ02_01790"
     CDS             complement(2196..3194)
                     /locus_tag="VMZ02_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR70687.1"
                     /translation="MAKIDDDMIRWGKLLFERRLISGWGGNLSCRLGKNAFLITGQHA
                     PLGFLAAKDLVRIHADGKPVKKSERASSETPMHLAVYAGTDAQAIVHVHPPMVLAFSL
                     THDSFAPLSYEEKYTIGKVPIIPQDTPTVTNPEPVVEALKYNPIAIIKGHGTVAIGKT
                     LQEGFLYTDLLEEAVHCQIFQNGSGVTRKHNGSQPSESIETSASAATAYELFSRAHMA
                     TLVHSANSDGDFRQFGSATGLTTSLTLHMEDSEAAWTVQFVGGEITALTEESDGDFLI
                     SGKAEWWRAVFSNRIDAFLATQQGKLELKRGDLAQLSRWYKPFQRAFALWQTIPIQ"
     gene            complement(3207..4724)
                     /locus_tag="VMZ02_01795"
     CDS             complement(3207..4724)
                     /locus_tag="VMZ02_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70688.1"
                     /translation="MSAVPSPLFEPVLWHDDGFEILDEIQVPQQIRYIHVTDVAEALD
                     AVRQMKTRAFGQVLTFLYSAALLAQNYHCTEAASLRGQLQQLTQQFCDARPTFDFAGL
                     GIMVDDFFDELPAGADVGATIATKAREFGQQIVRGRSGRAKRAAMLLPTPARLLTHCN
                     VSGELVAVAAYCKQMGKEFAVIATETRPYLQGARLTAWELAQAGVEVILIPDSAVAHT
                     LAQGEANAVIVGADRVAQNADVINKVGTYPIARMAKEYGVPFYALVQDPRSLPRGADV
                     PIEERPADELLTFHGRDLLAGDHAQLLVRYPSFDVTPAEYITQLIGFAGVYTPESFRL
                     KYHTHTASSVIETRTGGKYLLIYGVPPPSQFSFLLSALKAEGARSLLIPEMRPQLWGA
                     RHIAPELLKHNIPTTLISDNMMGTLFAHGEIAKLCFFYDRLAAEGPQGICGSLLAVQL
                     ARLHGVQIELFSADVRNEPTIDRDVSTFLGEPICPAGVTVRGLSAEAIPWALLKS"
     gene            complement(4739..5473)
                     /locus_tag="VMZ02_01800"
     CDS             complement(4739..5473)
                     /locus_tag="VMZ02_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70689.1"
                     /translation="MIKESHFTPQRMLTMLGLKANSLGPYALVPGPKERSEAMLKLLE
                     DRQKSFTFLDYEMHTGRLDGKRVSIGNGGRFAPDTAITTEILCGAGAESLIRVGSCGS
                     LQDSVKIGDLVIVTGALRGDGASRYYVAENFSTVADAGIIAALKRAAEECQVRYHLGW
                     IFTTDALFQETPELIQQLNQQNVSSIDMVTATFLTISQVRGKKAGAVLAVSDECLYGK
                     FGFRDPGFFAAEQKTIEVARQALKYL"
     gene            complement(5476..6690)
                     /locus_tag="VMZ02_01805"
     CDS             complement(5476..6690)
                     /locus_tag="VMZ02_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HUR70690.1"
                     /translation="MADQLPKLIGLQRVKKWLYAKGLEERSSGCVLCGSCYGHGPANP
                     MEDVPGPKEKCPPYEYYKFQRHTPKSRWLMAQRVFHGLDQITPELKEVIYACTNCLMC
                     QELCGVRGDGYGPWDITVAMREEITEKDGPLPAHRAIYDGLKQQDNPWGQTAHGGWAE
                     GLGLKQLGADKTSTLLFAGCSSDRASGKSGAIALAKLMQLAGEDFAVLGEQEKCCGLY
                     AFDLGFRREYERLKDLNLSMLTQAGVNKVIVACGSCQRIWREYAKGTNGLEVMHGVEF
                     IAQAVKSGQLRFTKPIDMRVTYHDSCHLGRGCGVYGPPRDILQAIPGIKVLEMERNQR
                     WAWCCGGGGGVPEADPELAQWNAAERMREALASGAEIMLTSSALCQRSFHNLSEAGLP
                     TQDLLDFILPAL"
     gene            complement(6863..7315)
                     /locus_tag="VMZ02_01810"
     CDS             complement(6863..7315)
                     /locus_tag="VMZ02_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HUR70691.1"
                     /translation="MTEVKRPFFTSYSTSLWVRFLRFSLRSHKKLEDDLLVLGLTPPQ
                     FYVLATIGYAGSLPFGEIGAKMLVTVSNLTGIVDRLEDKKFVIRKRDDKDRRVVHVML
                     TDKGAKLYKSTIPLFEKCVGEIFAGLSKGDQKHLSALLKRLDQEPAAE"
     gene            7448..8179
                     /locus_tag="VMZ02_01815"
     CDS             7448..8179
                     /locus_tag="VMZ02_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR70692.1"
                     /translation="MKLHILGCGDAFGSGGRNQSGYLVEAKDKLFLLDCGPTTLLSLK
                     RAGFDPRAIEAILLSHLHGDHFGGIAFFFLEYLYLQPRLEPLVIAGPPGTEAKVRALF
                     DVLYGGGTDREIPPTRFVVLWPDEPADINGIVIEPFRVTHQSEAISLGLKVSYEAKII
                     LFSGDSIWTEHFVELAAGVDLFLCECSFFREQPGMHVNYQDVAANLGRLRCKKLILTH
                     LGEEMLSRRSDISAVIAEDGMVLEV"
     gene            complement(8193..9362)
                     /locus_tag="VMZ02_01820"
     CDS             complement(8193..9362)
                     /locus_tag="VMZ02_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HUR70693.1"
                     /translation="MAILVVLTSVSSLWSFSPDDEITAKAALLMDAATGRILFQKDAD
                     LRLPPASTTKVMTAILALESGRKLNESLTVSKDATRVPPTKLYLRPGQTVSIEELLYG
                     IMLASANDASVVLAEGISGSVERYTDLMTKKAQSLGATNSHFINPHGLTAPDHYSTVR
                     DLATIFRYAMKIPLFREIIHTKFSSVSSTAVVKKKVVPRRISVRNHNRLLWDFDGALG
                     GKTGYTHAAQRCYVGAVQRDGVTLIVSILGATNMWSDAKHLLEYGFDNFDTLKSAHST
                     QKLLSTEAAERQRVSTVILPGDNQRSKLLTGYILQVGAFRERERAELVSRQISEMGFE
                     PVIDTGAVGRGEAAYRVHVGPYTELIAAQEVAQVILAKNGYRSLILQIPAMREAS"
     gene            complement(9594..9749)
                     /locus_tag="VMZ02_01825"
     CDS             complement(9594..9749)
                     /locus_tag="VMZ02_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70694.1"
                     /translation="MICAKTSEVLQGGICQPCQERIRREALGEQAGIAERAEREISRQ
                     GIAPKKT"
     gene            complement(9818..10345)
                     /locus_tag="VMZ02_01830"
     CDS             complement(9818..10345)
                     /locus_tag="VMZ02_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70695.1"
                     /translation="MARAAVKIWVSLLIFGAGFFTHVLYLRWNPLPVRVSSSKAPEES
                     ASMPGQAHRVNYMPLAPSAAEPEQAAALPALVARDVEKLRALTGKQARVRGRVFRVGH
                     SAKSNTYFLNFGPSREALTAVIFSPAVELFEKSQLLPKSFENKDVEITGQIKDHPQYG
                     LELVLDDPKQIRIVN"
BASE COUNT         2317 a         2926 c         2811 g         2384 t
ORIGIN      
        1 tagcgccgcc gatcacgctc gaagtgaaag cgcgtcgcgc gctcacgttc gtccgacgag
       61 agcatcttcc gcccagcgtc aagatcgacg ttcccatcat cgagccgcac cgcccaaaca
      121 tggacagtgt gattgagcgc cggaagttcg gcgggtgcgg ccggccaatt ggtcataaaa
      181 aagaatttac cggggtggat acgggatttg gattcgcaat ttcccccgaa aaggcgcaaa
      241 gactctaagt ccgaaatagt tgcgaccgaa gtgaaaatct ctcttacatt cttggattcc
      301 ttggctcgct aggacttggc tcggagtgac ctttgcgcct tcgcgcctcg gtgagagttc
      361 cccgatgagc tttgtatcta ttatattctc tgtggtcgaa tacgggggtc ttcacaccga
      421 aacttcgcgc ggattgcgtg cgccagtgac gattacacgt tcgccgtcgc ggatcacgcg
      481 cagatcgtac ggtccatcga aatatatcat cgtgcatcgt gcgcggatgt tcgtacactg
      541 tggtcttgtg cgcgatcact tcgacgtaaa tactggaaaa cttacccgct ggcagtctcc
      601 cgcaaacgct aagttcgaaa gctgtcattt cgaccgcagg gagaaatctt tgctagaatc
      661 ctcgatccga acgctttccg ttctttgtgg ctattgcgcc agcaacgtcg cggcatcgcg
      721 gatcgtcttg ccggatgaag tgcgcggcag cgaatcacgc ataacgatgc gatccggcac
      781 tttgaaattg gcgaggttcg cgcgcaaaaa tgtcagcaag tctttttcga ccaccgtcgc
      841 tcctgtccgc ggcacgacgg cggcgcctat cacctggcca cgcttggcat cggggacggc
      901 aaacacgatg gtctcctgca cgtccggata agtgtaaatt agattttcaa cctctgcggg
      961 atatacgttc aagccaccgg tgatgatcat gtcgtcgcgc cggccgagat ggtaaaaata
     1021 cccgtcggct tcgagcttgc cgacgtcgcc ggtgtaaagc caacctttgc ggatgcgttc
     1081 tgcggtagcg tcagtgcggc gaaaatagcc cttcatgaca tgggggccgc gcgtaatgat
     1141 ctctccagcc tcgcccggtg ccacgtcttt atcgctttcg tcgacgatgc gcacctccac
     1201 gcccggaacg gcccgaccga tgctgcccat cttggtttcg gccttatccg gatcgactag
     1261 ggtgatcatc ggcgaagtct ccgtcaaacc gtagccttga attactttga tatgcggctg
     1321 ggccgcttga aacgcacgca acagcggcat gggtatgctc gtgcccatca tcgcggcgat
     1381 tttcatgctg ctggtatcgt agttggccaa gtttttcacg cccagcaaaa gttgaaaaac
     1441 gatcgggaca ctttcaaaga cattgacccg gtatttttga atgccttcga gcaaggtgat
     1501 tgggttgatc tgatcgaaga tcaccagcgt atagcccttt tctgccaact cgttgcacac
     1561 cactggaccg acgatatgcg acatcggcag cacgcagccg cgtatggtcg tgacatcggt
     1621 gacgcccatt tcgcgcatca ctttggggta ctgcaccaga ttgccatagg acagaactac
     1681 ccctttgggc tcgccggtgg tacccgaggt gtaaagaatc aaagcatcgt cattggggtt
     1741 aaggttgggc ggcctagtta gtgctttcgc tgcactgaaa cgttgctcaa aactgcgctc
     1801 gtcgctcccg caggcgagcc aaaccgggat tggcgtcata gttttaaaga tctcttcgac
     1861 ttctggataa agctgttggt gcactacgag cagtttcaaa tcagcatcct tcaacacacc
     1921 ttcgagttct ttgcctttca atcgtgcgtc gaggatcacc gtaacggccc cgatcttctg
     1981 cgtcgaataa tacgccaacg gaatcgccat ggagttgggc aacagcacgc cgacgcggtc
     2041 gccttgtcgg ataccttcat cgatcaagtg gcccgccagc gcctgcacgc ggtggttgag
     2101 cgcggcaaag gttagctcct catctttggt gatgatcgcg gtcttgtccg gctggcgcgc
     2161 cgccgtccgg tccaaaactt ctccagcgta aaatcctact gaatcggtat cgtctgccat
     2221 aacgcaaaag ccctctgaaa tggtttatac cagcgcgata actgcgccaa atcgccgcgc
     2281 ttgagttcta atttgccctg ttgtgtcgcc aggaatgcat cgattctgtt gctgaacacc
     2341 gctcgccacc actcggcttt cccgctgatg agaaagtcgc cgtcggactc ttcggtcaag
     2401 gcggtgatct cgccgccgac aaattgcacg gtccacgccg cttcactatc ttccatgtgc
     2461 aaggtcaacg acgtggttaa gccggtagcg ctgccgaact ggcgaaaatc gccgtcacta
     2521 ttggcgctat ggaccagcgt ggccatatgt gctcgcgaga acaactcata agcggtagcg
     2581 gccgaggcgc tcgtttcgat cgactccgat ggctgcgaac cattgtgctt gcgggtaacg
     2641 ccgctgccgt tctgaaaaat ttggcaatgc accgcttcct cgagcagatc ggtatagaga
     2701 aagccttctt ggagagtctt accgatcgcc actgtaccat gacctttgat gatcgcgatg
     2761 ggattatatt taagcgcctc gaccaccggt tccgggtttg tcaccgtcgg cgtgtcctgg
     2821 ggaatgattg gcaccttgcc aatggtgtat ttctcttcat agctaagcgg cgcaaagctg
     2881 tcgtgcgtta aggaaaacgc cagcaccatc ggcggatgaa catggacgat cgcttgggca
     2941 tcggtaccgg catatactgc cagatgcatc ggcgtttcgc tcgaagctcg ctcacttttc
     3001 ttcaccggct tgccgtcggc atggatgcgc accaagtctt tcgcggccaa aaatcccagc
     3061 ggcgcatgct ggccggtgat aaggaaagca tttttaccga ggcggcaact cagattgcca
     3121 ccccaaccgc tgatcagccg gcgctcgaac agcagcttcc cccagcggat catgtcatcg
     3181 tcgattttag ccacagatca accttgttaa ctcttcaata gggcccatgg aatcgcttcc
     3241 gcactgagtc cgcggacggt tacaccggcc ggacaaatgg gctcgccaag aaaggttgac
     3301 acatctctgt cgatggttgg ttcattacgc acatcggcac taaaaagttc gatctgaacg
     3361 ccatgcaaac gtgccagttg cacagccaag agcgagccac aaatcccttg cgggccctcc
     3421 gccgcgaggc ggtcatagaa gaagcatagc ttggcaatct cgccatgagc gaacagtgtg
     3481 cccatcatat tgtcggaaat cagcgttgtc gggatgttgt gtttaagcag ttctggcgcg
     3541 atgtggcgtg cgccccaaag ctgcgggcgc atctccggaa tcaagaggct ccgggctccc
     3601 tccgctttca gcgcgctgag tagaaatgaa aattggctcg gcggcggcac accgtaaatc
     3661 aaaaggtatt tgccgcccgt tctcgtttcg atcacagaag aggctgtgtg agtgtggtat
     3721 ttcagccgaa aactttcggg cgtgtaaaca cctgcgaaac caatgagctg cgtgatgtac
     3781 tcagccggcg ttacgtcgaa cgatggatag cgcacaagca actgcgcgtg atccccggca
     3841 agtaaatccc ggccatgaaa tgtcaggagt tcgtcagccg gccgctcttc gatcggcaca
     3901 tcggcaccac gcggcaacga acgcggatct tgcacgagcg cgtaaaacgg cacgccgtat
     3961 tccttggcca tgcgcgcaat cggataggta ccgaccttgt taatgacatc agcgttttgc
     4021 gccacccggt cggcaccgac tatcaccgca ttggcttcac cttgcgccaa tgtgtgcgcc
     4081 actgcggagt cggggattaa aataacctcg acgccggcct gtgctagctc ccaagcggtc
     4141 aagcgcgcgc cttgcaaata gggacgggtt tcggtggcaa tgacggcaaa ctctttgccc
     4201 atctgtttgc aatacgctgc cacggcgacc agttcgccgc tgacattaca gtgtgtcaac
     4261 agccgcgccg gcgtgggcag aagcatcgcg gcgcgcttgg cccggccgct acggccgcgc
     4321 acgatttgct ggccgaactc gcgcgctttg gtcgcaatgg tcgcgccgac gtcggcgccg
     4381 gccgggagtt cgtcgaagaa gtcatcaacc atgatgccga gaccggcaaa atcgaaggtc
     4441 ggccgcgcgt cgcagaattg ctgtgttagt tgctgcaatt ggccccgcaa agacgccgct
     4501 tccgtacagt gataattttg tgcgagcagc gcggcgctat agagaaacgt caacacctgc
     4561 ccgaaggcgc gcgtcttcat ctgccggacg gcatcgagcg cctcggcgac atcagtcaca
     4621 tgaatgtagc gaatttgttg cgggacttgg atctcgtcga ggatctcaaa gccgtcgtca
     4681 tgccacaaaa ccggttcaaa cagcggactt gggacggcgc tcatgatatt agtgggcgtt
     4741 aaaggtattt cagcgcctgg cgcgcgactt cgatggtttt ctgctcagcc gcgaaaaacc
     4801 ccggatcgcg aaaaccgaat ttaccgtaga ggcattcatc cgataccgcc agtactgcgc
     4861 cggctttctt gccgcgcacc tgcgagatgg tcaaaaacgt cgccgtcacc atatcgatgc
     4921 tcgagacatt ttgttggttc aattgttgaa tcagttccgg cgtttcttga aacaatgcgt
     4981 cggtggtaaa tatccagcca agatgataac ggacctggca ctcttcggcc gcccgcttga
     5041 gtgcggcgat gatgccggcg tcggcgacgg tggaaaaatt ctcagcgacg taataacgcg
     5101 aagcgccgtc gccgcgcagg gcgccggtga cgatcaccaa atcgccgatt ttgacgctgt
     5161 cctgcagaga gccgcagctg ccaacgcgaa ttaacgactc ggcgcccgct ccgcagagaa
     5221 tctctgtcgt gatagccgtg tccggcgcga aacggccgcc attgccgatg ctgacgcgct
     5281 tgccgtcgag ccgaccggta tgcatctcgt agtcgagaaa ggtgaaactt ttttgccgat
     5341 cctccaatag cttcaacatc gcctcactgc gttctttggg acccggcacc agcgcatatg
     5401 gaccgagcga attcgccttg aggccaagca tagtgagcat gcgttgcggt gtgaagtgcg
     5461 attctttgat catgatcaca gcgccggcaa aataaagtcg agtaaatcct gggtcggcaa
     5521 ccctgcttcg gacaaattgt ggaacgagcg ctgacacagc gcgctcgaag tgagcattat
     5581 ttcggcgcca ctggcgagcg cttcgcgcat gcgctcggcg gcgttccact gcgccaattc
     5641 gggatcggcc tcaggcacac cgccgccgcc gccacaacac caagcccatc gttgattacg
     5701 ctccatctcg agcaccttaa tgccgggaat cgcctgaaga atatcgcgcg gcggcccgta
     5761 aacgccgcag ccgcggccaa ggtgacaaga gtcatggtag gtcaccctca tgtcgatcgg
     5821 tttggtgaag cgcagttgac cggatttgac cgcctgcgcg atgaactcga cgccatgcat
     5881 gacttcgagg ccattggtcc ctttggcgta ctcacgccag atgcgctggc aactgccaca
     5941 ggcgacgata actttgttga cgcctgcttg cgtgagcatc gataaattca gatctttcag
     6001 tcgttcgtat tcgcggcgaa aaccaagatc gaaagcgtac aatccgcagc atttttcctg
     6061 ctcgcccagt acggcaaaat cctcacccgc cagttgcatt aacttggcca atgcaatggc
     6121 gccgctcttg ccgctggcac gatcgctgga gcagccggcg aataacagcg tcgaagtttt
     6181 gtccgcgccg agttgcttga gacctagccc ttcggcccaa cccccgtgcg ctgtctgtcc
     6241 ccaaggattg tcctgttgtt taaggccatc gtaaatcgct cggtgagctg gcaaaggacc
     6301 gtctttttcg gtgatctctt cgcgcatggc gaccgtaata tcccacggcc cgtaaccatc
     6361 gccgcgcacg ccgcatagtt cctgacacat cagacaattt gtgcacgcgt atataacttc
     6421 ttttaattcc ggagtgatct gatcaagtcc atgaaacacg cgctgcgcca tcaaccaacg
     6481 cgacttcggc gtgtgtcgct gaaacttgta gtattcgtaa ggcggacatt tttctttcgg
     6541 ccccggcaca tcctccatcg gattggcggg cccatgcccg tagcaggaac cgcagagaac
     6601 gcaaccgctg ctgcgttctt cgagtccttt agcgtaaagc cactttttta cgcgttggag
     6661 cccgatcagt ttagggagtt gatcagccac gggagattgc gcctaaaaat agttaaagtt
     6721 taaactgtta taacttgcca ttattccgtg tcaatcgcgc gccgctcgtc cacctgggtt
     6781 actgaacgtt gactcagctt agggtgccga atcgacagtg aagaataaag actagtagac
     6841 gaggatggct agcgggatcg ggtcattccg cagcgggttc ttgatcgagc cgctttagca
     6901 aagctgacag atgtttttga tcgcctttgc tcaaacctgc gaaaatttca ccgacacact
     6961 tttcgaacag cggaatcgtg ctcttgtaaa gtttggcgcc tttatctgtt aacatcacat
     7021 ggacgacccg gcggtctttg tcatcccgct tgcggataac gaactttttg tcctctagcc
     7081 tgtcgacaat gccggtgaga ttgcttacgg tcaccaacat tttcgcgccg atctcgccaa
     7141 agggcaggct tccggcatac ccgatggtgg ccagaacata gaactgtggc ggtgtcaggc
     7201 ccaacaccaa caagtcgtct tcgagcttct tatgtgaacg taaactgaaa cgaagaaaac
     7261 gaacccaaag gctggtgcta tacgaggtaa aaaacggccg cttgacttct gtcacggcgc
     7321 taactttagt cggacactgg cgaaaaagca acttattgat cggtacgttg gttgatcgat
     7381 gtcaccgtcg accggcattg ccaacgtgcc agttgcgatg tagttaccac tagcggagtt
     7441 cacgaatatg aaactgcaca ttcttggctg cggcgacgca tttggcagcg gcgggcgcaa
     7501 tcagagtggc tatttggtcg aagctaaaga caagttattc ctgctcgact gcggaccgac
     7561 aactttgctg tcacttaaac gcgcgggctt tgatccgcgc gccattgaag caattctctt
     7621 gagtcattta cacggcgatc atttcggcgg aattgcgttt ttctttctcg agtatctgta
     7681 tcttcagcca cgattagagc cacttgtcat cgccggaccc ccaggcaccg aagccaaggt
     7741 tcgcgcgctg ttcgacgtgt tgtacggcgg cggaactgat cgggagattc caccgacccg
     7801 ctttgtggtt ttgtggccgg acgagccggc ggacatcaac ggaatcgtca tcgaaccctt
     7861 ccgcgtgacg caccaaagcg aggctatctc gttaggctta aaagtcagtt acgaggcgaa
     7921 aataatattg ttttccggcg attcgatatg gactgaacac tttgttgaac tagccgcggg
     7981 cgtcgatctt tttctttgcg agtgctcatt ttttcgcgag caacccggga tgcacgtaaa
     8041 ttatcaggat gtggccgcca acctcggccg cctgcgctgc aagaagttga tcctcaccca
     8101 tctgggtgaa gaaatgctgt cgcgtaggag tgacatttcc gccgtcattg ccgaggatgg
     8161 aatggtgctg gaggtctaaa aatggatcgt gatcagcttg cttcccgcat cgccggaatc
     8221 tgcagaatta gcgagcggta gccgttttta gcgaggatga cttgagcgac ttcctgagca
     8281 gcgatcagtt cggtgtaagg accgacgtgg acacgataag cggcttcacc gcggccgaca
     8341 gccccagtat cgatcaccgg ttcgaatccc atttcactaa tttggcgtga aactaactcg
     8401 gcgcgttcgc gttcacggaa cgctccaact tgaagaatat aaccggtcag caattttgac
     8461 cgttggttat cgccgggtaa aatcaccgtt gagacacgct ggcgctcagc agcttcagtg
     8521 gaaagcagct tttgagtgct atgcgccgat ttcaaagtgt cgaaattatc gaaaccgtat
     8581 tcgagcagat gtttagcatc gctccacata ttggtcgcgc ccaaaatcga cacaatcagc
     8641 gttacgccgt cgcgttgcac cgcaccgaca taacagcgtt gcgccgcatg ggtatagccg
     8701 gttttgccac ccaaggcccc gtcgaaatcc cataacagcc ggttatgatt gcgcaccgag
     8761 atgcgccgcg gtacgacttt ctttttaacc actgccgtcg agctcaccga actgaacttg
     8821 gtgtggatga tctcgcgaaa cagcgggatc ttcatcgcat agcgaaagat cgttgctaga
     8881 tcgcgcactg tcgaataatg atcgggagca gtcagtccgt gcggattaat aaaatggctg
     8941 tttgtggcgc ccaaactttg ggcttttttg gtcatgagat ccgtgtaacg ttccaccgaa
     9001 ccgctgatac cctcggccaa cacgacgctg gcgtcgttgg ccgaggcgag cataattccg
     9061 taaaggagtt cttcgatcga gaccgtctgg cctggccgta aatacagttt ggtaggcgga
     9121 acgcgcgtgg catctttaga tacagttaag ctttcattaa gcttcctgcc gctctccagg
     9181 gctaaaatag ccgtcatcac tttagttgtg cttgcggggg gtaaacgcag gtccgcatct
     9241 ttctgaaaca aaatccgccc agtggcggca tccatcaaca aggcggcctt tgcggtgatt
     9301 tcatcatccg gagaaaacga ccagagggaa gaaaccgaag ttaaaacaac gagaatcgcc
     9361 aagctgatcg cggcagtaaa tagtcgacgg tagcgcagaa gctgaacctc ctaaccgcta
     9421 attagacgca aggatacagg aaaagccgga actgaagaaa cagtttcgtt ttataacacg
     9481 tcttcgatag ggagaacaat aaaaaagcgg cgctccctgc ttaaatattt gggactggtg
     9541 aacgcaatgg ccggacttga gtcatgctga cccaaatttg cagcacaacc gattcaagtt
     9601 tttttcgggg cgattccctg acgggaaatc tcccgttcgg cgcgctcggc tatgccggcc
     9661 tgctctccta gagcctcacg gcgaatccgc tcctggcaag gttggcagat gccgccctgc
     9721 aagacctcgg aagtctttgc gcatatcatg catcgtttat tggcatcggc cataaaatac
     9781 cccgaatctt tccttgatcg gcaatctctc tcgcgcgcta atttacaatc ctaatctgct
     9841 ttggatcatc gaggactagt tccaacccat actgtgggtg atctttgatc tgaccggtga
     9901 tttccacatc cttattctcg aaactcttcg gcaaaagctg gctcttttcg aataactcga
     9961 ctgctggcga aaaaattacc gcggtaagtg cctctcggga cggaccgaaa tttagaaagt
    10021 aggtattact cttggcagaa tggccgacgc gaaacacgcg gccgcgcacg cgcgcttgct
    10081 tgccggtaag cgcgcgaagt ttctctacat ctcttgctac caatgccgga agtgcggcag
    10141 cctgctccgg ttctgcggct gatggcgcca aaggcatgta gtttactcga tgcgcttggc
    10201 ctggcatcga agcggactcc tcaggcgctt ttgatgacga aactctcacc ggcagcgggt
    10261 tccaccgtag atacaacacg tgggtgaaga agccggcacc gaagatcagc aagctcaccc
    10321 aaatctttac agcggcgcga gccattacgg cctcctttcc cacagccgca tgaaggcctg
    10381 gcgatactca tcggcgagag gaccggcctc gcgaaaaagc agaagatttt cgtcgttc
//
LOCUS       DATLCH010000064         9674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_211070, whole genome shotgun sequence.
ACCESSION   DATLCH010000064 DATLCH010000000
VERSION     DATLCH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211070"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VMZ02_01835"
     CDS             <1..1014
                     /locus_tag="VMZ02_01835"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70696.1"
                     /translation="KRIGWLLVAALSCIGGLDTAGAQTKRKIVFGVPVTPPNVVHLPP
                     YIAKEMGYFNDLNIDVELVTFEGGTQTLRGSVAGGLDITGTSSDPAIIAAARNAGTKV
                     LGTYSHKLSQSMLVQGDIKNCKDLKGRKIGIQEVGAFNEVMSRAVMASCGLTPKDVEY
                     VPVSTKGRVAGLLSGQIDTAILHVDQAIVAKKKKADLNILVNLWEPLPKWLYAAYIAP
                     EKEIKENRQLYIDLMTALVKANRFIYNNKAKVVEIGVKYTQQDADAVAQTYDILAAVG
                     AWPVNDGLPKDMVEWTINRQVELGTIKAQEKPSYEKLVDISIVEAAIAKAGGRLKGDK
                     RWD"
     gene            1102..1398
                     /locus_tag="VMZ02_01840"
     CDS             1102..1398
                     /locus_tag="VMZ02_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HUR70697.1"
                     /translation="MANCNTEPPFTDPVVIPIEDHLDLHPFQPKEIPSVVEEYLHECK
                     EAGFYQVRLIHGKGKGVQRNIVRALLEKHADVESFHDAPLETGSWGATIVNLKR"
     gene            complement(1395..1898)
                     /gene="lspA"
                     /locus_tag="VMZ02_01845"
     CDS             complement(1395..1898)
                     /gene="lspA"
                     /locus_tag="VMZ02_01845"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HUR70698.1"
                     /translation="MSAKWRIVGGLVAAIVVLDQFTKIIVQRTMELHQSIPIVDGLFN
                     LTYVRNTGAAFGIFAGSAEIFRRPFLILVSILASAFIISLLRRLPSDEKGLITALTFI
                     LGGAIGNLIDRVFYGEVVDFLDFYWRSYHWPAFNIADSFITVGVTIALYCLYRHEGPD
                     PFTRAKS"
     gene            complement(1895..4699)
                     /gene="ileS"
                     /locus_tag="VMZ02_01850"
     CDS             complement(1895..4699)
                     /gene="ileS"
                     /locus_tag="VMZ02_01850"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HUR70699.1"
                     /translation="MDYKDTLNLPKTDFPMRANLPQNEPKQVAKWDEERAYFQILQAN
                     EGKPKFILHDGPPYANGNIHIGHALNKILKDIIIKYKAMTGHSAPYVPGWDCHGLPIE
                     LQVEKNVGRAKKLAMSKAEIRKLCKNYAEKYISIQREEFKRLGVLGDWENPYRTLEPH
                     YEAQEVRELGKFVASGALYRKKKPVYWCASCVTALAEAEVEYEEHTSTSVYVKFAVTD
                     PKGLFAVEPVHGTFFVIWTTTPWTLPANQAIAVHPKFTYRRVKTAHGELIINQELVES
                     VMNAVGLHAKDYEVLPEGWTGAELEGIVCRHPWLDRGSAIVLGDYVTQDQGTGAVHIA
                     PGHGQEDYEVGMRYGLAVMAPVDPEGRFTAEAGDLQGESVFKADPRIIQKLIDHHALL
                     KEDKLTHSYPHCWRCKKPVIFRATEQWFISMEKNDLRNQALRAIDEVKWIPPWGRERI
                     RGMLEARPDWCISRQRTWGVPIPAVYCKKCNQALLTREICDHVAAIFAKEGSDAWFTR
                     PVSELMPTGLKCPHCGAQDFSREEDILDVWFDSGVSYAAVVEQDPRLGGRADLYLEGS
                     DQHRGWFHTALLTSLATRDRAPYASVLTHGFTLDGKGRKMSKSLGNTIAPQDIAKKFG
                     AEILRLWVSAEDYREDVRISDEILNRLVEAYRRIRNTARFLISNLYDFDPAKDRVPAD
                     QLDELDSWILQRTENLLGRCRDAYEKCEFHVVYHSVNNFCAVDLSAQYLDIVKDRLYC
                     EATNSRERRAAQTTLYRILEVLVHVTAPILSFTADEIWSYLPGKANRPQSVFLSSIPE
                     PDTGAAASILGEKWDRIFAERSLVLKALEQARTAGEIGHSLDAKVVLHDSDPHRSALH
                     QLMTADSQKLADVLIVSQVALDATQAPDDPKDLRVSVSKADGVKCARCWKYDIKIGCD
                     DKHPDVCPRCARVLSAGAAA"
     gene            complement(4755..5132)
                     /locus_tag="VMZ02_01855"
     CDS             complement(4755..5132)
                     /locus_tag="VMZ02_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70700.1"
                     /translation="MRMILPPIKERRAIDRLLSAFFNQCKASDFKKAISLLCRFYHLK
                     QPKIEWFEYIDWGKTAGKTYETGQIYLVHPENWKKGRKYNSARRWINLVYHEMGHYVF
                     WADAENKADMFATRMVRGLNSQK"
     gene            5385..6320
                     /locus_tag="VMZ02_01860"
     CDS             5385..6320
                     /locus_tag="VMZ02_01860"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70701.1"
                     /translation="MIAWLSEKLAYLGTWFGFKIGCAAIRLCPQAVFRLADGLAKIGY
                     YLFRGFRTRSIANIQLALGDRLSAREIDTVARQSLRNFLLACVEIGVAVESSDAKIKA
                     DIPVVGRKHLDAALAKGKGVLLLSAHLGNFFLVGCRIAVDGLPAFVLVNQPRDGRFAD
                     LMDDYRREARQNTIHARPRQRALREITQVLRQNQIAVVIADEYRRGSGIEVSLFGKTV
                     IARRGPVSLALRTGAAVVPACMVRQADDSLRLVIEPELELKRDERDAESIRENTARIT
                     RWLERTVRRYPEQWNWMNLRWWAEGASEERHQSNN"
     gene            6350..7096
                     /locus_tag="VMZ02_01865"
     CDS             6350..7096
                     /locus_tag="VMZ02_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR70702.1"
                     /translation="MKISKKATDELKTKVALGNRIMFHQGLADYHGHVSARIPGTRKF
                     FIKPVLAPLGEVASKHIIEVDIDQYFEICEANYAKAGNKRAVTKIKNPPRETMIHAAI
                     YNARPDVNSVVHTHQTIATAFSVAGTPILPIYNQAAVFAPETPIFPSPRLIYTMRDGR
                     EICATLQDRMAMLLKGHGIIVCGDSLEYATVHAIYLERTAYMQFIASCVGKPTVMPQA
                     EIDYMKENMMFRSYDAFAYFRAQLPKGARL"
     gene            7135..8724
                     /locus_tag="VMZ02_01870"
     CDS             7135..8724
                     /locus_tag="VMZ02_01870"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR70703.1"
                     /translation="MAKREEKVTDSGKKPIQDLREWLDRVEALGDLVRVKDAVSCEEE
                     MSAIGYLVAKQKPSPAILFDNIKGYEKSPYKAQSLWNILGPSIPRIALTMEEPHDTST
                     VELIRRTKDKLKNRMAPREVSAAEAPMYQNTLTGDKIDLTQLPIPRHWPLDGGRYAGT
                     ADCVITRDPDSGYLNVGTYRMMLQGKNECGLYLSPGKDARLHITRSWQQGKPVEVAAA
                     WGIDPLFMVLGSQTFPKNVSEYEYLGGIKGEPIPVVKGKSTNLLLPANAEFMIEGVIK
                     PNSVRSEGPFGEFPGYYGRPEAGCPLVEITAIHYRNNPILTNALMADYPSNEQSGFFS
                     VIRSARIWDDLDKLGIPGIQGVYAHPAGAGGFGMTVIALEQRYAGHAAQALALAAQVP
                     GGAYYTKWIIAVDEDIDPTDMDQVIWAMSSRCNPIDDVDILRNTWSTWLDPTQNPPEK
                     RPYGSKALINACKEHRYLPVFSKRTALRKEMYDKVAAEWKKLGLPGQVPEVRAFEEKK
                     DVVYHEVGGFEPGKQPGEDKN"
     gene            complement(8847..9245)
                     /locus_tag="VMZ02_01875"
     CDS             complement(8847..9245)
                     /locus_tag="VMZ02_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HUR70704.1"
                     /translation="MATRLARVAIPQKNLTGDFMKKIEAIIKPFKLDEVKQKLTSVGI
                     TGMTITEVKGFGRQKGHTELYRGAEYTVDFLPKVKIEILAPEEMTPKIVEAILESAQT
                     GKIGDGKIFVSTVEEVIRIRTNERGEEAIS"
     gene            complement(9262..>9674)
                     /gene="glnA"
                     /locus_tag="VMZ02_01880"
     CDS             complement(9262..>9674)
                     /gene="glnA"
                     /locus_tag="VMZ02_01880"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HUR70705.1"
                     /translation="SARNRSACVRIPMYSTSEKARRLEFRTPDPSANPYLAFPAMMMA
                     GLDGIKNKIEPPKPIDKNLYELEPEEAAKVKSMPGSLDQALDALEKDHAFLLTGDVFT
                     KDVIETWLDYKRKHEVDAIRLRPHPWEFALYFDI"
BASE COUNT         2245 a         2535 c         2689 g         2205 t
ORIGIN      
        1 aaaagaatcg gttggttact cgttgccgcg cttagttgta ttggaggatt ggatactgcc
       61 ggggcacaga ccaagcgcaa gatcgtattc ggagtgccag tgacaccgcc caacgtcgtt
      121 catctgccgc cgtatatcgc caaggagatg ggatatttta acgatctgaa tattgacgtc
      181 gaattggtca ccttcgaagg cggcacgcag actttacgcg ggagcgttgc cggcgggctc
      241 gatattaccg gtacaagttc ggatccggcg atcattgctg cggcacgcaa cgccggcacc
      301 aaagtgctcg gcacctattc gcataagctt tcccaatcga tgctagtgca aggcgacatc
      361 aaaaattgca aggatctcaa aggccgaaag atcggcatcc aagaagtggg cgcgtttaac
      421 gaggtcatga gccgagcggt catggcgagt tgcggcttga cgccgaagga cgtggaatac
      481 gtcccggtga gcactaaagg cagggttgct ggactgctca gcggtcaaat cgacacagcg
      541 attctgcatg tcgatcaagc aatcgtcgcc aaaaagaaaa aagccgacct gaatattctc
      601 gtcaatctgt gggagccgct gcctaaatgg ctttacgccg cttacatcgc accggaaaaa
      661 gagatcaaag aaaaccgcca gctttacatc gatctgatga ccgccctagt caaagccaat
      721 cgctttatct acaacaataa agcgaaggtt gtggagatcg gcgtgaaata cacacaacag
      781 gacgccgacg cggtagcgca gacttacgat attcttgctg ctgtcggcgc gtggccggtc
      841 aatgatggct tgccgaagga catggttgaa tggacgatta accgtcaagt ggagctcggc
      901 accatcaaag cgcaggagaa accgagctac gaaaagctcg ttgacatctc gatcgtcgaa
      961 gccgccatcg ccaaagcggg cggtcggtta aaaggtgata aacgctggga ctgaagtcat
     1021 cagacaaaat acaaaggagc ttggaggaca gagcgctcct tgtggcatgt ctttcagcga
     1081 tccagggaaa atcacgcaga gatggccaac tgcaataccg aaccgccatt taccgacccc
     1141 gtggttattc ccattgaaga tcacctcgat cttcatccgt ttcagcccaa agaaattccc
     1201 agcgtcgtcg aagaatatct tcatgaatgc aaagaagctg gcttttacca ggtgcgtttg
     1261 attcacggca aaggcaaagg cgtgcaacgt aatatcgtgc gggcattgct ggagaagcat
     1321 gccgacgtcg aatcgtttca cgatgcgcca ctcgagaccg gcagctgggg cgctacgatc
     1381 gttaatctga aacgctaact ttttgcgcga gtaaatggat ccggcccttc gtgccggtag
     1441 aggcagtaaa gcgcgatggt gacgccgacg gtgatgaagc tgtcggcgat gttgaatgcc
     1501 ggccagtggt agctacgcca ataaaaatcc agaaagtcga cgacctcgcc atagaaaacg
     1561 cgatcgataa gattgccgat cgccccaccg aggataaacg taagtgcagt gatcagtccc
     1621 ttttcatcag acggcagccg tctgagtagg ctgataataa aggcgctggc caggatagac
     1681 accaaaatta ggaaaggccg gcggaaaatt tcggcgctgc cggcgaagat tccgaaggcg
     1741 gccccggtgt tgcgcacgta cgtgaggtta aatagcccgt caacgatggg aatcgactgg
     1801 tgcagctcca tcgtccgctg cacgatgatc ttcgtaaact gatcgagaac gacaatggca
     1861 gctaccaggc cgccaacaat tctccacttt gcgctcaagc cgctgctccg gcactcaaga
     1921 cgcgagcgca acgagggcag acgtccggat gtttatcgtc gcagccaatt tttatatcgt
     1981 atttccagca gcgtgcgcat ttaacgccgt cggctttgct gactgaaact cgcaaatcct
     2041 tcggatcatc aggtgcttgg gtcgcgtcaa gcgcaacctg ggaaacaata agaacatctg
     2101 ccagcttttg agaatcggca gtcatcagtt ggtgaagtgc agatcgatgt ggatcgctat
     2161 catgaagaac gacctttgca tccaaggaat ggccgatttc cccggctgtc cgtgcttgtt
     2221 ccagggcttt taggacgagg ctacgctcgg caaaaatccg atcccacttc tccccgagta
     2281 tagaagcagc agcgccggtg tccggctccg gtatcgatga aagaaaaacg ctctgtggtc
     2341 gattggcttt tcccggtagg taactccaga tttcgtcggc ggtaaacgat agaatcggcg
     2401 cggtcacgtg aacgagcacc tcaaggattc tataaagagt cgtctgagcc gcgcgtcgtt
     2461 ctcgggaatt tgttgcctcg cagtatagtc ggtctttgac gatgtccaag tactgcgcgc
     2521 tcagatcgac agcgcagaag ttattgacgc tgtgataaac gacgtggaac tcgcattttt
     2581 catacgcgtc gcggcagcgg ccgagcaagt tttccgtgcg ctgaagaatc cagctgtcga
     2641 gttcgtcgag ctggtccgcc ggcacgcgat ccttcgctgg atcgaagtcg taaaggttgc
     2701 tgatcaagaa gcgcgctgtg ttgcgtatgc gccggtaggc ttcgaccagt cgattgagaa
     2761 tttcatctga gatgcgcacg tcttcgcggt aatcctcggc ggaaacccac agtcggagga
     2821 tttcagcgcc gaactttttt gcgatgtctt gcggcgcgat ggtattgccc aaagatttgg
     2881 acattttgcg gcccttgcca tcgagtgtga aaccgtgggt caacacactg gcgtagggcg
     2941 cgcgatcgcg ggtcgctagg gacgtcaaca gggcagtatg aaaccagccg cggtgttgat
     3001 cgctaccttc gagataaaga tcggcgcggc cgccgagacg cgggtcttgt tcgaccacgg
     3061 cggcatagct gacgcccgaa tcgaaccaaa catcgagaat atcttcttcg cgggaaaaat
     3121 cctgagcgcc gcagtgagga cactttaggc ccgtcggcat cagttcgcta accggccggg
     3181 taaaccaagc atccgagccc tcttttgcaa agattgccgc gacgtgatca caaatctctc
     3241 tggtcagcag tgcttgatta cacttcttgc aatagaccgc cgggatgggc acgccccagg
     3301 tgcgctggcg cgagatgcac caatccgggc gcgcttcgag catgccgcga atgcgctcac
     3361 gcccccaagg cggaatccat ttgacctcgt cgattgcccg cagcgcctgg ttgcgcaagt
     3421 cgtttttctc catggagata aaccactgct ccgtggcgcg aaagatcacc ggctttttgc
     3481 agcgccaaca gtgcgggtag ctatgggtca gcttatcttc tttgagcaga gcatggtggt
     3541 cgattaactt ttgaatgatg cgcgggtcgg ctttgaacac cgactcgcct tgtaaatcgc
     3601 ccgcttcggc agtgaagcga ccctcagggt cgaccggcgc catcactgct aaaccgtagc
     3661 gcatgccgac ttcgtagtct tcttggccat ggcccggggc gatatggacg gcgccagtgc
     3721 cttgatcctg ggtcacgtag tcgcccagca caatcgccga accgcgatcg agccacggat
     3781 ggcgacagac gatgccttcg agttcggcgc cagtccaacc ttcgggaaga acttcatagt
     3841 ccttggcgtg caacccgaca gcgttcatta cgctctcgac caactcttgg ttgatgatca
     3901 attcgccgtg agccgttttc actcgtcgat aggtgaattt gggatgcacg gcgatcgcct
     3961 ggttcgccgg caaagtccac ggggtagttg tccaaataac aaaaaaggtt ccatgtaccg
     4021 gctcgacggc aaacaaaccc ttcggatccg tcactgcgaa ttttacgtac accgatgtcg
     4081 acgtgtgctc ttcgtactcc acttcggctt cggccaacgc tgtcacacat gaggcgcacc
     4141 agtaaaccgg cttctttttg cggtagagcg cgccggacgc aacgaatttg cccaattcac
     4201 gcacttcctg cgcttcgtag tgtggttcca acgtgcgata aggattctcc caatcgccga
     4261 gcaccccgag gcgcttgaac tcttcgcgtt gaatcgaaat gtacttttcc gcgtagtttt
     4321 tgcagagttt gcgaatttcg gctttgctca tggccaattt tttggcacgg ccgacgttct
     4381 tttccacttg cagttcgatc ggcaagccgt ggcaatccca gcctggcaca tacggcgccg
     4441 aatgaccggt catcgctttg tacttgatga tgatgtcttt gagtatttta tttaacgcat
     4501 gaccgatatg gatattgcca ttggcgtagg gcggtccgtc atgcaaaatg aacttaggct
     4561 tcccctcatt tgcctggaga atttggaagt aagcccgctc ttcgtcccat tttgcgacct
     4621 gtttcggttc gttctgcggc aaattagcgc gcatgggaaa gtcggtcttg ggtaaattga
     4681 gggtgtcttt ataatccatg aaaacctaaa ttctgcggcg cccgtgggtt gtggcgggtc
     4741 gtgatcgcag tcttttattt ttggctgttt aagccgcgaa ccatgcgcgt ggcgaacata
     4801 tcggctttat tttccgcgtc ggcccagaaa acgtagtggc ccatttcatg ataaacgaga
     4861 ttgatccagc gccgtgcgct attgtatttg cgccctttct tccaattctc cggatgaacc
     4921 aagtaaatct gtccggtctc gtaagtcttc cccgcggttt tgccccaatc gatatactcg
     4981 aaccattcta ttttaggctg cttgaggtga taaaaccggc acagcaggct gatggctttt
     5041 ttaaagtcag aagctttgca ttggttgaaa aacgccgata ataaacggtc gatcgcccgc
     5101 cgttccttta tcggtggtag aatcattcgc ataaatcttg tccgggttca cagcaatcgg
     5161 aaattattaa catacctgtc ggttaatagg aactgcccta gcgtggcgct ggagttgacg
     5221 tgtttctgta acacattgaa atcgtgtaac tttgctcagg aaagttggac agggcagagc
     5281 accggcatgg taccttggga cggttgcctc gtagaaaaaa ctagcgtaga ttggcgtact
     5341 ttaatgcatg gcagagcggt gcgaaccgta gctgtcgata attgatgatc gcttggctct
     5401 cggaaaaact agcctaccta ggtacttggt tcgggttcaa aattggttgc gcggcaatcc
     5461 ggttatgtcc acaagctgtt tttcgactgg cagatggact agccaagatc ggttactact
     5521 tatttcgcgg atttcgcacc cgttccattg cgaatatcca gcttgcgtta ggcgatcgcc
     5581 ttagcgcgcg agagatcgac accgttgcgc gccaatcgct gcgaaatttt ttgctggctt
     5641 gtgtagagat cggcgtcgcg gtggaatctt ccgacgccaa aatcaaagcg gacattccag
     5701 ttgtcggccg caaacatctc gatgcagcac tcgccaaagg caaaggcgtg ctgctgctca
     5761 gcgcgcattt gggaaatttt tttctcgtcg gctgccgtat tgcggtggac ggtttgccgg
     5821 ctttcgttct ggtcaaccaa ccgcgcgacg gccgttttgc cgatttgatg gatgactatc
     5881 gccgcgaagc tcggcaaaac acgatacacg cgcggccgcg tcagcgggcg ttacgggaga
     5941 tcacccaggt actgcggcaa aatcaaatcg cggtcgtcat tgccgacgaa tatcgcaggg
     6001 gctctggcat tgaagtctcc ctatttggta agacagtgat cgctcgtcgc ggaccggtga
     6061 gtctggcgtt gcgtactgga gctgcggtgg ttcccgcttg tatggtgcgt caggctgacg
     6121 acagtttgcg gctcgtcata gaaccggagt tggagctgaa gcgagatgaa cgcgacgccg
     6181 agtcgattcg tgaaaacacg gcaagaatca cccgctggct cgaacgtacc gtgcgccgct
     6241 atcctgagca atggaactgg atgaatttgc gctggtgggc ggagggcgcg tcagaagagc
     6301 gccatcaaag taataattaa cggtattgta gtgagaaagg ggaccgaggg tgaaaatcag
     6361 caagaaagct accgacgagc taaaaactaa agtggccttg ggcaatcgca ttatgttcca
     6421 ccaaggtttg gccgactatc atggtcatgt cagcgcacgc attcccggca cgagaaaatt
     6481 tttcatcaaa ccggtgttag caccgctcgg tgaagtggcg tccaagcaca ttatcgaggt
     6541 cgacatcgat cagtatttcg agatctgcga ggcaaactac gccaaggccg gcaataaacg
     6601 cgccgtcacg aagatcaaaa atccgccgcg ggaaacgatg attcacgccg cgatatataa
     6661 tgcccgaccg gacgtgaact ccgtcgttca tacccatcag acgatcgcga cggcgttttc
     6721 cgtcgccggt acgccgattc tgccgatcta taatcaagcg gcagtgttcg ctcctgaaac
     6781 gccgattttc ccgagcccta gactgattta taccatgcgc gacggtcgag agatttgcgc
     6841 cacattacag gaccgcatgg cgatgttact caagggtcat ggtattattg tctgtggcga
     6901 cagcttggaa tatgctacgg tgcacgcgat ttatctggag cgcaccgctt acatgcagtt
     6961 catcgccagc tgcgtcggca agccgacggt catgccccag gcggagatcg attacatgaa
     7021 agaaaatatg atgttcaggt cgtatgatgc cttcgcgtat tttcgcgcgc aattgccaaa
     7081 aggcgctagg ctgtaaggcc caaccggtga gtgaaaactt tggaggataa ctcgatggct
     7141 aaacgagaag aaaaagtaac cgatagcggc aaaaaaccga ttcaagatct gcgcgagtgg
     7201 ttggatcgcg ttgaagcttt gggcgatttg gtgcgggtta aagatgcggt gagctgcgag
     7261 gaggagatga gtgccatcgg ttatttggtc gccaaacaga aaccgtcgcc ggcaatcctg
     7321 ttcgataaca tcaagggata tgaaaagagc ccgtacaagg cgcagtcgct gtggaatatt
     7381 ctagggccga gcatcccgcg tatcgcctta accatggaag aaccccacga tacctcgacc
     7441 gtggaattga ttcgccgcac caaagacaaa ctgaaaaatc gcatggcgcc gcgggaagtt
     7501 tccgcggctg aagcgccgat gtatcagaac accctgaccg gggataagat cgatctgacg
     7561 cagctgccga tcccgcggca ttggccgctt gacggcggcc gttacgccgg caccgcggat
     7621 tgtgtcatca cgcgggatcc cgacagcgga tatcttaacg tcggtactta ccgcatgatg
     7681 ctgcaaggga agaacgaatg cggtctctac ctgtcgcctg gcaaggatgc acgtttacac
     7741 atcacccggt cgtggcagca gggtaagcct gtggaagtcg cggcagcgtg gggcatcgat
     7801 ccattgttta tggtgctggg ctcgcagacg tttcctaaga atgtatctga atacgagtat
     7861 ttgggtggca tcaagggcga acctattccg gtggtcaagg gaaagtcgac gaatctattg
     7921 ctgccagcca acgccgagtt catgatcgaa ggcgtcatta aaccgaactc tgttagaagt
     7981 gaaggtccct ttggtgaatt tcccggttat tacggccgcc ccgaagccgg ctgcccactg
     8041 gttgaaatta ccgcaatcca ctatcgcaac aatccgatac tgacgaacgc cttaatggcg
     8101 gattatccgt ccaatgagca gagtggattt ttttcggtta ttcgctcggc acgcatctgg
     8161 gacgacctcg acaagctcgg cataccaggc attcaaggcg tctatgctca cccggcgggc
     8221 gctggcggtt tcggtatgac ggtgattgcc cttgaacagc gttacgccgg ccatgcggcc
     8281 caggcgctcg ctttggcagc ccaggttccc ggtggtgctt attataccaa gtggatcatc
     8341 gctgtggatg aagatatcga tccaacggat atggaccaag tgatctgggc gatgagcagc
     8401 cgctgcaatc ccatcgacga cgtcgatatc ttacgcaaca cctggtctac ctggctcgac
     8461 ccgacgcaga atcccccgga aaaacgtccc tatggttcta aagcgttgat caatgcctgc
     8521 aaagaacacc ggtacctccc ggtgttctcg aaacgaaccg cgctgcgcaa agaaatgtac
     8581 gataaggttg ccgccgaatg gaagaaactg ggtctacccg gccaggttcc agaggtgcgg
     8641 gcgttcgagg aaaagaaaga cgtggtctac cacgaggtcg gcggctttga gccgggcaag
     8701 cagccaggag aagataagaa ctagtttacc ccactagcgt cgcaagtttt ttggccgagc
     8761 aatgcggcgc actacgttag atcccattgc tggcgaacag cttcggccac atcatgccag
     8821 gccgaggttg ctgccaggcg aaaatgctaa ctgatcgctt cttcgccgcg ttcgttggtg
     8881 cggatgcgga ttacttcttc gactgtcgag acaaagatct tgccatcgcc aatcttgccg
     8941 gtttgggccg actcgagaat agcctcgacg attttcggag tcatctcttc gggagctagg
     9001 atctcgatct tcaccttggg caagaagtcg accgtatact cggcaccacg gtaaagctcg
     9061 gtatgtcctt tctgccgacc gaagcctttg acctcggtaa tcgtcatgcc ggtgatgccg
     9121 accgaggtca gtttttgctt aacctcatcg agcttgaaag gtttgatgat cgcttcaatt
     9181 tttttcataa aatcccccgt aagatttttc tggggtatgg ctacccgcgc aaggcgggta
     9241 gccatagagt cagtttaaca cttagatatc gaaatacaga gcgaactccc aaggatgggg
     9301 gcgcaaccgg atggcatcga cttcatgctt gcgtttgtag tcgagccagg tctcgatcac
     9361 gtccttggta aacacgtcgc cggtgagcaa gaaggcatgg tccttctcca gagcgtcgag
     9421 cgcctgatcc aacgatcccg gcatcgattt caccttggcc gcctcctcgg gctccaactc
     9481 atacaagttc ttatcgatcg gtttgggcgg ctcgatcttg ttcttgatgc cgtcaagccc
     9541 cgccatcatc atcgccggaa aggcgagata cggattggcg ctggggtcag gagtgcgaaa
     9601 ctccagccgg cgggccttct cgctcgtcga gtacatggga atacgcacgc acgcgcttct
     9661 gtttctggcc gagt
//
LOCUS       DATLCH010000065         6263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1708941, whole genome shotgun sequence.
ACCESSION   DATLCH010000065 DATLCH010000000
VERSION     DATLCH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708941"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1056)
                     /gene="hemC"
                     /locus_tag="VMZ02_01885"
     CDS             complement(139..1056)
                     /gene="hemC"
                     /locus_tag="VMZ02_01885"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HUR70706.1"
                     /translation="MNRVRIGSRGSALALAQSNWVKQQIEAHYPDLQVELSIIKTSGD
                     RFSDRPISMLGGKGVFTKEIEDALLNQTIDLAVHSMKDLPTELPAGLTIAAVPVREDA
                     RDVLVSRNGLKIADLAPSARIATGSLRRQAQLLHHRGDLVVVPIRGNVDTRLRKLDEG
                     EADALIMAAAGLIRIGRAERIGEILPDEICVSAVAQGALAIETRGDATVRQLLSFLHN
                     STSEAETRAERALLRSLSGGCHVPIGARARVDGNALNMIAVVASPDGATLCRAQLTGE
                     AQEGEQLGACLADELRRQGADKILARVDS"
     gene            complement(1053..2342)
                     /gene="hemA"
                     /locus_tag="VMZ02_01890"
     CDS             complement(1053..2342)
                     /gene="hemA"
                     /locus_tag="VMZ02_01890"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HUR70707.1"
                     /translation="MRKEIVIVGLNHRSAPIEVRESVAFESAYLHDALTRLRNYSSID
                     EGVILSTCNRVEVVAAASDSTTAYSEIGRFLSEQKAQRHGIALTEHTYQYRGVEAIRH
                     LFRVAASLDSMVVGEPQILGQLKQHYDAAQQAGSVGTILHRLFHRSFSVAKRVRSETG
                     IGSGAVSLSSVAVDLAKRIFDRFDDKTVMLIGAGKMGDLMARHLQSHGVRSLMVTNRT
                     FERAVELAERIHGNPIRFEDFPQYLKMADLVIGCTGAPEVLVNAAQVDRVLRERKHKA
                     MFFIDIGDRRNFDGKINDLDNVYLYNIDDLRNVAEENLQGRSNEAAKAEGIVSEEVQS
                     FVRWIGSLEQVPTITALRQRFDDIRRGEIDKSLGGVLKTLSPEQRAALEDMTTAMINK
                     FLHAPITELKRRGDRDNDDEAALYLAALKKLFDLEGK"
     gene            complement(2346..3164)
                     /locus_tag="VMZ02_01895"
     CDS             complement(2346..3164)
                     /locus_tag="VMZ02_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HUR70708.1"
                     /translation="MEATFIKLTAAIYLFAAASYFYFVFRKDTVSRLPSFALASGFIF
                     HTAALLVRFVNEGFAAVALIGEALLFKSWLMVGVYLLIQVRYRLTVLGGIVAPLAFLM
                     CLAAMAFGKSGAEIPPGLESFWLPLHVTLAFLGNAVFALAFGVSFIYLLEERHLKHKK
                     MTALLKRFPPLESLDRLNLMLLIWGFPLMTLGIITGSLWAGIHWGDYWSWEPRQIASG
                     IAWLFYGAILHGRITAGLRGKKAALLTMVGFCVVLGYFLWGDTVFPSRHGGRFQ"
     gene            complement(3272..4513)
                     /locus_tag="VMZ02_01900"
     CDS             complement(3272..4513)
                     /locus_tag="VMZ02_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HUR70709.1"
                     /translation="MSQSFFFLFFLLLALAGLPVAISAQQAPFPVPSGLKDAVDFWKQ
                     VFTRYGFGDVILFDPLDPVTIYRVLRVSESEQGRALVSKERARVATEYDLVDDDMRLR
                     SQRGAKEQFREGLRISGRYISQMQKIFRDEGLPVQLAYLPLVESSFNVRARSGAGAVG
                     MWQFIPETGKKFMRIDDKVDERRDPMASTRAAARLLKENYRILGSWPLAITAYNHGTE
                     GMFRGIESVGSRDLVELIRRYQSPTFGFASKNFYAEFLAVLEIVGEAEKHFPFLRLHR
                     PVVLQEVEVKKATALQAVLKPAAISQVDFFQWNPALDDGTDVIPAGYRVNLPPERIQT
                     FVRAQARVTPAKSPGGVERKPAGKLVTAQGVGKSGPNASSTTGSRAAVSIAAKGPNTT
                     RTKEQVVKELAARHQLNIAAQ"
     gene            4583..6109
                     /locus_tag="VMZ02_01905"
     CDS             4583..6109
                     /locus_tag="VMZ02_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70710.1"
                     /translation="MPAYFHRSILVFLFVIISSGCRSSHRDVPTNFLVVGIEANPAQL
                     DPRYATDANAVRIGNLIYNSLLRTDEKLRLQPELAQSWHQIDERTFEFGLRPNIKFQD
                     GRPLRAADVKYTFESILDPANRSPKRALLKQLQSIEVDGSHRLRFHLNAPHAPFVEHF
                     TLGIVPQGSSPGTRPPPGSGPFMLEKVESGERVTLTANANSWEGKPALAGLVFKIVPD
                     AMVRALEFKQGAIDLMQNDLEPDMLPWLKNLSDALVSAHQGTTFQYIGINLTHPILQH
                     LKVRQALAHAIDRERIIRHLLKDAAVAANGLISPLNEAYDESIPAWSYDPARAKHLLD
                     EAGFKDPDGDGPLTRFRLSYKTTNIDLRRRIAETLKEQLLQVGIELEIRSYEWGTFFS
                     DVKHGNFHLYSLAWVGIRDPDIQHQIFHSASVPPDGDNRGRYSNRQVDRLLDQARVTM
                     DPSERKRLYGAAQRLIAEDLPYVPLWWQKNVIAKKTSIHGFVPYPDGDLVSLKHVSLR
                     "
BASE COUNT         1429 a         1902 c         1631 g         1301 t
ORIGIN      
        1 tcgggaactc gatcacttcg ccgcccaagg cctcgatacg ttgggccaag ccgcctgctt
       61 gtttacgtgc ccgcgtaata acgatgcgtc gacccttaaa tggtttctcg gaagaaattg
      121 ccccagctat attcggcgtc atgaatcaac tcgcgccaag attttgtccg caccctgtcg
      181 acgcagctca tcggccaaac atgcgccgag ttgctcgcct tcctgggcct cgcccgtgag
      241 ctgcgctctg cacagagtgg cgccatcggg actggcaacc acagcaatca tgttcaaagc
      301 attgccatcg acacgggcgc gggcaccgat gggcacatgg catccgccgc tcaaactacg
      361 cagcaacgca cgctccgcac gcgtttcggc ttcgctcgtc gaattatgta gaaagctcaa
      421 tagctggcgc acggtagcgt cgccacgggt ttcgatggca agcgcgccct gggccacggc
      481 gctgacacaa atctcatccg gcagtatttc gccgatgcgt tcggcgcgac cgatgcggat
      541 caaacctgca gctgccatga tcaaagcatc ggcctcgccc tcatcgagct tgcgcaggcg
      601 agtatcgaca ttgccgcgga tcggcacaac gactaaatcg ccgcggtgat gcagcagttg
      661 agcttgacgg cgaaggctgc cggtggcaat gcgcgcactc ggcgctagat ccgcaatctt
      721 caaaccgttc ctgctgacca ggacatcccg ggcatcctcc cgcactggca ccgcggcgat
      781 ggtcaaccct gcggggagct ccgtcggcaa atccttcatc gagtgcaccg ctagatcgat
      841 tgtttgattg agcagcgcgt cctcgatctc tttggtgaat acacctttgc cgcccaacat
      901 cgatatcggc cggtcgctga aacgatcgcc gctggtcttg atgatcgaca gttcaacctg
      961 aagatcagga tagtgcgcct cgatctgctg cttcacccaa ttcgattggg cgagagccaa
     1021 cgcgctgccc cggctgccga tgcgcacccg gttcatttac cctccaaatc gaagagcttc
     1081 ttaagggcgg ccaggtatag cgcggcttca tcgtcgttat cgcggtcgcc gcggcgcttg
     1141 agctcggtaa tgggcgcgtg cagaaatttg ttgatcatgg ccgtcgtcat gtcctcgagc
     1201 gcggcccgct gttccggcga aagagttttt aggacaccgc caagagattt atcgatttcg
     1261 ccacggcgaa tatcgtcgaa acgttgacgc agagccgtga tcgtcggcac ctgttccaaa
     1321 gagccgatcc agcgcacgaa gctctgcacc tcctcgctga cgattccttc cgctttggcc
     1381 gcttcgttgg aacgcccctg gagattttcc tcggcgacgt ttctaagatc atcgatattg
     1441 taaagataga cattatcgag atcgttgatt ttgccgtcga agttgcgccg atccccgatg
     1501 tcgatgaaaa acatcgcctt gtgcttgcgt tctcgtaaaa ccctatcgac ctgggcagcg
     1561 ttaacgagaa cctccggcgc gcccgtgcag ccgatcacca gatcggccat tttgagatat
     1621 tggggaaagt cttcaaagcg aattgggttg ccatgaatgc gctcggccaa ttccaccgcc
     1681 cgctcaaatg tccggttggt gaccatgaga ctgcgcacgc cgtggctttg cagatggcgc
     1741 gccatcaggt cacccatttt accggcaccg atcagcatta cagtcttgtc gtcgaaacga
     1801 tcgaaaatcc gctttgccaa atcgaccgcc accgaactca acgatacggc gccactgcca
     1861 ataccggttt cgctgcgcac ccgcttggcc accgagaatg aacgatgaaa taaccgatgc
     1921 aggatcgttc ccaccgagcc ggcttgctgc gcggcatcgt aatgctgctt caattggccc
     1981 aatatctgcg gttcaccgac caccatggaa tcgagactgg cggcgacgcg aaatagatga
     2041 cgtatcgctt cgacgccgcg atactggtaa gtatgctccg tcaacgcgat gccatgccgc
     2101 tgtgcctttt gctcactcag aaatcggcca atctcgctat aagcggtcgt actatccgac
     2161 gccgcggcga cgacttcgac gcggttacaa gtcgacagaa tcacaccttc atcgatggac
     2221 gaataattgc gcaaacgagt taaagcgtcg tgcagatagg cgctttcgaa cgcaacgctt
     2281 tcgcgcacct ctatcggcgc gctgcgatgg ttcaatccga caatgacaat ttcttttcgc
     2341 acggcttatt ggaaccggcc tccatgccgg ctggggaaaa ccgtatcgcc ccagagaaaa
     2401 taaccgagca ccacacagaa accgaccatc gtcagcagcg cagccttctt gccgcgtaac
     2461 cccgcagtga tccgcccgtg caaaatcgcc ccataaaaaa gccaggcaat ccccgacgct
     2521 atttgccgcg gttcccacga ccaatagtca ccccaatgaa tacctgccca caggctgccg
     2581 gtgataatgc cgagggtcat caagggaaag ccccagatca aaagcataag gttcaaccgg
     2641 tcgagagatt ccagcggcgg aaagcgtttc aataacgcgg tcattttttt gtgcttgaga
     2701 tgccgctctt ccagcaggta aataaagcta acgccgaaag ccaaagcgaa cacagcgttg
     2761 ccgagaaacg ccagggtgac gtgcagcggc aaccagaacg actccagacc gggtggaatt
     2821 tccgcgcctg atttgccgaa agccattgcc gccaaacaca tgagaaatgc cagcggtgcg
     2881 acgatgcccc ccaatactgt gagccggtac ctaacctgga tcaacagata gacacctacc
     2941 attaaccagc tcttaaatag cagcgcttcg ccgatcagcg ccactgcggc gaacccttcg
     3001 ttgacaaagc gcaccagcaa ggccgccgta tgaaagataa aaccgctcgc caatgcaaac
     3061 gacggcaacc tggagaccgt gtccttgcga aaaacaaaat aaaaatagct cgccgcggcg
     3121 aagagataaa tcgccgcggt taacttgatg aaggtcgctt ccatagacag cggtcattat
     3181 aagctaaccc gatgggcctt caagccgact gacgcagagc taaaccgacc gagagaagcg
     3241 tagctattga gaatttgaca gcgaggcggg gttattgcgc ggcaatattc aactgatgac
     3301 gcgcagctag ttctttaacg acttgctcct tggtccttgt agtgttcgga cctttggcag
     3361 ctatcgaaac ggcagcgcga gagccggtgg tcgagcttgc gttcggccca gactttccca
     3421 cgccttgggc ggttacaagc tttccggctg gcttccgctc gacaccgcca ggggacttgg
     3481 cgggtgttac gcgcgcctgc gcgcgaacaa aggtctgaat ccgttccggt ggcagattca
     3541 cccggtagcc tgccggaatt acatcagtgc cgtcgtccaa ggccgggttc cattgaaaga
     3601 agtcgacttg ggagatcgct gccggcttga gcaccgcttg cagcgcggta gcctttttaa
     3661 cctccacctc ctgcaatacc accggtcggt gcagccgcaa aaacgggaaa tgcttctccg
     3721 cttcgccgac gatctccaat acggcgagaa attcggcgta gaaattcttc gatgcaaagc
     3781 cgaacgtggg agattgatat cggcgaatca gctcgaccaa atcgcgcgaa ccaacgcttt
     3841 cgatgcctcg aaacatgcct tcagtgccgt ggttgtacgc ggtgatcgcc agcggccagc
     3901 tgccaagaat gcggtagttc tctttcaata gacgcgccgc tgcgcgcgtc gacgccattg
     3961 gatcgcgacg ctcatctact ttgtcgtcta tgcgcataaa tttcttgccg gtctcgggaa
     4021 taaattgcca catgccgacg gcaccggcac ccgaacgggc acggacgttg aacgacgact
     4081 ccacaagcgg cagataagcc agctgcaccg gcaatccttc atcgcgaaaa attttctgca
     4141 tttgcgagat atagcgtccg gagattctca gcccctcgcg aaactgttcc ttggctcctc
     4201 gttggcttct taatcgcatg tcgtcgtcga ccaaatcgta ctccgtggcg acgcgtgcac
     4261 gctctttgct aaccaaggcg cggccctgct cggactccga cacccgcaag acgcggtaaa
     4321 tcgtgaccgg gtcgagcgga tcgaacagga tcacatcgcc aaaaccatag cgcgtgaaaa
     4381 cttgtttcca gaaatccacg gcatccttca acccgctggg caccggaaac ggagcttgtt
     4441 gagcggaaat cgcgactggc aagccagcga gcgctagcaa taggaaaaat agaaagaaaa
     4501 aggactgcga catcggaagt ccgaaatttg accgatatta ccaaacaggt tagagtcagt
     4561 caaaatttcc gcagagaaat tcttgccggc ttactttcat cgatcgatct tggtttttct
     4621 cttcgttatc attagctcag gctgccgttc atcacatcgc gatgttccga ccaattttct
     4681 ggtcgtcggc atcgaggcca atccggctca gctcgatccg cgttacgcca ccgacgccaa
     4741 tgccgtgcgc atcggcaatt tgatttacaa ctccctgctg cgcaccgatg aaaagctgcg
     4801 cttgcaaccc gaactcgccc agagctggca ccaaatagat gaacgcacgt ttgaattcgg
     4861 cctgcgaccc aatatcaaat tccaagacgg ccggccgcta agagccgcgg acgttaaata
     4921 caccttcgag tcgatcctcg atccagccaa tcgctcgcct aaacgcgcgc tgctcaaaca
     4981 gctgcaaagt attgaagtcg acggctctca ccggctgcgc ttccacctga acgcgcccca
     5041 tgcgccgttc gtcgagcatt tcacccttgg cattgtgcca cagggatcct cacccggcac
     5101 tcgcccgccg cccggatccg gtccgttcat gctggagaag gtcgagtcag gcgaacgggt
     5161 cacgctcaca gccaacgcca actcctggga aggcaaaccg gcactcgccg gtctcgtgtt
     5221 caagatcgtc cctgacgcca tggtacgcgc cttggaattc aaacaaggcg ccatcgatct
     5281 catgcaaaac gatctcgagc cggacatgtt gccgtggttg aaaaatctga gcgatgcgct
     5341 agtcagcgcc caccagggca cgacatttca atatatcggc atcaatctga cccacccgat
     5401 tttgcaacat ctcaaggttc gccaagcgct cgcgcacgcc atcgaccgtg agcgcatcat
     5461 tcgtcattta ctcaaagacg cggcagtcgc cgccaacggc ttaatttcgc cgctaaacga
     5521 agcctacgac gagagtattc cggcttggag ctatgatccg gcacgcgcca aacacttgct
     5581 cgatgaagcc ggcttcaagg atccggacgg tgacggccca ctgacgcgct tccggctctc
     5641 gtacaaaaca accaatatcg acttgcgccg gcgcatcgcc gaaacgctca aggagcaact
     5701 gctacaagtc gggatcgagt tggagattcg tagttatgag tggggcactt tttttagcga
     5761 tgtgaaacac ggcaactttc atctctactc gcttgcctgg gttggcatcc gcgatccgga
     5821 tattcagcat caaatcttcc attcggccag tgtaccgccc gacggcgaca accgcggccg
     5881 ctactctaat cgtcaagtcg atcgcctgct cgatcaggct cgagttacga tggacccaag
     5941 cgaacgcaaa cgcctctatg gcgcggcgca gcggctgatc gccgaggatc tcccctacgt
     6001 gccgctgtgg tggcagaaaa atgtcatcgc gaagaaaacc agtatccacg gttttgtgcc
     6061 gtatccggac ggcgatcttg tttcattgaa acacgtatcc ctgcgctaaa ctgaagccgt
     6121 gaaaacattc ttctggcgcc gtctgctgct attggtcccg acccttttcg gcgcgctctc
     6181 tttggttttc ctgctcgtgc acttgattcc cggcgatccg gtcgaagtga tgcttggcga
     6241 gaccgctagt ggcgccgata aac
//
LOCUS       DATLCH010000066         7902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10545629, whole genome shotgun sequence.
ACCESSION   DATLCH010000066 DATLCH010000000
VERSION     DATLCH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10545629"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1148)
                     /locus_tag="VMZ02_01910"
     CDS             complement(135..1148)
                     /locus_tag="VMZ02_01910"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUR70711.1"
                     /translation="MTRNIGSEALPAGKEAHLFFGKSPLRVRGSATQGTLKAVEIDQS
                     DSATLTLEKGIRGIFVQRREKPYDVELKTVSAAIENSLFDDGRKAGVHPALLSQLADL
                     FTWDVDLEKDIRKGDNFKILYEQRSRKGQETKTGLRILAAELNSAGQKYTAVYFERQN
                     GVGGYYNQEGRSLARSFLRFPLEFSAITSHFTESRFHPILRTNMPHTGVDFAAQRGTP
                     VRSVGDGIIAEAGWNGGYGKAIDIKHDSTYSSRYAHLDSFAEGIQPGISITKGQVIGF
                     VGSTGRSTGPHLHFELYKDQQYINPLSIDFPAEDHLEPALHRLFENQMRTYMTELKST
                     PQS"
     gene            complement(1650..4226)
                     /locus_tag="VMZ02_01915"
     CDS             complement(1650..4226)
                     /locus_tag="VMZ02_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR70712.1"
                     /translation="MAQPGARGTTIMMNWFNNLEFARPDFLWLLLLLPVLWLRFGDRR
                     FSVLMGRTAILALVILTLAEPQTTSQQSRHEERVFAYDVSRSVPGSIRPWMERTSEEL
                     APGPSDRIYSFAAVTKENASFKEALTNNKGANEPDKTNLENLLTSLLALPAAPRSLFL
                     FTDGWETDGDVERLLPAAAAAGIKIYPMLPAGRPAIANVAVTKVSAPSQGMSGELLNL
                     KVSLDNQNDRAVEGILTLTRNGQSFKTDRVKLRPGSQSFSYQVNLSGEALTAYQASFA
                     ASDGATDAFTADNHALAWVNVRTKAKVLILNGSGGSGRYLDEIVRRQGFDVVSRTADS
                     APAPAGHKVVIFNNVPRERFSANYLASVERHVADGGSFVMLGGDASFSPTSYRQTPIE
                     RILPVEPKEPPKQPEKNRAVILVIDKSGSMRDENRILYAKEAAKAVARQLKDIDLLGV
                     VGFDDSPFVVVYLESVARLRGVIDNQIDRLRPGGQTYFLPALQEARRQLERSNASRKH
                     VILLSDGVTRGSQGELVDLIGSMKNESKITVSAVAISNEADVRVMKRVSQYGGGLFHH
                     TVDPSTLPQIVLEQLQDKPREDPPDNRPLIPVSSGGSELLAGFGVRNYPPVLGYMDTE
                     VKRGAHADVIIPRDDRRAPLLASWRYAKGKSVAFTSDLEGRWTRNWVPWGELQAFWGR
                     ILEWLSPTEQNLIPLHEARVTFSGGQSILDLSVYEDSSANSQFRFSLGSKAGKTDGTL
                     SKLAPGHFQAVLPATSSGDYRVDLIEERVGQRIPFPPIAYTLAYDRRAEVPRPEFNYR
                     LLARLAEASGGEINPRSPNSLRRESVSKSYQPVRQPLLIAALLLFLLEIALRRFVFAE
                     SD"
     gene            complement(4190..6007)
                     /locus_tag="VMZ02_01920"
     CDS             complement(4190..6007)
                     /locus_tag="VMZ02_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR70713.1"
                     /translation="MEFLNPAALFGLLALPVLLIPYLIRRKSQRMAFSSLLLFLESSE
                     AASSKFWGRINLPPIFFLQLLLLALLIIALSEPVFSIHPTNIAVILDNSASMQALEDE
                     KSRFTLAKERASALVSELGAGGKVDLFMTTPRLEKLHGAPLSPAEATRLIATQVPYDL
                     AEPPVDYDNMISQLARQQKYERVYLITDHPARGQSAAARVITVGRPHANFALTALEIQ
                     RSSLANARLEALVEVANYSERDEKLKIILKGSGIELANRVIDVGKGKTTTVSFEGFAD
                     HPTYAAEIANRDALPLDNRRFAVAPGSRNLRVLGITPRPRDLESLKAIPGVQIDTVAP
                     SEYDKAERSGYALEIYHFATPAALPANAALFILPPQSSSIVDLGAAINNVTISNWRDP
                     HVLTRYINFNLFRPTFARPLKPQTAGDIILDSAGGPLAFATERQGVRYLTLGFDPLPY
                     LGRDNLPMSVFTLNLLDWFFDSRGANNQATGEAIFLGKVQSGDRVILPGGKSLTITPG
                     AAFFGGAFFQGIYQLNRGGKTELFARNLFDRGESDLQVSAPLPIGGNQNSPSASLLFS
                     FWPYILMACIALLLLEWFLSPHMTARGRWHSPARVAQRS"
     gene            complement(6012..6905)
                     /locus_tag="VMZ02_01925"
     CDS             complement(6012..6905)
                     /locus_tag="VMZ02_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HUR70714.1"
                     /translation="MLPNPFTPRFLSQLEALRLRTRKEFLGSHTGSYSSPRRGTSLEF
                     ADYRRYAPGDDLRYLDWGIYARTDKLYVKLFREEVDLFAYVFIDASASMGYPSRELKY
                     LPATHVGLALSYVILANHDHVKLHLLQERSGTKATPFYRGRRRMSDCVNFAAAATPSG
                     ALQLAPALGDHLKKMRRPGKAILLSDFLMPASSYETGLNLLRAFNLDIAAIQILSRHE
                     IDPRFPNGSLALIDSETEQEIKYKWNADARRDYQAKLAHHNAELRSFCHQGGIHYSLY
                     VTDRDLSDFVFATLPAIGLFK"
     gene            complement(6919..7611)
                     /gene="queC"
                     /locus_tag="VMZ02_01930"
     CDS             complement(6919..7611)
                     /gene="queC"
                     /locus_tag="VMZ02_01930"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HUR70715.1"
                     /translation="MLNKPAVVLLSGGVDSATAAVIAKRAGFAIHALSFRYGQRHACE
                     IHAASRVANFLTASSHLVIDFDLRAIGGSALTDEIEVPKARSTAEIANGIPATYVPAR
                     NTIFLSFGLALAERIGADDIFFGANQLDYSGYPDCREVYIRAFETMANLATKAGVEGK
                     THIRIHTPLLHMTKGAIIAKGIEFGVDYGLTWSCYDPRDDNLACGLCDSCQLRLKGFR
                     DAGAVDPIAYVE"
BASE COUNT         1780 a         2073 c         2270 g         1779 t
ORIGIN      
        1 atagacctgt gacaatgacc agtagtgtca gtttgcgtcg taggtcgctg acagtgagcg
       61 cgataccatt gagaaaaacc gcaaacgtca ggggtgcgaa cggtaagagt ctgaaaaaac
      121 ggacgggtcc ctggttagga ctggggcgta gacttaagct cggtcatata ggtgcgcatc
      181 tggttttcaa acagccgatg caacgccggt tcaaggtgat cttctgccgg gaagtcgatg
      241 ctcaaggggt tgatatattg ttggtccttg tagagttcga agtgcaggtg cggacctgtt
      301 gatcggccgg tggagccgac aaatcctata acttgacctt tcgtaatgct tatacccggc
      361 tggatgcctt cggcaaaact gtccaaatga gcgtaacggc tggaatatgt tgagtcatgc
      421 tttatgtcga tggctttacc gtagccgccg ttccagccgg cttcagcgat gattccatcg
      481 cccacggaac gcaccggtgt tcctcgctgc gcagcaaagt cgacacctgt atggggcatg
      541 ttggtacgca gaatgggatg aaatcttgac tctgtaaaat gagatgtgat ggcgctaaat
      601 tccagcggaa atcgtagaaa cgaacgcgcc aggcttcgac cctcttgatt gtaataaccg
      661 cccaccccgt tttgccgttc aaagtatacg gcggtatatt tttgtccggc gctattaagt
      721 tcggctgcca gtatacgcaa gccggtttta gtttcttgac ctttgcggct acgttgctcg
      781 taaagaattt tgaagttatc gcctttacgg atgtcttttt ctaaatcaac atcccaggtg
      841 aagagatcgg cgagctgcga gagcaaggct gggtggacac cagctttgcg accatcgtcg
      901 aacagtgagt tctcaatggc ggcgctaaca gtcttcaact cgacatcgta cggtttttca
      961 cgtctttgca cgaaaatacc acggatgcct ttttctaacg ttagagtggc agagtcgctc
     1021 tgatcgattt ctacggcttt cagtgtaccc tgagtagccg agccacgcac ccgcagcggg
     1081 gacttcccga agaataggtg cgcctctttt ccagccggca aagcttcgct gccaatgtta
     1141 cgcgtcatcg tgcgcgccca aagcagactc tcagccttag acacattgtg ccgggttaaa
     1201 acgcttgtca gcgtatcgcc gggtcggatg cgataattta ctagttctcg tttgtcggag
     1261 aaatcgacgg gttgcgagtt gggctgccga agcagagcgc gatgagcctg aatttttgtc
     1321 agccgcgctc ccggagcgct agcctgagca gcaacggggg cgggtgccgg cttagttcga
     1381 acagccggct ttgctggtac ccgctgcttg gccgcctcgc tcggcggggg caaagtcaat
     1441 gccgctaaaa caactgtaga gcagagagct ttcgcaaaaa tcgatacgtg tgggtgtcca
     1501 gatcgacatt cgttgtgctc cccagcgggt atgatttggc aatccatagt aaacaaagat
     1561 tacaacttat agtctgtcgg gcgcggcctg tcaaccgtaa aattgctgtc taaaacgcta
     1621 atagctggga caaatcgcgg tcttaggggc taatcggact ctgcaaaaac gaaccgtctt
     1681 agggcaatct caaggagaaa aagaagcaag gccgcgatca acagcggttg gcgcaccggc
     1741 tggtagctct tcgatacact ttcgcgtctc aagctattcg gcgagcgtgg attgatttca
     1801 ccgccgctgg cttccgccaa gcgcgctagc aggcgatagt tgaactctgg gcgcggcacc
     1861 tcggcgcggc gatcgtaagc caacgtgtag gcgatcggcg ggaatgggat gcgttggcct
     1921 acgcgttctt caatgaggtc gactcgataa tcgccgctcg aagtggccgg tagaacagct
     1981 tggaagtggc ccggcgctag cttgctaagc gttccatcgg ttttacccgc cttgctgcct
     2041 aagctaaagc ggaactgact attcgcactg ctatcttcgt agacagacaa atcgagaatc
     2101 gattggccgc cgctgaaggt gacgcgagct tcgtgtaacg ggattaagtt ttgctcagtt
     2161 gggctgagcc attccaaaat ccggccccaa aaagcttgca gttcacccca gggaacccaa
     2221 ttgcgcgtcc agcgtccttc caagtcgctg gtgaaagcca ctgatttacc tttggcgtag
     2281 cgccaggagg cgagcaatgg cgcgcggcga tcgtctcgcg gaattattac atcggcgtga
     2341 gcgcccctct taacttcagt gtccatataa ccgagcactg ggggatagtt tcttacgccg
     2401 aaaccggcca atagttccga accaccggac gataccggaa tgagtgggcg attgtccggg
     2461 ggatcttcgc gcggtttgtc ttgaagctgt tcgaggacga tttgcggtaa ggtcgacgga
     2521 tcaacggtgt ggtggaacag accaccgccg tattggctca cgcgcttcat gactctcacg
     2581 tcggcttcat tactgatcgc tactgcggat accgtaattt tgctttcatt cttcatgctc
     2641 ccgatcaggt caacaagctc gccttggctg ccgcgagtta cgccatcgct gagcaagatc
     2701 acgtgcttac gcgacgcgtt tgagcgttcg agctggcgtc tggcctcttg cagagcgggc
     2761 aggaagtagg tctgaccacc gggcctgagg cggtcgatct gattgtcgat gacgccgcgt
     2821 agtcgcgcca ctgattccaa atagacgacg acaaagggtg aatcgtcgaa gccaacgacg
     2881 cccaacaaat cgatgtcctt gagctggcgt gccacggcct tggcggcttc cttggcgtac
     2941 agaatacgat tttcgtcgcg catgctgccg gacttgtcaa tgaccaagat caccgcgcga
     3001 tttttttccg gctgtttggg cggttctttc ggctccaccg gcaagattcg ttcaatcggg
     3061 gtttggcgat agctggtcgg agaaaagctt gcatcgccac caagcatgac gaatgaaccg
     3121 ccgtcggcga catggcgctc gacgctggcg agatagttgg ctgaaaaacg ttctcggggc
     3181 acattattga agattacaac tttgtgcccc gcgggtgcgg gtgcgctgtc ggcagtccga
     3241 gaaaccacgt cgaaaccttg acgacgaacg atctcgtcga gatatctgcc ggaaccgccg
     3301 gagccattta agatcagtac ttttgccttg gtccgcacat tgacccaggc cagcgcgtgg
     3361 ttatccgcgg tgaatgcatc ggttgcgccg tcgctggcgg cgaagcttgc ttggtaggcg
     3421 gtgagcgcct cgccgctgag attgacttga taactgaaac tctggcttcc tggacgtaac
     3481 ttgacgcggt cagttttgaa cgattgacca ttgcgagtga gggtcagtat gccctcgacg
     3541 gcccggtcat tctgattgtc caagctgact ttcaggttga gcaattcgcc actcataccc
     3601 tggctcggcg cgcttacttt cgtcactgcc acattggcaa tcgccggtcg gccagccggc
     3661 agcatgggat aaatttttat cccagccgcg gcagcggcgg gcaggagccg ctcaacatcg
     3721 ccatcggttt cccaaccatc ggtaaacaaa aaaagcgaac gcggcgccgc cggcagagcc
     3781 agcagtgaag tcaaaagatt ttccaaattg gttttatccg gttcgttggc gcccttgttg
     3841 ttggtgagcg cttctttgaa actggcattt tcttttgtca cggcggcgaa actgtaaatg
     3901 cggtcgctcg gaccgggggc taattcctcg ctggttcgct ccatccaagg acgaatgctc
     3961 cccggcacgc tgcgcgagac atcataggca aacacacgtt cttcatggcg cgattgttga
     4021 ctggtagttt gcggttcggc aagcgtgaga atcaccagtg ccaggatcgc cgtgcgcccc
     4081 atcaggaccg agaaacggcg gtctccgaag cgcagccaaa ggactggtaa caggagcaga
     4141 agccacaaga aatcgggccg ggcaaattca agattgttaa accagttcat catgatcgtt
     4201 gtgccacgcg cgccgggctg tgccatcgtc ctcgcgccgt catgtgcgga cttagaaacc
     4261 attcgagcaa taacagagct atgcaagcca tcaatatata aggccagaac gaaaacagca
     4321 gcgaggcact ggggctattc tgattaccgc cgatgggaag gggtgctgat acttgcaggt
     4381 cggattcgcc gcgatcaaac aaattgcggg cgaacaattc ggtcttaccg ccgcgattta
     4441 gctgataaat accctgaaaa aatgctccgc caaagaacgc tgcacccgga gttatggtta
     4501 ggcttttgcc gcccggcaag ataactcggt cgccgctttg cactttaccg aggaagattg
     4561 cttcaccagt ggcttggttg tttgcgccgc ggctgtcgaa aaaccagtcg agtaagttta
     4621 acgtaaacac ggacatcggc aagttgtcgc gccccaaata cggcaaagga tcgaaaccaa
     4681 gagtgagata tcgcacccct tgtcgttccg tcgcgaacgc caaaggaccg ccggcgctat
     4741 cgagaatgat atctccggca gtttgcggct tcagcggccg ggcgaatgtc ggccggaaca
     4801 agttaaagtt gatgtagcgg gtgagcacat gtgggtcgcg ccagttggag atcgtcacgt
     4861 tattgatagc cgcgccgaga tccacgatag aactgctttg cggtggcaga ataaatagcg
     4921 ccgcgttggc tggcagtgcc gctggagtgg cgaaatgata aatttccagc gcgtaaccgc
     4981 tgcgctcggc tttgtcatac tcactcggcg cgacggtatc gatctgcacg ccgggaatcg
     5041 ccttcaaact ttccaagtcg cggggtcttg gcgtaattcc caatacgcgt aaattgcgcg
     5101 agccgggcgc cacggcgaag cggcgattat ccagcggaag agcatctcgg ttggcgatct
     5161 cggccgcata ggtcggatga tcggcgaaac cctcgaatga aaccgtcgtc gtcttgcctt
     5221 tgccgacgtc gattacccgg ttcgccagtt cgatgccact gccttttaga ataattttca
     5281 atttctcatc gcgctcggaa taattggcga cctcgaccag agcttccaaa cgcgcgttgg
     5341 cgagcgagga acgctgtatt tccaatgctg tcagcgcaaa attagcgtgc ggccgtccca
     5401 ctgtgatcac gcgcgcggcg gcgctttggc cgcgcgccgg gtgatcggta atcaaataaa
     5461 cgcgttcata tttctgctgg cgcgccaact gagagatcat gttgtcgtaa tcgaccggcg
     5521 gttcggccaa gtcatagggt acttgcgtag cgataagtct cgtcgcttcg gcgggcgaga
     5581 gcggcgcacc gtggagtttt tctaaacggg gcgtagtcat gaagagatcg accttgccgc
     5641 cggcaccgag ttcgctgacc agtgcgcttg cacgttcctt cgcgagcgta aagcggctct
     5701 tttcgtcttc cagtgcctgc atgctggcgg aattatcaag aatcacggct atattggtcg
     5761 ggtgaattga aaataccggt tcactcaatg cgatgatcag caaagccaac agtaataatt
     5821 gtaaaaaaaa gatcggcggt aggttaatgc gtccccaaaa ctttgaactc gccgcttcgc
     5881 tgctttcaag aaagagcaac aagctcgaga acgccatccg ttgggattta cgccggatca
     5941 aatatggaat cagcagcacc ggcagtgcga gaagaccgaa cagggcggca gggttgagaa
     6001 actccatcgg attacttgaa caaaccgatg gccggcaagg tggcgaagac gaaatcgctt
     6061 agatcgcgat cggtgacata gagagagtaa tgaataccgc cttggtggca gaaactgcgc
     6121 agttccgcat tgtgatgcgc taattttgcc tgataatcgc ggcgggcgtc ggcgttccat
     6181 ttatacttga tctcttgttc ggtttcgctg tcgatcaggg ccaagctgcc attgggaaag
     6241 cgtggatcga tttcatgacg ggagaggatt tgaatggcgg caatgtcgag attaaacgcg
     6301 cgcagcaaat tcaggcccgt ctcataggat gaggcaggca tcagaaaatc agagaggaga
     6361 atcgctttgc ccggccggcg cattttcttc aaatggtctc ccagtgcggg agcgagctgc
     6421 agcgcgccgc tgggagtcgc ggcagcagcg aagtttacgc agtcgctcat gcgccgccgg
     6481 ccccgataga agggcgtggc tttagtaccg cttcgctcct ggagcaagtg caattttaca
     6541 tgatcatggt tggccaagat cacgtagctg agcgccaagc cgacgtgagt cgccggcaaa
     6601 tatttcaatt cgcgcgaggg atatcccatc gaggcgctgg catcgataaa aacatacgcg
     6661 aatagatcta cttcctcgcg aaacagttta acgtagagct tatccgtccg cgcgtaaatc
     6721 ccccaatcga gataacgcaa gtcgtcaccc ggagcgtaac ggcgataatc ggcaaactcc
     6781 aaactggtgc cgcgccgggg cgacgagtag ctgcctgtgt ggctgccgag aaattctttg
     6841 cgcgtacgta gacgcaaggc ttcgagctgg ctgagaaatc ggggtgtgaa ggggttgggt
     6901 aacatgtgtc gtaacgcctt actcaacgta agcgatggga tcgaccgcgc cggcatcacg
     6961 aaaccctttg aggcgcagct ggcagctatc gcacagtccg caggcgagat tgtcgtcgcg
     7021 aggatcgtaa caactccagg tgaggccgta gtcgacgcca aattcgatgc ctttcgcgat
     7081 gatggcgccc tttgtcatgt gcaaaagcgg cgtatgaatc cttatatgag tcttaccttc
     7141 gactccggcc ttggtggcaa ggttggccat cgtctcaaaa gcgcgaatat acacttcgcg
     7201 gcagtcaggg taaccgctgt aatcgagctg attggcaccg aagaaaatat cgtcggcgcc
     7261 gatgcgttcg gccaaggcga gtccgaatga caagaaaatg gtgttgcgtg cgggaacgta
     7321 cgttgccggg ataccattgg ctatttccgc cgtgctgcgg gccttgggaa cctcgatttc
     7381 atcggtaagc gccgaaccgc cgatggcgcg cagatcgaaa tcaatcacca aatggctgct
     7441 tgcggtcaag aaattggcca cacggcttgc cgcgtggatt tcgcacgcgt gacgctggcc
     7501 ataacggaag ctgagcgcat gaatcgcgaa accggcgcgt ttggcgatga ctgcggcggt
     7561 cgctgaatcc acgccgccgc tgagcaagac aacggcaggt ttgttcaaca taatttagga
     7621 acgcggaacc ttagttcctt ccatcggata atgtttctga taccacccaa cgattccttc
     7681 attgagaacc ttcatattgc gataaccgtt ttcgtggaga atgccataag ccaaactggc
     7741 caggtgatgg gggcacgctc aatagagtac cacgagccgg tcgtgcggga tgcttttcaa
     7801 atattctttg aactccgcca gcggcgcgga ctgggcgccc aggatgtgta cctcgtcgaa
     7861 ctcttctttc gttcgcacat cgatcagcat gggcttggca cc
//
LOCUS       DATLCH010000067         3839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1713845, whole genome shotgun sequence.
ACCESSION   DATLCH010000067 DATLCH010000000
VERSION     DATLCH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713845"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1532
                     /locus_tag="VMZ02_01935"
     CDS             480..1532
                     /locus_tag="VMZ02_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70716.1"
                     /translation="MNRQKNLSSSIGFATFISTIVVALVICGASAGKLFAAAADSLDQ
                     LYEKAKKEGKVTLYAPLSPNAMSVIPPAFMKRFPAVTVDHIDATSDKLIARVLAEQKG
                     GRVIADVISGALTYLPQILDLKIVEHVAIPEAAAYPAQLKGDLWVATDTQYYIAGWNT
                     SLIKKGEEPKSYEDLANPKWKDNIMGEPRDYQMLVGFAKRKYNSDEKAIELFRKIAAN
                     QVEFHRGHSQLTEFLMAGQRAICFTCYSHHFPLRMKKGAPIQPLMTEGVGEVGGSVMV
                     MKGAPHPNAALLWVRWAVSEEGQKVYAQAGETPAHPKVEPLEKIRPATPYMLTIDDVR
                     EFPKYEKTWKEIFQIR"
     gene            2234..>3839
                     /locus_tag="VMZ02_01940"
     CDS             2234..>3839
                     /locus_tag="VMZ02_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HUR70717.1"
                     /translation="MADKIVRLDSRRDVEAIAWAKELRETDAWFHSARFRHITRLHTS
                     AEVVSLRGNLQADHTIAKQSALKLFDYLQRLFRDKKQEITYGPYSPTGAVRAVMEGIK
                     VLYLGGWATSAKGSESEDPGADLANYALDRVPKEGGAWVRALMHQDEVQRSRRIRMTS
                     LQRKKTRPIEFSPPLIIPDGDTGHGGEHHTRNLVKTFVENNIGSIHIEDQRGGSKVCG
                     HQGQKVLVSTSEMIARLNAARLQYDILHVPGVIVARTDSNDATAIDSVEDERDHPFVY
                     GATNPHIVPFKNLSLAVIRKFYQKGFKEINGHLLYKISEQAYADAEKWLKQERLFDRI
                     SEGIERIEHEIAALKQLRRQVRRPGKGQRDFREQLAALEIGVKKVMEETVDEVIASIR
                     QAWAEKARLKTFAEAVTEVLTSGKGQQAISVERWQKFAASVSYAEARARAKSLGIEVF
                     WDWDLPRTPEGFYVITGGRAMATARGLATAPFADLIWRETAKPDLADDKAWADEIHAV
                     HPEKMLAYNLSPSWNWDVWGFTDDQIR"
BASE COUNT          913 a         1042 c         1076 g          808 t
ORIGIN      
        1 gaattcattc tcccgtatga tgaagtccgc agagcaaact caccggaaga tacgcttctc
       61 gattttctgc agacaactta cgaagcagca gccgagttgg cgcactggga tcgcgcatcg
      121 ctcgaagggc tgcgctacct ctagtgtgtt tccatcacgc cggtatctat cgtcgcgaca
      181 tcaacgctga gcacatcggc ggccgttctg ttgctcacca gcgaagccgg ccatccgatt
      241 atcacgttcg ctgaaatcgt cggccgcaaa caagaaatta gggccagcat ccgacccgat
      301 gcaggcggga gtccagcctg acacaagcag cgatgctcct aggtagccgg cttaaggttt
      361 gacaacaccg gggttttcct acatggagga gttgcagaga ccctcacgcg ggcgtccaca
      421 accaaggaga gttttcggat attgcttcag aatattgtaa cgaagatacg gagaaacaaa
      481 tgaatcgaca gaaaaattta tcgagctcga tcggattcgc aacctttata tcgacgattg
      541 tcgtggcgct ggtgatctgc ggtgcttctg ccggtaagct cttcgcggcg gcagcggatt
      601 ccctcgatca actttatgag aaagcaaaaa aggaagggaa agttacgctt tacgcgccgt
      661 tgtcccccaa cgcgatgtcg gtgattccgc ctgcgttcat gaagcgcttt cccgccgtca
      721 ccgtcgatca tatcgatgcc acgagcgata agctgatcgc gcgagtgctc gcggagcaga
      781 aggggggacg cgtcattgcc gacgtcataa gcggagcgct aacctatttg ccgcaaatct
      841 tagacctgaa aatcgtcgag cacgtcgcca tacccgaggc agccgcgtat cccgcccagt
      901 tgaaaggcga cctttgggtc gccaccgata cgcaatatta tattgccgga tggaacacga
      961 gtctgatcaa gaaaggcgaa gagccaaaaa gctacgagga cctcgccaac cctaaatgga
     1021 aagataacat catgggcgag ccgcgcgatt accaaatgct cgtcgggttt gccaaacgaa
     1081 aatacaatag cgatgaaaag gccatcgagc tgttcaggaa aatcgccgcc aatcaagtgg
     1141 aatttcatcg cggccattcg cagttaaccg agttcctcat ggccggccag cgtgccattt
     1201 gttttacttg ctactcgcac cattttcctc tgcgcatgaa aaaaggcgcg ccaatccaac
     1261 cgctcatgac cgaaggtgtg ggcgaagtcg gcggctctgt gatggtcatg aagggggccc
     1321 ctcatcccaa tgccgcacta ctctgggtcc gttgggccgt cagtgaagaa ggacaaaagg
     1381 tttatgctca agcgggcgaa actcccgctc atcccaaagt agaacccttg gaaaaaatcc
     1441 ggcctgcgac gccctacatg ctgaccatcg acgacgtgag agagtttccg aagtacgaga
     1501 aaacgtggaa ggaaatcttt cagatccggt gaggttcggg agcggtctta cagctcacta
     1561 gccgcggcac gatcgccaga attgtcaacg ccgctgttgg cgatcggcag gcaatatcct
     1621 tcgaccgtgg tccggtctgg tttgttggtc gggaaaaact tggcgtattc ctcactggct
     1681 gtaaggtcct cgcgttcgtc agaacagcga ctccgcgcgc ttcccaaagc atcttgatgc
     1741 gttcgatggc gtacctcgtc tttgcgaagt tctctgcgga cctcctccgt aagggggtgc
     1801 cgccggtttg accggccacg aacaccagat tgtcaaactg tgtggctggg gagagattgg
     1861 atagcggcag caagattgac ctgaacaatt tgttgcctcg gcatctgacc tctctcaaca
     1921 gaagttttca aatctggatc tgcggcctgc cataagctgc atacccgccg cgatggatgt
     1981 ttggccgctg tgtatgcgcg tcgaacaatg accggtcgcc gaaaacttta ggataccgcc
     2041 agatctattg ctagctaact aaccgcgtcg caagcgggcc ttgcgtccag atagtattgc
     2101 ttcaatttgc cgtagtgtta gtaatgagcc gggagtgatt tgccgatttg tttttgtaac
     2161 cttcataagc cgtcaggaaa agttttatgg ctaaataacc gatcttagac ctaccctaag
     2221 gggggcttac gccatggctg acaaaatcgt tcggttggat tcacggcgtg acgtcgaggc
     2281 gattgcctgg gcaaaggagc tgcgcgagac cgatgcttgg tttcatagcg cgcgcttccg
     2341 ccatattacc aggctgcata cttccgccga ggtagtttct ctgcgcggta atctgcaggc
     2401 cgatcacacg attgccaagc aatcggcgct aaagctcttc gactatctgc agcgtttatt
     2461 tcgcgacaag aaacaagaaa taacctacgg cccgtattcg cccaccggcg cggtgcgcgc
     2521 cgtcatggaa ggaatcaagg tgctttatct cggaggctgg gcgacctccg ccaagggctc
     2581 cgagtcggag gacccgggcg ccgacttggc caactacgct ttggatcggg taccgaaaga
     2641 aggcggcgct tgggtgcgcg cgctgatgca tcaagacgag gtgcaacgtt cccggcgcat
     2701 tcgtatgaca tcgctccaac gaaagaaaac tcgccccatc gagttcagcc cgccgctgat
     2761 aattccggac ggcgacaccg gccatggcgg cgagcatcac acgcgcaacc tggttaagac
     2821 attcgtcgaa aacaacatcg gctcgatcca tattgaagat caacgaggcg gctcgaaggt
     2881 atgcgggcat cagggacaga aagttttagt gtcgacctcc gagatgattg cgcggctcaa
     2941 tgccgcgcgg ctccaatatg acatcctgca tgtgccgggc gtaatcgtcg cccgcaccga
     3001 ctccaatgac gccactgcca tcgatagcgt cgaggacgaa cgcgaccatc catttgtcta
     3061 cggcgcaacc aatccgcata tcgtgccttt caagaatctc agccttgcgg tgattcgaaa
     3121 gttttatcaa aaaggcttca aggaaattaa cggccatctg ctctacaaaa tttccgagca
     3181 agcttacgcg gacgcggaaa aatggctcaa gcaagagcga ctctttgacc gcatttccga
     3241 aggcatcgag agaattgagc acgaaatcgc cgcgctcaaa caattgcgcc ggcaggtgcg
     3301 ccggccgggc aagggccagc gcgattttcg cgagcagctg gcggcgctgg aaatcggtgt
     3361 gaaaaaggtc atggaggaaa ctgtcgatga ggtgattgcc agtatccgcc aggcgtgggc
     3421 tgaaaaagcc cggcttaaga ctttcgccga agcggtaacg gaagtgctga cgagcggcaa
     3481 aggccagcag gcgatatccg tcgagcgctg gcagaaattt gccgcaagcg tctcttatgc
     3541 tgaagcgcgc gcccgagcta aatccctggg cattgaggtt ttctgggatt gggatctgcc
     3601 gagaacaccg gaaggtttct atgtcatcac gggcggccgc gccatggcga ccgcccgagg
     3661 attggccacg gcgccgtttg ccgatttgat ctggcgcgaa acggcgaagc ccgatctcgc
     3721 agatgacaaa gcttgggccg atgagatcca tgccgtccac ccggaaaaaa tgttggctta
     3781 caacttgtcg ccgtcgtgga attgggatgt ttggggattt accgacgatc agattcgta
//
LOCUS       DATLCH010000068         4058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2462318, whole genome shotgun sequence.
ACCESSION   DATLCH010000068 DATLCH010000000
VERSION     DATLCH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462318"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VMZ02_01945"
     CDS             <1..757
                     /locus_tag="VMZ02_01945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR70718.1"
                     /translation="GAAPLGRALAAQEFQPQVGQDGKDVIWVPTPQELVDKMLEMAKV
                     TPKDYVIDLGSGDGRTVITAAKRGSKALGIEYNPDMVELSKQSAAKEGVSDKASFVKA
                     DLFESDFSQAQVITMFLLSSINLKLRPKILELKPGTRIVSNTFDMSDWKPDETAILPG
                     CNSWCTAHLWIVPAKVAGTWMLPQGELTLKQTFQNITGVLKSAGVETPVNGKVNGETI
                     NFTAGNAEYSGRVAANSIEGTIKNGATWTATRR"
     gene            886..1701
                     /locus_tag="VMZ02_01950"
     CDS             886..1701
                     /locus_tag="VMZ02_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUR70719.1"
                     /translation="MDLRKLIASAFVSATLIVNVAGNLSFAQPAKEFQPEVGQAGKDV
                     VWVPTPQALVDKMLEMAKVTPKDYVIDLGSGDGRTVITAAKKGSKALGIEYNPDMVEI
                     SKKAAAKEGVSDKASFMKADLFESDFSQAQVITMFLLPSINIKLRPKILDLKPGTRIV
                     SNSFDMEDWKPDQTEKVEGCTNWCTAHLWIVPAKVQGTWKTPQGELALKQTFQNITGT
                     LKNGNVVTPITGKLNGEQISFSGAGVQYTGQVQGNTMELNGGSAKLTATRAAK"
     gene            complement(1934..3013)
                     /locus_tag="VMZ02_01955"
     CDS             complement(1934..3013)
                     /locus_tag="VMZ02_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70720.1"
                     /translation="MNRWPYLPYSALRLALASATLALIGLAVEPIHAQAPFFQGKTVT
                     IIQGRDPGGTGDARTKALVPFLQKYLPGNPTIMMEYMPGAGGRMAANHLFRTAKPDGL
                     TIGNPSIGMISAAILGEAGVQYDIDKFSYLGSPYSEYHAVFLSRHEAGFTSVEKLQAA
                     SGVRIGAQSVGFVTYNEGRLFAYLLGLKEPRFVAAYSGAELDPALIRGEIDARATGAD
                     SITQRNKEWLEKGSPVDFHAILEVPKGNKHGQFPQLREIESFAKSDLDRKLANMARGF
                     RIAGTPFVLPPGTPKDRVEIIQAAFRKTFQDPDFSRQYKKLSGDEATPLLPENHEKAI
                     REIPRDREVVELFKKLVGAGPLPPR"
     gene            complement(3191..3583)
                     /locus_tag="VMZ02_01960"
     CDS             complement(3191..3583)
                     /locus_tag="VMZ02_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR70721.1"
                     /translation="MLQTQGMLHFSLAVTDLDRSRKFYEGVLNLKVVEQSPRMVFLRT
                     GDDYLILAKGDQPLKYDSNKSTPVHHAFRVKPEEFHSSIDHLREHGVEVFNIEDRNTG
                     VFWGPQAYFLDPDGNKLEIYAGPGAKHD"
     gene            complement(3844..>4058)
                     /locus_tag="VMZ02_01965"
     CDS             complement(3844..>4058)
                     /locus_tag="VMZ02_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70722.1"
                     /translation="LMWHGHFNTGKGNMRHFAMRGDSPKYSHDRFRNPAWTMIPMDEE
                     DPAIQREYVEILERNGVEAAVQVVED"
BASE COUNT          962 a         1132 c         1060 g          904 t
ORIGIN      
        1 cggcgccgcg ccgctggggc gcgcgctcgc cgcgcaagag tttcagccgc aggtcggcca
       61 ggatggtaaa gatgtgatct gggtgccaac tccgcaggag ttggtcgaca agatgctcga
      121 gatggccaag gtgacaccca aagattatgt catcgatctc ggttcaggcg acgggcgcac
      181 cgtcatcacg gcggcaaaac gagggtccaa ggcacttggc atcgaatata accccgacat
      241 ggtggaactt tccaaacaga gcgcggcaaa ggaaggcgtc agcgacaagg cgagcttcgt
      301 caaggcggat ttattcgaaa gcgatttctc ccaggcgcag gtcattacca tgtttttgct
      361 gtcgagcatc aaccttaaac tgcgcccgaa aattctcgaa ttaaaacccg gtacgcgcat
      421 tgtttcgaac actttcgaca tgagtgattg gaaaccggat gaaactgcga ttttaccggg
      481 ctgcaattcg tggtgcactg ctcatttgtg gatcgtgccg gccaaagtcg cgggcacatg
      541 gatgttgcct cagggtgaac tgacgctcaa acagactttt caaaacatta ccggcgtgct
      601 gaaatccgcc ggggtcgaaa cgcccgtcaa tggcaaagta aacggtgaaa ccattaactt
      661 tacagccggc aatgccgagt atagcggtcg cgtcgccgcc aatagcatcg aaggcacgat
      721 caaaaatggc gccacatgga cggcaactcg gcggtaagca attcgtctaa gaccattgtc
      781 gcagcacagc cggcttctcg ggttgactat tttacgacgg aaaatcggcc ggcctctcca
      841 ctattgtaaa tgcgcctgct atatttgcgc gctaggagga tcaacatgga tctaagaaaa
      901 ttgattgcca gcgcatttgt cagtgcgact ttgattgtca atgtcgcggg gaatttaagt
      961 ttcgcgcagc cagcgaagga atttcaaccg gaagtcggcc aggccggtaa agacgtcgtc
     1021 tgggtgccga cgccgcaggc gctcgtcgat aaaatgttgg agatggctaa ggtcacaccc
     1081 aaagattacg tcatcgatct cggctccggc gacggccgca cggtgatcac ggccgcaaaa
     1141 aaggggtcca aggccctcgg catcgaatac aatcccgaca tggtcgagat ttcaaagaaa
     1201 gccgccgcca aagaaggcgt tagcgacaaa gcgagcttta tgaaggccga cctgttcgag
     1261 agcgacttct ctcaagcgca ggtcatcact atgtttttgc tgccgagcat aaacatcaag
     1321 ctgcgcccga agatcctcga cctgaagccc ggcacgagga tcgtgtcgaa ctcgttcgac
     1381 atggaagatt ggaagccgga tcagactgaa aaagttgagg gatgcacgaa ttggtgcacc
     1441 gctcatctgt ggatcgtccc tgccaaagtc cagggcacct ggaaaactcc ccaaggcgag
     1501 ctcgcgctca aacagacttt tcagaatatc accggcacac tcaagaacgg caacgtcgtc
     1561 acaccgatca ctggcaaact gaacggcgaa caaatcagct tttccggcgc cggcgtgcaa
     1621 tacaccggac aagttcaggg caataccatg gaactcaacg gtggcagtgc caaattaacc
     1681 gccactcgcg ccgctaagta aacaaatcaa cttgagataa aaaagccgcg cactctaaag
     1741 tgcgcggctt ttttatcggc tgcgttcacc gtcacgccat cgagaacgcg acacacgggt
     1801 ctactctttg ttgggtggtg ttagatagct tcatcggccg ttaccaacta cgccatcgtc
     1861 gccgtatcgg agcaatacca gcgacatcgg cctcggcgag tagccttgca gtctgcaaat
     1921 gggcacgggc gaatcaccga ggcggcagcg gtccggcgcc gacaagtttc ttgaatagtt
     1981 cgacgacctc gcgatcgcgc ggaatttccc taatcgcctt ttcatggttt tccggtaaca
     2041 gcggcgttgc ctcatccccg ctcaattttt tatattgccg cgagaaatct ggatcctgaa
     2101 aggtctttcg aaaagcagcc tggataattt caacgcgatc cttcggtgtt cccggcggta
     2161 gcacgaaagg cgtgccagcg attctaaaac cgcgcgccat attggccaac ttgcgatcta
     2221 aatccgattt ggcaaagctc tcgatttccc gtaactgggg aaattgccca tgcttgttgc
     2281 ctttgggaac ttcgaggatc gcgtgaaagt ccacgggtga acctttctcg agccactcct
     2341 tattacgttg ggtaatcgag tcggcgccgg tcgcgcgcgc gtcgatctcg ccgcgaataa
     2401 gcgctggatc gagttcggca ccgctataag cggcaacgaa ccttggctcc tttaaaccca
     2461 atagataggc gaataagcgc ccctcgttgt aagtaacgaa accaacggac tgagcaccga
     2521 tacggacccc tgatgccgct tgcaattttt caacgctggt aaaccccgcc tcgtgtctgc
     2581 tgagaaagac cgcatgatac tcgctgtaag gcgaacccag gtaggaaaac ttatcgatgt
     2641 cgtattgcac tccagcctcg ccaagaattg cggcagaaat cataccgata ctcggattgc
     2701 cgatggtgag cccgtccggt ttcgcggttc ggaacagatg attggccgcc atccgccctc
     2761 ccgccccggg catgtattcc atcatgatgg taggatttcc gggaagatac ttttgcagaa
     2821 acggcactaa agccttggtt ctcgcatctc ccgtcccgcc aggatcgcgt ccctggatta
     2881 tggtaaccgt cttaccttga aagaacggcg cttgcgcatg gatcggttct acagctaagc
     2941 cgattaaagc gagcgtcgca gaggctaacg ccaatcgcag cgcggaatac ggcaagtagg
     3001 gccaacgatt catcgtttct cccgttttta cacggtagat tgggacacta aacctttcga
     3061 tttcaatatc cagcgcttca cgagcagcgc aaaaccgttc ctgttttacg acctatacgg
     3121 gcaagacaat tttccgaaac taccgcagcg tcgtcagaga gctggcgtat caatcttgaa
     3181 cagcaatcgg ttaatcatgc ttcgcccctg gccccgcgta tatttccaac ttgttgccat
     3241 cggggtccag gaagtaggcc tgtgggcccc agaacacgcc ggtgttgcga tcttctatat
     3301 tgaacacttc gacgccatgc tcacgcagat ggtcgatgga ggaatggaac tcttcgggct
     3361 ttaccctgaa cgcgtggtgc accggcgtgc tcttgttgga gtcatatttt agcggttggt
     3421 cacccttcgc cagaatcaga tagtcgtcgc ccgtgcgcaa gaataccatg cgcggcgatt
     3481 gctcgacgac tttcaagtta agcacccctt cgtaaaattt ccggctacga tccaaatcgg
     3541 ttaccgccaa actgaaatgc agcatgcctt gagtctgtaa catttacgtt tcctccctta
     3601 cctttggatg ctgtcgcctt ttgatcaacg ttgcgttacc cgcaccgttt agatttcttc
     3661 gaagagaact caactgtgcg ttcataattc cggcatgtgt cgtcacccgg gtgaaaaccg
     3721 gggtccattc ttaaccctaa gtcctggata ccgttttcca acggcatgag gacactatcc
     3781 gtcacctgga tgccatctgc cagcattgcg gatattccat tcgcgcctaa gctgcgtgaa
     3841 ccatcaatcc tccaccacct gcaccgccgc ctcgacgccg ttgcgctcga ggatctcgac
     3901 gtactcgcgc tggatcgccg gatcttcctc atccattggg atcatcgtcc aggccggatt
     3961 gcgaaatcga tcatggctgt atttgggact gtcgccgcgc atggcgaagt ggcgcatgtt
     4021 gcctttcccg gtattgaagt ggccatgcca catcagat
//
LOCUS       DATLCH010000069         5011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9167430, whole genome shotgun sequence.
ACCESSION   DATLCH010000069 DATLCH010000000
VERSION     DATLCH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9167430"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1227
                     /locus_tag="VMZ02_01970"
     CDS             175..1227
                     /locus_tag="VMZ02_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HUR70723.1"
                     /translation="MDVMAAELKTSARRWFVWAAALVITLGSLAGIRTLLSWCNDSHR
                     VVELLNRLESEVNYLGALKWESTARKGVSEEAHELQFSTQQEISQVLDDLDRLVSNGG
                     ITEIRKLYQDYVRSSEVQFRLIERGRYVEAQRVDQDKIDPMYNALIGLMNLMRERHTQ
                     SAHQKMLLVDFGISAVLLLAAVSVGMLFSRFQQGLRIKHVLLVEQNALRKSESRFRSL
                     VQNSSDVIAILNPLPATFIFLSDSVRRILGYRPDQLIGTDIGKMIHPGDSATLQRFLA
                     NCAYSNGLTHAAELRMRRSDNQWTIVEMFGDNRVNDPDISGIVINFRDISETRQIEAA
                     LTQEGYEFDPAEKKLH"
     gene            1503..3200
                     /locus_tag="VMZ02_01975"
     CDS             1503..3200
                     /locus_tag="VMZ02_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70724.1"
                     /translation="MAYDLVIRGGRVVDGSGLPSYVADVAVKDGKVAEIGKVKDAAAK
                     TIDAGGLAVSPGFIDHHTHLDAQMLWDPYGTCEPQHGVTSVVMGNCGLSLAPVRQGIE
                     DAIVKSFVRVEAIPRIALEQGVKWNWHSYGEYLDNLEGKLGINVGGLVGHIAVRHHAM
                     GEEAVERKATGTEVQKMKGLVLDAMEGGALGMSTNRNDRHMREDGKPVSSRLADDDEL
                     FALCDVLGERNAGVIETILGRNKVEHFAWYHELAKRTQRPILWQSLQHRWSEPNLWRE
                     QLEAVEPIFKAGYQAYGLTHTVPLVRHFTLKDCQIFDEFPTWKNLMFLPVEVRKQAFA
                     DADTRTKLQHEIDNLRLTNYHKRWDIPKVEKCFKPENQKYVGKTIAEMAAMRNQAPID
                     AFLDLALDENLETIFWNANNGGDWNAMGEILRSPYVLIGTSDAGAHVLFGADFGYGTI
                     LLGLWVRERQVMSLEQAIHKLTFHPASIFGLQGRGLLRPGYAADIAIFDPKTVNAEEP
                     VWADDFPANSKRMVQQATGMHYTIVNGTVINDHGHMTGDLPGQVMRGPLYRQQKAAA"
     gene            3202..3525
                     /locus_tag="VMZ02_01980"
     CDS             3202..3525
                     /locus_tag="VMZ02_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR70725.1"
                     /translation="MGVSYIPVARIGELSAGAMKEVDLGGRQVLVAFVEGKYYAFSRH
                     CPHEEADLKEAGQLLGNTQIRCNNHSYCFDLKTGDCTLPKGGAPLTVLPAEERGEEVC
                     IRLEW"
     gene            3628..4527
                     /locus_tag="VMZ02_01985"
     CDS             3628..4527
                     /locus_tag="VMZ02_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HUR70726.1"
                     /translation="MDHFSPRPIVRTLVLVLVLTGISARIDQPWAQEKNFLWKAAGEK
                     NTVYILGSIHLLKRDRAVLKPIVQEVFTDSKRLVLEIDLLKEGPEKFQQLLMQKGIDG
                     DGKQLQQQLSPQTYKLAAERADDLGIDLKLLAPFKPWVVALTMVVMQLQKLGYDPNLG
                     IDHQLARRAKQASKPVAGLETAEFQIDIFSRLTAPEQDMFLRQSLLEMDQLEKSVDDV
                     VAVWENGDIARGEQLFLESMLAYPELKAKVLDERNRNWIGQIERFLKEDEDILVVVGA
                     AHLVGKEGVIELLKARGHKLEQM"
     gene            4527..>5011
                     /locus_tag="VMZ02_01990"
     CDS             4527..>5011
                     /locus_tag="VMZ02_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUR70727.1"
                     /translation="MTGRNQQLESIRVEDWMTESVLAVDTYDSIAIARQLMAKHRINQ
                     LPVLDNDHLVGIVTDRDIRDAYPTSMMIDQTQAIDSFAEKVTVEEVMTHDVLIVRPQT
                     PLSAAVGLLRKHRIGALPVVQEKNLVGIITRSDILDYVLDGGPKRKRLATRPVAKAKK
                     KR"
BASE COUNT         1145 a         1313 c         1458 g         1095 t
ORIGIN      
        1 caagcaattc cgtgccgatc ttagttagaa gtattaattc ccggctgcat ggcggagtga
       61 ttgtgctcga cgccgtttgc atgctattgg cttcgctcga ttatcgtcga tcgattacaa
      121 taaaagcgtt cggaagagaa cgtgttatga ttaaggagtt ttgaaccatt tgttatggac
      181 gtcatggcag cggaattgaa aacttcggcc cgcaggtggt tcgtctgggc cgccgcactg
      241 gtgattacgc taggttcgct cgcgggtatt cggacattgc tgagttggtg caacgatagc
      301 caccgagtgg ttgagttact gaaccggctc gaaagcgaag tcaattacct cggcgcattg
      361 aagtgggagt cgacggcgcg caagggcgtt tccgaagaag cgcacgagtt gcagttttcg
      421 actcagcaag agatctctca ggttctagac gatcttgatc gcctggtcag taatggcggc
      481 atcaccgaga ttcgtaaact ctatcaggac tatgttcgct ctagtgaagt tcagttccgg
      541 ctaatcgaac gcgggcgtta cgtcgaggct cagcgagttg accaagacaa gatcgatccg
      601 atgtataacg ctttgatcgg tttgatgaat ttgatgcgcg aacgccacac gcagtcggcg
      661 catcagaaga tgctgttggt cgatttcggt atttccgcgg tgctgctatt ggcagcggtc
      721 tccgtcggta tgttgttttc gcgattccaa caaggcctcc gaataaagca tgtgctactc
      781 gtcgagcaaa atgcgctgcg caaaagtgag agtcgatttc gctccctggt gcaaaactct
      841 tccgacgtga tcgccattct caatccgctg ccggcaacgt tcatattcct cagtgactct
      901 gtgcgccgga tcttgggcta ccggcccgat caattgatcg gtaccgatat cggcaagatg
      961 atacatcctg gcgatagcgc gacgctgcag cgatttcttg ccaattgcgc ttacagcaac
     1021 ggcctgactc acgcagccga gctgcgaatg cgccgcagcg acaaccagtg gacaattgtc
     1081 gagatgttcg gcgacaatcg cgtcaacgat ccggatatca gcggcatcgt catcaacttt
     1141 cgcgacattt cggaaacgcg ccagatcgag gcggcgctga ctcaagaagg ctacgaattc
     1201 gatccggcgg agaaaaaact gcactgacgc gcgcttacct tgtttgcggc tcgatcgcgc
     1261 gaaacgaagt caaactccat cgctaagttc tgcgccgact ccggtattct tcctgggtca
     1321 gcgatggaac ataacaaatt catccgaggt ctcgcatgag acgtacatct ctctatcggc
     1381 gcgaacaaat catctgtctc cggttaatct ttgtccgtgg tttgacactg ccgcgcctgt
     1441 cgtcgtatcg ttaaagtcaa tgattagctc tcttattgtc gcttccaaac gaggaggatt
     1501 gcatggctta tgatttggta attcgcggcg gacgcgtagt cgatggctcg gggttgccgt
     1561 cttatgtcgc cgacgtcgct gtcaaggacg gcaaggttgc ggaaattggt aaagttaagg
     1621 atgccgcggc gaagaccatc gatgccggcg gtctcgccgt gtcgccggga tttattgatc
     1681 atcatacgca tcttgacgcg caaatgctct gggaccctta tggcacctgc gagccgcagc
     1741 atggcgtgac ctccgtcgtg atgggcaact gcggattgtc gctggcgccg gtccgccaag
     1801 ggatcgaaga cgccattgtc aaaagctttg tgcgtgtcga agcgattccg cgcatcgcgc
     1861 tcgaacaggg cgtgaaatgg aattggcata gttacggcga gtatctcgac aatcttgagg
     1921 gcaagctcgg catcaacgtc ggaggcttgg ttggtcatat cgcggttcga catcacgcca
     1981 tgggggaaga agcggtggag cgcaaggcca ccggcacgga agtgcaaaag atgaagggcc
     2041 tggtgctcga cgcgatggaa ggcggcgcct tgggcatgtc gaccaaccgc aacgaccgcc
     2101 atatgcgcga agacggaaag ccggtgtcga gccgcctggc ggacgacgat gagttatttg
     2161 cgctctgcga tgtgctcggc gaacgcaacg ccggcgttat cgagacaatt ctcggccgca
     2221 acaaggtcga gcattttgcc tggtaccatg aattggccaa acgcacgcaa cggccgatcc
     2281 tatggcaaag cctgcaacat cgctggtccg agcctaatct atggcgggag cagctcgaag
     2341 ccgtcgaacc gattttcaaa gccggctacc aggcctatgg tttgacccac acggtaccgc
     2401 tggtgcgtca tttcacttta aaagattgcc agatcttcga cgagtttccg acctggaaaa
     2461 atttgatgtt cctgccggtg gaggtgcgca agcaggcatt tgccgatgcc gacacgcgga
     2521 ccaagttgca gcatgagatc gataatttgc gcttgaccaa ctatcacaaa cgctgggata
     2581 tccccaaagt cgaaaagtgt ttcaagccgg agaatcaaaa atatgtcggc aaaaccatcg
     2641 cagagatggc ggccatgcgc aatcaagcgc ccatcgatgc tttcctcgat ctcgcgctcg
     2701 acgaaaactt ggaaaccatt ttctggaatg ctaacaacgg cggcgattgg aacgccatgg
     2761 gcgaaattct ccgcagcccc tatgtcttga tcggtacttc ggatgccggc gcccatgtgc
     2821 tgttcggcgc tgatttcggc tacggcacga ttcttttggg cctgtgggtg cgcgagcgcc
     2881 aagtgatgtc gctggagcag gcgattcaca aactgacctt tcatcccgcg tcgatcttcg
     2941 gcctgcaggg ccgcggctta ctgcggccgg gctatgccgc agatatcgcg atcttcgatc
     3001 ccaaaaccgt taacgcagag gagccagtct gggccgatga ttttcctgcc aacagtaaac
     3061 gcatggtgca gcaagcgact ggcatgcatt acaccatcgt caatggcacc gtgatcaacg
     3121 atcacggaca catgaccggc gacctgccgg gccaggtgat gcgcggcccg ctttaccgtc
     3181 agcaaaaggc ggctgcgtaa tatgggagtg agttatattc cggttgcccg tatcggagaa
     3241 ctcagtgcgg gggccatgaa ggaagttgac ctcggcggac gccaagtact ggtggcgttc
     3301 gtcgagggca aatactatgc attctcgcgt cactgtcccc acgaggaggc ggatttgaag
     3361 gaggcgggac agcttctcgg caatacccag attcgctgca acaatcatag ttactgtttc
     3421 gatcttaaaa ccggcgactg cacgctgccc aaaggcggcg cgccgttgac cgtacttccc
     3481 gccgaagaac ggggcgaaga ggtctgcatt cgcctcgagt ggtgaattcg cgcttggcga
     3541 ttgggtctcg ctgctcgtca gcgtgcgagc gagtgttcgt cggggctcgc attcgataac
     3601 accgccatca cggccggcac gatgcccatg gatcatttta gtccgcgccc catcgttcga
     3661 actttagtgc ttgtgctggt gcttaccggc atcagcgccc gtatagatca gccttgggcc
     3721 caggagaaaa acttcctctg gaaggctgcg ggcgagaaaa acacggtcta cattctcggt
     3781 tcgatccatc tgctcaagcg cgaccgcgcc gtgctgaaac cgatcgttca ggaagttttc
     3841 accgatagta aacgtttggt gttggaaatc gacctgctca aagaggggcc ggagaagttt
     3901 cagcaattgt tgatgcagaa gggcatcgat ggcgacggca agcagctgca gcagcagtta
     3961 tcgccgcaaa cgtacaaatt agccgctgag cgggccgacg acttgggcat cgatctcaag
     4021 ctgttggcgc cgtttaaacc ttgggtcgtc gcattgacca tggtggtgat gcagttgcag
     4081 aagttaggct acgatccgaa tctaggcatc gatcatcagc tagctcggcg cgccaagcag
     4141 gcgagtaagc cggttgccgg cttggaaact gccgaattcc aaatcgatat attcagccgt
     4201 ctgactgctc cggagcagga catgttcctc cgccagtcgc tgctcgaaat ggaccaactc
     4261 gagaaatccg tcgacgatgt ggtggcggtg tgggagaacg gcgatatcgc gcgaggcgag
     4321 cagctgtttc tcgaaagcat gcttgcctat ccagagttga aagccaaagt actcgacgag
     4381 cgcaatcgta attggatagg gcaaatcgaa cgattcctca aagaagacga agacattctc
     4441 gtggtggtcg gcgccgccca tttggtcggt aaagaagggg tcatcgagtt actgaaggcg
     4501 cgcggacaca agttggagca aatgtaatga ctggacgaaa tcagcagctt gaatcgattc
     4561 gggtggagga ttggatgacc gaaagcgttt tagcggtcga tacctacgac agtattgcca
     4621 tagcccggca gctcatggcg aagcatcgaa tcaaccagtt gccggtgctc gacaatgacc
     4681 atcttgtcgg tatcgtcacc gaccgagaca ttcgcgatgc ctacccgacc agcatgatga
     4741 tcgatcagac ccaggcgatc gacagctttg ccgaaaaagt caccgtcgag gaagtaatga
     4801 cgcacgacgt cttgatcgtg cggccgcaaa cgccgttgtc ggcggcggtg ggattgttgc
     4861 gcaaacatcg aatcggcgct ttgccggtgg tgcaagaaaa aaacctcgtc gggattatca
     4921 cgcgcagtga tatcctggat tacgtgctgg atggcgggcc gaagcgcaaa cggctggcca
     4981 caagaccggt cgcgaaagcc aaaaagaagc g
//
LOCUS       DATLCH010000070         4984 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10551496, whole genome shotgun sequence.
ACCESSION   DATLCH010000070 DATLCH010000000
VERSION     DATLCH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10551496"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VMZ02_01995"
     CDS             complement(<1..236)
                     /locus_tag="VMZ02_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70728.1"
                     /translation="MPAEPSVNSKEGGHFLPRFKAVRNPDSVPKPRRLERSGNVLYFR
                     DRVAAPDVGGGRGLRRALRIDDDDLLSKIYEQHK"
     gene            474..713
                     /locus_tag="VMZ02_02000"
     CDS             474..713
                     /locus_tag="VMZ02_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70729.1"
                     /translation="MKDSAKYAKIVEWSEEDQCYVGSAPGLIFAGCHGAEEQEVFQEL
                     CQIVEEAIEIYRKDGKPLPPPTSGRDFATKMQNVA"
     gene            complement(876..1781)
                     /locus_tag="VMZ02_02005"
     CDS             complement(876..1781)
                     /locus_tag="VMZ02_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70730.1"
                     /translation="MLAIEFYASPTRAENIKIGLPSVTITAMPFFVAREHGFFQQEGL
                     NAEMVVMPASLNIKVLLAGDIQYAATMGSAVAAAIRGINVRIVMLFVDRPLQDLVGAA
                     NIATISELKGKVLAISSRGGLQDIIMRRILTQSKIDPSQVNIITIPGQTAMISALKTN
                     RIGAAMLNPPFNFLAYREGLTNLGFSGNFVRLPSTGVATLGETLERSPDQVRRLTRAL
                     ARARTFAKENKVKVFPTLKRALRIDDDDLLSKIYEQHKQVETADGRVDAQLMTDTIRD
                     ARQTEGITKEIPVNQVFDFSYLPAR"
     gene            complement(1894..2988)
                     /locus_tag="VMZ02_02010"
     CDS             complement(1894..2988)
                     /locus_tag="VMZ02_02010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70731.1"
                     /translation="MNLFGTVDADGHLEENHIDWKARLPAKYQSQAPERRDSGNGQLR
                     TIIEGKAWPRPSGLGIGVGGPYSRPHPRRPGMTDPQARLLDMDDENIDVAVLFGAGLA
                     GTLPALDDAGLAAALATARNSWLAEYCAADPQRIKGTAALAQQDIDASVTELRRSVEQ
                     LGMVGASIFPNVKGRHLGDPYYFPLYAEAEKLNIPICVHMFLGRYGSDATGTERVDKF
                     FYSHLFGHPFEQMIALSVILGEGVLDRFPKLRFVFLESGCGWLPYWVERMDEHYEVLG
                     VQLPDMNAAPSKLLERGQLYFSFEADESELTHVIEVFGDDWLVFASDYSHFDSHWPGA
                     SEPIVNHPKLSAATKRKILNDNARKLYPLD"
     gene            complement(3050..4081)
                     /locus_tag="VMZ02_02015"
     CDS             complement(3050..4081)
                     /locus_tag="VMZ02_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70732.1"
                     /translation="MLNLKLGVTKNPRFEPLIDGSVKSDKAALNIHVTTPPELFYKNL
                     KNDEFDVFEMSLSEFLITREQAKGDRWQWAALPIFPAKAFVWMTLFVNTGAGIQGLCD
                     LKGKRVGIPDYVMTAGLWFRIILRELCGIEPKDISWYIGRVQDLSHGGLLGMDSRPPA
                     GISTTWLTKEQTFDVMLDKGEIDAAYGFAPRHDAKLMTLNIDRYGGTPLEGNPRLRKL
                     FSDGGLSIVEQFHKKTGIVPANHVIVAQRRLLQENAWLPGEIVRLFSESKRIAYERGN
                     YGNPAYLYFEDSDRASQEKMVGNDPFPFGIANNRKMLETLFRNSHAEGLTQRLAKIEE
                     VFFPALLDT"
     gene            4388..>4984
                     /locus_tag="VMZ02_02020"
     CDS             4388..>4984
                     /locus_tag="VMZ02_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUR70733.1"
                     /translation="MKICHYNNNQAGVVVGDKVINFGDAMIKAGLARQGYTMVEIIDA
                     LANNSAAMQIVRDAANSSGGLDVKSVKLLAPIQNPGTLWAAAANYGAHRAEMVQRMGN
                     AERPVINKDDLMAEFFIKTTSSIIGPGDTIILPKRSNLVDFECELCAVIGQRARNVSE
                     ADSLNHVFGYMMCWDISQRDPWGKGMHNTRNIRKGMDTF"
BASE COUNT         1109 a         1288 c         1464 g         1123 t
ORIGIN      
        1 tgtttgtgtt gttcgtagat cttcgacaat aaatcatcgt cgtcgatgcg cagggcgcgc
       61 cgtaagcctc tcccgccgcc gacatccggc gccgcgactc ggtcacgaaa atacagaacg
      121 ttgcctgatc gttctagcct gcgaggcttg ggcaccgagt ctgggtttct gaccgctttg
      181 aaacgcggga gaaaatggcc gccttcctta gagttgacag atggttcagc cggcataatc
      241 tactctcaat atcatggaac agaggctttg ggaccagacg tgccgcctaa gataagggaa
      301 ctcattgctg accttcagca agcaggtttt gtcgatcggg gcggaaaggg gagccaccgt
      361 aattttgttc atcctaaagt cggcaagccg ataactattt ctggttaacc gggcgacgat
      421 gccaaacgtt atcaaattcg agccgtgcag ctcgcggtcg aggagacgaa gaaatgaaag
      481 acagcgcgaa atacgcaaaa attgtcgagt ggtccgaaga agatcaatgt tacgtcggta
      541 gcgcgcctgg cttaattttc gctggctgcc acggcgctga ggaacaagaa gtgttccaag
      601 agctgtgtca gattgtcgaa gaagcgatcg agatctatcg caaagatggt aagccacttc
      661 cgccgccaac atcgggtcgc gacttcgcta cgaagatgca gaacgttgct tgatcgtcgc
      721 accgagaagg cctatgcgtc cagtgcaggt ttgtaacccg cggtgaaacg cggacagcaa
      781 tatccgcgct ccgctaaatc gacttcatcc ctcgacagac aaccaccgac gcgccagggc
      841 atagtgaacc gtcagatcat tcagtcttcg gccgttcaac gggcgggcag ataggaaaaa
      901 tcgaagacct gattaaccgg gatttctttg gtgatgcctt ctgtctgacg ggcgtcgcgg
      961 atcgtgtcgg tcatgagctg ggcatcgacc cgaccatcgg cggtttccac ttgtttgtgt
     1021 tgttcgtaga tcttcgacaa taaatcatcg tcgtcgatgc gcagggcgcg tttgagagtc
     1081 ggaaagactt tgactttatt ttctttcgcg aaagttctcg cgcgcgccag agcgcgggtc
     1141 aagcgccgga cttgatccgg cgaccgttct aaagtctcgc ccaacgttgc cacgccggtg
     1201 ctcggcaggc gcacgaaatt tcccgaaaaa cccaaattgg tcagtccttc gcggtaggcg
     1261 aggaaattaa atggcgggtt taacatggcg gcgccaatgc ggttggtttt caatgcactg
     1321 atcatcgcag tttggccggg gatcgtgatg atattgactt ggctgggatc gatcttcgat
     1381 tgggtaagaa ttcgccgcat gatgatgtcc tggagtccgc cgcgggacga gatcgccaga
     1441 actttgcctt tcagttcaga aatggtggcg atattagctg cgccgacgag atcctgcagt
     1501 ggccgatcga cgaataacat gacgatccgc acgttgatgc cacggatcgc ggcggcgacc
     1561 gcggagccca tggtggcggc gtattgaata tcgccggcaa gcaacacttt gatgttgagt
     1621 gacgctggca tcacgaccat ctcggcgttg aggccttctt gttggaagaa gccgtgttct
     1681 cgggcaacga agaacggcat ggcggtgatg gtcaccgatg gcaggcctat tttgatgttc
     1741 tcggcgcggg tgggagatgc gtaaaattca atcgctaaca agattgcagt aacggtgctg
     1801 agcattcgta gccgataggg gcgagggaac cggaactcgt ggcgtcggcg aattggtttc
     1861 atggttgtca aattctcgtt gccgctatgc tggtcaatcc agtggataca gcttgcgtgc
     1921 gttgtcgttc aatattttcc gtttggttgc ggcactcagt ttcggatgat tgacgatcgg
     1981 ctccgaagcg ccgggccagt ggctgtcgaa gtggctgtag tctgaagcga aaaccagcca
     2041 gtcatcgccg aacacttcga tgacatgggt gagttcgctt tcatccgctt caaaggaaaa
     2101 atagagctgg ccgcgttcga gtagtttgct gggtgctgcg ttcatgtccg gcaactgcac
     2161 gccgagaact tcgtaatgct cgtccattct ctcgacccaa tagggtagcc agccgcagcc
     2221 cgattccaag aatacgaagc gcagcttggg aaaacgatcg agcacacctt cgcccaggat
     2281 caccgacaaa gcgatcatct gctcgaaagg atgaccgaac aggtgcgaat aaaaaaactt
     2341 gtcaacgcgc tcggtaccgg tggcgtcgga gccgtaacgg ccgagaaaca tatgcacgca
     2401 gatcggtatg ttcagcttct ccgcttcagc atagagtgga aagtaataag gatcgccgag
     2461 atgccggcct ttgacgttgg gaaagatcga agcgccgacc atgccgagtt gttcgacgct
     2521 gcggcgcaat tctgtcacgc tcgcatcgat gtcctgctgt gccaaggcag cggtgccttt
     2581 gatgcgctgg ggatccgcgg cgcaatactc ggcgagccaa ctattgcgcg cagtggccag
     2641 ggcggcggcg aggccggcgt cgtccaaggc cggcaaagtt ccggcgagac cggcgccgaa
     2701 gagcacggcg acgtcgatgt tttcgtcatc catgtctagc aagcgcgcct gcggatcggt
     2761 catccccgga cggcgcggat gagggcggct gtacggaccg ccaacgccga tgccgaggcc
     2821 gctgggacgc ggccaggctt tgccttcgat gatcgtgcgc agctggccgt tgccgctgtc
     2881 gcgccgttcc ggcgcctgac tttgatattt cgccggcaga cgcgctttcc aatcgatgtg
     2941 gttttcttcc aagtggccgt cggcgtcgac ggtgccgaat aggttcataa atcctcgctg
     3001 cgctcgtggg gaattacaga ttacaattcc acattccaag ttatcaggcc taagtatcga
     3061 gcagggccgg gaagaaaact tcttcgatct tggctaggcg ctgggttagg ccttctgcgt
     3121 gggagttgcg aaagagggtt tcgagcattt tgcgattgtt ggcgatgccg aacggaaacg
     3181 gatcgttgcc gaccattttt tcttggctgg cccgatcgct gtcttcgaaa tacaggtaag
     3241 ctgggttgcc gtagttgccg cgctcgtagg caatgcgctt gctctcgctg aacagtcgga
     3301 cgatttcgcc gggcagccaa gcattctctt ggagcaggcg acgctgcgcg acgatgacgt
     3361 gattggccgg tacaatgccg gtttttttat gaaactgttc gacgatcgac aaaccgccgt
     3421 cgctaaacaa ttttcttagc cgcggattgc cttccagcgg tgtgccgccg tagcggtcga
     3481 tatttaatgt catcagcttg gcgtcatggc gcggtgcaaa gccgtaggcc gcatcgattt
     3541 cgcctttgtc gagcataaca tcgaaggtct gctctttagt cagccatgtt gtcgatatgc
     3601 cagctggcgg acggctgtcc atgccgagta atccgccgtg gctcaagtcc tgaacccgcc
     3661 cgatgtacca ggagatgtct ttgggctcaa tgccgcagag ctcgcgcaga ataatccgaa
     3721 accacaaacc ggcggtcatg acataatcag gaatgccgac gcgtttgccc ttgaggtcgc
     3781 acaggccttg aataccggct cccgtgttga cgaatagggt catccagaca aatgctttgg
     3841 caggaaagat cggcaaagcc gcccattgcc aacggtcgcc tttggcctgc tcccgggtga
     3901 tgagaaattc cgacagcgac atttcgaaga cgtcgaactc gtcgttcttg agatttttat
     3961 aaaacagttc cggcggcgtg gtgacatgga tgttcaaagc cgctttgtcc gatttgacgc
     4021 tgccgtcgat caacggctcg aagcgcgggt tcttggtgac acctagtttc aggttcaaca
     4081 tgcaaagctc gtttcgtaag aagatgtctc attcatacgc tgaggtgcaa tggaaggtca
     4141 aactctgccg aaggtgaaag gtctgcaaca cgaagcgcac gaagctcgga atcttagttg
     4201 ctcaagcgcg cgcgcttgaa aataatttac ttaggcctgg cgtcaccggc ttgtcaataa
     4261 attttgcgag catcgctaga gatcggcgac ccagttatgt cgatactggc gatgcatcat
     4321 tggacattaa ggggtgcttc aggtaagaga ggaaatccta cgttcgcaaa aattcggagg
     4381 gcttggaatg aaaatctgtc attacaacaa caatcaagcc ggcgtcgttg tcggcgataa
     4441 agtgatcaac ttcggtgatg ccatgatcaa agctggactg gcgcgccaag gctatacgat
     4501 ggtcgaaatc atcgacgccc tggcgaacaa ttcggcggcg atgcagatcg tgcgtgacgc
     4561 ggccaatagc tcgggtggtc tcgacgtgaa atcggtgaaa cttctggcgc cgatccaaaa
     4621 cccgggcacg ctgtgggcag cggcggcaaa ttacggcgcg catcgcgccg aaatggtcca
     4681 acgcatgggc aatgccgaac gccccgtcat caacaaagat gatttgatgg ccgaattttt
     4741 catcaagacg acatcgtcga ttatcgggcc cggcgatacg atcattttgc ccaagcgctc
     4801 gaacctggtg gatttcgagt gcgagctttg tgccgtcatc ggccagcgcg cgcgcaatgt
     4861 cagcgaagcg gattcgctga accatgtttt cggctatatg atgtgctggg acatcagtca
     4921 gcgcgatccc tggggcaagg gcatgcacaa tacgcgcaat attcgcaaag gcatggatac
     4981 tttc
//
LOCUS       DATLCH010000071         8509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3958277, whole genome shotgun sequence.
ACCESSION   DATLCH010000071 DATLCH010000000
VERSION     DATLCH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3958277"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VMZ02_02025"
     CDS             <1..445
                     /locus_tag="VMZ02_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70734.1"
                     /translation="VGAAGEKVVPLEKFFILPSMDYRRENVLSPSEIVTEIFVPIPKP
                     GSKGFHHKLRERLAWDHAIVAVDTVVESSSGVVRHASVVMGGVAPIPWRAVKAEEFLR
                     GKRLDDAIAKQAGELALDGAKPLKDNIYKVKIAQDLIQRGLLASV"
     gene            595..1362
                     /locus_tag="VMZ02_02030"
     CDS             595..1362
                     /locus_tag="VMZ02_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70735.1"
                     /translation="MLAEVFKNRYIIASAAFFPFAVMVILLAGGRDNFRLPWLKGSAN
                     PELSGPDNLALMRESLAQNRRELAQFEREQSRLRAEVIRQRKLLQESAITKEQVQQTE
                     QAFVVALKRVHAMRYTVTETDIAITEAILGEKVLRMPALPIGGFSQTAGLTRFNGPAK
                     WSIAQAPTVEKFFSQSFGRRLPITALGQSDTHNRLGFDHRASMDVGVHPDSSEGQALV
                     NYLRKSGIPFLAFRTAVPGSATGPHIHIGPPSGRLTH"
     gene            complement(1596..2387)
                     /locus_tag="VMZ02_02035"
     CDS             complement(1596..2387)
                     /locus_tag="VMZ02_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HUR70736.1"
                     /translation="MTPFNLVKICAVLAAWLILPGKSLAADGNSLPDTIEKIKPAVVA
                     IGTFQKTRRPPSIFRGTGFVVADGLHVITNAHVLPEKLDAEKREVIAVFAGNPNQIEI
                     REATKAAEDREHDLAVLKLTGRPLATVILGGAQRVREGEVYAFTGFPIGMVLGLNAVT
                     HRGIVSAISPIAIPQLSAQLLEKNVVARLTAPYNVFQLDATAYPGNSGSPLYDPVTGQ
                     VVGIINKVFVQESKETVIQKPSGISYAIPVNHIRDLLRKLGIEVN"
     gene            2566..3312
                     /locus_tag="VMZ02_02040"
     CDS             2566..3312
                     /locus_tag="VMZ02_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70737.1"
                     /translation="MRTRPRALAMMFSLGFLLPIAGFSQGPQRNQRSAAVDSELARSR
                     AEVLQKTKETRAGAERLLALHEAELKRVTEEYEQRRELYYQGLISRVDVIQAEERVAK
                     TTLLIRDDKRFLAETDTALTEFTMRDELFRLPGLALGGYSETASLLRFNGAAGFTLAE
                     VAKIERFFTSTFGRGLPISALGQTSTHDRMRFDHRNAVDVALHPESAEGQSVLNFLRQ
                     AGIPFIAFRAAVPGAATGAHIHIGKPSPRL"
     gene            3465..4100
                     /locus_tag="VMZ02_02045"
     CDS             3465..4100
                     /locus_tag="VMZ02_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70738.1"
                     /translation="MNRRPQEMASYAGVFVFPGGRVERTDWSSAMLNLVRGISPEDAQ
                     RKLGNIAGPEICLGYWVAAARELFEEAGIHFFVPRCNLPTIESSNLAERLAQKRADLQ
                     DGKFDLQTLMAAEQMYCDVGRLSYFFHRVTPEHYPVRFDTRFYLAILPPGQEPLHSSE
                     EVSESIWVTPRAALDYSQTGNFPMMPPTIAVLRTLADHESWQSLCDTFHLS"
     gene            4391..6118
                     /gene="glgP"
                     /locus_tag="VMZ02_02050"
     CDS             4391..6118
                     /gene="glgP"
                     /locus_tag="VMZ02_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HUR70739.1"
                     /translation="MPASVPQEGMIAYFSMDVAVDSDIPTYSGGLGVLAGDMLRSVAD
                     LSLPMVALSLLHHKGYFDQRLDSHGNQMESPVKWSPERHLTRLPEKVTLNIGGRSVQI
                     GAWRYLFAGVTGHTVPLIFLDADLPENDASDRSLTDYLYGGDERYRLCQEVILGIGGV
                     LMLRALGFKDVHVFHMNEGHSALATLALLEEQTGPGGERRYSNSEIESVRRQCVFTTH
                     TPVPAGHDRFPADLVCQVLGDTRAATLRDLQVLNGSLNMTELALRFSGYVNGVSMRHG
                     EVSRNMFPNHHIEAITNGVHATTWTAAPMAALFDRTLPKWREDNCFLRYTVGIPVSEI
                     RQAHALAKLDLLRQVRWLTGIQFDEKIFTLGFARRATSYKRGDLLFTDIEQLKNVSER
                     VGPIQLVYAGKAHPRDDGGKAIIRRIFEAAASLANSVRVVYLENYDMALGKLLCSGVD
                     VWLNTPMRPQEASGTSGMKAALNGVPSFSVLDGWWIEGHIEGVTGWSIGDAVNADNDS
                     VIEARSLYDKLETVILPLYYQEPDKFAQVMRSAIALNGSFFNTQRMLFQYVRNAYAPA
                     RNAAARDFD"
     gene            complement(6139..7017)
                     /locus_tag="VMZ02_02055"
     CDS             complement(6139..7017)
                     /locus_tag="VMZ02_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR70740.1"
                     /translation="MSIQAESSGQSGAASFFTVLLFFLTGCGLENRLIFHPSTEILRT
                     PRDVGLGYQDVYFTTVDNVRLNGWFIPHPKAGTTMIWFHGNAGNIGDRVANIKLLHDK
                     TCINIFIFDYRGYGRSPGSSSERTTYLDGEAAMNFVRSQLSIEGQNLVIFGRSLGAAV
                     AAEMATRYDSRAVILESPFISIREMAKVALPGWPVGPLLSTKFDVIDKVARITAPMLV
                     LHGDHDEIIPFEHGQKVFAAAHEPKQFYPIAGAGHNDTFVAGGNSYYDRLRSFIENPH
                     QNYLESCKNGCCSAGA"
     gene            7262..7837
                     /locus_tag="VMZ02_02060"
     CDS             7262..7837
                     /locus_tag="VMZ02_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70741.1"
                     /translation="MPEDDSITDRSLQGYFLQVVRQTFWQVGLYDATVAEYIAKVLAE
                     FALADNLYKIRDRGGRKIDSVVELLAKAPVVPADEADILRQRTLRKYVGDYALFMSGV
                     FRTHIDGKGSLGYYLQEGSRSYWTVSELDLALYRTGYYLYQELSTKFEYYSGALDFMR
                     KSYFAAAPGEDPFAGFLRQIKGWMKIHVSEN"
     gene            7887..>8509
                     /locus_tag="VMZ02_02065"
     CDS             7887..>8509
                     /locus_tag="VMZ02_02065"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HUR70742.1"
                     /translation="MKLLRTVVLSTVLVLPLLAAWFFYNINYSRPASGAYPIEVRVES
                     GDSFATVTRKLREQQVISNGLFFSLWARFKGSEKKVHQGLYRFETSVTPSEVLERLVT
                     GKGIFYTVTIPEGLTVKEIAELLDKMQIADGEKFLAVAADPAVLGALGLGDRGLEGYL
                     FPSTYHFIPATPERDIIGAMTEQFRKSSQPLLAKASMAQLTTHELLTL"
BASE COUNT         1853 a         2264 c         2383 g         2009 t
ORIGIN      
        1 tgtcggggcg gcaggcgaga aggttgttcc gctggagaag ttttttattc tgccgtcgat
       61 ggattaccgg cgagaaaatg ttctcagccc tagtgagatc gtcaccgaaa ttttcgtgcc
      121 catacccaaa cccgggagca agggattcca ccacaaattg cgcgaacgtt tggcttggga
      181 ccacgccatc gtcgcggtcg ataccgtcgt cgaaagcagc agtggcgtag tgcgtcatgc
      241 cagtgtagtc atgggcggcg tggcgccgat tccctggcgc gcggtcaagg ccgaagaatt
      301 tttgcgtggt aaacggctag acgatgccat agccaaacag gctggtgagc tcgccctcga
      361 cggcgccaag ccgctgaagg acaatatcta caaggtcaag attgctcagg atctgattca
      421 gcggggtttg cttgcctcag tctaaaactc gactcagtcg agtgtgaact cttgcacggc
      481 tcgccgtcgg gattgtttta gacaatctct taactccacg cggggccaag ccgaaggcat
      541 agttgacaat tgtggcttga cctgtccggg aggacccctt aaacttcggg cctcatgctc
      601 gccgaagttt tcaagaatcg ctatattatc gccagcgcgg cgtttttccc cttcgctgtg
      661 atggtgattc ttcttgctgg cggacgggac aatttccgtc tgccttggct caagggaagt
      721 gctaatcccg agctttcggg ccctgataac ttagcgttga tgagggaatc gcttgcgcag
      781 aaccggcgcg agcttgcgca attcgagcgc gaacagtcga gattgcgtgc tgaggtcatt
      841 cgccagcgca agctcttgca ggaaagcgcg attaccaaag agcaggtgca acaaaccgag
      901 caggcgttcg tggtcgcgct aaagcgcgtc cacgcgatgc gttacaccgt gactgagacc
      961 gacatcgcca tcacggaagc tattcttggc gaaaaagttc tgcgcatgcc ggccttgccg
     1021 atcggcggct ttagccagac cgcgggtctc acccgtttca atggaccggc gaagtggtcg
     1081 atcgcccaag cgccgacggt cgagaaattt ttttcccaat ccttcggccg ccgattgccg
     1141 atcacggctc tgggccagtc ggatacccat aatcgcttgg gcttcgatca tcgcgcctca
     1201 atggatgtgg gagtacatcc cgacagcagc gaagggcaag cactagtcaa ttatctgcgc
     1261 aagtcgggca ttccctttct cgccttccgc accgccgtgc ctggttccgc cacagggccg
     1321 catattcata tcggccctcc ttctggacgg ctcacccatt agtcgggatc gcctcctcgt
     1381 cctatgcgag ccgcgagaac gcgccaactg ttgaattaca gcggttagcg ctggtgattt
     1441 gccgttactc ctttattaga cagaacgacg cagcattaat cggcggttgc cgcagcaatg
     1501 gtggtgcttg gctctaacgt tctgaatacg agcataacag agctctcagg ctcggacgca
     1561 gcttagaatc acgcggccgt cccatcgcgc agtagctaat tgacttcaat tcccaacttt
     1621 cgtagcaaat ctctaatgtg attgaccgga atggcgtagg aaataccgct cggtttctga
     1681 ataacggttt ccttactttc ttgcacgaac actttgttga ttataccaac gacttggccg
     1741 gtcaccggat cgtaaagtgg actgccactg ttgccaggat aagccgtggc gtctagttgg
     1801 aatacattgt acggcgcggt gagtcgcgcg acgacattct tctccaatag ctgagcggag
     1861 agctggggga tcgcgatcgg cgagatcgcc gaaacaatgc cccggtgtgt gactgcattg
     1921 aggcccaata ccatgccgat tggaaagccg gtgaaagcgt aaacctcgcc ctcgcgcact
     1981 cgttgcgcac cgcccaaaat caccgtcgcg agcgggcgtc cagtcagctt cagtacggca
     2041 agatcatgtt ctcgatcttc agccgctttg gtagcttcac gaatttcgat ttgattgggg
     2101 ttgccggcaa aaactgcgat tacttcccgt ttttcggcgt cgagcttttc cggcaacaca
     2161 tgcgcgtttg tgatgacgtg gaggccgtcg gcgacaacga atcccgtgcc gcggaaaatt
     2221 gaaggcggac gacgcgtttt ctgaaacgtg ccgatggcca ccacagccgg cttaattttc
     2281 tcgatggtgt caggcagaga attgccgtcg gcggctagac ttttacctgg aagaattaac
     2341 caggctgcca atacggcgca gatttttacc agattaaacg gagtcatctt tggatcaaac
     2401 cttgcgcacc gagtgctagc cgaactggcg ctatcgtgac tgctgtatta tcgtggccat
     2461 tccgcgcgcc ataatagcct caagaatggg tggcgcgcaa tctggcttga cgcgaagtgg
     2521 cttaattcat agagtttcgc acatgattca aactgtgttg atctaatgag aacccgccca
     2581 cgagccttgg cgatgatgtt cagcttggga tttctgctgc cgattgcggg attttcccag
     2641 gggccgcaga gaaaccagcg gtcagccgca gttgattccg aacttgcccg ctcgcgcgcc
     2701 gaggtgctgc aaaaaaccaa agagacccgt gccggcgcag aaaggctttt ggcgcttcat
     2761 gaggcggaac tcaaacgcgt gactgaggaa tatgagcagc ggcgcgagct ttattaccaa
     2821 ggtttaattt ctcgcgtgga cgtgattcag gcggaagaac gggtggcgaa gacgacgtta
     2881 ctgatacggg atgacaaacg ttttcttgcc gaaaccgaca ccgcgctcac cgagtttacc
     2941 atgcgcgacg aactgttccg gttgcccgga ctggctctcg gcggctatag cgaaactgct
     3001 agtttgctgc gcttcaatgg tgcggccgga tttacgttgg ctgaggttgc caagatcgaa
     3061 agatttttta ctagcacttt tggccgcggt ctgccgatca gtgctctcgg tcagacatcg
     3121 acccacgacc gcatgagatt cgatcaccgt aatgcggtcg atgtcgcgct tcaccctgaa
     3181 agcgcggagg gccagtccgt actcaacttt ttacgtcaag ccgggattcc gttcattgcg
     3241 tttcgcgccg cggtacccgg tgccgccacc ggggcgcata ttcatatcgg caagccgtcg
     3301 ccgcgcttgt aggcaagctt ttcccgttcg gggcgatcca atgagttgca gccaagactc
     3361 gttttagcta agtcacttaa agcatgtcac agccatctat ccctatgccc gcttcgacag
     3421 tcgttttggt tcgaccgagt gaagatggcg ctttccagat tttcatgaac cgtcggccgc
     3481 aagaaatggc cagctacgcc ggtgtctttg tttttcccgg cggacgggtc gaacgcacag
     3541 attggtcttc agctatgctg aatttggtcc gcgggatttc accggaggac gcgcagcgca
     3601 aactcggcaa catcgccggt ccggagattt gtctgggcta ttgggtcgcc gcggcgcgcg
     3661 aattatttga agaggcaggc atccattttt ttgtgccgcg atgtaacttg ccgacgatcg
     3721 aatcgtccaa tcttgctgaa cggttggcgc aaaagcgtgc ggatctgcag gacggcaagt
     3781 tcgacttgca gacactaatg gcggcagagc aaatgtattg tgatgtcggc cggctcagct
     3841 atttttttca ccgagttacg ccggagcatt acccggtgcg ctttgacacc cgcttctatc
     3901 tagcgatttt gccgccggga caagagccct tgcatagctc ggaagaggtg tccgagagta
     3961 tttgggtgac gccaagggcg gcgctcgact atagccaaac cggtaatttc ccgatgatgc
     4021 caccgaccat agcagttttg cgtacactcg ccgatcacga atcttggcag tcgctttgtg
     4081 acacctttca tctgagttag cggttgacta aacctgccaa agaccgtcat cagacgctcg
     4141 tactaagtac agactctgct acccgatctt cctggtttct ccgcttggag gtttggacac
     4201 taccgccgcc cgcgcacggg cgaagcaatc gacaatcgga ctagattgct ccgcggagaa
     4261 ttgcccaata attgcaataa tgttatattg cttgaagtcc aaagaattag ctaagaactg
     4321 actttacgac acttcagcac aaatactccg tcagccattt tatcggtaac cccgtccaat
     4381 gagagttgat atgccagctt cagtacccca ggaggggatg atcgcctatt tctccatgga
     4441 cgtcgcagtg gattccgata ttccgactta tagcggcggt ttaggggttc tggccggcga
     4501 tatgttacgg tcggtggccg acttatctct cccgatggtg gctttgtcgt tgctgcatca
     4561 taaaggctat ttcgatcagc ggctcgactc gcacggcaac caaatggaga gtccagtaaa
     4621 atggtcgccg gagcgacacc tgacacggtt accggagaag gtgacgctaa acattggcgg
     4681 ccggtcagta cagatcggcg cttggaggta tctgttcgcg ggcgtcaccg gccacaccgt
     4741 gcctttaata tttctcgatg ccgacctgcc ggaaaacgat gccagcgacc gcagtctaac
     4801 cgattatcta tatggtggag atgaacggta tcgcctatgc caggaagtga tcctggggat
     4861 cggcggcgtg ctcatgctgc gggctctcgg attcaaagac gttcacgtat ttcacatgaa
     4921 cgagggtcat tcggcgctgg cgactctggc gctactggaa gaacagaccg gcccgggggg
     4981 tgaacggcgg tattcgaaca gcgaaatcga gtcggtacgt cgccagtgtg tttttacgac
     5041 gcatactccg gttccggctg gccatgatcg tttcccagcc gacttggtct gccaggtgtt
     5101 gggcgacacc cgcgcggcta cgctcagaga tcttcaggtg ctgaacggct cgcttaacat
     5161 gactgagctg gcgttgcgtt tttcgggata tgtcaacggc gtgtcaatgc gccatggcga
     5221 agtctcccgc aacatgtttc ccaatcatca catcgaggcc atcaccaatg gagttcatgc
     5281 gaccacctgg acggccgcgc ccatggcggc gctatttgac cggacgctgc cgaaatggcg
     5341 tgaagacaat tgttttctgc gttacaccgt cggtattcct gtgagcgaga ttcgtcaagc
     5401 ccatgctctg gcgaaattag atctgctgcg tcaagtgcgc tggctcacgg gaattcagtt
     5461 cgacgagaaa attttcacgc tgggatttgc ccgccgcgcc actagctata agcgcggcga
     5521 tttgttgttc acggatatcg agcagctgaa aaatgttagt gagcgcgtcg gaccgattca
     5581 gcttgtctat gccggtaagg ctcatccccg tgatgacggt ggtaaagcga tcattcggcg
     5641 catttttgaa gccgccgcct cgctggccaa cagcgtgcgc gtcgtttacc tcgagaacta
     5701 tgacatggct ctcggcaaac tactgtgctc tggcgtggat gtttggctca atacgccgat
     5761 gcgcccccag gaagcttcgg gtaccagcgg catgaaggcg gccctgaacg gcgtgcctag
     5821 ctttagcgtg ctcgacggct ggtggatcga agggcatatc gaaggggtga ctgggtggtc
     5881 catcggcgat gcagttaacg cggacaacga ttccgtcatc gaggcccggt ccctatacga
     5941 caaacttgaa actgtgattt tgccgctcta ttaccaggag cctgataaat ttgcccaagt
     6001 gatgcgatcg gcgatcgctc tcaacggatc attcttcaac acccagcgca tgctgttcca
     6061 gtatgtgcgt aatgcttatg cgcctgcacg caatgccgcg gccagggact tcgactaatc
     6121 ctgcgagact ccattaaatt acgccccggc gctgcaacat ccgttcttac aagattctaa
     6181 atagttttgg tgcgggtttt ctataaagct gcggaggcgg tcgtagtagc tgttgccgcc
     6241 tgcgacgaaa gtgtcgttgt ggccagcgcc cgcgatcgga taaaattgct taggttcgtg
     6301 ggctgcggcg aaaacttttt gtccatgctc gaaaggaatg atctcgtcgt gatcaccatg
     6361 aagcacgagc attggcgcag tgattctggc gactttatcg atcacgtcaa atttggtgct
     6421 taacaaaggg ccgaccggcc acccggggag ggctacctta gccatttctc taatcgaaat
     6481 aaaagggctt tccaagatga cggcccgact gtcatagcgg gtcgccatct cagcggcgac
     6541 cgctgcgcct aacgaacggc cgaagatcac caggttttga ccctctattg agagttgaga
     6601 gcggacaaag ttcattgccg cttcgccatc aagataagtg gttctttccg acgaactacc
     6661 ggggctgcgg ccataaccgc gatagtcgaa aataaaaatg ttgatgcacg tcttatcgtg
     6721 caatagctta atattagcga cccggtcgcc aatgttgccg gcattgccat gaaaccaaat
     6781 catggtggtg ccggctttgg gatggggaat gaaccaaccg ttcagacgaa cgttgtccac
     6841 cgtagtgaag tagacatcct gatatcctaa tcctacgtcg cgtggtgtac gcaaaatttc
     6901 tgtgctggga tgaaagatca gccggttttc caaaccgcag ccggtcaaaa agaagagcag
     6961 caccgtaaaa aaacttgccg cgccagattg tccggatgac tccgcttgaa tgctcatttg
     7021 acgccacgtc cccttggtgg tcggtcgtac aggtataaga cggaagcggt ttgcgctact
     7081 gctgaggtcg ccgcaactga catcagttca aagagttaag ccctgaccgg ttcatgatat
     7141 cgattccggt gccttgatca aagtaaaatc tagaagcgcc atgtcgatct cgcctgacga
     7201 ttggcaaagc atccaagcga taaactttgc agtagcgcgc aagtctggta tccttccgta
     7261 gatgcccgag gacgatagta taacggaccg tagcctgcag ggttattttc tccaagtggt
     7321 gcgtcagacc ttttggcaag tgggactgta tgacgcgact gtggcggagt atatcgccaa
     7381 agttctggcc gaatttgcgt tggccgataa tctctacaag attcgcgatc ggggtggccg
     7441 gaagatcgac agcgttgtcg agttgttggc taaagcaccg gttgttccag cggacgaggc
     7501 tgatatttta cgccagcgca cgttacggaa atacgtgggt gactacgcgc tgtttatgag
     7561 cggtgttttt cgtacgcaca tagacggcaa gggctcctta ggttattacc ttcaggaggg
     7621 aagccgctct tactggacgg tctccgagct cgatctcgcg ctgtacagga ccggctatta
     7681 tctctatcaa gaactttcga ccaagttcga gtactattcc ggggcgctcg atttcatgcg
     7741 caaaagctac tttgccgcgg cgccgggtga ggatccattc gcaggtttcc tgcggcaaat
     7801 aaaaggttgg atgaaaatcc atgtgagcga gaattgagcg ctaccggagc agtcatcgcc
     7861 acgctccgta cggaacctca aaagacgtga aactcctccg caccgtggtt ctttctacag
     7921 ttctggtgtt gccactgttg gccgcgtggt tcttttacaa cataaattat tcgcggcccg
     7981 ccagtggtgc gtacccgatt gaagtgagag ttgagtcggg cgattcattt gccacggtga
     8041 cgcgaaagtt gcgcgagcaa caggtgattt ccaacggttt gtttttttcc ttgtgggccc
     8101 gcttcaaggg ttcggagaaa aaagtccacc agggcctgta ccggttcgag actagcgtca
     8161 cgccgtcgga agtgttggaa cgactggtca ccggtaaggg aattttttac accgtgacga
     8221 tccctgaagg gttgacggtc aaggaaatag ccgagctgct ggacaagatg cagatcgccg
     8281 atggagagaa gtttctcgct gtcgccgccg atcccgcagt gcttggcgcg ctcggcctcg
     8341 gtgaccgagg tctcgaaggt tatctcttcc caagcactta tcactttatc ccggccacgc
     8401 cggaacggga cattattggt gccatgactg agcagttccg caagtcatct cagccattgt
     8461 tggctaaagc atcgatggca cagctgacta cgcacgaact gctgaccct
//
LOCUS       DATLCH010000072         6459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10553615, whole genome shotgun sequence.
ACCESSION   DATLCH010000072 DATLCH010000000
VERSION     DATLCH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10553615"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VMZ02_02070"
     CDS             <1..756
                     /locus_tag="VMZ02_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70743.1"
                     /translation="IGNYVFRNSVTEADILPMTLKVATQKTGLKKVAVLYGNDDIFTK
                     SGYDNFKKTLEALKIPVTTTETFAKGDVDFKPQLTKIKASNPDAVVLSALVAEGAPVM
                     VQARQLGITIPFIGGNGMNSPKIFDLAQGNSDNLWVGSPWSIENPAAENKRFIAAYQK
                     AQSALPDQFAAQAYDAMYIVAQALKKVKLTGKLEADRSALRNALPEVQINGATGAFKF
                     RQVTGKDGKPAGYDAVQSAIVMMTKGNQYVIQK"
     gene            760..1641
                     /locus_tag="VMZ02_02075"
     CDS             760..1641
                     /locus_tag="VMZ02_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUR70744.1"
                     /translation="MLAQQLVNAISLGGVYALFALGFTLIFGVLNVVNLAHGAIFMLG
                     AYSALQAVLLFKLPLTVAILAAMIVTAAAGWLLDYFIFAPLRARQAPHLAPMIATIGL
                     AISLTSTIQGMFGAENLRFPVHGLAAETLQVGGVQITALELQIIFLAFLLMTVLLWWL
                     MHTRMGCALRAVAESRKAAAILGINVEGLFRLTSMIAASLGGVAGVLIALYTNAVFPQ
                     MGQPMLHKGIAVVILGGMGDIRGALLGGFFLGCAEVFSVAYIGSTMRDAVAFGLLFTI
                     LLLRPEGLFGKAAQRRA"
     gene            1724..2584
                     /locus_tag="VMZ02_02080"
     CDS             1724..2584
                     /locus_tag="VMZ02_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUR70745.1"
                     /translation="MEWLETFWATYSTLVLSVGTNVLLALSIYLTLACGLLTVANAAF
                     MGIGAYAAALLSLHGSLPFYLSLTLGALLPAMVAAMLGPPTLRLSGVYLAMATLAFGE
                     IVRITLLNTENLTGGALGLNGIPQYTRWFDVFAIVVGALYVLVRFAGSRIGRTMIAIR
                     QDEVATELMGHNVRAYKLYAFTLGAGLAGLAGGLNAHFTFFISPNEYGFDRGVEILAM
                     GVLGGTGSPWGAVIGGILITLLPELLRSLGDYRPLLNGVILILIILYSPNGIWDLKRL
                     IRSRHPVPVS"
     gene            2596..3363
                     /locus_tag="VMZ02_02085"
     CDS             2596..3363
                     /locus_tag="VMZ02_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70746.1"
                     /translation="MSSITKAFGGLQVLQNINLAIPEQRIFGLIGPNGAGKTTLFNIT
                     TGLLAPTSGNIEFFGHSLTGLAPHQIVRLGIARTFQNIRIFKDMSVIENVMVAMVRQA
                     RYSALRVLVPPALLGAQCVERSEARSLLARVGLAAKAGILAGSLSYGEQRRLEIARAL
                     ATRPKFLLLDEPTAGMNGTEKHQLMEDILKMAESGLSLLIIEHDMRFIMGICQEIAVL
                     NFGQLIAQGTPEAIRANPEVVEAYLGNESDGVIMFAK"
     gene            3369..4082
                     /locus_tag="VMZ02_02090"
     CDS             3369..4082
                     /locus_tag="VMZ02_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70747.1"
                     /translation="MSFLLEVTNLHVHYGAIEAVKGIDLSLQTGKITTLLGSNGAGKS
                     TTLLAISGLLNPSAGTVLFDGKEICGRAPHQIVDMGIVQVPEGREILATLTVRENLLL
                     GAYRRRHDGKIHSDLERMLAIFPRLKDRLGGAAGNLSGGEQQMLAIARALMARPKLLL
                     LDEPSMGLAPLLVREIFLTLTRLRQSGLTILLVEQNARQGLKIAEHGYVMEDGVIVLS
                     GRNDDLLQNKQVIAAYLGG"
     gene            4380..4583
                     /locus_tag="VMZ02_02095"
     CDS             4380..4583
                     /locus_tag="VMZ02_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR70748.1"
                     /translation="MDEILTAGEVAELLQIHPRTVYKLVKQGSIPGRKFGGGWRFSRT
                     DILAMVSPQGQPPAPRQLHTEER"
     gene            4744..5919
                     /locus_tag="VMZ02_02100"
     CDS             4744..5919
                     /locus_tag="VMZ02_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HUR70749.1"
                     /translation="MGGALAGIKIVEAASYVTGPFASQLLADLGAEVIKVEEPARGDP
                     FRGWGERNYAATFCSLNRNKKSVTIDFRTDEGRDVMVKLLTRADVFIQNFRPGVLDRR
                     GLGYDDIKRLNAKIVYCSISGFGPKGPYRDMPGYDTIGQARSGLLSLLTDPGKPQGMG
                     ISFSDHLTGMYACYGVLGALMNRMLTGEGQHVETSLLRASVSFIAENAARYFETGHVP
                     RRKHRTTTAGVFAFEDQDGLAFVLHMSSPDKFWLGLFEVVGKPEWANDPRFNNRKVRV
                     ENYDALNEQLAPIFRAGKREDWLRRLIAKDVPAAPINSLDEVFADPQVKSYGFPIEVE
                     HPQMGKMQLIGNAVDMSRTPPSIDCPPPMLGEQTEEVLSGLGLDANTIENLRAKGAI"
     gene            5981..6442
                     /locus_tag="VMZ02_02105"
     CDS             5981..6442
                     /locus_tag="VMZ02_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HUR70750.1"
                     /translation="MRHPSLVSLSHDHHHGLALALRCRKQALGQIKPMGSAGLRERAH
                     EVLEFYRANLLAHFQAEEEVLFPLMREQVMGCAEMLDQLVRDHEQIRRAMPQLEAGTG
                     LAKLMFDLGDLLERHIRKEERELFPLFEAHIDDAKAEQVAINIKKLLNPPS"
BASE COUNT         1389 a         1673 c         1847 g         1550 t
ORIGIN      
        1 atcggcaact atgtattccg caactcggtg acagaagccg atattttgcc gatgactttg
       61 aaggtagcga cgcagaaaac tggtcttaag aaagttgccg tgctgtacgg caacgatgat
      121 atttttacca agagcggcta cgacaatttt aaaaagactc tggaagcact caagatcccg
      181 gtaacgacca ccgagacttt tgccaagggc gacgtcgatt tcaagccgca gctaaccaag
      241 atcaaagcca gcaatcccga cgccgtcgtt ctctccgctt tggtagcaga gggtgctccg
      301 gttatggtgc aagcgcgcca gttgggtatc acgatcccgt ttatcggtgg taacggcatg
      361 aactcgccga aaatcttcga tttagcccaa ggaaattctg acaatctgtg ggtcggaagt
      421 ccatggtcta tcgagaatcc ggccgcggag aataagcgct tcatcgccgc atatcaaaaa
      481 gcccaaagtg ccttgcccga tcaatttgcg gctcaggcct acgatgccat gtacatcgtc
      541 gcccaagcgc taaagaaggt gaaactcacg ggcaaactgg aagccgatcg cagtgctttg
      601 cgcaacgctt tgcccgaggt gcagatcaac ggggcgaccg gggctttcaa gttccgccaa
      661 gtaactggca aagacggcaa accggccggt tatgatgccg tgcagagcgc aatcgtgatg
      721 atgaccaaag gcaatcagta cgtcattcaa aaatagcgga tgcttgccca gcaactcgtt
      781 aatgcgattt ctctgggcgg cgtctatgcg ctgtttgctc tcggttttac cctaatcttc
      841 ggcgtcctca acgttgtcaa tctcgcccac ggcgctattt ttatgctcgg cgcgtatagc
      901 gcgctgcagg cggtgttgct tttcaagttg cctttgacgg ttgcaattct cgccgccatg
      961 atagtcacgg cggccgccgg ttggctgctc gattacttca ttttcgcgcc gctgcgggcg
     1021 cgtcaggcac cccacttggc gccgatgatc gcgactatcg gattggcaat ttcgctcacc
     1081 agcacgatcc aagggatgtt cggcgctgag aatttgcgct tcccggtgca tggcttggcc
     1141 gcggaaaccc tgcaagtggg cggcgtgcaa attaccgcgt tagaactgca aatcatcttt
     1201 ctggcatttt tgttgatgac cgtactgctg tggtggctaa tgcacacgcg catggggtgt
     1261 gcgctacgcg cggtggcgga gtcgcgcaaa gctgcagcga tcttgggcat caacgtcgag
     1321 gggctgtttc gtctaacctc gatgatcgcc gcttctttgg gcggggtggc tggtgtattg
     1381 atcgcgctct acacaaacgc ggtatttccg caaatgggcc agccgatgtt acacaagggt
     1441 attgccgtgg tcatcctcgg cggtatgggc gacatccgtg gtgctttgct gggcggtttt
     1501 ttcctcggct gcgctgaagt gttttcagtc gcgtatatcg gctcgactat gcgtgatgca
     1561 gttgcttttg gtctcttgtt cacgatttta ttacttcgcc cggagggtct cttcggcaaa
     1621 gccgcacagc ggcgtgcttg acctgaacga cccgacgtag tgcagcgttt aagtgaaagc
     1681 tcagggttcg cagctgtaga taaattattt tctcgtcacc gaaatggaat ggctcgaaac
     1741 cttctgggcg acttacagta ctctggtgct ttccgtcggg accaacgtac tgttggcgct
     1801 gtcgatttat ctgacgcttg cgtgcgggct tttgaccgtc gctaatgccg cctttatggg
     1861 gatcggcgcg tacgctgcgg cactgctcag tctgcacggc agtttgcctt tttatttgtc
     1921 gttaacactc ggcgcgctgt tgccggcaat ggtggcagcg atgcttggtc cgccaacgct
     1981 acggctatcg ggagtttacc tagccatggc gaccctcgcg ttcggcgaga tcgtccggat
     2041 aactctactc aacaccgaaa accttaccgg aggcgctctc ggactcaacg gcatcccgca
     2101 gtacactcga tggttcgacg tctttgcaat cgttgtcggt gcgctttatg tgctcgtgcg
     2161 ctttgccggt tcgcgcatag gccgtaccat gatcgctatc cgtcaagatg aagttgccac
     2221 cgaacttatg gggcacaatg tgcgggctta caagctttac gcgtttacac ttggcgcggg
     2281 actggctgga ctcgccgggg gcttgaatgc tcacttcacg tttttcatca gtcccaatga
     2341 gtacggcttt gaccgcggcg tggagatact ggcaatgggg gtattgggcg gcacgggtag
     2401 tccttggggc gcagtcatag gcggcatact gatcacactt ttgccagagt tgctgcgcag
     2461 ccttggggat taccggccgc tgctcaatgg cgtaattctg atactgatca ttctttattc
     2521 gcccaacggg atctgggatc tgaagcgctt gatacggagc aggcatcctg tgccggtaag
     2581 ctaggtatgc tcattttgtc ttcgatcacg aaagctttcg gtggtctgca ggtgctgcag
     2641 aacattaact tggcaattcc cgaacagcgt attttcggct tgattggccc caacggagcg
     2701 ggaaagacca cgctgtttaa cataaccacc ggtttactgg cgccgacaag cggcaacatc
     2761 gaattttttg gtcactcgtt gacaggattg gcgccccatc aaattgtccg tctgggcatt
     2821 gccagaacct tccaaaatat tcgcattttc aaagatatgt cggtgataga aaatgtaatg
     2881 gttgcgatgg tccggcaagc tcgatatagc gctttgcgcg tgctggtgcc gccagctttg
     2941 ttaggggctc agtgcgtaga acgatcggag gctcggagtt tattagcgag agtcggatta
     3001 gctgcgaagg cggggatcct ggccggaagc ctttcctacg gcgagcagcg acgcttggag
     3061 atcgcccggg cgctagcgac caggcccaag tttttgctgc tcgatgagcc gaccgcgggc
     3121 atgaatggca cggagaagca ccagctcatg gaagacattc tcaagatggc cgaaagcggc
     3181 ttgagtctgc ttatcatcga gcatgacatg cgcttcatta tgggtatttg tcaggaaatt
     3241 gcggtactca acttcggcca actaatcgcc caaggcacac cggaggcgat acgcgcaaac
     3301 ccagaagtcg ttgaggctta tcttggcaat gagagcgacg gcgttattat gttcgcgaaa
     3361 tgatcgttat gagcttcttg cttgaagtca caaatctgca tgttcactac ggtgcgattg
     3421 aagcggttaa gggcatcgat ttgtcgcttc agacgggaaa aatcaccact ttgcttggct
     3481 ctaacggggc cgggaagtcg acgacgttgc tggcaatttc gggattgttg aatccaagcg
     3541 ccggcactgt tctgtttgat ggcaaggaaa tttgcgggcg cgcgccgcat cagatcgtag
     3601 atatgggaat cgtgcaggtg ccggaaggac gggagattct cgctaccctg accgttcgcg
     3661 aaaatctgct tctcggcgcg tatcggcgcc gccatgatgg gaagatccac agcgacttgg
     3721 aaaggatgct ggcaatattt ccgcgcctga aagatcggct cggcggtgcc gctggcaatc
     3781 tatcaggcgg cgagcaacag atgctggcca ttgcccgtgc tctgatggca cggccaaagc
     3841 tgctgctttt ggacgaaccc tcgatgggct tagcaccgtt actggtgcgg gaaatctttc
     3901 ttaccttaac gcggcttcgg cagagcggtt tgacgatctt gctggtggag caaaatgctc
     3961 gtcagggact caagattgcg gagcatggct atgtcatgga ggatggcgtc atcgttttat
     4021 ctgggcgcaa tgacgatctg ctccaaaaca aacaagtgat tgccgcttat ctgggtggat
     4081 aattcgcatc gcgtcacacc tgcaaaccaa agcgctagga acacgatgta tgcctcgcaa
     4141 agagtcgagg gaactgccgt aagtagacat tcataaggca ggcggaaaac ttacagattt
     4201 gttcttaatt gtaccgatca tcactatttg tgcgctcctc tctcggggta aattgctgga
     4261 tttgtccgct gattacgtag ctgtcagatc ctgtcccggc acttttgccg gcctttagtg
     4321 gcacaaaatc tgccttggtt agccttgtaa ataggcccgc aatcgaagga ggagtttcta
     4381 tggacgaaat cttaaccgca ggagaagttg ccgagttatt acaaattcac ccgcggacag
     4441 tttataaact ggttaagcag ggctcgattc cagggcgaaa gttcggtggc ggatggcgtt
     4501 ttagccggac cgatattctc gcgatggttt cgccccaagg ccaaccgccg gcgccgcgtc
     4561 aactgcacac ggaagaacgt taaacttcaa caaccaactt cattaaggag ccctccgatt
     4621 cgagacgcgc cggtctcgac ggagggcttc ttatttcaga ttccagcgct cacattccaa
     4681 ctcttgcttc ccatgtgccg agcagttagg tagtttggat aaatctgttc aaaggatggc
     4741 actatgggcg gggcattggc cgggatcaaa attgtcgagg ccgcgagtta cgtaaccggg
     4801 ccgtttgcat cgcaactatt ggccgatctc ggcgcagagg tcatcaaggt cgaggaaccc
     4861 gcgcgcggtg atccatttcg cggctggggt gagcgcaact acgctgcgac tttttgcagt
     4921 ctcaaccgca ataagaagag cgtaacgata gatttccgca ctgacgaagg gcgcgatgta
     4981 atggttaaac tactgactcg cgccgatgtg tttattcaaa actttcgccc gggcgtcttg
     5041 gataggcgcg gtttgggtta cgacgatatt aaaaggctca acgcgaaaat tgtctactgt
     5101 tcgatctccg gtttcggtcc caaaggaccc taccgggaca tgccgggcta cgacaccatc
     5161 ggccaggcac gcagcggtct attgagcttg ttgacggatc ccggcaagcc gcagggaatg
     5221 ggcatttctt tttccgatca tttgaccggc atgtacgcct gctacggtgt gttgggcgcg
     5281 ctgatgaacc gcatgctgac cggcgagggt cagcacgtcg aaacttctct gttgcgcgct
     5341 tcggtgtctt ttatcgccga gaacgccgcg cgttatttcg agaccggaca tgtgccgcgg
     5401 cgcaagcacc gcacgaccac ggccggtgtg ttcgcgttcg aggaccagga cggtttggcg
     5461 ttcgtgctgc acatgtcgtc gccggataaa ttctggctcg gcctattcga ggtcgtcggc
     5521 aagcccgagt gggcgaacga tccgcgtttt aacaatcgca aagtgcgcgt ggaaaactac
     5581 gatgcgctga acgagcagct cgcaccgatc tttcgcgccg gcaagcgcga agactggctg
     5641 cgccggttga tagctaaaga cgtgccggcg gcgccgatca atagcctcga cgaagtattt
     5701 gccgacccgc aagtcaaatc ttacggcttt ccgatcgaag ttgagcatcc gcagatggga
     5761 aaaatgcagc tcatcggcaa tgccgtcgac atgagccgga cgccgccgtc catcgattgt
     5821 ccaccaccaa tgctcggcga gcagacggaa gaggtgctga gcggactggg cttggatgcg
     5881 aacacgattg aaaatttgcg cgctaagggc gcgatctgaa caatcgaagt cggcttctcc
     5941 gactccatta ctccagtact ccaattctcg aaatgttctc atgcgtcatc ccagtttagt
     6001 ttcactatcg cacgaccatc accatgggct tgcgttggcc ttgcgttgcc gcaagcaagc
     6061 gcttgggcag atcaaaccga tgggttcagc agggttgcgt gaacgcgctc acgaggtatt
     6121 ggaattttac cgcgctaatc tcctggcgca tttccaagct gaggaggaag ttttattccc
     6181 gctgatgcgg gaacaggtga tgggctgtgc cgaaatgctc gaccagctcg tgcgagatca
     6241 tgagcagatt cgccgcgcca tgccgcagct cgaagccgga actgggttgg cgaagttgat
     6301 gtttgattta ggcgatttgc ttgaacgcca tattcgcaaa gaagagcggg agttatttcc
     6361 gctctttgaa gctcacatcg atgatgccaa ggctgagcag gtggccatta acataaaaaa
     6421 actgcttaat cctccatctt agagcagcga ccgaacagc
//
LOCUS       DATLCH010000073         5636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9808929, whole genome shotgun sequence.
ACCESSION   DATLCH010000073 DATLCH010000000
VERSION     DATLCH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9808929"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VMZ02_02110"
     CDS             complement(<1..873)
                     /locus_tag="VMZ02_02110"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70751.1"
                     /translation="MKYLIGAVLLTHFCWLHLAYANPYLAKPGDAPATVRVAACAITG
                     GFIHLYAALDNGLFDKYGLKVEFVSIRGSGVALAALAADEIQFLYCAADATIPGMASG
                     SDAKLIAAPLVGLPWVMLARKDIKRVEDLKGKSIAVTRAGDLTFRLARALLKKFTITE
                     NEVKILTVGGTGQVEPFNAMRAGLAEAALVTPPLDVRGRRDGFNLIYRLNDLALPAVY
                     SSLHTNAKTLRDRPLWAQKWAAVLAESIAFVEKNPEKAKIAVAKALKLNDADALQSAY
                     DAYAKLLINRRLTVP"
     gene            1014..1991
                     /locus_tag="VMZ02_02115"
     CDS             1014..1991
                     /locus_tag="VMZ02_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70752.1"
                     /translation="MQRIVTAVLIFLCSEFALSGVSLGQNFKVGMTSKTLFYLPFYVG
                     QKRGFYSQENVKVELVLIGRTDVQLQALLSGEIDFGTLNADSVIAVGEKGARLKVIAG
                     VDNAAPYFLIGAKNLKKVEDLKGARLGVTSLRGGATSILLEYLKSKGLFFPRDFILTV
                     IAGGTVSRLTALESGSIAGAVLGFPYSDIAVDQGFTRLGDTMEVISTYQFNGLTVNPG
                     WAEKNRPGVVRFLKAHIRSLRWIHEHPDQAADLFTAEMGIKPPYARRGVEYFTKNKVF
                     PIDGSVTVEGLKANIDIQFKDGVLKEPLPTPDRYADHSYVRQAQKELGL"
     gene            2213..3229
                     /locus_tag="VMZ02_02120"
     CDS             2213..3229
                     /locus_tag="VMZ02_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70753.1"
                     /translation="MSRRSLSASLIFLAAFGLFSVELPAQNRAKVVIGHASMSSVVLT
                     LWAAQDKGFFAKNGIDAQMVFIPGSPTLIASLNTGDVHFGFTGGTATLGAAVAGLDVR
                     VIAAFADRVQSELVARPEIKTAADLKGKRIGVTSIGGTGWMSALLILEQIGINVERDK
                     MQLTAFGDQRVISNALESGTIQGAALAGVFSKALARKGYWIPGEPDKIPLTGTGLVVK
                     ADYLHNHPAIARSVLRSLVEGHAYVLNPANKSSSMEILSKRLGIKDQSAAADGYEDYV
                     GGVVRKPFVGLDGLKNIQRFMKLRNPKIAELNLEKLIDESIMREFEKSGFLEQQFGAK
                     SGIK"
     gene            3258..4349
                     /locus_tag="VMZ02_02125"
     CDS             3258..4349
                     /locus_tag="VMZ02_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70754.1"
                     /translation="MAIDGMKVIDLDSHLVGDLESWDQTIEAKYKEFLPRKLPTKDNE
                     RRKTLVGNQIMIGSELGRQKAEKKEWVTPADLTPQGRVRNMDLDGIDVAVLSPNSPAL
                     DILWFVDDPELGAAYARAQNNYMNWYASQQKGRLMWAGVIPLQDTKEAIKELHRSREL
                     GSKALNVKATPIPGKEWSDPHFDPIFDELEKTNTPIIFHDTKTGSMGHERFADNFFFS
                     HMVGRTIESMVCLMVYLCGGIMEKHPDLKIICLETGASQMPWWLSRMDEHFEKLPHLV
                     PWQKRKPTDIFNESVFVGCEPFEDGLFEWAVEYLGGDRLVLATDSPHWDSSVPGQVTG
                     PVAKSTKISPENKRKVLGENAINLLGLGL"
     gene            4550..5563
                     /locus_tag="VMZ02_02130"
     CDS             4550..5563
                     /locus_tag="VMZ02_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70755.1"
                     /translation="MSGSLFLLLPLLILLGSYERASGAAAGKKLIIAYAAMNARVCPL
                     WAPRERGFFAKYGFDAEPLFVRGAPTLVSAMASNEIEVGYTGGTAVIGAVASGSDLKV
                     LSALTNRVTYELVARPNIKTPEDLRGKKFGVQAIGGTVWMGAILALEHLGLDPDRDKI
                     AIIAAGDQSVLAQAVAQGTIDATVLDGVQARPLANKGLNILADLGKTNLPILSSSIVV
                     REPYIQKNPQLVENVLRAVIEGAAFCLSAAQKPSTLKILQKYLRISEKDAEEGYRDMV
                     MGLDRKPYANPAGATNVIRLMKRSNPKVESVKPENLIDDRIMKKLDQSGFIDEIMAKY
                     GVK"
BASE COUNT         1279 a         1511 c         1606 g         1240 t
ORIGIN      
        1 cggtaccgtc aagcgccggt tgatcaacag cttggcgtac gcgtcgtagg ccgattgcaa
       61 ggcgtcagca tcgtttagct taagcgcttt ggcaacagcg attttggctt tctcgggatt
      121 tttttcgaca aaggcaatcg actcggcgag gacagcggcc cacttctgcg cccacagtgg
      181 acggtcgcgc aaggtcttgg cgttggtgtg tagcgaactg tagacggccg gcagggcgag
      241 atcgttaagg cggtaaatca ggttgaaacc gtcgcggcgg ccgcgcacgt cgaggggcgg
      301 cgtgaccaag gccgcttcgg cgaggccggc gcgcatggcg ttaaacggct ccacctggcc
      361 ggtaccgccg acggtaagga tctttacctc gttttcggtg atggtgaatt ttttgagcag
      421 cgcccgcgcg agccgaaagg tcaaatcccc ggcacgcgtc accgcgatac tttttccttt
      481 cagatcttcg acgcgcttaa tatctttgcg cgccaacata acccagggaa gtccaacgag
      541 tggagcggca atgagcttgg catcgcttcc ggaggccatg cccgggatcg tcgcgtcggc
      601 ggcgcaatag agaaattgta tttcgtccgc cgccagagcc gccaaggcaa cgccgctgcc
      661 gcggatcgac acgaactcga ccttcagtcc gtacttgtcg aacagtccat tatcgagagc
      721 ggcgtaaaga tgaataaacc cgccggtgat cgcgcaagcg gcaacccgca cggtcgctgg
      781 cgcatctccc ggtttcgcca aatagggatt ggcatatgcg aggtgcagcc agcaaaaatg
      841 agtcaatagc actgcgccaa tcagatactt cataaatcga taatcgcttc gagacttgcg
      901 aatacatccg cttggcggtc gaagtcaagt tggatttctt ttcttgtatt cgagcccgcg
      961 gcccatgtac tatcggcgac tctatagaat cgccaacagc ggaggattgc ccgatgcaac
     1021 ggattgtcac cgcggtctta atattcttat gtagtgaatt tgcgctgagt ggcgttagtc
     1081 ttggacaaaa cttcaaagtc ggcatgactt caaagacact tttctatctg ccgttctacg
     1141 ttggtcagaa gcgtggtttt tactcccagg aaaacgtcaa agttgaactc gttttgattg
     1201 gccgcaccga tgttcagctg caagcgctgc tcagcggcga gatcgatttc ggcactctca
     1261 acgccgacag cgtcatcgcc gttggcgaga agggcgcgag acttaaagtc atcgccggcg
     1321 tcgacaacgc cgcgccctat tttcttatcg gcgccaagaa cctcaagaag gtcgaagatt
     1381 tgaaaggcgc gcggcttggc gtcacaagtt tgcgcggcgg cgcgacctcg attctgctcg
     1441 aatatctcaa gagcaaaggg ctgttctttc cgcgggattt tattttgacg gtgatcgccg
     1501 gaggtaccgt aagtcgtttg accgctctcg aaagcggctc gattgccggg gcggtattgg
     1561 gctttccgta ttcggacatc gccgttgacc aggggttcac gcgtttgggt gataccatgg
     1621 aggtgatctc cacgtaccaa ttcaacggcc tgacagtgaa tcccggctgg gccgagaaaa
     1681 atcgtcccgg cgtggtgcgg tttcttaagg cgcatatccg ctcgctgcgc tggatccacg
     1741 aacatccgga tcaagccgct gatttattca ccgccgagat gggcataaag ccgccctatg
     1801 ctcgtcgcgg tgtcgaatat ttcaccaaga acaaagtgtt tcccatcgat ggctccgtga
     1861 ccgtggaagg attgaaagcc aatatcgaca ttcagttcaa ggacggcgtc ctcaaggagc
     1921 cattaccgac gccggatcga tacgccgacc acagttatgt gcgtcaggcg cagaaagagt
     1981 tggggttgta ggtcatttta acttgaacgg caactccaac taatgacttg attgccgtac
     2041 agcctcgatt cggctcgagt acagtctgtc atttcgagct gcagcgagaa atctctccaa
     2101 agccgcacat ccgcgtaatt cgactcgact attagatttg acggcgcaag gtggttttgt
     2161 tatccgaaag gtgcgcttat agtcactggc caatcaggag ctactcatca ttatgtccag
     2221 acgaagtctc tcagcgtctc ttatctttct agcagcattt ggacttttct cggttgaact
     2281 gccggcgcaa aaccgcgcaa aggttgtcat cggccatgct tccatgagct cggtggttct
     2341 gactctatgg gcggcgcaag acaaaggctt ttttgccaag aacggtatcg acgcgcagat
     2401 ggtttttatc ccaggttcac cgaccttgat cgcctcgctc aataccggcg atgttcattt
     2461 tggcttcacc ggcggcacgg cgaccctggg agcggcggtc gctgggctcg atgtccgagt
     2521 gatcgcggcc ttcgccgacc gcgtacagag tgaacttgtg gcccggccgg agattaaaac
     2581 cgccgccgat ctcaagggta agcgcatcgg cgtcacgagc atcggcggca ccggctggat
     2641 gtccgcgctt ttgatccttg agcagatcgg catcaatgtg gagcgcgaca agatgcagct
     2701 caccgccttt ggcgatcagc gggttatcag caacgccctc gaaagcggta ccattcaggg
     2761 cgcggctttg gcgggtgttt ttagtaaagc attggccagg aaaggctact ggattcccgg
     2821 tgaaccggac aagattccgc tcacgggaac gggattggtc gtcaaggccg attatcttca
     2881 taatcacccg gccatcgcga gaagtgtcct gcgcagttta gtcgaaggcc acgcctatgt
     2941 gttgaatccg gccaataaga gcagctcgat ggagattctt tccaaacggc tcggcataaa
     3001 agatcaaagc gccgccgccg acggctatga agattacgtt ggcggtgtcg tgcgcaagcc
     3061 cttcgtcggt ctcgacgggt tgaaaaacat tcaacgtttc atgaagctgc gcaatcccaa
     3121 gatcgccgaa ctcaatctcg aaaaattgat cgacgagagt attatgcgcg agtttgagaa
     3181 aagtggtttt ctcgaacagc aattcggcgc aaagtccgga atcaaataat cgacgacaat
     3241 caaacgagga ggaagccatg gcaatcgacg gcatgaaagt aatcgatctc gacagtcatt
     3301 tagtcggcga tctggagagc tgggatcaaa ccatcgaagc gaaatacaaa gaattcctgc
     3361 cgcgcaagct gccgactaag gacaacgagc ggcgcaagac gctggtcggc aaccaaatca
     3421 tgatcggctc cgagctcggc cggcaaaaag cggaaaaaaa agaatgggta acgcctgcgg
     3481 atcttacacc acagggacgc gtccgcaaca tggacttgga tggtatcgac gtcgccgtat
     3541 tgtcgcccaa ctcgccggcg ctcgacatcc tctggttcgt cgacgacccg gaactcggcg
     3601 ctgcctacgc gcgcgcgcaa aacaattaca tgaactggta tgcctcgcag caaaagggcc
     3661 ggctcatgtg ggccggcgtg attccgttgc aagacactaa ggaagcgatt aaagaactgc
     3721 atcgctcgcg cgagctcggc agcaaagcgc tcaacgtcaa agctacgccg ataccgggga
     3781 aggagtggtc ggacccgcac ttcgatccga tcttcgacga gctggaaaag accaacacgc
     3841 caatcatttt tcatgacacc aagaccggtt ccatgggtca tgaacggttt gccgataatt
     3901 tctttttttc acacatggtc ggccgcacca tcgaaagcat ggtgtgtctg atggtttatc
     3961 tgtgcggcgg catcatggaa aaacacccgg atttgaaaat catctgcctc gagaccggtg
     4021 catcgcaaat gccctggtgg ctgtcgcgca tggatgagca cttcgaaaag ctgccccatc
     4081 tcgtgccctg gcagaagcgc aaaccgactg acattttcaa tgagagcgtc tttgtcggtt
     4141 gcgagccttt cgaagacggc ttgttcgagt gggccgtgga atatttgggc ggtgaccgcc
     4201 tggtccttgc caccgattcg ccgcattggg actcgtcggt gccaggtcaa gtgactggcc
     4261 cagtggcaaa gagcactaag atttcgcccg agaataaacg taaagtgctc ggcgagaacg
     4321 ccatcaatct cttggggttg ggtttgtaaa accggcaggc ggcgcacttg actgtcgtcc
     4381 tacgatggcg agccgattag atttctcgct aagctcgaaa tgacaaatca cgtctatccg
     4441 tcatcccgaa cgaagcgagg gatctcttcg caatgaaccg cacggttagt cggtacgggc
     4501 ttgctggttt ttactcggtt gaaattttgt gcgcgacttt ggaggcggta tgtccggatc
     4561 gctattcttg ctgctgccgt tgctcatctt gctcggctcc tatgaacgcg ccagtggcgc
     4621 ggctgctggc aaaaagttaa ttatcgcata tgcggcaatg aacgcacggg tctgcccact
     4681 gtgggcgccg cgcgaacgcg gtttcttcgc taagtatggt tttgacgccg aaccactgtt
     4741 cgtgcgcggc gcgccgacct tagtatcggc tatggcctcc aatgaaatcg aagttggcta
     4801 taccggcgga acggccgtca ttggtgcggt cgcgtcgggt tccgacttga aggtattgtc
     4861 ggccctgacc aatcgggtga cctatgaact ggtggctcgc cccaatatca aaacgcccga
     4921 agatttgcgt ggcaaaaaat tcggcgtgca ggcaatcggg ggcacggtgt ggatgggcgc
     4981 tattctggct ctagaacatt taggtctcga tcccgaccgc gataagatcg cgatcatcgc
     5041 cgctggagat cagagcgtac tcgcgcaagc ggtggcccag ggcaccatcg acgcgacggt
     5101 tctggacggc gttcaggcac ggccactcgc gaacaagggg ctaaacattc tcgccgatct
     5161 cggcaagacc aatttgccga tcctcagttc ctcgattgtc gttagagagc cttatattca
     5221 gaaaaacccg cagctggtgg aaaatgtttt gcgcgctgtc atcgaaggcg cggcattttg
     5281 cctgtcggcc gcgcagaaac cgagtacgct gaagatcctg caaaagtacc tgcgcatctc
     5341 cgaaaaagat gccgaggaag gctatcgaga catggtcatg ggacttgacc gcaagccgta
     5401 cgccaaccca gctggggcga ctaacgttat ccgcctaatg aagcgcagta atccgaaagt
     5461 tgagagcgtg aaacccgaga acttgatcga cgaccggatc atgaagaagc tcgaccagag
     5521 cggcttcatc gacgagatca tggcgaagta tggggttaag taacggccgc accgaaccgt
     5581 tcgtctcccc gggatcgatc tagttgcagt ggcagagagt tcgtcttgtc atttcg
//
LOCUS       DATLCH010000074         3267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_238978, whole genome shotgun sequence.
ACCESSION   DATLCH010000074 DATLCH010000000
VERSION     DATLCH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238978"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VMZ02_02135"
     CDS             <1..864
                     /locus_tag="VMZ02_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70756.1"
                     /translation="DAAGIEYAVLYPTVAGVGGQNFGRIEDAELELACVRAYNDWLLE
                     EWSSFSPRFVPQCLVPLSPIDSTVNEIRRCVARGHRGVIYPSIPMELRDLPHINDALY
                     DPLWAVCQELAVPICFHAGASAKIQIPAYEGYAPATATAIQAITGPASSVSVLVNLLI
                     SKILMRFPKLQIVLAGSGLGWGAYLLEYTDFQATGDQLPQNGYDLMPSELFRRQCYLV
                     GWYDRASLRVRDYIGTENILWSSQFPQATSSWPNTQAALKKSFDGVSEDDKNKILWHN
                     AAKLYKIESKR"
     gene            977..2068
                     /locus_tag="VMZ02_02140"
     CDS             977..2068
                     /locus_tag="VMZ02_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70757.1"
                     /translation="MADTPLTAEQEVNERVAYISPYEQWKKSEGLQTYRGLAIKNLYD
                     LELSPWKSSGDGLAAFLNLEGTGGFNDSYVAEIPPGKSLKAHRHLFEETIYILKGQGA
                     TSVWIDPSRKETFEWQEGSYFSIPMNAWHEHHNASGLDSARYIAMTSAPRVIDTFNNY
                     EFVFENPFVFKDRYNSEEGYFKQAAPAQQWATNFVADVRVCETYSGGFDRGGGAKSTV
                     FRMVKQTVKSHVSHWGVGAYKKAHRHGPGIHVLIIRGTGYSILWQEGKAMERVEWGPG
                     SVFVPPEMWFHQHFNGSAEPVFFLAIGWGSDKPKAGGKAYVYKSVKEGGDQIEYEDED
                     PKIHAEFESAMKKAGARCKMEYHPHCTMK"
     gene            2109..3185
                     /locus_tag="VMZ02_02145"
     CDS             2109..3185
                     /locus_tag="VMZ02_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70758.1"
                     /translation="MKKLPATLLVFLLGTIFTTSPAFGASIEALYGEINKLPAAQRQK
                     RLEDGARKEGAFKFYGISNAALLGAYTAGFMKRYPFIKAEFWRGSGNKLVFRTLTEHR
                     AGQLDTDAVLVGTENVMTLKRSGIWTRYHSPESANFGKEYTDKDGYWHADSLGVSTLG
                     YNTKLVKKEEAPRGYDDLFDAKWRAALSIDMEPERALMSWLTAWGEQKTRAFVEGLIK
                     HGATVRRGHTLQAQLLCAGEFKVAVEIYPDGLLRMKQGGCPAALVFPNPTPAHISGAI
                     GIYVNTKNPHAAALFVDFMKSAEGAKILAATGRISGRKGVKSLYDELNNLEERGVPLL
                     IVGPEQSELVAKPMERIMKDILVP"
BASE COUNT          758 a          858 c          956 g          695 t
ORIGIN      
        1 gacgcagcgg ggatcgaata cgccgtgctc tatcccactg tcgccggcgt cggcgggcaa
       61 aatttcgggc gcattgagga cgccgagttg gaacttgcgt gcgtgcgagc ctacaacgat
      121 tggctgctcg aagagtggag cagcttcagc ccacgtttcg tgccgcagtg tcttgtgccg
      181 ctttctccga tcgactcgac ggtcaatgaa atccgccgct gcgttgccag gggccaccgc
      241 ggcgtgatct atccgtcgat tccaatggag ctgcgcgatc tgccgcatat caatgacgcg
      301 ctctacgatc cgctatgggc ggtttgccag gaactggccg tgccgatctg tttccatgcc
      361 ggtgcttcgg cgaagattca aatccccgcc tacgaaggct atgcaccggc gacggcgacg
      421 gcaattcagg cgattaccgg cccagcgagc tcggtgtctg ttttggtcaa tctgctgatt
      481 tcgaaaattc tgatgcgctt tcccaagctg caaatcgtgc tcgcagggag cggtctcggc
      541 tggggtgctt atttactgga gtacaccgac tttcaagcta ccggcgacca gctgccgcaa
      601 aacggctatg atctcatgcc gtcggaactc tttaggcgac aatgctactt ggtcggttgg
      661 tacgatcgcg ccagcttgcg tgtgcgcgat tacatcggca cggagaatat tctttggtcg
      721 agccaattcc cgcaagcgac atcgagctgg ccgaataccc aagcggcgct aaagaaaagt
      781 tttgatggtg taagcgaaga cgataaaaac aaaatacttt ggcacaatgc ggccaagctt
      841 tataagatcg aaagcaagcg ttagggactg ttttttccgt tcggcgagga accggaaccg
      901 agatcgagtg aattgcgcca tcgacattct caatattcag ctctactgat tcaaatcaaa
      961 acgtaggaga caaattatgg cagacacccc attaacagca gagcaagaag ttaacgagcg
     1021 agttgcctat atctcgcctt atgagcaatg gaaaaagtcc gagggcttgc agacctatcg
     1081 gggcctggcg atcaaaaatc tttacgatct cgagttgtcg ccgtggaaat cgagcggcga
     1141 tggcttggcg gcgtttctca atctcgaggg caccggtggc ttcaacgatt cttatgtcgc
     1201 ggaaattccc cccggtaaat cactcaaggc gcatcgtcac ttattcgaag agaccatcta
     1261 cattctcaaa ggccagggcg cgacctcggt gtggatcgat cccagtcgca aagagacctt
     1321 cgagtggcag gagggcagct atttctcgat accgatgaat gcctggcatg aacatcacaa
     1381 cgcgtcgggc ttggattcgg cgcgttacat tgccatgacc agcgcgcccc gggtaatcga
     1441 tacgttcaat aactatgagt ttgtttttga aaatccattt gtcttcaagg accgttacaa
     1501 ttcggaggag ggttatttca agcaggcggc gccggcgcag cagtgggcga ccaactttgt
     1561 cgccgacgtg cgcgtgtgcg agacttactc cggcgggttc gaccgcggcg gcggcgccaa
     1621 gagcacggtg tttcgcatgg tcaagcagac ggtgaaatct catgtctcgc attggggcgt
     1681 cggtgcttac aagaaagccc accgccatgg gccaggcatt catgttttga ttatccgcgg
     1741 caccggatat tcgatcctat ggcaggaggg taaagcgatg gagcgcgtcg agtggggtcc
     1801 cggcagtgta ttcgtgccgc cggaaatgtg gtttcaccag cacttcaacg gcagcgcgga
     1861 gccggtgttt tttcttgcca tcgggtgggg cagtgacaaa cccaaagccg ggggcaaagc
     1921 ctacgtctac aagagcgtca aagaaggcgg cgatcagatc gagtacgaag acgaagatcc
     1981 caagattcac gcggaatttg aaagcgccat gaaaaaagcc ggcgcgcgct gcaagatgga
     2041 atatcacccg cattgcacga tgaagtaata ttcgccgcgg cttcaacgat ggacaatgga
     2101 ggatggcaat gaagaaattg ccggcaacac tcttggtgtt tctgctggga acaattttca
     2161 ccacatcccc tgcattcggc gcgtcaatcg aggcgctcta tggcgagatc aacaagctgc
     2221 cggccgcgca gcggcaaaag cggctggagg acggcgcgcg caaggaaggc gcgttcaaat
     2281 tctacggcat ttccaatgcc gcgctgctgg gcgcttacac tgctggcttc atgaagcgct
     2341 atccgtttat caaagcggag ttctggcgcg gcagcggcaa caaactggtg tttcgcacgt
     2401 tgacggaaca ccgtgccggt cagctcgata ccgacgcggt gctggtcggc accgaaaacg
     2461 tcatgacgct caagcgttcc ggcatttgga cgcgctatca ttcgccggag tctgcgaatt
     2521 tcggcaaaga gtatacggac aaggacggct attggcacgc cgatagtttg ggcgtatcga
     2581 ccctgggcta caacacaaag ctagtcaaga aagaggaggc accgagaggc tacgacgatt
     2641 tattcgatgc caagtggagg gccgcgctct ccatcgacat ggaacccgag cgcgccttga
     2701 tgagttggtt gaccgcctgg ggtgagcaga aaacgcgtgc gtttgtcgag ggattgatca
     2761 aacatggcgc caccgtgcgg cgcggtcaca cgttgcaggc gcagctatta tgcgccggtg
     2821 aattcaaagt cgcggtggag atatatcccg atgggttgtt gcgcatgaag caggggggat
     2881 gtcccgcggc gctggtcttt cccaatccga cgccggcgca tatcagcggc gccattggca
     2941 tttacgtcaa tacgaaaaat cctcatgccg ccgcactgtt tgtcgacttc atgaaatcag
     3001 cggaaggcgc caagattctc gccgcgaccg gcagaatctc cggacgcaaa ggcgtcaagt
     3061 ccctctacga cgagctcaac aatctggaag agcgcggcgt cccgttattg atcgtcggac
     3121 ccgaacaatc cgagttggtt gccaaaccga tggaacgaat catgaaagat attttggtgc
     3181 cttaggtacg ggagccgagg atgagccgca aacaacgcac aaactgtgat gccgtgtcga
     3241 tttgtttaat tgcttaagaa tatctgg
//
LOCUS       DATLCH010000075         6029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_239158, whole genome shotgun sequence.
ACCESSION   DATLCH010000075 DATLCH010000000
VERSION     DATLCH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239158"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VMZ02_02150"
     CDS             complement(<1..967)
                     /locus_tag="VMZ02_02150"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HUR70759.1"
                     /translation="MNTDFLQKELDDLSEQGLYRSLRCVDGEQGPTLLLNGREVINFS
                     SNNYLGLANHPLLCAAAKQAIDRYGCGSAASRLISGNMTLHEELEAKLARFKSTEAAL
                     VFNSGFQANIGILSTLTGAGDVIVSDALNHASIIDGCRLSKAKTVVYAHTDLDELEAA
                     LKQAAAARRRLIVTETIFSMDGDEAPLLEIVELAEKYHAMVMVDEAHGTGVCGSNGAG
                     VVAKLGLSSRIAVQMGTLGKALGGFGAYVAGSRALCDLLINRCRSFIFTTSLPPAVMA
                     MALAALDLVQSEPQRRAALRLQCQQLKLGLSRLGFELGRSESPILP"
     gene            complement(1165..1824)
                     /locus_tag="VMZ02_02155"
     CDS             complement(1165..1824)
                     /locus_tag="VMZ02_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HUR70760.1"
                     /translation="MADDKNKFEAITARAFLPAKRTVPSLRKAAEHCQGCDLYKDATQ
                     TVFGEGPARASVVFVGEQPGDQEDRQGHPFVGPAGRLLDRALEEAQIERDEVYVTNAV
                     KHFKWIWRGKRRLHQKPAVRQIMACRPWLEAEIGALEPKLVVCMGSTAAQSVLGRPVP
                     ILKERGKFLTAENLPLVFITIHPSAIYRQRDRSAQEDEYRRFAAEMKLVGRKLRGLKA
                     E"
     gene            1988..3349
                     /gene="radA"
                     /locus_tag="VMZ02_02160"
     CDS             1988..3349
                     /gene="radA"
                     /locus_tag="VMZ02_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HUR70761.1"
                     /translation="MAKAKSVYACQNCGYQSSKWLGKCPDCSQWNTFAEEHFEKATST
                     RSELSLGTKEEPTSIGDINTAEEGRVQSGIGEFDRVLGGGLVPGSVILIGGDPGIGKS
                     TLLLQAFAAISQKGLTCLYVSGEESQRQIKMRAERLAVDAPNLLVLSETSLERIIEHV
                     KKIKPGLLVIDSVQTIFSSAIPSAPGSIAQVREASGSIIVLAKKTGLTTFLIGHVTKD
                     GAIAGPRLLEHMVDTVLYFEGERGHSFRILRAVKNRFGSTNEIGVFEMKEVGLTEVAK
                     PSEIFLMERPLQVPGSAVVCSIEGTRPILLELQALVSRSFLAVPRRTTIGVDHNRVAL
                     LVAVLEKKMGAKLFDQDIFVNVAGGVHVDEPAVDLGVIAAIASSSREAALDPKSVFFG
                     EVGLAGEVRGISQAETRVKEAGKLGFERCILPASNVAQLKHIKNPRLAAVSSLQECWK
                     LLF"
     gene            3452..3537
                     /locus_tag="VMZ02_02165"
     tRNA            3452..3537
                     /locus_tag="VMZ02_02165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3485..3487,aa:Leu,seq:caa)
     gene            3803..4381
                     /locus_tag="VMZ02_02170"
     CDS             3803..4381
                     /locus_tag="VMZ02_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70762.1"
                     /translation="MPVRGTASHGESQDLATALRTIGQELADLLPLILKIEVKQDLFI
                     VTGKGLPEPTRAEGGARKILKIVWNSLVRHDAANDIVDWQLMSVPFARTYSQADLRRS
                     DNHRLDGRLGTGGLPDIYSLGERLRIVGRIVQAKGGDLVQLTKTLNSVAFEYRQPDGA
                     VHSEEYSAEELFRLQREYYADRESDRGFSTAS"
     gene            4885..6006
                     /locus_tag="VMZ02_02175"
     CDS             4885..6006
                     /locus_tag="VMZ02_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70763.1"
                     /translation="MAKSGYKIIDSDMHIMEPPDLWERYIDRKFKDRAPRGVVSHNVR
                     DLRMVYPDGRDWARQSARESDVASGRNFEKNQSTYHNDAERGWTSEVQLEAMDVEGID
                     IAVLYPTRGLRALVVDDMEAPFAAAMARAYNDWLYDFCKKDPARLIGAAMISPYDIND
                     AVAEARRCAEQLGFRAIFLRAVPLVDHQWEDDYYDPLWDALEALNLSVGLHESTGTGK
                     KQVGRRLEPNLMLRRVYAQPFEQMMALGNFCAGGVLARHPKLRVAFLEANCSWLPWLL
                     WRLDETYELEGDVWAPSLKMKPSEYFKRQCVVSIEPGEITATNLVDQFGSGRLVFSTD
                     YPHGDSKYPEAVNHFLKLPISDEHKRKILWDNCAAYYAM"
BASE COUNT         1340 a         1720 c         1647 g         1322 t
ORIGIN      
        1 gcggcaagat cggactctcg ctcctcccca gctcaaatcc gaggcggctt agcccgagtt
       61 tcaattgctg gcactggagc cggagcgcgg cgcgccgctg tggttcactc tgcaccagat
      121 cgagggcggc cagcgccatc gccatgactg ctggcggcag cgacgtcgtg aagatgaaac
      181 tgcggcagcg gttgatcaac agatcgcata atgcgcggct gccggcgacg taagcgccaa
      241 agccgccgag agctttgccg agggtgccca tctgcaccgc aattcgcgag gacaagccga
      301 gtttggcgac cacaccggcg ccgttagatc cgcatactcc ggttccatgg gcttcgtcga
      361 ccatgaccat ggcatggtat ttttcggcga gctcgacaat ctcgaggagc ggcgcttcgt
      421 caccgtccat gctaaaaatc gtctcggtca caatcaatcg gcgccgcgcg gcggctgcct
      481 gctttaaagc cgcttcgagc tcgtcgagat ccgtatgggc gtaaacaacg gtctttgctt
      541 tcgatagccg gcagccatcg atgatgctcg cgtgattgag cgcatcgctg acgatgacgt
      601 ccccggcgcc ggtcaaagtg gaaagaatgc cgatgttggc ctgaaatccg gagttgaaca
      661 ccaaagctgc ttcagtgctt ttgaatctgg caagcttcgc ctctaattcc tcgtgcaaag
      721 tcatattacc ggagatcaag cgcgacgcgg cggaaccgca gccgtagcgg tcgatggctt
      781 gcttagcggc ggcacacagt aacgggtgat tggccaaacc taaataattg ttggacgaga
      841 aattgatgac ttcccggccg ttgagcaaaa gagttggccc ctgctcgccg tcgacgcaac
      901 gcaatgaccg atagagtccc tgctcgctca aatcgtcgag ctctttctgc aaaaaatccg
      961 tgttcatgaa cgggttacaa tctaaccggt cgcggcgagc ttgtcgaagg gtccaattct
     1021 acatgtatta gacctctcca accccgggcg tcgcgattta ccgaaaacga atttttgctc
     1081 ggttaaaatc ggctagagct agccattagg tataaaacaa gaatcgatgt gctcgaatga
     1141 ctctggccag agcggttcga ctcgctactc ggcttttaga ccgcgcagtt tccgcccaac
     1201 caacttcatc tccgcggcaa atcttcgata ttcatcttcc tgggcggagc ggtcgcgctg
     1261 gcggtaaatc gctgaaggat gaatagtaat aaataccaga ggcagatttt cagccgtgag
     1321 aaacttgcct cgttccttga gaatcggcac cggccgtccg agaacagatt gtgccgcagt
     1381 tgaacccatg cagaccacca acttcggttc aagcgcgccg atctccgcct caagccacgg
     1441 acggcacgcc attatttggc gcaccgcggg cttttgatgc agccgccgtt taccacgcca
     1501 aatccacttg aagtgtttga ccgcattggt cacgtaaact tcgtcgcgct cgatctgcgc
     1561 ttcttcgagc gcacggtcga gcaaacgacc ggcaggtccg acgaagggat gaccctgccg
     1621 gtcttcctga tctcccggct gttcgccgac aaatacgacg ctcgcccgtg cggggccttc
     1681 gccaaaaact gtctgagtgg catctttata aaggtcgcag ccttggcaat gctcggcggc
     1741 cttgcgcagc gatggcacgg tgcgcttcgc tggtaggaaa gcccgtgccg ttattgcttc
     1801 gaatttattt ttgtcatccg ccacattaca aagttagcac ttagactgcg cagcgctaac
     1861 ccgttaattc aggttgattc acagacgacg cgcggtttca aaagctaccc cgctctgaaa
     1921 tcgttggctt gctagttggc agccgcctgt ttaaagtcgg cacaccgagc cgaattgtca
     1981 tttacccatg gccaaagcta agtcagttta tgcctgtcaa aattgcggct accagtcgtc
     2041 caagtggctc ggcaaatgtc ccgattgtag ccaatggaat acctttgccg aggagcactt
     2101 cgagaaagcc acctctaccc gtagcgagtt gagcctcggc accaaagaag agccaacctc
     2161 gatcggtgat atcaacactg cagaagaggg ccgagtgcag tcgggcattg gcgagttcga
     2221 ccgtgtgctc ggcggcggtc tggtgcccgg ctcggtaatt cttatcggtg gcgacccggg
     2281 catcggcaaa tccacactcc tgctgcaggc ctttgccgca atcagtcaga aggggctcac
     2341 ctgcctctat gtttctggcg aagaatccca gcggcaaatc aagatgcgcg ccgaacggct
     2401 cgctgtcgat gcgccgaacc tgctggtgct cagcgaaaca tcgctggaac gcattatcga
     2461 gcatgtcaag aaaatcaaac ccggcctttt ggtgatcgac tcggtccaga caatcttctc
     2521 gtcagcgatt ccctcggcac cggggagcat cgctcaggtg cgcgaagctt caggctcgat
     2581 tatcgtcctc gctaagaaaa ccggcttaac gacattttta atcggccacg tcaccaaaga
     2641 cggcgccatt gccggcccgc gtttgctcga acatatggtc gataccgtgc tctacttcga
     2701 aggtgagcgc ggccatagct ttcgaattct ccgggccgtc aaaaaccgct tcggttcgac
     2761 caacgagatc ggggtgttcg agatgaaaga agttggcctc accgaagtcg ccaaaccgtc
     2821 ggaaattttc cttatggaac ggccgctgca agtgcccggt tcggccgtgg tttgcagcat
     2881 cgaaggcact cggccgattc tgctcgagtt gcaggcgctg gtgagccggt cgtttctcgc
     2941 cgtgccgcgg cgcacgacca tcggcgttga ccacaaccgt gtagcgcttt tggtcgccgt
     3001 tttggagaaa aagatgggcg ccaaactgtt tgatcaagat atttttgtca atgtcgccgg
     3061 cggcgttcat gttgacgagc cggcggtcga tctgggcgtc attgccgcta tcgcctcgag
     3121 cagccgcgaa gccgccctcg atcctaagag cgtttttttc ggcgaggttg gcctcgccgg
     3181 agaagttcgc ggcatttccc aggcagaaac ccgagtcaaa gaagccggca agctcggctt
     3241 cgagcgttgc attctgccgg cgagtaatgt cgcgcagctg aaacatatca aaaatccccg
     3301 gctcgccgcc gtcagctcgc tgcaggagtg ctggaagctg ctcttctgaa cagcatcgca
     3361 tgcttcatca cccgagctaa aaagtccctc gcgttgctct tgaaaccgat tacactgtcc
     3421 gttatcaata tcattccgca ttactcctcg tgccggagtg gtggaatggc agacgcgccg
     3481 gactcaaaat ccggtagggg caaccctgtg cgagttcaag tctcgcctcc ggcaccaaac
     3541 tcctcgtcca gattctccga cccatacgta tgattgcgga cgcctcttag ccgccaaaga
     3601 cttgccacct gcctcgaaga cgaccgactt tggacagctg cgccgcggac aaacaacaat
     3661 tttgctcagc ttgccgtcga ttgccgcagt tttaaagaat tgaatccgat tttgccgggg
     3721 tcgctaaatg gcatgtcaat tgcctaattg cagcgcgaca agaatctgga ggtccgtcgt
     3781 gagaaatggc tgcgcaagcg aaatgcccgt aaggggaact gcgtcacacg gcgaaagcca
     3841 agatctcgcc accgctttga gaacgattgg ccaggaacta gccgaccttc tccctttgat
     3901 cctaaaaatc gaagtcaaac aagatctatt catagtcacc ggcaaaggtc tcccggaacc
     3961 aacccgagcc gaaggcggcg cacgaaaaat tctaaaaatc gtttggaact cactagttcg
     4021 ccatgatgct gcgaacgata ttgtcgattg gcaattgatg tcggtgccgt ttgcgcgcac
     4081 gtattcgcaa gctgatttac ggcgctctga caaccaccgt ttggacggga gattgggtac
     4141 cggcggcctg ccggatatct acagccttgg cgagcgcctg agaatcgtcg gcaggattgt
     4201 gcaggctaaa ggcggcgacc tcgtccaact cactaagacg ttgaacagcg tggctttcga
     4261 atatcgtcag cccgatggcg ctgttcactc ggaggaatac agcgccgagg agttgtttcg
     4321 gcttcagcga gaatattacg ctgaccgtga aagcgaccgc ggattttcga ccgcttcata
     4381 gaattatttc tccgatcagc cgctatccag cgtagtctgc gaatagacca cgaattttcg
     4441 cgcgctgctc tcaaccacct gctcagcttc gaatacagcc acgctactga aacctgcccg
     4501 tccgctgccg tgcaatcgcg tgataccgtc gatcgctccc cgcggcagca aaaaattaga
     4561 aaccaacatt gcgaccgggc aatcaccgcg acggagcgaa cggcaacaaa gcttaccaat
     4621 cgaaatcttc gcgcaggcag cagattctta tcttctccgc agttgctcag tacgccacag
     4681 cttctcgact ttggctcact gcacgcccgt tcaatccgaa tcgccgccga gaaatactgc
     4741 cgataaattc ttcgatcctt cgcgcagtgc attatcagcc ttgattgatc cttcgcatta
     4801 aatgtgattt ttgttttgct tacacataat agcgacgcgc aattgaaaca gtgcgccacg
     4861 cgaacttagc gggaggaacg actcatggcc aagagtggct acaaaataat cgatagcgac
     4921 atgcatatta tggaaccgcc ggatttatgg gagcgctata tcgaccggaa gttcaaggac
     4981 cgcgctccgc gcggggttgt cagtcacaac gtccgcgacc tacgcatggt ttatcccgat
     5041 ggtagagatt gggcgcgtca atcggcacgc gaaagcgacg ttgctagtgg ccgcaatttc
     5101 gagaagaatc aaagcactta ccacaacgac gccgagcggg gctggacgtc cgaggtccag
     5161 ctcgaagcga tggacgtcga aggcatcgat atcgcggtgt tgtatccaac gcgcggcttg
     5221 cgcgccttgg tagtcgacga tatggaggcg cccttcgccg ctgcgatggc gcgcgcctat
     5281 aatgattggt tgtacgattt ctgcaagaaa gacccggcgc gtttaatcgg tgcggcgatg
     5341 atctcgcctt atgacatcaa cgatgccgtc gctgaggcgc ggcgctgtgc cgaacagttg
     5401 ggctttcgcg cgattttttt gcgcgcggta ccactcgtcg accatcaatg ggaagacgat
     5461 tactacgatc cgctgtggga tgcccttgaa gccctgaatc tttccgtcgg cctccatgaa
     5521 tcaaccggaa ccggcaagaa gcaggtcggt cggcgcctcg agcccaatct gatgctgcgc
     5581 cgggtttacg ctcaaccgtt cgagcagatg atggctcttg gcaatttctg cgccggcggc
     5641 gtgctggcgc gccatccgaa attgcgtgtg gcatttttgg aggccaattg cagctggtta
     5701 ccctggctgc tgtggcgatt ggatgaaacc tacgagctcg aaggcgacgt ctgggcgccc
     5761 tctcttaaaa tgaaacccag cgagtacttt aagcggcaat gcgtggtatc aatcgaaccc
     5821 ggcgagatta ccgccaccaa cttggtcgac cagtttggca gcggtcggtt agtgttttcg
     5881 acggattacc cgcacggtga ttccaaatac cccgaggcgg taaatcactt tctcaagctg
     5941 ccgatctccg acgaacataa acgtaaaatt ctctgggaca attgcgcagc gtattacgca
     6001 atgtgattgg cgtgatgaat cggcgtggg
//
LOCUS       DATLCH010000076         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5458884, whole genome shotgun sequence.
ACCESSION   DATLCH010000076 DATLCH010000000
VERSION     DATLCH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5458884"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VMZ02_02180"
     CDS             <1..240
                     /locus_tag="VMZ02_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70764.1"
                     /translation="FPGLLDPKTGKTRVRSVDIYSPSYRVAREYMIRLEKEDFRDEEK
                     LRQLAQAATSAQHLCSPEQFRDRYQYLTTDLRGCN"
     gene            252..1265
                     /gene="gap"
                     /locus_tag="VMZ02_02185"
     CDS             252..1265
                     /gene="gap"
                     /locus_tag="VMZ02_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HUR70765.1"
                     /translation="MASKVAINGFGRIGRLVLRTLLERCRNELAVVAVNDMADLQTNV
                     HLFRYDTTYGTYPGKVESADGVMKIDGQSIAVLNQKEPARLPWKQLGVDIVIEATGVF
                     TDGAQVRAHLEAGAKKAIITAPATNEDITLVLGVNDGAYNPRKHHIVSNASCTTNCLA
                     PMAKVLHDVFGIERGLMTTVHSYTNDQRILDLMHKDLRRARAAAMNIVPTSTGAAKAI
                     GLVMPELKGKLHGLSLRVPTSTVSVVDLVVDLKKNASVESINAALKEKAEGQMKGILA
                     FCDEPLVSSDFRGNAASSIIDGLSTVTLEGKMAKVLSWYDNEWGYSCRVADLAKLMVA
                     KGL"
     gene            1280..1669
                     /locus_tag="VMZ02_02190"
     CDS             1280..1669
                     /locus_tag="VMZ02_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUR70766.1"
                     /translation="MNAKDIMTRDIITVSPTMSVKNLAMALIKNQISGAPVAGKNGKI
                     VGVVSEADLIAKSGKDVKSIMSKKIISVSEDTSVEEIARLMTAHTIKRLPVMKDGAIV
                     GIVSRADLVSAIAQGEHVAIHTPIYDL"
     gene            complement(1847..2290)
                     /locus_tag="VMZ02_02195"
     CDS             complement(1847..2290)
                     /locus_tag="VMZ02_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUR70767.1"
                     /translation="MKSPKKMPIVGAVMTSFPYFVQSDDPVGRLEKMMDDHGIRHLPV
                     QEKGKLVGIVSERDLHHLVPRIAPPEQKARLLARDVMVTNPYIVGFNTPLNEVVAQMG
                     VRRIGSVIVTRKGKLAGILSAMDVCRIFAEYLDNQFGTVSGNDAA"
BASE COUNT          582 a          658 c          637 g          486 t
ORIGIN      
        1 ttcccaggcc tactcgaccc caagaccggc aaaacacggg ttcggtccgt agatatttat
       61 tcgccgtctt acagagtcgc ccgcgaatac atgatccgtt tggaaaaaga agatttccga
      121 gacgaggaaa agctccggca attggcgcag gcggcgacat cggcgcagca cctctgttca
      181 ccggagcagt ttcgcgaccg ttatcaatat ttaaccacgg atttgagagg ctgtaactaa
      241 caggagagcc gatggcaagc aaagtcgcaa tcaatggatt tggtcgcatc ggccggctgg
      301 tgctgcgtac cctgctcgag cgctgcagga atgaactcgc cgtcgttgca gtcaacgaca
      361 tggcggattt gcaaactaac gtacacttgt ttcgctacga tacgacctac ggcacttacc
      421 caggcaaagt cgagagcgcc gacggtgtca tgaagatcga cggccagtcc atcgctgtgc
      481 tcaaccagaa agaacccgcc cgcctgccct ggaaacagct cggtgtcgac atagtcatcg
      541 aggcgaccgg cgtttttacc gacggcgcgc aagtacgcgc gcatctcgaa gccggcgcca
      601 aaaaagccat cattaccgcg ccggccacca acgaggatat cacgctagtg ttaggcgtga
      661 acgacggcgc ctacaatccc cgcaagcatc atatcgtctc caacgcttcg tgtacgacca
      721 attgtctcgc gcccatggca aaagtgttgc acgacgtctt cggtatcgaa cgcgggttga
      781 tgaccaccgt tcattcttat accaacgatc agagaatcct cgatctgatg cacaaagacc
      841 tgcgccgggc ccgcgcggcg gcgatgaaca tcgtgccgac gagcacggga gcggccaaag
      901 cgatcggttt ggtgatgccg gaattgaaag gcaaactgca cggcctatcg ctgcgcgtac
      961 caacgtcgac ggtttctgtg gtcgacctgg tcgtcgatct taaaaaaaac gcctcagtgg
     1021 aatcgatcaa tgcagcgctt aaagaaaagg ctgaaggcca gatgaaaggc atcctcgcgt
     1081 tttgcgacga gcctctggta tcgagcgact tccgcggtaa tgccgcgtcg tccatcatcg
     1141 acgggctttc caccgtaaca ctcgaaggaa agatggctaa ggtcttaagt tggtacgaca
     1201 acgagtgggg atacagctgt cgggttgccg atttggcgaa actaatggta gctaaaggac
     1261 tgtaacgagg agcagagcca tgaacgctaa agacataatg acccgcgata ttattaccgt
     1321 cagtccgacc atgagcgtga aaaatttagc catggcgctg atcaagaatc agatcagcgg
     1381 cgcaccggtg gccggcaaaa acggtaaaat tgtcggcgtc gtttccgaag ccgatctcat
     1441 cgccaagagc ggcaaagacg tcaaatcgat catgagtaaa aaaatcatca gcgtgagcga
     1501 agatacttcg gtggaagaaa tcgcccgctt gatgaccgcc cacacgatca aacgccttcc
     1561 ggtgatgaag gacggcgcca tcgtcgggat cgtcagccgc gccgatcttg tcagcgccat
     1621 cgctcaagga gagcatgtgg cgatccacac accgatctac gatctttgaa atcctcgccg
     1681 tagcgattaa aagactgtct ctagtccggc ggcattcgag cttcaccgga aacgggaata
     1741 aagaagcgcg tcgacttgcc agttgcggcc gttggaccaa attctccgtt ttcagacgct
     1801 tcgcctatgc aagcgcggat ctgacgatca gcgcctcaag gcgctttcat gccgcatcgt
     1861 tgccggagac agttccgaat tgattatcca agtactcggc gaagatgcgg caaacatcca
     1921 tggcggagag gattcccgcc agcttgcctt tgcgggtgac gattaccgaa ccgatccgcc
     1981 gcactcccat ttgcgcaacc acctcgttca acggtgtatt gaacccgaca atgtagggat
     2041 tcgtcaccat gacgtcgcgg gcaagcagcc gggccttttg ttccggcggt gcaatgcgcg
     2101 gcaccagatg gtgtaaatcg cgctctgata ctatgccaac caatttgccc ttttcctgaa
     2161 ccggcaggtg acggataccg tgatcgtcca tcatcttctc caaccggcca acggggtcgt
     2221 cgctctggac gaagtaggga aacgatgtca taaccgcgcc gacgatcggc atttttttgg
     2281 gtgacttcac aagcgctcct ccttaatttt gcgtagtggt tcaggtcgag ttagtcacac
     2341 gtcttgatca tgacaaaaat ttc
//
LOCUS       DATLCH010000077         4460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8317628, whole genome shotgun sequence.
ACCESSION   DATLCH010000077 DATLCH010000000
VERSION     DATLCH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8317628"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VMZ02_02200"
     CDS             <1..372
                     /locus_tag="VMZ02_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUR70768.1"
                     /translation="YNTYAGMKQLDQEFVDLARLMGANRWRLSFRVILPAVSPYIITG
                     FKTSIPFSVIGAITGEFIAATEGLGYYIRAAAGVFRTADVFVGIAVLMIIVILMNKIA
                     DLIERRVLRWQTQTESVQVQG"
     gene            383..1426
                     /locus_tag="VMZ02_02205"
     CDS             383..1426
                     /locus_tag="VMZ02_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70769.1"
                     /translation="MFKKLCALAITVAVVVAGAVSVGAQQKEEITITTAVSNLAFAAV
                     WVAEQLRYFEQEGVRAKVVVAGGGSQCQSAVVGRSAQYCASSSEGLILAYAEGAPLIA
                     VQSHNRNMTLTVGVRKAIVDKLKLTRKSPLQERMQVITHLGTIGTTGPGAVSEQIFRF
                     MANKVKGADSSKLKFAALGGNELVPAMMNNVIDGFAQSPPSVEITEAAGKGYALIPMG
                     FGEIPEFTDYPFEVLMARPDYAEANRETTTAVARAISRAGVLYHSKPNEFKAALRAHK
                     YSDKSKLEDDVFNLAYSMVAPAMPKWGNMTVEGWNKVINFAAGAGMLKDRAKIPTATE
                     GLLWTNKFVGKAP"
     gene            complement(1547..2221)
                     /locus_tag="VMZ02_02210"
     CDS             complement(1547..2221)
                     /locus_tag="VMZ02_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR70770.1"
                     /translation="MNPALYFKQLEIGPMQNYVYLIGSTATHQAAVVDAAWEIDTIIK
                     TAAQDGMEITHALVTHTHPDHVGGGFAGMAIDGVSELLEKCKAKVVVHKAEAEFLKGL
                     VASDLIKTDHGDKIDVGGIEIELMHTPGHTPGSQCFLVDGRVVSGDTMFIDACGRVDF
                     PGGNPEQMYYSLTQKLMALPEETILFPGHNYAPLKHATIGEQKKTNPYLKFASLKQFL
                     AVMGHR"
     gene            complement(2271..2711)
                     /locus_tag="VMZ02_02215"
     CDS             complement(2271..2711)
                     /locus_tag="VMZ02_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HUR70771.1"
                     /translation="MAVKEITPQQAYDTLSADNKVVYIDVRTTQEFANGHAAGAVNIP
                     VAFADPGRGMVLNPDFIRVVEKNFSKDKQIIVGCQAGPRSTRAAGMLLEAGYQDVSNL
                     LGGFGGMRDQMGNIIAPGWSASGLPMSHDNGDGVSYDWLAAKGR"
     gene            complement(2802..3572)
                     /locus_tag="VMZ02_02220"
     CDS             complement(2802..3572)
                     /locus_tag="VMZ02_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HUR70772.1"
                     /translation="MGLLDGKKAAIFGVANERSIAWAIAEAFHAEGAQLAFTFAGEML
                     EKRVRPLADGIGSKIILPCDVTKDEEVEKVFATLKQEWGGLDILIHAIAFANKEDLAN
                     PYVQTSRAGFHLAMDVSAYSLVSLTRHAAPLMEGRNGSVLTLTYMGSEKVIPNYNVMG
                     VAKAALEASVRYLAHDLGSKGIRVNAISAGPLRTLAAAGISDFKSMLHHVAERAPLKR
                     NIDAEEVGKTALYLCSDWGSAVTGEVMHVDAGYNIMGM"
     gene            complement(3622..4227)
                     /locus_tag="VMZ02_02225"
     CDS             complement(3622..4227)
                     /locus_tag="VMZ02_02225"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HUR70773.1"
                     /translation="MAVRINKVYTRTGDRGETGLVGGKRVAKDSLRVEAYGAVDELNS
                     VVGLARVFNEENRESGEGHRFLDEVLCQVQDELFDIGSELATPPEFFQAGMYRVGADE
                     IKRLEKWIDQCQKDLEPLQSFILPGGGRIGAYLHQCRTVCRRVERDVLRLSREELIND
                     DVIKYLNRLSDLFFVLARWISDQMGEPEYLWQRGLSGQTKK"
BASE COUNT          993 a         1296 c         1170 g         1001 t
ORIGIN      
        1 tataacacct atgccggcat gaagcagctc gatcaggaat tcgtcgattt agcgcggcta
       61 atgggcgcga atcgctggcg tttgtccttc cgcgttattt tgccggctgt atcgccttat
      121 attatcaccg gattcaaaac cagcatccca ttctcagtca tcggcgccat caccggtgaa
      181 ttcatcgccg caaccgaagg actgggctat tacatccgcg ctgccgccgg cgtgtttcga
      241 accgccgatg tatttgtcgg cattgccgtg ctgatgataa tcgtgatcct aatgaacaag
      301 attgccgatc tgatcgaacg gcgcgtgttg cgctggcaga ctcagaccga aagcgtccaa
      361 gtccaaggat aaggaggttt ccatgtttaa aaaactttgc gcgctcgcga tcaccgtcgc
      421 cgtagtcgtc gctggcgccg tcagcgtcgg cgctcagcaa aaagaagaaa tcaccatcac
      481 cacggccgtg tcgaatctag cgttcgcggc cgtctgggtg gctgaacagt tgagatattt
      541 cgagcaggaa ggtgtgcgcg ccaaagtcgt cgtcgccggc ggcggctcgc agtgtcagag
      601 cgcagtcgtc ggccgctcgg cacagtactg cgcgtcatcg tcggaaggct tgatcctcgc
      661 ctatgccgag ggcgcgccgc tgatcgcggt gcagtcgcat aaccgtaaca tgacgctcac
      721 ggtcggcgtc cgcaaagcaa tcgtcgataa attaaagctc acgcgcaaaa gtccgctgca
      781 agaacgcatg caagtgatta ctcaccttgg caccatcggc accaccggcc ccggtgcggt
      841 gtcggagcag atttttcgct tcatggccaa caaagtcaaa ggtgccgact cgagcaaatt
      901 aaagttcgcc gctctgggcg gtaacgagct ggtaccggca atgatgaaca acgttatcga
      961 tgggttcgcc caatcgccgc cgtcggtgga aatcaccgaa gccgccggca aaggctacgc
     1021 gctgattccg atgggctttg gcgagatccc ggaatttacc gattaccctt tcgaagtctt
     1081 aatggctcgc cccgattacg ccgaggccaa ccgtgagacc accaccgcgg tggcacgcgc
     1141 gatctcccgc gccggagtgc tttatcacag caagcccaat gagttcaaag cggcgttgcg
     1201 cgcccataaa tattctgaca aatcgaaatt ggaagacgac gttttcaacc tcgcctacag
     1261 catggtcgcg cccgccatgc ccaaatgggg caacatgact gtggagggct ggaacaaagt
     1321 catcaacttc gctgccggtg ccggcatgtt gaaagaccgc gccaagatcc cgacggccac
     1381 tgaagggcta ttgtggacta ataagtttgt cggtaaggca ccgtgaaccc ggaggaatta
     1441 aaaaattaca gattccaagt tccggcttgg gaatctgcaa ccgatcggac ggcggctatt
     1501 ttgcaatctg gaatttgtaa tctcgaatcc gctgatcgca cagcgatcag cgatgcccca
     1561 taaccgccag gaactgtttc agtgaagcaa acttcaaata cggattggtc ttcttctgtt
     1621 cgccaatggt ggcgtgtttg agcggcgcgt aattgtgacc ggggaataga atcgtttcct
     1681 ccggcaatgc catcagcttt tgtgtaaggc tgtagtacat ctgctcggga ttgccgccgg
     1741 ggaagtcgac tcggccacag gcatcgatga acatggtatc gcccgacacc acgcggccgt
     1801 cgaccaggaa acactgcgag cccggtgtgt ggccgggggt gtgcatgagc tcgatctcga
     1861 tgcctccgac gtcgatctta tcgccgtgat cggttttaat caggtccgat gccaccaagc
     1921 ctttaagaaa ctccgcctcg gccttgtgca ccaccacctt ggctttgcac ttttctaaca
     1981 gctcgctcac gccgtcgatt gccatcccgg caaacccgcc gccgacatga tccgggtgcg
     2041 tgtgggtaac caacgcgtga gtaatttcca tgccgtcctg cgccgctgtt ttgataattg
     2101 tgtcgatctc ccaggcggcg tcgacaactg ccgcctggtg cgtcgccgtc gaaccgatca
     2161 gatagacata gttctgcatc gggccgatct cgagctgttt aaaataaagc gcaggattca
     2221 ttgtacgatc gagttttttg gaatttggaa tctgtaattt gaaatttgct tcagcgccct
     2281 ttcgctgcca gccaatcgta actcacaccg tcgccgttat cgtggctcat cggcaaaccg
     2341 gaagcggacc agcctggcgc aatgatattt cccatctgat cgcgcatacc gccgaatccg
     2401 ccgagtaaat tggagacgtc ctgataacct gcctccaata acattccggc cgccctggta
     2461 gagcgcggac ccgcctggca tccgacaatg atttgtttat ctttagaaaa gtttttttcg
     2521 acgactctga taaagtcagg attcaacacc ataccgcgcc ccggatcggc aaacgccacg
     2581 ggaatattta ccgcaccggc agcgtgacca ttggcgaact cttgtgtcgt gcgcacgtcg
     2641 atgtagacga ccttattatc agcgctcaga gtgtcgtagg cttgttgcgg cgtgatctct
     2701 ttgacggcca tagaacttat ttatcagttc tcagcgggcc agttaataga agagatagat
     2761 ccccaactca acaactgacg acctaccctg atcagtatct cctacatgcc cattatattg
     2821 tagccggcgt cgacatgcat gacttcgccg gtcacagcgc tgccccagtc gctgcacaga
     2881 taaagcgcgg tcttgccgac ttcttcggca tcgatatttc ttttcaacgg agcgcgctca
     2941 gcgacatggt gcagcatgga tttgaaatcc gagatacccg ctgccgccaa tgtccgtaac
     3001 ggtccggcgg aaatcgcgtt gacgcggatg cctttggatc caaggtcgtg agccagataa
     3061 cgcacactcg cttccaacgc ggccttcgcc acgcccatga cgttgtagtt agggatgacc
     3121 ttctccgaac ccatgtaagt cagcgtcagt accgagccat tgcgtccttc catgagcggt
     3181 gcggcatggc gcgtcaggga caccaatgag taagcgctta catccatggc aagatgaaaa
     3241 ccggcgcggc tggtctgcac atagggattc gccagatctt ctttgttggc gaaagcgatg
     3301 gcatgaatca aaatgtcgag gccgccccat tcttgcttca gcgtcgcgaa aactttctcg
     3361 acttcttcgt ccttggtgac gtcgcagggc aggataatct tggaaccgat tccatcggcc
     3421 agtggccgca cgcgtttttc gagcatctcg ccggcaaatg tgaatgccaa ctgggcaccc
     3481 tcggcatgaa atgcctccgc gatcgcccag gcgatgctcc gctcgttggc aacgccaaaa
     3541 atcgccgctt tttttccgtc aagtagtccc atcgctattc accctccccg gcacttcagc
     3601 gtccggagta ttgcggcgtg gttatttctt ggtttgaccg ctcagtccgc gctgccaaag
     3661 atactccggc tcgcccattt gatcgctgat ccagcgtgcc aaaacaaaaa ataaatcgct
     3721 cagccggttc aaatacttga tcacgtcgtc gttgatcaac tcctcgcgcg acaagcgcaa
     3781 gacatcccgt tcgacgcgcc ggcacaccgt gcggcattgg tgtaagtagg cgccgatgcg
     3841 gccgccgccg ggcaaaatga aggactgcaa gggctcgaga tccttctgac actggtcgat
     3901 ccacttttcc agccgtttga tttcatcggc gccgacgcga tacatgcccg cttggaaaaa
     3961 ctccggcggc gtcgccaatt cgctgccgat gtcgaacagc tcgtcttgga cctggcacag
     4021 tacttcgtcg agaaagcgat gcccttcgcc ggattcgcgg ttttcttcat tgaacacccg
     4081 cgccaatccg acaaccgaat tgagctcgtc gacggcgccg taagcttcga ctctgagcga
     4141 gtccttggca acgcgcttgc caccgaccag accggtctcg ccgcggtcac ccgtgcgcgt
     4201 gtaaacttta ttgatgcgaa cagccataat tgaaatctac ggacaatcga taattgagaa
     4261 tgctaaaccg ggagaacggc tcacgacttt gttgatctca atctccataa tcaaattttg
     4321 atttgcaccg accgcttgcg ccaaatcaaa taagccggga tgcccaggct gacgatgaac
     4381 agtccgatca acgtctcttt gggtttttcc gtcaccgagc cccagacgat ccacagatga
     4441 accagcacga aaagtatcgg
//
LOCUS       DATLCH010000078         5648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9813676, whole genome shotgun sequence.
ACCESSION   DATLCH010000078 DATLCH010000000
VERSION     DATLCH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9813676"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..584)
                     /locus_tag="VMZ02_02230"
                     /pseudo
     CDS             complement(228..584)
                     /locus_tag="VMZ02_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(827..1243)
                     /locus_tag="VMZ02_02235"
     CDS             complement(827..1243)
                     /locus_tag="VMZ02_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUR70774.1"
                     /translation="MQVKYFEDLEIWKDARVLTRAIYQLTSDSRFSKDFGLRDQIRRA
                     AVSIMSNIAEGFERGGNQEFIQFLYVAKASCAEVRSQLYVAMDQSYLAQKDGEELAMS
                     FKRLSIMISNLMDYLKRSGMKGSKYATASRSKEAVS"
     gene            complement(1316..1924)
                     /gene="lexA"
                     /locus_tag="VMZ02_02240"
     CDS             complement(1316..1924)
                     /gene="lexA"
                     /locus_tag="VMZ02_02240"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HUR70775.1"
                     /translation="MLTPLTDRQKAIYDFLLKSIREKGFAPSIPEIGRQFKIASTNGV
                     SDHLKALEKKGYIRRVGKRAIEVVNVLGKSVLNVSREVPILGRVPAGKPFLSEENTEG
                     VLSIPSDLGSGKQFALLVQGDSMIGAGILDGDRVIVKQQTTAENGEIVCALIDGEATL
                     KRFYKKDGVVTLKAENDKYAPIVVAEGEFRIAGKIVGLLRKF"
     gene            complement(2108..3700)
                     /locus_tag="VMZ02_02245"
     CDS             complement(2108..3700)
                     /locus_tag="VMZ02_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR70776.1"
                     /translation="MVHIRYSRWSSSGGESLDSESVFDQLNENMNDTGDLQQAMRRLM
                     QKGLKQGEKQTKGIEDLLSQVAREMRKLYDEYKLQSAMDEVQEKLDSVVDQEQQTLEQ
                     MDQSGQDVQENKQFLNDLPGKTSDAIDKLTSYRFENSNAEKDFEQLVMQLEQIRKLEN
                     WLRREGSLFRGQTPMDFQQSQEMMERMEDLRKLESQLSSMQLKDVDKELLEKLLGGDP
                     KQDFEGIMRMQSLLEEGGYVLESGDRFELTPKGVRRIGQLALRDIYRQLRRDGMGRHS
                     TSKRGSQEILLETNKSYVQGDPLHINMIQTLKNALVRGGGVPVRIAPTDFSVYETELN
                     TRAATVLLLDMSWSMSWEGRFAAAKKVALAMESLVRALYPRDYFGIVGFFTRAVELKA
                     KDLPQATWNMGDPFTNLQDGLHLASTMLDRKPSANQQIIVITDGQPTAYCRQGRLYCE
                     WPLSFGGISQRAAEETLKEAERITRKGITINTFMLDDSPVLRGFVDDLTRINKGRAFY
                     SRPDRLGEYMLVDYLSHQHKKV"
     gene            complement(3734..5131)
                     /locus_tag="VMZ02_02250"
     CDS             complement(3734..5131)
                     /locus_tag="VMZ02_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70777.1"
                     /translation="MSEAKTLGELKKSGVAVLSVRDEMRKNLVRHLESGERILPGIVG
                     YDETVIPEIENAILSGHHMVFLGERGQGKSRIIRGLLSLLDERIPIVAGCEINDSPMA
                     PICSGCRKKAAELGDALPIRWVGHDQRYGEKLATPDVSIADLVGEIDPIKVAEGRYLA
                     DEETIHYGLVPRTNRGIFAINELPDLTEKVQVGLFNLMEEKDVQIKGYKIRLPLDVVI
                     VASANPEDYTSRGRIITPLKDRFDVQIRTHYPKNVEDEIHIMEQEALWTDSRSRKVEV
                     PGFMKEILGNLTFEARKSNEINQSSGVSVRVTINNFESLISNAEKRSLRCKENDIVPR
                     VSDLHALMASTAGKIEMEYVGEEKKEDELVEKLINRSIVKVFDQHFSLNSLQKVIEYF
                     NYGWGVEVSDQMAADDYLEGIKEIPGLKEAIKTLGIQESPALMASATEFVLEGLHLHQ
                     KLNKEMEGGRVTYGK"
     gene            complement(5217..>5648)
                     /locus_tag="VMZ02_02255"
     CDS             complement(5217..>5648)
                     /locus_tag="VMZ02_02255"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HUR70778.1"
                     /translation="GCIFCTKYQQSDLKAGLVLTHSRHTVVMMNKYPYNNGHLLVAPK
                     HHESSLANLTPEEYADLSEVLRRAVEIIKEVYNPGGVNVGMNLGKCAGAGIDDHLHWH
                     IVPRWDGDTNFMPVVSETRVMPQHLATSYDRLVGHFQKLIV"
BASE COUNT         1175 a         1579 c         1443 g         1451 t
ORIGIN      
        1 ttacacccaa ccgccatggt cagaacacaa aagcataaag ttgggagaaa acagcgcatc
       61 atatgcctaa ccattgagta tactgaccag tatattacgg gatccgaaaa tcacaggttt
      121 ccgaccttta tcttttcaat caacaacttg tcaattagtt tccgtccgcg gtttgtccaa
      181 tattcctggt cagtataata ccaatatacc tgacagactg ggctcgctca caatccgtct
      241 gccggacttc ggactccttt tccaccccga ttcaatacat gtgtatagat catcgtggtg
      301 ctcacatcgt tgtggcccaa gagttcctga accgtacgaa tgtcatatcc attttcgagt
      361 aagtgcgtcg caaacgaatg acgcaacgtg tgcgggccgg ctggcttgaa aacgccggct
      421 ttgagccgcg cctctttgaa agctctctgc aataccgact cgtgaagatg atgccagcgt
      481 ttttctccgc tgactctgtc gacatagtga ctggttgccg gaaataccca ctgccagccc
      541 cactctttac cggcgttcgg atacttgcga tcgagagcgt tcgggcgaaa ttcccttcgc
      601 aacgagtgga taggcttccg ccgagacggc atcaaaccgt tcaagtagtt caaattgttc
      661 aagtcgttca aaccgttgaa gagagtaacc gttccagctg ttccaatcgt tccaactggt
      721 taagacattt cggttcggcg cggtgcgctc gaacagctgc actttggccc acgcatgcca
      781 atgaagttca agccgttaaa atcgttcaaa tagtttcgga tcggagttaa gagacggctt
      841 ctttggatcg actggccgtc gcatacttcg atcccttcat cccgctgcgc tttagatagt
      901 ccatcagatt gctgatcatg atcgacaggc gcttgaagga cattgccagt tcttcgccgt
      961 ctttttgcgc gaggtaagat tgatccatcg ccacgtagag ctgtgagcgc acttccgcgc
     1021 acgatgcttt cgcgacatag agaaattgaa taaattcttg gtttccaccg cgttcgaatc
     1081 cttcggcgat attcgacatg attgatacgg ccgcgcgacg aatttgatcc cgcagtccaa
     1141 agtcttttga gaatctcgaa tcgctcgtca gttggtagat ggctcgggtt aagacccgag
     1201 cgtccttcca tatctccaag tcttcaaaat atttcacctg catatgcttt cctcctaaac
     1261 ggcttgaacg ttttgaacgg agcgcagcgg ttgaacgatt tgaacctcca aacgattaga
     1321 attttctcag cagcccgaca atttttccgg caatgcggaa ttcgccttcg gcgacgacga
     1381 tgggcgcgta tttgtcattt tccgctttga gcgtgaccac gccgtctttt ttgtaaaacc
     1441 ttttgagcgt cgcttcgccg tcgattagcg cgcagacgat ctcgccgttc tccgcggtgg
     1501 tctgctgctt gacgatcacc cggtcgccgt caagaatgcc ggcgccgatc atgctgtcgc
     1561 cttggactaa cagggcaaac tgtttgccgc tgcctaaatc gctgggaata gataaaaccc
     1621 cttccgtatt ttcctcgctc agaaacggtt tgccggcagg gacgcggccg agaatcggca
     1681 cttcccggct cacattgaga actgacttgc cgagaacatt gacgacttcg atggcgcgct
     1741 tgccgacccg gcggatataa cccttctttt ccagcgcctt cagatgatcg gaaacgccgt
     1801 tggtggaggc gatcttgaac tggcggccga tctcagggat cgacggcgca aagccctttt
     1861 cgcgaatgct cttcaaaaga aaatcgtaaa tagctttttg ccggtccgtc agtggcgtaa
     1921 gcataaagct tgtatactga aaatagtttc agtagtcaat ggcggaacaa atccatatag
     1981 gttcaagtcg ttcaaaacgt tcaaatggtt caagtcgtta aactcgaccc ggctcaaacc
     2041 gtctctaaaa cgatttgaac ggcttgaagg ttttgaacta tttgaacggt ctaacgcgcg
     2101 cggcgcgtta aacttttttg tgttgatgcg acagatagtc gacgagcatg tactcgccga
     2161 ggcggtcggg gcggctgtag aaggcgcggc ctttgttgat gcgtgtaaga tcgtcgacga
     2221 agccgcgcag caccgggctg tcgtcgagca tgaaagtgtt gatagtgatg cctttgcggg
     2281 tgatgcgttc ggcttctttt aaagtttctt cggcggcgcg ctggctgata ccgccgaaac
     2341 tgagcggcca ttcacaataa agccgtccct gccggcaata agccgtgggc tggccgtcgg
     2401 tgataacgat gatctgttgg ttggcgctcg gtttgcgatc gagcatcgtc gacgccaaat
     2461 gcaagccatc ctgcaagttg gtaaacggat cgcccatgtt ccaggttgcc tgcggcaaat
     2521 ctttggcttt gagctcgacc gcgcgggtaa agaaaccgac gatgccgaaa tagtcgcgcg
     2581 ggtataacgc ccgcaccaga ctctccatcg ccagagcgac ttttttcgcc gcggcgaagc
     2641 gcccttccca gctcatcgac caactcatgt cgagcaagag cacggtggcg gcgcgggtat
     2701 tgagctcggt ttcgtaaacc gaaaaatccg tgggagcgat tcgcaccggc acaccgccgc
     2761 cgcgcaccaa tgcatttttc aaggtctgga tcatgttgat atgcagtgga tcgccctgca
     2821 cataggactt gttggtctcc agaaggattt cctggctgcc gcgtttgctc gtcgagtggc
     2881 ggcccatgcc gtcccggcgc agctgtcgat aaatatcgcg caacgcgagt tggccgatgc
     2941 gacgcacgcc tttgggcgtc aattcgaatc gatcgccgct ctccagcaca tagccgccct
     3001 cttcgaggag cgactgcatg cgcatgatgc cttcgaaatc ttgcttggga tcgccgccta
     3061 aaagcttttc caagagctcc ttgtccacat ccttcaactg catgctcgac agctgagact
     3121 cgagcttacg caggtcttcc atgcgctcca tcatctcctg cgactgctgg aagtccatcg
     3181 gcgtctggcc gcggaacaag ctgccttcgc gacgcagcca attttcgagc ttacggatct
     3241 gttcgagctg catgaccagc tgctcgaaat ctttttcggc attggagttt tcaaaacggt
     3301 acgacgtgag cttatcgatg gcatcgctgg tcttgcccgg cagatcgttc aagaactgtt
     3361 tattctcctg gacatcctgg cctgattggt ccatctgttc gagggtttgc tgttcctgat
     3421 cgaccaccga atcgagtttc tcctgcacct cgtccatcgc cgattgcagt ttgtactcgt
     3481 cgtagagctt gcgcatctca cgcgccacct gcgatagtag atcttcgatg cccttggttt
     3541 gcttctcacc ctgcttaagg cccttctgca tcaaccggcg catggcctgc tgcaaatcgc
     3601 ccgtgtcgtt catgttctca ttcaattgat cgaacaccga ctcggaatcg agggattctc
     3661 cgccgctgga actccagcgg ctatagcgaa tatgcaccat gggtgtcttc agtcggccgc
     3721 gacccttctg cggctacttg ccgtaggtca cgcggccgcc ttccatttcc ttattgagtt
     3781 tttgatgcag atgcagtcct tcaaggacga actccgtcgc cgaagccatc agcgccggcg
     3841 actcttggat gcccaaggtt ttgatcgctt ctttaagtcc gggtatctct ttaatacctt
     3901 cgagataatc gtccgccgcc atctggtcgg agacttcgac accccaaccg tagttaaaat
     3961 attcgatcac tttctgcaaa gagttgaggc tgaaatgctg atcgaagact ttaacgatgg
     4021 agcgattgat caatttctcg accaactcgt cttccttctt ctcttcgccg acatactcca
     4081 tttcgatctt gcccgccgtc gaggccatca gtgcgtgcag gtcgctcacg cgcggcacga
     4141 tgtcgttttc cttgcagcgg agcgagcgct tctcggcatt gctgatcagg ctctcaaagt
     4201 tgttgatcgt gacgcgcacg ctgacgcccg aggattgatt gatctcgttg gacttgcgcg
     4261 cctcgaatgt caggttgccg aggatttcct tcatgaaccc cggcacttcc accttgcggc
     4321 ttcggctatc cgtccacagc gcctcttgtt ccatgatgtg gatttcatct tcgacatttt
     4381 tcggatagtg agtgcgaatc tgcacgtcga aacggtcttt gagcggtgta atgatgcggc
     4441 cgcggctcgt gtagtcttcc ggattggcgc tcgcgacgat cacgacgtcg agaggcaaac
     4501 ggattttgta gcctttgatt tggacgtctt tttcttccat caagttgaat aatccgactt
     4561 gaactttctc ggttaaatcc ggcagctcat tgatcgcgaa aatgccccgg ttggtgcgcg
     4621 gcactagacc ataatggatg gtttcttcgt cggccaggta gcggccttcg gcgactttga
     4681 tcggatcgat ctcgccgacc agatcggcga ttgacacatc gggcgtggcg agtttctcgc
     4741 cgtaacgttg gtcatggccg acccagcgga tcggcaaggc atcgcccaac tcggccgcct
     4801 ttttgcggca cccgctgcaa atgggcgcca ttggactgtc gttgatctca cagccggcga
     4861 caatgggaat ccgctcgtcg agcaagctca gcaaaccgcg gatgatgcgc gacttacctt
     4921 gtccgcgttc gcccaaaaaa accatgtggt ggccggagag aatcgcgttt tcgatttccg
     4981 gtataaccgt ttcgtcatat ccgacgattc ccggtagaat gcgttcaccg ctttccagat
     5041 ggcgcaccag atttttgcgc atctcatctc gcacggacag caccgcaacc ccgctttttt
     5101 tcagttcccc taatgttttt gcctcactca cttgacttgg cctcccctcg gaagagttgt
     5161 tgttgacgtt cggttttcgc ttggcaatac caagctacca cgttgccctg tcacacttaa
     5221 actatcagtt tttgaaaatg cccaacgagc cggtcgtagc tggtggccag gtgctgcggc
     5281 atcacccgag tctcactcac caccggcatg aagttggtat caccgtccca gcgcggcaca
     5341 atatgccaat gcaggtggtc gtcgattcct gcgcccgcgc atttgccgag attcattccg
     5401 acgttgacgc cgccgggatt atagacttct ttgatgattt cgacggcgcg gcgcaacacc
     5461 tcgctcaaat cggcgtattc ttccggagtt agattggcca gactactctc atgatgcttg
     5521 ggggcgacca gcaggtggcc attattgtag ggatacttgt tcatcatcac gaccgtgtgc
     5581 cggctgtgtg tcaggaccaa gcccgctttc aagtcactct gctgatactt ggtgcaaaag
     5641 atgcagcc
//
LOCUS       DATLCH010000079         9141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2490144, whole genome shotgun sequence.
ACCESSION   DATLCH010000079 DATLCH010000000
VERSION     DATLCH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490144"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="lptD"
                     /locus_tag="VMZ02_02260"
     CDS             complement(<1..1324)
                     /gene="lptD"
                     /locus_tag="VMZ02_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HUR70779.1"
                     /translation="MIFQLFYIMTALLVVAPWTSYSGAAQPNNPAAPINLQADKLSST
                     DSGTQIEATGNVEVKREGTTLKADELRMNRATQEVEAKGRVTLDDPEWKVKSAESLRM
                     NMENETGELHNADLFLEQGHISVSGRRFEKFGGQSYHIDDGFFTTCLCESGAPSWKFF
                     AEQMDLALEGTGTIKNGVFYIMDVPVLYIPYGYFPLRTERQTGLLFPSIGTSSKDGFR
                     FLQPFFWAISKSSDATLSFDLETRTRYGVIGEYRTVFDRNSDLKFESSYFNEGWRTTR
                     SVADSTIADPKIPLDRWNVFGSHRYTTASDWLTYSDTAVYSDDLFTRELIRRFDLPFG
                     DEGIIRRSRYSESRMGLFKSWSDTFLKGEFNFYQDFIQPDKSTFLRTPQVAFWGRRFF
                     SGIPLELRWSADGTNYLRRDRGDGLRFDLRPEAVLPFKIASVVNGSLSV"
     gene            complement(1355..2632)
                     /locus_tag="VMZ02_02265"
     CDS             complement(1355..2632)
                     /locus_tag="VMZ02_02265"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HUR70780.1"
                     /translation="MASYSETLDRIYNLRGGIIDLRLDRVAAALKTFGHPERNFPSIH
                     VAGTNGKGSTSAMLQRILCVAGYRTGLYTSPHLVSFTERIKIGDEEITQDEVVAIADE
                     IWQRTASAAIALTYFEVVTVMGFVYFARRSIDIAVVEVGLGGRYDATNVITPLVSLIT
                     TISKDHEAFLGADLLSIAGEKAGIIKPGIPVVCGALTAEVKEVFRRRAKEQESKVYFF
                     GADFNFSLKPEALFDYTGVKQHYSGLSLRLRGLHQRYNACVALAGLEAVDSYFPVAEN
                     AIRAGLATVQWPGRLEVMLERPTVVIDGAHNGEGILALVEALESYRGRKIKLLFAAMS
                     DKDWRLMLTRLIGVVSEAMFTKVAMERSTDPAELARHCAGRIAVRWNPDSKAALRILI
                     DESGPDDVIVVAGSLYLLGEIRPMVQEIAAPKP"
     gene            complement(2681..3493)
                     /gene="trpA"
                     /locus_tag="VMZ02_02270"
     CDS             complement(2681..3493)
                     /gene="trpA"
                     /locus_tag="VMZ02_02270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HUR70781.1"
                     /translation="MGRIQKTFAALKQRGEAALIPFITAGDPDLATTLKIMRALEEGG
                     ADAIELGIPFSDPSADGPTIQRSSERALKNTISLRAIFRLVREFRRSSELPVILFGYY
                     NPFFRFGLDNFATQAALAGADGVLCVDLPPEESGELKTCTDRCDLDVIFLLSPTSGAD
                     RRRLVARQGRGFVYYVSVTGVTGARQSFDGDLRRQVAQVSKTTSLPVGVGFGISTPKQ
                     AAWIAEFADAAVVGSALVQRIEQGKSKSAKVRQAGEFVAQLRRAMKRARKKT"
     gene            complement(3572..4768)
                     /gene="trpB"
                     /locus_tag="VMZ02_02275"
     CDS             complement(3572..4768)
                     /gene="trpB"
                     /locus_tag="VMZ02_02275"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HUR70782.1"
                     /translation="MLPKVPDRRGHFGQYGGRYVAETLMPALTELERSYAKVRRDGGF
                     KRELAYYLKEYVGRETPLYFAERLTRSLGGATVYLKREDLCHTGAHKINNTMGQILLA
                     RHMGKRRIIAETGAGQHGVATATVAARFGMECEVFMGEEDVQRQSLNVFRMKLLGAKV
                     RVVKSGSRTLKDAMNEALRDWVTNVRTTYYLIGSVAGPHPYPMMVRDFQSVIGREARR
                     QILQKEGRLPDHLLACVGGGSNAMGLFYPFVKDRLVKMLGIEAAGHGLGSGKHSATIQ
                     RGAVGVLHGSKSYLLQDGKGQVKETHSVAAGLDYPGVGPELSYLRDHGRVQFDSATDD
                     EAIASIRFLAQVEGIIPALESAHAIAVVRRLAPKLKKRQIIIVNLSGRGDKDMITVGD
                     HLGAQL"
     gene            complement(4749..5378)
                     /locus_tag="VMZ02_02280"
     CDS             complement(4749..5378)
                     /locus_tag="VMZ02_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HUR70783.1"
                     /translation="MPVKIKVCGITNLDDAEKSLEFGADLLGFNFYPPSPRCITPDLA
                     GEIIARLPRDAFNVALLVNETKARVSEVLAAARSRNGHDGYRALQFHGEENGAYCQGW
                     DVKVIKAFRIKEQAALDALKGFPADYYLVDSFSSGYGGSGQMFPWEWLEGIDARKILL
                     SGGLRVDNVAKAIRTVQPFGVDVCSGVESRPGVKDHGKLKEFIDAAKGA"
     gene            complement(5392..6171)
                     /gene="trpC"
                     /locus_tag="VMZ02_02285"
     CDS             complement(5392..6171)
                     /gene="trpC"
                     /locus_tag="VMZ02_02285"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HUR70784.1"
                     /translation="MAEILATIAAHVREVVAMRRRDRPLQALRDRSIFGAPTRGFARA
                     VAGTQRRIVAEVKKGSPSKGLIRADFDPVAIAKDYAVHGASAISVLTEERFFLGDLHD
                     LEQIHANVALPLLRKDFMLDSYQIVESKSFGADAVLFIAAMLDNQLMSELRQQASELQ
                     MDSLVEVHSEAELEVALNVGAQLIGINNRDLKTFAVSLATTERLAPLVPAGIPAVCES
                     GIDSIEQIRRVESLGVHVFLIGESLMRAPEPGLKLANLLNG"
     gene            complement(6187..7203)
                     /gene="trpD"
                     /locus_tag="VMZ02_02290"
     CDS             complement(6187..7203)
                     /gene="trpD"
                     /locus_tag="VMZ02_02290"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HUR70785.1"
                     /translation="MNIREAIDKLVNRINLTELETVDVINQIMTGEATPLQVASFLTA
                     LRMKGETVDEITGAARVMREKAHRVNVGAITVLDTCGTGGDQKGTFNISTTAAFVVAG
                     AGVSVAKHGNRSVSSQSGSADVLAALGVKVDAPKERVEQCIAKIGIGFLFAPLLHEAM
                     KYAVQPRRDIGIRTIFNLLGPLTNPAMATHQLIGIYSADLVGMVATVLRNLGSARAMV
                     VHGLEGLDEISLAGPTKVAELRDGTITEYQIEPEKVGLNRCTLTALHGGSPEESSAIV
                     RAVLDGKSGAPRDVVLLNGGAALYISGSAASLQDGIKLAAESIDSGKARQKLRQLVEL
                     TNAV"
     gene            complement(7221..7916)
                     /locus_tag="VMZ02_02295"
     CDS             complement(7221..7916)
                     /locus_tag="VMZ02_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HUR70786.1"
                     /translation="MAENRRGKDSRYYLSRGQMIVLGGTFTLASVVIFLIGMMVGKVI
                     EERKILKRDEPLVKIPVKPAGPASGPAAKNDEITFYDTLAKSRSGQALAEQPSNEAKP
                     AEKNSSAEENETKVQVPVKPRTQSAKLTENKEVKPPPVAESAKNDSSDGSDGKIWRAQ
                     VDAFPDERSAKLLVDRLKNKGYNAYVSEVQFRGKPWYRVSVGKYGAREEADKVVNALK
                     GRENYAKAFAASK"
     gene            complement(7948..>9141)
                     /gene="argS"
                     /locus_tag="VMZ02_02300"
     CDS             complement(7948..>9141)
                     /gene="argS"
                     /locus_tag="VMZ02_02300"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HUR70787.1"
                     /translation="VNDAGNQMDNLGLSLYARYLEKLGQPAEFPETGYPGDYVKDIAA
                     EVVARDGDKYLQQNKTEAVDFFRRYASDALLGTIRGQLSDFGIRFDSYFSEKAMRERS
                     EVTQSMAALRERGLVYSQDGAEWFRSTQFGDDKDRTVIKSDGELTYFAADIAYHRNKF
                     ERKFDKLINVWGADHHGYVPRLKAAIEGLGYDANALKVVLVQMVQLTRGGEPVRMGKR
                     TGEFVALEDVLQEVGRDAARFFFLMRKSDSHLDFDLELAKKQSSDNPVFYVQYAHARV
                     ASIFDQAAKSGVALGATESVPVERLVLPQELELIRQMIQFNDVLEESVRDLEPHRMVF
                     YLLEFAGEFHRYYNLYRVVSDDIDLSRARLLLLANIQKTIRKGLDILGVDAPMKMTAR
                     PETEA"
BASE COUNT         1996 a         2705 c         2396 g         2044 t
ORIGIN      
        1 cgacgctcaa ggatccatta acgacggaag cgattttaaa tggtagcact gcttccggcc
       61 gcaaatcgaa acggagacca tccccgcggt cgcgccgaag ataattcgtt ccatccgccg
      121 accaacgcag ctccaacgga attcccgaga aaaaccggcg tccccaaaac gccacctgcg
      181 gagtccgcaa aaacgtgctt ttatcgggct gaataaagtc ctggtaaaaa ttaaactccc
      241 cctttaaaaa agtatcgctc cagcttttga acaaacccat gcgcgattcg ctatagcggc
      301 tgcgccgaat aattccctcg tcgccgaacg gcaagtcgaa tcgccgtatc agttcacgcg
      361 taaacaagtc atcgctgtat acggcggtat cactgtaggt aagccaatcg gacgctgtag
      421 tataacgatg cgagccgaaa acattccaac ggtctaaggg aattttcggg tcagcgatcg
      481 ttgaatcggc caccgatcgc gtggtccgcc aaccttcatt gaaataggaa gattcgaact
      541 tgaggtcgga gttgcggtcg aaaaccgtgc gatattcgcc gatgacgccg tagcgcgtcc
      601 gcgtctcgag gtcaaacgat aacgtcgcgt ccgagctttt cgaaattgcc caaaaaaatg
      661 gttgaagaaa tcgaaaccca tctttgctcg aagtaccgat gcttgggaat agcaaaccgg
      721 tctgacgctc agtgcgtagc ggaaaatatc cgtatgggat atagagcacc ggaacgtcca
      781 tgatatagaa aacgccgttt ttgatcgtac cggttccctc gagcgctaag tccatctgct
      841 cggcgaaaaa cttccacgat ggcgcgccgc tttcgcacaa acaggtggta aagaatccgt
      901 cgtcaatgtg gtaagactgg ccgccgaact tttcaaagcg gcggcccgaa acgctgatat
      961 gcccctgctc caggaaaagg tcggcgttgt gcaactcgcc ggtttcgttt tccatgttca
     1021 tgcgcagcga ttcggccgac ttgaccttcc attcggggtc gtcgagcgtc acccggccct
     1081 tggcttcgac ttcctgagtc gcgcggttca ttcgcagttc gtccgcttta agagttgtcc
     1141 cttctcgttt gacttcgaca ttgccagtcg cttcgatctg agttccgctg tcagtcgacg
     1201 acaacttgtc ggcctgcagg ttgatcggcg ccgcgggatt gttcggctgc gctgccccgg
     1261 aataagaagt ccacggcgcc actaccagca gcgcggtcat tatatagaac agttgaaaaa
     1321 tcatcgtgcg tacaactgcg cttcagccgt tgggttaggg ctttggcgcg gctatttctt
     1381 gcaccatcgg acggatctcg ccgagaagat aaagtgaacc tgctacgacg ataacatcgt
     1441 cagggccgga ttcatcaatc aatattcgca gcgccgcctt ggagtcaggg ttccatcgca
     1501 cagcaattct gcccgcgcag tgcctggcca actcggccgg gtccgtgctc cgttccatgg
     1561 caaccttggt aaacatcgct tccgacacca ccccgataag ccgcgtcagc attaggcgcc
     1621 agtctttgtc cgacatcgcc gcgaacagca atttaatttt gcgcccgcga taactctcga
     1681 gagcttcaac cagggccaga atcccttcgc cattgtgcgc cccatcaatg acgaccgtcg
     1741 gcctctccaa catcacttcc aaccttccgg gccattgcac agtcgccaag ccggcgcgga
     1801 tcgcgttctc ggctacgggg aagtaactgt caacggcttc caagccggcc agcgcaacgc
     1861 aagcattgta acgttgatgc aaccctcgca ggcgcaggga aagcccggaa taatgttgtt
     1921 ttactcctgt atagtcgaaa agcgcttcgg gtttcaagga gaaattaaaa tctgcaccaa
     1981 agaaatagac tttcgactcc tgctccttgg cgcggcggcg aaacacctct tttacttcgg
     2041 cggtgagcgc gccacagacg accggtatgc cgggcttgat aattccggct ttctcgccgg
     2101 cgatcgacag gagatcggcg ccaagaaaag cttcgtgatc tttggaaatc gtcgttatca
     2161 gtgaaaccag cggagtgata acattcgtgg cgtcataacg gccaccgaga ccgacctcga
     2221 cgaccgcaat atcgatcgag cgccgggcaa aataaacaaa tcccattacc gtcaccactt
     2281 caaaataggt caaggcgatc gctgcactcg cggtgcgctg ccagatctca tccgcgatcg
     2341 caacaacttc gtcttgggtg atctcctcat cacctatctt gatgcgctcg gtaaacgaca
     2401 ccaaatgagg cgacgtatac aaacccgtgc ggtatcccgc gacacaaaga attcgttgaa
     2461 gcatcgccga ggtcgagcct ttgccgttgg tgccggcgac gtgaatggaa gggaagtttc
     2521 tttccggatg gccgaaagtt tttaacgctg cggcgactct gtcgaggcgc aggtcgatga
     2581 tgccaccgcg aaggttgtag atccgatcaa gagtttcaga atagctggcc atgcggtggt
     2641 cagttccgag cctacaggcg catcataggc atcttggcaa ttaggttttt ttgcgggcgc
     2701 gcttcatcgc ccgtcttagt tgggctacga attccccggc ttgcctcacc ttggcagatt
     2761 tgctttttcc ctgctcgatg cgttgcacca aagcactgcc gaccacagca gcgtcggcga
     2821 actccgcgat ccacgccgct tgcttcggcg tcgaaatgcc gaagcccaca cctaccggca
     2881 gcgacgtcgt tttgctgacc tgagcaacct ggcggcgcaa atcaccatcg aaggactgcc
     2941 gtgcgccagt gacacccgtt actgagacgt aatagacaaa gccgcgccct tggcgggcga
     3001 ccaagcggcg ccgatccgcg ccgctggtag gagataagag aaaaatgacg tctaaatcgc
     3061 agcgatccgt gcaagtcttg agctcaccgc tctcttccgg tggcagatcc acgcagagca
     3121 cgccgtcggc ccccgccagc gccgcttgag tggcaaagtt atcgagaccg aagcgaaaga
     3181 atggattgta ataaccaaac aaaatgaccg gcaattcgga actgcgtcga aactcgcgca
     3241 ccagccggaa aattgcccgc aaggaaatgg tatttttcag cgcccgctcc gacgaacgct
     3301 gaatcgttgg tccatcggcc gagggatcgg aaaacggaat acctaattcg atggcatccg
     3361 ccccgccttc ttctaaggcc cgcataatct taagtgtggt ggccaagtca gggtcacccg
     3421 cggtgataaa aggaatcaac gcggcctcgc cgcgctgttt caaagcagca aatgttttct
     3481 gaatacggcc cataaaaaat aactaaccgt tcaggctcaa tgccgactca aatctctgcc
     3541 cgattgaaca actgaactga acaatcgcta attacaactg agctccgaga tggtcgccga
     3601 cggtgatcat atctttgtca ccgcgcccgg acaggttgac aataatgatc tgccgttttt
     3661 ttagtttggg tgccaaacgt cgcacgacag cgatggcatg agcgctttcc agggccggaa
     3721 tgatcccttc gacctgtgca agaaagcgga tcgacgcaat tgcctcatcg tcggttgctg
     3781 aatcgaactg tacccggcca tgatcgcgca agtaactcag ttcagggccg actcccggat
     3841 aatccaagcc cgccgcgacc gagtgggttt ccttaacttg gcccttgcca tcttgcagca
     3901 ggtagctctt gctgccatgt agtaccccca cggcgccgcg ctgtatcgtc gccgagtgtt
     3961 tgccggatcc aagtccgtgg ccggcagcct cgatcccgag cattttgacc agcctgtctt
     4021 taacgaacgg ataaaacagc cccattgcat tgctgccgcc gccgacgcaa gcaagcagat
     4081 gatcgggcag ccgtccttct ttttgcagaa tctgccggcg cgcctcgcgg ccgatgaccg
     4141 attgaaaatc tcgcaccatc ataggatagg gatgcggtcc ggccaccgaa ccgatcaggt
     4201 agtaggtggt ccgtacgttg gtcacccagt cgcgcaacgc ttcattcatg gcgtccttga
     4261 gcgttcggct gcccgatttc accacacgca ctttggcccc cagcagtttc atgcgaaaga
     4321 cgttaagcga ctggcgctga acgtcttctt cacccataaa aacctcacac tccatgccga
     4381 aacgtgctgc taccgtggcg gtggccacac catgttgtcc ggcgccggtc tcggcgatga
     4441 tacggcgctt gcccatgtgt cgcgccagga gaatttgccc catggtgttg ttgatcttat
     4501 gcgcaccggt atgacataag tcttcgcgct ttagatacac cgttgcgccg cccaggctgc
     4561 gcgtcagccg ttcggcgaaa tagagcggtg tctcccggcc gacatactct tttaggtaat
     4621 atgccaattc gcgtttgaat ccgccgtccc gccgcacttt tgcatagctg cgctccaatt
     4681 cggtaagcgc gggcatgagg gtttccgcga cgtaacgacc accgtactga ccgaaatgac
     4741 cgcgccggtc aggcaccttt ggcagcatcg atgaattcct taagtttccc atgatctttt
     4801 acaccagggc gagattccac tccgctgcac acgtcgacac cgaacggttg aacagtgcgg
     4861 atcgccttgg cgacgttgtc gactcgcagc cctccagata gcagaatctt cctcgcatcg
     4921 atcccttcga gccattccca agggaacatc tggcccgagc cgccgtatcc ggaggaaaaa
     4981 gaatcgacca gatagtagtc agccgggaag cctttcaagg cgtcgagcgc agcttgctcc
     5041 ttgatgcgaa acgccttgat cacttttaca tcccagcctt gacagtacgc gccattttct
     5101 tcgccgtgaa actgcaaagc gcgatagcca tcgtggccat tccgtgatcg agccgccgcc
     5161 aagacctcgc tcacccgggc ttttgtctca ttgactagga gtgccacatt gaacgcatcg
     5221 cgcggcaagc gggcgattat ttctccagcc aagtccgggg taatgcagcg cgggctcggt
     5281 ggatagaaat taaatcccaa aagatccgcg ccaaactcga gcgacttctc ggcgtcatct
     5341 aggttggtaa ttccacagac tttgatcttg accggcatcg acacgcaact ttcagccgtt
     5401 taataggttg gcgagcttga ggcccggctc gggcgcgcgc atgagcgact caccgatcag
     5461 aaacacatga acacccaacg attcaactcg acggatctgt tcgatactgt cgatgccact
     5521 ttcacaaacc gccgggatac ctgccggcac cagtggcgcc aatcgttcgg tggtcgccaa
     5581 gctgacggca aacgtcttca ggtcgcggtt gttgatacca atcaattgtg cgccgacgtt
     5641 tagggccact tccagctcgg cttcggaatg cacttcaacc agcgagtcca tttgcaattc
     5701 actggcctgc tggcgcagct cgctcatgag ttggttatcg agcatcgccg cgataaatag
     5761 caccgcatcg gcgccaaaac tcttggactc gacgatctga tacgaatcga gcataaaatc
     5821 cttgcgcaac aacggcagcg ccacattcgc atgaatctgt tcgagatcat gcaaatcccc
     5881 taaaaaaaac cgctcctcgg tcagtaccga tatcgcactc gccccatgga ctgcatagtc
     5941 tttggcgatg gcgaccggat cgaaatcggc gcgaatcaac cctttcgagg gcgaaccttt
     6001 tttgacttca gcgacaatcc ggcgctgcgt tccggctaca gccctagcga atccgcgcgt
     6061 cggcgcgcca aagatcgaac ggtcgcgtaa cgcttggagc ggccggtcgc gccggcgcat
     6121 tgcaacgacc tcgcgcacat gcgccgcgat ggtggcgagg atttcagcca tggaactatt
     6181 atacagctat acagcgttgg ttagttcaac cagctgtcgg agtttttggc gtgccttgcc
     6241 gctgtcgatg gattctgcgg cgagtttgat cccgtcctgt agactcgcgg cgctgccgct
     6301 aatgtaaagc gccgcaccgc catttaatag gacgacatcg cgcggcgcgc cgctcttgcc
     6361 gtctaggacg gcacgcacga tcgcggagct ctcctcgggg ctaccgccgt gcagcgccgt
     6421 caaggtgcat cggttgagac cgactttttc tggctcgatt tggtattctg tgatggtacc
     6481 gtcgcgcagc tccgctactt tggtcggccc ggccagagag atttcatcca agccttcaag
     6541 gccatgcacc accatggctc tcgcgctacc gaggtttctg agcacagtgg caaccatgcc
     6601 gactaagtcc gcgctgtaaa taccgatcag ctgatgcgtc gccatggccg ggttggtcag
     6661 tggaccgagt aagttgaaaa tcgtgcgaat gccaatgtcg cggcgcggtt gcacagcgta
     6721 cttcatcgct tcatgaagca gcggcgcaaa caaaaacccg ataccgattt tagcgatgca
     6781 ctgctcgacg cgctctttgg gcgcatctac cttgacgcct aatgcggcca aaacgtcggc
     6841 gctgccggat tggctggata cggaacggtt gccgtgcttt gcgacgctga cccccgcgcc
     6901 tgcaaccacg aatgcggcgg tcgtcgaaat attgaacgtg cccttttgat caccgccggt
     6961 gccgcaagtg tcgagcacag tgatggcacc gacgttgacg cggtgcgcct tttcgcgcat
     7021 gacgcgggcc gctccggtga tttcatcgac ggtttcgcct ttcatgcgca acgcagtcag
     7081 aaaggacgcg acctgcagcg gcgtcgcctc gccagtcatg atctggttta tgacgtcgac
     7141 cgtttccagc tcggtaaggt tgatgcgatt gacgagctta tcgatggctt cgcgaatgtt
     7201 catggcctag attgcgcggc tcacttggac gcggcgaatg ccttggcata attttctcgg
     7261 cccttgagcg cgttcaccac tttatctgct tcctcacgcg caccgtactt gccgacactc
     7321 acccgatacc agggcttgcc gcgaaactgg acttcactga cataggcgtt gtagccttta
     7381 tttttcagtc gatcaaccaa cagtttcgcc gatcgctcat cgggaaatgc atccacttgg
     7441 gcacgccata ttttcccgtc actgccatcg ctcgagtcgt ttttggcgct ctcagccact
     7501 ggcggcggtt tgacctcttt gttttcggta agcttggccg actgcgtacg tggtttgacc
     7561 ggtacctgga ctttcgtttc gttctcttcg gcgctggagt ttttttcggc cggtttcgct
     7621 tcgttgcttg gttgctccgc gagtgcctga ccactgcgtg acttggccaa ggtatcgtag
     7681 aacgtaattt cgtcgttttt tgcagctggg ccgctggccg gaccggcagg tttgactggt
     7741 attttcacca agggctcgtc ccgttttagg atcttgcgct cttcaatcac cttgccgacc
     7801 atcatgccaa tcagaaagat caccaccgac gccaaagtaa aagtgccgcc cagaacaatc
     7861 atttggccgc gactcagata atatcgactg tctttgcccc tacgattctc tgccatctgg
     7921 attaccctct cggcccgtta tcactgatca agcttcggtt tcgggtctag ctgtcatttt
     7981 catgggtgcg tcgactccga ggatgtccaa tccttttcgg atcgtctttt gaatgttggc
     8041 gagcaataac aatcgcgccc ggctcaggtc gatatcatcg gacaccacgc gataaaggtt
     8101 atagtaacga tgaaactctc cggcaaactc aagtaaataa aacaccatgc ggtgcggctc
     8161 gaggtcgcgc accgactcct ccaagacgtc gttgaattgg atcatttggc ggattaactc
     8221 caactcctgg ggcagtacca gacgctcaac gggcaccgac tcggtggcgc cgagagccac
     8281 gccgcttttt gccgcttggt cgaaaatact cgccaccctc gcatgggcgt attgaacata
     8341 gaatactgga ttgtcgctcg attgtttttt tgccagctcg agatcgaaat caagatggct
     8401 gtccgacttg cgcataagaa agaaaaaccg cgccgcatcg cgcccgacct cctgcaggac
     8461 gtcttcgaga gcgacgaatt cgcccgtacg cttgcccatc cgcaccggtt caccgccgcg
     8521 ggtgagttgg accatctgga ccaaaaccac tttcaatgcg ttggcgtcat aacccaaccc
     8581 ctcgattgcc gcctttagac gcggcacata gccatgatga tcggcgcccc agacattgat
     8641 caatttatcg aacttacgct cgaatttgtt gcgatgataa gcgatgtcgg ccgcgaagta
     8701 ggtcaactcg ccgtcgcttt tgattaccgt gcggtccttg tcgtcgccga attgagtgga
     8761 gcgaaaccac tcggcgccgt cctgactata aacaaggccg cgctcgcgca acgccgccat
     8821 tgactgggta acctcgctgc gctcacgcat cgccttctcg ctgaagtagg agtcaaaccg
     8881 gatgccgaag tcgctcaact gaccgcgtat ggtgccaagc agcgcatccg acgcatagcg
     8941 gcgaaagaaa tccaccgcct cagttttatt ctgctgcaga tacttgtctc cgtcccgcgc
     9001 tacgacttcg gcggcaatgt ccttaacgta gtcaccggga taaccggttt cgggaaactc
     9061 cgcaggttgg cccaattttt ctaagtagcg ggcgtaaagc gagagtccga gattgtccat
     9121 ctgattaccg gcgtcgttga c
//
LOCUS       DATLCH010000080         5553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5473303, whole genome shotgun sequence.
ACCESSION   DATLCH010000080 DATLCH010000000
VERSION     DATLCH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5473303"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..861)
                     /locus_tag="VMZ02_02305"
     CDS             complement(163..861)
                     /locus_tag="VMZ02_02305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR70788.1"
                     /translation="MAERIVSMHAAHRSGKDVVDGFLSRPTTGGERSAIVLVHGYRGL
                     DDGQRAVTRRFAQEGFVCLSPDLFHGKTYHTLEDCALKKTSLDIPRAVNHIVDSVPYL
                     RKLPSVGKKKVAVLGFCMGGGLALYALAQSKVFDAGVIYYQSMFPDPEDLKSITAPML
                     CHYGTDDSGTTQTEIEMFRESLERHKKKFQIEMYPGAGHAFLNNPSSKNAANRDAADK
                     SVASTAKWLKKVLA"
     gene            complement(894..1508)
                     /gene="leuD"
                     /locus_tag="VMZ02_02310"
     CDS             complement(894..1508)
                     /gene="leuD"
                     /locus_tag="VMZ02_02310"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HUR70789.1"
                     /translation="MSEIIKIVKVTGTALPMRGNEIDTDRIIPARYLKEVSFARMGDY
                     PFFDERYTSDGKKKDHPFNDPEYSGASILFVNKNFGCGSSREHAPQALNRFGIKVIVG
                     ESFAAIFAGNCTMMGVPTVTVSQQDMQQLMKSVEENPKIEYTVDIENKTLTYGKVKVA
                     IDLPDTYRTALTMGSWDSTALLRANLDRVKETAKKLPYMNNFAS"
     gene            complement(1520..2917)
                     /gene="leuC"
                     /locus_tag="VMZ02_02315"
     CDS             complement(1520..2917)
                     /gene="leuC"
                     /locus_tag="VMZ02_02315"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HUR70790.1"
                     /translation="MAERTLYDKIWQSHTVGILPTGQTQLFIGLHLIHEITTAPAFDM
                     LREKGMDVAFRDRTFATVDHIVPTDLRQRPFKDQEAEDLTQALERNVNQFGVEFFGLD
                     SMNQGIVHVIGPQLGLTQPGMTLACGDSHTSTHGAFGTLAFGIGTSQVRDVLATQTLA
                     MDKLKVRRINVNGALATGVYAKDVTLHIIRQLGVGGGKGFAYEYGGDVIDRMNMEERM
                     TVCNMSIEGGALVGYVNPDQTTFDYIKGRPYAPKGEAFDRAVKYWQTIASDADAKFDD
                     IVNFEGKDIEPTVTWGINPGQSVGVSEKLPLPDNSNDPESAKKAYEHMGWTPGAPIIG
                     TPVNVAFIGSCTNGRLADFRAAAKIAKGRKVNPNVRALVVPGSAEVKKLAEAEGLDKI
                     FEEAGFQWREAGCSMCLAMNPDKLNGREVSASSSNRNFIGRQGSPQGRTLLMSPAMVV
                     AAALNGKVVDVRQYE"
     gene            complement(3147..3986)
                     /locus_tag="VMZ02_02320"
     CDS             complement(3147..3986)
                     /locus_tag="VMZ02_02320"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HUR70791.1"
                     /translation="MNSRSVGRLHRGSSGKHSDLRLIISRSIASIVTLFLANLVFITG
                     CTSGENRAVSSDWNAQRQRMINEQLRTRGIHNARVLDAMLAVPRHLFVPEPDRTRAYG
                     DFPVAIGHGQTISQPYIVAFMTQELAIESAHRVLEIGTGSGYQAAVLGLLAKEVYTLE
                     IVASLAERARETLATLGYHNIAVRIGNGYLGWPEHAPFDRIMVTAAPDEVPAPLVQQL
                     KIGGLMAIPVGTVTQQLRILRRTSAGMEPLKILPVRFVPMTGKPDNRGSRATSPVGDP
                     REK"
     gene            complement(4126..5037)
                     /locus_tag="VMZ02_02325"
     CDS             complement(4126..5037)
                     /locus_tag="VMZ02_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUR70792.1"
                     /translation="MMPQHDNFYDVYSKAKKIKIKWPQGARMAIALAGNLEAWTEIPN
                     AKHRRTHHVGGSNPIKEDDVVSQYDCRTASENDYGGRTGIWRILRILDKYGVKASFNT
                     NALCIHRYPEAVRAVHQRGHEIVGHSYAEDIQLTQLTADGERDEIRTCAKMFEEFTGV
                     RPTGWLTSGMRHTERTLGILADEGFAWHGDAVNDDNPYPAVVNGKTMIIVPYKNAVSG
                     LNDTGMYRRGITARDVFNSFKDEFDLLYEESQTEPKMLTLAMHCQMAFPATGKVYDDC
                     IKYAKSFPDLWFAKRCEIVEWVKKHCM"
     gene            complement(5112..>5553)
                     /locus_tag="VMZ02_02330"
     CDS             complement(5112..>5553)
                     /locus_tag="VMZ02_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HUR70793.1"
                     /translation="RRDGRSRLYEYRIFNRATPSPFHRHRAWHIHEPLDVDAMAKAIE
                     CLIGEHDFSSFRAANCDATHPVRTIYHTHIERRGELLVYTIEGTAFLRHMVRNLVGTL
                     ADVGRGIRKPQSFAELLDARDRTKAGYTAPPEGLYLVQVNYDRA"
BASE COUNT         1172 a         1573 c         1487 g         1321 t
ORIGIN      
        1 atggaggagg tcttcttcac cgcttctttc gcttcggctt cctgcttggc ctttagcttc
       61 gccagctgct cccgttcctt cttgacgaaa tactcgtcct cctgcgcttt cttgcgttca
      121 tcccacttgt tcgccatcac caacctccag aagtaaatct aactatgcca gcaccttctt
      181 cagccacttc gccgtgctgg caaccgattt gtctgcggca tcgcgattgg ccgcgttctt
      241 actcgacgga ttgttgagaa aggcatggcc ggcgcctgga tacatctcga tttgaaattt
      301 tttcttgtgc cgctccagac tctcgcgaaa catttcgatt tcggtctgcg tcgtgccgga
      361 gtcatcggtg ccgtaatggc acagcatcgg tgccgtaatg cttttgagat cctcgggatc
      421 gggaaacatg ctttggtagt aaatcacccc ggcatcaaag accttcgact gcgccaaggc
      481 atacaatgct agccctccgc ccatgcagaa gccgagcacc gcaactttct tcttgcccac
      541 cgatggcaat ttgcgcagat aaggcacact gtcgacgatg tggtttacgg cgcggggaat
      601 atccaaggac gtttttttga gcgcgcagtc ttcgagggtg tggtaggtct tcccatgaaa
      661 gagatccggc gacaggcaga caaaaccctc ttgggcgaag cgacgcgtca ccgctctctg
      721 accgtcgtcc agtcctcggt agccatgcac caatacgatt gccgatcgct ccccgcctgt
      781 cgtcggtcgg gagagaaatc catcaacgac gtctttgccg ctgcggtgcg ccgcgtgcat
      841 agacacgatc cgttcagcca tatttcacgt cccggtgctg aaaacgctgc gcttcaactg
      901 gcaaagttat tcatgtaagg cagttttttg gcagtctctt taacccgatc gaggttggcc
      961 cgtaggagcg ccgtggaatc ccatgatccc atggttaacg ccgtgcggta ggtgtccggc
     1021 agatcgatgg ctaccttaac cttgccgtag gttaacgtct tgttttcgat gtcgacggta
     1081 tattcgatct tgggattttc ctcgacgctc ttcatcagct gctgcatgtc ttgttggctc
     1141 acagtcaccg ttggcacacc catcatggtg caattgcctg cgaagatcgc cgcgaaggat
     1201 tcgccgacga taactttaat accgaagcgg ttgagcgctt gcggcgcatg ctcgcgcgac
     1261 gagccacagc cgaagttctt gttgacgaac aagatcgacg cgccgctata ctcgggatcg
     1321 ttgaacggat gatctttctt ttttccgtcg ctggtgtagc gttcgtcgaa gaatggataa
     1381 tcgcccatgc gcgcgaagct gacttctttc aaataacgcg cgggaatgat ccggtcggtg
     1441 tcgatttcgt tgccacgcat tggcaaggcc gtacccgtga ctttgacgat cttaataatc
     1501 tcgctcatgt tgtcctcagc tactcatact gacgcacgtc gacgactttg ccattgagcg
     1561 cggctgccac caccatggcc gggctcatca atagggttcg gccttgcggg ctaccctgcc
     1621 gaccgatgaa attacggttc gatgacgacg cggaaacttc ccggccgttg agcttatccg
     1681 gattcatcgc caagcacatt gaacagcccg cctcacgcca ctgaaatccc gcttcttcga
     1741 agatcttgtc caggccttct gcctcggcaa gtttcttcac ctcggcagaa ccgggcacta
     1801 ctagcgcgcg cacgttggga tttacctttc gccccttggc aatctttgcc gctgcacgaa
     1861 aatccgcgag ccggccgttg gtgcaagaac cgataaacgc aacgttcacc ggagtgccga
     1921 tgatcggtgc acccggcgtc cagcccatat gctcgtaagc cttcttggcg ctttcggggt
     1981 cgtttgagtt atccgggagc ggcagttttt ccgagacccc caccgactgc cccgggttta
     2041 tgccccaggt caccgtcggt tcaatgtctt taccttcgaa gttcacgata tcgtcgaatt
     2101 tggcatcggc atcggatgca atggtctgcc agtacttgac cgctctgtcg aacgcctcac
     2161 ctttgggcgc atagggccgg ccttttatgt agtcgaacgt agtctggtcg ggattgacgt
     2221 aacccaccaa ggcgccgcct tcgatgctca tattgcacac ggtcatgcgt tcttccatgt
     2281 tcatccggtc gatcacgtca ccgccgtact cgtaagcgaa gcccttgccg ccaccaacac
     2341 ccaattggcg aatgatgtgc agcgtcacat ccttggcgta aacgccggtg gcaagtgcgc
     2401 cgttgacatt gatacggcgg actttcaatt tgtccatggc cagagtttga gtcgccaaga
     2461 cgtcgcgcac ttggctggtg ccgatgccga aagccaaggt gccaaaggct ccatgggtgc
     2521 tggtatgact gtcgccgcat gccagcgtca tgcccggctg tgtcaagccc aactgcgggc
     2581 cgatgacatg gacgatgcct tggttcatgc tgtcgaggcc gaaaaattcc acgccgaact
     2641 gattaacgtt tcgttccaaa gcttgagtaa gatcttccgc ctcttgatcc ttgaatggcc
     2701 gctggcgtag gtcggtagga acaatatggt ccacggtcgc aaacgtccga tcgcgaaacg
     2761 ccacgtccat acctttttcc ctgagcatgt cgaacgccgg cgcggtggtg atctcgtgaa
     2821 ttaaatgaag cccgataaag agttgagttt gaccggtcgg cagaataccc accgtatgcg
     2881 attgccaaat cttatcgtaa agagtccgtt cggccatcaa gaatttctcc ttcggcgggg
     2941 ataatatcgg tcggtatctg ggacttcaag agctcgaata gcaaagacaa tcccgattca
     3001 gcggagacga gaatagctta cgagacacca gtgtatgggt cgagacggct gctcttctgg
     3061 agtgatgaaa agagccgcgc ggcacttacc tgaacggaat ctgatccgat taccttgaac
     3121 actaggcagc cattctaaag ccgccgctat ttctcccgtg gatctcctac gggacttgta
     3181 gcacggctgc ccctattgtc aggcttgcca gtcatcggca caaaacgcac aggcaggatt
     3241 ttgagcggct ccattcccgc cgacgttcgg cgcaaaatac gcagttgctg agtgacggtg
     3301 ccgaccggaa tcgccattaa accgccgatc ttcagctgct gcaccagggg cgccggtacc
     3361 tcgtccggcg ctgctgtcac cataatgcgg tcgaacggcg cgtgttcggg ccatccgaga
     3421 taaccattgc cgatgcggac tgctatattg tgatagccga gggttgcgag cgtctcacgc
     3481 gcccgttcgg ccaaagacgc gacgatctcg agcgtataca cctccttcgc gagtaaaccc
     3541 aaaacagccg cttgatagcc cgatcccgtg ccgatctcta acacgcgatg agctgattcg
     3601 atagccaact cctgagtcat gaaagcaacg atatatggct gtgagatcgt ctgaccgtga
     3661 ccgatagcaa cgggaaagtc accataggcc cgagtacgat cgggttccgg aacaaataga
     3721 tgcctcggca cggccagcat cgcgtcgagc acccgggcgt tatgaatgcc gcgcgtgcga
     3781 agttgctcgt tgatcatgcg ctgacgttgc gcgttccagt cgctcgagac agctcggttc
     3841 tcacccgaag tgcatccggt aatgaagacg agattggcga ggaaaagcgt aacgatcgac
     3901 gcaatactac gtgagatgat caggcggaga tcggaatgtt tgcctgacga tcctctatgt
     3961 agccgaccaa ccgatcgaga gttcactgtt acgtctcacg gtcctaattg taagtacttc
     4021 gttgcgaacg tcgtgatgtt gccaattggc aacggatgct ggatcagtga ttgttggatt
     4081 ccgcaatttc aaaaaaatta ctccgctgct ctattatccc agtccttaca tgcaatgttt
     4141 cttcacccac tcgacaattt cacaccgttt agcgaaccat aaatcgggaa acgatttggc
     4201 gtacttgatg cagtcgtcat agacctttcc cgtcgctgga aatgccattt ggcaatgcat
     4261 agccagcgtg agcattttcg gctccgtctg cgattcctca tagagaagat caaattcatc
     4321 tttgaaggaa ttgaaaacgt cccgcgcggt aatgccgcgg cgatacatgc cggtatcgtt
     4381 aagaccgctg acggcgtttt tgtacggcac gataatcatg gtcttgccgt ttaccacggc
     4441 aggatacggg ttatcgtcat tcaccgcgtc gccatgccag gcaaatcctt catctgcgag
     4501 gatgcccaaa gtgcgctcag tgtggcgcat gccggaagtc aaccagccgg tcggacgcac
     4561 gccggtgaac tcctcgaaca tcttggcgca ggtgcggatc tcgtcgcgct ccccgtccgc
     4621 cgtcaattgt gtaagttgaa tatcttccgc gtaagaatgg ccgacaatct catggccacg
     4681 ctggtgcacg gccctgactg cctcaggata acgatggatg cagagcgcat tggtgttgaa
     4741 cgatgccttg acgccgtatt tgtcaagaat gcgcagaatg cgccagatcc cggtgcggcc
     4801 gccgtagtcg ttttcactcg ccgtgcggca gtcgtactgc gacaccacat catcttcctt
     4861 gatcggattg gaaccgccga catgatgcgt gcgccggtgc ttggcgttgg ggatttcggt
     4921 ccacgcttct aaattacccg ccagcgcaat tgccatccgc gcgccctgcg gccattttat
     4981 tttgatcttt ttcgcctttg aataaacatc gtagaaatta tcgtgctggg gcatcatggg
     5041 ctggcctcct cctccaatga ccgaacagaa ttataccgca tgggtataag taaatcgccg
     5101 aggggtgtca attaggcacg gtcgtaattt acttgtacta ggtacagtcc ctccggtggc
     5161 gcagtgtatc cggccttggt gcgatcgcgc gcatcgagca actcggcaaa cgactgcggc
     5221 tttcgaatcc cacggccgac atcggcgagg gtaccgacga gattgcgcac catgtggcgc
     5281 agaaaagcgg tgccttcgat ggtatatacc aagagttcgc cgcgccgttc gatatgggtg
     5341 tgatagatcg tgcggactgg gtgggttgcg tcgcaattgg cggcgcggaa agatgagaaa
     5401 tcatgctcgc cgatcaagca ttcgatggcc ttggccatgg catcaacgtc caacggttcg
     5461 tgaatgtgcc acgcgcgatg acggtggaaa ggcgaaggcg ttgcccggtt gaaaatccga
     5521 tactcataca aacggcttcg gccatctcga cgt
//
LOCUS       DATLCH010000081        13875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10566301, whole genome shotgun sequence.
ACCESSION   DATLCH010000081 DATLCH010000000
VERSION     DATLCH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13875
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10566301"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VMZ02_02335"
     CDS             complement(<1..258)
                     /locus_tag="VMZ02_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70794.1"
                     /translation="MTHQQRLVATIAVLFLSIVVRVEPAGAQTKLTIGYAAVSPRTTP
                     LYLAQEQGIFGKYGLDPKIVLVRAAATLVATLVSGEMDVGYT"
     gene            complement(357..1199)
                     /locus_tag="VMZ02_02340"
     CDS             complement(357..1199)
                     /locus_tag="VMZ02_02340"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HUR70795.1"
                     /translation="MAKKKEYTCVLVETDDEGITWISFNRPEKRNAMSPTLHVEMEEI
                     LYGLETDEETKVIVLTGAGNSWSAGMDLKQYFRETDKDPVARFRAATAHRRWATELLT
                     WSRKPTIAMVNGYCYGGAFVSLAACDIVIAADNATFGLSEVNWGIIPGGNVSKVFGEM
                     VGFRNAMFYAMTGRTIDGRKAAEIGIATMAVPADRLREETITIARELMAKSPMVLAYT
                     KHAIRAVRGMDMDQAYEYLGAKSMALRAADPEHTRERGMREFLDEKTYRPGLEPVKRR
                     AEDK"
     gene            complement(1251..2123)
                     /locus_tag="VMZ02_02345"
     CDS             complement(1251..2123)
                     /locus_tag="VMZ02_02345"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70796.1"
                     /translation="MENIKIGIGTSSIGRFLPYVGEAAGLFKKQDIAVEIINQFDEEK
                     VVEDIASQVTPIGTPNAPSLIFSRLNGNDLIIIGGVLNRPAFYLVANSTIESIGSLKG
                     KRVGINQPRRMAGMMMLALLRRWRLDRDDDLELVDLGLNDRSYEALLSGTLDAALLPP
                     EKAFLAESAGLRIIADSLELESHWVPLATTRQFLDGNRQLVGAIAAIYRESIRLFNSD
                     MQFALNVIGRQLPTLRAHPQVIEKCYRVFAELFEPSLVAAPASLTAVIEEVARQDARA
                     NRLAAQDLVVEQGQ"
     gene            2385..3155
                     /locus_tag="VMZ02_02350"
     CDS             2385..3155
                     /locus_tag="VMZ02_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR70797.1"
                     /translation="MKPDQFIDELWSYAAEVPMEQHPWFDGIVKHRWTKEQIILGEVQ
                     HYLRVRTNPIFFGYMAINAVSAKEYTVMETVLENFMEELGGKRTHVDIMLQFLEEGGI
                     GREQADNAEPAPGTLAAIEMIIGCCQRRSALEGVAMLAFVESQLGGAGKISEKVYREL
                     TGHYGFSPRAAATYQLHAAQDEGHGSRQIDAIRLLATDEATQNKIRAAVKLGLTAFTL
                     EWDGHVQAMTGKREFWSGVKDLQLRQPIVNLSSQKSSV"
     gene            complement(3219..3350)
                     /locus_tag="VMZ02_02355"
     CDS             complement(3219..3350)
                     /locus_tag="VMZ02_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70798.1"
                     /translation="MVSNAVKQSQKEVVAALKRLRAQHSDDVEYQKLRKDLPDEWPI"
     gene            complement(3451..3864)
                     /locus_tag="VMZ02_02360"
     CDS             complement(3451..3864)
                     /locus_tag="VMZ02_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70799.1"
                     /translation="MRSKSEPCIALQQIAPARDVAEGCWQILWQVDNPGDGALFIVSV
                     HFPHGQFKSAVRHFTPALEVAAGASQRFESVIQCREPAGLVTENAFIIFHCQWRGEAW
                     RIFVRLRVDVDAAGAPHTATELITTQKVGFSGISN"
     gene            complement(4043..4492)
                     /locus_tag="VMZ02_02365"
     CDS             complement(4043..4492)
                     /locus_tag="VMZ02_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70800.1"
                     /translation="MSDFAKVVSVDPVIAESLFGSSARNGPAGAERELMLAVLSDAIE
                     CYWKYQKSAKRSAIRLYQDAKKWIFAEDDFQPFSFKDVCEILELEPAYIRRGILAGGR
                     AAPVMTGREEEDERRNIKRKLKSGRTWKGSSRLSRGRVRPLRVGPRG"
     gene            complement(4586..4942)
                     /locus_tag="VMZ02_02370"
     CDS             complement(4586..4942)
                     /locus_tag="VMZ02_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70801.1"
                     /translation="MEFRVKTAQFGQARCRLARAFLCVLPVFVLLQLIGQPELDDRNP
                     LYGPRSVSAKIFKHEQSPKSGLTQVLQLSLAVGSRLFTPGFYLTYVRNVRQQDFVRTP
                     LVSLRSSRSPPRIAAA"
     gene            complement(5057..6100)
                     /locus_tag="VMZ02_02375"
     CDS             complement(5057..6100)
                     /locus_tag="VMZ02_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HUR70802.1"
                     /translation="MAPTSYRSARFVSFLFLLSGGFCAGAEAQSLPRSVSVGSNPPGT
                     VFYALASGLSKVASEGAPFQMVVQPYSGTSTFLPLLDSGEVDFGINNAVDMALSYQGP
                     ERLKIGGRNPFQHTPNVRLVMRGAVLMVALLVRKDAPMKSVLDIRGKRLTGEYPAQLS
                     NWYNIFGFLAGAGMKWEDVKIVAVPGVNEGVDALMQGRADVSMSAIDAAKVKEADAAI
                     GVKHISLDCSAQGEKRLRAAVPGYYPYWIKRGGATAIVEDTCVNAYDIYLTSGKSAND
                     RVVQTILKNIWDNVEKLPPLHPSFKDWTRQRAVDAEVTIPYHPSAVQFYKERTVWTAA
                     MDEAQRKLLSLNP"
     gene            complement(6254..6844)
                     /locus_tag="VMZ02_02380"
     CDS             complement(6254..6844)
                     /locus_tag="VMZ02_02380"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HUR70803.1"
                     /translation="MAAGAGVCPKQFNIQSEQPGKGQVWTLFVALFVHANVLHLFGNM
                     LFLFVFGNTLEKTVGARKLLLIFFTGGLTGFILGVAYLPRATGMLGASAAIFALAGCV
                     MLVRPLKFSWLFLAPQGLIAVIYFVYNVIVVYEKSRIPGYDPQVAYFAHIIGFIVGLP
                     FGIALSDHWKRNLLITLVLFGIYVMLVSGAATPLFR"
     gene            complement(7441..8238)
                     /locus_tag="VMZ02_02385"
     CDS             complement(7441..8238)
                     /locus_tag="VMZ02_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR70804.1"
                     /translation="MTHSSSTNGSYADLPGVRLWYTDSGGSGIPLILLHANTGNADGW
                     QYNIPGFAEAGYRVIAFDRRGWGRSVANPDTGPQPGTIADDLHALTEFLKLDRFHLVG
                     VAGGGFAAYDYVLWHPEQLRSLVVAASGGAIVDEALSKIRERTALPNFRNWPPEFREV
                     SMGYMATNPEGLKLWLEIHDNSIQKGASAQPQRQTITFAKLATIRVPTLLMPGDQDLQ
                     APPWVMRWQLAQIPGAEFIVLPEASHSINWEQPEAFNRNVLEFIRRH"
     gene            complement(8235..9167)
                     /locus_tag="VMZ02_02390"
     CDS             complement(8235..9167)
                     /locus_tag="VMZ02_02390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HUR70805.1"
                     /translation="MQYGLHLPASSATVASEELIRFAQRAEALNFSLLTVADHVIVPT
                     NISVPYPYTVDGKYPGTGFHLETLVTMSFLAGATQRIRFATSVMILPYRNPIVTAKML
                     ASLDVLSGGRVIVGVGVGWMKEEFENLQAPDYAARGRVTDEYIGAFRELWTADRPTFK
                     GRFVNFSDLIFLPKPVQKTIPIWIGGHSKAALKRAGELGDGWHPIGGVPTIPLEPADI
                     TKDLVTVSEHAVKAGRDPKTIHVALKGSLFDKEKKIADRRRRFMGGAEEIANDIREYA
                     AAGVNTMIFDVRRPTSAETLERMEWMAQEVYPKV"
     gene            complement(9339..9905)
                     /locus_tag="VMZ02_02395"
     CDS             complement(9339..9905)
                     /locus_tag="VMZ02_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HUR70806.1"
                     /translation="MTQAIRPMPEEMPPELWRGYQPADRAWQGSIKEIFITPASAQAM
                     VAVPRVRAFADRGLEGDRFYRESWNAVKRSDKAVSLIEDEVLELAGKEIGVDAIGILT
                     RRNIVTRGVPLIELLGREFTIGNVRMRGIRLFEPCGHLVAVTKIPAIFRALEHRSGLK
                     AAVLSDGEIQIGDAVVLASASAAVATAG"
     gene            complement(9984..10466)
                     /locus_tag="VMZ02_02400"
     CDS             complement(9984..10466)
                     /locus_tag="VMZ02_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70807.1"
                     /translation="MEPAKKLAPTEKPRTQKFSLRTPYMKQGRITQLVAETENMWIHT
                     KINYEGGENEIHTHLDEDHSFIVLEGQMSVFDEKGGEIKVGKHQGVMIPRGAYYRYLN
                     TGTENLVVLRVGSGIKGKAQGGEEMRVRPDGKPLIASSEENKTVPPVEDSRFYADEAG
                     "
     gene            complement(10491..11552)
                     /locus_tag="VMZ02_02405"
     CDS             complement(10491..11552)
                     /locus_tag="VMZ02_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70808.1"
                     /translation="MTTAAGVKRTAEQVLGQDKIVSSDSHIMEPEDLWLKNLPPSLAA
                     KFPKFPPRNTAGEKPGGHDPKARLDEMAVDGVSAEVLYATYGLRLYAMEDAPLQEACF
                     EIANDWMMEYCKVAPNRLFGIPMISMYNIPNAIKELERCKKNGMIGCMIWQVPPDHLP
                     FTSDYYNPFWEAAGELDMPVNVHILTGFNYSRFDRKGLDTYRMTVNQKLTDAANTLFD
                     FVFGGPLAKFPKLKLVYVENEVGWIPFYCHEWDKYYVRHSPKAPLPYMKKLPGEYVKD
                     QVFATFFSDPAGGRMMDWFGEDNFMWSSDYPHAASTWPHSRQVITDELGHLPKNVIDK
                     VVRQNVLDLYHIKVDGINN"
     gene            11679..12515
                     /locus_tag="VMZ02_02410"
     CDS             11679..12515
                     /locus_tag="VMZ02_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR70809.1"
                     /translation="MIRAIKRYLCNCLSILLIPASLFAAEAQNAFNEGVKLYKSAKYK
                     EAVAAFNRAIKAAPKSDEAYNNRGLAYAKLGQQDNALKDYDEALKLNPTLTDAQFNRG
                     NIFFTQKQYERAVKDFDEVIKQDPKAPRVFVQRGLALLNLKQFDKAVSDFDQAQKLDP
                     KDIEIYSNRGLAHRRLGKNDLALLDYSEAIKLDPKNGENYNNRANAYSLLGKYDEALK
                     DYDEAVKLNAKDARIYANRGVIKQRLKRDDEAQKDFQKALELDASLKDKLAPFLGPLK
                     GK"
     gene            12519..13697
                     /locus_tag="VMZ02_02415"
     CDS             12519..13697
                     /locus_tag="VMZ02_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR70810.1"
                     /translation="MLRVADVWQLTLRSPRLPFIGRAQRSILALLLSCALKSLVPINA
                     IALVTLAGIIGPLNGAAPAMAGLEEPLKTALELHESGKIKEAIAEYDKVIKANPKMAE
                     AYFNRGNAYFDLGQNQQALKDYSEAIRLNPKDAEAYFNRGNTYRRLKQDPAALNDFTN
                     AIKLNPDDAKSYTNRASIHFDARRYEAALKDQNEALRIDPKDAQHYFNRGNSYLALDK
                     KDDAIKDYDAALSRDPKLADAYYNRGIVYADALKYDNAIRDFDEAIKLNPKNPEAFYN
                     RGLAKGKLGKFDAAIDDYGEAIRLNPKDAEAPFNRGLAYMRVDKNDFAARDFTEVIVL
                     QPKHADAYVYRGLIRVYQDREKDADADFQKAFQLNPALKKKLEPVIAEAKAKARKAAK
                     "
BASE COUNT         3168 a         3953 c         3751 g         3003 t
ORIGIN      
        1 ggtgtaaccc acatccatct cgccggaaac cagagtagcg accagggtgg ctgccgcgcg
       61 caccaggact attttgggat cgagaccata tttgccaaaa atcccctgct cctgggcgag
      121 ataaagcggt gtcgtgcgcg gactgaccgc ggcataacca atggtaagtt tggtttgcgc
      181 ccctgccggt tccacacgca ctacgattga aaggaaaagg acagcgatcg tggcgaccaa
      241 tcgttgttga tgcgtcatat tcctccacgt actagaacga acgccaaagc gacggcaccg
      301 ctggaacttt atcgctattc aaacaatcgc tgcagctaag cattgaaggc tcgcccctac
      361 ttatcctctg cccgccgctt taccggttcc agacccggcc ggtatgtctt ctcatcgaga
      421 aattcgcgca tgccgcgttc gcgggtatgt tccggatcgg cggcgcgcaa cgccatgctc
      481 ttggcaccga gatactcgta agcttgatcc atatccatgc cgcgcacggc gcggatcgca
      541 tgtttagtgt acgcaaggac catgggactt ttggccatca attcacgggc aatcgtaatc
      601 gtctcctcac gcagtctatc ggccggcacc gccatggtgg cgatgccgat ctccgccgct
      661 ttgcggccgt cgatggtccg gccggtcatg gcataaaaca tggcgttacg aaatccgacc
      721 atctcaccga acactttgct gacattgccg ccgggaatga ttccccagtt cacctcggac
      781 aaaccaaagg tcgcattgtc cgcggcgatg acgatatcgc aggcggcaag cgatacaaat
      841 gcaccgccgt agcaatagcc attgaccatg gcgatagtcg gcttgcgcga ccaggtgagc
      901 agctcagtgg cccagcgccg gtgcgccgtc gcggcgcgaa agcgagccac gggatcttta
      961 tcggtttcgc ggaagtattg tttcaaatcc atccccgcac tccagctgtt ccctgcgccg
     1021 gtcagtacaa tgactttggt ctcttcgtcg gtctccaatc cgtaaaggat ctcttccatt
     1081 tcgacatgga gcgtcgggct catggcattg cgcttctccg gccgattgaa gctaatccag
     1141 gtaattcctt catcgtcggt ctcgacgagt acgcaggtat attctttttt cttagccatt
     1201 ggggttctcc tttattgaat cgctcggaag aaacgcggtt ggtcggcggc tcattgaccc
     1261 tgctcaacaa ccaagtcttg ggcagcaagc cgattcgccc ttgcgtcttg gcgcgcgact
     1321 tcttcaatta cggcggtaag cgaagccggc gcggcaacca gactcggttc gaacagctcc
     1381 gcgaacaccc gataacattt ttcaatcacc tgcgggtgag cacgcaaggt tggcagctga
     1441 cgcccgataa cattgagcgc gaattgcata tcgctgttaa acagccggat actttcgcga
     1501 tagattgccg cgatcgcccc gaccagttgg cgattcccgt cgagaaattg ccgggtggtt
     1561 gccaacggca cccagtggga ttcgagttcg aggctgtcgg cgatgatccg caggcctgcg
     1621 gactcggcca agaaggcttt ctccggcggt aggagcgctg cgtcgagcgt gccgctgagc
     1681 agcgcttcgt aactacgatc gttgagcccg agatcgacga gttcaagatc atcgtctcga
     1741 tcgaggcgcc agcgtctcaa gagcgccagc atcatcatac ccgccatccg gcgcggctgg
     1801 ttgattccca cgcgctttcc tttgaggctc ccgatggact cgatggttga attcgccacc
     1861 aggtaaaatg ccggccgatt caaaacgccg ccgatgatga tcaggtcgtt gccgttgagg
     1921 cgggagaaga tgagcgaagg tgcattgggc gtgccgatcg gcgtcacttg actggcgatg
     1981 tcttcaacga ctttttcttc atcaaattga ttgatgatct cgacggcaat gtcttgcttt
     2041 ttgaacaatc ccgccgcttc gccaacataa ggcaaaaacc gaccgatgct ggaggtgccg
     2101 atgccgattt taatattttc catgcagacc tcaatccaac tgtaacgttg ccggctcatt
     2161 ggccatacgc tcacccgtgc cgacatggcg taagcaaacc cgtaaagtgt cgacctccgc
     2221 catcaccgcc tcatcgggcc gcggtgctca agccgctgca acttcctcgc tcagctatct
     2281 agcgccgaag cgtaattgca atcaaccccg gcgccgcgac cgcggtccta agcggccctc
     2341 ccgcaggcta gacaatagcg gatactcgcg ctattgggtt ggccatgaaa ccagatcaat
     2401 ttatcgacga actttggagc tacgctgccg aggtgccgat ggagcaacat ccctggttcg
     2461 acggcatcgt caagcatcgc tggaccaagg aacaaataat tctcggtgag gtgcagcact
     2521 atctccgagt caggaccaac ccgatttttt ttggctatat ggcgatcaac gcagtttcgg
     2581 ccaaagaata tacggtgatg gagacggtct tggaaaattt catggaggag ttaggcggca
     2641 agcgcaccca tgtcgatatc atgctgcagt ttctcgagga gggcggcatc gggcgcgaac
     2701 aagccgacaa tgcagagccg gcgccaggca cgctggcggc cattgaaatg attatcggct
     2761 gttgccagcg ccgttcagcc ctggaaggcg tcgcgatgct cgcgttcgta gagagccagc
     2821 tcggcggcgc cggtaaaatc tcggagaaag tttatcgcga gctcaccggc cattatggtt
     2881 tttcaccgcg cgcagcggcc acctaccaac ttcacgccgc gcaggacgaa ggccatggca
     2941 gccgccagat cgacgccatc cgcctgcttg ccaccgatga ggcaacacag aacaagattc
     3001 gcgccgcggt gaaattggga ttgaccgcgt tcacgctgga gtgggatggc catgttcagg
     3061 ccatgacggg caagagagag ttctggagcg gggttaaaga tttacaactg cgccaaccga
     3121 ttgtgaattt gtcgagccag aagtcttcag tctagaataa ccgttggttt tcacgtgtcc
     3181 cgaacgggag cactagaggc ttaggatgga caaaaaattc agatcggcca ttcgtccggc
     3241 agatcctttc taagtttttg atattccacg tcgtcgctgt gctgagcacg cagtcgcttc
     3301 agcgccgcga cgacttcttt ctggctctgc ttgacagcgt tggagaccat acccgtggtc
     3361 tcttcgaagc cgaggcctca attgctatgt caaccacgca tccgttggag aatgtagaga
     3421 tcgtcatctc tgctagtcat ggcattttcc tcaattcgag attccggaaa aaccaacttt
     3481 ctgtgtcgtg atcagctcgg tcgcggtatg gggtgcacca gctgcgtcga cgtcaacccg
     3541 caggcgcacg aagatgcgcc aagcttcgcc gcgccactgg cagtgaaaaa taataaaggc
     3601 gttttctgta accagtccgg ccggctcacg acactggatg acactttcga accgttgact
     3661 tgcgcctgct gccacttcca gtgctggagt gaagtgccgc acagccgatt tgaattggcc
     3721 atgggggaag tgcaccgaaa caataaacag cgcaccgtca ccagggttgt ctacttgcca
     3781 gagaatttgc caacatccct ccgccacatc tcgtgccggg gcgatctgct gcaacgcaat
     3841 gcacggctca gatttcgatc tcaccagccc agtctattcg agccgacccg cgctgcgcaa
     3901 ttccttgccg gctggtctaa aaagtgtagc ggcagatttt ggtcaatgac agttgcgaga
     3961 tgattcttaa tctctggatc tccttggcgc ccacgagaaa caaaccatta aactcgccgt
     4021 cggcgacttg gtatgattcg cttcacccgc gcggaccaac ccgaagaggt ctaactcgtc
     4081 ctctggatag tcgggaactg cccttccacg tgcggccact ttttagtttg cgcttgatgt
     4141 ttcgccgctc gtcctcttcc tcgcgaccgg tcataaccgg ggcggcccgg ccgccggcta
     4201 gaatgccccg acgaatatat gccggctcca gttccaatat ttcgcagacg tctttgaatg
     4261 aaaaaggctg aaagtcgtct tccgcgaaaa tccacttctt ggcatcctgg taaagacgaa
     4321 tcgccgaacg cttcgcggat ttctgatact tccaataaca ttcgatggca tcgcttagga
     4381 cggcgagcat taactcgcgc tccgcacccg cgggtccgtt tctagcactc gaaccgaaca
     4441 gggattccgc aatcacggga tcgacgctca cgactttagc aaaatcgctc atgactattc
     4501 ctctcgctat gggcactacg ccccgggcgc gggtgttacg cgcccctatc aagttaaaca
     4561 agccgttgta ttagtcgaat taaacttacg cagcggcaat gcgaggtgga gagcggctgg
     4621 aacgaaggga gacgagcggc gtgcgaacaa aatcctgctg gcgcacgttg cggacgtacg
     4681 tcaggtaaaa ccccggcgtg aacagccggc taccaactgc taaggagagc tgtaagacct
     4741 gcgtcaggcc gctcttaggg gattgttcgt gtttgaaaat ctttgcgctg acagaacgtg
     4801 gaccgtagag aggattacga tcatcgagtt ccggctggcc gatgagctgc aataacacga
     4861 aaaccggtaa gacgcataaa aaagctcgcg caagacgaca acgcgcctgc ccaaactgcg
     4921 cggttttaac gcggaactcc atgcgcgacg aaagatatac gacacaattc agacggtcaa
     4981 ggaccaccag ccgccaatcg tgagcgccga cagttggatc acaaccaccg ccatggttcc
     5041 aatctaagtg cactcgctat gggttgaggc tgagtagttt acgctgcgct tcgtccatgg
     5101 ccgccgtcca gacagtgcgc tccttgtaga actgcacggc gctcgggtga tagggaatgg
     5161 tcacttcggc gtctacggct cgctggcgcg tccagtcttt gaaacttgga tggagcggcg
     5221 gcagtttctc gacgttgtcc cagatgtttt tgaggatcgt ttgcactact cgatcgttgg
     5281 ccgatttacc gctggtgaga tagatatcat aggcgttgac acaggtgtcc tcgacgatgg
     5341 cggtggcacc gccccgcttg atccaatacg gataataacc cggcactgcg gcgcgcagac
     5401 gtttttcacc ctgcgccgaa cagtcgagtg agatatgctt gacgccaatc gcggcgtcag
     5461 cctctttgac cttggcagcg tcgatggccg acatgctgac atcggcgcgc ccctgcatga
     5521 gtgcgtcgac gccttcgttc acgcccggca ccgcgacgat tttcacgtcc tcccatttca
     5581 ttcctgcgcc agctaggaaa ccgaaaatgt tgtaccagtt cgacaactgc gccgggtact
     5641 cccccgtcaa gcgtttgccg cggatatcga gtacgctctt catcggcgca tccttgcgca
     5701 ctagtagtgc gaccatcaac accgccccgc gcatgacgag cctaacattg ggggtgtgct
     5761 gaaacggatt gcgtccgccg atcttcaagc gctcgggtcc ctgataggac agcgccatgt
     5821 ccaccgcgtt gttgatgcca aaatccacct cgccgctatc gagcagcggc agaaacgtgc
     5881 tggtgccgga ataaggctgg acgaccatct gaaatggcgc tccctcgctg gccaccttgg
     5941 aaagcccact ggcgagcgca taaaaaaccg tccccggcgg attcgagccg accgacaccg
     6001 agcgcggcaa cgactgtgcc tcggcccctg cgcaaaaccc gccactgagc agaaacaaaa
     6061 aagaaacaaa ccgagcagac cgatacgaag tcggcgccat agggtttcct cctcggatgc
     6121 gcgactttta tagataggga gcacgcgctg tcaagacaaa agtactgccg gtaaatgaga
     6181 caaaccaccg ctgacgagcc ggggctttat aatatctatg cgcactttaa acgggtgcat
     6241 tcgacaaaac tggttaacga aacaggggtg tcgcggcgcc gcttaccagc attacgtaga
     6301 ttccgaaaag caccagcgtg atcagcagat ttcttttcca atgatcactc aaagcaattc
     6361 cgaatgggag gcccacgata aaaccaatta tatgggcgaa gtaagcgact tgcgggtcgt
     6421 agccgggtat gcggctcttt tcgtagacta cgatgacgtt gtacacgaaa tagataaccg
     6481 cgattaaccc ctgcggcgcg aggaaaagcc aggaaaactt cagcggtcgc acgagcatta
     6541 cgcatccggc caaggcgaag atcgcggccg atgcgccgag cattccggtg gcgcggggca
     6601 aataggcgac gccgagtatg aagcctgtca atccgccggt gaaaaagatc agcagaagct
     6661 ttcgggcacc cacggttttt tccaatgtgt tgccaaagac aaacagaaac agcatgttgc
     6721 cgaacaagtg caagacgttg gcgtggacaa acagcgccac gaacagcgtc cagacttgcc
     6781 ctttgccagg ttgttcagac tgaatattaa attgttttgg gcaaacgccg gctcctgctg
     6841 ccatgcccac aggctggccc cgatgcaagt caggattagc agcgagttga tcttcaaagg
     6901 actaatccaa gaactcgcga ggaagcgata ctgctagtat agcgtctctt aaattaaggc
     6961 gcttattctt cgacgtaaga ttcttcatga tgatcgcttt gtgctcaaac caagtgtttt
     7021 tttatttacc acgagtgatt catgagctta cgctcgccgg tagagcatga aattatcctc
     7081 acccggccta tcccagcgga ctgggcgagg gagttcggat cagagcgtct tgtgttttac
     7141 cctcgcccgc tcgggagagg caagagtgag aacccgtctg cgccgcgagc cgaatttcaa
     7201 aggatcgtgt ccttcgtgtg atactcagct caattcacga tttaaaataa agtgccaaca
     7261 gtgtgagact agcggcttgg cagcgtcgtc accacatgac ccacgatcag gcagcgctct
     7321 aagcttcggc agggccggtg tgcatgtctc cttttgtatc ttccgcttgc ggataggctt
     7381 cgaagctagt gcgcaggctt gttgtcggcc aacggcgaag gatcagaccg atgcctttaa
     7441 ctagtgtcgg cgaatgaact caagcacgtt acggttaaat gcttcgggct gttcccaatt
     7501 gattgaatgc gaagcttcgg gcaggacgat aaactcggcg ccagggattt gggcaagttg
     7561 ccaacgcatc acccagggcg gagcttgcag atcttgatca ccgggcatta atagtgtcgg
     7621 cacgcgaatc gtcgcgagct tggcgaatgt gatcgtctgg cgttgcggtt gcgcagacgc
     7681 gcccttctgt attgaattgt cgtgaatctc caaccagagt ttgagacctt cgggattggt
     7741 cgccatgtag cccatgctaa cctcgcgaaa ctccggcggc caattacgaa aattcggcag
     7801 cgccgtacgt tcgcggattt tgctcaatgc ttcgtcgaca atggcgcccc cactggcagc
     7861 gacgaccaaa ctgcgcagtt gctcgggatg ccagagtaca taatcatagg cggcgaagcc
     7921 accgccggcg acgccgacaa gatggaaccg atcgagtttg agaaattctg tcagcgcgtg
     7981 aaggtcgtcc gcgatggtac cgggctgagg tccggtgtca ggattcgcca cgctccgtcc
     8041 ccagccgcgc cgatcgaacg cgatgactcg ataacccgct tcggcgaagc caggaatatt
     8101 atactgccaa ccgtcggcat tgccggtgtt ggcatgaagc aggatgagcg gaatcccgct
     8161 gccgccgcta tcggtgtacc acaggcgcac gcccgggaga tcggcatagc tcccgttggt
     8221 actagatgag tgtgtcatac cttcggataa acctcctgcg ccatccactc cattcgctcc
     8281 aacgtttccg ccgaggtcgg cctgcgcacg tcgaagatca tcgtgttgac accggcggca
     8341 gcgtactcgc ggatgtcatt ggcgatttct tcggcgcctc ccatgaaccg gcgacggcgg
     8401 tcggcgatct ttttctcttt gtcgaagaga gaacccttga gagcgacgtg gattgtttta
     8461 gggtcgcggc cggctttgac ggcgtgttct gacactgtca ccaggtcttt tgtgatatcc
     8521 gccggctcga gaggaatcgt cggcacaccg ccgatgggat gccagccgtc gcccaactcg
     8581 ccggcgcgct tcagcgcggc tttactgtgg ccgccaatcc aaatcggaat cgtcttttgc
     8641 accggcttgg gcaagaaaat caaatcggag aaattgacaa agcggccttt aaaggtcggt
     8701 cgatcggcag tccataattc acggaacgcg ccgatatatt catcggtcac ccgcccgcgc
     8761 gcggcgtagt cgggcgcctg gaggttttcg aactcttctt tcatccagcc gacgccgaca
     8821 ccgacaatca cccgaccgcc cgaaagcacg tccaagctcg ccaacatctt cgcggtcacg
     8881 ataggattac ggtagggtaa aatcatcaca ctggtggcga aacgaatccg ctgagtcgcg
     8941 ccggcaagaa aactcatggt caccaaagtt tcgagatgaa agccggtgcc ggggtacttg
     9001 ccgtcgacgg tgtacggata aggaacggaa atgttggtcg ggacgatcac gtgatctgcg
     9061 accgtgagca gcgaaaagtt caacgcttcg gcgcgttggg cgaaacgtat caattcttca
     9121 gaggcgacag tcgcactcga cgccggaaga tgtaaaccat actgcatgaa atatttcctc
     9181 ggtggtttgc gacaactgag agagtttgtt ctctcgccaa ggcgccaagc tcggatcaat
     9241 tatcatttcg accgaaggga gaaatctgtt tctgacttcg cgttggcgcc tgtgctcctt
     9301 cgggagattg ccggcttatt cgttctgtaa ccctggacct accccgccgt ggcaactgcc
     9361 gcgctcgccg aagccaacac aactgcatcg ccgatttgaa tttcgccgtc gctcaatact
     9421 gcggctttga gaccactgcg atgctccaag gctctgaaga tcgccggaat cttggtgacc
     9481 gcgactaagt gcccgcaggg ttcgaacagg cggataccgc gcatgcgcac gttgccgatg
     9541 gtgaattcgc ggccaagcaa ttcgatcagc ggcacgcctc gcgtgacaat gttacgccgc
     9601 gtcagaatcc cgatcgcatc gacgccgatc tctttaccgg caagctctaa aacttcatcc
     9661 tcgatcaggc tcacggcttt atccgaacgc ttaacggcgt tccaagattc gcgataaaaa
     9721 cggtcccctt ccagaccacg atcggcaaag gctcgcacgc gcggcacggc aaccatcgcc
     9781 tgcgcggacg cgggcgtaat aaaaatctcc ttgatactgc cctgccatgc ccggtctgct
     9841 ggctgataac cgcgccatag ttcgggcggc atttcttcgg gcatgggccg aatcgcttgc
     9901 gtcatatcac ccccaaaacg acaccggcca ctgatctctc agtggccggt ggttacactt
     9961 gcatagtttg gtcggcgcga tgcttatccg gcctcgtccg catagaaacg cgagtcttcg
    10021 accggcggca cggtcttgtt ctcttcggag gaagcgatta acggcttacc gtccggacgc
    10081 acgcgcatct cctcgccacc ctgggccttg cccttgatgc cggagccgac gcgcagcacg
    10141 accaaattct cggtgccggt gttgagatag cgatagtaag cgccccgggg aatcatcacc
    10201 ccctggtgct tgccgacttt gatctcgccg cctttttcgt cgaacacgct catttggcct
    10261 tcgagaacaa taaacgagtg atcttcgtcg agatgggtgt gaatctcgtt ctcaccgcct
    10321 tcgtagttga tcttggtgtg gatccacatg ttttcggttt ccgccaccaa ctgggtgatg
    10381 cggccctgct tcatgtaggg cgttcgcagt gaaaatttct gcgtgcgcgg tttctccgtc
    10441 ggcgctaatt tttttgctgg ttccatcgtt cgtcctttcc tggggttaat ttaattatta
    10501 atgccgtcga ctttgatgtg gtagagatcg aggacgtttt ggcgcaccac cttgtcgatg
    10561 acgttcttgg gcagatgacc gagctcgtcg gtgatgactt ggcgcgaatg cggccacgtc
    10621 gaggcggcgt gaggatagtc gctcgaccac atgaagttat cttcgccaaa ccagtccatc
    10681 atgcgtccgc cggcgggatc gctaaagaag gtcgcgaaca cctggtcttt aacatactcg
    10741 ccgggcaact ttttcatgta aggcagcggc gccttggggc tgtgacggac atagtacttg
    10801 tcccattcgt gacagtagaa gggaatccag cccacttcat tttcaacgta gaccaatttt
    10861 aatttgggaa atttcgccag cggcccgccg aaaacgaaat cgaataacgt gttggcggca
    10921 tcggtgagtt tctgattgac ggtcatgcga tacgtatcca aacccttgcg gtcgaaccgg
    10981 ctgtagttga agccggtcag gatgtggaca ttaaccggca tgtcgagctc gccggccgct
    11041 tcccagaacg ggttgtaata atcgctggtg aaaggcagat gatccggcgg cacctgccag
    11101 atcatgcagc cgatcatgcc attcttcttg caacgttcga gttctttgat cgcgttgggg
    11161 atgttgtaca tcgagatcat gggaataccg aacaagcggt tgggcgccac cttacaatac
    11221 tccatcatcc agtcgttggc gatttcaaag caggcctctt gaagcggcgc gtcttccatc
    11281 gcgtagaggc gcaatccata ggtggcgtaa agcacttcgg cgctgacgcc gtccaccgcc
    11341 atctcatcca aacgcgcctt agggtcatgt ccgccgggct tttcgccggc ggtgttgcgc
    11401 ggcggaaact tcgggaattt ggcggcaaga ctcggcggaa gatttttcag ccagagatct
    11461 tctggttcca tgatatgcga atcggacgag acgattttat cttggccgag cacctgttcg
    11521 gctgtgcgct tgacgcctgc tgcggtagtc atgagtttcc tcctatggtt ttaggactta
    11581 ttttcttttg atttatgcct ttttgggcca tttgtctagg gcaatcaggc cgtcgccaga
    11641 ttggacatta gtcgaaccat acgttacacc tctgaacgat gataagagct atcaagcgtt
    11701 atctctgcaa ttgcttgtcg atcctgctga tccctgcttc gctcttcgcc gccgaagcac
    11761 aaaatgcttt caacgaagga gtaaaacttt acaaatccgc gaaatataaa gaggcggttg
    11821 ccgccttcaa ccgagcgatc aaggccgcgc cgaaatcgga cgaggcttac aacaaccgcg
    11881 gcctggctta tgcgaagttg ggccaacaag acaacgcgct caaagactac gatgaagcgc
    11941 tcaagttaaa tcccactctc accgatgctc agttcaaccg cggcaatatt tttttcactc
    12001 aaaagcagta cgagcgggca gtaaaagact tcgacgaagt gatcaaacag gatcccaaag
    12061 cgccgcgggt tttcgtccag cgtggcttgg cattgttaaa tttgaaacag ttcgataagg
    12121 cggtttcgga cttcgaccag gcccagaagc tcgatcccaa agatattgaa atttattcca
    12181 atcgcggctt ggctcatcgc cgtctcggca aaaatgattt agcgttgctg gactacagcg
    12241 aagcgatcaa gctcgaccca aaaaacggcg agaactataa taaccgcgcc aatgcctact
    12301 cgcttttggg caagtacgac gaagcgctga aagattacga cgaagcggtc aagctgaacg
    12361 ccaaggacgc gcgcatctac gccaatcgcg gcgtcatcaa gcaacgcttg aaaagagatg
    12421 acgaagccca gaaagacttt caaaaagccc tggaactgga cgccagctta aaggacaagc
    12481 tagcgccttt cctcggccca cttaaaggta agtaaattat gctgcgtgtt gcagatgttt
    12541 ggcaattgac gcttcgatca ccaaggttac ctttcatcgg cagggcgcag cgttccatcc
    12601 tcgcgctcct cctgtcttgc gcgttgaaat ctttagtccc aatcaatgcc atcgcgctgg
    12661 taactctcgc aggaatcatc ggccccctga acggagcggc gccagcgatg gccggcctgg
    12721 aagaaccgct caagaccgcg ctcgagttgc atgagagcgg caagatcaaa gaagcgattg
    12781 ccgaatacga caaagtcata aaagccaatc cgaaaatggc cgaagcttac ttcaatcgcg
    12841 gcaatgccta tttcgacttg ggacaaaacc aacaagcgct aaaagactac agcgaagcga
    12901 ttcggctcaa ccccaaagac gccgaggctt atttcaaccg cggcaacacc tatcggcgtt
    12961 tgaaacaaga cccggcagcg ctcaacgatt ttaccaatgc cattaagctc aatcccgacg
    13021 atgccaagag ctataccaat cgcgccagca tccacttcga cgcgcggcgt tatgaagcgg
    13081 cgcttaagga ccaaaacgaa gcgctgcgga tcgatccgaa ggacgcgcag cattacttta
    13141 accgaggcaa cagttatttg gcgctcgata aaaaagacga cgcaatcaaa gactacgacg
    13201 ccgctctcag ccgcgacccc aagctcgccg atgcctatta caaccgcggc atcgtctatg
    13261 ccgatgcatt gaagtacgac aatgccatcc gcgatttcga cgaagcgatc aagctcaatc
    13321 caaagaatcc cgaagccttt tacaaccgcg gactcgccaa ggggaaactc ggtaagttcg
    13381 acgccgccat cgacgactac ggcgaagcga tccgtctcaa tcccaaggac gccgaggcgc
    13441 ccttcaaccg cggcctagct tacatgcgcg tcgacaagaa tgatttcgcc gcccgggact
    13501 tcaccgaagt gatcgtgctg caaccgaaac atgccgacgc ctacgtttac cgcggcctga
    13561 tccgcgtcta tcaggaccgc gagaaagacg ccgacgccga tttccaaaaa gcctttcagt
    13621 tgaatccggc gctaaagaaa aaattggagc cggtcatcgc cgaggcgaaa gccaaggcga
    13681 gaaaagccgc gaagtagact gcctctctgc aaatggcgcc gcggcggtgc atgaagccga
    13741 caacggctac gccgaggtca gcagtggcat ccaactgccg agtaaatcga tgatggtaag
    13801 tccgtaatgc cggtgaaaac cggcatccat cttcgttttg actcgccagt cagaaactgg
    13861 attccggcgt gcgcc
//
LOCUS       DATLCH010000082         9152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10567971, whole genome shotgun sequence.
ACCESSION   DATLCH010000082 DATLCH010000000
VERSION     DATLCH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10567971"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VMZ02_02420"
     CDS             <1..706
                     /locus_tag="VMZ02_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR70811.1"
                     /translation="YVEAPVYAAFVEKVVSRVNGIRQGQSADEVDIGCMTSEAQLNKV
                     AAQVDEARAKGARNLTGGRRNPSLPGYYYEPTVLVDVDHSMAVMSEETFGPVIPIMQV
                     KDPQEAVRLANDSRYGLSASIFSRDKAAATSLAEELNSGAVCVNDNLINFIIPEAPMG
                     GRKDSGLGHRHGAVGIHKYCQQKTIVIDRFGLKEEFPWYPASKKKADQTRHLLNLLCH
                     SGWGHKLRALRGLMRS"
     gene            786..2168
                     /locus_tag="VMZ02_02425"
     CDS             786..2168
                     /locus_tag="VMZ02_02425"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR70812.1"
                     /translation="MAKDLPSFIAKEGATREGAVIREKNFVDANNYETVAYLKHLDIR
                     NEPKMVLFENLPALNGKKSEFPMFYNPWVSRQYIADSLDMGDIKSPMEVSLEVAKLEQ
                     QQGRIEVIASEKAPVKEVVLTGDKADLRVLPMPMHQKGDSGPYHTMTCAMKGLDQEYY
                     DITFTKNKYHDPKHMSFSAHKHHHLEAMACEYEEKNLRAPVIVILGHHPAFYLSSCAM
                     TPMGNNDYLTASAFLKEPLRLTPSTTWGDKFMVPADAEVIIEGEILPGVRKEQNPFGE
                     ILGYAQPKMDVPVIEVTAITHRRGGIVEDFWPGHMDHWNLGSVPKEGSTYNVIKKNVP
                     GIKGIHLAASGCGRCICYISIKKEFENEPNKAGMQAFVEMPNLKLAVIVDDDIDVFNE
                     REVMWAVATRVHWDKDIEIIREVQSFRGWLGDTVAIIDATIPLKSKAEWPVRNEVEPA
                     AFERVAKFFK"
     gene            2310..2606
                     /locus_tag="VMZ02_02430"
                     /pseudo
     CDS             2310..2606
                     /locus_tag="VMZ02_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(2615..3769)
                     /locus_tag="VMZ02_02435"
     CDS             complement(2615..3769)
                     /locus_tag="VMZ02_02435"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HUR70813.1"
                     /translation="MNHRVDPILPGAAIGVLGSGQLGRMFAIAARQMGYRVHTFSPET
                     DTPTGQVADIEIAASYDDLDAVRRFAQAVQVVTFEFENVPAACVEAAADCVPVRPGGS
                     ALHITQNRLREKNFLASNGFPTTKFAAVQTVDELKPALDQVGFPAIMKTASSGYDGKG
                     QAKVANIDDAVKAFHFCGGHQMIAEGFVDFELEFSVVVARGLDGQFAHWGSIQNRHRH
                     HILDLSFAPARMSESVNARAVEIARALADKLDLVGVLCIEFFLGRDGEILVNELAPRP
                     HNSGHLTINASHTSQFEQQLRAVCGLPLGSVEFVAAAAMANLLGDLWQRGEPNWPAAC
                     ALPEVKLHLYGKIEPRPGRKMGHLTALAATAELAVETVLQARQKLQRVDD"
     gene            complement(3766..4260)
                     /gene="purE"
                     /locus_tag="VMZ02_02440"
     CDS             complement(3766..4260)
                     /gene="purE"
                     /locus_tag="VMZ02_02440"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HUR70814.1"
                     /translation="MPPAPAALVSIIMGSQSDWQTMQHASTTLKEFAVDHECLIVSAH
                     RTPAWLAEFANGAEGRGIEVIIAGAGGAAHLPGMTAAQTLIPVLGVPVESSALKGMDS
                     LLSIVQMPGGIPVGTMAIGNAGATNAALLAIAILANKRPELREKLRQFRANQTTAVRE
                     HKLP"
     gene            4694..7243
                     /locus_tag="VMZ02_02445"
     CDS             4694..7243
                     /locus_tag="VMZ02_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HUR70815.1"
                     /translation="MAGLIIGLFSRRLGPKVCHIGVAAEVIAFIGALAVLYDVTTTAP
                     RTIHLSPSGQGFWQISLHLDRLAAVMLVHIAAISGLIHLFSLRYMQQERGYARFHSLL
                     AFTTFVLFGMVSSANLLLLFIFWQLLSWLVPLLSFNYEHTPTLRGAFRTFIMQRAGDV
                     AFLGGIFVAARVYETLELAELFTRATAVSSTLILWPGGMEIHATSAITMLIFISAMSK
                     SAQFPFHMWLPDSLYAPTPVTAFLHAGIINAGGFLLARLAPLFALSPITLHWVFAAGM
                     LTALLGSSMMLVQNDIKKTLGYSTIGQMGFMIMECGLGAYGLAIFHLIAHGLFKGSIF
                     LNCGYVIHAARQDPRLPRRHTPVEAPEFSSLAWVTGFVTTLILPLIIVLGAHGVLNIA
                     LGDSQGTIIFLFFGWATSSQAILTLYRLRAVASWKVAVSMLSALFIVVVTYLFAAESF
                     THFLFPAPGEVARHFGAGALPAALFDLLVTAFALVIVAGWILIYAASHGRTISVPAWT
                     DKLQVAVYLLLINRLYLDTFAIKLQTRCTTLLDGLNRSRSFPYIAGLLAVALALVNWA
                     PSLTMTPRQVLQLVAVTLMLPLFPMHVFYVAALSRTSGYLAATLAVLLPAAGSLGLAD
                     LAQELPRDILRMMNVLALVGAVYGSIKALAQVRMPDLVAYASVAFFSVLWWYFTASGT
                     FNVAVFVYITAVALLTAAQLIAWQLLRKRYGNLTLNLFHGLARPMPRFATLFMLCVMA
                     AVVLPPFALFSGPLKMSVLTSGALSFGLIVALFTWFVASWYLFRMMQRLLFGHHRAEI
                     RYLDLHTTEVVGLLILLSISALLVAMPSAWINMEPVDHSQRATMELLLWHK"
     gene            7231..>9152
                     /locus_tag="VMZ02_02450"
     CDS             7231..>9152
                     /locus_tag="VMZ02_02450"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HUR70816.1"
                     /translation="MAQVTSKLYSESQRMQLRALIRLASDVVSYYWPMRTFVHHNPLH
                     GLEDLRFEEAVQIAHKTLGGQGFLSNAEFRDYVRVGRILPRHLDVILQPMAQLDQVRL
                     GIREISHLDVLRACLLGDTAAPPSDSFDSQLARHPDRNTINNLAGRLTPNLAQAPMRD
                     VNSGHHDLGSNETLASCCDRLLGSQITETINREMVKWCAAFLDEGHAPWPMPGREHGF
                     YSAWKFLAQREWAPGGIESSRKKLAALPLEADDSLLQSLTVLGVDANAWQNYLALHLA
                     ALSGWAGFIKWRTDQREYEWQTSYPIDLVQYLAVRLWYERELVEQVCRQQLGISGNLE
                     ALSAQLVKSPERSSERDDKEKAIHDDARRHAWRLVLLARSLELEPQLLTQAPDNTLRT
                     LLDWINAFPETVHGPIWLNAFELGYQEQLLEKLRFKIGRLNDLPARWPVVQDSVEIHP
                     VETEAKVRPQAQAVFCIDVRSEPLRRNLEATGDYETFGFAGFFAVAIRYQALGSDHET
                     DQFPVILKTKNLVREIPRSYHGQFLFRHRNREKLFEAFHTLVYDLKENVVTPYVMVES
                     LGWFYSLPMIGKNLFATNYRNAVEWLRRKFLPPVATTLTVDKLSRQEVEEMLAAEQRA
                     TTRRALQDKFGERNLNL"
BASE COUNT         2034 a         2582 c         2440 g         2096 t
ORIGIN      
        1 ttacgtcgaa gcgcccgtct acgctgcctt tgtcgaaaaa gttgtcagcc gagttaatgg
       61 cattcgccag ggccaaagcg ccgacgaggt cgacatcggc tgcatgactt cggaagcaca
      121 actgaacaaa gtcgccgcac aggtcgatga agcccgtgcc aaaggtgcaa gaaatctcac
      181 gggcggacgg cgcaatccga gtttgcccgg ctactactac gaaccgacgg tactcgtcga
      241 tgtcgatcac tcgatggcag tcatgtccga agaaactttc gggccggtca ttcccatcat
      301 gcaagttaag gatccccagg aagcagtcag actggccaac gattcgcgct acggactgag
      361 tgcgagcatc ttctcgcggg acaaagcggc ggcaacaagt cttgccgaag aactcaacag
      421 cggcgcggtt tgcgtcaacg acaatttgat caacttcatc attcccgaag cgcccatggg
      481 cggacgcaaa gacagcggct tgggccatcg ccatggcgcc gtcggcattc ataagtactg
      541 tcaacaaaaa accattgtca tcgatcgctt tggtctcaaa gaagagttcc catggtatcc
      601 agcgagcaag aaaaaagccg accagacgcg tcacctgctc aacctgcttt gccactcggg
      661 ttggggccac aaattacgcg ctctcagagg gcttatgcgc agttaggcag cttgcactcc
      721 caccacgatt catctaatta gaaacgatac gctagcgagg taaataaatt tacaggagaa
      781 ggtccatggc caaagatctg ccaagtttta ttgccaaaga gggcgccacg cgcgaaggcg
      841 cagtgattcg cgagaaaaat ttcgttgatg cgaacaatta cgaaactgtc gcttacttga
      901 agcatttgga tatccgcaac gaaccaaaaa tggtcttgtt cgaaaacctg cctgctttga
      961 acggcaaaaa atccgaattc ccgatgttct acaatccctg ggtgtcgcgt caatatatcg
     1021 ccgactcgct ggacatgggt gatattaagt cgccgatgga agtcagcttg gaagttgcca
     1081 aactcgaaca gcaacagggt aggatcgagg tgatcgcgtc ggaaaaagct ccggtaaagg
     1141 aagtcgtgtt gaccggcgac aaggcggact taagagtgct gccaatgcct atgcatcaga
     1201 aaggcgattc cggcccgtat cacacgatga cctgcgccat gaagggcttg gatcaggaat
     1261 actacgacat aacgttcacc aaaaataagt atcacgaccc caagcatatg agcttctcgg
     1321 cgcacaaaca tcatcatttg gaagcgatgg cttgcgaata tgaggagaaa aatctgcgcg
     1381 cgccggtcat cgttattctc ggccaccatc cggcatttta tctctcctcc tgcgccatga
     1441 cccccatggg caataacgat tatctgaccg cttcggcatt tttgaaggag cccttgcgcc
     1501 tgacgccatc gaccacctgg ggcgataagt ttatggttcc cgcggatgcc gaagtcatca
     1561 tcgaaggtga gatcttgccg ggcgtgcgca aggagcagaa tcctttcggt gaaattctcg
     1621 gctacgcgca accgaaaatg gacgtgccag tgatcgaagt caccgccatc acccaccggc
     1681 gtggcggcat cgtcgaggac ttctggcccg gccacatgga ccattggaac cttggctccg
     1741 tgcccaagga aggcagcacc tacaacgtta tcaagaaaaa cgttcctggg attaagggta
     1801 ttcatctggc cgcatcgggc tgcggccgtt gcatctgcta catttcgatc aaaaaagaat
     1861 tcgagaacga acccaacaag gccggcatgc aggccttcgt cgagatgccc aatttaaaac
     1921 tagcggtgat cgtcgacgat gatatcgacg ttttcaacga acgtgaagtg atgtgggccg
     1981 tggccactcg ggttcattgg gacaaagaca tcgagatcat ccgcgaagtc caaagcttcc
     2041 gcggatggtt gggcgatacg gtagcgatta tcgatgcgac gattcctctg aagtcgaagg
     2101 ccgaatggcc ggtacgcaac gaggttgaac cagccgcctt tgagcgggtc gcaaagttct
     2161 ttaaataggc tcgggaaaaa tgaagtagaa gtactggagt agacggagtt atgggttacc
     2221 gcccagcact cctctactcc agcactccag agatagccat ggccgacaaa ctccgattca
     2281 tctacaaagc tacgtgaacg acttgccgca aagcaagaag tttcttgcaa gcaaaaaaag
     2341 tcgattttga ggagcgagac tacggcaaga cgccgttcac cgagaaggaa ctgcgcgaac
     2401 tgatcggcag cgatccgatc gaggcgttct tgaacacgcg cacgccgctt tatcgcgaga
     2461 aaaacatgaa gcaaaaaccg cccagcaaag atgaggcgat taaactcatg ttgaaagatc
     2521 ccaacctgtt aaagcggccg gtcattataa aaggcaagaa gaaattaacc ggcttcaatg
     2581 aagcggaagt aaaggcactg ctctagagac ttcgctagtc gtcaacccgt tgtaacttct
     2641 gccgagcctg tagcacagtc tccacggcaa gctcggccgt cgcagcaagt gctgtcagat
     2701 gtcccatttt acgaccgggg cgaggctcga tcttaccgta aagatgaagt ttgacctcgg
     2761 gcagtgcaca agctgccggc cagttgggtt ctcctctctg ccatagatct cccaacaaat
     2821 tagccatcgc tgctgcagcg acgaactcca ccgagccgag cggtaaaccg cacacggcgc
     2881 gcaactgctg ctcgaattga ctggtgtgcg aagcgttaat cgtcaagtgt cccgagttgt
     2941 gcggccgggg ggcgagttcg ttgaccagaa tctcaccgtc gcgaccgaga aaaaattcga
     3001 tacacagaac gccgaccaga tcgagtttat cggccagggc cctggcgatt tcgacggccc
     3061 tggcattaac cgattcgctc atgcgcgccg gtgcaaatga taaatcgaga atgtgatgtc
     3121 tatgacgatt ttgaatcgat ccccagtggg caaactggcc gtctaggccg cgtgccacga
     3181 ccacggagaa ttccagttca aagtcgacaa acccttcggc gatcatttgg tggccgccgc
     3241 agaagtgaaa cgccttcacc gcatcgtcaa tattagcgac ctttgcctgc cctttgccgt
     3301 cgtaaccgga actggcagtt ttcatgattg ccgggaaccc gacctgatcc agtgccggtt
     3361 tgagctcgtc aacggtttgt acggcggcga acttcgtcgt tggaaagccg ttacttgcaa
     3421 gaaagttttt ttcgcgcagg cggttttgcg tgatatgcag agccgagccg ccggggcgaa
     3481 caggtacgca atccgcggcc gcctcaacac aggcagcggg aacgttttcg aattcaaaag
     3541 tgaccacttg aacggcttgg gcaaaacgcc gcacggcgtc aagatcgtca tacgacgcgg
     3601 cgatttcaat atcggcaacc tgccctgtcg gtgtgtcggt ctcaggggaa aacgtatgga
     3661 cgcggtagcc catttgacgc gcggcgatcg cgaacatgcg acccaactgg ccgctgccaa
     3721 gcacgccgat ggcggcgccg ggcaggatgg gatcgacgcg gtgattcacg gtaacttatg
     3781 ctcgcgcacg gctgtggtct gattggcgcg gaactggcgc agtttctcgc gtagttcagg
     3841 ccgtttgttg gctaggattg caatcgccag caatgccgcg ttcgtcgcgc ccgcgttgcc
     3901 gatcgccatg gtaccgacag gtatcccgcc aggcatctgg acaatcgaca ataacgaatc
     3961 catgcctttg agcgcactac tctcgacggg cactccgagt acgggaatca atgtttgcgc
     4021 cgcggtcatg ccgggaagat gtgccgcgcc gccggcgccg gcaatgatca cctcgatgcc
     4081 acgcccctca gcgccgttag cgaactcggc cagccatgcc ggcgtgcgat gcgccgatac
     4141 gataagacat tcatgatcga cggcaaattc tttgagggtt gtcgatgcat gctgcatggt
     4201 ctgccagtcg gattggctgc ccatgatgat gctaactaga gctgcaggtg ctggtggcat
     4261 gtgggctaat tccttactac gttagaatta acaacaagct taaaccctgt tacagagcaa
     4321 agcataagtc aatccgcaat agctcagtcc tggcttgacg aaagcccggc atctacgtcg
     4381 aaccgctacc cggcggcttt tcgagcggac tgacttaaga atcggttgcg ttatcaaaat
     4441 gacgctgcta tttgttcgag agtctgcggc tgggacgatg agattttcca accaatcgac
     4501 ccgctaaata aaaaattaac ttgtcaggtc ttcggcggcg acgtgctcac gcgccgtctc
     4561 ggcacccagt cggtagggaa acccgggtga tgcttgactt agcgagcgac agataatccg
     4621 cagccgcgga gcgacgttcc gttttcaaat ggtcttctcg tatttacctg cgatcatcct
     4681 gctggcgcca ttgatggccg gtttgatcat cggtcttttc tcgcgccgcc tcggtccgaa
     4741 ggtttgtcat atcggtgtcg cggccgaggt aatcgccttt attggcgcgc tggcagtgct
     4801 gtacgacgtc accactaccg cgccccgaac cattcattta tcgccctccg gccagggctt
     4861 ttggcaaata agtttgcacc tcgaccggct cgcagcggtg atgctggtgc atatcgcggc
     4921 aatcagcggc ctgatccatc ttttttccct gcgctacatg caacaggagc ggggttatgc
     4981 ccgctttcat agcctattgg ctttcaccac tttcgtattg ttcggcatgg tctcgagtgc
     5041 caacttgctc ctgctcttta ttttttggca gttgctcagt tggctggtgc cgttgctgtc
     5101 cttcaactac gaacacactc ccaccctgcg cggcgctttt agaactttta tcatgcagcg
     5161 cgccggtgac gttgcctttc tcggcggcat ctttgtcgct gctcgggttt acgagactct
     5221 cgaattggca gagcttttta cccgcgccac tgcggtgtca agcaccctaa tcctatggcc
     5281 cggtgggatg gaaattcacg ccactagcgc gataaccatg ctcatcttca tcagcgccat
     5341 gagcaagtcg gcacagtttc ccttccacat gtggctgccc gattccctgt acgcacccac
     5401 ccctgtgacg gcctttctac atgcgggaat tatcaacgcg ggtggcttcc tgttggcaag
     5461 attggcgccg ctgttcgccc tcagtccgat aacgctgcat tgggtttttg ccgccggcat
     5521 gcttaccgcc ctcctcggat cgtcgatgat gttggtgcag aacgacatca aaaaaacctt
     5581 aggctattcc actattggcc aaatgggttt tatgattatg gagtgcgggc taggagccta
     5641 tggcttggcg atttttcatt tgatcgctca tggactgttt aaggggtcga tttttttgaa
     5701 ctgcggctat gtgattcatg cagcccggca ggacccgaga cttccccgcc gccatacgcc
     5761 ggtggaggcc cccgagtttt caagtctggc ctgggtcacg ggcttcgtca cgacactcat
     5821 ccttccgtta atcatcgtgc ttggggcgca cggcgtcctg aacattgcgt tgggagattc
     5881 acaaggcacg atcatctttc tcttcttcgg ttgggccacg tcgtcacagg cgattctgac
     5941 gctgtaccgc ctgcgggcgg ttgcctcttg gaaggtcgcg gtgagcatgc tctcagccct
     6001 gttcatcgtc gttgtaacct accttttcgc tgccgaaagt ttcacccact tcctgtttcc
     6061 agcgccgggc gaagtggcgc gccactttgg cgccggcgcc ttgccggccg cgctgttcga
     6121 tctattggta accgcattcg ctctggtcat cgtcgccgga tggatactga tttatgctgc
     6181 cagccatggt cgcaccatct cggtccccgc gtggacagac aagttacagg tcgctgttta
     6241 cttgctgctc attaaccggc tgtatctcga caccttcgca atcaagctgc aaacgcgctg
     6301 tacgacgctg ctcgacggct tgaaccgcag ccggtcattt ccttacatcg caggtttgtt
     6361 agctgtcgct ctggctttgg tgaattgggc gccgtcgttg accatgactc cgcgacaagt
     6421 cctgcaactg gtcgccgtga cactgatgct tccgctattc ccgatgcatg tattctatgt
     6481 cgcggcactg agccgaacat ctggatatct cgcagcaact cttgcggtac tgctgccggc
     6541 tgcgggctct ttgggtctcg ccgatttggc tcaagaactt cctcgcgaca tccttcgaat
     6601 gatgaatgta ctagcccttg tcggcgcggt atacggatcg atcaaggctc ttgcccaggt
     6661 gcgcatgccg gatctcgtgg cctatgccag cgtcgcgttc ttctcggtgc tttggtggta
     6721 tttcacggcg tcggggactt ttaacgtcgc ggtcttcgtt tatataaccg ccgtggcact
     6781 gctaacggcc gcgcagctca ttgcctggca actgctgcgc aagcgttatg gcaatctcac
     6841 actaaatcta ttccacggcc tggcgcgacc tatgccacgg tttgccaccc tgttcatgtt
     6901 gtgcgtcatg gcggccgtgg tcctaccgcc gtttgcacta ttttccggcc ctctaaaaat
     6961 gtcagtgctt acatcagggg cgctttcttt tggcttgatc gtcgctttgt tcacctggtt
     7021 tgtggcttca tggtatttgt tccgtatgat gcagcgactt ttattcggcc accaccgtgc
     7081 cgagatccgt tatctcgatc tgcacaccac tgaggttgtc ggtctcctaa tcctgctatc
     7141 aatttcagcc ctgctcgtcg caatgccgag cgcttggata aatatggaac cggtcgatca
     7201 cagccagcgc gccaccatgg agttgttgct gtggcacaag taaccagcaa actttacagc
     7261 gagagtcagc ggatgcagct cagagccttg atccgcctgg ccagcgatgt tgtctcttac
     7321 tactggccca tgcgcacatt cgtgcaccac aacccattgc atggccttga ggatttacgt
     7381 ttcgaggaag ccgtccagat tgctcacaag accctgggcg gccaaggctt tttgtccaat
     7441 gccgagtttc gcgactacgt tcgcgtcgga cgaatcctgc cacgccatct cgatgtcatc
     7501 ttgcaaccaa tggcacagct cgatcaggta agactcggaa ttcgcgaaat tagtcatttg
     7561 gatgtgctgc gcgcgtgcct gctcggcgac accgcggctc cgccgtcgga cagcttcgat
     7621 agccaattgg cccgacatcc agatcgcaac accattaaca atttggccgg ccgcctcacg
     7681 cccaatctcg ctcaggctcc aatgcgcgac gttaatagcg gacaccacga cctcggtagc
     7741 aatgagacgt tagcgagctg ctgcgatcgc cttctcggca gccaaatcac tgaaacaatc
     7801 aaccgagaaa tggtcaaatg gtgcgcagcc ttcctcgacg aaggccatgc tccgtggccg
     7861 atgccaggcc gggaacacgg attttactcc gcatggaagt ttctcgctca acgcgaatgg
     7921 gcccctggtg gtatcgagag cagccgaaag aaactggccg ctctgccgct agaagcagac
     7981 gattcactgc tccaaagttt aaccgtactg ggcgtcgatg cgaatgcatg gcaaaattat
     8041 ttggcgctcc atctcgccgc gttgtctggc tgggctggtt tcattaagtg gcgcacagat
     8101 caaagggaat acgagtggca gacctcctac ccgatcgatt tagttcagta tctcgcggtg
     8161 cgtctctggt acgagcgcga actagtagag caagtctgcc gacagcaact tggcataagt
     8221 ggaaacctcg aagccctctc cgcccaactg gtaaaatccc ccgaacgatc gagtgaacgc
     8281 gacgacaagg agaaagcgat ccacgacgac gctcgtcggc atgcatggcg cctcgtcctg
     8341 cttgcacgat cgctggaatt ggaaccgcag ctgctaaccc aggcgccgga taacacctta
     8401 cgcacgcttc tcgactggat caacgcattt cccgaaacag tacatggacc aatttggctc
     8461 aacgccttcg aactcggcta tcaagaacag ctgctggaaa aactccgatt caagatcgga
     8521 cggttgaacg atctaccggc acgttggccg gtcgttcagg actccgttga gattcaccct
     8581 gtcgaaactg aagcgaaagt acgcccccag gcccaggcgg ttttttgcat cgacgtgcgc
     8641 tcggaaccgt tgcggcgtaa tctcgaagcc accggcgatt acgagacctt tggcttcgcc
     8701 ggattttttg ctgttgctat ccgctatcag gctctcggca gcgaccatga aaccgaccag
     8761 ttccctgtca tactcaagac taagaatctg gttcgtgaaa ttccccgcag ttaccacgga
     8821 caatttctct ttagacatcg caatcgggaa aaacttttcg aagcgtttca cacattggtt
     8881 tacgatctca aagagaatgt cgtgacaccg tatgtcatgg tcgagtccct cggttggttt
     8941 tacagtctgc cgatgatcgg caaaaatctt ttcgccacca attatcgcaa tgctgtagag
     9001 tggcttcggc gtaagtttct gccgcccgtc gcgacaacct tgacggtcga taaactgtcg
     9061 cgccaggaag ttgaagagat gcttgccgcc gaacagcgcg cgaccacgcg ccgggcgtta
     9121 caagataaat ttggcgagcg caatttgaat ct
//
LOCUS       DATLCH010000083         3745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9206224, whole genome shotgun sequence.
ACCESSION   DATLCH010000083 DATLCH010000000
VERSION     DATLCH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9206224"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VMZ02_02455"
     CDS             complement(<1..971)
                     /locus_tag="VMZ02_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70817.1"
                     /translation="MRDSRKWLFTIALYWGLGAAAALAAQAEAWKADWDKTLEAAKKE
                     GQLTLYGSPEYEGLFGELTKKYPEIKITGVFNRGADAARRLMSERRAEKYTGDLYLNG
                     MTTGYNVFYKAKVLDPIPPQLILPEVTDATKWWRGKHQYVDPENHYLLNFNGENRMVV
                     AFNTKLVNPVEIKSYWDLLNPKWKGKIVAYDPTQGGSGDATRFFYHHKGLGREFIKRI
                     LTETDIVISRDTRQMGDWLAGGKYAISIFGPVSRMDLDLMQTQGLPVDWFKPDQLKEG
                     TYITGGSGGVALINKAPHPNAAKIGLNWLLSREGQIAYQRLFTQGNDG"
     gene            complement(1038..2171)
                     /locus_tag="VMZ02_02460"
     CDS             complement(1038..2171)
                     /locus_tag="VMZ02_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70818.1"
                     /translation="MISKTQVVSWFVIVFVTVIGQTNGLAAAADAWSVEWEKTVTAAK
                     KEGEVSFYGSEGYEKIFEVFQKKYPEIKVKSNTNRRGNEHGQAVMTERRAGQYLVDLF
                     INGVVTPNTVFYKANILEPIRPQLILPEVVDESKWWSGKHLYADPEAKYIFVFQGNVH
                     GSENAYNTKLVNPKDIKSYWDFLDPKWKGKMVAYDPNRVSTVAHSLRFLYNHPDLGAE
                     YVRRLFSEMELTYSRDERQMIDWLGAGKYAIAFFVTEVEAAAKQGLPIRDFEPSQFKE
                     GAFVGPSQGGVNLFKNAPHPNAAKIAINWLLSREGQEAYQKVFASVGDIRQSMREDVS
                     TDVIPASQRRQKGVRYIYAGRAEWLDMAPVSKVIKEAQAAKKN"
     gene            complement(2203..3639)
                     /locus_tag="VMZ02_02465"
     CDS             complement(2203..3639)
                     /locus_tag="VMZ02_02465"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR70819.1"
                     /translation="MGNETSLVYKDLRGYLDAADKLGELRHVRGADWDLELGAITEVA
                     ARANNPKVVLFDEIKGYPKGFRVVTNPVCSAATTALAFGLDPKWNGLDIIRQWKERLG
                     KQTPLKPVEVSTGPITENCDSGDKVDLLKFPTPRWHEEDGGRYIGTGCMVIMEDPDGK
                     WTNVGTYRVCVHDKNTLGIWISPGKHGRLIREKYWAQGKSCPVVISFGQDPILAKVAS
                     WYEAWGVSELEIAGWLFGEPVKYIRGKETGLPIPATSELAIEGFIVPPQEDSRSEGPF
                     GEWTGYYASGTRNEPVVKVKNVFYRNDPIIFGMPPGISGKSVPLSAANVWLALEKAGV
                     TDVQGVWEYQTRYITAISIKQKYGGHAKRTAMAMLGSTYGYHRRFVIIVDDDVDPSNI
                     DDVLWALATRCDPATAINVITDAWSTPLDPRITPDARAARNFTNSVAIINACQPYHWK
                     DQYAKTCYFPEETRRAAAAKWKGVLKLD"
BASE COUNT          769 a         1119 c          956 g          901 t
ORIGIN      
        1 ccatcattgc cctgggtaaa caaacgttga taagcgatct gcccttcgcg cgacaacagc
       61 cagttcaaac cgatcttggc ggcgttggga tggggcgctt tgttgatcaa tgcgacgccg
      121 cccgagccgc cggtaatata cgtgccttcc ttcaactgat cgggcttgaa ccaatcgacc
      181 ggcagacctt gagtctgcat caaatccaga tccatccgcg acaccgggcc aaaaatcgag
      241 atcgcatact tccctcccgc cagccagtcg cccatttggc gcgtatctct gctgatgaca
      301 atatcggtct cggtgagaat ccgcttgata aactcgcggc ccaacccttt atggtggtag
      361 aaaaaccgtg tcgcatcgcc ggagccgcct tgggtcggat cgtaggcgac gatttttcct
      421 ttccacttgg ggttgagcaa atcccaatag gatttaattt ccacggggtt caccagcttg
      481 gtattgaacg ccaccaccat gcggttttcg ccgttgaagt tgagcaggta gtgattttcc
      541 ggatcgacgt attggtgttt gccgcgccac cacttggtgg catcggtaac ttccggaaga
      601 atcagctgcg gcgggatcgg atcgagaacc ttggctttgt aaaacacgtt gtagccggtc
      661 gtcatgccgt tgagatagag atcgccggta tatttttcgg cgcgccgctc cgacatcagc
      721 cggcgcgccg cgtcggcgcc gcgattgaaa actccggtga ttttgatttc cggatacttt
      781 ttggtcagtt caccgaaaag cccttcgtat tccgggctgc cgtagagcgt aagttgcccc
      841 tccttcttgg cggcttccag cgtcttatcc cagtctgctt tccaagcctc cgcctgcgcc
      901 gcgagcgcgg cggctgcacc gagcccccaa tacaacgcga tcgtaaaaag ccattttctt
      961 gaatctctca tgagttctcc cgaaaaatat tttcactgtg tttttgcaga gcgtctcgaa
     1021 ctttcaaatt tccatactca attcttcttc gccgcctgcg cctctttaat cactttggac
     1081 accggtgcca tatcaagcca ttcagcacgg ccggcgtaaa tatagcgcac gcccttctgg
     1141 cgccgttgcg acgccggaat aacatccgtg ctgacatctt cgcgcatcga ctggcgaatg
     1201 tcgcccacag aggcgaacac cttttgatag gcctcttgtc cctcgcgcga tagcagccaa
     1261 ttgatcgcaa ttttcgccgc gttgggatga ggcgcgtttt tgaaaaggtt gacgccgccc
     1321 tgagacggac cgacaaacgc gccttctttg aactgactcg gttcgaagtc gcggatcggc
     1381 aatccttgct tggccgcggc ttcgacttcg gtgacgaaaa aggcaatggc gtatttcccg
     1441 gcaccgagcc aatcgatcat ttgccgctcg tcgcgcgaat aggtgagctc catctcactg
     1501 aataatcgcc tgacatattc cgcgccgaga tcggggtgat tgtaaagaaa ccgcagcgaa
     1561 tgagccaccg tgctgacccg attcggatcg tacgcgacca tcttgccttt ccacttggga
     1621 tcgagaaagt cccagtagga tttgatgtcc ttggggttta ccagcttggt gttatacgca
     1681 ttttcgctgc catgaacgtt gccctgaaag acaaaaatat atttggcttc cggatcggca
     1741 taaaggtgtt tgccgctcca ccatttcgac tcatcgacta cttcgggaag gatgagctgc
     1801 gggcggatcg gctcgagaat attggccttg taaaaaaccg tgttgggcgt caccacgcca
     1861 ttgatgaaaa gatcgacgag gtattggcca gcacgccgct cggtcatcac ggcttggccg
     1921 tgctcgttgc cgcgccgatt ggtattcgat tttactttga tctcgggata tttcttttgg
     1981 aatacctcga agatcttctc gtatccttcg ctgccgtaga aggaaacctc tccttctttc
     2041 ttggccgctg tcacagtttt ctcccattca acgctccaag cgtcggccgc tgccgcgaga
     2101 ccattcgtct gaccgatcac ggtcacgaac acgatcacga accacgaaac tacctgcgtc
     2161 ttgctaatca tcgcccgtca ctccatttac tccaacactc gatcaatcca gttttaagac
     2221 gcccttccac ttcgccgccg ccgcccgccg cgtctcctcg ggaaaatagc aagtcttggc
     2281 atactgatct ttccaatggt acggctggca ggcattgatg atcgcgaccg agttagtaaa
     2341 gttgcgcgcc gcgcgcgcgt ccggcgtgat gcgcggatcg agcggcgtgc tccaggcatc
     2401 ggtgatgacg ttgatcgcag tcgccggatc gcagcgcgtc gccagcgccc ataggacatc
     2461 gtcgatgttc gacggatcga cgtcgtcgtc gacgataata acaaagcgcc gatgataacc
     2521 gtaggtcgag ccgagcatgg ccattgccgt gcgcttggcg tgaccgccgt atttctgttt
     2581 gatggagatc gccgttatgt agcgcgtctg gtattcccac acgccctgga cgtcggtcac
     2641 accggctttt tccaacgcca gccacacatt ggcggcgctg agcggcaccg acttgccgga
     2701 gatgcccggc ggcatgccaa aaataatcgg gtcattgcgg taaaaaacgt ttttgacctt
     2761 gaccaccggt tcattgcgcg tgccggaagc gtaatagccg gtccactcgc cgaagggtcc
     2821 ctccgagcgg ctgtcttcct gcggcggcac gatgaagcct tcgatggcca actccgaggt
     2881 cgcgggaatc ggcaacccgg tttctttgcc gcgaatatat ttgaccggtt cgccgaatag
     2941 ccagccggcg atttccaatt ccgacacgcc ccaggcctcg taccaactcg ccactttggc
     3001 aagaatcgga tcttgaccga aactaatcac caccgggcat gacttgcctt gtgcccaata
     3061 tttttcccga atcagccgtc catgtttacc cggagaaatc caaattccca acgtgttttt
     3121 gtcgtgcacg cagacacggt aagtgccgac attggtccac ttgccgtcgg ggtcttccat
     3181 aatcaccatg cagccggtgc caatgtaacg gccgccgtct tcctcatgcc agcgcggcgt
     3241 cgggaacttg agaagatcga ccttgtcgcc gctatcgcag ttctcggtga ttggtccggt
     3301 ggaaacttcg acgggcttga gcggcgtctg cttgccgagc cgctctttcc actggcgaat
     3361 aatatccaag ccattccatt taggatccaa accgaacgcc aacgccgtcg tcgcggctga
     3421 acaaaccgga ttcgtcacca cgcgaaagcc tttgggataa cccttgatct cgtcgaagag
     3481 taccactttc ggattgttcg cccgcgccgc cacttcggta atcgcaccaa gttccaaatc
     3541 ccaatccgcg cccctcacat gacgcagctc tccgagcttg tcggccgcgt caaggtagcc
     3601 gcgcagatct ttgtaaacga gtgaagtttc atttcccatt gtcagatcct tgtccgcgtc
     3661 cggcatccgg tcattttcaa ttctccatta tccattctcg attatccagc aaagcttcag
     3721 cggatgacct tatcggcccg ataca
//
LOCUS       DATLCH010000084         3438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10571534, whole genome shotgun sequence.
ACCESSION   DATLCH010000084 DATLCH010000000
VERSION     DATLCH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10571534"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1077
                     /locus_tag="VMZ02_02470"
     CDS             94..1077
                     /locus_tag="VMZ02_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70820.1"
                     /translation="MKLWLATIFFLTLAPSLVQSQSLDQLIAGAKNEGKLVIYASAAA
                     QQLQMYMAAFNKRYPFIKTEYFRTDKQKLVTRVLLEEQAKQRLADVVHTSVIETHILK
                     KRGALSRYVPMEAASYPAQYKDPEGYWTSVYASGTLMGFNTRQVKRAEAPKTYEDLLN
                     PRWKNMIAVDNTKIEWFSMLLKLKGRGFMEKLAAQNGPVMAGNTNLLNLLAAGEFAIM
                     AGVYEYSVDSLKSKGAPVDWIGLEPVITYTVAASLPSQPAHPFAAKLFIEWLLTKEGQ
                     EVINQYGRVPIRDDIDSKYGKILKQHKLLMTDVDLSQREVEVNEMIRKLFR"
     gene            1314..>3438
                     /locus_tag="VMZ02_02475"
     CDS             1314..>3438
                     /locus_tag="VMZ02_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUR70821.1"
                     /translation="MESNTIATEQLHTYCAMCTSRCGVIATVEDGRLTQVNADPDHPN
                     GCICVKGAAAPEIVYSPDRLQHPLVRTRAKGDANPGWVEITWDQALSLVALRLMNIKD
                     EYGAEAVVFSRATTAGSAAIDFDGWVQRLANAFGSPNLLTSNHICTWNRRVGSKYTYG
                     STMPLPDFDNTQCMLIWGINPPATSPAQAMRISRARNRGAKLIVIDPRKTTLAAKADC
                     WLRVRPGKDGELAMAMIHVMLEEDLFDAAFAQTWTNAPFLVRADNQRLLSAADLRANG
                     DCGTWMVWDEQIGLTAYDQAKLPRLFGSYQINLHDGKTVECRPALQLLKEAAAPFAPE
                     RSEQITTVPAAEVRKAVRLFAHEKPSSYCTWVGLEQDNDAMQTNRAVCILYALTGQYD
                     ECGSNVLFATTPTNPINGRELLPKEQATKRLGLDQHPLGPPADPGLVQAGTVYDAILT
                     NKPYPVKAAVLFGTDPLLGHGDPLHGKAALEALDFYVHVDTTVNPSAHFADVILPATT
                     CWEREALLPFSEISEDTMNWAQLRPAVAKPVGESRSEAEIIFDLARRLDLREYFFDGD
                     IESALAHQLAPSDLTTRQLRASPIGMRASVTTRHKKYAETHSGSGQSKGFNTPTGKIE
                     IYSTAFADAGYPPIPTLMNDESANIEYPLTLTFFRDIHFCDQQHRNISRLRRVIPEPF
                     VEIYPTTASANGIANGDWIFLETETG"
BASE COUNT          858 a         1037 c          885 g          658 t
ORIGIN      
        1 taaaggaaaa aattgccaat gtcgacgcgc ttggttgcaa gcgtgttgac acagccgagc
       61 gcgctcatta ccgtgctcga catcggaggt tcaatgaaac tttggctcgc aacaatcttt
      121 ttcctgacgc tggcgccgtc ccttgtccaa tcgcaatcgc tcgaccaact gatcgccgga
      181 gcgaagaacg aaggcaaact ggtgatctac gcttccgccg cggcacagca gctgcaaatg
      241 tacatggcgg cattcaacaa acgctatcct tttatcaaga ccgaatactt ccgtaccgac
      301 aagcagaaac tggtcacccg cgttcttctg gaagagcaag ccaagcaacg tctggcggac
      361 gtcgttcata ccagtgtgat tgagacccat attttgaaaa aacgcggcgc attgagccgt
      421 tacgtgccga tggaagcggc gtcctaccca gcgcaataca aagacccgga aggttactgg
      481 acatcggtct atgccagcgg cacgctgatg ggcttcaaca cccgccaggt caaacgcgcc
      541 gaagcgccga agacttacga agatttgttg aatccgcgct ggaagaatat gatcgccgtc
      601 gataacacga aaatcgagtg gttctcgatg ctgctcaagc tcaaaggccg cggcttcatg
      661 gaaaaactcg ccgcccaaaa cggccccgtg atggccggca ataccaactt gttgaacctg
      721 cttgccgcag gcgagtttgc cattatggcg ggtgtttacg aatattccgt cgacagtttg
      781 aaaagcaaag gcgcgcccgt cgactggatt ggcctggagc cggtgatcac ctacaccgtc
      841 gccgcgtcgt taccgtcgca gccggcccac ccgttcgccg ccaagctctt tatcgaatgg
      901 ctgctcacca aggaaggcca ggaagtaatc aatcaatacg gccgcgtgcc gatccgcgat
      961 gacatcgatt ccaagtacgg caagattttg aagcagcaca agctgttaat gaccgacgtc
     1021 gatctgagcc aacgcgaggt cgaagtcaac gagatgatcc gcaagttgtt ccgctaagtt
     1081 tgggaagaaa aattaatctc accacaaaga gcacgacaca gacgaagatt tggagatgaa
     1141 gctccgtcat accggcgaaa gccggtatcc attccctcat tgccctggat gccggacttc
     1201 gccggcatga cgaaacaaga gacgaagcat atgacggtaa gagcgctaca atttcatact
     1261 ctgtagacga gcgtatgcta atggacaatt cgtggtgaag cccctctgcg cttttggaga
     1321 gtaacaccat cgccacggaa caactccaca cttattgcgc catgtgcacg tcgcgctgcg
     1381 gagtcatcgc gacggtcgaa gacggacgtc tcacacaagt caacgccgac ccagaccacc
     1441 ccaatggttg catctgtgtg aaaggcgccg ccgccccgga gatcgtctac tcgccagacc
     1501 ggctgcaaca tccgctggtg cgcacgcgtg ccaaaggcga cgccaatccc ggctgggtag
     1561 aaatcacttg ggaccaagcg ctcagcctgg tcgcgctcag gctcatgaat atcaaagatg
     1621 agtacggcgc cgaagcagtg gtgttttcac gcgccaccac tgccggaagc gcggccatcg
     1681 acttcgatgg ttgggtgcag cgactagcca atgccttcgg cagtccgaat ctattaacat
     1741 ccaatcacat ctgcacctgg aatcgccgcg ttggttcgaa atacacttat ggcagcacca
     1801 tgccgctccc cgacttcgac aatactcaat gcatgctcat ctggggcatc aatcctcccg
     1861 cgacctcgcc ggcccaagcc atgcgcatca gccgcgcgcg caaccgcggc gccaaactca
     1921 tcgtcatcga tccgcgcaaa acaactttag cggcaaaggc cgactgctgg ctgcgcgttc
     1981 gtccaggcaa agatggcgag ctcgcgatgg cgatgatcca tgtaatgttg gaggaagacc
     2041 ttttcgatgc cgctttcgcg cagacgtgga ccaacgctcc attcctagtc cgcgccgaca
     2101 accaacgatt acttagcgca gccgatctcc gcgctaatgg agattgcggc acatggatgg
     2161 tgtgggacga gcagatcggg ttaacggcgt atgaccaggc aaagctgcct aggctgttcg
     2221 gtagttatca gatcaatctg cacgatggaa aaactgtaga gtgccggccg gcactccagt
     2281 tactgaaaga agctgccgcg ccgttcgcgc cggagcgaag tgagcagatc accacggtac
     2341 ccgcagcaga agttcgtaag gccgtccgac tattcgcgca cgaaaaacct tcgagttact
     2401 gcacctgggt gggcctcgaa caagacaacg acgccatgca aaccaaccgc gccgtgtgca
     2461 tcttgtatgc gctcaccgga cagtacgacg agtgcggcag caatgtgctg ttcgcaacga
     2521 cgccaacgaa tccgattaat ggccgcgaat tgttaccgaa ggagcaagca accaagcgcc
     2581 tcggtctcga ccaacacccc ctcggcccgc ccgccgatcc cggactcgtc caagctggca
     2641 cggtttacga cgcaatcctc acaaacaaac cgtaccccgt caaagccgcg gttttgttcg
     2701 gcaccgatcc gctcctcggc cacggcgatc cgctgcacgg caaagcagcc ctcgaagcgc
     2761 tcgacttcta cgttcatgtc gacacgacag taaatccgag cgcacacttt gctgatgtaa
     2821 tactgccagc caccacatgc tgggagcgtg aagcattgct gcccttctcc gagatttcag
     2881 aggatacgat gaattgggca cagctgcgcc ccgccgttgc caagccagtt ggtgagtcgc
     2941 gctccgaagc ggaaatcatc ttcgacctgg ccagacgact cgacctgcgc gaatatttct
     3001 tcgatggaga tatcgagtcc gccctcgctc atcaattggc gccgtcggat ctaacaaccc
     3061 gacaactgcg agcaagccca atcggaatgc gcgcgtcagt cacaacacgg cataaaaaat
     3121 atgccgaaac acattccggg agcggtcagt ccaaaggttt caacacacca acaggcaaga
     3181 tcgaaatcta ttcgacagca ttcgcagatg ccggctaccc gcccataccc acgttgatga
     3241 acgatgaatc agcaaacatc gagtaccctc tcacactaac attctttcgc gatatccact
     3301 tctgcgatca acaacaccgc aacatttcgc gcctgcgccg cgtcatacca gaaccgtttg
     3361 tcgaaattta tccgacaacc gcgtcggcaa atggtatcgc caacggcgac tggatttttt
     3421 tggaaacgga aaccggca
//
LOCUS       DATLCH010000085         4014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2511910, whole genome shotgun sequence.
ACCESSION   DATLCH010000085 DATLCH010000000
VERSION     DATLCH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511910"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VMZ02_02480"
     CDS             <1..579
                     /locus_tag="VMZ02_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70822.1"
                     /translation="AITLIVLGLAFWFFTRRKTTEHLRERFGPEYDNTVRTLRSQQRA
                     EAELTKREERVSKLQIVTLPRPERDRYQENWLAVQSRFVDEPERALGDAYRLVQDVMK
                     RCGYPVADFEQSAADISVDHPHVVENYRAATRIVDRSERSAASTEELRQALVHYRALF
                     DDLLHSDAPDGGIKQTGKRIRQSKSSNSHPSH"
     gene            597..1040
                     /locus_tag="VMZ02_02485"
     CDS             597..1040
                     /locus_tag="VMZ02_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70823.1"
                     /translation="METAETKKLTTKDMARAENDQAVFRHDKQEQEQQREAEARDDRE
                     QRNPAPSPATNGESPSPLLSADQGKDFRSRWQNVQVGFVDEPRRAVEQADELVAELMQ
                     HLARSFSDQRSRLESEWEHSEKVSTEELRLALRRYRSFFDRLLSV"
     gene            complement(1105..2931)
                     /gene="cysN"
                     /locus_tag="VMZ02_02490"
     CDS             complement(1105..2931)
                     /gene="cysN"
                     /locus_tag="VMZ02_02490"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HUR70824.1"
                     /translation="METLRFITCGSVDDGKSTLIGRLLFETKLIFEDQLEALKADSKK
                     FGTQGERPDLALLVDGLQAEREQGITIDVAYRFFATSKRRFIVADTPGHEQYTRNMAT
                     GASTAEAAVLLIDARKGVLTQTKRHSRIAAMFGVRHIVLAVNKMDLVEWSQARFEEIT
                     ADYRQFAQALNFANVTAIPLSALEGHNVVDSAAPTMPWYHGPSLLQWLEIVPCGGRES
                     AQPFTMPVQWVNRPNLNFRGFSGRIASGSIRPGDSVRISPSGVVSRAKEIVVWGGSVS
                     AAGAGASVTLVLESEVDASRGDVITAADRPVEVADQFEAQILWMSEHPLLPGRSYAAQ
                     IHSKAANVTITEIKHCLDINSGAHLAAKMLGLNEIATVNVSFDRPVTYAPYDDNRRLG
                     AFILIDKLTHETVGAGLIHFALRRAANVHWQALEVTRAARAVLMGQQPRCLWFTGLSG
                     SGKSTIANLLDKRLHAEGRHTYILDGDNVRHGLNRDLGFTEADRVENIRRIGEVAKLL
                     VDAGLIVIVSFISPYRAERQLARDLFQPGEFVEIYVDTPLEECERRDVKGLYAKARRG
                     ELKNFTGIDSAYEVPEDPEIHLRTMDLNPVVCVTRILGWLQP"
     gene            complement(2932..3834)
                     /gene="cysD"
                     /locus_tag="VMZ02_02495"
     CDS             complement(2932..3834)
                     /gene="cysD"
                     /locus_tag="VMZ02_02495"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HUR70825.1"
                     /translation="MNRMNVFSELEAESIEIFREVAATAARPVMLYSIGKDSSVMLRL
                     AQKAFYPGPIPFPLLHIDTGWKFREMIAFRERIAEELGVELLRYTNPRGVAEGIGPFT
                     HGSAYHTDVMKTEALKQALDLYGFDAAFGGARRDEEKSRAKERIFSFRAAGHRWEPKT
                     QRPELWNLYNTRINPGESIRVFPLSNWTEADIWGYIQQEQIPIVPLYFAAPRPVVKRY
                     GQWIMVDDERMPLEPGEVPEMRVVRFRTLGCYPLTAAIESSAATLAEIVAETLGANTS
                     ERQGRVIDHDGAGSMERKKQEGYF"
BASE COUNT          940 a         1138 c         1108 g          828 t
ORIGIN      
        1 gcaattacat tgattgtgct cggcctggca ttttggtttt tcacacgccg caaaaccacc
       61 gaacatttac gcgagcgctt cggacctgag tacgacaata cagtcaggac actgcgcagc
      121 caacagcggg cggaagccga gttgactaaa cgcgaggaga gagtcagtaa attacagatc
      181 gtcacgttgc cccgaccaga acgcgatcgc taccaggaaa attggttggc ggttcaaagt
      241 cgctttgtcg atgagccgga gagggcgctc ggggatgcgt accgcctagt acaagatgtt
      301 atgaaacggt gcggctatcc tgtggccgat ttcgaacagt ctgcggcgga catttcagtt
      361 gatcaccccc acgttgtcga aaactatagg gcagccactc ggatcgtcga ccgtagcgaa
      421 cggtctgccg ccagcacaga agaactgcgg caagcgttgg ttcattaccg agctttgttt
      481 gacgatctgc tccactcgga cgctcccgat ggcggtatta aacaaaccgg caaacgaata
      541 cggcaaagca aatcctcgaa cagtcatcca agccactaaa cggaggtaaa gtcacgatgg
      601 aaacggcgga aaccaagaaa ttaacgacca aggacatggc gcgagccgag aacgatcaag
      661 cggtttttcg ccatgacaag caggaacagg agcaacagcg cgaagcggaa gcccgggatg
      721 acagagagca acgaaaccca gcgccatcgc ccgcgaccaa tggggaatct ccaagtccat
      781 tgctttctgc agatcaggga aaagatttcc gctcgcgttg gcaaaatgtc caagttggtt
      841 ttgtcgacga gccacgccgg gcggtggaac aagcagacga gctcgttgcc gaacttatgc
      901 agcacctggc acggagcttc agtgatcaga gaagccgcct cgaatctgag tgggagcatt
      961 ccgagaaggt ctcaaccgaa gaactgcgtc tagcactgcg ccgttatcga tctttttttg
     1021 accggctact ttcggtgtag aagcaaccgg aagctttctg cgggcacgca gttagcaggt
     1081 ccaacgaaaa ttcggacttt tctgtcaggg ttgcaaccac ccgagaattc gcgtgacgca
     1141 gactactggg ttgagatcca tggtccgtaa gtggatctcg ggatcttcgg gaacctcata
     1201 cgcgctgtcg atgccggtga agttttttaa ctctccgcgc cgcgccttgg cgtaaagccc
     1261 ctttacgtcg cgacgttcgc attcttcaag cggcgtatcg acgtatattt cgacaaactc
     1321 gccgggctga aacagatcac gggcgagttg gcgttctgca cggtacggag aaataaacga
     1381 aacaatgaca atcaggcccg cgtcaactag caatttcgcc acttcgccga tgcgccgaat
     1441 gttttccacc cggtccgctt cggtaaatcc gagatcgcga ttgagtccgt ggcgcacatt
     1501 gtcgccgtcc agaatgtagg tgtggcgacc ctccgcgtgt aaccgcttgt ccagcaggtt
     1561 tgcgattgtc gatttgcctg accctgacag gccggtaaac cacaggcaac gcggctgttg
     1621 tcccatcaaa accgcgcggg ccgcccgggt aacttccaga gcctgccagt ggacattggc
     1681 ggcgcgccgg agcgcaaagt gaatcaaacc ggcgccgacg gtctcatggg tgagcttgtc
     1741 gatcaagata aatgcgccca agcgacggtt gtcgtcgtac ggcgcatacg tcaccggacg
     1801 gtcgaagctg acattgacgg tagctatttc gttgagaccg agcattttcg cggctaggtg
     1861 ggcaccactg ttgatatcca gacaatgctt aatctcggtg atcgtaacgt tagcggcctt
     1921 gctgtgaatt tgcgcggcat aagagcggcc cggcaataaa ggatgctcgc tcatccaaag
     1981 aatttgcgct tcgaactgat ccgctacttc gacgggccga tcggccgcgg taatgacatc
     2041 gccgcgacta gcatcgactt cactctccag taccaacgtc accgaagcgc cggcacccgc
     2101 cgcgctcaca gatccacccc agacgacgat ttcctttgcc cggctgacaa caccggacgg
     2161 actgatacgt acactatcgc ctggcctaat cgacccgcta gcgatacggc ccgagaaccc
     2221 acgaaagttt agattcggtc gattaaccca ttgcacgggc atagtgaagg gctgcgctga
     2281 ttcgcgcccg ccgcacggaa cgatttccag ccattgcagc agagacggac catggtacca
     2341 cggcatcgtc ggagcggctg agtcgacaac gttatgccct tcgagcgcgg acagcgggat
     2401 cgccgtgaca ttggcgaaat tcaaagcttg ggcaaattgc cgatagtcgg cggtaatctc
     2461 ttcgaaccga gcttgtgacc actccaccag gtccattttg ttaaccgcta gtacgatatg
     2521 acggacaccg aacatcgccg caatccggct atgccgcttg gtctgcgtga gcacgccctt
     2581 acgcgcatcg atcaacagca ccgccgcctc ggcggtggac gcgccggtgg ccatattgcg
     2641 ggtgtattgc tcgtggccgg gagtatcggc aacgatgaag cgccgcttcg aggtcgcaaa
     2701 gaaccgataa gcgacgtcga tggtgatgcc ctgctcccgt tcggcctgta gaccgtcgac
     2761 cagtagagcc aaatcggggc gctcgccctg cgtgccgaat tttttcgaat cggctttgag
     2821 cgcctcgagc tgatcttcaa agattagttt ggtctcgaat aataagcggc cgatcaacgt
     2881 cgacttgcca tcgtcgacac taccgcaagt aataaagcgc agggtctcca tttagaaata
     2941 cccttcctgc ttcttgcgct ccatggagcc cgcgccgtca tgatcgatga cgcgtccttg
     3001 gcgttccgaa gtgttggcgc cgagagtttc ggcgacaatt tccgcaagag tggcggcgct
     3061 gctttcgatc gctgccgtca gcggatagca acccagcgtg cgaaaccgca ccacgcgcat
     3121 ttcgggcact tctccaggct caagcggcat gcgctcgtcg tcgaccatga tccattgacc
     3181 atagcgtttg accaccggcc gtggagccgc gaaatagagc ggtacgatgg gaatctgctc
     3241 ctgctgaatg taaccccaaa tgtccgcttc ggtccagttt gacaagggaa agactcgtat
     3301 cgattcgcca gggttaatcc gcgtgttata gagattccaa agttccgggc gttgagtttt
     3361 gggctcccac cggtgcccgg cagcacggaa cgaaaaaatt ctttctttag cccgtgattt
     3421 ctcctcatca cgccgagccc cgccaaaagc cgcgtcaaag ccgtagagat cgagggcttg
     3481 cttgagcgct tcagtcttca tgacatcggt atgataggcc gagccatgag taaacggccc
     3541 aatgccttcg gcgacgccgc ggggattagt gtagcggagc aattcgacgc cgagctcttc
     3601 ggcgatccgc tctcggaaag caatcatctc acgaaatttc cacccagtat cgatatgcag
     3661 caatggaaag ggaatcggtc ctggataaaa cgccttctgc gccagacgca gcatcacgga
     3721 cgagtccttg ccgatggagt agagcatcac cggccgagcc gcggtggccg caacctcacg
     3781 gaagatttcg atggactcgg cttcaagctc agaaaaaacg ttcatacggt tcatgcgttc
     3841 gcctacccca acgacttgtc agttaatcac gccctacgta ccgataatcg tcgtgaggtc
     3901 gaccctgagg gaccaagtgc agacgattac ctgcaccgat cacaaattac atagtttcgg
     3961 tattttgaca aaacttagag tacgaaaagt ttgcctgacg acttgtacag gcgg
//
LOCUS       DATLCH010000086        11316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5495952, whole genome shotgun sequence.
ACCESSION   DATLCH010000086 DATLCH010000000
VERSION     DATLCH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11316
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5495952"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..257)
                     /locus_tag="VMZ02_02500"
     CDS             complement(90..257)
                     /locus_tag="VMZ02_02500"
                     /inference="COORDINATES: protein motif:HMM:NF010229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HUR70826.1"
                     /translation="MLNYALTFLVIGIIAGLLGLAGVASVATQIGWILFLVGLVLLLV
                     HLFRGRVPPVA"
     gene            complement(311..535)
                     /locus_tag="VMZ02_02505"
     CDS             complement(311..535)
                     /locus_tag="VMZ02_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70827.1"
                     /translation="MKKYLLLIVLAAMTQQGCAFLGGAALGTLATGGGYEINNKRQMD
                     RLDEDLRNERITRRQYEERKQQIEKGSIIY"
     gene            complement(727..2616)
                     /locus_tag="VMZ02_02510"
     CDS             complement(727..2616)
                     /locus_tag="VMZ02_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR70828.1"
                     /translation="MAPAFVSALGPPTLRTHLVKLIFIGLAPLIIFSAILIVLFARQE
                     QVTLTLGLQETTRALSAAVEKEFQANITVLEALASLEALDAGTVQEFSRSAWRVLNGQ
                     PNWAGVALFDTTGRPLLANTKPGVSLAALDRGTLDQVVQHKRPSVGGFSGGLDGRVQI
                     SVPVVRENKVIHVLTAALGRDVFAAILMRQKLPADWIGTIIDDRRVVVARTLAPEVSV
                     GQPAGPLLQQVPGGEKIFTGKTREGIFSYAAVSTLPYFGWSVALIVPATQVSGLLWRS
                     LASIIGAAVLCCLAGFWLASRFARGLADPIRKLSESAKALGEGRLNETPPALAIVELN
                     DVSHNLQRAAELLREQSRLRDRIEAELRDRDEFLERQAQLLRNNEEVLRRQAGELEQQ
                     LLASGRLVAVGELTASMAHEFNNPLGIILGFAQGLLAEMDPADANFHHVQIIAEEARR
                     CEKLVQELLEFGRPRKAEFTELDVKPIVEKTLDLISNRAAKSRVGTSVSVADDLPRVR
                     ADMQQIQQMLLNLSLNAIDAMPQGGQLTLGAAKNGSSGIMLIVADTGAGIGSEMLPRI
                     FQPFYTANKRRGLGLGLPICNRIAKAHGGSISVESQPNRGTTFRIHLPINADGDVSAT
                     AEQPE"
     gene            complement(2669..3070)
                     /locus_tag="VMZ02_02515"
     CDS             complement(2669..3070)
                     /locus_tag="VMZ02_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR70829.1"
                     /translation="MKSLAADPSRQTSEVPGKAVLVVDDEQNFLSLLHWFLSNRGYAV
                     ETAASAEQALELLEARTFELALIDIRMGPMDGLTLLAELKRRMPMMRVVVMTAYPTGG
                     AIRQSHEKGASAFLTKPVDLQELMKTIQSLV"
     gene            complement(3100..3273)
                     /locus_tag="VMZ02_02520"
     CDS             complement(3100..3273)
                     /locus_tag="VMZ02_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70830.1"
                     /translation="MEQQDVQDVEVTINEVISVLRENNAATAVNQRETNILAAYMLCR
                     LFGSEGICSRSWH"
     gene            complement(3479..4084)
                     /locus_tag="VMZ02_02525"
     CDS             complement(3479..4084)
                     /locus_tag="VMZ02_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70831.1"
                     /translation="MKKHLSKILAMVGLIALMASAACENIAIIKRPTPQLDGDEIVGE
                     ITGIDLRVNQIYLRSTEDGRRATPQTIRLEENTQLLYRGRDYPLSSLEVGDIVALQVR
                     DRQRGSSTNLLRLEQSSKDRQARQSGAGSQRIEGTVERIDLDSGAFELRERSGNNVRV
                     SLSPNAHAPVAEQVHRLRRGDQVLAQGRFLSRDRFELEELL"
     gene            complement(4191..4610)
                     /locus_tag="VMZ02_02530"
     CDS             complement(4191..4610)
                     /locus_tag="VMZ02_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HUR70832.1"
                     /translation="MRPTKSTSWFTSFAKAISRHTGRPVVFIIAFSTVVVWALTGPLF
                     GYSDAWQLVINTSTTIITFLMVFLIQNTQNRDSEAIQIKLDELIRVSKGAQNALLNLE
                     ELEEAELDVIRQEYVQMAEAARTEWRRKPGKPGHAKR"
     gene            complement(4893..5087)
                     /locus_tag="VMZ02_02535"
     CDS             complement(4893..5087)
                     /locus_tag="VMZ02_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70833.1"
                     /translation="MEFTCRHCNELSEGDAYRVSSEENGVVLLDIIVCFRCAMEARGL
                     ELSTRKIDLPSEEMRQALFH"
     gene            complement(5286..8660)
                     /gene="treS"
                     /locus_tag="VMZ02_02540"
     CDS             complement(5286..8660)
                     /gene="treS"
                     /locus_tag="VMZ02_02540"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HUR70834.1"
                     /translation="MSDKSQTLAQAAVEEKLPLTRAERARWYKDAIICEIHVRAFSDG
                     NGDGIGDFKGLTEKLDYLQDLGITALWLLPFYPSPLRDDGYDIADYTDIHPSYGTLND
                     FRTFLKAAHSRGLRVITELVINHTSDQHPWFQRARRAPAGSKARDFYVWSETQEKYQD
                     ARIIFKDFETSNWSWDPLAKAYYWHRFYSHQPDLNFDNPAVQKAIFAALDFWLGLGVD
                     GLRLDAVPYLFEREGTSCENLAETHGFLKVLRRHVDERYRDRMLLAEANQWPEDAAAY
                     FGSGDECHMAFHFPVMPRLFMAVHMEDRYPIIDILQQTPQIPELSQWALFLRNHDELT
                     LEMVTDEDRDYMYHVYATDPQSRINLGIRRRLAPLLAGNRRKMELLKSLLFSLPGTPV
                     LYYGDEIGMGDNIYLGDRNGVRTPMQWSADRNAGFSRANPQRLYLPVIIDPEHHYETV
                     NVEAQQQNLSSFLWWMKRLIVLRKRYLAFGRGTMEFLYPANHKVLAFIRRYGDEVILA
                     VANLARFAQYVELDLAQFKGMVPLELFGQSRFPSIGAVPYFLTLSPHGFYWFKLETPK
                     VIERNNQGTSAVAIKIASSWEELFDLRSGTTALTDALPEYLGRCRWFGGKARRIRLIS
                     LIDTIPMRFSGTVVYVSTVQVQYTEGPAEEYFLALGFADGARAAELRQGRGPAIVAQL
                     VKEDDGASAGVLYDALTDRDFAQSMLDSIARRRSLKGEKGELRGVPTRALRKLIAPPL
                     IIPPATVVKGEQSNTSVVFGDRLVLKFFRRLHSGINPDLEIVRFLNEKTAFRNLPPLA
                     GHIELRCPGEEPKLAAILQGWVVHETDGWSYTLDNLKQYFEHCLARKYEESELPVPRR
                     HLIDLADGETPGAAQAAIGGYLALAGLLGQRTAEMHLALASVSDDVNFSPEPFTPHYR
                     HSLYQSLRTFAYKTFSLLRERMANIPEGCRAQAADVLAAENRLMGRYHVLIERKIAAV
                     RLRCHGDFHLGQVLFTGKDFVIVDFEGEPARTLGERRIKRSPLRDVAGMLRSFDYATI
                     VGLRTNGHRSDDVVALAPWMRLWNLWVSVAFLRRYLQIAGRGSFLAKSREDLKTLLDI
                     LVLDKAVYELNYELNNRPDWVDVPLRGILELLNNDS"
     gene            complement(8664..10643)
                     /locus_tag="VMZ02_02545"
     CDS             complement(8664..10643)
                     /locus_tag="VMZ02_02545"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HUR70835.1"
                     /translation="MMDRRRVIIEGVKPEIDGGRFPAKRTVGESVCVEADVFVDGHDQ
                     VAATLLTRHSSQTNWSESPMEPRANDRWRAWFRVGELGRYYYSIRGWLDVFATWRRGL
                     AKKIADGQQDPVELLIGALLIDGAAARADKNAANALRRWAELMRRRDDAAWELAGTQA
                     DELGQLMARYPDLRYATQYERDLVVLVEREKAGFSAWYELFPRSCGRAGAHGTLADCA
                     ARLPYVASMGFDTLYLPPLHPIGRSHRKGKNNSLLSMPGDPGSPWAIGAAEGGHKAIH
                     PELGTLEDFDRLLVTAGELGLEIALDLAYQCSPDHPYVTAHPEWFGHRPDGTIQYAEN
                     PPKKYQDIYPFNFESEDWRELWQELESIARFWLERGVRIFRVDNPHTKPFGFWEYLIA
                     ALQQDYPDAIFLAEAFTRPKVMRRLAKLGFSQSYSYFTWRNTSSELTDYFSEITGSDA
                     REYFRANLWPNTPDILPEYLQYGGRPAFMARLVLAATLAANYGIYGPAFELCEARARE
                     PGSEEYLDSEKYQLRQWNLDDPASLKDFITRINRIRRENTALHNDRSLSFHPTDNEQL
                     LCYSKTSADSENIIIVAVNLDPHHKQSGWATPNIDELAVDGKRGYQLHDLISDSRYFW
                     HGSRLYLELDPAIVPAHVFRLRRHLRREQQFDYFM"
     gene            complement(10640..>11316)
                     /locus_tag="VMZ02_02550"
     CDS             complement(10640..>11316)
                     /locus_tag="VMZ02_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70836.1"
                     /translation="AFHGAINSLAQTVLKIAAPGVPDFYQGTELWDFSFVDPDNRRPV
                     DFSLRRRILADLMELERNNPQALLDRLLDSPRDGAIKLYVVYKGLQLRRNQARVFLQG
                     EYLPLAVAGEMTEHVCAFARCYDSQWVVAVVPRLLAEYGASLAELRDLWAGNELLLPP
                     LAGLRWSNVFTGETVRAHMTAKGQALSLSEIFARFPVALLQAEASADGPLTFPQIEES
                     ALGIVP"
BASE COUNT         2538 a         3229 c         3085 g         2464 t
ORIGIN      
        1 aatagcagca aaatggcgaa tgggagaccg acaatccagc cgttcgacaa catggccacg
       61 gcctcccgtc cgacgtcact gccaatgtgt catgctaccg gtgggacgcg cccgcggaag
      121 agatgcacca gtagtaaaac aagtcccacg agaaacagga tccagccgat ctgggttgcc
      181 acagaggcaa ctcccgctaa gccgagtaat cctgcaataa tcccaatcac caaaaatgtc
      241 agtgcataat taagcatgac tcctccttgt ttgattcact cataactacc ccatgcgcag
      301 cccaaggtag ttagtaaatg atcgaacctt tttcgatttg ctgcttgcgc tcttcgtatt
      361 gtcgccgggt aattcgttcg ttgcgcaggt cttcatccaa cctgtccatc tgtcgtttgt
      421 tattaatttc ataccccccg ccggtcgcca atgtacccaa cgccgcacca cctagaaagg
      481 cgcacccctg ttgggtcatt gctgcgagga cgattaaaag taaatatttc ttcataacta
      541 tcaaatctcc ttttctctaa acttgactgt agttagagca atcgtgatgc catcgggaga
      601 cttcggataa gcctgagatt taacagaaat tgcccaagta aaattagttt tttccgattt
      661 tgggacaagg aaaatctact agaaggtttt tctctgacac tgccgtttaa ctaataaaaa
      721 cagcgattat tctggttgtt ctgcggttgc tgaaacgtcg ccgtcagcat tgatgggaag
      781 atgaatccgg aaagtcgtgc ctcggttcgg ctggctttcc acggaaatgc taccgccgtg
      841 cgccttggca atgcgattac agatcggcaa accaaggccg agaccgcgcc gcttgttggc
      901 ggtatagaac ggctggaaga tgcgcggcag catctcgcta ccaatacccg cgccggtatc
      961 ggcaacgatc aacatgatac cgcttgaacc gttcttggcg gctcctaaag taagctggcc
     1021 gccctgcggc atcgcatcga tagcgttcaa gcttaaattg agtaacatct gctgaatttg
     1081 ctgcatgtca gcgcgtactc tcggtaaatc atcggcgacg ctgaccgacg tgccgacgcg
     1141 acttttagcg gcgcggttgg aaatcaagtc taaagttttc tcgacgatcg gtttcacgtc
     1201 gagttcagtg aattccgcct tgcgcggacg gccgaattca agcaactcct gcacaagttt
     1261 ctcacagcgc cgtgcctcct cggcaatgat ctggacatgg tggaaattag catccgcggg
     1321 atccatctct gcgagcaatc cttgggcgaa acccagaata atacccagcg gattattgaa
     1381 ctcgtgggcc atagacgcgg taagttcgcc aacggcaact aaacgacccg acgccagtaa
     1441 ctgctgttct agttctccgg cctgccggcg cagcacctct tcattgtttc gcaaaagctg
     1501 cgcttggcgc tcgagaaact cgtctctatc gcgcaactcg gcttcgatgc gatctcgcag
     1561 ccgagattgc tcacgcaaca gctctgcggc acgctggaga ttgtgtgaca catcgtttaa
     1621 ctcgacgatc gccaatgccg gcggagtttc atttaagcgc ccctcgccca acgccttcgc
     1681 cgattccgat agctttcgta ttggatcagc caggcctcgt gcaaatcgcg atgccaacca
     1741 aaaccccgca aggcagcaga gcactgcggc acctatgatg gaagccaatg agcgccacaa
     1801 caggccgctt acctgcgtcg ccggcacgat cagcgccacc gaccagccaa aataaggcag
     1861 ggtgctcacc gccgcatagg aaaaaattcc ttcacgagtt ttgccggtaa agattttttc
     1921 tccgcctggg acctgttgca gcagcggccc cgcgggttgg ccaaccgaca cttccggcgc
     1981 gagagtacgc gcgaccacga ctctgcggtc atcgatgata gtgccgatcc aatcggcggg
     2041 aagtttttgg cgcatgagga ttgcggcgaa gacatcgcga cctagtgctg ccgttagcac
     2101 atgaatgact ttattctcgc gcaccaccgg cacagagatt tgcacccggc cgtcgagccc
     2161 gccggagaat ccgccgaccg acggccgctt gtgttgaacg acctgatcga gagtgccacg
     2221 gtcgagcgcg gcgagactaa cgccgggctt ggtatttgcc agcaacggtc gccccgtcgt
     2281 gtcgaaaagc gcgacgccgg cccagttggg ctggccattg agaacgcgcc aggcgctacg
     2341 gctgaattct tgaactgtgc cagcgtcgag ggcttccaac gatgccagtg cctccaatac
     2401 cgtgatattg gcttgaaatt ccttctcgac cgcggcgctc agcgcacgtg tagtctcttg
     2461 cagtccgaga gtcagagtca cctgctcctg gcgggcaaac aaaacgatta atattgcgga
     2521 aaaaataatt aggggtgcca aaccaataaa aattagtttg accagatggg tgcgcaacgt
     2581 gggtgggccg agggcagaaa caaaagcagg tgccataaga gagtaccgca cagcgcaccg
     2641 taaccgaccc attctctcgc ggaatttttc agacgagaga ctggatggtt ttcatcaact
     2701 cctgaaggtc gaccggttta gtcagaaacg ccgaagctcc tttttcatga gactgtctga
     2761 ttgctccacc ggtcgggtaa gctgtcatga ctacgactcg catcatcggc atgcgccgct
     2821 tcaactcggc tagaagcgtc agcccgtcca tcggtcccat tcttatgtcg atcaaagcta
     2881 gttcgaaagt tcgcgcttcg agcaactcta acgcttgctc tgcgctcgcc gcggtttcca
     2941 ccgcgtagcc gcgattgctc aaaaaccaat gaagcagcga aagaaagttt tgttcgtcgt
     3001 cgactaccaa aaccgctttg ccgggtactt cactggtttg ccgcgatgga tctgctgcca
     3061 gtgacttcat ttagccgatc cttcgtcccg ggtcgatcct caatgccagc ttcgcgaaca
     3121 aatcccctcg ctgccaaata gtctgcacag catatacgcc gccagaatat ttgtctcgcg
     3181 ctgatttacc gcagtggcgg cgttgttttc tcgcaacact gaaattactt cattgatggt
     3241 gacttctaca tcttgcacat cttgttgttc cataaaagct cccgagcgtc attaacaacg
     3301 agtgcgcaat tcgcgtgcca cgacagagcg ggtagaaatc gcagcctttg aacaattcag
     3361 gtaccgacaa atcagcggat ttccttcttg aaggagattt tccgatagtc atgctgggac
     3421 agagtaccgc tccaggcggc actcaatcgc atcgcacgcc agctcgaacg cagaaagcct
     3481 aaagtaattc ttctaattcg aaacgatctc ggctcaagaa tcgtccttgg gccaaaacct
     3541 ggtcaccgcg acgcaggcga tggacctgtt cggcgaccgg cgcatgcgca ttgggagaga
     3601 gcgatacgcg cacgttgttt ccggagcgct cgcgcaattc gaacgcgccg ctgtccaaat
     3661 cgattctttc gacagtgcct tcgattcttt gagatccggc gccggattgg cgcgcttgcc
     3721 gatctttact gctctgttcg agtcgaagca gattggtgga actcccgcgc tgacgatcac
     3781 gtacttgcaa cgcgacgatg tcgccaacct ccaacgaact cagcggataa tcgcgaccgc
     3841 ggtagaggag ttgagtgttt tcttccaaac gaatcgtttg cggcgtagcg cggcggccgt
     3901 cttctgttga gcgcaagtag atttggttaa ctcgaaggtc gataccggta atctcgccga
     3961 ctatttcgtc gccgtcgagt tggggcgtcg gccgttttat tatcgcaata ttctcgcagg
     4021 cggcgcttgc cattagcgca atcaagccca ccatcgccaa tattttactg agatgttttt
     4081 tcattatttc ctccgcgctc ggaaggccac gcaagtactt gaagcgcacc tctcgctcga
     4141 ctgactgatc gaatcttgtc ggccagtttt cctaccgcag gcgctccggc tcagcgcttg
     4201 gcgtgacccg gcttgccagg tttacggcgc cactcggtac gcgcagcctc ggccatttgc
     4261 acatactcct gccgaatcac gtccagttcg gcttcctcga gctcttctag attgagcagc
     4321 gcattttgcg cccctttgct gacgcgaatt agttcgtcga gctttatctg aatcgcttcg
     4381 gagtcacggt tctgggtatt ctgaattaaa aataccatca gaaaagttat tatcgttgtg
     4441 ctcgtgttga taaccagttg ccatgcatcg ctgtagccaa acagcggccc ggtgagcgcc
     4501 cacacaacga ccgtgctgaa agcgatgata aacaccactg gtcggccggt gtggcgagag
     4561 atggctttgg cgaaactggt gaaccatgaa gttgatttag tgggtcgcat agctctcctt
     4621 cgcgaagctc gaaccatcag acggtggctt tacatcagct ccatcgccgg cgctaagaat
     4681 tgagattgaa tggccatcgg cggaacgaag ctgggccata cggcttcacg gcaaagccca
     4741 tgccaatgaa ctccggaaag aagcaaaagg atttcggtat cggattaaat aatcttcttt
     4801 gaagatttat ttcttaatga gaaaggcaaa ctttcgaatg gtctaggctg gaaaacaccc
     4861 gtcgtaccaa aaactgccga taaattgcag ttctaatgaa aaagcgcttg acgcatttcc
     4921 tcggatggaa gatcgatttt tcgggttgac aattccaacc cgcgcgcctc catggcgcag
     4981 cgaaaacaaa cgatgatgtc gagcaggaca acgccgttct cctcgcttga aacgcggtag
     5041 gcatcgccct cgctaagttc gttacagtgg cggcatgtga attccatggt tttcgatgca
     5101 gcaaacactg tgccgttctt attcacgata gagtggtaaa cagttcattt atgctgctcc
     5161 agatttgata atttagcacc attgctgttg tggaactgag acaaagcatg ccccaattag
     5221 gttcccgcat tctgcgttta ggagcttaag acacacgcag cagtgagccc aaataccgca
     5281 gcgatctagc tgtcattatt cagtaattcg agaatgccac ggagcggaac gtcgacccaa
     5341 tcgggccggt tgttgagctc gtaattcagc tcgtagacgg ctttgtcgag gacgagaatg
     5401 tcgagcaagg tttttaaatc ttcgcggctt ttggctagaa aactgccgcg tccggcaatt
     5461 tgcagatagc ggcgcaagaa cgcgacgctg acccacagat tccataatcg catccaggga
     5521 gcgagggcga caacatcgtc ggagcggtga ccatttgtcc gcagaccgac gatcgtcgca
     5581 tagtcaaagg accgcagcat accggcgaca tcgcggagcg gcgaacgctt gatgcggcgc
     5641 tcgccaagcg tccgcgccgg ctcgccttca aagtcgacaa tgacgaaatc tttgccggtg
     5701 aacaggactt ggcccaaatg gaaatcaccg tggcagcgca ggcgcactgc ggcaattttt
     5761 ctttcgatca aaacatgata gcgccccatc aagcggtttt ccgccgccag tacgtcggcc
     5821 gcttgagcgc gacacccttc gggaatattc gccatgcgct cgcgcaaaag cgaaaaagtt
     5881 ttataagcga atgtgcgcag gctctgatag agcgagtggc gatagtgcgg tgtaaacggt
     5941 tccggcgaga agttgacgtc gtcgctaacg ctcgccaagg ccaagtgcat ttcagccgtg
     6001 cgctgaccga gcaaaccagc caacgccaga tagccgccga tcgctgcctg cgccgctcct
     6061 ggtgtttcgc catcggcgag atcgatgaga tgacggcgcg ggaccggcag ctccgactct
     6121 tcgtatttgc gcgccaagca atgctcgaag tactgcttca ggttgtccag ggtgtagctc
     6181 cagccgtcag tttcgtgcac gacccagcct tgtaggatcg ccgccaattt gggttcttca
     6241 ccgggacagc gtagttcaat gtgtccggcg agcggcggca ggttgcgaaa ggcagttttc
     6301 tcgttgagaa accggacgat ctccagatca ggattgatac cgctgtgcag tcggcggaaa
     6361 aacttcagca ccaggcggtc gccaaatact accgatgtat tgctctgttc gcctttgacc
     6421 accgtggcgg gcgggataat cagcggtggc gcgatcaatt tgcgcagagc ccgtgtggga
     6481 actcccctga gctcgccttt ctcgcctttg aggctgcggc gccgagcaat gctgtcgagc
     6541 atcgactggg cgaaatccct atcggtcagc gcgtcgtaca aaactcccgc gcttgcaccg
     6601 tcgtcctctt tgaccaattg ggcaacgatc gctggacccc gaccttgacg caattcagca
     6661 gcgcgcgcgc cgtccgcgaa acccagagcc aagaaatatt cctcggccgg gccttcggtg
     6721 tactgcacct gcacggtgct cacgtagacc acagtgccgc tgaagcgcat gggaatggtg
     6781 tcgatcaggc tgatcaatcg gatgcgccgc gctttaccgc cgaaccaacg gcaacggccc
     6841 aggtattccg gcagtgcatc ggtcagcgcg gtcgtgccgc tccgcaagtc gaacagttcc
     6901 tcccacgagc tggcaatctt gatggcgacc gcgctcgtcc cctggttatt cctttcgatc
     6961 acttttgggg tttcgagttt gaaccaataa aagccgtgcg ggctcaaggt gagaaaatag
     7021 ggcaccgctc cgatcgacgg gaaacggctt tggccgaaaa gttccagggg caccatgcct
     7081 ttgaactgcg ccaagtcgag ctcgacgtat tgcgcgaagc gggccaaatt ggcgacggcg
     7141 agaattacct cgtcgccgta gcgccggata aacgccagca ccttgtgatt ggcggggtat
     7201 agaaactcca tcgtcccccg gccaaacgcc agatagcgct tgcgcagaac gatcagccgt
     7261 ttcatccacc acagaaacga gctcaaattt tgttgctgcg cttcgacgtt tacggtttcg
     7321 tagtgatgct caggatcgat aatcaccggc agatagagcc gctgcggatt ggcgcgtgaa
     7381 aagccggcat tgcggtcggc actccactgc atgggtgtac gcacgccgtt gcgatcaccg
     7441 agataaatat tatcgcccat gccgatctca tcgccgtaat ataagaccgg tgttcccggc
     7501 aacgaaaaca gcagactttt cagtaactcc attttgcgcc ggttgccggc gagcaacggc
     7561 gccaaacggc ggcgaatgcc cagattgatt cgcgactgcg gatcggtggc atagacgtga
     7621 tacatgtagt cgcggtcctc atccgtgacc atttccaagg tcagctcgtc gtggttgcgc
     7681 agaaacagcg cccattggct gagctcagga atttgcggtg tttgctgcaa gatgtcgatg
     7741 atcggataac gatcctccat gtgcaccgcc atgaacagcc gtggcatcac cgggaagtga
     7801 aaagccatat ggcattcatc gccgctgccg aagtaagccg ccgcgtcctc aggccactga
     7861 ttggcttcgg cgagcagcat gcggtcgcga tagcgttcgt cgacatgccg gcgcaggact
     7921 ttcaaaaagc cgtgggtctc ggccagattt tcacagcttg ttccctcgcg ttcgaataga
     7981 tacggcacgg catcgagacg caaaccgtcg actcccagac ccaaccaaaa gtcgagagcc
     8041 gcgaaaatcg ccttctgcac cgctggattg tcgaagttaa gatccggttg gtggctataa
     8101 aagcgatgcc aatagtacgc cttggcgagc ggatcccagg accaattgga agtttcaaaa
     8161 tctttgaaaa taatccgcgc gtcctgatat ttctcctggg tctcgctcca cacgtagaaa
     8221 tcgcgcgcct tactccccgc cggcgcccgg cgcgcgcgct ggaaccacgg atgttgatcc
     8281 gaggtgtgat tgatcaccaa ctcggtgatc acgcgcaggc cgcggctatg cgccgccttg
     8341 aggaatgtcc gaaaatcgtt cagcgtgccg taacttggat ggatatcggt gtagtcggct
     8401 atgtcatagc cgtcgtcgcg cagcggcgac ggataaaacg gcaacagcca gagtgccgta
     8461 atgccgagat cttgcaaata atcgagcttt tccgtcagac ccttgaaatc accgatgcca
     8521 tcgccgttgc cgtcggaaaa ggcgcgcacg tggatttcgc agatgatcgc gtctttgtac
     8581 caacgtgcgc gctcggcgcg tgtcaatggc agcttttctt cgacagccgc ttgggctagc
     8641 gtttgcgatt tgtcactcat agatcacatg aagtaatcga actgctgctc gcgccgcaaa
     8701 tgacggcgca gacgaaaaac atgggccggc acgatcgccg gatcgagttc gagatagagc
     8761 cgcgagccat gccaaaagta gcggctgtcg cttatcaaat catgcagctg atagccgcgt
     8821 ttcccgtcga ctgccagttc gtcgatattg ggcgttgccc agcctgactg tttgtgatgc
     8881 gggtcgagat tgacggcgac gatgataata ttttccgagt cggcgctggt tttgctgtaa
     8941 caaagcagct gttcgttatc ggtcggatga aagctcaagc tgcggtcgtt atgcagcgcc
     9001 gtattctcgc ggcgaatacg attgatgcgc gtgataaaat ctttaaggct ggcgggatcg
     9061 tcaagattcc actgtcgcaa ctgatatttt tccgaatcga gatattcttc gctgcccggc
     9121 tcgcgcgccc gcgcctcgca caattcgaaa gccgggccat agatgccgta attggcagcg
     9181 agcgtagcgg ctaagactaa acgcgccata aatgccgggc gaccgccata ctggaggtac
     9241 tcggggagta tgtccggcgt gttaggccat aggttggcgc gaaaatattc gcgcgcgtca
     9301 ctcccggtaa tctcgctaaa ataatcggtc aactccgagc tcgtgttgcg ccaggtgaaa
     9361 taactatacg actggctaaa acccagttta gccaagcggc gcattacctt tggccgcgtg
     9421 aacgcctcgg caagaaatat tgcgtcggga taatcttgtt gcaacgccgc gatcaggtat
     9481 tcccaaaagc cgaagggctt ggtgtgcggg ttatcgacgc ggaaaatacg cacgccgcgc
     9541 tccagccaaa aacgcgcaat actctcgagc tcctgccaga gctcgcgcca atcttccgac
     9601 tcgaaattaa acggatagat atcctggtac tttttcggcg ggttttccgc gtattgaata
     9661 gtgccgtccg gtcggtgacc gaaccattcc ggatgagctg tcacatacgg atgatccggt
     9721 gaacattgat acgccaggtc gagcgcaatc tccaatccaa gttcgccggc cgttaccagc
     9781 aaacggtcga agtcttcgag ggtgccgagc tccggatgaa tcgccttgtg accgccctcg
     9841 gcggcgccga tcgcccatgg gctgccggga tcaccgggca tactgagaag tgagttgttt
     9901 ttacctttac gatggctgcg gccaatcgga tgcagcggcg ggagatagag cgtgtcgaac
     9961 cccatcgacg cgacgtaggg caagcgcgct gcgcaatcgg cgagcgttcc atgcgcgccg
    10021 gcgcggccgc aggagcgcgg gaacaactcg taccacgcac taaaacctgc cttctcgcgc
    10081 tcgacgagga ccacgagatc acgttcgtac tgggttgcgt agcgcaggtc gggatagcgc
    10141 gccatgagct gtccgagctc gtctgcttgc gtgccggcga gctcccaggc ggcatcatca
    10201 cggcgacgca tcagctcagc ccaacgccgc agcgcgttgg ctgcgttttt atcagcccgc
    10261 gccgcagcgc catcgatcaa cagggcgccg atcaataatt cgaccgggtc ctgctggcca
    10321 tcggcgattt tttttgccag accgcggcgc caggtggcga aaacatcgag ccatccgcgg
    10381 atggaataat aatagcggcc caattcgccg acgcgaaacc aggcgcgcca acgatcgttg
    10441 gcgcgcggct ccatgggact ctccgaccag ttggtttgcg aactatggcg ggtcaatagc
    10501 gtcgctgcga cttgatcatg gccgtcgaca aacacatcgg cctcgacgca gacgctttcg
    10561 ccgacggtgc gcttggcggg aaagcgaccg ccatcgatct ccggcttgac gccttctatg
    10621 ataactcgcc gcctatccat catgggacga ttccgagggc gctctcttcg atctgcggga
    10681 atgtcagggg gccatcggcg ctcgcttcgg cctgcagcaa ggccactgga aagcgcgcga
    10741 aaatttcgct cagcgacagc gcctggcctt tcgcggtcat gtgcgcccgc acggtctcgc
    10801 cggtgaaaac attggaccag cgcaagcccg ccagaggcgg cagcaggagt tcgttgccgg
    10861 cccacaaatc tctcaactcc gctaaacttg cgccatactc tgcaagcaac cgcggcacca
    10921 ctgcgacgac ccattgagaa tcgtagcagc gcgcgaacgc acaaacatgc tctgtcatct
    10981 cccccgccac cgccagcggc aagtattctc cctggagaaa tacccgcgcc tggttacggc
    11041 gcagctgcag tcccttgtag acaacgtaga gtttgatcgc gccgtcgcgc ggactgtcga
    11101 gcaggcgatc gagaagtgcc tgcgggttgt tgcgttcgag ctccatgagg tcggccagga
    11161 ttctccgccg cagcgagaaa tccaccgggc ggcggttgtc cggatcgaca aaactgaaat
    11221 cccacagttc ggttccctgg taaaaatccg gcacaccggg agcggcgatc ttgagaacag
    11281 tctgtgctag tgaattgatc gcgccgtgaa atgcca
//
LOCUS       DATLCH010000087         7403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8336138, whole genome shotgun sequence.
ACCESSION   DATLCH010000087 DATLCH010000000
VERSION     DATLCH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8336138"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VMZ02_02555"
     CDS             complement(<1..632)
                     /locus_tag="VMZ02_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR70837.1"
                     /translation="MNNSDLIVRTLEQAGVRWVFGIPSGPVLPLIEALRQSSVAFVLT
                     ATETSAAFMASAVGYLTGVPGVCVSTLGPGATNLTTGVGSAWLDRAPLLAITCNIATT
                     WLRRRIQMRIDHHKLFEPLTKASLACRQGNVGEMFAQAIGIAIQEPPGPVHLDLPEDV
                     AQALALEARSSRITPTMAADLSTDAATAVAAALNSAKRPLLITGLTFTRSA"
     gene            complement(720..1526)
                     /locus_tag="VMZ02_02560"
     CDS             complement(720..1526)
                     /locus_tag="VMZ02_02560"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HUR70838.1"
                     /translation="MAVKSIGIVGCGAIGKALVRAVEAGKLAVRIAGVTSRSPDSARE
                     FLAGFRNPPSYLPLSQLIEASDLLVEAAGGHVVVELARSTFAAGKDLMVISVGALLEN
                     PEVMAESRRTGCRLYVPSGAIAGLDGIKSACVGAVSHVTHTTRKPPLGLEGAPYLVER
                     GISLVGLQEETEVFSGSARDACRGFPANVNVTGAVSLAGIGPDKTHVRILAVPGLPRN
                     CHDIDVEGEFGKLHVHIENIPSENPKTGKLTAMSIVRALQDVIDPVRIGN"
     gene            complement(2098..2568)
                     /locus_tag="VMZ02_02565"
     CDS             complement(2098..2568)
                     /locus_tag="VMZ02_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HUR70839.1"
                     /translation="MRFPELDVAAGYFPAVMTAAQLPTLNAALNSLSAVLLCAGYFFI
                     RSQNRAAHQRCMVAALTCSTLFLISYLTYHFQVGSIGFKGRGWIRPIYFTILITHTIL
                     AAAVVPLALVTVIRALRQRFDAHRRIARWTFPIWLYVSITGVVIYLMLYKISWS"
     gene            complement(2520..2798)
                     /locus_tag="VMZ02_02570"
     CDS             complement(2520..2798)
                     /locus_tag="VMZ02_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HUR70840.1"
                     /translation="MSSTAHSHHNYIKIFIILSVLTAVEIGITFLGLPRKLLVSLLVG
                     LAVWKAALVALHFMHLKLEKKTLTIIAIVPFVLCVFLSLMLLPDIFPR"
     gene            complement(2821..3429)
                     /locus_tag="VMZ02_02575"
     CDS             complement(2821..3429)
                     /locus_tag="VMZ02_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HUR70841.1"
                     /translation="MSEAVAVHGAVEPAETPLTPESWGKLGMWLFLAGDAMSFGGLIV
                     AYGALRYHSKSWPVPAEVLGINLTALMTFLLIVSSVTMVLALAGLQEGDKRRFNKFMS
                     FTILGGALFLGLQAYEWTHLIGHTLPHYNLAFGKDLFATTFFVLTGFHGMHVTGGVIY
                     NSSVLAAVNRGRYQAKHVEIAGLYWHFVDLVWILIFTFVYLL"
     gene            complement(3426..3665)
                     /locus_tag="VMZ02_02580"
     CDS             complement(3426..3665)
                     /locus_tag="VMZ02_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70842.1"
                     /translation="MILPSFARACSVCFSGDDDAVTHAFNWSVGFLLAAPYVITGTIA
                     GCLAVAYRRAAAKRATLEEQETSDSLIWTQEESAK"
     gene            complement(3762..4427)
                     /locus_tag="VMZ02_02585"
     CDS             complement(3762..4427)
                     /locus_tag="VMZ02_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HUR70843.1"
                     /translation="MAAGSLARKLDDTAPEIDAKRRRESSGGGGGPPVRPNVPLLSNA
                     RLAVVMLLGAELMFFAGLIGAFLVFRLSAQVWPPPFQPRLPLGVTGVNTAILLFSALT
                     VHWAVGAARAGAIARVVRHLTWTALLGALFLVIQGYEWLRMIHFGLTTSSSVYGGLFY
                     TLIGAHGVHVIGGMIWLLFVRWQSKRGRFTSKNFVGIQTTRMYWTFVVALWPVLYGLV
                     YLY"
     gene            complement(4571..5476)
                     /gene="cyoE"
                     /locus_tag="VMZ02_02590"
     CDS             complement(4571..5476)
                     /gene="cyoE"
                     /locus_tag="VMZ02_02590"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HUR70844.1"
                     /translation="MSSNTDTVILSGTSTLRRAGDFVELAKPRVVLMVLITTFVGFYL
                     GSEQVPQYLILLQALIGTALAAGGTLALNQYMERDSDAVMQRTRRRPLPDGRLQPREA
                     IWFGIAVTILGLGYLALAVNLMSAYVTAFITISYLLFYTPLKRRSSLCMLVGAIPGAL
                     PPVIGWVATRDSLDIGAWVLFAIMFLWQVPHTLAIARLYREDYAKAGIMFLPVIEPEG
                     SSSNRQIVSHSAALLAVSLLPTLLGIAGGVYFVVAFTLGVGFIAAGITLALNSTLANA
                     RRLLFASLVYLPVLLLVMALDRIPL"
     gene            complement(5473..6438)
                     /locus_tag="VMZ02_02595"
     CDS             complement(5473..6438)
                     /locus_tag="VMZ02_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HUR70845.1"
                     /translation="MKPDPRRMAAVSAGHPSSFSPWPHRVALGLAWATFPLLFIGGLV
                     TSLGAGLAVPDWPTTFGYNMFLYPWSRMIGGIFYEHSHRLIASCVGLLSIVLAVTFWI
                     KEDRRWLRWLGIGALGMVILQGVLGGLRVILLQHTLAIIHACFAQAFFALTVSLALFT
                     SQEWRAQKPPSIDDGGRLRRLASITTALVYIQIVFGALLRHTGERLDGHLLFAALVAI
                     HVVLLLLRISNHHGAELVLVRPAYWLGGLLLVQLSLGTAAYLGKFTALLPLGGGALVL
                     TATTHLIVGALMLATSLTITLRAFHHSHRRRSRQDRTVLKQQYSL"
     gene            complement(6454..>7403)
                     /locus_tag="VMZ02_02600"
     CDS             complement(6454..>7403)
                     /locus_tag="VMZ02_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HUR70846.1"
                     /translation="QHMFWFFGHPEVYILVLPAMGITSDLLSTFSRKPIFGYHAMAFS
                     MIAIAFLSWIVWGHHMFISGMNPLLGTAFMMTTMVIAVPSAIKVFNWLGTLWGGSIRL
                     TVPMLFALGFVSNFLIGGLSGIFMASTPVDIFIHDTYYIVAHFHYVVAGIIFALFAGI
                     YYWFPKMFYRQMNQTLGKIHFVLTYIFFNGAFFPMHFLGVGGHMRRIYNPTQYEFLKP
                     LQDWNVFITWSALILGASQIFFLWNFCQSLIAGKKAERNPWQANTLEWSAPTPPPHGN
                     FETTPTVYRGAYEYSSPEVKEDWLPQDKNIGSGAAVRAH"
BASE COUNT         1935 a         2100 c         2022 g         1346 t
ORIGIN      
        1 gcacttcgag taaaggtcaa cccggtaatt aacagtggcc gcttagcact gtttagcgca
       61 gcggcaaccg ccgtagcggc gtccgtcgac aagtccgcag ccatggtcgg cgttattcgt
      121 gagctgcgag cttccagcgc caatgcttga gcgacatcct cgggcaagtc gagatgcacc
      181 ggccccggcg gttcttggat cgcgatgccg atcgcctgag cgaacatctc gccgacattt
      241 ccctgccggc aggcgagcga tgccttggtt agcggctcga aaagcttgtg atgatcgata
      301 cgcatttgga tgcgccgtct gagccaggtc gttgctatat tgcaggtgat tgctaacagc
      361 ggcgcacgat ccaaccatgc cgagccgacg ccggtggtaa gattggtcgc ccctggcccc
      421 agcgtggaaa cacacacacc gggaactccg gtcaaataac ccaccgcgga agccatgaac
      481 gccgccgacg tctcggtcgc agtgagcacg aaagcaaccg agctctggcg cagcgcctca
      541 atcaacggca ataccggccc gctcgggatg ccaaacaccc agcgcactcc ggcctgttca
      601 agcgtgcgaa cgatgagatc gctgttgttc atggaatggg gtggcgttga gggattatag
      661 gcgatcgagg gagcagatcg cagtgtatcg ctcgttttgg cgttttcgcc ggcgatcaat
      721 cagttaccga tccgcaccgg atcgatgaca tcttgcagcg cgcgcacgat cgacatcgct
      781 gttagcttcc cggttttggg attttcgctg ggaatgttct cgatatgcac gtgcagcttg
      841 ccgaattcac cttcgacatc tatgtcatga cagttacgcg gcaagccggg caccgcgagg
      901 atgcgcacgt gtgtcttgtc gggaccgatg cccgccaaac tcacagcgcc ggtcacgttg
      961 acattagcgg gaaaaccacg acaagcatct cgcgcggatc cggagaacac ctctgtctcc
     1021 tcctgcaaac cgacaagcga gatgccgcgc tcgaccaaat acggcgcacc ctcaaggccc
     1081 aagggcggtt tgcgcgtcgt atgggttaca tgcgaaaccg caccgacaca ggcagattta
     1141 ataccgtcga gaccggcaat agcaccggac ggaacataga gacggcatcc tgtgcgccgt
     1201 gactctgcca tcacctcagg gttttcaagt agggcgccga cactgatgac cataagatct
     1261 ttgccggcag cgaaagtgct gcgcgcgagc tctacaacca cgtggccgcc cgccgcttcg
     1321 acgagcagat cggaagcttc gatcagctga ctcaaaggca gataagacgg tggattccta
     1381 aagcccgcga gaaactcgcg cgcgctatcg ggtgagcggc tggtcacgcc ggcaatgcgc
     1441 accgcgagct tgcccgcttc caccgcgcgc accagcgctt tgccaatcgc gccgcatccg
     1501 acgataccta tcgatttgac tgccatcgtg agctgccacc cttcacaaac aaaaatatga
     1561 atctatctag ctcgaagcaa ttctccctgt caatcgagga aaatcaacga gacaattcat
     1621 gcaaatatac gccgtcgtga caaagtgaaa gacattctag cagatctcca aagatgttgg
     1681 accgaggcaa ccaattgcaa gcgactgccg caataaaaat actcgggtta atcttggaaa
     1741 atcgtcagcg ggatggtaca cgagacctat gccggcttac gagatctatt tccgttgtga
     1801 ggattgcaaa cgcgagcata ccatccacac caagattcac ctaagccagg ggcctgatcg
     1861 taaacaaagc ctggccgaat tctttgttgg gcgttcgatg ccaccgcagg cgtcatcgct
     1921 gcagggccat aaggccttct gcctaagaac cggacggcaa tttcgactgg aaagcgatga
     1981 gcaaatcctt ctggtaccgc cctcagcccg tccgcttccc ccgagccaac tgccgctgat
     2041 tcccgacagc cattgactcg cctcgaagac ggctatgact gcggcggtgt tagcggctta
     2101 agaccaacta attttgtaca gcatcagata aatcaccacg ccggtaatcg aaacatacag
     2161 ccagatagga aaagtccagc gcgcgattcg gcgatgagcg tcgaatcgtt ggcgtagcgc
     2221 gcggatcacc gttaccagcg cgagtggtac gacagcagcc gcaagaatag tgtgggtgat
     2281 gagaatcgtg aagtaaatcg gacggatcca gcctcgccct ttaaagccta tggagcccac
     2341 ctggaagtgg taggtcagat aggaaatcaa aaataatgtc gaacaagtga gggccgccac
     2401 catgcaccgc tggtgggcag cacggttctg gctgcgaatg aaaaaataac cggcacaaag
     2461 caaaacggcg ctgagggaat tcagcgccgc attcagagtc ggaagctgtg cagcggtcat
     2521 caccgcggga aaatatccgg cagcaacatc aagctcagga aaacgcacag cacgaacggc
     2581 acgatcgcga tgatagtgag tgtcttcttt tcgagtttga gatgcatgaa atgcagcgct
     2641 acgagtgccg ccttccacac tgccaagccg accagcagtg acaccagcag cttcctgggt
     2701 agccccagga aggtaatgcc aatctcaacg gcggtcagca ccgagagaat aataaatatc
     2761 ttgatgtagt tgtgatgcga gtgggcggtt gatgacatgg gcagaccctc gagcggtgga
     2821 ttataagaga taaacaaagg tgaagatcaa tatccagact agatcgacga agtgccaata
     2881 aaggccggcg atttcgacat gcttggcctg atacctgccg cgattgaccg ccgcgagcac
     2941 gctggagtta tagatcacgc cacccgtgac atgcatgcca tgaaaaccgg tgagcacgaa
     3001 gaaagttgtc gcgaacagat ccttgccgaa cgccaaattg tagtgcggca gcgtgtggcc
     3061 gatgagatgc gtccactcat aggcttgcag gccgagaaaa agtgccccgc caagaatcgt
     3121 gaagctcatg aatttgttga accgtcgctt atcgccctcc tgcaaacccg ctaaagccaa
     3181 caccatggta acgctgctga cgatcagcag gaacgtcatc aatgcggtca ggttgatgcc
     3241 caacacctcg gccggcacag gccagctttt gctatggtaa cgcaaggcac cgtaggcgac
     3301 gattagaccg ccgaacgaca tcgcatcgcc ggcgagaaat aaccacatgc cgagtttgcc
     3361 ccagctctcc ggggtcaacg gggtttcagc tggctcaacc gcaccatgca ccgctaccgc
     3421 ttcactcatt tcgcgctttc ctcctgagtc cagatcagcg aatcactggt ctcctgttcc
     3481 tccaaagttg cgcgcttcgc cgcggccctg cgataggcga ccgcgagaca gccggctatc
     3541 gtcccggtaa tcacgtaagg tgcggcgagc aagaagccga cgctccaatt gaaggcatga
     3601 gtcactgcgt catcgtcgcc ggaaaagcac accgaacacg cccgtgcaaa cgacggcaag
     3661 atcattgcaa cgaagggaat agcaattaca actcttgtcc tgaagaacat aaacagccga
     3721 aaaactatct taacgactcc gaactgtcaa ctaaaccgcg ctcagtaaag ataaaccaag
     3781 ccataaagca cgggccacaa cgccacgaca aaggtccagt acatgcgcgt ggtttgtatg
     3841 ccgacgaaat tcttactggt aaatcgtcct cgcttggact gccagcggac aaacaatagc
     3901 caaatcatgc cgccgatgac gtgaacacca tgcgcaccga tgagcgtgta gaatagcccg
     3961 ccataaacgc tgctcgatgt ggtcaagccg aaatgaatca tacgcaacca ctcgtaaccc
     4021 tggatcacta aaaacagcgc gcccaataga gcagtccagg tcaagtggcg aacgactcga
     4081 gcgatcgcgc cggcgcgcgc ggcgccgact gcccaatgaa ccgtcaaggc gctgaacagc
     4141 aatattgccg tgttgacgcc ggtgacaccc agcggcaagc gtggctgaaa cggcggaggc
     4201 cagacctggg cgctcaagcg aaacactaaa aatgcgccga tcagaccggc gaaaaacatc
     4261 agttcggcgc cgagcagcat gactacggca agacgggcat tgctgaggag cggcacattt
     4321 ggccgcacgg gaggaccgcc gccgccccca gaggattcac gccgacgttt ggcatcgatc
     4381 tccggtgcgg tgtcgtccag cttgcgagct aaagaaccgg cggccatgat ttttgcttca
     4441 aacgaacact aataaattat gataaacgct atgatcgcgg cgatgtagag aatcacggca
     4501 gccgccaaga gcgcgccgaa acggaaggca cgtgcaggct tttgtcgact aggttcggtt
     4561 aagatctggc tcataagggt atgcggtcca gagccattac cagcaagagc actggcaaat
     4621 ataccaacga tgcgaacaac agccggcgcg cgttggccaa ggttgaattc aatgccaatg
     4681 ttatgccggc ggcgataaac ccaacgccca acgtaaaagc gacaacgaaa tagactcctc
     4741 cggcgatacc gagcaaagtt ggcaataaac tgacggccag cagcgcggcg ctgtgactga
     4801 cgatctggcg gttgctactg ctgccttcgg gttcgatgac aggcagaaac ataataccgg
     4861 ctttggcata atcttcacga tagagccgag caatcgccaa agtatgcggt acttgccaaa
     4921 gaaacatgat tgcgaataaa acccaggcgc caatatccaa tgaatctcgc gtcgccaccc
     4981 atccaataac cggcggcaat gccccgggaa tggcgccgac cagcatgcat aacgagctgc
     5041 gccgtttaag cggggtgtaa aataacaaat agctgattgt gataaacgca gtcacgtacg
     5101 cgctcatgag attcaccgct agtgccagat agccgagccc caatatcgtt acggcgatcc
     5161 cgaaccaaat cgcttcccgc ggttgcaaac gaccgtccgg cagcggccga cgccgggtcc
     5221 gctgcatgac cgcgtcgcta tcccgctcca tatattgatt gagcgctaag gtcccacccg
     5281 ctgccagtgc ggtgccgatc aaggcctgca gcaaaatcaa atactgcggt acctgctcgg
     5341 aaccgagata gaaaccaacg aacgtcgtaa tcagcaccat gagtacgaca cgcggcttag
     5401 ccaattcgac aaaatcacca gcccgccgca gcgtacttgt gccgctcaag atcacggtat
     5461 cggtgttcga gctcatagtg aatactgttg cttcaacacc gtccggtcct ggcgtgatcg
     5521 gcgccggtgc gagtgatgaa acgcccgcag agtgatcgtc aagctggtgg ctagcatcaa
     5581 cgcaccgact atcaaatgtg ttgttgcggt caagaccaag gctccgccgc ccagtggcaa
     5641 cagagcggta aatttcccta gataagcggc ggtaccgagc gacaactgga cgagcaacag
     5701 cccgccgagc cagtaagccg gccggaccag caccagttct gcgccatgat gattgctgat
     5761 acggagcagt aacaaaacca catgaatcgc gaccagcgcg gcaaacagta aatggccatc
     5821 gagccgctcg cccgtgtgtc gcagcagcgc gccgaaaacg atctggatat agacgagggc
     5881 cgtggtaatc gaggcgagcc ggcgcagccg gccgccgtca tcgatcgatg gaggtttttg
     5941 agcgcgccac tcttgcgagg tgaagagcgc cagactgacg gtcaatgcga aaaaagcttg
     6001 agcgaaacag gcatggatta tcgcgagggt atgttgcagc aagatcactc gcagcccgcc
     6061 gagcacgcct tgcaaaatga ccatgccgag cgcgccgatg ccgagccagc gcagccaacg
     6121 gcgatcctct ttgatccaga acgtcacggc aagaacgatg ctcaagagac cgacacacga
     6181 tgcgatcaga cgatgactgt gttcgtaaaa aatgccgccg atcatcctcg accaggggta
     6241 gagaaacata ttgtagccga aggtcgtcgg ccaatccggc acggccaatc cggcgcccag
     6301 acttgtcacc aaaccaccga tgaagagtag cggaaaagtc gcccaggcca agcctagcgc
     6361 gactcggtgc ggccagggcg aaaatgatga aggatggccg gcagagactg ccgccatcct
     6421 tcgagggtcc ggtttcattg taccgagttt cgatcagtgc gcgcgtaccg cggcgccgct
     6481 gccgatgttt ttatcttggg gcagccagtc ttctttgact tcgggcgaac tgtattcata
     6541 agcgccgcga tagaccgtgg gagtggtttc aaaattacca tgcggcggcg gcgttggtgc
     6601 cgaccactcc agggtattgg cctgccacgg attgcgctcg gccttcttcc cagcgatcaa
     6661 actctggcag aaattccata aaaagaaaat ctgcgaggcg ccgagtatga gcgcgctcca
     6721 agtgatgaaa acgttccagt cctgcagcgg tttcaagaat tcatactgtg ttgggttgta
     6781 gatacgccgc atgtggccgc cgacaccgag gaaatgcatc ggaaagaacg cgccgttgaa
     6841 gaaaatgtag gtcagcacga aatgaatctt gcctaaggtc tgattcattt ggcggtaaaa
     6901 cattttagga aaccaataat aaataccggc aaacaaagcg aagatgatac cggcaacgac
     6961 gtaatgaaag tgcgcgacaa tgtaataagt atcgtgaata aagatatcga ccggcgtcga
     7021 cgccatgaag atgccgctca gcccgccgat caaaaagttc gatacgaaac ccaatgcaaa
     7081 cagcatcggc acggtcagcc gaatcgaacc gccccacaga gtacccagcc agttaaatac
     7141 tttaatcgcc gagggcacgg cgatgaccat agtcgtcatc ataaatgcgg taccgagcag
     7201 cggattcata ccgctgataa acatatgatg cccccagacg atccacgata ggaatgcgat
     7261 tgcgatcatg gaaaaagcca tcgcatggta gccaaagatc ggtttgcgcg aaaaagtcga
     7321 cagcaaatcg gaagtgatgc ccattgcagg aaggaccagg atgtaaacct ccggatgacc
     7381 aaagaaccag aacatatgct gcc
//
LOCUS       DATLCH010000088         5311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_280588, whole genome shotgun sequence.
ACCESSION   DATLCH010000088 DATLCH010000000
VERSION     DATLCH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280588"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..1419
                     /locus_tag="VMZ02_02605"
     CDS             361..1419
                     /locus_tag="VMZ02_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HUR70847.1"
                     /translation="MESLEVLVMQQVPDAVLGRITQVDPRINVIDARGWFDVELRATW
                     PQWTVNRYLGDRKYPATTLEQRNQSLAAAEIMLTGWPPVKDIRARAPRLKWLHQLPAG
                     ASNLVGTDLWASNVVVTTSRGLTNRRPMAEYVLASFLHFSRGLHLSYRDKQRHQFDHR
                     SYDPITIRGKTVCVVGAGGIGEEVANLCAAAGMRVVGTRRKVSAKSVIPPAFARLEPS
                     EAMHDLLRESDFVAICCPWTKETHHLIGADALAAMKPGTVLVNIARGEIIDEEALIAA
                     LGQNKLRGFATDVYDGEFERPPDVRLWQDERVVITPHVSAMADVSEHQGVQLFCDNLR
                     RYLDGQPLVNVIDWQRGY"
     gene            complement(1684..2430)
                     /locus_tag="VMZ02_02610"
     CDS             complement(1684..2430)
                     /locus_tag="VMZ02_02610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HUR70848.1"
                     /translation="MRFKDKSVIITGGGGKIAKAYALAFAKEGAKLSLPDIASADQIV
                     KTVKDMGGTAISMACDVSNESSVRKMVDETVKQFGTVDILINNAAYFMSVWKGPFWEM
                     SVEEFDKAMAVNVRGSWLCAKAVAPLMQKQKSGKIINISSNVALTGNPNYIHYVTSKG
                     ALIAMTRAMAKELGDWNICVNSVSPGFVVTEGRQVDPEYEKIRAQQRSLKKTQVESDL
                     VGTVLFLSSSESDFMTGQLLNVDGGFHFVG"
     gene            complement(2460..3206)
                     /locus_tag="VMZ02_02615"
     CDS             complement(2460..3206)
                     /locus_tag="VMZ02_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR70849.1"
                     /translation="MRFQGKSVIITGGGGKIGKAYAMGFAKEGAKVALPDIASADHVV
                     KAIQDMGGTAISMACDVSDEKSVKAMVDSVAKQFGSVDVLVNNAAYFMTVKKSPFWEM
                     EVDEFDKAMAVNVRGSWLCAKAAFPYMKQQGKGKIINISSGTALNGGQNYIHYVTSKG
                     ALIAMTRAMAREVGDHGICVNTVSPGFVVTEGRAVDPAFNASRVGNRAIKRIQVENDL
                     VGTVMFLASPESDFMTGQLLNVDGGAHFVG"
     gene            3437..4396
                     /locus_tag="VMZ02_02620"
     CDS             3437..4396
                     /locus_tag="VMZ02_02620"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70850.1"
                     /translation="MAKLKLTIAFDKYDYLQPLREGKIEAQGLDLNIITVESGIRHER
                     MFHYGEYDACEFSMSSYLVARGKDIDRLQAIPFFPRRMWGHKFCFIKAGSGIKKPADL
                     KGGRIGLRSYENTLALVAKGMLMNSYDLGVSDVTWVIVNKEHVGFNPPPHIKVEFIDG
                     NRKLEDLLVEGKVDAEVEPDLPQRWIRGEGSVERLFPEFEKEERAYYKKTGIFPIMHP
                     VVIKKEILERDPWIATSLYEALLASRKAYNEFMEQPHRLSFAWGRSYLEEERQFFGKD
                     PFYQGMKENYNDVENLIKFAGQQGMLGRNLSVEELFTENTRTT"
     gene            4550..5278
                     /locus_tag="VMZ02_02625"
     CDS             4550..5278
                     /locus_tag="VMZ02_02625"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70851.1"
                     /translation="MSDYRIIDADGHVMELDSELREFIGAPYNDLEWHHSYSFWPGLT
                     MDGFLRSLRKKGGWAGGGTGPNAQDWLRFLDINKIELTVLYPTQGLTHAAIQDHDWAI
                     CMARAYNDWLYNKFMAVSPRLVGVALLPIQDIGEAVKELRRAVNDLGMVGAVLPAVAP
                     GGRLYSGPEFYPLWEEAQKLDVPVSTHGGLSMPALGLDSVNNFTIGHTLEHPFAQIRQ
                     FTALMFECCRFRISAKPSKSCAAR"
BASE COUNT         1206 a         1486 c         1437 g         1182 t
ORIGIN      
        1 agcctgcgca ttgatttacc tactcgcgcg ccgctgcgac ctcagcgatg ctcagttcag
       61 caagcactga actactctag gctgcaagcg gacgggttct caatttgacg actggtagtc
      121 aaattctccg tgatttccac gcaggcggga atcttaaact cttttgtgtt caatttcgga
      181 atgaacctgg atgccggccg gagtttatcc tgagcccggt cgaagggcgg catgacggaa
      241 tttcattcgc ccgaagaatg gctgcgccct ggcgaatgga ttagggattt caaccagcgt
      301 gatagatatt gaacctgaag ttacgaacaa aaacttatcg tggtaaggat catctcaact
      361 atggagagtc tcgaagtgtt ggttatgcag caggttccgg atgctgttct cggtcgcatt
      421 acccaggtcg acccacgcat caatgtcatc gatgccagag gatggttcga tgttgagctg
      481 cgcgctactt ggccgcagtg gaccgtcaat cgctatctcg gcgaccgaaa gtatccagcg
      541 acgacgctcg agcagcgcaa tcaatcgctg gcggcggcag aaataatgct caccggctgg
      601 ccgccggtca aagacattcg cgctcgtgcg ccgcggctga aatggcttca tcagcttcct
      661 gcgggagcga gtaatttagt aggaaccgat ctttgggcgt cgaacgtcgt cgtaaccact
      721 tcgcgcggtc tgaccaatcg ccggccgatg gcggaatatg ttttggcaag cttcctccac
      781 ttcagccgtg ggctgcatct aagctaccgc gacaaacagc gccaccagtt cgatcatcgg
      841 agctatgatc cgatcacgat ccgcggtaaa actgtttgcg ttgtcggcgc cggcggtatc
      901 ggcgaggaag tggcaaatct ctgcgccgcc gccggcatgc gcgtggtcgg cacgcggcga
      961 aaagtttctg cgaaatctgt aataccaccc gccttcgcca gattagaacc ttcagaagcg
     1021 atgcacgatc tgctgcgcga gagcgatttc gtcgcaatct gttgtccctg gacaaaggag
     1081 acgcatcatc tgatcggcgc tgacgcctta gcagcgatga aaccgggaac ggtgttagtc
     1141 aatatcgccc gcggtgagat aatcgacgaa gaagcgctaa tcgcggcgtt agggcaaaat
     1201 aaactgcgcg gctttgccac cgacgtttac gacggcgaat ttgagcgacc gcccgacgtc
     1261 cgcctctggc aagatgaacg cgtcgtgatc acgccgcatg tctcggcaat ggccgacgtc
     1321 tccgaacatc aaggcgtgca actattttgc gacaacctgc gccgctattt ggacggccag
     1381 ccgctagtga atgtcatcga ctggcagcgc ggttactaat actcatcgtc acgccggtgc
     1441 gaagcggcag ggtgaatgac cggactgctc gcttcgatcc tctcttccgt cattccggcg
     1501 cacgccggaa tctttgattt aaggtaatgc tgtgcttctc gggaatggat ggaactcttt
     1561 ttcgcaacaa aaatatttat caaacgaggg ggcatccacc ttcggttgcg tgtagaccga
     1621 gaaaccctgg attccggctt gcgccggaat gacgaatgag aaaaaacacg ttcgattaaa
     1681 gagttagccg acgaaatgaa agccgccatc gacatttaat aactggccgg tcatgaaatc
     1741 gctctcgctc gacgagagga acagtacggt tccgaccaaa tcgctctcaa cctgagtctt
     1801 tttcaaactg cgttgttggg cgcgaatctt ctcgtattcc ggatccacct gtcggccttc
     1861 ggtaacgacg aagccgggac tgaccgagtt gacgcagatg ttccaatcgc ccaactcctt
     1921 cgccatcgcc cgggtcatgg caatcagcgc gccttttgat gtcacgtaat gaatgtaatt
     1981 gggattgccg gtaagcgcga cattggacga aatgttgatg atcttgccgc tcttttgctt
     2041 ctgcataagc ggcgcgacgg ctttggcgca gagccacgaa ccgcgcacat tgaccgccat
     2101 cgctttgtcg aactcctcga cgctcatctc ccagaaaggc cccttccaca ccgacatgaa
     2161 gtacgcggcg ttgttgatga gaatatcgac cgtgccgaac tgcttgacgg tttcatcgac
     2221 catcttcctg acgctcgatt cattagacac gtcgcaggcc atgctgatcg ccgtgccgcc
     2281 catgtctttc actgtcttga cgatctgatc tgcgctggcg atatccggca agctcaactt
     2341 cgccccttct ttagcgaacg ccaaagcata cgctttggca atcttgccgc cgccccccgt
     2401 gatgatgacg gacttatctt tgaaacgcat gtgaacccct gaaatagatc gtcctgtttt
     2461 cagccgacaa aatgggcgcc gccatcgacg ttcaacaact ggccggtcat aaaatcgctt
     2521 tccggcgacg ccagaaacat gacggtgccg accagatcgt tctcgacctg aatgcgtttt
     2581 attgcgcgat tgccgactcg gctggcattg aacgccggat cgaccgctcg tccctccgta
     2641 acgacaaatc cagggctgac ggtattgacg cagataccgt gatcgccgac ctcgcgggcc
     2701 atcgcccggg tcatggcgat cagcgcgcct ttggatgtga cgtaatgaat atagttctgc
     2761 ccgccgttga gcgcagtgcc ggaggatata ttgatgatct tgcccttccc ttgctgcttc
     2821 atatacggga acgccgcctt ggcgcacagc cacgagccgc gcacgttgac agccatggct
     2881 ttatcgaact catcaacttc catttcccag aacgggctct tcttcacggt catgaaatat
     2941 gccgcgttgt tgaccagaac gtcgacgctg ccaaattgtt tggcaacgct gtcgaccatc
     3001 gccttgacgc tcttttcatc cgacacatcg caagccatgc tgattgccgt gccgcccatg
     3061 tcctggatcg ctttgacgac atgatcggcg ctggcgatat cgggcagcgc cacttttgcg
     3121 ccttctttgg cgaaacccat cgcataagct ttgccaatct tgccgccacc gcccgtgata
     3181 attaccgact tgccttggaa ccgcataaag ttctccttta gcttaactta agtgtcactt
     3241 aacatcggct ccccgagggt gtcaacgaaa tcagctgatt gacagcacca cagtcgactt
     3301 ttatgatccg ccaagcaacg aagcggaatt gaacccgcag ccatttgtcg atatggaatg
     3361 agtcagagaa caggagagcg acggtttcaa ttaactgaat cttcatcaag aaattttaaa
     3421 agccgaggcg acattcatgg ctaaactgaa actcaccatc gcattcgaca aatacgacta
     3481 cctgcaacca ttgcgcgagg gcaagatcga agcccaaggc ctcgacttga atatcatcac
     3541 ggtggaaagc ggcatccgcc atgagcgcat gtttcactac ggcgagtacg acgcctgcga
     3601 gttctccatg tcgtcctacc tggtcgcccg cggcaaagac atcgaccggc tgcaagcgat
     3661 cccgttcttc cctcggcgca tgtggggaca taaattttgc ttcatcaaag ccggctccgg
     3721 catcaagaag ccggccgatc tcaaaggcgg ccgcatcggg ctgcgcagtt acgagaatac
     3781 tctggcattg gtcgccaaag gcatgttgat gaacagctat gatctcggtg tcagcgacgt
     3841 tacctgggtg atcgtcaaca aggaacatgt cggcttcaat ccgccgccgc atatcaaagt
     3901 cgaattcatc gacggcaatc gcaagctcga agatctgttg gtcgaaggca aggtcgacgc
     3961 tgaagtcgaa cccgacctgc cgcagcgctg gatccgcggc gaaggctccg tcgaacggct
     4021 gtttccggag ttcgagaaag aagagcgcgc gtattacaaa aaaaccggca tctttccgat
     4081 catgcatccg gtggtcatca agaaggaaat cctcgagcgc gatccatgga tcgcgacaag
     4141 cttatatgaa gcactgctgg catcgcgcaa ggcctacaat gaattcatgg aacagccgca
     4201 ccggctgagc ttcgcgtggg gccgctccta tctggaagaa gagcgccagt tttttggcaa
     4261 ggatccgttt taccaaggga tgaaagaaaa ctacaacgac gtcgagaatt tgattaagtt
     4321 cgccgggcag cagggcatgc tcgggcggaa tttgagcgtc gaggaattgt ttaccgagaa
     4381 tacgagaacg acgtagcgtc gattttgata tgtttgaaat accaggcctt tagtcaaatt
     4441 gaagttttgt cattccggca aaagccggca tgcatctgca ctacttgtgc tgcaagggca
     4501 aaaaaaactg gattccggcc tgcgccggaa tgacgaacgg gaggacacta tgtctgacta
     4561 ccgaatcatc gatgccgacg gacatgtgat ggagctcgat tccgaattgc gcgagtttat
     4621 cggcgcgccg tacaacgatc ttgaatggca tcacagctac tcgttctggc ccggtctcac
     4681 aatggacggc tttttgcgct cgttgcgcaa aaaaggcggc tgggccggcg gcggcacagg
     4741 ccccaatgcg caagactggc tgcgcttcct cgatatcaac aagatcgagt tgacggttct
     4801 ctatccgact caaggtttga cccatgccgc gatccaagat cacgactggg cgatctgcat
     4861 ggccagagct tacaacgatt ggctttacaa caagttcatg gcggttagcc cgcgcctggt
     4921 cggcgtcgcg ctgctgccga ttcaggatat cggcgaagcc gtcaaagagc tgcgccgcgc
     4981 ggtgaatgac ctgggcatgg tcggcgcggt gttgccggct gtagctcccg gcggccggct
     5041 ctacagcggt ccggagtttt atccgctctg ggaagaagcg cagaaactcg acgttccggt
     5101 gtcaacccac ggtggcctga gtatgccggc gctcggcctg gattccgtca acaattttac
     5161 gatcggccat acgctggaac atccgttcgc gcagattcgc caattcaccg cattgatgtt
     5221 cgaatgctgc cgattcagga tatcggcgaa gccgtcaaag agctgcgccg cgcggtgaat
     5281 gacctgggca tggtcggcgc ggtgttgccg g
//
LOCUS       DATLCH010000089         6215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_282473, whole genome shotgun sequence.
ACCESSION   DATLCH010000089 DATLCH010000000
VERSION     DATLCH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282473"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..344)
                     /locus_tag="VMZ02_02630"
     CDS             complement(147..344)
                     /locus_tag="VMZ02_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70852.1"
                     /translation="MKAMETDLNKPSYWQLVFLQSKVNGAAYFGEELPGRNWKWPKDT
                     YLQRRLQRDKWTQATRSSNLN"
     gene            complement(741..1736)
                     /locus_tag="VMZ02_02635"
     CDS             complement(741..1736)
                     /locus_tag="VMZ02_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70853.1"
                     /translation="MPLLTIDLGCGDYDIVRPLKDGAVRAEGLEINFITINRPPEVHW
                     RMGMHQEFDAAEMSFGSFVAGKARGNYPFIGIPAFVYRKFRHSCAYVNSTAGIDKPTD
                     LKGKRVGIPEWQMTATVWLRGFLQDDYGVRPSDMHWLTGGLESSERKEKVALKLPPEI
                     DIENIPTGKNLSDMLVAGEIDALLTAQVPAPFVKRAPQVRRLFADPRAVETDHFRRTG
                     IFPIMHVMVLREQLFQRHPWIAQSLYKALLDAKQHCVDTIFRNDAIHSVIPWAANHAE
                     EVRQLMGQDFWPYGVEANRKTLETFLRYSIEQGVASRNLGVDELFPRATLDTFKV"
     gene            complement(1825..2493)
                     /locus_tag="VMZ02_02640"
     CDS             complement(1825..2493)
                     /locus_tag="VMZ02_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR70854.1"
                     /translation="MATQAFVDSLIKDIIQPGVDQLVELPYFTELRAGTLSTKRLQGW
                     AMQHYLHNHALLKGFALCMVKNSHEPELFKYFLYQMNEEQYHPDLAKKFGLEIGLQEE
                     DFQKATPVFECLAHTSKTIHGMLLGTAPENRASALVNETMVCRYSEEHIAALKKHYGL
                     NDKQIQFFTVHAVADQEHTAMASEIIAKHANTEHQEQLVREAAAHMVRFKIAKFHGIY
                     NAYA"
     gene            complement(2495..3487)
                     /locus_tag="VMZ02_02645"
     CDS             complement(2495..3487)
                     /locus_tag="VMZ02_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70855.1"
                     /translation="MKTLRKHHRHLPVRALLVWALGILVVQFPGANAQELKKVRMGYP
                     AFSLTFLTFFVAKDAGIFKKHGLDVDLVQLAGAVQTSALVSGEIDYLTGITGPLVAAA
                     RGLPFKGIMVTHDRTLFWLIGNPDMRRAEDLVGKTVAVDRLATLQDIVARDLIKRKGV
                     NPDLVTYIQTGSVSNSVQSLSQGSVAAAVLSLPHNVTMVQKGYREIASAPEFNMRSAS
                     GGMAIHEGKLKKDPAGVKTVIRAVFEAMDFNRREKIWMVNYIQNKWRVSAKVAEESYK
                     SWLNGFTVDGKIPLKDLQEIYDQAYAAKLIPTAVPAAQVMDYTLTDEVFKEKRN"
     gene            complement(3500..3622)
                     /locus_tag="VMZ02_02650"
     CDS             complement(3500..3622)
                     /locus_tag="VMZ02_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70856.1"
                     /translation="MRIQLEQAAANRASEITVGDSVDESIVRGIKKAGFIDRLY"
     gene            complement(3658..4611)
                     /locus_tag="VMZ02_02655"
     CDS             complement(3658..4611)
                     /locus_tag="VMZ02_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70857.1"
                     /translation="MKSMVLSIILAALMTVDGDAAPVRLSYSVVGPTVASVWMAHETG
                     MFKKYGLDVQLVYIASSGTNIQALLGGSLELSAPGMSGVVLAAARGAPVLTIGAMTNK
                     PPMTLYVQPEITRTDQLKGQSLGITRYNSTAHTVTTLILRKLNMEKAVTQRPLGGAPE
                     VQAAFEQKQLAGMVTAVKPRAPARALLNAADLDIPFAMNVMAVTQEYWRKNPDTVERA
                     MRAYIEAVALMHHDKDTTLKVLQKYFKRSDPGFLEEMYSIVVRYIEKVPRVDPRNVAT
                     TLEFEPVKGVDAESLTAKVIDNSIVDKLVKEGFIDKVFAKR"
     gene            4900..5874
                     /locus_tag="VMZ02_02660"
     CDS             4900..5874
                     /locus_tag="VMZ02_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HUR70858.1"
                     /translation="MAWRKIALIACAICLGSSVGHAQKYPDKVITIVVPFTAGGPTDT
                     VARLVAQPMSTILKGQIIIENVGGAGGTIGANRVAKAAPDGYNLLLHHIGQATAPSLY
                     RKLPYDPVNDFEPIGLINEVPMTIIARRDFPPKDLKELIAYLKTNKEKVTYANAGIGA
                     ASHLCGMLLMSQIEVDLTTVPYKGTADVMKDLLGGQIDFTCDQTTNTTSQIKAEKVKV
                     YGVTTKTRLATFPKIPTVDEAGLKGFEVSIWHALYAPKGTPKPVIEALTKALQAALKD
                     KIVKQRFADLGTEPVAEKRATPEALRVQLKSEIDRWAPIIKKAGVYAD"
     gene            5996..>6215
                     /locus_tag="VMZ02_02665"
     CDS             5996..>6215
                     /locus_tag="VMZ02_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70859.1"
                     /translation="MIRSTRDLWSGLIYICFGTAAIILGREYGMGTALKMGAAYFPTL
                     LGWLLVLIGAIALVRAFVAQTEPIGSFSI"
BASE COUNT         1323 a         1598 c         1781 g         1513 t
ORIGIN      
        1 gcacgcgatg ctgagttgca gcttgcgcaa aaaaagaggc cggattcttg tgaatccggc
       61 ctcttgccac tgaacgtgac gggggctcag tggcgccgcg ggtcccgtgg gagcttgctg
      121 aaggagacag cgcgggacgc ggcaagtcaa ttcagattgg agctcctagt cgcttgtgtc
      181 cacttatcgc gttgcagacg gcgttgtaga tacgtgtctt tgggccactt ccaattgcgc
      241 ccgggaagct cttcgccgaa ataggcagcg ccgttcactt tgctttgtag aaatactaac
      301 tgccagtaac ttggtttatt gagatctgtt tccatagctt tcatctttcc gttctccttt
      361 ttctccttat tttttagacg ctgtaacctg tccctctgca tcttttttcg tctcgagtcc
      421 gtctcttgta atgagcccga tagtgcagga gccatgccgg aagttgaatt taccgtggac
      481 ggataggctg tccttaatat tcttaaatta agactcgggc aacgaggcgg cgacgaccgg
      541 cgaaactcgg cgaactttta aatcggtcag aagtgccgtg cgcatgcccg ccgaaaagcg
      601 attggtaact atgtcggcga gtcaaaatat cgttgcgcca tcaataggtt aggctcgaag
      661 tctcgtgtca gtgtcgtaaa atctgcccgc ccatctggcg ctggatagat gagttcaatg
      721 gcatgcagta acgtggcgat tcagactttg aatgtatcga gggttgcgcg ggggaatagt
      781 tcgtcgacgc ccaagttccg gctggcaacg ccttgttcaa tactatagcg gagaaaagtt
      841 tcgagagtct tgcgattggc ctcgacaccg taaggccaga agtcttgacc cattagctgg
      901 cgtacttctt cggcatgatt ggcggcccac ggtatcactg agtgaatcgc gtcattgcgg
      961 aaaattgtgt cgacgcaatg ctgcttggcg tcgagcagcg ctttgtaaag actctgggca
     1021 atccacggat ggcgctggaa gagttgctcg cgcaacacca tgacatgcat gatcggaaaa
     1081 attccggtgc ggcgaaaatg atcggtctca acggcgcgcg gatcggcgaa cagccggcgc
     1141 acttgcgggg cgcgtttgac gaaaggcgca ggcacctgag ctgtcagcaa cgcgtcgatc
     1201 tcacctgcga ccaacatatc ggagaggttt ttgccggttg gaatattttc tatgtcgatc
     1261 tcgggaggca gtttcagcgc gaccttctcc ttgcgttccg aagactccaa cccgccggtg
     1321 agccaatgca tatcactggg gcggacaccg tagtcgtctt gcaagaagcc gcgcagccaa
     1381 acagtcgcgg tcatctgcca ttcgggaata ccaacgcgtt tacctttcaa gtctgtgggc
     1441 ttgtcgatgc cggcagtgct gttgacgtag gcgcaagagt gacgaaattt gcgataaacg
     1501 aatgccggaa ttccgatgaa cgggtaattg ccgcgcgctt taccggcgac gaagctgccg
     1561 aaggacattt cagcagcatc gaattcctga tgcatgccca tgcgccagtg gacttccggc
     1621 ggtcggttga tggtgatgaa gttaatttcc agtccttcgg cgcgcacggc gccgtctttc
     1681 aggggtcgga caatgtcata gtcgccgcag ccgaggtcaa tggttagtaa aggcatggat
     1741 tagctccgtt tgagaatcgg tgaagtgccg cagtaatggc ggagtccatc aaccacgacc
     1801 ccactctcca acaagtcagt tcgatcacgc gtacgcgtta tagatgccgt gaaatttggc
     1861 gatcttgaag cgcaccatat gggcggctgc ctcgcgcact aactgttctt ggtgttcggt
     1921 gttggcgtgc ttggcgatta tttcactcgc catcgccgtg tgttcctggt cggcgactgc
     1981 gtgcaccgtg aagaattgga tctgcttatc gttgaggccg tagtgttttt ttagagccgc
     2041 tatatgttct tcggaatagc ggcaaaccat ggtttcgttg acgagagcgg aagcgcgatt
     2101 ttccggcgcc gttcctaaca gcatgccgtg aatcgtcttg ctcgtgtgcg ccaggcattc
     2161 gaagacgggt gtcgcttttt gaaagtcctc ttcttgcagg ccgatctcca ggccaaactt
     2221 ttttgccaga tcggggtgat actgctcttc attcatttga tagagaaaat atttgaacag
     2281 ctcgggctcg tggctgttct tgaccatgca caacgcaaag cccttgagca gcgcatgatt
     2341 atgcaagtag tgctgcatgg cccagccctg cagccgtttg gttgataggg tgccggcgcg
     2401 cagttcggtg aaatagggca gttcgaccaa ttggtcgacg cccggctgga taatatcttt
     2461 gatgagtgaa tcgacgaatg cttgagtcgc catgttagtt cctcttttct ttgaatactt
     2521 catcggtgag ggtgtaatcc attacctgtg ctgccggcac cgcggtggga atcaatttcg
     2581 ccgcgtaagc ctgatcgtaa atttcttgca agtctttgag tggaatcttg ccgtcgacgg
     2641 tgaagccgtt gagccaggat ttataagact cttcggcgac cttggctgat accctccact
     2701 tgttctgaat atagttaacc atccagattt tttctctgcg attgaagtcc atggcttcga
     2761 aaaccgcgcg aatgaccgtc ttcacgcccg ccgggtcctt tttgagtttt ccctcatgga
     2821 tggccatgcc gcccgaggcc gaacgcatgt tgaattccgg cgccgaggcg atttcccgat
     2881 agcctttttg caccatcgtg acgttgtgcg gcagcgagag cacggcggcg gcgacgctgc
     2941 cttggctgag tgactggacg ctgttcgaca ccgacccggt ctgaatgtaa gtcaccagat
     3001 cggggttgac gcctttgcgt ttgatcaggt cacgcgcgac gatgtcttgc agcgtcgcta
     3061 gacggtccac cgcgacggtt ttgccgacga gatcttcggc gcggcgcatg tctggattgc
     3121 cgatgagcca aaacagcgtg cggtcgtggg tcaccatgat gcctttgaac ggcaacccgc
     3181 gcgcggcggc gaccaatgga ccggtaatgc cggtcaggta atcgatttcg cctgacacta
     3241 atgcagacgt ctgtactgct ccggcaagtt ggacgagatc gacatcaagg ccgtgctttt
     3301 tgaagatgcc cgcgtctttc gcgacgaaga acgttagaaa agtaaggcta aacgccgggt
     3361 agcccatgcg aactttcttg agttcttggg cgttagcgcc agggaactgg acaacgagga
     3421 tccccagcgc ccatacgagt agcgctcgta ccggcagatg tcggtgatgt tttcgcaaag
     3481 ttttcatagt tgtctcttat taatagaggc gatcgatgaa gccggccttt tttatcccgc
     3541 gcacaatgct ttcgtcaacg ctatcgccaa ccgtgatttc gctcgcccgg tttgccgcgg
     3601 cttgctcgag ctgaatgcgc acattggatc cgcgaattga taatctaaag ccaatcgcta
     3661 gcgtttggcg aacactttgt cgatgaaccc ttctttgacg agtttgtcga caatgctgtt
     3721 gtctatcacc ttggctgtca acgattcggc gtcgacgcct ttgaccggct cgaattccaa
     3781 ggtggtggcg acgttgcgcg gatcgacgcg cggaactttt tcaatgtagc ggacgacgat
     3841 gctgtacatt tcttccaaga agccgggatc gctgcgcttg aaatattttt gcagcacctt
     3901 gagcgtggtg tctttatcat ggtgcatcag tgccaccgct tcgatgtaag cgcgcatggc
     3961 gcgctcgacg gtatcgggat tcttgcgcca atactcttgg gtgaccgcca tgacgttcat
     4021 ggcgaacggg atgtcgagat cggcggcgtt tagcagggcg cgtgccgggg cgcgcggttt
     4081 gactgccgtc accatgccgg ccaattgttt ctgttcgaac gctgcttgga cttccggcgc
     4141 gccgccgagg ggccgctggg ttacggcctt ctccatgttg agcttgcgca gaatcagcgt
     4201 ggtcacggta tgcgcagtcg aattgtaccg agtgattccc agggattggc ccttaagctg
     4261 atcagtgcgg gtgatctccg gctggacgta tagcgtcatt ggcggtttgt tggtcatggc
     4321 gccgatcgtc agcaccggcg caccgcgcgc agcagccagg acaacgccgc tcatgcctgg
     4381 cgcgctgagt tcaagactgc cgccgagcaa ggcttggata ttggtcccgc tcgacgcgat
     4441 gtaaaccagt tggacgtcga gaccgtattt cttgaacatg ccggtttcat gggccatcca
     4501 gacgctggcg actgtcgggc cgacgactga ataggacagg cgcacgggtg cggcatcgcc
     4561 gtcaaccgtc ataagcgcgg ctaaaatgat cgacagcacc attgatttca tggcggcgag
     4621 tgtagccgtg acagcggtga atttcgaggg ggcaataagt ggatcggcct cggtcgtgca
     4681 ctccttgaac gttaagcggc agatgcccac actctctagg aaatgttgag tatgggaacg
     4741 aagtgccgcc tttgtttcac atttgggaaa atgatatggc cttttgccac aactcagact
     4801 caaacatgtg gtatcggtct tgcgctaagg tctcggcata atgaactctc gagtagcgaa
     4861 ctcaactaat cgtctaaaac ctagaaaggg aggaatggta tggcttggag aaaaatcgct
     4921 ttaatcgcgt gcgcgatatg tctgggcagc tcagtgggtc atgcacaaaa atatcctgac
     4981 aaagtcatta cgattgtcgt gccgtttact gccggcggac cgacggatac cgttgctcgt
     5041 ttggtggctc agccgatgtc caccattctt aaggggcaga tcatcatcga aaatgtcggc
     5101 ggcgcgggtg gaaccatcgg cgctaaccgt gtggccaaag ccgcgccgga cggctacaac
     5161 ttattgttgc accatatcgg tcaagccacg gcgccctccc tttatcgcaa actgccctat
     5221 gatccggtca acgacttcga gccgatcggc ttgattaatg aagtgccgat gacgatcatc
     5281 gcgcggcgcg actttccacc caaggatctg aaggagctga tcgcctacct caaaaccaac
     5341 aaagagaaag tcacgtatgc taacgccggt atcggcgccg cctcgcatct ctgcggcatg
     5401 ttgctcatga gccaaatcga ggtcgatctg acgaccgtgc cttacaaggg caccgccgat
     5461 gtgatgaagg atcttttggg cggtcagatt gattttactt gcgaccagac aacaaatacc
     5521 acgagtcaga tcaaagccga aaaagtcaaa gtctatggtg tcaccaccaa gacccgattg
     5581 gccacttttc cgaagattcc gacggtggat gaggccggac tcaagggctt tgaagtgtca
     5641 atatggcacg cgctatatgc gcccaaagga acgccgaaac cggttattga agccttgacc
     5701 aaagcattgc aggcggcgct caaggacaaa atcgtgaagc agcggtttgc cgatctcggc
     5761 acagaaccgg tcgctgagaa gcgtgctacg ccggaagcgc tacgagtcca attaaagtcg
     5821 gaaatcgaca gatgggcgcc gattatcaaa aaggcgggtg tctacgcaga ttgagcttaa
     5881 gtttagcgag cgaccaaaag gccgggcttt gtcccggcct ttttcgcttt tgttttggcg
     5941 cgacctggga tacaaggcgc gagaacaatc ataagcattc aacgattggg gtctaatgat
     6001 ccgtagcacc agagacttgt ggagtggtct gatttacatc tgctttggca ctgccgcgat
     6061 tattctcggt cgtgagtatg ggatgggcac ggcgctgaaa atgggcgcgg cttatttccc
     6121 gacgttgcta ggttggttgc ttgttctgat cggtgcaatc gcgctagtcc gtgctttcgt
     6181 ggcacaaacg gagccgattg gatcattttc cataa
//
LOCUS       DATLCH010000090         3228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2519912, whole genome shotgun sequence.
ACCESSION   DATLCH010000090 DATLCH010000000
VERSION     DATLCH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519912"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..986
                     /locus_tag="VMZ02_02670"
     CDS             189..986
                     /locus_tag="VMZ02_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR70860.1"
                     /translation="MFAQIKHAAIYTQNPQNAIAFYEKILGMKRITSAVTENNRGHIS
                     DGLIGLAVLARRPGIPAGLDHFGFEVDDVDRARQRIKKHYPEAIITKGLEKVPFALFR
                     SHDPAGAQFDISERTDPKVREGYKESGWEQTRQFNHVVIRAHKPAAVADFYQEVFELF
                     AVKEPPLGETICLSDGRVRLLIQPTSDNSYMSMRQGLDHIGYRVENVEQMRKDLEQLA
                     VSVPASAPRDIAGGIFGHLTKRDMDGCKIGQYATSDPDGVLLDFSEA"
     gene            1133..2053
                     /locus_tag="VMZ02_02675"
     CDS             1133..2053
                     /locus_tag="VMZ02_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70861.1"
                     /translation="MIIFIHAAPAPAQKLTQIHIGVPTHTVTWFPLYVAWKKGIFQEY
                     GIELLPVTMSVRASLGALASRHVSYITPLGSSLTAIANGLPAKVIMIFAVRSHHVLVA
                     KRDIAGPSALRGRSVAISQPGGTVHRQFLKILEHYRVDPKSVNMINLGEMPARALALK
                     ANNIDAAMLSVPYDIFMEKDGFRPIAYMKDIAEFPLLGLIAHDDRLREKSEEVRKILA
                     AALKGIAYTKSHRDDVLPLLKQFAGLPDIEMTRKTFDVIKDIWPDNGIPSNTGLATAI
                     SMADVPANFPTDKLVDWSPVSETSASLKAR"
     gene            2105..>3228
                     /locus_tag="VMZ02_02680"
     CDS             2105..>3228
                     /locus_tag="VMZ02_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70862.1"
                     /translation="MSETILDADSHVSEPLNLWQERLPEKYRAMAPHMETEYRGQPGA
                     WWHIEPDRQPHNVILGFGANKSMDELQKLLESFSYAGAHRGGWDPAQRLKDMDQDGVA
                     GDVLYTTLGFRMFWIKDAGFQRACFQVYNEWLAEFCAHSPKRLKGLALISLYDPKQAA
                     IDLADCAKKGLAGAIIWASPPDDLPFHSDSYDPFWAAAQELKMPLSLHEFAGFKWVDW
                     DSNSKKRTVAQAINSHEVEQTFATLIVSGVLERFPNLKVVSAELNCGWVPFFLHRMDE
                     RFDHRGIRFRGTPFNTKLTLKPSDYFRRQLYATFIGDTFGLAHRENIGVENIMWSSDF
                     PHSATFWPKSREKIAADFHTVSATDQEKILCQNTAKLYGF"
BASE COUNT          783 a          917 c          846 g          682 t
ORIGIN      
        1 tgtcatttcg acgcgaagcg gagaaatctt gtctttctat gtgtacaaaa tgcaagatct
       61 ctcacgccgt tcgagatgac agatcagaga gacgctgagt tttcacacag tctggcgctc
      121 gggattacag aaataaggaa gaacagcatt caagatagag tccgtaaaaa tctgccgagg
      181 agcttttcat gtttgcccaa attaaacacg ccgcgattta cactcagaat ccgcagaacg
      241 ccattgcctt ctacgaaaaa atcctcggca tgaaacgcat caccagcgca gtgacggaaa
      301 acaatcgcgg tcacatcagc gatgggttaa ttggactcgc ggtcctggcg cgccggccgg
      361 gtattcccgc gggactggat catttcggct tcgaagtcga tgatgtcgac cgcgcccgtc
      421 agcgcataaa aaagcattat cctgaagcga tcattaccaa agggctggag aaagttcctt
      481 tcgcgttgtt tcgcagccat gatccagccg gtgcccagtt tgacatctcc gagcgcaccg
      541 atcccaaggt cagagaaggc tacaaagaat ccggctggga gcagacccgg caattcaacc
      601 atgttgtcat ccgtgcccat aagccggcgg ccgttgcgga tttctaccaa gaggtgttcg
      661 agctctttgc cgtaaaagaa ccgccgttgg gcgaaaccat ttgtttgagt gacggcaggg
      721 tgcgtttgct gattcaaccc accagcgaca actcgtacat gagcatgcgc caaggtctcg
      781 atcacatcgg ctatagagtc gaaaacgtcg agcaaatgag aaaagacctc gaacaactag
      841 ccgtcagcgt accggcatcc gcgccgagag atatagccgg cggaattttc ggccacctca
      901 ccaagcgcga catggacggc tgcaagatcg gccagtacgc gacttccgat ccggacggcg
      961 tgttgttgga cttttccgag gcctgatgag aaccgacatg agcggcgctc tagcatcgca
     1021 actaaattat catcaccgcg aatctccgag tgccgccctg tgcaatggga gattaggagg
     1081 gcttttacgg ctacggcaaa cagctcgaag caaaagtctc gtcgcaattt gcttgattat
     1141 tttcattcac gccgcgccgg caccggcgca aaagctcacg cagattcaca tcggcgtgcc
     1201 gacccatacc gttacctggt ttccactcta tgtcgcttgg aagaaaggaa tctttcagga
     1261 gtatggcatt gagctattgc cggttaccat gtcggtacgc gcctcattgg gcgcgttagc
     1321 atcgcgccat gtctcctaca tcacgccact cggctcatcg ctgacggcga tcgccaatgg
     1381 actacccgcc aaggtgatca tgattttcgc cgtgcgctca caccacgtct tggtcgctaa
     1441 gcgggatatc gccggtccca gcgcgctgcg cggtcgcagc gtcgccatca gccaaccggg
     1501 cggcaccgtg catcggcagt ttttgaagat tctcgagcat tacagagtcg atcccaagag
     1561 cgtcaatatg atcaacctgg gcgagatgcc ggcccgtgct ctcgccctta aggccaataa
     1621 catcgatgcc gcgatgttga gtgtgccata cgacatattt atggagaaag atggttttcg
     1681 tccgatcgct tacatgaaag acattgccga gtttccgctg ctcgggctca tcgcccacga
     1741 cgatcgcctg cgcgaaaaat ctgaagaagt aagaaaaatt ctcgccgccg cgctcaaagg
     1801 catcgcctac accaagagcc atcgcgatga cgtattgccg ctgctcaaac agttcgccgg
     1861 cctgccggac attgagatga cgagaaaaac tttcgacgtc attaaggaca tttggcccga
     1921 caacggcata ccatcgaata cgggtctcgc caccgcaata agcatggccg atgtgccggc
     1981 aaattttccc accgacaagc tcgtcgattg gtcgccggtc agtgaaacct cggcgtcttt
     2041 gaaagctcgt tagaatggaa tatctatagt gaagctaagc aaaacttaat aaaaggaaaa
     2101 tcctatgtcg gaaacaatct tggatgcgga ctcccatgtc agtgagccat tgaacctgtg
     2161 gcaggaacgg ctgccggaaa agtatcgggc catggcgccg catatggaga cggaataccg
     2221 gggtcagccc ggcgcgtggt ggcatatcga accagaccgc cagccgcaca atgtcatcct
     2281 gggcttcggc gccaacaaat cgatggacga gctgcaaaaa ttactggaaa gcttctcata
     2341 cgcaggcgct catcgaggcg gctgggaccc ggcgcagcgg ctgaaagaca tggaccagga
     2401 cggcgttgcc ggagacgtgc tctacacgac gctcggcttt cgcatgtttt ggatcaaaga
     2461 tgccggcttt cagcgtgcct gctttcaggt ctacaacgaa tggctggccg aattctgcgc
     2521 ccactcaccc aagcgcttga aaggtctggc gctgatctcg ctctacgatc ccaagcaggc
     2581 ggcaatcgat ttggccgact gcgcaaaaaa gggtttggcc ggggcaatca tttgggcgtc
     2641 accgccagac gacctgccgt ttcattcgga cagctacgat cctttttggg ccgcggcgca
     2701 ggagcttaaa atgccgctca gcctacacga gttcgccggc tttaaatggg tcgattggga
     2761 ttccaattca aaaaaacgca ccgtcgcgca agcgattaat tcccacgagg tcgagcaaac
     2821 ctttgcgacg ttgatcgtct ccggcgtgct cgaacgcttt cccaatctca aagtagtctc
     2881 ggcggaactc aactgcggct gggtaccgtt ttttctgcac cgcatggacg agcgcttcga
     2941 tcaccgcggc atccgttttc gcggcacacc gttcaatacc aagctgacgc tcaaaccgag
     3001 cgattatttt cgccgccagc tttatgcgac cttcatcggc gacaccttcg gtctcgccca
     3061 ccgggaaaac atcggcgtcg aaaacatcat gtggtcttcc gactttcccc acagcgcgac
     3121 cttctggccc aaatcgcggg aaaaaatcgc cgccgatttt cacactgtca gcgcgacaga
     3181 tcaagaaaaa attctttgtc aaaacacggc caagctgtac ggcttcga
//
LOCUS       DATLCH010000091         6540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9227298, whole genome shotgun sequence.
ACCESSION   DATLCH010000091 DATLCH010000000
VERSION     DATLCH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9227298"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VMZ02_02685"
     CDS             complement(<1..396)
                     /locus_tag="VMZ02_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUR70863.1"
                     /translation="MASDHHHESHHHANHGHGHIDYRFCPKCGGGLDKRILKANEPSR
                     LICQACSFIFYQDPKVVAGTVFTLDGGIVLLKRGVEPALGKWVFPGGYVDRGESVAEA
                     AVRETKEECLLDVKLGSLLNVYSYPRSPTV"
     gene            456..1133
                     /locus_tag="VMZ02_02690"
     CDS             456..1133
                     /locus_tag="VMZ02_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HUR70864.1"
                     /translation="MLRAVIFDFNGIIVDDEPIHFELFRKVFSEEGIDLTKQAYYERY
                     LGFDDRGAFSFGFGEHGRPLSHQKLTELIDRKALYYQQAIRNHVTIFPGAKELIVNLA
                     DDLPLAVASGALRNEIETILTTAGLIRHFKAIVSAEDVERGKPEPDIFIKALAILNAG
                     MDNAIQASDCIVIEDSKEGIKGARRAGMKCLAVTNSHPAELLGEAHAIVKSLEEVGLA
                     RLAQLCA"
     gene            complement(1282..2346)
                     /locus_tag="VMZ02_02695"
     CDS             complement(1282..2346)
                     /locus_tag="VMZ02_02695"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR70865.1"
                     /translation="MPMIDADTHVDESEDTWKKLEGTPYAKYIPVTTTMPPDEAKRAG
                     YNPTNSRCWVVEGRLQNRAIRDEINHPPRVLRELQDVPGRVAHMDKMGVAVQVIFPTF
                     FIRYNTSNAEAEWALTTTYNRWLADKCAPTNGRLRWAAVLPLLSPEKAVAELRWAKEH
                     GACGIFKRGFDLDRYVTDPHFFPVYEEASSLDLPLCIHTGHPLPGHEWDRGFPVMYSC
                     HAVVSSGIAKQFPKLRFGFIESGASWIPYTVSQLGAVKRQSLRGQGTRLPNLYELEPD
                     MFRANRLFVTIDPIDDIQSLLKFGTEDNLMIGTDYSHTDISANLTALEEVRGWVEEGR
                     INNTQAQKILETNSKEFYRL"
     gene            complement(2512..3219)
                     /locus_tag="VMZ02_02700"
     CDS             complement(2512..3219)
                     /locus_tag="VMZ02_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR70866.1"
                     /translation="MNSVAGKTALVTGAGRGIGRAIAVALAKAGCTVVVTARSLAELL
                     AVEQEIVAANGNARVVQADLTRDDELHSLVQACGAVDFLINNAGWGKRAPVVRSRVDD
                     WDQTFRLNLRAPMILAKELLPPMIRKGDGAVINIGSVSGKSGEANGAAYSASKFGLIG
                     FTQSLFEEVRENGIKVAVILPGFVDTPMIPPVKQLDRSRMIQADDVAEAVLYVLTSPA
                     TACPIEITLRPQRTPYR"
     gene            complement(3401..3820)
                     /locus_tag="VMZ02_02705"
     CDS             complement(3401..3820)
                     /locus_tag="VMZ02_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUR70867.1"
                     /translation="MTDEEAVGEANAGFYRAFETLKLDAMEAVWAKDTDIQCGHPGWR
                     ILRGWGPVMESWKRIFENTPSMQFTLTDVNINVSGEVAWVTLYENLNSSIQGQIFSAA
                     ILTTNVFLKTADGWRMILHHGSSVTQSTDQDESSTVH"
     gene            complement(3973..4299)
                     /locus_tag="VMZ02_02710"
     CDS             complement(3973..4299)
                     /locus_tag="VMZ02_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70868.1"
                     /translation="MSNFKKLSDAINFSPEKMKKNGIFETEHFFCDTYCFEPGQEQKP
                     HTHAAEDKIYYVLEGRGAFTVGTEERELAAGEIVLAPAGQSHGVANRSQARLVTLVFV
                     TPKPHH"
     gene            4385..5584
                     /gene="thiI"
                     /locus_tag="VMZ02_02715"
     CDS             4385..5584
                     /gene="thiI"
                     /locus_tag="VMZ02_02715"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HUR70869.1"
                     /translation="MDSVLIRYHEIALKKGNRAYFTELLKRNILAAVKDLGAKEIRSL
                     PARLLLTFRNEIDPDTLIKRMSSVFGIANFSLVQRTECDIEQLRRQILTGLDGAPFSS
                     FRIDTQRGDKTFPLNSPEINRQLGAAVQEKSGARVDLTNPEFTVHVEILPKDAFFGYN
                     KLPGAGGLPVGASGRLMALISGGFDSPVAAYRMMQRGCRVNFVHFHSAPYQDKTSQDK
                     VQQLVKLLTRHQFQSRLYMVPFGEIQRQIVAAVARPLRVVLYRRMMLRIAEQIARKEK
                     ARALITGESLGQVASQTLENMTVIQQAVTMPILRPLVGMDKQEIIDQARRIGSFETSS
                     IPDQDCCQLFVPKHPATKGRLDDVEADEAKLQVTELVRYGLESASNEEFVFPSAVPDV
                     ENRKAMC"
     gene            complement(5724..>6540)
                     /locus_tag="VMZ02_02720"
     CDS             complement(5724..>6540)
                     /locus_tag="VMZ02_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR70870.1"
                     /translation="ILEPEKFRSFELAGWQAIPAGYHDTFGPLTSQAIEPLLDAVRLK
                     KAMSLLDIASGPGYVAAAAAKRGAAVVGVDFSAAMVEHAQRLHAGVEFREGDAEALPF
                     GNGLFDAAAMNFGILHLGQPEKALIEARRILRTNGRFAFSVWAKPEETIGFGIVLRAV
                     ELHGEPRVELPEGPPFFRYSDPEECQRSLLVAGFETPSVIKVQQVWRLPAGDGLFNAM
                     KDSTVRTAGLLRAQKPTVLTKIRDEMRGSLAQYTKRDVVELPMPAWVASGIKA"
BASE COUNT         1482 a         1817 c         1772 g         1469 t
ORIGIN      
        1 gacggttggc gatcgcggat acgaataaac gttgagcagc gaaccaagtt ttacgtctag
       61 caagcattct tctttggtct cgcgaaccgc cgcttcggca acgctctccc cacgatcgac
      121 ataaccgccg gggaataccc atttgccgag cgccggctct accccgcgct tgagcagaac
      181 gatgccgcca tccaatgtga acacagtgcc ggcaaccacc ttagggtctt gataaaaaat
      241 gaacgagcaa gcttggcaga tcaatcggct gggctcgtta gctttaagga ttcttttatc
      301 tagaccgccg ccgcacttgg gacaaaagcg ataatcgatg tgcccatgcc catgattggc
      361 gtggtgatga gactcatgat gatgatctga tgccatggtt atttttctag cgcagatcgc
      421 gttgaaacaa aagatgaatc ctgtaacttg ggaaaatgct gcgcgcggta atttttgact
      481 tcaacggcat catagtcgac gatgagccaa tccattttga actgttccga aaggtttttt
      541 ccgaggaagg gatagatctc accaagcaag cctactatga acgctatttg ggtttcgacg
      601 atcggggtgc cttcagcttt ggctttggcg aacatggtcg gccgttgagc catcaaaaac
      661 tcacagaatt aattgaccgt aaagcgcttt actatcagca ggcgatccgc aatcacgtca
      721 cgatctttcc cggcgcgaaa gaactcatcg tgaatttggc cgacgatttg ccgctcgcgg
      781 tagcttccgg cgcgctgcgc aacgagatcg aaaccatttt aactaccgct ggcttaattc
      841 gtcatttcaa agccatcgtc agtgccgaag atgtcgaacg gggcaaacca gaacccgaca
      901 tctttatcaa agctttagcc atcttgaacg cgggaatgga caacgccatt caagctagcg
      961 attgcatcgt catcgaagac tcgaaagaag gcataaaggg cgcccggcgc gccggcatga
     1021 aatgtctggc ggtcaccaac tcgcatccgg ccgagttgct gggcgaagcc cacgcgatcg
     1081 ttaagagcct cgaggaggtc ggactggcgc gcctagcgca gctttgcgcg taaccgaact
     1141 gtaagtctct cctgccagca catcaaagat aatgatggat gccggtttcc accggatgac
     1201 gagttttatc ttggcggcga cgtgtgttgg acgatgttaa acgcttcagc gcatttacaa
     1261 taacgttctt tgcggctgac gctatagccg gtaaaactcc ttggagttgg tctcgagaat
     1321 cttttgcgcc tgggtgttgt tgatgcggcc ttcctcgacc cagccacgca cttcctccaa
     1381 cgctgtcaga ttggcggata tatcggtatg gctgtaatcc gtgccaatca tcagattgtc
     1441 ttcagtgccg aactttaaaa gcgactggat gtcatcgatg gggtcgatag ttacgaacaa
     1501 gcggttggcg cggaacatat ccggttctag ttcgtaaaga ttgggcaagc gcgtcccttg
     1561 accgcgcagc gactggcgct tcaccgctcc caattgcgaa accgtataag gaatccacga
     1621 agctcccgat tcgatgaagc caaatcttag tttgggaaac tgcttggcga tgccggagct
     1681 gacgaccgcg tggcacgagt acatcaccgg aaagccgcga tcccattcat ggccgggcag
     1741 cgggtgccct gtatggatgc ataaggggag gtcgagggag ctagcttctt cgtagacggg
     1801 aaagaaatgt ggatcggtta cataccggtc aagatcaaat ccgcgcttaa agatgccgca
     1861 ggcgccatgt tctttcgccc aacgcagctc cgccactgct ttctccgggc tcagcaacgg
     1921 cagcaccgcc gcccaacgca gccggccgtt tgtcggcgcg cacttatcgg ccaaccaacg
     1981 attataggtt gtggttagcg cccactcggc ctcagcgttg ctggtgttgt agcggatgaa
     2041 aaatgtcgga aagatgacct gaacagcgac acccattttg tccatgtgcg caacccggcc
     2101 cggcacgtcc tgaagttctc gaaggacacg cggcggatga ttgatttcgt cgcgaatggc
     2161 gcgattttgc agtcgccctt cgacgaccca acagcggctg ttggttgggt tataaccggc
     2221 gcgcttggcc tcgtctggag gcatcgttgt ggtcacgggg atgtacttgg cgtatggcgt
     2281 accttccaac ttcttccaag tgtcttcgct ttcatcgacg tgcgtatcgg catcgatcat
     2341 gggcatcgga gaacctccac aaaaaccatc agatttttct tacaataggg ttacagttta
     2401 tggtttttcc tgtcaaatcg tggactccat agtaagcgcc ccgaagacac aggggagtca
     2461 tcgcaagtca gttatcttag gcaaaaagat cccgcttgga aaattcttaa atcaccgata
     2521 gggagttcgc tgcggccgga gggtaatttc gatcgggcag gctgttgccg gagaggtgag
     2581 cacataaagc acggcttcgg cgacgtcgtc ggcctgaatc atcctgctgc ggtccagttg
     2641 cttcaccggc ggaatcatcg gcgtgtcgac gaagcccggc aagatcacag cgactttgat
     2701 gccattctcg cgaacctctt caaagagcga ttgtgtgaag ccgatcagac cgaacttcga
     2761 agcggaataa gcggcgccat tggcttcgcc gctcttgccc gagaccgaac cgatgttgat
     2821 aacagcgccg tcgcctttcc tgatcatcgg cgggagtaat tcttttgcta ggatcatcgg
     2881 cgcccgcaaa ttgaggcgaa acgtctgatc ccagtcgtcg acgcgcgaac gcacgactgg
     2941 cgcgcgtttg ccccaaccgg cattgttgat caaaaagtcc accgcgccgc acgcctgcac
     3001 caatgagtgc aactcgtcat cacgagtcag gtcagcttga accacgcggg cattgccgtt
     3061 tgcagcaact atttcttgct ccacagcgag taattcagcc aagctacgcg cagtaacgac
     3121 aaccgtacac ccggctttgg ccagtgcgac cgcgatggcg cggccgatac ctcgacctgc
     3181 acctgttaca agggcggttt tcccggcaac gctgttcatt cgtgtcccgt catccgagag
     3241 aaaagccggt ctcgtatcga gccggtgttt gaatcgaaaa ttttttcaga gttgaagctg
     3301 gtttggcccg cctaacgatt ttctacagga cggttttcaa cacggcccgt cggtcgacga
     3361 cacaaattcg cccgcgcatc tgcagttttg acgatattgg ctagtgaacc gtagaacttt
     3421 catcttgatc ggtcgactgc gtaaccgatg agccatgatg caatatcatg cgccagccat
     3481 cggcggtttt tagaaacaca ttcgtggtca ggatagccgc ggaaaaaatt tgtccttgga
     3541 tcgagctgtt caagttttca taaagggtca cccaggcaac ttcgccgctc acgtttatat
     3601 ttacatcagt caaggtgaac tgcatggacg gcgtgttttc aaagatgcgc ttccaactct
     3661 ccatcaccgg accccaaccg cgcagaatgc gccagccggg atgaccgcat tgaatatcgg
     3721 tgtctttagc ccaaacagct tccatcgcat cgagtttaag agtctcgaag gcgcgataga
     3781 aacccgcgtt ggcttcgcca actgcttctt cgtcagtcat gatcagcgac tctttcgtag
     3841 caaattcgca cttcgctgtt gcgcgccagc ggcacagagc ttcgcagcat tttggactcg
     3901 caggtgctgc gaactcgagg gtcagcgcgc ccctgcggtg caattaccag cgaaagaaaa
     3961 ggcataatgc tgttaatggt gcggtttggg cgtcacgaac accagcgtaa cgaggcgtgc
     4021 ttggcttcgg ttggcgacgc catgactctg cccggccggc gcgaggacga tctcccctgc
     4081 tgccaactcg cgctcttcag tgcccacagt aaacgcgccg cgcccttcga gaacgtaata
     4141 gatcttgtcc tcggcggcgt gggtgtgtgg tttctgctcc tgtcctggtt cgaagcaata
     4201 ggtgtcgcag aaaaagtgtt cggtttcgaa aatgccgttc tttttcatct tttccggcga
     4261 aaaattaatc gcatccgaca attttttgaa gttgctcatg gctcactcct ctcgagtgtt
     4321 gtcggcgata ctaccataga ggtgacggca tgcaaaagca ggatgaatct gatagttctc
     4381 gtgcatggac tccgtgctga tccgctatca tgaaatcgcg cttaagaaag ggaatcgagc
     4441 gtatttcacc gaattgctca aacgcaacat cctcgcagcg gtcaaagacc ttggcgcaaa
     4501 agaaattcgc agcttgccgg cacgcctgct gttgaccttt cgcaacgaaa tcgatcccga
     4561 cacgctgatt aaacgcatgt ccagtgtgtt tggcatcgcc aatttttctt tagtgcagcg
     4621 caccgagtgc gacatagagc aactgcgcag gcaaattttg actggactcg acggcgcacc
     4681 tttctcgagc tttcgcatcg acacccagcg cggcgacaag acgttcccgc tcaactcgcc
     4741 cgagatcaat cgccaacttg gcgccgcggt acaggagaag tccggcgccc gcgtcgatct
     4801 caccaatccg gaatttacag tacacgtcga gatcctgccc aaggacgcat tcttcggcta
     4861 caacaaactg ccaggcgccg ggggtctgcc ggttggtgcc agcgggcggt taatggccct
     4921 catttctggc ggcttcgatt cgccggtggc ggcttaccgt atgatgcagc gcggctgccg
     4981 cgtcaacttc gtgcattttc atagcgcgcc gtatcaggac aagacctccc aggacaaagt
     5041 gcagcaactc gtcaagcttc tgacgcgtca tcagtttcaa tcgcgcctct acatggttcc
     5101 gttcggcgag attcagcgtc agatcgtcgc cgccgtggca cggccgttac gtgtagtctt
     5161 gtatcgccgc atgatgctgc gtatcgccga acagatagca cgcaaagaaa aagctcgcgc
     5221 gttgatcacg ggcgagagtc tcggccaggt ggcatcacag acattggaaa acatgacggt
     5281 gattcaacag gctgtaacga tgccgatctt gcgtccacta gtgggcatgg acaagcagga
     5341 gatcatcgat caagcgcgcc gcatcggcag cttcgaaact tcttcgatcc cggatcagga
     5401 ctgctgtcag ctcttcgtgc cgaaacatcc cgcgaccaag ggcagactcg acgacgtcga
     5461 agccgatgag gcgaaactgc aggtgaccga attggtccgc tatggactcg agagtgcgag
     5521 caatgaggaa ttcgtgtttc cgagcgctgt cccagacgtc gaaaacagaa aagctatgtg
     5581 ttaacgccgc aaaacgatcg gcgaacgttg gtatcgacgc ttacagacaa attaaaaccg
     5641 aagatggatg ccggttttca ccgacatgac gaattcagag atcccgccgt gcaaaaactt
     5701 ttgaacgatt aaaacaaatc cagttaggcc tttattcctg atgccaccca agcgggcatc
     5761 ggtagctcga ccacgtcgcg tttggtgtac tgcgcgagcg atccgcgcat ttcatcgcgg
     5821 attttcgtta gtaccgttgg tttttgcgcg cgcaacaatc cagccgtgcg cacggtgctg
     5881 tccttcatgg cgttgaataa gccgtcgccc gcgggcaagc gccagacctg ttgaactttg
     5941 atcacgctgg gagtctcaaa gccggcgaca agcaaactcc gttggcactc ctccgggtcg
     6001 ctgtaacgga agaacggcgg accttccggc agttcgacac gtggctcgcc gtgaagttca
     6061 acggcgcgca ggacgatgcc gaagccaatc gtctcttccg gtttggccca caccgagaat
     6121 gcgaagcggc cattggtacg caggatgcgt cgagcttcga tcaaggcttt ctctggctgg
     6181 ccaagatgaa gtatgccgaa attcatcgct gcagcgtcga aaagaccatt gccgaagggc
     6241 agcgcttcgg cgtcgccctc gcgaaactcc acaccagcat gcaagcgctg cgcgtgctcg
     6301 accatcgctg ccgagaaatc gaccccgact acggcggccc cgcgtttggc agctgctgcc
     6361 gccacgtagc ctgggccgct agcgatgtcg agtaagctca tggctttttt caaacgcacc
     6421 gcatcgagca agggttcgat tgcctgcgag gttaggggac cgaacgtatc gtgatagcca
     6481 gcgggtattg cctgccagcc cgccagctcg aagctgcgaa atttttccgg ttccaggatc
//
LOCUS       DATLCH010000092         6064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1783014, whole genome shotgun sequence.
ACCESSION   DATLCH010000092 DATLCH010000000
VERSION     DATLCH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783014"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1096
                     /locus_tag="VMZ02_02725"
     CDS             89..1096
                     /locus_tag="VMZ02_02725"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HUR70871.1"
                     /translation="MARAIGVVGAGGWGTALAKVLADNGEQVTLWCHGLASFNELTES
                     RQNRTYLPGISLPESVAVTRTLTDAVAAKKLVICAVPSHAVREVFAGAAADIDPGSIL
                     LCGTKGLEESTLQTMAELFAEIFGAWGKERHGFLSGPTFAIEVARGLPAAVTVGAYSE
                     RIAREIQEILSTPSLRVYTSLDVVGVQIGGVVKNVIAIAAGISDGLGLGQNARAALIT
                     RGLAEITRLATRMGADSMTLAGLPGLGDLVLTCAGDLSRNRAVGLQIAAGKTLQEIIG
                     ATRTVAEGVRNARALHDLAQRLAVEMPIVEQMYQVLYHGKKPADAVRDLMQRSLKSEV
                     G"
     gene            1173..1337
                     /gene="rpmH"
                     /locus_tag="VMZ02_02730"
     CDS             1173..1337
                     /gene="rpmH"
                     /locus_tag="VMZ02_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HUR70872.1"
                     /translation="MSKRTYQPSNIRRKRTHGFLARMATPGGRNVIKRRRAKGRKKLT
                     VSVPPKRART"
     gene            1354..1713
                     /gene="rnpA"
                     /locus_tag="VMZ02_02735"
     CDS             1354..1713
                     /gene="rnpA"
                     /locus_tag="VMZ02_02735"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HUR70873.1"
                     /translation="MKGTQAFPKSARLRKRPEFLRLSRSGFKFHSENFVVISKPNDHA
                     ESRVGITVSGKVGNSVIRNRIKRQVREFFRRRRAALPHSVDFLVIARNSAVNLTSTVL
                     DDELAKSFSPPHARRQG"
     gene            1718..1930
                     /gene="yidD"
                     /locus_tag="VMZ02_02740"
     CDS             1718..1930
                     /gene="yidD"
                     /locus_tag="VMZ02_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HUR70874.1"
                     /translation="MSRWLLILPIRVYRLTISPWLPPACRFYPTCSDYAQTAISRHGV
                     LKGLVFSLRRFCRCHPWSAGGYDPVR"
     gene            1949..3568
                     /gene="yidC"
                     /locus_tag="VMZ02_02745"
     CDS             1949..3568
                     /gene="yidC"
                     /locus_tag="VMZ02_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HUR70875.1"
                     /translation="MDKRALIGIALSVLVLVGYQEFISRYYGPAPVSQAPVVEKKATA
                     SQEGTAAPTPSVPASATARVAPPNAPSGQAEKQVIVETDNYIAVFTTQGARLRSFQLK
                     HYRASAEPNSPPFEMIAAAKDMPLPLGVHWQTPAPFDDAAVIYSVQGTDVKLTGAAKA
                     KLVFEGQTADGVTITKSLTFTGSLYPIQFDVAVQSPAGNAPVPEVMLTEKSDHTVANP
                     DAPFEGFIALVDNKIKREPSAEAAKGHEFTGDVAWAGFGYTYFFFSLIPDNGSGHKTS
                     VRQNGAALIASLSGPSSTDHYRLFIGPKELELLKSVGKDLDRAIDHGYFGFVSVPLLY
                     VLHFFHRFTGSYGLDIIILTVLIKIIMWPLTHKSFTSMKAMQKLGPQMEKLKERYAND
                     KEKLNKEIMDLYKRNGVNPLGGCLPMALQFPVFIGLYNALSTPIELRHAPFMWIPDLS
                     RPDWQALPFMFNGWHLGIPILTIIMGATMFIQQWMTPSAGDPNQRKMMMLMPLMFTFM
                     FVSFPAGLTVYWLVNNVLGIAQQYWINRSDK"
     gene            3585..4601
                     /gene="jag"
                     /locus_tag="VMZ02_02750"
     CDS             3585..4601
                     /gene="jag"
                     /locus_tag="VMZ02_02750"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HUR70876.1"
                     /translation="MDFVETEGDNIDAAIQNALKALGVGREKITIDIISEGRKGILGF
                     GAQKAKIRASLRKLTVEQDIAPANPQAIEASVHAPNLRPRAPERRAPETRRAEPPRGD
                     PRARAPEPRPQTADRPRPEPRSDSVPRPATALRQQSETRPDAPPPLTAAEIASMVEKA
                     KSALAEILNLMGMNASVETKAGSDADETILEIKSENSGLIIGRKGQTLEALQYLVSRI
                     AGERPGNEAPHIVIDIENYHERRRQTLQDMALRLGEKAKRQRKTVTVDALSAADRKIV
                     HATLQDDQWLTTRSLGQGAYRRLLIIPEGDRRIRDENLTEREGGRPQRGGRPNPPTPE
                     EDDS"
     gene            4768..5862
                     /gene="ispG"
                     /locus_tag="VMZ02_02755"
                     /gene_synonym="gcpE"
     CDS             4768..5862
                     /gene="ispG"
                     /locus_tag="VMZ02_02755"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HUR70877.1"
                     /translation="MIERRKTRQIQVGKVKIGGDAPITVQSMTKTDTRDVRATVQQIW
                     SLEAAGCEIVRCAVPVREAAEQLGEIKKKIRIPLVADIHFNYKLALIAIEQGVDGLRL
                     NPGNIGARKYVEEVVKAASERKIPIRIGVNAGSLEKDLLQKYNGPTAAGMVESGLRHI
                     HILEDVGYSEIKISLKASDPLMMIEAYRLLAAQVDYAFHLGVTEAGTPGIGTIKSAVG
                     LGTLLAEGIGDTIRVSLAADPVEEVRVGLEILKALKLRKDGMFFVACPSCGRADVDLV
                     SLAKTVDERMLPYSNRNIHVAVMGCEVNGQGEARAADLGVAGGKGIGLIFKRGDVIRK
                     VPEAQIVDALMEEVEKLVAEKDAEAPATVN"
BASE COUNT         1390 a         1647 c         1755 g         1272 t
ORIGIN      
        1 gaggggatac cccggcaaga tttcccgcat actgtaagcg ttctgctctg caaaggctgt
       61 ttgagctcat aactgttgtt aatcgattat ggccagagcg attggagtag ttggcgccgg
      121 cgggtggggc accgcgctgg ccaaagtgct cgccgataac ggcgaacaag tgacgctttg
      181 gtgccatggc ctggccagtt tcaacgaact gaccgaaagc cggcaaaacc gaacctacct
      241 ccccggcatt tcgctgcccg aaagtgtcgc ggtcacgcgg acgttaaccg acgcagtagc
      301 agctaaaaag ttagtgatct gcgccgtccc ttcccacgcg gtgcgcgaag tttttgccgg
      361 cgcagctgcc gatatcgacc ccggctcaat tttgctgtgt ggcaccaaag gactggaaga
      421 atcgacgctg cagacaatgg cggaactctt tgcggaaatc ttcggagcat ggggtaaaga
      481 gcggcacgga tttctttccg gaccaacttt tgccatcgaa gttgcgcgtg gcctgcctgc
      541 cgcagtgacg gttggcgcct attcagaacg tatcgcacgc gaaattcagg aaattttgag
      601 tacccccagt ctgcgggtgt atacctcgct cgatgtcgtt ggcgtgcaaa ttggcggcgt
      661 ggtgaaaaat gtcatcgcca tcgccgccgg catcagcgat ggtttgggac ttggacaaaa
      721 tgccagagca gcattgatta cccgcggctt ggcggagatt acccggttgg cgacgcgcat
      781 gggcgccgat tcgatgactt tggcgggctt gccgggattg ggcgacttgg tacttacttg
      841 tgccggcgac ctgagccgta accgtgcagt gggtttgcag attgctgccg gcaagacttt
      901 gcaggagata atcggggcga ctcgcaccgt cgccgagggg gtgcgcaatg ctcgcgcgtt
      961 gcacgatttg gcgcagcggt tggcggtcga aatgcccatc gtcgaacaga tgtaccaagt
     1021 gctctatcac ggcaaaaagc cggccgacgc ggtgcgcgat ttgatgcagc gatcattgaa
     1081 atccgaggtc ggttaaaatt gacttcgctg gagcggtatg attgatatag tcacttggcc
     1141 gcacgggcgg tctgagaggg aagtattcat atatgagtaa aagaacctat caaccgagca
     1201 atattcgaag aaagcgcacc catggatttc tcgctcgcat ggcgacgccc ggagggcgca
     1261 acgtgatcaa acggcggcgc gccaaggggc gcaagaagct gacggtgtcg gtgccgccca
     1321 agcgagcacg aacatagctg cgaggggaag gtcctgaagg gtacacaagc gtttccgaaa
     1381 tcggcccgcc tgagaaaacg acccgaattc ttgcgattat cgcgatccgg ttttaagttt
     1441 cattccgaaa attttgtcgt tatcagcaag cccaacgatc atgccgagtc ccgcgtgggt
     1501 atcaccgtta gcggcaaggt tggcaattca gtgatccgca atcggatcaa acggcaagtt
     1561 agggaatttt tccggcgtcg ccgcgcggcg ttgccgcaca gcgtagattt cctggtcatt
     1621 gcacggaaca gcgcggtgaa cctgacgtcc actgtgctcg acgacgaatt ggccaagagt
     1681 ttctcgccac cgcacgcgcg ccgccaggga tgatcgcgtg agccgctggc tgctaatatt
     1741 gccgattcgc gtttataggc tgacgatctc gccatggctg ccgcccgctt gtcggtttta
     1801 ccccacctgc tcggattatg ctcaaaccgc aattagtcgc cacggcgtac tcaaaggact
     1861 ggttttttct ctacggcgtt tctgccgctg ccatccttgg agcgccggtg gttacgaccc
     1921 cgtccgatga atgtgatcaa ataaattaat ggataaacgc gcactcatag gcatagctct
     1981 gtcggtcctg gtgttggtcg gttaccagga gtttattagc cgttattatg gtcccgcgcc
     2041 ggttagccaa gcgccggtcg tcgaaaaaaa agcaaccgct tcgcaagagg ggaccgcggc
     2101 gccaaccccc agcgtccctg cctcggcaac cgcccgagta gcgccaccga acgcaccgtc
     2161 tggccaggcg gaaaagcaag ttatcgttga aaccgataac tacatcgccg tattcaccac
     2221 ccagggtgcg cggctcagaa gttttcagct aaagcactat cgcgccagcg ctgagcccaa
     2281 cagtccgccc tttgaaatga tcgcggcggc gaaagatatg ccgttgcccc tcggagtgca
     2341 ctggcaaacg ccggcgccgt tcgacgatgc ggcagtgatt tattcggtcc agggcacaga
     2401 tgttaaactc accggcgctg ccaaggccaa attggttttt gagggccaga ccgctgacgg
     2461 cgtgacgatt accaagtcac tgacgtttac cggcagtctt tatccgattc agttcgatgt
     2521 tgcggtgcaa tcgccggcgg gaaatgcgcc tgttcccgag gtgatgctga cggaaaaaag
     2581 cgatcatacg gtcgctaatc cggacgcgcc atttgagggt tttattgctt tagtcgataa
     2641 caagatcaag cgtgaacctt cggcggaagc ggcgaaggga catgaattta ccggtgacgt
     2701 tgcttgggcc ggtttcggat acacttattt cttcttttct ttgataccgg acaatggtag
     2761 tggccacaaa acttcagtgc ggcaaaatgg tgccgcgctc attgcttcgc tgagcggacc
     2821 aagttcgact gatcactatc gtctttttat cggacctaaa gagctcgaac ttctgaagtc
     2881 ggtcggcaag gacctcgacc gcgcgatcga tcacggctac ttcgggtttg tttccgtgcc
     2941 cctgctctat gttctccatt tcttccatcg cttcaccggc agctatgggc tggatatcat
     3001 catattgacg gtgctcatca aaataatcat gtggccgttg acgcataaga gctttacctc
     3061 gatgaaggcg atgcagaagc tcgggccgca gatggagaag cttaaagagc gctacgccaa
     3121 cgacaaagag aaactcaaca aagaaataat ggatctctac aagcgcaacg gcgtcaatcc
     3181 gctgggcggt tgcttgccaa tggcgctgca gttcccggtt ttcataggtc tctacaatgc
     3241 cctcagcacg cccatcgagt tgcgtcatgc gcctttcatg tggatcccgg atttgtcgcg
     3301 gccggattgg caggcgctgc cgttcatgtt taacggttgg catttaggta ttccgattct
     3361 cacgatcatc atgggtgcca cgatgttcat ccaacaatgg atgacgccct cggcgggcga
     3421 tcccaatcag aggaaaatga tgatgttgat gccgcttatg tttacattca tgttcgtatc
     3481 atttcccgcc ggcttgaccg tttactggct ggtgaataac gttctaggta tcgcccagca
     3541 gtactggatc aaccgcagcg ataagtagcc gaggaagatc agctatggat ttcgtcgaaa
     3601 ccgaaggcga taacatcgat gccgccattc agaacgcgct caaagcgctc ggtgtggggc
     3661 gcgaaaagat tacgatcgat atcatttctg aagggcgcaa agggattctt ggatttggcg
     3721 cgcagaaagc aaagattcgc gcttcgttgc gtaaactgac tgtcgaacag gatatagcgc
     3781 cagccaaccc acaggctata gaagcttcgg tacatgcgcc gaaccttaga ccacgtgcgc
     3841 cggagcggcg tgcccccgag acgcgccggg cggagccgcc aaggggtgac cccagagcta
     3901 gagcgccgga accaagaccg caaacagccg atcgtccacg accagaacca cgttcggata
     3961 gcgtcccgcg tccagctaca gcactgcggc agcagtcgga aacgcggcca gacgcaccgc
     4021 ccccgctgac cgccgcggaa attgcgtcca tggtcgaaaa ggccaagagc gcgctggcgg
     4081 aaattctcaa cctgatgggt atgaacgcgt cggtagaaac aaaagccggg agcgatgccg
     4141 atgagacaat actcgaaatc aaaagcgaaa acagcggctt gatcatcggc cgcaaaggtc
     4201 agacccttga agctttgcaa tacctggtca gccgaatcgc cggtgagcgg ccgggtaatg
     4261 aagcgccgca tatcgtcatc gatattgaga actaccacga acggcgccgc cagaccttgc
     4321 aagatatggc gctccgattg ggcgaaaagg ccaaacgcca gcgcaagacc gtgactgtcg
     4381 acgcgctcag cgccgccgac cgaaagatcg tgcacgcgac tctgcaagac gatcaatggc
     4441 tgacgacgcg cagcctcggc cagggtgcct atcgtcggct gttaatcatt cccgaaggcg
     4501 accgaagaat cagagacgag aaccttacgg agcgtgaagg cggccggccg cagcgcggcg
     4561 gtcggcctaa cccgccgacg cccgaagaag atgacagcta accgttaacc tgcactgagt
     4621 caggagacga ggaaaagaac ctcttgccgc gacaaacggc accgcagcat tatcgtatgc
     4681 tgcgcgcctt cacagtgcgt tttgttgaca tcagttgtcg gaaatatcta agttgaccct
     4741 aaagacccgg ttctctatag ttgagtcatg attgaacgcc gcaaaaccag gcaaatccag
     4801 gttgggaaag tgaaaattgg cggcgacgcg ccgattactg tccagtcgat gaccaagacc
     4861 gatactcggg atgttcgcgc aaccgtgcag cagatttggt ccttggaagc agccggctgc
     4921 gaaatcgtcc gctgcgccgt gccggtacgc gaagcggcgg agcaactcgg tgagatcaag
     4981 aaaaaaatcc gtattccctt ggttgccgac atccatttca attataagct tgcgctgatc
     5041 gccatcgagc agggcgtcga cggcttgcgg cttaatcctg gcaatattgg cgcgcgcaag
     5101 tatgtcgaag aagtagttaa ggccgcctcg gagcgcaaga tcccaatccg catcggcgtc
     5161 aacgccggtt ccttagaaaa agatctattg caaaagtaca acggcccgac tgcggctgga
     5221 atggtcgaga gcggactgcg ccatattcat atccttgaag acgtcggtta ttccgagatc
     5281 aaaatatcgc tcaaggcgtc cgatccgctc atgatgatcg aggcgtatcg tctgctcgcc
     5341 gctcaggtgg actatgcttt tcatctcggc gtcaccgaag ccgggacgcc cggcatcgga
     5401 acgatcaaat ccgctgtggg tttagggacg ctcctggccg agggcatcgg cgataccatt
     5461 cgtgtgtcgc tggccgccga cccggtggag gaagtgcgcg ttgggctgga aatattgaaa
     5521 gcgctcaagc tccgcaagga cggtatgttt ttcgtcgcct gtccctcgtg cggccgcgcc
     5581 gatgtcgatc tcgtatcact ggctaaaacg gtggacgaac ggatgctgcc gtacagcaac
     5641 cgaaatattc acgtcgccgt catgggatgc gaagtcaatg gtcagggcga ggcgcgggcg
     5701 gcggatctcg gtgtcgccgg cggcaagggc atcggcctca tcttcaagcg cggtgacgtg
     5761 attcgaaaag ttcccgaagc gcagatcgtc gacgcgctga tggaagaagt cgaaaagctc
     5821 gtcgcggaaa aagatgccga agcaccggcg acggtcaact gatcttttcg ttttattcgt
     5881 caatcccagc tttagtcgag ttcttatgcg atggagtaaa acgctcatcc cgacactgaa
     5941 agaagatccg gccgatgccg aggtagtcag tcacaagcta ttggtgcggg ccggctatat
     6001 tcgtcagatc agccgcggca tctacgatta ttttccgttg gcgttaaagg tgatccgtaa
     6061 gatc
//
LOCUS       DATLCH010000093         5490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4010330, whole genome shotgun sequence.
ACCESSION   DATLCH010000093 DATLCH010000000
VERSION     DATLCH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4010330"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..747
                     /locus_tag="VMZ02_02760"
     CDS             166..747
                     /locus_tag="VMZ02_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HUR70878.1"
                     /translation="MKLGLSNGADNDDPADATAESAIVFLGCFREASSILHEIEVGAN
                     KTDGGGILGEVLVIDFNPQVLHELKLRQIKCLYGDIAHMDTLHHAHIQSARIVACTIS
                     DAILRGTSNLRLLQQARRLSPHARVIAAADAISSAVELYDQGADFVYIARLHSAHHMA
                     ELITRAVMDGEGFKLYCEQEVQSLKQRMEVLPQ"
     gene            1247..1654
                     /locus_tag="VMZ02_02765"
     CDS             1247..1654
                     /locus_tag="VMZ02_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70879.1"
                     /translation="MSNEILIDADPSPSLLSVTHLVYALHALSLLIGITTAATIIGAF
                     VFGLPSIIAVVINYLKRDEARGTFLESHFRWQIRTFWFGMLWSLLGGFLFMTIIGIPL
                     AIAVFIGAGVWVIYRVVRGWLALRDRKPMYINS"
     gene            1902..2927
                     /locus_tag="VMZ02_02770"
     CDS             1902..2927
                     /locus_tag="VMZ02_02770"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HUR70880.1"
                     /translation="MKTDDLRIVKTHPLLAPAILAEEIPLTEAAAGTVYRSRRAIEAI
                     LDGKDARLLVIVGPCSIHDTKAALEYATNLKAIADALAESLLIVMRVYFEKPRTVVGW
                     KGLINDPDLDESYHINKGLRLARQLLADILELGLPAGTEFLDTTFGQFYADLISWGAI
                     GARTAESQIHRELASGLSMPVGIKNRTDGNVQVAVDAIQAARCRHLFPSLTKEGAPAI
                     LETTGNPYAHLVLRGGSESGPNFDTVSLDAAVRLLRAADLPEIVMVDCSHGNSDKDAG
                     RQHDVAAAIMKNLGTSPVRALMIESHLVAGRQNAPITYGQSITDACLGFDETKDLLHR
                     LADAVPK"
     gene            3097..4077
                     /locus_tag="VMZ02_02775"
     CDS             3097..4077
                     /locus_tag="VMZ02_02775"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70881.1"
                     /translation="MKKNLPSIIAPLMMSVLLLAGKSVYALDKVNVTLPSKSFQFIIF
                     PLAKERGYMREEGIDLNTVMMASTPGLQAVLAGEMDFTGSGSSALVAVSQGNAPLKTV
                     LAITDQVLQWLMVRPQYGAFKELKNKKIAVTGVAAVATFMLKKVAPKYGLDANKDLTF
                     LALPPGQRLAALQSGVVDGGLLSSEERFAALDQGMKEILYLGKEVKNSWGTIATNDQF
                     IKTKPKLMQGFTRALLKALRLVKQNREVAMDAMMKFSELDRDLAARTYDGMIGTFTSN
                     GVVDDETQKNDLDIVREVLKVTKDVPIERAYDFSFAKKADMELIQSGWKP"
     gene            4393..5283
                     /locus_tag="VMZ02_02780"
     CDS             4393..5283
                     /locus_tag="VMZ02_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HUR70882.1"
                     /translation="MPPTVDNKSLKKLRSIQRSADRHWVGNGFPVRTLFAYSNLGAVL
                     SPFLLLDYAGPKEFPPTQERLGVGEHPHRGFETVTIVYDGEVEHRDSSGGGGRIGPGD
                     VQWMTAASGVVHEEFHGRDFARRGGMFEMVQLWVNLPARDKLAPPRYQSITSSQIPAI
                     RMPGGEATMRVIAGEFAGAQGPAQTFTPILVGDLRFSKDQRTDLSVAHGHTTALVVLK
                     GAVRVNGTDEITTAEVGVFERAGRSISIESAKDTTALLLCGEPINEPIVGQGPFVMNT
                     SQEIRQALADYQSGKLGHLA"
BASE COUNT         1243 a         1559 c         1500 g         1188 t
ORIGIN      
        1 ccaaaggttg cgataaaaat tcttgtctgc ttgaggacac gctgtggcat ccgtttgaag
       61 gacttggtaa gcccgaaccg ttgaagcatg aactgaaagg cttctggtca tacatacctg
      121 atcgcttaca accacgagat ccaacgccgg cttgcctcgc ttcttttgaa gctcggtctc
      181 tccaacggcg cggacaacga cgacccggct gacgcgacgg cggaaagtgc cattgtgttt
      241 ctcggctgct tccgcgaggc cagctcgatt cttcacgaga tcgaggtcgg cgcgaataag
      301 acagacggcg gcggaatact cggagaagta ttggtgatcg atttcaatcc tcaagtgctc
      361 cacgaactaa agttgcggca gatcaaatgt ctctatggcg acatcgcgca tatggacacg
      421 ttacatcacg cccacatcca gtcagcgagg atcgttgcgt gcacaatctc ggatgcgatt
      481 ttgcgcggca cgtcgaatct tcggctgctg cagcaggcgc gccggctttc accgcacgcg
      541 cgcgtcatcg cggctgccga cgccatttca tcggcggtcg aactgtatga ccagggcgcc
      601 gacttcgttt acatcgcacg cctgcattca gcgcatcata tggccgagct gatcacgcgc
      661 gcggtaatgg acggcgaggg gttcaagctc tattgtgagc aagaggtgca aagcttgaag
      721 caacgcatgg aagttctgcc gcaatgaaaa cctacgactg gccgtcaacg ggtaagcgaa
      781 attatcgatg aaattcgcgg ctctgtactt tttttcgtcg acccgtcgat gttagattgg
      841 acgaaccggt aggtatccga ctattcattc tcgctgttca gaaactgtca aatcacgagg
      901 taaacaaacg tgtgtagaag cattaagacg cttttcaact tccaaccggc tgtgacagag
      961 gaagaaatac gtgcggcctg tttacaattc gtgagaaagc tcagcggctt caacaagccg
     1021 tcaaaggtca acgaggctgc atttttgtcg gccgtcgacg aaatcgcagc gagctcggct
     1081 aagcttctca gctcactcga gacgaatacg cggccgaaga accgcgcaga cgacgctgcc
     1141 aatgcgcgag cacgcgcgat gcagagaatt cgtgaataaa ggccgatact ctagggttta
     1201 cccaggtaaa atgacgtggc aatgtgatca atcagaggca acgctcatgt ccaacgaaat
     1261 cctaatcgac gccgatccgt caccgtcgct tttaagcgtt actcacttgg tctacgcact
     1321 gcacgccctg agccttctga tcggcataac cactgcggcg acgatcatcg gagccttcgt
     1381 cttcggcctg ccgtcgatca tcgccgtggt tattaattac ttgaagcggg acgaggcgcg
     1441 cggcactttt ctcgagtcac acttccgctg gcagattcgg acgttttggt ttggcatgct
     1501 gtggtcgctg ctcggcggtt ttttattcat gactatcatt ggcattccgc tggctattgc
     1561 cgttttcatc ggcgccggcg tgtgggtgat ctaccgggtc gtgcgcggct ggttggcgct
     1621 gcgcgatcga aaaccaatgt atatcaattc ctgaccgagc tccgttgttg gcgatcaatc
     1681 gccgttaatt tgagagattc gtctcaattc cgaagcgaca atcaaattta ccgccttcat
     1741 gataccgacg ggattaagcg gatccctgac tcgattggca aatatttcat agtcggttac
     1801 cctctatcca aatgcgcgat cgtcgtgccg atctctgtgc cggccctttc gcgcgcggca
     1861 acccgacggt ttcgggatcg gcccgagcgg caatacggca tatgaaaacc gacgatctcc
     1921 gcatagttaa aactcatccg ctgctcgcgc cggcgatact cgccgaagag atccctctta
     1981 ccgaggctgc agcaggaaca gtctaccggt cgcgccgcgc catcgaggcg attctcgacg
     2041 gcaaggatgc ccggctcctg gtgatcgtcg ggccttgttc cattcacgac accaaggccg
     2101 cgctcgagta cgccactaat ctcaaggcga tcgcggatgc cctggccgag tcgctgctga
     2161 ttgtcatgcg tgtctacttc gaaaagccgc gcacggtggt aggctggaaa gggctcatca
     2221 acgatcctga tttagacgag tcgtatcaca ttaacaaagg cctcaggcta gcccgccaac
     2281 tgcttgccga tattctcgaa ctaggccttc ccgccggcac cgagtttctc gacaccacct
     2341 ttggtcagtt ctatgccgat ctgatcagct ggggcgcgat cggcgcgcgc accgccgaga
     2401 gtcaaatcca tcgagagcta gcatcgggtt tgtcgatgcc ggttggcatc aagaaccgca
     2461 cggacggtaa tgttcaggtc gccgtggacg cgattcaggc cgcccgctgt cgtcacctgt
     2521 ttccctctct gaccaaagaa ggggcgccag cgatcctgga aaccaccggc aatccttacg
     2581 cgcatctcgt gcttcgcggc ggcagcgaga gcggacctaa cttcgatacg gtttctctcg
     2641 atgctgctgt ccgcctgctg cgcgcagcag acctgccgga gatcgttatg gtcgactgca
     2701 gccacggcaa cagtgataag gacgccggcc ggcagcacga cgtcgccgca gcgatcatga
     2761 agaatctcgg aacgtcgccc gttcgagcgc tgatgatcga gagtcacctg gtcgccggcc
     2821 gccaaaacgc ccccattacc tatggtcaga gcattaccga tgcgtgcctg ggattcgacg
     2881 aaaccaaaga cttgctgcac cgactggccg acgccgtgcc taagtagaaa gagtcagaga
     2941 gattcatcgt aaactcaggt ggctcgtaaa gccgggcaga ccgacccatc ctgtttcaac
     3001 tgagacatct cctcgacatc gcgaattggc ttttataatg tcaggcgtga ccagctgcag
     3061 tgccggtccg ctgctcattg gccgaaagga ttgataatga aaaaaaatct tccgtcgata
     3121 atcgcgccgc tcatgatgag cgtgttactg cttgccggca agtccgttta cgctctcgac
     3181 aaagtcaatg tcactttacc gtccaaatcg tttcagttca ttatttttcc gcttgccaag
     3241 gagcgcggct atatgaggga ggaagggatc gatctcaata ccgtcatgat ggcctccact
     3301 ccgggtcttc aagccgtgct cgccggagaa atggatttca ccggttccgg cagtagtgcc
     3361 ctcgtcgctg tcagtcaagg caacgcgccg ctcaaaactg tgctggccat caccgatcag
     3421 gtgctgcagt ggctcatggt tcggccccag tacggtgctt tcaaagagct aaagaataaa
     3481 aaaatcgcag tcaccggcgt cgccgcggtg gcgaccttta tgttgaaaaa agtcgcgcca
     3541 aagtacggtc tcgacgccaa caaggatttg acctttctcg cactgccgcc gggccagcgc
     3601 ctcgctgcgt tgcagtcggg tgtcgtcgat ggcggcttgt tgagttcgga agaacgcttt
     3661 gccgccctcg accagggcat gaaagaaatc ttatacctcg gcaaagaagt gaaaaattcc
     3721 tggggtacca tcgcgaccaa cgatcaattc atcaaaacca agcccaagtt gatgcaggga
     3781 ttcacccgcg ctctgctcaa agccctgcgc ctggtcaagc aaaaccgtga ggtcgcgatg
     3841 gatgcgatga tgaaattttc cgagttggac agagatctag cagcgcgcac ctacgacggc
     3901 atgatcggaa cgttcacctc caatggcgtg gtcgacgacg aaacgcagaa aaacgatctc
     3961 gacattgttc gcgaggtcct caaagtaacc aaggacgtcc ccatcgagcg cgcctacgat
     4021 ttcagctttg ccaagaaagc cgacatggag ctgatccaat ccggctggaa gccttagaaa
     4081 aacgaccatc taatattatc accgttcgaa cacgcgctct tgggctgttc gtaactgctg
     4141 cacgtacaac tcagttaaat ctcccactac tttacaagcg ctttgtatta actagaatca
     4201 aacacacgct cgtcgcgttc tgccaacgaa aatagcggcc gcagggccgg cgtgatgagt
     4261 tggcgtggcg attataatca cgcgggtctt ggccgctctt gtccgagcac attcccaatt
     4321 ggcgcaacct tagccagtga ttcacgaacg gtattgctgc caccgataac ctattgaaaa
     4381 ggagatcagg cgatgccccc aacagtcgat aacaaatcgc tcaagaagct ccgctccatc
     4441 caacgcagcg ccgatcgcca ttgggtcggt aacggatttc cagttcggac actgttcgca
     4501 tactcgaatt tgggtgcggt tctcagtccg tttctactct tggattacgc cggaccgaaa
     4561 gaatttccgc cgacccaaga acgactcggc gttggcgagc atccgcaccg cggcttcgag
     4621 acggtaacca tcgtctacga cggcgaagtg gaacaccgcg actcttccgg cggcggaggg
     4681 cggattggtc ctggcgatgt gcagtggatg accgcggcat cgggcgtcgt ccatgaggag
     4741 tttcatgggc gcgatttcgc ccggcgcggc ggcatgtttg aaatggtgca actatgggtc
     4801 aatctgccgg cgcgcgataa actggcgcca ccgcgttatc agagcattac cagtagtcag
     4861 atacccgcca tccgtatgcc tggcggagag gcaacgatgc gtgtcatcgc gggggagttc
     4921 gcgggagcac agggaccggc gcagaccttt acgccaatcc tggtcggcga tctgcgcttc
     4981 tccaaagacc aacggacaga cctttccgtt gcccatggtc acaccaccgc gctggtggta
     5041 ctcaaaggcg ccgtgcgcgt gaacggaacc gatgagatca ccacagccga ggtcggcgtg
     5101 tttgagcgcg cgggcagaag catcagcatc gaatcggcca aagacacgac ggcattactg
     5161 ctttgcggtg agccgattaa tgagcccata gtcggccagg gaccgtttgt catgaacact
     5221 tctcaggaga ttcgccaggc gctcgcggat taccagagcg gcaagctggg tcacttggcg
     5281 taggagatcg gctggggacc atcatcaaac ggtcccgcac tcgccgcctg cccgatacta
     5341 agcagtacac ctaaaatgat cgttccaatt agtttgcgcg tggtcatcag gttatcgaag
     5401 aagctaaggg atcgggatca ccttttgaag tcaccgcaga ctactcgcgc accttgtgta
     5461 acttaacagc ttcgttgtac aggttgatta
//
LOCUS       DATLCH010000094        10408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10584833, whole genome shotgun sequence.
ACCESSION   DATLCH010000094 DATLCH010000000
VERSION     DATLCH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10584833"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..2083)
                     /locus_tag="VMZ02_02785"
     CDS             complement(269..2083)
                     /locus_tag="VMZ02_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70883.1"
                     /translation="MAQSGQQEDVIGKAYDLRLIRRLWQFVLPYRRMFLFTMLLLPLQ
                     QAFGLAQPYLMKIGIDRYIAAKDLWGLQNVMLLFLLALIGETVTVFGHLYFSMLVAQR
                     CLADLRVAVFSHVQKLPTSYFDRNPVGRLLTRMTTDVDVLQEMFSSGVLTLISDFIMV
                     AWIVGIMFYLHVGLALVSLALIPPMALAINFFRVKARQNYRQIRERIARVNAYLGEAI
                     SGMAVIQLFSREGKTYREFEALNADHRDAYHLSNSYEAALYSMVEAAGSVSLGLLLWY
                     GGGEVLHGVIGIGTVVAFKEYIHRFFVPLRDFSQKYAVMQSAMAAAERIFQMLDTPAD
                     IETGRSALVPKPFRGEVVFDNVWFHYKAGDPVLKGVSFRIEPGEKIAVVGATGSGKTT
                     TIKLLNRLYDIQQGSIKVSGVDVREWDLKALRQHIGVVLQDVFLFTGDIRTNLALGDP
                     SIAQERIENAARAANADTFIRQLPDTYHAPVRERGSNFSGGQRQLLSLARVLVFRPEI
                     LVMDEATSSVDTETEALIQDALEKVMRNRTCLLIAHRLSTIRNADRIIVLHHGEVREM
                     GSHAELMEQQGIYHRLYQLQYEREELKLSSHVESKVIA"
     gene            complement(2083..3822)
                     /locus_tag="VMZ02_02790"
     CDS             complement(2083..3822)
                     /locus_tag="VMZ02_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70884.1"
                     /translation="MGRLWQYLRRYWKRYLVGGLCLLATATLVMMIPWWTRDVVRIIE
                     RGGSLNDVTFYALLIICAAIAQGIVRTYSRALIFNAGRDVEYDLRNDLFAHLEKLPLA
                     FYQSQRTGDLMSRVINDISAVRVMLGPGVLNFVNAPVYYIYALVLMLSMDVRMTVAAL
                     APFPLLMYFARRFRGRIMKSSLEVQQQMSVLSSHVQENLSGMHVVKAYSQEDYQTQQF
                     IALNKDYEVKSMEMAKMRGIVNPVMQGVNALTVLIVIWYGGVRVVRGDLLVADIVAFI
                     AYLNVLAWPTAAFGWMLSLVERGRAAMHRLEEVLETKPAITDGSESVSVDRLKVAIEF
                     RNVSFAYERRQNGHAALDGISFKLPVGQSVGLVGRIGSGKSTVAQLMPRLFDVASGEI
                     LFDGQDIRKFSLSQLRRMIGYVPQDPFLFSTSLRRNLALGREEYSEEELTRAIRLAKL
                     DRDLDVFPQGLETMVGERGVTLSGGQKQRATLARALIMDPPVLVLDDSLSSVDAQTEA
                     EILHGLRSLLKEKTCLIISHRISAVKEADEILVLDEGRIIERGNHAGLVRQGGVYAEL
                     YQQQRLSEELEQI"
     gene            3946..5781
                     /gene="typA"
                     /locus_tag="VMZ02_02795"
     CDS             3946..5781
                     /gene="typA"
                     /locus_tag="VMZ02_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HUR70885.1"
                     /translation="MTQAQLSRRDDLRNIAIIAHVDHGKTTLVDALLHQSGIFRANEQ
                     VAERVMDSMDLERERGITIMAKNTTIHFANVKINIVDTPGHADFGGEVERTLAMVDGV
                     MLLVDASEGPLPQTRFVLKKALEAKLTPIVCINKIDRPDARIAEVLDEIYDLFIDLDA
                     SEDQLDFPVVYTNARAGTATLDLKQSGQNLKPMFELIVNTLPGPSYDPAMPTQFQANN
                     LDYNDYVGRLAVGRVKNGSLTPGQFMLCRVGGKQELVKITQVYGWQGLKRVEVSSADA
                     GDIAAIAGIEDIGIGDTITDREKPQALPPLRIDEPTIAMLFIANNSPWSGREGEHVTS
                     RKLRERLFFEARRNVSLRVEETATADAFQVTGRGEFALAIVIETMRREGYEMMVSKPT
                     VVTREIDGVIHEPMELLVIDIPEDFIGVVSQLLAMRKGKMTKMIHAGTSRVRLEFAVP
                     SRGLIGFRSRFLTDTRGTGIMNALFNGWEPWHGPIQSRTNAAMVADREGSAPPYAIYH
                     LQERGVMFIAPGTKVYEGMVVGEYSRENDLNVNVCKEKKLTNMRAAGKDENIIISPHR
                     EMGLEAALEWIADDELVEVTPQSIRLRKKILKQTDRPRRRHEQED"
     gene            complement(5784..6185)
                     /locus_tag="VMZ02_02800"
     CDS             complement(5784..6185)
                     /locus_tag="VMZ02_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70886.1"
                     /translation="MRTANFALALWAGVLSTSIGVFAAQPAKTPAAKEQPNKTPPADD
                     KNVTWKLAGREGECTPLDILGKKGPAYGDVKSPQELADKLKANGHDAEVKEFKAGIRP
                     AVEVRAPSAGIHVMFVRQEHCDKKLPGVEKR"
     gene            complement(6554..6799)
                     /locus_tag="VMZ02_02805"
     CDS             complement(6554..6799)
                     /locus_tag="VMZ02_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HUR70887.1"
                     /translation="MKPKLTLYSRRECCLCDEMKAVIRQVAIFQEFDFEDVDIDASRE
                     LEERYNEEVPVLFIDGRKAFKYRVTAVQLAKRINKKR"
     gene            complement(6796..9915)
                     /locus_tag="VMZ02_02810"
     CDS             complement(6796..9915)
                     /locus_tag="VMZ02_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HUR70888.1"
                     /translation="MRKLLKIGVFIGITGVLFFGVCTIAFYHLVSVGEFRTFLVEEIE
                     KNTALKVGVGAAELEIGWVTGVGFRDVAIAMPESTMPEIAAERVTARVALLPLLHRKI
                     IFYEIRLQRPTVALVVNTEGRLPLLDKLLNLPLLKRDNSDFSLDLQSIKISKGALGYV
                     DRRPTSSVGEWRLRDAEADIERVRGQKLREFMKDLMARQTGEPTGAALHFAMKGKVEK
                     DNLPVQFNASGRVVFPQEALVLSEARWHADIDLVDIPAALIKAQFSVHLPIKSVSGHL
                     AQRLHLEGYPGKQLRLQGDVEFKQLSLDAPELLTTPLASANGRTTFEAEWSRGQLVIR
                     RADSRIDDVKFTLAGSLTALESAEPHLALRLSGLTGPLTSLRRYLPSRIIDSPVWEKS
                     LSSLQTGQVELKAAGVNATLGELRRLAQTGISKQLWFDAEFHDVSGNLAAQGTLPLRG
                     VSGRVSLKDGRLSVRDLKGGYGDSQLHEVDGSYDLTPSGRGGLDLTARGDFNLPELRD
                     QLKAEFISDRAAKIAASINELDGRSRVELVLKRVANEPLQFAGKIALDNARMRYDDYA
                     LSEIKGEIAFSPHEIRGEKIRAQLSGSPVQLQLTLKDYASEGGTFDLGVESSGVRTGV
                     ITRLLLSQGTLNDPGIIRGAVRYRGAFADKNTHRFTGDLDLTNVYLMMKPLLQPLREL
                     SGKIKINDTGIDFQNVKGLLVGAPANFSGRWRYAEKPPLLFDFAAPNLDVTYLISQID
                     AEGSEFYAKLQAEGKITLAKGRIKNFEFNDMKSDTTIDRRVWRLTNLNARSAGGTIVG
                     ATTIFDKPDSLGLVAEPRIQGVPVQSFLKWFDLTNTEMTGQVNLTGKLETVGNSDPER
                     KQNLSGAFSLRIEEGTINRMRVLVQLLNLLDLSRWFTLQLPDLTKQGIRFRAITADFK
                     VVKGVYSTENLVVDSGDLRMTGVGKIDVPRDDLDLVVAVRPFAGIDSAINQIPILGRG
                     IAAIKNSFLVASFNIKGKIQDPTITPAPLGTLSEMFWSVLGIPKNIIGLGEGEKKEEA
                     TEAVKTTPK"
     gene            10174..10371
                     /locus_tag="VMZ02_02815"
     CDS             10174..10371
                     /locus_tag="VMZ02_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR70889.1"
                     /translation="MVDRENEELLNSKQVAVILDMSPDTVNEFARKNILPAFKKGKQW
                     RFRKRDISIFYKKQPKDIRDA"
BASE COUNT         2423 a         2990 c         2785 g         2210 t
ORIGIN      
        1 atactcgcgc accgccaaaa cggttttcaa tttctctttg gtgtcggagt tgacgtcggg
       61 gtgctggatg tacgccgcga tcggttggcg tcgccagaga atttttcctt cttcgtacgc
      121 ggcacgcaga acgtagacgg gagaacagcc cgaaagcaaa gacatcagcg caactgccga
      181 taatccgatt ccaatggact gaaacgatgc cattcctttt agacgaacaa agtacagcga
      241 ttgctcgacg gcagcaccga cgaactggct aggcaatgac cttggattcg acatggctgc
      301 ttagcttcaa ctcttcgcgc tcgtactgca gttggtacag ccggtgataa atgccttgct
      361 gctccatcag ctcggcatgg gaccccatct cgcgcacttc accgtggtgc agcacaataa
      421 tgcggtcggc gttgcggatg gtcgacagcc gatgcgcaat caacagacag gtgcggttgc
      481 gcattacttt ctccagggcg tcttggatca atgcttcggt ctcggtgtcg acactggaag
      541 ttgcctcgtc catcaccaaa atttccggac gaaacaccaa tacgcgcgcc agcgacaaga
      601 gctggcgctg accgccggaa aaattgctgc cgcgttcgcg cacaggagca tgataggtgt
      661 cgggcagctg gcggatgaag gtgtccgcat tagcggcacg ggccgcgttc tcgatgcgct
      721 cctgggcaat cgacgggtcg cccaatgcca aattcgtgcg tatgtcgccg gtgaataaaa
      781 atacgtcttg cagtaccacg ccgatatgtt ggcgcaacgc tttcaaatcc cactcgcgga
      841 catcgacccc gcttactttg atcgatccct gctgaatatc atagagccta ttgagcagtt
      901 tgatggtcgt cgtcttgccc gaacccgtgg caccgacgac ggcgatcttt tcacccggtt
      961 cgatgcgaaa cgacacgcct ttaagcactg gatcgccggc tttgtagtga aaccaaacat
     1021 tatcgaaaac cacttcgcca cgaaacggtt tgggcaccaa cgcgctcctg ccggtttcaa
     1081 tgtcagccgg ggtgtcgagc atctgaaata tacgttccgc cgccgccatg gcggactgca
     1141 tcaccgcgta cttctgactg aagtcgcgca gcggcacgaa aaatcgatgg atatactcct
     1201 tgaacgccac cactgtgcca atgccgataa ccccgtgcaa aacctcgccg ccaccgtacc
     1261 agagcaacag acccaagctt accgagcccg ccgcttcgac catcgaatag agcgccgctt
     1321 cgtaggaatt cgacaagtgg taggcgtccc ggtgatcggc gttgagcgct tcaaattcgc
     1381 ggtaggtctt gccctcgcgc gaaaacagct ggatgaccgc catgccggaa atcgcctcgc
     1441 ctagataggc gttgactcgc gcgattcgct cgcggatctg gcggtaattt tgccgcgcct
     1501 tgacgcggaa aaaattgatc gccaaagcca tcggtggaat cagcgccaaa gaaaccagcg
     1561 ccaagccgac atgaagataa aacataatgc cgacgatcca cgcgaccatg atgaaatcgg
     1621 aaattagggt caagacgccg gacgaaaaca tttcttgcag cacgtccaca tcggtagtca
     1681 tgcgcgtcag cagccggccc accggattgc ggtcgaaata actcgtgggc agtttttgca
     1741 catgggaaaa caccgcaacc cgcagatccg ccagacaacg ctgcgccacc aacatggaaa
     1801 aataaagatg cccgaatact gtcaccgtct cgccgatcaa ggcgaggaga aacagcagca
     1861 tgacattttg cagtccccaa agatccttgg cggcgatata gcgatcgatg ccaattttca
     1921 tcaggtaggg ttgcgccaag ccaaacgcct gctgtaacgg cagcagcagc atggtgaaaa
     1981 gaaacattcg ccggtaggga aggacgaact gccacaagcg ccgaatcaag cgcaaatcgt
     2041 aggctttgcc gatgacgtct tcttgctgtc ccgactgcgc cattagattt gttcgagctc
     2101 ctcgctcaag cgctgctgct gataaagctc ggcgtatacg cctccttgtc gcaccaggcc
     2161 ggcgtgattg ccgcgctcga tgatcctgcc ttcgtcgagg acgaggattt catcggcctc
     2221 tttcaccgcc gagatacgat gggagatgat caagcaggtt ttctctttta aaagagaccg
     2281 caagccgtgg agaatctcgg cttcggtttg ggcatcaacg ctggaaagac tatcgtcgag
     2341 aaccaatacc ggcggatcca tgatcagcgc acgcgccaag gtggcccgct gcttttgccc
     2401 gcccgagagc gtcactcccc gttcaccgac catcgtctct aagccttggg gaaatacatc
     2461 caaatcgcgg tcgagtttgg caagtctgat ggcacgagta agttcttctt cggaatattc
     2521 ttcccggccc aacgccagat tgcgccgcag cgaagttgaa aaaagaaatg gatcctgcgg
     2581 aacgtaacct atcattcggc gcaactgaga tagggaaaat ttgcggatgt cctgcccgtc
     2641 aaaaagaatt tcgccggaag cgacatcgaa cagccgcggc atgagctgcg ccaccgtact
     2701 cttgccggag ccgattctcc cgaccagccc aaccgattgg ccgaccggca gtttaaaact
     2761 gatgccgtcg agagcagcat ggccgttttg cctgcgctca taggcaaacg atacgttgcg
     2821 aaactcaatg gctaccttga gtcgatcgac gctgaccgac tcgctaccat ccgtgatggc
     2881 gggtttggtt tcgaggacct cttcgagccg atgcatcgcc gcccggccgc gctcgactag
     2941 cgatagcatc caaccaaacg ccgcggtggg ccaggccaat acgttcagat aggcaatgaa
     3001 ggcaacgata tcggcaacca acaagtcgcc acgcacgact cgcacaccgc cgtaccagat
     3061 aacgatgagt acggttagcg cattgacacc ctgcatgacc gggttgacga tgccgcgcat
     3121 cttggccatc tccatgcttt tgacttcgta gtccttgttg agagcgatga actgctgggt
     3181 ctggtaatct tcctgcgagt aggccttgac gacgtgcatg ccgctaagat tttcttggac
     3241 atggctgctc agcaccgaca tttgttgctg cacttcgagg gaactcttca taatccggcc
     3301 gcggaagcgg cgcgcaaagt acatcagcag tggaaatggc gccagcgcag ccacggtcat
     3361 gcgcacgtcc atcgacagca tcaataccaa cgcataaatg tagtagaccg gcgcattaac
     3421 gaagttgagc acaccagggc cgagcatgac gcgcacggca gagatgtcgt tgatcacccg
     3481 cgacatcaaa tcgccggtgc gctgcgattg ataaaacgcc agcggtaatt tttccaggtg
     3541 ggcgaaaaga tcattgcgca gatcgtactc gacatcgcgt ccggcgttga aaatcaacgc
     3601 ccgcgaatag gttcgcacga tcccttgggc aatagcagcg cagatgatca acagcgcgta
     3661 gaaagtgacg tcgttaagcg acccgccacg ctcaataatg cggacgacgt cgcgagtcca
     3721 ccaaggaatc atcatgacca gagtcgcggt cgccaacaag cacaacccac cgaccaggta
     3781 gcgtttccaa taacgccgga gatattgcca cagccgaccc atgaaaattc aaaaatacgc
     3841 tacggcactg tcaatcgcaa ggtaacacct tccaagggtt gagatttgcg gcgcttttca
     3901 tcgtgagctt gacttcgcct ggtcgaactt taataacaga gggtcatgac tcaagcccaa
     3961 ctctcgcgcc gcgacgacct gcgcaacatc gcgatcatcg cgcatgtcga tcatggcaaa
     4021 accactttgg tggacgcctt actgcaccag agcggtattt tccgcgccaa cgaacaggtc
     4081 gccgaacgcg tcatggattc catggacctg gaacgcgaac gcggcatcac cataatggcg
     4141 aaaaacacca cgatccattt cgccaacgtc aaaatcaata tcgtcgacac tccggggcat
     4201 gccgatttcg gcggcgaagt cgaacggact ttagcgatgg ttgacggagt catgctactg
     4261 gtcgacgcct cggaaggccc gctgccgcag acccgcttcg tcttgaagaa agcgcttgaa
     4321 gccaagctca caccgatcgt ctgcattaac aagattgacc ggcccgatgc gcgaatcgcc
     4381 gaagtgctcg atgaaatcta cgatctattc attgatcttg atgccagcga agaccagctc
     4441 gactttccgg tcgtttatac caatgcccgc gccggcaccg cgactttgga tttgaaacag
     4501 tccggtcaaa atctgaaacc gatgttcgag cttatcgtca acacattgcc aggaccaagc
     4561 tacgacccgg caatgccgac gcaatttcaa gccaacaacc tcgattacaa cgattatgtc
     4621 ggccggctcg cggttgggcg ggtgaaaaat gggtcgctga cgcctgggca attcatgctc
     4681 tgccgcgtcg gcggcaaaca agaattagtc aagatcaccc aagtctacgg ctggcaaggg
     4741 ctcaagcgcg tcgaagtctc gtcggcggat gccggcgata tcgccgccat cgccggcatc
     4801 gaagatatcg gcatcggcga caccatcacc gatcgtgaga aaccgcaggc gctgccgccg
     4861 ctgcgcatcg acgagccgac cattgcgatg ctgttcatcg ccaacaattc gccatggtcc
     4921 ggccgcgaag gcgaacatgt tacctcgcgc aagttgcgcg aacggttgtt tttcgaggcc
     4981 cggcgcaacg tcagcctgcg cgtcgaagag actgcgacag ccgatgcttt tcaggtcacc
     5041 ggccgcggcg aatttgcctt ggcgatcgtc atcgaaacta tgcgccgcga aggctacgaa
     5101 atgatggtct ccaaaccgac cgtcgtcacc cgcgaaattg acggcgtcat tcacgaacca
     5161 atggaactct tggtgatcga tatccctgag gattttatcg gcgtcgtgtc gcagctactt
     5221 gccatgcgca agggcaaaat gacaaaaatg atccacgccg gcacgagccg ggtgcgtttg
     5281 gaatttgccg tgccgtcacg cggcttgatc ggttttcgct cgcgctttct caccgacacc
     5341 aggggcactg gcatcatgaa tgcgttattt aacggctggg agccctggca tgggccaatt
     5401 caatcgcgca ccaacgcagc catggtcgcc gaccgcgagg gctcagcgcc gccctacgcg
     5461 atctatcact tgcaagaacg cggcgtcatg tttatcgcgc cgggcaccaa agtatacgaa
     5521 ggcatggtgg tcggcgagta ctcgcgtgag aacgatctaa acgtcaacgt ttgtaaagaa
     5581 aaaaagctca cgaacatgcg cgctgccggc aaagacgaga acatcatcat ctcgccgcac
     5641 cgggagatgg gcttggaagc cgccttagag tggatcgctg acgatgagct ggtggaagtc
     5701 acgccgcaat cgattcgact gcgcaagaaa attctcaagc agaccgatcg cccacgccgg
     5761 cgtcatgaac aagaggattg aacctacctt ttctcgaccc caggcagctt cttgtcgcag
     5821 tgctcttggc gcacgaacat aacgtgaatc ccggctgacg gcgcgcgcac ctcgaccgcc
     5881 ggacggatac cggctttgaa ttctttgacc tcggcgtcgt gaccgtttgc cttgagctta
     5941 tcggccagct cttgcgggct cttaacgtcg ccgtaagccg gtcctttttt gccgagaatg
     6001 tcgagcggcg tgcactcgcc ttcgcgcccg gccaatttcc acgtaacgtt cttatcatcg
     6061 gcaggcggcg tcttgttcgg ctgttcttta gccgcaggtg ttttagccgg ttgtgcggca
     6121 aacacgccaa tggaagtgga caatacccct gcccatagcg ccaaggcaaa attagctgtt
     6181 ctcatgacgt gccccccaac agaagagtaa atttaaagaa gggcgagcgc aacttggtgg
     6241 cccgggagac aacgcatggc ggtggttacg caactgactc tttctgcgct ccgttcccac
     6301 gccaaaaaat agctctgcgg ccgatccaac gctgcgttaa agcggatcaa ggcggcaact
     6361 tctgtccgcg tggtgtttca ttcagaagtg cccggagcac atttggagac gccattcgat
     6421 cgccgtgaga agactttgat ctgcgcattc cgagcgtcgg cgaacttcga accacgtgca
     6481 gaggtgtcgg agatttgctc tgggcgttca agttgagccg attcccatca gagtcgcgaa
     6541 gagcgttaaa atttcatctt tttttattga ttcgttttgc cagctgtacg gcggtgactc
     6601 ggtatttgaa cgccttccgt ccgtcaatga acaagaccgg cacctcttcg ttatagcgct
     6661 cctcaagttc acggctggca tcgatgtcga catcttcgaa gtcgaactct tgaaaaatcg
     6721 cgacttgccg aatcacagct ttcatctcat cgcacagaca gcactcccgg cgcgagtaga
     6781 gtgtcagttt gggtttcatt taggcgtcgt cttgaccgcc tcggtggcct cttccttctt
     6841 ctcgccctca cctaatccaa tgatattttt aggaatcccg agtacgctcc agaacatctc
     6901 cgaaagagta ccaagcggcg cgggcgtgat cgttggatct tggatcttgc ccttgatgtt
     6961 gaagctggcg acgaggaagg aatttttgat ggcggcaatt ccccggccga gaatggggat
     7021 ctggttgatc gccgaatcga tcccggcaaa ggggcgcacg gcgaccacta gatccaaatc
     7081 gtctcggggc acgtcgattt taccgactcc ggtcatgcgc agatcgccgc tatcgacgac
     7141 aagattctct gtcgagtaga cgcctttgac gactttgaaa tcagcggtga tagcgcgaaa
     7201 tcgaatccct tgcttggtca ggtcaggcaa ttgcaaagta aaccaacgcg atagatcgag
     7261 caggttaagc aactgcacca ggacacgcat acggttgatc gtgccttcct caatgcgcaa
     7321 gctgaacgcg ccggacaagt tctgtttgcg ttcggggtcg gagttgccga cagtctcgag
     7381 cttgccggta agattgacct ggccggtcat ttcggtgttg gtgagatcga accatttgag
     7441 aaacgactgc acaggaacgc cttgaatcct cggttcggca accagcccta gactatccgg
     7501 cttatcgaaa atcgtggttg cgccgacgat cgtgccgccg gcggagcgcg cgttcagatt
     7561 ggtcaagcgc cagacacgcc ggtcgatggt cgtatcgctc ttcatatcat tgaactcaaa
     7621 gttcttgatc cgccctttgg caagagtaat tttcccctcg gcttgaagtt tcgcataaaa
     7681 ttcgctgccc tcggcatcga tctgcgagat caaataggtc acgtccaaat tgggcgcggc
     7741 aaagtcgaac agcagtggtg gtttttccgc atagcgccat cggccgctga aattggccgg
     7801 cgcgcctacc agcagcccct tgacgttttg gaagtcgatg ccggtgtcgt tgattttaat
     7861 cttgccgctg agttcgcgca gcggctgcaa aagcggtttc atcatcaagt acacattggt
     7921 tagatcgaga tcgccggtga atctatgagt gtttttgtcg gcgaaagcgc cgcgatagcg
     7981 caccgccccg cggatgatac cgggatcgtt aagagtgcct tgagacagca agaggcgcgt
     8041 tatcacaccg gtgcgcaccc cggatgattc gacacccaga tcgaaggtgc cgccctcgct
     8101 ggcatagtct ttaagagtca attgcagctg caccggcgag ccgctcagtt gcgcgcgaat
     8161 tttctcgccc ctgatttcgt gcggcgaaaa ggctatttct cccttgatct cgctcaacgc
     8221 ataatcatcg tagcgcatcc gcgcattgtc caaagcgatt tttcccgcga actgcagcgg
     8281 ctcattggca acgcgcttta ggaccaactc gacacggctg cggccgtcaa gttcattaat
     8341 cgatgcggcg attttggcag cacggtcgga aataaactcg gctttgagct ggtcgcgcag
     8401 ttcgggcaaa ttaaaatcgc cgcgggccgt gagatcgaga ccgccgcgtc cactcggggt
     8461 taaatcatag ctgccatcga cttcgtgcag ctgggaatca ccgtaaccgc cttttagatc
     8521 gcgcaccgac aaacggccgt ctttcaaact cacgcggccc gaaacgccgc gcagcggcaa
     8581 tgtaccctgc gctgcaagat tacccgagac gtcatgaaat tccgcgtcga accacagctg
     8641 tttactaata cccgtctggg ccagccggcg cagttcaccc aacgtggcgt tgactccggc
     8701 agccttgagt tcgacctgcc cggtttgcag cgagctcaag ctcttttccc acaccggcga
     8761 atcaatgatt cttgatggca agtagcggcg cagagacgtc agtggcccag tcagccccga
     8821 cagccgtagc gctaaatgcg gctcggcgct ctcgagggca gtgagactgc cggccaatgt
     8881 gaatttgaca tcgtctatgc gcgaatcggc gcgtcttatg actaactgtc cccggctcca
     8941 ctccgcttca aaggtcgtcc gaccattcgc gctagccaat ggggtggtca gcaattcggg
     9001 tgcatctaag gaaagttgtt tgaactcgac atcgccctgg agtcgcagct gcttaccggg
     9061 ataaccttcc aagtggaggc gctgggccaa gtggccgctc accgacttga tcggcaagtg
     9121 gacgctgaac tgggctttta tcagcgctgc aggaatatcg acaagatcaa tgtcggcgtg
     9181 ccaccgcgct tcgctcaaca ccagagcttc ctgagggaaa accacccgtc ctgacgcgtt
     9241 gaactgcacc ggcaaattgt ctttttcaac tttacccttc atcgcgaaat ggagggcggc
     9301 accggtcggt tctcccgtct gccgtgccat cagatctttc atgaattcgc gcaacttctg
     9361 accgcgcact cgctcaatat cggcttcggc atcgcgcaag cgccactcac ctacgctgct
     9421 ggttgggcgc cggtcgacat aaccgagcgc acccttcgat attttgatcg actgcaaatc
     9481 tagactgaag tccgaattgt cgcgtttgag cagcggtaag ttaagcaact tgtcgagcag
     9541 cggcaggcga ccttcggtgt ttacgaccag agccacggtc ggccgctgca aacggatttc
     9601 atagaaaatg attttgcggt gaagtaacgg cagcaatgct actcgagccg tcactcgctc
     9661 ggcggcgatt tccggcattg tactctccgg catcgcgatc gccacatcgc gaaaacctac
     9721 tcccgtaacc caaccgatct caagttccgc tgcgccgacg cccaccttca gtgcggtatt
     9781 tttttcgatc tcctcgacaa gaaaagtgcg aaattcgccg acgctaacca gatggtaaaa
     9841 tgcaattgta caaacgccaa aaaaaagcac cccggtgatg ccgatgaata cgccaatctt
     9901 gaggagcttt ctcatgtcga gtcagataca tgctacaagg cttcgagcag ataccctcta
     9961 cggtgggaat gcaacgaatt aaaccgtcgt catgctaaat gaagttcgcc gttgtcgcaa
    10021 gcgttttgca acgccgagcg atcccctcag cgcctcagcg aaatcgcata agtaccggga
    10081 agcactaggc gattcttgcc ttgacagccc gggatgaact tgttagataa aacgaacttt
    10141 ttcgcctact ggagtttact gcacggagag tctatggtgg acagagagaa tgaagaactg
    10201 ctcaacagca aacaagtcgc cgtgattctc gacatgagtc cggacactgt caatgaattt
    10261 gctcgaaaaa acatccttcc ggccttcaaa aaaggcaagc aatggcgatt tcgcaagcgc
    10321 gacatttcga ttttttacaa aaaacaaccg aaggatatcc gcgacgccta gtcggacgtg
    10381 gttgattggg gttggggcgc atggctga
//
LOCUS       DATLCH010000095         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_302823, whole genome shotgun sequence.
ACCESSION   DATLCH010000095 DATLCH010000000
VERSION     DATLCH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302823"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VMZ02_02820"
     CDS             <1..216
                     /locus_tag="VMZ02_02820"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70890.1"
                     /translation="MRNLVLKNQVLFGTVNAGRDTFETAIRDLQIFSNAWPEAVKSLI
                     TGRFAMTDHRELLLGKPTGIKNVIQLN"
     gene            306..1088
                     /locus_tag="VMZ02_02825"
     CDS             306..1088
                     /locus_tag="VMZ02_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HUR70891.1"
                     /translation="MTEALKHHWPEYLIEAVSLGVFMISAFSFATLLEHPSSPVHQAI
                     ANPTLRRFLMGLAMGSTAIAIIYSPWGQQSGAHINPSTTLTFFWLGKIPIWDTVFYVA
                     GQFLGGTAGALGVSFLLHPRAAHASINYVVTMPGAWGSPGAFAAEIIITFVLMMVVLH
                     VSNHSKLHKFTGLCAGGLVAAYITIEAPVSGMSMNPARSFASAFAGNSWSSLWVYFTA
                     PLIGMLAAAEVYVRRTDLSHVSCAKLHHDNAKRCIFCGKPAT"
     gene            1131..1748
                     /locus_tag="VMZ02_02830"
     CDS             1131..1748
                     /locus_tag="VMZ02_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR70892.1"
                     /translation="MTVQQLGFPACVAALIYLTACSGVSTDVAAGRNALQTGRPNDAV
                     GYLRRAADLDPDYKTPDRVPERVLTYLGRAYYETGDDAAARQTLEKAVTMHRDDPLAH
                     LYLGLALLRGGERERGRKEIEEGLKAIVDTLDYIAEDRVYGFYWDPGMTIRNDVKKTL
                     APMLNDNDLALAAQYIGREFDAEIDRARRDETRSRFSGSGGDGGS"
     gene            2022..2642
                     /locus_tag="VMZ02_02835"
     CDS             2022..2642
                     /locus_tag="VMZ02_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70893.1"
                     /translation="MTEDPEVLAWNNCHPDDLWIFDKLLLSKKFNYLCGPGSVPVPTA
                     NFYIVRPVINLYGMGVGAEVKWLTPADSTAVPPGYFWCEEFKGRHLSIDYDRHGVQLN
                     CVEGIRRVNSPLYKFSRWKMVNLAVEFPEVLKTLVGSYEFYNVEIIGDKIIEVHLRQN
                     HDPIQNDLQVCWEKDKKHESLRFLPQHEDADGYLPEKRLGFYISKR"
     gene            complement(2967..>3286)
                     /locus_tag="VMZ02_02840"
     CDS             complement(2967..>3286)
                     /locus_tag="VMZ02_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HUR70894.1"
                     /translation="GFGGEIFVITSVYLVMPVGRLSLRHALIGGITAAVLWELTRHVL
                     VWYFATLSQIGVVYGSLTTAIAVLLSLDLAATLLLLGAQVISEYERVDRVKASAAPQP
                     MRT"
BASE COUNT          775 a          917 c          871 g          723 t
ORIGIN      
        1 atgcgcaatc ttgttttgaa gaaccaggtg ctcttcggca ccgtcaacgc cggccgcgac
       61 actttcgaaa ccgcgattcg cgatctccag atattcagca atgcatggcc cgaggcggtc
      121 aaatcgttga tcaccggccg ctttgccatg accgatcatc gcgaactatt gttgggaaaa
      181 cccaccggca tcaaaaatgt catccagcta aattaatctc gtgcgctcgg taatgacgag
      241 cgagcactag cccgatcaca aacacgaccc gcgcacttcc catgctaccg cggcattcat
      301 tgaaaatgac cgaagctctc aaacaccact ggcccgaata cttgatcgaa gctgtgtccc
      361 taggtgtatt catgatctcg gcgttcagct tcgccacact gctcgagcat ccgtcctcgc
      421 ctgtacacca agcgatcgct aacccgacgc tgcgccgttt cctcatgggt ctggcgatgg
      481 gtagcacggc catcgcgatt atctattcac cgtggggcca acaatccggc gcgcacatca
      541 atccatcgac gacgctaact tttttttggc tcggcaagat tcccatctgg gacaccgtct
      601 tctacgtcgc cgggcaattc ctcggcggca cagccggcgc cctcggcgta tcgtttttgc
      661 tccatccgcg ggcggcgcat gcgtcgatca attacgtcgt taccatgccg ggcgcgtggg
      721 gtagtccagg cgcgttcgcc gccgaaataa ttattacctt tgttctcatg atggtggtat
      781 tgcatgtgtc gaaccactcc aagctgcata aatttaccgg cctgtgcgcc ggcggcctgg
      841 tagctgcgta cattacgatc gaagcgccgg tgtccggcat gagcatgaat cccgcgcgct
      901 cttttgcctc ggcgttcgcc gggaactcat ggagcagtct gtgggtttat ttcactgcac
      961 cattgatcgg tatgctagcc gctgccgaag tctacgttcg aagaaccgat ctctcacatg
     1021 tgagctgtgc caagttgcac catgacaatg ccaagcgctg cattttttgc ggcaaaccag
     1081 caacgtaaaa acaaccagag tcggaagctc cggacaaaga ggtaaatgcc atgactgtac
     1141 aacaacttgg attccccgcc tgcgtggcgg cgctcatcta cttgacggct tgcagcggtg
     1201 tcagcaccga tgtcgctgcc ggacgcaatg cgttgcagac cggccggccc aatgacgcgg
     1261 tcggctactt gaggcgcgcg gcggatttgg accccgatta caaaacccct gaccgcgtgc
     1321 cggagcgggt gctgacttat ttgggccggg catactatga gaccggcgat gacgccgctg
     1381 cgcgccagac cctggaaaag gccgtcacca tgcatcgaga cgatccgctg gcgcatcttt
     1441 atcttggttt ggcgttgctc cgcggcggcg aacgcgagcg cggccgcaag gaaatcgaag
     1501 aaggcctcaa agcgatcgtc gacacgctcg attacatcgc cgaggaccgg gtttatggct
     1561 tctactggga cccgggcatg accattcgca atgacgtgaa gaaaaccctc gcgccaatgc
     1621 tcaatgacaa cgacttggcg ctagccgccc aatatatcgg ccgggaattc gatgctgaaa
     1681 tcgatcgcgc ccggcgcgac gaaacgcgca gtcgtttcag cggcagcggt ggtgacggcg
     1741 gcagctgatc gctgcaccga acaccgaaca cttttcttgt atcggccgat aagacctgaa
     1801 cacgcaacgc gcataagatt tcgttccgca gaggacatat aaataaagat ttcatgcctc
     1861 tagcttgcct cggaaattgc gaaagcagag caaataattt catcttgtaa gtccgccctg
     1921 cggtgctatc gttttgtctt atggcggaac gaagcttgac tcgcagcgcg acaccccaag
     1981 ccaccgcaga gccgctttct ccatctaggg acgagcgact cgtgactgaa gatcccgagg
     2041 ttcttgcatg gaataattgc catcccgatg atctttggat ttttgacaag ttattgttga
     2101 gcaaaaaatt caactatctg tgcggtccag gatccgttcc cgtacccacg gctaattttt
     2161 acattgtcag accggttatc aatctatacg gaatgggcgt cggcgcggag gttaagtggt
     2221 tgacgccggc cgattccact gctgtgcctc ctggttattt ttggtgtgag gagtttaaag
     2281 gccggcatct gagtatcgat tacgaccgcc acggcgtgca acttaactgc gtcgaaggca
     2341 tccgccgcgt gaattcacct ttgtataaat tttctcgatg gaagatggtg aacctggccg
     2401 tagagtttcc cgaagtactg aaaactctgg ttggatcgta cgagttttac aacgtcgaga
     2461 ttattggcga caaaatcatt gaggttcatt taaggcaaaa tcatgatccg attcaaaacg
     2521 atctccaagt ttgttgggaa aaagataaaa agcatgaaag cctgagattc ttgccgcagc
     2581 atgaagatgc cgatggctat ttaccggaaa aaagactcgg cttctatata tccaagcgat
     2641 gaaatagccc gcccatgcct ggaaaatgca cgcagtgtgg cgccacgacc tacttgggcc
     2701 ggacaaccat acgcaccgat ctactagaaa tacgtaacgt gccgtgcaca ctgtgccagg
     2761 aatgcgacca tgagcagatt ggttcgcagg cacagagtag aatcaacaag ctcgtggagc
     2821 gagcagccaa gggaaaacta aaggatcgag tggtgaccct gtagcgatta cagcatcaat
     2881 gatttttggc cgtttattcc gcaggagcac tgaaggacgc aactaatagt ttgcagattc
     2941 acccagcgca gttgaaacca acttatttag gtgcgcatcg gctggggcgc tgcactcgcc
     3001 ttgactctgt cgacgcgctc gtattctgaa atgacctggg cgccgagcaa tagcaacgtc
     3061 gctgcgagat ccaagctcag tagtaccgcg attgccgtcg tcaatgagcc gtagaccaca
     3121 ccgatctgtg acagggtcgc aaagtaccag acgagcacat ggcgggtgag ttcccaaagc
     3181 accgccgccg tgatgccgcc gatcagcgcg tggcgcagcg acagccgtcc cacgggcatg
     3241 accagataga ctgatgtgat aacgaaaatt tctccgccaa atccga
//
LOCUS       DATLCH010000096         8838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5526536, whole genome shotgun sequence.
ACCESSION   DATLCH010000096 DATLCH010000000
VERSION     DATLCH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5526536"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1102)
                     /locus_tag="VMZ02_02845"
     CDS             complement(224..1102)
                     /locus_tag="VMZ02_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70895.1"
                     /translation="MAKFRVQSHGRLHEWVAEEKGHFRAEGLDYDFVVKPIATWSAHV
                     QVVETAPPEIQRGAFESIEGGRGCDLSTACHWTVNMAASAGHGKMWGGCYSVSPSGIF
                     VPPDSPIKKPEDLAHIPVTVGYHSGSHFSTLQYLEEFLKRDEIKLNFAGLLLDRLALL
                     VDRKVAAASLFGAPFYVAEQLGFRKIIDTTFMIGHLIAPEADQSDVERFFKALRRSQQ
                     EIDMAPEVYKHYFLNELPGRYHSAVDVRMFGPGERLVFESYPKEVFERTRKWIDSWNL
                     FSDEQAGSRGYETSIL"
     gene            1404..2876
                     /locus_tag="VMZ02_02850"
     CDS             1404..2876
                     /locus_tag="VMZ02_02850"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR70896.1"
                     /translation="MAKMLISSELVDSESKETTTIKNPATGEVVDNVPKGTINDIRRA
                     IDAAKGALKKWSSMAPSKRGAILLAAGHTILQREKELATLLTKEQGKPMRESVLEIRR
                     FVHTLDHYGGMAKTMRTSAVMLDTGRHGLVMRKPLGVCGSIVPWNFPVSLMGNKLGPA
                     LLAGNCVVVKPAGTTPLTDLATCEIIDKAIQDAGGPKGVLHCVTGPGSVVGEELLVNP
                     TVRKIGFTGATDTGRRVMQSAAADFKHVTLELGGSDPMIVCDDADIDRAVSAASVGRF
                     FNCGQACLAVKRLYLFDSIADDFISKLAEKAKKISVGNGLKPGVLMGPLHTKDQRQEV
                     EEQVEDAVKRGGRLVFGGKRPTGEDFDKGFYLQPTIVADVDPASRMIQEEVFGPALPI
                     VRVKTLDQAIEHANGSIFGLGSSIWTRDINRAMYGAENIEAGYTWVNSAQIIYDELPF
                     GGVKQSGLGKEHGDEAIEHYTDSKSVVIATDVGGEIIGGE"
     gene            2954..4498
                     /locus_tag="VMZ02_02855"
     CDS             2954..4498
                     /locus_tag="VMZ02_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HUR70897.1"
                     /translation="MNVDLPEIYNAATTFVDENIKQGRGGKVAIYYQDQKITYQQVFD
                     NVNRTGNALKSLGVEIEHRVLVILPDSPEFAYAFFGAIKIGAVAVPTNPWMFAKDYEY
                     LINDSRARAIVVHESTLPEIEKIWDDTPFLKHVLVVGTPKGKALSFESLISSASNQLD
                     AAKTLKDDVCFWGYTSGSTGNPKGAVHLQHDMITISDLFVAPVLQMRDTDLCFSASKM
                     FFSYGLGNSLYFPFRFGASTVLWPDKPEPEKILQVIDKYKPTFFFSVPTLFARLLRID
                     KKYDLSSLRICLSSGEPLPPALFHHWKERTGLELLDVVGSTEATHDFLANRPGRAKAG
                     SSGEVTPAFEAKIVDDEGREVPHGEIGNLMVKGDATAPFYWNKHEQTKRMMQGEWLKT
                     GDTYYRDADGYFWYCGRSDDMMKVGGLWVSPIEIENTLMEHQAVLESGVVSDEDADGL
                     LKPKAYVLLKNEYKASDQLKVELQNHVKSKLQPYKYPRWVEFVDDLPKTVTGKIQRFR
                     LRANKN"
     gene            4554..5264
                     /locus_tag="VMZ02_02860"
     CDS             4554..5264
                     /locus_tag="VMZ02_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR70898.1"
                     /translation="MVANIKELKENLAYSCNILANEGHWDNILGHVSVRVPGQNKILM
                     KPHSFGFEEIRPQHIIVVDIETGKKTDGKYERHSEVFIHTEIMKARKDINCVVHSHPP
                     YATAFGALRQPLRPISHEGSIFYDGLPIFDYTTQLIRTPELGVEVAKSLGSCRGVLMK
                     NHGSTVVGPTIEVATLYAIFLEKAARIQLLATASGEPSWSSDEEAVLKYEQIYTPHRL
                     ESMWEYFVRRAKKFRKSN"
     gene            complement(5346..6332)
                     /locus_tag="VMZ02_02865"
     CDS             complement(5346..6332)
                     /locus_tag="VMZ02_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70899.1"
                     /translation="MHRNVIKLAMTVWALTLSFPAGAQIPVRINWTAVTGAQSGMFMA
                     QQEGIFKKNGLDAELLHISSSSRGIQAILAGEIAFSYMDGVNQVQANLKGANIAFVAG
                     ATNRQVFSLMAKPEIKRISDLRGKKIGITRIGSSTHTSALFALNSAGLKQNDYQILPL
                     VEVPNILTALIAGQVDAGVVSPPTNSRARKAGLNELMNLAKEGPEFVSVAIGTSRSYI
                     KANEDTVRRVVRSYAEGVQLFKTNKGAAQRMMQKYLRVKEQDILDDAYSQFREYLEYP
                     PYVTRKAMESVIAEISVSDAAAKNSKPEDFMDMRFVSELEKQGFFKGSGSKQ"
     gene            6436..7401
                     /locus_tag="VMZ02_02870"
     CDS             6436..7401
                     /locus_tag="VMZ02_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70900.1"
                     /translation="MSKKVHLTLAVGDYESIRALKEGAVKADGIELTVITDMNSDMRH
                     WRMLRNREFDCAELSMSNYLMAKYTGLPFIAIPVFLHRRFRHGFVFLNANKGILKPSD
                     LIGRKVGLRNFQATANLWIRGVLEHEYDLPHRSLHWFKQDDEEVDWTPPADLSIQRIA
                     PGESVVRMLVDGDIDALIHPELIQPILDKDPRVTRLFPNYRALEMDYFKRTGIFPIMH
                     TTAIKQEIVDRYPWIPINLYQALEASKKVAYKRMENPRIVPLAWFRSFLEEQEEMLGP
                     DPWVYGLGDNNRKALETLMQYSQEQGLIGRRLGLDELFINTEAHS"
     gene            7621..7986
                     /locus_tag="VMZ02_02875"
     CDS             7621..7986
                     /locus_tag="VMZ02_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70901.1"
                     /translation="MFGKGVFQQEDNLLREIIRSVDKKIDYSAREGEGSKFTVHLRLK
                     NHEGDVMLDLEDLKAARQDTSRRHQIRQKIKARCDHLDSSRYGADILGLQSARILKTA
                     PKLEPMAQRGGFARTGARR"
     gene            8044..>8838
                     /locus_tag="VMZ02_02880"
     CDS             8044..>8838
                     /locus_tag="VMZ02_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HUR70902.1"
                     /translation="MAKAPMIRQALAKYGQLIMPGIYDALSAKIAARAGFEVTFITGY
                     SLSATVLGEPDFGLLTQTEVVNAAQRICSVIDIPVIVDADTGYGNAINVIRTVQDLIR
                     AGAGGMFLEDQVWPKRCGHMRGKQVISLTEQLNKLRAAVEAKGNEDFFIVARTDSRQA
                     LGLDEAIKRGIAFKDAGADAVFIEAPESKDEMREIAKQVKGHLVANMLERGVTPLMGP
                     EELKELGFDLIVWPLAPLYSGAKSLTDVYTTLRKEGSTLSILDRLMP"
BASE COUNT         2138 a         2368 c         2302 g         2030 t
ORIGIN      
        1 tacaggcggc gctttttgcg tcgcatcggg agcatacagc gcgcctttta ccgaactggg
       61 gacgccggta aaacgaatat cgcgggttgg tctgtgctaa cgcagacgct cctgccagga
      121 agagcatcgc cgtaaaaatc aaagtactca gcgttctgat ctttgtcatc attttttggc
      181 tctctaccat gcgtgcgatc ggccattctt catcgcgagt tcgctagagg atggacgtct
      241 cataacccct gctcccggcc tgttcgtctg aaaaaagatt ccacgaatcg atccacttgc
      301 gcgtccgctc gaaaacttct ttcggataac tttcaaagac caaccgctcg cccggcccaa
      361 acatgcgcac gtcgacagcg ctgtggtagc gcccgggaag ctcgttcaaa aaatagtgct
      421 tgtagacttc cggcgccata tcgatttcct gctggctgcg ccgcagcgct ttgaaaaaac
      481 gttcaacgtc gctctggtcc gcctccggcg caatcaagtg gccgatcatg aacgtcgtgt
      541 caatgatctt gcgaaacccg agttgttcgg cgacgtagaa cggcgccccg aacagactcg
      601 ccgccgcaac cttgcgatcc accaagagcg ccagccggtc cagcagcaaa ccagcaaaat
      661 ttagcttgat ctcatcgcgc ttgaggaact cttcaagata ctgcagcgtc gaaaaatggc
      721 tgccggagtg ataaccgact gtaacgggta tgtgcgccag gtcctcaggc tttttaatag
      781 gcgaatccgg cggcacgaaa atccccgagg gcgacaccga gtagcaaccg ccccacatct
      841 tgccgtgtcc ggcggacgcc gccatgttga ccgtccagtg acacgccgtg cttaaatcgc
      901 aaccacgacc gccttcgatt gattcgaagg cgcctcgctg gatttccggc ggcgccgttt
      961 cgacgacctg cacatgcgcc gaccacgtgg cgatcggttt taccacgaaa tcgtaatcca
     1021 atccctcagc gcgaaagtgg cctttttctt ctgcgaccca ttcgtgcaat cgcccatgcg
     1081 actgaacgcg gaattttgcc atggccaggc ctcccttgtg attctgtcag atcaaactgt
     1141 caattataat cattgcgctt cgacacaaaa taaaaaattg gctgcccctt cgctgcacgg
     1201 catcggcggc tccctggcag gcgtgcaata actaacacag ccaaagccct cggcgctagt
     1261 gttaactccg gagccgccgc ccttttccgc aaccgcgtga tggcggttca atttgaacat
     1321 ttatcgccgc gtttgaattc ttgacttccc ctaatgaaca tgatttttct ctaagttctt
     1381 atagaagttt tgggaggacc cgtatggcaa aaatgctgat ctccagtgag ttggtggatt
     1441 ccgagagcaa ggaaaccacc acaatcaaaa atccagcgac tggtgaagtc gtcgataatg
     1501 ttcccaaagg aaccattaac gatatccgga gagccattga cgccgctaaa ggcgctttga
     1561 aaaaatggtc aagcatggcg ccgtcgaaac gcggcgcgat tttactcgcg gccggacata
     1621 cgattctgca aagagaaaaa gaactcgcga cattgctgac taaagagcaa ggcaagccta
     1681 tgcgcgaatc cgtcctggaa atccgccgct ttgttcatac tctggatcac tacggcggca
     1741 tggcgaaaac tatgcgtacc agcgcggtca tgcttgatac tggccgccat ggcttggtga
     1801 tgcgcaaacc actaggcgtt tgcggttcta tcgtgccatg gaactttccc gtttcgttaa
     1861 tgggtaacaa gctcggccca gcgctgctcg cgggtaactg cgttgtcgtc aaacccgccg
     1921 gcaccacgcc actcacggat ttagcgacct gcgagatcat cgacaaggcg attcaagacg
     1981 ccggcggacc taaaggggtt ttgcactgcg tcacgggtcc gggcagcgtg gtcggtgaag
     2041 aattgctggt caaccccaca gttaggaaga tcggcttcac gggtgccacc gacacgggcc
     2101 ggcgagtgat gcaatccgcc gccgcggatt tcaagcatgt taccttggag ttgggcggca
     2161 gcgatccgat gatcgtctgc gacgacgccg atatcgatcg cgccgtcagt gcggcttccg
     2221 ttggtcgctt ctttaactgc ggccaagcct gcttggccgt caagcggctt tatctattcg
     2281 acagcatcgc cgacgatttc atctcaaaac tcgccgaaaa ggcgaagaag atcagcgtcg
     2341 gcaatggtct gaagcccggt gttttgatgg gcccactgca cactaaggac caacgccaag
     2401 aggttgaaga gcaagttgaa gacgccgtta agcgcggtgg cagactagtt ttcggcggca
     2461 aacggcctac cggcgaggat ttcgataaag gtttttattt acagccgact attgttgccg
     2521 atgtcgatcc ggcatcgcgc atgattcaag aggaggtctt cggtccggcc ttgccaatcg
     2581 ttcgtgtgaa aacactggat caggctatcg aacacgctaa tggctcgatc ttcggtctcg
     2641 gctcgtcgat ctggactcgc gatatcaatc gcgccatgta cggggctgaa aatattgaag
     2701 caggttacac atgggttaat tcagcgcaaa tcatttacga tgaactgccc ttcggcggag
     2761 tgaaacaaag cggcttgggc aaagagcacg gcgacgaagc gattgagcac tacaccgatt
     2821 ctaaatcggt ggtcatcgcg actgatgtcg gcggcgaaat tattggcgga gagtagggtc
     2881 ccattccatt gcagacgaca tattccaggt ttcgcatttg gagtatcgaa tctgagtgct
     2941 ggaatctgca cgcatgaatg tagacttgcc agaaatctac aacgccgcta cgaccttcgt
     3001 tgatgaaaac atcaagcagg gccgtggcgg caaagtcgca atctactacc aagatcaaaa
     3061 aatcacctac cagcaagtct tcgacaacgt caatcgcaca ggcaacgctc taaagagtct
     3121 cggcgtcgag atcgagcatc gcgttctggt gattttgccg gactcacccg agttcgcata
     3181 cgcttttttt ggcgcgatca aaatcggcgc cgttgccgtg ccgaccaatc cctggatgtt
     3241 tgcgaaagac tatgagtatt tgatcaacga cagccgcgcc cgcgctattg tcgtgcatga
     3301 atcaaccttg cccgaaattg aaaagatctg ggacgatacg ccgttcttga agcacgtcct
     3361 cgttgtcggc acgcccaagg gcaaggcgct gtcctttgag agtttaataa gcagcgcgtc
     3421 aaaccaactc gacgcggcta agacattaaa agacgatgtc tgtttctggg gctatacctc
     3481 gggcagtacg ggcaatccca aaggcgccgt gcatttgcag catgacatga tcacgatcag
     3541 cgatctgttc gtcgcccccg tgctgcagat gagagacacc gatctgtgct tttcggcgtc
     3601 aaaaatgttt ttctcttacg gcctgggcaa ctcgctctat tttcccttcc gctttggcgc
     3661 ttcaaccgtg ctttggccgg acaagccgga accggaaaag attctccagg tcatcgataa
     3721 atacaaaccg acattctttt tttccgtgcc gacgctgttc gcccgtttgc tgcgaatcga
     3781 caagaaatat gatctcagtt cgctacgcat ttgcctgtcc tccggcgaac cactgccgcc
     3841 ggcgctgttt catcattgga aagagcgaac cgggttggaa ctgctcgacg ttgtcggctc
     3901 caccgaagct acccatgatt tcttggccaa ccggccgggg agggcaaagg ccggcagcag
     3961 cggcgaagtg acccccgcct ttgaagcgaa gatcgtcgat gacgagggcc gcgaagttcc
     4021 gcacggcgaa atcggcaatt tgatggttaa aggagacgcg acagcgccgt tttattggaa
     4081 caagcatgag cagaccaagc gcatgatgca gggcgagtgg ctcaaaactg gagatactta
     4141 ttatcgtgat gccgatggct atttttggta ctgcggccgt tcggacgata tgatgaaggt
     4201 cggcggctta tgggtttcac cgatcgaaat tgaaaatact ctgatggaac atcaggcggt
     4261 gctcgaatcc ggggtggtga gtgacgaaga tgccgacggg ttactcaagc ccaaagccta
     4321 tgttctactg aagaacgaat acaaggccag cgatcagtta aaagttgaac tgcaaaatca
     4381 cgtcaagtca aaattacagc cttacaaata tccgcgttgg gttgagttcg tcgacgactt
     4441 acccaagacc gtaactggaa agattcaaag gttccgcctg cgggctaaca aaaactgaaa
     4501 tcgatctgcg atcctccatt atccgtagtc gacgttctcg acaaggagac accatggttg
     4561 ccaacatcaa agaactcaaa gaaaatctag cttactcgtg caacatcctc gccaatgaag
     4621 gacactggga taatatcctc ggccacgtgt cagttagagt tcccgggcaa aataagatcc
     4681 tcatgaagcc ccatagcttt ggcttcgaag aaatccgtcc ccagcacatt atcgttgtcg
     4741 atattgagac gggcaaaaag accgacggca agtacgaacg ccactccgag gtctttattc
     4801 acacagaaat catgaaggcg cgcaaggata tcaattgcgt ggtgcattcg catccgccct
     4861 acgctacagc gttcggtgcg ttgcgccaac cgctgcgtcc gatcagtcac gaaggcagta
     4921 ttttttacga cggcttgcca attttcgatt acaccacaca gttaatccgc acacccgaat
     4981 tgggcgtcga agtcgccaaa agcttgggca gttgccgcgg cgttttaatg aagaatcacg
     5041 gctcgaccgt cgtcggaccc accattgaag tggctacgct ttatgcgatc tttttggaaa
     5101 aggcggcgcg cattcagctg ctcgccactg cttccggtga gccgtcgtgg agcagcgatg
     5161 aggaagccgt gctcaaatat gagcagatct acacaccgca caggttggag tctatgtggg
     5221 aatacttcgt tcgtagggca aagaagtttc gcaaatcgaa ctagctcggc tcgtagttta
     5281 tttgtcttga agcttcagca ggagaggctt acgcagtctg aacactagag aaattacgat
     5341 tgtccctact gcttgctgcc gctaccttta aaaaaaccct gcttctcgag ctccgagacg
     5401 aagcgcatat ccataaaatc ttcgggctta gaattcttcg ccgccgcgtc gctgactgaa
     5461 atctcagcaa tgacactttc cattgccttg cgtgtgacat agggcgggta ctctaagtat
     5521 tcacgaaatt gactataggc atcgtccaga atgtcttgct ccttcacccg cagatatttc
     5581 tgcatcattc gctgtgccgc ccccttgttt gttttgaaca actgcacgcc ttcggcatag
     5641 gaccgcacga cccggcgaac cgtgtcttcg ttggccttga tatagcttcg gctggtacca
     5701 atagccaccg agacaaactc tggcccttct ttggctaaat tcatcagttc attcaagccg
     5761 gccttacgcg cacggctatt ggttggcggt gaaaccactc ccgcatcaac ctggccagcg
     5821 atcaaagccg tcaggatatt aggcacttct accaggggca gaatctgata atcgttctgt
     5881 ttcaatccag cagaattaag cgcaaatagc gctgaggtgt gtgtcgacga accaatccgg
     5941 gtaatgccga tttttttccc gcgtaaatcg ctaatccgtt taatttctgg tttggccatc
     6001 agcgaaaaaa cttggcgatt ggtagcgccg gcgacgaagg caatattcgc gcctttcagg
     6061 ttcgcctgaa cttggttaac cccgtccatg taggaaaagg cgatctcgcc ggcgaggatc
     6121 gcttgaatcc cgcgcgaact cgaagagata tgcagcagct cggcgtccaa gccattcttt
     6181 ttaaatatcc cttcctgttg cgccatgaac atgccgcttt gcgcaccggt gacggcggtc
     6241 caattgattc gcacgggtat ctgcgcgccc gccggaaacg ataacgtcaa ggcccaaaca
     6301 gtcatggcaa gttttattac gttccggtgc atatacgttc cctctcaaag acctgaaata
     6361 gccgcattaa cgtaacaatt ctacctgtta attcaactgt ttggcatgtg gggtccgaca
     6421 tgctagatac ggcccatgag caaaaaagtc cacctcactt tggcagttgg cgactatgaa
     6481 agtatccgtg cgctcaaaga aggtgctgta aaagccgacg gcattgagct aaccgtgatc
     6541 accgacatga attcggatat gcgccactgg cgtatgctgc gcaatcgcga attcgattgc
     6601 gcagagttgt ctatgtcgaa ttatttgatg gccaaataca ccggtctgcc attcattgcg
     6661 attccggtat ttctccatcg acgttttcgt catggttttg tgtttttgaa cgctaacaaa
     6721 ggtatcctta aaccgagcga tctgatcggc cgcaaagtcg gtctgcgtaa tttccaagca
     6781 acggcgaacc tgtggattcg cggcgtattg gagcacgagt acgatctgcc gcatcgaagc
     6841 ttgcattggt tcaagcaaga cgacgaagag gttgactgga cgccgccggc ggatttgagc
     6901 atccaacgaa tcgcgcccgg tgagagtgtt gtccgcatgc tggtcgacgg cgatatcgac
     6961 gctttaatcc accctgagtt aattcaaccg attctcgaca aggacccgcg agttacgcgt
     7021 ctgtttccca actatcgcgc gctcgaaatg gattatttca agcgcaccgg gatttttccg
     7081 atcatgcata cgactgcgat caagcaggag attgtcgata ggtatccctg gataccgatc
     7141 aatttgtatc aagcgctgga agcatcgaag aaggttgcct ataaacgcat ggagaatcca
     7201 cggatcgtgc cgctcgcctg gttccgctcc tttctcgaag aacaggaaga gatgctcggt
     7261 cccgatccct gggtttacgg cttgggtgac aacaatcgca aggcgctcga aacgttgatg
     7321 cagtattccc aagaacaagg tctaatcggt cgcaggttgg gactcgacga actgtttatt
     7381 aatacggaag cccattcgta atcggagacc gccgatgctc gcgctcccat gattttcgct
     7441 gacactctcg ccgccgctgt ggcgcattca cgcaatgatt gtcgctttaa tgaagctctt
     7501 ctgaatactt aagagtccca gtgcgatacc tttacgattg aacttggagc gctattggcg
     7561 taaattttcg cattacgatt gcgtcaggat tgcctcatac ggaatcggcg gggagggaat
     7621 atgttcggca aaggcgtatt tcaacaagaa gataatcttc tgcgcgagat catccgcagc
     7681 gttgacaaaa aaatcgatta ctccgcgcgg gaaggcgaag gcagcaaatt caccgttcac
     7741 ctgagactga agaaccacga aggcgacgtc atgctcgatc tcgaggatct caaggctgcc
     7801 cggcaggata cgtctcgtcg gcatcaaatc cgccaaaaaa taaaggcacg ctgcgatcac
     7861 ttggattctt cccgctatgg cgccgacatc cttggcctgc aatcggcgcg aattttgaaa
     7921 accgcgccga agcttgaacc aatggcacaa cgcggcggtt tcgcgcgcac cggcgctagg
     7981 cgctaagcgt tacaacttca ctgactgacc tttctcctta ggtaatcgcg ggttctacag
     8041 catatggcaa aagctccaat gattcgccaa gctctagcca agtatgggca gttgatcatg
     8101 cccgggatct atgatgctct aagcgcaaag attgctgccc gggctggttt tgaggtgact
     8161 tttataaccg gttacagctt atcggcgaca gtgctgggcg aacctgactt tggcctatta
     8221 acccaaaccg aagtggtcaa cgccgcccag cggatctgct ccgtaattga cataccggtg
     8281 atcgtcgatg ccgataccgg ctacggcaat gcaatcaatg taatccgcac cgtacaagac
     8341 ctgatccggg ccggcgccgg cggaatgttc ttggaagatc aagtttggcc gaaacgatgc
     8401 ggccacatgc gaggtaagca agtgatttcc ttaacggaac agctaaacaa gctgcgagcc
     8461 gcggttgagg cgaaaggaaa tgaagatttt ttcatagttg cgcgcacgga ctcacgccag
     8521 gcgctcggac tagatgaagc gattaagcga ggtatcgcct tcaaagacgc tggcgccgac
     8581 gcggtattca tcgaagctcc ggagtccaaa gacgagatgc gcgagatcgc aaaacaggtc
     8641 aaaggacacc tagtggctaa catgttggag cgcggcgtga cccctttgat gggacctgag
     8701 gagttgaaag agctaggttt cgatttgatc gtctggccgc tggcgccgct ctactcgggg
     8761 gcgaaatcgc tgaccgatgt ttacacgact ctgcgcaagg aaggatcgac gctgtcgatt
     8821 ctagaccgct taatgcct
//
LOCUS       DATLCH010000097         4766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3144027, whole genome shotgun sequence.
ACCESSION   DATLCH010000097 DATLCH010000000
VERSION     DATLCH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3144027"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VMZ02_02885"
     CDS             complement(<1..860)
                     /locus_tag="VMZ02_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HUR70903.1"
                     /translation="MSVKDVLETAKKNKVEIIDFKFIDVPGLWQHFSIPATDLDADKF
                     EQGLGFDGSSIRGFQSINESDMILIPDPDTAMMDPFTEHPTMSFVCNVLDAITRQPYS
                     RDPRYITQKAEKYLKSTGIADTSYWGPEIEFFIFDSIRFDQNHHEGYYHIDSAEGFWN
                     SGNGQSANLGNKIRYKEGYFPVPPMDQYQDLRSQMVLNLEKCGIKIEVHHHEVATAGQ
                     TEIDMRFDTMTKMADKVLLYKYVVKNTAKQRGKVVTCMPKPLFQDNGSGMHCHQSLWK
                     TGKNIFYDKKG"
     gene            complement(961..2352)
                     /locus_tag="VMZ02_02890"
     CDS             complement(961..2352)
                     /locus_tag="VMZ02_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HUR70904.1"
                     /translation="MKQAIRTRSSLFFLAVLLVMLMGFSAQAQEPPSGSAPAVTPSAP
                     AAAAAPNTPPAAAKIDKGDNAWMLTSSALVLMMTIPGLFLFYGGLVRSKNILGTIMHS
                     FILVGVVTVQWVLWGYSVAFGPDVGGFFGSLAWVGLNGVGVEPNADYAATIPHMTFMV
                     YQMMFAIITPALITGAFAERAKFSTFLVFMILWTTIVYDPLAHWVWGVKGWLRELGAL
                     DFAGGTVVHISSGASALVAAIMFGRRIGYGQEAMPPHNLPYSIIGAGLLWVGWFGFNA
                     GSALAADGLATSAFVATHVATAAAALAWMAMDWLRGKPTMLGAASGAVAGLVAITPGA
                     GFVSPMSALIIGAVGGILCSLACSLKPKLGYDDSLDVVGVHGVGGTWGALATGLFASK
                     TINPAGNNGLFYGNPAQLWIQFESVIVTIVFAMIVTAIILSILKATMGLRVADEEERM
                     GLDLTQHNERAYE"
     gene            complement(2587..3705)
                     /locus_tag="VMZ02_02895"
     CDS             complement(2587..3705)
                     /locus_tag="VMZ02_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HUR70905.1"
                     /translation="MKISAIDTYALILPVKEIYGGAAGYLDDCRTLIIRVETDNGIEG
                     WGEATQGRPGNTYETLETMQIMVRRYFAPALIGMDLEETGAVIDKLQGVRYGHPITKA
                     GLEIALYDALGKFYNLPVYRLLGGAYSRQIELVGGLGMDLSAETIGAKAEELKHEGYR
                     RFKLKIGHKELQKDIDRVRAVREAVGPEALIRVDGNATYSFSEARELLNALSRFHLSD
                     AEQPLARGDLRSLAELRRSVSIPIAAQESVGSPEEALAVLDAGAADLLKIKLTHIAGF
                     RRALEVAAVVGAKGLSVVIGQGSACTSILSAAEIHLHAAMKNAQRGGEMTGFLRLGQQ
                     SFCSDIGVIRATVTLSNTAGLGITVDKHKLIELAIHDL"
     gene            complement(3702..>4766)
                     /locus_tag="VMZ02_02900"
     CDS             complement(3702..>4766)
                     /locus_tag="VMZ02_02900"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUR70906.1"
                     /translation="PGATAFLPAFAVAENETADGKALLTAFVAGCEVMSRIGVAAGNS
                     LERRGFHAPGLTGTFGAAVAAGRLLKLTEKQLVNALGIAGSYSGGLIEFSRCQEGAMI
                     KRLHLGKAAEGGVTAAQLAGRGFAGPESVLEGKFGFCQSFSDSPKLDYLTHRLGEEWE
                     SMNICIKRCACHINAHAPIEALENLRDELGFRPADIRAITIAGIDKLVTHHANYQPTD
                     LMMAQYSIPFCVALSLYFDPTDPASFDEHKLKDKKITALMKKIHLEVDHEIEEKGWDR
                     AARVTVDLADSRRQSKLIIHFKGTPANPLTTDQVEAKARKLTRGIVSERQLDRLVTSL
                     SHLETITDVSQIGDMLRRAS"
BASE COUNT         1076 a         1289 c         1307 g         1094 t
ORIGIN      
        1 ccctttttgt cgtagaagat attcttgccg gtcttccaca gactctgatg gcaatgcatg
       61 cccgagccgt tgtcctggaa cagcggcttg ggcatacagg tgaccacttt acctctttgt
      121 ttagcggtgt tcttaaccac atatttatag agcagcacct tgtcggccat cttggtcatg
      181 gtatcaaagc gcatgtcgat ctcggtctgc ccggcggtgg cgacttcgtg atgatggact
      241 tcgatcttga tgccgcattt ctctaaattg agtaccatct gcgaacgcag gtcctggtac
      301 tggtccatgg gcgggaccgg gaagtaacct tctttgtaac ggatcttgtt gccgagattg
      361 gcgctctggc cgttgccgga gttccaaaat ccttccgcgg agtcgatatg gtagtagcct
      421 tcgtggtggt tctggtcaaa gcgaatgctg tcgaagatga agaactcgat ttcgggaccc
      481 cagtagctgg tgtcggcgat gccggtcgat tttaaatact tctcggcttt ctgagtgata
      541 tagcggggat cgcgggagta gggctggcgg gtgatcgcat cgagtacgtt acagacaaaa
      601 ctcatggtgg ggtgttcggt gaacggatcc atcatggcgg tgtcgggatc ggggatcaga
      661 atcatgtctg attcgttgat cgactgaaag ccgcggatgc tcgatccgtc aaatccaagc
      721 ccctgttcga atttatcggc atcgagatcg gttgccggta tcgagaaatg ctgccataac
      781 ccgggcacgt cgataaactt gaaatcgatt atctctactt tgttcttctt cgccgtctct
      841 aggacgtctt ttacggacat ctagttgctc ctctcatgtg aatcttgact aatttttttg
      901 aaaacgcaca ccagttatca cattctatca gcgagaacca gcacattacc gctagctcat
      961 tcattcgtag gcgcgctcgt tatgctgggt caaatccaag cccatgcgct cttcttcgtc
     1021 ggcgactcgt aatcccatgg ttgcttttag aatcgacaga atgattgcag tgacgatcat
     1081 ggcaaataca atagtgacga taacactctc gaactggatc cacagctgtg ctggattgcc
     1141 atagaagagg ccgttgttac ccgcggggtt gatcgtcttg gaagcgaaga ggccggtggc
     1201 caacgcgccc caagttccgc cgacgccgtg tacaccgacc acatcgaggg aatcgtcata
     1261 accaagtttt ggctttaggc tgcaagcgag cgagcagagg atgccaccga ccgcgccgat
     1321 aatcagggcg gacatcggac tgacgaaacc cgcgcccggc gtgatcgcaa cgagtcctgc
     1381 gaccgcgccg ctggcggcgc caagcatcgt cggtttgccg cgcaaccagt ccatcgccat
     1441 ccatgccagc gctgcggcag cggtggcgac atgggtcgcg acaaaagcag atgttgctaa
     1501 tccgtcggca gcaagcgcgc taccggcgtt gaaaccaaac cagccaaccc agagaagccc
     1561 ggcgccgatg atgctgtagg gcaagttatg cggcggcatc gcctcttgac cgtagccgat
     1621 tcggcggccg aacatgattg ccgccactaa ggctgaagct ccggagctga tgtgcacaac
     1681 cgtgccgccg gcaaagtcta acgctcccag ctcgcgcagc caacctttga ccccccaaac
     1741 ccaatgggcg agaggatcgt aaacgattgt tgtccacaaa atcataaaca ccagaaatgt
     1801 actgaacttg gctcgctcgg cgaacgcgcc ggtgattaac gctggcgtga tgatggcgaa
     1861 catcatctga taaaccataa acgtcatgtg aggaattgtg gcggcataat ccgcgttggg
     1921 ctcgacgcct acaccattca acccgaccca ggcgagcgaa ccgaagaaac cgccaacgtc
     1981 aggtccgaaa gccacggaat aaccccaaag gacccattgc acggtgacga cgccgactag
     2041 aataaaacta tgcatgatcg tgccgagaat atttttgctg cgcacgagtc cgccgtagaa
     2101 gagaaacaga cccggaatcg tcatcatcag cacgagggcg ctagaggtaa gcatccacgc
     2161 gttatcgcct ttatctattt tcgctgcggc gggcggtgtg tttggcgccg ccgcggcggc
     2221 gggagctgat ggggttaccg ctggggccga cccggacgga ggctcttgag cttgcgctga
     2281 aaatcccatc aacatgacca acaataccgc caggaaaaac aacgacgagc gagttctaat
     2341 cgcctgcttc acggcatacc ccctaatttg ctgcaagaat attaaacacc caggataaga
     2401 aaaatcttat ccgggtgacg gagcaaccgt tatgccatta gccgattatt ttatttttct
     2461 ttattttcaa agacttccga tttcaccgct gacaatttcc cgctcccgat gcctattttt
     2521 aaagcatgtg atcgaaaatt cagcagcgtc acagaaacga aataaactaa ggagggggac
     2581 tgaaggttac aaatcgtgaa ttgccagctc gataagctta tgcttatcca cagtgatgcc
     2641 gagaccggca gtgttactca gtgtcacggt agctcgtatt acaccgatgt cactgcaaaa
     2701 cgactgctgg ccgagacgga ggaagccggt catttcgcca ccgcgttgag catttttcat
     2761 agccgcgtgc agatgtatct cagccgccga aaggatggac gtgcaggcgc taccttgacc
     2821 gatgactacg gatagtcctt tggcaccgac tacggcggca acttcgagcg cgcggcgaaa
     2881 gccggcgatg tgagtaagct tgattttcaa caagtcggcg gctccggcat ccaggaccgc
     2941 taatgcttct tcaggagaac caacgctttc ctgggccgcg atcgggatac ttacagaccg
     3001 gcgcagttcg gctagactcc tgagatcccc gcgcgccaaa ggctgctcag catcggatag
     3061 atgaaaacgg ctcaaggcat tcaacaactc ccgggcttcg ctgaacgaat aggttgcgtt
     3121 accgtcgacg cgaatcagcg cttcgggacc gacagcttct cggacggcgc gcacgcgatc
     3181 gatgtctttt tgaagttctt tatggccaat ctttagtttg aaccggcgat agccctcgtg
     3241 ttttaattcc tcagctttcg caccgatagt ttcggcgctc aaatccatgc cgaggccgcc
     3301 gaccagttct atctgacggc tatatgctcc ccctagcagg cgataaacgg gcaagttgta
     3361 gaactttccc aaggcgtcgt agagggcgat ctctaatccc gctttagtaa tcggatgacc
     3421 ataacgcacg ccttgtagtt tatcgatgac cgcaccggtc tcttcgagat ccatgccgat
     3481 tagcgccggc gcaaaataac ggcgcaccat gatttgcatg gtttccaagg tttcgtaggt
     3541 gttgccgggg cggccttggg tcgcttcgcc ccagccctcg atgccattat cggtctcgac
     3601 cctgataatg agggtccggc agtcgtcaag gtagccggcg gcgccaccgt agatttcttt
     3661 taccggtaga atcagcgcat aggtgtcaat cgcagagatc ttcatgacgc gcggcgtaac
     3721 atgtcgccga tttgtgaaac gtccgtgatt gtttccagat ggcttaacga agttaccagg
     3781 cgatccaact gccgctctga aacgatgccg cgcgtgagct tgcgcgcttt ggcctcaact
     3841 tggtccgtgg tgaggggatt ggcgggcgtt cccttaaaat ggatgattag tttcgattgt
     3901 cgccggctat cggcgagatc gacggttacg cgcgcggcac ggtcccagcc tttttcttcg
     3961 atctcgtgat ctacttcgag atgaattttt ttcatcagag cggtgatttt tttatctttg
     4021 agtttgtgct cgtcgaagct cgccggatcg gtgggatcga aatagaggct tagagcgacg
     4081 caaaagggaa tgctgtactg cgccatcatc agatcggtag gttggtagtt ggcgtggtga
     4141 gtgaccaatt tatcgatgcc ggcgatggtg atggcgcgaa tgtcggcggg tcgaaagcca
     4201 agttcgtcgc gcaaattctc cagggcctcg atgggtgcgt gagcattaat atggcaagcg
     4261 cagcgcttga tacaaatgtt catgctttcc cattcttctc ccagcctgtg ggtaagatag
     4321 tcgagtttgg gcgaatcgga aaaactttgg caaaagccga acttgccctc gagcacgctt
     4381 tccggcccgg caaagccgcg gccagccaac tgtgctgcag tgacaccgcc ttcagctgct
     4441 ttgccgagat ggagccgctt gatcatcgcg ccttcctggc agcgcgagaa ctcgatcaat
     4501 ccgcccgaat aggagccggc aatacccaaa gcgttgacca attgtttttc agttaatttc
     4561 aataaacgcc cggcggcgac ggccgcgccg aatgtgccgg ttaaccctgg cgcatggaaa
     4621 ccgcggcgct ccaatgaatt gcctgctgct acgccgatac gcgacatgac ttcgcaacca
     4681 gcaacgaacg cggtgaggag ggccttacca tcggcggttt cgttctccgc taccgcgaag
     4741 gctggcaaaa atgccgtggc accggg
//
LOCUS       DATLCH010000098         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1054638, whole genome shotgun sequence.
ACCESSION   DATLCH010000098 DATLCH010000000
VERSION     DATLCH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054638"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..479
                     /locus_tag="VMZ02_02905"
     CDS             138..479
                     /locus_tag="VMZ02_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70907.1"
                     /translation="MPTLLVVVGAITNGIFNPANSTAMIAMMPKEHRGFASAINHVTF
                     GFGSVFGVALGGLSMSLAFEHYTGVRAATLTTENAAGFVAALNLTFVAAIVLTLVAIV
                     TSALRGAEKPG"
     gene            617..1576
                     /locus_tag="VMZ02_02910"
     CDS             617..1576
                     /locus_tag="VMZ02_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HUR70908.1"
                     /translation="MSEKIVYLNGVFVPENEAKVSVLDSGFNAGDGVYDVTRTFAHQP
                     FRLRDHTARLFRSLRYTRIDCGLSIEQMEQATLAVLERNRPTLAKDEDVAIWQVVSRG
                     MRSSRGNHSLGKATVAIYCISIGFEEFARDYLDGVKLVIPSTRRIPPQCLEPKAKINN
                     KMNHNMAIFEARQVDPHAIPLMLDLDGNISESNAHNFFLVLDGKLCTPLNKNVLDGIT
                     KQVVHELAANLEIEVVEGNFTAYDAYNAEEAFLASTSPTILPVQSINGVRPTQEIPGP
                     MTRRLIQAWNELVGLDIVEQALGHLESTERDRLLKEWRSRSAS"
     gene            1610..>2241
                     /locus_tag="VMZ02_02915"
     CDS             1610..>2241
                     /locus_tag="VMZ02_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70909.1"
                     /translation="MKNIIATAVMVGVLFSLTASISGQTLKTGYISKDLNYLPFFIAQ
                     KKGFHAKEGIAVDLVSIGRSDIQLQALVAGELHFANINADNIIIWNERTGGNLKVAAG
                     SSNAAPYLLIGAKNIKRIEELKGQRIGVNSLSGGATSILVSYLRSKGLLHPRDYSMSV
                     IAGGTPARLSALESGAVAGAVLVLPFSDIAIDKGFTRLGDTTEVISSYQF"
BASE COUNT          505 a          600 c          650 g          486 t
ORIGIN      
        1 tcaccgtggc gctggcgcca ttaagcggct acttggccga ccggctcggc ccccgcattc
       61 ccgccacggt cggagtcgtg ttcatgattg tgtcgctggc cctcggcgga ctgctgcgcg
      121 tcgactagca ttggctgatg ccgacgttgc tggtcgtggt cggtgccatc accaatggca
      181 tattcaatcc cgccaactcg acggcgatga tcgccatgat gccgaaggag caccgtggtt
      241 tcgcctcggc gatcaatcat gtgaccttcg gtttcggcag tgtgttcggc gtggcgctgg
      301 gcggtttgtc gatgtccttg gcgttcgagc attataccgg cgtgagagcg gcgacattga
      361 cgaccgagaa tgccgccgga ttcgtcgccg cgctcaatct cacctttgtc gcagcgattg
      421 ttttgaccct ggtagcgatt gtcacgtcag cgctgcgcgg cgcagagaag ccgggctagg
      481 agacatttcc gatttcagtt tccaaaaaat aatattgcag ccgtggtgga agtacgctgc
      541 gtcaaggaaa ttcggttgta atcgaacttc gatctgagaa taatccggcc agtgcacgaa
      601 ctaaaggagg aggctcatga gcgaaaagat cgtttatctc aatggcgtat tcgtgcccga
      661 gaacgaagcc aaagtatcgg tgctcgactc cgggttcaat gccggcgacg gggtttacga
      721 tgtcacccgc acgtttgcgc atcaaccttt tcgtctgcgc gatcatacgg cgcggctgtt
      781 tcgatcgctg cgctatacgc gtatcgactg tggcctatca atagaacaga tggagcaggc
      841 aaccctcgct gtgctcgaac gtaacaggcc aacgctggcc aaggacgaag atgtggcgat
      901 ctggcaggtg gtgagccgcg gcatgcgctc gtcgcgcggc aatcattctt tgggcaaagc
      961 gacggtggca atttattgta tctccatcgg cttcgaagaa ttcgcgcggg attatctcga
     1021 cggcgtgaag ctggtcattc cgtcgacgcg gcggattcca ccgcagtgtt tggagcccaa
     1081 ggccaagatc aacaataaaa tgaatcacaa catggcgatt ttcgaagcgc gccaggtcga
     1141 tccgcacgcg atcccgctga tgctcgatct cgacgggaac atcagtgaat ccaacgccca
     1201 caactttttt ctcgtcctcg acggcaagct gtgcacgcca ttgaacaaga acgtgctcga
     1261 cggtatcacc aagcaagtcg tgcacgagct ggcggcgaat ttggaaatcg aggtcgtcga
     1321 aggcaatttc acggcctacg atgcttacaa cgccgaagag gcatttcttg ccagcaccag
     1381 cccaacgatt ctgccggtgc agagcatcaa cggggtcaga ccgacccagg agattcccgg
     1441 acccatgacc aggcgcctga tccaggcttg gaatgaactg gttggcttag acattgtcga
     1501 gcaagccttg gggcatttgg aatcgaccga gcgtgatcgt ttgttaaaag agtggcggag
     1561 cagaagcgcg tcgtaagacg cgccaaagtc aggaacagcc gaggacccaa tgaaaaatat
     1621 tatcgccact gcagtgatgg tgggagtttt gttttcgctc acagcgtcga tatcggggca
     1681 aactctcaaa accgggtaca tctcaaagga cctgaactac ctgccatttt ttatcgcgca
     1741 gaaaaaaggt tttcacgcca aagaagggat cgcggtggat ctggtcagca tcggccgtag
     1801 cgacatacag ctccaagccc tggtggcagg cgagcttcac ttcgccaata tcaacgccga
     1861 caatattatc atctggaatg agcgcaccgg cggcaatcta aaagttgccg cgggctcgag
     1921 caatgcggca ccgtatcttc tgatcggtgc caagaacatc aagcgcattg aagagttaaa
     1981 aggtcaacgg atcggcgtca atagcttgag cggcggggcg acgtcaatct tggtcagcta
     2041 tttacgcagt aaaggtctgc tgcacccgcg tgactattcg atgtcggtga tcgccggagg
     2101 cacgccggcg cggttgagcg cacttgaaag cggcgcggtg gccggtgctg tgctggtttt
     2161 gccattctcc gatatcgcaa tcgacaaagg ttttactaga ctgggcgaca ccaccgaggt
     2221 tatttcgagc taccaattca a
//
LOCUS       DATLCH010000099         4853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5527147, whole genome shotgun sequence.
ACCESSION   DATLCH010000099 DATLCH010000000
VERSION     DATLCH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5527147"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VMZ02_02920"
     CDS             complement(<1..606)
                     /locus_tag="VMZ02_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70910.1"
                     /translation="MPRKVTMISGDSHLDFSPERWTHHVPEKWRERAPRRIKLANGDD
                     AFIVENSRPHSPSLQITGTGTAYDKHDARGISYEGPGTGTPAQRLSEQDQDGIDAEIL
                     YTHPSYLASWRGITDNEPYVAMIHAYNEWLIEEYCSFAPDRLVGTGIIPDTNLSDAIA
                     ELKYCAKAGYKSVCLYKFPSGKGYPVVDDDQFWAAAVDLNMP"
     gene            complement(728..1762)
                     /locus_tag="VMZ02_02925"
     CDS             complement(728..1762)
                     /locus_tag="VMZ02_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70911.1"
                     /translation="MPSEQKNRFDHLASLHDKYARLGLRGLWQRDNRSAPEVVPRIWR
                     WSDMKPILEETLSAVRLPEDTDQRVIGLDVPGLSTGSVFVSYQMINPGEKIPSHRHTP
                     AQMRFIVQGSGAYTTSQGEAMFMEPGDLLVQPNWTWHGTTDPGNEPVFWIDIQDRNLV
                     NYLGAFRRDLWPNNDVEPTVHRDNYYAQQTGLLRPSHVSTEDDVLPPIHYKWRDMTNA
                     LDEIAESAGLSPYDGRVFEYRHPSTGGHTLPTLSAQLQLLPPGAKTKEHRHTGMAMYL
                     VVKGEGSTTVNGTTIEWHEHDCFLLPAWQWHAHQNAAQNDRAILFTVSDRPALEALGL
                     YYEEGRAGMG"
     gene            complement(2284..3297)
                     /locus_tag="VMZ02_02930"
     CDS             complement(2284..3297)
                     /locus_tag="VMZ02_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70912.1"
                     /translation="MQLPPIAATTIGSFPRPSWLAQNERSRAVFRLEGAELKQAQDDA
                     TALSIHTQERIGIDLLSDGEQRRTGFINHVLASFDGIDLEHEAVKTIYRRREQPRPVP
                     RVIGKITRRRQAIVEDLKFAKAQTDKPIKMDVPGPMTIVDSTLDEFYKGDEAAMAMDA
                     ATAINQELRDLQAAGCDVLEIDEPAMTRYHEKVFAYGAKALDRCLEGITVPTMVHLCY
                     GYPGGGGRQHEYEYPELLEELMKTRIGGFGVEFARSGYDPAVLSIAKGRVVMFGCVDP
                     SDAPVPSVSSVAERVRSVLKYVEPKNLWLAPDCGLMTITRELANAKAKLLVDVAKEVR
                     RTL"
     gene            complement(3451..4110)
                     /locus_tag="VMZ02_02935"
     CDS             complement(3451..4110)
                     /locus_tag="VMZ02_02935"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HUR70913.1"
                     /translation="MGGIEILHEDRDVIVIDKPAGLLTISTEREKSRTAYFILTDYVR
                     KSMAKSRNRIFIVHRLDRETSGILIFAKNEEAKFRLQSQWQDTTKEYLAVVHGRCEMR
                     EETITTYLAENQAHGVYTTRDARKGKLSHTAYKVLKETKDFSLLEVTLLTGRKHQIRV
                     HLADIGHPVVGDRRYGKEHKAYQRLALHARAISFKQPFTGEQLTFTSKMPVFFNSLIG
                     Q"
     gene            complement(4200..>4853)
                     /locus_tag="VMZ02_02940"
     CDS             complement(4200..>4853)
                     /locus_tag="VMZ02_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70914.1"
                     /translation="DNGDQIVIEVKAWESSPDSTARAINQAHRYKELSKAGAAVVVTS
                     TVLESIASSAIAVTPMGGLIGALARLANILAKKKRPTKAQFIHPAPNKKVFASMPFAQ
                     MYDDTFLVAIEPASLANGAIAERVDHNGTVGPVVPQIKDMIKTSKVIVADLSDSRPNV
                     LHEVGYAEALGKPVIQICSTSTSLLPFNVRNNQTIKYAIGQTAKLKKSLEAEIKKYV"
BASE COUNT          970 a         1307 c         1380 g         1196 t
ORIGIN      
        1 cggcatgttt aagtcgacgg cggcggccca gaactgatcg tcgtcgacaa ctggataacc
       61 tttgccactg ggaaatttat aaaggcagac gctcttgtag ccggctttgg cgcaatattt
      121 aagctccgcg atggcatctg acaaattggt gtcggggatg atacccgtgc cgaccaaacg
      181 gtcgggcgcg aacgagcagt actcttcgat gagccactca ttgtaagcgt ggatcatcgc
      241 cacgtagggc tcgttgtcag tgatgccgcg ccaagatgcc aggtaactcg ggtgcgtgta
      301 caagatttcg gcgtcgatgc cgtcttgatc ctgctcgctc agccgctgtg ccggcgtgcc
      361 ggtgccggga ccttcgtagc tgatgccgcg ggcgtcgtgc ttatcgtacg ccgtgccggt
      421 cccggtgatt tgcaagcttg gtgaatgagg acggctattc tcgacaatga aagcatcgtc
      481 gccgttggca agcttgatcc gccgcggtgc gcgctcgcgc catttctccg gcacatggtg
      541 ggtccagcgc tccggtgaaa agtcaagatg ggaatctcca gagatcatgg taactttgcg
      601 cggcataagc aggctccttg tcttttgctt ttctacttcg atttttagaa tggccatagt
      661 tggagtgatg gagtgttgga atatgggctt gcggagccga caatgactcc attgctccag
      721 tactccatca ccccattcct gcgcgtcctt cttcgtaata caaacccagc gcttcgagcg
      781 ccgggcggtc gctgacggta aagaggatag cgcgatcgtt ctgtgccgca ttctgatgcg
      841 cgtgccattg ccaagcgggc agcagaaaac agtcgtgctc atgccattcg atagtcgtgc
      901 cgtttaccgt cgtcgagcct tcgcctttga cgacaagata catcgccatt ccggtgtgcc
      961 ggtgctcttt ggtttttgct ccgggcggca acaactgcaa ctgcgcgctg agggtcggca
     1021 gcgtgtggcc gccggtgctc ggatggcgat actcgaacac tctgccgtcg tagggactca
     1081 agccggcgct ctcggcaatt tcatcgagcg cgttggtcat gtcgcgccac ttgtaatgaa
     1141 tcggcggaag cacatcgtct tcggtggaga catgtgacgg ccgaagcaag ccggtctgct
     1201 gagcgtagta attgtcccga tgcaccgttg gctcgacatc gttgttgggc cacagatcgc
     1261 ggcgaaatgc gccgaggtaa ttcaccaaat tgcgatcttg aatgtcgatc caaaacaccg
     1321 gctcattgcc aggatcggtg gtgccgtgcc aggtccaatt gggctgcacc agcaaatcgc
     1381 ccggttccat gaacatagct tcgccttgcg aggtcgtgta ggcgccgctg ccttggacga
     1441 tgaagcgcat ctgcgccggc gtatggcgat gagacggaat cttctcaccg ggattgatca
     1501 tttgataaga gacgaatacg gatcccgtcg ataaccctgg cacgtccaga ccgatgacac
     1561 gctgatcagt atcttccggc aaacgcacag cgctcaaggt ctcctcaagt atcggtttca
     1621 tgtcgctcca gcgccaaatg cgcggcacaa cctcgggcgc gctccgattg tcgcgctgcc
     1681 acaggccgcg caatccgagc ctcgcgtatt tatcatgcag cgaggcaaga tgatcgaaac
     1741 ggtttttttg ctcggatggc atggctctga ttctccactc agcttgtgtt aacaatagct
     1801 acgatgaaga tattctccga tcaccctgag gctctcgaag ggtgtcttcg ctgagctaat
     1861 cttgatactt cggcccagtc tctttcgatc agtgcttttt tctttttccg actccaaccc
     1921 ttaatcctct gctcagaggc gaaagcttct tctcgtgttg gaaagtcctc tgagaaaact
     1981 aacttcactg ggagtctcct cgaggtataa cctccggttt cagctgtctg atgttttgcc
     2041 attctttctt caagttgtcc gtatgaccag tgtagtgagc catcggcaca atataaaatg
     2101 taaacccaaa agctcatcaa tgctcaacct atttcacggt gtaatagtca aacaacgcca
     2161 tcggagccct tcgacaaagc tcagggcgac cggagtggag gagactcttt gctaatttct
     2221 cttcgagctg tccggtgcca agccgaagga tcaaagcacc cagctgtcat tactggtaaa
     2281 caactagagc gtccgccgca cttccttggc gacgtcgacc agcaatttcg ccttcgcgtt
     2341 cgctaattcg cgggtgatcg tcatcaagcc gcaatccggc gcgagccaga gatttttcgg
     2401 ctcgacatat tttaataccg aacgcacgcg ttctgcgacg gaggataccg acggtaccgg
     2461 tgcatcactc gggtcgacgc agccaaacat gaccactcgg cctttggcga tgcttagcac
     2521 cgcgggatcg taaccgctgc gggcgaactc gacgccaaag ccgccgattc tcgttttcat
     2581 caactcttcg agcagttcgg gatattcata ttcatgctgc cggccgccgc cgcccggata
     2641 gccgtagcag agatggacca tagtcggcac ggtgatgcct tcaagacagc ggtcgagcgc
     2701 cttggcgccg taggcaaaca ctttttcgtg ataacgcgtc atcgccggtt cgtcgatctc
     2761 cagcacatcg cagccggcgg cttgcaggtc gcgcagctcc tggttgatcg ctgttgcggc
     2821 atccattgcc atggcggctt cgtcgccttt gtaaaactcg tccagcgtac tgtcgacgat
     2881 ggtcataggt ccgggcacat ccatcttgat cggtttgtcg gtttgcgcct tggcgaattt
     2941 gagatcctcg acgatcgcct gtcgtcgccg agtgatcttg ccaatgacgc gcggcaccgg
     3001 ccgcggctgc tcgcgccggc ggtagatcgt cttcaccgcc tcgtgttcga gatcgatgcc
     3061 gtcgaaagaa gcgagcacat gattgataaa tcccgtgcgc cgctgctcgc cgtcggacaa
     3121 cagatcaatg ccgatgcgtt cttgagtgtg aatgctcagc gccgtcgcgt cgtcttgcgc
     3181 ttgtttgagt tccgcccctt cgaggcgaaa gaccgcgcgg ctgcgctcat tttgcgccag
     3241 ccagctgggc cggggaaagc tgccgatggt cgtggcggcg atgggtggaa gctgcatggt
     3301 tcacctcgat aaggaattgt ctttggctat atatgcggca gcgcggaaag acaagttgaa
     3361 atcaatgaag aatggatagt ggagaattga aaactgccgg aacactgatt tatggcagta
     3421 cgactctagc ttggttgcgc taggcggtga tcattgtcct atcaacgaat tgaaaaaaac
     3481 cggcatcttc gaagtaaatg ttagttgctc accagtgaag ggttgcttga aggaaattgc
     3541 gcgggcatga agcgctaggc gttggtaagc tttatgttct ttgccgtaac gcctatcgcc
     3601 gaccaccggg tggccgatat cggcgaggtg cacgcggatc tgatgcttcc gcccggttaa
     3661 gagagtgact tccaaaagcg agaaatcttt ggtctctttc agcaccttgt aggctgtgtg
     3721 agacagtttt cccttgcgtg cgtcgcgcgt cgtgtacact ccatgcgcct ggttctcggc
     3781 cagatacgtg gtgatggttt cctcgcgcat ctcacagcgg ccatggacga cggcaagata
     3841 ctctttcgtg gtatcctgcc actggctctg caaacgaaac ttcgcctctt cgttcttggc
     3901 gaaaatgagt ataccggacg tctcgcgatc gagccggtgg acaatgaaaa tcctgttgcg
     3961 ggatttcgcc atactcttgc gcacatagtc ggtgagaatg aagtaggcgg tgcgcgattt
     4021 ctcgcgctcg gtgctgatgg tcagcaggcc cgcgggtttg tcgatgacga tgacatcgcg
     4081 gtcttcgtgc agaatttcga tgccgcccaa gggatgtttg ccatttgatc gggaagatcg
     4141 ccgcattttc gctgttttag tttatcgaat gtttattcga cgaggaactg tcgaatggtt
     4201 cacacgtatt ttttgatctc tgcttccaac gattttttga gcttcgcggt ttggccgatt
     4261 gcatacttga tcgtctgatt gttgcgaacg ttgaacggca gcagggatgt ggaagtgcta
     4321 caaatttgaa tcaccggttt gcccagcgcc tcggcgtaac cgacttcatg cagcacgttg
     4381 gggcgcgagt ccgataggtc ggcaacgatg accttggagg ttttaatcat gtccttgatt
     4441 tgcggaacga ctggaccgac tgtgccgtta tggtctaccc gctcggcaat ggcgccgttg
     4501 gcaagcgatg ccggttcaat ggcgaccagg aaagtgtcgt cgtacatttg cgcgaagggc
     4561 atcgacgcga ataccttttt attcggtgcc ggatggataa attgcgcctt tgtcggtctt
     4621 ttctttttgg caagtatgtt tgcgaggcgc gcgagggcgc cgatgagtcc acccatggga
     4681 gtgacggcaa tcgccgacga tgcgatggat tcgagcacgg tggaggtcac gaccaccgcg
     4741 gcgccggctt tcgacagctc tttatatctg tgtgcttggt tgatcgcacg agcggtcgag
     4801 tccggactgc tctcccaggc tttgacctcg atgacgattt gatccccgtt atc
//
LOCUS       DATLCH010000100         4610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4775973, whole genome shotgun sequence.
ACCESSION   DATLCH010000100 DATLCH010000000
VERSION     DATLCH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4775973"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VMZ02_02945"
     CDS             <1..599
                     /locus_tag="VMZ02_02945"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HUR70915.1"
                     /translation="ISGITLALFVIFATFDSKAQPAPAANPTNISGKVVSLKGPELIV
                     AIPSGEGTVAISEKTVVRAEVPIKFSEITSGMYVGATATKQADGNFLASQINIFSEDQ
                     RGTGEGHRPLGAATMTNANVERVEDVTVREVKGRMLNLKYKEGEINVIVPPDIPINKR
                     VVGDRSLIKTGSEVSVTADKQADGSLTARQITVRAPKP"
     gene            727..1176
                     /locus_tag="VMZ02_02950"
     CDS             727..1176
                     /locus_tag="VMZ02_02950"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HUR70916.1"
                     /translation="MKKLFRQFGVISTIFSLALLVVVGCSTTNSSSQEGMKVTLSGNQ
                     EVPPVKTSATGNATVTIKPDKSVSGTISTTGLDATAAHIHEGEAGKNGPVIVPFTKAA
                     DGTWSTAPGAKLTDAQYASYLAGNLYVNVHTAANKGGEIRAQLKPSK"
     gene            1225..1698
                     /locus_tag="VMZ02_02955"
     CDS             1225..1698
                     /locus_tag="VMZ02_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HUR70917.1"
                     /translation="MVPGQILMFATVLLLSGLASSIPVFAQEDVIEKRKAIMRANNDA
                     VSKSIKKAVEEKDYATIELKAKDIMAGMDAIVNLVPPGSTSEKSRAHPDIWVKTDDFK
                     NKALAARKAAEALSKAAAAKNDEEINVKVKELGNNQNGACGECHKIYRTDFRKGG"
     gene            1824..2828
                     /locus_tag="VMZ02_02960"
     CDS             1824..2828
                     /locus_tag="VMZ02_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70918.1"
                     /translation="MAVKKVSFIIIGFFLQLVLIPLAAAQMTRLNVGYSATSADQLPA
                     WVAKDSGIFAKNGLDAQVIFFTGGTTAVLALVSGDVPIAQISGPGVVNSVLAGSDVVF
                     IAAGTVSLNYVLMGKPGVKSVDQMKGGTIAISRFGSATDTIARFALRRIGLTPGKDVT
                     LIQVGSGPERLSALLTGRVTAAVINPPSSFQAEKKGLAVLADVAKMGLVFQHTGAVTT
                     KKFIREQPDIVRRYVRAHVEAVARMWNDKEASIKALARFMGSNIDRETLERTRADVMH
                     ESLYPKKQYPSVEGIKTVIEDIAERDPRAKNVKPEQFIDATFIRELDQSGFIDGLYKK
                     "
     gene            2842..3102
                     /locus_tag="VMZ02_02965"
     CDS             2842..3102
                     /locus_tag="VMZ02_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70919.1"
                     /translation="MLSKHRGDLATAELQGWAQEVANRVDPAVQTEVLFEEDSNTFIL
                     RLAKDARVLVFRLSASQVYTDGREPECERTLIRKIKDLWNLL"
     gene            complement(3185..4162)
                     /gene="hemB"
                     /locus_tag="VMZ02_02970"
     CDS             complement(3185..4162)
                     /gene="hemB"
                     /locus_tag="VMZ02_02970"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HUR70920.1"
                     /translation="MDFPRYRPRRMRRNDRLRAMMRETALAPANLIYPLFVGPGKNKV
                     QRVSSMPGVAQLSVDRALGECEEAQALGIPAVILFGLPEHKDSVGAEAYAESGIVQQA
                     IREIKAKLPELLVITDVCLCEYTDHGHCGVIKNGDVDNDSTLELLVKESLSHARAGAD
                     IVAPSDMMDGRVGAIRKALDDNGFPQIAIMAYAAKYASGFYGPFREAAESTPQFGDRR
                     SYQMDPANSDEALREVELDIREGADIVMVKPAMAYLDVIYRVKQKFGYPVAAYNVSGE
                     YSMIKAAGQNGWIDEERIMMEVLYAIRRAGADMVLTYFAKEAARLLARG"
     gene            complement(4177..>4610)
                     /locus_tag="VMZ02_02975"
     CDS             complement(4177..>4610)
                     /locus_tag="VMZ02_02975"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HUR70921.1"
                     /translation="AEGILESLEPSVLVGKHVLIPRAAKARNILPETLRQWGATVDVV
                     EAYCTVAPAFDGATIRQRLQQGDIDMITFTSSSTVSNFVQLCGNPSLAALVGATAIAS
                     IGPITAQTIEDLGGHASVMAREFTIDGLIVAMIEHFDGGKN"
BASE COUNT         1127 a         1270 c         1250 g          963 t
ORIGIN      
        1 taatatctgg aataacgctc gcgttattcg ttattttcgc aacctttgat tccaaggcac
       61 aacccgctcc cgcggccaac ccaacgaata tttccggcaa agtcgtatct ttgaaaggtc
      121 cggaattaat cgtcgcaatt cctagcggtg agggaacagt ggctatcagc gaaaaaaccg
      181 tcgtccgggc cgaagtcccg atcaagttct ccgaaattac ttcaggcatg tatgttggcg
      241 cgaccgcgac caaacaagcg gacggaaact ttctggcctc tcaaatcaat attttctcag
      301 aagaccaacg cggaaccggt gaagggcacc gtccgttggg tgctgccacg atgaccaatg
      361 cgaacgtcga gcgcgttgaa gacgtcactg tgcgggaagt caaaggacgt atgttgaacc
      421 tcaaatataa agagggcgag ataaacgtga ttgtgccgcc ggacattccg ataaataaac
      481 gagtggtagg agatcgcagc ctgatcaaaa ccggctccga agtgtcagta accgcggata
      541 agcaggccga tggttcgctg accgcacgac agatcaccgt gcgtgcgccc aagccctaat
      601 taacacgacg caaaggagga tgacgactat tccgccagag cgggacggat cttctcgcca
      661 cggaatactg aatgacttaa gcagaagaaa aggcaatata tcgaacgcag gaaaagcgag
      721 gaaaacatga aaaaactatt tcggcaattt ggagtaattt cgacgatttt ttctttggcg
      781 ctgctcgttg tcgtcggctg ctcgacgaca aatagctcca gccaagaagg catgaaagta
      841 acattaagcg gcaaccaaga ggtgccgccg gtgaagacat cggcaactgg aaacgccaca
      901 gttacgatta aaccggacaa gtccgtcagc ggcactattt cgacgaccgg actcgatgcc
      961 actgcggcgc atattcatga gggcgaagct ggtaaaaacg gccccgtaat tgtgcctttt
     1021 accaaggccg ccgatggtac ctggtccacc gctccgggag cgaagctcac cgatgctcaa
     1081 tacgcgagct acttagcggg caatctttac gtgaatgtac ataccgcggc aaacaagggc
     1141 ggagagatcc gcgcgcaact aaaaccgagc aaataatttc gtaggtcaaa cggtaaaatt
     1201 tcaatcgaga gaggaaaata caacatggta ccaggtcaaa ttttaatgtt cgctacagtt
     1261 ctgctgctat cgggcctggc gagttcgatt ccagtctttg cgcaggaaga tgtgatagag
     1321 aaacgcaaag cgattatgcg agcgaacaac gacgcagtct cgaaatcgat caaaaaagcc
     1381 gtggaggaaa aagattacgc caccatcgag ttgaaggcca aggacattat ggccggaatg
     1441 gatgccatcg taaatcttgt cccgcctgga agtacgagcg aaaagtctcg ggcccatccc
     1501 gatatttggg tcaagaccga tgacttcaaa aacaaggcgc ttgcggcgcg caaggcggcg
     1561 gaggccttga gcaaagccgc ggcggccaag aacgacgagg aaattaacgt caaagtcaaa
     1621 gaattaggta ataatcagaa tggcgcctgc ggcgagtgtc acaaaattta tcgcaccgat
     1681 ttccgcaagg gcgggtgaag agcgccaatt actggcttca ttatcttctc gaagaaagcc
     1741 tctgcctcgc ggcaggggct ttcttgtttc ggctcgaagc gttcgctact attctgtcct
     1801 attctgactc gaggtgattt tacatggcag tgaaaaaggt aagtttcata atcataggct
     1861 ttttcttgca gctggttctc ataccactgg ccgcggcaca gatgacacgg ctcaatgtcg
     1921 gctacagcgc caccagcgcc gatcagctgc cggcttgggt agccaaagat agcggcatct
     1981 tcgccaagaa tggtttggac gcgcaggtaa tctttttcac gggcggtacc acggcggtgc
     2041 tcgcgctggt atccggcgat gtcccgatcg cacaaatttc tggtccaggc gtcgttaaca
     2101 gtgtactcgc cgggtccgac gtggtgttca ttgctgccgg caccgtctcg ctcaactacg
     2161 ttcttatggg caaaccggga gtgaagagcg tcgatcagat gaaaggcggc accattgcca
     2221 tcagccgctt cgggtccgcc actgacacca tcgctcgctt cgccctgcgt cgcatcggac
     2281 tcaccccggg gaaggatgtc acgctcatcc aagtcggcag cggaccggaa cggctgagcg
     2341 ctttgctcac cggtagagtg acggcagctg tgatcaaccc gccctcaagt ttccaggccg
     2401 aaaaaaaggg cttggcagta ctcgccgacg tcgccaaaat gggcttggtg ttccaacata
     2461 ccggcgcggt gacgaccaag aaatttattc gcgagcaacc cgacatcgtg cgtcgctacg
     2521 tgcgcgccca tgtcgaggcc gtggcgcgta tgtggaacga caaagaagct tcaatcaaag
     2581 cgctcgctcg ttttatgggc agcaacatcg accgcgagac tttagagagg acccgcgctg
     2641 acgtgatgca cgaatcgctt tatcccaaga agcagtaccc cagcgtcgaa gggatcaaga
     2701 cggtcatcga ggatatcgct gaacgcgatc cgcgggcgaa aaacgtcaag ccggaacagt
     2761 tcatcgacgc cacctttatc cgcgaactcg atcaaagcgg cttcatcgac ggcctttaca
     2821 aaaaatagcc cggcagatgg aatgctaagc aagcatcgcg gtgacctggc aacggctgaa
     2881 ttacagggct gggctcaaga ggtagctaac cgcgtcgatc ctgccgtcca gaccgaggtt
     2941 cttttcgaag aagactccaa taccttcatc ctacgcttgg ccaaagacgc tcgagtgctg
     3001 gtctttcgac tctccgcctc gcaggtctac actgacggtc gcgagccgga atgcgagaga
     3061 actctcattc gtaaaatcaa agatttgtgg aatttattgt agcggaattc ggctatccgc
     3121 acgaagcagc gaaactaagg aaacaatatt tgaagttcgt gctgttcgtg tctttcgggt
     3181 tgtcttatcc gcgcgcaagt aagcgcgccg cttctttggc aaaataagtc agaaccatgt
     3241 cggcaccggc gcggcggatc gcgtagagca cttccatcat gattctttcc tcgtcgatcc
     3301 agccgttctg gcccgccgct ttgatcatcg agtattcacc gctgacattg taagccgcca
     3361 ctgggtaacc gaacttctgt ttgactcgat agattacatc cagataggcc attgccggtt
     3421 tcaccatgac gatgtcggcc ccttcgcgga tatccagttc gacttcgcgt agcgcttcgt
     3481 cggaattggc cgggtccatc tgataggaac ggcggtcgcc gaactgcggc gtcgactcgg
     3541 ccgcctcgcg aaacggcccg taaaaaccgg acgcatactt ggccgcgtag gccatgatcg
     3601 cgatttgcgg gaagccgttg tcatcgagtg ctttgcggat cgcgccgacc cgaccgtcca
     3661 tcatatccga cggcgcaaca atgtctgcgc ccgcgcgggc gtgagacaag gattctttga
     3721 ccaatagctc caacgtacta tcgttgtcga cgtcgccgtt tttgattaca ccgcaatggc
     3781 cgtggtcggt gtattcacat aaacaaacat ccgtgatgac gagcagctcc ggcagcttgg
     3841 ccttgatctc gcgaatcgct tgttgaacga tgccgctttc tgcatacgcc tcggcgccaa
     3901 cggaatcctt atgctcgggc agaccgaata aaatgaccgc gggaatccca agagcctggg
     3961 cttcctcgca ttccccaagc gcgcggtcca ccgacagttg cgccacaccc ggcatcgacg
     4021 acacccgctg gactttgttt ttacccggcc caacgaacaa cggataaatc aaattggccg
     4081 gcgccagcgc ggtttcgcgc atcatcgccc gaagtctgtc gttgcgccgc attcggcgcg
     4141 gccgatatcg tgggaagtcc atgcggtgct ccggggttaa ttcttgccgc cgtcaaagtg
     4201 ctcgatcatc gccacgatca agccatctat agtgaactct cgcgccatga cggacgcgtg
     4261 gccgcctaag tcttcaatgg tctgggccgt gatcggcccg atactggcga tagcggtggc
     4321 gccaaccagc gccgcgagcg aagggttgcc acacagctga acgaaattac tcaccgtact
     4381 cgaactggta aaggtaatca tatcgatgtc gccctgttgt aaacgctgac ggattgtcgc
     4441 gccgtcgaac gctggcgcga cggtgcaata ggcttctacc acatccactg tcgcacccca
     4501 ttggcgcaac gtctcgggca aaatattgcg cgctttggcc gcgcgtggaa ttaaaacgtg
     4561 cttgccgacc agcactgatg gctccaggga ttcgagaatg ccttcggcct
//
LOCUS       DATLCH010000101         4853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5531171, whole genome shotgun sequence.
ACCESSION   DATLCH010000101 DATLCH010000000
VERSION     DATLCH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5531171"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="VMZ02_02980"
     CDS             <1..1608
                     /locus_tag="VMZ02_02980"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HUR70922.1"
                     /translation="DYFPLALKVIRKIEAIVRQEMDRAGAQELLMPISSPAELWQESG
                     RWDIYGKELIRFKDRHDRDFCLGPTHEEIVTDLVRRSVRSYRDLPFNLYQLQTKFRDE
                     VRPRFGLMRGREFIMKDAYSFHADVEDCRREYDNMYETYKRIFTRCGLSFRPVEADTG
                     AIGGSHSHEFQVLAESGEDAIVGCDSCEYAANVEKAEVRGKLPAGRDILEESPQLARV
                     STPGKKTVPELTNFLHLSADKVIKTIVYSTETGELIAVLVRGDHEINELKLRAVLGCR
                     EVNLADETAVVAATGVPTGYLGPIGMKLRMVADLSVHGMRGAAAGANEANAHFISVDQ
                     ERDFTPSAFADLRLAVSGDPCPRCDSGKLQTHRGIEVGQVFYLGTKYSEKMGATYLDA
                     EGREQAIEMGCYGIGISRMVAAAVEQNHDANGIIWPLSIAPFQVLVLPINYKEEKLRG
                     AAEKLYRDLQCVDVEVMLDDRDERAGVKFKDADLIGIPLRVTIGAKGLDKGFIELRTR
                     SDGKTDEVPLGEAVKTIQTIIAQAVRG"
     gene            1612..2367
                     /gene="pyrF"
                     /locus_tag="VMZ02_02985"
     CDS             1612..2367
                     /gene="pyrF"
                     /locus_tag="VMZ02_02985"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HUR70923.1"
                     /translation="MSSKLELVRKHGSMRERLIVALDFDTLEQATDLVRQLSGEVGMF
                     KIGKQLFTHAGPQAVRQIQELGGEIFLDLKFHDIPNTVAKAAIEATRLGVKMFNVHAS
                     GSLEMMRMTVKEVKRVCRQQKLRKPIMLAVTVLTSLNQDDLERVGVERKVSDQVVRLA
                     LLTKEAGMDGVVASPHEVGDIREACGRRFVIVTPGIRPADKNRNDQQRVMTPQDAVCG
                     GVDYIVVGRPIIEARDPVSAARAIVADMQLGGR"
     gene            complement(2427..3221)
                     /gene="thiD"
                     /locus_tag="VMZ02_02990"
     CDS             complement(2427..3221)
                     /gene="thiD"
                     /locus_tag="VMZ02_02990"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HUR70924.1"
                     /translation="MTIRKVLTIAGSDSGAGAGIQADLKTFAAHGVYGTSAITAITAQ
                     NTTGVTAVLALNPNLVGAQIDAILADIGTHAMKTGMLANAGIIDLVARKIAEHRLKNV
                     VVDPVIVATSGDLLLQKSAVAVLRSKLIPLATIVTPNIPEAEVITGMKLRTAGEIREA
                     AQRIIKMGAKTVVIKGGHLNGPAIDLFFDGKIFTALKTKRIRTINTHGTGCTLSAAIA
                     ANLAKKQNIASAVANAKRYITDALRHSYRIGSGHGPVHHFYRYWKS"
     gene            3622..4515
                     /locus_tag="VMZ02_02995"
     CDS             3622..4515
                     /locus_tag="VMZ02_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70925.1"
                     /translation="MSTSNSSTLKTLLGDYPNTKALKSGELRSANVSFEFADVKVPNT
                     AFKDVVRHLKYDVAELAIVTFLQAKAHGKPLVLLPAVVVGALPHPLLVYNAEHRRILP
                     ADLKGKRVGIRAYSVTTAAWVRGVLQNDYGVDLDEIEWVTFEDAHVAEYSDPPTAVRA
                     PAGKSIVQMLSDHEIDAGVVGGPDLKDTRFQPVIENPHDAAQHWCNKHKALPINHMLV
                     VKESLANTNPFALQEIFRLLRESKLAMPRAETALDTIQFGLANIRRSLELIISYSTQQ
                     GLIPEQISVDDLFDEVTGNLI"
     gene            4573..>4853
                     /locus_tag="VMZ02_03000"
     CDS             4573..>4853
                     /locus_tag="VMZ02_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70926.1"
                     /translation="MKLIGLISPNSSEETLADVYKIFPPGVRIEARTLSVGQYTDDEF
                     HRAELTFQGLVRDLVKQPLDFLMITGELFLSYKGPGSDREILQLVKQITT"
BASE COUNT         1099 a         1288 c         1393 g         1073 t
ORIGIN      
        1 gattatttcc ccttggcgtt aaaggtgatc cgtaagatcg aagccatcgt acgtcaagag
       61 atggaccgcg ccggcgccca agaactgctg atgccgattt cctctcccgc agaactgtgg
      121 caagaaagcg gacgatggga catttacggc aaggaactga tccgtttcaa ggaccgccat
      181 gaccgggatt tctgtctcgg tcccacccat gaggagatag tcacggattt ggtacggcgt
      241 tcggtgcgct cttaccgcga cctaccgttc aatctgtatc agctccagac caagtttcgc
      301 gacgaagtgc ggccgcgttt cggcttaatg cgcgggcgcg agttcatcat gaaagacgcc
      361 tatagttttc atgccgatgt cgaggattgc cggcgcgaat acgacaacat gtatgagacc
      421 tataagcgca ttttcacccg ctgcggtctg agtttccggc cggtggaagc ggataccggt
      481 gcgatcggcg gtagccattc gcatgagttc caagtgctcg ccgagtctgg cgaagacgcc
      541 atcgtcggtt gcgacagctg cgaatacgct gccaatgtgg agaaggctga agtcaggggc
      601 aagttgccgg cagggcggga cattctcgag gaatcgccgc aactcgcaag ggtctcgaca
      661 cccggaaaga aaaccgttcc cgagctcacg aattttttgc atctcagcgc cgataaggtc
      721 atcaagacga ttgtttacag tactgaaacc ggcgagctga tcgcggtcct ggtgcgtggc
      781 gatcatgaaa tcaacgagct gaaactgcgc gcggtgctcg gctgccgcga agtaaatctg
      841 gctgacgaaa ccgcggtagt ggcggctacc ggtgtgccaa ccggctattt ggggccgatc
      901 ggaatgaagc tgcgtatggt tgctgatttg tcggtgcacg gcatgcgtgg cgcggccgcc
      961 ggcgctaacg aagccaacgc tcatttcatc agtgtcgatc aggagcgcga tttcacgccg
     1021 agtgcatttg ccgatttgcg tctggccgtc agcggcgatc cctgtccgcg ctgcgattcc
     1081 ggcaagctac agacccaccg cggcatcgaa gtcggccaag ttttttatct gggcactaaa
     1141 tatagcgaga agatgggcgc cacttatcta gacgccgagg ggcgtgagca agccatcgag
     1201 atgggctgtt acggcatcgg catcagccgc atggtcgctg cggctgtgga acagaaccac
     1261 gatgccaacg gcattatctg gccattgtcc atcgcgccgt ttcaggtgtt ggtgctgccg
     1321 atcaattaca aagaagagaa gttgcgcggg gccgccgaga aactctatcg cgacttgcag
     1381 tgtgtcgacg tcgaagtcat gctcgacgac cgtgacgagc gagctggcgt aaagttcaaa
     1441 gatgccgatc tgatcggcat tccattacgt gtaaccatcg gcgccaaggg attggacaaa
     1501 gggtttatcg agttgcgcac ccggagcgat ggcaagactg acgaagtgcc gctcggcgag
     1561 gcggtgaaaa cgatccagac gatcatcgct caggccgttc ggggctaggt catgagttca
     1621 aagctcgagc tggtacgtaa acatggttcc atgcgcgagc gattgatcgt cgcccttgac
     1681 ttcgacactt tggaacaagc cactgatcta gtgcgccaac tttccggcga agtcggcatg
     1741 ttcaaaatcg gcaagcagtt gttcacccac gctggaccgc aggccgttcg gcagatccaa
     1801 gagctgggcg gcgaaatctt tcttgacctg aagtttcacg acattcccaa tacagtagcc
     1861 aaagcggcca tcgaagcgac ccgattaggc gtgaagatgt tcaacgtgca cgcttcgggc
     1921 agcctcgaaa tgatgcgcat gaccgtcaaa gaagtgaagc gggtgtgccg gcaacaaaag
     1981 ctgcgcaaac cgatcatgct tgcggtcacc gtgctgacga gcttgaacca ggacgactta
     2041 gagcgcgtcg gcgtcgagcg caaggtttct gatcaggttg tgcgcttggc cctgctaacc
     2101 aaggaagccg gtatggatgg tgtggtggct tcgccccatg aagtcggcga tatccgcgag
     2161 gcgtgcggcc ggcgctttgt tattgtcacg ccaggaattc gccccgcgga taaaaatcgt
     2221 aacgaccagc aacgcgtcat gacaccgcaa gacgccgtgt gcggcggcgt cgactacatt
     2281 gttgtcggtc ggccgatcat cgaagcaaga gatccagtgt ccgcggcgcg cgccatcgtc
     2341 gcggatatgc agcttggcgg caggtaacct cgtcgcctcg tttgctcgat tccggatgct
     2401 gattcgcgcc agcgcagatc tgccgcctac gacttccaat atcgatagaa atgatgcacc
     2461 gggccgtgcc ccgagccgat gcgatagctg tggcgcaacg catcggtaat gtagcgctta
     2521 gcgtttgcaa ctgcgcttgc aatattctgc ttctttgcga gattcgccgc gatagcggcc
     2581 gagagagtgc agcccgtgcc gtgggtgtta atagttcgaa tccgcttcgt tttgagcgcg
     2641 gtaaagatct tgccgtcgaa aaatagatcg atcgcgggcc cgttcagatg accgcctttt
     2701 atgacgacag ttttggcgcc cattttgatg atccgctgcg ccgcttctct aatttctccg
     2761 gctgttcgaa gcttcatacc ggtgataact tctgcctcgg gaatgttcgg tgttacgatg
     2821 gtggctaacg gaatcagttt gctgcgcaac actgcgaccg cacttttttg aagcagcaaa
     2881 tcgccggaag ttgcgactat gaccggatcg acgacgacgt ttttcaagcg gtgttcggcg
     2941 attttccgcg ccaccaagtc gatgatgcca gcattggcga gcatgccggt tttcatcgca
     3001 tgagtgccga tgtcggccag aatcgcgtcg atctgcgcgc cgaccaggtt tggattaagc
     3061 gccaaaaccg cggtaacgcc tgtggtattc tgcgccgtaa tcgccgtgat agctgacgtg
     3121 ccgtagacgc catgggcggc gaaagttttc agatcggctt ggatgccggc gccggcacct
     3181 gagtcggagc cggctatggt gagaactttg cggatggtca taattattgc tctctcgcgg
     3241 aatcactcac ttataagact gtagcggtta tgtagcaatc gaagtaaacc actgtcttcc
     3301 cgatggcggc actcgctggt aatggcgagt cgcttgatcc ggttcaaatg agcgccggca
     3361 aaaaaatacc tccacgatgt ggaacgccga cttctaaacc gtcaattcaa caggctgcgt
     3421 cgattgctcg ggtgaaatga caatgctacg taaggaaaca aaagctaatt ctagtgagtg
     3481 cacgggactg ttcccaaatt gacagttgtg tcgaggtcgc ggttttctcg agctcggacc
     3541 gatgcgaacg gataagagct gacgtataac aactcaaact ctgcggacgt cgttaacgtt
     3601 cgttaaaagg aaccgactgg catgagtact tcgaatagtt cgaccctcaa aacgcttctc
     3661 ggcgattacc ccaacactaa agcactcaag agcggcgaac ttcgttcagc gaacgtcagc
     3721 ttcgagtttg ccgatgtgaa agtgccaaat accgccttca aggatgtcgt gcgccacttg
     3781 aagtatgacg ttgccgagct ggcgatcgtt actttcttgc aggcaaaggc gcatggtaaa
     3841 ccgctggtgt tgttgcccgc cgtggtcgtc ggcgctttgc cccatccatt gctggtttac
     3901 aatgccgagc atcgccggat cttgcccgcc gatctcaaag gcaaacgcgt cggcattcgc
     3961 gcttactcgg taaccacggc tgcttgggtt cgcggcgttc tacagaacga ttacggagtc
     4021 gatctggacg aaattgagtg ggtaactttc gaagacgccc acgtcgccga atattccgat
     4081 ccgccgactg cagttcgcgc acccgccggc aagtcgatcg tgcaaatgct cagcgatcac
     4141 gagatagacg ccggcgtggt cggcggtcct gatctcaaag atacgcggtt ccagccggtc
     4201 atcgaaaatc cccatgacgc cgcccaacac tggtgcaaca aacacaaggc gttgccgatc
     4261 aaccacatgc tggtggtcaa ggagtcctta gcaaatacca acccattcgc gttgcaggaa
     4321 atttttcgac tgcttcgtga aagtaagcta gcgatgccgc gggcagaaac tgcgctcgat
     4381 acgattcaat tcgggttggc aaacattcgg cgcagtctcg agctgatcat tagctattcg
     4441 acccagcaag gactcattcc agagcagatt tcggtggacg atttgtttga cgaggtgaca
     4501 ggaaatctca tttgaacatg gccaatgtca aaaataatca tgccaattac gagcaaaccc
     4561 aggagaacga gaatgaaact aatcggactg atttcaccga attccagtga agagacattg
     4621 gcggatgttt acaagatctt cccgccgggc gttcgcatcg aagcgcggac attgagtgtc
     4681 ggccagtaca ccgacgatga atttcaccgt gccgagttaa ctttccaagg gctggtgcgc
     4741 gatctagtca agcagccttt ggattttttg atgatcactg gagaattgtt tctttcgtac
     4801 aaaggcccgg gctctgatcg ggagatcctt caactggtta agcagattac gac
//
LOCUS       DATLCH010000102         2771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10597047, whole genome shotgun sequence.
ACCESSION   DATLCH010000102 DATLCH010000000
VERSION     DATLCH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10597047"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VMZ02_03005"
     CDS             <1..1076
                     /locus_tag="VMZ02_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70927.1"
                     /translation="KISTDSIVGAGATYRKWVESEGIPLVESFFVENIATVPLEPWKR
                     TGGLGVRLCLEGTGETNDAYICEIPPGKSLAPEKHMYEELIYVISGRGAATVWNEGEP
                     KRTFEWQEGSLFSPPLNSWHEIFNGNGSEPARFLAVTSAPCMMNLIHNSDFMFNCPYV
                     FHDRYQSQEDYFGNPGTWYSGRTWRTNFVADVKNLKLLEWRERGGGGSQVRLELSENT
                     LCGHISQFAVGNYKKAHFHGPGAHVIILAGEGYSLLWPRGQEIKRFDWQPGSLVVPPG
                     GWFHQHFNSGSVPVRYLALRWGSQKYHEMWGEGRGKADVDVKLGGNQIEYEDEDPIVR
                     KMFSQACAKAGVTNLMEAYYPAA"
     gene            complement(1092..1694)
                     /locus_tag="VMZ02_03010"
     CDS             complement(1092..1694)
                     /locus_tag="VMZ02_03010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR70928.1"
                     /translation="MADISDPWDHHDWASRDYVLEWADRQDDSEPHRDQAFGVIAATM
                     PFDRDAPIKILDIGSGYGALTFYLLSQFPNAQAVCHDGSAEMLALGRERLAKFKDRVK
                     FIQSDLSEPGWSKKLEGPFDAVVSSIAIHNVREHATIRSIYGESFELLNPGGWFLNLD
                     RMRPSVNEQLDWLRQAGYQQVQQFWDGGKRAIVGGFRQSD"
     gene            complement(1768..>2771)
                     /locus_tag="VMZ02_03015"
     CDS             complement(1768..>2771)
                     /locus_tag="VMZ02_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70929.1"
                     /translation="MRKRILGLMIFGFMIGAAHGQDAKLVDAAKKEGGKVVVYSSMES
                     SISDGIAAGFKKKTGLEMEYWRGSSTKVLDRALNEHRAGKPLFDVAITIADPMFLMQR
                     EGVFAKYESPAAADYPKALIDANLGPNYRTLLIGIVYNTNSIKPADAPKSFEDLVKPQ
                     FKGKVIMPDPTQHTTTTQWVASLPKLMGKDRADKFIRDLAATKPILAESLLPPVERAV
                     SGEVPIAITLLHYTYVFRQQGAPLDYVRMPKLLGDGNYVGLGNKAPHPNAGKAFIDYF
                     LDDECMNLMSKLGEFVNRKGIVPPLEGVNKIEFVPMDRLDAKTYAEKKKEYQKLFLQ"
BASE COUNT          605 a          771 c          755 g          640 t
ORIGIN      
        1 caaagatttc caccgattcg attgtcggcg cgggcgcgac ttatcgaaaa tgggtggaga
       61 gcgaaggcat tccgctggta gagagctttt tcgtcgaaaa tatagcaacc gtgcctctgg
      121 agccctggaa acgcaccggc ggcttgggcg tgcgcctatg cttggaaggc accggcgaaa
      181 ccaacgatgc ctacatatgc gagattccac ccggaaaatc cctggcgccg gaaaagcaca
      241 tgtacgagga gttgatttac gttatcagcg gccgcggcgc ggcaacggta tggaacgaag
      301 gcgagcccaa gcggacgttc gagtggcagg aaggctcgct gttctcgccg ccgctcaatt
      361 cctggcatga aatttttaac ggaaatggca gcgagcctgc ccgttttctc gccgtcacca
      421 gcgcgccctg catgatgaat ctgattcaca atagcgattt tatgttcaat tgtccctacg
      481 tctttcacga tcgctatcag tctcaggagg attacttcgg caatcccggc acctggtatt
      541 ccggccgcac ctggcgcacc aacttcgtcg ccgatgtcaa gaatttgaaa ctcttagaat
      601 ggagagagcg cggcggcggc ggctcgcagg tgcgtttgga attgtccgag aacacgcttt
      661 gcggccatat ctcgcagttc gccgtcggca attacaaaaa agctcacttt cacggtcccg
      721 gcgcccatgt gatcatcctt gccggcgaag gctactcgct gctgtggccg cgcggtcagg
      781 agatcaaacg cttcgattgg cagcccggca gcctcgtcgt cccgcccggc gggtggttcc
      841 atcagcattt taattccggt tccgtcccgg tgcgctacct agcgctgcgc tggggtagcc
      901 aaaagtatca cgagatgtgg ggcgaaggcc gcggcaaagc cgacgtcgat gttaaactcg
      961 gcggcaacca gatcgaatat gaagacgaag acccgatcgt cagaaaaatg ttttcgcagg
     1021 catgcgccaa agccggcgtg accaatttga tggaagcgta ttacccagca gcttgaaatt
     1081 cgtcgcctgt ttcagtcaga ttggcggaat cccccaacga tggcgcgctt gccgccgtcc
     1141 caaaactgct gaacttgctg gtagccggct tggcgcagcc aatcgagctg ttcattcacc
     1201 gatggccgca ttcggtcgag attcaaaaac cagccgccag gattcagcaa ctcaaaactt
     1261 tcgccgtaga tcgaacgaat ggttgcgtgc tcacgcacat tgtggatcgc gatcgacgac
     1321 accaccgcat cgaaaggtcc ttcgagtttt ttgctccacc ccggttcgct caagtcgctc
     1381 tgaatgaact tgacgcggtc tttaaatttg gccaagcgct cccgaccgag cgcgagcatc
     1441 tccgccgaac cgtcgtgaca taccgcttga gcgttgggga actggcttag aagatagaac
     1501 gtcaatgcgc cataacccga gcctatatcg agaatcttga ttggcgcatc cctgtcgaat
     1561 ggcatggtcg cggcgataac gccaaaggct tggtcgcgat gaggttcgct gtcatcttga
     1621 cggtcggccc attcgagcac gtagtcgcgc gatgcccaat cgtgatgatc ccacggatcg
     1681 gaaatatcag ccatttactt acctccttga gaaccaaaac ggaatgattt aaatgcttgc
     1741 attgttcccg catgtcaact cttcagtcta ctgcaaaaat agtttttgat actctttctt
     1801 tttctcggcg taggttttgg catcgagccg gtccatcggc acgaactcaa ttttgttcac
     1861 gccttcgagc ggcggcacga tgcctttacg attgacgaac tcgccgagct tggacatgag
     1921 attcatgcat tcgtcatcga gaaaataatc gatgaacgct ttgcccgcgt tgggatgcgg
     1981 tgctttgttg cccaagccga cgtaattgcc gtcgccaagc aacttgggca tgcgcacata
     2041 atccaaaggc gcgccctgct gacggaacac gtaggtgtaa tgcaagagcg tgatcgcgat
     2101 cgggacttcg ccgctgaccg cgcgttcgac cggcggcagt agcgactcgg caagaatcgg
     2161 tttcgtcgca gccaagtcgc ggataaattt gtcggcgcga tctttgccca ttaatttcgg
     2221 cagactcgcg acccactggg tggttgtcgt atgttgcgtc ggatcgggca tgatcacttt
     2281 gcctttgaac tgtggcttca cgagatcttc aaatgacttc ggcgcgtcag cgggcttgat
     2341 cgagtttgtg ttgtaaacga tgccgatgag aagcgtgcgg taattcggcc cgagattggc
     2401 atcgatcaac gctttgggat agtccgccgc cgcgggcgat tcatatttgg cgaacacacc
     2461 ctccctttgc atcaagaaca tcggatcggc gattgtgatc gcgacatcga agagcggctt
     2521 gccggcgcgg tgctcgttga gggcgcggtc gagaaccttg gttgacgatc ctctccaata
     2581 ttccatctcc aggccagtct tcttcttaaa gcccgcggca ataccatccg agatgctcga
     2641 ctccatcgag ctgtagacaa ccactttgcc gccttctttt tttgccgcgt cgacgagctt
     2701 ggcatcttga ccatgagcgg caccaatcat aaacccgaaa atcattagac ctagtattct
     2761 tttacgcatg g
//
LOCUS       DATLCH010000103         5919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9262673, whole genome shotgun sequence.
ACCESSION   DATLCH010000103 DATLCH010000000
VERSION     DATLCH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9262673"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VMZ02_03020"
     CDS             complement(<1..336)
                     /locus_tag="VMZ02_03020"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HUR70930.1"
                     /translation="MASSRGATLLGPDKLEVREYPMPDIPPDGGLLRVEMGGVCGTDV
                     KYLHGKLKVDYPIILGHEILGRIDKLGKNAAAIHHVKEGDRVILKGALGCGRCADCRR
                     NNQRFCRQRT"
     gene            complement(428..1441)
                     /locus_tag="VMZ02_03025"
     CDS             complement(428..1441)
                     /locus_tag="VMZ02_03025"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70931.1"
                     /translation="MGTFTSKERIMTIVRRMVRLSIIAWMCGQTNTIGAANINVVWTS
                     EVGQFAPLWVTKEAKLFEKYGNNVQLIFIQGASSAAAALSSGDAHVGMFSPQVVISTP
                     QLDLVMFGRLGNTMDNRVYARKGIKNIKEVKRVAISRFGSNADFAARFLLQREGLRPD
                     IDVALLQVGNQANRIAAVETNNADAAMLTPPMTLQARKLGMTLLLDASKLNIPYSSLF
                     FVARRSYLGKMRPELVNFTKAMIEGVALYKSNKEFAMKVLSKYMKVQDREVLEENFKE
                     YDFPLKPYPSREYFELPIQEVGKKDPKVLKENPDRYTDLSLVKELESSGFIDKVSQEH
                     RVK"
     gene            1511..2491
                     /locus_tag="VMZ02_03030"
     CDS             1511..2491
                     /locus_tag="VMZ02_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR70932.1"
                     /translation="MIQSSLAVLFLFWLVSFASAQSPTRVMSGYAATSGPHAVLWIAR
                     DAGLFEKNGLRTDVAYIRSGSTMAQTLVSGDIQMSQMGGPAMLAAGVAGMDVTFVAVA
                     LNTTPIVLMGTVSKVEELKGKAVGVTRYGSNTDISARFAIRKAGLQPEKDVALVQLED
                     YAGIMGGIASGRIAAGALADPFTDAAKKLGYKEVADVAAMGLEFPFVGIAAKKSYIKD
                     NVDTVQRFVRAYTEAIAMYKNNRDLAMKITGKYTGIKDPAILASTVDFYAPKLQRVPF
                     ATVGGLRFVLDQVAVRDPRAKNYTPETFMDMRFVKQLEDSGFIKALYPKG"
     gene            complement(2498..3691)
                     /locus_tag="VMZ02_03035"
     CDS             complement(2498..3691)
                     /locus_tag="VMZ02_03035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HUR70933.1"
                     /translation="MASASQINRIKAGFVGVGLGLAFLMSLNWWNIYAHHVPKCSTDN
                     CVADFVTFYAAAQLMWDDPQSLYDLDRQQLYQNRIAPVERVLPFVYPPITAAFLAPLA
                     WFEFSTAFLIMTLVNLLLIGQSLRLLIRGLHLSSDQSHWLLLFTLCNFAVHGVIFYGQ
                     TSALVLFFLTAHVLAQRQAERPVAGVCAGLLCIKPQYLAIPHLIMLVRRRWREFLVGA
                     AVASVLIIGLFLWIGFEASTQYFRLARRMVSADSDWWNQWRGMHNLRVLVIYGLPTAW
                     HALTWWLLSALIVGILIWINWPEAKHSDDHATRWIVNCLGLMIVLPHLFTHDLTLLII
                     PCALLLSLIKPQVRPVMGIGLAALAILPAINYLIPTIMASALLMLFIASLFLARNKIV
                     ISQNC"
     gene            complement(3746..4210)
                     /locus_tag="VMZ02_03040"
     CDS             complement(3746..4210)
                     /locus_tag="VMZ02_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70934.1"
                     /translation="MHLIAFAMWIGAMVFFLVVMGSAVHDMEPSIAIRTMNRGRIGLE
                     AISWIGIAVLLITGIANLALGNPSAVTTGGAYMALLGGKLFVFMAMTVHHCLQVFKYA
                     PSIARQTAALNPSAAVWPEALLSDWRRWFVLLKLNAGLGPLAVLLGMALSRS"
     gene            4397..5062
                     /gene="coxB"
                     /locus_tag="VMZ02_03045"
     CDS             4397..5062
                     /gene="coxB"
                     /locus_tag="VMZ02_03045"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HUR70935.1"
                     /translation="MLRWLPEDVSTYGHEIDSLFYLIYYITAVTFVLVTVLMVWFLIK
                     YREQPGRRAIYNHGNTTLEIIWTVIPAAILIVLSFMSVNTWAKIKRNIPASDFEIQVT
                     AKQFNWDVVYPGADGKFGTEDDVKFDNDLHVPVNKVVRVHLMSSDVIHSFFIPNLRFK
                     QDAVPGRTILTWFEAKKAGRYELPCAELCGFGHSGMKGWLNVHTPEDYQKWAAENLAP
                     KPQ"
     gene            5108..>5919
                     /locus_tag="VMZ02_03050"
     CDS             5108..>5919
                     /locus_tag="VMZ02_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HUR70936.1"
                     /translation="MSESAVAHGAHGHHAKPSFVSHYLFSLDHKMIARQFLFVGLLML
                     LIGGILAMLVRWQLAWPETPVPGLGFISEPYIYPGPNGGGIIPPQTYNALFTMHATIM
                     IFFVVMPIMVGTFGNFLIPLMIGAGDMAFPKLNMLSFWFGAVSGVIMLASFFVPGGPA
                     AGGWTGYATLSARAQYTGVEWGQHLWIIALMVNGISSLMGAINYITTIVNMRAPGMGY
                     FRMPLTVWSLFVTAILLLLALPVLTAALAMLLFDRMAGTSFFLPEGGGEPLL"
BASE COUNT         1306 a         1558 c         1645 g         1410 t
ORIGIN      
        1 ggtgcgttgg cggcagaaac gttgattatt gcgccggcag tcagcgcagc ggccgcagcc
       61 gagagcgcct ttgagaatga cgcgatcgcc ttctttgacg tgatgaatcg ctgcggcgtt
      121 cttgccgagc ttgtcaatcc ggccgagaat ttcatgaccg agaatgatcg gataatcgac
      181 ctttaacttg ccgtgaagat atttcacgtc ggtgccgcag acgccgccca tctctactct
      241 caacagcccg ccgtccggcg gaatgtcggg catcggatac tcgcggacct cgagtttgtc
      301 ggggccgagc agtgtcgctc ctcttgaact tgccatggtt tgtcctcctt acctgtgctt
      361 tgtattcgag cagaagtctc tcgcccttgc tcgggatgaa gagacacggt tttaatccga
      421 tctcacttca cttcactcgg tgttcctgag aaactttgtc gatgaaaccg ctgctttcaa
      481 gttccttgac cagtgataaa tcggtgtaac ggtctgggtt ttcttttagt accttgggat
      541 ctttcttgcc gacttcttga atcggcagct cgaaatactc ccgtgaggga tagggtttga
      601 gcggaaaatc atactccttg aagttttctt ccagcacttc gcgatcctga actttcatgt
      661 atttcgacag cactttcatg gcgaattcct tattcgactt gtagagcgcc acgccctcga
      721 tcattgcttt ggtaaagttg accagctccg gtcgcatctt ccccaggtag gagcggcgcg
      781 ccacgaagaa aaggcttgag tagggaatgt tcaacttcga cgcgtcgagc agcagcgtca
      841 tgccgagttt gcgcgcctgc agggtcatcg gcggggtcag catggcggca tcggcattgt
      901 tcgtctcgac ggcggcaatg cggttggctt gattgccaac ttgcagcagc gcgacatcga
      961 tgtcgggccg cagcccctcg cgctggagca ggaagcgtgc cgcgaagtcg gcattgctgc
     1021 cgaaacgact gatggcgacg cgttttacct ccttgatatt tttgattcct ttacgcgcgt
     1081 agacgcggtt gtccatcgtg ttgcccaagc ggccgaacat gacgagatcg agctgcggcg
     1141 tcgagattac cacctggggc gaaaacatgc cgacatgcgc gtcgcccgag cttagcgcgg
     1201 cggccgctga cgatgcgccc tgaatgaaga tcagctgtac gttattgccg tacttttcga
     1261 aaagtttggc ttccttggtt acccaaaggg gcgcgaattg accgacctcg ctggtccaaa
     1321 cgacgttgat gttagccgcg ccaatcgtgt ttgtttggcc gcacatccac gcgataatac
     1381 tcagccgaac cattcgccgg acaatcgtca tgatccgctc cttggaggtg aatgtgccca
     1441 tagttgatct tgcttcccgc ttacccctat acttccgaag cgccattata atcaagttgg
     1501 aggaggagaa atgattcaat ccagtttggc cgtgttgttt ttattttggc ttgttagctt
     1561 tgcgtcggct cagtctccaa ctcgggtgat gtccggctac gccgcgacgt cgggaccgca
     1621 tgcggtattg tggattgcgc gcgacgccgg gttattcgag aaaaatggct tgcgcaccga
     1681 tgtcgcttat attcgcagcg gctcgaccat ggctcaaacc ttggtctccg gcgatatcca
     1741 aatgtcgcag atgggcggac cggccatgct cgccgccgga gttgcgggaa tggacgttac
     1801 ctttgtagcg gttgcactca acacgacacc gatcgtcttg atgggcacag tttccaaagt
     1861 cgaagagttg aaagggaaag cagtgggggt cacccgctac ggatccaaca ccgatatctc
     1921 ggcgcgcttt gcaattcgca aagccggact gcaaccggag aaggacgtcg cgttggttca
     1981 actcgaagac tatgccggca tcatgggcgg catcgcctct ggccggattg ccgctggggc
     2041 actcgccgac ccgtttaccg atgccgcgaa gaagctcggc tacaaggaag tcgccgatgt
     2101 tgctgctatg ggtcttgagt ttcctttcgt tgggattgcc gcaaaaaaat cttacatcaa
     2161 agacaacgtc gatactgtgc agcgttttgt acgggcctac accgaggcga ttgccatgta
     2221 caaaaataac cgcgatctcg ccatgaaaat caccggcaaa tacaccggca tcaaagatcc
     2281 ggctattctc gcatcgaccg tggattttta tgcgccgaag ttgcagcgag tgccattcgc
     2341 gacggtcggt ggtctgcgct tcgttctcga ccaagtagca gtgcgcgatc cgcgcgccaa
     2401 gaattacacc cccgaaacat ttatggatat gcgcttcgtc aagcagttgg aagatagcgg
     2461 ctttatcaaa gcgctttacc ccaaaggctg atgcgcctta acaattctgt gagatcacga
     2521 ttttattgcg cgcgagaaat aggcttgcga tgaatagcat tagcagcgcg ctggccatga
     2581 tcgttggaat caaatagtta atcgcgggga gaatcgccag cgccgccaaa ccgattccca
     2641 tcacgggtcg cacttgcggt ttgatcaaac taagcagtag cgcgcagggt atgatgagca
     2701 gagtcaaatc gtgagtaaat aagtgaggca gtacgatcat caggccaagg cagttcacga
     2761 tccagcgggt cgcgtgatcg tcgctatgct tggcctcggg ccaattgatc cagattaaaa
     2821 tcccgacaat taacgcgctt aggagccacc aagtcagcgc gtgccaagcc gtcggcaacc
     2881 cgtagatcac caatacgcgc agattatgca tgccacgcca ttgattccac cagtcgctat
     2941 ccgcgctgac catccggcgt gccagtcgaa aatattgcgt cgacgcctcg aagccaatcc
     3001 aaagaaatag gccgataatg agcacgctgg cgacggcggc gccgacgaga aattcgcgcc
     3061 agcgacgacg caccagcatg attaaatggg gaatcgccaa gtattgcggt ttgatacaaa
     3121 gcaatccggc gcaaactccc gcaaccgggc gctccgcttg cctctgggcc aggacatggg
     3181 cggtcagaaa aaatagcaca agggcggagg tttgaccgta gaaaattact ccgtgaacgg
     3241 cgaaattgca gagtgtaaac aacagcagcc aatgagattg atcgctggat agatgcaaac
     3301 cgcgaatcag taatcgcaag ctttggccaa tcagcagtag gttcacaagc gtcatgatca
     3361 agaatgccgt ggaaaattcg aaccaagcca acggcgcgag aaaggcggcg gtaatcggcg
     3421 gataaacgaa aggtaatact ctttccaccg gggcgatgcg gttttgatag agttgctggc
     3481 gatcgaggtc gtagagggat tgcggatcgt cccacatcaa ttgcgctgcc gcatagaatg
     3541 tgacgaaatc ggcaacacag ttatcggtgc tgcacttcgg gacatggtgg gcgtagatgt
     3601 tccaccaatt aaggctcatc aggaaagcga ggcccagccc gacgcccaca aagcccgcct
     3661 taatgcggtt gatttgactt gcactcgcca tagtggcgct ttagacgacg ttcggcgttg
     3721 gcgggtgtcc tttcgctcgt acgtttcagc ttcgtgacag cgccattcca agcaacactg
     3781 ccaggggacc gagtccggcg ttaagtttca ggagcacgaa ccagcggcgc cagtcgctca
     3841 gcagcgcttc gggccagacg gcggcgctgg ggttaagcgc tgctgtttgt cgagcaatgc
     3901 tcggtgcgta tttgaaaact tgcaggcagt gatgaaccgt catcgccata aaaacgaaga
     3961 gtttgccgcc gagaagggcc atgtacgcgc cgcccgttgt gacggcggac ggattgccga
     4021 gcgcgagatt ggcgatgcca gtgataagta gcacggcgat accgatccat gagatcgctt
     4081 cgagaccgat gcgacctcgg ttcatcgtgc ggatcgcgat gctcggttcc atatcatgca
     4141 ccgccgaacc catgacgaca agaaagaaaa ccatggcgcc aatccacatc gcaaacgcga
     4201 ttagatgcat tagcaaattt atcgtctgcc aagtagtcat ggtaaagtaa ggatgaacaa
     4261 gcgcagctct gtcagtagca tcggtgctaa accattgcaa atctcgggct attcttcttg
     4321 aaatggtact atcgctgtgt tagctagaac ctagtttttt tctcatactt ccataaattt
     4381 taaggatggt tggataatgc tgcgttggtt gccggaagat gtgtctacgt acggtcatga
     4441 gatcgactcg ttattttacc tgatctatta catcacggcg gtgacttttg tgctcgtcac
     4501 cgttttgatg gtttggtttt taattaaata tcgggaacag cccggacggc gcgcgatcta
     4561 taaccatggc aacaccacac tggaaatcat ctggacggtt atccccgcgg ccattttgat
     4621 cgtactgtcg ttcatgagcg tcaacacgtg ggctaagatc aagcgcaaca ttccggccag
     4681 cgattttgaa attcaggtca ccgccaagca gttcaactgg gatgtagttt atcccggcgc
     4741 cgacggtaag ttcggcacgg aggatgatgt caaattcgac aacgatcttc atgtgccggt
     4801 gaacaaggtg gttcgagttc atttaatgtc gagcgatgtc atccacagct ttttcattcc
     4861 caatctgcgt ttcaagcaag atgccgtgcc cggtcgtacg attttgacct ggttcgaagc
     4921 caagaaggca ggcaggtacg agttgccttg cgctgaactg tgcggcttcg gtcactccgg
     4981 catgaagggc tggctcaatg tccacacgcc ggaagattat caaaagtggg ctgcggagaa
     5041 tctcgcgccc aagccccaat agcacgtctc taaattagcc gaggattact acggaggttg
     5101 tcaagcgatg agcgagtcag cagtggcgca tggagcgcat ggacatcacg caaagccgag
     5161 tttcgtcagt cattatctat tttcactcga tcacaagatg atcgcccggc aatttctctt
     5221 tgtcggcctg ctaatgcttt tgatcggcgg tattctcgcc atgctggtgc gctggcagct
     5281 ggcctggccg gaaactccgg tgccgggcct cggttttatc tctgaacctt acatttatcc
     5341 gggtcccaac ggcggcggta ttattccgcc gcagacttat aacgcacttt ttacgatgca
     5401 tgcgacgatc atgatttttt tcgtggtcat gccaatcatg gtcggtacct tcgggaactt
     5461 tcttattcca ctgatgattg gcgccggcga catggcgttt ccgaaactca atatgttgtc
     5521 gttttggttc ggcgcagttt ccggagtcat catgctggcc agtttcttcg tacccggcgg
     5581 accggctgcc ggcggttgga ccggttatgc cacgctatcg gccagggcgc agtacaccgg
     5641 cgtcgagtgg gggcagcatc tttggatcat cgctttgatg gtcaacggta tttcgtcgtt
     5701 gatgggtgcg atcaattaca ttaccacgat cgtcaacatg cgcgccccgg ggatgggcta
     5761 ttttcgcatg ccgctgacag tctggtcgct gttcgtcacg gcaattcttt tgctgctcgc
     5821 gctgccggtc ttgacggccg cgctggcgat gttgttgttc gatcgcatgg ccggtaccag
     5881 ctttttcttg ccggaaggcg gcggtgagcc gctgctctg
//
LOCUS       DATLCH010000104        10387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9266533, whole genome shotgun sequence.
ACCESSION   DATLCH010000104 DATLCH010000000
VERSION     DATLCH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9266533"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VMZ02_03055"
     CDS             <1..198
                     /locus_tag="VMZ02_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70937.1"
                     /translation="EAIIHEARQQRETILAQAREEAARSLEQIRQEVAAALEREKHVA
                     AAEISSVAADMVNKILGRRVG"
     gene            195..764
                     /locus_tag="VMZ02_03060"
     CDS             195..764
                     /locus_tag="VMZ02_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HUR70938.1"
                     /translation="MSSHNLRELFTTAQLWASEAASHGAAEHHVPEVTDVIFPALNFL
                     IYAFIIVKYALPAVRSFLKSRREEVIATMSQASAKKAAAQALVDEYKAKLTAVDREIQ
                     SLQTTLREEGERDQVKLIADANALAAKIKDDARVLAEQEVKMARQKIREEMAMQAEAT
                     ARALLQRNLSIDDQNRLANEFIESIGRAR"
     gene            761..1303
                     /gene="atpH"
                     /locus_tag="VMZ02_03065"
     CDS             761..1303
                     /gene="atpH"
                     /locus_tag="VMZ02_03065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HUR70939.1"
                     /translation="MTEGSLARRYTKALFQLARDDGQEDAVGHEIEQFYADYSRSELQ
                     QVLINPAFPIDTRKQILDQVAHSARLSSLTVRFLSLLLERDRLGHLPGIVSCYRRLLN
                     EAKSRVDAKVVSATALDTAMVERVRGQLQTISGKEVILQQIVDSDLLGGLQVELEGKI
                     YDGSLRTQLEKMKQRIARGY"
     gene            1314..2822
                     /gene="atpA"
                     /locus_tag="VMZ02_03070"
     CDS             1314..2822
                     /gene="atpA"
                     /locus_tag="VMZ02_03070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HUR70940.1"
                     /translation="MEIGTAEISRIIKEQIRDYDKTVEIQEVGTVLSTGDGIARIHGL
                     DKVAAGELLEFPHGIFGIALNLEEDNVGAALFGETHMIKEGDTVKRTGRIAEVPVGKE
                     MIGRVVDALGAPIDGRGPIESKDRRRIELKAPGIVARQPVKEPLQTGLKAIDGMIPIG
                     RGQRELIIGDRQTGKTAVAIDAIINQKGGNVICIYVAIGQKRSTVAQVVDRLREAGAM
                     DYSIIVAATASESAPLQFIAPYSGCTMGEYFRDNGGHALVIYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKMSAERGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYSGVRPAINVGISVSRVGGNAQIKAMKQVAGTLRLDLAQYREM
                     AAFAQFGSDLDAATQKQLNRGARLVELLKQGQYQPLSVEQQVVILYAGTNGYVDDLPI
                     NSLKRFEQELFSFISSRHPDVFADVLTKRELDGDLRGKLNKVLEEFKGVFKA"
     gene            2849..3709
                     /gene="atpG"
                     /locus_tag="VMZ02_03075"
     CDS             2849..3709
                     /gene="atpG"
                     /locus_tag="VMZ02_03075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HUR70941.1"
                     /translation="MATLKVIRKRIGSVRNTQQITKAMKMVSAAKLRRAQEAAVQARP
                     YAEKMTELLKNVAARVASEAHPLLAPREEKKVQLVLFTSDRGLCGGYNTNMIRAAEAF
                     IRQHAGDRQFQLSLVGRKGADHFRRRGADISERYVDILYKAADELAGEIVDKLIKRFV
                     NGEVDAVYIVYSRFRSALSQVPTVEKLLPVTVAESIDAQQQTEYLYEPGVEQLLASLL
                     PRITGVAVQRALLEATASEHGARMTAMESASGNAAKMIGSLTLQMNRARQASITRELL
                     EIVGTAEALK"
     gene            3795..5204
                     /gene="atpD"
                     /locus_tag="VMZ02_03080"
     CDS             3795..5204
                     /gene="atpD"
                     /locus_tag="VMZ02_03080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HUR70942.1"
                     /translation="MSTGKLTQVLGAVVDVEFSDGALPPIYNALKITNPAISDAQWNL
                     VVEVAQHLGENTVRCVAMDTTEGLVRGMDVLDTGAGISVPVGEETLGRILNVIGEPVD
                     EMGPVNAKKSSPIHRSAPEFVDQETKVQALETGIKVVDLLAPYARGGKVGLFGGAGVG
                     KTVILMELINNVAMKHGGYSVFGGVGERTRVGNDLWLEMKESGVINKASLVYGQMNEP
                     PGARARVALTALTLAEYFRDDEGRDVLLFLDNIFRFTQANSEVSTLLGRMPSAVGYQP
                     TLSTDLGELQERITTTRKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRQIAE
                     LGIYPAVDPLDSTSRILDPLVVGQEHYEVARSVQLLLQRYKDLQDIIAILGMDELSED
                     DKLTVARARKVQRFLSQPFHVAEQFTGIPGAYVELKDTIRAFQEVVAGKHDDIPEQAF
                     YMVGTIEQVQEKAKKMAAA"
     gene            5291..5707
                     /locus_tag="VMZ02_03085"
     CDS             5291..5707
                     /locus_tag="VMZ02_03085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HUR70943.1"
                     /translation="MADKIILRVVTPSRLVLEEEVDEVTAPGELGEFGVLPNHIAFLS
                     TLVPGELSYKQGAVRHSLVVAGGYAEVLENVMTVLAPAAEFPGEIDTARAQRAKERAE
                     KRKGELNFDDPEYAQVEEALQRALARLQVASKETRR"
     gene            5745..6878
                     /locus_tag="VMZ02_03090"
     CDS             5745..6878
                     /locus_tag="VMZ02_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR70944.1"
                     /translation="MAEAAKQEPVITMWSKEFTYDRWMKSKGIPIHTGYFIPDLRTVE
                     LEWWEERGCKAAFIQLLGQEGVSASRVTEVPPGKSLPPVKFALDEIVYVLSGRGLTTV
                     WAGEGKAKKNFEWQEHSMFLVPRNHWHVFSNMTGDKPVKLFHYSYMPMAMSALPEPDM
                     YFNNPYEPKITADQGDFYSEAKMVQAKDADEGLGMRAFWYGNFFPDMRAWNKLDANER
                     RGAGGKSVFMQFPNSELTAHMSVFAPQTYKKAHRHGPGRAIIIPAGEGYSIMWEEGKE
                     KVVCPWHECSMVTPPNKWFHQHFNAGGDMARYLALHPPMQFHGHAEKVEDRAKDQIEY
                     PNEDPFIRQKFEEELGKRGIKSIMPDECYKNPEYEWSKAMGKQ"
     gene            6957..8501
                     /locus_tag="VMZ02_03095"
     CDS             6957..8501
                     /locus_tag="VMZ02_03095"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HUR70945.1"
                     /translation="MADTIQRLQMHDDIYDHDHPDNFIIKEDEIVTDIEGLEMFSLKS
                     VGIDIGSSTSHLIFSHITLRREGASLSGKFRVTGREVLYRSPIMLTPYISATKIDTDK
                     VNDFIHNAYKEAGLTPEDVDTGAVIITGEALKKENAQPIVENFAKYSGKFICAAAGHN
                     HEALLAAYGCGAVDLSKGEHKTVLNVDMGGGTTKFSLVEEGVVTQTASISIGARLIAF
                     DDKDQITRIEDPGRTMMNALGHKVELGQKITEKMKEDFGAYMAKILFEIIEQGPMSPM
                     AKSLMVTPPFVNYTGFKQVQHLVFSGGVSEHVYDRDPKAYGDIGPVLGKNMRDYLKKL
                     PKDVLREPIEGIRATVIGAGEYTIQASGNTSYVSNEKALPVHGLKVIQAPVRDGESVA
                     DAIKQALRKFDLSSFTSGLALSLTVSGVPDYQSVRRIAEGVAQILKDADDQKCPLYLT
                     LDLDIAKSLGAILREELNVARDIIAIDGIEVGDLDYIDIGECLGITEVIPVTVKSLMF
                     PSTSGD"
     gene            complement(8663..9214)
                     /locus_tag="VMZ02_03100"
     CDS             complement(8663..9214)
                     /locus_tag="VMZ02_03100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR70946.1"
                     /translation="MTQFGHKLKLILLMLCLSGCGSSVALWTAGEVPFVRSAPEVIER
                     MLEVARVKPGDVIYDIGSGDGAILIYAAKKYGVKGVGIEIDQNLVDRARSNAYIENVQ
                     HLTEFRAQDAFTVDISPATVVTLYMLPEFNAKLRPIMERQLKPGTRIVSHDYAIEGWT
                     PQYAEQVRGTFVHDHSVLLFEIP"
     gene            9362..10084
                     /locus_tag="VMZ02_03105"
     CDS             9362..10084
                     /locus_tag="VMZ02_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HUR70947.1"
                     /translation="MASWDDILTPRDKEVFALSGFGKKAGLGQRPALLIIDVNYNFTG
                     DKPEPILESVKRFRTSCGEEGWDGVHAIRELLREARKKHVPTFYTTAEENRANSAVLV
                     GGWAAKSNRTTEDMTERWEKANQIVDEIAPQPGDIIVRKQKPSAFFGTPLMSMLTEVH
                     ADSLLVCGTTTSGCVRASVIDAFSYNLKVSVVEECVFDRGQASHKINLFDMAMKYADV
                     IPLTEAIDYVRALPTNLYAPAL"
     gene            complement(10128..>10387)
                     /locus_tag="VMZ02_03110"
     CDS             complement(10128..>10387)
                     /locus_tag="VMZ02_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70948.1"
                     /translation="FVKSVATIFMITKATQLIAVSTWNLFNKDTLTLSLQVVLFTLVG
                     FFAGLKTQDRVNQKTFSRGLMVLLSVVGVILIARSLSQALQ"
BASE COUNT         2451 a         2757 c         2969 g         2210 t
ORIGIN      
        1 gaagcgatta tccatgaggc gcgccagcag cgcgaaacga ttctcgccca agcccgtgag
       61 gaggcggccc ggagtcttga acagataaga caagaagttg ccgccgccct agaacgcgaa
      121 aagcatgtcg cggccgctga aatcagttcg gtcgctgcgg acatggtgaa taaaattctc
      181 gggaggagag tcgggtgagc agccataatc ttcgcgaact attcaccacg gcacaattgt
      241 gggcttcgga agcagccagt cacggcgccg ctgagcatca cgttccagaa gtcaccgatg
      301 tcatcttccc agcgcttaat tttcttattt acgccttcat tatcgtcaaa tacgcgctcc
      361 cggcagtgcg cagtttttta aaaagccggc gcgaggaagt gatcgcgacg atgtcgcaag
      421 cctcggcaaa gaaggccgcg gcgcaggcgt tggtcgacga atacaaggcg aaactcacgg
      481 cggtggaccg ggaaatccaa agcttgcaaa cgactctgcg cgaagagggc gagcgcgatc
      541 aagtgaagct gatcgccgat gccaatgcct tggcggccaa gatcaaggac gacgcgagag
      601 ttctcgccga gcaagaagtc aaaatggccc gccagaaaat tcgcgaggaa atggcaatgc
      661 aggcggaagc cacggcgcgg gcgttgctcc agcgtaactt gtcaatcgac gatcaaaatc
      721 gcttggcgaa tgaatttatt gaaagcattg ggcgggcgcg atgaccgaag gcagcttagc
      781 acgcagatac accaaggcat tgttccaatt ggcgcgcgac gacggtcaag aagacgctgt
      841 cggccatgag attgaacaat tttacgccga ttatagccgc tccgaattac agcaggtact
      901 gatcaatccg gcattcccga ttgacacgcg taaacaaata ctcgaccagg tggcgcacag
      961 cgctcgcctg tcgtcactga ccgtaagatt tttgtccctg cttctggagc gcgaccgctt
     1021 gggccacctg cccggtatcg ttagctgcta tcgccgattg ctcaacgaag ccaagagtcg
     1081 ggtggacgcc aaagtggtta gcgccaccgc gctcgatacg gcgatggtcg agcgggtgcg
     1141 tggtcaactg cagaccattt ccggtaaaga ggtaattctt caacagatag tcgactcaga
     1201 cttactcggc ggcctgcaag tggagctcga gggaaagatc tacgacggca gcctgcgtac
     1261 gcaattggaa aagatgaaac agcgcatcgc gcggggctat taaggagcac gagatggaga
     1321 ttggaacagc ggaaatcagc cgaatcatca aagagcagat tcgcgattac gataagacgg
     1381 ttgaaatcca agaagtcggc acggtgcttt ccaccggcga tggtatcgcg cgcattcacg
     1441 gcctcgacaa agttgctgcc ggtgagctgt tggaatttcc tcacggcata ttcggtatcg
     1501 cgttgaacct ggaagaagac aatgtcggtg ccgcgctgtt cggcgaaacc catatgatca
     1561 aggaaggcga tacggtcaag cggaccggcc gcattgccga ggttccggtc ggcaaggaaa
     1621 tgatcggccg tgtcgtcgat gcgctggggg cgccgatcga cggtcgcggc ccaatcgaaa
     1681 gcaaagatcg ccgccgcata gaactgaaag ctcccggcat cgttgcccgc caaccggtga
     1741 aagaaccgct gcaaaccggt ctgaaagcga tcgacggcat gatccccatc ggccgcggcc
     1801 agcgcgagct gatcatcggc gaccgccaaa ccggtaaaac cgccgtcgcc atcgacgcga
     1861 tcatcaatca aaaaggcggc aacgtcattt gtatctatgt ggccatcggt caaaagcgct
     1921 ccacggtcgc ccaagttgtc gatcggcttc gagaggccgg cgcgatggac tattccatta
     1981 tcgtcgcggc gacagcgtcc gagtcggcgc cgctgcagtt catcgccccc tattcaggat
     2041 gtaccatggg cgagtacttc cgcgacaatg gcggccacgc cttggttatt tacgacgatc
     2101 tatcgaagca cgcggttgct tatcgccaat tgtctttgct gctacgacgg ccgccgggac
     2161 gtgaagccta tcccggtgac gttttttatc ttcattcccg cttactcgag cgcgccgcca
     2221 agatgagcgc tgaacgcggc ggcggttcac taactgcgtt gccgatcatc gagacccaag
     2281 ccggcgacgt ttcggcttac atcccgacca atgtgatttc gatcaccgac ggtcagattt
     2341 ttttggaaag cgatttgttc tattcaggcg ttcggccggc gatcaatgtc ggcatctcgg
     2401 tttcccgagt cggcggcaat gcgcaaatca aggcgatgaa acaagtcgcc ggtacgctgc
     2461 gtttggattt agcgcagtat cgcgaaatgg ccgcttttgc tcaattcggt tccgatctgg
     2521 atgcagcgac acagaagcaa ttaaatcgcg gcgcgcgttt agtcgaatta ctcaagcagg
     2581 gccagtacca gccgctgtcg gtcgagcagc aggtggtgat cctttatgcc ggcaccaatg
     2641 gctatgtcga cgatctcccg atcaattcac ttaaacggtt cgaacaagag cttttttcgt
     2701 tcatcagttc acgtcatccc gacgttttcg ccgacgtttt gacaaagcgc gaattggacg
     2761 gcgatctacg tggcaagctc aacaaagtcc tggaagagtt caagggagtg ttcaaagcgt
     2821 agttgcgagt tttcgaaatt tcaccgttat ggcaaccctc aaagttatac gcaaacggat
     2881 cggctcggtg cgcaacaccc agcagatcac caaagcgatg aagatggtct cggcggccaa
     2941 gctgcggcgc gctcaggaag ccgcggtcca ggcccgcccg tacgcggaga agatgaccga
     3001 gctgttaaag aatgttgccg cgcgggtagc cagtgaagcc catccgcttt tggcgccgcg
     3061 cgaggaaaaa aaagtccagc ttgtgctatt cacctccgat cgcggcttgt gcggtggcta
     3121 caacaccaac atgattcgtg ccgcagaagc atttatccgt cagcatgccg gtgatcggca
     3181 gtttcaattg tcattggttg ggcgcaaggg tgccgaccat tttcgccgcc gtggcgcgga
     3241 catatcggag cgttacgtcg atattctcta caaagcggcc gatgaattgg ccggcgagat
     3301 cgtcgataaa ctgattaagc ggtttgtcaa tggggaagtg gacgcggttt acatcgtata
     3361 cagtcgtttt cgttcggcgt tgtcgcaagt gccaacggtg gaaaagttgc taccggtgac
     3421 agtggccgaa agcattgacg ctcagcagca gacggaatat ctgtacgagc ccggtgtcga
     3481 gcaattattg gcgagcttat taccgcgcat caccggtgtg gcggtgcagc gggcactttt
     3541 ggaagccact gctagcgaac atggcgcgcg catgacagct atggaatcgg cgagcggtaa
     3601 cgccgccaaa atgatagggt ctttaacgct gcaaatgaac cgggcacgcc aggcttcgat
     3661 cacccgcgag ctgttggaaa tcgtcggtac ggcggaagca ctgaaataac taccgtgatc
     3721 gtctcgttaa ccgccggggc acgtacttac gagcacgaac atgattcgag gaatgattcg
     3781 gaggtcttaa tacaatgagt acgggtaaac tcacccaagt cttgggtgcg gtcgtcgatg
     3841 tcgaattttc cgacggcgca ttgccaccga tttataatgc gctcaagatc acaaatccgg
     3901 cgatcagcga tgcgcaatgg aatctggttg tcgaagtggc gcagcatttg ggtgagaaca
     3961 ctgtgcgctg tgtcgccatg gataccacgg aaggtttagt tcgtggcatg gacgtgctcg
     4021 ataccggagc cggcatcagc gtgcccgtgg gcgaagagac tctgggacgg attctcaatg
     4081 tcatcggcga gccggtggac gaaatgggtc cggtcaatgc caagaaaagc tcgccgattc
     4141 atcgctctgc ccctgaattt gtcgaccagg aaaccaaagt tcaagcgctt gaaaccggca
     4201 ttaaagtcgt cgacctgctg gcaccctatg cgcgcggcgg gaaagtcggc ctattcggcg
     4261 gcgctggagt tggcaagacc gtcattctca tggagttgat caataacgtc gccatgaagc
     4321 atggcggtta ttccgtcttc ggcggcgttg gcgagcgtac ccgcgtaggc aacgatctct
     4381 ggcttgaaat gaaggagtcc ggcgttatca acaaggcctc gctggtctac ggtcagatga
     4441 acgagcctcc tggagcgcgc gcgcgcgtcg ccttgaccgc cttgaccttg gctgagtact
     4501 tccgcgatga cgaagggcgt gacgtgctgc tgtttttgga taacattttt cgtttcactc
     4561 aggcgaactc ggaagtctcc accctgctcg gccgtatgcc ttctgccgtc gggtatcagc
     4621 cgaccctatc gaccgatttg ggcgagttgc aagaacgcat cacgaccacg cgcaaaggtt
     4681 cgatcacttc cgttcaagct atttatgtcc cggccgacga tctgaccgac ccggcgccgg
     4741 cgaccacttt cgcacatttg gacgcgacca ccgttctttc ccggcaaatc gccgaactgg
     4801 gcatttatcc ggccgtcgat ccgctcgact cgacctcgcg tattctcgat cctttagtgg
     4861 tcggccagga acactatgag gttgcccgat cagtgcagct cctgttgcag cgttacaaag
     4921 atttacagga catcatcgcg attctcggca tggacgagct ttccgaagac gataaattga
     4981 ccgttgcgcg ggcccgtaaa gtgcagcgct ttctgtccca gccgttccac gtcgccgagc
     5041 agtttaccgg cattccgggc gcctacgtcg agttgaaaga cacgatccgc gcgttccaag
     5101 aagtagtcgc cggcaaacac gacgacattc ctgaacaggc tttttacatg gtcggcacca
     5161 tcgagcaggt ccaagaaaaa gccaagaaga tggccgcagc ttagctcagg catggggcgt
     5221 gagccatgag gcatgagtca gattctcgac tcgcgccttg cgtctttcgc cgcacgcctt
     5281 ggtatttacc atggcagaca agattatact gagagtcgta acaccgagcc ggctggttct
     5341 cgaagaggag gtcgacgagg tcaccgctcc cggcgaactt ggcgagtttg gcgtcctgcc
     5401 caatcacatt gcgttcttgt cgacgctggt tccaggcgaa ttgagctaca aacaaggcgc
     5461 tgtgcgtcat tcattagtcg tcgccggcgg gtatgctgag gttttggaga acgtcatgac
     5521 cgtgcttgcc ccggcggcgg agtttcccgg cgaaatcgat acggcgcggg cgcagcgggc
     5581 caaagagcga gccgagaagc gcaagggcga gctaaacttc gacgaccccg agtatgccca
     5641 agtggaagag gcgcttcaac gcgccttggc ccggctccag gttgctagta aagaaacaag
     5701 acgataagaa tgaaagttat gagaagtagc aaggaggaag gctaatggct gaagcagcta
     5761 aacaagaacc cgttataacc atgtggagca aggaatttac ctatgatcgg tggatgaagt
     5821 cgaaagggat tccgatccac acgggctatt ttatcccaga tctccgcacc gtcgagttgg
     5881 aatggtggga agaacgcggc tgcaaagcgg cttttattca gctcttgggc caagagggcg
     5941 tcagcgcatc gcgcgtcacc gaagttcctc ccggcaaatc tctaccgccg gtcaaattcg
     6001 ctctcgatga aattgtttat gtgctttccg gccgtggttt gactaccgtc tgggctggcg
     6061 aaggcaaggc gaagaaaaac tttgaatggc aggaacattc aatgttccta gttccacgca
     6121 atcactggca cgtgttcagc aacatgaccg gcgataagcc ggtcaagctt ttccactaca
     6181 gctacatgcc gatggcgatg tccgcactgc ccgaacccga catgtacttc aataatccct
     6241 atgagccgaa aataaccgcc gatcaaggcg atttttattc cgaagcgaag atggttcaag
     6301 caaaagatgc cgacgaaggc ttgggcatgc gcgccttctg gtatggtaac ttctttcccg
     6361 acatgcgcgc ctggaataag ctcgatgcca acgaacggcg cggcgcgggc ggcaagagcg
     6421 tgttcatgca gtttccaaat tccgaactta ccgcacatat gtcggtattc gcgcctcaga
     6481 cttacaagaa ggcgcatcgc catggtccgg gccgcgccat catcatcccc gcgggcgaag
     6541 gctactcgat catgtgggaa gaaggcaaag aaaaagtcgt ctgtccatgg catgaatgtt
     6601 ccatggtgac gccacccaac aagtggttcc accagcattt caatgccggc ggcgatatgg
     6661 ctcgttacct ggcactgcat ccgccaatgc agttccacgg ccacgcggaa aaggtcgagg
     6721 accgggccaa ggatcagatc gaatatccta acgaggatcc gtttattcgt cagaaattcg
     6781 aggaagagtt gggcaagcgt ggcatcaaga gcattatgcc agatgagtgc tacaagaacc
     6841 ccgagtacga atggtccaag gcaatgggca agcaataata cagtaactgc ttaaaagacc
     6901 gactacggaa tccccgctcc aaggagcggg gattcctgtt attagggtag gagaaaatgg
     6961 cggacactat acagaggcta cagatgcacg acgatatcta cgatcacgat catcccgata
     7021 attttatcat caaagaggat gagatcgtta ccgacatcga gggtttggag atgttttcgc
     7081 tgaaaagcgt gggcatcgac atcggttctt ccacatcgca cttgattttc tcgcacatca
     7141 cgctgcggcg cgaaggcgcg tcgttatccg gcaagttccg ggtcaccggt cgcgaggtgc
     7201 tctatcgctc gccgatcatg ctgactccgt atatctcggc gacaaaaatc gacaccgata
     7261 aggtgaacga ttttattcac aacgcttaca aagaggcggg cttgacgccg gaagacgtcg
     7321 ataccggcgc ggtaatcatc accggcgagg cgctcaagaa agaaaatgcc caaccgatcg
     7381 ttgagaactt cgctaaatat tccggcaagt ttatctgcgc ggcagcgggg cataaccacg
     7441 aggcgttgct agccgcttat ggctgcggtg cagtggattt gtcaaagggc gagcacaaga
     7501 ccgtgctcaa cgtcgacatg ggcggcggca cgacgaagtt ttcgcttgtc gaagaaggcg
     7561 ttgtgacgca aaccgcctcg atcagcatcg gcgcgcggct catcgcgttc gacgataagg
     7621 accagattac ccgcattgag gatccgggcc ggaccatgat gaatgcgctt ggacataagg
     7681 ttgagctcgg gcagaaaatt actgagaaaa tgaaagaaga cttcggcgcc tatatggcaa
     7741 agattctctt cgagatcatc gagcaggggc ccatgtcgcc gatggcgaag tcgctcatgg
     7801 ttactccgcc cttcgtcaat tacaccggct tcaagcaagt gcagcatctg gtcttctcgg
     7861 gtggcgtttc cgagcatgtc tatgatcgcg atcccaaagc ctacggcgat atcggtccgg
     7921 tgctcggcaa gaatatgcgc gactatctaa aaaagctgcc gaaggacgtg ctgcgcgagc
     7981 ccatcgaagg tattcgcgcc acggttatcg gcgccggtga gtacacgatc caggccagtg
     8041 gcaacaccag ctatgtttcc aacgagaaag ccctgccggt acatggcttg aaggtcattc
     8101 aagcaccggt ccgcgacggt gagtccgtgg ccgacgcgat caagcaggcg ctcagaaaat
     8161 ttgacctgag cagcttcacg tcaggtttgg cgttgtcttt aactgtgagc ggcgtgcccg
     8221 actaccaatc ggtccggcgc atcgccgaag gcgtggcaca aattctcaaa gacgctgacg
     8281 atcaaaaatg cccgctctat ttgaccttgg acttggacat cgccaagtca ctcggcgcga
     8341 tcctgcggga ggaactcaac gttgcgcggg atattatagc gatcgacggc atcgaggtcg
     8401 gtgatttgga ctatatcgac atcggcgaat gcttgggcat caccgaagtc attccggtta
     8461 ccgtcaagtc attgatgttt ccaagcacgt cgggcgacta atttcgttat caggtgcggt
     8521 agtcaaaagc ctcgcccgtg cggggctttt ggcgttcggc tgtccagtta ccaacttcga
     8581 gcgggaggaa gaacggacaa cctcggcggc ggctgtaccg cgcccgttcg tcccttgtta
     8641 gatacgatct caggtgcaaa ttctagggta tctcaaagag aagcaccgaa tggtcatgaa
     8701 cgaaggtgcc gcgaacctgt tcggcatatt gcggagtcca tccttcaatc gcgtagtcgt
     8761 gggaaacaat tctggtgccg ggcttgagct ggcgctccat gatagggcgt agcttggcat
     8821 tgaattccgg cagcatgtag agagttacaa cggtggctgg agaaatgtcg acggtgaaag
     8881 cgtcttgggc gcgaaactcg gttagatgtt ggacgttttc gatataggca ttactgcgcg
     8941 cgcggtcgac taaattctgg tcgatttcga taccaacgcc tttgacgccg tattttttgg
     9001 ccgcataaat aaggatcgcg ccgtcaccac tgccgatgtc gtatatgaca tctccaggtt
     9061 taacccgcgc cacttcgagc atgcgttcga tcacttcggg ggccgatcgc acgaagggca
     9121 cctcgccggc ggtccacagc gcgactgaag agccgcagcc gcttaagcat agcattagta
     9181 aaatcagctt cagcttgtga ccgaattgtg tcattgctgt gacatacctc gtagatcagt
     9241 agtgagctcc ccatgttata ggcgtcagtt gattgggcgt caatgcgatg aggattatca
     9301 tcttgacatc gccgtcggtt tataatgaac attcggtgaa tacttaattg gaggaattct
     9361 catggccagc tgggacgata tcttgacgcc gcgcgataaa gaagtcttcg cgctttccgg
     9421 tttcggtaag aaggcgggtt tgggtcaacg gccggcgctg ttgattatcg atgtcaatta
     9481 taatttcacc ggcgataaac ccgagccgat tctcgagtca gtaaagcgct ttcgcaccag
     9541 ctgcggtgaa gaaggttggg acggcgtcca tgcgattcgc gagttgcttc gcgaagctcg
     9601 taaaaagcat gtgccgacgt tttatacgac tgccgaggaa aatcgcgcca acagtgcggt
     9661 tttggttggt ggctgggcgg ccaaaagcaa tcgcactacg gaagacatga ccgaacgatg
     9721 ggagaaggcc aatcagatcg tcgacgagat cgcgccacag ccgggcgata tcatcgtgcg
     9781 caagcaaaaa cccagcgcct tcttcggtac gccactgatg agcatgctga ccgaggttca
     9841 tgccgacagt ttgctcgtct gcggcaccac caccagcggt tgcgttcgcg ccagcgtcat
     9901 cgacgccttt tcttacaact tgaaagtctc ggtcgtcgaa gaatgtgttt tcgaccgcgg
     9961 tcaggcttcg cacaaaatca atttatttga catggcgatg aagtatgccg acgtgatccc
    10021 gctcacagag gcgatcgatt atgtccgtgc tttgccgacc aatctttacg cgcctgccct
    10081 gtgaagcaag tttctcacgt tgcgcgatcg accaagctaa tgacagacta ctgcagggct
    10141 tggctgagcg agcgggcgat gaggatcacg ccgacgacag atagcagcac catcaatccg
    10201 cggctaaagg ttttttggtt cacccgatcc tgagtcttta aaccggcgaa aaagccgacc
    10261 aacgtaaaca gcaccacttg taacgataga gtgagggtat ccttgttaaa cagattccac
    10321 gtcgagaccg cgattaattg agttgccttg gtgatcatga agatcgtcgc gaccgatttg
    10381 acgaagt
//
LOCUS       DATLCH010000105         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9857743, whole genome shotgun sequence.
ACCESSION   DATLCH010000105 DATLCH010000000
VERSION     DATLCH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9857743"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VMZ02_03115"
     CDS             complement(<1..1191)
                     /locus_tag="VMZ02_03115"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR70949.1"
                     /translation="MFEDLRGFLAHLEDLGQLKLIKDEVSVKHEIAAGMRKTSDIEGP
                     ALLFENVKNYPDWRVLGGLFATRKLVSLGLGVPQDEMLDRYMTLEDKRIPPEIVTTGP
                     CKEIKWIGDQVDLSKLPIVTHASKDCGPYVTIGMQIGKDPDTGIRNLSIHRMLVLGKD
                     TLSLWAPADHHLGRMILKAEENGKGLPVATAVGVEPSIVVASQARVPYGIDEFHVAGG
                     LRGAAVKLVKCETIDVEVPAMAEVIIEGITIPGERVPDGPYGEYPGCYSDAKQSPVLK
                     VTAITMRKNPIWQTALTGMPVTENHTLIEYGNAAVVYREVKKIVPEVKAVNMTPGGTF
                     RHHVVVSIKKRAENEGRNVILALLSMGIGLKQVIVVDDDIDAFDPMQVDWAMATRFQA
                     DRDLI"
     gene            complement(1268..>2126)
                     /locus_tag="VMZ02_03120"
     CDS             complement(1268..>2126)
                     /locus_tag="VMZ02_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR70950.1"
                     /translation="DSLIHKPRLEAITMLAAVATHTKTVRLGTMCMASFPVRHPVLLA
                     IQWASLDQLSQGRSLLGVCIGGGHEGELRAFGIRREERVGRMREGIELLRQIWSDEST
                     SFRGKYYNLENYNIVPKPVQKPPPIWIAVSPDREQVGDRIVDQAMRRVGLLADGYITM
                     GVTAEELGKRLAIIEHTAQQIGKDLSNFEIAIHGMININDDKRLAYRQSKDYFNHYYG
                     PAYPPESLIKVWLAHGPAKDCAQLIQEWIDMGITTPVLRFTSKDQAGQLKRFVEDVLP
                     LLRLKTRLQ"
BASE COUNT          446 a          637 c          542 g          501 t
ORIGIN      
        1 gatcaagtct ctatccgcct gaaaacgcgt cgccatcgcc cagtcgacct gcatcggatc
       61 gaacgcatcg atgtcatcgt cgacgacgat cacttgtttc aaaccgatgc ccatcgatag
      121 caacgctaga atgacgttac gtccttcgtt ctccgcgcgc tttttgatcg aaaccacgac
      181 gtgatgtcgg aaagtaccgc cgggtgtcat attgaccgct ttgacttcag gcacgatctt
      241 tttaacttcc cgataaacca ccgcggcgtt gccgtactcg atcagcgtgt gattttccgt
      301 caccggcatg ccggtgagcg cggtctgcca aatcgggttc ttgcgcatgg taatcgccgt
      361 gacctttaac accggcgact gcttggcgtc gctgtaacac ccggggtact ccccgtacgg
      421 tccgtccggc actcgctcgc cgggaatcgt aatgccttcg atgattacct cagccatcgc
      481 cggaacctcg acgtcgatcg tttcgcattt caccagcttt accgcggcgc cgcgcaggcc
      541 gccggcgaca tgaaattcgt cgattccgta cggcacccgt gcttgcgacg cgacgacgat
      601 cgacggttcg acgccgaccg cggtggcaac cggcaaacct ttgccgtttt cctcagcctt
      661 taaaatcatc cgaccgagat gatgatccgc cggcgcccac aacgataacg tgtccttgcc
      721 cagaactaac atgcggtgga tggaaagatt gcgaatgccg gtgtccggat ctttaccgat
      781 ctgcatgccg attgtgacgt agggtccgca atctttgctg gcatgggtga cgatcggcag
      841 cttgctgaga tcgacttggt cgccgatcca tttgatttcc ttgcacgggc cggtagtgac
      901 aatttccggc gggatgcgtt tatcttcaag ggtcatgtag cgatcgagca tctcgtcctg
      961 cggcacgcca agaccgagcg aaactaactt tcgcgtcgca aacaaaccac cgagcacccg
     1021 ccaatctgga tagttcttga cgttctcaaa aaggagcgcc gggccttcga tgtcggaggt
     1081 cttgcgcatg ccggcggcga tctcgtgctt gacgctgacc tcgtctttga tcaacttcag
     1141 ctgccctaag tcttccagat gcgcgagaaa acctctgaga tcttcaaaca tgaaataacc
     1201 cccacgagat aattcgaatg atgcaatatt gggttggcaa tccgtaaccc atttctccat
     1261 cactccatta ctgcaacctc gttttcaatc ggagcagcgg caacacatct tcgacaaatc
     1321 gcttcaactg tcccgcttgg tccttggagg tgaagcgcag taccggcgtc gtgatgccca
     1381 tgtcgatcca ttcttgaatt agctgcgcgc aatccttcgc cggaccgtgg gccagccaga
     1441 ctttaatcaa gctctcgggc ggataggccg ggccgtagta atgattgaag tagtctttcg
     1501 actgccggta ggcgagcctt ttatcgtcgt tgatgttgat cattccatga atggcgattt
     1561 caaagttgga caaatcttta ccgatttgtt gtgcagtgtg ctcaataatt gccaggcgct
     1621 tgcccaattc ttcggcagtg acgcccatcg taatgtagcc gtcggcgagc aatccgactc
     1681 ggcgcatcgc ctggtcgaca attctgtcac cgacctgctc ccggtcggga ctcaccgcga
     1741 tccagatcgg cggcggcttt tgcaccggct tgggcacgat attgtagttt tcgagattgt
     1801 aatacttgcc gcgaaacgat gtgctctcat cgctccaaat ctgccgcaac aactcgattc
     1861 cctcgcgcat acggccgacg cgttcttctc ttctaattcc aaacgcgcgc agttcgcctt
     1921 catgaccgcc gccgatgcag acaccgagca gcgacctgcc ttgcgagagc tggtccaacg
     1981 acgcccactg aatcgccaac aaaaccggat ggcgcacagg aaacgacgcc atgcacatag
     2041 tgccaagccg aaccgttttt gtatgcgtcg ccaccgccgc gagcatggta atcgcttcga
     2101 gccgtggttt atgaatcaaa ctatcg
//
LOCUS       DATLCH010000106         5858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5554413, whole genome shotgun sequence.
ACCESSION   DATLCH010000106 DATLCH010000000
VERSION     DATLCH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5554413"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="VMZ02_03125"
     CDS             <1..1154
                     /locus_tag="VMZ02_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUR70951.1"
                     /translation="AWMGFEARHAVLEVLDKAIADKKAQVRVIAYDLSEPDVVSRLER
                     LGSRLKILIDDSDKHGNAGSGETQSAQRLTISAGAANVKRQHMGQLQHNKTIVVDGAK
                     FKAALCGSTNYTWRGFFVQANNAIILRGRNAIKPFVAAFDDYWNHQTPGKFANTASAA
                     WTSLGVDGVTAKVAFSPHSVANSLLQTIADDIATKTTSSLFYSLAFLYQTPGVMLDAI
                     KKVSANDKLFVYGISDRKVGGLDLQKPDGNVAAVFPSALNTNLPEPFKSEPVGGSGNR
                     MHHKFLVVDFNKPGARVYMGSYNFSGTADTKNGENLLLIRNRRIAVSYMIEALRLVDH
                     YHFRVAQQEAQRARTMLRLEKPPRLPGETPWWSKYYTNPLKIRDRELFG"
     gene            1288..2391
                     /gene="ada"
                     /locus_tag="VMZ02_03130"
     CDS             1288..2391
                     /gene="ada"
                     /locus_tag="VMZ02_03130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HUR70952.1"
                     /translation="MNLSNNNNRGKSVNRTDSRWPFLMRRDRAADGKFFYSVQTTGVY
                     CRSSCAARRPRPENVQFYATCAEAEQAGFHPCRRCKPNQPSESAGHAAKITGVCRLIE
                     TSEELPSLAVMAKHAGLSPHHFHRVFASLTGVTPKAYGVAHRAKRMRAELRRSSSVTE
                     AIYGAGYNSSGRFYECSDKALGMKPMQFRAGGADTNIRFAVGACSLGAVLVAASDRGV
                     CAIFLGDDSEALVRELQDRFPRAELIGGNAEFELLVAKVVGYIDDPALGIDLPLDIRG
                     TAFQQRVWQALTKIPAGSTATYMEIAQQIGMPKSARAVARACATNLLAIAIPCHRVVR
                     TDGSLSGYRWGIERKRTLIEKESGSQSRVPRHA"
     gene            complement(2393..3517)
                     /locus_tag="VMZ02_03135"
     CDS             complement(2393..3517)
                     /locus_tag="VMZ02_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70953.1"
                     /translation="MHIRFALFVLCVFTSTPVGFGAEVKPAWQSSWEQTIAAAKKEGR
                     LNFYVGRYGSEPLLNEFRKEFPEIKIVTVNGAGNSLGTRIVTEIRAGHVVADLFSGGA
                     NTNYEVLYEGKALDSIKSLLVLPEVLDESKWYEGRIRFTDPEQRHVLVYIANPTSSGF
                     YYNTTLVNPKEFKSYWDLVAPKWKGKIVSQDPLGTGLGASLQHYYYHPELGPEFLKKL
                     FSEMQPTFGRDRRQITDWLAQGRFPLCLGCRDTNRAKQQGLPVDELDQVEWKEGFNVT
                     SGGGSISFIKGAPNPNAAKVFVNWFLSRRGQLALQKYPDLYGEDAPNSRRLDIPKDML
                     DSSMRMTPGRKYFDVSDPKYADMTPMFDLLKQIVKAKEAK"
     gene            complement(3520..4734)
                     /locus_tag="VMZ02_03140"
     CDS             complement(3520..4734)
                     /locus_tag="VMZ02_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70954.1"
                     /translation="MRSSTANIRHRGGFETRPLLSLFSIGNRARVFISLTILLLEMPA
                     FVGSTLAAESKALWQAEWDKTVAAAKKEGRLNFYVGRYGSEKLLNEFRKEFPEIKFVT
                     TNGAGNSLGTRIVAEARSGSMVADLFSGGAVTNFEILYKGKVLDSIKSALILPEVLDE
                     SKWYGGAHRYNDPEQQFVFIYIANPTSASLYFNTNLVNPKEFKSYWDLVQPKWKGKFV
                     SQEPTGTGIGPSLQFFYYNPDLGPEFLRKLFVEQQMVYSKDRRQMTDWLAQGKFSLCM
                     GCRDSERANKQGLPVDDMDMVDWKEGAHISSGGGSLGLIKGGPHPNAAKVFINWFLSR
                     RGQLALQKYEDLYGEDPPNSRRIDIPKDMLPAPSRLIEGRRYFDFSDAKHSDMTPIFQ
                     LVREFMKGREGK"
     gene            complement(4770..>5858)
                     /locus_tag="VMZ02_03145"
     CDS             complement(4770..>5858)
                     /locus_tag="VMZ02_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70955.1"
                     /translation="VQSSVYGADSKSAWQAEWDRTVRAAEQEGQVVVSIGGYGAIIDS
                     GVFQKTYPKIKITSITGAGTDLTTRITAERRAGKYLLDVYNGGGNSLFQVLYVGKMLD
                     PIKPALLLPEVLDATKWWEGKIKYADKDGQYIIVYEGNVAAGAGAGYNVQLVDPREYK
                     TYWDFLNPKLKGKIISTDIRKVRGAGIPWQFLYYHPELGPKYLRRLFGEMDVTLTADL
                     RQAVDWLGSGKFSLVLPIQGGQIYKAKNQGLPVEQFEPFHFKEGVNLSSAFGSLALMN
                     RAPHPNAAKVFINWLLSREGQTLFQKLISVPGDARNSRRLDVPKDHIAADEQRRDKMT
                     YFDTDDPDTKDLNPAMKLLDEILGAKKQ"
BASE COUNT         1290 a         1632 c         1612 g         1324 t
ORIGIN      
        1 tggcatggat gggctttgag gcgcgccacg ctgttctcga agtgctcgac aaggccattg
       61 ccgataaaaa agcccaggtc agagtgattg cttacgatct cagtgagccc gacgtcgtct
      121 ctcggctcga acggctcgga tcgcgcctaa aaatcctcat cgacgacagt gataagcatg
      181 gcaacgccgg ctcgggtgaa acccaatcgg cgcagcgcct tacgatctcg gccggcgcgg
      241 ccaatgtgaa gcgtcaacat atggggcagt tgcaacacaa caagaccatc gtagttgacg
      301 gggccaagtt caaagcggcg ttgtgcggct cgaccaatta cacctggcgc ggcttttttg
      361 tccaggcgaa caacgcaatt attttgcgcg gtaggaatgc gatcaaacca ttcgttgcag
      421 cattcgacga ctattggaat catcaaacgc cgggtaaatt tgccaacacc gcttcggcgg
      481 cctggacgag tttgggagtc gacggggtca ctgcgaaagt cgccttctcg ccgcactccg
      541 tggctaactc tctgctgcaa acgatcgccg acgatatcgc caccaagacc acatcgagct
      601 tgttctattc tttggccttt ttgtaccaga ctcccggagt catgttggat gcaatcaaaa
      661 aagtctccgc taacgacaag ttgtttgtct acggcatctc cgacagaaaa gtcggcggcc
      721 tcgacttgca aaagcccgac ggcaacgtgg cggcggtgtt tccgtctgcc cttaacacca
      781 acctgcccga accgttcaaa tccgagcctg tggggggcag cggcaatcgc atgcaccaca
      841 aattcctggt cgtcgatttc aacaaacccg gcgcgcgggt gtatatgggg tcgtacaact
      901 tttccggcac ggcggacacc aagaacggcg agaacctact gctgatccgc aaccgccgca
      961 tcgctgtgtc gtatatgatc gaagcattac gcctggtcga ccactaccat tttcgcgtgg
     1021 cacagcagga agcgcagcgc gccaggacca tgctgcgatt ggaaaaaccg ccgcgcctcc
     1081 caggcgaaac gccctggtgg tccaaatatt ataccaatcc gctaaagatt cgtgatcgag
     1141 agttgtttgg ctagcgccgg cctcgttgaa agagaatgcg cgtgaagctt tcggggagat
     1201 ttacatacgt cagaatttga cagcaagaat cggagtgcgg atcacccgta gcgttgttaa
     1261 tttagcgtta gaaagggagg aaggacaatg aatctttcga ataacaataa ccgaggaaaa
     1321 tctgtcaacc ggaccgactc gcgttggccg tttctcatgc gtcgcgaccg ggcggcggac
     1381 ggaaagttct tttattcggt gcaaaccacc ggggtctact gccgttcttc gtgcgcggcg
     1441 cggcgaccgc ggccggagaa cgtacagttc tatgcaactt gcgccgaggc cgagcaggca
     1501 ggatttcatc cgtgcaggcg ctgcaagcca aaccagccat ccgagagtgc aggccatgcg
     1561 gcaaagatta ccggggtctg tcggctcatc gaaacctctg aagagcttcc tagcctcgca
     1621 gtgatggcaa aacacgccgg tcttagtccg catcactttc atcgcgtttt tgcatcgctc
     1681 accggcgtca cgcccaaagc gtatggcgtc gcgcatcgcg caaagcggat gagagcggaa
     1741 ctgcgacgaa gcagttcggt taccgaagcg atctacggcg ccggctacaa ctccagcggt
     1801 agattctacg aatgctcgga caaggctctg ggaatgaaac caatgcaatt tcgcgccggc
     1861 ggcgctgaca ccaacatccg tttcgcggtc ggcgcgtgct cgctgggagc ggtcctcgtt
     1921 gcagcgagcg atcgcggcgt gtgtgcgatt tttttaggtg atgattcgga ggcgctcgtg
     1981 cgcgagttgc aagaccgttt cccgcgcgcc gagctcatcg gcggcaatgc cgagtttgaa
     2041 ctgttggttg ctaaagtcgt cggctacatc gacgatcccg cgctcggtat cgatctgccg
     2101 ctagacatcc gtggcaccgc gtttcaacag cgggtttggc aagcattgac aaaaataccg
     2161 gcgggctcga ccgcaactta catggaaatc gctcagcaaa tcggcatgcc caagtctgcg
     2221 cgcgctgtgg cgcgcgcctg cgcaaccaat ctgctagcga ttgcgatccc gtgtcaccgc
     2281 gtcgttcgta ccgacggcag cttgtccggt tatcgctggg gaatcgaacg caagcgcacg
     2341 ctaatcgaga aagagtccgg gagccaatcg cgcgtgccaa ggcacgcctg aattatttcg
     2401 cctctttcgc tttcactatc tgcttgagca gatcgaacat cggcgtcata tcggcgtact
     2461 tgggatcgga aacatcgaaa tacttccgcc cgggcgtcat gcgcatcgac gaatcgagca
     2521 tgtccttggg aatgtcgagg cgccgcgagt tgggcgcgtc ttcgccgtag agatccggat
     2581 atttttgcaa agcgagctgg ccgcgccggg aaagaaacca attcacgaaa accttggctg
     2641 cattggggtt gggcgcgccc ttgataaaac ttatcgaacc gccgcccgag gtgacattga
     2701 agccttcttt ccattcgact tggtccagct cgtcgaccgg caagccctgc tgcttggcgc
     2761 gattggtgtc gcggcaaccg aggcacagcg ggaaccgtcc ctgcgccagc cagtcggtga
     2821 tttgtcgtcg atcccggcca aaagtcggct gcatttcgct gaacaatttt ttgaggaact
     2881 ccggaccgag ctcgggatgg tagtagtagt gttggagaga tgcgcctagg ccggtgccca
     2941 acgggtcttg agaaacgatc ttgcctttcc attttggcgc caccaaatcc cagtaggatt
     3001 tgaactcttt ggggtttacc agggttgtat tgtaataaaa tcccgaggat gtcggattgg
     3061 cgatataaac caacacatga cgctgctcag gatcggtaaa acgaattcgt ccctcatacc
     3121 acttgctctc gtcgagcacc tccggcaaga ccagaagaga cttgatcgag tccagcgcct
     3181 taccctcgta aaggacttca tagtttgtat tcgccccgcc gctgaacaag tctgcgacca
     3241 catggccggc gcggatctcg gtaacgattc gagttcccag tgagtttccc gcgccgttga
     3301 cggtaacgat tttaatctcg ggaaactctt tgcggaattc gttgagcagc ggctcgctgc
     3361 catagcggcc gacgtaaaaa ttgagtcttc cctccttttt tgctgcggcg atagtctgct
     3421 cccaactcga ttgccatgcg ggctttacct cggcgccgaa tcctaccgga gttgaagtaa
     3481 aaacgcagag aacaaacaga gcgaatcgga tatgcatgat cattttcctt cacgtccctt
     3541 cataaattcc cggactaatt gaaagatcgg tgtcatatcg gagtgcttcg cgtcggagaa
     3601 gtcgaagtag cgccgtcctt cgatcagccg gctgggtgcc ggcaacatgt cctttgggat
     3661 gtcgatgcgc cgcgagttgg gcgggtcttc accataaagg tcttcatatt tctgcagggc
     3721 taattggccg cgccgagaca ggaaccagtt gatgaaaacc ttggccgcgt tcggatgagg
     3781 accacccttg atcaaaccca aagaaccgcc gcccgacgag atatgcgcgc cttccttcca
     3841 atcgaccatg tccatgtcgt ccaccggcaa gccctgcttg ttggctctct cggaatcgcg
     3901 acagcccatg cataaggaaa actttccttg cgccagccag tcggtcattt gccggcggtc
     3961 tttcgaatag accatctgct gctcgacgaa taatttgcgg agaaattccg ggccgagatc
     4021 gggattgtaa tagaaaaact gcagcgacgg tccgatgccg gtgccggtcg gctcctgcga
     4081 gacgaattta cctttccatt tcggttggac caagtcccag tacgacttga attcttttgg
     4141 attgaccaag ttggtattga aataaagact ggcagacgtc ggattggcga tgtagataaa
     4201 aacgaattgc tgttccggat cgttgtaacg gtgcgcgcca ccgtaccact tggactcgtc
     4261 gagcacttcc ggcaggatta aagccgactt gatggaatca agcactttgc ccttgtagag
     4321 tatctcgaag ttcgtcacgg ccccgccgct gaacagatcg gcgaccatgc tgccggagcg
     4381 cgcctccgcg acgatgcgcg tgcccagcga attgccggcg ccgttggtag tcacaaattt
     4441 aatctcggga aactccttac gaaattcatt gagcaacttc tcgctaccgt agcggccgac
     4501 ataaaagttc agccgtcctt ctttttttgc cgcggccaca gtcttgtccc actctgcctg
     4561 ccagagcgct ttgctctcgg cggctaaagt tgatccgacg aaggccggca tttccaacag
     4621 caaaatcgta agggatataa aaacgcgcgc ccggttgccg atgctgaata atgagagcag
     4681 agggcgggtt tcaaacccgc ccctatgtcg gatgttcgct gtggaacttc tcatgcccgt
     4741 agcgtatttg cggccattta gtttaattct tactgcttct tagcgccgag gatttcatcc
     4801 aacagtttca tcgccggatt caaatctttg gtgtcgggat cgtcggtatc gaaataggtc
     4861 attttgtcgc gccgctgttc atccgcggcg atatgatcct ttggcacgtc caggcgccgt
     4921 gagttgcgcg catcgccggg cacggagatt aatttttgga acaaggtctg gccttcgcgc
     4981 gacaacagcc agttaataaa taccttggca gcattgggat gcggcgcgcg gttcatcaac
     5041 gctaaggacc cgaatgctga cgataggttc acgccttctt tgaaatggaa tggctcaaac
     5101 tgctccacgg gcagcccctg atttttggct ttgtaaatct gcccgccctg gatcggcaga
     5161 actaagctaa attttcccga gccgagccag tccaccgcct ggcgcaggtc ggccgtcagc
     5221 gtcacatcca tttcgccgaa caagcggcgt aaatatttgg gtccgagctc gggatgataa
     5281 tagagaaact gccagggaat ccccgcgccg cgcactttgc ggatatcggt cgagataatt
     5341 ttccctttga gtttcggatt gagaaaatcc cagtaggtct tgtattcccg cggatcgact
     5401 agctggacgt tgtagccggc gccggcaccg gccgcaacgt tgccttcata gacaataata
     5461 tattggccgt ctttatcggc atattttatc ttgccttccc accacttggt cgcatccagc
     5521 acttcgggca gaagcaaggc tggcttgatc gggtcgagca tcttgccgac atagagtact
     5581 tgaaaaagcg aattgccgcc gccgttgtaa acatcgagga gatatttgcc ggcgcgtcgc
     5641 tcggcggtga tgcgcgtcgt taggtcggtg cctgcaccag tgatgctggt aatcttgatc
     5701 ttaggatagg ttttttgaaa cactccggaa tcgatgatcg cgccgtaacc gccaatggaa
     5761 accacgactt gtccttcttg ttcggccgcg cgcacggtgc gatcccattc cgcttgccaa
     5821 gccgacttcg aatcggcgcc gtaaaccgaa gactgaac
//
LOCUS       DATLCH010000107         7523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_337994, whole genome shotgun sequence.
ACCESSION   DATLCH010000107 DATLCH010000000
VERSION     DATLCH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337994"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VMZ02_03150"
     CDS             <1..430
                     /locus_tag="VMZ02_03150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HUR70956.1"
                     /translation="GVTVITTRDKDGAPQGLTANAFMSLSLNPPLIIVSVDKAATCYA
                     CFEAGNGFCVNILSEDQEDVSRRFATKGIDKFADLKWHTGGNGVALIDGALGQVECKI
                     SACYEGGDHTIVVGEILNVAANGERPLIFFKGKYQKLPLV"
     gene            complement(462..1433)
                     /locus_tag="VMZ02_03155"
     CDS             complement(462..1433)
                     /locus_tag="VMZ02_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70957.1"
                     /translation="MAANEKNALHLATHSGWYRYERRGEEWEQTDRALTFWQMSCIQV
                     DPDDPQHVYAGTEHSGMFVSKNGGKDWSRAEPNVPVLEMSAMLALPGKVLVGTRPAAL
                     YVQKNGGSWQEVPGIRQGAFGGTFPPNPDLAPRTRVLTTEKASGNRVYAGIEVGGILV
                     SEDDGETWVKRNDGLTDPDLHQILSASKKPGVLVAACGEGVARSIDRGNSWQPVTPAG
                     GRTYGNALAEDDQGNIYLGITQDRPRTWIRGGRAKTAIFKSSDGANWEFLTDQMSGGV
                     MEMCANAEGNGVMVATAEGEVLNVDSNGKTRTLVNGVPCINALVLGA"
     gene            complement(1638..2444)
                     /locus_tag="VMZ02_03160"
     CDS             complement(1638..2444)
                     /locus_tag="VMZ02_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HUR70958.1"
                     /translation="MPFPDNLLVGSSSWSCSDWLGSFYPANLKPGQFIEAYARKFRAV
                     EIDSTYYSIPPPHVVASWREQTPPGFVFAAKIPGAITHEKVMRNCQNELTAFLNNISI
                     LGDRLGPLLLQFPYFTRETFPSRQPFDNLLKPFLQCLPKEFKFALEIRNKNWLSWDFL
                     EMLRDHQVAFALVAQAWMPGIDTLAKALDLVTSDFAYVRFIGDRKAIESKTKRWDRMV
                     EDKADEMKVWISELKKITDRGVKTYAFFNNHYAGFAPGSIKQFNDLWPQS"
     gene            3152..3658
                     /locus_tag="VMZ02_03165"
     CDS             3152..3658
                     /locus_tag="VMZ02_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUR70959.1"
                     /translation="MFPFHPRPLMDTYDSKTASRIVGVSLRQLQYWDEQKFLRPSIKL
                     AGGRGTKRLYSFHDLVCLKVVKDLARYGFSLKKIRRCLRPLKEQTDPTTRPPPSLRYL
                     TDGEELFVITSDRQKILHAMERQFVLSLGIGSLVRELDGAVKRFGDVGGKPNRATRQL
                     DVGRRELA"
     gene            3664..6237
                     /gene="mutS"
                     /locus_tag="VMZ02_03170"
     CDS             3664..6237
                     /gene="mutS"
                     /locus_tag="VMZ02_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HUR70960.1"
                     /translation="MDGTKVTPMLQQYLQIKERYQDAILFFRLGDFYEMFFEDAHVAS
                     KVLDITLTSRNKSDEGSVPLCGVPYHSAQPYIQKLLEAGHKVAVCEQVEDPALAKGVV
                     KREVVRVITPGTVTADEALDGRGNNFLAAVCQGESSYGLAVSDITTGEFRCSEIADEQ
                     AFLDELGRVQPSEIVVAARSSRLRERIYKEYPTIHMTSVDDETFADATEAGPNHAAGI
                     SAEGARAAAAIINYLTANIPESVKLLRDLQPYVVSNFLILDEITRVNLELLATAQGDR
                     RGSLLAILDRTQTPIGARRMRQWLLYPLLDEMEIRARYDAVQELAEGFALREDLKLRL
                     EKIQDLERLSGRVLSGSATPKDLIAIKQTLEAASSVRQLASQFTAPMLVGLCENLQEL
                     PELVALIGRAIIDDPPFAFKEGGFIRPGFDGELDEIRGMRSHAKDWIAQFEAGERKRS
                     GITSLKVRYNRVFGYFIEVTNSHLKSVPADYIRKQTLANGERYFTPELKEYEAKVLNS
                     ESLMEKLEANLLTRVREQAALHYPALKVMSNALAILDVLAALADVAETQRFVRPRVDN
                     STIFNVREGRHPVVENAVGRGAFVPNDCKIDAESEQIILLTGPNMAGKSTYMRQNALI
                     VILAQMGGFVPAAEARIGIVDRIFTRIGAADSLARGESTFMVEMKETANILHHASRRS
                     LILLDEVGRGTSTFDGISIAWAVAESLHENCERPRTLFATHYHELTELALNHERIKNF
                     NFAVKEWRGEIIFLRNLVAGAASHSYGIHVARLAGMPAPVIERAKLILARLEDSQVAG
                     PSVTGKRRVEESNEMPVQMGLFNVTDDRLRNRLNQLDIANLTPIQALNVLHELSEEAK
                     K"
     gene            6282..>7523
                     /locus_tag="VMZ02_03175"
     CDS             6282..>7523
                     /locus_tag="VMZ02_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HUR70961.1"
                     /translation="MKFVAMAAVTGLCLWPEGKLSGQVRNEPPPREPMPGYAQANAAK
                     SSDNQTPAPLARARQKYAPIAAAPSGPSTQSSGKVQVTGLRFLSAKNFTRVMLDLSQD
                     AKYQLRRLQEDPSKGVPPRIYIDISNARLAMPSKEQLPAEDRLLRQVRVGQYGADVVR
                     VVLDLTSVRDHKAFILPEPYRLVIELQGQKMPDAAPIMEEPAKVETSPRVSTPINEKK
                     SSQALGSSSGIRKIVIDPGHGGKDPGAIGVGGLMEKDLVLSVAKKLAAKLTAEMGVQV
                     VLTRRDDRFIALEDRTYLANAEDADLFISLHMNASPNPEAHGVETYYLDNTTDEAAMR
                     LAARENASARKNISDLQFILSDMTQNMKLEDSITLAHRLQQSVVAGMSKEIGEVRDLG
                     VKKALFYVLVGARMPSVLVEMF"
BASE COUNT         1683 a         2036 c         2152 g         1652 t
ORIGIN      
        1 cggcgtgacc gtgatcacca cccgggataa agacggtgcg ccgcagggct tgacggccaa
       61 tgctttcatg tcactgtcgc tcaatccgcc gctcattata gtcagcgtcg acaaggcagc
      121 cacatgctac gcctgcttcg aggcgggaaa cggtttttgt gtcaatattt tgagtgaaga
      181 tcaggaagat gtttcgcgcc gctttgcgac caaggggatc gataaatttg ccgatttaaa
      241 atggcatacg ggcggcaatg gtgttgccct catcgacggt gctctaggtc aagtggagtg
      301 caaaatctcc gcttgctatg aaggcggcga tcacacgatt gtcgttggcg agatcctcaa
      361 cgtcgccgcc aatggcgagc ggccgttgat ttttttcaaa ggcaagtatc agaagttacc
      421 gttggtgtaa aaaggggcga ccggcagtcg ccccgacgag attatgctcc taataccagt
      481 gcgttgatgc acgggacgcc gttaaccaat gtccgtgtct tgccattcga atcgacattc
      541 aacacttctc cctcggcggt ggcgaccatg acgccattac cttccgcgtt ggcgcacatt
      601 tccatgactc cgccgctcat ttgatcggtc agaaactccc agtttgcgcc gtccgaggat
      661 ttgaagatcg ccgtcttggc gcgcccgccg cgtatccagg tgcgcggccg gtcttgagtg
      721 atgccgaggt aaatgttgcc ttgatcatct tcggcgagtg cgttgccgta agtccgcccg
      781 ccggcggggg ttacgggttg ccagctattg ccgcgatcga tgctgcgcgc gacaccttcg
      841 ccgcatgcag ccaccaatac gcctggtttt ttgctagcgg ataaaatctg gtgcaggtca
      901 ggatcagtga ggccgtcgtt gcgcttgacc caagtctcac catcgtcctc gctgaccaaa
      961 attccaccga cctcgatgcc ggcgtaaacg cggttgccgg aagctttctc cgttgtcagc
     1021 acccgcgtgc gcggtgccaa atcgggatta ggtggaaagg tgccgccgaa ggcaccttgg
     1081 cgaatgccag ggacttcctg ccagctgccg ccgttttttt gcacatagag ggcggccggg
     1141 cgagtgccga ccaggacttt tcccggcaag gcgagcatcg ccgacatttc cagcaccggg
     1201 acgttgggtt cggcgcgact ccaatctttg ccgccgttct tactaacgaa catgccggag
     1261 tgttccgtgc cggcatagac atgctgaggg tcatcgggat ccacttggat gcagctcatt
     1321 tgccagaagg tgagcgcgcg gtcggtctgc tcccactcct ctccgcggcg ctcataacga
     1381 taccagccgg aatgggttgc gaggtgaagg gcattttttt cattggcagc catagggtcc
     1441 tccaaaagat gtgcttcgat tgacttcgac acctcgacca tacgcccgcc ggtgaatttg
     1501 cgtcaagtct tgaagcatca aggtcgtggt tctatggaat ctgttccaca aataaaaaga
     1561 tttctcgcgt tgctcgaaat gacaatccaa ttgccgtgtc gaggaagaat gagaacagcc
     1621 taagcggtag aaagcaatta gctctgcggc caaagatcgt tgaattgctt gatcgatccg
     1681 ggtgcgaagc cggcgtagtg gttgttgaag aaggcatagg tcttcacgcc tctgtcagta
     1741 atcttcttga gctcgctgat ccaaaccttc atttcgtcgg ccttgtcttc gaccattctg
     1801 tcccagcgtt tggttttgct ctcgatggcc ttgcgatcgc cgatgaagcg cacgtaggca
     1861 aaatcgctgg tgaccaggtc aagcgctttg gctaacgtgt cgatgccggg catccacgct
     1921 tgcgcaacca aggcgaatgc tacttggtgg tcgcgcagca tttccaggaa atcccagctg
     1981 agccaattct tgttgcgaat ctccaaagcg aacttgaact ctttcggcag gcattgcaga
     2041 aatggcttga gcaagttgtc gaacggttgg cgtgacggaa aggtctcgcg agtgaaataa
     2101 ggaaactgca acaacagggg acccaaacgg tcccccaaga tcgagatgtt atttaaaaat
     2161 gctgttaatt cgttttggca atttctcatt accttttcat gcgtaatcgc gccgggtatt
     2221 ttcgcggcaa aaacaaatcc cggtggagtc tgttcgcgcc aactcgccac cacgtggggc
     2281 ggcggaatgc tgtagtaagt agaatcgatc tccaccgcgc gaaatttgcg agcgtaagct
     2341 tcgatgaatt gtccgggttt gagattggcg gggtaaaagg agccgagcca gtcgctgcat
     2401 gaccagctcg aactgccgac gagtaaattg tcaggaaatg gcatcgctac ttttcaccac
     2461 atcggcggcg cgcgatcaat agcagttccg acggttgcag tgaacataag agtcgcttat
     2521 caccatgaag tattcatcag cttacgctcg tcagcagaga atgaattgca gggaagccgc
     2581 tccaaatcgt tcctttccat gaatcgtcat gccagcgcag gccgacattt atttcgttgt
     2641 cgtcgcacca cccggcctgg ataccggcct ccgccggtat gacggaatgt tcgtgccaat
     2701 tcgtccccgg gactgtgcat ctttatttcg aaggagtatg gagcatacag gtgtctgcaa
     2761 aatgccaaat gtaccttcca cacgtatcat cctttcaagc gtcatgccgg cgtgccgcgc
     2821 atccggggtg tgaggggatg gataccggct tgcgccggta tgacggaagc ttcttgcccc
     2881 ttcatcgcca ggatcgtacc tgcgcagcat ggagccatca ctgcgaacat tgatgcctga
     2941 tcaaaaacct ccgcgactcg gactcgaaca agtcgcaata ctgccaagcc ggattggtcg
     3001 tcatttcgac tagaaggaga aatctttccg cctgcacgaa ttcgcaaata gcgactgtcg
     3061 tccgacagaa tcaaccatcg aaattgcggt atgaaccggc aacttttgat tgacttcacg
     3121 gtaccttgcg tttactttca tgaaaacatt gatgtttcca ttccacccgc gaccgctcat
     3181 ggatacctac gatagcaaaa ccgccagccg cattgtcggg gtgagtttgc gccagctaca
     3241 gtattgggac gagcagaagt ttttacgacc gtcgatcaag ctcgccggtg gacgcggcac
     3301 caagagactt tattcatttc acgatctggt ctgtctgaag gtggtgaaag atctggcgcg
     3361 ttatgggttt agtctcaaga aaattcgccg ctgcttgcgg ccgctcaaag aacagaccga
     3421 tccaacgacg cgaccaccgc cgtcgctgcg ttatctgacc gacggcgaag agctgttcgt
     3481 catcaccagc gatcggcaga agattctcca cgccatggag cggcagttcg tgctttcgct
     3541 cggcatcggc agtttggtgc gcgaactcga cggcgcggtc aaacgcttcg gggacgtcgg
     3601 cggaaaaccc aacagggcaa cgcggcaact ggacgtgggg cgccgcgaat tggcgtaatc
     3661 atcatggacg gaaccaaggt tacgccgatg ttgcagcagt acctccaaat caaggagcgg
     3721 taccaagacg cgattttgtt ttttcgcctc ggcgattttt acgaaatgtt ttttgaggac
     3781 gcccatgtcg catcgaaggt tctcgacatt actctgacct cgcgcaataa gagtgacgaa
     3841 ggttcggtgc cgctgtgcgg cgtgccgtat cattccgcgc agccgtatat tcaaaaactc
     3901 ctggaagcgg gacataaagt tgcggtatgc gagcaagtgg aggatccagc cctagccaag
     3961 ggtgtggtca agcgcgaagt cgtgcgggtg attacgccgg gcactgtgac ggctgatgag
     4021 gcgctcgacg gccgcggcaa taactttctc gccgcggtat gccaagggga atcatcctac
     4081 ggtttggcgg tcagcgatat taccaccggc gagtttcgct gtagtgaaat tgccgacgag
     4141 caggcgtttc tcgacgagct tggccgcgtc cagccgagcg aaatcgtggt ggcagcgcgc
     4201 agtagccgct tacgtgagcg catttataaa gaatacccaa ccattcatat gacctcggtg
     4261 gacgatgaaa ctttcgccga tgcaactgaa gccgggccaa accatgcggc gggtatttcc
     4321 gcggaggggg cgcgagcggc ggcagcgatt attaattacc tcacggccaa tattcccgag
     4381 tcggttaagc tcttgcgcga tttgcagccg tatgtggtct cgaatttttt aattctcgac
     4441 gaaatcaccc gggttaattt agaattgctc gccacggcgc aaggcgaccg gcgcggttcg
     4501 ctattggcga tactcgatcg cacgcaaacc cccataggcg cgcgccggat gcgccagtgg
     4561 ttgctttatc cgttgctcga cgagatggag atccgcgccc gctacgatgc cgtgcaggag
     4621 ctcgccgaag ggttcgcgct gcgcgaagac ctcaaacttc gcttggaaaa aattcaagac
     4681 ctagaacggc tcagcggccg ggtcttgtcg ggcagcgcga caccgaagga tttgatcgcc
     4741 atcaaacaaa ctcttgaagc cgccagcagt gtgcgtcagc tagcctccca atttacagcg
     4801 cccatgctag tcggcctttg cgagaatctg caggaattgc cggaattggt ggcgctaatc
     4861 gggcgggcga tcatcgatga tccaccgttt gcgttcaagg aaggcggttt catccgcccg
     4921 ggctttgatg gcgaactcga cgagatccgc ggcatgcgca gccatgccaa ggattggata
     4981 gcccaatttg aggctggcga gcgtaagcga agtggcatca cgtctttgaa agtgcgctac
     5041 aatcgggtgt tcggctactt catcgaagtg accaacagcc atttaaaatc cgttccggcg
     5101 gattacattc gcaaacagac attggccaat ggcgagcgct atttcactcc ggaactgaag
     5161 gaatacgaag ccaaggtgct caattccgaa agcctcatgg aaaaactcga ggcgaatctg
     5221 ctgacccggg tgcgcgagca agcggcgctg cattatccgg cgctgaaggt gatgagcaat
     5281 gccttggcta ttttggatgt gctcgcggcc ctggccgacg tcgccgaaac tcagcgcttc
     5341 gtccgtccgc gtgtcgataa cagcaccatc ttcaatgtcc gggaaggacg ccatcctgtg
     5401 gttgagaacg ccgtggggcg cggcgctttt gtgcccaacg attgcaagat cgatgcagag
     5461 tccgagcaga ttatcctgct tactggacca aacatggcgg gtaagtcgac ttatatgcga
     5521 cagaacgcgc tgattgtcat cctcgcccag atgggcggct tcgtgcccgc agccgaagcg
     5581 cggatcggca tcgtcgaccg gatcttcacg cgcattggcg cggcagactc gctggcccgc
     5641 ggcgagtcga ccttcatggt tgaaatgaag gagacggcaa acatcctgca ccatgcctcg
     5701 cggcgcagct tgatcctgct cgacgaggtg gggcgaggca cgagcacctt cgatggtatt
     5761 tccatcgcct gggcagtcgc agaatcactg catgaaaatt gcgagcggcc gcgcacgttg
     5821 ttcgcgacgc actatcatga gctgaccgaa ttggcgctca accacgagcg tattaaaaac
     5881 ttcaacttcg ctgtcaagga gtggcgcggt gaaattattt ttctgcgcaa tctggtcgcc
     5941 ggggcggcga gccatagtta cggtatccat gtggcgcgtc tcgccggcat gccggcgccg
     6001 gtgattgaac gggccaaact catactggct cgattggaag atagccaagt agccggtcca
     6061 agcgtcacgg gcaagcgccg cgtcgaggaa agtaacgaaa tgccggtgca gatgggtttg
     6121 ttcaatgtaa ccgacgatcg cctgcgcaat cggttaaacc aactcgacat cgccaatctg
     6181 acgccaattc aagccttgaa cgttctccat gagttaagcg aggaagccaa aaagtgacga
     6241 cccgcttgat gggagtagca atgatgcgaa agcgtggttt tgtgaaattt gtcgcaatgg
     6301 ccgcagtgac ggggctttgc ctctggccag agggcaagct gtcgggccag gtgcgcaacg
     6361 agccgccgcc acgagaacca atgcccggct acgcgcaagc gaacgccgca aagtccagtg
     6421 acaaccagac accggcgcca ttggcaaggg ctcgtcagaa atacgcgccc attgcagcgg
     6481 cgccaagcgg accatcgacg cagtcgtcgg gtaaggttca agttaccggc ttgcgctttt
     6541 tgtcggcgaa aaattttacc cgggtaatgt tggatctatc gcaggatgcc aaataccaac
     6601 tacgccggct acaggaggat ccgtccaagg gcgttccgcc gcgtatttat atcgacatct
     6661 ccaatgcgcg cttggcgatg ccttcaaaag aacaacttcc ggcggaagac cggctcttgc
     6721 gccaagtccg cgtcggtcaa tatggcgccg atgtggtgcg tgtagtcttg gatttgacca
     6781 gtgtgcgcga tcacaaagcg tttattctgc ctgaaccgta tcgcttagtg atcgaattgc
     6841 agggacagaa gatgcccgac gctgctccca ttatggagga gccggcgaag gtcgagacga
     6901 gtccgcgagt cagcacgccg atcaacgaga aaaaatcgtc acaggctttg ggcagctcca
     6961 gcggcattag gaaaattgtc atcgatccag ggcatggcgg caaagatccc ggggcgatcg
     7021 gtgtcggcgg cttaatggaa aaagatctgg tactgagtgt cgccaagaag ctcgccgcga
     7081 aactcacggc tgaaatgggc gttcaggtag tcttgacccg cagggacgat cgttttattg
     7141 cccttgaaga tcgcacctat ctcgccaatg ccgaagatgc cgacctgttt atttcactgc
     7201 acatgaatgc gagccctaac cccgaggccc acggcgtcga aacttactat ctcgataaca
     7261 ccaccgacga agcggcgatg cgattggcgg cccgggaaaa cgccagcgcg cgcaaaaaca
     7321 tttcggatct acaatttatc ttgagcgata tgacacagaa tatgaagctc gaggattcga
     7381 ttactctggc gcatcgcctg cagcagtcgg tcgtcgcagg catgagcaaa gagatcggcg
     7441 aggtgcgcga tcttggcgtc aaaaaagcgc ttttttatgt tctcgtcggt gcgcgaatgc
     7501 ccagcgtgtt ggtcgaaatg ttt
//
LOCUS       DATLCH010000108         7248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4809806, whole genome shotgun sequence.
ACCESSION   DATLCH010000108 DATLCH010000000
VERSION     DATLCH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4809806"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1280
                     /gene="purM"
                     /locus_tag="VMZ02_03180"
     CDS             237..1280
                     /gene="purM"
                     /locus_tag="VMZ02_03180"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HUR70962.1"
                     /translation="MAPPKGLTYAQAGVSIEAGDALVRRIAPIAKQTKVPGVLAGVGG
                     FSALFDLKSRGYRAPVLVSSTDGVGTKLKIAFQTGIHDTIGIDLVAMGVNDILTQGAA
                     PLFFLDYFVCGKLDVKITEAVVRGIAAGCRQAGCALIGGETAEHPDDFPRGEYDLAGF
                     VVGVVEKNQIIDSAAIVAGDVLIGIPSSGLHSNGYSLARKILLERGGLKLHQRVPELG
                     RKLAEELLEPTRIYAKITKKLFGEFRIKGAAHITGGGITGNLPRVLPRGRHAVIERSS
                     WPVPPVFSLLQKIGAVAQPEMDATFNMGLGMIFVAAKKDADRVVSALKRMGEACFVIG
                     AIRKGARGATIRS"
     gene            1285..1947
                     /gene="purN"
                     /locus_tag="VMZ02_03185"
     CDS             1285..1947
                     /gene="purN"
                     /locus_tag="VMZ02_03185"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HUR70963.1"
                     /translation="MTKQLPLAVLISGGGTNLQAIIDAIETRALDAKIQFVLSNKADA
                     FGLVRAQKHGIHTEVLDHKAYPSREAYDHAVVDLLHRRGVELVVLAGFMRLLSPVFIK
                     AYSNRIMNIHPALLPSFPGLHVQKKAVEHGVRFSGCTVHFVNEECDEGPIIIQAVVPV
                     FPDDTDETLAARILRQEHRIYPRAIQLYAEGRLHVNGRRVLVDGLNKDEGQVLIQPPL
                     ER"
     gene            1944..2918
                     /locus_tag="VMZ02_03190"
     CDS             1944..2918
                     /locus_tag="VMZ02_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HUR70964.1"
                     /translation="MKKTKPLVILLVKVLVSGGLLAFFFSRVHPERFLHTFATANYSW
                     IALALIIYLFTQSISAVRWAALARPLGIVTPFTDLFKYYLIGMFFNLFAPGTVGGDVT
                     RIFYLVRDEEAHAKGRAVTTTHAAISVLMDRAIGMIVLVWLGAAGLLLFPEYAVPPMV
                     RSITLLLAAGFVGALLLLPLVRRVLPEGGHPIVVKIRLALRSYRTQWRALAVAALLSL
                     IVHLIQAWMHRIMGRALGLDVPYSFCLIVYPLVGTFSAIPVSLNGLGLREGGYIFMLA
                     VIGIGSEQGIAFGLLLFLIVALDSLIGGVLFLLQKSPKAGGVIAEKAT"
     gene            complement(2915..4267)
                     /gene="mgtE"
                     /locus_tag="VMZ02_03195"
     CDS             complement(2915..4267)
                     /gene="mgtE"
                     /locus_tag="VMZ02_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HUR70965.1"
                     /translation="MKPAPIDYDTVREMLRTATPDRVLRFIGKSHPADIALLFKGLDS
                     AEVRQLFDVLFSSRRAAKTLKELPPELLPDILALIDDEKVGRLIVRADPDDAVTFIDS
                     LPEDRRERVLALLDPERRAAVREIINYPDGTVGRLMTTDFMALPPDATAQGAIDKIRE
                     RGELESFFYLYVVEKDGKLVGVVPIRNLVIAPPMRKLRDMMIPDPIKADVFMDQEDAA
                     RIVSKYELLALPIVDEGGRLEGIITVDDVIDIIKEESTEDMYKMVGLAEEDRVFTPVS
                     RSVRMRLPWTILNLLTASLAASIVGLFEGTLHEIVALVTFMPVVAGVGGNGATQTATV
                     IIRAIALGELEFSSAWKAIVKQVSVNICIAVAAGAMIAVAAGLWKGNPYLGLILAGAM
                     LLNLGLMAGFAGAVIPLLLKALKLDPALGSGIIVTGLTDAFGFLSFLGLATMFRSYLI
                     "
     gene            4410..5843
                     /locus_tag="VMZ02_03200"
     CDS             4410..5843
                     /locus_tag="VMZ02_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUR70966.1"
                     /translation="MLGQNQSTARRCMAASLTAYLGWLAIQTSGATHAANLALDGSRP
                     PSFASIAKKTMPAVVNISTLTQRSPRSGSNDPIEDFFNRFFGESQPRENNSRSLGSGI
                     LISRDGEILTSYHVVRSADTIKVKLSDQTEHEARLIGKDDRTDLALIKIRRSGGNLPF
                     AKLGNSNQLEVGDWVMAIGNPFGLEHTVTAGIVSAKGRVIGAGPYDNFIQTDASINPG
                     NSGGPLISAEGEVIGVNTAIFSQSGGNVGIGFAIPIDLAKKVLDQLRKNGKVVRGWLG
                     VRAQDLSATQVAALGTIRSAAELAIVTEVTDKSPAAEAGIKTGDVIVEFNGKPVPRSS
                     EFPSVIADIPPGQKVSLKILREKKEQTIAVRIGELADDADLNQQVEARDAELGLRVQR
                     ITPEAARRLGLSSPKGVLVVEVQPGSPADLVGIEPADVIREVNQRAVTNVKEFERATR
                     QGRRGDRILLLVQRGDNAVFFAVKRKG"
     gene            5852..6040
                     /locus_tag="VMZ02_03205"
     CDS             5852..6040
                     /locus_tag="VMZ02_03205"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HUR70967.1"
                     /translation="MFGLGVPELMVILVIALVIFGPSKLPQIGSGLGKAIRDFKKGVT
                     EKDDEETNVKKDGQDLPR"
     gene            complement(6219..6869)
                     /locus_tag="VMZ02_03210"
     CDS             complement(6219..6869)
                     /locus_tag="VMZ02_03210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR70968.1"
                     /translation="MAEIEAHESKLYGEFAPLYDKIFGKIFYNRIERVIENLDIPIGA
                     KILEIGAGTGTSFPAYPHHCDVTGIDLAPDMLSRARHKIHENGWHHFKVMEMDALNLT
                     FPDNSFDYVMAFHVVTVVPDPVRMIAEAKRVCKPSGRIVILNHFTTDMPVIGSITQAI
                     DPLTRLLGWRTDLKLKPFIASTGLMVEKVYKLNKTSLYTVVLGRKSAPGFRTTREL"
     gene            complement(6899..7093)
                     /locus_tag="VMZ02_03215"
     CDS             complement(6899..7093)
                     /locus_tag="VMZ02_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR70969.1"
                     /translation="MVEREEELLNSKQVAVILDMSPDTVNEFARKNILPAFKKGKQWR
                     FRKRDISIFYKKQPKDIRDA"
BASE COUNT         1555 a         1948 c         2088 g         1657 t
ORIGIN      
        1 tattggagac catcgccatc gatttaccgc ggccgcgaac catggcaatg atgagcgcgg
       61 taaaattcgg cgaatacacc cttaggattc gtgctttgct ggcgtcggct ggcggcgcca
      121 atgccgcggc aatcggctcg actatgtgaa attccgcctt cagtgcgcta tgatactgac
      181 gatgaggcag ggcagtacgc cgctccccaa tactccgaaa ataaatttag gtttgcatgg
      241 cacctccaaa aggactcacc tacgcccagg ctggcgtcag catcgaagcc ggcgatgcat
      301 tggtgcggcg catcgccccc attgccaagc aaaccaaggt gcccggagtg cttgccggcg
      361 ttggcggttt cagcgcgctg ttcgatctta aatcgcgcgg ttaccgtgct cccgtgctgg
      421 tgtcgtcgac tgatggcgtc ggtaccaagc taaagatagc ttttcagacc ggtattcacg
      481 acactatcgg catcgatcta gtcgccatgg gcgtcaacga tatcttgacc cagggcgccg
      541 cgccgttgtt tttcctggac tactttgttt gtggcaagct cgacgtaaaa attaccgaag
      601 cggtggtgcg cggcatcgcg gctggatgtc gccaagctgg ctgtgcgctg atcggcggtg
      661 agaccgcgga acatcccgac gattttcccc gaggcgagta cgatcttgcc ggcttcgtcg
      721 tcggtgtcgt ggagaaaaac cagattatcg attccgccgc gatcgttgcc ggcgacgttt
      781 tgatcggtat cccttccagt ggactgcata gcaacggcta ttccttggcg cgcaagatct
      841 tgctcgaaag gggcggatta aaattgcacc aacgtgttcc cgagctcggt cgtaaactcg
      901 cggaagagtt gctcgaaccg acgcggattt atgcaaaaat caccaagaaa ttgttcggcg
      961 aatttcgcat caaaggcgcg gcgcatatca ccggcggcgg tattacggga aatctaccga
     1021 gagtgttgcc gcgtggacgg cacgcggtaa tcgaacggtc gagttggccg gtgccgccgg
     1081 ttttcagtct gcttcaaaaa atcggcgcag tcgcccagcc ggaaatggac gcgactttta
     1141 acatggggct cggcatgatt ttcgttgccg ccaagaaaga cgccgatcgg gtcgtcagcg
     1201 cgctgaagcg catgggcgag gcttgctttg ttatcggcgc aattcgcaaa ggcgcccgcg
     1261 gcgcgacgat tcgttcttaa aattatgaca aagcaacttc cgttagcggt gttgatttcg
     1321 ggcggcggaa ctaatctgca ggcgatcatc gacgcaatcg agacccgggc gctcgatgct
     1381 aagatacaat ttgtcctgag taacaaagcc gacgcgttcg gcttggtccg ggcgcaaaaa
     1441 cacggcatcc ataccgaggt cttggaccat aaggcttatc cgagccgcga ggcgtacgac
     1501 catgcggtgg ttgatctgtt gcaccggcgc ggtgtcgaac tggtggttct cgccggcttt
     1561 atgcgcttgc tttcgccggt tttcataaaa gcctactcca accgcataat gaatatacac
     1621 ccagccttgc tgccgtcatt tccgggcctc cacgtgcaga aaaaagccgt cgaacacggc
     1681 gtacgctttt ccggttgtac agttcatttt gtcaacgagg agtgcgacga gggacccatc
     1741 atcatccagg cggtggtgcc ggtgtttccc gatgacaccg acgaaacctt agcggcacgg
     1801 attctcaggc aggaacatcg gatttatccc cgggcgattc agctctatgc cgagggtcgc
     1861 ctgcatgtaa atggtcggcg ggtgttggtc gatggcctga ataaggatga aggacaggtg
     1921 cttattcaac cgccgcttga acgatgaaaa aaaccaaacc gcttgtcata ttattagtaa
     1981 aggtcctcgt tagcggcggt cttctggctt tcttcttctc gcgagttcat cccgagcgtt
     2041 ttctacatac ttttgcgacg gcgaattatt cgtggatcgc gctggcactg atcatttatc
     2101 tgttcaccca atcgatcagc gccgtgcgct gggccgcgct cgctcggcca ttgggaatcg
     2161 tgacgccgtt tacagatcta tttaaatatt atctgatcgg tatgttcttc aatcttttcg
     2221 cgcccggcac tgtcggcggc gatgtcactc ggattttcta cctggtgcgc gacgaagaag
     2281 ctcatgcgaa gggccgtgct gtgacgacga cgcatgccgc catatccgtg ctcatggatc
     2341 gcgccatcgg tatgatcgtg ttggtctggt tgggcgccgc gggattgctg ttgtttcccg
     2401 aatacgcggt gccgccgatg gtgcgttcga tcacgctact gctggccgca ggtttcgtcg
     2461 gggcgttatt actactgccg ttggtgcgcc gcgtgctgcc cgagggcggt catcccatag
     2521 ttgtcaaaat tcgcttagct ctgcgtagtt atcgaaccca gtggcgcgcc ctggcggtcg
     2581 ccgctttgct ctctctgatt gttcatttga ttcaagcttg gatgcatcga atcatgggcc
     2641 gggctcttgg tttagacgtg ccgtattcgt tctgtcttat cgtctaccct ctggtcggca
     2701 ctttttccgc gatccctgtg agtttgaatg gcctgggatt acgcgaaggc ggttatatct
     2761 ttatgcttgc cgtcatcggt atcggcagtg agcagggcat agccttcggt cttttgctct
     2821 tcctgatcgt ggcgctcgat agcttgatcg gcggtgtctt gttcctcctg caaaagtcgc
     2881 cgaaagcggg cggcgtgatt gccgagaagg caacttagat caaatagctt cggaacatcg
     2941 tcgccagacc caggaacgag agaaatccaa acgcgtcggt aagacccgta acgataattc
     3001 cggacccgag tgccggatcg agcttcaacg ctttcagtaa caacggtatc accgcaccgg
     3061 caaagcctgc cataaggcca aggttcaaca acatcgcgcc ggcgagaatc agtccgagat
     3121 acggattgcc cttccatagt ccggctgcca ccgcgatcat cgccccggcg gcgacggcga
     3181 tgcagatatt caccgatacc tgtttgacga tggctttcca tgccgaggaa aattccagct
     3241 cgccgagagc gatggcccgg atgatcaccg tcgcggtctg agtggcgccg ttgccgccga
     3301 cgccggcgac caccggcatg aacgttacca gcgcgacgat ttcatggagc gtgccctcga
     3361 acaatccgac gatggaggcc gccaaactcg cggtcagcag gttgaggatg gtccatggca
     3421 ggcgcattct gaccgagcgc gataccggag taaagacacg gtcttcctcg gccaagccga
     3481 ccatcttgta catgtcctcg gtgctttctt ccttgatgat atcgatgaca tcatcgacgg
     3541 tgatgatgcc ttccaaccgg cccccctcat cgacaatggg cagtgctaaa agctcgtact
     3601 tggaaacgat gcgcgcggca tcttcctggt ccatgaaaac atcggctttg atcgggtcgg
     3661 gaatcatcat gtcgcgcaat tttcgcattg gcggcgcgat taccagattg cgaatcggca
     3721 caacccctac cagtttgccg tccttctcga ctacgtaaag ataaaaaaaa ctttctaatt
     3781 cgccgcgctc gcgaatcttg tcgatggcgc cttgggcggt tgcatccggc ggcagcgcca
     3841 tgaagtcggt ggtcatcagg cgccccaccg ttccgtccgg ataattgata atctcacgca
     3901 cggcggcgcg acgttcggga tcgagcagcg caagcacgcg ttcgcggcgg tcttcgggca
     3961 ggctgtcgat gaacgtgacc gcatcatccg gatcggcgcg gacgatcaga cggccgacct
     4021 tttcatcgtc gatcaacgcc agaatgtccg gcaataattc gggcggtagt tctttgagcg
     4081 tcttggcagc acggcgcgat gaaaacagca cgtcgaacag ttggcgcact tcggccgaat
     4141 cgaggccttt gaaaagcaac gcgatgtcgg cggggtggct tttcccgatg aagcgcagga
     4201 cacgatcggg cgtcgcggtg cgcagcattt cgcgaacggt atcgtaatcg atgggagcag
     4261 gtttcatgtg tgcctataag ctggcgaatc gttccttcat agacacagag atggtgtttt
     4321 gcaatgattt gacggtttgt ccacgatttc accatacggg gtaaatttgc tatggtcttg
     4381 aacgcgtcga atttagcggg attaatttca tgcttggaca aaatcaatcg accgcgcgtc
     4441 gctgcatggc ggcgagtctg acggcttacc tgggttggct cgccattcag acgtcgggtg
     4501 caacacacgc ggctaacttg gctctggatg ggagccggcc gccgtcgttt gctagtatcg
     4561 cgaaaaagac catgccggcg gtggtgaaca tttcgactct cacacagcgc tcaccgcgct
     4621 ccggttccaa tgatccgatt gaggattttt tcaatcgatt ttttggtgaa tcgcagcctc
     4681 gggagaacaa ctcgcgcagc ttaggctcgg gaatcctcat cagccgagat ggtgagattc
     4741 tcaccagtta tcacgttgta cggagcgctg acacgatcaa agtcaaatta tcggaccaga
     4801 ccgagcacga agcgcgcttg atcggcaagg acgatcgcac cgacttagca ttgatcaaga
     4861 ttcgccgctc gggcggcaat ttgccctttg cgaagctcgg caattcgaac caattggaag
     4921 ttggcgattg ggtgatggcg attggtaatc cgtttggtct cgaacatact gtcaccgctg
     4981 ggatcgtcag cgccaagggg cgggtgatcg gtgccgggcc ctatgacaat ttcattcaga
     5041 ccgacgcctc gatcaatccg ggcaacagcg gcggcccgct gatcagcgcg gagggagaag
     5101 tgatcggggt caatactgcg attttcagtc aatccggcgg caatgtcggc atcggtttcg
     5161 cgatccccat cgatctggca aaaaaggttt tggatcagct gcgcaagaac ggcaaagtgg
     5221 tgcgtggatg gcttggcgtg cgcgctcagg atctcagtgc cacccaagtg gctgcgctcg
     5281 gaaccatccg aagcgctgcg gagctcgcca tcgtaactga agtgaccgat aagagtcccg
     5341 ccgctgaggc gggcatcaaa accggcgatg tgatcgtcga gtttaacggg aagccggtgc
     5401 ccaggagcag tgaattcccc agtgtgattg ccgacattcc gccgggacaa aaggtctcgc
     5461 taaaaatctt gcgcgagaaa aaagagcaaa ctatcgcagt gcgcataggc gagctcgccg
     5521 atgacgcgga cctcaaccaa caagtcgaag cccgcgatgc cgaacttgga ctgcgagtcc
     5581 agcgtatcac gcctgaagcg gcccggcgct tgggcctaag ctcgcccaag ggggtactgg
     5641 ttgtggaggt gcagccgggc agtccggccg acctcgtggg gattgaaccg gcagatgtga
     5701 ttcgcgaggt taaccaacgc gccgtcacca atgtcaaaga attcgaacgg gcaacccggc
     5761 aaggccggcg cggcgatcgt attctgctgc ttgtgcagcg gggtgacaac gcggtattct
     5821 tcgcggtgaa gcgcaaaggg taggaggact tatgtttgga cttggtgtgc cggaactcat
     5881 ggtgatcctg gtgatcgcgc tcgtgatctt tggcccgagc aagctaccgc agatcggcag
     5941 tgggctcgga aaagcgattc gcgattttaa aaaaggcgtg acggaaaaag acgacgagga
     6001 gacgaatgtc aaaaaagacg gtcaagatct accgcgctga ccgcaatcat taatctagtc
     6061 tctctccctg agcgccaatt ccgccagcgt tcgccggcgc ataattctcg ctcactccat
     6121 ccttcaacga attttaattc ggtcgtaaca aaaacaccgg tgattttact ttgaatcctg
     6181 gatagtgaca gcttgccgcg ctattcagat aagcgggact acagctcgcg cgtggtgcga
     6241 aaaccgggtg cactcttccg gcctaagacg acggtgtaga gggatgtctt attgagcttg
     6301 tacacttttt cgaccatcaa accggttgag gcgataaacg gtttgagttt gaggtcggtg
     6361 cgccagccca gtaaccgggt cagcgggtcg attgcttggg taatcgatcc gattaccggc
     6421 atatcggtgg taaaatgatt caagatcacg atgcgcccgc ttggtttgca gacgcgcttg
     6481 gcctcagcga tcattcgcac cgggtcaggc accacggtga cgacatggaa cgccataaca
     6541 taatcgaacg aattatcggg aaaggttaaa ttgagcgcat ccatttccat gactttgaaa
     6601 tgatgccagc cattttcgtg aattttgtga cgcgcgcgcg aaagcatatc gggcgccaga
     6661 tcgatgccgg tgacgtcgca gtgatgggga tacgcaggaa aggacgtccc agtaccggcg
     6721 ccaatctcga gtatcttcgc gcctatagga atatccaggt tctcaatcac ccgttcgatg
     6781 cggttgtaga agatcttgcc gaagatttta tcgtagaggg gagcgaattc tccgtagagt
     6841 ttactctcgt gcgcctcgat ttcagccatg cgccccaacc ccaatcaacc acgtccggct
     6901 acgcgtcgcg gatatccttc ggttgtttct tgtagaagat tgaaatgtcg cgcttgcgaa
     6961 atcgccattg tttacccttt ttgaaggcgg ggaggatgtt tttcctggcg aattcattga
     7021 cggtgtccgg actcatgtcg agaatcacgg cgacttgttt gctattcagc agctcttctt
     7081 ctcgttccac catagactct ccctttggca aacaccagta ggcgaaaaag gtcgtttttg
     7141 tctaacaagt tcaactaggg ctgtcaaggc aggaatcgcg cagggcaaga tcgcgcagcg
     7201 gcggtggacg atttcaaatc caatcaaagg caatggagcc gtgcaacg
//
LOCUS       DATLCH010000109         7342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9283995, whole genome shotgun sequence.
ACCESSION   DATLCH010000109 DATLCH010000000
VERSION     DATLCH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9283995"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VMZ02_03220"
     CDS             complement(<1..1124)
                     /locus_tag="VMZ02_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR70970.1"
                     /translation="MKLSTFKLLVPIIAVTIQSNSSSTVIPPYLDHMRIPVAAIGTLI
                     SLGPIFALLARLPIGMMYKRGRARWLVSAAIMAMGLTNYSYSFAESSWSFAIVHCLNG
                     FAYSAVTTLYMAFYVDSLAPDENRNHAMGYYVGCLAMGYSTGNFFGGLIADHWGYSWT
                     FQLGALLSFVAVALLWFLHGPSSDASTPGKAKEAGKLTLRESFKALLEPELATVVLVA
                     LFLNLLHQMGGVFISLYGLAVGMSLTQIGIIRAAYAGCNAVTRPISGHVVNKVGHKGL
                     SYFGLPLQALILMLVPLFTGFGTIIAVYVASGFMRAIVLVANAVGLVQDVPESKVRRG
                     LASGVYNAAGDVGNILGPSIGGFIAHATGIASVFVIGSLGS"
     gene            1362..2246
                     /locus_tag="VMZ02_03225"
     CDS             1362..2246
                     /locus_tag="VMZ02_03225"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HUR70971.1"
                     /translation="MDLIRSWMFVPGHRQKMIDKALGLNADAIMLDIEDGVAPNEKES
                     ARTNIGESLGRAKAAGTPARYVRINSIGHPWMAADLAAVVRPGLEGLVCPKVDTVDDV
                     RTVDAMLNDMEPKNQIAKGSVRLLIAIESPRGLFNAPAIAAASSRIVGIIFGTEDFGR
                     EIGLPAVREGEARDLIYARSAIVVAAAAAHVQAVDGVWVDLNDTAGLHLFAKQSRQLG
                     FSAMSCIHPSQVEAINTIFSPTAVEIEYCQKVLQAFEEANARGDGSIAFGGQLIDRPI
                     VERARRTIDMARSLGLLR"
     gene            2280..3212
                     /locus_tag="VMZ02_03230"
     CDS             2280..3212
                     /locus_tag="VMZ02_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR70972.1"
                     /translation="MKKRLSLVLIVIGLCSMGAAAPVRLIPVKITDGVFLLQHSNGSG
                     NSTVVLTNDGVLVLDFAIDSADQIMAFIRKTTDKKVKYLITSHSAGDHASGAWHFRED
                     APAWIATKNQAHDLQMQEREEFLERQKSNDPRNAGYKNGEPLQPTIAIDGPMTLYFGG
                     LTFQITPEARAHSTGDLTVYVPQKRVMLMGDLLDTEIHPGQGESAGVFFSNVKGWIHV
                     LDNIMARNLPVDTYIPGHGPAHIGRGVKDLEEQKRYFIVMRDEVAKMIAAGKTLEQIE
                     KEFKVPQEFAHYTRPERLRSFYKLFYNQLMETGY"
     gene            3239..4288
                     /locus_tag="VMZ02_03235"
     CDS             3239..4288
                     /locus_tag="VMZ02_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70973.1"
                     /translation="MRFTVTTLIVSLSIGGIASAQTKMSTEQLAAYNKPDREKILYAG
                     AKAEGKVTWYTSLAGDSYKKLAAAYEAKYPGVSVESYRATRQEMSARILAESQAQRYI
                     ADTMETTIPLLKLLKDNKWLVPYYFSTIAKYPDNVKEKAAGGLIYWAIDRESHIGLSY
                     NKNLIPAKVVPRNYEGLLRPELKDKIAFAGSDTGVTVTGALLKFKGEEFVRKLKTLNP
                     AVHNVSGRALLDMVISGEVGVSPSTFRNHVEVSLKAGAPIEWIPMDVVPANSGSTAVL
                     AKAPHPNAALLLADFILGPGGQKVLEEYEYGNPTKDYGFKRWYPEQGLSAEQIDALEG
                     KWRATMRDLTKRSSF"
     gene            4372..5841
                     /locus_tag="VMZ02_03240"
     CDS             4372..5841
                     /locus_tag="VMZ02_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR70974.1"
                     /translation="MNTGSYVNGKWYQPAGKEIGRNINPADIDDVIAEFPYASHVDVR
                     KAIDAAQAAFTAWRKTPGPERGRVIWRAAEIARRRADEIARTLTREEGKILKEAKGEV
                     MKGISLLEYYAGEGFRLHGKTLPSEARDTFTYTVRQPLGVVGLIAPWNFPWAIPVWKS
                     APALVAGNTVIFKPSELTPATAALLTEIYEEAGTPAGVFNMLVGTGPGVGAAMVNAPE
                     LRAISFTGSNEVGAALYVKVAERGAKVTCEMGGKNAVIVMPDADLDKAATAIHGGAFG
                     STGQRCTATSRVIAHPDVKQKLVERLVEGAKKIKIGAGLEESIDMGPVVDEQQWSTVL
                     NYIKVGQQEGGKLVLGGKRPEQLSKGYFVEPTIFDNVAPTMRIFKEEIFGPVLAIATA
                     KNLDEALRFANGVEYGLTTSIFTENIDTIMKFVDEVETGMVHVNEPTIGGEAQLPFGG
                     IKSTGVGDREMAEEGLNFFTELKTVFINYSGKAERSMTR"
     gene            5894..7084
                     /gene="chrA"
                     /locus_tag="VMZ02_03245"
     CDS             5894..7084
                     /gene="chrA"
                     /locus_tag="VMZ02_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HUR70975.1"
                     /translation="MENLTKSKNNWPLIKYFLYLGTFGFGGPAALVGFMHRDLVEKRG
                     WLTEDTYKLSLALAQIMPGPLAAQTAIAIGYFEGGVLGATLVGLAFILPSFLMVVAIS
                     LAYVAYGGLWWMQALFYAIGATVIAIIAIAAYKLARSTNKADPLLWGIFVALTGVTVW
                     AQAELAEFFILAGLVVLLARAWPGWKLGMLMTGAAVILGAFIWLFEAWVRQSATATDS
                     QDVLTQILIFFTKAGAFVFGSGLAIVPFLQQGVVQQFGWLNEHQFLDAVAVAMITPGP
                     VVITVAFIGYLVAGFAGAIMASIGIFLPVYFFTIIPAPWFRRHRDNPQLKAFVNGATA
                     AATGAITGAVIVLAVRAITDWPTAIIALVSFGVLWRYKISEPIIVTISGLIGLILWPL
                     LRGG"
     gene            7081..>7342
                     /locus_tag="VMZ02_03250"
     CDS             7081..>7342
                     /locus_tag="VMZ02_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70976.1"
                     /translation="MIPAIRSSLRHAAKKLTYVKKDSMGHDSARRIAILYPGDYAERV
                     NATTENSRFVDLFRAFVAEGIHAEPAVYRDDFCSEVHDQLMQV"
BASE COUNT         1723 a         2059 c         2009 g         1551 t
ORIGIN      
        1 gatcctaacg agccgataac aaacacgctg gcgatgccgg tggcatgagc gataaagccg
       61 ccgatggacg gaccgaggat attgcccacg tcgccggcgg cattgtaaac cccggaagcg
      121 agaccgcgac gaactttgct ctcgggcacg tcctgcacca gaccgaccgc gttggccacc
      181 aggacaatgg cgcgcatgaa tcccgacgcg acataaacgg cgataatcgt gccgaagccg
      241 gtgaacaacg gcaccagcat cagaatcaaa gcttgcaaag gcaagccgaa gtaagacaag
      301 cctttgtgcc ccaccttatt taccacatga ccgctgatag gtcgggtcac ggcattgcat
      361 ccggcataag cggcacggat gatgccgatc tgcgtcaaac tcatgcccac cgccaatccg
      421 taaagggaga taaacacacc gcccatttga tgcagcagat ttaaaaagag cgcgaccaga
      481 accacggtcg ccaactcggg ttcgagaagc gccttgaacg attcccgcag cgtcagctta
      541 cccgcttcct tggctttgcc cggagtcgac gcgtcgctgc ttggcccgtg caaaaaccac
      601 aacagcgcaa cggcgacaaa agagagcagc gcgcccaatt gaaaagtcca cgaatagccc
      661 caatggtcgg caatcagccc accgaaaaaa ttccccgtcg agtaacccat cgccaaacag
      721 ccgacatagt agcccatggc gtggttgcga ttttcgtcag gcgcgagcga atcgacatag
      781 aatgccatgt acagcgtggt caccgcgctg taagcaaagc cgttgagaca atgcacgatg
      841 gcaaacgacc aactgctttc ggcaaagctg taactatagt tggttaaccc catcgccata
      901 atcgcggccg aaaccaacca gcgcgcgcga ccgcgcttgt acatcatgcc gatgggcagg
      961 cgcgccaaga gcgcaaatat cggtcctagc gaaatcagcg taccgatcgc agccacggga
     1021 atgcgcatgt gatcgagata aggcggtata accgtcgatg acgaattgct ttggatcgtc
     1081 actgcgatga tcggcaccaa gagtttgaag gtgctgagtt tcataacatt ccgttattcg
     1141 ctaaaacttt gtggtcttca accgcagcgc cggaaagatt tctcactttg ttcgcaatga
     1201 taaacgaaag cggctcacta acgaattgtc atctcgaacg aagtgagaga tctctcccgc
     1261 atcagcgcaa tcagcacgaa tgacagcgct ggtgatcttc atgtcgcaac gttagacatt
     1321 cggtgcctct tcaggtaaaa atttcgccgg aggagattta gatggacttg atccgctcgt
     1381 ggatgtttgt gcccggacat cggcaaaaga tgatcgacaa agctctgggc ctcaatgccg
     1441 acgcgattat gttggacatc gaagacggcg ttgcacccaa cgagaaggaa tcggcgcgaa
     1501 caaatatcgg cgagtcattg ggacgggcca aagccgccgg cactccggcc cgctacgtgc
     1561 gcatcaattc cattggccat ccctggatgg ccgccgattt agcggccgtg gtgcgccccg
     1621 gtttggaagg tttggtttgc cccaaagtag acaccgtcga tgacgtccga acagtcgacg
     1681 ccatgctcaa tgacatggag ccgaaaaacc aaatcgccaa gggcagcgtc cggctgctaa
     1741 tcgccatcga aagccccaga ggtctattca atgcgccggc catcgctgcg gcttcatcgc
     1801 gtattgtcgg catcatcttc ggcaccgagg acttcggtag ggagatcggc ctccccgcag
     1861 tgcgcgaagg cgaagcacga gacctaatct acgcgcgctc ggcgattgtc gtcgcagccg
     1921 cggcggccca cgtgcaagcc gttgacggcg tttgggtgga tctgaatgat acggcaggtt
     1981 tgcatctgtt tgctaaacaa tcgcggcagt tgggattctc ggcaatgtca tgcattcatc
     2041 cctcccaggt cgaagcgatc aatacgattt tcagtccgac ggcggtggag atcgaatact
     2101 gccaaaaagt cctgcaggct tttgaagaag ccaacgcacg cggagacggc tcgattgcat
     2161 tcggtggcca gctcatcgac cggcccatcg tcgagcgtgc ccgtcgcacc atcgatatgg
     2221 ccagatcgct gggcctactc cgataaagtg actcctctga tgcgaaggaa atcaaatcga
     2281 tgaaaaaaag gttgtcgctt gtactgatcg ttataggttt gtgcagtatg ggcgcagccg
     2341 cgccggtgcg cctgattccg gtcaaaatca ccgatggtgt tttcctgctg cagcattcca
     2401 atggctccgg caattccacc gtggtgctta ccaatgacgg cgtgttggtg ctcgatttcg
     2461 ccatcgacag cgcggatcag atcatggcat tcatccgcaa aaccactgat aaaaaggtca
     2521 agtatctaat cacctcacat agcgccggag atcacgcctc gggcgcctgg cattttcgcg
     2581 aagacgcgcc ggcctggatc gccacgaaaa atcaagcgca tgatttgcag atgcaggagc
     2641 gcgaagaatt tcttgagcgg caaaagtcca acgacccacg caatgccggt tataaaaacg
     2701 gcgaaccttt gcaaccgacg atcgccatcg acggcccaat gacgctttac ttcggcggat
     2761 tgacctttca gatcaccccc gaagcacgtg cccatagcac cggagatctc acggtttacg
     2821 tcccgcagaa gcgggtcatg ttgatgggcg atctcctcga tacggaaatt catccggggc
     2881 aaggcgagtc tgccggagtg ttcttttcca acgtcaaggg ctggattcac gtactcgaca
     2941 acattatggc gcgtaacttg cccgtcgata cgtacatccc gggccatggg ccggcgcata
     3001 ttggccgcgg cgtcaaagac ttggaagagc agaagcgcta ttttatcgtg atgcgcgatg
     3061 aggtcgccaa gatgatcgcg gcgggcaaaa cattggagca aatcgaaaaa gagtttaagg
     3121 tgccccagga gttcgctcac tatactcggc ccgaacggct gcgctcgttt tacaaacttt
     3181 tttacaacca actgatggag accggctact agtcacaccg gtcttaggag gctgaaagat
     3241 gcgtttcacg gtgacaactc taattgtctc tttaagcatc ggtggtatcg cttcggcgca
     3301 aaccaagatg tccaccgagc aattggcggc ctacaataaa cccgaccgcg agaagatcct
     3361 ctacgccggc gcgaaagccg agggcaaggt gacctggtac acatcgctgg ccggcgattc
     3421 gtacaaaaaa ctagccgccg cttacgaggc caagtatccc ggcgtctccg tggaatctta
     3481 ccgcgccact cgccaggaaa tgagcgcacg catcctggcc gagtcccagg cgcagcgcta
     3541 catcgccgat accatggaaa cgacgattcc gctgctgaag cttctgaaag acaacaagtg
     3601 gttggtgcct tactattttt cgacaattgc caagtatccc gataacgtca aggaaaaggc
     3661 tgcgggcggc ctaatctatt gggccattga ccgggaatcc catatcggtc tctcctataa
     3721 taagaatctg attcccgcta aagttgtgcc gcgcaactat gaagggctgc tgcgacccga
     3781 gctaaaagac aagattgctt tcgccggcag cgataccggc gtcacggtta ccggcgctct
     3841 gcttaaattc aaaggtgaag agtttgtccg caaactcaaa actttgaatc cagcggtcca
     3901 taacgtttcg gggcgcgccc tgctcgacat ggtcatttcc ggtgaagtcg gtgtttcgcc
     3961 gagcactttt cgcaatcacg tcgaagtttc cctcaaagcc ggcgcaccga tcgaatggat
     4021 acccatggac gtagtcccgg caaattcagg ttcgaccgcg gtgctagcca aggcgccaca
     4081 tcctaatgcc gcgctgctgt tggccgattt cattcttgga ccaggcgggc agaaagtgct
     4141 cgaagaatat gaatacggca atccgacaaa agattacggt ttcaaacgct ggtatcccga
     4201 acaaggtctc agtgccgagc aaatcgatgc tctcgaaggc aagtggcgcg caaccatgcg
     4261 cgatttgacc aaacgctcat ctttctagtc gttgatgaaa catagctgca aacagagcgc
     4321 cgctccggcg ctctgttttt tttgtgcgac agataaagct gcgaggttcc catgaatacc
     4381 ggttcctacg ttaacggcaa gtggtaccaa cctgcgggca aagaaattgg ccgcaacatc
     4441 aatcccgccg acatcgacga tgtcatcgcg gagtttccct acgcgagcca tgtcgacgtg
     4501 cgaaaggcca tcgatgcggc acaagcagcg tttaccgctt ggcgcaagac tcccggccct
     4561 gaacgcggtc gagtcatttg gcgcgccgcg gaaatcgccc gccgccgcgc ggatgaaatt
     4621 gcccgcacgc tcactcgcga agaaggcaag attttaaaag aagccaaggg cgaagtgatg
     4681 aaaggcatct cgctgctcga atactatgcc ggcgaaggct ttcgcttgca cggcaagaca
     4741 ctgccgtccg aagcgcgcga cacctttacc tacacggtgc gccaaccatt gggtgtcgtc
     4801 gggttgatcg cgccgtggaa tttcccttgg gccatcccgg tatggaaaag tgcgccggcg
     4861 ttggttgccg gcaataccgt gatattcaaa ccgtcggagc tgacacccgc caccgccgcg
     4921 ctactgacgg aaatttatga ggaagccggc acccctgccg gcgtattcaa catgcttgtc
     4981 ggcaccggcc ctggggtcgg cgcggcgatg gtcaatgcgc ccgagctgcg cgccatttct
     5041 tttaccggct ccaacgaagt cggcgccgca ctctacgtca aagtcgcaga gcgcggcgcc
     5101 aaagtcactt gcgaaatggg cggcaagaac gcggtcatcg tcatgcccga cgccgatctt
     5161 gataaagccg cgacagcgat acacggcggc gccttcggct ccaccggcca acgctgcacc
     5221 gccacgtcac gcgtgatcgc gcatccagat gtcaaacaaa agctggtgga acgcttagtc
     5281 gaaggcgcca agaaaataaa aatcggcgcc gggcttgagg aatcgataga catgggacct
     5341 gtagtggacg agcagcaatg gtcgacggtg ttgaattata tcaaggtcgg ccagcaggaa
     5401 ggtggcaaac tcgttctcgg cggcaagcgg cccgagcagt tgtcaaaagg ctacttcgta
     5461 gagccgacca tcttcgacaa cgtcgcgccg accatgcgca ttttcaagga ggaaatcttc
     5521 ggcccggtgc tggcgattgc aacggcgaaa aatttggacg aggcgctgcg cttcgccaac
     5581 ggcgttgaat acggcctgac cacgtctatc ttcaccgaaa acatcgacac catcatgaag
     5641 ttcgtcgacg aagtcgaaac cggcatggtt cacgtcaacg agccgaccat cggcggcgaa
     5701 gcccagctgc cgtttggcgg aattaagtcg accggtgtcg gcgaccgcga gatggcggag
     5761 gaaggtttga atttttttac cgaactgaaa accgtcttta tcaattactc cggcaaagcc
     5821 gagcggtcga tgacacgata gccgaagcac gcctgatgga cttaattcaa cgttagactt
     5881 cgattataaa gccatggaaa acttgactaa atctaaaaat aactggccgt tgatcaaata
     5941 ttttctctac ttgggaacat ttggatttgg cggcccggca gccttagtcg gatttatgca
     6001 tcgcgatttg gttgaaaagc gcggctggct aaccgaagac acctacaagc tgtcgttagc
     6061 gctcgctcag atcatgccgg gaccactcgc cgcgcagact gcgattgcca tcggttactt
     6121 tgaaggtggt gtactcggcg cgacgttggt cggtcttgct ttcatccttc cttcgtttct
     6181 catggtcgtc gcgatttcgc ttgcctacgt tgcttacggt gggctctggt ggatgcaggc
     6241 tctgttctat gcgatcggcg ccacggtcat cgcgatcatt gccattgccg cttacaagct
     6301 cgcgcgcagc accaataaag ccgatccatt gttgtggggc attttcgttg cgcttaccgg
     6361 cgtgaccgtg tgggcgcaag cagagctggc ggagtttttc attcttgccg gcttggtagt
     6421 cttgcttgcc cgggcatggc caggttggaa gctgggcatg ttgatgacgg gcgcagcggt
     6481 tattttaggt gcattcatct ggcttttcga agcttgggtc agacaatccg caactgcgac
     6541 ggatagtcag gacgtcctca cgcagatcct gatatttttc accaaagcag gagctttcgt
     6601 tttcggcagt ggcctcgcaa tcgtgccgtt tctacaacaa ggcgtggttc agcagttcgg
     6661 ctggctcaat gagcatcagt ttctcgacgc agtcgccgtc gcgatgatca cgcccggtcc
     6721 cgtggtgatc acagtcgcgt tcattggata cctcgtcgcc ggttttgccg gtgcgatcat
     6781 ggcgtccatc ggcattttct tacccgtata ttttttcacg atcattccag cgccatggtt
     6841 cagaagacat cgagacaatc ctcaactcaa ggcgttcgtc aatggcgcga ccgccgccgc
     6901 taccggggcc attaccggtg ccgtcatcgt tctggctgta cgcgcgatca ctgattggcc
     6961 gacggcaatc atcgcgctag ttagtttcgg tgttctctgg cgctacaaaa tctccgaacc
     7021 gatcattgtg accatttccg ggctgatcgg attgattctc tggccgctgt tgcgcggcgg
     7081 atgattccag ctatccgaag cagtctcaga catgcagcca agaaattgac ttacgttaag
     7141 aaagattcta tggggcacga ttccgctagg aggattgcaa tcctttaccc tggcgattac
     7201 gccgagaggg taaacgcgac aactgaaaat tcccggttcg tagacttgtt ccgcgccttt
     7261 gtggccgaag gcatacacgc ggaacccgcg gtttaccgcg atgatttttg ctctgaagtt
     7321 cacgatcaac tcatgcaagt tg
//
LOCUS       DATLCH010000110         4283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1836025, whole genome shotgun sequence.
ACCESSION   DATLCH010000110 DATLCH010000000
VERSION     DATLCH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836025"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VMZ02_03255"
     CDS             <1..696
                     /locus_tag="VMZ02_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70977.1"
                     /translation="MMLDMQNPKHPEEVMRWWMPGQWVAGGETPNFKGADTRCHHPIR
                     RGDRLYISYWHGGFAIVDISDLSKPRTVSTLDWSPPYPCPTHTTLPLLNRIMERDWLI
                     VTDEEVGEKLNETHNAFLWVVDITKEDLPLPVATFIVPFEGKSTSHSRFGAHQPAEQV
                     YGNTLYVTWFAGGLRAVDFSNPYQPKEVGYYVPNPGKNQNVVMSNDVFHDKDGKLYLI
                     DRYDGLEILEPQI"
     gene            839..1432
                     /locus_tag="VMZ02_03260"
     CDS             839..1432
                     /locus_tag="VMZ02_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR70978.1"
                     /translation="MKLYNVAYSGNSYKVRLLLAHLKIVCEIIEVDILNGASRTAEFL
                     KINPNGRTPVLDDGGFILAESNAILAYLAKGTKYLPDDRRTFGSILQWMFFEQYSHEP
                     FIATSRFWKQHKPDSNEKTALLASKRDGGWAALRVMEGHLANNDFFAGDYSIGDIALF
                     AYTHVADEGGFPLTAFPHVRGWIKRITEQANFVPMSS"
     gene            complement(1502..3211)
                     /gene="ybaL"
                     /locus_tag="VMZ02_03265"
     CDS             complement(1502..3211)
                     /gene="ybaL"
                     /locus_tag="VMZ02_03265"
                     /inference="COORDINATES: protein motif:HMM:NF007950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HUR70979.1"
                     /translation="MPHNLTLITTIVAALGTALAFGFLAARLRLPVLIGYLAAGILLG
                     PFTPGFVADLELSAQLAEIGVMLLMFGVGLHFSIDDLLAVRKIAVPGAIVQIAVATVL
                     GIAVTSLWGWTIAEGFVFGLALSVASTVVLLRALEGRGILETVNGRIAVGWLVVEDLV
                     TVLVLVLLPPLSLWLGGSGQNQAGGQELLSTLAWTLGKVALFVALMVIGGRRIFPWIT
                     WHVARIGPRELFTLCIIAIAVGIAYGAAVMFGVSFALGAFIAGMVMRESPLSHRAAEE
                     SLPFRDAFSVLFFVSVGMLFDPQVLIREPYHVLAVVGIVVVGKSLAAFGLVMLFRYPL
                     NTALTVSAGLAQIGEFSFILGQLGLSLGILPKEARSLILVGGIISIALNPVVFHAIEP
                     LQAWIRAKSKRAQLFEHPDDPLATLPMTIDLKRLTGHVVLVGYGRVGRRIGNELEAQG
                     ISFVLAEENREIITAAREKGLAAVSGDATEPMVLVQAHIQRARMLVIAIPDTVNVRRM
                     IEIARTVNPRIEAVVRTHSEEEAALLEKESAGKIFLGERELARSMSEYVVRRYAAKVE
                     QTS"
     gene            complement(3339..4238)
                     /locus_tag="VMZ02_03270"
     CDS             complement(3339..4238)
                     /locus_tag="VMZ02_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HUR70980.1"
                     /translation="MPELPDITIYIEALQARLLNQPLEKIRLGNPFLLRTFEPPIGAA
                     HGKKVVGFERIGKRIVVELEGELFLVFHLMITGRFHWKKRGIAVPRKYGHAAFDFPMA
                     TLLLTEMGTKKRASLHLVQGREQLREHDPGGLEIFDADLQSFRAALLRENHTLKRTLT
                     DPHVFSGIGNAYSDEILHRAQLSPIKQTKYLSGDEIARLFQATRDCMAEWIERLREDT
                     GREFPERVTAFRDEMAVHGKYRKPCPVCGAPVQRIVYADNETNYCAQCQTGGKLLADR
                     SLSRLLKDDWPRTLEEMEEGRRS"
BASE COUNT         1030 a         1267 c         1137 g          849 t
ORIGIN      
        1 atgatgctcg atatgcaaaa tcccaaacat cccgaagaag tgatgcgttg gtggatgccg
       61 gggcaatggg tggccggcgg cgagacgccc aatttcaaag gcgccgatac ccgctgccac
      121 cacccgatcc ggcgtggcga tcggctttac atcagctact ggcatggcgg ctttgccatc
      181 gtcgatattt ccgatctcag taagccgcgc accgtcagca ccctcgattg gagtccgccc
      241 tatccctgtc cgacccatac gacgttgccg cttctgaatc gcatcatgga gcgcgactgg
      301 ttgatcgtca ccgacgagga agtcggtgaa aaattgaatg aaactcacaa cgcgtttctc
      361 tgggtggtcg atatcaccaa agaagatctg ccgctgcccg tcgctacgtt catcgtcccg
      421 ttcgagggca aatcgacaag tcatagccgc ttcggcgcgc accaacccgc cgagcaagtc
      481 tacggcaata cgctctacgt cacctggttt gccggcggtt tgcgcgcagt cgatttctcc
      541 aatccatacc agccgaaaga agtcggctac tacgtcccca accccggtaa aaatcagaat
      601 gtcgtcatga gcaacgacgt cttccatgac aaagacggca agctgtattt gatcgaccgc
      661 tatgacgggt tggaaattct ggaaccgcag atctagctga ggttcaaaac gttcaatccg
      721 ttcaaccgct tcgctccgtt caatccccca cctctgtcct cccccgcgac gcggggggga
      781 tgtcaggagg gggcttgagc gatttgaacg gctcgaacgt tttgaacgga agttgttgat
      841 gaaactctac aacgtcgcct attcggggaa ttcgtataaa gtgcgactcc tgctggcaca
      901 cttaaaaatt gtctgcgaga tcatcgaagt cgacatcctg aatggcgcaa gccgcacagc
      961 tgagtttctc aagatcaatc ccaacggccg cacgccggta ctcgatgacg gaggctttat
     1021 cctggcggaa tcgaacgcta tcctcgccta cctcgccaag ggaactaaat atctccctga
     1081 cgatcgccgg acatttgggt caatcttgca atggatgttc ttcgagcagt acagccacga
     1141 accgttcatc gctacctcgc gtttctggaa acagcacaaa cccgactcca acgagaaaac
     1201 cgctctgctg gcgtcaaaac gcgacggtgg ctgggctgca ctgcgggtga tggaaggtca
     1261 tttagcaaac aatgattttt tcgccggcga ttattccatc ggcgacatcg ccctgttcgc
     1321 ttatacgcat gtcgcagacg aaggcgggtt tccgttaact gcttttccgc atgttcgcgg
     1381 ctggataaag cgtataacgg aacaggctaa ctttgtcccg atgtcgtcct agcaaccgat
     1441 ttgacgccga cctgcggcgg gcaacgccat atagcattcc gccgccaacg ccgactgact
     1501 gctagcttgt ctgctcgact ttagccgcgt agcggcgcac tacgtactcg ctcatactcc
     1561 gcgccagctc gcgttctccc agaaagattt tcccggcgct ttctttttcc agcaacgccg
     1621 cctcttcctc gctgtgcgtg cgcacgactg cttcgatgcg cgggttgaca gtccgggcga
     1681 tttcgatcat ccggcgcaca ttaacggtgt ccgggatggc tatgaccaac atgcgcgcgc
     1741 gctgaatgtg ggcctgaacc agcaccatcg gttccgtcgc atctcccgat acggccgcca
     1801 gtcctttttc ccgtgccgcc gtgataattt cacgattttc ctcagcgagg acaaacgaga
     1861 tcccctgcgc ctcgagctca tttccgatac gtcgccccac cctgccgtaa ccgaccagca
     1921 cgacgtggcc agtgagccgc ttgagatcga tggtcatcgg cagtgtcgcc agcggatcgt
     1981 cgggatgttc gaaaagttga gcgcgtttcg atttggccct aatccatgct tgcagaggct
     2041 cgatggcgtg aaagaccacc gggttgaggg cgatcgaaat aatcccaccg acaagaatga
     2101 gactgcgggc ttctttcggc aatatcccaa gcgacagtcc caattggcca aggatgaagg
     2161 aaaattcacc gatctgggcc aacccggccg agacggttag agcagtattg agcggatagc
     2221 gaaacagcat gactaggccg aaagcagcga gagatttgcc gaccacgacg atgccgacaa
     2281 ctgcgagcac gtggtagggc tcgcgaataa gcacctgggg atcgaagagc ataccgaccg
     2341 aaacgaaaaa cagcacggaa aaggcatccc tgaagggcag cgactcctcc gccgcccggt
     2401 ggctcaacgg tgattcgcgc atcaccattc cggcgatgaa cgcacccaga gcaaacgaga
     2461 ctccgaacat gaccgcggcg ccataagcga tgccgaccgc aattgcgatg atgcataaag
     2521 tgaagagttc gcgcgggccg atccgcgcga catgccaggt gatccagggg aaaatgcgcc
     2581 ggccaccgat taccatcaaa gcgacaaaaa gcgcgacttt gcccagagtc cacgccagag
     2641 tggacaacaa ctcttgaccg ccggcttgat tctgcccaga accgccgagc cacaaagaaa
     2701 gcggcggcaa cagcaccaaa acgagcaccg tgaccaaatc ctcgaccacc aaccaaccaa
     2761 ccgcgattcg tccgttaacc gtttctaaaa ttccccggcc ttccaaggct ctgagaagca
     2821 caaccgtact tgcgacggat aacgccaggc cgaacacgaa cccttctgct atggtccaac
     2881 cccagagact cgtaactgca ataccgagca ccgtcgcaac agcgatctgc acgatcgccc
     2941 ccggaacggc gattttgcga accgccagga gatcatcgat ggaaaagtgc agtccgacac
     3001 cgaacatgag caacatgaca ccgatctcag cgagctgagc tgagagttcg agatcggcaa
     3061 caaagcccgg agtaaatgga ccgaggagga tcccggcagc gagataaccg atcaacaccg
     3121 gaaggcgaag ccgagcggct agaaaaccga aagccaatgc tgtgccgagc gcagcaacga
     3181 tggtggtgat cagcgtaaga ttatgcggca actgcagggc tcctctcggt ccgctcaatg
     3241 aagctgagtc gactgacgac actcatgcgc ggcacaggac gaaacacatg cgagaagata
     3301 ctataacgtc gagctagggc aatacagcga gcatcaatct acgaacgacg cccctcctcc
     3361 atctcctcca acgtccgcgg ccagtcatct ttgagcagac gcgacaaact gcgatcggcg
     3421 agcaatttgc cgccggtctg gcactgcgca caatagttgg tctcgttatc ggcgtagaca
     3481 atacgctgca ccggcgcgcc gcacaccgga catggtttgc gatatttgcc gtgaaccgcc
     3541 atctcgtcgc ggaatgcagt cacgcgctca ggaaattctc tgccggtatc ttcgcgcaag
     3601 cgctcgatcc actccgccat gcaatcccgc gtcgcttgaa ataatcttgc gatttcgtcg
     3661 ccgcttaagt atttggtctg ctttatcggc gacaactgcg cgcggtgcag tatctcgtct
     3721 gaatacgcgt tgccgatacc gctgaacacg tgaggatcgg ttaaggttct tttcaaggta
     3781 tgattctcgc gcaggagcgc ggcgcgaaat gactgcaagt cagcatcgaa aatttccagt
     3841 ccgccaggat catgctctcg gagttgctct cgcccctgga ccagatgcag cgaagcccgc
     3901 ttcttggttc ccatttccgt cagcagcagc gtcgccattg gaaaatcaaa agccgcgtga
     3961 ccgtacttgc gcggcacggc gatgccgcgc ttcttccagt gaaacctccc ggtgatcatg
     4021 agatgaaaaa ccaggaacag ctcgccttct agctcgacga caatgcgctt gccgatccgc
     4081 tcaaaaccaa caactttttt gccgtgcgcg gcgccgatcg gcggctcaaa ggttcgtaag
     4141 agaaatggat tgccgagacg gattttctcg agaggctgat tgagcaaacg agcctgcagt
     4201 gcttcgatgt aaatggtgat gtcgggcagt tccggcatgg ctgaaaaaat tgataatgga
     4261 gaatggagaa ttgaaaatta aag
//
LOCUS       DATLCH010000111         7077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4057090, whole genome shotgun sequence.
ACCESSION   DATLCH010000111 DATLCH010000000
VERSION     DATLCH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4057090"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VMZ02_03275"
     CDS             <1..398
                     /locus_tag="VMZ02_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HUR70981.1"
                     /translation="VSPKASSLELYKGLLGGSAHGVFNGKIVVHKDAQKSDARQTNKN
                     LLLSDSAVIDTKPQLEIYADDVKCSHGSTIGQLDADAVFYLRSRGLDPAAARSLLSFA
                     FASDVVRRLGIEKLRQRLDDYLVARFGAW"
     gene            415..1659
                     /locus_tag="VMZ02_03280"
     CDS             415..1659
                     /locus_tag="VMZ02_03280"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HUR70982.1"
                     /translation="MSQKRTAPVKFDVARVRQDFPILSQKVHGKPLVYLDNGATSQKP
                     LCVIEAIDHYYKAENSNIHRGVHYLSERATAAYESARGKLRGFINARSDQEIIFVRGT
                     TEAINLVAQSYGRTFLKAGDEIIISAMEHHSNIVPWQMLCDQVGAKLRVIPINHDGEI
                     VMEEYEHLLNDKTKFVAVTHVSNALGTIVPVKRIVALAHGRGIPVMLDGAQAVPHLQV
                     DVQDLDCDFYAFSGHKMFGPTGVGVLYGRAELLEKMPPYQGGGDMISLVTFEKTHYNV
                     LPYKFEAGTPNVADGIGLGAAVDYLRALDWPAVAAHEDRLLRYATAALQQIAALRIIG
                     TAKEKAGVLSFVFDHVHAHDVGTILDQEGVAVRAGHHCAMPVMQRFGVPATARASFAF
                     YNTVEEIDLLVRAIERVLKVFD"
     gene            1659..2114
                     /locus_tag="VMZ02_03285"
     CDS             1659..2114
                     /locus_tag="VMZ02_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HUR70983.1"
                     /translation="MSELDDLYQEVILDHNKAPRNFRAISGANRRAEGYNPLCGDQVT
                     VYLQVEDGVIKDISFQGSGCAISKASASMMTTELKGKPESAAHELFENVHNMLTGELD
                     NKGGGSERVGKLAILSGVCKFPARVKCASLAWHTMETALKGEKHPVQTE"
     gene            2144..2692
                     /locus_tag="VMZ02_03290"
     CDS             2144..2692
                     /locus_tag="VMZ02_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HUR70984.1"
                     /translation="MTTDKPITLIRDCSAVMVPSGEPLSLKCGSQVWLTQSLGGGYTV
                     MTDHGYTVRVDGRDGDALGFSDGQATRGQSIEKKGSLQERVWQQLRSCFDPEIPVNIV
                     DLGLIYDCRVEPLPNGGHKALVLFTLTAQGCGMGEFLKADIRRKLLEVSDLSEAAVEL
                     VWDPPWSQSRMSVAAKHQLGIE"
     gene            2703..3023
                     /locus_tag="VMZ02_03295"
     CDS             2703..3023
                     /locus_tag="VMZ02_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HUR70985.1"
                     /translation="METINQTIIEAEVIEALRTVFDPEIPVNVYELGLIYLVEVSSEG
                     AVMIRMTLTSPHCPAVQSMPAEIESKVRTISGVTDVNIDLVWDPAWDQSKMSEAARLQ
                     LGMM"
     gene            3073..3483
                     /locus_tag="VMZ02_03300"
     CDS             3073..3483
                     /locus_tag="VMZ02_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HUR70986.1"
                     /translation="MTPTSFEQLGGEAKLRAVIDTFVDRVFADRMIGFFFRSADKARI
                     KEMEYQLAANFLGAKIEYGGRPLDQVHRKFPIMGGHFARRRQILKESIEAHGVPDEIK
                     KLWLDHTDALRSLITPENDSGCDPIAARERVKTA"
     gene            3585..4448
                     /gene="metF"
                     /locus_tag="VMZ02_03305"
     CDS             3585..4448
                     /gene="metF"
                     /locus_tag="VMZ02_03305"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HUR70987.1"
                     /translation="MRLAELYSQPRLTLSVEFFPPKTEKGEENLFSEIELIKRINPAF
                     CSVTYGAGGSTREKTVDLVDTIRSGCGLEVMCHLTVVGQSKDDARAVLKKLQTCGIEN
                     LLALGGDPPQGSSDWQPHPDGFHHAIELVREAVAMRSFSVAVAGFPEIHPRALSRDAD
                     LQFLKEKVAAGADAIITQLFFDNQDFYRFSEDLRKLGVVVPIIPGVLPLQSAPQVRRF
                     TSLCCSKIPDKLERELIKVENDEAAAVALGIDYATQQCEELIKFGVPGLHFYSLNKSH
                     SVLAIHKNLGL"
     gene            4511..6016
                     /gene="lysS"
                     /locus_tag="VMZ02_03310"
     CDS             4511..6016
                     /gene="lysS"
                     /locus_tag="VMZ02_03310"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HUR70988.1"
                     /translation="MADDQPILTEDDNQQVEVRKQKLAKLRDGAQVVYPNDFKPSHSV
                     TDVFAQASNLSDDELHANSIAVSVAGRIMAIRRMGKASFFQIQDRRGRLQIYARKDKI
                     GDDAYELFQTLDIGDLVGVSGHVFRTKTKELTVEAAGLRLLAKCLRPLPEKWHGLADV
                     EARYRQRYVDLMVNPEVRDVFEKRSRIVRLLRQFFEARDFLEVETPMMQSIPGGAAAK
                     PFTTHHNALDLELYLRVAPELFLKRLLVGGFERVFELNRNFRNEGISVRHNPEFTMVE
                     FYQAYATFEDLIQLTEELFVVIANDIAGGLTVPYNGATIELTRPWQRLTIRDAVMVHG
                     GAQEKDVASLESLRTFAQQSGLKIDLNVPYGKLLVAVFEEVAEAKLIQPTFLTGFPIE
                     VSPLARKNDRNPAMVDRFELYIGGRELANAFSELNDPFDQRQRFLEQMQARAAGDEEA
                     NPIDDDYVRALEYGMPPAAGEGIGIDRLVMFFTNSPSIRDVILFPLLRPHR"
     gene            6037..>7077
                     /locus_tag="VMZ02_03315"
     CDS             6037..>7077
                     /locus_tag="VMZ02_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR70989.1"
                     /translation="MRYELFIGLRYLQARRRETFISLITLISICGVMIGVMTLNVVMS
                     VMTGFEETLRDRLLGINAHIALVRSGEPMRDYEKLAQQMSKEPGVVAASPTVYGQVML
                     TAGSRVTGVVVRGVDPDRVNGVVNIQSFMREGNLDGLKTQHPIQVDERTVSLPGIILG
                     ERLANQLGVFRGNAVQVVSPLGSPTAIGVIPKVRRFVVSGILKSGMSEIDSTLVFMSL
                     ADAQKFFELGDGVTNIEMRVQDVSRSREIADRIQRQLGFPYFAEDWTRLWPNLFSALK
                     LEKTVYFLVLLLMVLIGAFNIVSTLVMVVMEKRKDIAILRSMGATQKSIRQIFLLKGC
                     LIGVVGTILGVIL"
BASE COUNT         1564 a         1830 c         2034 g         1649 t
ORIGIN      
        1 atgtcagtcc gaaagcatcg agtctcgaat tgtacaaagg cttgcttggc ggttcggctc
       61 atggtgtttt taatggcaag atcgttgttc acaaggatgc gcagaagtcg gacgcgcgcc
      121 agaccaacaa gaatttacta ctgtcggaca gcgccgtgat cgataccaag ccgcagttgg
      181 aaatttatgc cgacgacgtc aagtgcagcc atggttcgac catcggccag ctcgacgccg
      241 acgcggtctt ttatctgcgt tcgcgcggtt tggatccggc tgcggcgcgc agtttgctga
      301 gctttgcctt tgccagcgac gtggtgcggc ggttgggtat tgaaaaactg cgccaacggc
      361 tagacgatta tctggtcgcc cgcttcggag catggtgatc ggcttatgaa agcgatgtcg
      421 cagaagagga ccgccccagt caagttcgat gttgcccggg tgcgccagga ctttcccatt
      481 ctcagtcaga aggtccacgg caagccgctg gtctatttgg acaacggcgc cactagccaa
      541 aagccgctgt gtgttatcga agcgatcgac cattactata aggccgaaaa ttccaacatc
      601 catcgcggcg tgcattatct gagtgagcgc gccacggcag cctacgagtc ggcgcgtggc
      661 aaactgcgcg gctttatcaa cgcgcgttcc gatcaagaaa ttatctttgt acgcggcacc
      721 accgaagcga ttaatttggt ggcgcagagt tacggccgaa cttttttgaa agcgggcgac
      781 gagattatca tctcggcgat ggagcatcac tccaatattg tgccgtggca gatgctttgt
      841 gatcaggtcg gcgccaagct gcgggttatt cccattaacc atgacggcga gatcgtcatg
      901 gaagagtatg aacacttgct caacgacaag accaaatttg tggccgttac tcatgtctcc
      961 aatgcgcttg gcaccatagt tccggtgaaa cggattgtcg ccttggcgca tggtcggggg
     1021 atcccggtaa tgctcgacgg cgcccaagcc gtgccgcatt tgcaagtcga cgtgcaagat
     1081 ctagattgcg atttttacgc cttctccggt cacaagatgt tcggaccgac cggagtcggc
     1141 gtattgtacg gccgcgcgga gctcttggaa aaaatgccgc cctatcaggg tggcggtgac
     1201 atgatcagct tggtcacttt tgagaagacc cactacaacg tgctgccgta taagttcgag
     1261 gcgggtacgc cgaatgttgc cgacggcatc ggattgggag cggcggtgga ctatttgcgc
     1321 gcgctcgatt ggccggcagt agcagcccac gaagatcgtt tgctgcgata cgcgactgcg
     1381 gcactccagc aaatcgctgc tctgcgcatc attggcacgg ccaaggaaaa agccggcgtg
     1441 ttatcgtttg ttttcgatca cgtgcatgct catgatgtcg ggacgatcct agaccaggag
     1501 ggtgttgcgg ttcgagccgg tcatcattgt gccatgccgg tgatgcagcg cttcggcgtg
     1561 ccggcgaccg cgcgggcatc ttttgcattt tacaacactg tcgaagaaat cgatctgttg
     1621 gtgcgcgcca tcgagcgcgt cttgaaggta tttgactaat gtcggaactc gacgatctct
     1681 accaagaagt catactcgat cacaacaaag cgccacggaa ttttcgcgcc atatccggtg
     1741 cgaaccgccg tgccgaaggt tacaatccgc tttgcggcga tcaggttacc gtctatctcc
     1801 aggtggaaga cggtgtgatc aaggacatca gttttcaggg ttcgggttgc gcgatttcca
     1861 aggcatcggc ctcgatgatg actaccgagc ttaagggtaa gcccgagagt gccgcgcacg
     1921 agctatttga aaacgttcac aacatgctta ccggcgaact agataataaa ggcggcggta
     1981 gcgagcgggt cggcaagctg gcaatcctat cgggagtgtg caagtttccc gcccgggtga
     2041 agtgcgccag tcttgcttgg cacacgatgg aaacggcgct gaaaggcgaa aagcatccgg
     2101 tgcagaccga gtgattcgcg atgctaaata ggacgcgacg ccaatgacaa ctgacaaacc
     2161 aattactctg atacgggatt gttcggcggt gatggtaccg agcggtgaac cgttatcgct
     2221 taaatgcggt agtcaggtgt ggcttactca gtcgttgggc ggcggctata cggtgatgac
     2281 cgatcacggt tacacggtgc gcgtcgacgg ccgcgatggc gatgcactgg gcttcagcga
     2341 tgggcaggcg acgaggggcc aatcgattga aaagaaagga tcgctgcaag agcgagtctg
     2401 gcagcaactg cgttcctgtt tcgatccgga aatcccggtc aacatcgttg acctcggttt
     2461 gatctatgat tgccgggtcg aacctcttcc caacggcggc cataaggcac tggtactatt
     2521 tactctaacc gcacaaggct gcggcatggg tgagtttctc aaggccgata tcagacgcaa
     2581 gttgctcgag gtgagtgacc tcagcgaggc ggccgtcgag ttggtatggg atccgccgtg
     2641 gagccaaagc cgcatgtcgg tcgcggccaa acatcaactc ggtattgaat aggcgcgaaa
     2701 aaatggaaac catcaatcag acgattatcg aagccgaggt gatcgaggca ttgcgcaccg
     2761 tcttcgatcc ggagattccg gtaaacgtct acgaacttgg gttgatttac ttggtcgaag
     2821 tgagtagcga gggcgcggtg atgattcgca tgacgctgac ctcgccgcat tgccccgcgg
     2881 tgcagtcgat gccggctgaa atcgaaagca aggttcggac gatttctggg gtcactgatg
     2941 tgaacatcga tctggtatgg gacccagctt gggatcaatc taaaatgtcg gaagcggcgc
     3001 ggctacaatt gggtatgatg tagcacggtt tggcttcgtt ttctgtcccg cgacgatcta
     3061 ttcgaattca ttatgacccc gacatccttt gaacagttag gcggtgaagc gaagttgcgc
     3121 gcagtgatcg atactttcgt cgaccgggtc tttgccgacc gcatgattgg gttcttcttt
     3181 cgtagcgccg acaaggcgcg cattaaggaa atggagtacc agctggcggc gaattttctc
     3241 ggcgctaaaa tcgaatatgg tgggcgaccc ttggatcagg tgcaccgtaa gttcccgatc
     3301 atgggcggcc actttgcgag acgacggcaa attcttaagg agagcatcga ggctcatggc
     3361 gttcccgacg aaatcaagaa gctctggctc gatcataccg atgcactgcg atcattaatc
     3421 acgcccgaaa acgattcggg atgcgatccg atagctgcgc gagaacgcgt caagactgcg
     3481 taaccctata agttttttgc cttttcccgg actaaacaat tgtcggcgct agaaccttgt
     3541 gctactaata gtggcgggcg ttgaggtagt catctaggac ttgtgtgaga ttagccgagc
     3601 tttacagcca acctaggttg accctatcag tcgagttctt tcctccaaag acagaaaaag
     3661 gcgaggaaaa cctcttcagc gaaattgagc tgatcaagag gataaacccc gccttctgtt
     3721 ctgttacgta cggggcgggc ggaagcacgc gcgaaaaaac cgtcgatttg gtcgatacga
     3781 ttcgctcagg gtgcggtcta gaagtcatgt gtcacctaac ggtggtcggg caatcgaagg
     3841 acgatgcccg cgcggtgcta aagaaacttc aaacttgcgg gatcgagaac ctcctcgctc
     3901 tcggcggtga cccaccccag gggagttccg actggcagcc ccatcccgac ggattccatc
     3961 atgcgatcga actggtccgc gaggccgtgg cgatgcgatc gttttcggtc gcggtggcag
     4021 gctttcccga gatccatcca cgggcgctga gccgggatgc cgatttgcaa tttctcaaag
     4081 agaaagtcgc ggcgggcgcc gatgcgatca tcacgcaact cttttttgat aaccaagatt
     4141 tctatcgatt cagcgaggat ctccgtaagc tcggtgttgt cgtgccgatt attcctggag
     4201 tcttgccgct tcaatcggcg ccgcaagtgc gccgcttcac gtcgctgtgt tgttcaaaga
     4261 tccccgataa gttggagcga gaattaatca aagtcgagaa tgatgaggcc gccgcagttg
     4321 cgctcgggat cgattatgcg acgcagcaat gtgaggaatt gatcaagttc ggtgtgcccg
     4381 gactgcattt ttacagtcta aataaatccc attcggtgtt ggcgattcac aagaatctcg
     4441 gcctgtgatt cttgccgtcg aggcaattcc gagtcgctcc tagcgccttt taatgttcaa
     4501 cgagagcatc atggctgatg atcaacctat tcttactgaa gatgataacc agcaggtcga
     4561 agtgcgcaag caaaagctcg ccaaacttcg cgacggcgcc caggtggttt atccgaacga
     4621 cttcaagccg tctcattctg tgaccgatgt ttttgcccaa gcgagcaatc tcagcgatga
     4681 cgagctgcac gccaattcga ttgctgtcag cgtggccggg cgaatcatgg caatccgccg
     4741 catgggcaaa gcatcattct tccagattca ggatcgacgc ggccggctgc aaatctacgc
     4801 gcgcaaagat aagatcggcg atgatgcata cgagcttttt caaactttgg atattggcga
     4861 tctggtcggt gtctccggcc atgtctttcg caccaaaacc aaagaactga ccgtcgaggc
     4921 cgctggtttg cgactcctcg ccaagtgctt gcgaccgctg ccggagaaat ggcatggatt
     4981 ggccgatgtc gaggcgcgct atcgccagcg ctacgtcgac ctcatggtca accctgaagt
     5041 gcgcgatgtg ttcgaaaagc gctcgcggat cgtgcgcctg ctccggcaat tttttgaagc
     5101 gcgagatttt ctcgaagtcg aaacgccgat gatgcagtcg atccccgggg gcgctgcggc
     5161 gaaacccttc accacgcatc acaatgccct cgacttggaa ttgtatctca gagtcgcgcc
     5221 cgagttgttc ctcaaacgtc tgttggttgg cggctttgag cgggtcttcg aacttaatcg
     5281 aaattttcgc aacgagggaa tttcggtgcg gcataaccca gaattcacca tggttgagtt
     5341 ctatcaagcc tatgcgactt tcgaagactt aattcaactc accgaagagc tgtttgtcgt
     5401 catcgccaat gatattgccg gaggcctgac agtgccttac aacggcgcga cgatcgagct
     5461 gacccggccc tggcagcgcc tgaccattcg cgatgcagtc atggttcacg gcggtgctca
     5521 ggagaaagat gtagcgagcc tggaaagcct gcgaaccttc gctcagcaga gcggtttaaa
     5581 gatcgatctc aatgttcctt acggcaagct cttagtcgcg gtcttcgagg aagtcgcaga
     5641 agcaaaattg attcagccga cattcttaac cggttttcct atcgaggtct caccgttggc
     5701 tcgcaagaat gatcggaatc cggcgatggt cgaccgcttc gagctctata tcggcggccg
     5761 cgagcttgcc aatgcttttt ctgaattaaa cgatcctttt gaccagcgac aacgcttttt
     5821 agaacagatg caagcccgag ctgccggcga tgaagaagct aatccgatcg atgacgatta
     5881 cgttcgcgcc cttgagtacg gcatgccacc ggccgccggc gaagggatcg gcatcgaccg
     5941 cttagtgatg tttttcacca attcaccgtc tatccgggat gtcatcctct ttcccttgct
     6001 gcgcccgcac cgttaatcgc cacattaatc caacagatgc gctacgaact attcatcggg
     6061 ctgcgttact tacaggcgcg ccggcgcgag acttttatct cgctcattac cctaatttcg
     6121 atctgcgggg tgatgatcgg cgtaatgact ttaaacgtcg tgatgtcggt tatgaccggc
     6181 ttcgaagaaa ccttgcgtga tcggttgctc ggcatcaatg cgcatattgc tttggtccga
     6241 tccggcgagc cgatgaggga ctacgagaaa ctagcgcagc aaatgagtaa ggagccggga
     6301 gtggtcgcgg cctcgcccac ggtgtatggc caagtcatgc tgactgcggg ttcgcgggtg
     6361 accggcgtgg tggttcgcgg tgtcgatccg gatcgtgtta atggcgtggt gaatattcag
     6421 agtttcatgc gcgagggcaa cctcgacgga cttaaaaccc agcacccgat ccaggtcgat
     6481 gagcgtacgg taagtctgcc cgggatcatt ctcggcgaga ggcttgccaa ccagctgggg
     6541 gtttttcgcg gcaacgcggt gcaggtggtt tcaccgttgg gcagtcccac ggctattggc
     6601 gtaattccca aggtgcgccg cttcgtcgtt agcggcattc tcaaaagcgg catgagcgaa
     6661 atcgattcaa cactggtgtt catgagtctt gcggacgcgc aaaaattttt tgaactgggt
     6721 gatggtgtca ccaatatcga gatgcgggta caggacgtca gtcgctcccg ggaaatcgcc
     6781 gaccgcattc agcggcagct aggatttccc tattttgccg aagattggac gcgcctgtgg
     6841 cccaatctct tttctgccct caagctggaa aagaccgttt actttcttgt cttgctcttg
     6901 atggtgctca tcggcgcatt caatattgta tcgactctcg tcatggtcgt aatggagaag
     6961 cgtaaggaca ttgcgatcct gcgctcgatg ggtgcgactc agaaaagcat ccggcaaatt
     7021 tttttgctga aaggttgttt gatcggcgtc gtaggaacga tcttgggtgt gatcctc
//
LOCUS       DATLCH010000112         3000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9286002, whole genome shotgun sequence.
ACCESSION   DATLCH010000112 DATLCH010000000
VERSION     DATLCH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9286002"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1733)
                     /locus_tag="VMZ02_03320"
     CDS             complement(156..1733)
                     /locus_tag="VMZ02_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUR70990.1"
                     /translation="MVDSEWDVILIGAGQNNFALGTYLAMAGLRTVICESRLENGGRL
                     ASEEITRPGYWHNTLAYFQDNREVSPVWKELHWDDAHHAEFVSPPVISALLFADGQSI
                     AHHQSIEQTITSIKRFSTQDGEAWRQLHHRYSQLIRDYLIPYYYESPQHGGERLQKLD
                     NEPHGKEFQRLWQLSPRQVVDEVFDKDAVKTLVLSQMTIPRGVGIDYAGGGIEVLKLI
                     AGEEKPELARGGSHSIAQVLQRAYVSNGGQIRAVHHVDKIIINDDGRAVGVRLRDGRE
                     WQARLAVVSNADPYATLVEMVGEDYLPRTFFERVKDIQVDEFSYFQVHLALKAPVRYA
                     VHESNNSAVGQAMNVNMGPERPADLEAMWQELRAGEFPAHACLHAICPTAFDPLQAPA
                     GKHAASVYLPVPYQLKGKRPEDWVKLKESFLHTVLQAWRRYATNLTDDNITMKIAMEP
                     HYIAGRWPNMRRGSVWVARKIPTQMGEMRPIEQISDYRTPIESLYQVGVAMHPADAVI
                     AGSGYNAWRVIKHDLGL"
     gene            complement(1784..2803)
                     /locus_tag="VMZ02_03325"
     CDS             complement(1784..2803)
                     /locus_tag="VMZ02_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR70991.1"
                     /translation="MSHGVLLGIVVMLVSMIGGLTAGFAQDKQLIDAAKKEGGKVVVY
                     GSLENDTMDLLAAAFKKKTGLDVDYWRDSANKVTDRVSAESRAGKPQADVVLTTTSTL
                     RLIQKDGLLAKYDSPAAKAFPQTVIDPNLGPAYRSTVIGVVYNTGIIKPGEAPKSVED
                     LVKPQYKGKVVFPDPSQHTTTAQWLASLHKIIGQERAEKFIHDLAASKPLLVSSLTPA
                     GERITTGETPIGVAFLKNVVFYGRNGVPLDYVRSGKFMGDGQSITMANKAPHVNAGKA
                     FIDYFLGEESLKIMANIGEFVTRKGIYPPLPDANKIQLVDMIDMDKDAFAHKMAEYKK
                     IFLQN"
BASE COUNT          634 a          851 c          798 g          717 t
ORIGIN      
        1 gtggccggcg ccgattacga tgccgtcgta ttctaggagt tttgccataa catccgatct
       61 gaaaactatt tgaacgtttt gaacgatttg aacggcttca acgtcgcgtg gttcaaatcg
      121 ttcaagtagt tcaagccgtt caatatgttc gccccctata acccgagatc atgcttgatc
      181 acccgccacg cgttgtaccc cgagccggca atcaccgcgt cggctggatg catcgcgaca
      241 ccgacttgat acaaactctc gatgggcgtg cggtagtcgg agatttgctc gatggggcgc
      301 atctcgccca tctgtgtcgg gatcttgcgc gctacccaga ccgagccgcg gcgcatgttg
      361 ggccagcgtc cggctatata gtgcggttcc atggcgatct tcatcgtgat gttgtcgtcg
      421 gtcaagttgg ttgcgtagcg gcgccaggcc tgcagcactg tatggagaaa gctctccttg
      481 agcttcaccc agtcctccgg ccgtttgcct ttcagttgat acggcacggg caggtagacc
      541 gatgctgcat gtttacccgc gggcgcttgt aatgggtcga aggcggttgg acagatggca
      601 tgcaggcaag cgtgagctgg gaattcgccg gcgcgaagct cttgccacat cgcttcgaga
      661 tcggccggtc tttcgggtcc catgttgacg ttcatcgctt ggcccaccgc cgagttgttc
      721 gattcatgca cggcataacg aaccggcgct ttcaacgcca gatggacttg gaaatacgag
      781 aactcgtcga cctgaatatc cttgacgcgc tcgaagaacg ttcgcggcag ataatcctcg
      841 ccgaccattt ccaccagcgt cgcgtacgga tcggcgttgg ataccaccgc gaggcgcgcc
      901 tgccactctc gaccgtcgcg caagcgcaca cccacggccc ggccgtcgtc attgataata
      961 atcttgtcga catgatgtac ggcgcgaatt tgtccgccgt tggagacata ggcgcgctgt
     1021 aatacctgcg cgatggaatg cgaaccgccg cgcgccagct cgggtttttc ctcgccggca
     1081 atgagtttca atacctcgat gccgccgccg gcgtaatcga tcccgacgcc gcgtggaatg
     1141 gtcatctgcg acagcaccaa cgtcttcacc gcatccttgt caaatacctc gtcgaccact
     1201 tgccgcggcg acagctgcca caatcgttga aactccttgc cgtgcggctc gttatcgagc
     1261 ttttgcagtc tctccccacc gtgctgcggc gactcgtagt aatacggaat caagtaatcg
     1321 cgaatgagtt ggctatacct gtgatggagc tggcgccacg cctcgccatc ttgggtggaa
     1381 aagcgcttga tcgaggtaat cgtctgttcg attgattgat ggtgcgcgat ggattgcccg
     1441 tcggcaaaca acagagcgct aatgaccggt ggtgaaacaa actcggcgtg gtgggcgtcg
     1501 tcccaatgca gttctttcca tactggcgag acttcacgat tgtcttggaa ataagccagg
     1561 gtgttatgcc aatagcccgg ccgggtaatc tcttcactgg caagccgccc gccgttttcc
     1621 aaccggctct cgcaaataac cgtcctgaga ccggccattg cgagataggt gccgagagca
     1681 aagttatttt gtccggcacc gatgagaatc acgtcccact cactgtcgac cacgtattcc
     1741 tccactcgat gtgaatgata ggtacggcta gatgtttgaa ccgttagttc tgtaagaaaa
     1801 tctttttgta ctcggccatc ttatgcgcaa acgcgtcttt atccatatcg atcatatcga
     1861 ccagttgaat cttgttggca tcgggcaagg gcggataaat acctttgcgg gtgacgaact
     1921 caccgatgtt ggccatgatc ttgaggcttt cctcgccgag aaaataatcg atgaaagctt
     1981 tgccggcgtt cacatggggc gctttattgg ccatggtgat tgactggccg tcacccatga
     2041 atttccccga gcgaacgtaa tccagcggca cgccattacg accgtagaag accacgtttt
     2101 tcaaaaatgc cacgccgatc ggcgtttcac cggtagtgat gcgctcgccg gccggtgtga
     2161 gcgacgatac cagaagtggt ttcgaggccg ccaagtcgtg aatgaacttt tcggcgcgct
     2221 cctggccaat tatcttatgc aaacttgcga gccattgggc cgtcgtggtg tgctggctgg
     2281 gatcggggaa aaccaccttg cccttgtatt gcggtttgac taaatcttcg accgacttgg
     2341 gcgcttctcc cggtttgatg atgccggtat tgtaaaccac cccgatgacc gtgctgcgat
     2401 aggccggccc gagattagga tcaatgacgg tttggggaaa cgccttggcc gccggcgaat
     2461 catatttcgc cagcaacccg tctttttgga tcaagcgcaa agtagaagtc gtcgtcaaca
     2521 ccacgtcggc ttgcggtttg cccgcgcgcg attcagcgct cacccggtcg gtcaccttgt
     2581 ttgccgaatc gcgccaatag tcgacgtcta gaccggtttt cttcttaaac gccgcagcca
     2641 gtaaatccat ggtgtcgttt tccagcgagc cgtaaacgac gaccttgccg ccttcttttt
     2701 ttgccgcgtc gatcaattgc ttatcctggg caaatccagc tgtcagtcct ccgatcatcg
     2761 aaacgagcat aacaactatt cccagcagaa ctccgtgcga cattaaatcc tccgttcaat
     2821 ccaatgaatt aactgaaaac ctcgagcgcc tcgagccgac cgatgtttgt ccttgggcga
     2881 cgcctttgct aacgtacttt tacaattatc gcttatgacc gcacaagaaa agctaaaaaa
     2941 aatccgcgaa gagttaaaac aaactttcct cgaacgcggc gacttgatcg acggcgcgct
//
LOCUS       DATLCH010000113         4193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7624326, whole genome shotgun sequence.
ACCESSION   DATLCH010000113 DATLCH010000000
VERSION     DATLCH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7624326"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VMZ02_03330"
     CDS             <1..546
                     /locus_tag="VMZ02_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70992.1"
                     /translation="SRFIQQDLNAGLPDLVFSDYENVLLLDVIEHLMSPETFLDRLRE
                     SMKYCTATKIIVTTPNVGFIIIRLMLLLGQFNYGKRGILDLTHKRLFTFATIGHLFEQ
                     RGFKVSTCRGIPAPFPVALGTGFLSRLLLAVNKFLIRLSPSLFSYQILVVAEPRPSLE
                     ILLQRALDESATRAQALATKS"
     gene            714..2099
                     /locus_tag="VMZ02_03335"
     CDS             714..2099
                     /locus_tag="VMZ02_03335"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HUR70993.1"
                     /translation="MVEMEKIVSLCKRRGFIFQSSEIYGGTGSCWDYGPLGVELKNNV
                     KRAWWRDCVQNRGDMVGLDASILMHPRVWKASGHLDHFTDPMIDCKVCKGRFRADKLE
                     DARCPQKPSKQPGQHDACALTEARMFNLMFKTFMGPVEDDAAVAYLRPETAQGIFVNF
                     ENVQQSMRLKLPFGIAQIGKSFRNEITPGNFTFRTREFEQMEIEFFVMPGTDEEWHQR
                     WLDERFAWYPKYGIRKENLRLREHEKDELAHYAKRTADIEYLFPMGWSELEGVANRTD
                     YDLKQHAQFSGKTLDYFDEESKQKIVPFVIEPSAGADRGTLAFLVDAYREEEVKGEKR
                     VVLNFHRELAPIKVAVLPLLKKNPRIVETAHKLCADLRKRWHSVYDDTASIGRLYRRQ
                     DEVGTPFCVTVDVQTVGDEKSAADDQVTIRDRDNMQQIRVPLASLTAVMEKLMDGEWA
                     QIHGQHGLARN"
     gene            2298..4016
                     /locus_tag="VMZ02_03340"
     CDS             2298..4016
                     /locus_tag="VMZ02_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR70994.1"
                     /translation="MLGVVSLIATDLAGIALPWLIKGGIDTALRAKEGTGALLAYPLL
                     IVGAAALQGFFRFCWRINIHGFSRRCEADLRDVVFAHLQKLPLSYFQYHKIGDLMSRL
                     TNDIHAVRELMGFGSLAIVDALVVIVFSLTLMTAIDPWLTLWSMIAMPLIPLVVRLFG
                     RLIFRWSRATQDQVSDMSAYALENFSGIRVVQAYAQEENQISGFNRISSEYRERTMWL
                     ATLWGIFWPLMQVHAGIAATIVLWLGGRQVLAGTMSLGEFVAFNGYLAMLTWPLMAIG
                     YTANQYQRGTAALTRIAEVLDMPVGARYANGASCEPVQTVRGDIELRNLTFSYGADRA
                     AELRNIDLHIPAGKSCGIVGETGSGKSTLVNLMLRLYEPPENSILIDGRDIRRMPLRQ
                     LEDAVGYVAQDIFLFSGSLRENILLGIEHGELARVEEAAKIARLLPAIESFSAGFDTV
                     IGERGVRLSGGQKQRTALARALIKNPPILILDDAFSSVDVETEEEILRELRQFMRGRT
                     TLLISHRISTVRDADTIVYLRDGAIIERGSHEQLLARRGAYFSLYQRQSLERQVASLV
                     LQDGQG"
     gene            4013..>4193
                     /locus_tag="VMZ02_03345"
     CDS             4013..>4193
                     /locus_tag="VMZ02_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR70995.1"
                     /translation="MIGAQLPDEEILGKAYDARLMMRILRYLRPYWKLLAVAFGFLLL
                     QTGTQLLGPYITKIAI"
BASE COUNT          868 a         1181 c         1179 g          965 t
ORIGIN      
        1 agccgattta tccagcaaga cttgaatgct gggctgccgg acctggtgtt ctcagactat
       61 gagaatgtcc tgctgctcga cgttatcgag cacttgatgt cacccgaaac gtttctggat
      121 cgcttgcgcg aaagcatgaa gtactgcacc gctaccaaaa tcattgttac cacccccaat
      181 gtcggcttta taatcatccg cttgatgctg ctactcggcc agttcaatta tggaaagcgc
      241 ggcatcctcg atcttaccca caaacggcta tttacctttg caacgattgg tcacctgttc
      301 gaacaacgcg gcttcaaggt ctccacctgc cgcggcattc cagccccgtt tccagtggca
      361 ctgggaacgg gttttcttag tcgtttgttg ctagccgtaa acaaatttct tattagactc
      421 tcgccctcac tgttttccta tcaaatactc gtggtagctg aaccaagacc ttcattggag
      481 atactcttgc agcgcgccct ggatgagtcg gccacccgtg ctcaggcctt ggctacgaaa
      541 tcataaccct ctgccactcg tttaacttcg tttgctcgat gcttgttggc tttgcgccaa
      601 ttcacggtcg ctgcgcgttg atctgttgct ccgtgccgaa aaaatttcta gttttgactc
      661 tcccaggcgt atccattaca ttgttaggct tctgaactag acaggaaatt gtaatggtcg
      721 agatggaaaa aattgtgtcg ttgtgtaaac ggcgcgggtt tatttttcaa tccagtgaga
      781 tttatggcgg caccgggtct tgttgggact acggaccgtt aggggtcgag ctcaagaaca
      841 acgtcaaacg cgcctggtgg cgcgactgcg tgcagaaccg cggcgatatg gtcgggctcg
      901 atgcgtcgat tctcatgcac ccgcgggtct ggaaggcgag cggacatttg gatcacttca
      961 ctgatccgat gatcgactgc aaagtttgca aggggcgttt tcgcgccgac aaactcgaag
     1021 acgcgcgctg cccgcaaaaa ccgtcgaagc agcctggcca gcacgacgct tgcgcgctca
     1081 ccgaagcgcg catgttcaat ttgatgttca aaactttcat ggggccggtg gaggatgatg
     1141 ccgcggtcgc ttatctgcgc ccggaaaccg cccaggggat tttcgtcaac ttcgagaacg
     1201 tccagcagtc gatgcgcctg aagctgccct ttggcatcgc ccagatcggc aagtcttttc
     1261 gcaacgagat cacgcccggc aacttcactt tccgcacgcg agaattcgag caaatggaga
     1321 tcgaattctt tgtgatgccg ggcacagatg aggagtggca ccagcgctgg ctcgacgagc
     1381 gcttcgcctg gtatcccaaa tacggtattc gcaaggaaaa cctccgattg cgcgagcatg
     1441 agaaagacga gttggctcac tacgccaaac gcaccgctga catcgagtat ctgttcccga
     1501 tgggttggag tgaactcgaa ggagtcgcca atcgcaccga ttacgatttg aagcagcatg
     1561 ctcaattcag cggcaagacg ctcgattatt tcgacgagga gagcaagcaa aaaatagttc
     1621 ccttcgtcat cgaaccctcg gccggcgctg accgcggcac tttggcgttt ctcgtcgacg
     1681 cctatcgtga ggaggaagtg aaaggcgaga aacgcgtggt cctaaacttt catcgcgagc
     1741 tcgcgcccat caaagtagcc gtcctaccgc tgttgaaaaa aaatccgcgc atcgtcgaga
     1801 ccgcgcataa gctttgcgcc gatctgcgca agcgctggca ctcggtttac gacgacactg
     1861 cgtccatagg cagactctat cgccgtcaag acgaagtcgg cacgccgttt tgtgttaccg
     1921 tcgacgtcca gactgttggc gatgaaaagt ctgcggcaga cgatcaagtg acgattcgcg
     1981 accgcgacaa catgcagcag attcgcgtgc cgctggcgtc tcttaccgct gtcatggaaa
     2041 aactcatgga cggtgagtgg gcgcagattc atggccaaca tggccttgcg cgtaattgaa
     2101 accgaacgcg gcgcggccaa accacagaca agaatggccg cacagaacgc aaaggccgca
     2161 aaagaagtgg ggatttgtca gggcgggtct gaaacccgcc ctcgtcgttt gcactcagct
     2221 ccgaaccaaa ggatagagag attcttcgtt attattaccg ggtcaaattt cttattgacc
     2281 gttaccgccg gctactcctg ctcggcgtcg tttccctgat cgccaccgat ctcgccggca
     2341 tcgccttgcc ctggttgatc aagggcggca tcgacaccgc tttgcgagcc aaggagggca
     2401 ccggcgcgct actcgcctac ccgctgctga ttgtcggcgc ggccgcactg caaggtttct
     2461 ttcgcttctg ttggcgcatc aacattcatg gtttctcgcg ccgctgcgag gccgatctgc
     2521 gcgatgtcgt gttcgcccat ctgcaaaagc tgccgctgag ctattttcaa taccacaaaa
     2581 ttggcgatct gatgtcgcgc ctgaccaacg atatccatgc tgtgcgcgag ctcatgggtt
     2641 tcggctcgtt ggcaattgtc gatgccctgg tggtgattgt tttcagcctg acgctgatga
     2701 ccgccatcga tccttggttg accttgtggt cgatgatagc gatgccattg atacccctgg
     2761 tggtgcgctt attcggccgg cttatttttc gttggtcgcg cgcgacccag gatcaagtga
     2821 gcgacatgag cgcctacgcg ctggagaatt tttccggcat ccgcgtcgtt caagcctacg
     2881 cgcaagaaga aaaccaaatc agcggtttca atcggattag ctcggagtac cgggaaagga
     2941 ccatgtggct ggcgacgctg tggggcatat tttggccgct catgcaggtc cacgccggca
     3001 tcgccgcaac catcgtgctc tggctcggcg gtcggcaagt attggcgggc accatgagcc
     3061 tgggtgaatt cgtcgctttc aacggttatt tggcgatgct cacctggccg ctcatggcca
     3121 tcgggtacac agccaatcaa tatcaacgcg gcaccgcggc gttgacgcgc atcgccgaag
     3181 tgctcgatat gccggtcggt gctcgctatg ccaatggcgc ctcttgcgag ccggtacaga
     3241 ccgtgcgcgg ggatatcgag ctgcgcaatt tgactttctc ctatggcgcc gatcgggcgg
     3301 ctgaactgcg caatattgat ttgcatattc ccgccggcaa gagttgcggc atcgtcggcg
     3361 agaccggctc gggcaagagc acgctggtga atctcatgct gcggctttat gagccgcccg
     3421 aaaattcgat tctgatcgac ggccgcgata ttcgccgcat gccgttacgc cagctggagg
     3481 acgccgtcgg ctacgtcgcc caggatatct ttctattctc cggatcgtta cgggagaata
     3541 ttttgctcgg catcgagcac ggcgaattgg cgcgggtcga agaggcggcg aaaatcgccc
     3601 ggctgttgcc ggcgatcgaa tctttcagtg ccggcttcga taccgtgatc ggcgagcgcg
     3661 gggtgcgcct ctcgggcgga cagaagcagc gcactgcgtt ggcgcgggcg ctaatcaaaa
     3721 atccgccgat tctgattctc gacgacgcct tttccagcgt cgatgtcgag accgaggaag
     3781 agattcttcg cgagctcaga caattcatgc gcggccgcac gactctgttg atttcgcacc
     3841 ggatttcgac ggtacgcgac gccgacacga tcgtatattt gcgtgacggc gcgattatcg
     3901 agcggggcag ccacgagcaa cttttggcgc gccgtggcgc ttatttcagt ctctaccagc
     3961 gtcagagttt agaacggcaa gtcgcgagct tggtgcttca ggacgggcag ggatgatcgg
     4021 cgcgcaactt cccgacgagg aaattctcgg caaggcctac gatgcgcgct tgatgatgcg
     4081 catcctgcgt tacctgcggc cctactggaa gctgctcgcc gtggcgtttg gctttttgct
     4141 gctgcaaacc ggcacgcaat tactcgggcc ctatatcacc aagattgcca ttg
//
LOCUS       DATLCH010000114         3562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2589539, whole genome shotgun sequence.
ACCESSION   DATLCH010000114 DATLCH010000000
VERSION     DATLCH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2589539"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VMZ02_03350"
     CDS             complement(<1..1427)
                     /locus_tag="VMZ02_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HUR70996.1"
                     /translation="MGQRAGRMHKLAAACLLATLTLLATTSAAFAQDQARRLVALLDY
                     LGGDYQNAVQNGKIVNKDEYAEMQEFSKRITELMGQLKAIEKADKAQIEPMLKSLVGE
                     VEKKGDEKTVAKLANDAKERLIATYKIVPHPRQLPALADGRTIFIENCVQCHGPGGKG
                     DGPGRESMNPKTPLPANLTDPEFMAGLSPFKAYNTVSFGIENTAMSSFAALSDEQRWQ
                     VAFYALALRFPAETSAAGEAWLKGKSLPPDLVNVATLATTSDGDLSAKIKKLFPDGPN
                     GAILAYLRRGLLDKKSADPLLIARTLIGEAATLYAGGENEKAYQKAIEAYLDGFEMAE
                     PALFAKDGSLGRSLENQFTQFRNSIRQRVPVEEIQKQRREIDLKLDQAGLTLASGDEN
                     LSGFYAFLNSALIILREGLEATLILAAIIAMLKVMGAATAIRYIHLGWVLALIAGAVT
                     WIAAQTVLTFSGQHRESMEGFISIFA"
     gene            complement(1491..2258)
                     /gene="phnE"
                     /locus_tag="VMZ02_03355"
     CDS             complement(1491..2258)
                     /gene="phnE"
                     /locus_tag="VMZ02_03355"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HUR70997.1"
                     /translation="MRATLFLPFATTAFLIATLAHLGFFDLARLSRGIRNLGILGAEL
                     VPPDMGILSTAGAALWETVEMSFAGTLLGFVASLPLSILGTRRLFSSWVSGLARFIAA
                     GIRTIPVLLWAIVFVILLGLGPLAGTFGIAAYTVGYLAKIYADLFEGTDPEIIEAVQS
                     TGASKWQLVRFVILPEAANAVLAQVLFMLEYNIRASSVLGFVGAGGLGFVIQIYLQSL
                     DYRRLATVLLLVLVVVLAMDGISAWLRNRFLLSAQAT"
     gene            complement(2255..3004)
                     /locus_tag="VMZ02_03360"
     CDS             complement(2255..3004)
                     /locus_tag="VMZ02_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR70998.1"
                     /translation="MTQQAGEPYALQADRVWYSYRNRDWILRAIALAVPQGQHTVIMG
                     PSGTGKTTLLRVLAGILQPSDGSVSVFGRPIKQLSRRQLSALIGYIPQQLGLVRNLSA
                     IDNVLMGALGRISDSQALLGLFPRGEIEKAEAALDMMGIAHKRSAQVFHLSGGERQRV
                     AIARTLLQHPRVVIADEFASDLDLALASEILDRIRRAADRENITFIMSMHRIGLARQF
                     GDDVLALKNGEINPGFIGNEVLEDARTEVAL"
     gene            complement(3001..>3562)
                     /locus_tag="VMZ02_03365"
     CDS             complement(3001..>3562)
                     /locus_tag="VMZ02_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUR70999.1"
                     /translation="YYSYWIVSPDAPYKDLPSLKGKSACFPSPISTSGFVAPIGRMVE
                     MNLLQPGEGKEADPKAFFKEVLFGGGYGQCWQALKQGQVDATVIAGDVPEKLYNEVLA
                     GTRTIEQQGPIPSHAVVLSKELKDPLRARAVQSIVKLGAPEHRELMRTFISSIFVGFK
                     PTTASEHLGSLQKFLKLANLKYTERL"
BASE COUNT          788 a          969 c         1053 g          752 t
ORIGIN      
        1 gcgaagatcg agatgaagcc ttccatgctt tcgcggtgct ggccgctgaa agtcagcacg
       61 gtttgggcgg caatccaggt aacggcgccg gcgatcaagg caagaaccca acccaaatga
      121 atataacgga tggcggttgc cgcgcccatg actttcaaca tggcgatgat cgcagcgagt
      181 atcagcgtcg cttccaatcc ttcacgcaaa ataatcagcg cggaatttag gaaggcataa
      241 aaaccggaca aattctcgtc gccgctggcc agcgtaagac cggcttggtc cagcttaagg
      301 tcgatctccc ggcgctgttt ctgaatttct tcaacgggaa ctcgttgcct gatcgagtta
      361 cggaattgag tgaattggtt ttcgagggac cgccctaaac tcccgtcctt ggcgaacaat
      421 gccggctcag ccatctcgaa gccatcgagg tacgcctcta ttgctttttg atacgccttc
      481 tcgttttccc cgccggcata gagcgtggcg gcttcaccga tcagggtgcg tgcgatcaag
      541 agcggatcgg cggatttttt gtccaggaga ccgcgtcgaa gataggcgag aatcgctccg
      601 ttaggtccgt ctgggaaaag tttttttatc ttagcggaaa ggtcaccgtc cgacgtggtc
      661 gccagagttg ccacattgac caaatccgga ggcagcgact tgcctttcaa ccaggcctct
      721 ccggcagcgg acgtttcagc gggaaagcgc agcgccaaag cataaaacgc tacttgccag
      781 cgctgctcat cggaaagcgc ggcaaacgat gacattgcgg tgttttcaat tccgaagctg
      841 acggtgttgt atgccttgaa aggcgataac ccggccatga attcggggtc ggtcagattt
      901 gccggcagcg gcgtcttggg attcatagat tctcggccgg ggccgtcgcc cttacccccg
      961 gggccgtgac attgaacgca attttcaatg aaaatcgtcc gcccgtcggc caaagccggg
     1021 agttggcgcg gatgtgggac gatcttgtag gtggcgatca gtctttcctt ggcatcgttg
     1081 gcgagtttcg caacggtttt ttcatcgccc tttttttcga cctcaccgac aagggacttg
     1141 agcatgggtt cgatttgagc tttgtcggct ttttcgatcg ctttgagctg ccccatgagt
     1201 tctgtgatgc gcttggagaa ttcctgcatc tcggcgtatt cgtctttgtt gactattttg
     1261 ccgttctgga ccgcgttttg ataatcgcct cctaggtaat cgagaagggc gaccaggcgc
     1321 cgcgcctgat cttgagcgaa cgctgcagag gtcgtagcca acagagttaa cgtcgctagt
     1381 aagcaagccg cggcaagctt gtgcatgcgt ccggctcttt gacccactcg cttggaccgc
     1441 cggttagccg aggattcaga gatcgttcga ggtatttggg tttccatagg ttacgttgct
     1501 tgcgccgaca gtagaaaacg atttctcagc caagcgctga tgccgtccat ggcgagcacg
     1561 acgaccagga ccaacaacaa gaccgttgcc aatcgccgat aatccaaact ctgcaagtaa
     1621 atttgaatca cgaagccgag gccgccggcg ccgacgaagc cgagcaccga tgaggcacgg
     1681 atgttgtatt cgagcatgaa taaaacttgg gcgagcacgg cgttggcggc ttcggggaga
     1741 atcacgaacc gcaccaactg ccatttcgaa gcgccggtgc tttgcaccgc ttcgatgatt
     1801 tccgggtcgg tgccttcgaa caaatcagcg tagatttttg ccaggtagcc gacagtgtag
     1861 gcggcgatgc cgaaggtgcc ggcaagcggc ccgagaccaa gcagtatgac gaagacgatc
     1921 gcccaaagca gcaccgggat agtgcgaatg ccggcggcga tgaagcgggc caagccggat
     1981 acccaggatg agaacaaacg gcgagtgccg aggatgctga gcggcagcga ggcgacaaag
     2041 ccgagcaagg tgccggcgaa tgacatttcg acagtttccc acaaggccgc gccggcagtg
     2101 gaaagaatgc ccatgtccgg cggtaccaat tcggcgccga ggatgccgag gttacggatc
     2161 ccgcgggaaa gccgcgctag atcaaaaaaa cccagatgcg cgagagtcgc aattaaaaaa
     2221 gcggtcgtgg caaacggcaa aaacagcgtc gctctcacag agcgacctca gttctagcat
     2281 cctcaaggac ctcgttgccg atgaatcccg gattgatttc gccgtttttg agcgcgagca
     2341 cgtcgtcgcc aaactggcgc gccaagccga tgcgatgcat gctcatgatg aaagtaatat
     2401 tctcccggtc cgcggccctg cgaatgcggt caagaatttc cgacgccagc gcgagatcga
     2461 gatcggaagc aaattcatcg gcgatcacga ctctgggatg ctgcagcaga gtacgcgcga
     2521 tggcgacgcg ctggcgctcg ccaccgctca ggtgaaagac ctgagccgag cgcttatggg
     2581 ctatgcccat catgtcgaga gcggcctcgg ctttttcaat ctcaccgcga gggaacaaac
     2641 cgagcaaagc ctgactatcg ctgatgcggc cgagcgcgcc catcaacaca ttgtcgatgg
     2701 ctgacaggtt cctaactaaa ccgagttgct gggggatgta accgatcagg gcgctcaact
     2761 gccgccggct gagttgcttg atcggccggc cgaaaacgct tacggagccg tcgctcggtt
     2821 ggagaattcc cgccaagaca cgtaaaagcg tggttttacc cgtgcccgag gggcccatga
     2881 tgacagtgtg ttgtccctgt ggaaccgcca gtgcgatagc gcgcagtatc cagtcgcgat
     2941 tacgatagga ataccaaact cggtcggctt gcagcgcgta gggctcgccg gcctgctgcg
     3001 tcataagcgc tccgtgtact taagattcgc gagcttgagg aacttctgta aggagccgag
     3061 atgttcgctc gcagtggtcg gcttgaaacc gacgaaaatg ctcgaaataa acgtgcgcat
     3121 cagttcgcgg tgctccggcg cgccgagttt gacaatcgat tgcacggcgc gggcgcgcag
     3181 tggatctttc agttccttgc ttaaaaccac cgcgtgcgac ggaatcggac cctgctgctc
     3241 aatggtgcgt gtgccagcga gcacttcgtt ataaagcttt tcggggacat cgccggcgat
     3301 aactgtggca tcgacttggc cctgcttgag cgcttgccaa cattggccgt agccgccgcc
     3361 gaacagcact tctttaaaaa atgctttggg atcggcttcc ttgccttcgc cgggttggag
     3421 cagattcatt tcaaccatgc ggccgatggg cgcgacgaag ccggaggttg aaatcgggct
     3481 ggggaagcac gccgacttgc ctttaagtga tggcaagtct ttataggggg cgtcgggact
     3541 aactatccag tacgagtaat ag
//
LOCUS       DATLCH010000115         7207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6144191, whole genome shotgun sequence.
ACCESSION   DATLCH010000115 DATLCH010000000
VERSION     DATLCH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6144191"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VMZ02_03370"
     CDS             <1..225
                     /locus_tag="VMZ02_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71000.1"
                     /translation="SSLTALGACHKFTGDRVETYEVPMQPRNLATWFFLAAINLLGGT
                     AAKVLALNNRSSIPPSQLSQATHLRGRINT"
     gene            418..627
                     /locus_tag="VMZ02_03375"
     CDS             418..627
                     /locus_tag="VMZ02_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71001.1"
                     /translation="MESAAAKRSKIISFEEKKFRAVLCDTCGAKMFPESLLQRHLETH
                     RLHQRWISGELRKLQNTFSRMRDIA"
     gene            complement(814..1785)
                     /locus_tag="VMZ02_03380"
     CDS             complement(814..1785)
                     /locus_tag="VMZ02_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HUR71002.1"
                     /translation="MSFGKKLDLLGLYARSLVRIRKNSAFFRDFQTYCMFVGYPRTGH
                     SLIGSLLDAHPNIVIAHELDALRLVKHGFTRRQVFNLLLENSRKMAVSGRSHSGYSYD
                     VPNQWQGRFEQLQVLGDKKGATSAMRFYREPRLLASLRTKIGGELKFVHVIRNPYDVL
                     ATMARRSPHKTLAMHIDVFFRLCSSIESLKTRMDPNEIFDVRLESFIDDPQPYLKQLC
                     SFLGVRAAAGYLNDCASIVFKSPKKSRFDIEWDRESVVKVRERMAAFSFLSGYSYDDG
                     PAMSVVPATPAAPEERAAALNYRSLTGKLLVTIAYSMNDCVLQNLPI"
     gene            complement(1822..2259)
                     /locus_tag="VMZ02_03385"
     CDS             complement(1822..2259)
                     /locus_tag="VMZ02_03385"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR71003.1"
                     /translation="MDPKAQVVQLKTQLVREGHTRFMLAETDLMTLRIHCYAPGIGEN
                     ALHAHTKEDHIFVVLEGTAQFNTERDGQTVLTATKNHAVVLPAGSYYQFCNSGQTPLV
                     MARIGAGQDKADQRLNPDGSAIPGRTHEQGAAKPVLIEGAYFE"
     gene            2439..3308
                     /locus_tag="VMZ02_03390"
     CDS             2439..3308
                     /locus_tag="VMZ02_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUR71004.1"
                     /translation="MTPLFAEIVAIFSAMGWAIDSVLVRFGLRQSNIFAAMLMSYGVS
                     ITCVWSYLIATTSLEFLSSPVMLYYVISGCMQPIFARALFYQGITRIGVARAGPLRGI
                     EPLFAAAIALLVFNEQPGLQVYIGTVLIVTSLWLISGKQEQNNPWRYIDALLPISAAL
                     ISAISQTLRKQALQIIPDPFIAVAMVTSVSLFLLLGFVYASGRAQQLRMNRSSFKFFL
                     GAALVATTAQIFNFIALGRGQMSVIIPLLNTTPLFTVFFSALFLRKIETVNLRIILGA
                     ALMVAGVVCITSR"
     gene            3371..5314
                     /locus_tag="VMZ02_03395"
     CDS             3371..5314
                     /locus_tag="VMZ02_03395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HUR71005.1"
                     /translation="MEIDNEPPVERIDEQAALRMILEGTASETGERFFAALVRNLSKA
                     LNTTGAWVTEFLEAERKLRALAFWFDGAWIPDYVTDLRGTPCEQVIVSAELVHFPENL
                     IELFPEDQDVKTLGAVSYLGMPLKDIDGRILGHLAVIDRRFLPNDPRILALFRIFADR
                     ASAELRRLRAEKQVDEREAKLRRLVGSAMDAIVEFDNQLSVTLLNPAAEQVFQRQSAA
                     IGGEKISAFFSQESGDKLLSLVRALECQPKDRQSMWIAGGLTGCRKDRSEFPAEASLS
                     RSELGENIFYTLILRNLTNRLEAERKILVLSAEAEQLRAEIQSLQGSGDIIGGSAALK
                     NVLRDIEQVAETDATVLLLGETGTGKELVARAIHKASRRADKALITINCAAIPTTLME
                     SEFFGHERGAFTGATQKREGRFALANGGTIFLDEIAELNLELQAKLLRILQEGEFEPV
                     GSSQTRKVNVRVIAATNRDLGQAVRTGNFRDDLYYRLNVFPISLPALRERGDDVIELA
                     NLFANKFAQRSGRRVEPLTDTGKQRLKKYSWPGNVRELQNVIERAVITSQDGRLNLDR
                     ALPGVTEDQPTIGENRSSESQSAERILQVREIQQLERQNILRAMEKAGWRVAGSDGAA
                     ELLGINPSTLNSRIKALGIQRLR"
     gene            5592..6503
                     /locus_tag="VMZ02_03400"
     CDS             5592..6503
                     /locus_tag="VMZ02_03400"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HUR71006.1"
                     /translation="MEHNYSYQSALATSERIGWRIDDIIGPDKPLNFAKPFMPEALAQ
                     VRRLDFLSAGEQRTLNQIRGHEYLAMFGLVEEFILPYVVEHTRTKLSGDDYRVRALLQ
                     FAGEEAKHIHLFKRFRQQFDAGFGTPCEFIGPAEEIKRFVLSHSPLGAGIAILHIEWI
                     TLRHYIEGVRDDQDLDPQFKSLLKNHWLEESQHTKLDTLMIEEIAAAASQSEIELAIA
                     EYADIGKFLDNGLKQQAEFNVDSFVRATRRNLTKSQREQATDNVLKAMRWTYLGTGMT
                     HPNFLATVEKINPAARKQIEEMAPAFC"
     gene            6546..7118
                     /locus_tag="VMZ02_03405"
     CDS             6546..7118
                     /locus_tag="VMZ02_03405"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUR71007.1"
                     /translation="MSASAATKIATVNGLDVDALRQVVDEVKKDPAKGIVEFKVKTGW
                     KGRTRSETTVEGYKLGGEKIKRRFTISADEPFELLGENTSANPQELLMAALNACITVG
                     YVAGAAVNGITLTKLEIETSGQLDLRGFLGLDANVKPGYESLQSVVYIAGNGTAEQFQ
                     QIHDNVMKTSPNFFNISRPVRIDATLKVEN"
BASE COUNT         1652 a         2064 c         1908 g         1583 t
ORIGIN      
        1 agttcgttga cggctcttgg tgcctgccat aaattcaccg gcgatagagt tgaaacatac
       61 gaggtgccga tgcagccccg caacttggca acttggttct tccttgccgc gatcaatcta
      121 cttggtggta cggcagcaaa agttttggca ttaaacaacc gcagtagcat tcctccgtca
      181 cagctcagcc aggcaacgca ccttcgtggt agaatcaaca cctagcaatt ggcgactgtg
      241 cctctttcct tgtcagcgac ggcacgatgt tgacagcagg atgtcaaaca ctcgtgtaat
      301 cgatcgcgcc tcagagtaaa acttcaaaat aattgctgtc ttggccggct cactaaaatg
      361 agggttggtg gcataaccct tgctcaccaa tcatccagct taatcaggag attcgaaatg
      421 gaatcagcgg ccgccaaacg ctccaagata atttcttttg aagaaaaaaa attccgcgcg
      481 gtcctgtgcg acacctgcgg cgctaagatg ttcccagaat cgctgctgca gcgacacctg
      541 gaaacccatc gtctacacca gcgctggatc agcggtgaac tgcgcaagct gcaaaatacc
      601 ttctcgcgca tgcgcgacat cgcctgagcg gttctgcgag ctttccaagg atgggaagct
      661 ctcccggacg agtccgggct cgcccccaac ctgtctggac tcgtcatttc tatcgcgtca
      721 gtttatgcaa ggtttctaaa cagccgagtt ccttcccacc aaacgtgatt cgcccaaacg
      781 agacgcgcta cagatcaagg ttgtatgcat cggtcaaatc ggcaagttct gaagcacaca
      841 gtcattcatg ctgtatgcga tcgtgaccaa caattttccc gtgaggctcc gataattcag
      901 cgcggctgca cgctcttcag gagcggcggg agtagctggg accacggaca tcgcaggacc
      961 gtcatcataa ctgtagccgg acagaaaact gaaagcggcc atgcgttcac gtactttaac
     1021 aaccgactct cggtcccatt caatatcaaa acggctcttt ttgggagact tgaagacgat
     1081 actcgcgcag tcatttaaat aacccgcagc agctctgacg ccgaggaacg agcagagctg
     1141 tttaagatag ggctgaggat cgtcgataaa cgattccagc cgaacatcga aaatttcgtt
     1201 gggatccatt cgcgttttta ggctctcaat gctcgaacag agacggaaaa acacatcgat
     1261 gtgcatcgcc aacgttttgt gcgggctgcg ccgcgccatc gtagcgagga cgtcataggg
     1321 attgcgaata acgtgaacga actttagctc gccgccgatt tttgttctga gagacgcgag
     1381 caaccgaggt tcacggtaga agcgcatagc cgacgtcgcg ccctttttat cgcccaaaac
     1441 ctgcaactgc tcgaaccggc cttgccactg attcggcacg tcgtaggaat aaccactgtg
     1501 gctgcgcccc gatacagcca tcttccggga attttccagg agcaagttga acacctggcg
     1561 ccgagtaaac ccgtgcttta ctaaccttaa cgcgtccaac tcgtgcgcga tgacaatgtt
     1621 cggatgggca tcgagtagag atccgatcag actgtgtcct gttcttggat agccgacaaa
     1681 catgcaataa gtttgaaagt cgcgaaaaaa cgcgctgttt tttctaattc gcactaaaga
     1741 gcgggcgtac aagccgagca gatcgagttt ttttccaaac gacataaggt cacgcgtgtt
     1801 gtctattgat tctcgccgcg attattcgaa ataagctccc tcgatgagaa ccggttttgc
     1861 cgcgccttgc tcatgggtcc ttcccgggat agccgaacca tcggggttga gccgctggtc
     1921 tgccttgtct tggccggcgc cgatgcgcgc catcaccaac ggggtctggc ccgaattgca
     1981 aaactgatag tagctgcccg ccggcagaac caccgcgtga ttcttggttg cggtcagcac
     2041 cgtctggcca tctcgttcag tgttgaactg cgctgttccc tctagcacca cgaaaatgtg
     2101 gtcttctttg gtatgcgcgt gaagcgcgtt ttccccaatt ccaggggcat aacagtgaat
     2161 tctgagcgtc atcaaatcag tttccgccag catgaaccgg gtatggcctt ccctcactag
     2221 ctgggtcttg agttgcacca cttgcgcttt tggatccata aaacctccct tgcgatttac
     2281 tatgaaagcg cggcgatctt agaatagacc gggctcctag cgcaagacgg cctgcgcagc
     2341 gatcgtcaat tccgctcgat tctcgttcac tcgatcgaca gcggttgcaa atctcagctg
     2401 cctcggttta gctaaaattt tcacggataa cacccacctt gacgccgcta tttgctgaaa
     2461 tcgtcgcaat cttttccgcc atgggctggg ccatcgattc ggtgttggtt cgcttcggcc
     2521 tgcgccaatc caacatcttc gccgccatgc tgatgagcta cggcgtttcg atcacctgcg
     2581 tttggagtta tctgatcgcc accacttcgc tggaatttct tagctcgcca gtgatgcttt
     2641 actacgtaat cagcggctgc atgcagccga tttttgcgcg cgcgctgttt tatcagggca
     2701 tcaccagaat tggtgtcgca cgagccgggc cgctgcgcgg catcgagccg cttttcgccg
     2761 cggccatcgc tttgctggtg ttcaatgaac agcccggact gcaagtctac atcggcaccg
     2821 tactcatcgt cacgagcctc tggctgattt ccggcaagca ggaacaaaac aatccgtggc
     2881 gctacatcga cgcgctgtta ccgatcagcg ccgccttgat ctcggcgatc tcgcagactc
     2941 tgcgcaagca agcgctgcag atcattcccg atccgtttat cgccgttgcg atggtcacca
     3001 gcgtctcgct gtttctgttg ttgggcttcg tctacgccag cggccgcgca cagcaattgc
     3061 gtatgaaccg cagcagcttt aagttttttc tcggcgctgc attggttgcc accaccgcgc
     3121 aaatcttcaa tttcatcgcc ttgggccgcg gccaaatgtc ggtcatcatt cccctgctca
     3181 ataccacgcc gctgttcacg gtttttttta gcgctctctt tctgcgcaaa atcgaaacgg
     3241 tcaacctgcg catcatcctc ggtgctgccc tgatggtcgc cggcgtcgtg tgcatcacca
     3301 gccggtagcc cctggcgtac ttgactgaat aactttttcc gccaagatag cctcggtctc
     3361 tggagcaggt atggaaatcg acaacgaacc tcccgttgag cgtatcgacg aacaggccgc
     3421 cctgcggatg attttagaag gaaccgccag cgaaaccggc gagcggtttt ttgctgccct
     3481 ggtgcgcaat ctctccaaag cactcaacac taccggggcc tgggtgactg agtttctcga
     3541 agccgagcgc aaactgcgag ctttagcttt ctggttcgac ggtgcctgga ttccggacta
     3601 cgtcacggat cttcgtggca cgccctgtga gcaggtaatc gtcagcgccg aactggtgca
     3661 ttttccggag aatctcatcg aattatttcc agaggaccag gacgtgaaaa ccttgggcgc
     3721 ggtgagttat ttgggcatgc cgctgaaaga catcgacggc agaatcctcg gtcacctggc
     3781 ggttatcgat cgccggttcc ttcccaacga cccccgaatc ctagcgctgt ttcgtatctt
     3841 cgccgatcgc gctagcgccg agttgcgccg actacgggcg gagaaacaag ttgacgagcg
     3901 tgaagcaaaa ctccgtcggc tggtcggcag tgccatggat gccatcgtcg agtttgacaa
     3961 tcaattgagc gtcacgctgc tcaatcccgc agccgaacaa gtctttcaac gtcaaagcgc
     4021 tgcgatcggc ggcgagaaga tcagcgcttt tttttcccaa gagagcggcg acaagctgtt
     4081 gagcctggtt cgcgccctcg aatgtcagcc caaggaccgg caatcgatgt ggattgccgg
     4141 tggcctgaca ggctgtcgca aagatcgtag cgagttcccc gccgaagctt cgttatcgcg
     4201 ctctgaatta ggcgagaata tcttttacac gctgatcctg cgaaatctca ccaatcgtct
     4261 agaggcggag cgtaaaattc tcgttttaag cgccgaagcc gagcagctcc gggcggaaat
     4321 acaatcgctc caaggctctg gcgatatcat cggcggcagc gcggcattaa agaacgtgct
     4381 gcgcgacatc gaacaggttg ctgaaacaga cgcgacggtc ctgttgctcg gcgaaaccgg
     4441 tactggcaag gaacttgtcg ccagagcgat tcacaaggcc agccggcgcg ctgacaaggc
     4501 gctcatcacc atcaactgtg ccgccatccc aacgaccttg atggaaagcg agttctttgg
     4561 tcacgagcgg ggtgccttta ccggggcgac ccagaagcgc gagggccgtt ttgccttggc
     4621 caatggcggt acgatctttt tggacgagat cgctgagctg aatcttgaac tccaggccaa
     4681 gctgctgcgc atactgcagg agggcgagtt cgagccggtt ggcagctcgc agacgcgtaa
     4741 agtgaatgtc cgggtgatcg ctgcaaccaa tcgcgatctc ggccaggcgg tgcgcaccgg
     4801 caatttccgc gatgatcttt attatcgtct caatgtcttt ccgatttcgc ttccagcgct
     4861 gcgcgaaagg ggtgacgatg tcatcgagtt ggcgaatctt ttcgccaaca aattcgccca
     4921 acgttccggc cggcgcgtcg aacccttgac cgatacaggg aagcagcggc tcaagaagta
     4981 ttcgtggccg ggcaatgtgc gcgagctgca gaatgtcatc gagcgtgccg tcatcacgtc
     5041 acaggacggg cggctgaacc tcgatcgtgc gctgcccggc gtcacggaag accagccgac
     5101 catcggtgaa aaccggtctt ctgaatcgca atccgctgaa cgcatattgc aggtaagaga
     5161 gattcagcaa ctcgaacggc agaacattct gcgcgccatg gaaaaagccg gctggcgagt
     5221 cgccggcagc gacggtgccg ccgaactcct cggcatcaat ccctccacgc taaactcacg
     5281 catcaaagct ttgggtattc agcgcctgcg ctaaagccaa ccagaacacc cgccctttgg
     5341 gcgagctggt cgctgtgtct ttgaattgct gcggttgaga tctgccggcc ctgcggccgt
     5401 gtcacagcac ccctcgtgag atctcgcgtc ggcgctaatg atatttcgtg actaacgaca
     5461 tctcgcgtca acgcgacgtt gccgctagcc ccgcaagcat ccgtatttca aacgtttgca
     5521 gtggccgaaa gctttggcat acggtctgcg ttacgcaatc gacaagcaat caatatctca
     5581 ggagggatgc gatggaacac aattactcgt atcaatcggc actggcgact tcagaacgca
     5641 tcggctggag aatcgatgat atcatcggcc ccgataagcc gttgaacttt gcaaaaccgt
     5701 tcatgcccga ggcgctggcc caagtccggc gactggactt tctcagcgct ggcgagcaac
     5761 gcacgctgaa tcagatccgt gggcacgaat acttggcgat gttcggcctg gtcgaggaat
     5821 ttattttgcc gtatgtcgtc gagcacaccc gcacaaaatt atcgggtgac gactatcgcg
     5881 tgcgcgcctt gctacaattt gccggcgagg aagccaagca tattcatcta tttaagcgtt
     5941 tccggcagca gttcgacgct gggtttggga ccccatgcga gttcatcggt cccgccgaag
     6001 agatcaagcg tttcgtcttg tcgcatagcc cgctcggcgc cggcatcgcc attctgcaca
     6061 tcgagtggat cactctgcgc cattacatcg aaggcgtgcg cgatgatcag gacctcgacc
     6121 cgcagttcaa aagtctgctg aagaatcact ggttggaaga atcacagcac actaagctcg
     6181 acaccctgat gatcgaagaa atcgccgcgg ctgcgagcca aagcgagata gaactggcca
     6241 tcgccgagta cgccgacatc ggtaaattcc tcgacaacgg cctgaagcag caggccgaat
     6301 tcaacgtcga cagttttgtc agggcgacgc gccgaaatct aaccaaatca cagcgcgaac
     6361 aagccaccga caatgtgctc aaagccatgc gctggacgta tctcggcacc ggcatgactc
     6421 atcccaattt cctcgccact gtcgagaaga tcaatccggc agcaagaaag cagatcgaag
     6481 aaatggcccc ggcattctgc tgaggcgaaa acaccgaccc taatttagat cctcggaggt
     6541 ttaccatgtc agcatcagcg gcaacaaaaa tcgctaccgt aaacggactc gatgtcgacg
     6601 ccctgcgtca ggtcgtcgac gaggtcaaaa aagatccagc aaaaggcatc gtcgagttca
     6661 aggtcaaaac cggctggaaa gggcggacgc gctcggaaac cacggtggaa ggctacaagc
     6721 tcggcggtga gaagatcaaa cgccgattca ccatcagcgc cgacgagccg ttcgaattgc
     6781 tcggcgaaaa cacttcagcc aacccgcagg aattgctgat ggccgcgctc aacgcctgca
     6841 tcacagtggg ctatgtggct ggagccgcgg tcaacggcat cactttgacg aaattggaaa
     6901 tcgaaacctc cggacaattg gacctgcgtg gctttctcgg cctcgatgcc aatgtcaaac
     6961 ccggctacga atcgctgcag tctgttgttt acattgccgg caacggcaca gcggagcagt
     7021 ttcagcagat ccacgacaac gtcatgaaaa cttcgccgaa cttcttcaac atctcacgcc
     7081 ccgtgcgaat tgacgcgacg ttgaaggttg aaaattgaca ctcgctcaat ccagcgggaa
     7141 cgatcaagat cacagcacaa acgaaatgta gcggtcggag ttattgcggc aagcactaaa
     7201 gtatgtg
//
LOCUS       DATLCH010000116         4202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2600805, whole genome shotgun sequence.
ACCESSION   DATLCH010000116 DATLCH010000000
VERSION     DATLCH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2600805"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1069)
                     /locus_tag="VMZ02_03410"
     CDS             complement(50..1069)
                     /locus_tag="VMZ02_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71008.1"
                     /translation="MLAGYFFMLLVFGGFLSAPLSAQESFYRGKTLRLVVGFSAGGGF
                     DVYSRAISRHIGRHIPGSPAVVVENMTGAGSMVAANHLFRAAKTDGLTIGNVNGGLFV
                     QQLLGWPGIEFDAAKFEYLGVPVKDKAVCAMTKASGIASYERWAASKIPVKLGATGPG
                     SATHNVPLILKEALGLPIQLVSGYKGIADVRLAAEGGELSGVCGWTWDSIKATWAGAL
                     DSGDAIVVLQTVPQAISELPKVPLAISMAKSDAAKQLIQAGIHDVADMTYSYVLPPGM
                     AKDRADTLRKAFGDTMKDPEFLADTKKSRLGIDPMTGEELQRTIAGLFKTPAGVVAKL
                     KEVLK"
     gene            1231..2214
                     /locus_tag="VMZ02_03415"
     CDS             1231..2214
                     /locus_tag="VMZ02_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71009.1"
                     /translation="MMNSIKLRIFAMTAAAFLLTPIGSQSQDKPAPEKVRAAIATSSL
                     AFLVSFVAKDRGFYLKYGSDVELIVMRPNIAMAALLGGDIDYAELIGSVIRSAARNLP
                     VRAISTGIKAPFFSVIGQNKFKSVKDLKGAIIGLTSIAGTNHISVRMTLKQFGLDIDK
                     DVKILAIGDERLVYDTFKLGRVDAVALAPPFSVQLKREGYPVLAQTSDYVVIPFSGLG
                     ATLEKIKTNRAQVKRMLKAEIEALRFIHANAAGTTEVIRKRFNMDEKLARESYDAVVN
                     SFSRDGRVPLDGVDILLQIEKDAKAIPATVTPQMIVEASLVEEALKELGGK"
     gene            complement(2345..3346)
                     /locus_tag="VMZ02_03420"
     CDS             complement(2345..3346)
                     /locus_tag="VMZ02_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71010.1"
                     /translation="MKSTSVRPKLSNIVFGFLLAVIVPVSVGAETAVPIGSTVTFSVA
                     ENGQAGCRRAEIGVPCGNFLGETAGRPSTREVETSARIGTLQAAGFIGVPIYSTASVY
                     NDFFIPGPPDGNMVDVQITVQYDFTGNILGGGMYAMSNSLALRVQDRTANALVARQEF
                     VSMERQGDQGIELGVAAAQERSVLTGEIGHMLVKLRRGHHYRLHFELESSASTFLAGA
                     MRADAIAKWNRLSVNVGEDEVELLDIHDQEVKAALLGVQSAILRALAKHDIDIKAELG
                     IIKNQLSGIDGDLEEIKTLLLTPQGRREGFPILPNNPNPNSTTTPAPTATSKSKPKK"
BASE COUNT          970 a         1208 c         1097 g          927 t
ORIGIN      
        1 acaatgagcg ccgggccgcc ggagttctga tcgaccatgg ttgcggcgct catttcaaga
       61 cttctttcag cttcgccacc accccagcgg gggttttgaa cagaccggcg atggtgcgtt
      121 gcaattcctc tccagtcatc ggatcgatcc ccaaacggga tttcttggtg tcggcaagga
      181 actcaggatc tttcatcgtg tcgccaaacg ctttccgcaa agtatcggcg cggtccttcg
      241 ccatgccggg cggcaaaacg tatgaatagg tcatgtcggc gacatcgtga attcccgcct
      301 ggatcagctg cttggcggcg tcgctcttgg ccatgctaat cgccaaagga acctttggca
      361 gctcgctgat cgcttgcggc acagtctgca gcacgacaat cgcgtccccg ctgtccaacg
      421 cccccgccca agtagccttg atcgagtccc aagtccaacc acaaaccccg gacaattcgc
      481 cgccctcggc ggccagccgg acatcggcga tgcctttgta tcccgagacc aattggatcg
      541 gcaggcccaa cgcttctttc aggatcagag gcacgttatg cgttgccgag cctggtccag
      601 tggcgccgag tttgaccgga atctttgacg cggcccatct ttcgtaactg gcaatgccgc
      661 tggccttggt cattgcgcac acggctttgt ctttgaccgg cacgcccaga tattcgaact
      721 tcgccgcatc aaattcgatg cccggccagc cgagaagttg ctggacgaac aacccgccgt
      781 tgacgttgcc gatggttaga ccgtccgttt ttgccgcgcg aaatagatga ttggccgcaa
      841 ccatgctgcc ggctccggtc atattctcca ccaccaccgc cgggctgcca gggatatgcc
      901 gtccgatgtg gcgcgagatc gcccggctgt agacatcgaa accgccgccg gcgctaaaac
      961 cgaccaccag ccgcaaggtt ttgccgcgat agaagctctc ctgggccgat aacggcgccg
     1021 agagaaagcc gccgaacaca agcaacatga agaaatagcc agccaacatc tacactctcc
     1081 ggtggcaacg caaataaata acgattgccg cgaaactatg cgaagcaaat aaagctgtca
     1141 accaaggccg gaggagaagt tgagttgagc gaatctttcg gccatattca tgcggattgg
     1201 cgttgcgccg agaaatggag gggaatttcg atgatgaatt ccatcaagct aagaatattt
     1261 gcgatgaccg ccgcggcatt cctgctcacc cctatcggtt cgcaatccca agacaaaccc
     1321 gccccggaga aagtccgcgc cgccatcgct accagctcat tggcctttct tgtgtcgttc
     1381 gtcgccaagg accgcggctt ttatctcaaa tatggctcgg acgtggagct gatcgtcatg
     1441 cgtcccaaca tcgcgatggc cgcgctgctc ggcggcgaca ttgactacgc cgagctaatc
     1501 ggctcggtga tccgctcggc ggcgcgcaat ttgccggtgc gagcgatttc gaccggcatc
     1561 aaagcgccgt tcttcagcgt catcggccag aataagttca agagcgttaa agacctaaaa
     1621 ggcgcgatca tcggcctcac ctccatcgcc ggcaccaatc acattagcgt gcgcatgact
     1681 ctaaaacagt tcggcctcga catcgacaag gatgttaaga tcctcgctat tggcgacgaa
     1741 agattagtat acgacacgtt taaactcggg cgggttgatg cggtcgcgct cgcgccgccg
     1801 ttttccgtgc agctcaaacg cgagggctat ccggtcctcg cccagacgtc cgactacgtc
     1861 gtcatcccat tctccggcct cggcgcaacc ctggagaaga taaaaacgaa ccgcgcccag
     1921 gtcaaacgga tgctcaaagc ggagatcgaa gcgctgcgct tcattcatgc caatgcggct
     1981 ggcacaactg aagtaatacg caagcgcttc aacatggacg aaaaactggc gcgcgaatcc
     2041 tatgacgccg tggtgaactc cttcagccgt gacggccgcg tccctctcga cggcgtcgac
     2101 atcctgttgc agatcgaaaa agacgccaag gccatccccg cgacggtcac gccgcaaatg
     2161 atcgtcgaag cgtcgttagt ggaagaagcg ttgaaggagt tgggtgggaa gtgatccgcg
     2221 caagcgatca aaaggaattg ccctcaggcg aggcaaggac gcaccggttt acgtttacgc
     2281 ccttgcctcg atttactctt gatgattgct tcaagacttt acgtcgcgcc agcagtcagc
     2341 ctgcttactt cttcggtttc gatttcgaag ttgccgtagg tgcaggtgtt gtggtcgagt
     2401 ttggattagg gttgttcggc aaaatcggaa atccctcccg cctaccttga ggtgtcagca
     2461 gcaaggtctt gatctcttcc aagtctccgt caatgccgct gagctgattc ttaattatcc
     2521 ccagctccgc cttgatgtcg atgtcatgct tggcaagcgc gcgcaatatg gccgattgca
     2581 ccccgagtag cgcggctttt acctcttgat cgtgtatgtc taaaagctcc acttcgtcct
     2641 ctcccacgtt aaccgacaat ctgttccact tggcgatcgc atcggcgcgc atagcccctg
     2701 cgagaaaagt agaagcgctc gattccaatt cgaaatggag ccggtaatga tgaccgcgac
     2761 ggagcttcac cagcatatgt cctatctcac ccgtaaggac actgcgttct tgcgccgcgg
     2821 ctactcctaa ttcaattccc tgatccccct ggcgttccat actgacgaac tcttggcgcg
     2881 ccacaagtgc attcgcggtg cggtcctgta ctctgagcgc gagcgaattg ctcatcgcat
     2941 acataccgcc tccaagaata tttccggtga aatcgtactg taccgtgatc tgaacatcga
     3001 ccatgttgcc gtccggtggg ccgggaataa aaaaatcgtt gtatactgaa gccgtcgaat
     3061 agataggtac cccgataaaa ccagccgcct gaagagttcc gattcgagcg ctagtctcga
     3121 cttcccgcgt ggatggtcta ccggccgttt cacccaaaaa gttaccgcag ggaacgccga
     3181 tttcggcccg gcgacaacct gcttgaccat tttctgccac cgaaaaagtg accgtactcc
     3241 cgatcggcac cgctgtttca gcgccgacag ataccggaac aatcactgcc agcaaaaaac
     3301 caaagactat attcgataat tttggtctca cggatgtaga cttcatagtt tctcccgatg
     3361 aattttctac gttgtattga acacaccact agtcacagaa tcgataagtt atcgtatcgc
     3421 tggccaggac ctcgatcctc gtcatccgcg ggtggtgtga cagtcccgac aagcaacccg
     3481 acttgcgacc actgctaacg aaaagttctc agggaaaaat ccatgtgatt gaagttgctt
     3541 tttgaacagc ggttattcgg cgggtaaaga cgcaccgagc agcgaaagat ttgcgtaagg
     3601 tgtcggcgca tttcgaacga gccgtccatg gcttcgaaca aaataaaaga ttggatcgaa
     3661 tcgtcgatca tattcgcagc gcaccccacc cgacacttcg gccttattcg aaaaccccga
     3721 ttttggcagt cggtaatatg ccgacttatg tggcgagtca acgaaaatcg gtaagtgcga
     3781 atgaaaatgt ttgaggtttg ggaactttat ggaggtttag agaatttacg ggcgttctag
     3841 tgggaaaact tctaaaatgg acgcgccatc gatggcgtcg acatcctgct gaatatcgca
     3901 gaatgcgcaa aaataatccc agcggccatc actccgcaat gatcgtcgaa ccgtcgttgg
     3961 tgaaagaagc cttggaggag ttgggcggac aatagttgcg aaggccggat ttagtctatt
     4021 tggggagggt tcacgctaat ttcacctgct caatggcaat cactgacctg cgtgaatagc
     4081 tcagggccct tgggcagccg ggcggttccg aactccacag tgagaactcg attcttcctt
     4141 tcgtcattcc ggcgtaggcc ggaatccaac gtttaacgat cagcgacatt cgagcctgga
     4201 tg
//
LOCUS       DATLCH010000117         6901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10619401, whole genome shotgun sequence.
ACCESSION   DATLCH010000117 DATLCH010000000
VERSION     DATLCH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10619401"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VMZ02_03425"
     CDS             complement(<1..1086)
                     /locus_tag="VMZ02_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HUR71011.1"
                     /translation="MRIFLSYASQDREMAKSIYLALRDQRHTVFFDRADLPAGDEYHN
                     RIRSAIENSDLFMFLISANAVDAGSYTLTELSIAEKALIKVLPVVLGNVDVEKLPALL
                     KAVTFFQTDGNLPAAVAAEVYRIAKERRRRRLKYLSAALVVALVVVGGVFYALRGRDS
                     RERVGKDGASAVLIPAGAFLGGDDENSPRRELFVEAFYLDRYEVTVGRYARFLQATGN
                     VRPPEEWDTVDLNNGTDLPVIGVDWQDANSYCQWVGRRLPTDAEWERAARGMDERKYP
                     WGNEPPTPEHARFAQKYEKAVYKGGVARVGSHRKGASPFGIHDMAGNAWEWTADWFSE
                     SFPYADRRNPKGPSSGTSKVLRGGGWYD"
     gene            complement(1137..2195)
                     /locus_tag="VMZ02_03430"
     CDS             complement(1137..2195)
                     /locus_tag="VMZ02_03430"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71012.1"
                     /translation="MCSMFVSLARSLVLFAVISVGSGDWARANPYLAKSGEPPVRARI
                     GTCAITGGFIHLYAALDNKLFDKYGVIAEHIVVRGGSVAAAALAADEISFLYCNADAN
                     IARIATGMDGKLVASPLVGLPYVVLARKDIKRPADLKGKTIGVTRPGDFTYKLAKDFL
                     KKHNLSDKDVTLATVGGTPTERYTALVQDVFQAILIQPPLDARGRKDGFNVIYHLSDL
                     GLPFIYSSLFVNAKTFKEKPALVQKMVAALAESVFLVEKKPEVGMASVGKTLRINDAD
                     TLQSAYDAYAKRLINRRLIVPAKMVAETMENAREEGTVIRRKPNEVFDNMFVENLDKS
                     GFMKELWGGKVPEETRKP"
     gene            complement(2330..2932)
                     /locus_tag="VMZ02_03435"
     CDS             complement(2330..2932)
                     /locus_tag="VMZ02_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HUR71013.1"
                     /translation="MQDNDLRICFIGDSFVHGSEDPEYLGWTGRVSILARERGYKLTC
                     YDLGVRRETSSEIAARWELEARRRLPHPCTPYVVFSYGVNDTTVEEGRTRVPQLVSIE
                     NTRQILQTARQCDYRTALIGPPPIADRQHNDRTRQLSLAMGQVAKAEGVEFLSTFDAL
                     YADAVWMAEVSAGDGAHPGAAGYARFAALVDAWKGWWFRK"
     gene            3220..3708
                     /locus_tag="VMZ02_03440"
     CDS             3220..3708
                     /locus_tag="VMZ02_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71014.1"
                     /translation="MPKITSISHIVLYVKDLDKMVDFYTNMLGLVKYRENAGRMVFLT
                     PDPELDDHQLALVKGREGDAKLIAHIAWKVETPAEIKEFYDRFKAAGVPIDHCVSHAY
                     AEMGNTVSCYFLDPEGNRIEVYALVAERDDARENRPLDLDKSLGEIVAVASGRAPASA
                     AH"
     gene            complement(3723..4445)
                     /locus_tag="VMZ02_03445"
     CDS             complement(3723..4445)
                     /locus_tag="VMZ02_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR71015.1"
                     /translation="MDAVNTEFRVMTSGAFTAAYLELIPQLEALIKKKIVTAATSIGT
                     GENSIPNRLKRGEAVDVIIVADGVLSDFIKDGLVLADGYTPLVRSAIGMAVRAGAAKP
                     DITSVDALKRTLLQAKSIAYSASVSGDYLTKELYQRLGIADQVMSKSRLIGGGERVGA
                     VIARGEAEIGFQQMSELMPVPGIAHVTPLPPEVQKVSVFSAGVGKTTTAIAAAHAVIK
                     FLASPAAASAIIKSGLEPVGTR"
     gene            complement(4581..5063)
                     /locus_tag="VMZ02_03450"
     CDS             complement(4581..5063)
                     /locus_tag="VMZ02_03450"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR71016.1"
                     /translation="MANTQYPIAIIAADAALRTKPTNYPEPFASRMAGREKRPLGDPF
                     GLTNFGVNLTRLVPGGSSALRHAHSKQDEFVYILTGRAVLITDDGETPLLPGMCAGFK
                     AGTGNGHHLVNRGNEDVVYLEVGDRSAGDGGSYPDDDLQAVLDGDGKWQFLHKDGAPY
                     "
     gene            complement(5084..5455)
                     /locus_tag="VMZ02_03455"
     CDS             complement(5084..5455)
                     /locus_tag="VMZ02_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71017.1"
                     /translation="MANNPVGWFEIYVQDMARAKKFYEAVFQLKLERLNNPELEMWSF
                     PMAMDRAGASGALVKMDGFPSGGNSTLVYFTCADCAVEAGRVTASGGRIQREKISIGQ
                     YGFIALAFDTEGNMFGLHSMQ"
     gene            complement(5557..5826)
                     /locus_tag="VMZ02_03460"
     CDS             complement(5557..5826)
                     /locus_tag="VMZ02_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71018.1"
                     /translation="MVAKFRSVKPQIKYHRDGSLWAKGQILDGVLTGYWEWFRKDGTK
                     MRSGTFDKGQQVGEWTTYDKHGRVYKVTNVKPATPKKPRFRPAPE"
     gene            complement(5872..6261)
                     /locus_tag="VMZ02_03465"
     CDS             complement(5872..6261)
                     /locus_tag="VMZ02_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71019.1"
                     /translation="MTIHLDHTIVPSRKAVDSAKLLAELLGVPWAATSAGPFSPVYVN
                     DGLTLDFIETDEDFPIYHFCFRVEQEEFDSILERIKAAGIKYRSTVRGPVDMQINTEL
                     GGSNIYWNEPDGHQWEMLTVPYARPAA"
     gene            complement(6322..6786)
                     /locus_tag="VMZ02_03470"
     CDS             complement(6322..6786)
                     /locus_tag="VMZ02_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUR71020.1"
                     /translation="MATIYHQVWINAPVARVYELISSAEGISRWWDKQTATRTDDGLI
                     FEHNPGPEHGVVKLKVLEMVPNKRVEWECISIHNKTTPAFAWTGTHLTFELTEREKFA
                     PANVSWAAGVPALAILDFRHSGWAESSDYFGFCNYAWATVLQQLAQLCESEE"
BASE COUNT         1496 a         2022 c         1797 g         1586 t
ORIGIN      
        1 gtcgtaccag ccgccgccgc gcagcacttt actggtgccg ctgctgggtc ccttgggatt
       61 gcgccggtct gcgtacggga agctctccga aaaccaatct gccgtccatt cccaggcgtt
      121 accggccatg tcgtgaatgc cgaaaggact tgcgccttta cggtggctgc cgactcgagc
      181 gacgccgcct ttataaactg ctttctcgta cttctgagca aagcgcgcat gctcgggcgt
      241 agggggctcg ttgccccacg ggtatttacg ttcgtccatg ccgcgtgctg cccgctccca
      301 ctcggcatcg gtcgggaggc gccggcctac ccactggcaa taactatttg catcttgcca
      361 gtccacgccg atcaccggca aatcagtgcc attgtttaaa tcaaccgtgt cccattcctc
      421 gggcggtctg acgtttccag tggcctgcaa gaaacgagcg taacgtccga cggttacttc
      481 ataccgatcg aggtaaaatg cctcgacgaa gagctcgcgc cgaggcgagt tttcatcatc
      541 gcctccgaga aaggccccgg ccgggatcaa aacagcagac gcgccatctt tgccgactcg
      601 ttccctgcta tccctgccgc gcagagcata aaacactccg ccgacgacca ctagggcgac
      661 caccagcgct gcgctcaggt acttgagccg tcgtcgccgg cgctccttgg caatgcgata
      721 aacctcggca gcaactgcag cgggcaggtt gccgtcagtt tgaaaaaacg tcaccgcttt
      781 gagtagagcc ggtaattttt caacgtcgac gtttccaagc accaccggca acaccttgat
      841 cagcgctttt tcggcaatgc ttagctcggt caaggtgtaa ctgccggcat cgacagcatt
      901 ggcgctgatc aaaaacataa acagatcgga gttctcgatc gccgaccgaa tgcggttatg
      961 atactcgtcc ccggccggca gatcggcgcg gtcgaagaac actgtatggc gctgatcgcg
     1021 tagtgccaga tagatggatt tcgccatttc gcgatcttgc gaagcgtaac taagaaaaat
     1081 tctcatcgag tcgcttaccg ggcgccatga ctctcagtca aggatgcata gtacttctac
     1141 ggctttctcg tttcttcggg aactttgccg ccccatagct ctttcatgaa gccgctcttg
     1201 tcgagattct cgacgaacat gttgtcaaac acctcgttcg gtttgcggcg aattactgtg
     1261 ccctcttcgc gggcattttc catggtctcg gcaaccattt tcgccggcac gatcaggcgc
     1321 cgattaatca agcgcttggc gtaggcatcg taagccgatt gcagggtatc ggcatcgttg
     1381 atccgcaacg tctttcccac cgacgccatg ccaacctccg gcttcttctc gacgagaaat
     1441 accgactcgg ccagtgccgc gaccatcttt tggaccaacg ccggcttctc tttgaaagtc
     1501 ttggcgttaa cgaacagcga actatagatg aacgggagcc cgaggtcgct gagatgatag
     1561 atcacgttga agccgtcttt cctgccgcgg gcatcgagcg ggggctggat caggatggct
     1621 tgaaatacat cttgcaccag cgcggtgtaa cgttccgtcg gcgtaccgcc gactgtcgcc
     1681 agagtaacat ccttatcgct caggttatgc ttcttgagga aatccttcgc caatttgtaa
     1741 gtaaaatcgc ccggccgcgt cacgccaatg gtcttgcctt tgagatcggc gggacgcttg
     1801 atgtccttgc gcgccaagac gacatagggc agccccacga gcggagaagc gaccagcttg
     1861 ccgtccatac cggtggcaat gcgcgcgatg ttcgcgtcgg cgttgcaata aaggaaactg
     1921 atttcatccg cagccaaagc cgccgccgcg acactaccgc cgcgcacgac gatatgctcg
     1981 gcgatgacgc catacttgtc gaatagtttg ttatcgagtg cggcgtagag atgaatgaac
     2041 ccgccggtaa tcgcgcaggt gccgatgcgc gcacgaacgg gcggctcgcc ggacttggca
     2101 agatatggat tggctcgtgc ccagtctccc gagcctacgc taataactgc aaataaaact
     2161 aaacttctgg ccaagctcac aaacatcgag cacatgcagc accccgttca ttatttttta
     2221 atctgcccta tatacgagct ttcaatcgag agggtcaatg gactaacgac agttttgaga
     2281 atgccaaatg cagatgcggc ttcgcgctcg tcacggggcc ggcgctgcat cactttctga
     2341 accaccaacc tttccacgcg tcgaccaacg cggcgaatct cgcatagccc gctgcaccgg
     2401 gatgggcgcc atcgccggca gatacttcag ccatccatac tgcgtctgca tacaaagcat
     2461 cgaaggtcga cagaaactca actccttcag ctttggcaac ttgccccatg gccagcgaca
     2521 attgacgagt gcgatcatta tgctgtcgat cggcaatcgg cggagggccg atcagcgcag
     2581 tgcggtaatc gcattgccgc gctgtttgca gaatctgtcg agtattctcg atactgacta
     2641 actggggaac tcgcgtgcga ccctcctcga ccgtggtgtc gttgacgcca taggaaaaca
     2701 ccacatacgg tgtacacgga tgcggcaagc gccgccgagc ttccaactcc cagcgtgcgg
     2761 caatctcgct gctggtctca cgccgcactc ccaaatcgta gcaagtcagc ttgtaacctc
     2821 gctcccgggc cagaatcgat actcggccgg tccaaccgag atactccgga tcctcactac
     2881 catggacgaa agaatcgccg atgaagcaaa ttcgcaaatc gttatcttgc atggctcccc
     2941 atcgtctttt attttatggc gttaagcggg cgattattgg tcggaatgga agactcgagg
     3001 gtgcggttgc gtttaatgga cgtggtgtcg ccaccatcac cgacatcttt atgcatttaa
     3061 ccgacgaatc acacgtaact cctcagggcg cgttcattcc ggttgtccgc cggcggtcac
     3121 tcgcgaaagt ttaatttgac atcggacgat cgggttggat agtgtcgggt tcagccgaat
     3181 gacccgtcac aacagccagc cgttccgaag gaggtttata tgccgaagat taccagcatc
     3241 agtcatatcg tgctttacgt taaggatctg gacaagatgg tcgacttcta caccaacatg
     3301 ctggggttag ttaagtaccg tgaaaatgcc ggtcggatgg tttttctcac tcccgatcca
     3361 gagttagacg atcaccaact cgctctcgtc aaaggccgtg aaggcgacgc caagctcatc
     3421 gcccatatcg cctggaaggt ggagacaccg gcggagatca aagaattcta cgaccgtttc
     3481 aaagccgcgg gcgtgcccat cgaccattgc gtcagccatg cttacgccga gatgggcaac
     3541 accgtctcat gctatttcct cgatcccgaa ggcaaccgca tcgaagtcta tgccctggtc
     3601 gccgagcgcg acgacgcgcg cgaaaaccgc ccattggatt tggacaagag cttaggtgaa
     3661 atcgtcgccg tcgcgagcgg tcgggcgccc gcgagtgccg cgcactgaac cactattacg
     3721 cttcaacgag ttccgaccgg ctccagtcca cttttgataa tggcgctggc cgccgcgggc
     3781 gaggcaagaa atttgatcac cgcgtgagcc gcggcgatag ccgtagtagt tttaccgacc
     3841 cccgccgaga ataccgaaac cttctgcact tccggcggca gtggtgtgac atgcgcaata
     3901 ccaggtacgg gcatgagctc gctcatctgc tggaagccaa tctccgcttc accgcgcgca
     3961 atcactgcgc cgacgcgctc gccgccgcca atgagcctgc tcttgctcat cacctggtcg
     4021 gcgattccca accgctggta aagttctttg gtgagataat cgccgctgac gctagccgag
     4081 taggcaatcg atttggcttg gaggagcgtg cgcttcaacg cgtccaccga agtgatatcg
     4141 ggcttggctg ctcccgcccg caccgccata ccgatggccg aacggaccag tggagtatag
     4201 ccatcggcca gaactaaccc gtcttttata aaatcgctaa gtacgccgtc cgcgacgatg
     4261 atcacgtcga cggcctcgcc gcgttttagc cgattgggaa ttgaattctc cccagttcca
     4321 atggaggtcg cggcggtaac gatctttttc ttgatcaagg cttcgagttg aggaatcagc
     4381 tcaaggtacg ccgcggtaaa agcgcctgag gtcatcactc tgaattcggt gtttacagca
     4441 tccatgttcg atttctttca gcgctcgtcg ttattggctc gagccgttgt tgcaaatctt
     4501 tttcccagag gatttatccg tcgaagaaaa aagccagtta agtcatctca tactcttcct
     4561 tggccggtcg acgccgcgtt tcaatatggc gcaccatcct tatgcaggaa ctgccattta
     4621 ccgtcaccgt ccaacactgc ttgtaaatcg tcgtctggat agctcccgcc atccccggcg
     4681 ctacggtcgc cgacctcgag atagacaacg tcttcgttgc ctcgattaac cagatgatgg
     4741 ccatttccgg taccggcttt aaaaccggcg cacattcctg gcaaaagcgg ggtctcaccg
     4801 tcatcggtga tgagtaccgc gcgtcccgtc aagatgtaaa cgaactcatc ttgctttgag
     4861 tgggcgtggc gcagcgcaga cgagccgccc ggaacaaggc gggttagatt cacaccgaaa
     4921 ttggtcaggc cgaacggatc accgagcggc cgcttctctc ggcccgccat gcgcgatgca
     4981 aatggctcgg gataatttgt cggcttggtt cgcagtgccg catccgcggc aatgatcgct
     5041 atggggtatt gagtatttgc cataagttct ggcctcgtgg tcactattgc attgaatgca
     5101 aaccaaacat attgccttcg gtatcaaagg ccaaggcaat gaatccgtac tggccaatgg
     5161 agattttttc tcgttgtatg cggccgccgg atgccgtaac gcgccccgct tccacggcgc
     5221 aatcggcgca agtgaaataa accagcgtac tgttgccacc cgaggggaaa ccatccatct
     5281 tgaccagtgc acccgaggcc ccggctcggt ccatcgccat gggaaaactc cacatctcca
     5341 attctgggtt gtttagcctc tccaacttaa gctgaaaaac cgcttcataa aacttcttcg
     5401 cccgagccat gtcttgcacg tatatttcaa accagccgac cggattgttt gccatttatg
     5461 actcctttca gttggattaa cggtggtttc gaatactagc tgcctgaact aaacggccct
     5521 cggcctaagc cgaatggaaa tcgacttttc tttccgtcat tccggcgcag gccggaatct
     5581 aggcttcttc ggcgttgcag gcttgacgtt cgtcactttg taaacccgac cgtgcttatc
     5641 gtaggtcgtc cactcaccaa cctgctggcc tttgtcaaag gtgccggagc gcatcttcgt
     5701 tccgtccttg cgaaaccatt cccagtaccc tgttagcacg ccgtcgagaa tttgcccttt
     5761 ggcccacaag cttccgtcgc gatggtattt gatctgcggt ttgacggagc ggaatttcgc
     5821 gaccatagct agtcacttca ggcaaatcat agtctcacag agttgcttcg gtcacgctgc
     5881 cggccgggca tagggtactg ttagcatctc ccactggtga ccatccggtt cgttccagta
     5941 gatgttgctg ccaccgagct ccgtgttgat ctgcatatcg acgggaccgc gcacagtgct
     6001 gcgatatttg attccagcgg ccttgatgcg ttcaagtatc gaatcgaact cttcctgctc
     6061 gacgcgaaaa caaaagtgat agatcggaaa gtcttcgtcg gtttcaataa agtcgagcgt
     6121 cagtccatca ttgacgtaga ccggcgaaaa cgggcctgcg ctagttgctg cccagggaac
     6181 ccccaaaagc tcagccaaga gttttgccga atccactgct ttgcgcgagg gcacgatggt
     6241 atgatcgaga tggatcgtca tatcagtttc ctacagtcgg tgaacagttc tatcgacgtt
     6301 ccgcctggat tgtgtatcgc gtcactcctc ggactcgcat agctgtgcaa gctgttgcag
     6361 cacagttgcc caggcgtaat tgcagaagcc gaaatagtca ctggactcag cccagccgga
     6421 atggcgaaaa tcgagaatgg ccagcgccgg cactccggct gcccacgaga cgttggccgg
     6481 cgcaaacttc tctcgttcgg tgagttcaaa agttaaatgc gtgccggtcc aggcaaacgc
     6541 aggagtagtc ttattgtgaa tactaatgca ttcccactcg acgcgtttat tgggaaccat
     6601 ctcaagcact ttcagtttca cgacaccgtg ctctgggcct ggattgtgct cgaatattag
     6661 accgtcatct gttcttgtcg ctgtttgctt atcccaccag cgactgattc cttccgcgct
     6721 ggaaatcaac tcgtagaccc tggccactgg agcattgatc catacttgat ggtatatcgt
     6781 tgccatcgtg attctccttt gagaagccca agcctcgaga tctccgggta acgatttaca
     6841 acgtcgacat aattagagtt cagcgcactt cggcaatcct cgcgatgatc acgtcgccgt
     6901 c
//
LOCUS       DATLCH010000118         4629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9308544, whole genome shotgun sequence.
ACCESSION   DATLCH010000118 DATLCH010000000
VERSION     DATLCH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9308544"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..2695
                     /gene="gyrB"
                     /locus_tag="VMZ02_03475"
     CDS             254..2695
                     /gene="gyrB"
                     /locus_tag="VMZ02_03475"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HUR71021.1"
                     /translation="MADEKNPPVAEYGADNIKVLEGLEAVRKRPGMYIGDTSERGLHH
                     LVYEVVDNSIDEALAGHCNSIQVQIHIDNSVTVIDNGRGIPVDIHPTEKISAAEVVLT
                     KLHAGGKFDKNSYKVSGGLHGVGISVVNALSETLEIEIKRDGKVYQQSYRRGDPQGPL
                     KEVGKSKERGTRVTFKPDTQIFEVSEFSFDILSQRLRELAFLNRGLKITIEDERTQKN
                     HEFFYEGGLLSFVQHLNRAKSAIHSKVVHCEGEKEGVGVEIAMQWNDSYAENVFSFAN
                     NINTIEGGTHLIGFRSALTRSINNYAIGSGQLKKDDENLQGDDVREGLTAVISVKVPE
                     PQFEGQTKTKLGNSEVKGIVEALVNEKLGNYLSEHPADAKKIVAKGVEAARVREATRK
                     AKDLARRKGALDSGSLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRDRRNQAILP
                     LKGKILNVEKARFDKMLSSQEIRLMITALGTGVKEDYDQAKLRYHSIIIMTDADVDGS
                     HIRTLLLTFFYRQMPELVEKGYLFIAQPPLYKIKKGKQERYLKDEAMLEDHLIELGTE
                     NVRLRAAQNGNANGNGLAGQPLQGFVKKVARWEKLLISLGRKRKNRTVVEALLLEDGF
                     GEEMLKDREALLRLEGRLATYVGLTAPEQSPVSCALEEDAEHNCFKLVVTTRANGTGY
                     QTVIDREFLTTPEFREIKKLFGEMAGIGMPPYSIEDGDDKAHLKSLGAILSYVMEGGK
                     KGQYIQRYKGLGEMNPEQLWETTMNPDTRVLLQVRIEDAVEADQIFSTLMGDEVEPRR
                     KFIEDNALTVRNLDI"
     gene            2767..>4629
                     /gene="gyrA"
                     /locus_tag="VMZ02_03480"
     CDS             2767..>4629
                     /gene="gyrA"
                     /locus_tag="VMZ02_03480"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HUR71022.1"
                     /translation="MEATIKPQKVPVYIEEEMRESFMAYAMSVIISRALPDVRDGLKP
                     VHRRVLYAMYDASNTSDKPYKKSARLVGDIMGKYHPHGDTAIYDTVVRMAQDFNLRYP
                     LVDGQGNFGSIDGDNPAAMRYTEIRMTLLAEEMLADIEKETVDFVPNYDDSLKEPAVL
                     PSRIPNLLINGSAGIAVGMATNIPPHNLNEVIDALVALVDSPDMTIKQLMRHIPGPDF
                     PTGGFIHGRDPIIRAYHEGKGIIQMRGKAFTETVKRTGKEQIIIREIPYQVNKKRLIE
                     QIAELVNEKKIEGIGDLRDESDREGMRIVIELKRDAVAEIIINQLHKHTQLQESFGMN
                     MLAIVDGKPKLLTLKEALKCFLDHRKEVVTRRTAYDLRKAEERLHILEGYRIALDNLD
                     AVISLIRNSADGKAAKEGLMTQFALSELQAQAILDLRLQRLTGLERDKIMEEHRETVE
                     LIAKLRAILADEKEIFKIIVGELKDIKKKYGDERRTQIVDHSEDISIEDMIVDEDMAV
                     TISHEGYIKRNPVTLYRAQRRGGKGKIGTTTKEEDFVESLFIASMHSYILFFTTVGKV
                     YWIKVHELPQASRAARGRPIVNLLNLEPGEKVSAFLTVREFQEGRYIIFGTKNGL"
BASE COUNT         1175 a         1198 c         1288 g          968 t
ORIGIN      
        1 ataagactgc gcacggcgtc agttgtggct tagtagacgg gtagagtcaa tcactgcgaa
       61 tcgcaaaaac ttgtcaactt agggcagatt ctaccggacc ggaaaaaaac ctaaaaatcg
      121 ctgagaattc aatgatttat agcctcgacg gtgctttact ttcaggcgct gttccggtac
      181 tattcctgta gctgaaattc tgcctaaccc cagaattttt ctcactaaat tcaaagtccg
      241 acgataggtg aaaatggctg acgaaaaaaa tccaccagtg gcggaatatg gcgccgacaa
      301 cattaaagtt ttggagggtc tggaggccgt gcgcaagcgt cccggtatgt atatcggcga
      361 caccagtgag cgcggtttac accatctcgt ttatgaggtt gtcgataatt ccatcgacga
      421 agcgctcgct ggtcattgca acagcatcca ggtccaaatc cacatcgata acagcgttac
      481 cgtcattgac aatggccgcg gtatacctgt cgacattcat cccacggaaa aaatttccgc
      541 ggccgaagta gtgctcacca agctccacgc tggtggcaaa ttcgataaaa attcctacaa
      601 agtgtccggt ggattgcacg gggtcggcat ctccgtggtc aacgcccttt cggaaacgct
      661 ggaaattgaa attaagcgcg acggcaaggt ttaccagcag agttaccggc gcggcgatcc
      721 gcaggggcct ttgaaggaag tcggcaagtc caaagagcgc ggcacgcggg tgaccttcaa
      781 accggatacg cagatcttcg aggtgagcga atttagcttc gatattttgt cgcagcgatt
      841 gcgcgaacta gcattcctca atcgcggctt aaaaattacc attgaagacg agcgcacgca
      901 aaaaaatcac gagtttttct acgaaggcgg gcttttatct ttcgtgcagc atcttaatcg
      961 tgccaagagt gcgattcatt caaaggttgt tcactgcgag ggggagaagg aaggcgtcgg
     1021 tgtcgagatc gcaatgcaat ggaacgatag ctacgccgaa aatgtttttt ccttcgccaa
     1081 caacatcaac acgattgagg gcgggacgca cctgatcggt tttcgctcag cgctgacgcg
     1141 ttcgatcaac aactatgcaa tcggcagcgg ccaacttaag aaggatgatg agaatctcca
     1201 gggcgatgac gtgcgcgagg gtttgaccgc agtcatcagc gtcaaagttc ccgagccaca
     1261 gttcgaaggc cagaccaaga ctaagttagg caacagtgaa gtcaaaggca tcgtcgaggc
     1321 tttggtcaac gaaaagttgg gcaattatct gagcgagcat cccgcggacg ccaaaaaaat
     1381 cgtcgccaag ggcgtcgaag ccgcgcgcgt gcgcgaggcg actcgcaagg ccaaggatct
     1441 ggcgcgtcgc aagggggcac tcgattcggg ttctctacct ggcaagctcg ccgactgcca
     1501 agagcgcgat ccggcgctga gcgaactcta cctggtcgaa ggtgattccg ccggcggttc
     1561 ggccaagcag gggcgcgatc gccgcaatca agcgatcctg ccgctcaaag gcaaaatcct
     1621 caatgtcgaa aaggcacgct ttgataagat gctgtcgtct caagaaattc gcctgatgat
     1681 caccgctttg ggcactggcg ttaaggaaga ttacgaccaa gccaagctgc gctaccattc
     1741 gatcatcata atgaccgacg ccgatgttga cggttcacat atccgcacgc tgttgctgac
     1801 gttcttctat cggcagatgc ccgagctagt cgaaaagggc tatctcttca tcgcacagcc
     1861 gccgctgtat aagatcaaga agggcaaaca ggagcgctat cttaaagacg aggcgatgct
     1921 cgaggatcac ctgatcgaac tcggcacgga gaacgtgcga ctgcgcgccg cccaaaatgg
     1981 caacgctaac ggtaacggtt tggccgggca gcctttgcaa ggtttcgtca agaaggttgc
     2041 gcgctgggaa aagttgttaa tctccctcgg ccgcaaacgg aaaaaccgga ccgtggtcga
     2101 agcgttgttg ttggaagacg ggttcggcga ggagatgcta aaagatcgcg aggcgttgct
     2161 acggctggag ggccgcttgg cgacatatgt cggactgacc gcacccgagc agtcgcccgt
     2221 gtcctgcgct ctcgaagagg acgcggagca taattgcttc aagctggtcg ttaccacgcg
     2281 cgccaatggc accggttatc agaccgtcat tgatcgcgag tttctcacca ctccggaatt
     2341 tagagaaatt aagaagctgt tcggcgaaat ggccggcatc ggaatgccgc cttattcgat
     2401 cgaagatggc gacgacaaag cgcacctgaa atcattaggt gcgattcttt cttacgtcat
     2461 ggagggcggc aagaaggggc agtatattca gcgctacaag ggattgggcg aaatgaatcc
     2521 ggaacaactg tgggagacca ccatgaatcc cgatacgcgc gtgctcttgc aggtgcgtat
     2581 tgaggacgct gtggaagccg accagatctt ctcgaccctg atgggcgatg aagtcgagcc
     2641 gcgacgcaag tttatcgaag ataacgccct gacggtgcga aatttagata tctaacgcgc
     2701 tgtctgcagc gtttcttgat cggcatttta aaaaacaaat caagcagcta agttagcgaa
     2761 aaactcatgg aagctaccat caagccgcaa aaggttccgg tctacatcga agaagagatg
     2821 cgcgagtcgt tcatggccta tgccatgagc gtcatcatta gccgcgctct gccggacgtg
     2881 cgcgacgggc tgaagccggt gcatcggcgc gtgctttatg ccatgtacga cgccagcaac
     2941 acctcggaca agccgtacaa aaaatccgcc cgtctggtcg gcgatatcat gggtaagtat
     3001 catccccatg gcgacacggc gatttacgat accgtggtgc gcatggcgca ggacttcaac
     3061 ctgcgctatc cactggtgga cgggcagggc aactttggtt ccatcgacgg cgataatccc
     3121 gcggccatgc gttacaccga gatccgcatg acactgttgg ccgaggagat gctcgccgac
     3181 atcgaaaaag aaactgtcga ttttgttccc aattacgatg attcgctcaa ggaaccggcg
     3241 gttctaccgt cgcggattcc taatttactg atcaatggtt ctgccggtat tgcggtcggc
     3301 atggcgacca atatcccgcc gcataatctc aacgaagtga ttgatgcgct ggtcgccttg
     3361 gtggacagcc cagacatgac gatcaagcag ttgatgcgcc atattcccgg accggatttt
     3421 cccaccggtg gattcattca cggtcgcgac cctatcatcc gcgcatacca tgaaggcaag
     3481 ggcatcattc agatgcgcgg caaggcgttc accgaaactg tcaagcgcac gggcaaggaa
     3541 caaatcatca tccgcgaaat tccctatcag gtcaacaaaa agcgcctgat tgaacagatc
     3601 gctgagctgg tcaacgaaaa aaagattgaa ggcattggcg atctgcggga cgagtcggac
     3661 cgcgaaggca tgcgcatcgt tatcgagctc aaacgcgacg ccgtggccga gattatcatc
     3721 aatcagctgc ataaacatac ccagctgcaa gagtcattcg gtatgaatat gctcgccatc
     3781 gtcgacggca agcccaagtt gctgacgctc aaagaagcgc tcaagtgttt cctcgatcac
     3841 cgtaaggaag tcgtgacccg gcgcacggct tacgatctgc gtaaagccga agagcggctg
     3901 catattttgg aaggctatcg tatcgcgctc gacaacctcg atgcggtgat ctcgctgatt
     3961 cgtaactccg cggacggcaa agccgctaaa gagggcttga tgacccaatt cgccttgagc
     4021 gagttgcaag ctcaagcgat cctcgacctg cgcttgcagc gtttgaccgg tttggagcgc
     4081 gacaagatca tggaggagca tcgcgagacc gtcgagctga ttgcgaagct gcgcgcgatc
     4141 ctcgccgatg agaaagaaat attcaaaatc atcgtcggcg aactcaaaga cataaagaaa
     4201 aagtatggcg atgagcggcg cacccagatc gtcgaccact cggaagatat ttccatcgaa
     4261 gatatgattg tcgatgagga tatggcggtg acgatctcgc acgagggtta cattaagcgt
     4321 aatccggtga cgctctatcg ggcgcagcgg cgcggcggca aaggcaagat cggcaccacg
     4381 accaaggaag aggactttgt cgagtcgttg ttcattgcgt cgatgcactc ctatattctg
     4441 tttttcacta ctgtcggtaa ggtgtattgg atcaaagtcc atgaactgcc gcaggcgagc
     4501 cgggcggcgc gcggccggcc catcgttaat ctgctcaacc tcgagcctgg cgaaaaggtt
     4561 tcagcgtttc tcaccgtccg ggagtttcaa gaagggcgct atatcatttt cgggaccaag
     4621 aacggcttg
//
LOCUS       DATLCH010000119         4739 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1866177, whole genome shotgun sequence.
ACCESSION   DATLCH010000119 DATLCH010000000
VERSION     DATLCH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866177"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VMZ02_03485"
     CDS             <1..204
                     /locus_tag="VMZ02_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71023.1"
                     /translation="QFDRVGVELRDKIGIDNVMWGSDFPHQESDWPDSLKIIDKNFAG
                     VPQEERRRMVCDNAVEFFRLDSA"
     gene            215..1324
                     /locus_tag="VMZ02_03490"
     CDS             215..1324
                     /locus_tag="VMZ02_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR71024.1"
                     /translation="MFRCFVSILWVLCVVSARPSHSQDNWQTEWDRTVKLAEQEGQVV
                     YSGCGSHDYLKEFQKSFPKIKLTSVALSCTQLVSRIMAERRAGKYLGDVVRFGLTSAH
                     TFYRAKVLQPIDQALILPEVKDASKWWQNKLQYSDPEGRYLIVPAGSIYERFASYHSA
                     LASPSDFKSFADILNPKWKGKIVATDPNTGEGRNGARFLYHHAELGPKYLRRLLSEMD
                     ITYSRDYRQATDWLAQKRFALYLFSQSDDTLNAKSQGLPIQVLDTSNWKEAPGLDAIG
                     GAYLLMDKPAHPNAAKILVNWLLSRDGQIALQRDGENAGSIDSLRTDIPKSDVNPLMR
                     RKDGVKYLEMWNPNWMNMKPVQELIDQAMNEGRKS"
     gene            1455..2726
                     /locus_tag="VMZ02_03495"
     CDS             1455..2726
                     /locus_tag="VMZ02_03495"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HUR71025.1"
                     /translation="MAEPTVVCPQCKTEIKLTESLAAPLLADVKREFEQKLVQKDADM
                     AKREQSLRERAESVEKAKANLDQQIAAKLQQERIRIAAEEQQKAKLVLGNDLNQKTKE
                     ISTLQEVLKQRDAKLAEAQRAQAELIGKQRELDDARRELELTIAKRVQADLAGERERA
                     KREAEEELKFKVMEKDQTITAMQKQIEDLKRRAEQGSQQLQGEVQELELEALLTAKFP
                     RDMIQPVPKGEFGGDVLHRVVGPTNQPCGTILWESKRTKNWSDGWLPKLREDQRAAKA
                     EIAVIISQALPKDIETFGFTGEVWVADPKVALPVAMALRQTLIEVACLRQASEGQQSK
                     MEMIYGYLTGPRFRQRVQAIVEAFSSMKKDLDVEKKVITRQWAKREEQIDRVMQATVG
                     MYGDLQGIAGRTLQEIEGLEFQGMLDFDGDK"
     gene            2851..4371
                     /locus_tag="VMZ02_03500"
     CDS             2851..4371
                     /locus_tag="VMZ02_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HUR71026.1"
                     /translation="MSDHYDVIVVGGGSAGCVLAARLSEDSNRKVLLLEAGPDPQPIP
                     DTVADAEKTTYVLLESPYIQMYPTKRNYDGSEFYSLAGRITGGGSSVNMMAIPRPIKA
                     DLDIWASQGNPEWSWDKVRPVLRRMEADQDFPNDPIHGKSGPIYIKRKHLFDIELGEQ
                     ENALLETLDKLGIPRFLDQNIPNPSGIAPVARNSRDGKRQSATIMYLDPARPRANLTI
                     IDEAPATSLLLNGKRVTGVRYRKAGQDHSVSADKVVLSAGVYHSPQIMMLSGIGPSAQ
                     LKQHDIPLAHDMPGVGENYQDHPMLTMTFKTNPANTKPLMRGRSSLKLYFKTDPAREY
                     LNFHIIPREAISISGIGDMLGFSCNLLEQTNRGKLTLQSADPDDLPSIDPQVLEHPKD
                     IAAMLAAMKFVQRLTTSEPLSQFFGELFSPGVHEDWEKFARSSYTSYYHGVGTCKMGP
                     ASDPMAVVDQHLRVHGFDNLWVGDASIMPTVVHANTNFTVMMIAERAAEFIKASSR"
     gene            4384..>4739
                     /locus_tag="VMZ02_03505"
     CDS             4384..>4739
                     /locus_tag="VMZ02_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUR71027.1"
                     /translation="MARIVIETKIRAPIELCFDLARDVDAHRESAAFSAERVVPPGRT
                     EGLLELGDLVAFEGRHFGIRQRFVARITKLDRPHRFDDEMVEGSFKWLRHYHEFSFSN
                     GITTMRDTLEWQPPLG"
BASE COUNT         1160 a         1348 c         1300 g          931 t
ORIGIN      
        1 caattcgacc gtgttggggt cgagctgcgc gacaagatcg gcatcgacaa tgtcatgtgg
       61 ggctctgatt tcccgcatca agagtccgac tggcccgact cgctcaagat catcgataaa
      121 aacttcgccg gcgtgccgca agaagaacga cgtcgcatgg tctgcgataa cgcggtggag
      181 tttttccgac tcgactcggc atgagcagct ggtgatgttt cgctgcttcg tttcgatcct
      241 atgggtccta tgcgtcgtct ccgcccgtcc atcgcactca caagacaact ggcaaacgga
      301 atgggaccgc accgtcaaac tcgccgaaca ggaaggccaa gttgtctact ccggctgcgg
      361 cagtcacgac tatctcaaag agtttcagaa aagcttccct aaaattaagt taaccagcgt
      421 cgcgctttcg tgcacacagc tggtgtcgcg gatcatggcc gagcgccgcg ccggcaagta
      481 tcttggcgac gtcgtgcgct ttggcctcac ttcggcgcat accttctacc gcgccaaggt
      541 gcttcagccc atcgaccaag cactgatcct gccggaagtg aaagacgcgt ccaaatggtg
      601 gcaaaacaaa cttcaatact cagacccgga aggcagatat ttgatcgtcc cggcaggcag
      661 catctacgag cgcttcgcca gttatcattc ggcgctggcg agtccgagcg acttcaaatc
      721 tttcgctgac attttgaatc cgaaatggaa aggcaagatc gtcgccaccg atcccaatac
      781 cggcgaaggc cgcaacggcg cgcgttttct ttaccaccat gccgagctcg gaccgaagta
      841 tctgcgccgg ttgctgagcg agatggatat cacctactca cgggattatc gccaggcgac
      901 cgactggctg gcgcagaagc gtttcgcact ttacctcttc agccaatccg acgacacgct
      961 caacgccaaa tcccagggac taccgattca ggtcctcgac acgagcaact ggaaggaagc
     1021 gccgggactc gacgccatcg gtggcgctta tctgctgatg gacaaaccgg ctcatcccaa
     1081 tgccgcaaag attctcgtca attggctgct ctcgcgcgac ggtcagatcg ccttgcagcg
     1141 cgacggcgaa aacgccggaa gcattgattc cctgcgtacc gacattccga agagtgatgt
     1201 aaatccgctc atgcgccgta aagacggcgt caaatatttg gagatgtgga atccaaactg
     1261 gatgaacatg aagccggtgc aggagctcat cgatcaggcg atgaacgaag gcagaaaatc
     1321 ctgacgcaac ttctcctttt caactactgc aacttctaat ctgccgcgcg catccctttg
     1381 cactttggcc tgatttggtt actctcatcc caaaccaaca cgttacgaaa gacgaacacg
     1441 aactggagtc cttcatggcc gaaccgacag tcgtatgtcc gcagtgcaag actgaaatca
     1501 aactgacgga atctcttgcc gcgccactgc tggccgacgt aaagcgggag ttcgagcaaa
     1561 agctcgtaca aaaagatgcc gatatggcga aacgcgagca atcgttgcgt gagcgcgccg
     1621 agtccgtcga aaaggctaaa gcaaatcttg atcaacagat tgccgcgaag ctccaacaag
     1681 aacgcatccg catcgccgcc gaagagcagc aaaaagccaa gctggtcctg ggcaacgatc
     1741 tcaatcaaaa aactaaagag atcagcacgc tgcaagaagt attgaaacaa cgagatgcca
     1801 agcttgccga agcgcagcgg gcgcaggcgg aactgatcgg caagcagcgt gagttggatg
     1861 acgccaggcg cgagctggaa ttgacgatcg ccaaacgggt gcaagccgat ctcgccggtg
     1921 agcgtgagag agccaagcgc gaagctgaag aagaacttaa attcaaagtg atggaaaagg
     1981 accagaccat cacggcgatg caaaagcaga tcgaagattt gaaacgacgc gccgaacagg
     2041 gctcgcagca acttcaagga gaggttcagg aattggagtt ggaggcattg ttaacggcaa
     2101 aatttccgcg cgacatgatt caaccggtgc ctaaaggcga attcggcggc gacgttttgc
     2161 accgggtcgt cggtccgacc aaccagccgt gcggcacgat cctatgggaa tccaagcgga
     2221 cgaaaaattg gagcgacggc tggctgccca agctgcgcga agaccagcgt gccgccaaag
     2281 ccgagatcgc cgtgatcatt agccaggcct tgccgaaaga tattgaaact tttggtttta
     2341 ccggcgaggt ttgggtcgcc gatcccaaag tcgccctgcc agtggccatg gcgctccgcc
     2401 aaacactgat cgaggtcgca tgcttgcgcc aagcatccga aggtcagcag agcaaaatgg
     2461 aaatgatcta cggctacctc accggaccgc gctttcgcca gcgagttcaa gcgattgtcg
     2521 aagctttctc gtccatgaag aaggatttgg acgtcgagaa aaaggtcatc acccgccaat
     2581 gggccaagcg cgaagaacaa atcgaccggg tcatgcaagc gaccgtcggc atgtatggcg
     2641 acttgcaggg catcgccgga cgaacccttc aggagatcga aggattggag tttcagggga
     2701 tgttggattt tgacggggat aaatagccgg cttcgacgac tcacgacctc ggaacccaat
     2761 gagatattat agttttcgaa aagctccgaa cgttttgaac ggagcgcagc gattcaaaca
     2821 acttaaatct actagtctta gggattcacc atgtctgatc attacgacgt gatagttgtc
     2881 ggcggcggtt cggctggctg cgtattggca gcgcgtttgt cggaggactc gaatcgcaaa
     2941 gttttactgc tcgaagccgg cccggacccg cagccgattc ctgataccgt cgccgatgcc
     3001 gaaaagacga cttacgtttt gctcgagagc ccttacatcc agatgtatcc gaccaagcgc
     3061 aattacgacg gcagcgagtt ctattcgttg gccggccgca tcaccggcgg cggatcgtcg
     3121 gtcaatatga tggcgatccc gcggccgatt aaagccgatc tcgacatttg ggcgagccag
     3181 ggcaatcccg aatggtcatg ggacaaagtc cgaccggtgt tgcgacgcat ggaagccgat
     3241 caagattttc ccaacgaccc gatccatggc aaatcggggc cgatttacat caagcgcaaa
     3301 catcttttcg atatcgagct tggcgagcag gagaacgcct tgctcgagac gttggacaag
     3361 ctcggcattc cacgattctt ggaccaaaac attccgaatc cttccggcat tgcaccggtc
     3421 gcgcgcaaca gtcgcgacgg caagcgccag tcggcaacga tcatgtatct cgatccggcg
     3481 cgcccacgag ccaatctgac gatcatcgat gaggcgccgg cgacctctct cctcttaaac
     3541 ggtaaacgcg tgaccggcgt gcgctatcgc aaagcgggtc aggaccacag cgtcagcgcc
     3601 gataaggtgg tgctcagcgc cggcgtctat cattcgccgc agatcatgat gctgtcgggc
     3661 atcggcccga gcgcgcaact gaagcagcat gacattccac tggctcatga catgcccggc
     3721 gtcggcgaaa actatcagga tcacccgatg ctgaccatga cgttcaaaac caacccggcg
     3781 aataccaagc cgctgatgcg cggcagatcg agtttgaagc tctactttaa aaccgatccg
     3841 gctcgcgagt atctcaactt ccacatcatt ccccgcgagg cgattagtat ctccgggatc
     3901 ggcgacatgt tgggcttttc gtgcaatctg ttggaacaga ccaatcgcgg caagctcacg
     3961 ctgcaaagcg ccgaccccga cgatctgcca agtatcgatc cgcaggtctt ggagcatccg
     4021 aaagacattg cggcaatgct ggcagcgatg aaattcgtcc agcgcctgac caccagcgag
     4081 ccgctgagtc agttcttcgg cgaactcttt tcgccgggcg tccacgagga ttgggaaaag
     4141 ttcgcgcgct cctcgtacac gagctactac catggcgtcg gcacctgcaa gatgggtcca
     4201 gcgtccgatc cgatggccgt ggtcgatcaa cacctgcgtg tgcatggctt cgacaatctc
     4261 tgggttggcg atgcgtcgat catgcccacc gtagttcatg ccaatacaaa ttttaccgtc
     4321 atgatgatcg ccgaacgggc ggccgagttc atcaaggctt catcgcgata gagagaagca
     4381 accatggcgc gcatcgtcat cgagactaag attcgcgcgc ccatcgaact gtgctttgat
     4441 ctcgcccgcg atgtagatgc ccatcgagaa tcagcggcgt tttccgcgga gcgagttgtg
     4501 ccgcccggac gaactgaagg tttacttgaa ttgggcgatc tggtcgcctt cgaaggccgc
     4561 cactttggca tcaggcaacg tttcgtcgcg cgcatcacca aactcgatcg gccgcacaga
     4621 tttgacgacg agatggttga aggcagcttc aaatggctgc gccattatca cgaattctcg
     4681 ttcagcaacg gcatcaccac catgcgggac acccttgaat ggcaaccgcc gctaggcat
//
LOCUS       DATLCH010000120         4203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1122925, whole genome shotgun sequence.
ACCESSION   DATLCH010000120 DATLCH010000000
VERSION     DATLCH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122925"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VMZ02_03510"
     CDS             complement(<1..224)
                     /locus_tag="VMZ02_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71028.1"
                     /translation="MSRPEPIQREKPDVVPPISLKVENTGLDFGFLADLALKIVYSDA
                     NCTTERAADKMKLAMPVVEELLQHLYREKFL"
     gene            436..657
                     /gene="rpmE"
                     /locus_tag="VMZ02_03515"
     CDS             436..657
                     /gene="rpmE"
                     /locus_tag="VMZ02_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HUR71029.1"
                     /translation="MKAGIHPEYKPAKVTCACGSITETRSTAGNLHVEICSNCHPFFT
                     GKQKLVDTAGRVEKFRKKYGMQNQNTAEG"
     gene            819..1874
                     /gene="prfA"
                     /locus_tag="VMZ02_03520"
     CDS             819..1874
                     /gene="prfA"
                     /locus_tag="VMZ02_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HUR71030.1"
                     /translation="MLDKLLEVEKRYIELEGMMSDPQLVGQQREYAKVAKERSDLEEI
                     VVCYREWRKVEQEIANNRDLMDENDEAMRELVKEELMSLRERKEALETKLKFLILPKD
                     PNDERNVIVEIRAGTGGDEAALFAAELFRMYSRYAESRAWRVEMMSSNPTGLGGYKEI
                     IISIEGRGAYSRLKFEGGVHRVQRVPVTEGSGRIHTSAVTVAVLPEADEVEIAIDPKE
                     IRIDVFRSSGPGGQSVNTTDSAVRITHIPTGIVISCQDEKSQHKNRAKGMKILRARLL
                     EKKQDEQASEIAATRKLMVGSGDRSERIRTYNFPQGRLTDHRINLTIYQLERVMEGQI
                     DEVVEALITHYQAAALK"
     gene            1888..2793
                     /gene="prmC"
                     /locus_tag="VMZ02_03525"
     CDS             1888..2793
                     /gene="prmC"
                     /locus_tag="VMZ02_03525"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HUR71031.1"
                     /translation="MDVNITNRVGAPRLHGELILAFERLSRGGIENPRLDAEVLLARC
                     LNLSREDLLVAADLPISPDASSCFETFLQRRLTREPVAYITGTQEFWSLDFTVSPDVL
                     IPRPDTERLVEIALLWAARFAPSHALRIADLCTGSGAVAVSLASELPSARIWATDNSA
                     AALAIARGNASAHLVAAQIEFVTGDLFDALAPVLDEGFDLIVSNPPYVRRAEIATLAP
                     EVSRWEPRAALDGGVDGLDFYRRIIAAAPDYLAEHGALALEIGADMAIEVSAIFASTR
                     RYREIDTFQDYAGRNRVMLAQLRNT"
     gene            2806..4074
                     /gene="murA"
                     /locus_tag="VMZ02_03530"
     CDS             2806..4074
                     /gene="murA"
                     /locus_tag="VMZ02_03530"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HUR71032.1"
                     /translation="MDKILIKGGKTLNGEIAVSGAKNAALPILISSLLTAETCTYRSV
                     PHLADIRTTLKLLAGFGGKGDHQRWRDGADDLSLQADDIINFEAPYDLVRTMRASFLV
                     IGPLVARFGRARVSTPGGCAIGARPIDLHLKGLEALGATIEQTHGYVEARATKLRGAK
                     IYLDLPSVGATENLMMAASLAEGVTVIENAAKEPEIDDLAKALNAMGGQVHGAGTDVI
                     QIDGVAALHGLDHRIIPDRIEAGSFIIAAALTGAKVYVKGARADHLEAFLIKLREAGV
                     ACNSDNGGIFVCGCDEIKSVDVKTQPYPGFPTDLQAQMMVLMAVADGVSVITETIFEN
                     RFMHVPELDRMGADIKLEGNRAVVRGVKEHSGEPVMASDLRASVALVLAGLVATGSTE
                     VARIYHLDRGYEQIEKKLSYLGAQIERTAG"
BASE COUNT          955 a         1072 c         1232 g          944 t
ORIGIN      
        1 agaaactttt cccgatagag gtgctggagc agttcctcga ctaccggcat ggctaatttc
       61 atcttgtcgg cggcgcgctc ggtggtacag ttggcatctg aatagacaat tttaagtgcc
      121 agatcggcga ggaacccgaa atcgaggccg gtgttctcga ctttgagcga gatcggcggc
      181 actacgtcgg gcttctctct ttgaattggt tctggcctgc tcatggtttg cctgtccttt
      241 tcacctggct ggtgattgag gtcataataa caattagctg attttattcg acgtatttta
      301 tgggcttacg gtctttttgg caatcgattc tatcaagcgt caaaatgtgg cgaatcgctc
      361 aatcaatctt ggcgggtatg ccaattgaag gtctccagac gatgtgttat ataactcgtc
      421 cattgagagg tacacatgaa ggcaggaatt catccagaat ataagccagc caaggtcact
      481 tgcgcctgcg gaagtattac cgagacgcgc tcaacagcag gtaacctcca cgtcgagatt
      541 tgctccaact gccatccgtt ctttacgggt aagcaaaagt tggtcgacac tgccggacgg
      601 gtcgaaaagt tccgcaaaaa atacggcatg cagaaccaaa atacggcgga gggctgaggc
      661 cgatttgacc aattagtttt acgcaaactc caaagggcaa agggcaggtt cgcaaccctt
      721 tgcccttttt tatttcgcct gcaaccaatc gccgaagcgg gaaacttctt tgatcgtcaa
      781 tggcatcaat tatccaactc cggaagtgac gcgcagttat gcttgataaa ttactggaag
      841 tcgaaaagcg ctacatcgaa ctcgaaggca tgatgtccga cccgcagttg gttggtcaac
      901 aacgcgagta tgccaaggtc gccaaggagc gctccgattt ggaagagatc gtcgtctgct
      961 accgtgagtg gcgcaaagtc gagcaagaga ttgccaacaa ccgcgacctc atggatgaaa
     1021 acgatgaagc gatgcgcgag ttagttaagg aagagttgat gagcctacgt gagcgcaaag
     1081 aggccctcga aaccaagctc aagtttctca ttttgccgaa ggatcccaac gacgagagga
     1141 acgttatcgt tgaaattcgc gctggtactg gcggtgatga ggcggcgcta ttcgccgccg
     1201 agctgttccg tatgtactcc cggtacgccg agtcgcgcgc ttggcgggtc gagatgatga
     1261 gctcaaaccc tactggtttg ggtggctaca aagaaattat catcagcatc gaaggccggg
     1321 gcgcctatag ccgactcaag ttcgaaggcg gtgtgcaccg ggtgcaacgc gtccccgtca
     1381 ccgaaggatc gggacgcatt catacgtcgg cagtcaccgt ggcggtgttg ccagaagccg
     1441 atgaggtgga aatcgccatc gatccgaaag agattcgcat agacgtcttt cgctcatcgg
     1501 gtccgggcgg ccagagcgtt aacaccaccg attcggcggt acgcatcacg catattccga
     1561 cagggatcgt aatttcctgt caagacgaaa aatcgcaaca taagaatcgc gccaaaggca
     1621 tgaagatttt gcgcgcgcgc ttgttggaaa agaaacagga cgagcaggca tcggagatcg
     1681 ccgcaacaag aaaattgatg gtcggcagcg gcgaccgcag cgagcgcatc cggacgtata
     1741 attttccgca ggggcgattg accgatcatc ggatcaattt gacgatctac cagcttgagc
     1801 gtgtgatgga aggacagata gatgaggtgg tcgaagcatt aatcacccac taccaggcag
     1861 cggcgctcaa gtaagcacaa tgcagcgatg gacgtgaata taactaaccg cgtgggcgcg
     1921 ccgaggttgc atggcgagct gatactcgct ttcgagcgtt tgtcccgcgg cgggattgaa
     1981 aacccgcgcc tggatgctga agttttgctg gcacgttgtt tgaatctgag ccgcgaggac
     2041 ttgcttgttg ctgctgattt accgatatct cccgatgcct cgagctgctt tgagactttc
     2101 ttacaacggc gtttaactcg ggaaccggtg gcttacatta ccggcacgca ggagttctgg
     2161 tcgctcgact tcacggtttc tccggatgtg ttgataccac ggccggatac cgaacgccta
     2221 gttgaaattg cgctgttatg ggcagcgcgc ttcgcccctt ctcacgcgtt gcggattgcc
     2281 gacttgtgca ctggcagcgg tgcggtagcc gtcagtttag caagtgagtt gccgtccgcg
     2341 cgcatttggg cgacggataa ttccgctgcc gccttagcga ttgcccgcgg caacgcctcg
     2401 gcgcatcttg tggcggcgca gatcgagttc gtcacgggcg atctgtttga tgccctcgcg
     2461 cctgtactag atgaaggttt cgatttgatc gtcagtaatc cgccgtatgt gcgtcgcgcc
     2521 gagattgcta cgctggcacc ggaagtgagc cggtgggagc cgcgtgcagc tttggacggc
     2581 ggcgtcgatg gacttgattt ttatcggcgc atcatcgctg ccgctccgga ttatttagcc
     2641 gagcatggag cgctggcgct ggagatcggc gccgatatgg caatcgaggt ttcggcgatc
     2701 tttgcgagta cccgacgtta ccgggagatc gacacatttc aggattatgc cggacgcaat
     2761 cgtgtgatgt tggcgcaact gcggaacact taaggaaccg cggaaatgga caagattctc
     2821 ataaagggcg gcaaaacttt gaatggtgag atcgccgtta gcggtgctaa gaacgcggcg
     2881 ctgccaatac tgatctcatc gctgctcacc gccgagacct gtacctatcg aagcgtgccc
     2941 catctcgctg atattcgaac cactttgaaa ttgctcgcag gtttcggcgg caagggcgac
     3001 caccaacgtt ggcgcgatgg cgccgatgat ttgagcctgc aagccgatga catcatcaac
     3061 tttgaagcgc cttacgattt ggtcaggact atgcgagcgt cgtttttggt cataggtccg
     3121 ctggtggcgc gcttcggccg ggcacgggtg tcgacacccg gcggttgcgc catcggcgcg
     3181 cggcccatcg atttgcattt aaagggcttg gaagccctcg gtgcgacgat cgagcaaacc
     3241 cacggttatg tcgaagctcg cgcgacgaaa ctgcgcggcg caaagattta tttagacttg
     3301 ccatcggtgg gcgccacgga aaatctcatg atggcggcat ccctcgccga aggtgttacc
     3361 gtcattgaaa atgccgccaa agagccggag atcgacgacc tggcgaaggc gctcaacgcc
     3421 atgggtgggc aagttcacgg cgcgggaacc gatgtgattc agatcgacgg tgttgccgct
     3481 ttgcatgggc tagatcaccg aatcattccc gaccgcattg aagccggaag ttttatcatc
     3541 gccgctgccc tcaccggcgc taaggtctac gtcaaaggcg cacgcgccga tcatctcgaa
     3601 gcttttttga ttaaattgcg tgaagccggc gtagcgtgca atagcgataa tggcggcatc
     3661 tttgtgtgcg gttgcgacga gattaaaagc gtcgatgtga agacccagcc ctatcccggt
     3721 tttcccaccg acttacaggc gcagatgatg gtgttgatgg cggttgccga tggtgtgagc
     3781 gtgatcaccg agacgatttt tgagaatcgg tttatgcacg tgcccgaact cgaccgcatg
     3841 ggcgccgaca tcaagctcga gggcaatcgc gcggtggtac gcggcgtgaa agaacactcc
     3901 ggcgagccgg tgatggcgag tgatttgcgc gccagcgtag cactagtgct cgcgggtctc
     3961 gtcgctaccg ggagcaccga agtagcccgg atctaccacc tcgatcgcgg ttacgaacaa
     4021 attgagaaaa aactatctta tttgggcgcg cagattgaac ggactgccgg atgagattgt
     4081 ggaaggctca cggattcaaa ggttcaaggt cggaaatttc actgccgcag acaaaatgaa
     4141 cattcgttta gtaaggactt cggacgccgg atttaagccg ttattggcca agatcctcgg
     4201 gcg
//
LOCUS       DATLCH010000121         3036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7639226, whole genome shotgun sequence.
ACCESSION   DATLCH010000121 DATLCH010000000
VERSION     DATLCH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7639226"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /gene="argH"
                     /locus_tag="VMZ02_03535"
     CDS             complement(<1..1211)
                     /gene="argH"
                     /locus_tag="VMZ02_03535"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HUR71033.1"
                     /translation="MSVETYGYLKKNNKKLWGGRFSGGTADSVEAFTASIAIDSRLYR
                     HDIMGSIAHAKMLGKQRIIPLRDSQKIVRGLAAIQREIESGKFEFSPVDEDIHMNIER
                     RLTEKIGAAGGKLHTARSRNDQVALDMRLYLRDAVATILVSLEALQRELTKAAKKHLD
                     VIMPGYTHLQRAQPVLLSHHLLAYHEMFARDHERYLSCLERVNVLPLGSGAIAGTTFP
                     IDRGYVAKLLGFRRISKNSIDAVSDRDFLLEFLSVSSILFVHLSRMAEELVLWSSQEF
                     AFIELPDAYCTGSSMMPQKKNPDVPELIRGKSGRVFGHLLALLTIMKGLPLAYNRDLQ
                     EDKVPLFDCADTVQASVTIMGEIVGGLKVRNERMLAAAQEGYLNATDLADYLVERGVP
                     FRQAHEAAGRIV"
     gene            complement(1268..2467)
                     /locus_tag="VMZ02_03540"
     CDS             complement(1268..2467)
                     /locus_tag="VMZ02_03540"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HUR71034.1"
                     /translation="MNVKKVVLAYSGGLDTSVILRWLKEEYGCEVIAFCADLGQGEDL
                     QAVRKKAIQTGASKVVIGDLREEFVANYVFPMLRANAVYEGSYLLGTSIARPLIAKGQ
                     IDIAVREKADAVSHGATGKGNDQVRFELTYYALKPGIKIIAPWREWNLNARSTLIDYA
                     RKHGIPVPVTKAKPYSTDRNLFHISYEGGVLEDPWKEPPSDMFVWCRPLEKAANRAEY
                     IEIDYLRGDPVVLNGKRLSPANLLAQLNAIGAKHGIGRVDLVENRYVGMKSRGVYETP
                     GGTILHAAHRGLESITMDREVLHLRDSLIPRYAEMIYYGYWFAPERELLQKTIDGSQA
                     NVTGRVRLKLYKGNVMVVGRKADASLYDANVATFEADQVYRQADAEGFIRLNALRLRL
                     RRLAKAK"
     gene            complement(2491..>3036)
                     /locus_tag="VMZ02_03545"
     CDS             complement(2491..>3036)
                     /locus_tag="VMZ02_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71035.1"
                     /translation="ADCQTLIEHKGGLEGLKIVFVGDGNNMVHSWLEAAAIVPFSFIL
                     ACPKGYEPDTAIVQRARAHGAKIAVTHSVEEAVAGADAIYTDVWTSMGQEKQAQDRLS
                     AFRAYQVNSQVVAMAKKNAVVMHCLPAHRGEEITDEVLESSQCVAFDQAENRLHAQKA
                     VMVWLVNGSKASRVKNSTKKS"
BASE COUNT          660 a          881 c          789 g          706 t
ORIGIN      
        1 acgattctgc ccgccgcctc atgggcttgg cggaagggca cgccgcgttc gaccagatag
       61 tcggccaagt cggtggcgtt cagatagcct tcctgggccg ccgctagcat gcgctcgttg
      121 cggactttca atccaccaac aatttcgccc atgatcgtca cgctcgcctg caccgtgtcg
      181 gcgcaatcga acaacggcac tttatcttct tgcagatcgc gattataggc aagcggtaac
      241 cccttcatga tggtcaatag cgcaagcaga tggccgaaca cacgcccgct cttgccgcgg
      301 atcaactcgg gaacatcggg attttttttc tgcggcatca tcgaactgcc ggtacaataa
      361 gcatccggca gctcgataaa agcgaactct tgactcgacc agaggaccaa ctcttcagcc
      421 atccggctca aatgcacgaa caggatcgag gagaccgaga gaaactccaa caagaaatcg
      481 cgatcgctca ccgcatcgat gctgtttttc gagatccgcc gaaagcccaa taatttggca
      541 acgtagcctc tatcgatggg aaaggtcgtg ccggcaatgg cgccggagcc cagcggcaga
      601 acattgaccc gttccaaaca gctgagatag cgctcgtggt cgcgagcgaa catctcgtgg
      661 taagcgagca aatgatgcga tagcagcacc ggctgggccc gttgcagatg ggtatagccc
      721 ggcatgatca catcgagatg tttcttcgcc gccttggtga gttcccgttg cagcgcctcg
      781 aggctgacga gtatggtcgc caccgcgtca cgcaaataga gccgcatatc cagagcgact
      841 tgatcgttgc ggctgcgggc tgtatgtaac ttgccgccgg cggcgccgat cttctcggtc
      901 aaacggcgct cgatattcat gtgtatgtct tcatccaccg gcgaaaactc gaacttgccg
      961 ctctcgattt cgcgctggat ggccgccaga ccgcggacaa ttttttgtga gtctcgcaga
     1021 ggaataatgc gttgcttgcc gagcatcttg gcgtgagcaa tgctgcccat aatgtcgtga
     1081 cggtacagcc gtgaatcgat ggcgatggat gcggtaaacg cctccaccga atcggcggtg
     1141 ccgccgctga acctgccacc ccaaagtttt ttattgttct tcttcaagta accgtacgtc
     1201 tcgacgctca atggaaattg acatgatcgg atccggaatg atcaattctg ccttatccag
     1261 tttcaattta tttcgccttc gccaaccgcc gtaagcgcaa gcgtagagca ttcaaacgta
     1321 taaatccttc ggcgtcggct tggcggtaga cctgatcggc ctcgaaagtg gcgacgttgg
     1381 catcatagag ggacgcatcg gccttgcgtc caacgaccat gacgttgcct ttgtacaact
     1441 tgagccgtac ccgcccggtt acgttggctt gggagccatc gatagttttt tgcagcaact
     1501 cgcgttccgg cgcaaaccaa taaccgtagt aaatcatctc cgcatagcgt gggatgagag
     1561 aatcccgcaa gtgcagcacc tcgcggtcca tagtaatcga ctccaagcct cgatgcgccg
     1621 catggagaat tgtcccgccg ggagtttcat acacgccgcg tgacttcatg ccgacatagc
     1681 ggttctcaac caaatccacg cggccaatgc catgcttggc gccgatagcg ttcaattgtg
     1741 ccaacaaatt tgccggcgag agtcttttac cgttgagtac caccggatcg ccgcgcagat
     1801 aatcgatctc gatgtattcg gcgcggttgg ccgccttctc cagcggccga caccagacaa
     1861 acatgtccga agggggctcc ttccacgggt cttccaatac gccgccttcg taactgatgt
     1921 gaaataggtt tcgatccgtg ctgtagggtt tggccttggt gacaggcacg gggatgccat
     1981 gcttgcgcgc atagtcgatc aaagtcgagc gcgcattcag gttccattca cgccaagggg
     2041 cgataatttt gatgcccggc ttgagcgcat agtaagtcag ctcaaagcgc acctgatcat
     2101 tgcccttgcc agtcgcgcca tgagagaccg cgtcggcttt ttcgcgtacg gcgatatcga
     2161 tctggccttt ggcgatcagc ggccgagcaa tcgaggtgcc caataaatag gatccttcgt
     2221 atacggcgtt ggcgcgtaac attgggaaaa cgtaatttgc cacgaactct tcgcgcagat
     2281 cgccgatcac cactttactt gcgcccgtct gaatcgcttt cttgcggacc gcttgcagat
     2341 cttcgccctg gccgagatcg gcgcagaacg cgataacttc gcagccgtat tcttctttga
     2401 gccatcgtaa tatgaccgac gtatccaagc cgccggagta agccaatact acttttttaa
     2461 cgttcatcgg gatttagtgg gacctctgcg tcagctcttc ttggttgaat ttttaactct
     2521 cgatgctttc gatccgttga ccagccacac catcaccgcc ttctgcgcat ggaggcgatt
     2581 ctccgcttgg tcgaaggcga cgcactgcga gctttctaac acttcgtcgg taatctcttc
     2641 cccccgatga gctggcaggc aatgcatcac caccgcattc ttttttgcca tggcaacgac
     2701 ttgactgttg acctgataag cgcggaacgc gctcaagcgg tcttgcgcct gtttttcctg
     2761 gcccatgctg gtccaaacat cggtatagat cgcatcggct ccggccaccg cttcttcaac
     2821 tgaatgagta acggcgattt tcgccccatg agcgcgcgcc ctttgaacga ttgccgtatc
     2881 cggctcataa cctttcggac aggctaagat gaacgaaaat ggcacgatgg cagcggcttc
     2941 caaccatgag tgaaccatgt tgttaccgtc accgacaaag acaattttta agccctctag
     3001 cccgccctta tgttcaatca atgtttgaca gtcggc
//
LOCUS       DATLCH010000122        12393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8386014, whole genome shotgun sequence.
ACCESSION   DATLCH010000122 DATLCH010000000
VERSION     DATLCH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8386014"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VMZ02_03550"
     CDS             complement(<1..837)
                     /locus_tag="VMZ02_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71036.1"
                     /translation="MAGLNLHQVQKLAQTLVMTPQLQQAIKLLQLSRVELEEMVLKEL
                     QENPALEEGANDEPEVAEQPRAETKSEPTEEFVELVAPREPNTVDKIGTLDWQEYLDS
                     HSNGVHSSLTAEAMSDDGDSPPSWENSLTKKTSLEDHLIWQLRLSKISERESNIGFYI
                     IQNINENGYLALSVEEICNAVECAAEEVEAVLRRIQFFDPVGVAARNLRECLLVQLEN
                     LKLTDSLAARVVDGHLSYLESKRYEKLAKDLSVTVDDIVDAAHLIASLEPKPSRGFEQ
                     DEV"
     gene            complement(840..1562)
                     /gene="lptB"
                     /locus_tag="VMZ02_03555"
     CDS             complement(840..1562)
                     /gene="lptB"
                     /locus_tag="VMZ02_03555"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HUR71037.1"
                     /translation="MSVLKAVSVAKSYGGRRVVNGVNLVVQSGEVVGLLGPNGAGKTT
                     TFHMMVGLVQPDEGQVLLNEDNLTEAPIYQRARAGISYLPQESSVFRRLTVAQNLLAI
                     LQTLDLSSAEQKERADALMKMLGVSHLATHKAFTLSGGERRRVEIARALVLSPFFLLL
                     DEPFTGIDPIAVAEIQKIVRNLIISGIGILITDHNVRETLGICDRAYIVNEGTILEEG
                     TPQSIAASPRARKFYLGEGFKF"
     gene            complement(1559..2119)
                     /gene="lptA"
                     /locus_tag="VMZ02_03560"
     CDS             complement(1559..2119)
                     /gene="lptA"
                     /locus_tag="VMZ02_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HUR71038.1"
                     /translation="MIKKAFSTMILGLCLSVSFPSVYAVAAAAAGEGKTTSNAFEFNK
                     KDPIYITADWMEVDQAKNTIVYKGRVIAVQSDMTMRSDVLTAYYDPEMKQMKQIVLDG
                     KVNATQGTRVATGDRAIFDDKAKTVTLTGSPVMRQGNNQVSGSKVVYFIEQDRAMVES
                     DGKTRVSATIFPEELKARDQGDTKSK"
     gene            complement(2121..2795)
                     /gene="lptC"
                     /locus_tag="VMZ02_03565"
     CDS             complement(2121..2795)
                     /gene="lptC"
                     /locus_tag="VMZ02_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HUR71039.1"
                     /translation="MPDALQQLVRVLLLLCICMRKTRRILLLIVILLSLGGVAYKVAD
                     VMRQVKKVDVKSPVAILDSLPNAVLHIKDFHRAKIENGRKVWELFGDEAHYLKDKREA
                     LVRRPRFLYYDKKDQPAETSAETAHVFLNEKELERLELKGGVRVAMQGYVLTSESANY
                     LPAKEQISLPSRTQVVGEGISIEGSSMEVELEEKKIRMVRNVKTKIEPEKLAIKKSKH
                     GPKIGG"
     gene            complement(2848..3375)
                     /locus_tag="VMZ02_03570"
     CDS             complement(2848..3375)
                     /locus_tag="VMZ02_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HUR71040.1"
                     /translation="MKSFLPSVVKKAAKIRLLLLDVDGVLTDGRIIIDDRGIETKHFH
                     VRDGHGIALLKRCGIDVGFITGRRSKVVRHRAKDLRISLLVQGVQDKLLVYGRIKKKT
                     RLTDEQIAYIGDDIIDLPVLRSVGLAVTVSDGSGELKPFVHYVTLAPGGTGAVREVAE
                     LLLKAQKKWTELLPS"
     gene            complement(3386..4357)
                     /locus_tag="VMZ02_03575"
     CDS             complement(3386..4357)
                     /locus_tag="VMZ02_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HUR71041.1"
                     /translation="MSGQTIIRRAAEILRIEADGLLSVIERLDESFVQAVEIIRHCKG
                     KVVVTGVGKSGLICAKIAATLSSTGTPAFFLHSGDALHGDLGIVMPDDVVVAISNSGE
                     TDEILKLLPHFKHHSLKLIVITGNPESTLAKAGEVVLDVSVKQEACPLGLAPTTSTTV
                     TLAMGDALAVVLLEQNGFNETDFAMRHPGGMLGRKLLLRVEDLMYRGERLPLVQEMTT
                     VKEALFEITAKRLGVTGVVNDLQQLVGVLTDGDLRRGLESKGQILSLAARDLMTRNPK
                     TIPGETLASEAVAVMEQFPITSLFVLEHETKKPVGIVHLHDLIKVGL"
     gene            complement(4362..5189)
                     /gene="kdsA"
                     /locus_tag="VMZ02_03580"
     CDS             complement(4362..5189)
                     /gene="kdsA"
                     /locus_tag="VMZ02_03580"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HUR71042.1"
                     /translation="MPTKLINIGNIAVGGDRPLAIIAGPCVIESRESALRHAAALKER
                     AERVGVPYIFKSSYDKANRSSVDSFRGVGLEKGLAVLAEIKQKVGVPVLTDVHEIAQV
                     AAAKEVADILQIPAFLCRQTDFVAAVAQSGKVVNVKKGQFLAPWDMRYVIDKIVSNGN
                     QQILVTERGASFGYNNLVSDMRSLVVMREMGYPVVFDATHSLQLPGGLGQASGGERKY
                     IPALSRAGVAAGVDALFMEVHENPDQALSDGPNSLALANFEALIRKVKEIDALVKGL"
     gene            complement(5211..6857)
                     /locus_tag="VMZ02_03585"
     CDS             complement(5211..6857)
                     /locus_tag="VMZ02_03585"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HUR71043.1"
                     /translation="MATIKTKYIFVTGGVVSSLGKGLAAASIGALLESRGLKVTILKM
                     DPYINVDPGTMSPYQHGEVFVTDDGAETDMDLGHYERYVSTAMGRKNNCTTGQIYETV
                     ISKERRGDYLGGTVQVIPHITDEIKRRIFAAAEGYDIAIGEVGGTVGDIEGLPFLEAV
                     RQVAWDLGRENVLYIHLTLVPYITGGGELKTKPTQHSVKELTGLGIQPDILLCRTDRI
                     LDKKIKNKIAHFCNVAENAVITAKDVEWIYEVPLVFHEEGLDERVVEKLNIWTGAPHL
                     ERWQKVVDVLKNPKDSVRIAVVGKYMSLKESYKSLLEALVHGGIANGVRVDIDCIEAE
                     QIEQEAADILLKNVDGVLIPGGFGDRGSEGKISAIRHARENGIPFFGICLGMQMAVVE
                     FARNVCGLERANSTEFDLQTAHPVIHLMEEQKAIDTKGGTMRLGTYPCVLQNETLAAK
                     LYGREKISERHRHRYEFNNNYRAEFTARGLVLSGLSPDSTLVEIIELRGHPWFLGCQF
                     HPEFKSRPMDGHPLFRGFVEAALQNRRSVAETPRMKIVRT"
     gene            complement(6867..7640)
                     /gene="kdsB"
                     /locus_tag="VMZ02_03590"
     CDS             complement(6867..7640)
                     /gene="kdsB"
                     /locus_tag="VMZ02_03590"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HUR71044.1"
                     /translation="MSVVAIIPARFGSTRLPGKPLAQIGGRPMIQHVYQSTVKAEVLD
                     RVIVATDDRRIEAAVKKFGGEVMMTSPDHASGTDRLAEVARKIKADLLVNVQGDLPFI
                     DAKTITQAVQPLRRDRDIPMGTVCTAIYDESEWRNPNIVKVLTDKARRAMLFTRAPIP
                     FRRNDAFELEGAKTAGRHRHSVWGYRHLGLYVFRRDFLLKFSRLPPTALEQLESLEQL
                     RALENGYRIYVAQVDERSVEVDTPADLLRAENEWKRRLN"
     gene            7918..8844
                     /locus_tag="VMZ02_03595"
     CDS             7918..8844
                     /locus_tag="VMZ02_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71045.1"
                     /translation="MAELIKINAGFATVGQGAGPMMVTWKAGLFRKHGLDVARPPVMG
                     GAKGVVRGLMSGEIQFGNLAAPAPLRANLKGEADVVFLTGGINQQFLVGRPGINSREE
                     LTGKTIGTVNDGGIGDALVKFVIAKLDSVGIKGLQEVTIGHGAVAEMDALLSGKVDVV
                     VGTPPECIDAIRHGCKYLIDFAEYGLNYALGGIAGRRDYIEKNPDITRQFVKAYVEGM
                     HRYRTDREFTVDVQADYSGIADRSIAEETYDLTQPGMPRAPYPVIASLQTLLDFMARD
                     LPEARGADARNFVDDRFIRELEDNGFIKSLGI"
     gene            8899..9855
                     /locus_tag="VMZ02_03600"
     CDS             8899..9855
                     /locus_tag="VMZ02_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71046.1"
                     /translation="MPLIVHAATNDVVIDAAKREGELVYYASMNLAEANGVIGEFEKR
                     YPFIKVRLHRTGSEKLLTRVLSEFRAKKTFADIIQTVEFSMHLFNRGGVLARYIPQSN
                     GFYTKEFKEDGFWTTVYYNAYVTAYNTRLVPANQLARSYTDLLDSKWKGKLLMEGTKA
                     DWFAGMLQILGPERGVKYMRDLANQQPSPREGHELLAQLVVAGEGVYDVNIPASSVER
                     MKERGAPIDWIALGPVPAIMVGAGIATRAPHPNAAKLFLEFILSREGQKIMQTPGRHV
                     ARGDLANEQAAMLKNLKIVPVDPALAEKLDEYAKQMRSIFGG"
     gene            9892..10665
                     /locus_tag="VMZ02_03605"
     CDS             9892..10665
                     /locus_tag="VMZ02_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HUR71047.1"
                     /translation="MRFMFLNHSAQRHSAELESHIAKLLKGYASPDTTFELAYPEDLG
                     GGAVLSLLEERKALSGLHHILETPALVKKAIEAEQHGFDAVIQSNTFDPGVEASRLAV
                     RIPVIGLLRASLHFAASICDRFGLIVPLETHMPHTMRMVQTYGMTGFVSGIRTVGLYD
                     TGDLSGYHDVVIERTLAVGKELIAQGAQAIIPLGGKIYPYVVTPEELEPHLGVPVINT
                     KAVGVSYAELMARNHIKHSIKAYPCPTGLDPDAVSKRSK"
     gene            10737..11831
                     /locus_tag="VMZ02_03610"
     CDS             10737..11831
                     /locus_tag="VMZ02_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71048.1"
                     /translation="MIIDSDGHFFETEEIFDKYMEPALRNYRPRLLGDEQGHNFWVVD
                     GQTSYKRPTIKGAGAPGTAAPPGKALQSARRASPGSQTLTSLKERLDDLDREQIDLQF
                     IYPSFLLHANSWPDGVLGNGICRAYNTWLSETCNQAPDRLQAVGVVTLLDPAGAANEI
                     TRLHDMGVKAVMINGTAGARRLDHPEHDMFFAECERVRMPIAVHFSLQFPFVDKLFEH
                     HFPNRVLAGIFPIMAGLTSVLCSGLLDRYKNLKFAFLEGGISWVPAHVERMDDHFENP
                     RYGAKDLLSHPPSEYLKSGRIFFGCEGNEATLPKIVAEVGEDLFMFSSDYPHADRSEG
                     TATVLRDRRDIAAPVRQKLLETNARRFYGM"
     gene            12094..12276
                     /locus_tag="VMZ02_03615"
     CDS             12094..12276
                     /locus_tag="VMZ02_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71049.1"
                     /translation="MDFPKCQKCNNGLLIPLSDYGQDGASVMYKAWACTNPDCGFSLR
                     VDKGEVTYGKKIEHKH"
BASE COUNT         2878 a         3474 c         3154 g         2887 t
ORIGIN      
        1 cacttcgtct tgctcgaacc cgcgcgacgg tttcggttcc agtgaagcga ttaaatgcgc
       61 ggcatcaacg atgtcgtcga ccgtcacact aagatccttg gcaagttttt catagcgctt
      121 cgactcaaga tagctcaagt gaccgtcgac gacgcgagct gcgagactat cggtgagctt
      181 aagattttcg agctggacga gcaaacactc tcgcagattc cgcgccgcaa cgccgacggg
      241 atcaaagaat tggatgcgcc taagcaccgc ttcgacctct tctgcagcac attccaccgc
      301 gttgcaaatc tcctcgacgc tcagagcaag atatccgttc tcgttgatat tctggatgat
      361 atagaagccg atattcgatt cccgttcgct gatcttggac aaacgcaact gccaaatcag
      421 gtgatcttcg agtgaagttt tcttggttaa gctattttcc catgatggcg gcgagtcgcc
      481 atcatcagac atggcttcgg ccgtcagcga gctatggacg ccgttggaat ggctatcgag
      541 atactcctgc caatccaatg tcccaatctt gtccaccgta ttgggctcgc gaggagccac
      601 caattcgacg aactcttccg tcggttcgct cttagtttcg gcgcgcggct gctcggcgac
      661 ctcgggctcg tcgttcgcgc cctcttccaa agcggggttt tcttgcagct ctttgagaac
      721 catctcttcg agctcaacgc gggagagttg caataacttg atcgcctgct gcagttgcgg
      781 ggtcatcacc aacgtctgag cgagcttttg aacttgatgt aaatttagac ctgccatggt
      841 tagaacttaa aaccctctcc caaataaaat tttcgcgccc tgggacttgc ggcaattgat
      901 tgcggcgtgc cttcttctaa gatcgtgcct tcgttgacga tataagcgcg gtcgcaaata
      961 cccagcgtct ctctaacatt atgatcggtt attaagatgc cgatgccgct gatgatgaga
     1021 ttacgaacta ttttttgaat ctcggcgacg gcgatcgggt caatgccggt aaagggttcg
     1081 tcaagcagaa gaaaaaacgg cgagagcacg agcgcacggg caatttcaac acgccgtcgt
     1141 tcgccacccg ataaggtaaa tgccttgtgc gtcgcaaggt gacttactcc gagcatcttc
     1201 atgagcgcgt ctgctcgctc tttttgttca gcgctgctca aatcaagcgt ttgcaggatc
     1261 gccagcaagt tttgcgcgac cgtgagtcgg cgaaacactg aagattcctg cggcaagtag
     1321 ctgattccgg cccgtgcgcg ctgataaatg ggtgcttcag tgagattgtc ttcgttcaaa
     1381 agtacctgcc cttcatcggg ctgaaccaga ccaaccatca tatgaaatgt agtggttttt
     1441 ccggcgccgt tggggcctaa taaaccaacc acttcaccgc tctgaaccac taagttcact
     1501 ccgttcacga cacgccgtcc gccgtaagac ttggcaaccg agactgcctt gagcacactc
     1561 atttcgactt ggtatcccct tgatcccgcg ccttcaattc ctcggggaat atcgtcgcag
     1621 agacccttgt cttaccatcg ctttcaacca tggcgcgatc ttgttcgatg aagtaaacga
     1681 ccttcgagcc gctaacttga ttattgcctt ggcgcatcac ggggctaccg gtcagagtta
     1741 ccgttttggc cttatcgtca aaaatcgcgc gatcaccagt ggcaacccgg gtcccttggg
     1801 tagcgttgac cttgccgtct aaaactattt gcttcatctg cttcatttca ggatcataat
     1861 atgccgtcag tacgtcgctc cgcatggtca tatcggattg cacagcaatc acccggcctt
     1921 tgtagacaat cgtattcttc gcctgatcaa cctccatcca gtcagcagtg atatagatcg
     1981 ggtctttctt gttgaattca aaagcgtttg aagtcgtctt accctcgcct gctgccgccg
     2041 ctgctacagc atacactgac ggaaaggaaa ccgacaaaca aaggcccaag atcatggtcg
     2101 agaatgcttt ctttatcatt ctatccccca atttttgggc cgtgcttcga ttttttgatg
     2161 gccagttttt ccggttcaat cttggtcttg acgttgcgca ccatgcgaat tttcttctcc
     2221 tctaattcca cttccatact ggatccttcg attgaaatac cttcccccac cacctgagtt
     2281 cggctcggca acgagatctg ctccttggca ggaaggtaat tggctgattc cgacgtcagc
     2341 acgtatcctt gcatcgccac tcgaactccc cccttgagtt ccaatcgttc gagctccttt
     2401 tcattgagga aaacatgtgc cgtttccgcc gaagtctccg cgggttgatc ttttttgtcg
     2461 tagtatagaa accgtggccg gcgaaccagc gcttcacgtt tgtcttttaa ataatgagcc
     2521 tcatcaccga aaagttccca aaccttgcgg ccgttttcaa ttttcgcccg atgaaaatct
     2581 ttaatgtgca acaccgcatt cggcaaagaa tctaggatag cgaccggact ctttacatcg
     2641 acttttttca cttgccgcat gacatcggca actttatatg ccactccacc cagtgaaaga
     2701 agaatgacaa tcagtaaaag aattcgtctg gtcttgcgca tacaaataca gagcaaaagt
     2761 agcactctta ctaattgctg taaagcatca ggcacggaaa ttgcttcgcg tactgatgat
     2821 ttctctcgcg ttcggtgatt ttcgctttca gctaggaagc aactctgtcc actttttctg
     2881 ggctttgagt agcagttcgg caacttcccg taccgctccc gtcccgcccg gagcaagggt
     2941 gacgtagtga acaaaaggct tgagctctcc cgagccatcg ctgaccgtca ccgccaaacc
     3001 taccgagcga agtacgggca gatcaataat gtcgtcgccg atataggcaa tctgttcgtc
     3061 ggttaagcgg gtttttttct taattcgacc atacacaagt aacttgtcct gaacgccctg
     3121 aacaagtagc gagatcctca gatccttagc ccgatgacgg acaactttag accgtcgtcc
     3181 cgtgataaac ccgacgtcaa taccgcaacg ttttagcagc gcgatgccat ggccgtcgcg
     3241 gacatggaag tgcttggttt caatgccgcg gtcatcgatg ataatgcgtc catcagtcag
     3301 cacaccgtcg acgtcgagca gcagcaggcg aatcttcgcg gcctttttga caaccgaggg
     3361 taggaaggac ttcacggcgc gcttgctaaa gaccgacttt gatcaaatcg tgcaaatgaa
     3421 caatgccgac gggttttttg gtttcatgct caagcacgaa cagcgaagta atgggaaact
     3481 gctccatcac cgcaaccgct tcactcgcta aagtttcgcc cggtattgtc ttgggattgc
     3541 gtgtcatcag atctcgagcc gccaaggaaa gaatctgccc cttactctcc aatccacggc
     3601 gcagatcgcc atccgtgaga accccgacta actgttgtag atcattgacc actccggtga
     3661 cgcctaggcg cttcgcggta atctcgaaca gcgcctcttt cacagtggtc atctcctgaa
     3721 ccagcggtaa gcgctcgccg cgatacatca agtcctcgac tcgcaacaac agtttacgtc
     3781 ccaacatgcc accaggatgg cgcatggcaa agtccgtctc attgaaacca ttttgctcaa
     3841 gcagaaccac cgcaagggca tcgcccatgg ccaaagtaac cgtcgtgctg gtggtcggcg
     3901 ccaagccgag agggcaagcc tcttgtttga cggagacatc gagaacaacc tctcccgcct
     3961 tggcgagcgt cgattcggga ttcccagtga tgacgattag cttgaggctg tgatgtttga
     4021 aatgaggtag gagtttcaaa atttcatcgg tctcgccact gttggaaatc gccaccacga
     4081 catcgtctgg catcacgatg ccgagatcac catgcaaagc atcgcccgaa tgcaggaaga
     4141 acgctggggt gcccgtgctc gagagagttg cggcgatttt ggcgcagatc agaccggatt
     4201 tgccgacacc ggtaaccacc accttgcctt tgcagtgtcg tataatttcg accgcttgga
     4261 caaagctttc gtccaagcgc tcaatcacag acaagagacc gtcggcctcg atccgcaata
     4321 tctcggcggc gcgacgaatg atagtttgcc cactcacagg attaaaggcc tttgaccaga
     4381 gcgtcgattt ctttaacctt gcggatcaac gcttcaaagt ttgcgagtgc taatgaattg
     4441 gggccgtcgc tcagtgcctg gtcgggattc tcatgcacct ccatgaataa tgcatccact
     4501 cccgcggcta ccccggcgcg agagagagca ggaatatact tgcgttcgcc gccggaagcc
     4561 tggcccaaac cgcctggcaa ctgcaagcta tgcgtggcat cgaaaacaac cgggtagccc
     4621 atctcgcgca tgacgacgag agagcgcata tccgaaacta aattattata gccaaatgat
     4681 gcgccacgct cagtaactag aatctgctgg ttgccattag aaacgatttt gtcgatgaca
     4741 tagcgcatat cccagggggc gagaaactgg cctttcttga cgtttacgac cttgcccgac
     4801 tgagccactg cggcgacaaa atccgtctgg cgacagagaa acgccggaat ttgcagtatg
     4861 tcggcgacct ctttcgccgc cgccacttga gcgatttcat ggacatccgt gagcacaggg
     4921 acaccgactt tttgtttgat ctcggcaaga accgccaatc ccttttcgag acccactcca
     4981 cggaaagaat cgaccgaact gcggttggcc ttgtcgtagg aggatttaaa aatatatggc
     5041 acgccaacgc gctccgcgcg ctctttgagc gcggcggcat gacgcagcgc cgactcccgg
     5101 ctctcgatta cacaaggccc ggcgatgatt gccagcgggc ggtcaccgcc tacggcaatg
     5161 ttaccgatgt ttatcagctt agtcggcatt tgagtcgcag cattttacaa tcacgtgcgg
     5221 acgatcttca tgcgcggcgt ctctgccact gaacgccggt tttgcagcgc cgcctcgaca
     5281 aagccgcgga acaacggatg gccgtccatc gggcgcgatt taaactccgg gtgaaattga
     5341 cagccaagaa accaggggtg gccacgcagc tcaatgatct ccaccagtgt gctatccggt
     5401 gacaggccgc tcaataccaa ccctctggca gtgaattcgg cccggtagtt gttgttgaac
     5461 tcgtaacgat ggcggtgccg ttcggaaatc ttttcacgac cgtacaattt ggcggcaagc
     5521 gtctcgtttt gcaggacaca aggataggtg cccaagcgca tggtaccacc tttggtgtcg
     5581 atcgccttct gctcttccat caagtgaatt actggatgcg ccgtttgtag gtcaaactcg
     5641 gtcgagttag cacgttccaa gccgcacacg ttgcgggcaa attccactac ggccatctgc
     5701 atgccaagac agataccgaa aaacggaata ccattttcac gggcatgacg aattgcagaa
     5761 atttttccct cgctgccacg atcgccgaaa ccacccggga taagcacgcc gtcgacattt
     5821 ttgagcaaaa tatccgccgc ttcctgctca atctgctccg cttcgatgca atcgatgtct
     5881 actctaacgc cattggcgat gccgccatgc accagcgctt ccaaaagact tttgtaagac
     5941 tccttaagac tcatgtattt gccgaccacc gcgatgcgaa ctgagtcttt gggattcttc
     6001 agaacatcga cgactttctg ccagcgctcg aggtgcggcg cgccggtcca gatgttgagc
     6061 ttttcgacaa ctcgctcatc gagcccttcc tcgtggaaga ccagaggtac ttcatagatc
     6121 cactcgacgt ccttggctgt gatcaccgcg ttttcggcga cgttacaaaa atgagcgatc
     6181 ttatttttga tcttcttatc gagaatgcga tccgtgcggc agagcaaaat atcgggctga
     6241 atgcctaagc cggtcaattc cttaacgcta tgctgtgtcg gcttggtttt aagctcacca
     6301 ccgccggtga tgtatggcac cagagtcagg tgaatgtaga gaacattctc gcggcccaaa
     6361 tcccacgcca cttggcgcac cgcttccaaa aacggcaaac cttcgatgtc gcccaccgta
     6421 ccgccaactt cgccaatggc aatgtcatag ccttcggcgg ctgcgaagat gcgccgtttg
     6481 atctcatcag tgatatgcgg gatcacttgc accgtaccgc caaggtaatc accgcggcgt
     6541 tccttggaaa taaccgtctc gtaaatctgc cctgtcgtgc aattgttttt ccgtcccatc
     6601 gcggtggaga catagcgttc gtagtgaccg agatccatat ccgtctcggc accatcatca
     6661 gtaacaaaca cctcgccatg ctggtatgga ctcatggtgc ctgggtcgac attgatgtac
     6721 ggatccattt taaggatcgt cactttaagc ccgcggctct ccaaaagcgc tccgatagaa
     6781 gccgccgcca gccccttgcc caaagatgaa acgacaccgc ccgtgacgaa aatatattta
     6841 gtcttgatcg tcgccatcgc cacctctcag tttaaccgcc gcttccattc gttttccgcg
     6901 cgcaggagat cggctggagt atccacctcc acagagcgtt cgtctacctg cgctacataa
     6961 attcggtaac cgttttccaa ggcgcgcaac tgttccaaac tttcgagctg ttccaacgcg
     7021 gttggcggaa ggcgcgaaaa tttcagtaag aaatcccggc ggaaaacgta cagaccgaga
     7081 tgacgatagc cccaaacaga gtgccgatgt cgtcctgcgg tcttcgcccc ttcaagttcg
     7141 aaggcatcgt tgcgccggaa cgggatcggc gcacgggtaa aaagcatcgc ccgcctcgcc
     7201 ttatcggtga gcactttaac gatatttgga ttgcgccatt cgctttcatc gtatatcgcc
     7261 gtgcacacgg tacccatggg aatgtcccgg tcgcgtcgca acggctgcac tgcctgagta
     7321 atggtcttgg cgtcgatgaa cggcaaatcg ccctgcacgt ttaccagcag gtcggccttg
     7381 atcttgcgcg cgacctcggc taagcgatcg gttccactgg cgtgatcggg ggatgtcatc
     7441 atgacttctc cgccgaattt tttcaccgcc gcctcaatgc gccgatcgtc ggtcgccaca
     7501 atcactcggt ctaacacttc ggctttcacc gtgctctggt aaacatgttg gatcatcggc
     7561 cggccgccga tctgagctag cggtttaccc ggtaaacggg tcgatccaaa gcgcgccggg
     7621 ataatcgcca caacgctcat ggattcacga tttcatggac ctaatgacgt ctgaatgaat
     7681 ggttaataat ggcatgagca tcgccaaaaa taaaaaaccg caatggcttt cctggaaagc
     7741 gcatcacgga attcgataat acgcccgcgt ccaaaagctg tcaacgcgat tcgacccatt
     7801 cccacctgcc gagagcaaaa tagcttccca ggtgacaact gttattaatc gcgcttgact
     7861 gatcgccgca cagtcgtgat aggaatggaa agagatctgg agaaatggaa ataacgcatg
     7921 gcagagttaa tcaaaatcaa tgccggcttc gccacggtag gccagggcgc ggggccgatg
     7981 atggtaacgt ggaaagccgg cttgttccgc aaacacggac tggatgtcgc tcggccgccg
     8041 gtgatgggcg gcgccaaggg tgtcgtgcgc ggcttgatgt cgggtgaaat tcagtttggt
     8101 aacctggccg cgccagcgcc gctgcgcgcc aatcttaaag gcgaagctga tgtggttttt
     8161 ttaacaggcg gcatcaacca gcaatttttg gtcggacgtc ccggcatcaa cagccgcgag
     8221 gaactgaccg gcaagacaat tggcacagtc aacgacggcg gcatcggcga cgcactcgtt
     8281 aagtttgtta tcgcgaaact cgacagcgtc ggtatcaagg gactgcagga agtgaccatc
     8341 ggtcatggcg ctgttgcaga aatggacgct ctactcagcg gcaaagtcga tgtcgtcgtg
     8401 ggcacgccgc ccgaatgtat cgacgcgatc cgccatggct gcaagtatct aatcgatttc
     8461 gcggaatacg gcctcaacta cgcattgggt ggcatcgccg ggcggcgcga ttacatcgag
     8521 aaaaatcccg atatcactcg gcaatttgtc aaagcctatg tcgagggcat gcaccgttat
     8581 cgcaccgacc gagagtttac cgtcgacgtt caggccgatt acagcggcat cgccgaccgt
     8641 agcatcgccg aagaaaccta cgacctcact cagcccggca tgccgcgggc gccctacccc
     8701 gtcattgctt cactacaaac attactcgac tttatggcca gagatttacc cgaggccaga
     8761 ggcgcggatg caaggaactt tgtcgacgat cgatttatcc gcgagctgga ggacaacggt
     8821 tttatcaagt cattaggtat ttagatgaag tcgggcaagt tcagagttgc gctaatcgct
     8881 ctactgtggc actgacttgt gccgctgatc gtccatgcag ccactaacga tgtcgtgatc
     8941 gatgccgcca agcgcgaggg cgagttggtc tattacgcat cgatgaatct cgccgaagcc
     9001 aatggcgtaa tcggcgaatt cgaaaagcgc tatccattta tcaaagttag gctgcaccgc
     9061 accggcagtg agaagctgct aacccgggtt ctcagtgaat ttcgcgccaa aaaaactttt
     9121 gccgacatta ttcagaccgt cgagttcagc atgcatttgt tcaaccgcgg cggcgtgctg
     9181 gcgcgctaca tcccgcagtc aaatggattt tacaccaaag aatttaagga agacgggttt
     9241 tggacgacag tttactacaa cgcctacgtt accgcttaca acacgcgttt ggtgccggct
     9301 aatcagctgg cgcgatctta taccgacctg ctcgatagta aatggaaagg taaactgctg
     9361 atggagggga ccaaggccga ttggttcgcc ggcatgttgc aaatcctcgg accagagcga
     9421 ggtgttaaat acatgcgcga cctagccaac caacaaccca gcccccgtga aggccatgaa
     9481 ctgttggctc aactggtcgt cgccggcgaa ggcgtttacg atgtcaatat tccagcgtca
     9541 tcagtcgagc gcatgaaaga acggggtgcg cccatcgatt ggatcgcgct tggcccggtg
     9601 ccggcgatta tggtcggcgc cggcatcgcc acacgcgccc ctcatcccaa cgccgctaaa
     9661 ttgtttttgg aatttatttt atcgcgcgag ggacagaaga tcatgcagac tccagggcgc
     9721 cacgtcgccc gcggcgacct tgccaacgaa caggcggcaa tgctcaaaaa tttgaagata
     9781 gtgccggtcg atcctgcatt ggcagaaaaa ctcgacgagt acgccaagca aatgcgcagt
     9841 atcttcggcg gatagttggg catccatctc tgatcagcga aggagaaaat catgcggttt
     9901 atgttcttaa accattcggc tcagcggcac tccgctgaac tcgaatcaca cattgccaaa
     9961 ctgctcaaag gctacgcttc gccggatacc acctttgagc ttgcctatcc cgaagacctt
    10021 ggcggcggcg ccgtcttgag tctgctcgaa gagcgcaaag cgctatctgg cctgcatcat
    10081 attttggaaa ccccggcgtt ggttaaaaaa gccatcgaag ccgagcagca cggatttgac
    10141 gcggtgatcc agagcaatac tttcgatccg ggtgtggaag cgagccgttt ggcggttcgg
    10201 atcccggtaa tcggcttgtt gcgcgcgtcc ttgcacttcg ccgcgagcat ctgcgaccgc
    10261 ttcgggctca tcgtgccgtt ggaaacccat atgccgcaca ccatgcgcat ggtgcaaacc
    10321 tacggcatga ccggattcgt cagcggcatc cgcaccgtcg gtctttacga cacgggcgat
    10381 ctctcgggct atcatgatgt tgtcatcgag cgtacgcttg cggtcggtaa agaattaatc
    10441 gctcaaggcg ctcaagcgat aattcccctg ggcggcaaga tttatcccta tgtggtcacc
    10501 cccgaggagt tggaacccca tctcggcgtg ccggtaatca acaccaaggc cgtgggcgtg
    10561 agctatgcgg aactcatggc gcgcaaccat atcaaacaca gcatcaaagc ttacccctgc
    10621 ccaacggggc tcgatcccga cgcagtttcg aaacgctcga agtagcgaca gtagattgcg
    10681 acgggttcgt gcaagtgcag ggagttgatt tgactctcga aggagaagcc cactcaatga
    10741 ttatcgatag cgacggacat ttttttgaaa ccgaagagat cttcgacaaa tacatggagc
    10801 ccgccttgcg caactatcgg ccgcgcctat tgggcgacga acagggccat aacttttggg
    10861 tggttgacgg ccagacctct tataaacggc cgaccatcaa aggcgccggc gcgccgggta
    10921 cggcggcccc tccgggaaaa gcattgcagt ccgctcggcg cgcttcaccc gggagccaaa
    10981 ccttgaccag tttgaaggag cgattggacg atctcgaccg cgaacaaata gatttacagt
    11041 ttatctatcc aagctttttg ctgcacgcga attcctggcc cgatggtgtg ttggggaacg
    11101 gtatttgtcg cgcgtacaac acctggctta gcgaaacctg taaccaagca cctgatcggc
    11161 tacaagccgt cggtgtcgta actttgctcg atcccgcagg cgccgccaat gaaatcactc
    11221 ggctccatga tatgggagtg aaagcagtga tgatcaacgg caccgcgggt gccaggcgct
    11281 tggatcatcc cgaacatgat atgttctttg ccgagtgcga gcgagtgaga atgcccattg
    11341 cggtacactt cagtttgcag ttcccgttcg tcgacaaatt gttcgagcac cactttccca
    11401 accgtgtgct tgccggaatt tttccgataa tggcaggtct caccagcgtg ctctgctccg
    11461 gccttttgga tcgctacaaa aatctcaagt tcgcatttct cgaaggcggc atcagttggg
    11521 tgccagccca tgtcgaacgc atggacgacc actttgaaaa tccgcgctat ggcgcgaagg
    11581 atctcctcag tcatccgccc agtgagtatc tcaagagcgg ccggatattc tttggctgcg
    11641 aaggcaacga agcaacgctg cctaaaatcg tcgcagaggt cggcgaggac ctgtttatgt
    11701 tttcttcgga ttatccccac gccgaccgca gcgaagggac ggcgacggtg ctcagagatc
    11761 gtcgcgatat tgccgcgccg gtgcgtcaga aactactaga gacaaatgct cgccgctttt
    11821 acggtatgta atcgattatc atcaaaactt agctgccgga aattttcaag gatgtaactg
    11881 ccgaattcgc agattagcgt gtccggcata atgaaacggg taatcggaac gtcgattgcc
    11941 ccatttttct ggttctcagg tcattcgaac ggcagtcgtg atttgaccgc taaaattcgg
    12001 cgcgcttgat ttaccctctt ccgatactat aaaatttttc tcgttggaac gagatcatgc
    12061 ccccctgtcg aggtgatgca aggaggagtg gtgatggatt ttccgaaatg tcagaaatgc
    12121 aataatggcc tgctcatccc gctttcggat tacggccaag atggcgcctc ggtgatgtac
    12181 aaagcgtggg cgtgtaccaa ccccgactgt ggcttttctc tacgtgtcga caaaggtgaa
    12241 gtcacctacg gcaagaagat cgaacacaaa cattgatagt cgatcgctga gtcaggtaca
    12301 catttctagc cggcatggtt cgattgaagc tctgaatcgc ccgcccaaaa agctcgcaac
    12361 ctctcttcaa agccgccaaa catcgctgct gca
//
LOCUS       DATLCH010000123         4382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9321777, whole genome shotgun sequence.
ACCESSION   DATLCH010000123 DATLCH010000000
VERSION     DATLCH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9321777"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..511
                     /locus_tag="VMZ02_03620"
     CDS             242..511
                     /locus_tag="VMZ02_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71050.1"
                     /translation="MAVFPQSKNDDSKMMLGFIVRRCAADIGHTPSPDEFAKWANGDN
                     LKGRRFCLFGREISPTAAKLMFGQPGRLVTVSPEGMARISKANGR"
     gene            1003..2004
                     /locus_tag="VMZ02_03625"
     CDS             1003..2004
                     /locus_tag="VMZ02_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71051.1"
                     /translation="MLVRGLTVLFALVLASSAVAPVSAQVKSEKLRIGYAARAVAHSV
                     PFVAREAGFFAEEGINAEIIHTAGSIAPMALAANEVDMTIMSAYLMIPVAAQNRDIVM
                     LGGFSRYASMVFVARPDIKTPQDLKGKIVGIQRPGDAYEKSARFALKHIGLDADKDVK
                     LLALGSNDVMWGALSTNRVAATVLSPPGTLFARKAGMNFMVDLTDLKLEYQGSTITTR
                     RSLVQKNPQLATRAMRAIVRGVHFLKTRKDETTRILSKFLGTKDPEGLTESWEYAAKM
                     PAKPYAVESAVQAVLDHLSEGQPKYAQYKPGEFIDARTLTEIDKSGYIDKLYAGQAR"
     gene            2018..4060
                     /gene="uvrB"
                     /locus_tag="VMZ02_03630"
     CDS             2018..4060
                     /gene="uvrB"
                     /locus_tag="VMZ02_03630"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HUR71052.1"
                     /translation="MLRDEDSGQFRLVCEFKPQGDQPQAIDKLVKGLAAKRQHQVLLG
                     VTGSGKTFTMANVIAQVNRPTLVIAPNKTLAAQLYEEFRTLFPENAVRYFVSYYDYYQ
                     PEAYLPSSDTYIEKDSSINDEIDKLRHSATKALLERRDTLIVASVSCIYGLGSPEAYF
                     DLMVYVEEGTSVNRDQMLRKLVDIQYQRSDYDFHRGTFRVRGDMVEVFPAYEDRSALR
                     MEFFDDIIESIHEIDPIRGKVIRRVDKATIYPASHYVTGQDRRKQAVLGIRAELKERL
                     KELQDQRKLLEAQRLQQRTLYDLELLEEMGFCPGIENYSRHLTGRQPGEPPPTLLSYF
                     PNDFLLVIDESHVTVPQIGGMYRGDRSRKETLVEYGFRLPSALDNRPLNFDEFAAMIN
                     QTIYISATPAPFELRESNGAVVEQVIRPTGLMDPAIIVRPARTQVDDLLEEIRKRVER
                     KERVLVTTLTKRMAEDLTEYYQDLNVKVRYLHSDIETLERVEIIRQLRSGQFDVLVGI
                     NLLREGLDLPEVSLVAILDADKEGFLRSDRSLIQTIGRAARNVNGTVILYADGITDSM
                     KRAMDETERRRRLQGAFNKKHGVTPQTIIKALSSPLVKIYDADYFDIPIAAEGALKYR
                     PGELNRKLRKLQKEMKEAAQKLEFEHAAELRDQIRQLQSEELTLRDSPIDANFDQQ"
     gene            complement(4122..>4382)
                     /locus_tag="VMZ02_03635"
     CDS             complement(4122..>4382)
                     /locus_tag="VMZ02_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71053.1"
                     /translation="GFMVQWGEYLSSTGSMQPFVLYSLSGLAIAVFFVATIFNDPPKT
                     SARFVHYRPTPGDNTDERFTAGRRDPVDLAVNVSQISENENR"
BASE COUNT         1016 a         1154 c         1243 g          969 t
ORIGIN      
        1 ggcgatcgct gccaagcaac aaaaagtatt tcgagcacga ctaacgtaga agctgttcag
       61 gtagaagggt gaaccggcca cgccgccggc tcgctttttt ttggcttgtc gacgccggtc
      121 acatagcaaa tttcggggta agaaggcact gatattcgac aggatagttc ttataaaaat
      181 tcggaaattt ctcgcgagaa tttcttgcaa cacgaataaa ctttttctat acatttgcgt
      241 gatggctgtc ttccctcaga gcaagaacga tgattcaaag atgatgctcg ggtttatcgt
      301 gcgtcgttgt gccgcggaca tcggccacac gccgtcgccc gatgaatttg ccaaatgggc
      361 caatggcgat aatctgaagg gaaggcggtt ttgtttgttc ggtagggaga tctcgccgac
      421 cgcggcaaaa ttgatgtttg gtcaacccgg tagactcgtc accgtgagcc ccgaaggcat
      481 ggcgcggatc tccaaggcca acggccggta aaaacctttg ccggttgccg ctttgctctc
      541 gatagtttcc ttcttttctc gattcaatgt gcggttctag cggctatgca gataaatagg
      601 ccgtctgcta gcgacactat ctacagcttg tcatttgaga gctacctatc gtcaaaacaa
      661 gtcgaagcag cgcgacacca acaaacgcgt caacaccagc ccagtcgtcc aaagcacgct
      721 gatcaagatg ccgctgcgcg gctttaggcc aaattgccgt tggcttattg agcggcttgc
      781 cgtgcgtcgg caagcaccgt ggcgggatca gcttgagcgc caggtagatg cccaggggca
      841 acagcacgga tcgcccgcgc tttttgccgt aaggatttcg atcagcctct gcacatcgat
      901 gacggcgcat tcagatggtg attgccccgt cccaagcgtc ttgttatgct tttccatcct
      961 atctgtattt tgctttctgg atctcgcgga ggatgccatt tgatgcttgt tcgtggatta
     1021 acagttttat tcgctttggt tttagcctcc agtgctgttg cgccggtttc ggcccaagtt
     1081 aagtcagaaa aactacgtat cggctatgcc gcccgcgctg tcgcccactc ggtgcctttt
     1141 gtcgccagag aagcaggatt ctttgctgaa gaaggtatca atgctgagat catccatacg
     1201 gccggatcca tagcacccat ggcactggcg gccaacgaag tcgatatgac gatcatgtcg
     1261 gcctatttaa tgattccagt ggcggcgcag aatcgcgata tcgtcatgct cggcggtttc
     1321 tcccgctatg cgtcgatggt ttttgtggcg cgaccggata tcaaaactcc ccaggattta
     1381 aagggcaaaa tcgtcggcat tcagcggccc ggggacgcct atgaaaagag cgctcgcttc
     1441 gccttgaaac atatcggtct tgatgccgac aaggatgtca aactgcttgc cctcggcagc
     1501 aatgacgtaa tgtggggcgc tctcagtacc aaccgggttg cggccacggt gctgtcgccg
     1561 ccgggaactt tgtttgctcg caaagcaggc atgaacttca tggtcgatct gactgatctg
     1621 aagttggaat atcaaggctc gacaatcacc acgcggcgct cgctggtgca gaaaaatccc
     1681 caactcgcga cccgagcgat gcgggcgatc gtccgcggtg tgcattttct caaaacgcgc
     1741 aaagacgaga ctacgcggat cctcagcaag tttctgggca ccaaagatcc cgaaggtttg
     1801 acggaatctt gggaatacgc ggcgaaaatg ccggccaagc cctacgctgt cgagagcgcg
     1861 gtccaggcag tgctcgatca tttatccgag gggcagccga agtacgcgca gtacaagccc
     1921 ggcgaattca ttgacgcgcg cacgcttacg gaaatcgaca agagtggcta tatcgacaag
     1981 ctttatgctg gacaggcgag atagctcgga gcacgccatg cttcgcgatg aagacagcgg
     2041 acagttccgg ttggtttgcg agtttaaacc gcaaggcgat cagccccagg ccatcgacaa
     2101 actggttaag ggacttgcgg ccaagcgaca gcatcaggtg ctgttgggcg tgaccgggtc
     2161 gggcaagacc ttcacgatgg cgaacgtcat tgcccaagta aaccggccaa ccctcgtcat
     2221 agcgccgaac aagacgctgg cggcgcaact ctacgaagag tttcgcacac tgtttcctga
     2281 aaacgccgtg cgttatttcg tcagctacta tgattactat cagccggaag cgtatctacc
     2341 gtccagcgac acgtatatcg aaaaagattc gtcgatcaac gacgaaatcg acaagctgcg
     2401 ccactcggcg accaaggcgc tgctcgaacg gcgcgatact ctaatcgtcg ccagcgtctc
     2461 gtgcatctac ggtttgggct cgccggaagc gtatttcgat ctgatggtct atgtcgaaga
     2521 agggacgtcg gtcaatcgcg atcagatgct gcgcaagctc gtcgatatcc aatatcaacg
     2581 gagcgattac gattttcatc gaggcacgtt tcgcgtgcgc ggtgacatgg tcgaagtctt
     2641 ccctgcttat gaagaccgct cggcgctgcg catggaattt ttcgacgata tcattgaatc
     2701 gatccatgag atcgacccga ttcgtggcaa ggttatccgc cgcgtcgaca aagcgacgat
     2761 ttatccggcc agccattatg tcacggggca agaccgccgc aagcaggcgg tgttggggat
     2821 tcgtgcggag ctgaaggagc gcctgaaaga gctgcaggat cagcgcaagc tattagaggc
     2881 gcaacgttta cagcagcgta cgctttatga tctcgaactg ttggaagaga tgggtttttg
     2941 ccccgggatt gaaaactatt cgcgccactt gaccggccgc cagcccggtg aaccgccgcc
     3001 gactttgctg agttactttc ccaatgattt tctcctggtg atcgatgaga gtcatgtaac
     3061 tgtaccgcag atcggtggca tgtaccgcgg tgaccgctcg cgcaaagaaa ccctggtcga
     3121 gtacgggttc cgcttgccgt cggcactcga caatcggccg ctcaattttg atgaattcgc
     3181 ggcgatgatc aatcaaacga tctacatttc cgcgacgccg gcgcctttcg agttgcgcga
     3241 aagcaacggc gcggtggtcg agcaggtgat tcgccccacc ggtttgatgg acccggcgat
     3301 catcgtgcgt ccggcgcgaa cgcaagtgga cgatttgttg gaggagatcc gcaagcgcgt
     3361 cgagcgcaaa gagcgcgtgc tggtgacgac cttgaccaag cgcatggcgg aggatctgac
     3421 ggaatattat caagatctta acgttaaagt gcgctatctg cattccgata tcgagacgct
     3481 tgaacgggtg gagattattc gccaactgcg cagcgggcag ttcgacgttt tagttggcat
     3541 caatcttttg cgcgaaggtt tggacctgcc cgaagtctcc ttggtggcga ttctcgacgc
     3601 cgacaaggaa ggttttctac gttccgaccg gtcactgatc cagaccatcg gacgcgccgc
     3661 gcgaaacgtt aacggcacag tgatacttta tgccgacggc attaccgatt cgatgaagcg
     3721 cgccatggat gaaaccgagc ggcgccgccg cttgcagggc gcgttcaaca aaaaacacgg
     3781 agtaacgccg caaaccatca taaaggcgtt gagttcgccg cttgtgaaga tctacgacgc
     3841 ggactacttc gacattccca tcgcggcgga aggggcgttg aagtatcgcc ccggtgagct
     3901 taatcgaaag ttgcgaaagt tgcagaagga aatgaaagaa gcggcgcaga aactggagtt
     3961 cgaacacgcc gcggaattgc gcgaccagat tcgccagctg caaagcgagg agttaacgct
     4021 gcgggattct ccaatagacg ctaattttga tcaacaatga agcattggcg agagctcagt
     4081 caactgagga aattgacgcg cagcaatcgg ccagattttt tctagcggtt ttcgttctcc
     4141 gaaatttggc taacgttgac cgcaagatcg accgggtcgc gccggcccgc agtgaatcgc
     4201 tcgtctgtat tgtcgccggg tgttgggcgg taatggacaa agcgcgccga ggtcttcggt
     4261 gggtcgttga aaatcgtcgc gacgaagaag acggcgatgg caaggccgct gagcgagtag
     4321 agaacaaacg gctgcatcga acccgtggaa gaaagatact cgccccactg aaccatgaag
     4381 cc
//
LOCUS       DATLCH010000124         5869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9322230, whole genome shotgun sequence.
ACCESSION   DATLCH010000124 DATLCH010000000
VERSION     DATLCH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9322230"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VMZ02_03640"
     CDS             <1..898
                     /locus_tag="VMZ02_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71054.1"
                     /translation="VQAQDPRKVRLAYSAFSISFLSLFVARDAGLFKKHGIEPELVQM
                     AGPLPVAALASGEIDYLTGYTTGIIATGQGAPLKGILIALRKPPFYLVAEPNIQRIND
                     LSGKRIGVDRIGSLQHLVVRLLMRTKGADPEKAIFTQTGSVSNTVGSLGQGAVSAAVL
                     SGPHNVIMTQKGFRQLGAADELPMQFPTSGLVVNESRLKSDAARIKNVIRVMLEAAAF
                     CRKERGWVINYIRDKWAVEQKTAETVYDQWLGILTPDGKIAIKDMQEYFDLAYAQKQI
                     AAPINVGAVTDYTLIDQVLAGK"
     gene            984..1655
                     /locus_tag="VMZ02_03645"
     CDS             984..1655
                     /locus_tag="VMZ02_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR71055.1"
                     /translation="MVDGKALVASLREETIEPGIRSLMNTQYFTELRQGKLSVKRMQG
                     WAIQHYLHNKAILKSFALGMVKNAHEPIMFDYFTEQFTEEQSHPDLAKRFGLALGLTE
                     EDFTEATQMFECQIHSARTVYNTLMGYAPQNRASALTSESMVCRYSEEFLNAGRKHYG
                     LSDRDLTFFSVHMVADQEHTARSAELIARMVKTPREERLVRESAQYMVRIKLGKFDGL
                     YREYA"
     gene            1667..2671
                     /locus_tag="VMZ02_03650"
     CDS             1667..2671
                     /locus_tag="VMZ02_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71056.1"
                     /translation="MNIFALLLFVLLAESYAWAQSDLNSLSAGAKNDKEVVWYTTTSA
                     GDNQAIVAGFNKKYPFLKVQVLRSTGEKLRQRVLAETQGGQFFSDVLSVSSMEMGLLK
                     SRNLLQPYDPPDAESYPANARDKEKAFTAIYARNFVMGYNTAMLSEKERPRDWSDLLA
                     PFWKGKFAMDEEEFEWFGTLVDYWGREKAGKFIRALAAQQPQLRRGHSLLAQLLAAGE
                     FHAAIVFPFEIEQLKLKGARVDWAATSDPIVTSINVMALSAKAPHVNAGKLLINYVLS
                     EEGQTIIKSLSRVPIRPGVKPTIAKLDQSALKIHYVPADMFRKVAEYEKEFRELFWKK
                     "
     gene            complement(2885..3568)
                     /locus_tag="VMZ02_03655"
     CDS             complement(2885..3568)
                     /locus_tag="VMZ02_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HUR71057.1"
                     /translation="METKIFTWLGREFLEITGEATAGSSVESATAELFERFDTALKSN
                     GLSLDNTARIRVYGRDRQARTDATVARAKILSGKRRAASSSFISKQWFDSDGSGGLEL
                     LAMRPLNATVQRNPIDFEPARTYLCYLEYDALLFFSGFTSEAATLAQQVKDVLGTLDN
                     AFARAKSDWSNVEKLSLLLQRGSNVQEVRQVLAAADRLTVPEVEFTFVDGFAGEKYLV
                     EIEATAVLS"
     gene            3780..4736
                     /locus_tag="VMZ02_03660"
     CDS             3780..4736
                     /locus_tag="VMZ02_03660"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71058.1"
                     /translation="MLASRLTNTTFALLVSLLFVTAAFAQPTKIRLASTAATSTEKIA
                     FALAKDAGILKKHNIDLELILITGGPVAMQALLAKSLDIITTSATVFLHGFVEGADVK
                     IVGGVNNRFPYTFFTRPNITAPAQLKGKTLGITRYGSTDELATRMALEQFGLNPKSDV
                     KFIQGGGSAGRINALQAGSIDGTSLISGVSHVAKKAGMNPLLDFAEKEIDYQMTGVVA
                     RGDFLKSSPEGLRRFLRAYVESIRFYKANRSEAIRETMKAIRTDDRQLAEADYNYRAR
                     AFPDDGKPTLKGIQLAIDELVKENPKAKTVSPQQVIDLSFLP"
     gene            4741..5142
                     /locus_tag="VMZ02_03665"
     CDS             4741..5142
                     /locus_tag="VMZ02_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71059.1"
                     /translation="MFRRLFTAEGQSRSARTVEIFGWLILAEGTSVVLLPQLAVGLLH
                     LPPLVGQAENYFRLVGILVSGVGMLYVVSGRLNAEGFVFASMLDRPLVPVAMAILWHL
                     EIIPGTLALIFSLQDFGSFVWTLVTWRSEHR"
     gene            5168..>5869
                     /locus_tag="VMZ02_03670"
     CDS             5168..>5869
                     /locus_tag="VMZ02_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUR71060.1"
                     /translation="MSNPIIGFIGLGIMGKPMARHLIKAGYPLVIHNRSRAAIDELSK
                     EGAQMAANSQEVAQRSEVIITMLPDSPDVELVYAAEHGIFAGVKSGTLLIDMSSISPV
                     VARKLAAEAEKRGCDMLDAPVSGGEAGAIAASLSIMIGGEAGAVARAMPIFEKLGKNI
                     VHVGAAGAGQVTKAANQMVVGTTIAIVSEALVLAAKAGVDPAKVRQALLGGFAQSKIL
                     EAHGQKMLDRNFKPGF"
BASE COUNT         1355 a         1653 c         1597 g         1264 t
ORIGIN      
        1 cgtgcaggcc caggatccgc gcaaggttcg tttagcctat tcggcattta gcatttcctt
       61 tctcagtctc ttcgtcgcgc gcgatgccgg actctttaaa aaacatggta tcgaaccaga
      121 gctcgttcag atggccggac cgctgccggt tgccgctttg gcctcggggg agattgatta
      181 tctcacagga tacaccaccg gcattatcgc gaccggtcaa ggcgcgccgc tcaaaggtat
      241 cctcattgca ttgcgcaaac cgcccttcta cctcgtcgcc gagccgaata ttcaacgcat
      301 caatgatctt tcgggaaagc gcattggcgt cgatcgcata ggcagtttgc agcacctggt
      361 ggttcgtttg ctcatgcgta ccaaaggtgc cgatcctgaa aaagcgatct ttactcagac
      421 cggctcggtg tccaacacgg tcggcagctt gggccagggc gcggtgtctg ctgcagtcct
      481 gtcgggcccg cacaacgtta tcatgacgca aaaaggcttt cgccaactcg gcgccgcgga
      541 cgaattgccg atgcagtttc ccactagcgg cctagtcgtt aacgagagca gattgaagag
      601 cgatgccgcg cgaataaaaa atgttatccg tgtcatgctc gaagctgcgg cgttttgccg
      661 caaagagcgc ggctgggtga tcaattacat tcgcgataag tgggcggtcg aacaaaaaac
      721 cgcggagaca gtctacgacc agtggctggg aatcctcaca ccagacggca aaatcgccat
      781 caaggacatg caggagtatt tcgatctggc ctatgctcaa aaacagattg ccgcgccgat
      841 caatgtcggc gcagtcactg actacacctt gattgatcaa gtgttggcag gaaaatagtt
      901 gtcagcggcc cataatcccg tcgagttgaa ttgatactgt aaactataga gtctagacgg
      961 caaataaaaa taaggagttt tccatggtag acggcaaagc actcgtcgct tcgctccgcg
     1021 aagaaacaat cgagccgggg atccgaagtt tgatgaatac gcaatatttt accgagctgc
     1081 ggcaaggcaa gttatcggtc aagcgaatgc aaggctgggc gatccagcac tatctgcaca
     1141 acaaggcgat cctaaaatca tttgccctcg gcatggtgaa gaatgcccat gagcccatca
     1201 tgttcgacta ttttaccgag cagtttaccg aagagcagag ccatccggat ttagccaagc
     1261 ggtttggtct tgccctcggt ttgaccgaag aagactttac cgaggcgacc cagatgttcg
     1321 agtgtcagat tcattccgcg cgcacggtct acaataccct gatgggatac gcaccgcaaa
     1381 atcgcgcctc ggcgctaacc agcgaaagta tggtttgtcg ttactcggag gagtttttga
     1441 acgctggacg taagcactat ggcctttccg atcgcgattt gaccttcttc agtgtccata
     1501 tggtcgccga ccaagagcac actgctcgct ctgcggagct gattgcacgt atggtcaaaa
     1561 ccccgcgcga ggagcggctg gtacgtgaaa gcgcgcaata catggtacgc atcaagctcg
     1621 gcaaattcga cgggctctat cgcgaatacg cctgagaggc atgggtatga atatctttgc
     1681 gctactgctc ttcgtcctat tggcggaatc ctatgcctgg gcgcaatcgg atctgaactc
     1741 gctcagcgcg ggcgcgaaga acgacaagga agtcgtctgg tacacaacga cttctgccgg
     1801 cgacaaccaa gcgatcgtcg ccggctttaa caagaagtat ccgtttttga aagtccaagt
     1861 tctgcgcagc accggcgaga aactgcgcca gcgcgtcttg gcggaaactc agggaggaca
     1921 atttttctcc gatgtcttga gcgtcagcag catggaaatg ggtttgctca agtcgcgcaa
     1981 tctgctgcaa ccctacgacc caccggacgc ggaatcatat cccgccaacg ccagggacaa
     2041 agaaaaggct ttcacggcga tctacgcgcg caactttgtc atgggctaca acaccgccat
     2101 gctcagcgaa aaagagcggc cgcgagattg gtccgacctc ctggcacctt tctggaaggg
     2161 caagttcgcc atggacgaag aagagttcga gtggttcggc acattggtcg actattgggg
     2221 acgggaaaag gccggcaagt tcatccgcgc actcgcggcg cagcaaccgc aactgcgccg
     2281 tggccatagc ctgctcgccc aacttcttgc cgccggcgag tttcatgccg cgattgtttt
     2341 tccattcgaa atcgagcagc taaaactaaa aggcgcgcgc gtcgactggg ccgcgacttc
     2401 cgatcctatt gtcacgtcga tcaacgtcat ggcgctgtcc gccaaagcac cccatgtaaa
     2461 tgccggcaag ctgctgatca actatgtctt gtcggaagaa ggccagacaa ttatcaagag
     2521 tctctcccgc gtgccgatcc gtcccggcgt caagccgaca attgccaagc tcgatcaatc
     2581 ggcgctgaaa attcattacg tcccagccga catgtttcgc aaagtcgccg agtatgaaaa
     2641 ggaattcagg gagctattct ggaaaaagta ataagcgttg gtacgttgtt tctaaggatt
     2701 acggaattat cctcggcttt taagccgcac gattctgaat tcgtcgattg gtagtcgact
     2761 ccgttcgtca tgcccgcgca ggcctacgtc cagactttat ttgactccct agtgcacgcg
     2821 aggacagagc ccggcttaca cccgtaaggt agtgcggagc acggatctct ttgcggtgtg
     2881 ccgtttagga caataccgct gtcgcttcga tctcgaccag atatttttca ccggcaaaac
     2941 catccacgaa agtaaactcg acctccggca cggttagtcg gtcggcggcg gcgagaacct
     3001 gtctgacctc ttggacattt gaaccgcgtt gcagcaataa cgataatttc tcgacgtttg
     3061 accaatcgga cttggcgcgg gcgaaggcgt tatccagtgt gcccagcaca tctttaacct
     3121 gctgagccaa tgttgccgcc tctgaggtaa agcccgagaa gaacagcagc gcgtcatatt
     3181 cgagatagca gagataagtg cgcgccggct cgaaatcgat cgggttgcgc tgaacggtcg
     3241 cgttcaaggg ccgcatcgcc aggagttcca atccacccga accgtcggaa tcgaaccatt
     3301 gtttcgatat gaaactcgag ctggcggcgc ggcgcttgcc gctgagaatt tttgcgcgcg
     3361 ccacggtcgc atcggtacgc gcttgcctgt cgcgcccata gacgcgaatg cgcgccgtat
     3421 tatctagcga caagccattg gacttgagcg cggtgtcgaa acgttcgaat aattccgccg
     3481 tcgcgctctc aaccgacgac ccggccgtcg cttctccggt tatttcaagg aactcgcgtc
     3541 caagccacgt gaaaatcttg gtttccatag tggcggtaac ataccgcaac acgataaccg
     3601 agacaaagcc attctagatc cagtcttgtc ttagccttct aatctgtcat ttcgagcgaa
     3661 gcgagaaatc tcttccacgc ttcgcatcga tgtcaaactc aagcgtacaa ttccactcac
     3721 gccttgcgtt tggccgtcgg tttggcatat ctggcttagc catgtgaaga ggagaatcga
     3781 tgttagcgtc acgcctcacc aacacaacat tcgcactgct ggtctcgctg ttatttgtta
     3841 ctgccgcgtt cgcccaaccg accaagattc gcctggcctc tacggctgca accagcacgg
     3901 aaaaaatcgc cttcgccttg gccaaggatg ccggcattct caaaaaacac aatatcgatt
     3961 tggaactgat tttgatcacg ggcgggccag tggcgatgca ggcattgctc gcgaagagcc
     4021 tagatataat caccaccagc gccaccgtgt ttctccatgg tttcgtcgaa ggcgccgatg
     4081 tcaagatcgt cggcggcgtc aacaaccgct ttccttatac gttcttcacg cgaccgaata
     4141 ttactgctcc ggctcaattg aaaggtaaaa ccttggggat tacgcgttac ggcagcaccg
     4201 acgagctcgc gacacgtatg gcgctcgagc agttcgggtt gaatcccaag agcgacgtca
     4261 aattcatcca aggcggaggc tcggccggcc gtatcaacgc tctccaagcc ggcagcatcg
     4321 atggcacctc gctcatatcc ggtgtcagtc atgtcgcgaa gaaagccggg atgaatcctc
     4381 tgctcgactt cgcggagaaa gagatcgact atcagatgac cggcgtggtc gcccgcggcg
     4441 acttcttaaa aagtagtccc gaggggctgc gccgcttcct gcgcgcctac gtcgaatcga
     4501 tccgcttcta taaagccaac cgcagcgaag caatcagaga aaccatgaag gcgatccgca
     4561 ccgatgatcg tcaactggcc gaagccgact acaactaccg cgcgcgtgcc ttccccgacg
     4621 acggcaagcc gacgttgaaa ggcatccagc tggccatcga cgagttggtg aaagagaacc
     4681 cgaaagcaaa aaccgttagc ccacagcaag tgatcgatct cagctttttg ccgtagaaaa
     4741 atgtttcgcc gcctcttcac cgccgaaggc cagagtcgtt ccgccagaac cgtagaaatt
     4801 ttcggctggc tgattctcgc ggaaggcact tcggtcgtgc tcttgccgca actcgccgtg
     4861 gggctgctgc atctaccgcc attggtcggg caagcggaga attatttccg cctcgtggga
     4921 atactggtga gcggtgtcgg catgctctat gttgtcagtg gccggctaaa cgccgaaggc
     4981 tttgtctttg cctccatgct cgatcgaccg ctagtccccg ttgcgatggc gatcctttgg
     5041 catttggaaa tcatacccgg caccctagcc ttaatttttt cgctacagga cttcggcagt
     5101 tttgtctgga cgctcgtcac gtggcggtcg gaacatagat gatgatcgta gcgagggagg
     5161 acatattatg agcaatccga tcataggttt catcggcttg ggcatcatgg gcaagcccat
     5221 ggcgcgccat cttatcaagg cgggctatcc gttggtgatt cacaaccgca gccgcgcggc
     5281 aatagacgaa ctaagtaaag aaggcgcgca gatggccgcg aattcgcaag aagtcgccca
     5341 gcgcagcgaa gttattatca ctatgctgcc cgactcacct gatgtcgaac tcgtctacgc
     5401 cgcggagcac ggcatttttg ccggagtgaa atcgggaacg cttctaatcg atatgtcgag
     5461 catttcgccg gtggtcgcgc gcaagctcgc ggcggaagcg gagaagcgcg gctgcgacat
     5521 gctcgatgcg ccggttagcg gcggcgaagc cggcgccatc gccgcatcgc tttcgatcat
     5581 gatcggcggc gaggctggcg cggtcgcgcg cgccatgccg atctttgaga agctcggtaa
     5641 aaatattgtc catgtcggcg ctgccggggc tggccaagtg accaaagcgg cgaaccagat
     5701 ggttgtcggt acgacgatcg ccatcgtcag cgaggcgctc gtgctggcgg caaaagccgg
     5761 cgtcgatccg gccaaggtca ggcaagcgct actcggcggc tttgcccaat cgaaaatcct
     5821 cgaggcccat ggacagaaaa tgctcgatcg caacttcaaa cccggcttc
//
LOCUS       DATLCH010000125         6925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4093586, whole genome shotgun sequence.
ACCESSION   DATLCH010000125 DATLCH010000000
VERSION     DATLCH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4093586"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VMZ02_03675"
     CDS             <1..1132
                     /locus_tag="VMZ02_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71061.1"
                     /translation="ESVIMKHAVVIVLAVICLSLAPQIFFAAESANPRAEWEKTVEAA
                     KKEGQLTLYHWGTPLMLDAGAFQKAYPEIKVTIVSAMGNDLMQRILAERRGEKFIPDV
                     YIAGIATMMVLHKAKTFDPIKTALILPEVSDESKWWRGKHTYGDAERAHIFTFTKSPD
                     YGSIGLNTKLVDPLEFKSYWDFLQPKWKSKITVQDLRGGGPGSTPLRFMYYNPELGAK
                     YLRQLYGGMDATLYRDNRLALDWLGSGKFALAFFVQKVEEAEIKGLPVLQFRQPMKEG
                     VGLSSRVGHMALLNRAPHPNAAKVFINWYLSRDGQELFQRLQIGAHDPADSLRIDIPK
                     NQIPPADRRQDGVKYIDLDEQKVFDPSPAIQLIKETLGDAGK"
     gene            1145..2263
                     /locus_tag="VMZ02_03680"
     CDS             1145..2263
                     /locus_tag="VMZ02_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71062.1"
                     /translation="MIKLPSRFLLFCALSLATSADAAELKPSSAEWEATLKAARAEKE
                     VAVGCEPTPESRNALMQFQQAFPDIQLKLNPIGARDFATRVLAERRAGKYLADVFSGG
                     TTSPSQVLVPANALDPIRTAFILPEVINETLWFKKRFHFADGENQFVFLNDATVNIDH
                     LVYNTNLVRADEIRSLWDMLQPKWKGKIVAYDPRLPGGASNNMRFIYYNPKLGPAFLQ
                     RLFSEMDVTIGSNNRQLMDWLAAGKYSLGLFIGRDIETALKQGLPVAEVNSTKADGAM
                     LASATGSIALINRAPHPNAAKVFINWFLSREGQMAWQKQTDRNSLRSDIPKDGITKWR
                     DKVPPDDGNYIFTNLPAYNDLKPGQKIVEEALAQAAKK"
     gene            2260..2529
                     /locus_tag="VMZ02_03685"
     CDS             2260..2529
                     /locus_tag="VMZ02_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71063.1"
                     /translation="MNKLTPAQTTKTAKTPACPECGATSVVPVIHGIPSTAQAKKIAA
                     GQAIAADREEWEGMSEWYCNACGCDWSRHSRRFKKPGGVNATRGY"
     gene            complement(2551..3267)
                     /locus_tag="VMZ02_03690"
     CDS             complement(2551..3267)
                     /locus_tag="VMZ02_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR71064.1"
                     /translation="MKLDVQLRMRLKAEDRLFQLEVAFQSAKSLIVIFGPSGAGKSVT
                     IQAIAGLIKPDSGRIVLDGRVLFDSKSAVNLAARERNVGYVFQDYALFPHLSVAQNVA
                     FPLEQSLRNGASGAAVEEFLAKFDLADLGTSYPRQLSGGQRQRVALARALIRKPDILL
                     LDEPLAALDPVLRGKTRGELLKLQQSFAVPMVVITHDPADVEAFAEDLIVFDIGKVAQ
                     QISIDSNRRASQVREVLGLG"
     gene            complement(3289..3966)
                     /gene="modB"
                     /locus_tag="VMZ02_03695"
     CDS             complement(3289..3966)
                     /gene="modB"
                     /locus_tag="VMZ02_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HUR71065.1"
                     /translation="MTSSPMTDALVPLLLTLKIAVAATAIVLFAGVACAYLLGRYSFW
                     GSQIIDAICTLPMVMPPTVLGYYLLVVFGRRGWIGAWLEESLGIHLIFTWQGAVIASA
                     AVAFPLVYRAARASLGSVDHHLENAARVLGAGEAKVFFRVSLPLAWRGVFAGTMLAFA
                     RAMGEFGATLMIAGSIPGKTQTISIAIYEAVQAGQDSVANFLVLLASLVCVCILAASG
                     KFLQRQF"
     gene            complement(3969..4643)
                     /gene="modA"
                     /locus_tag="VMZ02_03700"
     CDS             complement(3969..4643)
                     /gene="modA"
                     /locus_tag="VMZ02_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HUR71066.1"
                     /translation="MSAAASLTNAFKNIGAEFERVHARDKVIFNFAASDVLVKQIAEG
                     APVDVFASADEEAMDKALTAKLIIATTRFNFAGNRLVLALPMDSPLRFNTLDDLKTKA
                     VRRIAISQPATVPVGRYAKAVLDKARLWTVLQERFIYTQNVRQTLDYLARGEVDAGFV
                     YTTDAALMKDKVRVALEVTTTRRVVYPIAMTTASKNPQLGRDFLDLVRSSAGQTILQK
                     FAFTEP"
     gene            4865..5983
                     /locus_tag="VMZ02_03705"
     CDS             4865..5983
                     /locus_tag="VMZ02_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71067.1"
                     /translation="MRITPALMIVLASLIWLPTSAARAAEIKAPWQSEWEKTVEAAKK
                     EGKLVAAIPASAELRKAIGEVFPKRYPGIELDLTNARGPSNAAKIAAEHAAGVRDFDL
                     LISGTSTPFNLLNAGILEPAEPLLILPEVKDPKRWFGGHVWLDNAKKFIYAFQVYQSE
                     NIWHNAAMMKPEEARSYDDLLLPKFKGKIGILDPRSAGAGTATWAFFLKIKGEEWLKK
                     LAAQDLFLSRDQRQLADSLAKGKVAVTIGLTYYTFSPFLKAGLPVKPLPDMKEGTYTS
                     CGSSATSIVKNSPHPNATKVFINWLYGKEGQEIYGKAMGQATRRLDVDTKWMAAHGVR
                     ASKDFLSVEENNRLENYGEETVGKYWAASVKIAEEVFK"
     gene            6098..>6925
                     /locus_tag="VMZ02_03710"
     CDS             6098..>6925
                     /locus_tag="VMZ02_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71068.1"
                     /translation="MLKRHLILALLGSLLIPIMSARAQSEWDKTVEAAKKEGKIVAGI
                     PASAELRKALDDTFSKRYPAIDIELATGRGPANASKIAAEHAAGVRYFDVLISGSLTP
                     LNLLNAGILEPIEPLLIVPEVKDPKRWYGGHVWADNAKKYLYSFQAYQSENFWHNTQL
                     LKPEEYRAFDDLLNSKWKGKIAILDPRSAGGGTSSWAFFYKVKGEEFLRKLAAQELTL
                     SREQRLLGESLAKGRFAITIGLTYYSLAPFIKANLPIKPLPEAKEGSYTSSGSGALSV
                     "
BASE COUNT         1673 a         2066 c         1853 g         1333 t
ORIGIN      
        1 agaaagcgtc ataatgaagc acgcggttgt catagtctta gctgtcattt gtctctccct
       61 tgctccccaa atatttttcg cagctgaatc cgccaacccc cgcgctgaat gggaaaagac
      121 cgtggaggcc gccaagaagg aaggccaatt gactctctat cattggggca cgccgcttat
      181 gctcgatgcc ggcgcgtttc agaaggccta tccggaaatc aaagtcacca ttgtgagtgc
      241 catgggcaac gacctgatgc agcgcatttt agccgagcgc cgcggcgaaa agttcattcc
      301 agacgtctac atcgccggca tcgccaccat gatggtgctg cacaaagcga agactttcga
      361 cccgatcaag actgcgttga ttctgccgga agtaagcgac gagtccaaat ggtggcgcgg
      421 caagcatacc tacggtgacg ctgagcgggc gcatattttc accttcacca aaagccccga
      481 ctacggcagc atcggcctca ataccaagct cgtcgatccg ttggagttta aatcctattg
      541 ggattttctt cagcctaaat ggaaatcgaa aatcaccgtg caggatctgc gcggcggcgg
      601 accgggctcc acgccgctgc gcttcatgta ctacaatccc gaattgggcg ctaaatatct
      661 gcgccagctt tatggcggca tggatgcgac gctttatcgc gacaaccgtc tcgcccttga
      721 ctggctcggc agcggcaagt ttgccctggc ttttttcgtt caaaaggtcg aggaggcgga
      781 gatcaaaggc ctccccgtat tgcagtttag gcaacccatg aaggaaggcg tcgggctctc
      841 atcgcgcgtc ggccacatgg cgctgctcaa tcgcgcgccc catcccaacg cagccaaagt
      901 tttcatcaac tggtatttgt cgcgcgacgg ccaggagcta tttcagcggc tgcaaatcgg
      961 cgcccacgat cctgccgact ccctgcgcat cgatattccc aaaaaccaaa tcccgccagc
     1021 cgatcgccgc caggacggcg tcaagtatat cgatctcgac gagcaaaagg tcttcgatcc
     1081 ttcgccggcg attcaattga tcaaggaaac actcggtgat gccggcaagt aggagtgagc
     1141 cctcatgatt aaattaccct cgcggttttt gctgttctgc gccctctcgc tcgcgacttc
     1201 tgccgatgcc gccgaactga agccctcgtc cgccgaatgg gaagcgacgc tgaaagccgc
     1261 cagggcggaa aaggaagtgg cggtcggctg cgaacccacc ccggaatcgc gcaatgctct
     1321 gatgcagttt cagcaagcct tcccggatat tcaattgaag ttgaatccca tcggcgcccg
     1381 cgactttgcc acccgtgtgc tggcggagcg gcgcgccgga aaatatctcg ccgatgtttt
     1441 tagcggcggc accacctcac catcccaagt cctggttcca gccaatgccc tcgatccgat
     1501 ccgcaccgcc ttcattctgc ccgaggtcat caacgaaact ctctggttca agaaacgatt
     1561 tcatttcgct gacggcgaga atcagttcgt ttttttaaac gacgcaacgg ttaacatcga
     1621 ccatttggtc tacaacacca atctggtgcg cgctgacgag atccgctcgc tgtgggacat
     1681 gcttcagccc aagtggaaag gcaaaatcgt ggcctacgat ccgcgtcttc cgggcggcgc
     1741 cagcaacaac atgcgcttta tctactacaa ccccaagctt ggcccggcat tcttgcaacg
     1801 cttgtttagc gagatggacg tcaccatcgg ctcgaacaac cgtcaactta tggactggct
     1861 ggctgccggc aagtattcgt tgggcctgtt catcggccgc gatatcgaaa ccgcgctcaa
     1921 gcaaggattg cccgtggccg aagtaaactc aactaaagcc gacggcgcga tgctggcctc
     1981 tgccaccggc agcatcgcat tgatcaatcg cgcgccccat cccaatgccg ccaaggtttt
     2041 catcaactgg tttctgtccc gcgaaggaca aatggcctgg caaaaacaga ccgacagaaa
     2101 ctcgctgaga agcgatattc ccaaagacgg catcaccaaa tggcgcgaca aagtgccacc
     2161 cgacgacggc aactatatat ttaccaatct gcccgcttat aatgacctga agccggggca
     2221 aaagatcgtc gaagaggcgc tcgctcaagc agccaaaaaa tgaacaagct cacgcctgct
     2281 caaacaacga aaacagccaa aactcccgcg tgccccgaat gcggtgcaac atcggtggtc
     2341 ccggttattc acggcattcc aagcaccgcg caagcgaaga aaatcgccgc cggccaagcg
     2401 atcgccgcgg atcgcgaaga atgggaaggc atgagcgagt ggtactgcaa tgcctgcggc
     2461 tgcgattgga gccgccactc gcgccgattc aaaaaacccg gcggagtgaa cgcgacgcgc
     2521 ggatactgag acgattgccc caatcgaatg ctaaccgagc ccaagcactt cacgaacctg
     2581 tgaagcacgg cgattggaat cgatcgagat ctgctgcgcg accttcccga tatcgaaaac
     2641 aatcaaatct tccgcaaacg cttcaacgtc ggcgggatcg tgggtgatca cgaccatggg
     2701 caccgcgaaa ctttgttgca gcttgagcag ttcgccgcgc gttttgccgc gcaagaccgg
     2761 gtcgagagcc gcaagcggct cgtcgagaag caaaatatca ggcttacgga taagggcgcg
     2821 tgccaaggcg acgcgctggc gctggccgcc ggacagttgg cgtggatagc ttgtgcctag
     2881 atcggcgagg tcgaatttgg caagaaattc ctccaccgcc gcccctgacg cgccattccg
     2941 caaagactgc tccagcggga acgcgacgtt ttgcgccacg ctaaggtgag gaaataacgc
     3001 gtagtcttga aacacatagc cgacgttgcg ctcgcgtgcg gcaagattca cggcagattt
     3061 tgaatcgaac aggactcggc catcgaggac gattcgcccg ctgtccggtt tgatcaggcc
     3121 ggcgatcgcc tgaatggtca cactcttgcc ggcgccggac ggtccgaaga tgacgatcag
     3181 actcttggcg gattgaaaag cgacctcgag ttgaaaaagc cggtcttcgg ctttgaggcg
     3241 catgcgtagc tgcacgtcca gtttcataat ttcaaatcac cgcaacgctt agaactgccg
     3301 ctgcaaaaat ttgcccgacg cggccaaaat gcaaacgcaa accagcgaag ctaacagcac
     3361 caagaaattg gcgacgctgt cctggccggc ttgcaccgct tcatagatcg cgatggaaat
     3421 cgtttgagtc tttcccggga tgctgccggc gatcatcaag gttgcgccga actcgcccat
     3481 ggcgcgagcg aacgccaaca tggtgccggc aaaaacgcct cgccaggcca gcggcagact
     3541 gacgcgaaaa aacaccttcg cttcaccggc acccaagacc cgcgcggcat tttcgagatg
     3601 atgatcgacg ctgcccagcg aagctcgcgc cgctcggtac acaagtggaa aagcgacggc
     3661 ggcagaagcg atcaccgcgc cctgccaggt aaagatcaaa tgaataccta aactctcttc
     3721 caaccacgca ccgatccaac cgcgccgtcc aaagacgacc aggagataat agcccaatac
     3781 cgtgggcggc atgaccatcg gcaaggtgca gatcgcgtcg atgatctgac tcccccaaaa
     3841 cgaatagcgg cccagcagat aagcgcacgc cacgccggca aaaagcacaa tcgcggtcgc
     3901 cgcaacagcg atcttcagag tgagcaacaa cggaaccaac gcgtcagtca ttggggatga
     3961 ggtcacaatc acggctcggt gaaagcgaat ttctgcagga tggtttgacc agcgctggag
     4021 cgcactaagt cgaggaagtc acgcccgagc tgggggtttt tgctcgccgt cgtcatggca
     4081 atgggataca ccacccgccg ggtcgtcgtc acttccaacg cgacgcggac tttgtccttc
     4141 atcagcgcgg catccgtcgt gtaaacaaaa ccggcatcga cttcgccgcg cgccagatag
     4201 tcgagagttt ggcgcacgtt ctgggtgtaa atgaatcgtt cctgcaagac cgtccagagc
     4261 ctcgctttat cgagcaccgc cttggcataa cggccgaccg gaaccgttgc cggttgactg
     4321 atcgcgatcc gccgcactgc cttcgtcttc aagtcatcga gcgtgttaaa gcgcagcggt
     4381 gaatccatcg gcagcgccag caccaagcga ttgccggcaa aattaaacct ggtagtcgcg
     4441 atgatcagtt ttgctgtgag cgccttgtcc atcgcctctt catccgctga agcaaataca
     4501 tccaccggcg ctccctcggc aatctgctta accagcacat cggaggcggc aaagttaaaa
     4561 atcactttgt cgcgcgcatg cacgcgctcg aattctgcgc cgatgttttt gaatgcgttg
     4621 gtcaggctcg ccgctgccga cacccataat tctgtggccc cgacccgagg tgcgaaagcg
     4681 tgaatgctca aacatatagt taatgttaat cgcagcgcgc acataagttc ctttatatag
     4741 catggttgca acggcgatcc attatcatca gatgagccag cgtcagtgca acacatagga
     4801 aattcagttg actcggaatg atgcctgaga caaattggct caacttcact gcgacgaggt
     4861 gccgatgcga attacgcctg cgttaatgat tgtactggct tcacttattt ggcttcccac
     4921 cagtgccgcg cgagctgccg aaattaaagc accgtggcaa agcgagtggg aaaagaccgt
     4981 cgaagcggca aagaaggaag gcaaattggt cgccgcgatt cccgccagcg ccgagctgcg
     5041 caaggcgatt ggcgaggtgt ttcccaagcg ctatccgggc atcgagctcg atctcaccaa
     5101 cgcacgcggc ccatccaacg ccgccaagat tgccgccgaa cacgccgctg gagtgcgcga
     5161 ctttgatcta ctgatcagcg gcacgtcgac gcccttcaat ttgctcaacg cgggtattct
     5221 ggaaccggcc gaaccgctgc tgattctgcc cgaagtcaaa gatcccaagc gctggtttgg
     5281 cggccatgtt tggctcgaca acgccaagaa atttatctac gcctttcagg tctatcagtc
     5341 ggaaaacatc tggcacaacg cggcgatgat gaaacccgag gaggcacggt cctatgatga
     5401 cctgctgtta ccgaagttca aaggtaagat cggcattctt gacccacgca gcgccggcgc
     5461 aggcacggcg acctgggcgt tctttctaaa aatcaaaggc gaagagtggc tgaaaaaact
     5521 cgcggcacaa gatctgtttc tatcgcgcga tcaacgccag ctcgccgaca gcctggcaaa
     5581 gggcaaggtc gccgtcacca tcggcctgac ctactataca ttttcaccgt tcttgaaggc
     5641 cggactgcca gtcaaaccgc tccccgatat gaaagaaggc acctacacca gctgcggcag
     5701 cagcgcgacc tcgatcgtga aaaattcgcc gcaccccaac gcaaccaaag tctttatcaa
     5761 ctggctctac ggcaaagaag gccaggaaat ttacggtaag gccatgggac aagccacccg
     5821 ccgcctcgat gtcgatacca aatggatggc ggcacacggt gtgcgggcgt caaaagattt
     5881 tctctccgtc gaagagaaca atcgcctgga aaactacggc gaagaaactg tcggcaaata
     5941 ctgggcggca tcggtaaaga tcgcggaaga agttttcaaa tagcgaggcg cggaacgcgc
     6001 ccgttcaaag ttcaagggtt caggttcaaa gttcggacat cctcatcccc gctgaccttg
     6061 aacttttgaa ccttgaacca gcgcgcgcgt ggcacaaatg ctaaaacgcc atctaatact
     6121 cgcactactc ggcagtctgc taataccgat catgagcgcg cgcgcccaat cggaatggga
     6181 caagaccgtc gaggccgcca agaaggaagg caagatcgtc gccggcattc cggcgagcgc
     6241 ggagctgcgc aaagcgctcg atgatacttt cagcaagcgc tatccggcca tcgacatcga
     6301 gctggccacc ggccgcgggc cggccaacgc cagcaagatt gccgccgaac atgccgccgg
     6361 cgtgcgctac ttcgacgtgc tgatcagcgg ctctttgacg ccgttgaacc tgttgaatgc
     6421 cggcattctc gagcccatcg aaccgctatt gatcgtgccg gaagtgaaag atcccaagcg
     6481 ctggtacggc ggccatgtct gggccgacaa cgccaaaaaa tatctctact cattccaggc
     6541 ctaccagtcg gaaaattttt ggcacaatac ccaattatta aagcccgaag agtatcgcgc
     6601 ctttgacgac ctgttaaatt caaagtggaa aggcaagatc gccatcctcg atccgcgttc
     6661 cgccggcggc ggcacatcga gctgggcgtt tttttacaaa gttaaaggtg aagaattttt
     6721 gcgcaagctg gccgctcagg agctgaccct gtcgcgtgag cagcggctgc ttggcgagag
     6781 cctcgccaag ggacgctttg ccatcaccat cggcctcacc tactactcgt tggcgccatt
     6841 catcaaagcc aacttgccga tcaagcctct gcccgaagcc aaagaaggca gctacacgag
     6901 cagcggaagc ggcgctctat cggtg
//
LOCUS       DATLCH010000126         6612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7643722, whole genome shotgun sequence.
ACCESSION   DATLCH010000126 DATLCH010000000
VERSION     DATLCH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7643722"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VMZ02_03715"
     CDS             <1..815
                     /locus_tag="VMZ02_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR71069.1"
                     /translation="MLNKGRWGNHNTSLRGSQGERLEETKLYEYGDPMNVNISETILN
                     ALEKRKPGEALKIRPEDFAVNRVEYSTMSETALLIDVSYSMLMNDALHAGKKVALALH
                     RLIQTKYPQDTLHLVAFRSNAKLIQAEDLPSIVSLTYFMEHGTDIKEALRYARQLLGS
                     NRSANRQIILITDGEPTAASLDRGGRLHSGWGSAMLHGRIVHETLKEVKRCTQSGIKI
                     NTFMLGADYYRQGFVDQLSRLNIGRVFYTTPDQMGNYIMVDYLNNKKKRVGR"
     gene            complement(929..2107)
                     /locus_tag="VMZ02_03720"
     CDS             complement(929..2107)
                     /locus_tag="VMZ02_03720"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HUR71070.1"
                     /translation="MLRAGAGQSNNPSTAQAVSEAAQLAMAQAGVGRADGGVIFFTAA
                     HAAHGQEMVTTLAKIIGTDCVTGASGAGILTGAGEIEGDHGIAVLVFATDRFFARPFL
                     FEPLRGNETNLGETFGGFLAKAQDEKSLMLLFPDTYNGDAQTLLQAIEARAGFHAIAG
                     AGATENGAAGATYQLCGDKLASNSLAGMYISGKFETHIDITQGCQPITAPMTITKAAN
                     NLIHEIDHQPAFEVFAKLLKGPLADDLRRALSVLFVGLPADRSENSVAAGKYLVRNIV
                     GLDPEKGIVAVADKVSEGQEMIFALRDGQRARDDLQEMLQRQLLHLHGKTPSFGFYFN
                     CCARGSSLYGIPGIDAAYIKQVLGDFPFVGMFGGYELAPLGRANHLFAYTGVLVLVTE
                     "
     gene            2292..3059
                     /gene="gloB"
                     /locus_tag="VMZ02_03725"
     CDS             2292..3059
                     /gene="gloB"
                     /locus_tag="VMZ02_03725"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HUR71071.1"
                     /translation="MKIHQIPLLRDNYGYLIVCEQTNQAAIIDPSEADPVLRIIEQEK
                     VEPVAILNTHHHRDHTGGNEAFLAKHPVKVYGHTSDRSRIFGLTDGVDEGDTIQVGER
                     TGKVFFIPGHTTGHVAYLFDDNLFCGDTLFTAGCGRLFEGTPEQMQNSLKKLMSLPDN
                     TNVYCGHEYTESNLRFAMTVEGTNPQLAARFERVQGLRARGLPTVPSTMEEEKQTNPF
                     LRWNSKEIQASVKAVSPDNPVDPVSIFAMVRKMKDSF"
     gene            3102..3584
                     /locus_tag="VMZ02_03730"
     CDS             3102..3584
                     /locus_tag="VMZ02_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HUR71072.1"
                     /translation="MNLKERTYLWYLSILRIYAGYYLFFQGIGKFQRKFAQGDWIGRQ
                     IGDVATLDLYPWYKTFLQRYVVPHSELFGYLVMIGEIAVGICLLLGLFTRVSAFIGLV
                     MLVNYYLGPGMARGGYMLAYQQFYIIALALFVLAGPGRTLGLDGLLFRGATAKGKDSK
                     "
     gene            3581..4708
                     /locus_tag="VMZ02_03735"
     CDS             3581..4708
                     /locus_tag="VMZ02_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUR71073.1"
                     /translation="MKLPGQASPEGTDRYRKRFTEKLPDEHFRQSQNLWLSSIGLGTY
                     LGNYDDATDQEYHRAVVIAVGAGCNVIDSAINYRLQRSERSIGTALKELAGRGYNREE
                     IVVATKGGFIPYDGTPPKEPRQYLEDTFIKTGLADAADIVSGCHCMAPKYLLNQLECS
                     LRNLDVETVDIYYVHNPETQLGKVTAAEFDSRLIKAFETLENAVAAGRTQMYGMASWN
                     CFRNAVDAKDYLSLAHVVELAEQAGGKDHHFKVIQLPLNLGMSEALSNANQPAHGHQH
                     NVLEVAQDLGLTVMCSASILQGQLSRDLPPLIAETFAGLESDAQRALQFVRSTPGVTT
                     ALVGMKQEKHVVENLAAARIAPASWEQYSKLFQAKDSVEPT"
     gene            complement(4740..5684)
                     /locus_tag="VMZ02_03740"
     CDS             complement(4740..5684)
                     /locus_tag="VMZ02_03740"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUR71074.1"
                     /translation="MDYTTLGKTGLKVSVAGLGCGGPSRLGMRGDAGAEEHAVRLIKQ
                     AIDLGVNFLDTAQNYGTENVVGKAIADMPRDRVVISTKKTLPSADEPNPEAAIVEGLE
                     KSLKLLGTDYIDIYHLHGVEPKDYEFAQRRLIEPMRRLQEQGKIRFVGVTEGFVVDTT
                     HTMLADSLKKNLWDVIMVGFSLLNPSARKNVFPLTLKRGVGVLDMFAVRRALSQPQRL
                     KEMCAELVEKGMVVRDGLDLEDPLGFLLKETDATTLPEAAYRFCRHEQGVDVVLTGTG
                     NPDHLQENVAAILKPPLAKSALAKLERLFGGLDFLTGN"
     gene            complement(5787..6242)
                     /locus_tag="VMZ02_03745"
     CDS             complement(5787..6242)
                     /locus_tag="VMZ02_03745"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HUR71075.1"
                     /translation="MSVRAFQDQGSVHHCYGCGAANEKGLRLKSYWDGDEAVAIWRAE
                     PHHCGGTTENLNGGIIASLIDCHSLNLAIAQAYRAAGRLIGSAPRIGYVTANLNVAYL
                     KPTPLREQIDLRARIAKLDGRKAWVHCTLSVAEIVRAKGEVLGVRVEWD"
     gene            complement(6244..>6612)
                     /gene="msrB"
                     /locus_tag="VMZ02_03750"
     CDS             complement(6244..>6612)
                     /gene="msrB"
                     /locus_tag="VMZ02_03750"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HUR71076.1"
                     /translation="EWKKQLTEEQFQVARKKGTERAFTGAYWDNHEKGTYRCVCCDNE
                     LFSSETKFDSGTGWPSFWAPVAADNVETDEDNSYGMRRVEAVCSKCGAHLGHVFDDGP
                     KPTELRYCINSASLKFAKDK"
BASE COUNT         1502 a         1926 c         1752 g         1432 t
ORIGIN      
        1 agatgctcaa taaaggacgc tggggtaacc ataatacctc cctgcgcggc tctcagggcg
       61 aacgcttgga agaaaccaag ctgtacgagt atggcgaccc gatgaacgtc aatattagcg
      121 agacgatttt gaatgctttg gaaaagcgca agcccggcga agcgctgaaa attcgtcccg
      181 aagacttcgc ggtcaatcgc gtcgagtatt cgacgatgag cgaaaccgcg ttactgatcg
      241 atgtcagcta ctcgatgctg atgaacgacg cgctccacgc cggcaaaaaa gttgcgctgg
      301 ctctgcaccg gttgattcag accaagtatc cccaggacac attacacctg gtcgcgtttc
      361 gctccaacgc caaactgatt caggccgagg atctcccgtc gattgtctcg ctcacctact
      421 tcatggaaca cggcaccgac atcaaagaag ccttgcgtta tgcgcgccag ctgctgggct
      481 ccaaccgcag tgccaatcgg cagatcatct taattaccga tggagagccg acggcggcca
      541 gtctcgaccg cggcggccgg ctgcacagtg gctggggttc ggccatgctg catggccgga
      601 ttgttcacga aaccctgaaa gaagtgaagc gctgcaccca aagcggcatc aagatcaaca
      661 cctttatgct cggcgccgat tactaccgcc aaggctttgt cgaccaactc tcgcgtttga
      721 acatcggccg ggtcttctac acgactcccg accagatggg caactacatc atggtcgatt
      781 acctcaataa caagaaaaaa cgcgtcggtc ggtaactcac cgccaatttc aaaatttaga
      841 tcgtcaaccg cacgtgtatc gaatcggaaa cttggcagcc caagtcctct gacgttgaac
      901 ctcgaagttt tgaaccttga cgagtgattt attcagtcac caacaccaac acgccggtgt
      961 aggcaaacaa gtgattggcg cgcccgagcg gcgcaagctc atagccgccg aacatgccga
     1021 caaagggaaa gtcgcccagc acctgtttga tgtaggcggc gtcgatcccg ggaatgccat
     1081 agagcgaact gccgcgggcg cagcagttaa aatagaaacc gaaggacggt gttttgccgt
     1141 gcagatgcag cagctggcgt tgcagcatct cctgcaaatc atcgcgcgcg cgctggccat
     1201 cgcgcagggc aaaaatcatc tcctgcccct cgctcacctt gtcggccact gcaacgatgc
     1261 ctttttcagg atccaagccg acaatatttc gcaccagata ttttcccgcg gcgacgctgt
     1321 tctcactgcg atcggccggc aggccgacaa acagcacgct gagcgcgcgg cgcagatcgt
     1381 ccgccagcgg ccccttgagc agcttggcaa atacttcgaa tgctggctga tgatcgattt
     1441 catgaatcaa gttatttgct gccttggtga ttgtcatggg cgccgtgatc ggctggcagc
     1501 cttgagttat gtcgatatga gtttcgaatt tgccggagat atacatgccc gctagggagt
     1561 tactggccag cttgtcgccg cataactggt aggtcgcgcc ggcggcgcca ttctccgttg
     1621 cgcccgcgcc ggcgatggca tgaaaacccg cgcgtgcttc gatcgcctgc aggagggttt
     1681 gcgcatcgcc gttgtaggtg tcggggaaga gaagcatcag cgatttctca tcttgagcct
     1741 tggcgagaaa gccgccaaag gtttcaccca agttagtctc gttgccgcgc agcggttcga
     1801 agagaaatgg ccgggcaaaa aatcggtcgg tggcgaacac cagcacggca atcccatgat
     1861 cgccttcgat ctcgccggca ccggtcaata ttccagcgcc gctcgcaccg gtaacgcagt
     1921 ccgtgccaat gattttggcc aaggtggtca ccatctcctg accatgcgca gcgtgagcgg
     1981 cggtaaagaa aatcacgccg ccatcggctc ggccaacgcc cgcttgcgcc atcgctagct
     2041 gagccgcttc actgacagct tgcgcggttg aaggattgtt tgattgtccg gcaccggccc
     2101 gtagcatgat gccggtacta taaagaaacg ttgagcgcag ggtcaagcgg attaagcaac
     2161 ctcggcgcgg gccatcaatg ttgaacaatt gtgctgatcg ccttgggcgt ggacttgttt
     2221 attccacatc gcaacgctat ttgaaaagat gctctgttcc aactaatatc cagctgcgca
     2281 ttggaaaaac aatgaagatc catcaaatcc cgctcttgcg tgacaactac ggttatctga
     2341 tcgtctgcga gcagaccaat caagccgcca tcatcgaccc ttcggaagcc gatccagtgc
     2401 tgcgaattat cgaacaagaa aaagtcgagc cagtggcgat cttgaatacg caccatcatc
     2461 gcgatcatac cggcggcaat gaagcattcc tggccaagca tccagtgaaa gtctacgggc
     2521 atacaagtga tcggtcgcga atctttggat taactgacgg cgtcgatgaa ggcgacacga
     2581 ttcaggtcgg cgaacgcacg ggcaaggtgt tttttattcc cggccatacc accggacacg
     2641 tcgcttatct atttgacgat aatctttttt gcggcgacac gctgttcacc gccggctgcg
     2701 gcagactttt cgaaggaacc ccggagcaaa tgcaaaattc gctaaaaaaa cttatgtcgc
     2761 tgcccgacaa caccaacgtt tactgcggcc atgaatatac cgaaagtaat ttgcgctttg
     2821 cgatgacggt ggaggggaca aatccacaac tggcggcgcg cttcgagcgt gtccaggggc
     2881 tgcgcgcccg cggtctccca acagtgccgt cgacaatgga agaggaaaaa caaaccaatc
     2941 catttttgcg ctggaatagc aaagaaatcc aagccagtgt gaaagccgtc tctcccgaca
     3001 accctgtcga tccggtatcg atattcgcga tggtgcgcaa aatgaaagat tcgttttagc
     3061 tcggcaagtg ccccggttgc gcattcgatt ctaagagcgc tgtgaatctc aaagaacgaa
     3121 cctacctgtg gtatctctcc atcctgcgaa tttacgcggg ttattatttg tttttccaag
     3181 gcatcggcaa atttcagcgc aaatttgccc agggcgattg gatcggccgg cagatcggcg
     3241 acgtcgctac tctcgacctt tatccttggt acaaaacctt cttgcaacga tatgtcgtgc
     3301 cacacagcga gctgttcggt tatttggtta tgatcggtga aatcgcggtg ggcatctgcc
     3361 tgctcttggg tttgtttact cgcgtgagcg cgtttatcgg gctcgtcatg ctggttaact
     3421 attacctcgg ccccggcatg gcacgcggcg gctacatgct cgcgtatcag caattctata
     3481 ttatcgcgct ggcactcttc gtactcgccg gtcctggtcg cacactgggc ctcgacggcc
     3541 tgctgtttcg cggtgccact gccaaaggaa aagactcgaa atgaaacttc ccggacaggc
     3601 gtcacctgaa ggcaccgacc gctaccgcaa acgtttcact gaaaaactgc ccgacgaaca
     3661 ttttcgccag tcgcaaaatc tttggctatc gtccatcggt ctcggcacct acttggggaa
     3721 ctatgacgac gcgacagatc aggaatatca ccgagcagtg gttatagcag tcggagccgg
     3781 ttgtaacgtc atcgacagcg ccatcaacta tcgcctgcaa cgcagcgaac gctccatcgg
     3841 gacggcgctc aaagaactcg ccggccgagg ttataatcgc gaagaaatcg tcgtcgccac
     3901 caagggcggc tttattcctt atgacggcac gccacccaag gaaccgcgcc agtatcttga
     3961 ggacactttt atcaaaactg gccttgccga cgccgccgac atcgtcagcg ggtgccactg
     4021 catggcaccc aagtatttac tcaaccagct cgaatgtagt ctgcgcaatc tcgacgtcga
     4081 aacagtcgac atttattacg tgcacaatcc ggaaacacag ctggggaaag tgactgccgc
     4141 agaattcgac agccgcctga tcaaggcgtt cgagacgctg gaaaatgccg tggcggcagg
     4201 aagaacgcag atgtacggca tggcgagttg gaactgcttt cgcaatgcgg ttgatgccaa
     4261 agattatcta tcgctggccc atgtcgtcga attggccgaa caggcgggcg gtaaagacca
     4321 ccatttcaaa gttattcaac tgccgctcaa cttgggcatg agcgaagcgt tgagcaatgc
     4381 caatcagccg gcccatggcc accagcacaa cgtgctcgaa gtcgcccagg atcttggtct
     4441 tacagtgatg tgcagtgcgt caatcttgca gggccagctc agtcgcgatt taccgccgct
     4501 aatcgctgaa acctttgccg gcctggaaag cgatgcccaa cgcgccttgc agttcgtccg
     4561 ctccacgccc ggcgtcacca ctgcgctcgt cggcatgaaa caagaaaaac atgtcgttga
     4621 gaaccttgcc gcggcgcgca tcgcaccggc ttcctgggaa cagtactcga agctgtttca
     4681 agcgaaagac tcggtcgaac cgacttagta gaactttccc ctcgtcccgg aagctcacct
     4741 tagttcccgg tcaagaagtc caatccgccg aacagtctct cgagcttcgc caacgccgat
     4801 ttggccagcg gcggtttcag aatcgccgca acgttctctt gcaaatggtc gggattacca
     4861 gtgcccgtaa ggacgacgtc gactccctgc tcatggcggc aaaaacggta agcagcttcg
     4921 ggcaacgtgg tggcgtcggt ttctttgagc aaaaaaccca gcggatcttc aagatccaag
     4981 ccatcgcgaa cgaccatgcc tttctcgacc agctcggcgc acatttcctt tagccgctgg
     5041 ggctggctga gggcgcgccg cacggcgaac atgtcgagca cgccgacccc gcgctttaaa
     5101 gtcagcggaa agacattttt tcgcgccgac ggattgagca ggctgaaacc gaccataatc
     5161 acgtcccaaa gatttttctt gaggctatcg gccagcatgg tgtgagtcgt atcgacaaca
     5221 aatccttcgg tgaccccgac aaagcgaatt ttaccctgct cctggaggcg gcgcatcggc
     5281 tcgatcagac ggcgttgcgc gaactcataa tccttcggct caaccccgtg taaatgataa
     5341 atgtcgatgt agtcggtgcc cagcagctta agactctttt ccagtccttc gacaatcgcc
     5401 gcctctggat tcggttcatc ggcgctcggc aaagttttct tggtcgatat tacgacccga
     5461 tcgcgcggca tatcggcgat cgctttgccg acgacatttt cggtgccgta attctgcgca
     5521 gtatcgagaa agttgacgcc gagatcgatc gcttgtttga tcagccgcac cgcgtgctct
     5581 tccgcaccgg catcaccacg catgccgagg cggctcggac ccccgcagcc caatccagcg
     5641 acgctcacct tcaatcccgt tttaccaagt gtggtgtagt ccatctaaga attctcgctc
     5701 cgttcaacgg ttgaacgttc aaggttggca gattcgcctg cgagtccgaa acgttgaact
     5761 tttgaacctt gaactttgaa cagcttttaa tcccattcca cccgcacacc caagacctca
     5821 cccttcgcgc gtacgatctc cgctacgctg agggtgcaat ggacccaggc tttgcgtccg
     5881 tcgagtttgg cgatacgggc acgcaaatcg atttgttccc gcagcggcgt aggtttcaga
     5941 taggcaacgt tgaggttcgc tgtcacatag ccaatcctgg gggcgctgcc gatcaggcgg
     6001 ccggcggcgc ggtaggcctg cgcaatcgcg agattcaagc tatgacaatc gatcagcgat
     6061 gcgatgatcc cgccattgag attttccgtc gtgccaccgc agtggtgcgg ctcggcacgc
     6121 caaatggcaa ccgcctcatc accgtcccag tagcttttca gccgcaaccc tttttcgttt
     6181 gcagcaccgc agccgtaaca gtgatgcacc gagccttggt cctgaaaagc gcgcacgctc
     6241 acgctacttg tctttggcaa atttcagcga cgcggaattg atgcaatagc gcagctcggt
     6301 aggcttgggc ccatcgtcga agacgtggcc caaatgggcg ccgcacttgc tgcaaacggc
     6361 ttccacccgg cgcatgccgt aactattgtc ctcgtcggtt tcgacgttgt cggccgccac
     6421 gggcgcccaa aagctcggcc aaccggtgcc ggagtcgaat ttcgtctccg aactgaacag
     6481 ctcgttgtcg cagcaaacac agcggtaggt gcccttctcg tggttgtccc agtaggcacc
     6541 ggtgaaagcc cgctcggtgc ctttcttgcg agccacttgg aactgttctt cagtcagttg
     6601 ttttttccat tc
//
LOCUS       DATLCH010000127         5440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_389009, whole genome shotgun sequence.
ACCESSION   DATLCH010000127 DATLCH010000000
VERSION     DATLCH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389009"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VMZ02_03755"
     CDS             <1..1266
                     /locus_tag="VMZ02_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HUR71077.1"
                     /translation="VRAHLMSDVPLGVFLSGGLDSSFIAALMAKHMNAPVKTFSVGFE
                     EKYYSEFDYSRIVARHIGAEHHEVTLTPKVLFDTLPKLIWHEDEPLKGAASVALYFVS
                     QLAKRHVKVVLTGEGSDELFAGYNHRYWGTLVARRLARWGGRLIPDIARRKIIRKMLW
                     ELPIPLPLKKKISHTPLYLSDTSEGLFFDNFHSIFTRDMQHELLDANLSRGLTDCDPY
                     ENALAILNSSKSDSFLHKMLYTDLNTDLVELLMKQDQMSMAASLESRVPFLDHVLVEF
                     AGTLPPQLRLQGRSGKWLVKKAAEAFLPKQIIHRPKMGFPVPFENWIKEDQSGFISDV
                     LLGSSAKSRGYFNPSYIEKLLHWHRAGMRDCHSQIWMLLNFELWQRTFFDGHAQEFDK
                     TPEISACGSGSHVGVHGRFSRTTGNFQAD"
     gene            complement(1276..3321)
                     /locus_tag="VMZ02_03760"
     CDS             complement(1276..3321)
                     /locus_tag="VMZ02_03760"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HUR71078.1"
                     /translation="MINRFVVQRRERFAGGHEFGITGAYEKLVGMIYGEVDPKHRRNR
                     IIVNVEKAPRNQRGRVEYSGNFCILKPLDMARGNGKIFFDAPNRGSKRILGFLNDAPE
                     ANDPTTLEDAGNGFLMRHGYTIVWCGWQGDLMPLKNWLVLNVPVATNTVKPIIATVRT
                     EIVVDEKGVKSQPLSGDERVKSYETASRDKSKAMLTVREKSYGKRIPIPANEWEYGAA
                     AKTPHSGNEIIKASGTDLYLRAGYKPGYIYEFIYQAKNPLVLGLGFAVVRDFVSFLRF
                     ESKDRAGKANPLRPPLSVRDGGRTSGGITHAYAWGRSQSGRYLRDFIYHGFNIYHGFN
                     EDESGHKVFDAIAPHVAGGGRLYLNYEFARPVTSSQQHTNQLDPELFPHAYNIIKDAQ
                     SGRHDGILKRPKTDPLVCHTQTSTEYWQKRGCLAHTDGRGDDLTLPDNVRLYVIASAQ
                     HNSPFGSEPTKDGSQFLVNPLPAGDILRALTVAMDLWATKDIPPPPSRYPTVKDGTLV
                     PPTRNGFPKIPGVKFVGRHNRQLFLDYGKHILSGKIETHPPKPLGKAYEILVPKADAD
                     GNDIAGIRLPAIVVPIGTYTGWNLRPRGRAEGELSGLLGSFMPFPTTKSQRRKTGDPR
                     LSLEERYDDNNDYVHQVSRASRILVEARYLLAEDAERMILEAKQRRLYAHSKTAVRL"
     gene            complement(3378..4322)
                     /locus_tag="VMZ02_03765"
     CDS             complement(3378..4322)
                     /locus_tag="VMZ02_03765"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HUR71079.1"
                     /translation="MPLVRIISTGGTIANTGHGLIAIDQVLGDIPQAKTMADFEVIEA
                     TRVRSGQMRLAQWLDIARAATAAAEDARVDGIIVTHGTFTTEETAYFLHLCVHTEKPL
                     VIVASQRKHDEVGNDGDRNLLDAIRLALTPEARGKGVLVTLHEEIHSAREVVKTNQRP
                     GGFHSPARGMLGHIEDDQVSFYATPTRRHTFHSEFDIRDIGELPRVDVIAAYVGGDDA
                     AAQACVAAGARGLVISGYAYNGRPSADQLGGVEKIAASGIPVVLASRGGQGRIPVDWN
                     DPFIQGDSLIPHKARILLMLGLTRTKDPKDLQRIFNEY"
     gene            4514..>5440
                     /locus_tag="VMZ02_03770"
     CDS             4514..>5440
                     /locus_tag="VMZ02_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71080.1"
                     /translation="MGKQVHAVLASILISLSCASLLWAAEVKRPPEWDKIVDAGKKEA
                     KVVIAIPPSNELRKELETVLKQKFAIEAELIAAPGPKNASRIAAEKKAGVNYFDAIIC
                     GTGTAAGLTHDGMLEPMESFWVLPEVKDAKQWWGGHIWEDNVSTNKFLYSFLADVATH
                     GIWYNTSLAKADELKSFDDYLNPKWKSKIGFSDPRVPSSGQSIWSFMWELKGEEFLKK
                     LVQQELFLTRDLRQLADALAKGKVAMAFGLGRSQTDPFVDAGLPIKPVPAPKEGLPAS
                     NSFGVIGIIKNPPNPNATKVFVNWFLSREGQEW"
BASE COUNT         1160 a         1413 c         1599 g         1268 t
ORIGIN      
        1 gtgcgcgcgc acctgatgag cgatgttccg ctaggggtgt ttttgagtgg tggattggac
       61 tcaagtttta tcgccgcgct gatggcgaag cacatgaatg cgccagtcaa aacgttctcc
      121 gtcgggtttg aagagaagta ctacagcgaa tttgattatt cgcgaatcgt ggcccgtcat
      181 atcggcgccg aacatcacga agttacgctc accccaaagg ttttgttcga cacgcttccc
      241 aaactcattt ggcatgaaga tgagcctctc aaaggcgccg ccagcgttgc cttgtatttc
      301 gtttcgcaat tggcaaagcg tcacgtcaag gttgtgctaa ccggtgaggg aagtgatgaa
      361 ctttttgccg gatacaacca tcgttactgg ggaactttgg tggctcggcg gttggcgcgg
      421 tggggcggcc ggctaattcc cgacatcgca agacgaaaaa ttatacgcaa gatgctctgg
      481 gaacttccaa taccgctgcc gctgaagaaa aaaatcagcc atactccgct ttatttgtcg
      541 gacacctcgg aaggactttt ttttgacaat tttcacagta tttttacccg cgacatgcaa
      601 catgagttgc tcgacgctaa tttatctcgt ggcctgacag actgtgatcc ttacgagaat
      661 gccttggcga ttttgaatag ctcaaagtcg gattcattcc tgcataaaat gttgtatacg
      721 gatttgaata cagatctcgt cgaattgctc atgaagcagg atcaaatgag catggcggca
      781 tcgttggaga gccgcgtgcc attcctggat catgtgctcg tcgagttcgc tggaacattg
      841 ccgcctcagc tgcgcctgca aggccgttcc gggaaatggc ttgtgaaaaa ggccgcggag
      901 gcgtttctac cgaaacagat cattcatcgc ccgaagatgg gctttccggt gccgttcgaa
      961 aattggatca aagaagacca gagcggtttt atcagcgatg tgcttttggg ttcgagcgcg
     1021 aagtcgcgtg gttacttcaa cccgtcttac atcgagaaat tactgcattg gcaccgtgcc
     1081 ggcatgcgtg actgccacag ccaaatatgg atgctgctca atttcgaact ttggcagcga
     1141 acttttttcg atggccacgc gcaggaattc gacaaaaccc cggagatttc tgcctgcggc
     1201 agcggcagcc atgttggcgt ccacggaaga ttctcacgca ctactggcaa tttccaagca
     1261 gactgaacgc cagtctcaca atcgcacagc tgtcttgcta tgggcatata aacgtcgctg
     1321 ttttgcttcg agaatcatac gttcagcatc ttcggccagt agatagcggg cttcgacaag
     1381 gatgcgcgac gctcgactca cttggtgcac atagtcgtta ttgtcatcgt agcgctcttc
     1441 gagggatagt cgcggatcgc cggtcttgcg ccgttgcgat ttggtcgtgg ggaatggcat
     1501 gaatgagccg agcaagccgg acagttcgcc ttctgcgcgg ccgcgtggcc gcaagttcca
     1561 tccagtgtaa gttccgatag gcactacgat ggccggcaga cgaatgccgg cgatatcatt
     1621 gccgtcggcg tccgccttgg gcacgagaat ttcataagcc ttgccgagcg gtttcggcgg
     1681 atgggtttcg atcttgccgc tcaagatatg ttttccgtag tcgagaaata gctggcggtt
     1741 gtgccggccg acgaatttca cgccgggaat ttttggaaag ccgttgcgtg tcggcggcac
     1801 cagggtccca tccttgacgg ttgggtagcg gctcggcggc ggcggaatat ctttcgtcgc
     1861 ccacaaatcc atggcgaccg tcagggcgcg cagaatgtcg ccagccggta acggattgac
     1921 cagaaactgg ctgccgtctt tggtcggctc ggagccgaat gggctgttgt gttgggcact
     1981 ggcaataacg tagagacgga cgttatcggg taaggtcagg tcgtcgcctc tgccgtccgt
     2041 gtgcgccagg cagccgcgct tttgccaata ctccgttgaa gtctgagtgt ggcaaacaag
     2101 cggatcggtt ttcggacgtt tcaatatgcc gtcgtgtcgc ccactctggg cgtctttgat
     2161 gatgttatag gcgtgcggaa agagttccgg gtcgagctgg ttggtgtgtt gttgcgatga
     2221 tgttaccgga cgagcgaatt cataattcaa atacaaccgg ccgccgccgg cgacatgagg
     2281 tgcaatggcg tcgaatactt tgtgccccga ctcgtcttcg ttgaaaccgt ggtagatgtt
     2341 gaaaccatgg tagataaagt cgcgcagata acgtccgctc tgagagcggc cccaggcata
     2401 ggcgtgggtg atccccccgc tggttcttcc tccgtcgcgg acggagagag gcgggcgcaa
     2461 cggattggct ttgccggcgc ggtctttcga ttcgaatcgc agaaacgata caaagtcacg
     2521 aaccacggcg aagcccagac ccaagacgag cggatttttt gcctggtaga tgaactcgta
     2581 aatatatcca ggcttgtagc cggcgcgcag ataaagatcg gtgccgctgg ctttgatgat
     2641 ttcattaccg ctgtgcgggg tttttgctgc tgcgccatat tcccattcat tcgccggaat
     2701 aggaattctt ttgccatagg atttttcgcg cacggtgagc attgcctttg atttgtcgcg
     2761 cgacgctgtc tcgtagctct tcacccgctc gtcgccgctg agcggctgtg atttaacgcc
     2821 tttctcgtcc acaacaattt ccgtgcgaac agtcgcgata atcggtttga ctgtgttggt
     2881 cgcgaccgga acgttgagca caagccagtt cttgagcggc atcaagtcgc cctgccagcc
     2941 gcaccagaca atcgtatagc cgtggcgcat gagaaaaccg ttgccggcat cttccaaggt
     3001 cgttggatcg ttggcttccg gcgcgtcgtt aaggaagccc aaaatgcgct tgctgccgcg
     3061 gttgggcgcg tcgaaaaaaa tcttgccatt gccgcgtgcc atatcgagcg gtttcaatat
     3121 gcaaaagttg ccggaatatt cgactcgtcc gcgttggttg cgcggcgctt tctcgacgtt
     3181 gacgataatc cggttccgcc gatgcttcgg atcgacttca ccgtagatca tgccaacgag
     3241 tttttcatag gcgccggtga tgccaaattc atgcccgcca gcgaagcgtt ctcttctttg
     3301 gacaacgaag cgattgatca taggatgagt tgagaatgga taattgaaaa ttgaccgtgg
     3361 ggaaatgcaa cgggctatta gtattcgttg aagattcgct gcaaatcctt tggatccttg
     3421 gttctcgtca ggccgagcat cagtaggatc cgcgctttgt gcgggatcag gctgtcgcct
     3481 tgtataaacg gatcgttcca atcaacggga atgcggccct ggccgccgcg gctggcgagc
     3541 acgaccggta ttcccgacgc ggcaattttc tctactccgc cgagttggtc ggcggagggg
     3601 cggccgttgt aggcataacc gctaattact aaacctcggg cgccggcggc cacgcaagct
     3661 tgcgccgcgg cgtcgtcacc gccgacgtat gcagcaatga catcgacgcg tggcagttcg
     3721 ccgatatcgc gaatgtcgaa ctcggaatgg aatgtgtggc gcctcgtggg agtagcataa
     3781 aaagacacct ggtcgtcctc gatgtggcca agcatgccgc gcgccgggga gtgaaagccg
     3841 ccggggcgct ggttggtctt cacgacttcg cgcgccgagt ggatctcctc gtgcaatgtt
     3901 accagcaccc ctttgccgcg ggcttccggc gtcaatgcca aacgaatcgc atcgagcagg
     3961 ttgcggtcgc cgtcgttgcc gacttcgtca tgtttgcgtt gcgaagcgac gatgaccagc
     4021 ggtttttccg tatgcacaca gaggtgaaga aagtaagcgg tttcttcagt ggtgaaggtg
     4081 ccgtgggtta cgatgatgcc gtcgacgcgg gcatcttccg ctgcggcggt ggcggcgcga
     4141 gcgatgtcga gccattgcgc caaacgcatc tggccgctac gcacccgcgt tgcttcaatg
     4201 acctcgaagt cggccatagt cttggcttgc ggtatgtcgc cgagcacttg gtcgatggcg
     4261 ataaggccgt gacccgtatt ggcgatggtt ccgccggtag agattattct aacgagtggc
     4321 atgagcttcc ttatcggcga ttcgacgcaa gaatgtctca tgcccatacc atcggacgat
     4381 tccggccgtc aaccgcgccg aaaacgaatg atatcttagc gcgcgtgacg agcaggaaag
     4441 ttgcgtcacc ggcgctaagt tgctaagaga gcttgcacat gggcggtttt acaaagtgaa
     4501 acgggagcga aacatgggga aacaagtgca cgctgtgctc gcgtccatac tgatcagttt
     4561 gagctgcgcg tcgctgctct gggcggccga agtcaaacgg ccgcccgaat gggacaagat
     4621 cgtggatgcc ggcaagaaag aagctaaggt ggtgatcgcg atccccccgt ccaacgagct
     4681 ccgcaaggaa ctcgaaaccg ttctcaaaca aaagttcgcc atagaggcgg agctgattgc
     4741 agcgccggga cctaagaatg ccagccggat cgccgccgaa aaaaaggctg gcgtgaatta
     4801 tttcgatgcg atcatttgcg gcaccggcac cgcggcaggg ctcacccatg acggcatgct
     4861 cgaaccaatg gaatcgttct gggtgcttcc cgaagttaag gacgccaagc aatggtgggg
     4921 cggccatatt tgggaggaca atgtcagcac gaacaaattt ttatattcct ttctcgccga
     4981 cgtggcgacc catgggattt ggtacaacac cagtttggcc aaagcggacg agctcaagtc
     5041 cttcgacgat tacttgaatc ccaagtggaa gagcaagatc ggctttagcg atccgcgcgt
     5101 gccgagttcc gggcaatcga tttggtcgtt tatgtgggag ctgaagggcg aagagttcct
     5161 caaaaaactt gttcagcagg aactcttctt gacgcgtgat ttgcggcagc tcgccgatgc
     5221 cctggcgaaa ggcaaagttg ccatggcgtt cggccttggg cgcagtcaga ccgatccgtt
     5281 tgtcgacgcg ggtctgccga tcaagccggt gccggcgcca aaagaaggat tgccggcgag
     5341 taattccttc ggcgtcattg gtattatcaa gaatccgccc aacccgaatg cgacgaaagt
     5401 attcgtcaat tggtttctca gccgcgaagg ccaggagtgg
//
LOCUS       DATLCH010000128         3279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3186386, whole genome shotgun sequence.
ACCESSION   DATLCH010000128 DATLCH010000000
VERSION     DATLCH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186386"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VMZ02_03775"
     CDS             complement(<1..243)
                     /locus_tag="VMZ02_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71081.1"
                     /translation="MIHLDYSIIYQIVLFVLLWLILDKLLFQPYLRSLAEREHKTTGA
                     QHDSVDLEHEGAKLKSQFDEKMAQGQAAGYAAKEAII"
     gene            complement(401..811)
                     /locus_tag="VMZ02_03780"
     CDS             complement(401..811)
                     /locus_tag="VMZ02_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HUR71082.1"
                     /translation="MSTSTAGLVGYLYKGSRVTGQLSFQGPARIDGIVDGEVNCQGIL
                     TVGEGAELKAKITGQIVVIRGKVEGNVTAKERIELLAPARLIGNIDAPRLIIAEGVVF
                     DGDCSMGVAKQKVGVAGSHGQSADKAAAASAPKL"
     gene            1376..2323
                     /locus_tag="VMZ02_03785"
     CDS             1376..2323
                     /locus_tag="VMZ02_03785"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71083.1"
                     /translation="MKLLIGLIAAWLAGSSASAGAEVVRGAYPSANVQFLPAFVALEK
                     GFYKREGLDAELISVRNAVTAVQALLGNQIHFIFSVGPQMPSIWEGSDILLLAQQIGR
                     PTFSMMVTQDIKSVADLKGKKIGVSFGGSTFAGTKALLELYKMNPDKDVQYVSIPGSQ
                     PKIAAMQQGIIQAALLAPPADYVAMKAGFKRLANLADLFKDTSFSGLAANGKTIKDNP
                     QFVKRMVRAIVHGVIHTRDYPEDAIQTMMKHWRMEREVSIDAYNLVKEALNPVPTEKG
                     VELMAQWQAVALNTKPKRPVREYMDLRFVNEVMAELTQK"
     gene            2339..2932
                     /locus_tag="VMZ02_03790"
     CDS             2339..2932
                     /locus_tag="VMZ02_03790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUR71084.1"
                     /translation="MKKILIVAVLCAVFGFGANASSQEKKGPLYATFKTSMGDIVVQL
                     FEDKAPKTVANFVGLASGTKEWTDTKTGEKVKKPLYNGTIFHRVIPGFMIQGGDPLGN
                     GTGGPGYRFEDEFHPDLRHTKGGILSMANAGPNTNGSQFFITLGPTLHLDNRHSVFGE
                     VIKGQEVVVAIGATPTSRDRPIKDVVIKEIVISRGSY"
BASE COUNT          812 a          917 c          831 g          719 t
ORIGIN      
        1 gataatcgct tctttagccg cgtaacctgc cgcttgcccc tgggccatct tttcgtcgaa
       61 ttgggacttt agcttggcgc cctcgtgttc aagatcgacc gaatcatgct gcgcacccgt
      121 ggtcttatgc tcgcgctctg ccaatgagcg caagtacggc tgaaaaagca gcttatcgag
      181 aatcagccac aggaggacga acaagacaat ttgataaatt atgctgtaat cgagatgaat
      241 catgggtcaa tgaccgctaa aagttaggct ttcaccttac tcgccgaacg cggaatggcg
      301 aaatacagca ctcaaataat aaaaaaccca cattctctac gaacgtagga tctaggattg
      361 ggaggtgacg gacagccgaa gattaatttc taaaatttat ctacagcttg ggcgcggaag
      421 ccgccgccgc tttatcagcg ctttgaccgt gcgaacctgc gactccaacc ttttgctttg
      481 ccactcccat ggagcagtcc ccgtcaaaca caacgccttc tgcaattatc aatcggggag
      541 catcgatgtt accgatcaag cgagccggcg ccagcaactc gatccgttct ttcgctgtga
      601 cgttgccctc aaccttgccg cgtatcacca cgatttggcc ggtaattttg gccttcaatt
      661 ccgcaccctc accaacggta aggatgccct gacaatttac ttcgccatcg acgattccat
      721 cgatacgcgc cggcccctga aaacttaact ggccggtaac gcggctcccc ttatatagat
      781 aaccgactag acccgccgtt gacgtcgaca caggggcggg cgcggcaggc gacggaacat
      841 cagccttatt agagatgggc tgaggtaaag gagccgcgac tgttacagtc ggctcggaca
      901 ccggcttcga tgcgggtgcg acgctttgcg ggtccgtttc ggtctttttt ccgcccgtgt
      961 ttttgtcaaa ccaagccatt ttatggttct cccgtgcttg ggcagagtct cgtataacat
     1021 ccgagaaact cattgtcaaa atgcattgga caaaaccaac ccggcatttc tgcgagttta
     1081 agtcgctatt agaacacgcc gtacttaact cgtaatatta cgaaagtatt accaactaaa
     1141 atctcaaaca acgtcgtaat cgacaaagtt gccaccgttc ttgaccgttc agagcacatt
     1201 gtttagctta ggatgattat ttgcatggca cagctgatcg aatcgataat cgcgcggaag
     1261 gcgatcgtca agttttcaac accatggtct atgaagaaac cgtctgcggc acaataccgc
     1321 tgacagtttt tcgcccgagc cttgttcaag cactctggtc ggaaacttca ctaatatgaa
     1381 acttctcatt ggcttaatcg cggcgtggct cgccggcagc agcgcaagcg ccggcgcgga
     1441 agttgtccgg ggtgcctatc cgtcggccaa cgtgcagttt ctcccggcat tcgtcgccct
     1501 cgagaagggc ttttataagc gcgagggttt ggacgccgaa ctgatctccg tgcgcaatgc
     1561 ggtcactgcg gtgcaggcac tgctcggcaa ccaaatccac ttcatctttt ctgtcggtcc
     1621 acagatgccg tcaatttggg aaggtagcga tatcttactg ctcgctcagc agatcggccg
     1681 gccaacgttc tccatgatgg ttacgcaaga tattaaatcc gtcgccgatt tgaagggaaa
     1741 aaagatcggc gtcagcttcg gcggctcgac cttcgctggc accaaggcct tattggaact
     1801 ttacaagatg aatcccgaca aggacgttca atacgtcagt attcccggca gccagccgaa
     1861 gatcgccgcc atgcagcagg ggatcattca ggcggcgctg ctggcaccgc cagccgacta
     1921 tgtagccatg aaagctggct tcaaacggct ggcgaatctc gccgatctgt tcaaagacac
     1981 gtcattcagc gggctcgccg ctaacggcaa aaccatcaag gacaacccgc aattcgtcaa
     2041 acgcatggtg cgcgcgatcg tgcacggcgt gattcacacg cgcgactacc ccgaagacgc
     2101 gattcaaacg atgatgaaac attggcgcat ggagcgcgag gtttctatcg acgcttacaa
     2161 tttggttaag gaagccctca atcccgtgcc aacagagaag ggcgtcgagc taatggctca
     2221 atggcaagcc gtggcgctca acaccaagcc caaacgcccg gtgcgggaat atatggacct
     2281 gcgcttcgtc aacgaagtca tggccgaact aactcagaaa taatttcagg aggcatgagt
     2341 gaaaaaaata cttattgtcg cagtattgtg cgctgtcttt gggttcgggg cgaatgccag
     2401 cagtcaggaa aaaaagggcc cgctttacgc cacttttaaa accagcatgg gcgacatcgt
     2461 cgttcagcta ttcgaagaca aggcacccaa gaccgtcgcc aactttgtcg gcttggcaag
     2521 cggcaccaaa gaatggacag ataccaagac cggcgagaaa gtgaaaaagc cgttgtataa
     2581 cggcacgatt tttcaccgcg tcattcccgg atttatgatc caaggcggcg atcccctggg
     2641 caacggcacc ggcggtcccg gttaccgttt cgaagatgaa ttccatcccg acctgcgtca
     2701 caccaagggc ggcattctgt cgatggctaa cgcaggtccg aataccaatg gcagccaatt
     2761 cttcattacc ttggggccga cgcttcacct ggacaatcgc cacagcgtgt tcggcgaagt
     2821 cattaaaggc caggaagtcg tcgtcgccat cggcgctacg ccgacctccc gcgaccggcc
     2881 gatcaaggac gtcgtgatta aagaaatcgt gattagtcgc gggagttact gagactggct
     2941 tatctgagcc aactcggatt acgcgcgcaa gaaccccgcg gatacgctcg ccatttgtcg
     3001 aaatacatta cagtatggtg agctacgaga gtcgctcgca accaacgcgc cggcctggca
     3061 cgaattccca catcgggcca tcaagcggtg ttagcggcgt cagagaatct caaagccgcc
     3121 aagtcagttc agctagtcga cgatcgcttt gtcaaacaat tagtcacggc acggaaattc
     3181 aaatgaggac atgacccgtg gttgttctgg gtcccccagc aacgtatgca tcattccaaa
     3241 tcatacggca cgcccaactg cgcgccaact tcgcgcaaa
//
LOCUS       DATLCH010000129         3256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2624993, whole genome shotgun sequence.
ACCESSION   DATLCH010000129 DATLCH010000000
VERSION     DATLCH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2624993"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VMZ02_03795"
     CDS             complement(<1..459)
                     /locus_tag="VMZ02_03795"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71085.1"
                     /translation="MQVPAIQGWGEAMMTSVAAALALLMAAVPKIMGFLLILLVGWFI
                     AGLIAKAVGAILRNVRFNDFARRSGLGDFVDQMGVQTDPAGVLAATVKWFVRLLVLIV
                     AFDALGLPAVSQVLNQLLLWMPNLVVALVVLVIAGLAANALHSLVRGATAK"
     gene            complement(619..2334)
                     /locus_tag="VMZ02_03800"
     CDS             complement(619..2334)
                     /locus_tag="VMZ02_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUR71086.1"
                     /translation="MRYLALACDYDGTLAHDGTISETTIAALENLLASGRKLILVTGR
                     ELDDLLKVCPRCDLFEWIVAENGCLLYRPATQEELFLYEAPPVEFAAALRALGVPVSI
                     GKVIVSTWVPHEVKVLEVIRQFGLELQVIFNKGAVMILPAGHNKASGLTAALDKMGLS
                     PHNVVGIGDAENDHAFLDLCGCGVAVANALPMLKDKADFVTQGENGSGVAELANEIVE
                     NDLAEREDLLVETHLLLAQRDDDGELRVAPLRANILLAGPSGGGKSTIIGGLLERLTE
                     SHYQVCVIDPEGDYDKVEGMATLGQPDRPPTVDEVLGLLKKAQDNIAINMVGIVFKDR
                     PEFFVSLLTRLQELRAKTGRPHFVIVDEAHHVLPESWQGDSLALPPSLKGLVFITVEP
                     KTLHRKVLAAVDMVLAIGAEPEKIFTDFATAADIPAPPLTGQSALERGEILVWQINPD
                     SQTFKARGIPGQAQRRRHLRKYAEGDLGPERSFYFRGRDGKLNLKAQNLIIFMQIADG
                     LDDKTWEYHLRRGDYSQWFKECIKDEALADEAIQIETNKKISSEESRKLIKAAITRRF
                     TLPAS"
     gene            2460..>3256
                     /locus_tag="VMZ02_03805"
     CDS             2460..>3256
                     /locus_tag="VMZ02_03805"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HUR71087.1"
                     /translation="MAEVIRLTDEYYIAAPSSTTHDSRVLKDGETFGVFDLYGDIRQT
                     GLGEQGIYHEGTRYLSHLLFRLGNNQPFLLSSTVKQDNQLFTVDLTNPDIYAQGQIVL
                     RRGDLHLFRSKFIWQASCYESLRIVNYGLAAVAFTFSFQFDADFADIFEVRGVKRAKR
                     GTRLPDQLDTDHLTFSYRGLDQAIRRTDLHFSPAPKILSASEALFDTTLQSKEEQTYT
                     LTYSFQSHTAHRPLRTPEMARRDAKQTLDALHTEECTIRTSNDEFNS"
BASE COUNT          764 a          924 c          854 g          714 t
ORIGIN      
        1 cttggcggtt gcgccgcgca ccaggctgtg gagagcgtta gccgccaaac cggcgatcac
       61 aagtaccacc aacgcgacta ctagattggg catccagagc aatagttggt tcaacacctg
      121 cgacactgcg ggcagtccca atgcgtcgaa ggcgacgatt agcacgagaa gtcggacaaa
      181 ccattttacg gtcgcggcga gtacgccggc cggatcagtt tgcacgccca tttggtcgac
      241 gaagtcgccc agaccggatc ggcgcgcaaa gtcgttaaaa cgcacattgc gtaaaatcgc
      301 gccgacggct ttggcgatca gcccggcaat gaaccagccg acgagcagga taagtaaaaa
      361 ccccatgatc ttcggcacag ccgccatcaa tagcgctaga gccgccgcga ccgacgtcat
      421 cattgcctcg ccccaccctt gaattgctgg aacttgcata aatcatctcc tctcgagatt
      481 tttttgcgtc acagttaaat gaagcaaaag gagaaccaaa ccgttttctg gctggaggca
      541 cgagtggcca gcgcgcggtc tcacatataa gacaatgcgc aacagctatg gtgcaaaatc
      601 agaacgtaga acgtgatgct aagaagcagg caaagtaaac cgtcgcgtaa tcgccgcctt
      661 aatgagtttc cgactttcct cggaagagat tttcttgttc gtttcaatct gtattgcctc
      721 gtcggcgagc gcttcgtcct tgatacattc tttgaaccac tgcgaataat cgccccggcg
      781 caaatggtat tcccacgtct tatcgtcgag accgtcggcg atctgcataa aaataatcaa
      841 gttctgcgcc ttgaggttga gctttccatc acgcccgcga aaatagaaac tgcgctccgg
      901 gccgaggtcg ccttcggcat acttgcgcaa gtgacggcgc cgctgagcct gacccggaat
      961 accgcgcgcc ttaaacgtct gcgagtccgg gttaatttgc caaaccagga tttcgccgcg
     1021 ctcgagcgcg gattggcccg tcagaggcgg cgccggaata tctgccgctg tcgcaaaatc
     1081 cgtgaaaatt ttctcgggct cagcgccaat cgcgagcacc atgtccactg ccgctaggac
     1141 cttgcgatgg agggttttgg gctcgacggt gatgaacact aaacctttta ggctcggcgg
     1201 tagggccagc gagtcgcctt gccaggattc cggcaacaca tgatgcgcct catcgacgat
     1261 cacgaagtgc ggccgtccgg ttttcgcgcg gagctcctga agacgcgtta ataaggatac
     1321 aaaaaattct ggtcgatctt taaaaacaat gccgaccata ttgatggcaa tattatcctg
     1381 cgcttttttc aataagccca aaacctcatc aaccgtcggc ggccgatccg gttgtcctaa
     1441 agtggccatg ccttcgactt tgtcataatc accttcggga tcgatgacgc agacctgata
     1501 atgagattcg gttagccgtt cgagcagacc gccgataatc gttgacttgc caccacccga
     1561 cggacccgcc agcagaatat tcgcgcgcag aggcgcaacg cggagttcgc cgtcgtcatc
     1621 tctctgggcc agcaacagat gagtttcgac gagcaagtct tcgcgctcgg cgagatcgtt
     1681 ttcgacgatt tcgttggcca gttctgcaac accactgcca ttttctcctt gcgtgacgaa
     1741 atcggctttg tctttgagca tcggcaaggc attggccacc gcgacaccac agccacagag
     1801 gtcgagaaaa gcgtggtcgt tctcggcgtc gccgatgccg acgacgttat gcggcgacaa
     1861 ccccatcttg tcgagagccg cggtcaaacc gctggcttta ttgtggcctg ccggcagaat
     1921 catcaccgcg cccttgttga agatgacttg caattcgaga ccaaactggc gaatgacctc
     1981 gaggactttc acttcgtgcg gcacccaggt tgagacaatc actttcccga tcgaaaccgg
     2041 cacaccgagc gcgcgcaatg ccgcggcaaa ttccaccggc ggcgcttcat agagaaacag
     2101 ttcttcctga gttgccggac gataaagtag gcagccgttt tcggcgacga tccattcgaa
     2161 taaatcacag cgtggacaga ctttcaggag atcgtcgagc tcgcgcccgg tgacgagtat
     2221 cagtttacgg cccgaggcca gtaaattctc gagcgcggca atggttgttt ccgatattgt
     2281 cccgtcgtgt gcgagggtac cgtcgtagtc gcatgcaaga gctagataac gcatgggggg
     2341 tcttcaacag caaaattgag accgtataat aaaaatagtg agcgcgaccg gttccggcca
     2401 gagtttgccc cgctcacgca accacttaac ccattcactt aaaaatcata aggaaaatta
     2461 tggctgaagt gatccgtttg accgatgaat attatatcgc cgcgccttcg agcacgaccc
     2521 acgattcgcg cgtgttgaag gatggcgaga ctttcggggt gttcgatctc tatggagata
     2581 ttcgtcagac cggtctcggc gaacaaggga tttatcacga aggaacacgt tacctctcgc
     2641 atctgctgtt ccgcctcggt aacaaccagc cgtttctact gagctcaacg gtgaagcagg
     2701 ataatcagct tttcactgtt gatctcacta atccggacat ttacgcgcag gggcaaatcg
     2761 tcttgcgtcg cggtgatttg catctgtttc gctcgaagtt catctggcaa gcgagttgtt
     2821 acgagtcgct gcgcatcgtc aactacggtc tggcggctgt tgcattcacc ttttcgtttc
     2881 agttcgatgc ggattttgcc gacatattcg aagtgcgcgg cgtcaagcgg gcgaagagag
     2941 gaacgcgttt acccgaccaa ctcgacaccg atcatctgac tttttcctat cgtggtctcg
     3001 accaggcgat ccgccgtacc gacctgcatt tttcgccggc accgaaaatt ctgagcgctt
     3061 cggaagcgct gttcgacacg actttgcaaa gcaaggaaga acagacctat acgctcacct
     3121 attcgttcca gagccacacc gcgcaccggc cgcttcgaac gcctgagatg gcgcgcaggg
     3181 atgccaaaca gacgctcgac gcgctgcaca ccgaagaatg cacgatccgc acctccaatg
     3241 acgagttcaa tagctg
//
LOCUS       DATLCH010000130         3523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8392264, whole genome shotgun sequence.
ACCESSION   DATLCH010000130 DATLCH010000000
VERSION     DATLCH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8392264"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VMZ02_03810"
     CDS             <1..1122
                     /locus_tag="VMZ02_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71088.1"
                     /translation="EASTHITRAGARLDMIEATQHDRFAFEDYALLRDVGMTTVRDAL
                     RWHLIDRGGQYDFSSFTPMLEAAQEAGVQVIWDICHYGLPDGLDIFAPEFVNRFARFC
                     GAVAGLVKRYSDGVPFYAPINEISFFAWAAGHVGFMHPCAVDRGGEVKRQLIRAAIAG
                     IEAIWAVDSRARIAQIDPIMHVVTPRSHPELADAAAGQNASQFEAWDMLCGLASPELG
                     GDPRYLDIIGVNYYHANQWEYPGDQPENRLRWEDSPRDSRWVPFSQLLIDFYARYRRP
                     MFIAETGHFGVGRALWAKETAAEVMIARRRGVPIEGICFYPIIDRTDWEDASHWHNCG
                     LWDLHRDDQGRLQRVPCAEFAAELSSLMCASGTQQLTRT"
     gene            complement(1159..2100)
                     /locus_tag="VMZ02_03815"
     CDS             complement(1159..2100)
                     /locus_tag="VMZ02_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUR71089.1"
                     /translation="MKVKPKRKPIKWPDGARIAITPCVAFETWPEDLGGPNTLQQENR
                     RSFPRNAETKRNLAILTDREFGERVGIYRMLEVFESEKIRTSFFPTGITVENYPEIFQ
                     DAAEYGHEIGTETWIHDYSYMKKRPQEKKDLLKTAAAVKKVVGKAPVGYLSTGVAPSF
                     STPEVILECGYTYWMDPQHQETPYTLKVGKKELTVLSYFQDLNDYSSYQRAGRTPRDL
                     LQMWIDTFDCLYEEGASDPKFMMWGNHPFVGGRPFRAFLLREFIRYAKKHEKVWFATA
                     GEVAQWYRENYHDAQVEEWPNFYHAGRRGKISDLAKL"
     gene            complement(2313..3347)
                     /locus_tag="VMZ02_03820"
     CDS             complement(2313..3347)
                     /locus_tag="VMZ02_03820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71090.1"
                     /translation="MGVTKFGSHYLPTYVPDLDGSVSEFYQKMFGQMEEMDRLGYDHI
                     WVTEHHFAHYGGDLPHPPTFMSAIARTTKQIRLGIAISVLPLHNPIDLAESYAMVDVI
                     SHGRLDFGVGKGSEAHEYKKYRVDQKEATGRMYEGVEVMLQAWSDKPVSFKGEFFNYD
                     NVPVFPKPVQRPHPRFWVGCARSEDSFRWAGEKGFNLMTLPYLYREAGALPGFIKIYR
                     DNLTKAGHDAATKDILGKFHIYVSTSLDKAIEEAAPYLERYVDVHHAADTDRKEVGLL
                     VQRDAKTQIKEGFVLAGDPERVADTIRKWAEPNDLTTISGTFHFGGMPQEMALKNIRL
                     FAEKVMPQFK"
BASE COUNT          735 a         1000 c          949 g          839 t
ORIGIN      
        1 gaagcgtcta cacatataac ccgcgccggt gcccggctcg atatgatcga ggcgacgcag
       61 cacgatcgct ttgcctttga agattatgcg ctgttgcgcg acgtcggcat gacgacagtg
      121 cgggacgccc tgcgctggca cctgatcgat cgcggcggtc aatacgactt ttcttctttc
      181 acgcccatgc tcgaagcggc gcaagaagcc ggcgtgcaag ttatttggga tatttgccat
      241 tacggcttgc cggacgggct cgacattttt gccccggagt tcgtcaaccg cttcgcgcgc
      301 ttttgtggcg ccgtcgctgg attggtaaaa cgctacagcg atggtgtgcc gttctatgcg
      361 ccgatcaacg agatttcgtt ttttgcctgg gccgccggtc acgtgggctt tatgcatccc
      421 tgtgctgtag accgaggcgg agaagtgaaa cgacagctca tccgagccgc catcgccgga
      481 atcgaagcga tctgggctgt cgattcacgc gcgcgcatcg ctcagataga tcccatcatg
      541 catgtcgtga cgccgcgctc tcatccagag ctcgccgatg cggccgccgg acaaaatgcc
      601 tctcaatttg aggcttggga catgctctgc ggcctggcga gcccggaatt gggcggcgac
      661 ccacgctatt tggatatcat cggggtcaat tactaccatg ccaatcaatg ggagtatccg
      721 ggcgaccaac ccgaaaaccg gctacgctgg gaggactcgc cgcgcgacag ccgctgggtg
      781 cctttttccc aattgctcat agatttctac gctcgctacc ggcgccccat gtttatcgcc
      841 gagaccggac acttcggtgt tggccgcgcc ctttgggcca aagaaacagc cgcggaagtg
      901 atgatcgcgc ggcgccgtgg cgttccgata gagggtattt gtttctaccc gatcatcgat
      961 cgaaccgatt gggaggacgc cagccattgg cacaactgcg gcctttggga ccttcatcga
     1021 gacgaccagg gtcggttgca acgagtcccg tgtgcggaat tcgcggccga gttatcttcg
     1081 ctgatgtgcg ccagcggcac ccagcagcta acgcgtacgt aactgccccg ccaaacgcgt
     1141 atcgttgatt cttgattcct acagctttgc caaatcgctg atcttgccgc gccggccggc
     1201 atgataaaaa ttcggccact cctcgacttg ggcgtcgtga taattttccc ggtaccattg
     1261 cgccacttcg ccggccgtgg caaaccaaac cttttcgtgt ttcttggcgt agcgaataaa
     1321 ctcgcgcaac agaaacgcgc ggaacggccg gccgccgaca aacgggtgat ttccccacat
     1381 catgaacttt ggatcgctgg cgccttcttc atacagacaa tcgaaagtat cgatccacat
     1441 ctgcaagaga tcgcgcggcg ttctgccggc gcgctgatag ctggaataat cgttcaaatc
     1501 ctggaagtag gacagcaccg tcaattcttt ttttccgacc tttagggtgt aaggcgtttc
     1561 ttgatgctgc gggtccatcc aatacgtgta gccgcattcc aggatcacct caggcgtcga
     1621 gaagctcggg gctactccgg tcgaaagata gcccaccggc gccttgccaa cgactttctt
     1681 gacggcggca gccgtcttga gcaaatcctt tttttcttgc ggccgttttt tcatatagct
     1741 gtaatcgtga atccaggttt ccgtaccgat ctcgtggcca tactcggccg cgtcctggaa
     1801 aatttccggg tagttttcca cagtgatccc ggtggggaaa aaactcgtgc gaatcttttc
     1861 actttcgaat acttcgagca tgcgatagat gccgacgcgc tcgccgaact cgcgatcagt
     1921 gaggatcgcc aggtttctct tggtctcggc attacgcgga aagctgcggc gattttcttg
     1981 ctgtaaggtg ttgggtccgc ccaaatcttc cggccaggtt tcaaaggcga cacacggcgt
     2041 gattgcgata cgcgcgccgt cgggccactt gatcggcttg cgtttaggtt taactttcat
     2101 actgcctccc tggatattca gaaaattttt ctactgatgc catagttctg gcgcattgcc
     2161 aatcatgcat ggctaaaaaa aaggcgggtt ttaaacccgc ccctacagga atccaaattc
     2221 gtttatatgt tctttgcgtt ctttgcggct aaaaatccga ttatgcgacc tttgtgctct
     2281 atgtggtcaa atttcccaag ccgaatcttc ccttacttga actgcggcat aactttttcg
     2341 gcgaacaggc ggatgttttt cagcgccatc tcctgcggca tgccgccgaa gtgaaaggtt
     2401 cccgagatcg tcgtcaagtc gttaggctcg gcccatttgc ggatcgtgtc ggcgacgcgc
     2461 tcgggatcac ccgccaagac aaacccttct ttgatctggg tcttggcgtc gcgttgcact
     2521 agcaagccga cttctttgcg atcggtatcg gcggcgtgat gcacgtcgac gtagcgctcc
     2581 agatacggcg cggcctcttc aatcgccttg tccaagctgg tcgacacata aatatgaaat
     2641 ttgccgagga tatctttggt agcagcgtca tgccctgcct tggtcaaatt gtcgcgatag
     2701 attttaataa agcccggcaa tgcgcccgct tcccgataga gatacggcag cgtcatcaag
     2761 ttaaagcctt tttcaccggc ccagcgaaac gagtcttcgc tgcgcgcgca gccgacccaa
     2821 aagcgcggat gcggccgctg taccggcttg ggaaacaccg gcacgttgtc ataattgaag
     2881 aactcgcctt tgaagctcac cggcttatcg gaccaggctt gcagcatgac ctctacgcct
     2941 tcgtacatgc gcccggtcgc ttccttctga tcgacacgat atttcttgta ctcgtgcgcc
     3001 tcgctgcctt tgccgacgcc gaaatccagc cggccatggg aaatcacatc gaccatggca
     3061 taggactcgg cgagatcgat gggattgtgc agcggcaaga cgctgatggc gatgcccaga
     3121 cggatttgct tggtggtacg ggcgatcgcc gacatgaatg tcggcggatg gggcagatca
     3181 ccgccgtagt gtgcaaagtg atgctcggtg acccagatat gatcgtaacc aagccggtcc
     3241 atctcttcca tctgaccgaa cattttctgg taaaattcgc tcacggatcc gtcgagatcc
     3301 ggcacgtaag tcggtagata atgtgatcca aacttggtca cgcccatggt ttgctaaacc
     3361 tccaggaaat ttgactcaac atataagtag ctcgccggga agaagtcaac gccgccacac
     3421 tggatcggtc aacagcgtga gatttacatc gcgtgaacat tcgcatagta aagttagata
     3481 gcgctctatc gaaataggag gcgccacatg attcacacgc gca
//
LOCUS       DATLCH010000131         6583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9329498, whole genome shotgun sequence.
ACCESSION   DATLCH010000131 DATLCH010000000
VERSION     DATLCH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9329498"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VMZ02_03825"
     CDS             complement(<1..267)
                     /locus_tag="VMZ02_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HUR71091.1"
                     /translation="MRRKSKKIRQIVWALLLASSILSTNSESTQAAESNSAEPIVDAL
                     QKTYDATVDFVADFRQETEVKTLGRSLKASGKVSYKRPGKMFWNY"
     gene            complement(242..2509)
                     /locus_tag="VMZ02_03830"
     CDS             complement(242..2509)
                     /locus_tag="VMZ02_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HUR71092.1"
                     /translation="MPAAIEKSRLNREIWGVVIALTALLIAISLVSYSVNDRSFNTPS
                     GAVTTHNWGGFLGAFLADLLLQGLGLSSYLVPIFLVCGAVQMFRASYEGISLARASAY
                     AVLLVSVGILLSVLIDSESARDAGGIVGGFLEESILIPLFGRLSSVLIACFMLLLSIM
                     MLTQYSLLDLIGHTRKNFALFKKSLVPAINDRVREIKEKNDKRKGENTKKDKKDYTPP
                     AILTKSDAPDDLVIKKSARKPAAPAPEQFDLPEVGEGYKLPPLELLDPPEHEQISIDK
                     ETLHANSLILQKKLEDFGVEGEVVAVRPGPVITMYEFKPAPGVKVRRIVMLADDLAMA
                     LRAVSVRILAPIPGESVVGIEIPNPRRETVYLREVIESESYRAADSKITLALGKDIGG
                     VAFSTDLARMPHLLVAGATGTGKSVSINAMILSLLFKASPQDVKFIMVDPKMLELTVY
                     EDIPHQLVPVVTDPKKAAAALFWAMDEMDRRYRLMREKAVRNIDGYNRAVEKETGNKK
                     DLIELTEAEPMEETPAIGGRLSKDAPLVHGHLPRIVIIIDELADLMMTVGRDIEEYIT
                     RLAQKARAAGIHLILATQRPSVDVITGLIKANFPARISFQVTSRIDSRTILDSMGGEK
                     LLGNGDMLFLPPGTARLTRIHGAFVSDREVRKVMNFIKQQGRPNYRTDILEAKKETEA
                     AAAADEDYDEMYDQAVAIVTETQQASISMIQRRLRVGYNRAARMIEVMERDRVIGPAD
                     GAKPREVYAQKIEED"
     gene            complement(2522..4177)
                     /locus_tag="VMZ02_03835"
     CDS             complement(2522..4177)
                     /locus_tag="VMZ02_03835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HUR71093.1"
                     /translation="METLKIIPLGGLGEFGLNMMLIEYGADAIAVDCGLMFPDAHLLG
                     IDLVIPDVSYLLEQKTKLKAIVLTHAHEDHVGALPFVLKHFDVPIYGTKLTLGLLANK
                     LKEHDLEESTEIHEITAGAPWQIGPFSVEGIRVTHSLMDCLALAIETPVGTVIHTGDF
                     KIDNTPMDGEMFDFQRFAAYGEKGVLLLMSDSTNVERRGHTPSEREVGTNLEQIIVKS
                     TGKVLVSTFSSSIPRIQQVVEIAERCDRRVVLSGRSMIRNCEVAAELGYLRLPRSFLT
                     DSDRWQGLAPDRLMFLTTGSQGEPMSVLHRVALDDHKTLKVERGDTVILSSKFIPGNE
                     KVISNLINHLYRRGAEVHYEKVSEIHVSGHASQEELKIMLQLTRPRYFVPIHGEYRHL
                     VRHRRLAQEVGIPEDNCFLLDDGDVLELTSRGAEKVQPIQVGRVFVDGKGIGDVGDMV
                     IRDRRHLSEDGMVLAVMAIHQQSGELVAGPDLISRGFMLAEESDEVLESAKQAVLAAL
                     AGMNRETRTDPEELKEEVRRVLRRFFRKKLDRRPVVLPYIIET"
     gene            complement(4184..4954)
                     /locus_tag="VMZ02_03840"
     CDS             complement(4184..4954)
                     /locus_tag="VMZ02_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUR71094.1"
                     /translation="MFYSVKLVIVIAATVVLSLLTILGGPFDANGKRVYRISQLWTWI
                     IVKFSGIRLTVEGLDRLDPKRQYLFVVNHQSNFDIPVLVQSLPQFQLRWIAKKELLWI
                     PFFGWAMWAAKHITVDRADSLDALKSLKLAKQRIAAGISVVVFPEGTRSTDGKLLPFK
                     RGGFLLALKTKTPIVPVTINGTGKLLARGEWRVQPGAINVTISAPIMAHEFRPGGLRG
                     LSAKVQEIIAANLQTNEPFDRNQESLTRASITAPGSLV"
     gene            5145..5582
                     /locus_tag="VMZ02_03845"
     CDS             5145..5582
                     /locus_tag="VMZ02_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HUR71095.1"
                     /translation="MSVNKVILVGNLGKDPEVRYTQTGSAVANFSLATSEQWNDRDGK
                     KQERTEWHNIVVWGKQAEHCGQYLSKGRQVFIEGSIRTRSYDDKTGNKRYITEIVAQR
                     IQFLGGGGGTRLPQQGMESGSVDDMAMGGMGGGQPPLDDDIPF"
     gene            5832..>6583
                     /locus_tag="VMZ02_03850"
     CDS             5832..>6583
                     /locus_tag="VMZ02_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HUR71096.1"
                     /translation="MIPYTSKLTFGPETADWQERLNTERMRNQRVARAQAMMKKHGVA
                     ALLEANHHNIRYLTALRGFIYPMCRYVLFFAEQDPVMYEHDGYFHQMPDQCPWIKEWR
                     PAHSWLSGAPGPGACADEAKAFAAEIKAELEKRGLLGEKLALGSFDGNAREALVNAGV
                     KNIVDSRNLMLEARMIKNQDEINCLKTCASMVDGVWYRVWENLKPGIRDTDLMRIASG
                     AGWEMGLEGSVPGGWRTGPTTFDRGPHATSRV"
BASE COUNT         1460 a         1781 c         1842 g         1500 t
ORIGIN      
        1 gtagttccag aacattttac cgggccgttt gtaggaaact ttgccggacg ccttgagact
       61 gcggccaagc gttttgactt cggtttcttg gcggaagtcg gcgacgaaat cgaccgtcgc
      121 gtcgtaggtt ttttgcagcg catcgacgat tggttcagcg ctgtttgatt ccgcagcttg
      181 ggttgattcg ctgttcgtcg acaggatgct cgatgcaagc agcagcgccc aaacgatttg
      241 cctaatcttc ttcgattttc tgcgcataaa cttctctcgg tttggcacca tcagccggac
      301 cgatgacgcg gtcgcgttcc atgacttcga tcatccgcgc agcgcggttg tagcctacac
      361 gcaatctccg ttgaatcata gagatcgatg cttgttgggt ctcggtgacg atggcgaccg
      421 cttgatcgta catctcatcg taatcttcat ccgcagctgc cgcggcttcg gtctcttttt
      481 tcgcttcgag tatgtcggtc cggtagttag gccgaccctg ctgcttgata aaattcatta
      541 ctttgcgcac ttcgcgatcc gagacgaagg cgccgtgaat acgcgtcagc cgcgccgtgc
      601 ccggaggtaa aaagagcata tcgccattgc cgagaagctt ctcaccgccc atggagtcga
      661 gaatcgtccg cgaatcaatc cgcgatgtga cttggaagga aatgcgcgcc gggaaattag
      721 ctttgatcag accggtgatt acatcgaccg aagggcgttg ggtggcgagg attagatgta
      781 tgcccgcggc acgagctttt tgggccaaac gggtaatata ttcttcgatg tccctgccga
      841 ccgtcatcat gagatcggcc aattcgtcga tgatgataac gatgcgcggc agatggccgt
      901 ggaccaaagg tgcgtctttc gatagcctgc cgccaatagc cggagtttct tccatcggtt
      961 ctgcttcggt caactcgatc aagtcctttt tgttgccggt ctctttttcg acggcgcggt
     1021 tgtagccgtc gatattccgt accgctttct ccctcattag acgatagcgg cgatccatct
     1081 catccatcgc ccagaagagc gcggcagcgg ctttcttcgg atcggtgacc actggcacta
     1141 gttggtgcgg gatgtcttcg tagacggtca actcaagcat cttcgggtcg accatgatga
     1201 acttgacgtc ctgaggactg gccttgaaca agaggctcag gatcatcgcg ttgatcgata
     1261 cagatttgcc ggtgccggtg gcgccggcga ccaagaggtg tggcattctg gccagatctg
     1321 tggaaaaggc cacgccacca atgtctttgc cgagcgccag agtgattttc gagtcggcgg
     1381 cgcgatagga ttcactttcg attacttcgc gcaaataaac cgtctcgcgc cgtggattgg
     1441 gaatctcgat gccgacgacg gattcgccgg ggatcggcgc caagatgcgc acgctgacgg
     1501 cgcgcagcgc catcgccaaa tcgtcggcca gcatgacgat gcgccggact ttgacgccag
     1561 gcgccggctt gaactcgtac atcgtgataa ccggccccgg gcgcaccgcg acgacttcgc
     1621 cctcgacgcc gaagtcttcg agttttttct gcaggatcaa agagttggcg tgcagggtct
     1681 ccttgtcgat actgatctgc tcatgctccg gcggatcgag cagctccagg ggcggcaatt
     1741 tgtatccttc gccgacctct ggtaggtcaa actgttccgg cgctggtgcc gccggctttc
     1801 tggcggactt tttaatcact aaatcgtcgg gcgcatcgct cttggtgaga atcgccggcg
     1861 gcgtgtagtc ttttttatct ttcttagtgt tttctccttt gcgtttgtcg ttcttttctt
     1921 tgatttccct gacgcgatcg ttaattgccg gcactaatga ttttttaaac agggcaaaat
     1981 tctttcttgt atgaccgatc aaatcgagga gcgaatactg ggttagcatc atgatggaca
     2041 agagcagcat gaagcaggcg atcaatactg aactcaaacg gccgaacagc ggaatcaaaa
     2101 tactttcttc gagaaagccg ccgacaatgc cgccggcatc gcgggcactt tcactgtcga
     2161 tcaggacgct gagcaagatg cctacgctaa ccaggagaac cgcgtaagcc gacgcgcgag
     2221 ccagagaaat accctcgtag ctggcgcgaa acatctgcac cgcgccgcac accaggaata
     2281 tcgggacgag gtaggacgag agtcctagac cttgtagaag caggtcagcg aggaacgcgc
     2341 caagaaagcc gccccagttg tgagtggtaa ccgcacccga cggtgtattg aacgagcggt
     2401 cattgacgct gtaggaaacc aagctgatgg ctatgagtaa tgccgtgagc gcgatcacta
     2461 cgccccagat ttcacggttg agtcgacttt tttcgattgc agctggcata attggctttc
     2521 cttaggtttc tatgatgtag ggcagcacga cgggacggcg atcgagtttt ttcctaaaaa
     2581 acctccgtaa aacacggcgc acttcctctt tgagttcttc cggatcggtg cgcgtctcgc
     2641 ggttcatgcc ggcgagcgcg gcaagcacgg cctgttttgc gctctccagg acctcgtcgc
     2701 tttcctcggc cagcataaaa ccacgggaaa tcagatcggg cccggcgacg agttcgccgg
     2761 attgctgatg gatcgccatg accgccaaga ccatgccgtc ttcggacaga tgacgccggt
     2821 cgcgaatgac catgtcaccg acgtctccga tgccttttcc gtcgacgaaa acgcggccaa
     2881 cttggattgg ctggactttc tcagcgccgc gcgaagtcag ctcgaggacg tcgccatcgt
     2941 cgagtagaaa acagttgtct tccgggatgc cgacctcctg ggcaagccgg cggtgacgca
     3001 ccaaatgacg gtattcaccg tggatcggca caaagtagcg aggtcgggtg agctgcaaca
     3061 taatcttcag ctcttcctga ctggcgtggc cggaaacgtg gatttcggaa actttttcgt
     3121 aatggacctc ggcgccgcgg cggtagagat gattgatcag gttcgagatt accttttcgt
     3181 tgccgggaat aaacttggac gagagaatta cggtgtcgcc acgttcgact ttgagcgtct
     3241 tgtgatcatc caatgcgaca cgatgcaaga ccgacatggg ctcgccttgg ctgccggtgg
     3301 tgagaaacat cagacgatcg ggcgccagac cttgccagcg gtcgctgtca gtcaggaaac
     3361 tgcgcggtaa gcgcaaatac ccgagttcgg cagcgacctc acagttgcga atcatgctgc
     3421 gcccgctgag cacgacacgc cgatcgcagc gctcggcgat ctcaaccact tgctgaatac
     3481 gcgggatgct cgaggaaaac gtcgaaacca gtactttgcc ggtgctctta actatgatct
     3541 gttccagatt ggttccgact tcacgctcgg aaggcgtgtg gccgcgccgt tcgacgttgg
     3601 tcgaatccga catcaataac aacacgccct tctcgccgta ggcagcaaaa cgctggaagt
     3661 cgaacatctc gccatccatc ggggtattgt cgatcttgaa atcgccggta tggatcaccg
     3721 tgccaaccgg cgtctcgata gcgagcgcga ggcagtccat caagctgtga gtgacgcgga
     3781 taccttcgac actgaaggga ccgatttgcc acggcgcgcc ggcggtaatc tcgtggatct
     3841 ccgtgctctc ttcgagatcg tgctctttca atttgttggc caataagccg agcgtcagct
     3901 tggtgccgta gatcggaaca tcaaaatgct tcaagacaaa gggcagcgcg ccgacatgat
     3961 cctcgtgagc atgggtgagc acgatcgcct ttagcttggt cttctgttcc aacaaataac
     4021 tgacgtccgg gatcactaaa tcgatgccta acaaatgcgc gtcggggaac atgaggccgc
     4081 agtccaccgc gatggcgtcg gcgccatact cgatcagcat catgttcaag ccgaattcgc
     4141 cgaggccgcc cagcgggatg atcttcaagg tttccataaa tagttacacg agacttcctg
     4201 gcgccgtgat agacgctctc gtcaagcttt cctggtttct gtcaaatggc tcgttggttt
     4261 gcagattagc tgcgatgatt tcttgaacct ttgcgctcaa accgcgcaat ccacctgggc
     4321 gaaattcgtg cgccatgatc ggcgcactta tcgttacatt gatcgcgccg ggttggacgc
     4381 gccattcacc tctggcaagc agtttacccg tgccattgat ggtcaccggc acaatgggtg
     4441 ttttggtttt cagcgccagg agaaagccgc cgcgtttgaa cggcaacaac ttgccgtctg
     4501 tactacgggt gccttcggga aataccacca ccgaaattcc cgccgcgatg cgctgcttgg
     4561 cgagcttcag acttttcagc gcatccagcg aatccgcccg gtccaccgtg atatgtttgg
     4621 ccgcccacat agcccaacca aagaaaggta tccagagaag ctctttcttg gcgatccagc
     4681 gtagctgaaa ctgtggcaat gattgaacca gtacgggaat gtcgaagttg ctttggtgat
     4741 tcacaacaaa aagatactgg cgcttagggt ctaggcgatc caaaccttct acggttaacc
     4801 tgatgccgct gaatttgaca attatccaag tccacaactg actgatgcga tagactcgtt
     4861 tgccgttggc atcgaatggg ccgcctaaaa tcgttagcaa cgaaagcacc accgtagccg
     4921 cgataactat cactaatttg actgagtaaa acatcaaggc aaggctaaat ttggacaatt
     4981 gaacccattg gaaattaata ccaattgtag agaaggcttg gataaaaatc aatgtaagga
     5041 agtttcccga agttgagcta aatcgagttg actctccgga tagccagggt taagttggga
     5101 gcacttttcc agcgcggttt cttttcaaaa gttctggagg gcgcatgtcc gtcaacaaag
     5161 ttattctggt cggcaatctt ggtaaggatc ccgaggtgcg ttacacccaa acaggcagcg
     5221 ctgtggcgaa cttctctcta gccacttcgg aacagtggaa cgaccgcgac ggcaagaaac
     5281 aagaacgcac cgagtggcac aacatcgtgg tctggggcaa gcaagccgag cattgcggtc
     5341 aatatttgtc caaaggtcgg caagttttca tcgagggaag cattcgcacg cggagctatg
     5401 acgataagac tggcaacaaa cgatacatca ccgaaatcgt ggcccagcgg attcaattcc
     5461 tcggcggtgg tggtggaact cgtttgccgc agcaaggtat ggaatccggg agtgtcgatg
     5521 atatggccat gggcggcatg ggcgggggcc agccgccgtt ggacgacgat attccattct
     5581 aaattttata tacgcgcaga gatcgaagcg cttctctcgg tccaagagca atctttaaat
     5641 gcattaactg ctgatcatgc ccattaacgg ctcctcaccg ttataaaggt aaagccgatc
     5701 gcccgccaat gtccgcaacc tgcaaattgt aacccgacgg gtcacggaaa agcgctgctc
     5761 ggttcttttg caatctcatt tggcatccac gtgcgagcag cgcgagcatt ccctatattc
     5821 tggagcgcga catgattcca tacacatcga agttgacgtt cggtccggaa acagccgatt
     5881 ggcaggagcg tctcaacacc gaacgcatgc gcaatcagcg tgtcgcgcgg gcgcaggcga
     5941 tgatgaagaa gcatggcgtc gccgcgctcc tcgaagcgaa ccatcacaac attagatatc
     6001 taaccgcgct gcgcggattc atctatccaa tgtgtcgtta tgtgctgttt ttcgccgagc
     6061 aggatccggt gatgtacgag cacgacggct attttcatca aatgccggat cagtgtccgt
     6121 ggataaaaga gtggcgcccg gctcactcgt ggttgagtgg tgcccctggc ccgggtgctt
     6181 gcgccgatga ggcgaaagcc tttgcggcgg aaatcaaagc cgagttggaa aagcgcggcc
     6241 tgctcggcga aaagctcgcg ctcggcagtt tcgacggcaa cgctcgcgaa gcgctggtca
     6301 acgccggcgt gaaaaacatc gtcgactcgc gcaatctcat gctcgaagcg cgcatgatca
     6361 aaaatcagga tgaaattaac tgtctcaaga cctgcgcgtc gatggtcgac ggagtctggt
     6421 atcgggtctg ggaaaatttg aagccgggga ttcgcgacac cgatctgatg cggatcgctt
     6481 ccggcgccgg ctgggagatg gggctcgaag gctccgtgcc aggcggttgg cgcaccgggc
     6541 cgaccacctt cgaccgcggt ccgcacgcca ccagccgggt gat
//
LOCUS       DATLCH010000132         8510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4861533, whole genome shotgun sequence.
ACCESSION   DATLCH010000132 DATLCH010000000
VERSION     DATLCH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4861533"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VMZ02_03855"
     CDS             <1..1019
                     /locus_tag="VMZ02_03855"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HUR71097.1"
                     /translation="GKSLWRRQPIAAYIQPPDVNSDTQEKLKTVLAVREYARDVLKLN
                     VGGSYASYSYVDRPDLTYVVIAAPQTELRPYTWWFLIVGRVPYKGYFSRQDAEGEIER
                     LKAKGYDTNLRTSAAFSTLGWFDDPLLSHLLKYDKLGLSEIVFHELFHNTLYVSGQGA
                     FNESVATFIGHRAAIDFFRAKFGADSAEHKRAIEWWSEEREFGAYIGEIARDLTELYN
                     SAIPREVKLRLREEVFTRSKAEWARRIADRPMHRFRGFSQQALNNAVLMHYVVYLKDL
                     DLFESLYESAGYNLPKTIELLQQAAGKGGDPFDAVRRLVNRFNRENRQEHDERPDVKT
                     PGLS"
     gene            complement(1014..1874)
                     /locus_tag="VMZ02_03860"
     CDS             complement(1014..1874)
                     /locus_tag="VMZ02_03860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HUR71098.1"
                     /translation="MRIGVVFPQTEIGADPYYIKDYGQTAEALGFSHILAYDHVVGAN
                     PASRADWRAPYSHLDMFHEPLVLFSYLAGVTERLEFCTGIIILPQRQTVLVAKQTAGL
                     DVLSGGRLRFGVGIGWNPVEYEALGEDFKNRGARCEEQIEVLRQLWTNPTVTFQGRWH
                     KITDAGINPLPVQRPIPIWFGGSDERALRRLARLGDGWFPLLGPDEKCQAMIDKIHSY
                     ARDAGRRPQAIGIEGRISYGQGAPDAWRKELLAWQNLGATHVSFNTMKAGLNSPSAHI
                     EAIRQFAGLL"
     gene            2036..2917
                     /gene="htpX"
                     /locus_tag="VMZ02_03865"
     CDS             2036..2917
                     /gene="htpX"
                     /locus_tag="VMZ02_03865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF002826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HUR71099.1"
                     /translation="MNGLRTTILLALLTALVVWIGQMVGGPQGAVMALIFAGVMNFFS
                     YWFSDKIVLKMYRAQEISAADDPELYGIVQDLATRGGLPMPKVYIIPEDTPNAFATGR
                     NPEHAAVAVTAGIRRILNKRELAGVLAHELTHVKNRDILVSSIAATLAGAISYLAHMA
                     QWGMIFGGGRSRDDEGGGSNIFGLLFMMIVAPIAAMLIQMAVSRSREYMADTGGAKIS
                     GDPLALASALRKLHMGAQNIPLQVSDATATSTAHMFIVNPLSGAGLANLFSTHPAMEE
                     RIARLEAMAKDMSYLHA"
     gene            complement(2922..3596)
                     /locus_tag="VMZ02_03870"
     CDS             complement(2922..3596)
                     /locus_tag="VMZ02_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HUR71100.1"
                     /translation="MNLIHTVYQPAGPGPHPTILTLHGRGANAFDLLGLAPYIGGGKF
                     LIISPQAPIETPIGPQAVGYAWYGASAGGVPDIEGMLTSLSVLQLFVDDCFKRYDLDP
                     TRLAVLGFSQGGVMAYSLALTHPQRFAGLAALSTWLPRELTPRLAISAAVQSLPTLIQ
                     HGIQDPQIEIARARDSVERLRDLKVPLIFQEYEMGHEISPRGLDVLSAWLEETVRGRV
                     AAPPAD"
     gene            complement(3718..5409)
                     /locus_tag="VMZ02_03875"
     CDS             complement(3718..5409)
                     /locus_tag="VMZ02_03875"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71101.1"
                     /translation="MSSVALLLSPLWLSWKNSFFRGSHSWTRRMSLTLLAGLFWIGTY
                     LLIRRVLRYFETVYELGPALAYQLLLIILLTFLSMLLFSNLVAALSTFFLARDLDLIH
                     AAPVSPNGFFFARLIATTVNSSWMVLFFSLPIFAAYGSVFGGGITLYLWIAMILPLFL
                     LIPAAIGALVTHLLVYTMPAKRIRDLFFFLGLIAFLIMYFLFRFSQPERLVQPESFGN
                     FMQFLSAMETPSSPYLPSSWLAEILAGVLFKRDTEQPFFFALLTSYAMFLPLAASWCS
                     SLVYLDGWSKAQESRQGRRKLAWLDRLLDVITVPFPAITRALMLKDIKTFLRDSTQWS
                     QLLLLGALIIVYLYNFKVLPLDRSPMPAGTIRTVVSFANLGLAGFVLSAIAVRFAFPA
                     VSLEGKAFWLLQTAPISLRALLWSKFWLNFVPLCVLGELLIFCSNLLLKVPGWMMALS
                     LITIFLMTFGITAIGVGLGATYPKFDYENAAEIPTSFGGAVCMIFSMGFIGLNVMIVA
                     WPIYTLAMRAFNRGRTFVPEYSVIAPSLLIVLAVTVVTVSAALRVGIKHLEQMKE"
     gene            complement(5406..6164)
                     /locus_tag="VMZ02_03880"
     CDS             complement(5406..6164)
                     /locus_tag="VMZ02_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR71102.1"
                     /translation="MIQIKNLSKQYGKLAAVDNLTLEVAAGEIFGFLGPNGAGKTTTI
                     RCIMGILKATSGQVLLNGHNVVSDAQKAKAIAGFIPDRPFIYEKLSGSEFLQFVGKLH
                     RVDPARLSRRSAELLDHLELTPWKDELVESYSHGMKQRLVVCSALIHDPTILVIDEPM
                     VGMDPKGARTLKDLFCALAKNGTAVFLSTHSISVAEEICHRIGIIRKGRLISCGTMSE
                     IHAQTARGNGSLESAFLELTREQELPVPAAQSLI"
     gene            complement(6223..7674)
                     /locus_tag="VMZ02_03885"
     CDS             complement(6223..7674)
                     /locus_tag="VMZ02_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HUR71103.1"
                     /translation="MIVRRSIGVGIVLFWAVMNFLLIRRQSVAPPPLLTLRGSETIIE
                     ASEEWWGIYYRGEKIGYASQSIVPKARGYKLNDYSRINLNLLGTVQPAETRLEMEADE
                     DWILQRFDFALRSKEIGFKARGAVTNNKLSLDIDSAGHRSSREITLTQAPYLLASLKP
                     YVVTQQLEIGKKFYFATFDPSTLSQQVTTIIIEGREQIRIGNRLEPAIKMRQSFAGIS
                     VLSWVDVHGRTLKEESPAGMSMTRQSQAEAKNLPSRSVPSDMTAQAAIPVTTSIPNAK
                     SLSAIQLKLGGVDLANFALDGGRQKLTGDRLMVIREDLTTLTPTAIPVKEPPLQTFLQ
                     PTAFMQSDHPTIKSLAARIVGSESKALLASIKLKDWVYRELAKEPTISIPNALEVLRV
                     KKGDCNEHTVLFNALARAAGIPAKTVVGVVYLRGAFYYHAWSEIWLGRWVALDSVLNQ
                     FPADVTHVKFIEGEIDRQVDILQLIGNLKIEVM"
     gene            7739..8209
                     /locus_tag="VMZ02_03890"
     CDS             7739..8209
                     /locus_tag="VMZ02_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HUR71104.1"
                     /translation="MVAKEISFAADRMLGRLAKWLRILGYDVIYGQHLSGRGLIRAAR
                     QDGRLILTRDRSLRRKQPPPFLFIESNDYVEQLRQVLRVYDLSPCDRLFTRCLVCNSQ
                     LQPRSKQAVEKLVPEYVFASQERFSWCGCCQKIYWPATHHQRMLAALARVAVIS"
     gene            8309..8384
                     /locus_tag="VMZ02_03895"
     tRNA            8309..8384
                     /locus_tag="VMZ02_03895"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8342..8344,aa:Asn,seq:gtt)
BASE COUNT         1939 a         2291 c         2419 g         1861 t
ORIGIN      
        1 aaggaaaaag tctctggcga cgccaaccga tcgcggcgta catccagccg cccgacgtca
       61 actccgacac ccaggagaaa ttgaaaaccg ttttggcggt gcgcgagtat gcgcgcgacg
      121 tcctcaagct caacgtcggc ggcagttacg caagctattc ctacgtcgat cggccggatc
      181 tgacttatgt tgtcatcgct gcaccgcaga ccgagctgcg gccttacacc tggtggtttc
      241 tgatcgtcgg cagagtgccg tacaagggtt acttttccag acaagacgcc gagggggaga
      301 tcgagcgctt gaaggccaaa ggctacgata ccaacctgcg tacttcggct gctttcagca
      361 ccctcggctg gttcgacgat ccgctattat cccaccttct caagtacgac aagctcggcc
      421 tctcagagat agtttttcat gagctgtttc acaatacact ctatgtcagc ggtcagggcg
      481 cgttcaatga aagcgtcgct accttcatcg ggcatcgcgc cgcgatcgat ttttttcgcg
      541 ccaaatttgg cgccgacagc gccgaacaca agcgcgcgat tgaatggtgg agcgaagagc
      601 gcgagttcgg tgcgtatatt ggagagatcg cgcgcgattt aaccgagctc tacaacagtg
      661 ctatcccacg cgaggtcaag ctgcgtctgc gcgaagaggt ttttacccgc agcaaggccg
      721 aatgggcacg gcgcatcgcc gatcggccca tgcaccgttt ccgcggtttt agccaacagg
      781 cgctcaataa cgccgtgctg atgcactatg tggtttactt gaaagacttg gacttgttcg
      841 agtcgttgta tgaatcggcg ggctacaact tgcctaaaac cattgagctg ttgcagcaag
      901 ccgcgggcaa aggcggtgat ccgttcgacg cggtgcgccg actagtaaac cgctttaacc
      961 gggaaaatcg ccaagagcac gatgagaggc ccgatgttaa aaccccagga ctgtcatagc
     1021 aatccggcaa attgccggat tgcttcgata tgcgccgacg gcgaattcaa tcccgctttc
     1081 atggtgttga aactcacatg ggtggcgccg aggttttgcc acgcgagcag ttcttttcgc
     1141 caagcgtccg gagcaccttg gccgtaggaa atacgacctt caatgccgat ggcttgcggc
     1201 cggcggccag cgtctctcgc gtagctgtga atcttgtcga tcatcgcctg gcatttttcg
     1261 tcgggcccga ggagcgggaa ccagccgtcg cccaagcgcg ccaagcggcg caatgcacgc
     1321 tcgtcgctgc cgccgaacca aattggaatc ggcctctgca cgggcagcgg gttgatcccg
     1381 gcatcagtga tcttgtgcca gcgaccctgg aaagtcaccg tggggtttgt ccagagctgg
     1441 cgcaagactt cgatctgctc ttcgcagcgg gcaccgcgat ttttgaagtc ttcacccaaa
     1501 gcttcatact ccacagggtt ccaaccgatt ccgacgccaa agcgcagccg gccgccgctt
     1561 agtacgtcta acccggcggt ttgtttggca accaacacgg tctgccgctg cggcaggatg
     1621 atgatgccgg tgcaaaactc gagtcgctcg gtgacaccgg cgagatagct gaatagaacc
     1681 aacggctcat gaaacatatc caagtggctg tagggtgccc gccaatcggc cctgctcgcc
     1741 gggttggcgc cgaccacgtg gtcataggct agaatatgag agaagcccag ggcttcggcc
     1801 gtttggccgt agtctttgat ataataggga tctgcgccga tttcagtctg cggaaatact
     1861 acgccgattc gcatagagag agcctccgtc gacaagtgga atagtaagac aattctgctt
     1921 ggttttgaac tagagacctt gcctttcggc ctgatcgtgc gcagatttat catctaggaa
     1981 tgttccttga cttaacccag gcgtttcgta ggatgtcgcg cataggagta acggtatgaa
     2041 cggtttacga actactattc ttttggcgct gttgaccgcc ctagttgtgt ggatcggcca
     2101 aatggtcggc ggtccccagg gcgcggtaat ggccttgata ttcgccggtg tcatgaattt
     2161 ttttagctac tggttcagtg acaaaatagt tctcaaaatg tacagggcgc aggaaatttc
     2221 cgccgctgac gaccccgagc tttacgggat agttcaggat ctggccactc gcggcgggtt
     2281 gccgatgccg aaggtctaca tcattccgga agataccccg aatgcctttg ccactggccg
     2341 caatcctgag catgccgcag tggcggtgac ggcaggcatc cgacgtatac tcaataagcg
     2401 cgagctcgcc ggggtgctgg cccatgagct aacccatgtc aaaaaccgcg atatcttggt
     2461 cagcagtatc gctgcgacgc tggcgggggc gatttcctac ttagcgcaca tggcgcagtg
     2521 gggaatgatc ttcggagggg gccgcagccg cgacgatgaa ggcggaggca gcaacatttt
     2581 cggcctgctg ttcatgatga tcgttgcgcc gatcgccgcc atgttaattc agatggcagt
     2641 atctcgctcg cgcgagtata tggccgacac aggcggcgcg aagatttccg gcgatccgtt
     2701 ggccttggcc agcgccttac gaaaactgca catgggcgcg caaaacattc ccttacaggt
     2761 gagcgatgcg accgccactt caacggcgca tatgttcatc gttaacccgc tgagtggcgc
     2821 tgggctggcg aatttattta gcactcatcc ggcgatggaa gagcgcatcg cccgacttga
     2881 agcgatggcg aaagatatga gctacttgca cgcttaacca gtcagtcagc cgggggcgcg
     2941 gccacgcgcc cccgtacagt ttcttccaac caggccgata gaacgtccag cccccgcggg
     3001 ctaatctcgt ggcccatctc atattcttgg aatatcagcg gaaccttgag gtcgcgcaag
     3061 cgctctaccg agtcgcgtgc acgggcgatt tcgatctgcg ggtcttggat gccatgttgt
     3121 atcaacgtag gcagtgactg caccgcggcg ctgatcgcca gccgcggcgt caattctcgc
     3181 ggtaaccacg tgctgagcgc cgctaaaccg gcgaatcgct gtggatgggt caatgccaga
     3241 ctatatgcca tcaccccccc ttggctgaaa ccaagaacgg cgagccgcgt cggatcaagg
     3301 tcataccgct taaaacaatc gtcgacaaac aattgcaaca cactaaggga ggtcaacatg
     3361 ccttcgatgt ccggcacgcc gccggcactc gcaccgtacc acgcgtaacc caccgcttga
     3421 gggccgatgg gagtttcaat cggcgcctgc ggagaaataa tcaggaactt cccgccgccg
     3481 atatacggtg caaggccgag taaatcgaag gcattggcgc cgcggccgtg caaagttaga
     3541 atcgtcggat gcggtcctgg gccggctggt tgatagacgg tatggatgag gttcatgcat
     3601 cgctccaacg atttttttca ttgcactcgc gtaatgtgca atgttttacc tgccgacgtt
     3661 tgaatccgct gctgatatgc gcgggcgaaa tttgcacgtt cttgagactg ccagacgcta
     3721 ttctttcatc tgttctaagt gcttgatgcc gacgcgcaac gcggccgaga ccgttacgac
     3781 ggtgacagct agcacgatca gcagcgaagg tgcaatcacg gaatactcgg gcacgaatgt
     3841 gcgaccacgg ttaaaggcgc gcatggctag cgtgtagata ggccaggcaa cgatcatcac
     3901 gttaaggccg atgaacccca tgctgaagat catgcacacg gcaccaccga agctagttgg
     3961 gatctcggcg gcattttcat aatcgaactt gggataggtg gcgcccaagc cgaccccaat
     4021 ggcggtgatg ccgaaagtca tcaaaaagat cgtgatcagt gacagcgcca tcatccagcc
     4081 gggcactttg agcaataggt tgctacaaaa aatcagcagc tcgccgagca cacacagtgg
     4141 cacgaagttc aaccagaatt tactccatag cagtgctcgc agtgagatcg gcgccgtctg
     4201 cagtagccaa aatgctttgc cttccagact cactgcggga aaagcgaaac gaactgctat
     4261 ggcactgagg acgaatccgg ccaagccaag attggcgaat gatacgaccg tgcggatggt
     4321 tcccgccggc atcggcgagc ggtcgagtgg caggacttta aaattataga gatagacgat
     4381 tatcagagcg ccgagtagta acagctgcga ccactgtgtc gaatcgcgca ggaaggtttt
     4441 gatgtccttg agcatgagcg cgcgcgtgat tgctggaaaa ggcacggtta tgacgtcgag
     4501 caggcggtcc agccaggcga gcttgcgccg gccttgtcgc gattcttgag ctttcgacca
     4561 gccatcgaga tagaccaggc tcgaacacca actggctgcc aacggcagaa acatcgcgta
     4621 gctcgtgagc agcgcaaaga agaagggctg ttcggtgtcg cgcttgaaca acacgccggc
     4681 gaggatctcc gccaaccaac tgctcggcag atagggcgat gacggggttt ccatagcgct
     4741 taggaactgc atgaaattgc cgaacgactc gggctgcacc aggcgttcgg gttgggaaaa
     4801 gcggaacaga aagtacatga tcaaaaaggc gatcagaccg aggaaaaaga acaggtcccg
     4861 gatacgcttg gcgggcatgg tgtacaccaa cagatgagtg accagtgcac cgatggcggc
     4921 cgggatgagc aagaatagcg gcagtatcat cgcgatccac agatagagag tgatgccgcc
     4981 gccgaacacc gaaccgtaag cggcgaagat cggcagtgaa aagaacagca ccatccaaga
     5041 cgaattgacc gtggtggcga tcaagcgagc gaagaaaaag ccgttgggcg ataccggcgc
     5101 ggcgtggatc agatcgagat cacgcgcaag aaaaaacgtc gacaaagccg ccaccaggtt
     5161 gctgaacagc agcatcgaca ggaaggtcag caaaattatc agcagcaatt gataggcgag
     5221 ggcgggcccg agctcgtaga ccgtttcgaa gtagcgcaag acgcggcgga tgagcagata
     5281 cgtgccgatc cagaacaagc cggcgagcag cgttagcgac atccgtcttg tccaagagtg
     5341 gctacctcgg aaaaaactgt ttttccatga cagccacaat ggcgagagca gtaacgcgac
     5401 cgaactcata tgaggctttg cgccgcaggc actggcaact cctgctcgcg ggtgagttcg
     5461 agaaaggcgc tttcgagact accgtttccg cgggctgtct gcgcgtggat ctcgctcatg
     5521 gtcccgcagg atattaagcg acctttgcga atgatgccga tgcgatgaca aatttcctcg
     5581 gcaacgctaa tgctgtgggt cgagagaaaa acggccgtgc cgtttttggc caaagcgcag
     5641 aacagatctt tgagagtgcg cgcccctttg gggtccatcc caaccatcgg ttcgtcgatg
     5701 accagaatcg ttggatcatg aatcaacgcg ctgcaaacta ccagacgctg tttcatgccg
     5761 tgcgagtagc tttcgaccag ctcatccttc cacggggtta attccaggtg atcgaggagt
     5821 tcagcgctgc gcctgcttag tcgcgccggg tcgacccgat gaagtttgcc gacgaactgc
     5881 aaaaactcgc taccgctgag tttttcgtaa ataaatggtc gatcggggat gaagccggca
     5941 atcgccttgg ctttctgagc atcgctgacg acattgtggc cgttcaacag aacctgtccc
     6001 gaagtcgctt tcaggatgcc cattatgcaa cggatcgtcg tagtcttgcc ggcgccgttg
     6061 ggaccgagga agccgaatat ctcacccgct gcgacctcta gtgtcaaatt atctaccgcg
     6121 gcgagcttgc cgtactgttt actcaggttt ttgatttgaa tcattcaatt ttgatttgcg
     6181 cttaaagtga cgagtcgaag tatcgatccg atatcctgtt ggtcacataa cctcaatttt
     6241 caaattgccg ataagttgaa gaatgtcgac ttggcgatcg atttcgccct cgataaattt
     6301 cacatgcgta acatcagcgg gaaattggtt gagcaccgaa tcaagggcga cccaacggcc
     6361 gagccaaatc tccgaccaag catggtagta aaacgcgccg cgcagataaa cgacgccgac
     6421 gactgtcttc gccgggatcc ctgcagcccg cgctaacgcg ttgaatagga cggtgtgttc
     6481 gttacagtcg cctttcttca cgcgcagaac ttccaacgcg ttgggaatac ttatggtagg
     6541 ttccttggca agctcgcggt aaacccagtc ctttaacttt atcgaagcca gaagagcttt
     6601 actttccgaa ccgacgatgc gcgccgcaag cgacttgatc gtcggatgat ccgactgcat
     6661 gaaagcggtg ggctgcaaaa aggtttgcag cggaggttct ttgaccggaa tcgccgtagg
     6721 cgtgagtgtg gtgagatctt ctcgaatgac catcaagcgg tcgccggtga gtttttgccg
     6781 tcctccatcg agcgcgaagt tcgctagatc cacacctccg agttttaatt gtatcgctga
     6841 cagcgattta gcattgggta tggacgtagt gaccgggatc gccgcctggg cggtcatgtc
     6901 cgacggcacg gagcgagacg gcagattttt cgcttcggct tggctttgcc gggtcatcga
     6961 catgcccgca ggcgattctt ctttcaacgt ccggccatgg acatcgaccc acgagagaac
     7021 ggaaattcca gcgaagcttt gacgcatttt gatcgccggt tcgaggcggt tgccgatgcg
     7081 gatctgttcg cgtccttcga taattatggt ggtaacctgc tgcgacagcg tcgatggatc
     7141 gaaagtcgcg aagtagaatt ttttgccgat ttctaactgc tgggtgacaa catatggttt
     7201 gagcgacgcg agcaaatagg gtgcctgggt cagagtgatc tcgcgtgacg agcggtggcc
     7261 ggcggagtcg atatccagag acagcttgtt attcgtaacc gcgccgcgcg ctttgaagcc
     7321 gatctccttg gaacggaggg cgaaatcgaa gcgctgcaaa atccaatctt cgtcggcttc
     7381 catctctagc cgggtctccg cgggctggac cgtccccagt aaatttaaat ttattcggga
     7441 atagtcattc agtttatatc ccctagcttt cgggacgatc gactgggagg catagccgat
     7501 cttttcaccg cgatagtaga taccccacca ttcttcgctg gcctcgatga ttgtttcgct
     7561 gccgcgtagc gtcaggagcg gcggcggggc aacgctctgg cgtctgatga gaaggaaatt
     7621 catcacggcc cagaacagaa caatgccgac gccaatggat cgcctaacaa tcataatgta
     7681 gaacctatat ggtaaggctc cggctctcgt gaatcaatgt gaatcgtaat gtcctcttat
     7741 ggttgctaaa gaaatatcgt ttgccgcaga tcgcatgtta ggccggctcg ccaaatggct
     7801 gcgcattctt ggctacgatg ttatatacgg ccagcatctc tctgggcgtg gtttgattcg
     7861 cgccgcacgc caggacggcc ggttaattct gacacgtgac cgcagtctgc ggcgtaagca
     7921 gccgccgccg tttctgttca tcgaaagcaa cgactacgtc gaacaactgc ggcaggtgct
     7981 gcgtgtatac gatttgtctc cctgtgaccg cctatttacc cgctgtttgg tatgcaactc
     8041 gcaattgcag ccgcgttcca agcaagcggt ggaaaaactg gtaccggaat atgtgtttgc
     8101 ctcgcaggaa cgcttttcgt ggtgcggttg ctgtcagaag atttattggc cggccacgca
     8161 tcatcagaga atgcttgccg cgcttgcaag agttgccgtc atttcgtgaa acgaaacaca
     8221 cgttacgctt gttggcgtca ccagtaaatt gagctggaac tggaaatcca tccgagcgta
     8281 tgctaaaaac tgtccctgtt cttttgcttc cccggtagct cagttggtag agcaggtgac
     8341 tgttaatcac cgggtcgggg gttcgagtcc ctcccgggga gccagctatt catactcgaa
     8401 accttgtgtt gagctgtccg ctaccattcg tttcctcgct tttgtcgatg tagttttggc
     8461 cattaaaatt ttgatgccgt gaatgaattt ttcagaccac cgtgactcgg
//
LOCUS       DATLCH010000133         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_398994, whole genome shotgun sequence.
ACCESSION   DATLCH010000133 DATLCH010000000
VERSION     DATLCH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398994"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..1986)
                     /locus_tag="VMZ02_03900"
     CDS             complement(415..1986)
                     /locus_tag="VMZ02_03900"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR71105.1"
                     /translation="MAQAKRDGASPEEARDLRSAIEWMKKEGDIIVTDKEVDPNLELT
                     GIQKKLDGGCPILFNNVKGKPQHRCITNLFGDMNVINKMFGWKDHADRTRKLAYAITH
                     PIAPIVVDQKDAPVQEEVYEKPAEANDFVVPIRHTDIEPELTVGSGNRLISGEYFNGG
                     TDLGYNRMNFRWGNVGTFQISPGSHMWQVVSKYYKDNKMVPITMNFGLPVAGSLLAGA
                     GFDYVILPQGCDEVGIAGAVQGAPMRLVKARTVDAYALADAEVILEGYVNPRDRRFET
                     AESEKAGIQGRFHFHPEWAGYMGKAYKAPTFHVTAVTTRKREGKPIIFPLGVHTLDDH
                     NIDTTVREASMFELCERMQPGIIMDVNIPYCMTDWGGAIIQVKKRNRIEEGWQRNFMA
                     AILATSQGSRLVVAVSEDSDPYDMDDIMWNLTTRVNPKTDIINPLPGGRGQTFMPAER
                     MTAGEREWTASNTNFEGGMGIDATVPFGYESDFMRPVYPVDRVDLKKWFSDKDIQNAK
                     SRMQGWVHSLARTGR"
     gene            2296..>2650
                     /locus_tag="VMZ02_03905"
     CDS             2296..>2650
                     /locus_tag="VMZ02_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71106.1"
                     /translation="MTVGVPRSKQDWLLLVGLFCFFFSGAAGLIYQVVWTRMLSQIFG
                     NTTYAIATVLSAFMAGLAIGSYWFGKIADRGKNDFLLYGILEAGVGVYGFLVPWFFAG
                     AQKVYSPIFSLNESHP"
BASE COUNT          552 a          704 c          707 g          687 t
ORIGIN      
        1 ttatttttgg cccggtgtga ctctttgctc gaagccggac aaaccgaaac caaccaggca
       61 gtggccacgc taatacagca gggtggcaat tgctggtgaa actctttcgc agtccttgct
      121 gttggaatta tttcaccaat cggaaggttg cgccgttggt tcgaaatggc ggctatttat
      181 agtgtcattt cgagcgcagc gagaaatcta ttcgcttcac tttgtcgaag aaatgtgaca
      241 acacaagttg ccattcgtca ctcccgacgc gatttggatt atatcctcgc ggacagtttt
      301 cccccatcgc aatatcggtt cgtcaaacac ctccacacgg ctgcgacgaa tgaaaaaaag
      361 gggcaaggaa aacttccttg cccctttttt aaagctgact tgtataacta aaacttatcg
      421 gccggtacgc gccagcgaat ggacccatcc ctgcatgcgg ctcttggcat tttggatatc
      481 tttgtcgctg aaccattttt tcagatcgac cctgtcgacg gggtaaaccg ggcgcatgaa
      541 atcgctttca tacccgaagg gaacggtcgc gtcgatgccc atgccgcctt caaaattagt
      601 gtttgaggcg gtccattctc gttcaccggc tgtcatacgc tcggcaggca tgaaggtctg
      661 accgcggccg ccgggaagcg gattgatgat gtcagtctta ggattgacgc gcgtcgtcag
      721 gttccacatg atgtcgtcca tgtcataggg atcggaatct tcgctcactg cgaccaccaa
      781 gcgcgagcct tgcgacgtcg cgaggatcgc cgccatgaaa ttgcgctgcc aaccctcctc
      841 gatgcgattg cgttttttca cctgaatgat cgcgccgccc cagtcggtca tgcagtaagg
      901 aatgttgacg tccatgatga tgccgggctg cattctctcg cagagttcga acatcgacgc
      961 ttcgcggacc gtggtatcga tgttgtgatc gtcgagagta tgcacgccga gggggaaaat
     1021 aatcggtttg ccttcgcgtt tacgcgtggt aacggccgtc acgtggaagg tcggcgcttt
     1081 gtaagctttg cccatgtagc ccgcccattc gggatggaaa tggaaacggc cctggatgcc
     1141 ggctttttcc gattcagcgg tttcgaaccg gcggtcacgc gggttgacat agccttcgag
     1201 gatcacttcc gcatcggcca gcgcgtaggc gtcaaccgta cgcgctttca ccaatcgcat
     1261 gggcgcgcct tgcacggcgc cggcgatgcc gacttcatcg cagccctgcg gcaggattac
     1321 gtaatcgaat cccgcgccgg cgagcagcga gccggcgact ggcaacccga agttcatggt
     1381 aatcggcacc atcttgttat ctttgtaata cttgctgacg acctgccaca tgtgcgagcc
     1441 gggggagatt tgaaaagtgc cgacattacc ccaacgaaaa ttcatgcggt tgtaaccgag
     1501 atccgtgcca ccattgaaat attcgcccga gatcaaacgg ttaccggagc cgacggttaa
     1561 ttcaggctcg atatcggtgt ggcggatcgg caccacgaaa tcgtttgctt ccgccggttt
     1621 ctcgtaaact tcttcctgca ccggcgcatc tttctgatcg acaacgatcg gcgcgatcgg
     1681 gtgggtgatg gcgtatgcga gtttgcgcgt gcgatcggca tgatctttcc agccgaacat
     1741 cttgttgatg acgttcatgt cgccaaacaa gttggtgata caacggtgct gcggtttacc
     1801 cttaacgttg ttgaaaagaa tagggcagcc gccgtcgagc tttttctgaa tgccggtgag
     1861 ttccagattg gggtcgactt ctttgtcggt gacgatgatg tcgccttctt ttttcatcca
     1921 ctcaatcgcg ctgcgtagat ctctagcttc ttccggcgat gcgccgtctc gcttagcttg
     1981 tgccattctt gcctccgagt gattggttac tgctctcaat atttaacgcg acaaagttct
     2041 aaaaagatgc tgtatcgatc gttacaaact tcttccggct gacgaaatcg acggggattt
     2101 aatccgactc agcgtgctga atggccctta cggatataca gaatcttagc caaagtcaac
     2161 gaacgcttca gccggcgcga acgtctacat ttagcatccc ttcaaggcgc tcgcagcgca
     2221 tctgcgactt tacagttaat taccggcctg ctatctgtgc tgggattatc tcgcgccgtc
     2281 aatgagggag agtttatgac cgtaggcgtt ccgcgttcca aacaagattg gctcttgctg
     2341 gtcggacttt tttgtttctt tttttccggc gcagccggac tgatctacca agtggtctgg
     2401 acccggatgc tgtcgcagat ctttggcaac accacctacg cgattgccac ggtactctcg
     2461 gctttcatgg caggattggc aatcggtagt tactggttcg gcaaaatcgc cgatcgcggg
     2521 aagaacgatt ttctgttgta cggcatactc gaagccggcg tcggtgttta tgggtttctc
     2581 gtgccgtggt tttttgccgg cgcgcaaaaa gtttatagtc cgatattttc tctcaatgag
     2641 agtcatccgt
//
LOCUS       DATLCH010000134         5901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3191830, whole genome shotgun sequence.
ACCESSION   DATLCH010000134 DATLCH010000000
VERSION     DATLCH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3191830"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VMZ02_03910"
     CDS             complement(<1..222)
                     /locus_tag="VMZ02_03910"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HUR71107.1"
                     /translation="MKRVELVGLDGIAEIHTGESLGQLICDACERQQFPLADGDILVV
                     AQKIVSKAEGQMIALATVKPSARAQELASE"
     gene            complement(219..908)
                     /gene="cofC"
                     /locus_tag="VMZ02_03915"
     CDS             complement(219..908)
                     /gene="cofC"
                     /locus_tag="VMZ02_03915"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HUR71108.1"
                     /translation="MNVAALLPVKGFHNAKQRLSPLLGIGARELLAETMFRDVLRQVR
                     LALGLAGTFVVTGDDKVAAIAAAAGAAVIRENAENGETSAVDFARLELKQSGCQAVLI
                     IPADMPLMRAEDIDAVLAQVPADAAAPYALLVPSHDKLGTNALLLAPPDVIQLRFGHD
                     SFTFHMGQVAERGMPAHFFENENIALDIDEPKDLERFLAYGYQGGESTRVARELLAAD
                     VAPTRQGSGGT"
     gene            complement(927..1853)
                     /gene="cofD"
                     /locus_tag="VMZ02_03920"
     CDS             complement(927..1853)
                     /gene="cofD"
                     /locus_tag="VMZ02_03920"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HUR71109.1"
                     /translation="MLVVLTGGTGGAKLIDGLAAEIDPSELTIICNTGDDAIFHGLYV
                     APDIDTIVYTLAGLNDAAKGWGIESDTFTVLEQLRRLGNDAWFNIGDKDLATHISRTK
                     WLNEGLKLSQVTERIRRQLGVEPRILPMSDQRVETRVQTPQGEISFQEFFVKERWKCE
                     VNSVRFAGAESADPAPGVLEALHSAAAILICPSNPITSIGPILSVPGVRRALEQSHAP
                     VVGVSPIIGAAAVSGPAHKLMTASGFEASVVGVAKFYQAFLDTLLIADEDAACAVVME
                     ALKIAPVCTDIRMDSIADKRRLARQVLASVQK"
     gene            complement(1853..3037)
                     /gene="cofG"
                     /locus_tag="VMZ02_03925"
     CDS             complement(1853..3037)
                     /gene="cofG"
                     /locus_tag="VMZ02_03925"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HUR71110.1"
                     /translation="MSRSEALSFVEAEGDALRQLLSTAAELRDRHKGRVVTFSPKVFV
                     PLTNLCRDFCGYCTFRKAPDEPGAKTMTADEVLRVVRQGKTLGCTEVLLSLGDKPEAI
                     YPEMKRFLTDRGHQRTLEYLHEICRVVLEETGLLPHANAGIMGRGDLQRLKQVNPSMG
                     LMLESVSERLMLVGAAHDNAPDKKPAARLRTLKLAGELRIPFTTGILIGIGENWPERI
                     DSLLAIRALHERYGHIQEVIVQNFRAKPTIPMRAHPDATQEEMVKTIALARLILGGAM
                     NIQAPPNLTPDGYELYLDAGINDWGGVSPLTPDFINPEAPWPALRLLQAKSAQAGFEL
                     KARLPVYPEFIRQGDKFLPPHMRSYIEPLCDADGLVKAGGPLGHTPVPQSCKIPNAAG
                     LC"
     gene            3210..4409
                     /locus_tag="VMZ02_03930"
     CDS             3210..4409
                     /locus_tag="VMZ02_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71111.1"
                     /translation="MDNFDNVLVVDADGHVYEGNIDLRPRMPEKWRSQAPVNMKDNEG
                     NSRILLEGRMWSASQGLGPGVSAPMTDKARGFRPGMVDPVARLEDMDAEGIDVAILFG
                     TQIALTVNGLMSKELSAVLCHAVNDWLIEYCSADKKRLVGVGLIPCQDPLAAVKELEF
                     LAKSGIVTAMLPTNVYGLNNMGDRMFDPIYGCAQSLGMALSVHPQTGHDGVPGVSGVM
                     GAGSERLRKYVYVHMTAFPFELMLSMMHMIGEGVFDRYPKLRVGFMEGGAGWLPFWAE
                     RFDEHIEKLAPQMPDLKRRPSEIIRGEQITLTCESEETGLDRVFAANGENTVMYASDY
                     CHWDCHFPYSVKDVIDCKDLSFTQKEKLLNKTAVDFFKLKNLPQANALKLARQSWSNG
                     KAQAANA"
     gene            4421..5368
                     /locus_tag="VMZ02_03935"
     CDS             4421..5368
                     /locus_tag="VMZ02_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71112.1"
                     /translation="MKLKVFIGAFLWFAQSLVASQSNGAETPYKIRIGFPSLAFSYMP
                     FYVAQEKGIYKKYNIESEYIQMTTSIQPQAVVNGNINYLTSVSTGISAAVANLPLLIL
                     MNFSDTSPWVLITHKDINKPQDLIGKTVALSGIRTSPYYFFQAFLKKHEIKDKEINII
                     NTGGTADSFRALTSNRVVATIVTPPFDDKAVSLGYKKFMPLGELADIPYVGLVTSQNE
                     IKTNRESVRRTVAAVFESLAWLRANRADSVKMIIDKFKVTQQEAEGTYGTLMGIMNKD
                     SRLNPKVARGYIDILRQERTIPPDYDPMKHTDFSLLPRS"
     gene            5438..>5901
                     /locus_tag="VMZ02_03940"
     CDS             5438..>5901
                     /locus_tag="VMZ02_03940"
                     /inference="COORDINATES: protein motif:HMM:NF022081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUR71113.1"
                     /translation="MNWLVAIVLACCSVSSVFAQPAQEQLKVATRIVKPFVFEENSRL
                     TGFSVELWNEIAKQLNVATEYVVKPTVKELLSSVNNHEAALGIAAISITAERELHWDF
                     SQPMFEAGLQILAPVHGGRATLIRSIFANLLSTAVLPYVLAVASILLIIAHLV"
BASE COUNT         1327 a         1722 c         1595 g         1257 t
ORIGIN      
        1 ttcactggcc agctcctgcg cccgcgccga aggcttaacc gttgccagag caatcatttg
       61 gccttcggcc ttggaaacaa tcttttgcgc caccacgaga atatcgccgt cggcgagtgg
      121 aaactgctgc cgctcgcagg catcgcatat aagctgtccg agggattcgc cggtgtgaat
      181 ctcggcgatc ccgtccaagc cgacaagctc cacccttttc atgtgccccc tgagccctgg
      241 cgcgtcggcg caacgtcggc agcgagcaac tcccgcgcaa cacgggtcga ctcgccgccc
      301 tgataaccgt aggcaagaaa ccgttcgaga tccttcggtt catcgatatc gagagcgata
      361 ttctcgttct cgaaaaaatg cgccggcatt cctcgctcgg ctacctggcc catatgaaaa
      421 gtgaaactat catggccaaa gcgcagttga atcacatccg gcggcgccaa caacagcgcg
      481 ttggtgccga gcttgtcatg ggacggcacc aataaggcgt agggcgcagc cgcatcggct
      541 ggaacctgcg ccagcaccgc gtcgatatct tcggcgcgca tcaacggcat atcggcggga
      601 atgatcagta ccgcttggca accgctctgc ttcaactcga gacgggcaaa atcgacggcg
      661 ctggtctcgc cattctcggc gttctcgcgg atcacagccg cacccgccgc cgccgcaata
      721 gcggcaacct tatcgtcgcc ggtaacgaca aatgttccgg ccagcccaag ggcgagccgc
      781 acctggcgca ggacatcgcg gaacatggtt tccgccaaaa gttcacgcgc accaataccc
      841 agcagcgggc tcaaacgttg cttggcgttg tgaaaacctt taaccggcag cagtgcggcg
      901 acattcatgg aaagaaaatt cttaatctat ttttgaacgg acgccaagac ttggcgcgcc
      961 aagcgtctct tgtcggctat gctatccata cgaatatcgg tacacaccgg cgcaattttg
     1021 agcgcttcca tgaccactgc gcaagccgcg tcttcatcgg caatcaagag agtgtcgaga
     1081 aatgcctggt aaaactttgc cacgccaacg accgaagctt cgaagccgct ggcggtcatc
     1141 agcttatgcg ccgggccgga aaccgcggcg gcgccaatga tggggctaac gccaacgact
     1201 ggggcatggc tttgttcgag agcgcggcgg acaccgggaa cggataagat cgggccgata
     1261 ctggtaattg ggttactggg gcaaatcaag atggctgcgg cactgtgcag cgcctccagc
     1321 acaccaggcg cggggtcggc ggattctgcg ccggcaaaac gaacggaatt tacttcgcac
     1381 ttccaacgct ctttgacgaa aaactcctga aacgatatct caccttgcgg cgtctgcacg
     1441 cgggtctcga cacgctgatc cgacattggc aagattcttg gctcgactcc caactgccgg
     1501 cgtatacgct cggtcacctg agacagtttc aaaccttcat tgagccactt ggtccggcta
     1561 atgtgcgtcg ccaaatcttt gtcgccgatg ttgaaccatg cgtcgttgcc gagacgccgc
     1621 agctgttcga gcacagtaaa tgtgtcgctc tcgatgcccc atcctttggc ggcatcgttt
     1681 agacccgcca gcgtgtagac gatcgtgtcg atatccggcg cgacgtacaa gccatgaaaa
     1741 atcgcgtcgt caccggtgtt acagatgatc gtcaattcgg acggatcgat ttccgctgcc
     1801 aagccgtcga tcaatttcgc gccgccggta cccccggtga gaacaaccag catcagcaaa
     1861 gcccggcggc atttgggatt ttgcaggact gcggcacagg agtatggccc agcggaccgc
     1921 ccgcttttac taatccatcg gcatcgcata gcggctcgat gtaagagcgc atatgcggcg
     1981 gcaaaaactt gtcgccttgg cggataaact cgggatagac gggcagccgc gctttcagtt
     2041 cgaatcccgc ctgcgcgctt ttcgcttgca gcaatcgcaa cgccggccac ggcgcttctg
     2101 ggttaatgaa atcgggcgtc agcggcgaca cgccgcccca atcgttgatg ccggcatcca
     2161 gataaagctc gtaaccgtcc ggcgtcaaat tagggggcgc ttggatgttc atcgcgccgc
     2221 cgaggatcaa gcgcgccagc gcgatggttt tgaccatctc ctcttgagtc gcatcgggat
     2281 gagcgcgcat gggaattgtc ggtttggcgc ggaagttctg cacgatgact tcctgaatgt
     2341 gaccgtagcg ttcatgcagc gcgcgaattg ccaataagga atcgatccgt tccggccagt
     2401 tttcgccgat gccgatgaga atgccggtgg tgaaaggaat gcgcagctcg ccggccagct
     2461 ttagtgtgcg cagccgcgcc gctggtttct tgtcgggagc attatcatgc gcggcgccga
     2521 caagcatcag gcgttcgctg acgctttcaa gcatcagccc catgctcgga ttcacttgct
     2581 tcaaccgctg caaatcaccg cgtcccatga tgccggcgtt ggcgtggggc aacagacccg
     2641 tctcttcgag caccacccgg cagatttcat ggaggtattc cagcgtgcgc tgatggcccc
     2701 ggtcggtcaa aaatcgcttc atctccgggt aaattgcctc aggtttgtca ccgaggctga
     2761 gcagcacttc ggtgcaaccg agggttttgc cttggcgcac cacccgcaag acctcgtccg
     2821 ccgtcatggt tttcgcaccg ggctcgtcag gcgccttgcg aaaagtgcaa tagccgcaaa
     2881 aatcccggca taagttggtc agcggcacaa aaaccttcgg agaaaaagta accacccggc
     2941 ccttgtgccg atcacgcagc tcagccgccg tcgacagcag ttgccgtagc gcatctcctt
     3001 cagcttcgac aaagctcaga gcctcggaac gggacatggg tttgccctgt cggcagtctc
     3061 gaagaatggc ttgcatctgt catggttgaa tatccaattg gccgccggta ttcaaaccgt
     3121 cacgaggcgt cgtgcgcgac tattctagtt gccctcgaga gttttgctgt gctagttctc
     3181 gtacgatcga caagcgggag gaattcgccg tggataattt tgataatgtt ttagtcgtcg
     3241 acgccgatgg ccatgtttat gaaggaaata tcgacctgcg gccgcgcatg ccggaaaaat
     3301 ggcgctcaca agcgccggtt aacatgaaag acaacgaagg caactcgcgc atactgctcg
     3361 aaggccgcat gtggtccgcc tcccagggac ttggcccagg ggtgtcggca ccgatgaccg
     3421 acaaagcgcg tggttttcgc ccaggcatgg tcgatcccgt agcacggctc gaggatatgg
     3481 acgccgaagg catcgatgtt gcgattctct ttggtaccca gatcgccctc actgtaaacg
     3541 gtttaatgag caaggaactg tcggcagtgc tgtgccacgc ggtcaacgat tggctgatcg
     3601 agtattgttc cgccgataaa aagcgcttgg tcggcgtcgg cttgattccc tgccaggacc
     3661 cgctcgccgc cgtaaaagaa ctggagtttc tcgctaagtc cggcatcgtc actgcgatgc
     3721 tgccaaccaa cgtctatggg ttaaacaaca tgggcgatcg catgttcgat ccaatttacg
     3781 gttgcgcgca gtcacttggg atggcgctta gcgtccatcc gcaaaccggc catgacggcg
     3841 ttcccggcgt atcgggagtg atgggcgccg gcagcgagcg attgcgcaaa tatgtttacg
     3901 ttcatatgac tgcgtttccg ttcgaattga tgttgtcgat gatgcacatg atcggcgaag
     3961 gcgttttcga tcgttatccg aagctccggg tcggcttcat ggaaggtggc gccggctggc
     4021 tgccattttg ggccgaacgc ttcgacgaac acattgaaaa actggcgccg cagatgcccg
     4081 acttaaaacg ccggccaagc gaaatcattc gcggcgaaca gatcacgctt acctgcgaat
     4141 cggaggaaac tggtctcgat cgcgtgttcg ccgccaacgg tgagaacacg gtcatgtatg
     4201 cgtccgacta ttgccactgg gactgccact ttccgtactc ggtcaaggat gtgattgact
     4261 gcaaagactt gagctttacc cagaaagaaa agctgcttaa caaaactgcc gtcgatttct
     4321 tcaagctcaa gaacctgccg caggccaatg ccctcaagct ggcgcggcaa agttggagca
     4381 acggcaaagc ccaggcagcg aacgcgtgag ggacggtaaa atgaaattga aagtttttat
     4441 cggtgccttc ctttggttcg ctcagagctt agttgcaagt caatctaatg gagcggaaac
     4501 tccatacaaa attcgtatcg gctttccatc cctggcgttt tcctacatgc ccttctacgt
     4561 cgcccaggag aaaggcatct acaagaaata caacatcgag tccgaataca tacagatgac
     4621 cacctcgatc caaccacagg ccgtggtcaa cggtaacatc aactatctca cttcggtttc
     4681 gaccgggata tccgctgcag tagccaactt acctttgctg attctgatga atttctccga
     4741 cacctcccct tgggttttga tcacgcacaa agatatcaac aagccgcagg atctgattgg
     4801 caaaaccgtc gcgctttcgg gtatccgcac atcgccgtat tatttcttcc aggcctttct
     4861 taagaagcac gagatcaagg acaaggaaat taatatcatc aacaccggag gcaccgccga
     4921 tagttttcgc gccttgacca gcaaccgagt ggtggcgacc attgtaacgc cgccgttcga
     4981 tgacaaggca gtatcgcttg gttataaaaa attcatgccg ttaggcgaac tagccgatat
     5041 cccctatgtc ggtctggtga cctcacaaaa cgagatcaaa accaaccgcg aatctgtgcg
     5101 gcgtaccgtc gcggcggtct tcgaatcctt ggcatggttg cgcgccaacc gtgccgatag
     5161 cgttaagatg atcatcgaca agttcaaagt aacgcagcaa gaagccgagg ggacgtacgg
     5221 gaccttgatg ggcatcatga acaaagacag ccgtctgaat ccaaaagtag cccgcggcta
     5281 catcgatatc ctgcgccagg agcgtaccat accgcccgac tatgacccga tgaagcacac
     5341 cgacttttct ttgctgccgc gcagctgatc ttagcgctac tctgtgacgc ctggccactc
     5401 actcacaaac aacggcgtaa ctttaccggt aacggcagtg aattggcttg tcgcaattgt
     5461 ccttgcgtgt tgctccgtct ccagcgtctt cgcccagcca gcgcaagagc aactcaaagt
     5521 agccacgcgg atcgtcaaac catttgtatt cgaagaaaac agcagactca ccggcttcag
     5581 cgtcgaactc tggaatgaga tcgccaagca gctcaacgtc gcaactgagt acgtcgtcaa
     5641 gccgacggtc aaagaattgc tctccagtgt caacaatcat gaagccgctc tcggcattgc
     5701 ggcaatttcg atcactgccg aacgggagtt gcactgggac ttctcccaac ccatgttcga
     5761 agccggcctg caaatcctcg caccggttca cggcggtcgg gccaccctga tcagatcgat
     5821 tttcgccaat ctgttgtcaa ccgcagtgct accctacgtc ttggcggtgg cttctattct
     5881 gctcatcatc gcccatctag t
//
LOCUS       DATLCH010000135         9705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4106730, whole genome shotgun sequence.
ACCESSION   DATLCH010000135 DATLCH010000000
VERSION     DATLCH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4106730"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..654)
                     /locus_tag="VMZ02_03945"
     CDS             complement(73..654)
                     /locus_tag="VMZ02_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71114.1"
                     /translation="MPLRLTAQMKQRITRRTLAMVQRSLGYFPELKDAAITIGYTRKH
                     LGSATVIYRKHELYRLIVRLRVRKVTYHTIGHELTHLVQGLGYGDRFGARRADPERIP
                     TGEIQCDIWTLARDPLFLDDPPTYIKMPRSMREHWPDFAESVRALCIAAIDKRHTQRQ
                     YIQWLEKEIRQLAKAPRCEQSTGSAQLFLPFVI"
     gene            731..1648
                     /gene="era"
                     /locus_tag="VMZ02_03950"
     CDS             731..1648
                     /gene="era"
                     /locus_tag="VMZ02_03950"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HUR71115.1"
                     /translation="MPDDINKNKENQPFHCGYVAIIGRPNVGKSTLLNHILGEKVAIV
                     SPKPQTTRNRITGIRTTESSQIVFLDTPGIHSGHSLMNRRMVDTALQTLDEVDGVVWL
                     LDAHDKIKQEEERIAETLRGVETPVLILINKIDLVSKGKLLPLMERCAQLLPDKEIVP
                     VSALKGDGVQLVLGMIDKWLPEAPPFFTEGEYTDQTERFLASEIVREKVFLLTREEIP
                     YGVAVTIDEFTEKTEKNLIVIAATIHTERDSHKGILIGKRGAMLKEIGQQAREELEKI
                     LGCRIFLELFIRVDPNWTQNPNSLADMGL"
     gene            1717..3081
                     /gene="der"
                     /locus_tag="VMZ02_03955"
                     /pseudo
     CDS             1717..3081
                     /gene="der"
                     /locus_tag="VMZ02_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
     gene            3319..4431
                     /gene="dprA"
                     /locus_tag="VMZ02_03960"
     CDS             3319..4431
                     /gene="dprA"
                     /locus_tag="VMZ02_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HUR71116.1"
                     /translation="MRMHDELVPWLALTRVKGLGCVSFKKLADHFGDPTQAFAAAPSE
                     LDAIDGLQRDAIEGLVSFSQWSEVDNELKRIRSAGVTLIRYTDGAYPARLRAIADPPP
                     LLYVKGAFAPCDERAMAIVGSRSASDYGRRVARDLARGLASHGFTVVSGLARGIDGMA
                     HETALDFGARTIAVLGSGVERAYPPEHDRLYRRIAEQGAIISELPIGTKPMAFNFPAR
                     NRLISGLSLGVVVVEATEKSGSLITASLALDQGREVFAVPGEVGSSRSRGAHRLIRQG
                     AKLVEGVDDIIEEIAPQLARHEGTNIKQEARQLPQSSSAAVQKIFAMLQERSLQIDEI
                     IEHSGLVPAQVLGILLDLELHGYLRQLPGKVYIVER"
     gene            4477..6786
                     /gene="topA"
                     /locus_tag="VMZ02_03965"
     CDS             4477..6786
                     /gene="topA"
                     /locus_tag="VMZ02_03965"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HUR71117.1"
                     /translation="MAAKNLVIVESPAKAKTLEKYLGRDYQVKASVGHVIDLPKSKLG
                     VDIKKDFAPDFTVIQGKKKVIDDLKKAAKGKANIYLASDPDREGEAIAWHIASQVVKN
                     HKHVHRVLINEITKKAVQAAIANPLALDRNKFDAQVARRVLDRLVGYKISPLLWTKVR
                     RGLSAGRVQSVAVRLVCEREKDIRAFDPVEYWSLTATLEGRLPPPFKAKLVQWQDQKV
                     DNKKFRLENDATVQSIVKSLEGASWIIGEVEKKERRRYPAPPFVTSKLQQEASRKLGF
                     QPKRTMQLAQQLYEGVELGTEGSVGLITYMRTDSTRISDDAIAALRGFIQGSYGKNYV
                     PGKPNTFRSKKGAQDAHEAIRPTALEYTPDKVRRYLRRDMFQLYSLIWDRFVASQMVP
                     AVYDQTAFEIPVNEAVFRATGQQIKFDGFMKVYIEGRDERATQNNDDEEEETQELDGV
                     LPDLHQGDALTLQAMEPRQHFTQPPPRFTQASLIKELDEKGIGRPSTYAAIISNILDR
                     EYVAQNEGRQLVPTDLGFLVNDLLVESFPDILNVEFTASMEDELDKIEDGKEKWTKAM
                     KRFYTPFARDLKKAEKEMRDVKRQEVPTDIACEKCGKLMVVKWGRNGEFIACPSYPEC
                     KNTKNFKRDDKGEIEIAAEEEVDEACDKCGKPMLLRFGKFGKFLGCSGYPECKNIQPL
                     QKPVDLGIKCPECREGNIKERKSRWGKMFYGCDRYPECKFATWDKPMPTPCPDCDSPI
                     LVEKVLKRTGRTHRCYKKECGYSIQVAEQ"
     gene            7021..8388
                     /gene="trmFO"
                     /locus_tag="VMZ02_03970"
     CDS             7021..8388
                     /gene="trmFO"
                     /locus_tag="VMZ02_03970"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HUR71118.1"
                     /translation="MKNSVCIIGGGLAGSEAAWQLARRGVAVEIYEMRPQRMTEAHST
                     AHFGELVCSNSLRSDSLSAPAGLLKAEMRQFDSLVIRVAEANRVPAGSALAVDRERFA
                     VGLTAAVETLPRVKVIRQEVKTIPDGGIVILATGPLTSPELAGDLTAKLGEEHLYFYD
                     AISPIVSAESIDMNIAYRASRYDKGGDDYLNLPMTREEYYHFIEGLLTAKRVPTHSFE
                     RFVPFEGCMPIEEMADRGKDTLAFGPMRAVGLIDPRTGRRPHAVVQLRQENRERTLYN
                     IVGFQTKMTYPEQRRVFGLIPGLSDADFVRLGSLHRNTFINAPQHLAPTLQWRRRESL
                     FFAGQMTGVEGYIESAAVGMLAGINAARLLSALPLLVPPPTTALGALLRYITDPERKR
                     FQPMNVNFGLLPPLSVRLRGKAKKEMHSQRALADLAQWLDQTGVDGRAAEFQVPSNSA
                     IVAVP"
     gene            8385..9320
                     /locus_tag="VMZ02_03975"
     CDS             8385..9320
                     /locus_tag="VMZ02_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HUR71119.1"
                     /translation="MSPRRNAFIDVGTNTILCLIADIRDTGRFRVLDDLAEIARLGQG
                     VDQTGVISAGGEERALAVLARYRDRCKDLGVEEITAVGTSALRDARNSDLVRARIRDA
                     LGINIRVISGAEEAGYSFRAVQRGLSLSARELLAIDIGGGSTEFIFGNENGVSRAVSI
                     DIGSVRLTERYLHSDPVASGEVTAMGAAVEAHLSASTNEIISQASSATLVGIAGTFTT
                     LAAIEKKLARYSHSQVHGSVLDLTEVKRQVVMLQKCSIAVRKQIAGLEPKRADVIYAG
                     AYLVEKIMELFGAKQVVVSDQGVRYGLLYEAADSL"
     gene            9478..>9705
                     /locus_tag="VMZ02_03980"
     CDS             9478..>9705
                     /locus_tag="VMZ02_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71120.1"
                     /translation="MSIKTEKIWLDGELVRWEDAKIHVLSHALHYGTGYFEGIRCYEL
                     ADGSSAIFRLTEHMQRLVDSGKILGFPLPYSV"
BASE COUNT         2187 a         2547 c         2844 g         2127 t
ORIGIN      
        1 tcctaatatg tgacggtttc cacacacgcc ggctaccctc tttccaccca acgagactgc
       61 tgccaagatt actcagatca cgaagggcaa aaacagctgc gctgagccgg ttgattgctc
      121 gcaccgcggt gctttcgcca attgcctaat ttctttttcc agccattgga tgtactggcg
      181 ctgagtatgg cgtttgtcga tggctgcgat gcagagggcc cgaacggact cggcgaagtc
      241 cggccagtgc tcgcgcatac tgcgcggcat tttgatgtag gtcggtgggt cgtcgaggaa
      301 tagcggatcc cgggccaagg tccagatatc gcactggatt tcgccggtcg ggatgcgctc
      361 gggatccgcc ctgcgcgcgc cgaagcgatc gccgtagccc aagccctgca ccaggtgcgt
      421 cagctcatgg ccgatggtgt gataggtgac tttgcgcact cgcagtctaa cgattagccg
      481 gtatagctcg tgttttcgat aaatgacggt ggcactgcct aaatgtttgc gtgtatagcc
      541 gatggtgata gcggcatctt tcaattcggg aaaatagccg agcgagcgct gcaccattgc
      601 caaggtgcga cgtgtaatgc gctgcttcat ctgcgctgtg agtctgagcg gcattgctgg
      661 ggatttcgac gcaatcttgc acggcaggtc gttagtgtca taatgccccc ggccagcctt
      721 acggacattt atgccagacg atattaacaa gaacaaggaa aatcagccgt ttcattgtgg
      781 gtacgtcgcg atcatcggtc ggcccaatgt cggcaagtcg accttgctca accacatact
      841 cggtgagaag gttgcaatcg tctcaccgaa gccgcagacc acgcgcaatc gcatcaccgg
      901 tatccgcacc accgaatcat cgcagatcgt gtttctcgac acgccgggaa ttcactccgg
      961 tcattcgctg atgaaccggc gcatggtcga taccgcattg cagaccctcg atgaggtcga
     1021 tggtgtcgtt tggctgctcg atgcgcatga caaaatcaag caggaagagg agcgcatcgc
     1081 cgaaaccttg cggggcgtcg agacgccggt tctgattcta atcaataaga tcgatttagt
     1141 ctccaaggga aaacttttgc cgctgatgga acgctgcgcg caactgttgc ctgacaaaga
     1201 gattgtgccg gtaagcgcgc taaagggtga tggcgtccaa ttggtgctcg gtatgatcga
     1261 taaatggctg ccggaagctc cgcctttttt taccgagggc gaatacaccg atcagacaga
     1321 gcgttttctc gcgtccgaga tcgttcgtga aaaagtgttt ttgctgaccc gggaagaaat
     1381 tccctatggt gtggcggtga ccatcgacga gtttaccgag aagaccgaaa aaaatctcat
     1441 tgtcatcgcg gcgactattc ataccgagcg tgattcgcac aaaggaatcc tgatcggcaa
     1501 gcgcggcgcc atgctcaaag aaattggcca gcaggcacgc gaggaattag agaagatttt
     1561 gggctgccgg attttcctcg aattattcat ccgggtcgat cccaactgga cgcaaaatcc
     1621 caattctctg gcggacatgg gactgtgagg atgatccgct cgttgcccaa attccaaccc
     1681 gccgcgcgcg aactgccgcg taacttgccc atcgtagcca ttgtcggtcg cccgaacgtg
     1741 gggaagtcga cgttttttaa tcgcttggtc ggcgaacggc gcgctattgt cgatgatcta
     1801 cccggcgtga ctcgcgaccg caactacgct gagacggtct gggctggcag aaaattttta
     1861 ctcgtcgata ctggcgggct tgaatcggcg acggccggca aattagaaga gaacgtccag
     1921 gcgcagagtc atttggcggt ggcggaatcc gatgtcatcg tgttcttgct tgacggcaag
     1981 ggcgggctca atccgttgga cgaggaagcg gttggctggt tacgtcaaac cgaaaagcca
     2041 gtattttttg cggtcaacaa actcgactcg cgccaacggg aaaataatct ttacgaattc
     2101 tatgcgctgg ggttagacaa gatttattcg atttccgccg aacatggcct aggtattccc
     2161 gacctgatgg acgatgtggt gcagcatctt gcccccaata tcgaccgcaa ggaagacgat
     2221 gacgggacag tagcagcgcc gctgcgggtt gccatcgtcg ggcgaccgaa tgtcggcaaa
     2281 tcgacgttaa ccaatcggct attgggcttc caacgatcgg tggtcgactc cataccgggc
     2341 accacccggg acgcggtcga cacgcccttc aacttgcgcg gcgaatcttg tgtgctcatc
     2401 gacaccgccg gtatacggcg caaagcgcga atcgacgatc gcattgaacg ttttagcgtg
     2461 agccgctcgc tcaatgccgt cgatcgcggc gatttgattg ttcatattat cgatggaccg
     2521 gagaatgtca cggaccaaga cgcgcagatc ctcagttatg cggtgcagcg tggcaaagca
     2581 atcttgttgg ccgtcaataa gtgggatttg gttcgtgaaa ccggcggcga tcccgataag
     2641 tatcgcgacg aaatcaaata tcgattgtcg ttccttgaat atgtgccgat ctcatttatt
     2701 tccgcggcga caggtcttgg catccgcaag atgctcgaaa ccgcggcccg cgtggtgcaa
     2761 gcgtatcggc gcaaggtgtc gacgtcgtcg ttaaaccagg cgcttcaaga gattgttcag
     2821 gctcacaccg tgccgctgtt tcgcagccgc ccagtgaagt tttactatgg aacccagacg
     2881 agcatagcgc cgccgacatt tacgttcttt gtcaaccagc cggacggcat tttggagagt
     2941 tatcagcgtt acttggtgaa tcaactgcgg cagcggctgg gctttgtcaa cgcgccgatt
     3001 aggctgcatt ttcgcgcccg gcgcgaagca acccaaggcc gacgcaaggg caattctccc
     3061 ggccggtcgc gctcggcata atagctgttg gcgatgattt ttgttccatt aaagcacacc
     3121 ggcaaattgc gattcgctga aaaaagattt gtaaaaacaa aacctttcgg cgcatcggcg
     3181 gatcatctca gctgatcgcc tagccttcgc tagacattta ttttatcatt cgtcacttat
     3241 tgatccgggg ccgttgacaa aacgccgacg actatgattc agccatcgtt attgttctct
     3301 gaaggaatcc gttcgactat gcggatgcac gacgagcttg tgccatggct ggccttgacg
     3361 cgcgtcaaag gcttgggttg tgtgagcttt aaaaagctcg cggaccactt tggcgatcca
     3421 acacaggcat tcgcggcggc gccaagtgaa cttgacgcca tcgatgggct gcaacgcgat
     3481 gccattgagg ggctggtgag tttttctcag tggagcgaag tagacaacga gctcaagcgc
     3541 attcgcagtg ccggcgtcac gctcatacgt tacactgacg gtgcgtatcc cgcgcggctt
     3601 cgtgccatcg ccgacccgcc gccactgctt tatgtcaagg gagctttcgc gccatgcgat
     3661 gagcgggcaa tggcgattgt cggctcgcgg agcgctagtg actatggccg acgcgtggcg
     3721 cgggatctgg cgcgcggcct agcctcgcat ggtttcaccg tggtcagcgg tctggcgcgc
     3781 ggtatcgatg gcatggccca tgagaccgca ttggatttcg gcgcgcgcac catcgcggtt
     3841 ttgggatccg gagtcgaacg ggcttatccg ccggagcatg ataggttgta tcggcgtatt
     3901 gccgagcagg gcgctatcat ttctgaattg cccatcggaa ctaagccgat ggcgtttaat
     3961 tttccggcgc gcaaccgttt gattagtggc ttgtcgttgg gcgtcgttgt ggtcgaagcg
     4021 acggaaaaaa gcggttcgct gatcacggcg tcccttgccc tcgaccaggg gcgcgaagta
     4081 ttcgcggtgc ccggtgaggt cggctcgagc cgcagccgcg gtgcgcaccg gttgattcgc
     4141 caaggtgcaa aattggtgga gggcgtcgat gatataatag aagagatcgc gccgcagctg
     4201 gcacgccatg agggcacgaa cattaaacaa gaagcgcggc aattgccgca aagcagcagc
     4261 gcggctgtgc aaaagatttt tgcaatgctg caagaacgat ccttgcagat cgatgaaatt
     4321 attgaacaca gcgggcttgt gcccgcacaa gtgcttggaa tccttttgga cctggaattg
     4381 cacggctatt taaggcaatt gccgggaaaa gtctatatcg tcgaaagata acgcgagaca
     4441 acaagcgtca ttacttcttt ttatgagcta acgattatgg cagctaagaa cttagttatt
     4501 gtcgaatctc cggccaaggc aaagaccttg gaaaaatatc tagggcggga ctatcaagtg
     4561 aaggcctcgg tcggtcatgt aatcgatctg ccgaagagta agctcggtgt cgacatcaaa
     4621 aaagatttcg cacccgattt cacggtgatt cagggcaaga agaaagtcat cgatgatttg
     4681 aaaaaagccg ccaagggcaa agcaaacatt tatctggcgt cggatccgga tcgagaaggc
     4741 gaagccattg cgtggcatat cgccagccaa gttgtgaaaa atcacaagca tgtccatcgg
     4801 gtcctgatta acgagattac caaaaaagca gtccaggcgg cgattgccaa tcctctcgca
     4861 ttggaccgca acaagtttga tgcccaggtt gcgcgccgtg tgctcgaccg attggttggt
     4921 tataaaatta gtccgctgtt gtggaccaaa gtgcggcgcg gcctaagcgc cgggcgggtt
     4981 cagtcggtgg cggtgcgatt ggtttgcgag cgtgagaaag atatccgtgc cttcgacccg
     5041 gtggagtatt ggtcccttac ggcgactttg gaagggcgct tgccgccgcc attcaaggcc
     5101 aagctcgtcc agtggcaaga ccaaaaggtc gataacaaga agtttcgtct ggaaaacgat
     5161 gcgaccgtcc agtccatcgt caagtccctg gaaggcgcgt catggatcat cggcgaggtc
     5221 gaaaagaaag agcggcgccg ttatcccgcg ccgccgttcg ttaccagtaa attgcaacag
     5281 gaagcatcgc gcaaactcgg ttttcaaccc aagcgcacca tgcaattggc ccagcaactt
     5341 tacgagggcg tcgaactggg caccgaggga tcggtgggct tgatcaccta tatgcgtacc
     5401 gattcgaccc gcatctccga tgacgcgatt gctgccctac gcggttttat ccagggctcg
     5461 tacggcaaga attatgtccc ggggaagccc aatacgtttc gttcaaagaa aggcgcccag
     5521 gacgcgcatg aggctattcg tccgactgct cttgaataca cgcccgacaa agtacgacgt
     5581 tatctgcgcc gcgatatgtt ccagctttat tcgttgatct gggatcgttt cgttgccagt
     5641 cagatggtgc cggcggtgta cgatcaaacc gcttttgaga tccccgtaaa cgaggcggtg
     5701 ttccgcgcca ccggccagca gatcaaattc gacggtttca tgaaagtcta tattgaggga
     5761 cgtgacgaac gggccaccca gaacaacgac gacgaggaag aggaaaccca ggagcttgac
     5821 ggcgtattgc cggatttaca ccagggcgat gcgctcacgc ttcaggcgat ggagccgcgc
     5881 cagcatttca cccaacctcc gccgcggttt acccaggcgt cattgatcaa ggagctcgac
     5941 gaaaagggta tcggccggcc atcgacgtac gctgcgatta tctccaacat tctcgatcgc
     6001 gagtacgttg cgcagaacga aggacggcaa ttggtgccga ccgatttggg atttttggtg
     6061 aacgacctgt tggttgaaag ttttcccgat atcctcaacg tcgagtttac cgccagcatg
     6121 gaggatgagc tcgacaagat cgaggacggc aaggaaaaat ggaccaaggc gatgaagcgg
     6181 ttttatactc cattcgcgcg cgatctcaag aaagccgaga aagagatgcg cgatgtgaaa
     6241 cgccaggagg tccccaccga cattgcctgc gaaaagtgcg gcaagctgat ggtggttaaa
     6301 tgggggcgta acggcgagtt catcgcctgc ccgagttacc ccgagtgcaa gaacaccaag
     6361 aacttcaagc gcgatgacaa aggcgagatc gagatcgccg ctgaagaaga agtcgacgaa
     6421 gcttgcgaca agtgcggcaa gccgatgctc ctgcgcttcg gcaagttcgg caaatttctc
     6481 ggctgcagcg gatatcccga atgcaaaaac atccaaccgc tacaaaagcc ggtggatctc
     6541 ggcatcaaat gccccgagtg cagagagggc aatatcaagg agcgcaaatc acgctggggc
     6601 aaaatgttct acggttgcga cagatacccg gaatgtaaat tcgcgacgtg ggacaaaccg
     6661 atgccgacgc cctgtcccga ctgcgacagt cctatcttag tcgagaaggt gcttaaacgc
     6721 actggccgca cacaccgctg ctataagaag gagtgcggtt actcgattca agtggcggag
     6781 caatagtctg gcgcagtgcc gctcggtccg cagaaagaat tcgaagcgga tcatcggggg
     6841 tgcacctacc gctaaaccga atcggatgtg aagggcgggt taataacctc gctcttttgc
     6901 tttgcgtagt tcaccggttg gctgacgact ctcgaatagg cagattccgc cccagtatct
     6961 ttcaaattcg acggaatcca actcgagtaa gcttttctta ttcaccgcta ataatcgcac
     7021 atgaaaaatt ctgtttgcat catcggcggc ggcctggccg gttcggaagc agcatggcag
     7081 ttggcgcgtc ggggggtagc ggtggaaatt tacgagatgc gcccgcagcg catgaccgag
     7141 gctcattcga ccgcccattt cggcgagcta gtgtgcagca attcgctgcg ctcggattcg
     7201 ttgtcggcac cggccggttt gcttaaagct gagatgcgcc aattcgactc gctggtcatt
     7261 cgtgtcgccg aggcgaaccg agtgccggcg gggtcggctc tggcggtcga tcgcgaacgc
     7321 ttcgcggtcg gtctcacggc ggctgtggaa acgttgccgc gcgtaaaagt cattcgccag
     7381 gaagtcaaaa cgattccaga cgggggcatt gttattctcg ccaccggtcc gttgacttcg
     7441 cccgagctgg cgggggattt aacggccaaa ttgggcgagg aacatttata tttttacgat
     7501 gcgatatcgc ccatcgtcag cgccgagtcg atcgatatga acatcgcgta ccgcgcgtcg
     7561 cgctacgaca agggtggcga cgactatctc aatctgccga tgacgcgaga agagtactac
     7621 cattttattg agggactgct cacggcgaaa cgggtgccga cgcatagctt cgaacgattt
     7681 gtgccgttcg agggctgcat gcccatcgaa gagatggcag atcgcggcaa agacacgctc
     7741 gccttcgggc caatgcgcgc cgtcgggtta atcgatccgc gtaccggcag gcgaccccat
     7801 gcggtggtgc aactgcgcca ggagaatcgc gagcggacgc tctataacat cgtcggcttt
     7861 caaaccaaga tgacctatcc ggagcagcgc cgtgtgttcg ggctgattcc tgggctctcg
     7921 gacgccgact tcgtgcggct cggtagcttg catcgcaata cttttatcaa cgcgccgcag
     7981 cacttggcgc cgacgctgca gtggcgccgg agagaatcgc tgtttttcgc cggccaaatg
     8041 actggcgttg aaggatacat cgaatcggcg gcggtgggca tgctcgccgg catcaacgct
     8101 gcgcgtttac tttcggccct gcccttattg gtgccgccgc ccactacggc tttgggcgca
     8161 ctgttgcgtt atatcaccga tcccgagcgc aagcgtttcc aaccgatgaa tgtaaatttc
     8221 ggattgttgc cgccgctgtc ggtgcggctg cgcggcaaag ccaagaagga aatgcattcg
     8281 caacgcgcgc ttgcggatct ggcgcagtgg ctggaccaaa ctggcgtaga tggcagagcg
     8341 gcagagttcc aggtgccgag taattctgcg atagtagcgg tcccatgagt ccgcgccgaa
     8401 atgccttcat tgatgtcggc actaatacaa ttctttgtct gatcgcggat atcagagaca
     8461 ctgggcgttt tcgcgtgtta gacgatttgg ctgaaatcgc ccggctcggt cagggcgtcg
     8521 accaaaccgg tgtgattagc gccggcggtg aagagcgtgc cctcgcggtg ctcgctcgtt
     8581 accgtgatcg ttgtaaagat ctcggggtgg aagaaataac cgcggtgggc accagcgcat
     8641 tgcgcgacgc cagaaatagc gacctggtca gggcgcgtat ccgggacgct ctcggcataa
     8701 acattcgggt gatttccgga gcggaagagg ctggatattc ttttcgtgcc gtgcagcgtg
     8761 gcttatccct gtcggcccgt gaactgctgg ccatcgatat cggcggcggc agcactgaat
     8821 tcatttttgg caacgaaaat ggcgtatctc gcgcggtgag catcgatatt ggttcggtgc
     8881 gcttgaccga acgctattta cattccgatc cggtggcgag tggcgaggtc accgccatgg
     8941 gtgctgccgt cgaagctcac ctgtcggcat caaccaacga aattatttct caggcaagca
     9001 gcgcgacctt ggtaggcatc gccggaactt ttacgacctt ggcagccatc gagaaaaaac
     9061 tggcgcgcta ttcgcacagc caagttcatg gcagcgttct cgatttaacc gaagtgaagc
     9121 gccaggtggt catgctgcag aaatgttcca tcgccgtgcg caaacagatc gccggcttag
     9181 aaccgaagcg tgccgacgtg atctatgccg gcgcgtatct ggtcgagaaa atcatggaat
     9241 tgttcggtgc caaacaggta gtggtcagcg accaaggcgt gcgttatggg ctgctttacg
     9301 aagcggcaga ttcgctgtaa cagccgcccc ggtgtgaaaa gatctagttt ttcgcttgta
     9361 acgctgaagc gggccacgag ttaaaacgac cgtgaaagga tttgaatgtg atgcaagcgc
     9421 gcgcttgatg atggccatcg tgaaacgtac aatggcagaa acttagaaag aacctctatg
     9481 tcgatcaaaa ccgaaaaaat ttggctggat ggtgaattgg tgcgctggga ggatgccaag
     9541 atccatgtgc tttcccacgc tctgcactac ggcacggggt attttgaagg tattcgttgc
     9601 tatgagctgg cggacgggag ctcggcgata tttcgcctta cggaacatat gcagcgactc
     9661 gtcgactcgg ggaaaatttt aggttttccg ctgccctact cggtt
//
LOCUS       DATLCH010000136         2124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1890835, whole genome shotgun sequence.
ACCESSION   DATLCH010000136 DATLCH010000000
VERSION     DATLCH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890835"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1252
                     /locus_tag="VMZ02_03985"
     CDS             233..1252
                     /locus_tag="VMZ02_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71121.1"
                     /translation="MKFWSWLLLCALSLAATHPVVAEDFYRGKVIRVIVGGSPGGGFD
                     IYTRAMARHMGKHIPGTPSLVVENMTGAGTFIAAKYVHGSAKPDGLSIGMFNGGLILG
                     RVLGMKGIDIDMRELEYLGVPVQDNPVCALSRTSGVTNMEQWASSKTPIKLGGLSPGN
                     STSDVARIIQSALNLPVQLVEGYKGTNEIRLAVDAGELHGGCWAWETLKTAWAKAIPA
                     GDMNVVLQVTGKKLPELPNAPLALDLAKSDEAKTLLRAGAIDPAAVVRVYVTTPRTPK
                     DRLEILRTAFAKTLTDPEFVSEAKKANLDINPLTGEEVKKLVDDLFKLNPATKTKLVS
                     TLIPK"
     gene            1604..>2124
                     /locus_tag="VMZ02_03990"
     CDS             1604..>2124
                     /locus_tag="VMZ02_03990"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71122.1"
                     /translation="MLKPFILRRLALIIAAAALLCSITTNSHAQGPVLKPEKTTLEVA
                     IAATGPLYLPLLLAADAGYFDRRGLKVNLTQLSATASAQALLSGQVDIYQGGTATIHA
                     NVAGSDIIYIGAAVDKSTLVLFGQKGITTLEALRGKAVATTSVGAFGEIAMRKSAKER
                     GMEVGRDIKLLYH"
BASE COUNT          499 a          607 c          589 g          429 t
ORIGIN      
        1 ctctagctga ggccgcgtaa agcttgtccg ctcccaccca aagcaacgtc atcatcaccg
       61 cgatgatagt catgcgtctc atttatgctc ccttcgccga aaaggtttcg ccggcgcaca
      121 acgtacgcga agcagccgac tgccgtcaag ttgccacgcg ttggtgctct tcgcgcaaag
      181 acattgacaa gaacatgaat tccaccataa tcagccaact cggaggatgg cgatgaaatt
      241 ctggtcctgg ttgttgctct gcgctctttc actcgcggca actcatcctg tcgtcgcgga
      301 agatttttat cgtggtaaag tcattcgcgt catcgtcggc ggcagtcccg gcggcggctt
      361 cgacatctac acccgcgcca tggcgcgcca catgggtaag cacattccgg gcacaccgtc
      421 attggttgtc gagaatatga ccggcgcggg aactttcatc gccgccaagt atgtgcacgg
      481 cagcgcaaaa cccgatggcc tgtccatcgg catgttcaac ggcggattga ttctcggccg
      541 ggtcctcggc atgaagggaa tcgacatcga catgcgcgag ttggaatatc tcggggtacc
      601 ggtccaagac aacccggtgt gcgctttgag ccgaaccagc ggcgtcacta acatggaaca
      661 gtgggcatcg tcgaagacgc cgatcaaatt gggcggcttg agtcccggaa acagcaccag
      721 cgatgttgcg cgcatcattc agagcgcact caatttgccg gtgcaactgg tcgagggtta
      781 caaaggcacc aacgagattc gcctcgccgt cgacgctggc gaactgcacg gcggctgctg
      841 ggcatgggag acgttgaaga cggcttgggc caaagcgatt cccgccggcg atatgaacgt
      901 cgtactgcaa gtgaccggta aaaaactacc ggagctgccc aacgcgccgc tcgctctcga
      961 cttggccaag agcgacgagg cgaaaacgct gctccgcgct ggcgccatag atccagcggc
     1021 ggtagtgcgc gtctatgtga caaccccacg cactccgaaa gaccgtttag agattctgcg
     1081 cacagccttt gccaaaaccc taacggatcc tgagtttgtc agtgaagcga aaaaagccaa
     1141 cctcgacatc aacccgctca ccggcgaaga agtcaagaaa ctggtcgacg atcttttcaa
     1201 gttgaatccg gcgacaaaaa caaaactggt cagcaccttg ataccgaaat gacgtatgtg
     1261 ggttggtggg tgacttgtgt tttggaggat acgccaaacc aggattcgga tttggcgccg
     1321 tagagaacgc gataaaagag cttttaatca gcaaccaaca ccgcgaactt ggtgcctcgt
     1381 gcgtaaatgt atgtgcgggt aggatgcggg tggacacaac agaggttccg tcatacggcg
     1441 aaagccggtt cgatccccgc gcctggatgc cggcctgcgc cggcatgacg gttcaatgaa
     1501 atattttcat actctgcaga cgagcgtaag ctcatgaacc tctgtagcga aaatgccttc
     1561 gcatctttat accggacgca tcgatagtct aggagaccgg atcatgttga aacctttcat
     1621 tctacgccga ctcgctctga taattgctgc cgcagcgttg ctctgctcaa taaccacgaa
     1681 ctcgcacgcc cagggccccg tgctgaagcc ggagaaaacc acactagaag tggccatcgc
     1741 cgccactgga ccgctgtatt tgccgttgct gcttgccgca gacgccggct atttcgacag
     1801 gcgtggcttg aaagtgaatc tcacccaatt gagcgccacc gcatcggcgc aggcactctt
     1861 atccggccaa gtcgacattt atcagggcgg cacggcaacg attcatgcca acgtcgccgg
     1921 ttcggacatc atttacatcg gtgcagcggt cgataaaagc acgctggttt tattcggtca
     1981 gaaagggatt acgacgctcg aagcattgcg cggcaaggcc gtagccacga cgtcggtcgg
     2041 cgccttcggc gagatcgcca tgcgcaagtc cgccaaggaa cgcggcatgg aagtcggcag
     2101 ggatatcaaa ttgctctatc acaa
//
LOCUS       DATLCH010000137        11127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_402647, whole genome shotgun sequence.
ACCESSION   DATLCH010000137 DATLCH010000000
VERSION     DATLCH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402647"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /gene="egtD"
                     /locus_tag="VMZ02_03995"
     CDS             complement(<1..1013)
                     /gene="egtD"
                     /locus_tag="VMZ02_03995"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HUR71123.1"
                     /translation="MKQETSRTIFASDEMARWSAEPDGIWSLHGADLDSVPTLSILTT
                     LWDQPRWLEAYHLYDERGSELFEEICELPEYYLTRTENSILEAHASDIIAAAPVECIV
                     ELGAGYSKKTVHLLTEQLRQRGRCHFAPIDVSIAGLLASQSAVRRDFPEVQFHGIHSR
                     YQEGFSAIDKTLPKLFVFLGSTIGNFNPTEFPRFFRALSDAMGPNDFLLVGADRIKDR
                     NILEPAYNDSRGITAAFILNVFRHINRLLDSNFDLDRMRYQSWFNAEWRQIEMYAVAT
                     EDQNINFPSANARFAWKRHERILVEISRKFDPMRLQEQLGFLGLRPVAHYSDPKELFS
                     VLL"
     gene            1224..1991
                     /locus_tag="VMZ02_04000"
     CDS             1224..1991
                     /locus_tag="VMZ02_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR71124.1"
                     /translation="MDRDEIAIEFRDVSFALSSGRTLLSHLNLQVDRGETLVLLGRSG
                     SGKTTTMRLINRLIDPTAGEVIVNGKPTVDWDPIQLRRRIGYVIQEIGLFPHLTIEQN
                     IGVVPRLEGWEPERIRLRARQLLTLIGLDADRFADRYPRELSGGQRQRIGVARALAAD
                     PPVILLDEPFGALDPITRRDIQQEFKNLQQELGKTMVFVTHDIAEAFVLGNRIGLLKD
                     GEMVLLGAPADLLESNHPEARAFAGCFNDGQAIGSPA"
     gene            1988..3547
                     /locus_tag="VMZ02_04005"
     CDS             1988..3547
                     /locus_tag="VMZ02_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HUR71125.1"
                     /translation="MTLFDFFANNRREFVELVAEHLFLVIMSTGIAVLIGVPLGILLT
                     RRPALSKPILGFANIMQTVPSLALFGFLIPLNIYLFQYKIVGGIGARTAIVALVLYSL
                     LPIIRNTFTGISNVDPAIRDAGRGMGMTDRQLLFQVELPLALAVILAGVRVATVICVG
                     TATIAAAIDAGGLGRYIFRGLRANDNILILAGAVPAALIALGADLLLGYFEKTVQLGA
                     GAHAKSKKLLWAAALIVLLFLGGMYFLTGKNAGRIAVGSKDFTEQVILGEIVAQTIEA
                     KTAAQPERRFDLGGNLAHDALVGGEIDLYVEYTGTGLLAILKEKPIADPQEVYRRVQT
                     EYAKRFGVEWTEPLGFNNTFAILVRGDDAKKLKLKTVSDAAKVSARWRAGFGQDFMSR
                     ADGYPGFAKTYGFHFQEIREMDLSLTYRALAEQQVDLIAGNSTDGLIARYGLTQLEDD
                     RHYFPPYDAVPVVRHAALEKYPGLRDALKQLGGILTVDEMRKLNYAVDGEKRQAKDVV
                     REFLNAKGLIR"
     gene            3631..4683
                     /locus_tag="VMZ02_04010"
     CDS             3631..4683
                     /locus_tag="VMZ02_04010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71126.1"
                     /translation="MPKVKGFIDADGHVVEADAELLDFLPAPFKGRADLLSFPFFPTL
                     DGFHRQARRVVDGKGRYIAKGNLQEWREFLDEDEIAWSVLYPTTGLTFGLVRDKEWAC
                     ALATGYNNYLYETFTKPEKRLKGMALIPLQSIPEAIKELRRVVTELGFVGAVLPAVGL
                     RQPFGHEKYFPVYEAAQELGALLAVHAAPTQGIGVDGFDTLIEVRTLSHGFGQMIQMT
                     SMIYGGVYDRFPQLKVAYAEAGCGWVPYLMERLDLEYEHRSAQAPECKVMPSQHLKSG
                     RIFIHCELEDRGIAQIASLLRDDILFCASDFPHEPRSEFRENVERFMAREDVGAATKH
                     KLCFENPKRMYPLALQ"
     gene            4769..5992
                     /locus_tag="VMZ02_04015"
     CDS             4769..5992
                     /locus_tag="VMZ02_04015"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71127.1"
                     /translation="MNDSKHNGAFRALRYPDYFWFWSSYFISNVGSWMQSIAQGWLLF
                     DLTASPLYLGLFSSLRMVLLLSFFVIGGILSDRIDRRKVMLWIQFIAAGTSLLLALLV
                     SNQAIQVWHIFVLGAVTSTTWAFEQPVRQALLPQLVPRDVLVNALALNALTWNGAGLL
                     GPSLVGVSVGTIGIAGCFYVNVVSYLAVVAALLRLRVPANVQDPERGTVLQSLLGGFR
                     YVRAEPVIFNFLAVSAIFNIFGRSYATLLPVFAKDVLRVGASGFGFLAAGPGLGTIFG
                     SLTLASIGRVEAKRWLMVSMLLAFAAVLCGFAVSRDFSAAVALLVAIGALSTVFETLL
                     STSIQLRVEEAFRGRVSGFYGLTGGGLREFGGMQAGFVAEWTSAPVAIVAGAVVLALV
                     GYTFLGARLKRIGSQ"
     gene            6015..7007
                     /locus_tag="VMZ02_04020"
     CDS             6015..7007
                     /locus_tag="VMZ02_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71128.1"
                     /translation="MTKSQYLLMMMALTIGSMSSAWAQKLDHAVLAYGSTGANLTPFW
                     VAREAGLYKHYGVDVDIVFFRGSTIAINALATKDAHFGAFGASSSVLAKLGGVDTVLV
                     ATAAPGLLFYLITKKEIRNASDLKGKKVAISRPGTDSDLGARVAVQRLGLDEKDVSFI
                     SLGTDSERLAAMVQGVVDATVVTVSGYITGQKLGFHSILDLGQARIPYEAASLITTRA
                     LMRDNPDLVMKYVKGFVAGIHYAKSNREPTVKILSKYMRTDDRDVLNASYDYFVSRII
                     PRVPYVSEKGLQAVIDFIRQRNPQTPNVKAADFMDNRYIKELDDSGFIKGLYKG"
     gene            complement(7114..8580)
                     /locus_tag="VMZ02_04025"
     CDS             complement(7114..8580)
                     /locus_tag="VMZ02_04025"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HUR71129.1"
                     /translation="MNKFFSVLALFAAVLGGALAIGVQRFVPRQLDVTPAAPIQIDAD
                     AAAQRLSRAIRHRTVSPIDSASQAEFERFHAFLTESFPTLHQRLARETVNQHSLLYTW
                     QGTRTELKPILLLAHLDVVPADAESSPRWRHPPFAGAVADGYVWGRGALDDKGSLMAS
                     LEAIEWLVKQDYKPERTIYLAFGHDEETGGENGAAKIAAVLTARKVQLEYVLDEGMNV
                     LSGIIDGVTAPVALIGIAEKGYLSIELNANAAGGHSSIPPNDNAIASVTRALQRLEAA
                     PFPASIRGPTRAMFEFLGPEMGWTAKLVLANLWLFEPLVVHQLAGSPLINAILRTTMA
                     PTMFNSGVKDNVLPTHARAVINLRIMPGETTAGALAQVREIIDDPKVKLTPLAIRIEP
                     STATDIESPSFQLLARTIRQITPRSIVAPALLVAATDSRHYAGLTRSVLRFLPITITA
                     ADTDRFHGIDERISVDDYLRCINFYTQLIKNSQSQLLR"
     gene            complement(8892..9908)
                     /locus_tag="VMZ02_04030"
     CDS             complement(8892..9908)
                     /locus_tag="VMZ02_04030"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR71130.1"
                     /translation="MAKNFYEQWLETPNRLQDELEVGRLVATDKDIPWVSTPQDVKVK
                     LMVANHLGFATMGSNVLKSEIPVGWHTGKHRHGEESMHILEGEGFSIINGQRFNWHTS
                     STLQIPFWAEHQHFNTGNVPVLILHGMCFDLEAFLRVARIEQIETCGPNDPSRIAAMP
                     EETSQYYPDGSRAIIHLEQAPTNKEKLPDGESYNPQGAIAATKNQHDFSNYLVVPKNG
                     FRAVSVAITNRWIEPAFHESGRHKHLEAVVYAIDGQGFTDMQGKRIPWQAGDVLYVPP
                     AMWEHQHINENPNSITQIRIGFNIRQWVTSIWPQGFTSTRIYDDEGNPIEAGRIVRKR
                     ERTY"
     gene            complement(9937..10788)
                     /locus_tag="VMZ02_04035"
     CDS             complement(9937..10788)
                     /locus_tag="VMZ02_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR71131.1"
                     /translation="MRASDGHHHLSAGNVIPFEKLSLLFGGSDDTGVVRGSEMVGDRY
                     QDLLQEITAITEYQWRSGTTRGLVKGFMLSGPPGTGKTTLAKRVAYELGRRFEAENEP
                     PVALAFVDGGEISRSRYGESEERIRDIFLHARSGFTAQGQRSVLLFDDVESIFMARGS
                     QHAKEWHFSQDSVFFHSIDDLDTSRATIILTSNRPDLIDEAIRDRFLSYVVDYPDLDT
                     LLQMVQQIPAVEQLSGTQQSTLKKDLIAAVKNGTVRSMRDGQRFAMRHFVAKLLKKDS
                     LAQQIAE"
BASE COUNT         2318 a         2946 c         3268 g         2595 t
ORIGIN      
        1 aacaagagca ccgaaaacaa ttcctttgga tcgctatagt gcgcgaccgg ccgtaagccc
       61 aggaagccca attgctcttg caagcgcatc gggtcgaact tgcggctgat ctcaaccaag
      121 atccgttcgt gccgcttcca agcaaatcga gcattggcac tggggaaatt tatgttctgg
      181 tcttcagtgg ccaccgcgta catttcgatt tgccgccact cggcgttgaa ccatgactga
      241 taccgcatgc ggtctagatc gaagttgctg tcgagcagac ggttgatatg gcgaaacacg
      301 tttaaaataa acgccgcggt gatgccgcgg ctgtcgttgt aagcaggttc taaaatattt
      361 ctgtccttga tccgatcggc accgacgagc agaaaatcgt tgggtcccat agcgtccgac
      421 agagcccgga aaaagcgcgg gaactcggtc ggattgaaat tgccgatggt actgccgaga
      481 aatacgaaaa gctttggtaa ggtcttgtcg atcgcggaaa agccctcttg atagcgcgaa
      541 tgaatgccgt ggaactggac ttccggaaag tctcttctga ccgctgattg agaagcgagc
      601 aagccggcaa tgctgacatc gatgggtgcg aagtgacagc ggccgcgttg gcgcagttgc
      661 tcggtaagca aatgcaccgt cttcttgctg tagccggcgc cgagctcgac gatgcattcc
      721 accggcgcgg cggcgatgat gtcacttgcg tgggcttcaa ggatgctgtt ctcggtgcgg
      781 gtgaggtagt attccgggag ttcacagatc tcttcgaata gttccgagcc gcgctcatcg
      841 tagagatgat aggcttcgag ccagcgcggt tgatcccaga gagtggtcag gatcgacaac
      901 gtcggcactg agtcgagatc cgcgccatgc aacgaccaga taccatcagg ctcggctgac
      961 cagcgtgcca tctcatcaga agcgaatatg gtgcgtgatg tttcttgttt catggccgac
     1021 taaactataa aagaaattaa cagtggatgc gacatcgaga ctaagtgctt tagcccggat
     1081 gtacgcgtta gaacactcct ctcttttgtc cgtcataccg gcggaagccg gtatccaggg
     1141 tttccgcatg gggatggacc ccggtttcca ccggggtgac gaaaatacgg agcggtaagc
     1201 ggaaactagt ttgttcaatt tttatggatc gtgacgagat tgccattgaa tttcgcgacg
     1261 tcagtttcgc actgtcaagc ggtaggacac tgttgtccca tctcaatttg caggtcgacc
     1321 gcggcgagac tctggtgctg ctcggacgga gcggctcggg caagaccacc accatgagat
     1381 tgatcaatcg cttgatcgat cccactgcgg gagaggtgat cgtcaacggc aagccgactg
     1441 tggattggga tccgatccaa ctgcgccggc gcatcggata tgtgattcaa gagatcggct
     1501 tgttcccgca tctaaccatc gaacagaaca tcggtgtggt gccccggctt gaggggtggg
     1561 agccggaacg tatccgccta cgggcgcgac aactcctgac attgatcggt ctcgacgcgg
     1621 atcgtttcgc cgatcgttat ccgcgtgaat tgtccggggg gcaacgccaa cgcattggtg
     1681 tcgcacgcgc actggcagcc gacccgccgg tgattctcct ggacgaacct ttcggcgccc
     1741 tcgatccaat cacccggcgg gatatccagc aagagttcaa aaatttgcag caggaactcg
     1801 gcaagaccat ggtctttgtc acccatgaca tcgccgaagc ctttgtgctc ggcaatcgca
     1861 tcggtctgct aaaagacggc gaaatggtcc tactcggtgc tcccgccgat ctgcttgaat
     1921 ctaatcaccc cgaggcgcgt gcttttgccg ggtgcttcaa cgatggacag gcgattggga
     1981 gcccggcgtg acactgttcg actttttcgc gaacaatcgc cgcgagttcg tcgaacttgt
     2041 tgccgaacat ttatttctgg tgatcatgtc cacgggcatc gcggtgctga tcggcgtgcc
     2101 gctgggcata ctgctgacgc gccggccggc gctgagcaaa ccaattcttg gcttcgccaa
     2161 catcatgcag acggtgccga gtttggcgtt gttcggcttt ctaatcccgc tgaatatcta
     2221 tttgtttcaa tataaaatcg tcggcggaat cggcgcgcgc acggcgatcg tcgccttggt
     2281 gctttattcg ctcttgccga ttatccgtaa cactttcacc ggcatcagca acgtcgatcc
     2341 ggcgatccgc gacgccgggc gcggcatggg catgaccgat cggcagttgc tgtttcaagt
     2401 cgaactgccg ctcgctctcg cggttattct tgccggcgtg cgcgtggcga cggtgatttg
     2461 tgtcggcacg gcgaccatcg ccgcagctat cgacgccggc ggcttgggcc gttatatttt
     2521 tcgcggcttg cgcgccaacg acaatatcct cattctcgcc ggcgccgtgc cggcggcgct
     2581 gatcgcgctc ggcgcggatt tattactggg ctatttcgaa aagaccgttc aattgggcgc
     2641 tggagcacac gccaagtcga aaaaactact gtgggccgct gcgctgatcg ttctattatt
     2701 tctcggcggc atgtacttct taaccggtaa gaatgccggg cgcattgcgg tcggttccaa
     2761 agatttcacc gagcaggtca tcctcggtga gattgtcgcc caaacaatcg aggcgaaaac
     2821 cgctgcgcag ccggaacgcc gcttcgactt gggcggcaat ctcgcccatg acgcgctcgt
     2881 cggcggcgag atcgatcttt atgtcgagta taccggcacg ggtttgttag cgatcctcaa
     2941 ggaaaaaccg atagccgatc ctcaggaagt ctatcgccgc gtgcagaccg aatatgccaa
     3001 acgattcggt gtcgagtgga ccgagccgtt gggattcaac aatacttttg cgatcctcgt
     3061 gcgtggcgat gacgccaaga aacttaagct gaaaaccgtc agcgatgcgg cgaaagtctc
     3121 ggcccggtgg cgcgccggat ttggccagga ctttatgtcg cgcgccgacg gctatccggg
     3181 tttcgcaaaa acctacggct ttcacttcca agaaatccgc gagatggatt tgtcgctgac
     3241 ttatcgcgct ttggccgaac aacaggtcga tttgattgcc ggcaattcca ccgacggatt
     3301 gatcgcgcgc tacggcttaa cgcaactcga ggatgaccgt cattattttc cgccgtacga
     3361 cgctgtgccg gtggtgcgcc acgcggccct ggaaaagtat cccgggttgc gtgacgcgct
     3421 caagcagctc ggcggcattc tcaccgtgga cgagatgcgc aagctgaact acgcggtgga
     3481 cggcgaaaaa cgccaagcga aggacgtcgt cagagagttt ctcaatgcca aggggttgat
     3541 tcgctgaacc ggcggtgcaa atgatgaagg atctgcgctg gtaaatatcc catgatctat
     3601 gccacaaatt cgcagaagga gactatgccg atgcctaaag taaaaggatt tatcgatgcc
     3661 gatggacatg tcgtggaagc cgatgccgag cttctcgatt ttcttcccgc accgttcaaa
     3721 ggccgggccg atttgctgtc gttcccgttt tttccaaccc ttgacgggtt ccatcgccag
     3781 gcccggcgcg tcgtcgatgg gaaagggcgt tatatcgcga aaggtaactt gcaagagtgg
     3841 cgcgagtttc tcgacgaaga tgaaatcgcc tggtcggttc tttacccgac caccggccta
     3901 accttcggac tggtgcgcga taaagagtgg gcttgtgccc tggcaacggg ctataacaac
     3961 tatctttacg agactttcac caagccggag aaacgcttga aaggcatggc gcttatcccg
     4021 ctgcagagta tcccagaagc gatcaaggag ctcagacggg tcgtcacgga gctcggtttc
     4081 gtcggcgctg tcttgcccgc ggtgggtttg cgccaaccgt tcggccatga aaaatatttt
     4141 cctgtgtatg aagcggccca agagttgggc gcgctgctcg ccgtgcatgc cgcgccgacc
     4201 caggggatcg gcgtggatgg gttcgacacg ctcatagaag tgcgcacttt aagccacggc
     4261 ttcggtcaga tgattcaaat gaccagcatg atttatggcg gcgtgtacga ccgctttccg
     4321 caattgaaag tcgcctatgc cgaggccggc tgcggctggg tgccctatct gatggaacgg
     4381 ctggatcttg agtacgagca ccgcagcgcg caagcgcccg aatgcaaggt catgccgtcg
     4441 caacatctga agagcggccg aatctttatt cattgcgaac ttgaggaccg cggcattgcc
     4501 cagatcgcgt ctctcttacg cgatgatatt ctgttttgtg cgagtgattt tccgcacgaa
     4561 ccgcgcagtg agtttcgcga aaacgtagaa cgattcatgg cgcgcgagga cgtcggcgcg
     4621 gcaaccaagc ataagctgtg ttttgagaat ccgaaacgga tgtatccgct ggcgctgcag
     4681 taaatcacgc gtattccaac cgcgaatgac ctagcgccaa ggctcctggg taagcgcgga
     4741 cgattagccg tcagcacgcg cacccttgat gaacgattca aaacataacg gcgcgtttcg
     4801 cgcgcttcgc tatcccgatt acttttggtt ctggtcaagt tatttcattt ccaatgtcgg
     4861 ttcatggatg cagagtatcg cccaaggatg gctgctcttc gacctaacgg cgtcgccgct
     4921 ttacctcggg ctgttcagct cgctgcgcat ggtgctgttg ctgagttttt tcgttattgg
     4981 cggcattctg tcggatcgta tcgatcggcg caaagtgatg ctgtggattc agttcatcgc
     5041 cgcggggacg tccctgctct tggcgctgct ggtgtcgaat caagcgattc aggtctggca
     5101 tatcttcgtg cttggcgcgg tgacgtcgac tacttgggcg ttcgagcagc cagtgcgtca
     5161 ggccttgctg ccgcaactgg tgccgcgcga cgtcctggtt aacgccctcg ctctcaatgc
     5221 gctgacctgg aacggcgccg gcttgcttgg gccgagcttg gtcggagttt ccgtcggtac
     5281 gatcggcatc gccggatgtt tctacgttaa cgtagtcagc tacctggcgg tagtggcggc
     5341 cttgcttcgg ctgcgcgtgc cggccaatgt gcaggatcca gaacgaggca cggttctgca
     5401 gagtttactc ggcggatttc gctacgttcg cgccgagccg gtaatcttca actttctcgc
     5461 ggtctcggcg atcttcaata tatttggccg ttcctatgca acgctgctgc cggtattcgc
     5521 caaggatgtc ttgcgagtcg gcgcttccgg gtttggtttt ctcgctgcgg gtccggggct
     5581 ggggacgatc ttcggttcgc ttaccctagc gagtatagga cgagtagaag ccaagcgctg
     5641 gctcatggtc tcgatgctgc tcgcgttcgc cgcagtgctg tgcggctttg ccgtgagccg
     5701 cgacttttcc gctgccgttg cgcttttggt tgcgatcggc gctttgagca cggtatttga
     5761 gactctacta agcacgtcga tacagttgcg cgttgaagag gcgttccgtg gtcgggtatc
     5821 cggcttctac ggtttgaccg gcggcggctt gcgtgaattt ggcggcatgc aggcgggctt
     5881 tgtcgccgag tggaccagcg cgccggtggc gatcgtcgcc ggtgcggtcg tcttggcgct
     5941 tgtcggttat actttcctcg gtgcccggct caagcgcatc ggctctcagt gaattttcgt
     6001 cggcgaggtt aacgatgacg aagagtcagt acttgctaat gatgatggca ctaacgatcg
     6061 gctcgatgtc gtccgcatgg gcgcaaaaac ttgatcacgc agtcctggct tacggttcta
     6121 ccggcgcaaa tctgacgcct ttttgggttg ctcgcgaagc ggggctgtac aagcactacg
     6181 gcgtcgatgt cgacatcgtt ttttttcgtg gcagtacgat tgcaatcaac gcattggcaa
     6241 ccaaggacgc tcacttcggc gcctttggcg ctagttcttc ggtactcgcc aaattaggcg
     6301 gtgtcgacac ggtgctggtt gcgacggcgg cgccgggctt gctgttttac ctcatcacta
     6361 aaaaagagat tcgcaatgcc agcgatttaa agggtaagaa agtcgccatc agtcgtccgg
     6421 gaacagattc cgatctcggc gcgcgggtcg cggtacagcg gctcgggctc gatgagaagg
     6481 atgtcagctt tatcagtctt ggcaccgact cggagcgttt agctgcaatg gtccagggcg
     6541 tcgtcgatgc gacggtcgtg accgtttcag gttatatcac cggccagaag cttggatttc
     6601 actcgatcct ggatttaggc caagcgagaa ttccttacga agccgcgagc ttgattacga
     6661 cgcgggcttt gatgcgcgac aatccagatt tggtgatgaa atatgtcaaa ggatttgtcg
     6721 ctggaatcca ctacgccaag agcaatcgcg agccgacggt gaaaattctt tcaaagtaca
     6781 tgcgcaccga cgaccgcgat gtgctcaatg ccagctacga ttactttgtt agtcgaatca
     6841 ttccgcgtgt tccgtatgtc agcgagaagg gtctgcaggc agtcatcgac ttcatccgcc
     6901 agcgcaatcc gcagacgccc aacgtcaaag ccgcggactt catggataat cgttacatca
     6961 aggaactcga cgacagcggg tttatcaagg gcctctacaa aggttgattg tcgcggtctg
     7021 ggagcgatcg attcaccaag gtcggccgga tattttccca ccgtgctgcg atgtcgaata
     7081 cttggattgc gtgaagctac tatacggcga gctttaccga agcaattgcg actggctatt
     7141 tttgatcagc tgcgtgtaga aatttatgca gcgcaggtag tcgtcgactg aaattcgttc
     7201 gtcgatgccg tgaaatctat cggtgtccgc tgctgtaatc gtaattggca gaaagcgcag
     7261 gacgctccgg gttagtccgg cgtaatggcg ggagtcggtt gccgcgacca gtagtgctgg
     7321 agcgacgatt gaccggggcg tgatttgacg gatggtccga gccaggagtt gaaagctggg
     7381 actctcgata tcggtcgctg ttgagggttc tatgcgtatc gccaacgggg tgagtttaac
     7441 cttggggtca tcgatgattt cacgcacctg tgccagcgcg ccggccgtgg tttctcccgg
     7501 catgatccgc aagtttatga cggcgcgggc gtgggtcggc agaacgttat ctttgacgcc
     7561 ggagttgaac atggttggcg ccatggtcgt gcggagaatc gcattaatca gtggtgaccc
     7621 ggctaattga tggacgacca gcggctcgaa cagccaaaga ttggctaaca caagtttagc
     7681 ggtccaaccc atttcggggc cgaggaactc aaacatcgcg cgtgtcggac cgcggatact
     7741 agccgggaag ggagcggctt cgagccgctg caaggcccgg gtgacgctgg caatcgcgtt
     7801 atcgttgggt ggtattgagc tgtggccgcc ggcggcattg gcgttcagct caatacttaa
     7861 atagcccttc tcggcaatcc cgatgagcgc caccggcgcg gttacaccgt cgatgatgcc
     7921 gctgagaaca ttcatgcctt cgtcaagaac atattccagc tgcactttgc gcgcggttaa
     7981 gaccgccgca atcttggcgg cgccgttttc gccgccggtt tcctcgtcgt ggccaaaggc
     8041 taggtagatc gtgcgctcgg gtttgtaatc ttgtttgacg agccattcta tcgcttcaag
     8101 acttgccatc aggctgcctt tgtcatccag ggccccgcgc ccccacacgt aaccgtcggc
     8161 gactgcccca gcgaaaggcg ggtggcgcca ccgaggggaa gattcggcgt ccgcgggcac
     8221 tacgtccagg tgggcaagta acaggatggg tttcaattcg gtgcgagtgc cttgccaggt
     8281 gtaaagcagg ctgtgctgat taacggtttc tcgcgccaag cgctgatgca acgtaggaaa
     8341 tgactcggta agaaaagcat gaaagcgctc gaattccgcc tgtgatgccg agtcgatcgg
     8401 cgagacggtc cggtggcgaa tcgcgcggct aagtcgttgt gcagctgcat cggcatcgat
     8461 ttgaatcggc gctgcagggg tgacgtctag ctgacgcggc acaaagcgct gcaccccaat
     8521 agcgagtgcg ccgccaagga cggcggcgaa cagggcgagc acgctaaaaa acttattcat
     8581 gcaggtccgg ggtagcaact ttggtaggtg acggcaagcc gcttgctcag cccgaaaatc
     8641 actaggcaga gcaagggggg cgccggccta tgtgggtcgt gaggtaggca ggatcagcct
     8701 gaccaaggcc aggagatacc tgaggcgaga tctgagcatg atgttgtccg cgctggaagg
     8761 cattatcgaa tgataagcta gtatagtgca gctgtgcacg cgactatgct agggacggct
     8821 caaaaagcta acacagcctg aatttaaaga tggacttaaa cccaggccga tgagaacaac
     8881 ggaactgttt tttagtaggt tcgttcgcgt ttacgcacga tgcggccagc ttcaattggg
     8941 ttgccttcat cgtcatagat gcgcgtgctg gtaaaccctt gcggccagat tgaagtgacc
     9001 cactggcgaa tattgaagcc aatgcgaatc tgggtgatcg aattcgggtt ttcgttgatg
     9061 tgctggtgtt cccacatggc cggcggcaca tagagaacgt cgccagcctg ccaaggaatg
     9121 cgcttgccct gcatgtcggt gaacccttga ccgtcgatcg catagacgac agcctcaaga
     9181 tgtttgtgcc tgccgctctc atggaaagcc ggctcgatcc agcgattggt aatcgccacg
     9241 ctgacggcac ggaagccatt tttgggcacg acgagatagt tggaaaaatc gtgctggttt
     9301 tttgtcgcgg cgatggcgcc ctgcggatta taactctctc cgtcaggcag tttttctttg
     9361 ttcgtcggcg cctgctcaag atggatgatc gcgcggctgc catcggggta atactgcgac
     9421 gtttcctcag gcatggcggc gatccgcgat gggtcattgg ggccgcaggt ctcgatctgt
     9481 tcaatgcgcg cgacacggag gaaggcttcg agatcgaaac acatgccgtg caggattaac
     9541 accggaacat taccggtatt aaaatgttga tgttcagccc aaaatggaat ctgcagggtg
     9601 ctgcttgtat gccagttgaa acgctggcca ttgataatgc taaacccttc accctcgagg
     9661 atgtgcatcg actcttcacc gtggcgatgc ttgccggtat gccagccgac cggaatttcc
     9721 gacttgagca cattgctgcc catggtggca aaaccgagat gattcgcaac catcagcttc
     9781 actttgacat cctggggcgt tgaaacccac ggaatgtctt tatccgtggc taccaggcgg
     9841 ccgacttcaa gttcatcttg gagtctgttt ggcgtttcga gccattgttc ataaaaattc
     9901 ttggccataa cgattccttt tgtctgtgtt aaacgattac tcagcgatct gttgcgccag
     9961 cgaatccttt ttcagcaact tagccacgaa gtggcgcatc gcgaaacgtt gcccgtcgcg
    10021 catgctgcgg acggtgccat tcttcaccgc ggcaatcaga tcttttttta gtgtgctctg
    10081 ctgcgtgccg gacaattgtt caacggccgg tatttgctgg accatctgaa ggagcgtgtc
    10141 gagatcggga tagtcaacga cataactgag gaagcgatca cgaatcgctt catcgatcag
    10201 atcggggcgg ttgctcgtca aaatgatcgt ggcgcgcgac gtatcaagat cgtctatgga
    10261 atgaaagaaa acgctgtcct gagaaaagtg ccactctttg gcatgttgac tgccgcgcgc
    10321 catgaagatg gattccacgt catcgaacag cagcactgag cgctgtccct gcgcggtgaa
    10381 tcccgatctt gcatggagaa aaatatcgcg aattctttct tcgctctcgc catagcggga
    10441 gcgcgaaatc tcaccgccat cgacgaaggc tagtgcgact ggcggctcat tctcggcttc
    10501 aaacctgcga ccgagttcat aggccacccg tttggccagg gtggttttgc cggtgccggg
    10561 aggaccgctg agcatgaatc cttttaccag gcctcgggtc gttccggagc gccattgata
    10621 ttcggtgatg gcggttattt cctgcaggag gtcttgatag cggtcgccga ccatctcgct
    10681 gccgcggacc actccggtgt cgtcgctacc gccaaaaagc aatgacagct tttcgaacgg
    10741 aataacgttt cccgcggata gatgatgatg tccatcgctt gctcgcattt agtagaatct
    10801 cccagctaaa aggacaatcc cagatgctaa tctaacccat taaaatatca agagcttgcg
    10861 acaatgcaag gatttcataa tgagccggtg gatcgttcgc tcgccggtgt tgtctgtgcg
    10921 ctattcagtg gcacgaattt ctccggtgtg aaattcgtta ctggttggag atcatggaaa
    10981 tcaacgagcg ctcttgagca acgtgtgtag atgctaaaag caactaccac attggccact
    11041 tggacggcga ttgttgtcat caagcaacaa aaagtatttc gagcacgact aaaagctgtt
    11101 caggtagaag ggtgaaccgg ccacgcc
//
LOCUS       DATLCH010000138         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9340763, whole genome shotgun sequence.
ACCESSION   DATLCH010000138 DATLCH010000000
VERSION     DATLCH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9340763"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VMZ02_04040"
     CDS             complement(<1..473)
                     /locus_tag="VMZ02_04040"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HUR71132.1"
                     /translation="MNSAVPEPQSSIQKRVRAVILIASVTVVFVTAAAFVTYEAIDFR
                     SRSLRNLSTLTAVIADNSGAPLAFSNKAVAEEILGALRVEPDIEAAILIDDQGNIFAT
                     YPKTLPAALLPRNIEPKGYRFEDAGLVIFEPVRQENKSIGTLYLRASFFSRYQQF"
     gene            complement(466..1095)
                     /locus_tag="VMZ02_04045"
     CDS             complement(466..1095)
                     /locus_tag="VMZ02_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HUR71133.1"
                     /translation="MAPSQENNRRRLGRACADAWLGLRRFLAPPVRGRRVAYAMLLLP
                     VMLTTPASAQVSKEYQLKAVFLWRLAQFTEWPSDAFENPESPIVICVLGDNPFDGALS
                     AAVSGETAHGRRFVVQLLRVIEQAKTCHILYTTSGAARQAKEIATALAGKSVLTVRDG
                     SASASSFETMIVLISEQNKIRMRINLKAAVAARLVLDPRLLRAAEVIGE"
     gene            complement(1073..>2817)
                     /locus_tag="VMZ02_04050"
     CDS             complement(1073..>2817)
                     /locus_tag="VMZ02_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUR71134.1"
                     /translation="SGATSLPQALRLATNLQVAQINSYNWAISARGFNSGTADKLLVL
                     IDGRTVYTPLFSGVFWDVQHVMLEDIDRIEVISGPGAALWGSNAINGVISVITKSARD
                     TQGALLSGGGGSFLRGFGNGRYGGQVGDNFSYRVYGMGFNQGETFLANGSKANDHWHL
                     GQGGFRADLSPTPGAKAMVQGNFYAGGEEIGRPRDLTMDGQNLLGRWTQTLASNQEIS
                     FQLYWDRTHRRNPDIFSEALNTFDIEFQHRFPVGETHNFLWGGGYRFMDDHVVNGPLP
                     NFAFLPGHQTLQLFSGFLQDEITLVPKSLFLTLGAKIEHNSFTGLEVQPNARIAWMPA
                     TKNHTLWAAVSRAVRSPSRIDTELFVPSTPPFFLRGGGNKFKSETVIAYELGYRAELT
                     QEVTGSISTFFNDYDDIRSAEPIAGAPGQFIILNGLRAKTYGIELAMSWQATHWWRLR
                     GGYTYLKKRILFGNSQDLNQGRGEGNDPQNQFLIQSMINLPANLEFDSILRYVDNLNQ
                     LGPLVPSYTSLDLRLGWRPIASWEVSVVGQNLLNGRHPEFGNPTTRQSIPRSIYGAVT
                     WKFLTSSNGAKSGK"
BASE COUNT          631 a          783 c          775 g          628 t
ORIGIN      
        1 aaaaactgtt ggtagcggct gaaaaaagac gcgcgtaaat acaaggtccc gatcgacttg
       61 ttctcttgac gcacaggttc gaatattact agcccggcgt cttcaaaccg gtagcctttc
      121 ggctcgatat tgcgcggcag cagggcggct ggcagggttt tcggataagt ggcaaaaatg
      181 tttccttgat cgtcgattag gatcgccgcc tcgatatccg gctcgactct gagcgcgccg
      241 agaatctcct cggcaactgc tttgttggaa aacgccaagg gtgcgccgct gttgtcggca
      301 atgaccgccg tcagcgtcga caaattgcgc agcgagcgcg agcggaaatc gatcgcctcg
      361 taagtgacga aagcggcagc ggtgacaaaa accacagtga cactggcgat caggatgacc
      421 gcgcgaacgc gcttttgaat cgaactttgc ggttccggca cggcgctatt caccgattac
      481 ctccgcggcg cgcagcagcc gtgggtcgag caccagacgt gctgcaacgg cggctttcag
      541 attgatccgc atgcgaatct tgttttgctc gctgattaac acaatcatcg tttcgaacga
      601 acttgccgat gcactgccgt ctctgaccgt cagcacgctt ttaccggcga gcgccgtggc
      661 aatctccttt gcttggcgcg ccgcgcccga ggtcgtgtag agaatatggc atgtcttagc
      721 ttgttcgata acccgcagca gttggacgac gaatctccgt ccatgcgctg tttcacccga
      781 cacggcggcg cttaaagcgc cgtcgaacgg attatcgccg agcacacaga tgacgatcgg
      841 gctttcggga ttttcaaaag catcgctggg ccattccgtg aactgcgcta agcgccaaag
      901 aaacacggct tttaattggt actctttcga gacttgggca gatgccggcg tggtcaacat
      961 aaccggcaat aaaagcatgg cgtacgcgac ccggcgcccg cgcacgggcg gcgccaggaa
     1021 acggcgcaac ccgagccacg catcggcgca agcgcgcccc aggcggcgcc ggttattttc
     1081 ctgacttggc gccattgctg ctcgtgagga atttccaagt cactgcacca taaatgctcc
     1141 gcgggatact ctgacgcgtc gttggattgc cgaactcagg atgccgccca ttcaataggt
     1201 tctgtcccac gaccgacacc tcccaactgg caatcggtcg ccaacccagg cgcaaatcca
     1261 gagatgtgta gctcggcact aacggtccga gctgatttag gttgtcgacg taacgtagta
     1321 tcgaatcgaa ctctaggttg gcgggcagat taatcatcga ttgaattaga aattgattct
     1381 gtggatcgtt gccctcgccg cgtccttggt tgagatcttg gctattgccg aacaagatgc
     1441 gtttttttaa gtaggtgtaa ccaccgcgca agcgccacca gtgggttgcc tgccaagaca
     1501 tcgccagttc aatgccgtat gtttttgccc gcagaccatt gagaataatg aactgcccgg
     1561 gtgcgcccgc tattggttcg gcgctgcgaa tgtcatcgta gtcattaaaa aaagttgata
     1621 tggacccggt aacttcctgg gtcagctcgg cgcggtaacc gagctcataa gcgataaccg
     1681 tctcggattt aaatttattt ccgccgcctc gaagaaaaaa cggcggcgtg ctaggcacga
     1741 acagttccgt atcaatacgg ctcggtgaac gcactgcccg cgaaaccgca gcccataacg
     1801 tgtgattctt cgtcgccggc atccaggcga tgcgcgcgtt aggctgcacc tccaatccgg
     1861 taaagctgtt gtgctcgatc tttgcgccga gggttagaaa gaggcttttg ggaaccaatg
     1921 taatctcgtc ctgcaagaaa ccgctgaaaa gctgcaatgt ctgatgcccc ggtaaaaagg
     1981 caaaattagg taacggaccg tttaccacgt ggtcgtccat gaaccggtag ccaccgcccc
     2041 aaagaaaatt atgagtttca cctactggaa atcggtgctg gaattcaatg tcaaacgtgt
     2101 tgagtgcttc agaaaaaata tcgggattgc gtcgatgagt tctgtcccag tagagctgga
     2161 acgaaatttc ttggttgctg gccagggttt gcgtccaacg gccaagtagg ttttggccgt
     2221 ccatcgtaag gtcgcgcggc ctgccgatct cctcgccacc agcgtagaaa ttcccttgaa
     2281 ccatggcttt ggcgccggga gtgggggaca agtcggctcg aaacccccct tgccctaaat
     2341 gccaatggtc gtttgccttt gagccattgg ccaagaatgt ttcaccctga ttgaagccca
     2401 tgccatatac acgatagcta aagttgtcgc caacttggcc gccgtagcgg ccattgccga
     2461 agcctctgag aaaagatcca ccgccaccgc tcaacagtgc gccttgagtg tcgcgcgcgc
     2521 ttttggtgat cacactgatg acgccattga tggcattgga gccccagagt gctgcgcccg
     2581 gaccgctgat aacttcgatg cggtcgatgt cttcgagcat cacatgctgc acatcccaga
     2641 acactcccga gaaaagcggt gtatatactg tccgtccatc gatcagcacg agcagtttgt
     2701 cggcggtgcc ggagttaaaa ccgcgcgcgc taatcgccca gttgtaggaa ttgatctgag
     2761 ctacttgaag gttcgtcgct agacgcagcg cctgcggcag gctcgttgct ccggatc
//
LOCUS       DATLCH010000139         8761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4112625, whole genome shotgun sequence.
ACCESSION   DATLCH010000139 DATLCH010000000
VERSION     DATLCH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4112625"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1493
                     /locus_tag="VMZ02_04055"
     CDS             273..1493
                     /locus_tag="VMZ02_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HUR71135.1"
                     /translation="MADLYPYPRFSLAERDRRWKAVRAKMAEQNIDVIVTPQNTGHSM
                     DFQANTRYLTHCGGGGDADIAAVFPLDGAVTAIATSANPRWTTTQEWTTDVREARREY
                     GKIIVERLKELALDHGRIGITGLGEVEGTRTPEGTILHGTWQRIRDAFPNAQLVDATP
                     ILVDVRYIKSAEEIGVLTKSMTIVEEGYKAEIEAAQPGVRDWDVWAAAQYGMMRNGSE
                     MPVHCNWISGKNPVRTLTRPSMRTLEYGDLIINELEANWVGYRAQGVQPVFVGAANPV
                     HQELIKVQREIFNRVMERLKPGVTVGELAELTVKTAAAAAPKSGLAAGARGDLNMHGR
                     GAGDDGPIITAHAKSARQLGVTLQENMVFICKPSAIAADGKSLSTWGDTIVVTATGGK
                     RLGTRPHDLAVSGG"
     gene            complement(1588..3102)
                     /gene="lnt"
                     /locus_tag="VMZ02_04060"
     CDS             complement(1588..3102)
                     /gene="lnt"
                     /locus_tag="VMZ02_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HUR71136.1"
                     /translation="MKFYKILLPILAAALLAAPFLEPWWFPLAWIAFVPLFYVIERAD
                     TLRRAVFYGWLMGLAAHLIGFHWLTYTISVFGGFPFAVSIVVFVLYAALQGIQMALFA
                     LLVRSIGFGPLMIFPAVFWVVCEFLFPLLFPWYIANSQVSFLWVIQTADLVGPYGTGF
                     IVIWFSAALYHALFANVEPRRKIVPLAYACLAIVVALVYGFQRLQSVGDDMAGAGKLS
                     VGAVQGNVDIDMKWNPVLAQKNLAHHRQLTDQLDAVPLVIWPESAIEALIPEAMAALP
                     LDVMPTFKAERAYFIFGAKSFRGTPGKTDFKAFNTAFFADAKGRILARYHKQVLLAFG
                     EYLPFARLLAWIPAMPFADGFTPGPGPAVFHLPRSVRAAPLICYEDLMPELARRFVTE
                     TRANVLINLTNDAWYGKSVGPWQHLRLAQSRAIETRRSLLRVTNTGVTSLVNAKGELV
                     KLLPMFTSAVMQTEVDVLNETTYYVQFGDWFAWLMTIAAGALILFHVKRSWLRK"
     gene            complement(3255..3830)
                     /locus_tag="VMZ02_04065"
     CDS             complement(3255..3830)
                     /locus_tag="VMZ02_04065"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HUR71137.1"
                     /translation="MFAHKISSALISRTPEIIRGDGLKPAAVLIPIQERADGDYVVLT
                     KRGDDMPTHKGQIAFPGGRVHVGDADLIATALRESQEEIGVDPEHVRVLGRLDEFTAG
                     YGIVVTPVVGVIPSQYDFRLDPAETSAVASVPIRSLMAPGTYVKNAHLSPGGHPSYHF
                     YINNGWDVWGVTARILVQFLELAYGFETEKK"
     gene            3910..4566
                     /gene="nth"
                     /locus_tag="VMZ02_04070"
     CDS             3910..4566
                     /gene="nth"
                     /locus_tag="VMZ02_04070"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HUR71138.1"
                     /translation="MTDRELHAAIRILRREVPKWETPVVTLMAETYASPFRVLISCIL
                     SLRTQDATTAKASHRLFAVADTPQAMVKLSTKKIEKLIYPVGFYKTKARQIREICRRI
                     IAQYGGRVPDEIDELLKFNGVGRKTANLVVTLGYNKPGICVDTHVHRISNRWGYINTA
                     TPEKTEIALRAKLPKRYWIEYNNLLVNFGQHLCRPISPQCSVCPVKKYCPQLGVGVRR
                     "
     gene            4568..5806
                     /locus_tag="VMZ02_04075"
     CDS             4568..5806
                     /locus_tag="VMZ02_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71139.1"
                     /translation="MAQRIPLGYFAKNIEPVGYHDLHGKPAFKLAMQEKNDRWYLYVA
                     HLWHRGWSVIDVTDPAAPEFCVYIPGPENTWTIQIQVAEAKMIAGLERIAPGWGGADG
                     EPAAEGFYIFEVSEPTAPKRVGHFETGSTGTHRNFYDGGNIVHAAAGAPGLSGKIYRS
                     IDISDPDHPREVGRFALPEQEMGVDTKGLKFSFHGPAHIEGARAYLSYGDGGGIILDV
                     SDPAKPKLVSQLAFRGITATQGIHTYLPLPRRKIALINDEAIRENGDENLNLAGIVDI
                     SDESRPRMLSLFPLPEPPPDSGLKNFYQKAGRFGPHNHHHANHQPCLEDRDDIVYLTY
                     FNAGLRVYDIRDPRQPKEIGYFVPGDPTKRIGTKPSRLVAQSEDVLVDRRGFIYLSDK
                     THGIHVLRVKQIWPYFKDRS"
     gene            complement(5899..6222)
                     /locus_tag="VMZ02_04080"
     CDS             complement(5899..6222)
                     /locus_tag="VMZ02_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71140.1"
                     /translation="MVHFVIQWDLPDQKANLTIYANKAKNDWLPITLGHKGVIEVRNY
                     RNPLEVTPHVAITIDFDTLDNWRDFIDSADYIRIMRELRVLGCRNFTVNVWAPSKYFP
                     ESMKP"
     gene            complement(6235..7026)
                     /locus_tag="VMZ02_04085"
     CDS             complement(6235..7026)
                     /locus_tag="VMZ02_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR71141.1"
                     /translation="MDLGLKGKVALVAGASQGMGKAAALGFAREGAKVAICARGEAAL
                     KETAERIRKQTGGDVLAIVADMSKAEDIKKFVDDTAQHFGRIDVVVNNAGGPPPGEFM
                     KFTDEDWQNAFNLSFMSTMRMTREAVPYLRKVGGGKVINITSYSVKEPIAGLVLSNAV
                     RSAVIGWAKTLSRELARDNILFNNVCPGRIDTDRAQKLNKARADRLGRSVEEINKEMA
                     AEVPLGRYGTAEEAGDLIVFLGSDRASYITGTTIAIDGGLVRATQ"
     gene            complement(7126..7371)
                     /locus_tag="VMZ02_04090"
     CDS             complement(7126..7371)
                     /locus_tag="VMZ02_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71142.1"
                     /translation="MSCYDVSRLLFDLKMNESIYQRASADLENVMTDYNLSSEEKDAL
                     RAGDPRKLRQLGVHGMLCLYIKRLSPQFRDNIYWAQK"
     gene            complement(7405..8271)
                     /locus_tag="VMZ02_04095"
     CDS             complement(7405..8271)
                     /locus_tag="VMZ02_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71143.1"
                     /translation="MPLVSALAASHAPNILLEPGREWEEFMDLHYSMAPKSSANRPTL
                     EQQKKLRHDAEVAFSTLRADLELAKPDVLIVVANDQFVNFFWNNIPTFFVTLADRVRG
                     QFTRHTFDYQNHKELGKSIVRAGMEKGIDFSYGEHIELQHTQTVPLYFLLPEQTLPIL
                     PIYVNTWVDPAPSPRRCYQVGELIRAVANSAPERVAILATGGLSHFPGSPRIGEIDES
                     FDHELLEIMRNGRGRSLVDYSLQDLLNAGDSEFLNWMVVMGAVGETKAKYTAYMPDFV
                     ATGWGFVSWRLD"
BASE COUNT         2167 a         2504 c         2317 g         1773 t
ORIGIN      
        1 aattcgccgt cgggccctac gacaaaggcc tgtgcgtgca caaacaagaa tggccgccca
       61 aagagttggc ggacgaagcg gagcggcttt tccaagcctc ggtcactcgc atgcgcgcgc
      121 gccgaaaacc gcattgatct gcggcatcga agcccagacg atacattcac ccgacggacg
      181 acggagacga aagcttgccg caatgattct cgaacttcgc gcttgcgtgc ctttcagatg
      241 aaataaccca gtgcattaga cgaaagaaaa atatggccga tctctaccct tatccccgct
      301 tctcactcgc cgaacgcgat cgccgctgga aagccgtgcg cgccaaaatg gccgaacaga
      361 acatcgacgt catcgtcact ccgcaaaaca ccggccattc gatggatttt caagccaaca
      421 ctcgttactt gacccattgc ggcggcggcg gcgatgcgga tattgccgcg gtgtttccgt
      481 tggacggcgc cgttacggcc atcgccactt cggccaatcc gcgctggacg accacgcaag
      541 aatggactac cgacgttcgc gaagcacggc gcgaatacgg caagatcatc gtcgagcggt
      601 tgaaagagtt ggctcttgat catggccgta taggcatcac cggccttggc gaagtcgaag
      661 gcacgcgcac gcccgaaggc acaattctgc acggcacctg gcaaagaatt cgcgatgctt
      721 ttcctaacgc gcaactggtc gacgccacgc cgatcctagt cgatgtgcgc tatatcaaga
      781 gcgccgaaga aatcggcgta ttaaccaagt ctatgacgat cgtcgaggaa ggctacaaag
      841 ccgaaatcga agcggcccaa cccggggtac gggactggga tgtctgggcc gcggcgcaat
      901 acggcatgat gcgcaacggc tcggagatgc cggtgcactg caattggatc tccgggaaaa
      961 accccgtgcg cacgctcacc cgtccgagca tgcgcaccct cgagtacggc gatctgatca
     1021 tcaacgaatt ggaagcgaac tgggtcggtt atcgcgcgca aggcgtgcag ccggtattcg
     1081 tcggcgccgc caatcccgtg caccaagaat tgatcaaagt ccagcgcgag atcttcaacc
     1141 gggtcatgga acgtttaaaa cccggagtaa cggtcgggga attggccgaa ctgacggtga
     1201 aaaccgccgc agctgccgcg ccaaaatccg gcttagccgc gggcgcgcgc ggcgacctga
     1261 acatgcatgg ccgcggtgcc ggcgacgacg gtccaatcat cacggcccat gccaagagcg
     1321 cgcgacaact gggcgtcaca ctgcaagaaa atatggtttt catctgcaaa ccatcggcaa
     1381 tcgccgcaga cggcaaatcg ctcagcacct ggggcgacac catcgtcgtc accgccaccg
     1441 gcggcaaacg tctcggcacc cggccgcatg atttagcggt gtctggcggc tgattcttcg
     1501 gcggcggaga gaaacgaagg ttcggaaaca tgaacctacg ctcggcgttc tgaaagtcga
     1561 ggtagcaaaa ggccgacggc agcttcgtca ttttcttaac cagctgcgct tcacatgaaa
     1621 caaaatcagc gcacctgccg caatcgtcat gagccaggcg aaccagtcgc cgaattggac
     1681 gtagtaagtc gtttcattta agacgtcgac ttcggtctgc atgaccgccg aggtaaacat
     1741 cggcaagagc ttgactagtt cacccttggc gttgactaaa gaggtcacac cagtgttggt
     1801 aacgcgcagc agactgcggc gggtttcgat ggcacgcgac tgggctaggc gcaggtgctg
     1861 ccaaggccca accgatttgc cgtaccaggc atcgttggtg agattgatca aaacattggc
     1921 acgggtttcg gtgacgaacc tgcgcgccaa ttccggcatc aaatcttcgt aacaaatcaa
     1981 tggcgcggcc cggaccgagc gcggcaggtg aaaaaccgcg ggacccggtc caggtgtaaa
     2041 gccatcggca aatggcatag cgggaatcca ggcaagcagc cgggcaaatg gcaaatactc
     2101 gccgaaggca agaagcacct gtttatggta gcgggcgaga attcgtcctt tggcgtcggc
     2161 aaaaaaagcc gtgttaaagg ctttgaagtc ggtcttgcca ggggtacctc gaaaactttt
     2221 agcgccaaaa ataaagtaag cccgctccgc tttgaaagtt ggcatgacat cgagtggcag
     2281 cgctgccatc gcttcgggaa taagcgcttc tatggcggac tccggccaaa tcaccaacgg
     2341 gacggcatct aactgatccg tgagctgacg gtggtgcgca agattttttt gcgccagcac
     2401 cggattccat ttcatatcta tgtcgacatt gccctgcacg gcgccgaccg ataacttgcc
     2461 ggcgccggcc atatcgtcgc cgacgctttg caaacgctgg aacccataca ccagcgcaac
     2521 gacgatcgcc aaacaggcgt aggccaacgg cacgatcttc cgtcgcggct cgacattggc
     2581 gaacagggca tgatacagcg cggcgctgaa ccaaatgacg ataaagccgg taccatatgg
     2641 accgaccaga tcggcagtct ggatcaccca aagaaacgag acttgagaat tggcgatata
     2701 ccaggggaac aagagcggga agagaaattc gcaaaccacc caaaacaccg ccgggaaaat
     2761 catcagcggg ccgaaaccga tgctacgcac gagcagcgca aacagcgcca tctgaatacc
     2821 ttgcagtgcc gcatagagca cgaacaccac gatgctgacg gcgaacggga agccgccgaa
     2881 cacgctgatc gtataggtca gccagtgaaa gccaattaaa tgcgccgcca gccccattag
     2941 ccagccgtag aaaaccgcac gccttagagt gtcggcgcgt tcgatgacat aaaacagcgg
     3001 cacgaaggca atccatgcca gcgggaacca ccacggctcc agaaatggcg cagcgagcag
     3061 cgccgctgcg agaatcggca gtaggatttt gtaaaacttc acggaatcgg attgtcgtta
     3121 ataagtatcg cccgttgcga ctagactaga taagtatctt tgccattaac cgaatgtgta
     3181 aaacgtcagc gcgaacgccc gattgactgc gagaaaataa tgcctgcagc aagttgatga
     3241 gaatcactga gaggctattt tttttcagtt tcaaaaccat aagccaactc caagaactgc
     3301 accaggatgc gcgccgtcac gccccaaaca tcccagccat tattgatata gaagtgataa
     3361 ctcgggtgac cgccgggcga gagatgggcg ttttttacat atgtccccgg cgccatcagc
     3421 gaacgaatcg gcactgaggc caccgcgctg gtctccgccg gatcgagacg aaaatcgtat
     3481 tgcgacggaa tcacgccgac aacaggcgta acaacgatgc cgtaccctgc ggtgaattcg
     3541 tctaaacggc cgagcactcg tacatgttcc ggatcgacgc cgatctcttc ctggctctcg
     3601 cgcagcgcag tggcaatcaa atcggcgtcg cccacatgca ctcgccctcc gggaaaggcg
     3661 atctgcccct tatgcgtcgg catgtcgtcg ccccgtttgg tcaggaccac ataatcgccg
     3721 tccgcgcgtt cttggatcgg aatcaagacc gccgcaggtt ttagaccgtc acctctgata
     3781 atttccggcg tgcgagaaat cagagcggag gaaatcttgt gagcgaacat gaacaagtaa
     3841 cttcgtggcg atcgcgatct gtgatagata ccgttgcaat ttagccaatc gaagggcaaa
     3901 acactagccg tgaccgatcg agaacttcat gcagcgatcc gcatcctgcg ccgcgaagta
     3961 ccgaaatggg aaacccccgt agtaacactg atggcggaga cctacgccag tccatttcgc
     4021 gttttgatat cttgtattct cagtttgcgc acccaggacg cgaccacagc caaagcgtcc
     4081 catcgcctat tcgccgtcgc tgacacgccg caagcgatgg tgaaactctc gacgaagaaa
     4141 attgaaaagc tgatttaccc ggtcggcttc tacaagacta aagcgcggca aattcgcgag
     4201 atttgccgaa ggatcatcgc tcagtatggt ggcagagtgc ccgatgagat cgacgagctg
     4261 ctcaaattca acggcgttgg gcggaaaaca gcgaacctgg ttgtcactct gggatacaac
     4321 aagccgggca tctgcgtcga cacccacgtg catcggattt ccaaccggtg gggctatatc
     4381 aacaccgcga cgccggagaa aaccgaaatc gccttgcgcg ccaaactgcc caaacgttat
     4441 tggatcgagt acaacaattt gctggtgaat ttcggccagc atctgtgccg gcccatctcg
     4501 ccacagtgca gcgtatgtcc cgtcaagaaa tattgcccgc aactcggcgt tggcgtgaga
     4561 cgataacatg gcccaacgaa tccccttagg ctacttcgcc aagaatatcg aaccggtcgg
     4621 ataccatgat ctgcacggca agcccgcctt caagctggcg atgcaagaaa agaatgatcg
     4681 ctggtacttg tacgtcgccc acctttggca ccgcggctgg agtgtgatcg acgttaccga
     4741 tccggcagcg ccggaatttt gcgtttatat tcccggcccg gaaaatacct ggacgattca
     4801 gattcaagtt gccgaggcta aaatgatagc cggccttgaa cgcattgctc ccggctgggg
     4861 cggcgccgac ggggaaccag cggcggaagg tttctatatc ttcgaagtga gcgaaccgac
     4921 cgcgcccaag cgggtcggac attttgaaac cggcagcacc ggaacgcatc gtaattttta
     4981 cgacggcggc aacatcgtcc acgccgccgc cggtgcacct gggctgagcg ggaaaattta
     5041 ccgtagtatc gacatcagtg atcccgatca tccgcgcgaa gttggccgat tcgctttgcc
     5101 cgaacaggaa atgggtgtcg atacgaaggg attaaaattt tcgtttcatg gaccggcgca
     5161 tattgaaggc gcgcgcgctt atctctccta tggtgatggc ggcggcatta ttctcgacgt
     5221 cagcgatccg gcgaagccaa agctagtgag tcaactcgcc ttccgtggca ttactgcgac
     5281 ccaaggaatt catacgtatc tgccattacc ccgacgaaag atcgcgttga tcaacgacga
     5341 ggcaattcgt gaaaacggtg atgagaattt gaatctcgcc ggcatcgtcg atatcagcga
     5401 tgaaagcaga ccgcgcatgc tgtcgctttt tcctctgcct gaaccgccgc cagacagtgg
     5461 gttaaagaat ttttaccaga aggcgggccg ctttggaccg cacaaccatc atcacgccaa
     5521 tcatcagccc tgcctcgaag atcgcgacga tatagtctat ctcacctact tcaacgctgg
     5581 tttgcgagtc tacgatattc gcgatccacg ccagcccaaa gaaatcggct acttcgttcc
     5641 aggagatccc acgaagagga tcggcaccaa gccgagtcgg ctagtggccc agtccgaaga
     5701 cgtcttagtc gaccggcgcg gattcatcta ccttagtgac aagacccatg gcattcacgt
     5761 tttgcgtgtc aaacaaattt ggccgtactt taaggatcgc tcatgaggca tgcgcgcagt
     5821 ggaacttaat catggcgcaa tcaacgtaac aattatgggg ttagacggct ttaaattttg
     5881 aactcttgag tgacttagct aaggcttcat cgattcagga aagtatttcg aaggcgccca
     5941 aacgttgaca gtgaaatttc tgcagcccaa gacgcgcagc tcgcgcatga ttcggatgta
     6001 atccgctgag tcgataaagt cgcgccaatt atccagcgtg tcgaaatcga tggtaatcgc
     6061 cacatgcggc gtcacttcga gcgggttgcg ataattgcgc acttcgatga cgcccttatg
     6121 tcccagcgtg atcggcaacc agtcgttctt cgccttgttg gcgtagatcg ttaaattggc
     6181 tttttgatcg ggtagatccc attggatgac gaagtgtacc atggatgtat cctcttactg
     6241 ggtagcccga actaagccac cgtcgatagc gatcgtcgtc ccggtgatgt aactcgcccg
     6301 atccgagccg agaaacacga tcaaatcgcc ggcttcttcc gccgtcccgt agcgtcccaa
     6361 cggcacctcc gccgccattt ccttgttgat ctcttccacc gaccggccca gacgatcggc
     6421 gcgcgcctta ttcaattttt gcgcgcggtc ggtatcgatg cgcccagggc acacgttatt
     6481 gaacaagata ttgtcgcgcg ccaattcgcg tgacagggtc ttggcccagc cgataacagc
     6541 acttcggaca gcattggaca gcaccaaacc ggcgatcggt tccttgacgg agtaagaagt
     6601 gatgttgatt actttcccgc cgccaacttt gcgcaaatag ggcaccgctt cgcgagtcat
     6661 gcgcatggtg ctcatgaagc ttaaattaaa tgcgttctgc cagtcctcat cggtaaactt
     6721 catgaactcg ccgggaggcg ggccgccggc gttattaacc acgacatcga tacggccgaa
     6781 atgttgcgca gtgtcgtcaa cgaatttctt aatatcttcc gccttactca tgtcagcgac
     6841 gatcgccaac acgtcgccgc cagtctgctt acgaattcgc tctgccgttt ccttcaacgc
     6901 ggcttccccg cgggcgcaga tcgccacctt ggcgccttcg cgcgcgaaac cgagcgccgc
     6961 cgctttgccc atgccctgac tggcaccagc gaccagcgct actttacctt tgagtccaag
     7021 atccatgtat acttttcccc ctgatagatt tgagtgtgcc ggatactaac aaacccactc
     7081 tcgtgggtca aaattgactt cgaggcgagg cgatcgaaat agctgtcact tctgggccca
     7141 atatatattg tcgcggaact gcggactcag ccgcttgatg taaagacaga gcatgccgtg
     7201 gacacccagc tggcgcagct tgcgcggatc gcccgcgcgc agcgcgtctt tttcctcgct
     7261 cgacagatta taatcggtca tgacgttttc taagtcggct gatgctcgct ggtagatcga
     7321 ttcgttcatc ttcaggtcga acaacaggcg gcttacatca taacaactca caaatcctcc
     7381 agaacgcgtg aaagcaaacc cactttaatc caatcgccag ctgacaaaac cccagccggt
     7441 agcgacaaag tccggcatgt aagcggtata cttcgccttg gtttcaccaa ccgcgcccat
     7501 caccaccatc cagtttagaa actcgctgtc gccggcgttt aaaagatctt gaagcgagta
     7561 atcaaccaaa ctccgtcccc gaccgttgcg catgatttca agcagctcat gatcgaaact
     7621 ctcgtcgatt tcgccaatac gcggcgaacc ggggaagtgc gacagtcctc cggtggcgag
     7681 gattgccacg cgctccggtg cgctattcgc cactgcacgg atgagctcac ccacttggta
     7741 acagcgccgc ggcgaaggag cggggtcaac ccaggtgttc acgtagatgg gcaagatcgg
     7801 cagcgtctgc tctggcagaa gaaaataaag cggcaccgtt tgcgtatgtt gcaactcgat
     7861 atgctccccg tacgaaaaat cgatgccctt ttccataccg gcgcgaacaa tcgatttgcc
     7921 gagctccttg tgattctgat aatcaaatgt gtgtcgggtg aattgccccc tcacccgatc
     7981 ggcaagggtg acaaaaaagg tcggaatatt gttccagaaa aagttaacaa actgatcgtt
     8041 ggcgacaacg atcagcacat ccggcttggc gagctctaga tcggcgcgca acgtagagaa
     8101 tgcgacttcc gcgtcatgcc gcagcttctt ctgctgctct agagtcggcc gattcgccga
     8161 gctttttggc gccatgctgt agtgcaaatc catgaactct tcccattccc ttccgggctc
     8221 gagaagaatg tttggcgcat gactcgccgc caacgcggat actaatggca tagctcaaac
     8281 tcctccggtg aagtgagcga aatcctatgc cgcgacctgg gaaaatgcaa ctatgaaacg
     8341 gcagttacaa aacgagagtg atggaaggga aaagtaagtc gaacaccggc gaagatagag
     8401 cccttgatgt aaaatagatc agcggactgt cactgatcta aagcatgcga tagttccgca
     8461 ctgaagccca gaccgctaca aagataacga ctaatgaaac aatcgggtga ttcaacaccc
     8521 agactaacgc cggctcaaac gcatgccgcg aaatccattc aaaagaccgt gacagttgat
     8581 ccatgcctca cctcattaat tattcgtcga catcttaata caactggagc caaccggaaa
     8641 ctacatttcg gcagattcta ttcattcaag ctgtagcttc ttcgcacact gactctggtc
     8701 agcaatgctc acgggtccgg cgatcatttc tgcacagagt cgaaggccgc tttaactgca
     8761 c
//
LOCUS       DATLCH010000140         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7654694, whole genome shotgun sequence.
ACCESSION   DATLCH010000140 DATLCH010000000
VERSION     DATLCH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7654694"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..683)
                     /locus_tag="VMZ02_04100"
     CDS             complement(150..683)
                     /locus_tag="VMZ02_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71144.1"
                     /translation="MSIVTQLAGFRAQQFVALILDNEVTVGEFVTEPPLSWARIVQQG
                     GVFQIAQGYPKVLDAALGRAEMTNWDEVSLPGVGRTLAELADAVDYVVIGNNAGQGLP
                     LAQAVPQKLRATQSGIIYARSLPEQSAYERLGYQNFFRRYETSARLLAAAEAKNRPLA
                     LYFMNTIQHNKFNYHDP"
     gene            complement(738..1733)
                     /locus_tag="VMZ02_04105"
     CDS             complement(738..1733)
                     /locus_tag="VMZ02_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71145.1"
                     /translation="MISQKFMPFMVIAFFFSCLEAVHALDKIRIGMGAFSPSNGAIWV
                     AEERGFFKKHGLDVEAIFIGGGAARGINALLAGDIQFASASGGPVISSMLKGIDVVMI
                     AALNNKGVHRLMVRPDIDTPEALKGKRIAITAFGTASHTVLTLLLNHWNLRQEDMNVL
                     QIGSSPTMLVSLQKKVVDGAVLTDPSYFIAEDNGFRVLADLAKMNIHYLQSMLVSTRG
                     YLRGNRDEATRVMRAYVEGIAYFKRNKKESIRIILKKMRMEADKEKYAERTYDQYANT
                     YFDRVPYPSLSGVRTILESLAKEFPKAKGADPASFSDPSILKSIEDSGFIKSLYD"
     gene            complement(2181..3170)
                     /locus_tag="VMZ02_04110"
     CDS             complement(2181..3170)
                     /locus_tag="VMZ02_04110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR71146.1"
                     /translation="MASKLKFGLLLPHFGSHMSVENCREGARRAEAYGFDSVWVRDHL
                     VFTPHAGEGADANHLECLLLLASLAPLTTRLQFGTAMAICHRHPIHLAQCCAGLSAIS
                     NGRVILGLGLGGFPHEFAAAGRPSSLADRAELARINIEICRALWRGETLSHRSDYFEF
                     TAVALKPLPKNAIPIWIGGSSPAACRRAAALGDGWMPARINYPTFSKRVEYLHRLCAQ
                     AGRPLIDIAVMPFTTIGNNLADALRGINTKDLLDEAHNSSTWVKPPSGKFETLEDLGG
                     VLIAGSPADVVRESQTYEAAGANHIVYDLRLRYTDWHEQIDLLGKEVLPALRS"
     gene            complement(3231..>3715)
                     /locus_tag="VMZ02_04115"
     CDS             complement(3231..>3715)
                     /locus_tag="VMZ02_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71147.1"
                     /translation="MLGACSYPITMQSREGVTYDGRYRFGRDDSGLMQIYGPDNEVLI
                     GHFARVGRTAFVERYEKAFGRGTIELDGPDLSRHAGSLSGVLGPTSGFHETAYADPVA
                     AAQGRPTKSITGPLFYWVASLEGDRRTVLGCFFIGSAYTGGGFGRCKSQAGQEYSIDF
                     "
BASE COUNT          851 a         1054 c         1025 g          785 t
ORIGIN      
        1 gaataatggc ttcatcgggt gagttcactc ggttcattca tactgagata ttagcctaat
       61 cctaaggtgg gataaactcg cagcgggcga tcccgttggt ggaacctttt gtttaggctt
      121 tgggaaggag cgagaaccgg ataatgcttc taaggatcgt gatagttgaa cttgttgtgc
      181 tgaatggtgt tcataaaata aagcgccaac ggccgattct tggcttctgc ggcagcgagc
      241 aagcgtgctg aggtttcgta acggcgaaaa aagttctgat aacccaagcg ctcgtacgca
      301 ctctgctccg gcagactcct agcatagata attcccgatt gtgtcgcgcg tagtttttgc
      361 ggcaccgctt gtgccagcgg caagccctgg ccggcattgt taccgataac gacataatcg
      421 actgcatcag cgagctccgc gagagttctg cccacgccag gcagcgatac ctcatcccaa
      481 tttgtcattt cggctctacc gagggccgcg tccaacacct tggggtagcc ttgggcgatc
      541 tggaagacgc cgccctgctg cacgatgcgc gcccacgata gcgggggttc cgtgacgaat
      601 tcacctaccg tgacttcgtt atcgagaatc agcgcgacga attgctgcgc acgaaaaccc
      661 gcaagctggg tgacaatgct cattagaaat ccttctcctg caccagtact agacatctcc
      721 ggcgcgacaa cagttggtta atcgtaaaga ctcttgatga aaccgctatc ttcgatggac
      781 ttgaggatgc tcgggtcgct gaacgatgcg ggatcggcgc ccttggcctt gggaaactct
      841 ttggcgaggg attcgaggat ggtgcggaca ccgctcaaag acggatacgg gacccgatca
      901 aagtacgtgt tggcgtactg gtcgtaggtg cgctcggcgt atttctcttt gtccgcttcc
      961 atgcgcattt tcttcaagat aatccgaatg ctttctttct tgttgcgttt gaaataagcg
     1021 atgccttcga cataggcacg catcacccgg gtagcctcgt cgcgattacc gcgcaaatag
     1081 ccgcgcgtcg aaaccagcat actttgcaaa tagtgaatat tcatcttcgc cagatccgcc
     1141 aagactcgaa aaccattgtc ctcggcgatg aaataagacg gatcggtcaa caccgcaccg
     1201 tcgacgacct ttttttgcaa gctcaccaac atggtcggcg acgaaccgat ctgcaaaacg
     1261 ttcatatcct cttggcgcag gttccagtga ttgagtaaaa gcgtcaaaac ggtatgcgat
     1321 gcggtgccga aggcggtaat agcgatgcgc ttgcccttca aggcctccgg cgtatcgata
     1381 tccggccgca ccatcaaccg gtgcacgcct ttattgttta gtgccgcgat catcaccaca
     1441 tcgatgccct tgagcataga gctaatgacc ggcccgccgc tagcgctggc gaactgaata
     1501 tcgccggcca gcagggcgtt gattccgcgc gccgccccgc cgccgataaa gatcgcctcg
     1561 acatcaagac catgcttctt gaaaaacccg cgctcctcgg caacccagat cgcgccatta
     1621 ctcgggctaa acgcgcccat gccgatgcgg atcttatcca gcgcatggac cgcttcgaga
     1681 caagaaaaaa agaatgctat caccatgaaa ggcatgaatt tttgagaaat catttgtcgc
     1741 aacgttcgtg tccttctcgg aaaatagatg tgcaggcgcg accgaaggtt gatttggacc
     1801 gagattccgt cataccggcg aaagccggta tccatcccct gcctccctgg atgccggcct
     1861 ccgccggcat gactactaaa ggccaagtcg ccggcccggg gctgacgaat ccgatattaa
     1921 gagtgccggt tgtcacccgt gaatttgtcg catccgtaaa tcaatagcac agcgggttaa
     1981 gaaaaccacg ttagctgtcg actggtattg gcgttagcga cccggaccga cactcacgta
     2041 tgacgatgac caagccaatc gcgacagcgg aacatcgcgg aataaagcta aacggcgctc
     2101 cttgcggtac tggcgcctgg cggcaagaaa tagaattgcg gggagtaatt aactgggggt
     2161 tgatttcgca ctgccgcgtc ttacgaacgc agcgcgggca ggacttcttt acccagcaaa
     2221 tcgatctgct cgtgccagtc ggtgtagcgc aatcgtaaat cgtagacgat atgattggca
     2281 cctgccgctt cataggtttg gctttcgcgc accacgtcgg ccggcgaacc ggcgatcagc
     2341 acgccgccga gatcttcgag ggtttcaaat tttcccgacg gcggctttac ccaagttgac
     2401 gaattgtgcg cttcgtccaa caaatctttg gtgttgatac cgcgcagcgc gtcagcgagg
     2461 ttattgccga tggtggtgaa cggcatcact gcgatatcga tcagcggacg gccggcttgg
     2521 gcgcacagcc ggtgcagata ctccaccctt ttgctaaacg tcggatagtt gatacgcgcc
     2581 ggcatccagc catcgccgag tgcggcggcg cgccggcatg ccgccggcga actgccgccg
     2641 atccagatgg ggatcgcgtt cttgggtagt ggtttcaacg ctacggctgt gaattcgaaa
     2701 tagtcactcc gatgcgacaa ggtttcaccg cgccacagag cgcgacagat ttcgatattg
     2761 atccgcgcga gctcagcgcg atcggcaagc gaactcggcc ggccggctgc ggcaaactcg
     2821 tgcggaaatc cacctaaccc gagacccaga ataacccgcc cgttggaaat cgcactcaaa
     2881 ccggcgcaac attgcgccag gtgaatcgga tggcgatggc agatagccat cgccgtaccg
     2941 aattgcagcc tcgtcgtcaa cggtgccaat gacgcgagca acagcaagca ttccaaatga
     3001 ttggcatcgg cgccttcgcc ggcatgcggc gtgaaaacca agtggtcgcg cacccatacc
     3061 gagtcgaaac cgtaagcttc ggcgcgacgc gctccctcgc ggcagttctc aaccgacatg
     3121 tggctgccaa aatgaggtaa caggagacca aacttgagct ttgaggccat aaatttccag
     3181 cgtgatgttg ccgacggagt cgcgcacgga ggatttgcag gaaacctaga tcagaaatcg
     3241 atgctatatt cttgaccggc ctggctttta catcggccga aaccgccgcc ggtataggcc
     3301 gaaccgatga aaaagcaacc gagcacggtg cgccggtcac cttccagcga agcgacccag
     3361 taaaatagcg gtccggtgat ggactttgtc ggcctaccct gagctgccgc cactggatca
     3421 gcgtaggcag tttcatgaaa acccgacgtc ggtcccaaaa caccgctcag ggatccggcg
     3481 tggcgcgaca agtcgggccc gtcgagctct atcgtgccgc ggccgaatgc tttttcgtac
     3541 ctctcgacaa aagccgtacg gccgacgcgg gcgaaatggc cgatcagcac ttcgttgtca
     3601 ggtccataga tttgcatgag accgctgtcg tcgcggccaa aacgatagcg gccgtcgtaa
     3661 gtcactcctt cgcgtgattg catcgtgatt gggtaggagc aggccccgag catta
//
LOCUS       DATLCH010000141         2702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1154651, whole genome shotgun sequence.
ACCESSION   DATLCH010000141 DATLCH010000000
VERSION     DATLCH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154651"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1892
                     /locus_tag="VMZ02_04120"
     CDS             <1..1892
                     /locus_tag="VMZ02_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR71148.1"
                     /translation="AAAVFLGGRLARSLARLAHSAGDIQRGEPIELRRSAVYEIDELH
                     QQLIQAGALARQNFHERTLRIEAEARRAAAEVARSEILEREAKLQESEERLRAFLENS
                     ATIAWMVDEDGRYVYLSRNYLKRFKLQTQHWQNKTVFDLWPAPIAEKLRRNDLAVLAG
                     GGLQEEMEATTNPDGSRSWWFNSRFVLTDSSGKRYVGSLAVDITERKRLENELESRIA
                     ELKQADKRKDEFLAMLSHELRNPLAPISNAVQILKTLAPNDSKLDWCSNLIDQQVKYM
                     ARLMEDLLDVSRITRDKLDLRKQPVELGEIIGNAVETNRPLIENAGHRLTVDLPQSKI
                     VLDGDPARLTQVFANLLNNAAKYMDGNGEICITARLAGMSALDGDEAEGGPRNRRTEH
                     TAPTSARSKISNPVSAIDEIIVSVADTGIGIAPDKLPHVFDMFYQAEDGRDRRYGGLG
                     IGLTLVRSMVELHGGSIEAKSAGVGQGSEFVVRLPVLHLDLPGIQDSAEPQGVPTRAA
                     TDKRVVIVDDNKMQAQTLAMLLETMGFQVRTAHDGARALDVIAAFVPQVALIDIGLPG
                     MSGYDLAGRLRELPQLNGVTLIAQTGWGREEDREESIRVGFQHHLTKPIDHRLLEKIL
                     RGIVD"
     gene            2234..2605
                     /locus_tag="VMZ02_04125"
     CDS             2234..2605
                     /locus_tag="VMZ02_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR71149.1"
                     /translation="MAKDKRVAIVEDNKLQAQSLAILLEMMGYQVRTACDASTAMSMM
                     AEFVPQVALIDIGLPGLNGYELARRLRDLPQLRDITLIAQTGWGTEDDREQARQAGFQ
                     HHLTKPIDHKRLEEILSQATQ"
BASE COUNT          630 a          726 c          808 g          538 t
ORIGIN      
        1 tggcggcggc ggtatttttg ggtggccgac tggcgcgctc gctcgccagg ctggcgcatt
       61 cggcgggaga cattcagcgc ggcgagccga tcgaactccg acgttctgcg gtctatgaaa
      121 tcgacgaatt acatcagcag ttgattcagg cgggagcgct agcgcgacaa aattttcacg
      181 aacgcacatt gcgcatcgag gccgaagccc ggcgcgctgc cgctgaagtt gcgcgcagtg
      241 agattctcga gcgtgaggca aaattgcagg agagcgagga gcgcttgcgt gcctttctgg
      301 aaaacagcgc gaccatcgcc tggatggtcg atgaggacgg acgttatgtt tacctaagcc
      361 gcaactatct caaacgtttc aaactgcaga cgcaacactg gcaaaacaaa actgtcttcg
      421 atttatggcc ggcgccgatc gcggagaagt tgcggcgcaa cgatttggcg gtacttgccg
      481 ggggcggact gcaggaggaa atggaagcga cgacaaatcc cgatggcagc cgctcttggt
      541 ggttcaattc acggttcgtg ctgaccgata gctcgggcaa gcgctatgtg ggcagtctgg
      601 cggtcgatat caccgagcgc aagcgtctgg aaaacgaact tgaaagccgt attgccgaac
      661 tcaaacaagc cgacaagcgt aaagatgaat ttctcgcgat gttgtcgcac gaactgagaa
      721 atccactggc gccgattagc aatgccgtgc agattctgaa aacccttgcg cccaacgatt
      781 caaagctcga ttggtgctcc aacttaatcg atcaacaggt caaatatatg gcgcgcctga
      841 tggaagatct gctcgatgtc agccggatca cgcgggataa gctcgacctg cgtaaacagc
      901 cggtggagct tggcgagatc atcggcaacg cagtggaaac caaccggccg ttgatcgaaa
      961 acgcggggca ccgtcttacg gtggatttgc cgcaatcaaa gattgtcctt gacggcgacc
     1021 cggcccgact tactcaagta tttgccaact tgttaaataa cgcggccaag tacatggacg
     1081 gtaacggcga gatctgcatc accgcgagat tagccgggat gtccgcgcta gacggtgacg
     1141 aggctgaagg cggcccacgc aatcgccgga cggagcacac ggctccaacg agcgctcggt
     1201 ccaaaatttc caacccggta tctgcaatcg acgaaataat tgtttcggtc gcagacaccg
     1261 gcatcggcat cgcgccggac aaactgccgc atgtattcga catgttttac caggccgagg
     1321 acggtcggga tcggcgctac ggcggcctcg gtatcggcct gacgctagtt cgcagcatgg
     1381 tcgaattgca cgggggcagc atcgaagcaa aaagcgcggg ggttggccag ggcagcgagt
     1441 tcgtcgtccg tttgccggtg ctgcacctcg atctgcctgg catccaggat agcgctgagc
     1501 cgcagggtgt gccgacaagg gcagcgactg acaaacgcgt ggtcatcgtc gatgacaaca
     1561 aaatgcaagc gcagacattg gccatgctcc tcgaaaccat ggggtttcag gtgcgcaccg
     1621 cgcatgacgg cgctcgcgct ctcgatgtga ttgccgcgtt tgttccacag gtagcgctca
     1681 tcgacatcgg gttacctggc atgagcggtt acgatttggc cggccggctc cgtgagctac
     1741 cacaattgaa tggcgtcacc ttgatcgctc agaccggctg gggacgtgaa gaagaccgcg
     1801 aagaatcaat tcgcgttggc ttccagcacc atctgaccaa accaatcgat caccgattgc
     1861 ttgaaaagat tttgcgtgga attgtagatt aaggagaatt ggttcggcat ttcagtggaa
     1921 ggcacatgac gatcccaacc gaacccatcg gtagtattcc caggcctgtt gaacagattg
     1981 cgcgatggcg gtccgggatc cggcactcgc cccgcttttg aaaatgccat ccgcggcagt
     2041 ggcaaagaac gaaaatacgg cggcttgggg attggactct ccctgacgcg aacgatatag
     2101 agttgaacgg cggcacaatc gctgcgcgca gcggcggttt gggtcagggc agccaactca
     2161 ctgtatgttt gccgctcgac atcgcagcag tgagacgcga aaagacgagc cggtcgcggc
     2221 tcaggaggtc cggatggcga aagacaaacg cgtcgccata gtcgaggaca ataaattaca
     2281 ggcgcaatcc ttggcaatac tgcttgaaat gatgggatac caggtgcgca cggcctgcga
     2341 cgcttccact gctatgagta tgatggctga gtttgttccg caggtggcgc ttatcgatat
     2401 cggcttgccg ggactgaatg gttatgaatt ggcgcgacgg ttgcgcgact tgccgcaatt
     2461 gcgcgacatc acgttaatcg cccaaaccgg ttggggaacc gaagacgacc gcgaacaagc
     2521 gcgccaagcc ggtttccagc accatctgac taaaccaatc gaccacaaac ggctcgagga
     2581 aattttatct caagccacgc agtaacggca cggcagggcc gaccgtcttt gcatgccgct
     2641 gcgagacgac cgttgtcgcg gctggtgagt aaatactatt ttttcttgcg cgcctttttt
     2701 tt
//
LOCUS       DATLCH010000142         7462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9347788, whole genome shotgun sequence.
ACCESSION   DATLCH010000142 DATLCH010000000
VERSION     DATLCH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9347788"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VMZ02_04130"
     CDS             <1..711
                     /locus_tag="VMZ02_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HUR71150.1"
                     /translation="IVIIGNEVLSGKTQDINSHFLCTELRQLGVEVQKISTIQDEIEL
                     IGHEVAAFSKRFDYVFTTGGVGPTHDDVTIEGIAHGFHVSVVRHPDIEARMRRRLGAD
                     LNEARLRMANVPEGATLLCADAPFAPIVQIRNVYVFPGIPKILQERFHNIKEQFRDAP
                     YYLKNVYVRHGEGIIATMLNDLLVKYPELMLGSYPVLDLPDYKVKVTLESKDQNYLAR
                     ALEAFVASLPKNSVQRID"
     gene            721..2160
                     /locus_tag="VMZ02_04135"
     CDS             721..2160
                     /locus_tag="VMZ02_04135"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HUR71151.1"
                     /translation="MAETSSWGRLPPFKKAPLPPMPGMLRALGPGVVFMALAQGSGEL
                     IFWPYTIAKYGLTFLFLLIPACLLQFPVIYEIGRYTLLTGESIFQGFIRLNRQFAFIL
                     WILMTLSFLWFGAFAAAGGTSIAALTDFPGGWSRREQTLFWAYATMALFLIAILLSKV
                     IYKFIELFMWSVAIFTLVGLIWASANAEVLGAAPSFVKGLIIPQWPMPRPWDSADATK
                     LLTAVTFAGLGGFWTLFYSYWLRDKGAGMAHYAGRLTGPITGKPEALPESGALPGNDE
                     GLAHVKHWHRFMFWDIGIGVIGNMITTLLTCLLAYALLFPKGLLPQGYEIAVVQSRFF
                     EVSWGGWGRILFLVVAAAFLSDTWLATVDAVSRTHADCVYGFFPGSHRISYRKWYLIF
                     LLLLTLVTGITVQMNEPGTLILISAVIGFVGTVLYSVALVLLNHIYLPRHLPSAAKPG
                     RLSFYLLLLSCTAYFVLAMLYVLSVIKAI"
     gene            2239..2889
                     /locus_tag="VMZ02_04140"
     CDS             2239..2889
                     /locus_tag="VMZ02_04140"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUR71152.1"
                     /translation="MTEIMNWIMESIRAYGPWSVFAGVIIESVIVPIPSPLIIMGAGF
                     ILIGAELQFFGALIPILLQIVLPGAIASTLGAYIGYAIGYYGGKPMVDRWESFLGFSW
                     RDVEALEKRLQDGHVKTTIFFLRALPIFPLSVISATAGLLRLPLKEFSLWTFYGTIPR
                     CLLLGYLGWGLGETYHGIARGIDKAEGIVSGILVLAIMGIIIWLRSRVRGKILDNG"
     gene            complement(2912..3622)
                     /locus_tag="VMZ02_04145"
     CDS             complement(2912..3622)
                     /locus_tag="VMZ02_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71153.1"
                     /translation="MEASIFAAFGLGLVLGVQHALDPDHLIAVSTIVSEHKNFKWASL
                     IGAFWGLGHTTTLFLVGLVVIGLRITIPNSVAAGLEFLVAIMLVILGLNVLWRTFGPA
                     QVHLHSHTHNPETHTHFHVHEKPQQDHAHNHPFKAMRRPFFVGMVHGLAGSAALMLLV
                     LGTIPSPLAALAYIVIFGFGSIGGMLLLSGIISLPFILTARRFSAMNRSIRMIAGFAS
                     VAFGIFLGWGIGAEQHWF"
     gene            complement(3705..4133)
                     /locus_tag="VMZ02_04150"
     CDS             complement(3705..4133)
                     /locus_tag="VMZ02_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HUR71154.1"
                     /translation="MFRVQDEPIDLNELVRYVTDAEAGAIATFIGTTRNNNEGRSVIA
                     LDYESYPEMAERELARIGAEAAQKWQIRRMAIVHRLGPVQIGEASVIIAVSSAHRDAA
                     FAASRFAIEEIKKTVPIWKKEVFEGGEVWIGTQSGQPLQR"
     gene            complement(4140..4385)
                     /gene="moaD"
                     /locus_tag="VMZ02_04155"
     CDS             complement(4140..4385)
                     /gene="moaD"
                     /locus_tag="VMZ02_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HUR71155.1"
                     /translation="MKVRVKFFAILRERAGTAEVVHELTEGATVADLWRDLQQHFPKL
                     DVPGIRLLYAVNQNYVGSNDTLHDGDEVVFIPPVSGG"
     gene            complement(4382..4888)
                     /locus_tag="VMZ02_04160"
     CDS             complement(4382..4888)
                     /locus_tag="VMZ02_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HUR71156.1"
                     /translation="MSVHEHKEKAKSSVRCFVITVSDTRDQTNDSSGQTIKQFLTTER
                     HHLAGYRIVRDEPVEIEALLNEVLGSSDVEVVIVNGGTGIAPRDGTYEVVSRFLDKRL
                     DGFGEIFRYLSYLDIGSAAIMSRAAAGTARGKVLISLPGSKGAVSLAMEKLIVPEIRH
                     MVSQLQGT"
     gene            5328..6488
                     /gene="hemW"
                     /locus_tag="VMZ02_04165"
     CDS             5328..6488
                     /gene="hemW"
                     /locus_tag="VMZ02_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HUR71157.1"
                     /translation="MTREPFSLYIHIPSCISKCPYCDFNSHVVAQIPETGYVDALLRE
                     LEYFGDEIEWRGRTVQSIFFGGGTPSTFKPASIGKLLAWAAATFPIAADCEITMEANP
                     GTVDAAHFAGYRKAGVNRISVGVQSFQTKLLNFLGRVHSADEAKQALNVVKESGFVNF
                     SFDLIYANPGQTLADLEADLDTALEFHPPHLSAYNLTFEEGTPFHREYRAGRIANLDE
                     DEEIAMAELIDSKLSLAGLKRYEISNYSRPWFHSRHNVNYWQAGDYLGLGAGAHSYRR
                     NHDGVTGQRWSNETSPSKYMARVNDTCEAIIDRETIDRLKAAAEFMFLGLRMTDGVRS
                     DLFGQRFGQSPRRFYPQIDGWIDSDLLVESAGFLRFTNKGLMLANSIFVHFM"
     gene            6539..>7462
                     /locus_tag="VMZ02_04170"
     CDS             6539..>7462
                     /locus_tag="VMZ02_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71158.1"
                     /translation="MTTADLNKIRIDHIGSLVRPANLKDVFSRYDRGQADKEELRQAQ
                     DQAIRDVIARQENHGYPIVTDGEFRRHSFQESFSECVAGFDVPKNISLYYEQRDLNQT
                     PLARAEQNFEEAGPAIITRRGAAERLKLVRNLPLEEFRYAQHTAKVPAKITILGPDRI
                     AQRFKWEASLKVYKGLDDFVQHIVEIERQIISELVKSGCKYIQIDAPGYTAYVDKLSL
                     DRMRARGEDPEKNFQRSVDADNALIEGFPGVTFGIHVCRGNARTIDPTTGKLVPQWHR
                     EGSYDAIAEKLFNTLRHHRLLLEYDSDRAGGF"
BASE COUNT         1669 a         2086 c         1945 g         1762 t
ORIGIN      
        1 atcgtcatca taggcaacga ggttctgtcg ggaaaaacac aggatatcaa ctctcatttt
       61 ctctgcaccg aactccgtca gctgggcgtt gaagtgcaga agatttcgac gattcaagat
      121 gaaatagagc tcatcggtca cgaagtcgcg gcgttttcca aacggttcga ctacgtgttc
      181 acgaccggcg gcgtcgggcc gacccacgac gatgtcacta tcgaaggcat tgcgcatggt
      241 ttccatgtca gcgttgtgcg ccatcccgat atcgaagcgc gcatgcgccg tcgcttaggt
      301 gcggatttga atgaggctcg gctgcgcatg gccaatgtgc cggaaggcgc tacactccta
      361 tgcgccgatg cgccattcgc gccgatcgtg cagatacgca atgtctatgt ttttcccggc
      421 atcccaaaga ttctccagga acgctttcac aatatcaaag aacagtttcg cgacgcaccc
      481 tactatctta aaaacgttta cgttcgacac ggcgaaggca ttatcgccac catgttaaat
      541 gacttgttgg tgaaatatcc cgaactcatg ctgggatctt atcccgtact cgacctaccc
      601 gactacaagg tcaaagtgac cttggagtcc aaagaccaaa actatttggc tcgtgcatta
      661 gaagctttcg tcgcatctct ccccaaaaac agcgtccaac gcatcgacta gctagaacga
      721 atggccgaga cttcgtcctg gggcagactg ccgccattca agaaggcgcc gctgccaccg
      781 atgccgggaa tgcttcgcgc actcggcccc ggggtggttt tcatggcgct cgcccaaggc
      841 agtggcgagc tgattttttg gccttataca attgctaaat atggcttaac gtttttgttt
      901 ttgctgatcc ctgcctgcct gctgcaattt ccggtgatct acgagatcgg ccgctacacg
      961 ttgctcaccg gtgaaagcat ttttcagggt tttattcggc tgaatcgtca gttcgctttc
     1021 atcctctgga tattgatgac gctctccttt ctctggttcg gcgcttttgc cgccgccggg
     1081 ggcacctcaa tcgccgcgct gaccgacttc cccggcgggt ggagccgtag agagcaaact
     1141 ttattttggg cgtacgccac catggcgctc tttttaatcg ccatcctgct cagcaaggtc
     1201 atttacaaat tcattgagct tttcatgtgg agcgtggcga tctttacctt agtcggactc
     1261 atctgggcga gcgccaatgc ggaggtgctg ggcgcggcgc ctagtttcgt aaagggtctg
     1321 atcatcccac agtggccgat gccgcgtccg tgggattcag ccgacgcgac aaagttgctg
     1381 acggccgtga cgtttgccgg ccttggtgga ttctggacgc ttttttattc gtattggctg
     1441 cgcgacaaag gcgcggggat ggcccactat gccggacggc tcaccgggcc gattaccggt
     1501 aaaccggaag cgctaccaga gtcgggcgcc ctaccaggca acgatgaagg cctcgcccat
     1561 gtaaagcact ggcatcggtt catgttctgg gacatcggga tcggggttat cggcaatatg
     1621 attaccacgc tactcacctg cttgctcgcc tacgccctgc tattcccaaa ggggctgtta
     1681 ccgcagggat atgagattgc ggtggtgcaa agtcggtttt tcgaagttag ctggggcgga
     1741 tggggccgca ttttgtttct ggtggtggcc gcagcatttc tctccgacac ctggctggcg
     1801 acggtcgacg ctgtcagccg cacccatgcc gattgcgtct acggcttttt ccccggcagt
     1861 catcggatct cctatcgcaa gtggtatcta atatttttac tgctgctcac gttagtcacc
     1921 ggcattaccg tgcagatgaa cgaaccgggc acgctgattt tgatttcggc cgtaatcgga
     1981 ttcgtcggca ccgtccttta ttccgtcgcc ctcgttttgt tgaatcatat ttacctgcca
     2041 cgtcatctgc ccagcgcagc gaaaccagga agattaagtt tctatctctt gcttctttcg
     2101 tgcaccgcct acttcgtgct cgccatgctt tatgttttat cagtgattaa ggccatctaa
     2161 ccgggacccc ctttaagttt aatggcatta aggctgccgc gctggtatta ttggcgaaat
     2221 ttacctggag aattcacctt gacagagatc atgaattgga tcatggagtc tatccgcgct
     2281 tacgggccgt ggagcgtctt tgccggcgtc attatcgagt cggtgatcgt gccgatccct
     2341 tcgccgctga tcattatggg tgcaggtttt attttgatcg gtgccgagct gcaatttttc
     2401 ggagccctca taccgatctt gctgcagatt gtcctgcccg gcgcgattgc atcgacatta
     2461 ggcgcctaca ttggttacgc tatcggctat tacggtggca aaccgatggt cgaccgctgg
     2521 gaaagttttc tcggatttag ttggagagat gtcgaagctt tggaaaaacg ccttcaagac
     2581 ggccacgtta aaacgacgat tttttttctg cgcgccttgc caatatttcc gctgtcggtg
     2641 atctccgcca ctgcgggtct gctgcgattg ccgttaaagg agttcagcct ctggactttc
     2701 tacggtacaa ttccgcgctg cttgctactt ggttatctcg gctgggggct gggcgaaacc
     2761 taccacggca ttgcgcgagg catcgataaa gcggaaggaa ttgtctcggg aattctagtc
     2821 ctggcgatta tggggatcat catttggttg cgttcccgtg tgcgcggcaa gattctcgat
     2881 aacggctgaa cgtggtcgac gcgacaatct gctagaacca gtgttgctcg gcgccgattc
     2941 cccagcctaa aaagattccg aacgccacgc tggcaaatcc tgcaatcatc cgtatcgaac
     3001 gattcatcgc cgaaaaacgt cgagctgtaa gtatgaacgg caagctgata atgccgctca
     3061 agagcaacat cccacctatc gagccaaatc cgaaaatcac gatataagcg agcgctgcga
     3121 gaggcgacgg gatcgtcccg agcacgagca acatcaacgc cgcgctaccg gccaagccgt
     3181 gcaccatacc aacgaaaaac ggtcggcgca tggctttgaa cggatggtta tgagcgtgat
     3241 cctgctgcgg tttttcgtgc acgtgaaaat gggtatgggt ttccggatta tgggtatggg
     3301 aatgcaaatg aacttgcgcc gggccaaagg tgcgccacaa gacgtttagg ccgagtatta
     3361 ccaacataat cgcgacgaga aactcgagac ctgccgcgac tgagttggga attgtgatgc
     3421 gcaatccgat aaccaccaaa ccgacgagaa acagcgtcgt cgtgtggccc aaaccccaaa
     3481 atgcgccgat caacgacgcc cacttgaaat tcttatgctc gctgacgatg gtcgaaaccg
     3541 cgatcaaatg atcgggatca agcgcatgct gaacacccag tacgagtcct aacccaaagg
     3601 cggcgaaaat agaagcttcc atggtgtcaa acgagacaag tgacgagaca gagcctgcgg
     3661 ctgtccacgt cactcaattt tcgatccgca tccgccgcga ttctctatct ctgcaatggc
     3721 tggccgctct gcgtgccgat ccacacttct ccgccctcga aaacctcttt cttccagatc
     3781 ggcacggtct tcttgatctc ttcaatggcg aaccggctgg ccgcgaaagc ggcgtcgcgg
     3841 tgggccgacg aaacagcgat aatgacgctc gcttcgccaa tctgtacggg acctaagcgg
     3901 tgaacgatgg ccatgcgacg gatctgccat ttttgcgctg cttcagcgcc gatgcgcgcc
     3961 agttctctct ccgccatttc aggatacgac tcatagtcga gcgcgatcac gctacgccct
     4021 tcgttgttgt tgcgggtcgt gccaatgaaa gtagcaattg cgccggcttc ggcgtcggtt
     4081 acgtaacgca ccagttcatt aagatcaatc ggttcatctt gcacgcgaaa catattttct
     4141 caacctccgc ttaccggggg aatgaacaca acttcgtcgc catcgtgcag cgtgtcgttc
     4201 gaaccaacat agttctgatt gacagcgtag agcaggcgaa tgcccggaac atcgagcttg
     4261 ggaaaatgtt gctgcaaatc gcgccagaga tcggcaactg tggcaccttc agtgagttcg
     4321 tgaaccactt cggcggtccc ggcgcgctcg cgcaggatcg caaagaactt cacgcgaact
     4381 ttcatgttcc ttggagctgc gaaaccatgt ggcgaatttc cggtacaatc agcttttcca
     4441 tggccaagct gactgcgcct ttcgatcccg gtagcgagat caatactttt ccgcgtgcgg
     4501 ttccagcagc ggcccggctc atgatggccg ccgagccgat gtcgagataa ctcaaatagc
     4561 gaaaaatctc accaaagccg tcgagtcttt tatctagaaa gcggctgacc acttcgtaag
     4621 tgccgtcccg tggcgcgata ccggttcccc cgttgacgat caccacttcg acgtcgctgc
     4681 ttccgagcac ttcgttgagc aatgcctcga tctctaccgg ctcgtctcta acgattcggt
     4741 agcccgccaa gtgatgtctc tctgtagtga gaaattgctt tatggtttgg ccgctgctgt
     4801 cgttggtttg gtcgcgcgta tcgctcacgg taatgacgaa acagcgcacg ctcgatttgg
     4861 ctttttcttt gtgctcatgg accgacatag tcgcctcgca gcggtttttc accggcgact
     4921 ataacaacgg actctggtga attcaaacca gcgaattttc tactcttttc tcgtttgtac
     4981 cctaggccta ccagaggttc tcaaggctca tccgaggtgt tagccgcccg ggtgactagt
     5041 tgattggaga cctcaagtca tcagttaatt caatctatac gctcatggct ttccagattc
     5101 accgcacctt gattagaatg gtgatactac ttgccctgag aaaaggaagc gtattggcat
     5161 ggagaaaaac gtaatcgatt gaacgtatat ccaccgatca ccgcctaatc aacaagcgca
     5221 tcaatcgctg gacatttccg ctaaaatgcg gggaactgcg ctgtcgattc aaagcgagtt
     5281 ccccatgatt gaactccgtc gtgtcgatca ctaggatggg cctgacaatg acgcgcgagc
     5341 ctttttcact ctacattcac ataccctcct gcatcagtaa gtgtccgtac tgtgatttca
     5401 actctcatgt cgtggcacag atcccagaga ctggatatgt cgacgcgctc ctgcgcgagc
     5461 tagagtactt tggtgacgaa attgagtggc gcggccgcac cgtgcaaagt atctttttcg
     5521 gcggcggcac accgtcaact ttcaaacccg ctagcatcgg caaacttttg gcgtgggcgg
     5581 ccgcgacttt tcccatcgcc gctgactgcg agatcactat ggaggcgaat cccggcaccg
     5641 tcgatgccgc acatttcgcg ggttaccgga aagccggggt caatcgcatc agcgtcggcg
     5701 tgcagtcgtt ccagactaaa ctgctgaact tcctgggccg cgtccattcg gccgacgaag
     5761 caaaacaagc tttgaacgtg gtcaaagagt ccggctttgt aaacttcagc ttcgatttga
     5821 tttacgccaa ccccgggcaa actctcgccg atctggaagc ggatctcgac accgctttgg
     5881 aatttcatcc gccgcatcta tcggcttata atctaacgtt cgaagagggg acacctttcc
     5941 atcgcgaata ccgggccggc agaatagcga atctcgacga agacgaagag atcgccatgg
     6001 cggagctgat tgactctaag ctcagcttgg ccggcttgaa acgttacgag atttcaaatt
     6061 attcgcgccc gtggtttcac tcgcgccaca acgtgaacta ttggcaagcc ggcgattatc
     6121 tcggattggg cgctggtgct catagctacc ggcgcaacca cgacggggta accggacaac
     6181 gttggagcaa tgaaacaagc ccgtccaagt acatggcacg ggtcaatgac acgtgcgaag
     6241 cgattatcga tcgagaaacc atcgacagac tcaaagcggc ggccgagttc atgtttctcg
     6301 gcttgcgcat gactgacggc gtccgcagcg atcttttcgg acagcgtttt ggccagtcac
     6361 cccgacgatt ttatccgcag atcgatggct ggatcgacag tgatctgcta gtagagagtg
     6421 cgggattctt gcgcttcacg aacaaagggc tgatgctcgc caattcgatc ttcgtacatt
     6481 ttatgtagga cggaaggccg ccagtgcttc cggcaagtca gtttctctgg agaaaacaat
     6541 gaccaccgct gacctgaaca aaatccgcat cgatcatatc ggcagcctgg tacggccggc
     6601 aaatctcaaa gacgtatttt cccgctacga ccgcggtcaa gccgataaag aagagttgcg
     6661 ccaagcacaa gaccaggcga ttcgcgacgt gatcgcacgg caggaaaacc acggctatcc
     6721 gatcgtaacc gacggcgaat ttcgccgcca tagttttcaa gaaagttttt ccgagtgcgt
     6781 tgccggcttc gacgtgccaa aaaatatcag tctgtattat gaacagcgcg acttgaacca
     6841 gaccccgctc gcgcgcgcgg agcagaattt tgaagaagcc ggaccggcga tcatcacgcg
     6901 acgcggtgcc gccgagcgct tgaaactggt gcgcaaccta ccgctggagg aatttcgcta
     6961 cgcccaacat accgccaaag ttcccgccaa aattaccatc ttggggccag accgcatcgc
     7021 ccaacgattt aaatgggaag cgtcactgaa agtttacaaa gggctcgacg acttcgtcca
     7081 gcacatcgtt gaaatcgaac ggcaaataat ttcagaactc gtaaaatccg gctgcaaata
     7141 catccagatc gacgctcccg gctacacggc ctacgtcgac aaactctcgt tggatcgcat
     7201 gcgcgcgcgc ggcgaggatc ctgagaaaaa tttccaacgt tccgtcgacg ccgataacgc
     7261 tctaatcgaa ggcttccccg gggtaacctt cggtatccat gtctgccgcg gtaacgcccg
     7321 caccattgat ccgaccaccg gtaagctcgt accccaatgg caccgtgaag gttcctacga
     7381 cgctatcgcc gaaaaacttt tcaacacgct ccgtcatcat aggctgctgt tggaatacga
     7441 cagcgaccgc gccggcggct tc
//
LOCUS       DATLCH010000143         4701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8403098, whole genome shotgun sequence.
ACCESSION   DATLCH010000143 DATLCH010000000
VERSION     DATLCH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8403098"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VMZ02_04175"
     CDS             complement(<1..241)
                     /locus_tag="VMZ02_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71159.1"
                     /translation="MGRSTDLRSSLKKRKTSHILAACSIAGLAVVTGLIVPERYHGAL
                     QPVHAASVNAVAQGLPDFINLAKKLGPSVVNVSTTT"
     gene            complement(322..771)
                     /locus_tag="VMZ02_04180"
     CDS             complement(322..771)
                     /locus_tag="VMZ02_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71160.1"
                     /translation="MGRKSAKTAVSKDNRDRQPFWVKDNNGPRMQLDERAMAIFQPDV
                     LIESQYLATQTRRVHLDAERVLMFAVLRDAVSCFQDNVGSDCKRKQAMHRDAEEWILD
                     KDRSHLFSFDNVCEILGLDPEYMRAGLLRWKDTTLRGRRMKARSLAG"
     gene            1021..2199
                     /gene="ribB"
                     /locus_tag="VMZ02_04185"
     CDS             1021..2199
                     /gene="ribB"
                     /locus_tag="VMZ02_04185"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HUR71161.1"
                     /translation="MPLATIDEAIDEIRRGRMVILTDDKDRENEGDLCMAAEKVTPEA
                     VNFMATHGRGLICLTLTEERVKHLGLAMMVSDNTSPFGTAFTVSIDSARGISTGISAA
                     DRAKTILDSIADDAKPQDLATPGHIFPLRARNGGVLVRAGQTEGSVDLARLAGLKPAG
                     VICEIMKDDGTMARQPDLMRFAKKHKLKVVTNADLIQHRLNYDSLVYKAASAPLPTRT
                     GGEFQAIVYNTHVDESEHLALIKGQISANEETLVRVHTKYIPGDVFGFELLNTGAVIQ
                     RSMELIAKAGKGVILYLQPKQKAHHPTMVAYPRVEGKQQKGMNPSFVYQADFKEYGIG
                     AQILRDLGVRKMRLLTNNKKHLVGLRGYGLEVTSLVPIPRDNPLRVAAKSHKPKAKHA
                     "
     gene            2243..2452
                     /locus_tag="VMZ02_04190"
     CDS             2243..2452
                     /locus_tag="VMZ02_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HUR71162.1"
                     /translation="MPIFEYNCASCDHHFEIIVLSPREKISCPKCTGKKVGKQLSVFS
                     APASTSGTAPNPGVCTGTPQSCGCR"
     gene            complement(2591..3118)
                     /locus_tag="VMZ02_04195"
     CDS             complement(2591..3118)
                     /locus_tag="VMZ02_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71163.1"
                     /translation="MADEKARAFEETKWWIDRRAIKERDKAAAPVKGIIEKWAFELLE
                     NYNQDEAAKNLVDINLKIFRELNVEPGQWGYYSLDIGKYPPEKLEKIKAALPLPPSAR
                     GIVIDAGFSVRVMITGEPVGERYVDINFVDTPSLDYWVKAQLIEERVFSNIGEALKRI
                     RADLPSYVIPSEDSR"
     gene            complement(3148..4140)
                     /locus_tag="VMZ02_04200"
     CDS             complement(3148..4140)
                     /locus_tag="VMZ02_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUR71164.1"
                     /translation="MGKIRLTLSCWDYDRTRALQDGRVEVEGVDLTYLPLRVEETFWR
                     MLRYGEFDVAEMSTGSFLMSRDKGAPRLIGIPVFPSKTFRHSCIYINTDSGIKKPADL
                     AGKRVGVPEYQITMATWARGILQHEYGVLPEQMKWFTGGEETPGRQDKVQHKLPSNID
                     IQPIAADKTLSGMLERGEIDAMISAHMPSPFVRRHAKVQRLIPNFREVEADYFKRTKI
                     FPIMHTIVIREEVYEKHPWIAQSLFKAFNESKRICQEAMYEFSALKYMMAWSIDEMEK
                     EREALGPDPWAYGLEANRHILETLVQYTYEQGLISKKLDIDSLFAKSTLEEFKI"
     gene            complement(4171..4371)
                     /locus_tag="VMZ02_04205"
     CDS             complement(4171..4371)
                     /locus_tag="VMZ02_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71165.1"
                     /translation="MEKIDPQPDHLALERFHLGAQVSYKGTLYFIQRRTTLASGEAAV
                     VLQNETDQFVVSASQFLADVRS"
     gene            4501..>4701
                     /locus_tag="VMZ02_04210"
     CDS             4501..>4701
                     /locus_tag="VMZ02_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HUR71166.1"
                     /translation="MGTGAVGGYFGAKLAAGGHDVAFIARGGHLQSLYANGLHVISPK
                     GDLRVKRALFTDDTANIGAVDLI"
BASE COUNT         1111 a         1313 c         1206 g         1071 t
ORIGIN      
        1 ctgtagtggt cgaaacattt actactgacg gtcccagttt tttcgccaaa ttgatgaaat
       61 ccggtaaacc ttgggccacc gcattgacgc tggcggcatg aaccggctgg agagcaccgt
      121 ggtagcgctc cgggacgatc aatccagtta cgaccgccag cccagcgatc gagcaggcag
      181 ctaagatatg cgacgttttt cttttcttca gagatgatct caggtctgtg gaccggccca
      241 tagttttttt cttaccccgc tgtcattagg cgctacgctc gaaacgcaag cgctcgcgat
      301 tgtttgggcg tgtttattgg cttagcccgc cagcgagcga gctttcattc ggcggccgcg
      361 gagcgtcgta tccttccagc gcagtagccc cgcacgcata tactccggat cgagcccaag
      421 aatttcacaa acattatcaa atgaaaataa atgggagcgg tctttgtcga ggatccactc
      481 ttcggcatcg cgatgcatcg cctgcttacg cttgcaatcg gaaccgacgt tatcctggaa
      541 acagctcacc gcgtcacgca gaaccgcgaa cattaagacg cgctcggcgt ccagatgcac
      601 ccggcgtgtt tgcgtcgcta aatattggct ctcgatcagc acatcaggct gaaagatcgc
      661 catcgcccgc tcgtcgagct gcatgcgcgg cccattgttg tctttgaccc agaacggctg
      721 acgatcgcga ttgtctttgg aaactgcagt tttcgcagat tttctcccca tgaaacacct
      781 cccctgagtt gtttagacgg ctcaggggta atcaaggttc caaaaaatcg agaaattctg
      841 gaactacgac cttaccctta agatcaggta atcgcgctgg actactagcc agacagccta
      901 taggcccctg gcaacttgcc tgcaatattc aagaacgccg caggaagcgc gtttcattga
      961 gctctacctc ctcggcgatc tcggttgacg ccactactga agatgataaa tattagcccc
     1021 atgccactag cgaccatcga tgaggccatt gacgagatcc gtcggggccg catggtcatc
     1081 ctcaccgatg acaaagaccg cgagaacgag ggcgatctct gcatggccgc ggaaaaggtc
     1141 accccagagg cggtcaactt catggccacc catggacgcg gtttgatctg cctcacgttg
     1201 acagaagaac gagtgaaaca tttgggattg gccatgatgg tgtctgacaa cacgtcgccg
     1261 ttcggcaccg cctttactgt ttccatcgac tcagcccgag gcatcagcac cggcatctcg
     1321 gcggcggacc gcgccaaaac cattcttgac tcgattgccg atgacgccaa gccccaggat
     1381 ttagccactc cgggtcacat cttccccttg cgggcgcgca acggcggtgt cctcgtacgc
     1441 gccggccaga ccgaaggctc tgtcgatctc gcgcgactgg ccggactcaa gccggccggg
     1501 gtgatttgcg aaatcatgaa agacgacggc accatggcgc gccagccaga cctgatgcgc
     1561 tttgccaaaa aacacaaact taaagtggtc actaacgccg atctgatcca gcaccgcttg
     1621 aactatgact cactggttta caaagccgct tcggcaccgc tccccacccg caccggtggc
     1681 gaatttcagg ccatcgtcta caacacccac gtcgacgaaa gcgaacactt ggcgttaatt
     1741 aaaggtcaaa tttcggccaa tgaagaaacc ctcgtgcggg ttcataccaa atatattccg
     1801 ggggatgttt tcggtttcga actgctcaat accggcgcgg tgattcagcg ctcgatggag
     1861 ttgatcgcca aagccggcaa gggcgtgatc ctgtatttgc agccaaagca aaaggcgcat
     1921 catccgacga tggtcgccta tccgagagtt gaaggaaaac agcaaaaagg aatgaatcca
     1981 tcgttcgttt atcaggcgga tttcaaggag tacggaatcg gcgcgcagat actccgcgat
     2041 ttgggtgtgc gtaaaatgcg cctgctcact aacaataaaa aacatctcgt cggactacgc
     2101 ggctacggct tggaagtaac ctcgctggtg ccgatccccc gcgacaatcc gctgcgtgtt
     2161 gccgccaaga gtcacaaacc caaagccaag cacgcttaac cgtcgtaatc tgaaaattgc
     2221 ctaggtattt aagcagcgcc ctatgcccat attcgaatac aactgcgcca gttgcgacca
     2281 tcattttgag atcatagtgt tgtcgccccg tgaaaaaatc agctgtccca aatgcaccgg
     2341 caagaaagtc ggcaaacaac tgtcagtctt cagcgcgccg gcatcgacca gtggcacagc
     2401 gccaaatccc ggtgtttgta cgggaacgcc gcaaagctgc ggctgccggt aagaaacatc
     2461 gcacaattgc tagccactac ttatttttac attcgggttg gacaagaatt ctagcgcgga
     2521 tccgcctgcg ccgcacttcg gatgttggaa taccgcaaag ctgtggccga atgaacggcg
     2581 cggcaattga tcacctagaa tcttcagacg ggattacata gctcggcaag tcggcacgga
     2641 tgcgcttgag cgcttcgccg atgttcgaaa acacgcgctc ttcaatcaac tgcgctttga
     2701 cccagtaatc gagtgatgga gtatcgacaa aattgatatc gacatagcgt tcgcccaccg
     2761 gctcgccagt aatcattaca cgcaccgaga agccggcatc gatgacgatg ccgcgcgcgc
     2821 ttggtggcaa cggtagcgcc gctttgatct tctccaattt ttccggtgga tatttgccga
     2881 tatccagaga gtaatacccc cactggccag gctcgacatt tagctcccgg aaaatcttta
     2941 ggttgatatc gacgagattt ttcgcggctt cgtcctgatt gtaattttcc aggagctcaa
     3001 aagcccattt ttcgatgatg cctttaaccg gcgccgctgc tttgtcgcgc tccttgatcg
     3061 cgcggcgatc aatccaccat ttagtttctt cgaatgcgcg agctttttcg tcagccataa
     3121 ttttgctctg ccgtcactgt gcggagccta gatcttgaat tcctctagcg tgcttttggc
     3181 gaacagcgaa tcgatgtcga gcttcttgct gatcaaacct tgctcgtatg tgtattgcac
     3241 caaggtctcg agaatatggc ggttggcttc cagaccataa gcccaggggt cgggacccag
     3301 ggcttcccgt tctttttcca tctcgtcaat ggaccacgcc atcatatact tgagcgccga
     3361 gaactcatac atcgcctcct gacatatcct tttagattca ttgaaagcct taaaaaggct
     3421 ctgggcgatc caagggtgtt tctcataaac ttcttcgcgg attacgattg tgtgcatgat
     3481 cggaaaaatc ttggtgcgct tgaaataatc cgcttccacc tcgcggaaat taggtatgag
     3541 ccgctggact ttcgcatgac ggcgtacaaa cggcgacggc atatgcgccg aaatcatcgc
     3601 gtcgatctcg ccgcgttcga gcataccgga aagcgtcttg tcagccgcaa tcggctggat
     3661 gtcgatgttg gacggcagct tgtgttgcac tttgtcctgg cgccccgggg tctcttcgcc
     3721 gccggtaaac catttcattt gttcaggcaa gacaccgtat tcatgttgca agatgccgcg
     3781 cgcccaggtc gccatggtaa tctggtattc gggaacgccg acgcgcttgc ccgccaggtc
     3841 cgccggtttt ttgatgccgg aatcggtgtt gatgtaaatg cacgaatgac gaaacgtctt
     3901 ggacggaaaa actggaatgc caattaatct gggagcgccc ttgtcgcgtg acatgaggaa
     3961 tgaaccggtg gacatctcgg cgacatcgaa ttcgccgtag cgtaacatcc gccaaaaggt
     4021 ttcttccacg cgcagcggca agtaggtcag atcgaccccc tcgacctcaa cccgcccatc
     4081 ctgcaaagca cgcgtgcgat catagtccca gcaagataat gtcaaacgaa ttttgcccat
     4141 gatgaactcc tgttgaataa ttactcgaag ctaggaacga acatcagcaa gaaattggct
     4201 ggcgctgacg acgaattgat cggtttcgtt ttgcaagacg accgcggctt cacctgaagc
     4261 cagcgtggtg cggcgctgga taaaataaag cgtgcccttg taactcactt gggcgcccag
     4321 atggaaacgc tcaagagcta aatgatccgg ctggggatcg atcttttcca tgaccaactt
     4381 ctataacata gcacccaaca agcttaaact accgaagcct tccacgagtg gaaaattgac
     4441 aagcgccaag gcgcaagtct acagttaccg gaagagataa ttctaatgcg tatcgcggtc
     4501 atgggtacag gagctgtggg gggctatttc ggcgcgaagc tcgcggctgg agggcatgat
     4561 gtcgctttca tcgcccgcgg tggacacctg caaagtctct atgcaaacgg tctccacgtc
     4621 attagcccca agggcgattt gcgagtgaag cgtgccctct tcactgacga tactgccaat
     4681 attggcgcgg tggatctgat t
//
LOCUS       DATLCH010000144         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1158882, whole genome shotgun sequence.
ACCESSION   DATLCH010000144 DATLCH010000000
VERSION     DATLCH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158882"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VMZ02_04215"
     CDS             complement(<1..452)
                     /locus_tag="VMZ02_04215"
                     /inference="COORDINATES: protein motif:HMM:NF025044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HUR71167.1"
                     /translation="MTMYPAPLARLIQELSKLPGIGEKSAARLAFHMLKSKRDDVFRL
                     SDSIGRLRQEMGLCDRCFGFSEVHQPQNENGVQCDVCRNSEREGDKVCVVEDPADMIA
                     IEKSQEYRGQYHVLHGTISPLDGIGPDALRIKELLDRLRDGTIKEVIVA"
     gene            complement(541..864)
                     /locus_tag="VMZ02_04220"
     CDS             complement(541..864)
                     /locus_tag="VMZ02_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HUR71168.1"
                     /translation="MASIPGMGNLLKQAQDMQARMAKVQEELAHKTVEGSSGGGMVQV
                     TVNGQFNLTAVKIEPSVISVDEKGMLEDLLLAAVNDGMRKAREMASAEMAKLTGGLKI
                     PGLMP"
     gene            complement(917..2599)
                     /gene="dnaX"
                     /locus_tag="VMZ02_04225"
     CDS             complement(917..2599)
                     /gene="dnaX"
                     /locus_tag="VMZ02_04225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HUR71169.1"
                     /translation="MSYLVLARKWRPQNFEDVAGQEPITRTLKNAIKANRIAHAYLFT
                     GVRGVGKTTAARILAKALNCERGPTATPCNQCTHCQEITGGNSIDVLEIDGASNRGID
                     EIRQIIENVRYQPAKCRFKIYIIDEVHQVTKDAFNALLKTLEEPPPAVKFILATTEPQ
                     RLPETILSRCQRYDFRRISLREIVERLSAIGKSEGLNITDEALVLLGREADGSMRDAQ
                     SLLEQVLACAMPAAGENQQATVNAAMLQEILGLAERKCLYDLSQAVLDGDPRRCVELV
                     AEVMNQGRDLSRLSRDLVEHFRNLLVARLSASRSSGNRGTDVSQLLDLPDQEIADLVR
                     QTDKISAEALLDYLDFMAAGDEEVARSATPRFALEAVLIRLATLPQSLPVAEILTRLE
                     RLEGHSRSGNLRPAASTERSKTASIPASVMSTSVAPSPNDVVSSANDDTNKWHEFVAF
                     VGKEKKFLASHLSATSAVSLPPAPLKVIVAERHHLGFLQDADNFLSLRTLAKQFFGQD
                     VTIQFVASTGDSAPPITADASATGGAAGDRSEMVKEALRIFGGSVRSVRRET"
     gene            complement(2709..2806)
                     /gene="ffs"
                     /locus_tag="VMZ02_04230"
     ncRNA           complement(2709..2806)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VMZ02_04230"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2948..3038)
                     /locus_tag="VMZ02_04235"
     tRNA            complement(2948..3038)
                     /locus_tag="VMZ02_04235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3002..3004),aa:Ser,seq:gga)
BASE COUNT          686 a          899 c          842 g          727 t
ORIGIN      
        1 gcgacgataa cttccttgat ggtgccatcg cgcagccgat cgagcagttc tttgatgcgc
       61 aatgcgtcag gaccgatgcc gtcgaggggc gagatcgtac cgtgcagcac atgatactgg
      121 ccgcgatact cctgggactt ctcgattgcg atcatgtcgg cgggatcctc gaccacgcag
      181 accttgtcgc cttcgcgctc tgagttgcgg cagacgtcgc actgaacgcc gttttcgttc
      241 tgaggctggt gcacttcgct aaaaccgaaa cagcggtcgc acaagcccat ttcctggcgc
      301 agccggccaa tgctgtcgga gagacgaaag acatcgtcgc gtttgctctt caacatgtga
      361 aacgccaatc gtgctgccga tttttcgccg atacccggga gcttggacag ctcctgaatc
      421 aaacgcgcca agggcgcggg atacattgtc atgagcaggc ctcgcacggt gaggctatga
      481 tactcttcgt cttcatattt cgagctggct aatggcgcta gtgaatttgt cgcgaggtaa
      541 ttacggcatc aacccgggaa tctttaagcc gccagtgagt tttgccatct cggctgatgc
      601 catctcacga gctttgcgca tgccgtcgtt aaccgcggca aggagcaaat cttcgagcat
      661 gcctttctcg tcgacgctga taaccgacgg ctcgattttc accgcggtca gattgaactg
      721 gccattcacc gtcacctgga ccataccgcc tcccgaggag ccttcgaccg tcttgtgcgc
      781 cagctcttct tgaaccttgg ccatgcgcgc ctgcatatcc tgcgcttgct taagcagatt
      841 gcccattccc ggaatgctcg ccatattccc tcctagtgcc tcctacttgg acgcggcaat
      901 gacccggttc agcagttcaa gtttcccgcc gcaccgaacg gaccgagccg ccgaagatac
      961 gcaacgcttc tttgaccatt tcgctgcggt cgcccgcggc accacctgtc gccgacgcat
     1021 cggctgtgat gggaggtgcg ctatcgccgg tcgaagcgac gaattggatc gtgacatctt
     1081 gaccgaaaaa ctgcttggcc aacgtccgca gcgaaaggaa attgtcggca tcttgaagaa
     1141 atcccagatg atgccgttcg gcaacgatga ccttgagcgg cgccggcggc aacgagactg
     1201 ctgaagttgc acttaaatga gatgccaaaa actttttttc cttgccgacg aaagcgacga
     1261 attcatgcca cttgttagtg tcgtcgtttg ccgaagagac aacatcgttg ggtgatggcg
     1321 ctacggaggt agacatgacc gacgccggaa ttgaagcggt cttggaacgt tccgtcgacg
     1381 ctgcaggcct aaggttcccg ctacgagaat gcccttccaa tcgttccagc cgcgtcagta
     1441 tttcggcgac gggtagggac tggggcaagg tggccagccg gatcaatacc gcttcgaggg
     1501 cgaaacgggg ggttgccgag cgcgcgacct cttcatcgcc cgcagccata aaatcgagat
     1561 aatcgagcag cgcttccgcc gatatcttat ccgtctgtct gactaagtcg gcaatttcct
     1621 ggtctggcag atcgagcaat tgagaaacat ccgtccctcg attaccgcta gatcgactgg
     1681 ctgataaacg cgccactagc agatttctaa aatgttcgac taagtcgcgc gacaaacggc
     1741 taagatctcg gccctgattc atcacctcag cgaccaactc gacacaccgg cgcggatcac
     1801 cgtcgagcac cgcttgagac agatcataaa gacacttacg ctcggcgagg ccaaggatct
     1861 cttgcagcat ggccgcgttg acggtcgctt gctgattttc acccgccgca ggcatagcgc
     1921 aggctagcac ctgctcaagt aatgactgag catcgcgcat gctgccgtcg gcttctcgcc
     1981 cgagtaggac cagagcctca tctgtaatat ttagcccttc gctcttgccg atggctgata
     2041 gtcgctcgac gatttcgcgc aaactgatgc gccgaaagtc ataacgctgg cagcgcgata
     2101 aaatggtttc gggcaaacgc tgtggctccg tcgttgcgag gataaattta accgctggtg
     2161 gcggctcctc cagcgttttc agcagagcgt tgaaagcatc tttggtgacc tgatgcactt
     2221 cgtcgataat atagatcttg aagcggcatt tagccggctg atagcggacg ttttcaataa
     2281 tttggcggat ctcatcgatg ccgcggttcg aggcgccatc gatctcaagc acatcgatcg
     2341 agttgccacc tgtgatctct tggcagtgag tgcattgatt acacggcgtc gccgtcggtc
     2401 cgcgttcgca gttcagcgct ttggcaagta tccgcgcagc ggtcgtctta ccgacgccgc
     2461 gcaccccggt aaagagatag gcgtgggcaa tgcgattggc tttgatggcg tttttgagtg
     2521 ttcgggtgat cggttcttgg ccggcgacat cctcaaaatt ttgtggccgc catttccgcg
     2581 ccaagaccag gtaagacata aatcccttgg aagttttaaa ctaaaagctg ctggcgctga
     2641 attcaccagc cattgatcca cgatccgcga ttaatactga gaaccacgag gtgaggatgc
     2701 ccattgctga cagccaggtg cccctgtggc acaggggaga gccgttaccg ctgcttcctt
     2761 ccggacctga cggagttcac ggggccgcct tgcacaggac ctaactatca gcaatcgaca
     2821 tccgcgttat aaacactgct gaagattcag ctataaaatc ctacgtggaa aacttgagag
     2881 tttcaagttt tcttgtacgt ccgtcatgaa ttccaaaaga accgccgccg gccgactcct
     2941 gggataatgg cggagaggga gggattcgaa ccctcggtac ccttttgggg tacacacgat
     3001 ttccagtcgt gcgccttcaa ccgctcggcc acctctcctt atttacaatt caaagacttg
     3061 cgcgccctgc cctgctctct taatcagcga tgtctccaaa atgtctgcaa acaactattc
     3121 tcagggaatc ttttaagtat tgcgaaagtt atca
//
LOCUS       DATLCH010000145         8309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4131506, whole genome shotgun sequence.
ACCESSION   DATLCH010000145 DATLCH010000000
VERSION     DATLCH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4131506"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VMZ02_04240"
     CDS             <1..898
                     /locus_tag="VMZ02_04240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71170.1"
                     /translation="VGFSHVWVGDSHLIWREAYINMAAMALNTTKVKLGTGVTNPLTR
                     HPSVVASGYATLEEYGPGRMIVGIGLGDSSVETMGMKPSTLANFEKSLQQMRELFAGK
                     EAELPTGKIHLLHPCKNKVPIYVAASGPKMLELCGRIADGIIVLVGVADDYISHAKEK
                     IAAGAKAAGRKLEEINLVLWVPCAVSDTANAKDAVKAHVARVVAHPLPYVLDPNEQKI
                     LQEIRKTYDYYHHMDQQANHAEVIPDWLVDKFAIAGTVAECKAQIGRIKKTGIQQIAI
                     IPYSPAGGSREETIRGFARAMQ"
     gene            944..1336
                     /gene="gloA"
                     /locus_tag="VMZ02_04245"
     CDS             944..1336
                     /gene="gloA"
                     /locus_tag="VMZ02_04245"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HUR71171.1"
                     /translation="MKIVHTMIRVNNLDESLKFYCDGLGMKLLKKRDYESGRFTNVFV
                     GYGSEDTDAVIELTYNWDTHAYDIGNGFGHIALGVPDVYKTCEDLRAKGVKITREPGP
                     MKHGSGANAIAFLEDPNGYKIELTTRAD"
     gene            complement(1523..2008)
                     /locus_tag="VMZ02_04250"
     CDS             complement(1523..2008)
                     /locus_tag="VMZ02_04250"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HUR71172.1"
                     /translation="MDGTIIYWVGAVLLVGLGLVGLVLPGLPGAPLILIGLIAAAWGE
                     NFTYVGLWTLLIITLLAGLTYAVDFWAAIFGAKKFGASKRAIIGALIGVVVGLFLGFA
                     GVIFGPFIGAVMGELSARRDLQQGIRAGIGATIGLVIGSALKIALAFAMIGIFAVVRF
                     L"
     gene            complement(2055..2816)
                     /locus_tag="VMZ02_04255"
     CDS             complement(2055..2816)
                     /locus_tag="VMZ02_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71173.1"
                     /translation="MNETLRAPSPAYVMREARGLMELPRLLLRFPSLARQPRGNGEPV
                     LILPGYGAGDGSTMILKGYLRLLSYRARGWGLGRNSGTVVDSLPRLLRRLGNLSRRSK
                     QKVTIIGWSFGGYLARELARERPDLVRLVITLGTPVIGGPKYTVAAASYRKRGIDIDV
                     IAAEVEQRNRMANLEVPVVAIYSRRDAMVAWQACIDSLTPNIEHIEVQATHIGFGFSA
                     DVYKLIAQRLTHHAGCDGAPTTHQRHRLRLEKLPR"
     gene            3159..3917
                     /locus_tag="VMZ02_04260"
     CDS             3159..3917
                     /locus_tag="VMZ02_04260"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR71174.1"
                     /translation="MAAERRPAVTDAEWEARVNLAAAYRLVALYGWDDLVFTHISIRV
                     PDAQHHFLINPYGMLFEEITASSLVKVDIDGNIVMDTPYPISPAGFVIHSAIHAARES
                     AKCVLHTHSVNGIAVSAQQTGVLPISQQSAYVLSSLGYHDYEGFAVNDEEKPRLVRDL
                     GDSIFLILRNHGLLTVGETVADAFVAMYVLETVCTIQVRAQAGGGELIAIDPRIIEGA
                     KEQARKATTGVSAGALSWPGLLRKLDRTDPSYKN"
     gene            complement(4155..5441)
                     /locus_tag="VMZ02_04265"
     CDS             complement(4155..5441)
                     /locus_tag="VMZ02_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HUR71175.1"
                     /translation="MSTRKPAGAVDYLNNRESRRHIMGIDMSHRYSGVTTRLLDRDER
                     LIDTEEPTPLVIERRGSSSSLGFLREFLSSNSSQIVMDIATHGAVLLRGFDLRSPQDF
                     ESAVLAITNFQGINKCLMSEPGRDVVKGTRFVLHTNSLYRTGGSLYLGGFHSENYYSP
                     DVPSYISFFCEQASQLGGETGLVNTAKLFSDLPEALKTKLTERAYWIIAWPLAKMAAR
                     YGVNAQQMEQFCLDSGLPIVTRQGQKYASMYKPCVIEHPLTKERALAFNYSVELLKSG
                     LDRAIQRLFLPSYSSKEWLLHRLSWRFPVIGVLDKLDVLSRDPGTVWRSLFRTPFDVC
                     VSEIFDKEAVGRLAMSIRNRYSSFKWRASDVLLVDNLKIAHGGMPGSGARTLRVLITN
                     PIRMRYDSKGPGVHVVSHDSYHPTLAAQLSARHKIS"
     gene            complement(5572..5922)
                     /locus_tag="VMZ02_04270"
     CDS             complement(5572..5922)
                     /locus_tag="VMZ02_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71176.1"
                     /translation="MWLDIAVTLLLGGLGWFGIKYYSLAGKLLLSFAAIWQGFASFLT
                     IMCGIFRPRRLRNHHRQAYMKNLVRSLAKEYAGTSMTKALAAGRSATLDEELFYRRFI
                     RQKWNLIAGRKQHS"
     gene            complement(5974..6651)
                     /gene="nth"
                     /locus_tag="VMZ02_04275"
     CDS             complement(5974..6651)
                     /gene="nth"
                     /locus_tag="VMZ02_04275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HUR71177.1"
                     /translation="MTQKKPNSPGLATSDRLKKIVARLEKTHPDAKLDLDFTSPLELL
                     IALILAAQARDDLVTKITPELFKTNRTAADYAKQPLPELEKQIGKINFYRNKAKSIHN
                     CCTELVERFGGKVPDNLEDLVSLPGVGRKTANVVLGNGFGRQTIGVDTHVMRLSQRLG
                     FTSQTDPDKIETDLMAIVPPKQRVRFCHLMQYHGRRICVAKKPKCPECSIEDLCPYPG
                     KTKSSKP"
     gene            6711..7028
                     /locus_tag="VMZ02_04280"
     CDS             6711..7028
                     /locus_tag="VMZ02_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71178.1"
                     /translation="MDATALTTAKQNIRQLKKQKADLAAATPDRKKTRVLQRKIRLLK
                     RTTRVLAGEKKALAAKAAADAASKEAAEKAAAAKAAADAKAAEAKAAADAAKAGEAAD
                     ASA"
     gene            7040..8095
                     /locus_tag="VMZ02_04285"
     CDS             7040..8095
                     /locus_tag="VMZ02_04285"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HUR71179.1"
                     /translation="MNLPPPLRGRGSATNPKNRFESIERVPEPPEDSDEVSAPHTQFF
                     PDSSKSLIAFNNSPDVGFDASINPYRGCEHGCVYCYARPTHEYLGFSAGLDFETKIMV
                     KEDAPELLRKELARPKWQPQLVALSGNTDCYQPVEKHKQLTRRCLEVFLEFRNPVVII
                     TKNHLVTRDIDILAQLAKFDCIAVTISVSTLDAKLAASMEPRASRPSRRLDAIRQLAD
                     AGVPVGYLQAPMIPGLTDAEAPAIAIAAARAGATFSGYVALRLPFAVKELFEQWLTQH
                     YPDKKDKVLNRVREIRGGKLNDPNFESRMRGEGKFAEQMAALFQLARKKSGITDRWPK
                     LTTEHFRRPEKDQLRLF"
BASE COUNT         1958 a         2233 c         2314 g         1804 t
ORIGIN      
        1 cgtcggcttc agtcacgtct gggtcggcga ttcacattta atttggcgcg aagcatacat
       61 caacatggcc gcgatggcgc tcaacacgac aaaagtgaag ttgggcaccg gtgtgaccaa
      121 tccgttaacg cgccatccgt ccgtggtcgc gagcggctac gcgaccttgg aagaatatgg
      181 accgggccgg atgatcgtcg gcatcggctt gggcgatagc tcggtggaaa ccatgggcat
      241 gaagccgtcg acgctggcca attttgaaaa atcactacaa cagatgcgcg aactgttcgc
      301 cggaaaagaa gccgaactgc cgaccggcaa aatccatctc cttcatccat gcaaaaacaa
      361 agtgccgata tacgtcgcgg ccagcgggcc gaaaatgctg gaactctgcg ggcgtattgc
      421 cgatgggatt atcgtcctgg tcggtgttgc cgatgattac atctcgcatg ccaaagagaa
      481 aattgccgcg ggcgccaaag cggcgggccg aaagttggaa gagattaatc tagtcctatg
      541 ggtgccctgc gcagtttccg ataccgcgaa tgcaaaggat gcggtcaaag cccatgtcgc
      601 tcgcgtagtc gctcatccgc tgccttatgt cctcgatccg aacgaacaga aaattctgca
      661 agagatccgg aagacctacg attactacca tcacatggat cagcaggcta accatgccga
      721 agtgattccc gattggctgg tcgacaagtt cgcgattgcc ggtacggtag ccgaatgcaa
      781 agcgcagatc ggacgcatca aaaaaaccgg catccagcaa atcgcgatca ttccttacag
      841 ccccgcgggg gggagccgcg aggaaaccat cagaggtttt gcgagggcga tgcagtgatt
      901 aagttcgcga ggtattttaa gtagcccaac cgacggaggg attatgaaga tcgtccacac
      961 aatgattcgc gtcaacaatc tcgacgagag tttgaaattc tactgcgatg ggctcgggat
     1021 gaagcttttg aaaaaacgtg actatgagtc gggccgcttt accaacgttt tcgtcggcta
     1081 cggttcggaa gataccgacg cggtaatcga gcttacctac aactgggata cccatgctta
     1141 cgatattggc aatggcttcg gccatatcgc gctcggtgtg ccggatgttt acaaaacctg
     1201 cgaagatctg cgtgccaaag gcgtgaaaat cacccgcgag cccggtccga tgaaacatgg
     1261 cagcggcgcc aacgccatcg catttctcga agatcctaac ggatacaaaa tcgagttaac
     1321 gacacgcgcg gattaactga cgaaaccaag atttgttcca acgatcattg attcgcaatg
     1381 gccactttcg tcgcaaaccc gaccgtcatt cctgcagcag gccagccgcc aaaaatcatc
     1441 gaggagttca ttggtcgcgt caattcacag acctcagggg gaacagctac acgcgccgaa
     1501 atcgctgtga aggcaagcgg tgtcataaga atcgcacgac cgcaaaaatt ccgatcatgg
     1561 caaacgccag cgcgatcttc agcgcagagc cgatgactag gcctatagtg gcgccgatgc
     1621 cggcgcggat gccttgttgc aggtcgcgac gggctgagag ttcgcccatc accgcgccga
     1681 taaagggacc gaagatgacc ccggcgaagc cgagaaacag accgacgacc acgccaatga
     1741 gggcgccgat gatggcgcgt ttggatgcgc cgaacttctt agcgccaaag attgccgccc
     1801 aaaaatcgac cgcataagtc aatcctgcta aaagcgtaat gattagcagc gtccacaggc
     1861 cgacgtaagt aaaattttca ccccaggcgg cggcgatgag gccgatcaaa atcagcggtg
     1921 cgccgggcag tccggggagg acaagtccga ccaaacctag acccaccaga agaactgcgc
     1981 caacccagta gataatggtg ccatccatgg gtgtgctctc ccgtcgttgt tgagcgtagc
     2041 tgcggtgatt ggcttcagcg cggcagcttc tctagtctca agcgatggcg ttgatgcgtc
     2101 gttggtgcgc catcacatcc cgcgtggtgc gtgagccgct gcgcgatgag tttataaacg
     2161 tcggcggaaa aaccgaaacc gatgtgagta gcttggactt cgatatgctc gatgttgggc
     2221 gtcagactgt cgatgcacgc ttgccaagcc accattgcgt cccggcgaga atagatcgcc
     2281 acaaccggaa cttccagatt cgccatccga ttgcgctgct ccacctccgc ggcgatgacg
     2341 tcaatatcga tccctcgctt acggtaggat gccgccgcga ccgtatattt ggggccgccg
     2401 atcactggcg tgcctagggt aatcaccagg cgaaccaggt cgggccgctc gcgagccagc
     2461 tcgcgggcta agtatccgcc gaagctccag ccgatgatgg tgactttttg tttgctgcgc
     2521 cgcgacaaat tgcccaatcg tcgcaagagc cgcggcaaag aatcgacgac cgtgccgcta
     2581 tttcgcccca gaccccagcc gcgcgctcga tagctaagta gacgtaaata tcccttgaga
     2641 atcatcgtcg agccatcgcc ggcgccatag cctggaagga tcagaaccgg ttcgccgtta
     2701 ccgcgtggtt ggcgagccag ggatggaaat cttaacaata agcgcggtag ctccatcaag
     2761 ccgcgcgctt cgcgcatcac ataggccggt gagggtgccc gtagagtttc attcatgatg
     2821 cgcagccaga catcgattcc caatgctagc caaatttgtt catgggaaca atggtaaaat
     2881 ggtcaaccgg agaatttccg ccatcaaaac cggtgttggg acatgaggct agtatcggcc
     2941 cggaccgagg ttacggcatt tgattcatcc caccggtcat agttttccgc cgtgacccgg
     3001 gttcgcgcat gcaacaattt gggcttgacg atggacgttc acgagctttg tcgaccttga
     3061 caagttattc attgtacggc taaaggtcgg gcaaacgcgg tgtttggaag ccttgatgtt
     3121 gaagtcattg cccggtcgag cgtcaaaagg aaatctccat ggctgcagag cgtagaccgg
     3181 cggtaactga cgccgaatgg gaagcacggg ttaacctcgc tgccgcgtat cgcctggtgg
     3241 ccctgtacgg ttgggacgat ctcgttttta cccacatttc gattcgcgtt ccggatgctc
     3301 agcatcattt cctcatcaat ccttacggga tgctgtttga agagattaca gcgtcgagcc
     3361 tggtcaaggt cgacatcgac ggcaacatcg tgatggacac gccttatccg atctctcccg
     3421 ctggttttgt tattcacagt gctatccatg cagcgcgcga gagtgcgaag tgcgtgctac
     3481 atacccacag cgtaaacggg atagcggtgt cggcacaaca gaccggggtt ttaccaatat
     3541 cgcagcaatc tgcctacgtg ctctcctcgc tcggttatca cgattacgag ggatttgctg
     3601 tgaacgacga agaaaagccg cgattggttc gtgatctcgg ggacagcata tttttgatac
     3661 ttcgtaacca tggcttgttg accgtcggcg aaacggttgc ggatgccttt gtggctatgt
     3721 acgtgcttga gacggtgtgt acgattcaag tgcgcgctca agccggcggc ggcgaactca
     3781 tcgccatcga cccaaggatt atcgaagggg ccaaggaaca ggcgcgaaag gcgaccactg
     3841 gtgtaagcgc gggcgcgctc agttggcctg ggctgcttcg taagctggat cgaactgatc
     3901 catcgtacaa aaactaagtt ctctaaggaa cggcacgagc atatcccaag caggaaagct
     3961 catagcccga aggcttttgc cgccagtggt cctgcccact attcgaatgg ccaagcaacc
     4021 ggctcgcagt caataatttg cgctgagcga gaggcagggc aagcaaatag ctcgatggcc
     4081 gcaacgagaa aacttgtcgg cacgctgtaa caccgaaaga gtagcactcc ccgcaacttt
     4141 tcccctgacc tctcctaaga gattttgtgg cgagcgctaa gctgagctgc aagcgtagga
     4201 tgataagaat cgtggctgac gacgtgaacg ccgggaccct tcgaatcgta gcgcatccga
     4261 atcggattcg taataagaac tctcagcgtc cgcgcccccg acccaggcat gccgccgtgc
     4321 gctattttca gattgtcgac gaggagcacg tcgctggctc gccacttaaa cgaggaatac
     4381 cgattgcgga tgctcatggc caaacgaccc acagcctctt tatcgaagat ctcgctaacg
     4441 cacacatcaa aaggcgtgcg aaataagctt cgccatacgg ttcctggatc ccgagacaac
     4501 acgtcgagtt tatccaacac gccgataacg gggaagcgcc agctgagtcg atgcagcagc
     4561 cattccttcg acgagtaact cggcaggaat aatctttgaa tcgcgcggtc gagtccggac
     4621 ttcagcaatt ccactgagta gttgaatgcc aaggcgcgct ccttagtgag cggatgttcg
     4681 atcacacacg gcttgtacat cgaggcgtat ttctgacctt gtcgcgtgac aataggtaac
     4741 ccggaatcca aacaaaattg ctccatctgc tgagcattaa cgccatatcg cgccgccatt
     4801 ttcgctaacg gccacgcgat gatccagtag gctcgttccg tgagttttgt tttaagggcc
     4861 tcgggaaggt cggagaaaag cttggccgtg tttaccagac ccgtttcgcc gccaagctgc
     4921 gatgcttgct cgcagaaaaa cgatatatag cttggtacgt caggcgaata gtagttttcc
     4981 gaatggaaac cacccagata taggctccct ccagtccgat ataaactgtt ggtgtggagg
     5041 acgaaacgag tgccttttac gacgtcacga cctggctccg acattaggca tttgttgatt
     5101 ccttgaaaat tagtgatagc aaggacggca ctctcgaaat cctgggggct cctcaagtcg
     5161 aagccgcgca ataggacggc tccatgagta gcaatatcca taactatttg agatgagttc
     5221 gacgagagaa attcccgaag aaaaccgagg ctactcgaac taccgcggcg ctctatgacg
     5281 agcggtgtag gctcttcagt atcgatgagc ctctcgtcgc gatccaggag ccgagttgtg
     5341 acgccgctgt agcgatgact catgtcgatg cccataatgt gtcgtctgct ttccctgttg
     5401 tttaggtagt ccacggcccc tgcgggcttc ctggtactca tgagagggca gctgactttt
     5461 gagcaggctt ttcattggtc agtaagtgcc agaattcgcg ttaacgcgga acttaatcga
     5521 ttgtccaaag ccatgtcaac atttcgtggc ccggacaccg acaaaaaatt atcagctatg
     5581 ctgttttctc ccggcgatca ggttccattt ctgccgaata aagcggcgat aaaataattc
     5641 ttcatctagc gttgcgctac gacccgctgc tagcgctttt gtcatggaag ttccggcata
     5701 ctctttagct aatgatcgaa cgaggttttt catataggct tgcctgtgat gattgcgtag
     5761 cctccttgga cgaaaaattc cacacatgat tgtcaggaaa cttgcgaatc cttgccatat
     5821 ggcagcgaac gataacagta actttccagc cagagagtag tatttgattc cgaaccagcc
     5881 gagcccgccg agaagtaatg tcacggcaat atcgagccac aactatttct ctacgtagaa
     5941 aagcacggcg gggtattgaa ttctctgtgt ggcttagggc ttggagcttt tggtctttcc
     6001 gggatacggg cagagatctt cgatcgagca ctctgggcat ttcggttttt ttgcaacgca
     6061 gatgcgccgg ccgtgatact gcatgagatg gcagaaacgg acgcgctgtt tcggcgggac
     6121 gattgccatc agatcggttt cgattttgtc cggatcagtc tggctggtga agcccaaacg
     6181 ttgcgacagg cgcataacat gggtgtccac cccgatggtt tgccttccga agccgttacc
     6241 gagaacgacg ttcgcggttt tacggccgac acccggcaga ctgacaaggt cttctaagtt
     6301 gtcgggcacc ttgccgccaa agcgttcgac cagttcggtg cagcagttgt gaatcgattt
     6361 cgctttatta cggtaaaaat tgatcttgcc gatctgtttt tccaactctg gtaatggctg
     6421 cttggcgtag tccgcggcag tgcggttggt tttgaaaagt tccggcgtga tttttgtaac
     6481 cagatcgtca cgtgcttgcg ccgccaggat cagcgcgatg agcagttcca gtggactggt
     6541 aaagtcgaga tcgagtttgg cgtcggggtg ggttttttcc agccgagcga cgattttttt
     6601 taatcggtcc gatgtggcga gtcctgggga atttggcttt ttttgggtca ttttggtttc
     6661 acggaaaaat atggtagcaa aaagcgattc acaaagggag gaatttctcg atggatgcga
     6721 ccgcattgac gactgccaaa caaaacattc gccagcttaa gaaacaaaaa gccgacctcg
     6781 ccgccgccac gcctgatcgt aagaaaacgc gcgttttgca aagaaagatc cggctgctca
     6841 aacgcacgac ccgcgttctc gctggggaaa agaaggcgct tgcggccaag gctgctgccg
     6901 acgccgcctc caaggaggcc gcggaaaaag ccgcagctgc gaaggccgcc gctgatgcta
     6961 aagctgccga agccaaagcc gctgccgatg cggccaaggc tggggaagct gcggacgcca
     7021 gcgcctaggt tgaattgcta tgaatttgcc gcctcctctg cgcggccgcg gttcggcgac
     7081 caatccgaaa aaccgtttcg agtcgatcga gcgggtgccc gagccgccag aggatagcga
     7141 cgaagtctca gcgccgcaca ctcagttttt ccccgacagt tcgaaatcgc tgatcgcctt
     7201 caacaacagc cccgatgtcg gcttcgatgc cagcatcaat ccctatcgcg gctgcgagca
     7261 cggctgcgtt tactgctatg cgcggccgac gcacgagtat ttgggttttt ccgcggggct
     7321 cgatttcgag actaaaatta tggtcaagga ggacgcgccg gaactgttgc gcaaagaact
     7381 agcgcgtccg aaatggcagc cgcagttggt cgcgctgagc ggcaacaccg attgttatca
     7441 gccggtggag aaacacaaac agctcacccg gcgctgcctg gaggtgttcc tcgaattccg
     7501 caatccggtg gtcattatca cgaaaaatca cttggtcacc cgcgacatcg atatcctcgc
     7561 ccaactggct aagttcgact gcattgccgt gacgatttcg gtctcgacat tagatgccaa
     7621 gttggcagcg tcgatggaac cccgggcgtc gcggccgtcg cgccggctgg acgccatccg
     7681 ccaactcgcc gacgcgggcg tgccggtcgg ctatctgcaa gcgccgatga ttcccggcct
     7741 taccgatgcc gaagcccccg cgatcgcgat tgccgcggcg cgcgccggcg cgacgttctc
     7801 gggctatgtt gcattgcggt tgcctttcgc ggtcaaggaa ctattcgagc aatggctcac
     7861 gcagcattat ccggacaaga aggacaaggt tctgaaccga gtccgcgaga tccgcggcgg
     7921 taaactcaac gaccccaact ttgaatcgcg catgcgcggc gaaggaaaat ttgccgagca
     7981 aatggcggcc ttgtttcaac ttgcccgcaa gaagtcgggt atcaccgatc gctggccgaa
     8041 gctgacgaca gaacattttc gccggccaga gaaggatcaa ttgaggttgt tttagtcgcg
     8101 agatcccaat cacgcggtga gacagagaat caacgacgtg aagaacggag atgaaatacg
     8161 ttaagcgtcc gagatctctg cgtcgaggct cggtaagaat gctaatttat tagtcgcagt
     8221 agtattccag ttgataaccc tcggggtcgg ttatgtagaa ggcggtcttg ccgcggcggc
     8281 cgtcgatctt gatatttgat ttattgaat
//
LOCUS       DATLCH010000146         8332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9905143, whole genome shotgun sequence.
ACCESSION   DATLCH010000146 DATLCH010000000
VERSION     DATLCH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9905143"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VMZ02_04290"
     CDS             <1..595
                     /locus_tag="VMZ02_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71180.1"
                     /translation="VKSGCKYIQIDAPGYTAYVDKLSLDRMRARGEDPDKNFQRSVDA
                     DNALIEGFPGVTFGIHICRGNARTIDPTTGKLVPQWHREGSYDAIAEKLFNTLRHHRL
                     LLEYDSDRAGGFEPLRLVPKDKIVVLGLVSTKNSDMETIDDLKRRIDQASKYLPLEQL
                     ALSPQCGFGGIDSKVLSEAEMWMKLDTIVETARQVWT"
     gene            complement(620..901)
                     /locus_tag="VMZ02_04295"
     CDS             complement(620..901)
                     /locus_tag="VMZ02_04295"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HUR71181.1"
                     /translation="MSDGISKYKIGDAVMVASENPSGNPRTPRYIRGKRGVIGLVHGV
                     LENIRDHRGIHKPLYTVRFELSELSSGHDTDTIWVDVHEEWLSSVRPDS"
     gene            complement(910..1983)
                     /locus_tag="VMZ02_04300"
     CDS             complement(910..1983)
                     /locus_tag="VMZ02_04300"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HUR71182.1"
                     /translation="MSGKHGHKPFEGHMHDYMAHVKAVEEDLEYYRAKRFEPRIFYLL
                     RRNVVTFYDLLKVRSTLKKTDHFFRIAKLQGKNIEARVRHLEEWLDEYVKGIALVSAR
                     TVEGMDMVTEDNRKERGDYDDFFKIKKKEHHGTLEERMVNLQQDLADYTELLEVFVQA
                     LLRRGWATREDLAQRWKQLHEERPWAGGVIVAKAWTDPEFKQALLATGREALREMGVH
                     QGKVGKLVVVENTEHTHNVIVCTLCSCYPYDILGDTPWWYKHESYRTNIVKNPRACVK
                     DMFQLELPSDKELRVWDSTSDVRYFVLPQRPAGTYGMSETELAKLVTVDSLIGAGYAL
                     DPSQLREREAAHVAAEIPRVRPD"
     gene            complement(2009..3724)
                     /locus_tag="VMZ02_04305"
     CDS             complement(2009..3724)
                     /locus_tag="VMZ02_04305"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HUR71183.1"
                     /translation="MTDPYSDAKPRIAVFSGPTATIQNSEPLVTSNKAREKYGLPLRT
                     NKDGSPMRFDVLRAQRLAAPVTIYVEQFSAHPLERDHAELYAPPDGYVDRTGVFQKQR
                     TSATDTPVYEITLKPEDGLYPLPYMARQNNGQPWETDGTEKNISAERVRVPFFPDGSR
                     LFEEIDRLGISDEGLGCLLTAKADFDFYRALPSGGYAQGRREGERTDVGAGDIPPEIR
                     GTDFFPYRPGYLRKEPPMAALARVTNVVQRALGTAKYSGGIWLEGSPFVEETIYWLNL
                     LIDTTVPIVGNSSQRPHGAIGNDGDRNIVDSVDYITSKIWADRDGRDCIGAVAILDEQ
                     IFTSRDVQKADARPGGYIATGGHGGIIGRMGHPGTPQFSFKPIKRHTHHSLVNLRQLP
                     MEVQGSRLQDSTIRTIAVSIKDAVGDLLASAIPKVTIVKHARYLPEDTSGDAGGEVDI
                     LARIENNLQIAPHAAFVAEGSAPFGAMSNSVDAALKRATFSGMPVVKVGRGNAGGFVD
                     AKRDPLAVAGSNLTATKARVLIMACLLKLGCLPPAVDPGKPSEAEIDAIKAKLKKFQE
                     IFDTH"
     gene            complement(3753..4331)
                     /locus_tag="VMZ02_04310"
     CDS             complement(3753..4331)
                     /locus_tag="VMZ02_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71184.1"
                     /translation="MSAVQKFIASARELHAQESDPVKRWEKMTPLLQELLSDAEVKEQ
                     SKHWPDCSQGGERAENLLFYEDPDYKFVINGLIKAPHTRTSIHDHAHNWTLYGVLDGS
                     ESIERYERLDDGSKSDYAEVRNTGSVKVSAGKIDLVPPYAIHAEESGDERTVAIIVRA
                     EKAGGFLQGRYDSATNKYWQGYGPKQLPFELK"
     gene            complement(4505..5773)
                     /gene="ahcY"
                     /locus_tag="VMZ02_04315"
     CDS             complement(4505..5773)
                     /gene="ahcY"
                     /locus_tag="VMZ02_04315"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HUR71185.1"
                     /translation="MAGKQYDVKDMRLADAGRKRILWAGQDMPVLQRVKERFAKEKPL
                     RGMRFSACLHVTAETANLVQALKAGGANVLLCASNPLSTQDDVAASLVKHDGIATYAI
                     KGEDNKTYYRHLRAALDHKPVITMDDGADLVSLLHTGYTHLTSNLVASMEETTTGVIR
                     LRALERDGALKIPVIAVNDAATKHLFDNRYGTGQSTIDGVIRATDMLIAGKRVVVAGY
                     GWCGKGVASRARGMGAQVIVTEVDPVRALEAAMDGLEVMTMKEAARIGDLFITLTGDM
                     HVIAEEHLKLMKSGAVICNSGHFDIEIDIKALTKMATRVEKNVRNFVDAYVLPGGRRI
                     YLIGEGRLVNLAAAEGHPASVMDMSFATQALASEWAVMKQKSLTPKVYEVPVEVENWV
                     AKLKLSSMGIRIDALTKAQQRYLTSWDHGT"
     gene            complement(5927..7105)
                     /gene="metK"
                     /locus_tag="VMZ02_04320"
     CDS             complement(5927..7105)
                     /gene="metK"
                     /locus_tag="VMZ02_04320"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HUR71186.1"
                     /translation="MAGKNFLFTSESVSEGHPDKLCDQVSDAILDAHLAGDPDSRVAC
                     ESLAKTGMVVVAGEITSKVKVPYVDIVREVVRDIGYTDSSMGFDGNTCAILTAVEQQS
                     PDISQGVTEGQGLHKEQGAGDQGMMFGYACDETPELMPFPIQMAHSLVHYLAKVRKGG
                     EAYFLRPDSKSQVTVEYRDGKPVKVKNVVISTQHSADVDYAKLRETIIDGVIKAVIPA
                     EYLDKETVFHVNPTGRFVVGGPQGDCGLTGRKIIVDTYGGMGRHGGGAFSGKDPSKVD
                     RSAAYMARYVAKNIVAAELAQRCEVQLAYVIGVAQPVSVLVDTFGTAKIPEDRIAKIV
                     QESFDLSPKGIIQTLDLKRPIYRATAAYGHFGRAPEEGMFTWEKTDRAADLKKAAGRV
                     "
     gene            complement(7185..7460)
                     /locus_tag="VMZ02_04325"
     CDS             complement(7185..7460)
                     /locus_tag="VMZ02_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HUR71187.1"
                     /translation="MSKIVKKLEIKNKLGVHARAAALLVQTVNRFSAQITFSADDQST
                     DGRSILGVMSLGVPQGSKIQIEATGDDAEKAVKALESLINARFNESE"
     gene            complement(7462..>8332)
                     /gene="rapZ"
                     /locus_tag="VMZ02_04330"
     CDS             complement(7462..>8332)
                     /gene="rapZ"
                     /locus_tag="VMZ02_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HUR71188.1"
                     /translation="NYNAGYVQDTWRVNKYVTALLGLCTEQERLIGSPNSVMPAPLIP
                     KFIELCQGYQEDIMRIALGVDLRGSQFLRALPEVLSEMRDGGHHVQVLFFDASDDVLL
                     RRFSETRRPHPSAAGGSVLEGISKERKALESIRALADKVIDTSEFNVHQLKREMEQQF
                     CDAPSARRMTLFLTSFGYKFGIPHDTDILFDVRFLPNPYFVNTLRGKTGLDGEVEEYV
                     LGNSETRAFLDRLYALLEFTLPLYEREGKSSLTLGLGCTGGRHRSVTLVEELHKRFGG
                     SLRVHVKHRDIDK"
BASE COUNT         1823 a         2408 c         2175 g         1926 t
ORIGIN      
        1 cgtaaaatcc ggctgcaaat acatccagat cgacgctccc ggctacacgg cctacgtcga
       61 caaactctcg ttggatcgca tgcgcgctcg cggcgaggat cctgacaaaa atttccaacg
      121 ttcggtcgac gccgataatg ctctcatcga aggtttcccc ggggtaacct tcggtataca
      181 tatctgccgc ggtaacgccc gcaccattga tccgactacc ggtaagctcg taccccaatg
      241 gcaccgcgaa ggttcctacg acgccatcgc cgaaaaactt ttcaacacgc tccgtcatca
      301 taggctgctg ttggaatacg acagcgaccg cgccggcggc ttcgaacccc tgcgcttagt
      361 tccgaaagac aagatcgtcg tgctcggctt agtctcgacg aaaaactccg acatggaaac
      421 catcgacgac ttgaaacgtc gtatcgatca agcgagcaaa tacctgccgc tagaacaact
      481 cgctctgagc ccccagtgtg gtttcggcgg catcgactcg aaagtactca gcgaggcgga
      541 aatgtggatg aaactggata cgattgtgga gacggcaagg caagtgtgga cgtaatcgca
      601 ctcacgaatg tgaacgatac taactatccg gtctcacact actcaaccac tcctcatgta
      661 catcgaccca aatcgtatca gtatcgtggc cgcttgacag ttcgctcaat tcaaatctga
      721 ccgtgtagag cggcttgtgg atgcctcggt gatcgcgaat gttttccaag acgccgtgga
      781 ccagcccaat gacgccgcgt ttcccgcgga tgtagcgcgg ggtacgagga ttaccgctag
      841 gattttcgct agcgaccata acggcatcgc caattttgta tttgctaatc ccgtcgctca
      901 cgacatttct caatccggtc gtacccgggg aatttccgct gcgacgtgag cggcttctcg
      961 ctcgcgcaat tgcgacgggt ccaaggcata gccggcgccg atcaaactat cgacggtgac
     1021 cagtttggcc aattccgttt ccgacatgcc gtacgtgccc gcagggcgtt gcggcaggac
     1081 aaagtagcga acgtcggagg tgctgtccca aactcgtaat tctttatcgc tggggagttc
     1141 cagttggaac atatccttga cgcaggcgcg cgggtttttc acgatattcg tgcgataact
     1201 ctcatgcttg taccaccagg gggtatcgcc aaggatatcg tacgggtagc aagaacataa
     1261 tgtgcaaacg atcacattgt gggtgtgttc ggtgttttca acgacgacca atttgccgac
     1321 cttgccttgg tgcacgccca tttctctgag cgcctcccgg ccggtggcga gcaacgcctg
     1381 tttaaactcg ggatcggtcc acgctttggc gacgatcaca ccgcccgccc agggccgctc
     1441 ttcgtgtagt tgcttccagc gctgcgcgag gtcttcgcgc gtcgcccaac cgcgccggag
     1501 cagagcttgg acaaatactt caagcagctc cgtataatcg gccaagtctt gctgcaggtt
     1561 gaccatgcgc tcttcgagcg tgccatgatg ctctttcttt ttgatcttga aaaaatcgtc
     1621 gtagtcgccg cgctccttgc gattgtcctc ggtgaccatg tccatgcctt caaccgtacg
     1681 tgcggacact aacgcgatgc ccttgacata ttcatcgagc cactcttcga ggtgacgcac
     1741 gcgggcttcg atattctttc cttgcagctt ggcgattctg aagaaatgat ctgtcttctt
     1801 gagtgtcgaa cgcactttga ggagatcgta aaaggtcact acgttgcgcc gaagcaaata
     1861 aaaaattcgc ggctcgaagc gtttggcacg gtaatattcc aagtcctcct cgaccgcctt
     1921 aacatgcgcc atgtaatcgt gcatgtggcc ttcgaagggc ttatgcccat gtttccctga
     1981 catctggggt cctccgcgta acttcgcttc agtgtgtgtc gaagatttcc tggaactttt
     2041 tcaactttgc tttgatcgca tcgatctccg cttcgctcgg cttacccgga tccacggctg
     2101 gcggtaagca acccaatttg agtaaacacg ccattattaa cacccgtgct ttggtcgcgg
     2161 tcagattgct gccggcaacc gccagcgggt cacgcttggc atcgacaaaa ccgccggcat
     2221 tgccacggcc aactttgacc acaggcatac cgctgaatgt agcgcgcttt agcgccgcat
     2281 cgactgagtt gctcatggcg ccaaacggcg cggaaccttc ggcgacaaaa gcggcgtgcg
     2341 gcgctatttg taaattattt tctatacgcg cgagaatatc gacttcacca ccggcatcac
     2401 cagaagtatc ctccggaagg taacgcgcat gcttgacgat cgtcaccttc ggaatcgccg
     2461 atgccagcaa atcaccaacc gcatccttga tcgacactgc gatggtccgg attgttgaat
     2521 cttgaagcct tgaaccttga acctccatcg gcaactgtcg cagattcacc agcgaatgat
     2581 gggtatgccg tttgatcggc ttgaacgaaa attgcggagt accgggatgg cccattctgc
     2641 cgatgatccc gccgtgcccg ccggttgcaa tgtagccgcc tgggcgagcg tcggcttttt
     2701 gcacgtcgcg cgacgtaaaa atctgctcgt cgagaatcgc taccgcgccg atgcagtctc
     2761 ggccatctcg atcggcccaa atcttcgatg taatgtaatc caccgagtcg acgatattac
     2821 gatcgccgtc gttaccaata gcaccatggg gccgttgcga ggaatttccg acgatcggca
     2881 ctgtcgtatc gatcaataaa ttgagccaat aaatcgtctc ttcgacaaat gggctgcctt
     2941 cgagccagat gccgccgctg tacttggcgg tgccgagcgc tcgttggacc acattggtca
     3001 cacgcgccaa ggcagccata ggcggttcct tacgcagata ccctgggcgg tagggaaaga
     3061 aatccgtgcc gcgaatttct ggcggaatat cccctgcgcc gacatcggtc cgttcacctt
     3121 cgcgccgtcc ttgcgcgtaa ccccctgagg gcaaggcgcg gtaaaaatcg aagtccgctt
     3181 tggccgtcag caggcaaccg aggccttcgt cggaaatgcc taaacgatcg atttcttcga
     3241 acaatcgtga tccgtccggg aaaaatggca cccgaacccg ctccgcggaa atatttttct
     3301 ccgtgccgtc cgtttcccaa ggctggccgt tattttgacg cgccatgtac ggcagtggat
     3361 agagaccatc ttccggttta agggtgattt cgtaaaccgg ggtgtcagtg gcgctggtgc
     3421 gctgcttctg aaaaactccg gtgcgatcca catagccgtc cggcggtgcg taaagttcgg
     3481 cgtggtcacg ttccagaggg tgcgcactga attgctcgac ataaatcgtg acgggcgccg
     3541 ccaagcgctg cgcacgcaac acgtcgaaac gcatcgggga gccatctttg ttggtgcgca
     3601 gcggcaagcc gtacttttcc cgcgccttgt tgctggtcac caatggttcg ctattttgaa
     3661 tcgtcgccgt cggtccggaa aataccgcga tgcgcggttt ggcgtcgctg tacgggtcag
     3721 tcatcgtcaa ctttcaaaac gagaaatgaa attcacttaa gctcgaacgg caactgcttg
     3781 ggtccgtaac cctgccaata cttgtttgtc gccgaatcgt agcgtccttg aagaaaaccg
     3841 ccagcctttt cggcgcgtac gatgatcgcc acagtccgtt cgtcaccgct ctcctcggcg
     3901 tgtatcgcat agggcggaac caagtcaatc ttgccggcgc tgacttttac actcccagta
     3961 ttgcgaacct cggcgtagtc ggacttagag ccatcgtcga gtcgctcgta tcgctcgatc
     4021 gattcggagc catcgagcac tccgtaaagc gtccagttgt gcgcatgatc gtggatcgac
     4081 gtgcgcgtgt gcggcgcctt gatcaggcca ttgataacga acttgtaatc gggatcctca
     4141 taaaaaagta aattttcggc acgctcgccg ccctgggagc aatccggcca gtgtttggat
     4201 tgttctttca cttcggcatc ggagagaagt tcctgtagga gcggtgtcat tttctcccaa
     4261 cgttttactg gatcgctttc ctgggcgtga agctcgcgcg cggacgcgat aaatttttgc
     4321 acagcagaca taagaacctc ctgcggcata acaccattgt gacaagttaa cgattattaa
     4381 tccgcggaca cgctggtcgt caagcttgct taaggctgca aattccagat cgcaaccgaa
     4441 taacgcaaaa ggctccgaca gcgaatgacc gccggagcct tttatccacc gtcagcgcgc
     4501 cgatttatgt gccgtggtcc caagacgtta gatacctttg ctgggctttg gtcaaggcgt
     4561 cgatgcggat gcccatcgag ctgagtttca gtttagcgac ccagttctcg acttcgacgg
     4621 ggacttcata aaccttcgga gtgagagatt tctgtttcat gacggcccac tccgaggcca
     4681 atgcctgggt agcaaaactc atgtccataa ccgaagcggg gtgaccctcg gcagctgcca
     4741 aattgaccaa acggccttcg ccgataagat aaatccgccg gccgcccggc aacacgtaag
     4801 cgtcgacaaa attgcgaacg ttcttctcga ctcgcgtggc cattttggtt agtgccttga
     4861 tatcgatctc gatatcgaaa tggccggagt tacagatcac cgcgccgctt ttcatgagtt
     4921 ttagatgctc ttcggcaatg acatgcatat cgccagtcaa ggtgatgaaa agatcaccga
     4981 tgcgggccgc ctctttcatg gtcatcactt ccaaaccatc catcgccgct tccaaagcac
     5041 gaaccgggtc cacttcggtg acaatcactt gagcgcccat gccgcgcgcg cgcgaggcta
     5101 cgcccttacc gcaccacccg tagccggcga caaccacgcg tttgccggca atgagcatat
     5161 cagtagcgcg aatcacgccg tctatggtcg actgccccgt accataacgg ttgtcaaata
     5221 gatgcttggt cgccgcgtcg ttgaccgcta tgacgggaat tttgagcgcc ccatctcttt
     5281 ccagcgcgcg caaacgaatc accccggttg tggtttcttc catgctcgcc accaggttgg
     5341 aggtaagatg tgtgtagccg gtgtgcagca acgacaccag atcggcacca tcatccatcg
     5401 tgatcactgg cttatgatcg agcgcggcac gtagatggcg atagtaggtc ttgttatctt
     5461 cgcctttgat tgcgtaggtg gcgatcccat cgtgttttac cagcgacgcc gccacatcat
     5521 cttgcgtcga tagcggattg gaggcgcaga gcaagacgtt cgcaccgccg gccttgaggg
     5581 cctgaactag attcgcagtc tccgctgtga catgtaagca agccgagaaa cgcatgcctc
     5641 gcagtggttt ctccttggca aagcgctctt ttacccgctg caacaccggc atgtcttgtc
     5701 cggcccagag aatccgctta cggccggcat cggccaagcg catatctttg acgtcatact
     5761 gctttcccgc cattttatcc gtttcctctc atcggttgtc gtatataaat cctgcagaat
     5821 taattcgcct tactcttggt gcatcacagg gtgcgcaagg cacatcctgc tcgacatcgc
     5881 ggatagggcg tgaaaaatga tccgccggcg caaggcaacg aaaatactaa acccggccgg
     5941 cggctttttt gagatcagct gcgcgatcag tcttttccca ggtgaacata ccttcttccg
     6001 gagcgcggcc aaaatggccg taggcagccg tggcgcgata aatgggccgc ttcaaatcga
     6061 gagtttgaat aatgcccttc gggctaagat caaagctctc ttggacgatt ttagcgatcc
     6121 tatcttcggg aattttcgca gtgccgaagg tatcgaccag aaccgaaacc ggttgcgcaa
     6181 cgccaatgac gtaggcgagc tgcacttcgc aacgttgagc gagttccgcg gcaacgatgt
     6241 ttttggcaac gtaacgcgcc atataagccg cgctgcggtc gaccttggac ggatccttgc
     6301 cggaaaatgc gccgccccca tggcggccca tgccgccata ggtatcgacg atgattttgc
     6361 gaccggtcaa accgcaatcg ccttgcggcc cgccgactac gaatcgcccc gtcgggttca
     6421 cgtgaaagac ggtctctttg tccagatatt cggccggtat taccgctttg ataacaccgt
     6481 cgataatggt ttcgcgtagt ttcgcatagt cgacgtccgc gctgtgctgc gtcgagatca
     6541 cgacgttttt aaccttcacc ggcttgccgt cgcgatactc gacggtgacc tgggatttac
     6601 tgtcgggacg gagaaaataa gcctcgccgc ctttacgaac tttggcgaga tagtgaacca
     6661 ggctatgcgc catctggatc ggaaaaggca tcaattccgg tgtttcgtcg cacgcataac
     6721 cgaacatcat gccttgatcg ccggcgccct gctccttgtg caaaccctgc ccctcggtga
     6781 cgccctgcga gatatccggc gactgctgtt ccaccgcggt gagaatagcg catgtattgc
     6841 cgtcaaatcc catggacgaa tccgtgtaac cgatgtcgcg cacgacctct cgtacaatgt
     6901 cgacataagg aaccttgacc ttgctggtga tttcgccggc aaccacgacc atgccggtct
     6961 tagctaaact ttcacacgca acacggctgt ccgggtcgcc ggcaaggtga gcgtctagaa
     7021 tcgcatcgga tacttgatcg caaagtttgt cggggtgccc ttctgaaacg gactctgagg
     7081 taaataagaa gtttttgccg gccatcaaaa agacctccaa acgtttccaa acagatcaga
     7141 atttaatctg cgcatactat cggatcagac gtggcggttc aaccttattc actctcgttg
     7201 aagcgcgcgt tgatgagact ttcgagcgct tttaccgcct tctccgcatc atcgccagtg
     7261 gcttcaatct gaatcttgct tccctgcggc acccccaacg acatcacacc taaaatgcta
     7321 cggccatcgg tgctttggtc atccgccgag aacgtaatct gggcagaaaa cctgttgacc
     7381 gtctgcacga gcagggcggc tgcgcgagcg tgaactccta gcttgttttt gatctcgagc
     7441 ttcttaacga ttttactcat gttatttatc gatgtcccga tgcttaacat ggacacgtaa
     7501 gctgccgcca aatcgcttgt gcaactcttc gaccaacgtt acggatcgat gtcgccctcc
     7561 ggtgcacccg aggccgagag tcaaactgct cttcccctcg cgctcgtaca gcggcaaggt
     7621 aaactccaag agcgcataaa gccggtcgag aaaggcgcgg gtctctgaat taccgagcac
     7681 gtattcctcg acttccccat cgagaccggt cttgccgcgc aaagtattaa cgaaataggg
     7741 attgggcagg aaacgcacat cgaagagaat atccgtgtcg tgggggatcc cgaacttgta
     7801 gccgaaagag gtcagaaaca gcgtcatgcg ccgcgccgac ggagcatcgc aaaattgctg
     7861 ttccatctcg cgcttgagtt gatgcacgtt gaactcgctg gtatcgataa ctttgtcggc
     7921 cagagcgcga atcgactcca acgctttgcg ttcttttgag atgccttcca gaacagaccc
     7981 accggcagcg gagggatgag gccgccgcgt ttcactgaag cggcgcaaca gcacatcgtc
     8041 ggaggcatcg aaaaatagca cttgaacatg atgcccgccg tcgcgcatct cgctcaggac
     8101 ctcgggtaac gcccgcaaaa actgactgcc gcgcaaatcg acacccagcg caatgcgcat
     8161 gatatcttcc tggtagcctt ggcaaagctc gatgaatttc ggaatcagcg gcgccggcat
     8221 aactgagttc ggacttccga tcagtctttc ctgttcggtg cacaggccca gtagcgcggt
     8281 cacgtatttg ttaacacgcc acgtgtcctg aacatagcct gcgttgtagt tt
//
LOCUS       DATLCH010000147         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1173380, whole genome shotgun sequence.
ACCESSION   DATLCH010000147 DATLCH010000000
VERSION     DATLCH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173380"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VMZ02_04335"
     CDS             <1..225
                     /locus_tag="VMZ02_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71189.1"
                     /translation="AIQFGDSAHSIAIISSIGVFFRTLAVGGVFVYRSIIVAPSNSPK
                     FIARTQFPLARSDSVLGPKGCLTGIGFADA"
     gene            306..614
                     /locus_tag="VMZ02_04340"
     CDS             306..614
                     /locus_tag="VMZ02_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HUR71190.1"
                     /translation="MIERQQPYARQLHVCINNRNGESKSCGYDGSDEVVEELRKVTKK
                     RNLKGKVRVVRSGCLDVCAFGPNMMIWPEGLWYMKVTKEDVPQIVDKYLSLEAADQAK
                     "
     gene            815..1579
                     /locus_tag="VMZ02_04345"
     CDS             815..1579
                     /locus_tag="VMZ02_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71191.1"
                     /translation="MADKSLTLDQYRTLRKDNWWVEPLLVVVVLGGFAIYATWAALQN
                     ANYYAAPYLSPFYSPCISANCAHVTLPLVGSWWNLSPAFLILWVPGGFRATCYYYRKA
                     YYRSFFQSPPACAVRDAAKNYSGEARFPFVLQNIHRYFFYLSLVILAFLWWDALLAFR
                     FADGFGMGVGTIVLCINAVLLSLFSLSCNSCRHVCGGYLNSFHSAPSKYKTWSFISRL
                     NEKHMEYAWVSLIWVALTDLYVRLLAMGVIADLRFF"
     gene            1579..3381
                     /locus_tag="VMZ02_04350"
     CDS             1579..3381
                     /locus_tag="VMZ02_04350"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HUR71192.1"
                     /translation="MAQDKYETHEYDVVVIGAGGAGLRAAVEASAQGAKTALVCKSLL
                     GKAHTVMAEGGIAAAMGNVYPEDNWKVHFRDTMRGGKLLNNWRMAQIHAQESPDRVLE
                     LEEWGALFDRTKEGLILQRDFGGHRYARLAHVGDRTGLEMIRTLQQHAVHRGIDVYME
                     CNLQRLLTDDGRISGAFGYWRESGKFIMFKCKAVVLATGGGGKAWKVTSNSWEYTGDG
                     VTMAADAGADLMDMEFMQFHPTGMVWPPSVRGILVTEGVRGDGGTLKNTKNERFMFNY
                     IPDFFKAETAETIEEAERWYEDKRNNRRTPDLLPRDEVARAINSEVKAGRGTPHGGVY
                     LDIATRRPAEYIRKRLPSMYHQFKELADVDITKEAMEVGPTCHYSMGGVRVDADSTAT
                     TVPGLFAAGEVAAGLHGANRLGGNSLSDLLVFGRRAGLYAAEYAKGMSGSLSVNNDQV
                     ESFARDMLAPFESKGNENPYKVHSDLQECMQDLVGIIRVESELNQALEIIQKLKQRSK
                     DLRVEGNRHYNPGWHLALDLHSLLTFAEAATLAAIIRKESRGGHTRDDYPNPDPKFAK
                     VNIITRMRNGEMTQTQEPLPEMPEELKALLEETK"
     gene            3381..>3882
                     /locus_tag="VMZ02_04355"
     CDS             3381..>3882
                     /locus_tag="VMZ02_04355"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HUR71193.1"
                     /translation="MAEQQVHMRVWRGDASGGEFKDYRIAVGEGQVVLDVIHAIQAQQ
                     ANDMAVRWNCKAGKCGSCSAEVNGKPRLMCMTRMNTFEPGETITVSPMRTFPIIRDLV
                     TDVSYNYQKAKEIPAFKPRPKESDGTRRMYQEDIDRVQEFHKCIECFLCQNVCHVIRD
                     HDENKPV"
BASE COUNT          905 a         1050 c         1062 g          865 t
ORIGIN      
        1 gcgattcagt tcggcgacag cgctcatagc attgcaatca ttagttcgat tggagtgttt
       61 ttccggacgc tagcagtcgg tggtgttttt gtctatcgca gcattatagt tgctccatcc
      121 aattcaccta agtttatcgc cagaacgcaa tttccgcttg cacgcagtga cagcgttctc
      181 ggccccaaag gctgcttgac cggtatcggt tttgccgatg cgtaaccggg gtctgcatct
      241 ccgacccgcg tgcaatggta aaatatttct gctataaaat tcgccaatgc ctccaaggag
      301 ttcccatgat cgaacgacag cagccgtatg cccgccaact gcacgtgtgc atcaataacc
      361 gtaacggcga gagtaaatcc tgtggctacg acggatctga cgaagtggtg gaagaactga
      421 gaaaagtcac gaaaaaacga aacctcaaag gcaaagtccg ggttgtcaga agcggttgct
      481 tggatgtctg cgccttcggc cccaacatga tgatctggcc ggaaggttta tggtacatga
      541 aagtcaccaa agaagatgtg ccacagatcg tcgacaagta cttgagcctc gaagccgcag
      601 accaggcgaa gtaaaccaag acgcatgcga tattcgcgct gacccattcc tgatccaagc
      661 ttagttactt acatggagcg cggctttgcc caacggtgaa gccatgcttg tgttccaact
      721 cggcttgaat tctttgcgcc gttggcagat tacttgacat tgaaccgatg taataaaatt
      781 cgcacaaatc aaagagcaaa aaggaacgat catcgtggct gataaaagtt taactctcga
      841 tcaatatcgc acattgcgga aggataactg gtgggtcgag ccgctgctcg tggtcgtggt
      901 gttgggcggg ttcgctatat atgccacttg ggctgccctg caaaacgcca actactatgc
      961 tgcaccttac ctttcaccct tttattcgcc gtgtatttcg gccaactgtg ctcatgtcac
     1021 tctgccgctc gtaggttcct ggtggaattt gtcgccggcc ttcttgattc tctgggtgcc
     1081 cggcggcttt cgcgcgactt gttattatta tcgcaaagcc tattaccgct ctttcttcca
     1141 atcccctcca gcttgcgccg tgcgcgacgc ggccaagaac tacagcggtg aagcccgctt
     1201 ccctttcgtg ctgcaaaata tccatcgtta ctttttttat ctttcattgg tgattttggc
     1261 gtttctttgg tgggatgcgt tgctggcatt ccgctttgcg gacggtttcg ggatgggcgt
     1321 gggtaccatc gtgctgtgca tcaatgccgt gcttttgtct ctgttcagcc tctcgtgcaa
     1381 ctcgtgtcga catgtttgcg gcggctattt gaattcgttt cacagcgccc cgagtaaata
     1441 caaaacgtgg tctttcatca gccggctcaa tgaaaaacat atggaatacg cctgggtcag
     1501 tttgatctgg gtcgcgttga ccgatctcta tgtacggctg ttggcgatgg gcgtgatagc
     1561 cgatttgagg tttttctaat ggctcaagac aaatacgaaa cccatgaata cgacgtcgtg
     1621 gtcatcggcg ccgggggcgc aggtttgcgc gccgcggtcg aagcctcggc acagggcgct
     1681 aaaaccgctt tggtttgcaa atccctgctc ggtaaagccc ataccgtcat ggcggaaggc
     1741 ggcatcgccg cggcaatggg caatgtctat cctgaagata attggaaggt ccactttcgc
     1801 gataccatgc gcggcggcaa gctgctcaac aactggcgca tggcacagat ccatgctcag
     1861 gaatcacccg atcgcgtgct tgagttggag gaatggggcg cactcttcga ccgcaccaaa
     1921 gaaggtttga ttttacagcg cgatttcggc ggccaccgtt acgcgcgcct cgcccatgtc
     1981 ggcgatcgca cgggcctcga aatgatccgt actctgcaac agcacgccgt gcacagaggc
     2041 atcgacgttt atatggagtg caacttgcag cggcttttga ctgacgacgg ccgcatcagc
     2101 ggcgccttcg gctactggcg cgaaagcggc aagttcatca tgttcaagtg taaggcggtt
     2161 gtgctcgcca ctggcggcgg cggcaaggct tggaaggtca catcgaattc ctgggaatac
     2221 acgggggatg gcgtcaccat ggccgccgat gccggcgccg atctgatgga catggagttc
     2281 atgcaatttc atcctaccgg catggtgtgg ccacccagcg tgcgcggcat tctggtgaca
     2341 gaaggcgtgc gcggcgacgg cggcacgttg aagaacacca aaaacgagcg cttcatgttc
     2401 aactacatcc cggatttctt caaagcggaa accgccgaga ccatagaaga agccgaacgc
     2461 tggtacgaag acaaacgcaa caatcgccgc acgcccgatc tattgccgcg cgacgaagta
     2521 gcccgagcga tcaactcgga agtcaaagct ggccgcggca ctccccatgg cggtgtgtat
     2581 ctcgatatcg ccacgcgccg gccggccgag tacatccgca aacggcttcc ttccatgtac
     2641 catcaattca aagaactggc ggatgtcgac attaccaagg aggcgatgga agtcggccct
     2701 acctgccatt actcgatggg cggcgttcgc gtcgacgctg acagcaccgc gaccacagtg
     2761 cccgggttgt ttgctgccgg cgaagttgcc gccggtctcc acggcgccaa ccgcttgggc
     2821 ggcaactcgc tatccgattt gttggttttt ggaagacgcg ccggtctcta tgcggcggag
     2881 tatgccaaag gaatgagcgg atcgctgagc gtgaataacg atcaagtcga atcattcgcg
     2941 cgcgatatgc tcgcgccctt cgaaagtaaa ggcaacgaga atccctataa ggttcactcc
     3001 gacttgcagg aatgcatgca ggacttggtc ggcatcattc gcgtcgagtc ggagctaaac
     3061 caagctcttg aaattattca aaagcttaaa caacgctcca aggacctgag agtcgaaggc
     3121 aatcgtcact acaatccggg ttggcatctg gcgttggatc tgcactcgtt gttgaccttc
     3181 gctgaagcag ccactctggc agcgatcatc cgcaaagaaa gccgcggcgg ccatacccgc
     3241 gatgactacc ccaatcccga cccaaagttt gccaaagtaa acataatcac tcgcatgaga
     3301 aatggtgaaa tgacgcagac tcaggagcct ctgccggaaa tgcctgagga actaaaagca
     3361 ctgctcgagg agacaaaata atggctgagc aacaggttca tatgcgggtg tggcgcggtg
     3421 acgccagcgg cggtgaattc aaggactacc gcattgccgt gggtgaaggc caagtagtgc
     3481 tcgacgttat tcatgcgatt caggcacagc aagccaacga tatggcggta cgttggaact
     3541 gcaaagcggg gaagtgcggc tcctgcagcg ctgaggtaaa tggcaagccg cgcctgatgt
     3601 gcatgacgcg catgaacacc ttcgagccag gcgaaaccat cacggtctcg ccgatgcgga
     3661 cgttcccgat tatccgcgat ctcgtcacgg acgtctctta caattatcaa aaagcgaaag
     3721 aaattccggc gttcaaaccg agacccaaag aatccgacgg cacccggcga atgtaccagg
     3781 aagacatcga ccgcgtgcaa gaattccaca aatgcatcga gtgcttcctg tgccagaacg
     3841 tctgccatgt tatccgcgac catgatgaaa acaagccggt gt
//
LOCUS       DATLCH010000148         7388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1917268, whole genome shotgun sequence.
ACCESSION   DATLCH010000148 DATLCH010000000
VERSION     DATLCH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917268"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /gene="uvrC"
                     /locus_tag="VMZ02_04360"
     CDS             <1..1382
                     /gene="uvrC"
                     /locus_tag="VMZ02_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HUR71194.1"
                     /translation="CLQYQIKRCMGPCVLPVAADVYREQVRQTMLFIEGRQQELIDEL
                     KQRMSAKSDALEYEAAARIRDQIQAVEKTLEKQRMVAHWGSDQDIFGLYREGGFIEVQ
                     VLLVRQGKLTGSQSYSLTDLEFPDAEIMGSLLTQFYQGQRFVPDEILLPVELEDTAVR
                     EEYLAEHKGKSVKILSPQRGDKRHLVDMAIDNAKQSFADRHDQEKAREKMLHELQNSL
                     RLKNYPHRIECFDISMIHGAHAVGSMVTFIDGEPDKNLYRHFRIKTIGAESGGDDFGM
                     MLEMLKRRFSRGKEEGDLPELIVVDGGKGQLAMALAAMAEVGIDNVEAVGLAKMRVQA
                     APRSTEIERSEERVFLPGQANPVILKRNSNALFLLQRVRDEAHRFAITHHRKLRSQQT
                     LYSALDRISGIGGVRKRALLRAFGSIKRIEEANLEDLLKVPSMNEKLAQQVLDALRRP
                     ELATPQTAE"
     gene            1394..3871
                     /locus_tag="VMZ02_04365"
     CDS             1394..3871
                     /locus_tag="VMZ02_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HUR71195.1"
                     /translation="MAERDEAAGLAKVVFHPSAWLIVPTLFLLLGQAVAAAPWPALSS
                     EWLAVIPCAMMPFYRRWRCWVILGLLVILAFSLGYVRHREILFPEFPEHHLRSVMLRH
                     QQVLIEGQLTIEPESLINRQRWIVRAERIWHPTGAEEISGDLQLSLRNARRDWRYGDR
                     IRFLVRPNLPQNSGNPGGFEYATYLSRRQIYVTGFLENDAEIELLARDPGVIRGAVED
                     IRREIRRYVDATFSSDGGALMKALTVGEMGGIRKEVRNAFTAAGVNHVLSISGLHVAM
                     LGVVIFAAIRYGLSFSNYLLLRFSLLKAATFFAFLAVVFYTALAGGMVPTVRSAIMIG
                     VYQLAVLLDREEEVFASLTLAALLIALVWPGVVADISFQLSFLAVLFIAWGMRNFYRW
                     LARERTQELPQEKGWIKEKARQAAFHLAVPLLATLGTGPLIAHYFGNLSLAGFVTNPL
                     IVPLVGFVVVPLGLTIGLLTFVASEYAWPLAWLGDRLANWTIAIVEYVGRLPLASFRV
                     ASPSLLEVGALYAAVIAFFALRKRSHIAVAAGIFFVLLVGDLYYWRVQRYDSNRLRIT
                     HLNVGQGDAAVVELPGGKVLVIDAGGAAVGEFDTGESIVAPYLRSRKILKIDYLFVSH
                     ARIDHYGGMRALAQDFSPSEFWSGAAKGGTQRFEELEEALEQLNIVRRELTGGLPCRD
                     IATVRLCFLFGPAGDMAESPVVVRLEFGKASFLFASDVDKRDELQLAQHPSGLSSTVV
                     KIPRHGSATASSAEFIAAVTPKLAILSAGARSRTEAKRDEVVERYRAAGAEVLRTYDD
                     GAITLETDGKTISYSGFKSGRAGKLEL"
     gene            complement(4066..5004)
                     /locus_tag="VMZ02_04370"
     CDS             complement(4066..5004)
                     /locus_tag="VMZ02_04370"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71196.1"
                     /translation="MSSTLITGLGLVGTSFAQHAIKRGDTVVFYDVAPRKDFLAHKLG
                     DANVTVVQRDIRDLPSLIESIQKYKCDTVIHTAGLIGGKVGNPIYSGMQINVMGTINV
                     AEAVRLTGVKRLVQISTFGVYDRRQGEPNPIDEGFRRGPGEAYGNSKVAKELMVEAYQ
                     RMFGFELIVLRLANVYGVGHFAGGSGGGEMVQNMLQTGIKGGVVKIAQEVARDFEYVY
                     YKDLGRALDKAVTTPLKEPVTLNIGTGVVIPFDDLVSTAEKLLPNLQVEIIPGVKPRS
                     AKQPMVIAKAKQVLGWAPDYDIETGFKDYISELKAL"
     gene            5161..6255
                     /locus_tag="VMZ02_04375"
     CDS             5161..6255
                     /locus_tag="VMZ02_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71197.1"
                     /translation="MHGVVDADTHIAEPEAMWQLIDEKMAPRRPVLVDLPDDTWFGDR
                     NALWLIDGNIFPKPAGKGSYRLVTPSAQKAEKIRGDIAIASREVANVEARIKDMDRLG
                     VDVQVIYPTLFLVYITDDPELDTALSKAYNSWLGNACAKSNGRLKFVAVLPLRSIPES
                     LKEMKRARDLGAVGIFFRGIEGDKTLDHPYFHPVYEQAAKLGMPITIHTGSGAPWMLP
                     YFEHARNHTFAHGRALPIFAFRDLLANRIPEKFPGLRFGFIEASAGWAPFMIHILRRL
                     FKDRPQYKNSAELFRASNIFIAAEADEDIPYLAKCIGEDNIVIGSDYGHNDPSAEEKL
                     VESLQRREDIPEAMAKKILCDNPRRLYGLQ"
     gene            6383..7090
                     /locus_tag="VMZ02_04380"
     CDS             6383..7090
                     /locus_tag="VMZ02_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71198.1"
                     /translation="MKQIKEYVDRVAHHFKAKLGASLVDAYTLGSLAHGGYSTVYSDI
                     DVGLLINCAEAPVEMAALIADAKTLDDVYGKKLSIFWGNPEFTWGRLPNLDRLDLLDH
                     GVPLLHGYKPQFRRPTQREIHDELLRSIEKSWKPRLPELNSLKELTPEKRKPYIRAIL
                     YAARLVYSWDNLAMESNDRAVEYLHQTEPAGLDLKPIDMALACRQAKCTAEDVFNLHT
                     DLNRQCESAVRYISKRL"
BASE COUNT         1565 a         1993 c         2144 g         1686 t
ORIGIN      
        1 cgtgcttgca atatcaaatc aagcgctgca tggggccctg cgtgctgccg gtggcagccg
       61 atgtttaccg cgagcaggtg cgccagacta tgctgtttat cgaaggccgg cagcaagaac
      121 tcatagacga gttgaagcag cgcatgtcgg cgaagtccga cgccttggaa tacgaggcgg
      181 cggcgcgaat tcgcgaccag attcaagcag tcgaaaaaac cttggagaaa cagcgcatgg
      241 tggcgcactg gggcagcgac caggatatct ttggattgta tcgcgagggc ggtttcatcg
      301 aagtacaggt gctgctagtg cgccagggga agctgaccgg cagccagtcc tattcattga
      361 cagatttgga atttcccgac gcagagatca tgggctcgct cctgacccag ttttatcagg
      421 gccagcgttt cgttcctgac gagatactct tgccggtcga gctcgaggac accgcagtgc
      481 gcgaggagta tctcgccgaa cataagggca agagcgtcaa gattctttct ccacagcgcg
      541 gcgacaagcg ccacttggtc gatatggcga tcgacaacgc caagcaaagt ttcgccgacc
      601 gccatgatca ggagaaagcg cgggagaaaa tgctgcacga gctgcagaac tcgctgcgtt
      661 tgaaaaacta ccctcatcgg atcgaatgtt tcgatatctc gatgatccat ggcgcccatg
      721 ccgtgggctc gatggtgacg ttcatcgacg gcgaaccgga taagaatctt tatcgccact
      781 ttcgcatcaa gaccatcggc gctgagtcgg gcggcgacga tttcggcatg atgctggaga
      841 tgctcaagcg gcgtttcagc cgcggcaagg aagaaggcga tctaccggaa ctgatcgtgg
      901 tcgacggcgg caagggacag ttagccatgg ctttagcagc catggccgag gtcggcatcg
      961 acaatgtcga ggcggtaggc ttggccaaga tgcgtgttca agcggcgccg cgcagcacag
     1021 agatcgagcg ctcggaagag cgcgtattcc tgccgggtca ggccaatcca gtgatcttaa
     1081 aacgcaactc caatgcgcta ttcctgctcc agcgcgtgcg cgacgaggcc catcgcttcg
     1141 ccatcacgca tcatcgaaaa ctgcgttctc agcagacgct ctactccgcc ctcgatagaa
     1201 tttccggtat cggtggcgtg cgcaaacgcg cgctgctgcg cgccttcggc agtatcaagc
     1261 gcatcgaaga agcgaacttg gaggacctac taaaggtgcc gtcaatgaat gaaaagctgg
     1321 cacagcaagt gctcgatgcg ctgcgccgac cggagttagc aacgccgcag accgccgagt
     1381 aagccacacc aacatggcgg agcgggacga ggcagctgga ttggcaaagg tggtgttcca
     1441 cccgtcggcc tggctgatag tcccgacact atttctcctg ctcgggcaag cggtcgctgc
     1501 ggccccttgg ccggcgcttt caagcgaatg gctggccgtc attccgtgtg cgatgatgcc
     1561 gttttaccgg cgctggcgct gctgggtcat cctcggactc ctggtcattt tggccttttc
     1621 gctgggctac gtgcgtcatc gtgaaattct ctttccggaa tttcccgagc accatttgcg
     1681 atcagtgatg ctgcgccacc agcaggtttt gatcgaaggg cagctcacga ttgagccgga
     1741 gagccttatc aatcgccaac gctggatcgt ccgtgccgaa aggatttggc atcctaccgg
     1801 agccgaggag atcagcggtg atctgcagct tagcctgcgc aacgcgcggc gcgactggcg
     1861 ctacggtgac cgtatccgtt ttttggtgcg gcctaacctg ccccagaata gcggcaatcc
     1921 cgggggcttc gagtacgcaa cctatctttc tcgccgacag atctacgtca ccggatttct
     1981 tgaaaatgat gccgaaattg aactgctcgc gcgcgatccg ggcgtgatcc gcggcgcggt
     2041 tgaagatatt cgccgggaaa ttcgccgtta cgtggacgcc actttctcgt ccgatggcgg
     2101 cgcgctgatg aaggctctga cggtgggcga gatgggcggt atacgcaagg aagtccgcaa
     2161 cgcctttacc gccgctggcg ttaaccatgt gttgtcgatt tccggactcc atgtcgccat
     2221 gctgggagtt gtgatcttcg ccgcgatccg ttacggcctg tcgtttagca actatttatt
     2281 gctgcgtttt agtttactca aagcggcaac cttctttgcg tttttggcag tggtatttta
     2341 tacggcgttg gccggcggca tggtgccgac cgtgcgctca gcgattatga tcggcgtcta
     2401 tcaattggct gtgctgctgg atcgggagga ggaggtcttt gccagcttaa ctctggcggc
     2461 gctgttgatc gcattggtct ggcccggtgt agttgccgac atttcgtttc agttgtcgtt
     2521 tcttgcggtc ctgtttatcg cctggggtat gcgtaatttt tataggtggc tggcccggga
     2581 aaggacacaa gagctgcctc aagaaaaagg ctggatcaaa gaaaaagcgc ggcaggcagc
     2641 ctttcatctg gcagtgccct tgctggcgac tctcggtacc ggtccgttaa ttgcgcacta
     2701 cttcggaaat ctgtctctcg ccggcttcgt cactaatccg ttgatcgtgc cgctggtggg
     2761 atttgttgtc gtgcccttgg gtcttactat cggtttattg actttcgtgg caagtgaata
     2821 tgcttggccg ctcgcctggc tcggcgatcg tttggccaat tggaccattg ccatagtcga
     2881 gtatgtcggg cggttaccgc tggcaagctt tcgcgtcgcc tcgccgagtc tactggaggt
     2941 cggcgctctt tatgcggctg tgattgcctt tttcgctctg cgcaagcgca gccatatagc
     3001 agtcgcggcc ggcatctttt ttgtcttgct cgttggtgac ctctattatt ggcgggttca
     3061 gcgttacgac agcaaccgat tgcgcatcac ccatctaaac gtcggccagg gcgatgcggc
     3121 ggtggtcgaa ctgcccggcg gcaaagttct ggtgatcgat gccggcggtg ctgcggtggg
     3181 ggagttcgat accggtgaaa gcatcgtcgc tccttatctg agatcgcgaa aaattctcaa
     3241 aatcgattat ctttttgtga gccatgcgcg catcgatcat tacggcggta tgcgcgctct
     3301 agcccaagat ttttctccca gcgagttctg gtcaggcgct gccaaaggcg gaactcagcg
     3361 gttcgaggag ctcgaagaag cattggaaca actcaatatt gtccggcggg aattgaccgg
     3421 tggcttgcct tgccgcgaca ttgctaccgt gcgcctgtgt ttcttgtttg ggcccgctgg
     3481 cgacatggcc gaaagtccag tggtcgtgcg ccttgaattc ggtaaggcgt cgtttctgtt
     3541 cgccagcgat gtcgacaagc gcgacgagtt gcagctggcg caacatccaa gtggattgag
     3601 cagcactgtg gtaaagattc ctcgccatgg cagcgcgacc gcaagttcag cggaattcat
     3661 cgccgcagtg acacctaaat tggcgatctt gtcagcggga gcaagaagtc gaacggaggc
     3721 aaaacgcgac gaagtggttg agcgttatcg cgcggccggt gctgaggtgc tgcgcacata
     3781 cgatgacggc gcgatcactc tggagactga tgggaagacg ataagttata gcggctttaa
     3841 aagcggcagg gcgggtaagt tagagttgtg attccgcggt gagaaaatca ccacgacatg
     3901 gttcaacaga gaatgatggc aaggaaccct cacatgacgg aacgttcgtg tcaattcgtc
     3961 gcctgatcgg cctgcacaca tattgaagta ggcacgaaga ataccgagtt cgaaggtttg
     4021 gtatactcaa ccgccgtggc cttcgtgcgt tcttagtgag tgagcctaga gcgccttcag
     4081 ttcgctgatg taatccttaa aaccggtttc aatgtcatag tccggagccc aaccgagcac
     4141 ctgtttcgct ttcgcgatca ccatcggctg tttcgccgag cgtggtttga caccggggat
     4201 aatctccacc tgtaaattcg gcagcagttt ttcggcggtt gaaaccagat cgtcgaaagg
     4261 tatgaccaca ccggtgccga tattgagcgt caccggttct ttcagcgggg tcgtgaccgc
     4321 cttgtccaaa gcacggccga gatctttata atagacatat tcaaaatccc gggcgacttc
     4381 ctgggcgatc ttgacgacac cgcctttgat gccggtttgc agcatgttct ggaccatctc
     4441 accgccgccc gacccgccgg caaagtggcc gacgccgtag acgttggcta gccgcagcac
     4501 gattaattcg aagccgaaca ttcgctggta ggcctcgacc atgagttcct tggcgacttt
     4561 cgagttaccg taggcttcgc cgggaccacg gcgaaaccct tcgtcaatcg gattgggttc
     4621 cccctgccgc cggtcgtaga cgccgaaggt gctgatctgc accaagcgtt tcactcctgt
     4681 gagccgcacc gcttcagcga cgttgatcgt gcccatgacg ttgatttgca tcccagaata
     4741 aatcggattg ccgaccttgc cgccgataag tcccgccgtg tgaataacgg tgtcgcactt
     4801 atatttttgg atcgattcaa tgagtgacgg caggtctcga atgtcgcgct gcacgaccgt
     4861 aacgttagcg tcgccgagct tgtgcgccaa aaaatccttg cgcggtgcga cgtcataaaa
     4921 caccacggta tcgccgcgtt taatcgcgtg ctgcgcgaac gatgtaccga ccaatcctaa
     4981 acccgttatc agtgtcgatg acatatcaac tcctttttgt tgattggttt agttcctatt
     5041 gcaaccgctt cgaaatgtct aggggttttt cgatgcctcg tttctcgtcc attgcaatgg
     5101 gagattatcg cggtgtatat cgcactcaga tatccgtcaa aatagaggga ggaaggcaac
     5161 atgcacggtg tagttgatgc cgatacccac atcgccgagc cggaagccat gtggcaattg
     5221 attgacgaga agatggcgcc gcgccggccg gtgttggtcg atttgccgga cgacacctgg
     5281 ttcggcgatc gcaacgcgct ctggctgatc gatggaaata tttttcccaa gccggcggga
     5341 aaggggagct atcggctggt aaccccatcg gcccaaaagg cggaaaaaat tcgcggcgac
     5401 atcgccatcg cgagccgcga agtcgccaac gtcgaggctc gtatcaaaga tatggaccgg
     5461 ctcggcgtcg acgttcaggt gatctatccg acgttattcc tggtctacat caccgatgat
     5521 cccgaactcg ataccgctct cagcaaagcg tacaactcct ggcttggcaa tgcatgcgcg
     5581 aagtccaacg gccggctcaa gtttgtcgcg gtgctgccgc tgcgttcgat cccggagtcg
     5641 ctcaaggaaa tgaagcgggc gagagatctc ggcgcggtgg gcatcttctt ccgcggcatc
     5701 gaaggcgaca aaactttgga tcatccctat tttcatccgg tctatgaaca ggctgccaag
     5761 ctcggcatgc cgatcacaat tcatactggc tcgggcgcgc cctggatgct gccgtatttc
     5821 gaacatgcgc gcaatcacac gttcgcgcat ggccgggcgc tgccgatctt cgcttttcga
     5881 gatctgctcg ccaaccgcat tcccgagaag tttcccgggc tgcgtttcgg cttcatcgag
     5941 gcttcggcgg gctgggcgcc gttcatgatc cacattctgc gtcgcctatt caaagaccgc
     6001 ccgcaataca agaacagcgc cgaactgttc cgtgcatcca atatctttat cgccgccgag
     6061 gccgacgaag acatccctta tctcgcgaaa tgcatcggcg aagataacat cgtcatcggc
     6121 tcagactatg gccacaacga tccgtcggcc gaggagaaat tggtcgagag cctccaaagg
     6181 cgggaagaca ttcccgaagc aatggccaaa aagatactgt gcgacaaccc ccggcgattg
     6241 tacgggctgc agtagagttg agcgggtccg accgcgctgc agtttcgctg atgcgggatg
     6301 ttgtctctcg gcaggtcgta aacatatgcc gtaaattttg tatgtttgcg cgagatattc
     6361 tcaatcgagt tatttgagac tgatgaaaca aatcaaagaa tacgttgacc gagtggcaca
     6421 tcacttcaaa gccaagctag gcgcttcgtt agtagacgct tatacgctcg gcagtctcgc
     6481 tcatggcgga tatagcacgg tttacagcga catcgacgtt gggctgctga tcaactgcgc
     6541 agaagcgccc gtcgaaatgg cggctctgat cgccgatgcg aagacgttgg atgacgttta
     6601 cggtaagaag ctgtcgatct tctggggcaa tcccgaattc acgtggggac ggctgcccaa
     6661 cctcgatcgg ctcgacctcc tggatcacgg cgtgccgctt ctccacggat ataagccgca
     6721 gtttcgccgt ccgacgcagc gagagattca tgacgagttg ctgagaagta tcgaaaaaag
     6781 ttggaagccg cgattgccgg aactcaacag cctgaaagaa ttgacgccgg aaaagcgcaa
     6841 accttacatt cgagcgatcc tttatgccgc gcggttggtt tacagctggg ataatctggc
     6901 gatggaatcc aatgaccgcg cggtcgagta tctccaccaa acagaaccgg cgggtctcga
     6961 tctcaaacca atcgacatgg cgctagcgtg ccgtcaggca aagtgcaccg ctgaggatgt
     7021 gttcaatctc cacactgacc ttaaccggca gtgtgaaagc gccgtcagat atatttcgaa
     7081 aagactgtga gcctgcgccg gccgacgccg caatgtttct cgcgaacgtc gtataactcg
     7141 cttaggtcgt tacgaagaaa gcttgtttga gtgttacccg aagctgcgta aggctagtag
     7201 cttgcatgtt cgggtgaagt tttaggcgtc ccaccgccgg atcatttgcc ggtgtcgaag
     7261 acaatcgaga gttcgaccgc cgagtgacca tcgaaaggcc agatttcgcg gtggttgcaa
     7321 aaaaggaagt ctatgaaaac catcgtgttc gcgatcttgc tggtgatggt agtttccggg
     7381 tgttcgtc
//
LOCUS       DATLCH010000149         3325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1918411, whole genome shotgun sequence.
ACCESSION   DATLCH010000149 DATLCH010000000
VERSION     DATLCH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918411"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..927)
                     /locus_tag="VMZ02_04385"
     CDS             complement(148..927)
                     /locus_tag="VMZ02_04385"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71199.1"
                     /translation="MDGFADKIVLVTGGNSGIGAAIAGEFAKRRCRVVITARNATKLR
                     TAADKMAQDGAQIRSLVCDITRREQVRRLRDSVQELWGDVQILINNAGIARAVSFADM
                     SDEAWDQTLDTNLTGAYNCCKAFLPPMAEANWGRIINIASTAAKVGYSHVSAYTASKH
                     GLLGLTRSLALEAARTGITVNAICPGYVDDERTHDNARLMAEKTGKSVQDILRLFAAS
                     APQNRLIAVEEVAELACLIASSAMSGMTGQAINVDGGAVMI"
     gene            1046..2107
                     /locus_tag="VMZ02_04390"
     CDS             1046..2107
                     /locus_tag="VMZ02_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUR71200.1"
                     /translation="MPYRMIWLLLLLLFTPAALRAETKLGNSSLVNWREAQPSTKENE
                     LDRLNRAFVHLASESRPAIVQIRVNGPENSPNQGSRGSGFFIDPEGYILTAHHVIDKA
                     KDIEVRTAEGQRLAARLVVADSQLDLALLKVQSEPQATVLSLGDSERIQVGDLAIVFS
                     YPFGRESSMSMGIISRAGRTYPESAAYDFIQTDAGAYPGVSGGPLLNSQGHVVGMITM
                     ASERGNLGFATPINVIKKILPRLVKGEKFAWGWLGVQMADISLTQATAMGIYPAKGVV
                     VSSVLPGQPAARVGVQKLDVILAVDDSPVDSPRDVVRMVGGLEAGRMVQLSIMRKGKA
                     IRLTVPLGVKPETIKPREG"
     gene            complement(2115..>3325)
                     /locus_tag="VMZ02_04395"
     CDS             complement(2115..>3325)
                     /locus_tag="VMZ02_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lon-insertion domain-containing protein"
                     /protein_id="HUR71201.1"
                     /translation="PPGLRPEPIPTDTKVIMIGDPGLYRVLATADPDFRETFKVKADF
                     NFEVDRTQENITAFACFISDYCKREGIRHFDADGVARVIEQCARQVEDQNKLSTRFSD
                     MVDLLIESNYWAGKENAELISGKHVERALVEKTFRLNLIEKRLQELIAEGTVLVDVTG
                     AAVGQVNGLAVYQMGDFSFGKPSRITAKTFMGRGGLVNIERESKMSGRSHDKGVLILG
                     GYLGGKYAQQSPLSLSSTICFEQSYDGVDGDSASSSELYAILSSLSEAPIKQGIAVTG
                     SVNQNGEVQAIGGVNFKIEGHFDICRIKGLTGEQGVMMPQANVRNLMLRPDVVEAVKA
                     GQFHIYSVRSIDEGIEVLTGVDAGALGDDGKYPNDTINDRVQRKLQQFSDLQKKVTAG
                     EQKTAEPQNG"
BASE COUNT          728 a          970 c          875 g          752 t
ORIGIN      
        1 ccgttccaac tccgtatagg tttcgaaggt cagagcgttc aatcgctcag gatcgttcaa
       61 acgaatcgtc gcgatattgt cggcgacgtc aaacacgagc gaattgtaaa ccatgatcca
      121 ttctcgatta gagactatca attcttatca tatcatcacc gcgccgccgt cgacattgat
      181 cgcttgtccc gtcatgccgc tcattgccga tgacgcaatc aaacacgcca gttcggcgac
      241 ttcttcgacg gcgatcaaac gattctgcgg cgcgctagca gcgaaaagcc tgagaatatc
      301 ttgaacactc ttaccggtct tctcggccat gaggcgcgca ttgtcatgcg tgcgctcatc
      361 gtcgacataa cccggacaga ttgcattcac cgtgattccg gtgcgcgcgg cttccagagc
      421 caacgatcgc gttaacccga gaagcccatg cttagatgca gtgtaggcgc tcacatggct
      481 gtaaccgacc ttggccgcgg tagatgcgat gttgatgatg cggccccagt tcgcttccgc
      541 catcggcggt agaaatgctt tgcagcaatt gtaagcgccg gtgagattgg tatccagggt
      601 ttgatcccaa gcttcatccg acatgtcagc aaaacttaca gcccgggcga tgccggcgtt
      661 gttgatcaag atctgcacgt cgccccaaag ctcttgaacc gaatcccgca gccgccgcac
      721 ttgttcccga cgcgtaatgt cacagacgag ggaacgaatc tgtgctccgt cttgagccat
      781 cttgtccgcg gctgtccgga gtttagtggc attccgcgcc gtgatcacaa cgcgacaacg
      841 ccgttttgca aactcaccgg cgattgccgc accgatgcca ctattgccac cggtgactag
      901 aacaattttg tcagcgaacc catccacgat cgaagaatag catatgccct ggcgcgctgc
      961 gcaattcaat ccgcggctac gcttgtgatc gtccaaggtt ttcgataaat ggacgtccag
     1021 tcatgatccc agccgaggtg caatcgtgcc ataccgtatg atttggttat tattgcttct
     1081 gctttttact ccagccgcgt tgcgcgctga aaccaagctc ggcaactcat cgttggtcaa
     1141 ctggcgcgaa gctcaaccat ccaccaagga aaacgaactc gaccggctca atcgcgcctt
     1201 tgttcatctg gcgagcgaat cccgcccggc gattgttcag attcgtgtca acggaccaga
     1261 gaatagccca aatcaaggta gccgtggctc cggcttcttc atcgatcccg aaggttacat
     1321 cttgaccgct caccatgtca tcgacaaggc taaagacatc gaagtccgca ccgcggaagg
     1381 tcaacgcctg gcggcgcgat tggtagtcgc cgacagtcag ctcgacctag cgctcttgaa
     1441 ggtgcaaagc gaaccccaag cgacggtgct ttcgctcggt gattccgaac gcattcaagt
     1501 cggcgacttg gcgatagtgt tcagctaccc attcggacgt gagagttcga tgagcatggg
     1561 catcatcagt cgcgccggca gaacctatcc cgaatcagcg gcctacgatt tcattcagac
     1621 cgacgctggc gcgtatcccg gcgtcagcgg cggcccgcta ttaaacagcc aaggccacgt
     1681 cgtcggcatg atcaccatgg cttccgagcg cggtaatctc ggcttcgcca cgccgatcaa
     1741 cgtaataaaa aaaatcctgc cgcgcttggt taaaggcgag aagtttgcct ggggatggct
     1801 gggtgtccag atggcagata tttctctgac gcaagcgacg gccatgggga tctatccggc
     1861 caaaggcgta gtggtgagtt cggtcttacc ggggcaacca gcagcacgcg ttggcgtgca
     1921 gaaattagac gtcatactgg cagtcgacga cagcccggtc gacagcccgc gcgatgtggt
     1981 gcgtatggtg ggcggactgg aagccggacg catggttcag ctgtcgatca tgcgcaaagg
     2041 caaagcgatt cgtttgacgg tgcccctggg tgtcaagccc gaaaccatca aaccgcgaga
     2101 aggataaccc gccttcaccc gttttgtggc tctgcggttt tttgctctcc ggccgtcact
     2161 ttcttctgta agtcggagaa ttgctgaagc ttcctttgca cgcggtcgtt gatcgtatca
     2221 tttggatact tgccatcgtc gcccagcgcg ccggcatcga caccggtaag cacttcgatg
     2281 ccttcgtcaa tggaacgtac ggaataaata tgaaattgac cggccttgac cgcctcgacg
     2341 acatcgggac gcaacatcaa gttgcgcaca ttggcctgcg gcatcatcac tccttgttcg
     2401 ccggtcagcc ctttgatgcg gcagatatcg aagtgacctt cgattttaaa atttactccg
     2461 ccgatcgctt gaacctcgcc attttgattc accgaacctg tgactgcgat gccctgttta
     2521 atcggcgctt cggaaaggct cgaaagaatc gcataaagtt cgctcgatga agcactgtcc
     2581 ccgtcgacgc cgtcgtagga ctgctcgaaa cagatcgtgc tggagagcga tagcgggctt
     2641 tgctgcgcat acttgccgcc cagatatccg ccgagaatca gcacaccctt gtcatggctc
     2701 ctgccgctca tcttcgactc gcgttcgatg ttgaccagac caccgcgccc catgaaagtt
     2761 ttggcggtaa tgcgcgacgg tttgccaaaa ctaaaatcac ccatttgata aaccgccaag
     2821 ccgttcactt gacccaccgc agcgccggtg acatccacca gcaccgtacc ttcggcgatc
     2881 aactcctgga gccgtttttc gattaggttg aggcgaaagg ttttttccac caacgctcgc
     2941 tcgacatgct tgccggagat caactcggcg ttttcctttc cagcccagta attcgactcg
     3001 atcaacagat ccaccatatc gctaaagcgc gtcgatagtt tattctggtc ctcgacctgg
     3061 cgggcgcact gctctatgac tcgagcaacg ccgtcggcat cgaaatggcg gatcccctcg
     3121 cgcttgcaat aatcacttat aaagcaggcg aacgcggtaa tgttttcctg ggtgcggtcc
     3181 acctcgaaat tgaaatcggc tttgaccttg aaggtctcac ggaagtccgg atcggcagtt
     3241 gcaaggacgc ggtacagacc tgggtcgccg atcattatca ctttggtgtc cgtgggaatg
     3301 ggttccgggc gcagacctgg gggcg
//
LOCUS       DATLCH010000150         4723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3207912, whole genome shotgun sequence.
ACCESSION   DATLCH010000150 DATLCH010000000
VERSION     DATLCH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3207912"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1481)
                     /locus_tag="VMZ02_04400"
     CDS             complement(285..1481)
                     /locus_tag="VMZ02_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HUR71202.1"
                     /translation="MPVGTPFHPRTAALCASHNWRTWSGYFVASSYEVMHDYEYHAIR
                     NTAALIDVSPLYKYDVRGKDALKLVNRVITRDAAKCAVGQALYSCLCEDDGAVIQDGT
                     VFRLSDDYFRFHLADPSLRWLNLNARGMEVSIHEVSEQVAALALQGPHALKILQRVVD
                     VELNKLRFFRLACGKIHDVPVIISRTGYTGDLGYELWFGAEHALKIWDLLIEQGSDFG
                     IKPTGILALDVARLEAGFILLEVDYTGAEKAVIESQRYSPFEIGLGWTVDLGKDHFVG
                     AKALRQCDRQGPARQILGLEINLIDYEYLYQQAGLAPQYPLTAWRCSVPLHKDQRQVG
                     YATTGAWSPALKKYIALATLEKPCASVGKEVEIEVTVDHRQKNVSARVVKLPFFDPAR
                     KRAVLG"
     gene            1594..3174
                     /locus_tag="VMZ02_04405"
     CDS             1594..3174
                     /locus_tag="VMZ02_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUR71203.1"
                     /translation="MQNYDAIIIGGGHNGLTSAAYLAKAGKKVLVLERRHVLGGAAVT
                     EEIYPGFKYSVCSYVVSLLRPEVIRDLELPKYDLEIIPLDSTFTPMPNGDHLFRCGDH
                     SQTRREIARHSVTDAEAYEQYGQLMAQMALAVKPMLGMIPPEPTTWNPKDLFGLAKVG
                     SHFRDLGDKMVYELAKLMTMSSADFLDEWFETDVLKATMSASGIIGTFMGPRSPGTAY
                     VLLHHYMGELDGAFRSWGFARGGTGAISNAIADAARHFGAEIRTQVTVDQIMIKNGRA
                     TGVILATGEEIQAKTVVSSVDPKRTFLKMIDSQHLDAPFLQQVGNFKIRGSSAKVNLS
                     LDALPDFTSMPGNGPHLSGAISISPSLDYIERAYDDAKYGDFSRRPYMDIILPSMLDP
                     SMAPPGKHVMSIFVQYAPYHLASGTWPEKREALGDAVIETLSEYAPNLKKIILHRQVV
                     TPWDLEQEFGLTEGNIFQGELTLDQLFFLRPVPGWAKYRTPIKNLYMCGACTHPGGGV
                     MAASGRLAALEILKSSWM"
     gene            3201..3608
                     /locus_tag="VMZ02_04410"
     CDS             3201..3608
                     /locus_tag="VMZ02_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HUR71204.1"
                     /translation="MADIPITVFYDGHCGLCHGLVRFLIARDPNGGKFDFAPLQGEYC
                     ARVIPQVQRLPDSVVVRTDEGQLLVKSAAVLYVLRRIGRAWRIIGVALKILPTSILDF
                     GYDCVARIRRRLFRAPADTCPLVPATLQNRFHR"
     gene            3671..>4723
                     /locus_tag="VMZ02_04415"
     CDS             3671..>4723
                     /locus_tag="VMZ02_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUR71205.1"
                     /translation="MTHYDAIVIGAGHNGLVTAAYLAKAGKKVLVLERRATIGGIAVS
                     EEIFPGFRFSTCAHVASSFAGEISADLDLKKHGLEILPLDPLLFAPSQSGMPLVIGHD
                     PIKAAAEIARHSKNDASKYAEFCARLKTLATFLRSLYGLTLPDKASPDAFNPLELLKI
                     GLKFHGLGDKEGYEFLRILPMSMADLLHEWFEHDLLKASLAGSSMLASFVGPRQQGTA
                     FNLLHHQIGESNGALRTAGFVRGGIGHLTAALAGAARQHGAEIRADAEVVKIVTKQSA
                     ATAVALANGEEITANAVVSSADVKRTLLGLVEPTYLDPHFLLQVQNIRARGTVAKINL
                     ALDSLPRFKQQESPEQL"
BASE COUNT         1078 a         1327 c         1278 g         1040 t
ORIGIN      
        1 gctagaaatt cagagtctct cttcaggcca cgagatgatc aatagcttac ctcgtgaata
       61 gaggatggaa ttgtgaggaa gacctccaca cctgtttctc tcagtggttg tgccgctgaa
      121 gccattcgga gaaggagggg aatggatacc gctttcgccg ctatgacgga accttcgtgt
      181 caattcgtcg tctgctcatc cctggacatt tattttccaa ggaggcactt cacggtcatt
      241 tcgaccgagc aggagaaatc tttcttaatt tccaaaaagc cgattcaacc aagaactgcg
      301 cgtttgcgcg ccggatcgaa gaacggcagc ttgacgaccc tcgcgctgac gtttttttgg
      361 cggtgatcga ccgtgacctc aatttcgacc tctttcccca ccgatgcgca gggtttctcg
      421 agagtcgcga gagcgatata tttttttagc gcaggggacc aggcgccggt ggtggcataa
      481 cctacctgcc gttgatcctt gtgcaatggg acactgcagc gccaagcagt caaaggatac
      541 tgcggtgcca acccggcctg ctgatagaga tattcgtaat cgataagatt gatctccaag
      601 cccaggatct gccgcgccgg accttgtcga tcacattgac gtaacgcttt agcgccgacg
      661 aaatgatcct tacccaaatc aaccgtccag ccaagtccta tctcgaacgg agaatagcgc
      721 tgcgattcga tcacggcttt ttcggcgcca gtgtagtcga cttctaataa aataaatcct
      781 gcttccagcc gcgccacatc gagggcgagt atgcccgtgg gtttgatgcc gaaatcgctt
      841 ccctgctcga tcaagagatc ccagatcttg agcgcatgct cggcgccgaa ccacagctcg
      901 taacccagat caccggtgta gcccgtacgc gaaataatca cggggacatc gtgtattttg
      961 ccgcaggcaa gacgaaagaa tctgagctta tttaattcaa cgtcgacaac ccgttgaaga
     1021 attttcaaag catgggggcc ctgcaatgcc aaagcggcca cttgctcgga aacttcgtga
     1081 attgatactt ccatgccgcg ggcgttgaga ttaagccagc gcaagctcgg atcggcaaga
     1141 tggaagcgaa agtagtcatc gctcaggcga aacaccgtgc cgtcttggat caccgcgccg
     1201 tcgtcctcgc ataagcagct gtagagcgct tgaccgacgg cgcatttcgc cgcgtcgcgg
     1261 gtgatcaccc gattgaccag cttgagtgcg tctttgccgc gcacgtcgta tttgtaaagc
     1321 ggcgaaacgt cgatgagcgc agcggtattg cggatcgcgt gatattcgta gtcgtgcatc
     1381 acttcataag agctggcgac gaaatagcca gaccaagtgc gccagttgtg gctggcgcat
     1441 aacgccgcag tgcgcgggtg gaagggagtt ccgacgggca taattatttt ttagaatctg
     1501 ctgcggagcg cgtttggatt ttcggaatcg tactatctcg ttgacttcgc aaacgaaatt
     1561 accaaatatg ctgctttaag caaatctccc tctatgcaaa actacgatgc gatcattatc
     1621 ggcggaggcc acaacggcct gaccagcgct gcctatcttg ctaaagccgg taaaaaagtt
     1681 ctggtgctgg aacgacgcca tgttctcggc ggcgcggcag tcaccgaaga gatttatccg
     1741 ggatttaaat actcggtgtg ctcctatgtc gtgagcctgc tccggcctga agtgatccgc
     1801 gatttagaat tgccgaaata cgatctggag ataattccgc tcgatagcac gtttacaccg
     1861 atgcccaacg gcgaccatct gttccgctgc ggtgatcact cgcagacgcg ccgggagatc
     1921 gcacgccatt cggtgaccga tgccgaggct tatgaacagt acggccaact gatggcgcag
     1981 atggccttgg cggttaagcc tatgctcggc atgatcccgc cggaaccgac aacttggaat
     2041 ccaaaagatc tattcggttt ggccaaggtc ggcagtcatt ttcgcgatct tggcgacaag
     2101 atggtctatg agcttgccaa actcatgacc atgagctcgg cggatttcct cgacgagtgg
     2161 tttgaaaccg acgtgctcaa agcgactatg tcggccagcg gtatcatcgg cactttcatg
     2221 ggaccgcgct cgccgggaac cgcttacgtg ctcttgcatc actatatggg ggaactcgat
     2281 ggtgcatttc ggtcctgggg ttttgcccgg ggcggcaccg gtgcgatcag caacgccatc
     2341 gccgacgcgg cgcgtcattt tggcgcagag atcagaaccc aggtgaccgt tgatcaaatc
     2401 atgattaaaa atgggcgcgc caccggcgtc atcctagcaa cgggcgaaga gatccaggcc
     2461 aaaaccgtag tctccagcgt cgatcccaaa cgaacttttc tgaaaatgat cgacagccag
     2521 catttggacg cgccattctt gcagcaggtg ggaaatttta aaatccgcgg ctcgtctgcg
     2581 aaggtcaacc tttctctcga tgccctgcct gacttcacca gtatgccggg caatggcccc
     2641 catttatcag gcgcgatctc gatcagccca agcttggact acatcgagcg cgcctacgac
     2701 gatgccaagt acggcgattt ttcgcgccgc ccctacatgg atatcattct tccatccatg
     2761 ctcgacccat cgatggcgcc gccagggaaa catgtcatgt cgattttcgt ccagtatgcg
     2821 ccctatcatc tggctagcgg tacgtggccg gagaagcgcg aggccttagg tgatgctgtt
     2881 atcgaaacgc tgagcgagta cgcgcccaat ctgaaaaaaa tcatcttgca tcggcaggtg
     2941 gtcacgccct gggatttgga acaggagttc ggtctcaccg agggcaacat atttcaaggt
     3001 gaactgacac tggatcagct tttctttctg agaccagtgc cgggttgggc aaaataccgc
     3061 acgccgatta aaaatctcta catgtgcggc gcctgcaccc atcccggcgg cggcgtgatg
     3121 gcggcatccg gcagactggc ggctttggaa atcttaaaaa gctcgtggat gtagcgctat
     3181 tcgaaagcac cggcaaatcg atggccgaca tccccattac ggtcttttat gacggccatt
     3241 gcggtctctg tcacggcttg gtgcgcttcc taatcgccag agatcctaat ggcggaaagt
     3301 ttgactttgc accactgcag ggcgagtact gcgccagagt aattcctcaa gtgcagcgct
     3361 taccagacag cgtggtggtc cgaaccgatg aaggacagtt gctggtcaag tcagcagccg
     3421 tgctctacgt tctcaggcga atcggcagag cctggcgcat tattggagtg gcattgaaga
     3481 ttttaccaac ttcgattctc gatttcggct atgactgcgt cgcgcggatt cgccgacggc
     3541 tattccgcgc acctgcagat acttgtccac tggtcccggc tacgttacag aatcgctttc
     3601 accgctagcc acagcgcaaa aggttgagaa actttgacat ggtaaaaaaa ccaaatggaa
     3661 tgactgagcg atgactcact acgacgccat cgtcattggc gccggccata acggtctagt
     3721 caccgcggcc tatctcgcca aggccggcaa aaaagtcctc gtcctcgaac ggcgcgctac
     3781 tatcggcggc atcgccgtca gcgaggaaat attcccgggt tttcgttttt cgacctgcgc
     3841 gcatgtcgcc agcagctttg ccggcgaaat cagcgccgat ctcgatctga aaaagcacgg
     3901 cctagagatt ttgccgctcg atccattact gttcgcaccg agccagagcg gcatgccact
     3961 ggtgattggg cacgatccca taaaagcggc cgcagaaatc gcccgtcact cgaaaaacga
     4021 cgcgagcaag tacgccgagt tctgcgccag actcaaaact ctcgcaactt tcctgcgcag
     4081 cctttacggc ctgactctcc cggacaaagc gagtcccgat gcattcaacc ccttggagct
     4141 tttgaagatc ggcttaaaat tccacggtct cggtgacaag gaaggctatg agttcctgcg
     4201 cattttgcca atgagcatgg ccgatctgct gcacgagtgg ttcgagcatg atctattaaa
     4261 ggcgagtctt gcgggaagct ccatgctggc ctcgtttgtc ggaccgcgtc agcaaggcac
     4321 ggcgtttaat ttgctgcacc accagatcgg cgaatccaac ggcgcgctgc gcaccgccgg
     4381 cttcgtgcgc ggcggtatcg gccatctcac cgccgctctt gccggcgccg cacggcaaca
     4441 tggcgcggag atccgcgccg acgccgaggt cgtgaaaatc gtaaccaaac aaagcgcagc
     4501 aacagcggta gcgctggcga acggtgagga gatcaccgcg aacgccgtgg tttccagcgc
     4561 cgacgtgaag cggacgcttc tcggtcttgt cgagcccacc tacctggacc cgcatttttt
     4621 gctgcaagtg caaaatattc gcgcccgcgg cacggtggca aaaatcaacc tcgcgttgga
     4681 cagcttgccg agattcaaac agcaggaatc cccagaacag ctc
//
LOCUS       DATLCH010000151         5663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4900387, whole genome shotgun sequence.
ACCESSION   DATLCH010000151 DATLCH010000000
VERSION     DATLCH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4900387"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..927
                     /locus_tag="VMZ02_04420"
     CDS             247..927
                     /locus_tag="VMZ02_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HUR71206.1"
                     /translation="MNAQPLLRLIDQISYWTGKAFAWLILALTLVVSVEVFKRYILNA
                     PTSWIFDLNNMLYGTLFMMCGAYTLALAGHVRADFVYIYLKPRAQASLDLILYFLFFL
                     PGILGLIYAGYGYAADSWRIAEHSNVTAEGPPIYYFKTVIPIAGAFVMLQGLAEIVRC
                     ILCIKTGAWPARLDDVEEIDVIESQLSHSEFIDEESRLATMRSAHAIDDAARHRSSMD
                     MDEKEKKP"
     gene            924..3002
                     /locus_tag="VMZ02_04425"
     CDS             924..3002
                     /locus_tag="VMZ02_04425"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HUR71207.1"
                     /translation="MSDPLLGLTMLGLIVVAIMMGFPTAFTLMGLGMIFGYIAFWDPA
                     HHWWQNRAFDLMVQRTYGVMTNDTLLSVPLFVFMGYIMERAALVDRMFHSVQLAFRRV
                     PASLAVTTLLVCAFWGIASGIVGAVVVLMGVIAMRPMLNAGYDVRLASGAITAGGTLG
                     ILIPPSVMLIVYAAVAGQSIVKLYAAAMIPGFFLTFLYLLYILGWAIINPKIAPQLPP
                     DQYRVKIPEWLKRLERSPSKRVSSGIIAALFQPGKLKGATRPDGKPVSYGFIVSNVIA
                     LMVPLALTIGTYAGTWWYVVIHNAPATTTSPEANPQLPAPPALTDKPQELGSTEEKPQ
                     ELGNAEEKPAELGSAESTVESTETVRGADAPPQEMTAVTEQPSTAPAGGRVVPRQFYT
                     WFWTLAGLSTLLLLYYYWRMDGEQFEILKELSVAVVPLGVLTIVVLVVILLGICTATE
                     SAAIGALGALYLAVLVKFPHSCGWWSLLGAIIGVALGMQRGDIATLIVSGSLGATFVG
                     TGIPLLIALPKSPELRRNLKEATFLTAKTTAMVCWLFIGSALFSAVFALHGGQGLIER
                     WVLSLNLSPLGFQLVAQAIIFLLGWPLEWTEIIVIFCPIFIPLLSHFHVDPILFGTMV
                     AVNLQAAFLSPPVAMSAFYLKGVAPKHVTLNQIFAGMMPYMIIVCLCLVCMYIWPGMT
                     LWLPEFLYGN"
     gene            3111..4409
                     /locus_tag="VMZ02_04430"
     CDS             3111..4409
                     /locus_tag="VMZ02_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HUR71208.1"
                     /translation="MRLTRDGVISSEQLVEACLAQIRAVDENIQAWAFLDPEYALTQA
                     RALDQLRLTGQPIGPLHGIPVAVKDIFDTADMPTEYGSPIHVGRTPSRDATAVALLRA
                     AGAVIMGKAVTTEFAFLSPGKTRNPHNPAHTPGGSSSGSAAAVASNMVPLAIGSQTAG
                     STIRPAAYCGVIGFKPTHGLISRHRVSPLSRTLDHVGLFARTIDDIALLAEQLVAHDE
                     NDPDTQPRARIPFSTISTEEPPLAPMFAFMKTPMWQRADEETKEGFAEIVEQLGAQVE
                     EVELFPSAQEAWQWHQTIMAAEVAHNFEREWQNCRALLSEQLRAQIERGLEVRALDYQ
                     RALKQIAPMHDSFVELFEQRYDAILTPAAPSAAPLGLMSTGDPAFCTLWTLCGMPCIT
                     LPLLQSTNGLPIGIQLVGPRGGDARLLRTARWLAAKMVES"
     gene            complement(4417..4782)
                     /locus_tag="VMZ02_04435"
     CDS             complement(4417..4782)
                     /locus_tag="VMZ02_04435"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71209.1"
                     /translation="MKVLRMRSWNIHADNLSHMARFYQDALGAEITAEQTIAGVKVVR
                     LRTGDTGLGLFDAAEKRAPGVPHHTFDIEGPGNGQDLIDELEAKGIKTDGMRLHGQGP
                     GYSVYIVDPSGNRLELSKD"
     gene            complement(4828..>5663)
                     /gene="lipA"
                     /locus_tag="VMZ02_04440"
     CDS             complement(4828..>5663)
                     /gene="lipA"
                     /locus_tag="VMZ02_04440"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HUR71210.1"
                     /translation="PDWIKVAAPGNGNYLRLKKLMREKNLHTVCEEARCPNIGECWGN
                     KTATFLILGDTCTRGCRFCAIDKGKPLALDPEEPRNVGLAVKDLSLDHIVVTSVNRDD
                     LSDGGANHFAKTVFWIKNLNPGIRVELLIPDFEGNLDALKTVISSGIEILNHNIETVP
                     RLYGKVRPGHTYQCSVNILKTAKDMQPGVLTKSGMMLGVGETKDEVMATLHDLKANNV
                     DIVTLGQYLQPAPKLLKVERYLHPEEFKEFKIEADKLGFRHVESGPLVRSSYHAWSHV
                     D"
BASE COUNT         1111 a         1584 c         1615 g         1353 t
ORIGIN      
        1 gtcttcgatc aagtggaggc aaaaaaggcg gctgagaatc ccttattcaa agaggtctcc
       61 gagtcgcagc gcgccttcgc ggcacgcgcc gtgaagtggg acctcgacac caacgttgcc
      121 cgccgcatgg cttacaacca ctattttgcc aagcctgcgg ccaagaaggg ttaatcctcg
      181 gaccctttgc cgctattcgc cagtcatccg gcagcaccgg atgactggcc tttttttaga
      241 aaaattatga acgctcaacc actgctccgt ctgatcgacc aaatcagcta ctggacgggc
      301 aaggcgtttg cctggctgat cctggcattg acgctggtgg tatcggtcga ggtgttcaaa
      361 cgctacattt tgaacgcgcc gacttcgtgg atcttcgatc tgaacaatat gctttacggc
      421 acactattca tgatgtgcgg cgcctacact ttggcgctcg cgggtcatgt gcgcgccgac
      481 ttcgtgtaca tttatctaaa gccgcgcgcc caggcgtcgc ttgatctgat tttgtatttt
      541 ctcttttttt tgccgggcat actggggctc atctatgcag gctacggcta cgccgccgat
      601 tcttggcgca tcgccgaaca ttcgaatgta acggcggaag gtccgcccat ctattacttc
      661 aagacggtta ttccgattgc cggggcgttt gtcatgctcc agggcttggc ggaaattgtc
      721 cgctgtattc tatgcatcaa gaccggcgcc tggccggcac gtctggacga cgtcgaagag
      781 atcgatgtca tcgaaagcca actttcgcac agcgaattca tcgatgagga atcgcggctc
      841 gcaaccatga gatcggcgca cgccatcgac gacgcggcac gtcatcggag ctcaatggac
      901 atggacgaga aagaaaaaaa accatgagcg atccgctact cggcttgacg atgctgggtc
      961 tcatcgtggt cgcgatcatg atggggttcc cgaccgcatt cacgctcatg ggcttaggca
     1021 tgatcttcgg ctacatcgcg ttctgggatc cggcccatca ctggtggcag aaccgggcct
     1081 tcgatctgat ggttcagcga acttacggcg tcatgaccaa cgacacgctg ttatcggtgc
     1141 ctttgtttgt ttttatgggt tacatcatgg aacgcgccgc gctggtcgac cgcatgtttc
     1201 acagcgtgca gctggcgttc cgccgcgtgc cggcgtccct ggcggttacc actttactcg
     1261 tctgtgcttt ctggggcatc gcttccggca ttgtcggcgc tgtcgtcgtg ctcatgggtg
     1321 tgattgcgat gcggccgatg ctcaacgctg gctacgacgt ccggttggcc tcaggagcaa
     1381 ttaccgccgg cgggactctc ggtattctca ttccaccgtc ggtcatgctg atcgtctatg
     1441 ccgccgtcgc cggccaatcg attgttaaac tctatgcggc ggcgatgatt cccggttttt
     1501 tcctgacatt tctctatctt ctctacattc ttggctgggc gatcatcaat ccgaaaatcg
     1561 cgccacagct gccgccggat caatatcgcg tcaaaattcc cgagtggctg aagcgtctgg
     1621 aacgaagccc ctcaaagcgc gtgtctagcg gtattatcgc ggcgcttttt caaccaggca
     1681 aactaaaagg cgcaactcgg ccggatggta aaccggtcag ttacggtttc attgtcagta
     1741 atgtgatcgc gctgatggtg ccattggcgc tcacgatcgg aacctacgcc ggcacctggt
     1801 ggtatgtcgt cattcacaat gcgccggcga cgactacctc gccggaggct aacccgcaat
     1861 tgccagcgcc acccgcacta acggacaaac ctcaggagct tggcagcact gaagagaagc
     1921 cgcaggagtt ggggaatgcg gaagaaaaac cggcggagct tggttcagcg gagtctactg
     1981 tcgagtcgac agagaccgtg cgcggcgccg acgcgccgcc acaagagatg actgcagtga
     2041 ccgaacagcc gagcaccgcg cccgcggggg gacgagtcgt accgaggcaa ttctatactt
     2101 ggttttggac tctggcgggg ctgtcgacgc tgctactgct ttactactac tggcgcatgg
     2161 acggtgagca gttcgagatt ctcaaagaac tgtcggtcgc cgtggtgccg ctcggtgtgc
     2221 tgaccatcgt tgtattggtg gtgatactcc tgggaatttg tacggcgacg gagtcagccg
     2281 ccatcggcgc cttgggagcg ctctatctcg cagtgttggt gaagttcccg cacagttgcg
     2341 gctggtggag cctgctgggc gctataatcg gtgtggcgct gggcatgcaa cgcggcgata
     2401 tcgcgacatt gatcgtgtcc ggatcgcttg gcgcgacctt tgtcggcact gggattccgc
     2461 tcttgattgc cctgcccaag tcacccgagc tccggcgcaa tctcaaggaa gccacctttc
     2521 tcactgccaa aaccaccgcg atggtatgct ggttgttcat cggctcagcg ctattttccg
     2581 cggtctttgc tttgcacggc ggtcaagggc tgatcgaacg ctgggtgttg agtttgaatc
     2641 tatcgccgct gggttttcag ctagtcgccc aggcgattat ttttttgctg ggctggccac
     2701 tcgaatggac cgagatcatc gtgatcttct gtccgatctt tattccgctg ttgagccatt
     2761 tccacgtcga tccgattttg ttcggcacga tggtggcggt caatctacaa gcggcattct
     2821 tgtcaccgcc agtggcgatg tcggcgtttt atctcaaagg cgtggcgccc aagcatgtta
     2881 cgctgaacca aatctttgcc ggcatgatgc catacatgat catcgtctgc ttatgtctgg
     2941 tgtgcatgta tatctggccg ggaatgacgt tgtggctgcc ggaatttctg tacgggaact
     3001 aaaacattca acgcattgaa gggttcaaat aattcaaaga tgtcgaaaaa tttgcgctat
     3061 atcgttccat gacattaacc aatcttcatc tgctttctgc ctccgaaggg atgcggctaa
     3121 ctcgcgatgg ggtgatttct tcggagcaac ttgtcgaagc ctgcttggcg cagatacgcg
     3181 ctgtcgatga aaacattcaa gcctgggcat ttctcgatcc cgaatatgcg ctgacgcaag
     3241 cccgggcgtt ggatcagttg cgcttgactg gtcagccaat aggaccgctc cacggtattc
     3301 ccgtcgcggt caaagatatt ttcgacaccg cggacatgcc gacggagtat ggttcaccga
     3361 ttcacgtcgg acgcacaccg tcgcgcgatg ccacggcggt ggctttgctg cgtgctgccg
     3421 gagcagtgat catgggcaaa gcggtgacta ccgagtttgc tttcctcagc ccaggtaaaa
     3481 ctcgcaatcc acacaatccc gcgcatacgc ccggcggctc ctcaagcggc tcggcggcgg
     3541 cggtggcgtc gaacatggta ccgctcgcga tcggcagcca aacggctgga tcgacgattc
     3601 gcccggcagc ctattgcggt gtgatcggtt tcaagccgac ccatgggctg atttcgcgcc
     3661 atcgggtatc gccgctttct cgcaccctcg atcatgtcgg tctgtttgcc cggaccatcg
     3721 acgatatcgc attgctcgct gaacagttgg tcgcgcacga tgaaaacgat cctgacactc
     3781 agccgcgcgc tcggattcca ttttcaacca tttcaactga agagccgccg ctcgcgccga
     3841 tgttcgcttt catgaagacg cccatgtggc agcgcgccga tgaggaaacc aaagaagggt
     3901 tcgctgaaat cgtcgagcag cttggtgctc aagtggaaga agtggagctg tttccgtcgg
     3961 ctcaggaggc ttggcaatgg caccagacga tcatggccgc cgaagtggca cataacttcg
     4021 aacgtgaatg gcaaaactgc cgtgcgctgc tatccgaaca actacgcgct cagatcgaac
     4081 gcggactcga ggtgagggcg ttggattatc aacgagcgct caaacagatc gcaccgatgc
     4141 acgacagttt cgtcgagctt ttcgagcaac gctacgatgc aatcctgaca ccggcggcgc
     4201 cgagtgccgc gcccctaggg ctaatgtcga ccggcgaccc agcattttgc actctgtgga
     4261 cgttatgcgg aatgccatgc atcactttgc cgctgctgca gagcactaac ggtctgccca
     4321 tcggcataca actcgtcggt ccgcgcggcg gcgacgcgag actcttacgc acggcgcgct
     4381 ggctggcggc gaagatggtg gagagctaga agtcgtttag tctttggaca attcgaggcg
     4441 attgccgctc gggtcgacga tgtaaaccga gtatcccggg ccttggccgt gcagtcgcat
     4501 gccatcggtt ttgatgcctt tggcttcgag ctcgtcgatc aaatcttgcc cattgcctgg
     4561 tccttcgata tcgaaagtgt ggtgcggtac gcccggcgct cgtttctccg cggcatcgaa
     4621 cagacctaat ccagtgtcgc cggtgcgcag gcggacaact ttgacgccgg cgatggtttg
     4681 ttcggcggtg atctcggcgc ccagcgcgtc ttgatagaaa cgcgccatgt gactgagatt
     4741 atcggcatgt atgttccacg agcgcatgcg taacactttc attgcatcct ccttcgggtg
     4801 gattgataag acccattcgt ttaccgccta atcgacatga ctccaggcgt ggtaggaact
     4861 gcgtactagc gggccggact caacatggcg gaagccgagc ttgtcggctt caatcttaaa
     4921 ttctttaaac tcctccgggt gcaggtagcg ttcgactttc aacagtttcg gcgcaggttg
     4981 caagtattgt ccgagcgtca cgatatcgac attgttcgct ttcaaatcgt gcagtgtcgc
     5041 catcacttcg tctttggttt cgccgacgcc gagcatcatg ccgctcttgg tgagaacccc
     5101 cggctgcatg tccttcgcgg tttttaatat gttaaccgag cattggtatg tgtgacccgg
     5161 ccggaccttg ccgtacaggc gcggcacggt ctcgatgttg tggttcaata tttcgattcc
     5221 cgagctgata acggttttaa gggcgtcaag gttgccctcg aagtcgggaa tcaacagctc
     5281 gactcggatg cccggattca aattcttgat ccagaaaacc gtcttggcga aatgattggc
     5341 gccgccgtcg gaaagatcgt cgcgattgac cgacgtgacc acgatgtggt ccaagctcaa
     5401 atctttgaca gcgaggccga cattgcgcgg ttcctccggg tcgagtgcca gcggcttgcc
     5461 tttgtcgatg gcacagaagc ggcagccgcg cgtgcaggtg tcgccgagaa tcagaaaggt
     5521 tgcggttttg ttgccccagc attcgccgat gtttggacag cgcgcttcct cgcaaacggt
     5581 gtggaggttt ttctcgcgca tcaatttttt taaccgcagg tagtttccat tccccggcgc
     5641 tgcgactttg atccaatcgg gat
//
LOCUS       DATLCH010000152         6857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9909141, whole genome shotgun sequence.
ACCESSION   DATLCH010000152 DATLCH010000000
VERSION     DATLCH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9909141"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VMZ02_04445"
     CDS             complement(<1..914)
                     /locus_tag="VMZ02_04445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUR71211.1"
                     /translation="MSIRAVLITLCLLAIAVLPNRTAADVIEQVIAVIDGEPYTLSNL
                     RTFAKARLAKSFPTGNLQEINAGDRDVLEQFVTDKLIEAEVREAGIRITDDDVDRYIE
                     QVKKNNRLSDEDFKTALTRDGMTLAGYKISVKTELEKAEILDRQVKRKVSITDEDVER
                     YYKLNSKNYRTNERARFRHILLTLSEKAPPDQVNAVSAKARELYSKIAAGEDFAAVAR
                     EYSEGAGRAEGGDIGWVNKGSLIGGLEEIAFEKLRPGQVSEPFRTSMGIHIVKLEARE
                     AGTVLPLTTVAPKIKQELLNKALEERFS"
     gene            complement(1072..4623)
                     /gene="mfd"
                     /locus_tag="VMZ02_04450"
     CDS             complement(1072..4623)
                     /gene="mfd"
                     /locus_tag="VMZ02_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HUR71212.1"
                     /translation="MTVSPLSERLAQFLRGNWSASKRIQGIQGGARAYLIALAAAEIQ
                     RPVLVIAPSANHAENLYDDLAFFLGEERGLAPLRRRLHLFPSWEVLPFENLSPHPENI
                     AGRLEGLYKLVEEPAPILIATPAALMQRVIPKEALKKSYLYIVAGQDLPRESLLDHLI
                     HWGFQNVPLVEERGDFSVRGGIVDLYPPGYGRPIRLEFDNDRLESIREFNPANQRTET
                     VQEELILLPMKEFALKRAGVDESIRRLEQRALELEVNRRERQALLESLREGIPVAGGE
                     FLLPFFCPGLVPVTSYLPDNTLVWLDGADRIEAESERFAQHAWERNRLAIEEHRLVAP
                     VEQLYLNEHEWRQALMPFSTVHGEALMLLTPAEKAQETTLNVESFLTTDLRQDPALHG
                     KDVSLAPLVERLKSWSAEKVIFVAPTKGDAMRLRELLGGYQIDAALIEDGLPAVLERQ
                     DIARGIVRGNLNQGFRLPEEHLIFITFDEIFGTRKRQPTTATKSYPSHFLSSLSELKQ
                     DDYVVHLDHGIGVYRGLKFLKVAAVEGEFLHLEYEAGDRLYLPVDRINMVQKYIGGDG
                     AQPSLDRLGGTAWEKVKAKAKKTVLAMAEELVQLYALREARGGTAFAPPDNMYKEFEA
                     AFEFEETPDQQRAIDDTLTSMQSKKPMDRLVCGDVGYGKTEVAMRAAFLAVEGGKQVA
                     VLAPTTILAQQHMQTFRHRFRNHPVRIEMVSRFLTSKEVAQVLSDTAKGQVDIVVGTH
                     RLLQKDVEFKDLGLVIIDEEHRFGVVHKERLKKMRALVDVLSLTATPIPRTLHMSLVG
                     IRDLSIIETPPVDRLAIQTYVTRYDDRVIRDAILREIERGGQVFFLHNRVETIDRLAL
                     KLSELIPEARMAVAHGQMRPKELEKVMLDFLENKTQVLVCSAIIESGLDFPNANTIII
                     NRADRFGLAQLYQLRGRVGRSHRHAFAYLLIPGEHAITPDAERRLRALQEIDGLGGGF
                     KLALHDLEIRGAGNLLGDQQSGQITAVGFELYTEMMQEAIQELKGEEVLPEIDPEIRL
                     GISAYFPDDYMPDANQRLYFYKRLASLKNSAELDELKAEIEDRFGPYTDVVENLFLVM
                     NLRRVLKEFLVQQISFSEGKIFLLFHPDSRVKVDKLLDLIHKHKNRFRLAPDGRLSFT
                     PKNHDWQPLAAEVVELLQTIREIPAPKVAEPAVGRVG"
     gene            complement(4620..4988)
                     /locus_tag="VMZ02_04455"
     CDS             complement(4620..4988)
                     /locus_tag="VMZ02_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HUR71213.1"
                     /translation="MDARLREGISLFNDQKFFECHEVLEGYYQDSELGTKPFLEGLIQ
                     LAAAFRMFVDFGEVKGSVRAIYQALIRLENYQPVYLQIRVKDFSTEVEAWAKAAEAAR
                     ATPTAANIPKIRLQRFSIFS"
     gene            complement(5094..6329)
                     /locus_tag="VMZ02_04460"
     CDS             complement(5094..6329)
                     /locus_tag="VMZ02_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71214.1"
                     /translation="MRFSPTPWLIAAFAFVVLGFSRGLHSSFGVFNVALLDSFGWSRG
                     ATAGIFSIVLGVDALLSPAAGYLLDRFGTRRIAIAGCLTLAVGLFLSSQVSVLWELYI
                     CFGLVLATGFTLTGMVPHIFLISEWFSSNRASAIGVVYAGSGVGIMLLAPLSAWLIYA
                     YGWQRAFEIYAAMVLVLLLPIVLLSYQPGPHGHSGAHGGQKKGEQNQWTAKLALQSLQ
                     FWLLFIARIGAASGTTVIVTHQVAHVVDVGFSKLLAASIFGLAGITSSFGRVIFGFIA
                     DHLTKQAAYSLNIAMTIVGVGALMILRDPSQAWLLYVYVIFFGIGFGSRAVIFSSLTA
                     DIFAGKGFGSILGYSTVAVGVGGALGSYFGGVFHDWTGSYTMSFTLSTLLLALSDVCI
                     WLAAVPAVAQYDQRLWPRH"
     gene            6542..>6857
                     /locus_tag="VMZ02_04465"
     CDS             6542..>6857
                     /locus_tag="VMZ02_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR71215.1"
                     /translation="MAGRQALLRGEPDNAVNYFNAIAQADPSYIVTLAPPRKSIWTYV
                     GRAHYNSGRYAQAKDAFDKALTHLKDDYIAQLYRGLTLLRGYTSPPPANAMTLQEISY
                     ALR"
BASE COUNT         1600 a         1979 c         1843 g         1435 t
ORIGIN      
        1 ttggaaaaac gctcttccaa cgctttgttg agcagctctt gcttaatctt cggcgccacc
       61 gtcgtgagcg gcagaacggt accggcttcg cgcgcctcga gtttgacgat gtgaatgccc
      121 atactcgtgc ggaagggttc gcttacttgg ccgggccgca gcttttcaaa agcaatttct
      181 tcgagacccc cgatcaacga acctttattc acccagccaa tatcgccgcc ctcggcgcgc
      241 ccggcgccct cggaatactc ccgggcgacg gcggcaaagt cctccccggc ggcaatcttg
      301 ctgtacaact ccctggcctt ggcagatacg gcattgactt gatcaggtgg cgccttctcc
      361 gacaacgtca atagaatatg acggaatcgc gctcgttcat tggtccgata gtttttcgag
      421 ttaagcttgt aatagcgctc gacatcttca tctgtgatgc tcactttgcg tttgacttga
      481 cggtcgagaa tttcggcctt ttcgagctcg gttttgaccg agattttata gcccgccaaa
      541 gtcattccat cgcgcgtcag cgccgtcttg aaatcttcgt ccgacagccg attgttcttc
      601 ttgacttgtt caatatagcg atcgacatca tcatccgtga tccgaatacc ggcctcgcgc
      661 acttccgctt cgatcagctt gtccgtgaca aactgttcca ggacatcccg atcgccggca
      721 ttgatctctt gcaaatttcc ggttggaaaa cttttcgcga ggcgagcttt tgcgaaggtt
      781 cgcagattcg atagggtata aggttcgccg tcaatcacag cgatcacctg ctcaatcaca
      841 tctgccgcag ttctgttcgg cagaacggcg atcgccaaca agcataaagt gattaatact
      901 gctcgaatgg acatgctcca agcctaaccc acggaatata taacagctaa catagtcgtt
      961 ttataattcc aaccggcgct tgcctaggaa taaacgtcgg ggactgtcaa atgtagctag
     1021 tgcgaaagcc caacagcgag ttttctcatc actccggtac gccagcactc atcaccccac
     1081 tcttcccacc gccggttcgg caaccttcgg cgcgggaatt tcccgaatgg tctgcaacaa
     1141 ctccaccacc tcggcggcca agggttgcca gtcgtgattc tttggcgtga aggagagacg
     1201 gccgtcaggt gccaagcgga aacgattttt gtgtttgtga atgaggtcga gcagcttgtc
     1261 caccttaacc cgggaatcgg ggtggaagag cagaaaaatc ttgccttccg agaagctgat
     1321 ctgctggacc agaaactctt ttaaaacccg ccgtagattc atcactagaa ataagttttc
     1381 gacgacgtcg gtgtaggggc cgaagcggtc ttcgatctca gccttgagct cgtcgagttc
     1441 cgcgctgttc ttcaaactgg cgaggcgctt ataaaaatac agccgctggt tggcgtcggg
     1501 catatagtcg tcgggaaagt acgccgaaat gcccagacgg atttccggat cgatctcggg
     1561 caaaacttct tcgcctttga gctcctggat cgcctcctgc atcatctccg tataaagctc
     1621 gaatccgacg gcggtgattt ggcccgactg ttgatcgccg agcaaattac cggcgccgcg
     1681 aatctcgaga tcatgcagcg ccagtttgaa accgccgccg agaccatcaa tttcctgcag
     1741 cgcgcgcaag cgccgctcgg catcgggcgt gattgcgtgc tcaccgggaa tcaaaagata
     1801 ggcaaatgca tggcggtgcg aacgccctac ccggccgcgc agttgataga gctgggccaa
     1861 gccgaagcgg tcggcgcggt tgatgatgat cgtgttggca ttaggaaagt cgagacccga
     1921 ttcgatgatc gccgaacaga ccagcacttg ggtcttgttc tccaaaaaat cgagcatgac
     1981 cttttccagc tctttgggcc gcatttggcc atgagccacc gccatcctgg cctcgggaat
     2041 caactccgat aacttgagcg ccaggcggtc gatggtctcg accctattgt gcaggaaaaa
     2101 cacctggccg ccgcgctcga tttcgcgcaa gatggcatcc cggatcaccc ggtcgtcata
     2161 gcgcgtgaca taggtttgaa tcgccagccg gtcgaccggt ggggtttcga taatgctcaa
     2221 atcgcgaatg ccgaccagcg acatatgcag ggtgcgcgga atcggcgtcg cggtcagact
     2281 caagacatcg accagcgcgc gcattttttt gagccgctct ttgtgcacca cgccgaaccg
     2341 gtgctcctcg tcaataatca ccaagcccaa gtctttgaac tcaacgtcct tctgcaacag
     2401 ccgatgggta ccgacgacga tatcgacctg gcctttcgcc gtgtcgctga ggacctgggc
     2461 gacttcttta ctggtcaaaa atctgctgac catttcgatg cgcaccggat gattgcggaa
     2521 gcgatggcga aaagtctgca tgtgctgctg cgccaaaatc gtcgtcggcg ctaggaccgc
     2581 gacctgcttg ccgccctcga cggcaagaaa tgccgcgcgc atcgccacct cggttttacc
     2641 gtagccgaca tcgccgcata ctaagcggtc cattggcttc ttgctttgca tgctcgtcag
     2701 ggtgtcgtcg atggcgcgct gctgatcggg cgtctcctcg aattcaaagg ccgcttcaaa
     2761 ctctttgtac atgttgtccg gcggcgcgaa cgccgtgccg ccgcgcgctt cgcgcaaggc
     2821 atagagctgg actagctctt cagccatggc aagcacagtc tttttcgcct tggctttaac
     2881 cttttcccag gcggtaccgc ccagccggtc aagtgatggc tgcgcgccgt cgccgccgat
     2941 gtatttctgc accatattga tgcggtccac cggcagatac aggcggtcgc ccgcttcata
     3001 ttcgagatgg aggaactcgc cttcgacagc ggcaaccttg aggaacttga ggccacgata
     3061 aacaccgatg ccgtggtcca ggtggacgac ataatcgtcc tgcttgagct cgcttaagct
     3121 cgacaaaaaa tgactcggat agctcttggt cgccgtggtc ggttgtcgct tacgtgtgcc
     3181 gaagatctca tcgaaagtga taaagatcag gtgctcctcg ggtaagcgga agccttggtt
     3241 taaattaccg cgtacgatgc cgcgcgcgat atcctggcgt tccagcacag cgggaagccc
     3301 atcctcaatc agcgccgcat cgatttgata gccgcccaag agttcgcgca agcgcatggc
     3361 gtcgcctttg gtcggcgcga cgaagataac tttttcggcg ctccaagatt tcaaacgttc
     3421 gaccagcggc gccagcgaaa catccttgcc atggagcgcc ggatcctggc gcaaatcggt
     3481 ggttaaaaaa gactcgacgt tgagcgtcgt ctcctgggct ttttccgcag gcgtgagcag
     3541 catcaacgct tcgccatgga ccgtggaaaa cggcatgagc gcctggcgcc actcatgttc
     3601 gttcaaatag agttgttcaa ccggcgcgac caggcgatgt tcttcgatgg ccaagcgatt
     3661 gcgctcccaa gcatgctggg cgaatcgttc gctttccgcc tcgatgcgat cggcgccatc
     3721 taaccagacg agagtgttgt ccggcagata cgaagtcaca ggcaccaagc cgggacaaaa
     3781 aaacggcaac aaaaattctc cgccggcaac cggtatgccc tcacggagcg attcgaggag
     3841 cgcctggcgc tcacgtcgat ttacttccaa ttccagggcg cgctgctcca aacggcgaat
     3901 agactcgtcc acgcccgcgc gcttgagcgc gaactctttc atcggcaaaa ggatcaattc
     3961 ttcctggacc gtctcggtgc gctgattggc ggggttgaat tcgcggatcg attccaagcg
     4021 atcattgtcg aattccaggc ggatcggccg gccgtaaccc ggcgggtaga gatcgacgat
     4081 gccgccgcgc acgctgaaat cgccgcgctc ctcgaccagc ggaacgtttt ggaaacccca
     4141 atggatcaaa tgatcgagca acgactcgcg tggcaagtct tggccggcaa cgatatagag
     4201 gtaagatttt ttcagggcct ctttgggaat cacccgctgc atgagcgccg ccggcgtggc
     4261 gatcagaatc ggcgccggtt cttcgacgag cttgtagagc ccttccaacc ggccggcgat
     4321 attttctggg tggggggaga gattttcaaa cgggagcact tcccaagacg gaaacagatg
     4381 gagtcggcgg cgcagcggcg ccaagccgcg ctcctcgccg agaaagaacg ccaaatcatc
     4441 gtagaggttc tccgcgtgat tggcgctagg cgcgatcacc agcaccggcc gctgaatctc
     4501 tgcggcagcg agcgcgatca ggtaggcacg cgcgccgccc tggatgcctt gaatgcgctt
     4561 actagcgctc cagtttccac gcaggaattg agcaagtctt tcgctgagcg gcgaaacggt
     4621 catgaaaaaa tactgaaacg ttggagccga atctttggaa tgttggcggc cgttggcgtg
     4681 gcccgggcgg cctcagccgc tttggcccag gcctcgacct ccgtgctgaa atcctttacc
     4741 cgtatttgca gatataccgg ctgataattc tccaagcgta taagcgcctg gtaaatcgct
     4801 cgcaccgagc ccttgacttc accgaagtcg acaaacatac gaaatgccgc ggctagttga
     4861 atcaagcctt cgagaaatgg cttagtaccc aactcgctgt cctggtaata accctcgaga
     4921 acctcgtggc actcaaagaa tttttgatcg ttaaatagac ttataccctc gcgcagccga
     4981 gcatccatat gtttgaaatg aataaagtag caacttccga cccacccctc aaggtgagct
     5041 gcgaggactt agaacagacg aaacaatttg agagatttga acgcttgatc agttcaatgg
     5101 cgcggccaca gccgctgatc gtactgcgcc acggccggaa cggcggcaag ccagatgcag
     5161 acatcggaga gcgccagcag caaagtggaa agcgtgaagg acatcgtata gctgccggtc
     5221 caatcgtgaa acacgccgcc gaagtaggac cccaacgctc cgccgacacc gacagccacg
     5281 gtggaatagc caagaatcga accgaagcct ttgccggcga agatatcggc cgtcagcgac
     5341 gaaaaaatga ccgcgcgcga gccgaagccg atgccgaaaa atattacgta gacgtagagc
     5401 aaccacgcct gactcggatc gcgcaggatc atcaaggcgc cgacaccgac aatggtcatt
     5461 gcgatattca atgaataagc cgcctgtttg gtcaagtggt cggcgataaa gccaaaaata
     5521 acccggccaa aactactggt aatcccggcg agaccgaaaa tcgacgccgc cagcagtttg
     5581 ctgaatccga cgtcgaccac atgcgccacc tggtgagtaa ctatcactgt agtaccggat
     5641 gccgcgccga tgcgggcgat gaacaacaac caaaactgca agctctgcaa ggcgagtttc
     5701 gcggtccact gattctgctc gcctttcttt tgcccgccat gagctccgct atggccatga
     5761 ggtccgggtt gataggaaag cagcacgatc ggcagcaaca aaaccaacac catggccgcg
     5821 taaatctcga acgcgcgctg ccatccatag gcgtaaatca gccatgcact gagcggcgcc
     5881 aacaacatga tgccgacgcc gctgccagcg taaaccacac cgatagccga agcgcggttg
     5941 gacgaaaacc actccgaaat taaaaaaata tgcggcacca ttccggtcaa ggtaaatccg
     6001 gtggcaagca cgaggccaaa gcaaatataa agttcccaga ggaccgagac ttggctgcta
     6061 agaaatagcc caactgccaa ggtgaggcag ccggcaatgg cgattctcct ggtgccgaag
     6121 cggtctagta agtatccagc ggcaggagaa agcagtgcat cgacaccaag gacaatcgaa
     6181 aatattcccg cggtggcccc ccggctccag ccgaaagaat cgagcaaggc gacattgaaa
     6241 acaccgaatg aagaatgcag cccgcgagag aatccaagta cgacgaatgc gaatgcggcg
     6301 attaaccaag gtgtcggtga gaaacgcatg acgataattg tctctcgtca ttaacagatg
     6361 gaagaaaggc ccacaagcaa acagcaataa tcacagtaaa actcgcacca cggcgcgtga
     6421 gagtcattgt cttttctcgc ctggacacct atcctgtcgt ctatccatga agatttgccg
     6481 ctggcttttc ctagcatttt gcctcgcgtt tactgcttgc gcaggttcga gcagcagctt
     6541 cttggcgggg cggcaagcac tgctacgcgg cgaaccggac aatgccgtca attatttcaa
     6601 tgctatcgcc caagctgatc caagttacat cgtcacgctg gcaccgccgc gcaagagtat
     6661 ctggacctat gtcggacggg cgcattacaa ctcgggcaga tacgcgcagg ccaaagatgc
     6721 cttcgataaa gcattaactc atcttaagga cgattatatc gcacagctat atcgcggctt
     6781 gactctcctt cggggttaca cctcgccgcc gccggctaat gcgatgacgt tgcaagaaat
     6841 ttcctacgcg ctgcgcg
//
LOCUS       DATLCH010000153         4138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2674094, whole genome shotgun sequence.
ACCESSION   DATLCH010000153 DATLCH010000000
VERSION     DATLCH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674094"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1041)
                     /locus_tag="VMZ02_04470"
     CDS             complement(109..1041)
                     /locus_tag="VMZ02_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71216.1"
                     /translation="MIGDSVTAAQSGKAERVGVIRLGGVLARVADGLRLGLKELGLED
                     GKQVVLNVHDLNGDAKGVEAVTHKFEQDKVKLIFTHTTPVTSVAVKATKNVPIVFAVG
                     TDPVGQGLIQSFGKPGGRLTGVQYLARDLTGKRMELLKEFLPSLRQIVTFYDPNNAVS
                     NQGATLGREAAQQLKLKLVEQRIKSPLELSNALVNIKPRQFDAYLYIADPMIASQAQL
                     IIDRARATKLPTMFHDQALVAGGALASYGQSYFEIGRRSAKYVQQVLAGTRPGDLPVE
                     TIDDVELAFNLTTAKEIGVTIPPNVLTRAAKVVR"
     gene            complement(1193..1558)
                     /locus_tag="VMZ02_04475"
     CDS             complement(1193..1558)
                     /locus_tag="VMZ02_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR71217.1"
                     /translation="MERKKILVVDDTEFNRDLVVQLLEEDYDLVSAENGEEALLMSEK
                     ERPDLILMDLGMPVMDGWEATRRLKANDALKNIPVIAVTSHAMVGDEVEARRAGCDDY
                     LPKPIDEDLLIKKIKTFIG"
     gene            complement(1596..3992)
                     /locus_tag="VMZ02_04480"
     CDS             complement(1596..3992)
                     /locus_tag="VMZ02_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR71218.1"
                     /translation="MKFRDLPIIRLFAGRQRGRLMRDYFLISVLLVGSGLVTSGAIEI
                     YYRYLESQETLALLQREVAAGAAFKIENFVQDIHSTLRGATRSREIAPKGLTNDFRFE
                     LEKLLLIAPSVTEAIALNQDGGIQVQASRLRTVLPDVKRDFATAPPFRQASKGQSYFG
                     PVYFVRGSEPYMTIAVPIERFAGDVIGVLQAEVNLKYIGDVVSNLKIGQAGYAYVISR
                     SGELIAHPDISLVLQRRNVLNLESTKAAFNPLDERQKRKALVTPNVQGKKVLTASAQV
                     NGLDWAVVVDRPVEEAYEPLYASVFRTSTVLLFGLGLALMASLFIGRRVVRPVGILRQ
                     GVERIGKGDLSYRLDLKTGDEIEVLADEFNKMTTQLQESYANLEQKVEDRTKELTESL
                     EQQTGTSQILGVIASSPTDIQPVLDVVAERAARLCDASDAQILRVYGETIRCVAGFGS
                     LPVVHDTPLKRTWPGSRAIIDRETVHIDDLAAVVATEYPDVKAFQEKWGHRTVLATPL
                     LREGVPIGAILIRRMEVRPFTEKQVALLRTFADQAVIAIENVRLFKELGEKNDQLEAA
                     NKHKSQFLANVSHELRTPLNSIIGFTRIVLRRTEGKIEQLQKDNLQKVLVSSEHLLSL
                     INELLDLAKIEAGRMEVYSENFKLEEVVRIATATVEPMLKNGNVKLVADLPADLPPLK
                     SDRDKLKQIVLNLLSNSAKFTEKGEIKVAAVRDNGTLKLTVSDTGIGMNQQALKYIFD
                     EFRQVDMSTTRKYGGTGLGLAIVKRLSNLLGGDIEVTSEEGKGSQFTVTIPMELKLPA
                     "
BASE COUNT          851 a         1122 c         1138 g         1027 t
ORIGIN      
        1 gcaatcgaga attgtcgatt accttgttct ccacttgaga acgtctctag cgtccgttgc
       61 agatgaagtt taatttagct cgtctctcgt cctacattag ttcagcgctc accgcacgac
      121 tttagcggcg cgcgtcagca cgttgggtgg gatcgtaacg ccgatttcct ttgcggtagt
      181 caaattaaat gccagttcca cgtcatcgat ggtctcgacg ggcaaatctc ccggtctggt
      241 gccggcgagc acctgttgta cgtacttagc tgagcgccgg ccgatttcga aataactctg
      301 gccgtagctc gccagcgcgc cgccggcgac cagcgcttga tcatgaaaca tagtcggcag
      361 ttttgtcgcg cgcgcacggt cgataattaa ttgcgcttgg ctcgcgatca tgggatctgc
      421 gatatagagg taagcgtcga actggcgtgg cttgatgttt accaaagcgt tactcagctc
      481 tagtggagat ttgatacgct gttcgaccag tttgagcttg agctgttgcg ctgcttcacg
      541 gcctaatgtt gcgccctgat tagacaccgc attgttcgga tcgtagaaag tgacgatttg
      601 ccgcaggctg ggcaggaact ccttaagcaa ctccatgcgc ttgccggtga gatcgcgggc
      661 caaatactgt actccggtca gacgcccgcc tggcttaccg aagctttgaa tgaggccttg
      721 gccaaccggg tcggttccga cggcaaatac gatcggcaca ttcttggttg ctttcaccgc
      781 tacgctagtc acgggcgtgg tgtgggtgaa aatcagtttg accttgtcct gttcaaactt
      841 atgcgttact gcctcgacgc ctttggcatc gccgttgaga tcgtgaacgt tgaggacaac
      901 ttgtttgccg tcctccagcc caagctcttt taggccaaga cgcaacccgt cagcgaccct
      961 ggccaacact ccaccgagcc gtataacacc gacgcgttcg gctttgccgg attgcgcagc
     1021 ggtgactgag tcgccaatca cggggatgat caacaaagcg aaaataacga aagtttttgt
     1081 tccgcgagac cgctttggaa gtgccgcttg acgccttaga gacgtcggaa gtcgctgcca
     1141 cgaaagcatc gtccggctag tgcctatgag tacgggtaac tggaatcaaa aatcacccga
     1201 taaaggtttt gatcttctta atcagcaagt cttcgtctat gggtttgggc agataatcgt
     1261 cgcaaccggc cctgcgtgct tcgacttcgt cgccaaccat ggcatgggaa gtaacggcga
     1321 tgaccggaat gttcttcagt gcatcattcg ctttgagccg tcgagtcgct tcccagccat
     1381 ccatcaccgg catacccaga tccatcaaaa tcagatccgg ccgttctttc tcactcatga
     1441 gcaaggcttc ttcgccgttc tcggcgctca ccaggtcgta atcctcctca agcagttgca
     1501 cgaccaagtc acgattaaat tcagtgtcgt cgacgacgag aattttcttt cgttccatgt
     1561 tactacctct tgttttgtgg atgacctttt gcgccttacg ccggcaattt cagctccatt
     1621 ggtatggtga cagtaaactg tgagcctttg ccttcttcgc tggtgacttc tatgtcgccg
     1681 ccgagcaggt tggagaggcg tttgacgatg gcgagaccca aaccggtgcc gccatatttt
     1741 cgagtcgtcg acatatcgac ctggcgaaac tcatcgaaga tatatttcaa cgcttgctga
     1801 ttcatgccga tgccggtatc ggagaccgtg agcttgagcg tgccattgtc gcgcacggcg
     1861 gcgactttga tctcgccctt ttcagtgaac ttggccgagt tgctgagcaa attgaggacg
     1921 atctgtttca gcttatcgcg gtcggatttc agcggtggca ggtccgcggg aagatcggcg
     1981 actagtttaa cgttgccgtt tttaagcatg ggttcgacgg tggccgtggc gatgcgcacc
     2041 acttcttcaa gcttgaaatt ctcggagtac acttccatgc gcccggcttc gattttcgcc
     2101 agatcgagca gttcattgat taggcttaag agatgctcgg aactcaccag gactttttgc
     2161 aagttgtctt tttgcagttg ctcgatcttt ccttctgtgc ggcgcagcac gattctggtg
     2221 aagccgatga tcgaattcaa cggtgtgcgc agctcgtggc tgacgttggc tagaaactgc
     2281 gacttgtgct tgttggcggc ttcgagctga tcgtttttct cgccgagttc cttaaacaac
     2341 cggacatttt caattgcaat cactgcctgg tccgcgaaag tgcgaagcag ggcgacctgt
     2401 ttctccgtga aagggcgcac ctccatgcgc cggatcagaa tggcaccaat gggtacgcct
     2461 tcgcgtagca gcggcgtcgc cagcacagtg cggtgtcccc atttttcctg gaatgctttg
     2521 acatccgggt attccgtagc gactaccgcg gcaaggtcat cgatatgaac ggtctcgcgg
     2581 tcaatgattg cccggctccc tggccacgtg cgtttgagcg gcgtgtcgtg cacgaccggc
     2641 agagatccaa agccggcgac gcagcgaatc gtctcaccgt agacacgcaa tatttgcgcg
     2701 tcactggcat cacacaagcg tgcggctctt tcggcaacga cgtcgaggac aggttggata
     2761 tccgtcggtg aactggcgat aacacccaag atttggctgg tacctgtctg ttgctccaat
     2821 gattcggtca gctctttggt gcggtcttcg accttttgct ccaagttagc atacgactcc
     2881 tggagctgcg tggtcatttt gttaaactcg tcggccaaga cttcaatctc gtcgccggtt
     2941 ttgagatcta accgatagct cagatcgcct ttgccgatgc gctcgacgcc ttgccgcaaa
     3001 atgcccaccg gccggactac tcgacggccg atgaagaggc tcgccatgag cgcaagcccc
     3061 aggccaaaca agagcaccgt ggatgtgcgg aagaccgacg cataaagcgg ttcgtaggct
     3121 tcctccacgg gtcggtcgac gacgacggcc caatctagac cgttcacctg agccgatgca
     3181 gtcaaaacct ttttgccttg gacgttgggt gtaaccagcg ctttgcgttt ctgccgctcg
     3241 tcgagcggat tgaacgcggc tttagtcgat tccaaattca gaacgttgcg tcgctgcaag
     3301 accagactga tatcggggtg ggcaatgagt tcaccgctgc ggctgatgac gtacgcgtac
     3361 ccggcctggc caatcttcag attagacacg acatcgccga tgtatttgag attgacctcc
     3421 gcttggagca cgccaatgac atcgccggcg aaacgctcaa tggggacggc gatagtcatg
     3481 tagggctcgg agccgcgcac gaaatatact gggccaaagt aggattgacc tttgctggcc
     3541 tggcgaaacg gcggcgcggt tgcgaaatcg cgcttgacgt caggcaatac cgtgcgcagc
     3601 cgcgacgctt ggacttggat gccgccgtct tggttgagtg cgatcgcctc ggtgaccgat
     3661 ggggcgatca acaaaagctt ttccaattcg aaacggaaat cgttggtcag ccccttgggc
     3721 gcgatctccc ggctgcgggt ggcgccgcgt agggtgctgt gaatatcctg aacaaagttt
     3781 tcgattttaa aagccgcgcc ggcggcgacc tcgcgctgca acagcgccaa ggtttcttgg
     3841 ctttcgaggt agcggtaata gatctcgatg gcaccgctcg tcactagccc actgccgacc
     3901 agcaggaccg aaatcaagaa gtagtcgcgc attagccggc cgcgctggcg tccggcgaaa
     3961 aggcgaatga tcggcaaatc tctaaatttc attatttaac tttgtcactc tcatctgtag
     4021 cacgagatgc gtggtttgtt cgagcaccat tatcgaggat gatcttagaa atcgtgccat
     4081 tttctcgaag ttctttgtcg gtcgaattaa tttggtgaac ggagttgccg ctttttgc
//
LOCUS       DATLCH010000154         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1185636, whole genome shotgun sequence.
ACCESSION   DATLCH010000154 DATLCH010000000
VERSION     DATLCH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185636"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VMZ02_04485"
     CDS             complement(<1..683)
                     /locus_tag="VMZ02_04485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71219.1"
                     /translation="MNIDIHAHFVPAESLKVASEIGKRQGLKLATNDIGREIVTRDGK
                     PFLTQPKAEFSNLDLRLSIMDSQSVDIQALSPASTYFFYWMAAEESLEFARWLNERLA
                     AAVAQHPKRLVALGSVPMQDCAKAVGELERAVTKLALRGVEIATNINGHYFDDPAFNP
                     FWEAAQALDALIFVHPNQVVGADRMKEFNLANLIGNPTDTSLAFAKLIFGGVLERYPN
                     LKFLLAHAGG"
     gene            956..1189
                     /locus_tag="VMZ02_04490"
     CDS             956..1189
                     /locus_tag="VMZ02_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71220.1"
                     /translation="MASKRRKDGRVTMREGDRILINKGIAKKVMIIARTEIPHSTERI
                     FVTKSGGKYTIGLKVWRSDYAWSDKNQMWVPKH"
     gene            1272..2636
                     /gene="holA"
                     /locus_tag="VMZ02_04495"
     CDS             1272..2636
                     /gene="holA"
                     /locus_tag="VMZ02_04495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HUR71221.1"
                     /translation="MKRDLATILKDLKSGKAPQLLLLFGDDLQVQESCRQIVAMLVPE
                     ESQAFNLERFDGRTVSWDQVEGSLMTPPFLPAKKVVWVENAPYFFSREQKGELGEKIL
                     ELWREGGRDAAVKLLADVLVVEGWTQERWERLDASSPDQLSEVFDYDGGTGQEEIAAL
                     LTFAKNRDLELSKRKAADSHRLGDLLDGKLPEWAFLLLDAVQVDRRTKLFKRLEELGA
                     TLFLGLERDRYGKVNRDSLINFILQRLRPAGKDIEPGARDMIVARAGDNLRMLQQEID
                     KLVLYSADHPIIRSKDVEAVVADNGEGWIFDLTRAVGDRDGLSALAQLGRLMGQGEHP
                     LKILGTIASEVRRLLAARQILDGDLRPFWKRGISYQQFQQIVIKQGTTALTRSPYADY
                     MCFQRAERFAMAELVSHMESLFEADLRLKSSRGQPRLILEKLILGMCLAASRNQPHGN
                     GATA"
     gene            2642..>3104
                     /locus_tag="VMZ02_04500"
     CDS             2642..>3104
                     /locus_tag="VMZ02_04500"
                     /inference="COORDINATES: protein motif:HMM:NF019509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HUR71222.1"
                     /translation="MLRPIIVVIFLAGLPSFCAAEMVVQACFSPQGKCSAYILREIDQ
                     AKKELLVAVYAFTNDELAGALVQAKKRGVAIQVVLDREFDARTENSKGKFFESNKIPL
                     RRLSGAKTQSQERDGGLMHQKFAVIDRKTVLTGSYNWTHSAEKANDENLLLF"
BASE COUNT          744 a          781 c          882 g          697 t
ORIGIN      
        1 ccaccggcgt gggcaagcag aaattttaaa ttgggataac gctcaagcac cccgccgaaa
       61 atcagcttgg caaaggccag tgaagtgtcg gttggattgc cgattaggtt tgccaggttg
      121 aattctttca ttcgatcggc accaacaacc tgattaggat gaacgaaaat gagcgcgtcg
      181 agagcttggg ccgcttccca aaaggggttg aacgccggat catcgaagta gtggccgttg
      241 atattagtgg cgatctcgac cccgcgcagt gcgagttttg tgaccgcccg ttcgagttca
      301 ccgaccgcct tggcacaatc ctgcattggc accgacccta aagccactag gcgtttggga
      361 tgctgcgcca ccgccgcggc caaacgttcg tttagccacc gggcgaactc cagggattct
      421 tccgcagcca tccaataaaa aaaatatgtg cttgccggcg atagcgcctg gatatcaaca
      481 ctctggctgt ccatgatcga taggcgcagg tcaaggttgg agaattcagc tttaggctgg
      541 gttaaaaacg gtttaccgtc tcgcgtaacg atctcacggc caatatcatt tgtggcgagt
      601 ttcagaccct ggcgtttgcc gatttcactg gctaccttca agctttcagc ggggacgaag
      661 tgagcgtgaa tgtcgatgtt catattttgc gctgacggat ctccggacag ctaccggagg
      721 gccggcaaat tgtcaagcaa aaccaccgat cagcggtttg gctcagtgcg acaaccatct
      781 gtgtgctttg cgaggcgcgt cacaacaaca ggttcgcgaa cttctcctga atgcatacta
      841 aggaaaaact acacttagtg tttagtccct gcgtcaggaa ggctttcgca ggatgttaag
      901 tccggtaacc catcaaatca ttgccagtcc aaattcggga tgatagcttt ggccaatggc
      961 cagtaagcgc aggaaagacg ggcgcgtcac gatgcgcgaa ggcgatcgaa ttctcatcaa
     1021 caagggtatt gccaagaaag ttatgatcat tgcgcgtacc gagattcctc actcaaccga
     1081 acgcattttt gtcaccaaat cagggggcaa gtacaccatc ggcttaaaag tatggcgctc
     1141 ggattacgcg tggagtgata aaaaccagat gtgggtgccg aagcattgaa cttgtcgcta
     1201 gatgtcggcc ggtatgttac cgcgatccgg ctaatcgttg tcttattgat tccattgggt
     1261 gcgcccgcgc tgtgaaacgc gatctcgcca ccattctcaa ggacctgaaa agcggcaaag
     1321 cgccgcagct attattgctt tttggcgacg atttacaggt gcaggagagc tgcaggcaaa
     1381 tagtcgccat gctggtgccg gaagaatccc aagcgtttaa cttagagcgt ttcgatggtc
     1441 ggacggtgag ctgggatcag gtcgaaggat cgctcatgac gccgccattt cttcccgcaa
     1501 aaaaagttgt gtgggtggag aacgcgccat attttttttc tcgcgaacaa aaaggtgagt
     1561 tgggtgaaaa gatccttgag ttatggcgcg aaggaggccg cgatgcggca gtgaaattac
     1621 tcgcggacgt attagtcgtg gaaggttgga cgcaggaacg ttgggagcgg ttggacgcga
     1681 gttcgccaga tcagttgtca gaggtatttg attacgacgg cggcaccggg caggaggaaa
     1741 tcgccgcgct gctgacgttt gccaagaacc gcgatctcga attgagcaaa cgcaaagccg
     1801 ccgacagcca tcgcttgggt gatctgctcg atggcaaact gcctgaatgg gcgtttttgt
     1861 tgctcgacgc cgtgcaagtc gaccgtcgga caaaattatt caagcgcttg gaagaactag
     1921 gcgcgacttt atttctcggt ctagagcgcg accgatacgg caaggtcaat cgcgatagtt
     1981 taatcaattt catcttgcag cggctgcggc cagcgggcaa agacattgaa ccaggggccc
     2041 gagacatgat agttgctcgc gccggcgata atttacgcat gctacaacag gaaatcgaca
     2101 agctagtgct ctactccgcc gatcacccga tcatccgatc taaagacgtc gaggctgttg
     2161 tcgccgataa cggcgagggc tggattttcg atctcacccg cgccgtcggc gaccgagatg
     2221 gtctaagcgc gctcgcccaa ttgggccgtc taatgggaca gggggaacat ccgttaaaga
     2281 tcctcggtac gattgctagc gaagtgcgcc ggttgctcgc cgctaggcaa attctcgacg
     2341 gtgacctgcg accattttgg aaacgcggca tcagctatca gcagtttcaa caaatcgtca
     2401 tcaagcaggg cacgactgcg ctgacgcgca gcccgtatgc cgattacatg tgtttccaac
     2461 gcgcggaacg gtttgcgatg gccgagctcg tgagccatat ggagtcgctg tttgaagctg
     2521 acctgcggct gaagtcgagc cgcgggcaac cgcgattaat actggaaaag ctcattctcg
     2581 gtatgtgttt ggccgcgtcg cgtaatcagc cgcatggcaa cggcgcgacc gcataatgac
     2641 tatgcttcgc ccgataatcg tggtgatttt tcttgcgggc ttgccgtcat tctgcgctgc
     2701 ggagatggtt gtgcaggctt gtttcagccc gcagggcaaa tgctcggcgt atattctgcg
     2761 tgagatcgat caagcgaaaa aagaactgtt ggtagcagtc tatgcgttta cgaacgacga
     2821 actcgcgggc gccttggtgc aggcaaaaaa gcgcggcgtc gcgattcagg tagttctcga
     2881 ccgtgagttc gatgcccgta cggaaaattc taagggtaaa tttttcgagt caaacaaaat
     2941 accgctgcgc cggctgtccg gcgcaaaaac gcaaagccaa gaacgcgatg gcgggttgat
     3001 gcatcaaaaa ttcgcggtta tagaccgcaa aaccgttctc accggctcat acaactggac
     3061 ccattcggcg gaaaaagcga acgacgagaa tcttctgctt tttc
//
LOCUS       DATLCH010000155         5737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_443989, whole genome shotgun sequence.
ACCESSION   DATLCH010000155 DATLCH010000000
VERSION     DATLCH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443989"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4992
                     /gene="uvrA"
                     /locus_tag="VMZ02_04505"
     CDS             <1..4992
                     /gene="uvrA"
                     /locus_tag="VMZ02_04505"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUR71223.1"
                     /translation="KGRLNLFLPQQDWRQEPFSNHLHCPHCDISYRDPVPNIFSFNSP
                     LGACETCKGFGRVIDIDLDLVIPDPGKSLAEGAIKPWINRKRRTKRLIDYCERKKIPT
                     KKPWAALTDAQRQLVVDGDGEYRGIRGWFRRLERRSYRMHVRVLLARYRAYLLCPECN
                     GTRLKVDALNYRIDGKDIAEINAMNVGVAHAFFTDLKPAGALDHVASLILDEIRRRTG
                     YLVGVGLEYLTLDRQSRTLSGGELERVDLTTAIGSSLVNTLYVLDEPSIGLHPRDSRR
                     LVEILHRLRANQNTVVIVEHDPEIIKECDYILDLGPKAGEQGGKVMFAGTYDELLKDE
                     NSLTAAYLSQRKSIPMPARRRKPVLQRLLKIERACANNLKNIDIEIPLGMLVCITGVS
                     GSGKSSLVDEVLTRNLKKLKEAPSANVTDCAAIKGIEKISEVVLVDQSPVGTTPRSNP
                     ATYMKAFDGIRKLFAGAELARLRGYTPSTFSFNVEGGRCETCRGEGFEKVEMQFLSDV
                     YTSCAECHGSRFREEVLQVSYRGKSIRQVLDLTVIDALDFFKDSNDIKNALEPLRAVG
                     LDYVRLGQPVTTLSGGESQRLKLAAHMARAKKAGTLFIFDEPTTGLHFHDIERLLWAF
                     NELIEQGHSVVVIEHNLEVIKCADHIIDLGPEGGDKGGEIVATGTPAQIAAVERSYTG
                     LYLRPYLTQSASPFAPLLKKDVSARSVLPTNIDDHSITVVGAKEHNLKNISVNIPRDR
                     FIVFTGLSGSGKSSLAFDIVYAEGQRRYLDSLSAYARQFLEVMARPNVDYVAGIPPTV
                     AIEQRLSQGGRKSTVATVTEIYHYLRLLYAKIGKQHCVNCGRPIHSLTRSQILDRTSR
                     SYRGKDVMVLSPLVRGRKGFHKEVIAGAVKLGYRRARIDGKLVDLRAPELVGGLERFK
                     EHNVDIVIGKAKAGGHEVEAMVDQGLRLGNGVIHLISNRGEQIFNQRLFCLGCGIGYE
                     PLDPRLFSFNSQQGACKDCGGMGFTYDFDPQLIFADPRRSVKHALAGISTGSSVNGSE
                     IERAMQRLLKEMAEDYEIDIAIPFAKQTKQVQEEIVVGGKGRGAFVGLVPYLKELAHA
                     GDENCANQLDELMTEVPCNACQGRRLNPRAQAVKVAGTTIWEVASYSVDEARGYFAKL
                     DLDHSDDATAARDRAVSDKVLKEIQQRLNFLAEVGLPYLTLDRRADTLSGGEAQRIRL
                     AAQLGSNLRGVCYILDEPTIGLHPRDNDMLLKTLRRLEQLGNSVLVVEHDEATIQAAD
                     LIIDLGPGAGIHGGNVVSIGTPAEIRVNPDSPTGAYLRSERKRLGPDRDLKVSQWLAI
                     RGAKAHNLQNLDVNIPLGMWSCITGISGSGKSTLVREVLYKGVKLLLGQFAGRPGQHK
                     EIIGWKALERIVEVDQTPIGKTPRSVPVSYIGVLDEIRKLYALAPEARLRGYTASRFS
                     FNVKGGRCEDCSGQGKIRKEMSFLPDVFIDCETCNGQRFNEETLSIRYNEKNIFDVFC
                     MTVEEAVPFFRAFPKIARPLKVLDDIGMGYINLGQASNTLSGGEAQRIKLAYELGKES
                     RGRTLYVLDEPTTGLHFLDVEKLIHILHRLVEMGNTVVTIEHNLDIVKEADYIVDLGP
                     EGGAQGGQLIACGTPREVIKAAKRSHTARFLREYLNGSIAPAAKKPLRIRATV"
     gene            4995..5387
                     /locus_tag="VMZ02_04510"
     CDS             4995..5387
                     /locus_tag="VMZ02_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HUR71224.1"
                     /translation="MAGRLFSMLGHGLRLRCPRCGVGRLYEKPFRMHDHCSRCTLKFE
                     REQGYFIGAIYINYIATVAIAVPGFFLLDAFTHATINQQLALWIPFALVFPLLFFHHS
                     RSLWLVFDHLFNPEKQLFRVPPKKTPND"
     gene            complement(5384..>5737)
                     /locus_tag="VMZ02_04515"
     CDS             complement(5384..>5737)
                     /locus_tag="VMZ02_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HUR71225.1"
                     /translation="KVSDMNRKVESFKDFVVDQLRDLEEIEARRMFGGYGLYRDDIFF
                     GIVHKGKLFFKIDEGTVGAYRKHKMKAFRPNAKQTLKSYYQVPVEIIEDADELCEWAL
                     RAVRCQEQNHHAQRL"
BASE COUNT         1275 a         1688 c         1534 g         1240 t
ORIGIN      
        1 aaaggccgct tgaatctgtt cttgccacag caggattggc gccaagagcc gttcagcaat
       61 catttgcatt gcccgcactg cgatatttcc taccgcgatc cggtgccgaa catattttca
      121 ttcaatagcc cattgggtgc ctgtgaaact tgcaaaggct tcggccgggt catcgatatc
      181 gatctcgatt tggtgattcc cgatccggga aagtccctcg ccgagggtgc catcaaaccg
      241 tggatcaacc gcaagcgccg gaccaaacgg ctgattgatt attgtgagcg caagaaaatt
      301 ccgaccaaaa aaccctgggc cgcactgacc gacgcccaac gccaactggt cgtcgatggg
      361 gacggcgaat accgcggcat tcgcggctgg tttcgccgct tggagcggcg cagctaccgc
      421 atgcacgtac gggtgttgct ggcgcgctat cgcgcctatt tgctctgtcc ggaatgtaat
      481 ggcacccggc tcaaagtcga tgcgcttaat tatcgcatag acggcaaaga catcgccgaa
      541 atcaatgcca tgaacgtcgg cgttgcccac gcattcttta ctgacctgaa accggcgggc
      601 gccctcgatc atgtcgcaag ccttatcctt gacgagattc gccgccgcac cggctatctg
      661 gttggtgtcg gtttggagta tctcaccctc gaccgccaat cgcgtacgct ttccggcggc
      721 gaactggaac gggtcgatct caccaccgcc atcggctcat cgctggtcaa cactctttac
      781 gtccttgacg agccgtccat cggtctacat ccgcgcgaca gtcgccgttt ggtggaaatc
      841 ctccaccggc tgcgcgccaa ccagaacacc gtggtgattg tcgagcatga tcccgagatc
      901 atcaaagaat gtgactatat tcttgatctc ggccccaagg ccggcgagca aggcggcaag
      961 gttatgttcg ccggcaccta cgacgagttg ttgaaggatg aaaactcgct caccgcggct
     1021 tacttaagcc agcgcaaatc gattccgatg ccggcacgcc ggcgcaaacc ggtcctgcag
     1081 cgattgctca aaatcgaacg cgcctgtgca aacaatctaa agaacatcga tattgaaatt
     1141 cccctgggaa tgctcgtgtg catcaccggc gtctccggct cgggtaagtc gagcttggtc
     1201 gacgaagtgc tgacgcgcaa tttgaaaaag cttaaagaag cgcccagcgc caacgttacc
     1261 gactgcgccg ccataaaagg aatcgagaaa atttccgaag tcgtgttggt cgatcagtca
     1321 ccggtcggca ccacgccgcg atcgaacccg gcgacttata tgaaagcctt cgacggcatt
     1381 cgcaagttgt tcgcgggcgc cgaactggcg cgcctgcgcg gctacacgcc gtcgactttt
     1441 tctttcaatg tcgagggcgg gcggtgcgaa acttgccgcg gcgaaggctt tgaaaaagtc
     1501 gagatgcagt ttctttccga cgtttacact tcctgtgccg agtgtcacgg tagccgcttc
     1561 cgcgaagaag tcctccaagt cagctaccgc ggcaaaagca ttcgtcaggt gcttgatctg
     1621 actgtcatcg atgccttgga ttttttcaaa gacagcaacg acatcaaaaa tgccctcgaa
     1681 ccgctgcgcg ccgtggggtt agactacgtt cgacttggcc agcccgtgac gacgctctcc
     1741 ggcggtgaat cgcagcgttt gaaactcgcc gctcacatgg ctcgtgcaaa aaaggccggc
     1801 acgctgttta tttttgacga accgactacc ggcctgcatt ttcatgatat cgagcgtctg
     1861 ctctgggcgt tcaacgaact gatcgagcag ggtcactcgg tcgtagttat cgagcataac
     1921 ttggaagtaa tcaaatgcgc cgaccatatt atcgacctcg gccccgaggg cggcgacaaa
     1981 ggcggcgaga tcgtcgcgac cggaacgccg gcacagatcg ccgccgtgga gcgttcatac
     2041 acgggcctgt atctgaggcc atatttgacc caatcggcgt cgccgttcgc gcccttattg
     2101 aaaaaagacg tctcggcaag gtctgtattg ccgacaaata ttgacgatca ttcgatcacc
     2161 gtcgtcggtg ccaaagaaca taacctaaaa aacatcagcg tgaatattcc gcgcgatcgt
     2221 tttatcgtct tcaccggact cagcggctcg ggcaagtcgt cgctggcgtt cgacattgtt
     2281 tatgccgagg gacaacggcg ctatttggac agcctgtcag cctacgcgcg ccagtttttg
     2341 gaagtgatgg cccgacccaa cgtcgactat gtcgcgggca ttccgcctac ggtcgccatt
     2401 gagcagcgct tgagccaggg cgggcgcaaa tcaacagtcg ccacggtcac cgaaatctat
     2461 cactacctgc gtctactcta cgccaaaatc ggcaaacagc actgcgtcaa ctgcggccgc
     2521 ccgattcatt cgttgacgcg cagtcaaatc ctcgatcgca cctcgcgttc ctaccgcggc
     2581 aaagatgtca tggtactaag cccactcgtg cgcggccgca aagggtttca caaggaagtc
     2641 atcgccggcg cagtcaaatt gggctaccgg cgcgcccgca tcgacggcaa gctcgtcgac
     2701 ctgcgcgctc ccgagttggt cggcggcttg gagcgtttca aagagcacaa cgtcgatatc
     2761 gtcatcggca aagccaaggc cggcggccac gaagtcgaag caatggtcga tcagggattg
     2821 cgcttaggca acggcgttat tcatctaata tcgaaccgcg gcgagcaaat tttcaatcaa
     2881 cgactcttct gtctcggttg cggcatcggc tatgaacccc tcgatccgcg gctattttct
     2941 tttaatagtc agcagggcgc ctgcaaggac tgcggcggta tgggctttac gtatgacttc
     3001 gatccgcagt tgatctttgc cgatccgcgc cggtcggtga agcatgccct cgccggcatt
     3061 agcaccggct catcggtcaa tggctccgag atcgagcgcg ccatgcagcg gttattgaaa
     3121 gagatggccg aggattacga gatcgatatt gctatacctt tcgctaaaca gaccaagcaa
     3181 gttcaggaag aaattgttgt cggcggcaag ggacgcggtg cctttgtcgg cctggtgccc
     3241 tacttgaaag aactcgccca cgccggcgat gaaaattgcg ccaaccaact cgacgaactg
     3301 atgaccgaag tgccctgtaa tgcctgccag ggccggcgct tgaatccacg cgcgcaagcg
     3361 gtcaaggtcg ccggcacgac catttgggaa gtcgccagct actccgtcga cgaggcgcgt
     3421 ggctatttcg ccaagctcga tctcgatcac tctgatgatg ccaccgccgc acgcgaccgc
     3481 gcggtgtcgg ataaagtgct taaagagatt caacagcgtt tgaattttct tgcggaagtc
     3541 ggtctcccgt atctaaccct cgatcgccgc gccgacacgc tttccggcgg cgaggctcaa
     3601 cgcatccggt tggcggcgca gttgggctcc aacctgcgcg gcgtctgcta catcctggat
     3661 gaaccgacta tcggtctgca tccgcgcgac aacgatatgc tgctgaagac tttgcgtcgc
     3721 ctcgaacagc tcggcaacag cgtgctggtc gtcgaacatg acgaagcgac cattcaagcg
     3781 gccgatctga tcatcgattt gggtcccggc gcgggtatcc acggcggcaa cgtcgtatcc
     3841 atcggcacac cggcggaaat cagagtcaat cccgattccc ccacgggtgc ctacctgcgc
     3901 tcggagcgca aacgtctcgg acccgaccgt gacttgaaag tctcacagtg gctcgcgatt
     3961 cgcggcgcga aggcgcataa cctgcaaaac ctcgatgtga acattccgct cggaatgtgg
     4021 agttgcatca cgggtatatc cggctcgggc aaaagcacgc tggtgcggga agtactgtac
     4081 aaaggcgtca agttattgct cggccaattt gccggccggc cggggcaaca taaggaaata
     4141 atcggctgga aagcgctcga gcggatcgtt gaggtcgatc aaaccccgat cggcaaaacg
     4201 ccgcgctcgg tcccggtatc ttacatcggt gtattagacg agatccgcaa gctctatgcc
     4261 ctggcgccgg aagcgcgtct acgcggttat accgccagtc gtttttcctt caacgtcaaa
     4321 ggcggccgtt gcgaggattg ctccggccag ggcaagattc gcaaggagat gagtttcttg
     4381 ccggatgtct tcatcgactg cgaaacttgc aatggccagc gattcaatga ggagactctc
     4441 agcattcgtt acaatgagaa aaatatcttc gacgtgtttt gcatgaccgt tgaagaggcg
     4501 gtgccgttct tccgagcctt cccgaagatt gcccgaccat tgaaagtcct cgacgacatt
     4561 ggcatgggtt acatcaatct cggccaagcc agcaacacgt tatccggcgg cgaagcccaa
     4621 cgcatcaagc tcgcctatga actcggcaag gaatcccgcg gcaggacgct ttacgtcttg
     4681 gacgagccca ccaccgggtt gcatttcctc gacgtggaaa aattgatcca tatcctgcat
     4741 cgactggtcg agatgggtaa taccgtcgtc accatcgagc ataacctgga catagtcaaa
     4801 gaagctgact atatcgtgga cttgggcccc gagggtggag cacaaggtgg tcaactgatt
     4861 gcgtgtggca caccgaggga ggtcatcaag gccgccaagc gctcgcacac cgctcgtttt
     4921 ctgcgcgaat atttgaacgg cagcatcgcg ccagcggcaa agaaaccctt gcgaatccgc
     4981 gcgaccgttt aaaaatggcc ggacgactct tcagcatgct cggccatggc ttgcgcttgc
     5041 gctgtccgcg ctgcggtgtt ggccgacttt acgaaaaacc gttccggatg cacgatcact
     5101 gctcgcggtg cacgctaaaa ttcgaacgcg aacagggcta cttcatcggc gccatctaca
     5161 tcaactacat cgccacggta gccatcgctg ttcccggctt ttttcttctc gacgccttca
     5221 cccatgctac gatcaatcaa caactagctc tctggattcc tttcgcctta gtctttccgc
     5281 tacttttctt ccatcactcg agaagcttgt ggctcgtgtt cgatcactta ttcaatccgg
     5341 aaaagcagct cttccgcgtt ccgccgaaga agacacccaa tgactaaagt ctttgcgcgt
     5401 gatgattctg ctcctgacaa cgcaccgctc tcaatgccca ctcgcacaac tcatcagcgt
     5461 cctcgataat ctccacgggg acttgataat aactcttgag tgtctgcttg gcgttgggac
     5521 ggaaagcttt catcttgtgc ttgcgatagg ctccaaccgt accctcgtcg atcttgaaaa
     5581 atagtttacc cttgtgaacg atcccgaaaa agatgtcgtc tcgatacaac ccatagccgc
     5641 cgaacatgcg ccgtgcttcg atctcttcaa gatcgcgcag ttgatcgaca acgaagtcct
     5701 taaacgattc aaccttccga ttcatgtccg aaacctt
//
LOCUS       DATLCH010000156         5304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6931731, whole genome shotgun sequence.
ACCESSION   DATLCH010000156 DATLCH010000000
VERSION     DATLCH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6931731"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VMZ02_04520"
     CDS             complement(<1..1073)
                     /locus_tag="VMZ02_04520"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR71226.1"
                     /translation="MQIDDLRDYLDILEEYQEVQRIHAEVDWNLEMGAITRRVYDLGA
                     PAALFEKVKGYPLGFRALGAPLGTSSRSGHGQFARTALAMHMPPSSSAKEIMSTYLQR
                     KEKLIPPETVKTGPCKENIDIGDAVDVLKFPIPLIHGGDGGRYIGTWHTVITKDPESS
                     WVNWGMYRLMVHDRNTLGCLFPMQQHIGQMYQKYEAMNKPMPVAVAIGGQPVIPLVSC
                     VMLPPYVNEADVAGALQNAPIPMVKCETVDLEVPASSEIVIEGEVLPHERRTEGPFGE
                     YMGYEAGKSSPKPILKIKAITYRNNPILPFSNMGMPVHEGQTATALIKGAEIYAELKK
                     LGLPVKSVFCPPFGVGHMCVVST"
     gene            1271..1678
                     /locus_tag="VMZ02_04525"
     CDS             1271..1678
                     /locus_tag="VMZ02_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HUR71227.1"
                     /translation="MAESNRPSGLPADLGAYLGAGTKINGKLHFEGQATIDGEVEGEI
                     VAQAHITVGQQAIVKGKVSAQSILVQGKVMADVIAEKKLEIQPPGSVVGDVTTQSLVI
                     GDGAILEGHISMKKNEGRVLPLRQEEKKQGAAS"
     gene            1954..2463
                     /gene="lspA"
                     /locus_tag="VMZ02_04530"
     CDS             1954..2463
                     /gene="lspA"
                     /locus_tag="VMZ02_04530"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HUR71228.1"
                     /translation="MSKLPRILLILYIILACIVCDQASKFIAKQFLQSGDFYSYAGDT
                     FRFQYAENNGAFLGLGATLPEPWRHLVFTIFVGAFLFALLAYLLRSRELTSFATVCLS
                     LVCAGGLSNLIDRLAYDGRVVDFLNVGIDSLRTGIFNVADMAITFGALLMLLESFRTS
                     KSPVVSAER"
     gene            2493..3044
                     /locus_tag="VMZ02_04535"
     CDS             2493..3044
                     /locus_tag="VMZ02_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71229.1"
                     /translation="MSRKGRKGIVGLVILSLWQAAAANRLFGEVHDQDTAPSLCRADE
                     QIFFACSVAPSRKNVSLCGSKSLDHRRGYLQYRYGKAGDIELQFPQAPANTQLVFRYA
                     HYFRARVDRTEISFDNHGYRYTIFDYYDGDAKPVIVTAGVRVGKYGARAAAIESLCLG
                     NSVRKLGRLETVIPRDPDNALNQ"
     gene            3093..3413
                     /gene="sugE"
                     /locus_tag="VMZ02_04540"
     CDS             3093..3413
                     /gene="sugE"
                     /locus_tag="VMZ02_04540"
                     /inference="COORDINATES: protein motif:HMM:NF008512.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HUR71230.1"
                     /translation="MAWIFLCVAGILEIFWAISLKYTEGFSNISWSVVTIIGMTASFY
                     FLAQALETIPVGTAYAVWTGIGAAGTAILGIILFSESAALARIACIGLIVAGIIGLKF
                     TSSS"
     gene            3498..3911
                     /locus_tag="VMZ02_04545"
     CDS             3498..3911
                     /locus_tag="VMZ02_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HUR71231.1"
                     /translation="MSTASNVTALKEAYKRWHESKAGSVDHWLHLMTDDVKFRSLAEG
                     AIQMQFTRPSSNKAEVEQYFKGLTNEWIMNDYRVDEYIAEGERVVALCHVSFKHRKTG
                     KTLVTPKADFHKFRDGKICEFFEFYDTAKALAATL"
     gene            4000..4917
                     /locus_tag="VMZ02_04550"
     CDS             4000..4917
                     /locus_tag="VMZ02_04550"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71232.1"
                     /translation="MIIDCHAHLVPQSWYHPQSPKWMFDLPHLFDEQDKSGVDITVFG
                     NNWIRSPDGHDPLKVVQEFNTHAAELTAKHPNRLLGLACSVPYDSGPILKETEKAIRE
                     YKLKGVMINSSTDGEYLDSPRATPFFEMVSELNIPVFVHPPKFTIGNEKMEINRLPEL
                     LGRPFDTTLSLTRFIFTGGFEKFPKLKLVCAHVGGALAMLPGRYGFGYELKKDNNFGP
                     WEPDVMTRPPASYMKQLFFDTVCYHPPAVQCAIDTIGIDQMVFGSDTPPVPITLVRAV
                     DTVKQLKISDADKQKILGDNAAKLLGLAG"
     gene            4969..5103
                     /locus_tag="VMZ02_04555"
     CDS             4969..5103
                     /locus_tag="VMZ02_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71233.1"
                     /translation="MARQINPGDPFPTFNVSLTDGRTLMLPGDLDGQYAVIIYYRGIW
                     "
     gene            5113..>5304
                     /locus_tag="VMZ02_04560"
     CDS             5113..>5304
                     /locus_tag="VMZ02_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71234.1"
                     /translation="MRQVADYQTHLEEFKAENTDIIALSVDPLDKAQEMVARTGAKFP
                     IGYGLKVPEDADRVGAYWEE"
BASE COUNT         1260 a         1462 c         1356 g         1226 t
ORIGIN      
        1 tcggtagaca cgacgcacat atgaccgacg ccaaagggtg gacagaacac gcttttgacc
       61 ggcaaaccga gtttttttag ttctgcatat atttccgcgc cttttatcag tgctgtcgcg
      121 gtctgccctt catgcaccgg catgcccata ttggagaatg gcaagatcgg attattacgg
      181 taggtgatcg cttttatctt cagtatcggt ttcggcgacg acttgccggc ttcgtaaccc
      241 atatactcac cgaatgggcc ttcggtcctc ctctcatgcg gcaacacctc gccctcgata
      301 acgatctccg aggatgccgg cacttcaagg tcgacggttt cgcacttcac catgggaatc
      361 ggcgcattct gcagggcgcc ggcgacatcc gcttcattga cgtaaggcgg taacatgacg
      421 caactcacca gcggaataac cggttggccg ccgatcgcca cggccactgg cattggttta
      481 ttcatagctt cgtacttttg atacatctgc ccgatgtgct gctgcattgg aaaaaggcat
      541 ccaagagtat tacggtcatg caccatcagc cggtacatac cccagttaac ccacgagctc
      601 tccgggtctt tggtaatcac cgtgtgccaa gttccgatgt agcgtccgcc atcgccgcca
      661 tgtatcagcg gaatcggaaa cttgagcacg tcgacggcat cgccgatgtc gatgttttct
      721 ttgcacggac cggtcttcac cgtctccggt gggataagct tttccttgcg ctgcaagtag
      781 gtagacatga tctctttggc ggaggatgac ggcggcatat gcatggcaag cgctgttcgc
      841 gcgaactgac catggcccga acggctactc gtccctaaag gtgcgccgag tgcgcgaaag
      901 cccaggggat aacctttcac cttttcaaac aacgccgccg gcgcgccaag atcataaacg
      961 cgccgggtaa tcgcgcccat ctccaagttc caatctactt cggcgtggat gcgctgcacc
     1021 tcttgatact cttccaggat gtcgaggtaa tccctcaaat cgtcaatttg catttgtcac
     1081 tccaattcta gaatcggcag gcggaagccg ggctcagcgg caagtttacg ataggtaatt
     1141 tatagcagag caagttcacc gcggcaaaag aaatccccgc agcttttgac gaccggccag
     1201 ctcgcggaaa aactagacaa aaaaatcccg gtgatctata gtccggagct tgaccggaaa
     1261 ggagacaata atggcagaga gcaacagacc aagcggcttg ccggcggatt tgggcgcata
     1321 cctcggtgcc ggcacaaaaa ttaacggcaa acttcacttt gaaggccaag cgacgatcga
     1381 cggcgaagtc gaaggtgaaa tcgttgccca agcgcatatc actgttgggc agcaggcgat
     1441 cgttaaaggc aaggtttccg cccagtcgat attagtccag ggcaaggtca tggcggatgt
     1501 gattgccgag aaaaaactcg aaattcaacc gcctggctcg gttgtcggcg atgttaccac
     1561 tcagagcttg gtaatcggcg acggtgccat tctcgaaggc cacatctcga tgaagaaaaa
     1621 tgaaggtagg gtcctaccgc tgcgccaaga ggaaaaaaaa caaggcgcag cttcctaagt
     1681 ttcagcgaca attcggcatt tttgcgcgcc ttaaagcgca cgcttatccg gcgccatacg
     1741 atcacagcgg caagagttca atggcaaatg ggtttgtcgc aggacaagcc catttctttt
     1801 attccgcatt ggcgtcgccc cactgctttc ccacttccca ggagggataa agaagatctc
     1861 tccgtctgaa aactaaagtt tctccagaag ctctccgtca gcgaagtttg cccggttgta
     1921 acctgcagta agaaaagcta cgaaaaagcg agtatgagca agctgccgag aattctttta
     1981 atcctttaca tcattctggc ctgtatcgtt tgcgaccaag cgagcaaatt catcgcaaag
     2041 caattcctgc aatccgggga tttttattcg tacgccggcg acacgttccg ttttcaatac
     2101 gcggaaaata acggcgcctt cttgggtctc ggcgcgactc tacccgaacc gtggcgccat
     2161 ttggttttta cgattttcgt cggggcattt cttttcgcgc tgctagctta tctcttacgc
     2221 agcagagagc taacgtcgtt tgcaaccgtc tgcttatcgc tcgtttgcgc cggcggcttg
     2281 agcaacttga tcgaccggct cgcctacgac ggtcgtgtcg tcgattttct taatgtcggc
     2341 atcgactcgt tgcgcaccgg tatcttcaac gtcgccgaca tggcgatcac gttcggcgca
     2401 ttgctgatgc tgctggaaag ctttcgcacg tcaaaatcgc cggtagtttc ggctgaacgc
     2461 tgatcgagtt gtcttacgta aacgagcaaa ctgtgtcgcg caaaggtcgc aaaggtatcg
     2521 ttgggctagt catactctcg ctctggcaag cagctgcagc caatcgactg ttcggcgaag
     2581 tacacgatca ggacactgca ccatcgctgt gccgtgccga cgaacaaatc ttcttcgctt
     2641 gttcggtggc accttcgaga aaaaacgttt cgttatgcgg ctccaagtcg ctcgaccatc
     2701 ggcgcggcta tctgcaatac cgctacggta aagctggcga catcgaactg cagtttcctc
     2761 aggccccggc caatacccaa ctcgtcttcc gctatgcaca ttactttcgt gctcgggtgg
     2821 accgcaccga gatcagtttc gacaaccacg gatatcgtta taccatcttc gattattacg
     2881 acggcgacgc taagccggtg atagttacgg ccggtgtccg tgtcgggaaa tatggcgcaa
     2941 gggctgcggc aatagagtcg ctctgcctgg gcaattcggt ccgtaaactc ggcagactag
     3001 agacggtgat tccgcgtgac cctgacaacg cgcttaatca ataataatat ctcgttcacc
     3061 ggatcagcat ctttcgttgc taggagttct tgatggcgtg gattttttta tgcgtcgccg
     3121 ggattttgga aatcttctgg gcaatcagcc tgaagtacac cgaagggttc agtaatatct
     3181 cgtggagtgt tgtaacgata atcggcatga ctgcaagctt ctattttctc gcccaagccc
     3241 ttgagacgat tccagtcggc actgcttatg cggtttggac cggtatcggc gccgccggca
     3301 cggccatcct gggtattatc ctcttttccg aatcggccgc tctagcgcga atcgcctgca
     3361 tcggcctaat cgtcgccggc atcatcggac tgaaattcac atcctcgtct tagtcttagc
     3421 cggctaggaa tatgtgccct caaccgcaga aatctgctat gtaagattcg tataccgaca
     3481 aatggaagga gcaaattatg agcacagctt caaacgtaac ggcattaaag gaggcctaca
     3541 agcgctggca tgagagcaag gccggcagcg tcgatcactg gctgcacctg atgaccgacg
     3601 acgtgaagtt ccgttcgctc gccgagggag ctatccaaat gcagttcacc cggccctctt
     3661 ccaacaaagc cgaagtggag caatatttta aaggcctcac caacgagtgg ataatgaacg
     3721 attaccgtgt tgacgaatac atcgccgagg gggagcgtgt cgtcgccctt tgccacgtat
     3781 cgttcaaaca caggaaaacc ggcaaaacat tggttactcc caaagccgac tttcacaaat
     3841 tccgcgacgg gaaaatttgt gagtttttcg aattttacga taccgcaaaa gcgctagcag
     3901 cgaccctatg acccataact tggtcgagac tagcaatagt aattcattct cgactttcca
     3961 ccgttagtca tatgctctcc aaagaaaggt tctgacgcaa tgatcatcga ttgtcacgct
     4021 catttagttc cccaaagctg gtatcacccg caatcaccca agtggatgtt cgatctgccg
     4081 cacttgttcg atgaacagga taaatccggc gtcgacatta cggtgtttgg caataactgg
     4141 atacgttcgc cggacggcca tgatccgttg aaggtcgttc aggagttcaa tactcacgcc
     4201 gccgagctca ctgctaagca tcccaaccgt ctgctcggcc tcgcttgctc cgtcccgtac
     4261 gacagcgggc cgattctcaa agaaaccgag aaagcgatcc gcgagtacaa gctcaaaggc
     4321 gttatgatca attcgagcac cgacggcgaa tatctcgact cgccacgggc gacgccattt
     4381 ttcgagatgg tcagcgaatt gaatattcca gtgtttgttc atccacccaa attcaccatc
     4441 ggcaacgaga agatggaaat aaaccgcctg ccggagctgt tggggcggcc cttcgacacg
     4501 acactgtcgc tgacgcgctt catctttacc gggggcttcg aaaagtttcc caaactcaag
     4561 ttggtttgcg cccatgtcgg cggcgcgctg gcgatgctgc cgggccgcta tggcttcggt
     4621 tacgagctca aaaaagataa caactttggc ccgtgggaac ccgatgtcat gacccgcccg
     4681 ccggcgagtt acatgaaaca actgttcttc gacactgtct gctatcatcc gcccgcggtg
     4741 cagtgcgcca tcgataccat cggcatcgat caaatggttt ttggcagcga cacgccgccg
     4801 gtaccgatca ctctggtgcg cgcagtagac acggtaaagc agttaaagat ttccgacgcg
     4861 gacaagcaaa aaatattggg cgacaacgcc gccaaattac tgggcttggc gggttaattg
     4921 acaccggttc acatacgccc aggacaaatt gaccgggaag aggattacat ggctagacag
     4981 atcaatcctg gagatccgtt tccaacattc aacgtaagtt taactgacgg ccggacgctc
     5041 atgcttcccg gcgatctcga cgggcaatac gccgtgatca tttactatcg cggtatctgg
     5101 tgaccctatt gcgtgcggca ggtggccgac tatcaaactc atctggaaga attcaaagcc
     5161 gagaataccg acatcatcgc cctttccgtc gatccgctcg acaaggccca ggagatggtc
     5221 gcaagaaccg gcgcgaaatt tcccatcggc tacggcctta aagtgcccga agatgccgac
     5281 cgggtcggcg cctactggga agaa
//
LOCUS       DATLCH010000157         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1191376, whole genome shotgun sequence.
ACCESSION   DATLCH010000157 DATLCH010000000
VERSION     DATLCH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191376"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VMZ02_04565"
     CDS             <1..483
                     /locus_tag="VMZ02_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUR71235.1"
                     /translation="QKMLEDIARRSIVRPKQAPVPPSTGRGLDSMLGQMDWPVKGAVS
                     APFGKFKHPEFAAELVRKGIDIDAALGDGVRAVEKGRVVFADRFTGYGRMVILDHGER
                     YYTIYAHLSEIVKKNGEEVQRGDILGRVGDSDSLSGAKLYFELRKDGRSVDPLPWFKK
                     "
     gene            550..1911
                     /locus_tag="VMZ02_04570"
     CDS             550..1911
                     /locus_tag="VMZ02_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HUR71236.1"
                     /translation="MQLVKRHGTPLLLIVLGIGIGFFLSAQWVPNVSAVGRQDYESLE
                     AFSNILSIVKKNYVEDIETKNLVNGAINGMLSSLDPHSAYLTPDLYKDLQSDTQGRFG
                     GLGIEITVKSGILTVVSPIEDTPAFKAGIKPGDQIFKIEDEFTKDMSLVDAVKKMRGL
                     KGTKINLTVRREGAAELIDVSLVRDIIRVQSVKSRTLEPGYGYVRLAQFQERSDRDVQ
                     RALEKLAAEKNGIKGLVLDLRNNPGGLLNQAVRVSDLFLDSGMIVYTDGRIESQKQKF
                     FAQKDGTWGDFPLVVLVNGGSASASEIVAGALQDHKRAVVLGTKTFGKGSVQTILPLD
                     DNSALRLTTARYFTPKGRSIQATGIVPDIVIENIVLQDGKPDDRKRPSLREENLPGHL
                     NNPQQNSAKEQELIDREREKQIPTSAPTGDETIDNDQQLKRALDLLKSWDVFKQVVQK
                     KAA"
     gene            1984..>2804
                     /gene="xseA"
                     /locus_tag="VMZ02_04575"
     CDS             1984..>2804
                     /gene="xseA"
                     /locus_tag="VMZ02_04575"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HUR71237.1"
                     /translation="MARPTANELELPLFASQLRKFLTVSELNERIKGTLESRLDGLWV
                     QGEISNFRMPPSGHFYFTLKDDKSQISAVMFRRQGGMLRFTPDNGMAVLCFGRVSVYT
                     VRGDLQLYVDDMEPQGQGALALAFEQLKKKLAAEGLFGNARKRELPFLPHCIGIVTSG
                     KGAALQDILRIIGDRYPDRRIVVCPVKVQGDGAAREIAEAIFALNQVAAVEVIIAGRG
                     GGSLEDLWAFNEEIVARAIFASHVPVVSAVGHEIDFTIADFVADARAPTPTAAAE"
BASE COUNT          650 a          707 c          802 g          645 t
ORIGIN      
        1 caaaaaatgc ttgaagacat tgcgcgcagg tcgattgtcc gaccgaaaca agcgcctgtg
       61 ccaccttcga cgggccgcgg tcttgactcc atgcttggcc aaatggactg gcctgtaaaa
      121 ggagcggtga gcgcgccatt cggcaagttc aaacatccgg agtttgctgc cgagcttgtc
      181 cgcaagggca tcgatatcga tgccgcatta ggcgatggcg ttcgcgccgt ggaaaagggc
      241 cgcgtggtat ttgccgatcg gttcaccggc tacggccgta tggtgatcct cgatcatggc
      301 gagcgttact acacaattta cgctcatctt tccgaaattg ttaaaaaaaa tggcgaagaa
      361 gtgcaacgag gagatatcct cggacgggtc ggagacagtg attctctgag cggtgcgaaa
      421 ctctatttcg agttgcgtaa ggatggtcgg tccgtagatc ctctaccgtg gttcaagaag
      481 tgatagctat tccaaaaaca ttgagttaga cttagccaaa gttgtcagca aattttaggg
      541 ggaaacttca tgcaacttgt caaaagacat ggaacgccat tgctactgat cgtgctcggc
      601 atcggcatcg gttttttcct gtccgctcag tgggtgccga atgtatcggc ggtcgggcgg
      661 caggattacg agagtttgga ggccttcagt aacattcttt cgatcgtgaa gaaaaattac
      721 gtcgaggata tcgagaccaa aaacttggtc aatggcgcca ttaacggcat gttaagttcg
      781 ctggatccgc acagcgcgta cttgacgcct gatctgtaca aagatttgca gagcgacacc
      841 caaggacgtt tcggtggttt gggtatagag attaccgtca aaagcggcat tctcactgtc
      901 gtttcgccca tcgaagatac ccccgctttc aaagccggca tcaagccggg tgatcagatt
      961 ttcaaaatcg aagacgaatt caccaaggac atgtcgctcg tcgatgcggt aaaaaagatg
     1021 cgcggcttga agggcacgaa gattaatcta acggtgcggc gcgaaggcgc agccgagtta
     1081 atcgatgttt ctctggttcg tgacatcatt cgagttcaaa gcgtcaagag ccgcacctta
     1141 gagcccggtt atggttatgt tcgtttggcc cagtttcagg aacgtagcga tcgcgatgtg
     1201 cagcgcgcct tggaaaaatt ggcggccgaa aaaaacggca tcaaagggct tgtacttgat
     1261 ctccgcaaca atcccggcgg cttgttgaat caggcggtgc gggtgtcgga tctattcctg
     1321 gattccggca tgatcgttta caccgatggc cgcatcgaat cgcaaaaaca aaaattcttc
     1381 gcccaaaagg acggcacttg gggagatttt ccgctggttg tcttggtcaa tggtggtagc
     1441 gccagcgctt ccgagatcgt cgccggcgcg ctgcaagacc acaaacgggc ggtggtgctc
     1501 gggactaaaa ctttcggcaa gggctcggtc cagacgattt tgccgttgga cgataattcg
     1561 gcgctgcggt tgaccacggc gcgatatttt acgcccaagg gtcgatcgat tcaggccacc
     1621 ggcatcgttc ctgatatcgt gattgaaaac atcgtgcttc aggatggcaa accggacgat
     1681 cgcaagcggc caagtttacg cgaagagaat ttgccgggcc acttgaataa cccgcagcaa
     1741 aactccgcta aagaacaaga gctgatcgat cgagagcgag agaagcagat tccgacctcg
     1801 gcgcccaccg gtgacgagac catcgacaac gatcagcagc tcaaacgcgc tctcgacctg
     1861 ctgaagagct gggatgtctt caagcaggtg gtgcagaaaa aggctgcata gtctttaatt
     1921 atgcgtcgat aaaaaggccc tcccctggag ggtctttttt tttgtgttac aatcgcccgc
     1981 aatatggcgc gtcccactgc caacgaactc gaattaccac ttttcgccag ccagttacgc
     2041 aaatttctca ctgtcagcga actcaacgag aggatcaaag gcacgttgga gtctcggctc
     2101 gatgggctat gggtgcaggg cgaaatctct aactttcgta tgccgccttc gggtcatttc
     2161 tatttcactc tgaaagacga caagagtcag atttcggccg tgatgtttcg ccggcagggc
     2221 gggatgctgc gctttacgcc tgataacggt atggcggtgc tctgctttgg ccgcgtgagc
     2281 gtgtacacgg tgcgcggcga tttgcagctt tacgtcgacg acatggagcc gcaagggcag
     2341 ggcgcattgg cgctggcttt cgaacaattg aaaaagaaac tcgccgccga aggattgttt
     2401 ggtaacgcaa gaaagcgcga gctgccgttc ctgccgcact gcatcggtat tgtcacttcc
     2461 ggcaaaggtg ccgcattgca ggatatactg cgtatcattg gcgaccgcta tcccgaccgg
     2521 cgcatcgtcg tttgcccggt taaggttcaa ggcgacggcg ccgcgcgcga gattgctgaa
     2581 gcgatttttg ctcttaatca ggtcgccgct gtcgaggtga ttatcgccgg ccgaggcggc
     2641 ggttctttgg aagatctgtg ggcctttaac gaggagatcg tcgccagggc catttttgca
     2701 tcgcacgttc cagtggtttc ggctgtcggt catgagatcg actttacgat agccgacttt
     2761 gtcgccgatg cgcgcgcgcc gacacccacg gcggccgccg aaat
//
LOCUS       DATLCH010000158         7053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2681243, whole genome shotgun sequence.
ACCESSION   DATLCH010000158 DATLCH010000000
VERSION     DATLCH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681243"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /gene="trpE"
                     /locus_tag="VMZ02_04580"
     CDS             <1..1358
                     /gene="trpE"
                     /locus_tag="VMZ02_04580"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HUR71238.1"
                     /translation="AFILESVEGSERWARYSFLGTRPQRIFKARGNQVELTENGKTRK
                     WQSDSPLTALQELLKDFRPVPVPGVPPFFGGALGYVSYDAVEQFHGIVNRKKDPLGMP
                     EIFFLFVQTLIAFDNLKHTIKVIDNVQVDPGTNLRRAYDGAVATIHKVISTLQKKPRG
                     IEPRDLSPASGKRKFRSNLTPQAFQEAVTRAKEYIKAGDIIQTVLCQRLETETDSDPF
                     EIYRALRFVNPSPYMYYLEMEDLRVIGSSPETMVRLTGETIELRPIAGTRRRGTTPEE
                     ERDLESELLADPKERAEHIMLVDLGRNDVGRVAAIGSVDVNELMAIERYSHVIHIVSN
                     VRGKLAPDKTPFDLFVSAFPAGTVSGAPKIRAMQIIAELEPQKRSLYAGAIGYFGYNG
                     NLDTCIVIRTIVMKGKKVFINAGAGIVADSDPVLEYQETLAKARAMLRAVELAEQWRK
                     R"
     gene            1355..1921
                     /locus_tag="VMZ02_04585"
     CDS             1355..1921
                     /locus_tag="VMZ02_04585"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HUR71239.1"
                     /translation="MILLIDNYDSFTYNLYHYLGELGATVRVVYNDKISLEEIAALRP
                     EKIVISPGPCTPKQAGISCDVINRFGAQTPILGVCLGHQAIGAAYGGRIIRAPTVMHG
                     KLSAVSHDFKTVYRGLKNPFAAMRYHSLVIDPESLPVDLLVSARTDDGIIMGVRHKTL
                     PVEGVQFHPESILAEEGKKLLKNFLDSY"
     gene            1925..2344
                     /locus_tag="VMZ02_04590"
     CDS             1925..2344
                     /locus_tag="VMZ02_04590"
                     /inference="COORDINATES: protein motif:HMM:NF020489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HUR71240.1"
                     /translation="MKLLCFQAKRFHWKTHSKTLPEAADQTIDQEVSESVVVFLHAEA
                     ADGDGERNVSVMRQTLKHIKWLANKRELKTIVLHSFTHLGGVTAPAGLAETLIVTLQQ
                     RLSETGYQVWTTPFGYFCEWDLSVYGESLAKVWKEIH"
     gene            2432..2989
                     /locus_tag="VMZ02_04595"
     CDS             2432..2989
                     /locus_tag="VMZ02_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HUR71241.1"
                     /translation="MTANTKLNCWIIAAAIFLLVPSCKKTDQAASPDDLPDLNKQIRP
                     YVTTDNAKIRTGPGPQFRGIADVPANAKVHVVGRDGDWALIVSKKGNAPGFIELAAIK
                     PGEGEPEKPEFGEPAKVEGKYEALADTQVRSGPGLHYPSVADIAKGTKLNVVDEQKGW
                     LKVESKRGNKPGYVESSLAKPVNEK"
     gene            complement(3006..3974)
                     /locus_tag="VMZ02_04600"
     CDS             complement(3006..3974)
                     /locus_tag="VMZ02_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71242.1"
                     /translation="MVVDFQHHYIPVELAKKRGLYSETGRTMLQEGGLPATTMHQRLY
                     DLELQLKDMDEAGVDVSVLSCLLGWTAPMEECRFINDDLAAIQKRFPGHFVGLAQAPI
                     LEGEAACTELRRAITELGLRGLTITSQVNGLSLDSPKLYGLYELACELDAPVFVHPAL
                     VPTGYEHLQDYDLPRVLGREVDLTVATTRLIAGGIYDRYPNLKIVMAHFGGGIAAVKD
                     RLVGKGYRFGTLKKPFAEYFDMVYFDMAGFEGGTTALRCALEGIRPERLVFASDYPQD
                     FTGVNTDTGKGMTELRNYVAAVRKLPLNEKHREDMLGGTAVRLLKL"
     gene            4606..5226
                     /locus_tag="VMZ02_04605"
     CDS             4606..5226
                     /locus_tag="VMZ02_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HUR71243.1"
                     /translation="MENIYGEDISVSKGTAAFKGNFAPLTNSFVSNIGQPSSPGMYLR
                     NSANKPIVLDFGTNGISSFSVDWKLFKNGKGLTILADGIIVDQQTLLKSQKKSGAGGN
                     LTLYFDDAVHQLQFVGLKNTRFGLDNLLINLPEPDAGGLETSSLTEESNGSNEGQGDP
                     GERINPLENIVPLVDTTASVPEPASLVLLVVGLCAAWWSRRYAVSS"
     gene            complement(5331..6764)
                     /locus_tag="VMZ02_04610"
     CDS             complement(5331..6764)
                     /locus_tag="VMZ02_04610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71244.1"
                     /translation="MISPHQTTAPGAALSIAEERAILSVACLAAFLFFNSFGSISVAL
                     PTIQKQFGNSLSEIQWITLMGVVTISSLSFCFGRAGAILGQRRLYKIGVALYATGAGL
                     GALAVSFSQLLMARAVMAVGLAMALPMSTALLASSFANERRGQVLGTFASAIAVGRMT
                     GPSVGGFLLQLGGWTWIFWMNFVVGSAVTVAVIKIFRGAGEQRHEQFDIWGSVALLIG
                     YPSLLIGLSFGAALGWNSPWVISWFAVAAAGLASFVAIERHTQRPLVDVTIFRNKALS
                     AAMIAVILSHMIHNPIALAAPLYLQNVLGASALGAGFLLAILPLSTALLSPLSGRLAD
                     RFDATSVSAVGIGLIVVGIAGYACLGVDSHFAWVGAVLGLLGAGIGVFTPANQKVAFG
                     SVTQNDYGVLAAMLSSFGTAAGTIGTTIAVALMEIDGGPKLWSSRQALAGAQQFAFTW
                     LVPLGLIALIVGVCQSHGSNRAGPFKS"
BASE COUNT         1747 a         2024 c         1824 g         1458 t
ORIGIN      
        1 acgcgtttat tttagagagc gtcgaaggca gcgagcgctg ggcgcgttac tcgtttctcg
       61 gcacccgtcc gcagcggatt ttcaaagccc gtggtaacca agtcgagctg accgaaaacg
      121 gcaaaaccag gaaatggcaa agcgactcac cattgacagc gttgcaagaa ctgctgaagg
      181 atttccgtcc cgtgccggtg cccggtgtcc cgcctttttt cggcggcgcg ttgggctacg
      241 tatcttacga tgccgtcgaa caattccatg gcatcgtcaa tcgtaaaaaa gatccgctcg
      301 gcatgcccga aatctttttc ttgttcgtgc aaaccttgat tgccttcgac aatctcaagc
      361 acaccatcaa agtgatcgat aacgtccagg tcgatcctgg gacaaatcta cgccgggctt
      421 acgatggggc ggttgcgacc attcacaagg tgatctcgac cttgcaaaaa aaaccgcgtg
      481 gcatcgaacc gcgcgatttg agcccagcga gcggcaagcg caagttccgc tccaacttga
      541 ccccgcaagc atttcaagag gcggttaccc gggccaagga atatatcaaa gcgggcgaca
      601 tcatccagac ggtcctgtgc caaagactag aaacggaaac cgatagcgac ccgttcgaaa
      661 tctatcgcgc gctaagattc gttaacccgt cgccgtatat gtattacttg gaaatggagg
      721 acctgcgcgt catcggctca tcgcctgaaa ccatggtacg cctcaccggc gaaacgatcg
      781 agctgcgccc catcgcgggg acgcgcaggc gcggcactac accggaagag gagcgcgatc
      841 tggaatccga actgctggcc gatccgaaag aacgcgccga acatatcatg ctggtcgatc
      901 tcggtcgcaa tgatgtcggc cgggtggccg ccatcggcag cgtcgatgtc aacgagctca
      961 tggccatcga acgttattcg catgtcatcc atatcgtctc caacgtgcgc ggcaagctcg
     1021 cccccgacaa gactcctttt gatcttttcg tttcggcctt tcccgccggc accgtttccg
     1081 gcgcgccgaa aattcgcgcc atgcagatca tcgcggaatt ggaaccgcaa aaacgcagcc
     1141 tctatgccgg tgcgatcggt tactttggtt acaacggcaa tctcgatacg tgtatcgtca
     1201 tccgcaccat cgttatgaaa ggcaagaaag ttttcattaa cgccggtgcc ggcattgtcg
     1261 ccgactccga cccggtgctc gaatatcaag agacgttggc caaagcccgc gccatgttgc
     1321 gcgcggtgga gcttgctgag cagtggagga aacgatgatt ctgctcatcg ataactacga
     1381 ctccttcacg tacaacttgt atcattactt gggcgagctc ggcgccactg tccgtgtcgt
     1441 ctacaacgat aaaatcagtc tcgaggagat cgctgccctg cgtccggaaa agatcgtcat
     1501 ctcgccaggt ccgtgtacgc ccaaacaagc cggcatctct tgtgatgtaa tcaatcgttt
     1561 cggcgcgcag acgccaattt tgggtgtttg tttgggccat caagcgattg gcgccgccta
     1621 tggcgggcgg attatccgcg cgcctacagt catgcacggc aagttatccg cagtaagcca
     1681 cgacttcaaa accgtttatc gcggcttaaa aaatccgttc gcagccatgc gctatcattc
     1741 gttggtgatc gatcctgaga gcttgcccgt cgatctcctc gtcagcgctc gcaccgacga
     1801 cggcataatc atgggcgtgc gccacaaaac tcttcccgtc gaaggagtgc agtttcatcc
     1861 ggaatcgatt ctcgccgaag aaggcaaaaa gctcctgaag aattttctgg acagttatta
     1921 aaagatgaag ctgttgtgct tccaagccaa acggttccac tggaaaaccc attcgaaaac
     1981 gctgccggaa gcggccgatc agacaatcga ccaggaggtc agtgaatccg tcgtcgtttt
     2041 tctccatgcc gaagcggcgg acggcgacgg cgaaagaaac gtttccgtca tgcgccagac
     2101 tctcaagcat atcaagtggc tcgccaacaa gcgtgagctt aaaaccatcg tgctccattc
     2161 gttcactcat ctcggtggtg tcaccgcccc cgccgggttg gccgaaacac ttatcgtgac
     2221 attgcagcaa cgcctgagcg agaccggcta tcaggtctgg acgacaccgt tcggttactt
     2281 ttgcgaatgg gacctgagcg tttacggcga gagtcttgcc aaggtgtgga aagagattca
     2341 ctgatcgagt cactccgccg atccttaacc actgatctcg acgcaagttt tcccgccatg
     2401 ctatactcac gcagaatttg ggaggaaatc catgactgca aacacaaaac ttaattgctg
     2461 gataatagcg gcagcgatct tcttattagt cccctcgtgc aagaaaaccg accaggccgc
     2521 cagcccagac gatcttcccg atttaaataa gcaaatccgc ccttacgtca cgaccgataa
     2581 cgccaagatc cgcaccgggc ccgggccgca gttccgcggt atcgctgacg tgcccgccaa
     2641 cgccaaagtc catgtcgtcg gtcgcgatgg cgactgggcg ctgatcgtct cgaagaaagg
     2701 taacgcaccg ggttttattg aattggcagc gatcaagccg ggtgaaggtg agcccgaaaa
     2761 accggaattc ggcgaaccag ctaaagtcga gggtaaatac gaggccttgg ctgacacaca
     2821 ggtacgcagc ggccccggtc tgcattatcc atcggttgcg gatatcgcca agggcaccaa
     2881 actcaacgtc gtcgatgaac aaaaaggctg gctgaaagtc gaatccaagc gtggcaacaa
     2941 acccggctac gtcgaatcga gtctggccaa accggtaaac gagaagtagc gttcgcgcag
     3001 tcgagctata gcttcaacaa gcgcacggct gtgccaccga gcatgtcttc ccgatgtttc
     3061 tcgttcagcg gcaacttccg cacagctgcg acatagttgc gcaactcggt catccccttc
     3121 cccgtatcgg tattgacccc ggtgaagtct tgcggatagt cgctggcaaa gaccaagcgc
     3181 tccgggcgaa tgccttcgag ggcgcagcga agcgccgtcg tgccgccttc gaatccggcc
     3241 atgtcgaagt acaccatatc gaaatactcg gcgaatggtt tcttgagcgt accgaagcga
     3301 taacctttgc cgaccagccg gtctttcacc gcggcaattc cgccaccaaa gtgggccatg
     3361 acaattttta agttgggata acggtcgtag ataccaccgg cgatcaaacg cgtcgtcgcc
     3421 acggtcaagt cgacctcgcg ccccaggacg cgaggcaaat cgtaatcttg caaatgctca
     3481 tagccagtcg gcactaaggc cggatggacg aacaccggtg catcaagctc gcaggccaat
     3541 tcatacaagc catacagttt cggtgagtcg agcgaaaggc cgttcacctg cgaggtgatc
     3601 gtcaagccgc ggagccccaa ttcggtgatc gcccggcgaa gttcagtgca agcggcttcc
     3661 ccctctagaa ttggcgcctg agccaagccg acaaaatgcc ccggaaatct cttttgaatc
     3721 gctgccaaat cgtcgttaat gaagcggcac tcttccatcg gcgcggtcca gccaagcagg
     3781 caagacaata ctgatacatc cacgcccgcc tcgtccatat ccttgagttg caactccaag
     3841 tcgtaaagcc gctgatgcat ggtcgtcgcc ggcagaccgc cttcctgaag catagttcta
     3901 cctgtttccg aatagagtcc acgctttttg gccaactcga cgggaatata gtgatgctga
     3961 aaatcgacaa ccatttccga cgctccttcc tgaaattgtc taaataccta acacatccga
     4021 ttacgtccag aattagcagc ttggatgacc ttcaccactg acacaggtcg ggctggtagt
     4081 tcaactagat taaattttga tggagtactt ccgcccgaat tagtctgcgc gattcggatg
     4141 aatcatattc tgcccgggaa cgtatatttc gtgtagaaac accagttctg acttgtcagt
     4201 taaaggcaga tccctagaca agcccagtca acgtacccga tattccgagc ccgtgaaaac
     4261 aggaagcgca ggaaatttga tccggatgag ggaaccacgg tgcgatcatt acagagcatt
     4321 cagcgtcatg ccgggaagct tgaggacaaa cggcgctgcg ctgtggaccg gactgttaac
     4381 aattccatgg caccatcgtt gctcagagcc gagtgtgaag atgaacttca acgaaaaccc
     4441 aataggaatt ttatgtacaa aaagttttac ttaggtttga gcatcgggct cataagctac
     4501 atcgccactg gcccagcgat ggccaacagc ttggtgtctt tcgactttga cgggttgagt
     4561 agcaaagcat atcgctcaaa ggattacaca agcgtcgagt cgtacatgga aaatatctat
     4621 ggcgaggata tttccgttag caaaggaaca gcggcgttta aagggaattt cgcgccgctg
     4681 acgaattctt tcgttagtaa catcggtcag cccagcagcc ctggcatgta cttgcgcaac
     4741 agcgccaaca aacccatcgt gctcgatttc ggcaccaatg gaatcagctc attctcagtt
     4801 gactggaagc tattcaagaa cggcaaagga ctgaccatcc tggctgacgg catcatcgtc
     4861 gaccaacaga ccctcctaaa atctcaaaag aaaagtggtg ccggcggcaa ccttacactg
     4921 tatttcgacg atgcggtgca tcagctgcag tttgtcggtc ttaagaatac aaggttcggc
     4981 ctggacaacc tgctaattaa tttgccggag ccggacgccg gcggcttaga aactagttca
     5041 ctcacagaag aatccaacgg cagtaacgaa ggccaaggcg atcccggaga aagaattaat
     5101 cctttggaaa atatcgtccc gctcgtcgat acaacggcgt cggtgcccga accagcaagt
     5161 ctagtgcttt tggttgtggg tctatgcgcg gcgtggtggt cacgaagata tgccgtaagt
     5221 tcgtagtcac acccaactgg tttttcccgc cttgtaggac ttctcgtcgc aaagttcacg
     5281 tccaggtgcg ctgttaattt tcctggcatc cattactgca gattccgcgc tcacgattta
     5341 aaaggccctg cacgattcga tccgtgactc tggcagacac caacaattag cgcgatcaaa
     5401 ccgagcggca ccaaccaggt gaaggcaaat tgctgcgccc ccgcgagtgc ttgccgtgac
     5461 gaccagagct taggtccgcc atcgatctcc atcagcgcga cagcaatggt cgtgcctatc
     5521 gtcccggcgg ctgtgccgaa cgaactcagc atcgccgcca gcacgccata gtcattctgg
     5581 gttaccgacc caaaggcgac tttctgattc gccggagtga aaactccaat gcccgcgcca
     5641 agaagcccca acacagcacc gacccatgca aaatgcgaat cgacgcctaa gcatgcgtaa
     5701 ccggcaatac cgacgacgat caagcctatc ccaaccgcag acaccgacgt ggcatcgaag
     5761 cgatcggcta accggccgct cagcggcgaa agtaaggcgg tggacaatgg caagatcgcc
     5821 agcaggaaac ccgcgccaag cgcactcgcg cccaagacat tttgtaagta gagcggcgcg
     5881 gccaaggcga tcgggttgtg aatcatgtga ctcagaataa ctgcgatcat ggcggcggac
     5941 aatgccttgt tgcggaaaat agtcacgtcg accaaaggcc gttgtgtgtg acgttcaatt
     6001 gcgacgaagc tggcaagtcc ggcagcggcc acagcgaacc aagaaataac ccacggtgaa
     6061 ttccaaccga gggcggcgcc gaagcttagg ccgatcagta acgacggata accaatcagc
     6121 aacgctaccg aaccccatat atcaaattgc tcgtggcgct gttcaccggc gccgcgaaat
     6181 atttttatca ccgcgacagt gacggcagag ccgacgacga aattcatcca gaagatccag
     6241 gtccagccac ccaattgcag caaaaaaccg ccgacgctcg gtccggtcat gcgtcccacc
     6301 gcgatggccg aagcaaaggt gccaagcact tgaccacgcc gctcgttggc gaaactactg
     6361 gctaacaacg ccgtcgacat cggcaatgcc atcgccaacc caaccgccat cacggctcgc
     6421 gccattaaaa gctgcgaaaa cgacaccgcc agcgcgccca ggcccgcacc cgtggcgtaa
     6481 agcgccacgc cgatcttgta aagccggcgc tggccgagaa tggcgccggc gcggccgaaa
     6541 caaaacgaca aactggagat cgtcactaca cccatgagcg tgatccactg gatctccgac
     6601 aaactattgc cgaactgctt ttggatggtc ggcaaggcaa cgctgatgct gccgaagctg
     6661 ttgaagaaca aaaatgccgc caagcaggct accgagagga ttgcccgttc ttcagcgatc
     6721 gataaagccg cgcccggcgc agtcgtttga tgtggcgata tcatggaaga acattttgac
     6781 gtagttttct tggtctatgt caagctttag ctccagcgaa ctgtgaaata gggcgtgctt
     6841 ttcctgcaaa tgccaccagc gcttggctta atcgaagcga caccggctta cagttttctc
     6901 agccattcac caatttgtgc ggctctgttg agctgaaccg cgccgagcag tgtccggcgt
     6961 agggttaaaa tatctctaga actcccttgc ttttcggtct gcaccagcct ttctccatct
     7021 ggcatgggtt tagcgttggt aaattatcaa acc
//
LOCUS       DATLCH010000159         6831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4916487, whole genome shotgun sequence.
ACCESSION   DATLCH010000159 DATLCH010000000
VERSION     DATLCH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4916487"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1117)
                     /gene="rodA"
                     /locus_tag="VMZ02_04615"
     CDS             complement(14..1117)
                     /gene="rodA"
                     /locus_tag="VMZ02_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HUR71245.1"
                     /translation="MQIDRRLISHFDWTLFLLVLSFVAIGITTIYSANYNLTTGHAGA
                     LPMRQFTWLGLGLIAMFVAISFDYHYIDRLAYPFYGLMLLLLILVIIVGHSGGGSQRW
                     INLGFFRLQPSEPAKLAIVLVMAKYFQSDEPAKGYYLRDLWVPFVIVAPLILLTLIQP
                     DLGTAIILGIVFVSMVLMGGLRLRSFSYLVAAGIALLPIGWQFLKTYQRNRILTFLDP
                     DRDPLGAGYHVIQSQIAVGSGKLFGKGWLHGTQNRLNFLPEQHTDFIFAVFSEEWGFV
                     GCAVLLLMFVMMIAYCLRVVQRAKDRFGALLVFGMLSIVFWHVTINVSMVTGLMPVVG
                     VPLPMVSYGGSALASMMFAVGVIINVSMRRYVF"
     gene            complement(1107..2984)
                     /gene="mrdA"
                     /locus_tag="VMZ02_04620"
     CDS             complement(1107..2984)
                     /gene="mrdA"
                     /locus_tag="VMZ02_04620"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HUR71246.1"
                     /translation="MAALQINKQDGAPEIRRRANYLYVLLAVVFIGLTARLVHLQWLH
                     GERYTFLSENNRVRLKRVPGTRGMIFDRRGELLVDSRPSFDLIFVPEDAKEPEQTLRE
                     LAGRLAWSADELLQTYAENKSRSAFDEIVLGRDIEWPAVVAVEAHQLDLPGVSLRVRP
                     RRNYADGPIAAHILGYMGEINQRQLKNYKEQGYLVGDEIGQSGLERHWEDVLRGQHGG
                     QQVEVDALGRRVRVLHQVNDVPGYTVHLTLDRQMQQTAYEALQGKEGTIVAIDVNSGA
                     ILALASTPAFDPNVFARGIKGKEWNALVNDRLRPLSNRATQGQFPPGSTFKVVMFIAG
                     LEEGAVDPSAQISDPGFYHFGNRNFRDWKQGGHGAVNLHRSLVESCDTYYYQLGAKLG
                     VDKIAKWARAFGLGEKTGIALDDERNGMIPDSAWKLKRYRQPWFPGETLSVAIGQGYV
                     TVTPLQLANMMAAVANGGKLYRPRLVSKVESVDGSSVREFGPELIRTIEMKPDTLSRV
                     HNALADVVKAGTGGMAKSALVEVAGKTGTAQVVEMKGGYVKSEQLSRFTRDHAWFVSY
                     APVQSPQVAIAVLVEHGGHGGDAAAPMAKKVYEKFIELQKQPSENQHVKAPAPGETLA
                     D"
     gene            complement(2997..3503)
                     /gene="mreD"
                     /locus_tag="VMZ02_04625"
     CDS             complement(2997..3503)
                     /gene="mreD"
                     /locus_tag="VMZ02_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HUR71247.1"
                     /translation="MKLFLLFFLAAIFLIVLQTTFFHLLPMGPVVPDLILVLCVYLGL
                     YHPSVGAAFGAFLLGYSIDIVSSPILGFNAFAMSLVYLAVYLCARSIWLHHPIVSSVV
                     VLLAALVKGATMVLIWAIFLSVDGFWAGAARYVMWEALFAALLAPLVFALLRRGQHLV
                     DRFTPVTP"
     gene            complement(3535..4368)
                     /gene="mreC"
                     /locus_tag="VMZ02_04630"
     CDS             complement(3535..4368)
                     /gene="mreC"
                     /locus_tag="VMZ02_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HUR71248.1"
                     /translation="MLAFLRRNQIFFSSCFCLLLSLYILTAAARGQIKNEPVGTLLMW
                     ILRPLQIAGQGSANWIKGFRNHYDTLAGFRSENERLRKRLQTLEIERQRLLEAEATNR
                     HLQQLLDFRSELPSGAITATIIASSASSWFQSCVISKGSADGVRKGMAVVTPLGVVGK
                     VVAVTGRTAKVLLLTDPNSGIDVLVQRTRSRGIVSGSLESGTVLKYIKRSEDVQEGDR
                     LVTSGLDSGFPKGLLVGTVIKVRKQNRGLFQSVEVWPAVQSARVEEVLVVGAQPDVAK
                     K"
     gene            complement(4413..5447)
                     /locus_tag="VMZ02_04635"
     CDS             complement(4413..5447)
                     /locus_tag="VMZ02_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HUR71249.1"
                     /translation="MFDALFGVFSNDLAIDLGTANTLIYVKNQGIVCNEPSVVAVQQR
                     NERGGKKVLAVGAEAKRMLGRTPGSIVAIRPLKDGVIADFEITEAMLRYFIQKVHNRR
                     TLVRPRIIVGVPFGITEVEKRAVRESAESAGAREVYLIEEPMAAAIGAGLPITEPTGN
                     MIVDIGGGTTEVAVISLSGIVFSRSVRVGGDKMDEAIAQFIKRKYNLLVGERTAELIK
                     ITIGSAYPGDEVQTMEIKGRDLVAGVPKTVVITDEEIRDSLLEPINQIVEAVRLSLER
                     TPPELASDIVDRGIVLAGGGSLLRNLDILLREETGLPVMMADDPLTAVVMGAGKALDE
                     ITLLREVAVH"
     gene            5644..>6831
                     /locus_tag="VMZ02_04640"
     CDS             5644..>6831
                     /locus_tag="VMZ02_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HUR71250.1"
                     /translation="MLDFLRKRKRNWAILFLLVLIIIVFVAFYGGNKMGDRATSEVAM
                     INGETISQREFAVEYQRALERYREMLKGQLTEEMIKGLNLKGNIVEGLVQKKLVLQEA
                     RSLGLLATDDDVANQLSKVPEFQVAGRFNKDRYLQILQANRLQPAQFEEDQRNQITLQ
                     RLYSIILDSIQVSDAEVRERYRLDQEKINLQYVKLSVNDFVGQVKLTDEDIKKFYERN
                     KETFKEPLKIQVEYLTYPFEQFSGGAQVSAKEIEEFYQNNQKTKFHRPREAKIRYIAL
                     AIAPTASAEEKKAIMAKAEGIVKEARRGKDFAQLAKQQSSDSTAAKGGDVGWVIQGQM
                     PPAIDKLIFGIGKGDVSDPVETPGGVQIFKIDDVRDEKNLTLTEATPEITQMLKTEQA
                     KKEA"
BASE COUNT         1739 a         1918 c         1771 g         1403 t
ORIGIN      
        1 cgccaatcca tcttcagaaa acataccgcc gcatactgac attgatgata acgccgacgg
       61 cgaacatcat cgacgccaga gcggagccgc catagcttac catcggcagc ggcacgccaa
      121 cgaccggcat aagtcccgtc accatggaaa cattgatcgt cacgtgccag aacacgatcg
      181 acaacatacc gaaaaccaag agtgcgccga agcgatcttt ggcccgctgg acgacgcgta
      241 gacaataagc aatcatcatt acgaacatca acaagagcac ggcgcaaccg acgaagcccc
      301 attcttccga gaacaccgcg aaaataaaat ccgtatgttg ctccggtaga aaattcaagc
      361 ggttttgcgt gccatgcaac cagcccttgc caaatagctt tcccgaaccc acggctattt
      421 gcgattgaat gacatggtaa cccgccccca acgggtcgcg gtccgggtct agaaaagtca
      481 gaatgcggtt gcgttggtag gtctttaaaa attgccaacc aattggcaac aaggcgatac
      541 cggcggcgac cagataagaa aacgaccgta gcctcaaacc gcccatcagt accatactaa
      601 cgaaaacgat gccgagaatg atcgccgtgc ccaaatcggg ttgaatcaaa gtcaataaga
      661 ttagcggtgc aacgatcacg aagggcaccc agagatcgcg cagatagtat cctttggccg
      721 gttcgtcgct ctgaaaatat ttggccatga ccaaaacgat ggcgagtttg gcgggctccg
      781 acggttgcag ccgaaaaaag ccgagattga tccagcgctg tgaaccgccg ccggaatgcc
      841 cgacgattat taccagaatc aaaagcagca gcatcaatcc atagaatgga taagccagtc
      901 gatcgatgta gtgataatcg aagctaatcg cgacgaacat tgcgatcagg ccgagtccga
      961 gccaggtgaa ctggcgcatc ggcaaagcgc ccgcatggcc ggtcgtgaga ttgtaattgg
     1021 cgctatagat ggtggtgatg ccgatcgcga cgaaagataa cacgagtaga aaaagcgtcc
     1081 agtcgaagtg cgagatcaaa cgccggtcaa tctgcaaggg tttctcccgg cgccggcgcc
     1141 ttcacatgct gattttcgct cggctgtttt tgcagttcga taaatttttc gtaaaccttc
     1201 ttggccatgg gtgccgcggc atcaccgcca tggccgccgt gctcgaccag cactgcaatg
     1261 gcgacctgcg gcgactggac cggagcatag gaaacaaacc aggcgtggtc acgcgtgaag
     1321 cggctcagct gttcagattt aacgtagcct cccttcatct caacgacttg ggcggtgccg
     1381 gttttgcctg cgacttcgac gagcgccgac ttcgccatgc ctccagtgcc ggctttgacg
     1441 acatcggcaa gggcgttgtg cacccggcta agggtatccg gtttcatctc aatcgtacgg
     1501 atcaattcag gaccaaactc gcgcacgcta gagccgtcca ccgattcgac cttgctgact
     1561 aggcggggcc gatacagctt gccgccgttg gccacggccg ccatcatatt agcgagttgc
     1621 aatggcgtta ccgttacata accttggccg atggcgaccg atagggtttc gccgggaaac
     1681 cacggctggc gatagcgttt caatttccag gcagaatcag gaatcatgcc gttgcgttca
     1741 tcgtccaaag cgatgccggt tttttcaccg agaccaaagg cgcgcgccca cttggcgatt
     1801 ttgtcgaccc ccaacttggc gcccaattgg tagtaatagg tgtcacatga ctcgaccaag
     1861 gaccgatgca aattcaccgc gccatgaccg ccctgtttcc agtcgcgaaa attacggttg
     1921 ccgaaatgat agaaacccgg atcggagatt tgcgcactag gatcgaccgc gccctcttcg
     1981 agtccggcaa tgaacatgac taccttgaaa gtcgagcccg gcgggaactg tccctgcgtg
     2041 gcgcgattgc ttaacggccg cagccgatcg ttcaccaagg cgttccattc tttgccttta
     2101 atgccgcgag cgaatacgtt cggatcgaat gccggcgtgc tggcgagcgc caagattgct
     2161 ccagagttca catctatcgc gacgatcgtc ccttctttgc cctggagcgc ttcgtaggcg
     2221 gtctgctgca tctgacgatc gagtgtcagg tgcacagtgt aaccgggaac atcgttgact
     2281 tggtgcagca cgcgcacccg gcgtccgagc gcgtcgactt cgacctgctg gccgccatgc
     2341 tggccacgta aaacatcttc ccagtgccgt tcgagaccgg attgaccgat ctcatcgccg
     2401 actaaatatc cctgctcttt gtagttcttc aactgacgct ggttaatttc acccatgtag
     2461 ccgaggatgt gagcagcgat gggaccgtcg gcgtagttgc gtcgtggccg cacccgcaag
     2521 ctcacgccgg gcaagtcaag ttggtgcgct tccacggcaa ccaccgcagg ccactcgata
     2581 tctcggccca aaacaatttc atcgaacgcg gagcgactct tgttttctgc atacgtctgc
     2641 aagagttcgt cggcactcca cgccaggcgt ccggcgagtt cgcgcaacgt ctgctcaggt
     2701 tctttggcgt cttccgggac aaaaataaga tcgaatgaag ggcggctatc gaccagcaac
     2761 tcgccgcggc ggtcaaagat catgccgcgg gtaccgggca cgcgcttaag ccgcactcga
     2821 ttgttctctg acagaaacgt gtaacgttcg ccgtgcagcc attgcaaatg gaccaggcgc
     2881 gcggttagac cgatgaagac cacggcgagc agcacataaa ggtaattcgc gcgacggcga
     2941 atttccggcg caccatcttg tttgttgatt tgtagtgccg ccatcgtctt attttattat
     3001 ggagtcaccg gagtgaatct atccactaaa tgttgaccgc gccgcaatag tgcgaaaacc
     3061 aaaggtgcga gcagcgccgc gaacaaagct tcccacatga catagcgcgc cgctcccgcc
     3121 caaaaaccat cgacactcaa aaatatcgcc caaatcaaca ccatggtagc gcctttgacc
     3181 agagctgcca ataataccac gaccgaactg acaatcggat gatgcaacca aatcgagcgc
     3241 gcgcacaaat ataccgccaa atacaccagc gacatggcaa aggcgttaaa gcccaagatc
     3301 ggactcgaga cgatatcgat ggaatagccc aaaagaaacg cgccgaatgc cgcaccgacg
     3361 gacggatgat agagacctaa gtaaacgcac aagacaagga tcaagtcagg caccaccggc
     3421 cccatcggca ataaatgaaa aaatgtcgtc tgcagaacga taagaaatat ggccgctaaa
     3481 aaaaacaaca ggaacagttt catgggcgca ctatttggaa cggcggatcg gcggctactt
     3541 tttagctacg tcgggttgcg cgccgaccac cagtacttcc tcgacgcgcg cagattgtac
     3601 agccggccac acttcaacgc tctgaaacag tccgcggttt tgtttgcgca ctttaataac
     3661 ggtaccgacg aggaggccct tgggaaaacc actgtccaaa ccagaggtca ccaaccgatc
     3721 accctcctgc acgtcctcgc tccgtttgat gtattttagc acggtgccgc tttcgagcga
     3781 accggaaacg atgccgcggc tgcgcgtgcg ttggactaag acgtcaatgc cgctgttcgg
     3841 atcagtaagc aaaagaactt tagccgtccg cccggtgact gcgacgactt tgccaacgac
     3901 gcccaacggt gtcacgaccg ccattccttt gcgcacgcca tccgcgctac ccttactaat
     3961 gacgcaactt tgaaaccagc tgctcgcact gctggcgatg atcgtggcgg taatcgcacc
     4021 gctcggcaac tcgctgcgaa aatcaagtaa ttgctgcaag tgacggttgg tcgcttcagc
     4081 ttccagcaat cgctgccgtt cgatttcgag ggtctggagt cgctttctca ggcgctcgtt
     4141 ttcgctgcgg aagccggcga gggtgtcata atgattacga aatcctttga tccagttggc
     4201 gcttccctgt ccagcgattt gcagcggccg gagaatccac atcaacagcg tgcctacagg
     4261 ttcgttcttg atctggccgc gcgccgccgc cgttaagata taaagcgaaa gaagaagaca
     4321 gaaacaggag ctgaaaaaaa tttggttcct gcgtaggaaa gcaagcatgg caggctcgat
     4381 cggcgcctaa atacggaatc catggggcct atctagtgaa ccgcaacctc ccgcagtagc
     4441 gtgatctcat ccaaggcctt gccggcaccc atcaccaccg ctgtcagtgg atcgtcggcc
     4501 atcatcaccg gcagtccggt ttcttcgcgc aggagaatgt ccaagttacg caacaaagag
     4561 ccgcccccgg cgagtacgat accgcgatcg acgatatctg aagcgagttc tggaggtgta
     4621 cgttctagag acaagcgcac cgcttcgacg atttggttaa tgggctcgag caacgaatcg
     4681 cgaatttcct catcggtaat gaccaccgtt ttcggcacgc cggcgaccaa atcacgacct
     4741 ttgatttcca tggtctgcac ttcatcgccc ggatacgccg acccgatggt gatcttgatg
     4801 agctcggcgg tgcgctcacc gaccagcaga ttgtatttgc gtttgatgaa ctgagcaatt
     4861 gcttcgtcca ttttatcgcc accgactcgc accgatcgcg aaaaaacgat accggaaaga
     4921 gagatcaccg cgacctctgt cgtcccgccg ccgatatcga cgatcatatt acccgtgggt
     4981 tcggttatcg gcaggccggc gccgatcgcc gcggccatcg gttcctcaat cagataaact
     5041 tcccgcgcgc cggcggactc cgccgattca cgtacagcgc gtttctccac ttcggtgatg
     5101 ccaaagggca caccgactat aatacgcggg cgcactaaag tgcgccgatt gtgaaccttt
     5161 tgaataaaat accgcagcat ggcctcggta atttcgaaat ccgcgatgac gccatcctta
     5221 agcggtcgaa tcgcgacgat gctgccggga gtacgcccca acattcgttt tgcttccgca
     5281 cccaccgcga ggactttctt accgccgcgt tcgttgcgtt gttgaacagc aacgactgag
     5341 ggctcgttac acacgatgcc ttggtttttc acataaatga gtgtgttggc ggttccaaga
     5401 tcaatggcta aatcattgga gaagacgcca aagagggcat cgaacatcag taactccttc
     5461 tcatgcgtgg cgaaacggac ttaagttagg agaaaatatc agattttccg gaaactggca
     5521 aggtaatgat aaatcaacgg aaaattcatt ttgatggaac tcccgcggtt gcatatctga
     5581 aggccatttt ttatcatagt tgggctagtt tttacgttgt tttcacgaaa ggttctatca
     5641 cgaatgctcg atttcctacg caaaagaaaa agaaactggg cgattctctt cctgcttgtg
     5701 cttatcatta tcgtctttgt cgcgttttac ggcggcaaca aaatgggcga ccgcgccact
     5761 tccgaagtcg cgatgatcaa tggggaaacg atcagccagc gcgaattcgc agttgaatat
     5821 caacgcgcac tcgaacgcta tcgcgagatg ctcaagggcc agctcaccga agaaatgatc
     5881 aaaggactca atctgaaggg aaacatcgtc gaaggtctgg tacaaaagaa gctggtcttg
     5941 caggaggcgc gtagtctcgg gctgttagcc accgacgacg atgttgctaa tcagttgtcc
     6001 aaggtgccgg agtttcaagt cgctgggcgc tttaataaag atcgttactt acaaattttg
     6061 caagccaacc gcctgcaacc ggcgcaattc gaagaagacc agcgcaacca gatcacgctg
     6121 caacggctct atagcatcat tctcgactcg attcaggtga gcgacgccga ggtgcgcgaa
     6181 cgctatcggc tcgatcaaga aaaaatcaac ctgcagtacg tcaaactgtc ggttaacgac
     6241 ttcgttggcc aagtcaaact caccgacgag gacatcaaaa agttctatga gcgcaacaaa
     6301 gaaacgttca aagagccact caagatccaa gtcgaatatt tgacctatcc tttcgagcag
     6361 ttctccggcg gtgcccaagt gagtgccaaa gagatcgaag agttttatca aaacaatcag
     6421 aaaacaaaat ttcaccggcc ccgcgaagca aagattcgct atatcgcgct cgccattgct
     6481 cctaccgcca gcgctgaaga aaaaaaagcg ataatggcaa aagctgaagg gatcgtcaaa
     6541 gaagcgcgcc gcggcaaaga ctttgcccaa ctggccaagc aacagtcgtc cgattcaacg
     6601 gccgccaagg gaggcgatgt cggttgggtt atccagggcc aaatgccgcc ggccatcgat
     6661 aaattgatct tcggtatagg caagggtgat gtcagcgatc cggtggagac ccccggcggc
     6721 gtgcaaatat tcaagattga cgacgtgcgc gatgaaaaaa atctaacgtt gacggaggcc
     6781 acgcccgaga tcacgcagat gctcaagacc gaacaggcaa aaaaagaagc c
//
LOCUS       DATLCH010000160        10472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_449915, whole genome shotgun sequence.
ACCESSION   DATLCH010000160 DATLCH010000000
VERSION     DATLCH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449915"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VMZ02_04645"
     CDS             complement(<1..869)
                     /locus_tag="VMZ02_04645"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR71251.1"
                     /translation="MIQTKGLTGAQSMLRVLGKMGVEKIFASPGSEWSPLWEALAEPG
                     VEGMPQYYSTRHEEIAVGMASGYAKSTGKLPAVVIHTTVGALHATMAMRAALHEQVPM
                     VVFAGESIGFGEDEGPDVGGQWQGHLGDMGGPAKLVDHTVKWSFGVNTKSIVPSTVQR
                     ACRLAMAGPRGPVFVSMPMEYMFDTMTKDVPSDLGSSPSATADPKGIEELANLLAGAQ
                     NPVIVAEDAGKTTKSVNLLVEIAELLGCSVVESRSTGVINIPRTHPLHSGYDVKEAVQ
                     GADVIFLAAVIAP"
     gene            complement(1011..2027)
                     /locus_tag="VMZ02_04650"
     CDS             complement(1011..2027)
                     /locus_tag="VMZ02_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71252.1"
                     /translation="MELREAIKVLDSDAHARDLDNDIRQYLPEPYRNQRSSFIPSEHY
                     DRTLGGTLGRSGAKVEERLEAMDTQQIDTAVMFPTSGLGVGRVRDSKFQVALCRAYNN
                     FISDYCKASPRLKAVANLPVNDPAECTNELNRAVTKLGLCGGMLVAQAHRKNLGSPEY
                     YPLYEEAQRLNTPISIHAFGGDEPGSEIFDQFISLHTCGHPFPVLRQLTAMVFGGIPE
                     MFPKLRIGYLEVGCGWLPYWAERMDEEWEKRGKVEAPLCKKKPSEYLLSENSYYGCEP
                     EEKMIGYVTKEIGSKTLMYASDYPHWDMSWPESATILWNRNDISLEVKKDLLLNNAKR
                     FYNI"
     gene            complement(2270..3412)
                     /locus_tag="VMZ02_04655"
     CDS             complement(2270..3412)
                     /locus_tag="VMZ02_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71253.1"
                     /translation="MEQSIVSSDSHVIEVPDLWEKGLPAAMRARAPKMFFDEKRDAWM
                     FGSPEVQTQAVGGLFMAGQQPENVEKFRKAGFAVARPGGWDPLERQADMMTDGVAAEV
                     LYPSLGLGLYCVEDAALQEGLFRTYNDWIIDYCQKVPDRLYGVALISMYNVDHAIAEM
                     ERCKKQGVVGTMIWQVPHPSLPFTSEHYEKFWAASQDLDLPVHLHILTGFGDSMHRQT
                     AHGPKRYRIGLEQTREIENALFELIFHGVLERHPKLKIVSVENEVGWMPFWLGQADKG
                     YKRHRHSEKVQMSKLPSEYFAEQIYATFFNDVVGGKMFSSWGVDNCMWSNDYPHQNST
                     WPNSRAVIERDMAHLPAESREKLLNTNVRKLYKLNAPVSLPKAVAA"
     gene            complement(3428..4417)
                     /locus_tag="VMZ02_04660"
     CDS             complement(3428..4417)
                     /locus_tag="VMZ02_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71254.1"
                     /translation="MRRALLIVVLGMLATLPADCRAAEKFTTIYSARVMAQALPWIAE
                     EAGLFKKYNLDHSLVFIASSSIVTAALLGGDPDMTMTGGVGNVIAYVRGSTEIAYVGS
                     AKNLMTQNIVAGGNLKRPEDLKGKRIAVSRIGSNSHYFTIQALRRHNMEPNRDYTFLQ
                     SGGDPESLIALMSGGVEVACLTPPTDVQALTRGYHYVVYGPDLKIPYAATSFVTKRST
                     IARRPQAMAQYLRAMGEASKIMHSDREFVYKVLGKQLRINDRAIMEAAYNAEIKVMEP
                     RLALKAEAFQAILDEVAQTDERAKKVKPQELTDARFLNDMEKSGFWDQIWAKR"
     gene            complement(4417..5415)
                     /locus_tag="VMZ02_04665"
     CDS             complement(4417..5415)
                     /locus_tag="VMZ02_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71255.1"
                     /translation="MVNVSRLAALFLVLVSAGQTIAAGAPDKLVGIHSSRVMSQSFPW
                     VAQEAGIFKKYNLDFNLVFISSSSIVTAALLGGDAEMTVTGGIGNVAAYVKGSTDVVF
                     TGGVKNVMTQTLVAGGNIKKPEDLKGKRIGVSRIGGNSHYFTIQALRRHNMEPNRDYS
                     FLQTGGDPETLAALMSGSLEVANLTPPTDAMAIAKGYHYVVYGPDLKIPYSATAFVTK
                     RSVIAKRPAVIGNFMRAMAETSKLVHTDREFFYKVLGKYLRIQDRSVLDSAYNAEIKA
                     LEPRLTIKLESLQAILDELAPTDARAKTIKPADMVDSRYLDEMNKSGFMDQLWTKK"
     gene            complement(5737..6849)
                     /locus_tag="VMZ02_04670"
     CDS             complement(5737..6849)
                     /locus_tag="VMZ02_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71256.1"
                     /translation="MAAVKCSADSLRRGGGRSVRYGKTFSRRSLRLRRECLGNGDRIA
                     AGIFFLSAILIMPFVASTAYGASANTIRIGYPQPSGAMLPIWVMAETKLDQKYGVDLQ
                     NIYISGGARLTQTLVSGDIELAATGGAVINAVLSGADLTYIAASVPTYGFSLYVRNDV
                     KDVPSLKGKVVGLMTKGASTDHGLTALLRQYNLRSGQDVKVLYLGGVREGLAALDRGI
                     VSATTISAPTTLMARRMGYKELVNFMNLKIPYIQLGIVAHRAFLRQQPERIKAFLRGY
                     VAAIKIVNEDSDISKRALAKYLATGDSAILDEAYQAYRGVYPKVPYTTDDQVRSILAV
                     ADHPRAAGADPKDFFDNRWLKELEDSGFIAELYGGR"
     gene            complement(6836..7828)
                     /locus_tag="VMZ02_04675"
     CDS             complement(6836..7828)
                     /locus_tag="VMZ02_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUR71257.1"
                     /translation="MKLSSCLKLLLAVIALIIGAEAKLYSASATIRVGYPQPSGAMLP
                     LWLVAEAKLDQKYGVPVQNIFISGAARMNQAMVAGDIDLASIGGAVVNTVLGGGDLVA
                     IAVGVPTYGFSFYARPEVKDIASLQGKVIGIMSKGASSEHAVIALLRRYNLQPGKDVK
                     FIYLGGVREVLAAIEHSIAAAGVLSSPTTLAARRMGQKELVNIASLGLPYMHSGVATR
                     RAFAREQPERIKAYLRAYMAAIKIANEDAETSKRALSRYLVTTDSAILEEAYQSFRGI
                     FPRVPYVTEDQIKSSLALSEHPKAAAANPKDFFDTRFLKEIEDSGFVQELYGRR"
     gene            complement(7857..8882)
                     /locus_tag="VMZ02_04680"
     CDS             complement(7857..8882)
                     /locus_tag="VMZ02_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71258.1"
                     /translation="MAAKRKVCLTGPMRIKIVENTLREAGCELVLGKPQEDFRNFRYE
                     RKELINLVGDSQIIYPSGRDYIGGDVMDSCPELQAVVKSSIGIETVDVDAATDLGILC
                     CNSPTPENYLGVAEATMGLMVALFKRLKLNEAVIRNGGWKDLENRGTLMLGRTVGIIG
                     VGRIGQAVAKRLDAWGMKVLGYDPYVKQETVASLGIRMVGLDELLKSSDAVTLHVVLT
                     RETRGMIGMRELTMMKPTAFIINTSRGPAIKEADLIRALNGNLIAGAALDVFEQEPLP
                     KDGPLSKVDPFKLILTPHNIGANPGSGEAGQTRAAQSILAILEGKVPDTVVNPDAIAL
                     WKQRFWS"
     gene            complement(8894..9913)
                     /locus_tag="VMZ02_04685"
     CDS             complement(8894..9913)
                     /locus_tag="VMZ02_04685"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71259.1"
                     /translation="MAGQFEVIDADGHITESDAQLKKYMPEKYVKRVSSLTPGDSWDR
                     SLSGKLGSRAGDARSWLEAMDRGSVSTAVLYPTNGLSVGWIREPDVAVQYAQAWNNFC
                     SEEFQKVSPRLKCVALVSFQDVSEAVKELRRAVNELKLVGLMLPAVGLRLPLGHESFW
                     PIYQEAEKLDCMVGVHATVRGPHYFAGDLFDQFIEVHTLSHSFAQMMHCTSLMFRGVF
                     DRFPKLRVAFMEAGCSWAPYWFGRMSEEWEIRGEVEAPMCKKKPIEYLKEGRIYFHAE
                     DYEPLIGATTGILSHRNIYYASDFPHWDNEFPDNIAHLNKREDLNEEAKRWLFAESAK
                     KLYNL"
     gene            10293..>10472
                     /locus_tag="VMZ02_04690"
     CDS             10293..>10472
                     /locus_tag="VMZ02_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71260.1"
                     /translation="MKRTCYLTLLITWTLWTRTISQTSDTWSAAPGLASQDKCLASAK
                     DKLDMWKQFKDAKFEK"
BASE COUNT         2269 a         3076 c         2773 g         2354 t
ORIGIN      
        1 cagggagcaa tgacggctgc cagaaaaatc acatcggcgc cctggaccgc ttcttttaca
       61 tcatagccgc tgtgcaaggg atgtgtgcgc ggaatattga ttactccggt gctgcggctt
      121 tcgaccaccg agcagccgag cagttcggca atttctacca gcaggttgac gctcttggtc
      181 gtcttgccgg catcttcggc gacgatgacc ggattctgtg cgccggcgag caggttggcg
      241 agctcctcga tgccctttgg atcggcggtc gccgacggcg aactgccaag atccgacggc
      301 acatccttag tcatggtgtc gaacatatat tccatcggca tggacacaaa caccggtccg
      361 cgcggtccag ccatcgccag gcggcaggcg cgctgcaccg tggacggcac gatcgacttg
      421 gtattgacac cgaagctcca ttttaccgtg tgatcgacca attttgctgg cccgcccatg
      481 tcgcccaaat gcccctgcca ttggccgccg acatccggtc cttcgtcttc gccgaagcca
      541 atagactccc cggcgaatac gaccatcggc acctgctcgt gcaacgccgc acgcatggcc
      601 atggttgcgt ggagagcgcc caccgtggtg tgaatcacca cagccggcag tttgcccgtg
      661 gatttcgcgt agccgctggc catgccgacg gcgatctctt cgtggcgagt gctgtagtat
      721 tgcggcatcc cttcgacgcc cggctcggcc agcgcttccc acaggggcga ccactcagat
      781 cccggcgatg cgaagatctt ttcgacgccc attttcccca ggacgcgcag catcgactgc
      841 gcaccggtca atcctttagt ttgaatcatg cttcactcct ctcatgtcgt tgttaagaac
      901 gcggtatgcg aaatgcgcgt tttctatgtc gtcctcggcc acggaagcaa cgaataatct
      961 gaccggcgcg ctgggcggcc aatcacgatt cctcgctttt agagaattag ttaaatgtta
     1021 taaaaccgct tggcgttgtt gagcaaaaga tcttttttta cttcgaggga aatatcgttg
     1081 cgattccaca gaatcgtcgc cgactccggc cagctcatgt cccagtgcgg gtaatccgaa
     1141 gcatacatga gagtcttcga accgatctct ttggtcacgt agccgatcat cttctcttcc
     1201 ggttcgcagc cataatagct attttcactc aagagatatt cactgggctt tttcttgcac
     1261 agcggcgcct cgaccttgcc gcgcttttcc cactcttcgt ccatgcgctc ggcccaatag
     1321 ggtaaccagc cgcaacccac ctcgagataa ccgatacgca gcttgggaaa catctccggg
     1381 attccaccaa acaccatcgc tgtgagctgc cggagaaccg gaaacggatg accgcaagta
     1441 tgcaagctga taaactgatc gaagatctcg ctaccgggct catcgccgcc gaaggcatga
     1501 atcgagatcg gcgtattgag acgctgcgcc tcttcgtaga gcggatagta ttccgggctg
     1561 ccgagatttt tgcggtgcgc ttgggcgacc aacatgccgc cgcacaatcc cagcttcgtg
     1621 accgcgcggt tcaactcgtt ggtgcactcg gcgggatcat tcaccggcaa atttgccaca
     1681 gctttcaagc gcggcgatgc tttgcaataa tccgaaatga aattattgta cgcacggcag
     1741 agcgccactt ggaacttgct atcgcgcacc cggccgacac ccaggccgct ggtgggaaac
     1801 atcaccgcgg tgtcgatctg ctgggtatcc atcgcttcca accgctcttc taccttggct
     1861 ccggaacggc ccaacgtccc gcccaaagtc cggtcatagt gctcgcttgg aatgaacgag
     1921 gaacgttggt tgcgatacgg ctccggcaag tattgcctga tgtcgttgtc gagatcccgc
     1981 gcatgagcgt ccgagtcgag aacttttatc gcttcacgta attccatgat ccgctcctga
     2041 gcaaaacgac cctggctttg agtcgaaggg ttctaaattt atcgactgga agtcgaattt
     2101 tcattgcgtc attccggcgc aggccggaat ccaggttttc attggccttg cagtgctggt
     2161 gaagataaat gccggccttc gccggcatga caaatcttca cttcgcctga aggcgagaga
     2221 tttagccatc cccgagatgg acattaattt tccggctcgg gaccacgctc tacgccgcca
     2281 ccgccttggg caaggacacc ggcgcgttca gtttataaag tttgcgcacg ttagtattga
     2341 gcaacttctc gcgcgattcg gccggcaaat gcgccatatc gcgttcgatg acggcccggg
     2401 aattgggcca ggttgagttt tggtgcgggt agtcgttcga ccacatgcag ttatcgacac
     2461 cccatgacga aaacattttg ccaccgacga cgtcattgaa gaatgtcgcg tagatctgct
     2521 cggcaaagta ttcactcggc aatttgctca tctggacttt ttccgagtgg cgatgacgtt
     2581 tgtaaccttt gtcggcttga cccagccaga atggcatcca acccacttcg ttttccaccg
     2641 agactatttt gagcttgggg tggcgctcga gcacgccgtg aaaaatcaat tcgaacagcg
     2701 cgttttcgat ctcgcgggtc tgttcgagac ctatgcggta acgcttggga ccatgggcag
     2761 tctgccgatg catgctatcg ccgaagccgg tcaaaatatg cagatgtacc ggtagatcga
     2821 gatcctggga agccgcccag aatttttcat aatgctcgga tgtaaacggc aaactcggat
     2881 gaggcacctg ccagatcatc gtgccgacga cgccctgctt cttacagcgc tccatttcgg
     2941 cgatggcgtg atcgacgtta tacatggaga tcaatgccac gccgtaaagg cgatcgggta
     3001 ccttctgaca gtaatcgata atccagtcat tgtaagttcg gaacagacct tcttgcagag
     3061 cggcgtcttc gacgcaatac aatcccaagc ccaagctcgg atagagcact tcagccgcga
     3121 caccgtcggt catcatatcg gcctgacgtt cgagcggatc ccagccgccc ggccgggcga
     3181 cggcgaagcc tgccttgcga aacttctcga cattctccgg ttgctgaccc gccatgaata
     3241 agccgccgac cgcttgagtc tgaacttccg gcgaaccgaa catccatgcg tctctttttt
     3301 catcgaagaa catctttggc gcgcgcgcgc gcattgccgc cggcaagcct ttttcccaaa
     3361 gatccggcac ttcgattacg tgagaatccg acgagacaat tgattgttcc ataacgactc
     3421 cttttcccta tcttttagcc cagatttgat cccaaaagcc actcttttcc atatcattta
     3481 aaaaccgcgc gtcagtcagt tcctgtggct tcaccttttt ggcccgctcg tccgtctgcg
     3541 ccacttcatc gaggatcgcc tgaaacgctt cggctttaag tgccagccgc ggctccataa
     3601 ctttaatctc cgcgttatag gctgcttcca tgatcgcccg gtcgttgatg cgcaactgct
     3661 tgcccaaaac tttgtaaacg aattcgcggt cgctatgcat gatcttcgac gcttcgccca
     3721 ttgcccgcag gtactgcgcc atcgcttggg gtctgcgcgc aatcgtcgag cgtttcgtga
     3781 caaacgaggt ggcagcatag ggaatcttta gatcgggtcc gtatacgacg tagtgatatc
     3841 cgcgcgtcag cgcttgcacg tcggtaggcg gagtaaggca ggcgacctcg acgcctccgc
     3901 tcatcaacgc gatgagcgac tccggatcgc cgccggattg caagaaagtg taatcacgat
     3961 tgggctccat gttgtggcgg cgcaaggctt ggatcgtgaa ataatgcgag ttgctgccga
     4021 tgcggctgac tgcgatgcgc ttgcctttca aatcttccgg acgcttcaaa ttaccaccgg
     4081 cgacgatatt ctgcgtcatg aggttcttcg cgctgccgac gtaagcgatt tccgtcgaac
     4141 cgcggacgta ggcgatgacg ttgccgacgc cgccggtcat ggtcatgtcg gggtcgccgc
     4201 cgagcaatgc cgcggtgacg atgcttgaag atgcaatgaa aaccagactg tgatcgaggt
     4261 tgtatttttt aaatagtccc gcctcttcgg caatccacgg caacgcctgc gccatcaccc
     4321 gcgccgaata gatcgtcgta aacttttccg ctgcacggca gtctgccggc aacgttgcca
     4381 gcatccccag tacgacaatc aataaagcac gccgcattac ttcttcgtcc aaagctggtc
     4441 catgaagccg ctcttgttca tctcgtcgag atagcggcta tccaccatat ccgccggttt
     4501 gatcgtcttc gcgcgcgcat cggtcggcgc cagttcgtca agaatcgcct gcaaagattc
     4561 cagcttgatc gtcaagcgcg gctccagcgc tttaatctca gcattgtagg ccgaatcgag
     4621 cacgctgcga tcctggatgc gcagatactt acccaacact ttgtagaaaa attcccggtc
     4681 ggtatgcacc agcttggacg tctccgccat ggcgcgcata aagttgccga tcactgccgg
     4741 ccgcttggcg atcaccgagc gcttggtcac gaaggccgtc gccgaatagg gaatttttaa
     4801 atccgggccg taaactacat aatgataccc tttggcgatc gccatcgcat cggtgggcgg
     4861 cgttagattg gcgacttcca aggatccgct catcaacgcc gcgagtgttt ccggatcgcc
     4921 gccggtctgc agaaaactgt agtcgcggtt gggctccatg ttgtgccggc gcagcgcctg
     4981 aattgtgaag tagtgcgagt tgccgccgat gcggctgacg ccgatgcgtt tgcctttcag
     5041 atcttccggc tttttgatat tgccgccggc caccaaggtt tgcgtcatga cattcttgac
     5101 cccaccggtg aagaccacat cggtagaccc tttgacgtag gcggcaacgt tgccaatgcc
     5161 gccggtcacg gtcatttccg cgtcgccgcc gagtaaggcc gcggtgacga tgctcgatga
     5221 agaaatgaac accagattga aatcgagatt gtattttttg aagatcccgg cttcctgagc
     5281 gacccaggga aaggactggg acatgacacg ggatgaatgg ataccgacca gcttgtccgg
     5341 cgctcccgcg gcgatggttt gtccggccga cacgagcacc aggaagagcg ctgcgagcct
     5401 actcacgttg accataaagc tcctttacaa aaccactcag atctaactcc ttaaccaagc
     5461 ggttgtcaaa gaacctcgtt ggagtcttgg cagcgttttt gggctgctcg gcgatgccct
     5521 aagcgcattg actagtgccc aatggcacgg acgagatcac tgcggtactc ccaaccgcca
     5581 tttgaacaca cggcagctgc ccccagatcg gtacgtggat agtctgggcg gccatcgccg
     5641 cagcgcacct cgaaatagat ttcgtttacg agaattgtta gaagacggat cgggccgata
     5701 caaaactctc ggttttggtg tgtccggcag acagttttat cgtccaccat atagctcggc
     5761 gataaagcca ctgtcttcga gttcctttaa ccagcggttg tcgaaaaaat ctttcggatc
     5821 ggcgccggct gcgcgcgggt gatcggcaac agcgagtatc gaacgcacct ggtcgtcggt
     5881 ggtgtacgga acctttggat agactccgcg ataggcctga taagcttcat ccaagatcgc
     5941 cgagtcgccg gtcgcaagat acttggccag cgcccgcttg ctgatatcgc tatcttcatt
     6001 tacgattttg atagcagcga cataaccgcg aaggaacgcc ttgatccgct ccggttgttg
     6061 gcgcagaaat gcgcgatgag cgacgatacc caattgaatg tagggaatct tcaaattcat
     6121 gaagttgacc aattccttgt agcccatgcg ccgcgccatc aaagtcgtcg gcgcagaaat
     6181 cgtcgtcgcc gaaacgatgc cgcgatccaa ggcggccagc ccttcgcgta cgccgcccag
     6241 atacaacact ttgacatcct gccccgagcg caaattgtat tggcgcaaga gcgccgtcag
     6301 gccatgatcc gtggatgcgc ccttggtcat caagccgacg accttgccct tgagcgaagg
     6361 aacatcttta acgtcgttgc gaacgtaaag ggaaaatcca taggtcggca cgctggcggc
     6421 aatgtaagtt agatcggcgc cactcaaaac cgcattgatc accgcgccgc cggtggccgc
     6481 caactcgatg tcgccagaga ccagcgtctg cgtcaaacga gcgccgcccg aaatataaat
     6541 attttgcagg tcgacgccgt atttctgatc gagcttggtc tcggccatga cccagatcgg
     6601 caacatcgcc ccggaaggtt gtggatagcc gatacgaata gtgttagcgg aggcgccata
     6661 cgcggtagaa gcgacgaaag gcataataag gatcgcactg aggaaaaata tgcccgctgc
     6721 gatgcgatcg ccattaccaa gacactcacg gcgcaggcgc agtgaacgcc gagaaaaggt
     6781 ttttccatat cgaacgctgc gccctccgcc cctgcgcagc gaatccgccg aacacttaac
     6841 ggcggccata gagctcctgg acaaacccgc tgtcttctat ttcttttaag aaccgcgtat
     6901 cgaaaaagtc cttggggttg gctgccgccg ccttgggatg ctcggacaac gcgaggctgg
     6961 acttaatctg atcttcggta acgtagggca cccgcggaaa gattccccta aacgattggt
     7021 aggcctcttc cagtatcgcg ctatcggtag tgactaaata gcgactgagt gcgcgtttac
     7081 tggtttcggc gtcttcgttg gcgattttaa tggcggccat gtaggcgcgc aaataagcct
     7141 ttatcctttc cggctgttcg cgcgcaaacg cccgccgggt cgccacgccg ctgtgcatgt
     7201 aaggtaaacc gagagacgcg atattgacca actctttctg gcccatgcgg cgggccgcca
     7261 aagttgtcgg cgacgacaac accccggcgg cggcgatgct gtgctcgatg gccgcgagga
     7321 cttcgcgcac accgccgaga taaatgaact ttacatcctt ccctggttgt aaattgtagc
     7381 gtcgcagcag agcgatcacc gcatgttcag aggacgcgcc cttgctcatg atcccgatga
     7441 ctttgccctg caaactcgcg atgtctttta cctcaggccg ggcgtagaaa gaaaaaccgt
     7501 aggtcggcac gccgacggcg attgcaacca gatcgccgcc gcctagaacg gtattgacca
     7561 cggccccgcc aatgctggcg agatcgatat cgccggcaac catcgcttgg ttcatgcgcg
     7621 cggcaccgga gatgaaaata ttctgcaccg gcacgccgta tttttggtcg agtttggcct
     7681 cggcgacgag ccaaagcggc agcatcgcgc cggaaggctg cggataaccg accctgatcg
     7741 tcgcgctggc ggaatagagc tttgcctcag cgccgattat tagggcgatg acggccagga
     7801 gcagctttag gcaagagctc aatttcatcg ccggtcctaa ataacccgcc gacgactcac
     7861 gaccagaagc gctgcttcca caacgcgatg gcgtccggat tgaccactgt gtcaggcact
     7921 ttaccttcga gaattgccaa aatactttgt gccgccctcg tctgccccgc ctcgccggaa
     7981 cccggattgg cgccgatgtt gtgcggtgtc aggatcagct tgaagggatc aactttggac
     8041 aaggggccat ctttgggcag gggttcttgc tcgaagacat cgagtgccgc gccggcaatg
     8101 agattgccgt tcaacgcgcg aatcaaatcc gcctctttga tcgccgggcc gcgcgatgtg
     8161 tttatgataa atgcggtggg cttcatcatt gtcagttcgc gcatgccgat catgccgcgg
     8221 gtttcgcgcg tgagcacgac atgaagcgtc acggcgtcag agcttttcag cagttcgtcc
     8281 aagccgacca tcctgatgcc aagggacgcg accgtttctt gcttcacgta ggggtcatag
     8341 cccagcactt tcatgcccca cgcatccaat ctcttggcga ccgcctggcc aattcggccg
     8401 acaccgataa taccgaccgt ccggccgagc atcagagtgc cgcggttttc caggtctttc
     8461 caaccgccat tgcgaatgac cgcttcgttg agtttcagcc ttttgaacaa cgcgaccatc
     8521 aaacccatgg tcgcttccgc cacacccaaa tagttttccg gcgtcgggct gttgcagcac
     8581 aagatgccca gatccgtggc ggcatcgaca tctacggtct cgatgccgat gctcgatttc
     8641 accacggcct gcagctccgg gcaggagtcc atcacatcgc cgccgatata atcgcgcccg
     8701 gaaggataaa tgatctggct gtcgccgacc agattgatca attctttgcg ctcgtagcga
     8761 aagttgcgaa agtcttcctg cggtttgccc aacaccagct cgcatccggc ttcgcgcagc
     8821 gtgttttcaa cgattttgat gcgcatcgga ccggtcaagc agactttgcg tttggcggcc
     8881 atctttcacc tcgctacaag ttgtagagtt tttttgccga ctcggcgaac agccatctct
     8941 tcgcttcttc gttcaggtct tcgcgcttgt ttaaatgcgc aatattgtcg ggaaactcat
     9001 tgtcccaatg cggaaagtcg gaggcataat aaatattacg atgggagaga attccagtag
     9061 tggcgccgat caacggctcg taatcttcgg cgtggaaata aatgcgccct tctttgagat
     9121 actcgatggg cttctttttg cacatcggcg cttccacttc gccgcgaatc tcccactcct
     9181 cgctcatacg gccaaaccag tacggcgccc aactgcatcc cgcttccatg aatgctacgc
     9241 gcagtttggg aaaccgatcg aagacgccgc gaaacatgag actcgtgcaa tgcatcatct
     9301 gcgcaaacga atgcgacagc gtgtgcactt cgatgaattg gtcgaacaaa tcgccggcga
     9361 agtaatgcgg cccgcgcaca gtggcatgga cgcccaccat gcagtcgagt ttttccgcct
     9421 cttgataaat cggccagaaa ctttcatggc cgagcggcaa gcgtagcccc accgccggca
     9481 gcatcaaacc gaccaatttt aattcattca ccgcccggcg cagctctttg accgcctcgg
     9541 atacgtcctg aaacgatacc aaggcaacgc acttcaaacg cgggctcact ttctgaaatt
     9601 cttccgaaca gaagttattc caagcctggg catactgcac cgccacgtca ggctcgcgaa
     9661 tccagccgac gctcaagcca ttggtcggat agagcacggc cgtcgacacc gagccgcggt
     9721 ccattgcctc cagccaactc cgcgcatcgc cggcgcggct gccaagctta ccgctcagcg
     9781 aacggtccca actgtcaccc ggcgttagcg acgacacccg tttgacgtat ttttccggca
     9841 tgtatttttt gagctgcgcg tcgctttcgg tgatgtgtcc gtcggcatcg atgacttcga
     9901 actggcccgc cataattaat tcctcccgct cgcgtaatga ttcggcttac gcctcatcgc
     9961 ctcaccgtgt caagagaaac caagcactgc cattggcgac gaattttctt gagtcaaacg
    10021 gctaaaagat gtacctagga ggacggttcc aatctgctga cggcatcttg atgaaccaaa
    10081 ctcgcggcta taactttatc gagcgtccat ggtagacggg tgtcgaagtc tgtcggccgg
    10141 tttatgatca gccgccgagg aacaacccga accatgcgcc gatcgcctat ttcgatttta
    10201 ttcgcttgca atagcgccca aacgacagaa ataatccact tacacgtaat cgctatcaga
    10261 ccgtctaaac tgacgaacct aacgagggat atatgaaaag aacttgttac ctgacgctgc
    10321 tgatcacctg gacgctctgg acgcggacga tatcacagac atccgatacc tggtcggccg
    10381 ctcccggctt ggcgtctcaa gataaatgtc tggccagtgc caaggacaaa ctcgatatgt
    10441 ggaagcagtt taaggatgcc aagtttgaaa ag
//
LOCUS       DATLCH010000161         8454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5664985, whole genome shotgun sequence.
ACCESSION   DATLCH010000161 DATLCH010000000
VERSION     DATLCH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5664985"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="VMZ02_04695"
     CDS             complement(<1..2287)
                     /locus_tag="VMZ02_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HUR71261.1"
                     /translation="MSIVIALFARQGSAQQPSITYVYDDLGRLVRVITAGGDAATYHY
                     DAVGNILRITRETGVAATASVISVSSTAGVRGTSLLLTITGLNLAGANLGSSVGDIAF
                     TNIRTSLDQITTDMSVTPSAPIGVAQIFVETQFGTIPIAFTITDSAPAVAIVTPAEGS
                     STIENGQVTLTAQAADNVQVTQVSWTLNGVPGPVVTSAPYQAIITTPINITSLNIQAT
                     ATDSVGQTTSASRAITVLADPPPMVVITSPLAGATVLEGSQLNLSANATDNVQVAQFV
                     WTVNGVEQNPIFSSPYEKIVNVPLGITSLTILGKAIDNLGRSTTATRTVTVLATPKTT
                     VIGRVVSNLGQTIAGASVSVFVQYTALSQNDGSFTISNVPTNQGNISATAQAIIGGST
                     RTGVSASVPPVVGATTNVGDIVIAGSAIADLYPAPIIGIDAAAYLAVTDLNGDGILDI
                     VAPNISTSDVWVFLGTGAGTFQTEQRFPAGNSPWAIAAADLNGDGILDLVTTNTSTDD
                     VSVLIGNGNGTFQSQQRFPVGDFPYSVALSDLNGDGRRDIIVANQNSNDVSVLLGNGN
                     GTFQPQQRYSVGLTPYWIAVADLNRDGFPDIVTPNANSNNISVLIGNGNGTFQAQLNF
                     PTGSSPSSVNVADLNGDGLQDIAVANNFSDDVSVLLGNGDGTFQIQQRFFVGGGPVGV
                     TIADLNGDGVRDLVTANQGDVVLSALLGSGNGTFQNAQSIPVGRAPQFVALADVNGDG
                     FQDLVTSMPFTDQILLSFGNGD"
     gene            complement(2931..3953)
                     /locus_tag="VMZ02_04700"
     CDS             complement(2931..3953)
                     /locus_tag="VMZ02_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71262.1"
                     /translation="MIKRLLLVGFLVGGFLSSDAPVAAQDQFYRGKVVRVIVGFPPGG
                     GYDTYARLIARHMGKHVAGNPTIVVDNMSGAGSMISANHIYRIAKPDGLTVGHFIGGL
                     FLQQILGKPGIEFDARKFEYIGAPAQDNFILGLHKNTGFTSLQQWIAARTVVKFGGAG
                     FGAGTDDLTAVARDVMGLPIQLVAGYKGTADIRLAFNNGEILGITNSWQSTRSTWRTE
                     LDGGTLNILVQLASKSHPELTKYPLPGDLIKTDEGRKIFQAVAQVHGASVRPFVLPPT
                     TPKERVQMLRKAFTDTLTDSDFVAEAKKGNIEINPVNGEELAKSVHSVLQLEPSLVAK
                     LKEILK"
     gene            complement(4024..5064)
                     /locus_tag="VMZ02_04705"
     CDS             complement(4024..5064)
                     /locus_tag="VMZ02_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71263.1"
                     /translation="MPNRIIAKNFLVFTLLWIGLADLANAATTDDGFYRGKTVRLIVA
                     FSAGGGYDTYSRTIARHLGKYIPGNPTVVVDNMTGAGGIIHANYMFKQAKPDGLTIGN
                     NSGGLFLQQVMGAKGIEFDGRKFEYLGAPAVDHLVCAIAKASGVTSMERWFAAKEPVR
                     FGGVGPGGFASDLPRLLQTALGLPIRVIDGYKGTSDIRLATESGELAGSCLSWDSYKT
                     TWRKTIESGEVNPIIQVMPKKHPELANVASAMDYAKTEEAKKLIKHGGYDLAYSARPY
                     FLAPGTPKARVQILQKAFADVLKDAELLAEAKKANFVIEYVSGEELESIVHGLYKIEP
                     AFAARMKQILVP"
     gene            complement(5084..5431)
                     /locus_tag="VMZ02_04710"
     CDS             complement(5084..5431)
                     /locus_tag="VMZ02_04710"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HUR71264.1"
                     /translation="MEELTEQKCVACRVGAPSVTAEEVKELQPRVPDWQIINEDNIPK
                     LDRLFKFKNFKDAIAFTDAVGAAAEEEGHHPRLTTEWGKVAVTWWTHKIKNLHKNDFI
                     MAAKTDAIYRKFE"
     gene            complement(5545..6597)
                     /locus_tag="VMZ02_04715"
     CDS             complement(5545..6597)
                     /locus_tag="VMZ02_04715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71265.1"
                     /translation="MKCSLFTEIQCPQGSSPVERLAEFMDQAELADQLGYDTYWVAEI
                     HCQPRFSLMSAPYVALGAVAQRTKHLRLGVAVNTLPVHHPVQLAEQAAMLDLVSGGRM
                     EFACGGGHPHSRAYECFGADHKATHEVMAEAMEVIRKSWSDEKLIHNGKFFQIPEVIV
                     NPKPVQKPLPPFWMATSSLDGVEVGARLGVNIFLPIHTRLPEQVYEFADAYWTGLKKH
                     GHERHSRSLGLLVPMHIAATTENARARAQTGVMSYFKTILDLRLDYTEWLNRRGIELP
                     ARLRTAAGTMVGYETVCDKHAVIADAPLALEKLQALCARTGASQLLCWFNIGTMAHAD
                     VEDSMRRFAADVMPKL"
     gene            complement(6818..7660)
                     /locus_tag="VMZ02_04720"
     CDS             complement(6818..7660)
                     /locus_tag="VMZ02_04720"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HUR71266.1"
                     /translation="METIGMIGTGAMGLALLERLKLAGVGNVVCYDVFGPALEDARKH
                     GFSAATSVAEVGKRATLIDVVVRTDQDMLDCMLGPDSVIEAALPGTLVLLHSSILPQT
                     IKNIEATARAKGVHVIDACMTGVPNTVRAGKLSFVVGGADELVERARPHLLKMSQEVF
                     HMGPIGTGAVGKLIKNMLGGAETLIVHEAIQIGIAGGIAYPKALEMMRRIGHDSVLNR
                     WQNTFDPSGSDPLPRSGRNVLNKDIPLVAELARLLNTDTPITQQLAAAAERVVSANEK
                     RNKS"
     gene            complement(7681..8172)
                     /locus_tag="VMZ02_04725"
     CDS             complement(7681..8172)
                     /locus_tag="VMZ02_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71267.1"
                     /translation="MFDGWSYSQVLRVIGQSLEERGVEDFSLAWQDGEFVVRGKRAAP
                     LERRWIDKLLGQKAGHVERNVEIHYALKSILWLQIRGEGMRKDPNQIPDYFRLSQTLR
                     TVGNYIENRAMPIVSLRRGGGTLYLEFQERSGKKRVEEHGMGSFENYFLRTYLRRRND
                     RRT"
BASE COUNT         1886 a         2414 c         2238 g         1916 t
ORIGIN      
        1 cgtcgccatt gccgaaggac agaagaatct gatccgtgaa cggcatcgat gtgacgagat
       61 cttgaaagcc gtcgccattg acgtcggcga gggcaacgaa ttggggcgcg cggccaaccg
      121 ggatactttg tgcattctga aacgttccat taccgctgcc cagcagcgct gagagaacga
      181 catcgccttg attcgctgtt actagatcgc gcactccgtc accattcaaa tccgcgatcg
      241 taacgccaac aggaccgcct ccaacaaaaa atcgctgctg aatttgaaac gtaccgtcac
      301 cattgcccaa gagtaccgaa acatcgtcgg aaaagttatt cgccacggca atatcttgca
      361 agccgtcgcc gttaagatcc gcgacgttca ctgagcttgg actactcccg gtaggaaaat
      421 ttagctgagc ttggaaggtc ccgttgccat taccgatcag cacggagata ttattggaat
      481 tggcattggg ggtgacgata tcaggaaatc catctctgtt gagatccgcc actgctatcc
      541 agtacggagt cagaccaacc gagtaacgct gctgcggttg gaaagtaccg ttgccgttgc
      601 ccaataatac cgacacatcg ttggagtttt gatttgccac aataatgtcg cgccgaccat
      661 cgccgttcaa atcgctaagc gcgacggagt atggaaagtc gcccactggg aaacgttgtt
      721 gcgactggaa agtgccgttg ccgttaccaa ttaataccga cacgtcgtcc gtgctggtat
      781 tggtcgtaac caaatcaagg ataccgtcac cgttgaggtc cgcagcggct atcgcccatg
      841 gagagttccc ggccggaaag cgctgctctg tctggaacgt gccagcgccg gtgccaagga
      901 atacccaaac gtcgctggtg gaaatattcg gtgccacgat atcaagaatg ccgtcgccgt
      961 tcagatccgt gaccgcgaga taggcagctg catcgatgcc tataatcggt gctggataga
     1021 ggtcggcaat cgcacttccg gcaataacaa tatcgccgac gtttgtcgtt gcaccgacta
     1081 cgggaggcac agacgccgac acgccggttc gggtcgatcc gccgataatc gcttgcgccg
     1141 tcgcgctgat gttgccctgg ttagtcggta catttgaaat agtgaagctg ccgtcgtttt
     1201 gactgagggc agtgtactgg acgaatacac ttactgatgc accggcgatc gtttggccga
     1261 gattgctgac gacccgtcca atgacagtcg ttttcggcgt cgccaaaacg gtgaccgttc
     1321 gagtagccgt cgtactccga ccaagattat cgatcgcctt ccctaaaatt gtcagggacg
     1381 tgatacccag cggcacgttt acaattttct catagggcga gctaaagatt gggttctgct
     1441 cgacgccatt aaccgtccag acaaactggg ctacttgaac gttgtcggtg gcgttcgccg
     1501 ataagttaag ctggctaccc tccagaaccg ttgctcccgc taaaggcgag gtaatcacaa
     1561 ccatcggcgg tggatcggct agtaccgtta tcgctctgga cgcgctagtg gtctgaccta
     1621 cactatccgt cgcggtcgcc tgaatattca aagacgtgat gttgatcgga gttgttatga
     1681 tcgcctgata cggagccgat gtgacaacgg gaccaggaac tccattcaac gtccacgaga
     1741 cttgcgtaac ctggacattg tccgctgcct gggcagtgag cgtcacttgg ccgttttcaa
     1801 tagtcgaaga cccctcggct ggcgtcacga tggcaaccgc tggtgcgcta tctgtaatgg
     1861 taaatgcgat ggggatagtg ccaaactgag tttcaacaaa aatctgcgcc accccgatgg
     1921 gtgcggatgg agttaccgac atatccgtcg tgatctggtc gagagaggtg cgaatgttcg
     1981 tgaatgcaat atcgccgacg ctcgacccaa ggttcgcgcc agccaggttc aaacccgtaa
     2041 tggtcaacag caatgacgtg cctcgaacac cagctgtcga cgatacgctg attacgctgg
     2101 ctgtcgcggc tacacctgtt tcccgggtga ttcgaagaat atttccaacc gcatcgtaat
     2161 gatacgtcgc cgcatcgcca ccagctgtga taacacgaac tagcctacca agatcatcgt
     2221 agacgtatgt gatactcggt tgctgagccg agccctggcg agcgaataat gcgatgacaa
     2281 tcgacacaag gagcaaagat actgaatacc tacaggatcg gctgttgtcg gcatttcgca
     2341 aggtgtgcat acaaagaccc tctcagacga tgcaatcgtt tgtataaatt aacctgtcac
     2401 cctatttcag tgaccgatta gttgttcgcc gttgtttttc ccctctgccg cacaggtagc
     2461 tgatctttga aaaacgctgt cgacaaaact caccctagtg caattcgtac ttattttgtg
     2521 tcaaaccaac tcaagctccg gttgttcgtt aactgagcta tgtgcacact catcgatgcg
     2581 agcgaacctg gcgacagtcg gatatacgct ccgcgccgat ttagatcttc tcgaaaatga
     2641 accggaccga aattttcgcc actgtactga aaatattagg ggtatggcaa gggttgccaa
     2701 aacaggtcat tgacggcaat caatgcaatc tcgcgacccg ccattgcaag ataggaaagc
     2761 ccacttgtcg cgatacgaca ttcgcagcag gcctaagtcg attttatgac tgggtaggcg
     2821 gcggatacca ggttttatct aaatgtcggc caaacaaatg cgggaattag aaaatggcca
     2881 gcgggaatga accggttagt tcacgatgat gcaggccaac ttggaccaca tcacttgaga
     2941 atttctttaa gcttcgccac caggcttggc tccaactgta agacactgtg cacgctcttg
     3001 gctagttctt cgccgttaac cgggttgatt tcgatatttc ccttcttcgc ttcggcgacg
     3061 aaatcgctat cggtgagagt atcggtgaag gctttacgaa gcatctgcac gcgctctttg
     3121 ggcgtggtcg gcggcaagac gaacggccgc accgaagcgc catggacttg ggcgacggcc
     3181 tgaaaaatct tgcgcccctc gtcggttttg atcagatcgc cgggcagagg atacttggtc
     3241 agctcgggat gagacttgct ggctaattgg acgaggatat tgagcgtccc gccatcgagt
     3301 tcggtgcgcc aagtcgagcg agttgactgc caggagttgg taataccgag aatctcgcca
     3361 ttgttgaagg ccaggcgaat gtcggcggtg cctttgtagc ccgcgaccaa ttggatcggc
     3421 agtcccatca cgtcgcgcgc caccgcggtg agatcatcgg tgccggcccc gaaaccggcg
     3481 ccgccaaact tcaccacagt tctcgcggca atccactgct gtaggctcgt aaagccggta
     3541 ttcttgtgca gccccaagat gaagttatcc tgcgcgggtg cgccgatgta ttcgaacttg
     3601 cgtgcatcga attcaatgcc cggcttaccg agtatctgct gtaagaacaa accgccgatg
     3661 aaatggccga cggtgagacc gtcaggctta gcgatgcgat agatatggtt cgccgagatc
     3721 atgctgccgg cgccgctcat gttatcgacc acgatggtcg gattgccggc gacatgcttg
     3781 cccatgtgcc gagctatcaa tcgcgcatag gtgtcgtaac cgccaccggg cggaaagccg
     3841 acaatgacgc gcaccacctt gccgcgataa aactgatcct gcgccgcgac tggcgcatca
     3901 ctactcagaa atccaccaac taaaaacccc acaagaagca gtctcttgat cattccgcac
     3961 tctccgcccg cgttcggacc ttgaactttt gaacccttga aacttgaacg gcgtttccca
     4021 tccctatggc acgagtatct gcttcatccg cgcggcgaac gccggctcga tcttgtagag
     4081 cccgtgaacg atgctctcca attcctcgcc ggaaacatac tcgatgacga agttggcttt
     4141 tttcgcctcg gccaacagct cggcatcttt gagcacgtcg gcaaacgctt tctgcaagat
     4201 ctgcacgcgc gccttgggcg ttcccggcgc gaggaaatac ggccgcgccg aataggccag
     4261 atcataaccg ccatgcttga tgagcttttt tgcttcctcg gtcttggcat aatccatcgc
     4321 cgaagcaaca ttggccagct ccggatgttt cttcggcatg acctgaatga tcggattgac
     4381 ctcgccggac tcgatcgtct tgcgccaggt ggtcttgtag gagtcccacg ataaacaact
     4441 gccggcgagc tcgccgctct cggtcgccaa acgaatatcg gaagtgccct tgtagccgtc
     4501 aatgacgcgg atcggcagtc cgagagccgt ctgcaatagg cgcggcaagt cggaagcgaa
     4561 acccccgggg ccgacgccgc caaaacgaac cggctccttg gcagcaaacc agcgctccat
     4621 cgaggtcacg ccgctagctt tggcgatagc gcagaccaag tgatccactg ccggcgcgcc
     4681 gagatactcg aatttgcggc cgtcgaactc gatccccttc gctcccatta cctgctgcaa
     4741 aaacaaaccg cccgaattat tgccgatggt gaggccgtcc ggtttggcct gcttgaacat
     4801 gtagttggca tgaatgattc cgccggcgcc ggtcatgttg tcaacgacca cggttgggtt
     4861 gccgggaata tatttgccga gatgccgtgc aatggttcgg gagtacgtgt catagccgcc
     4921 gcctgccgaa aatgcgacga tcagccgcac tgttttaccg cggtaaaacc catcgtccgt
     4981 agtcgcggca ttagcaagat ccgccaggcc aatccataac aacgtgaaca ccaggaagtt
     5041 tttggcgatg attcgattag gcatcactcc accactccca cctctactcg aacttacgat
     5101 agatcgcatc ggttttcgcc gccattatga aatcgttctt gtgcaggttc ttgattttgt
     5161 gcgtccacca ggtcaccgcc accttgcccc attcggtggt cagccgcggg tgatggccct
     5221 cttcttcggc agcggcgcca accgcgtcgg taaacgcgat ggcgtcttta aagtttttaa
     5281 atttaaacag acggtcgagc ttgggaatat tgtcttcgtt gatgatctgc cagtcaggca
     5341 cccgcggctg gagctcttta acttcttccg cggtgaccga cggtgcaccg acccggcagg
     5401 ctacgcattt ctgttcagta agttcttcca tgtttgactc ctttcaacaa aacttttacc
     5461 cagagaagca gagaaagccg acagagtttc aaagccactc cacgttaggg acttgcggtg
     5521 aacgtttcgt ttgcttgttc ccaatcaaag cttcggcatc acgtcggcgg cgaagcgtcg
     5581 cattgagtct tcgacatcgg cgtgggccat ggtgccgatg ttgaaccaac agagcaattg
     5641 cgacgcgccc gtacgggcgc acagcgcttg cagcttctcc aaggccaggg gcgcatcggc
     5701 gattaccgca tgcttatcgc aaacggtttc gtaaccgacc atggtgcctg ccgccgtgcg
     5761 caagcgggcc ggcagttcga taccccggcg gttgagccat tccgtgtaat cgagccgcag
     5821 gtcgagaatg gttttgaagt aactcatcac gccagtttgg gcgcgcgccc tggcattctc
     5881 cgtggtcgcg gcgatatgca tgggcaccag caatcccaac gaccggctgt ggcgttcatg
     5941 gccatgcttt tttaaccccg tccaataggc atcggcgaac tcgtaaactt gctcaggcaa
     6001 gcgcgtatgg atcggcaaga aaatgttcac acccaggcgc gcaccgactt cgaccccgtc
     6061 gagcgagctc gtcgccatcc agaatggcgg cagtggtttt tgcaccggtt tgggattgac
     6121 gatcacctcg ggaatctgaa aaaacttgcc gttgtgaatc aacttttcat cgctccaact
     6181 tttgcggatg acttccattg cctccgccat cacttcgtgt gtcgccttgt gatcggcacc
     6241 gaagcattca taagcccggc tatgcggatg gccgccgccg caagcaaatt ccatgcggcc
     6301 gccgctgacc agatcgagca tcgccgcctg ctcggccagc tggaccggat gatgcaccgg
     6361 caaggtgttg actgctacgc ccaaacgtaa atgtttcgtc cgctgggcca ccgcgccgag
     6421 cgcaacatag ggtgccgaca tcaaggaaaa ccgcggctgg cagtggatct cggcgaccca
     6481 ataagtgtcg tagcccaact gatcggcgag ctcagcctgg tccatgaact ccgccaagcg
     6541 ctcgaccggc gacgatcctt gaggacattg aatttcggtg aatagactgc atttcacgat
     6601 gatattattc cttcttggta cgtctagttg agtgtttgca taggtttcgg attcgtatat
     6661 tcgtcccgcc aaaccgcaac gacgccaact tcacaatcgc cgtcatttcg accgagggga
     6721 gaaatctttc ttacattctt cgcgttcctc gtgctgcctg agatcgtccg taatcatttc
     6781 cgtttgcgcg ggaagctgcc aatatccctc agcggattca cgacttattt cttttttcat
     6841 tggcgctgac gacgcgctca gcggcggcgg cgagctgttg ggtaatcggc gtgtcggtat
     6901 ttaatagccg ggcaagttcg gccactagcg gtatatcctt gttcaacacg ttgcgcccgg
     6961 agcgcggcag gggatcgctg ccgctcggat cgaatgtgtt ctgccagcga ttcaagacgc
     7021 tgtcatggcc gatccgccgc atcatttcga gagctttagg atacgcaatg ccgccggcaa
     7081 taccgatctg gatcgcttca tggacgatca atgtctcggc gccgccaagc atgttcttga
     7141 tcagcttgcc gaccgcgccg gtgccgatcg gccccatatg aaaaacttcc tggctcattt
     7201 taagcagatg gggcctggcg cgctcgacca gctcgtccgc gccgccgacg acgaaactca
     7261 gtttgcctgc ccttaccgtg ttcggcacgc cggtcatgca tgcatcgatt acgtgcactc
     7321 ctttcgcgcg cgctgtggct tcgatgttct ttattgtctg cggcagaatc gagctatgga
     7381 gcaaaaccag cgttcccggc agcgcggctt cgatcacgct gtctggaccg agcatgcaat
     7441 cgagcatatc ctgatccgtc cgcacgacca catcgatgag cgtcgcgcgc ttgccgacct
     7501 cggcgacact agtggccgcg ctaaagccgt gcttgcgcgc atcctccagc gccggtccga
     7561 agacgtcata acaaaccaca ttaccaacgc ctgcgagctt gagacgttcc aggagcgcca
     7621 atcccatggc gccggtaccg atcataccga ttgtttccat agcgctccta ttgcatcgcg
     7681 ttacgttcgg cggtcgttac gccggcgcag ataggtgcgc aagaaataat tttcgaatga
     7741 tcccatgccg tgctcttcta cacgtttctt gccgctccgt tcttggaact ctagatacag
     7801 agttccgccg ccgcgacgca aggatactat cggcatggcc cggttctcga tgtaattacc
     7861 taccgtacgc agggtctgcg ataagcgaaa atagtcgggg atctgattgg gatctttgcg
     7921 catgccctcg ccgcggattt gcagccaaag aatactcttc aaggcgtaat gaatttcgac
     7981 gttgcgctcg acatggccgg ctttttgacc gaggagctta tcgatccagc gtctctccaa
     8041 tggcgcggcc cgtttgccgc gcacgacaaa ttcgccatcc tgccaggcta aggaaaaatc
     8101 ctctaccccg cgctcttcca acgactgtcc gatgacgcgc aagacttgag agtaggacca
     8161 accgtcaaac atagactagg ccgactccaa ccttggcaag aaaatcactg aactcaaacc
     8221 gcaatcccac tctctttcag cgtccgagtt cactcataac agcggcactg ccgcgcaaca
     8281 gcacttgtca ctgcgcctaa taaaattaaa tagcagctat tcttgggaga gtcgatcgaa
     8341 gaattcagtt gggcgttcgc cggcgatgtc aaattagata gctagcttag ctaccgtcga
     8401 aggagtgcag aattttaact ctccggcaaa aaaatgcagt ggttaccaac ccaa
//
LOCUS       DATLCH010000162        10315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1938908, whole genome shotgun sequence.
ACCESSION   DATLCH010000162 DATLCH010000000
VERSION     DATLCH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938908"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VMZ02_04730"
     CDS             complement(<1..843)
                     /locus_tag="VMZ02_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HUR71268.1"
                     /translation="MQPVPESKIINEPRGLKTKLTDYSSLVRFSHTVFALPFALSSVA
                     LAWPRHPVTLRSLLWILVAMVGARTAAMGFNRLADRKFDALNPRTKNWELPHGKVTVL
                     EATLLTIFASLVFMLAAYELNWVCFVLSPFALAIVFFYSLTKRFTWTAHLFLGLALAI
                     APVGAWLAVAQPPFEVAELKVPIYLGLAVVFWLAGFDIIYSLQDRAFDEKIGLHSIPV
                     RFGVAGALRLSSAFHCGTILFLALIGNAAALGLLYWSGFLTVTAILVWEHRIVRPDDL
                     SRINK"
     gene            complement(846..2288)
                     /locus_tag="VMZ02_04735"
     CDS             complement(846..2288)
                     /locus_tag="VMZ02_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HUR71269.1"
                     /translation="MAYDNLTEFVQVLERAGELKRISHPVNAELEITEIADRVMKSAG
                     PALLFENVIGKTIPVLINAFGSTTRMALALGVKDIEEIASEITKLIQTRPPKTFKDKL
                     QLLGGLVKLAGIPPKIVKEAACHEVVIRDPDLNLLPVLTCWPGDAGPFITLPMVFSKD
                     PARGTRNVGLYRMQVFDRRTTGMHWHLHKVGARHFQQHKENKNKLELAVCLGGDPAMI
                     YAATAPLPPQVDEILFTGFLRKKGVELVRGVTVDVEVPANSDIVIEGYVDPAEPLRRE
                     GPFGDHTGFYSLADDYPVFHVTCITHRKNPIYPTTIVGRPPMEDAYLGKATERLFLPL
                     LRVTLPEIVDMNLPVHGVFHNLAIISIKKEYPAHARKVMHALWGLGQMMFTKTLIVVD
                     HDVNVHDLAEVTWVVGNHIEPKRDTIFSDGPVDVLDHAAPMLGYGSKLGIDGTRKWRS
                     EGFEREWPGAIVMDEKTKKHIDAIWKKLGI"
     gene            2311..4308
                     /locus_tag="VMZ02_04740"
     CDS             2311..4308
                     /locus_tag="VMZ02_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR71270.1"
                     /translation="MKSPKRATSAQPGRSKQAVRTQPFPTWVVPLCVALATLIAFFPA
                     LLNDFVNWDDDKILYDNPNYRGLGWVQLKWMFSTLLMGHYQPLTWLSFALDYQVWGMA
                     PFGYHLTSLLLHTANAVLFYCISRRLLGAAVGTLTQKHSWQIELSAGLSALLFAIHPL
                     RVESVAWATERRDVLSGFFYFMTLYLRAGENLPPSIQRRWLGAAAFTYLLSLLSKATA
                     ITLPVILLLLDVYPLRRLAGNPYDWFKRESREVFYEKLPFFILAIGFAITALIAQQVT
                     GALKPLEQFDVLSRLLQACFAYIFYLWKTIWPFGLAPIYELPIETGSWIWVFVFSAAA
                     MVGLTLVFYSLKRHWPMIFACWVYYIVVLAPVTGVAQSGPQLVADRYSYLACLGWPLL
                     LAGGFFRWSSERNVHVGGQRQFVAAAMLLVVGSLGFFTWQQTLVWRSPVSLWQHGTKT
                     EPLSSIAHYNLGRALERENNSTSAIDSYRRALAINSSYSKAHFNLARLLALDGREAEA
                     IAHYRRVIEIRPDHADAHNDLGLLLEMKGEDAAALAEFRRAVQLDADHLKASFNLAEL
                     LAKQGDLANATARYEQAGRLNPNDSLIQLRWAIALARQGQLDSATRHFRRAVELKPVD
                     ADARVLLARSLAAQGKHDEAERHYQDALRLSRTGGKGAANP"
     gene            4333..6285
                     /locus_tag="VMZ02_04745"
     CDS             4333..6285
                     /locus_tag="VMZ02_04745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR71271.1"
                     /translation="MKSLSRTSNQRSRHSTPPANVAADSSWIQWVIPLLLALAAFAVF
                     SSALSNQFVAWDDDLMLVDNPNYRGLGWPQLRWMFTTFHAGHYQPLSWITLGLDYLLW
                     GIDPFGYHLTNLVLHAANTVIFYFVCRQLFTRIFVAAREARWELDFSAALATIFFALH
                     PMRVESVVWATERRDVLSGGFFLAAIYCYLRAQQAAALPSIARNRWLGLAVAAQLLSL
                     MAKATGITLPLVLVLLDIYPLQRLPASVRTWFTADSRRVLVEKLPFLLLSLIFGVVAL
                     WAQQASGALRPVQLYFFSYRLGQAFYGICFYVWKTLLPIGLSPLYELPYDFEAWTGVF
                     FACAGITTLVSLLAFFRRRRWPWLAACWAYYVIVLAPVLGVAQSGSQLVADRYSYLSC
                     LSWAALLAGLSFYVLQSAKPGRMAIAVVGILVTGVGLGLLTRAQVGAWRDTESLWTHT
                     LRVTPNTAIANYNLGKFYETNGSAQKSMELYRRAVSINPTHTEAHYNLARLLGQRGQV
                     DEAIAHYREALKFKPNDVETYNNLGQLLALHGDLPAALAAYQKALAFDPQFAKTYFNR
                     GRLFVRSGEFDKAIDSFQRALDLTPDTAEIHAGLGDAYARAGNVDQAIIHLDAAIKLK
                     PQLPGVQAVLAQLLEVRARRVAGGKR"
     gene            complement(6418..6576)
                     /locus_tag="VMZ02_04750"
     CDS             complement(6418..6576)
                     /locus_tag="VMZ02_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71272.1"
                     /translation="MKLMRAAIGSSFDGDKSVDFVATLIALAASFGNLDGALVRSLRA
                     MEHDKSYY"
     gene            6668..8959
                     /locus_tag="VMZ02_04755"
     CDS             6668..8959
                     /locus_tag="VMZ02_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR71273.1"
                     /translation="MKWSDYRANPEHPPLAKLWAALPLLAFDIKDPRPSAMAWDIIPI
                     TRPHTTHTGSVAAKLLFVDNDGETLFFWAKVQMVLLALLLGGIIYLWSRRLFGIEAAT
                     ASLFLFSLDPNIIAHSQIVHTDVAFTALFFTGSYFFWRALERLTWSRLALSACFFGLA
                     ASTKYAYLAMLAVWGAVIVLRAFSAQPLECALGAPRVVVGRRQKAVLLCGIIFCSLIA
                     AYCLIWAAYGFRFDALPGNGRSLPMADELKTLPRLSGLISLLTQFHLFPEAWIYGQLF
                     VLNNLQREAYLLGAYSDHGFWLYFPIAFAVKTPLPTLILLLVTLIFWGMKKLDRAKVN
                     FLLVCLAVYFLLAILSRLSIGIRHILPIYPFLFVLVGGTVGQLWRARTAANRGAILLL
                     GLWSVWSTINIYPHFLAYFNELAGGPRNGHKVLLDSNLDWGQDLKGLKDWMVENQVNK
                     IQFLYFGFHNEAEPRYFGIDAQFLPGSWVSTAAIADKYAEPSDYLAISANHLFGRHYI
                     RGDRRVDFLKPFLSLSPAAIIGHSIYVYQISQAIGALRETVKKSPESAQVHADLGGLL
                     ENQGNTFDAEKHYRQAVQQKHPPTKALYNLGMILAKQNHLDEAIEFLQRASAASPGDE
                     DIRYDLAMTLAVRGDHERAIAELRETIRIEPLYTKAFYNLAVLLARQGQSREAIESLR
                     AAINADPLFAKAHYQLGVMLAQTAAHEESIRSYRRALQIEPTMAEAHQGLGKLLALTG
                     RTDEGVEHLNEAMRLIKLRPAAR"
     gene            9083..>10315
                     /locus_tag="VMZ02_04760"
     CDS             9083..>10315
                     /locus_tag="VMZ02_04760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUR71274.1"
                     /translation="MNRRFRTAKKGRDDPPAQGALTSRRRSGRLSMFWLVVGAAFFLR
                     LAYLWQIDAVPLFYNLAGDGRNYDEWAQRIAAGDWLGQGVFYQAPLYPYFLGVMEFFV
                     GHNLWLVRFLQIVLGAISCGLIFRVGERLFSRTSGVAAGLVLAFYSPAIFYDGLIEKS
                     ILDLTLLSLALFVLLVVSDNRWAKWLACGGLLGLLGLSRENALILVPVVAAWIIFHNR
                     ERSVALRARWLGLFFAGVSLVLLPIGLRNLLVGGEFKLTTSQFGANFYIGNNPVADGT
                     YGSIRNEIKETQLEGPDAKRLAERALGQSLTPGQVSSYWYHRALEYISGQPVQWLRLV
                     GFKWLLVWNAREVEDSDDFYIYKNWSWLLALLAWVSHFGILAPLASVGVLLTLRQWRQ
                     LWLLYAMIVALTASVAVFY"
BASE COUNT         2103 a         2690 c         2875 g         2647 t
ORIGIN      
        1 tttgttgatg cgcgataagt cgtccggtcg aacgattcga tgctcccata cgaggattgc
       61 cgtgaccgtc aggaatccgc tccagtagag tagccccaaa gcggcagcgt tgccgataag
      121 agcgaggaat aaaatcgtgc cgcaatgaaa agcactggac aatcttaatg ctccggcgac
      181 cccgaaccga accggtatcg aatggagtcc gattttttcg tcaaacgcgc ggtcttgcag
      241 tgagtagata atatcgaagc cagcgagcca aaataccacg gccaagccaa ggtaaatggg
      301 caccttcaat tcggctactt caaaaggtgg ctgtgcaacc gctaaccatg cgccaacggg
      361 ggcgatggcc aatgcgaggc cgagaaatag atgggccgtc caggtaaacc tcttggtcag
      421 cgagtagaaa aatacgattg ccaacgcgaa cggtgataga acaaagcaaa cccagttgag
      481 ctcataggcg gctaacatga agaccaaaga agcaaaaatt gtgagcaaag tggcttccag
      541 gaccgttacc ttgccgtggg gtagttccca gttttttgtg cgcgggttta atgcgtcgaa
      601 ttttcgatcc gccagccgat tgaaacccat cgccgccgtc cgcgcaccaa ccatggcaac
      661 caaaatccaa agcagacttc gtagcgttac cggatgtcta ggccatgcga gggcaaccga
      721 ggataaggcg aagggcagcg cgaacacggt atgggagaac cgcaccagac tgctgtagtc
      781 ggtgagcttg gtttttaaac cgcgcggctc attaataatt ttcgattcgg gcacgggttg
      841 cacgcttaaa tccccaactt tttccagatt gcgtcgatgt gttttttggt tttctcgtcc
      901 atgactatag cgccgggcca ctcccgctca aacccttcgc tgcgccattt gcgtgtccca
      961 tcgatgccga gctttgagcc atacccgagc atgggtgcgg cgtggtcgag aacatcgacg
     1021 ggtccgtcgg aaaagatagt gtcgcgtttg ggctcgatat gattgccgac cacccaggta
     1081 acttcggcaa gatcatgcac gttgacatcg tggtcgacga cgatgagcgt cttagtgaac
     1141 atcatctgac cgagacccca cagggcgtgc atcacttttc gagcgtgggc cggatattct
     1201 ttcttgatcg aaatgattgc taggttatga aaaaccccgt gtacgggaag attcatgtcg
     1261 acgatctctg gcagggtgac acgcaacagc ggcagaaata gcctctccgt ggccttgccg
     1321 aggtaagcgt cctccatcgg cgggcggccg acaatcgtcg tggggtagat gggatttttc
     1381 ctgtgggtga tgcaggtcac gtgaaacacc ggataatcgt cggctagcga ataaaacccg
     1441 gtatggtcgc cgaaaggtcc ctcgcgccgc agcggctctg cgggatcgac gtagccttcg
     1501 ataacgatgt ccgaattcgc tgggacttcg acatcgacgg taacgcctcg gactagctcg
     1561 acccctttct tgcgcaagaa gccggtaaaa agaatctcgt cgacctgcgg cggcagcggc
     1621 gccgtggcgg catagatcat cgccggatcg ccgcccaggc aaacggccaa ttccaatttg
     1681 ttcttgttct ccttgtgttg ttgaaagtgg cgcgcgccga ccttgtgcag gtgccaatgc
     1741 atgccggtcg ttcgccgatc aaatacctgc attcggtata gtccgacatt ccgtgtgccc
     1801 ctagcgggat ccttggagaa gaccatcggc aacgtgatga acggaccggc atcgcccggc
     1861 cagcaggtca gcactggcag caagttcaag tcgggatcgc ggatcacgac ttcgtggcaa
     1921 gccgcttctt tgacaatctt gggtggaatg ccggctaatt tgaccagtcc accgagtagc
     1981 tgtagtttat ctttaaacgt tttcggcggg cgggtttgga tgagcttggt gatctcactg
     2041 gcaatttcct cgatgtcttt gacgcccaag gccaacgcca tgcgtgtggt cgaaccgaag
     2101 gcattgatca ataccggaat ggttttgccg atgacgtttt cgaacagcaa cgccggaccg
     2161 gcggatttca tcacacgatc ggcaatttcg gtgatctcca attcggcatt caccgggtgc
     2221 gatatccgtt tcaactcgcc cgcgcgttcc aaaacctgga cgaactctgt taggttgtcg
     2281 taggccataa gaagtagttt tcgaacgtaa atgaaaagcc caaagcgtgc aaccagcgct
     2341 cagccaggcc ggtcaaaaca agcggtaaga acgcaaccat ttcctacctg ggttgtgccg
     2401 ctttgtgttg cgctggcgac actgattgcg ttctttcctg ctttgctaaa tgattttgtc
     2461 aattgggacg atgacaagat tctctacgac aatcccaatt accgcggctt gggatgggtt
     2521 cagctgaagt ggatgttttc cacgcttctc atgggacact accagcccct gacatggttg
     2581 agctttgcgc tcgactacca ggtctggggg atggcgccat ttggctacca cttgacgagt
     2641 ttgcttctgc atacggcaaa tgccgtgctg ttttactgca taagccgccg actactgggc
     2701 gcggcggtcg gaacattgac gcaaaagcac agttggcaga tcgaactgag cgcagggcta
     2761 tcggcgctgc tatttgcgat ccacccgttg cgcgtcgaat ctgtggcatg ggcgaccgag
     2821 cgccgcgatg tgctctccgg cttcttctac ttcatgactt tgtatttgcg ggctggagaa
     2881 aatttgcccc caagcattca acgacgttgg ctcggcgccg ctgcgtttac gtatctttta
     2941 tcgctgcttt cgaaagcgac ggcgatcact ttgccggtga ttttactttt actggacgtc
     3001 tatccattac gccgcttggc tggaaacccg tacgattggt tcaagcgcga gtcgcgcgaa
     3061 gtattctacg aaaaattgcc gttctttatt cttgcaatcg gtttcgccat taccgcgcta
     3121 atcgcgcaac aagttaccgg tgcgcttaag ccgctggagc agtttgatgt actttctcgt
     3181 ctgcttcagg cttgtttcgc ctatattttt tatctgtgga agacgatttg gccgttcggt
     3241 ctcgcgccca tttatgagtt gcccatcgag accggctcct ggatctgggt ttttgtcttt
     3301 tcggccgccg cgatggttgg cctaacgctc gttttttatt ctttaaagcg acattggccc
     3361 atgatctttg cttgctgggt ctactatatc gttgtcttgg cgccggtgac cggagtcgcg
     3421 cagagcggcc ctcagctagt cgcggaccgt tacagctatc tggcctgttt gggatggccg
     3481 ctgttgctcg ccggcggatt ttttcgctgg tcgtcggagc gaaacgtcca cgtgggtggt
     3541 caacggcagt tcgttgccgc cgcgatgctt ctggtcgtcg gcagtctggg gttttttaca
     3601 tggcaacaga cgctggtatg gcgctcgccg gtctcgcttt ggcaacatgg gaccaagacc
     3661 gagccgttgt cgagtattgc gcattataat ctcgggcgtg ctttagagag ggaaaataat
     3721 tcgacttcag ccattgacag ttatcgacgc gcgcttgcga tcaattccag ttactccaaa
     3781 gcgcacttca atttggcacg gttgttggct ctcgacggcc gtgaagccga ggcgatcgcg
     3841 cactaccggc gggtcatcga gattcgtccg gatcacgccg atgctcataa tgatttgggt
     3901 ttgctgctgg agatgaaagg cgaggatgcg gcggcgcttg ccgagtttcg cagggcggtg
     3961 caactcgatg ccgatcatct taaggcttcg ttcaatttgg cagagttgct ggctaaacaa
     4021 ggcgatctgg cgaacgccac cgcgcgctat gagcaggccg ggcgcctcaa tccaaacgat
     4081 tcattgattc aacttaggtg ggccattgcc ctggcgaggc aaggtcagtt ggattcggcg
     4141 acacgccatt tccgtcgcgc ggtagaactg aaaccggtcg acgccgatgc gcgggttttg
     4201 ctcgcgagat ccttggcggc gcagggaaag cacgatgaag ccgagcggca ctaccaggac
     4261 gcgctacgtt taagcagaac gggcggtaaa ggtgcggcaa acccctgagg agttcaaagt
     4321 agcactccat aaatgaagag tctcagtcgc acatcgaacc agcgctctcg ccattcgaca
     4381 ccgccagcca acgtcgcagc cgactcgagt tggatccaat gggtaatacc tttgctgctc
     4441 gcgttggcag catttgctgt gttttcctct gcactgtcca atcaattcgt cgcctgggac
     4501 gacgacctga tgttggtcga taatccaaat taccgaggtt tgggatggcc gcagctgcgc
     4561 tggatgttta cgacatttca cgccggccat tatcagcctc tcagttggat caccctggga
     4621 ctcgactact tgctctgggg tatcgatccc tttggctatc atctaaccaa cttagtcttg
     4681 catgccgcta atacggtgat tttttatttt gtctgccgcc agctcttcac caggattttt
     4741 gttgcggcac gtgaggcacg ctgggaactc gattttagcg ccgccctggc gactattttt
     4801 tttgcccttc atccgatgcg cgtcgagtcg gtggtttggg cgaccgagcg ccgggacgtt
     4861 ctttccgggg gcttttttct tgccgcgatt tattgctatc tgcgcgcgca acaagcggcg
     4921 gccttgccat cgatagcgcg caatcgctgg cttggtctcg ccgttgccgc tcaactgctg
     4981 tctttgatgg ccaaggccac aggtatcacg ctgccattag tgttggtcct cctagacatc
     5041 taccccttgc agcgtttgcc cgcttcagtg cgaacctggt tcactgcgga ctcacggcgt
     5101 gtgctcgttg aaaaattacc gtttttgctg ctctccctaa ttttcggggt ggtggcatta
     5161 tgggcgcagc aggcaagcgg cgcgctgcgc ccggtacagc tatatttttt ttcctatcgc
     5221 ttgggccaag cattctatgg catctgcttt tatgtttgga agacgctgtt gccgatcggt
     5281 ttatcgccgc tatacgagct gccatatgac ttcgaggcct ggaccggggt tttttttgcg
     5341 tgcgccggca ttacaacgct ggtcagtttg ctggctttct ttcgacgccg acgctggccc
     5401 tggctcgccg catgctgggc gtactatgtg attgtactcg cgccggtgtt aggtgtggcg
     5461 cagagcggct cgcaactagt tgccgatcgc tatagttact tgtcttgttt aagttgggcg
     5521 gcgctattgg ccggactgag tttctacgtt ctgcaaagtg ccaaaccggg acgcatggcg
     5581 attgcggtcg ttgggatatt ggtgaccggc gttggcttag gattattgac ccgcgcgcaa
     5641 gtgggtgctt ggcgcgatac ggagtcgttg tggacccata ctttgcgagt tacccccaat
     5701 accgccattg ccaattacaa tctgggcaaa ttttacgaga ccaacggcag cgcccaaaaa
     5761 agcatggagc tttaccgccg cgctgtaagc atcaatccaa ctcatacaga agcgcactac
     5821 aacttggccc ggctcctggg ccaacgaggt caggtggacg aagcaatcgc gcactaccgc
     5881 gaagctctaa aattcaaacc gaatgacgtc gagacataca acaacttggg ccagctgctg
     5941 gcgctacacg gagatttgcc cgccgccttg gctgcgtacc aaaaagcact ggcgtttgat
     6001 ccgcagttcg ccaagactta tttcaatcgg ggcaggcttt tcgtgcgcag cggggaattt
     6061 gacaaagcaa tcgatagttt tcaacgcgct ctcgatctta cgccggacac tgcggagatc
     6121 catgccggcc tgggcgacgc ctacgctcgt gccggaaacg tcgatcaggc gattattcat
     6181 ctggatgcgg cgattaaact aaaaccccag ctgccgggtg ttcaagccgt gttggcccag
     6241 ctgcttgagg tccgcgcgcg cagagttgcg ggtgggaagc ggtagccaac ggcggttgtt
     6301 ccgtcgacct gactacatac ccttttaagt cgaggggcaa atcatcggct ggatcatcgg
     6361 cacatcagtg atcgtgcgat gcgagtgtta cgttttgaga gataggatcg ttgagcgtca
     6421 gtaataagat ttgtcatgtt ccatggctcg caaagaccga actaaagcgc cgtcgagatt
     6481 gccaaacgac gcggcaagcg cgatcaaggt agccacaaaa tccaccgatt tgtcaccatc
     6541 gaagctgctg ccgatcgcgg ctctcattag tttcattgga gtgttcagtt ttatttcgat
     6601 tggcgctatc gcgcgacttt cgcctaccgt tgacgagccg gttcatttgc tttcggggta
     6661 cgctgccttg aagtggagcg actaccgagc caaccctgaa catccaccgt tggctaaact
     6721 ttgggcggcg ctgccattgt tggcgttcga tatcaaagat ccacggccgt cggcgatggc
     6781 ctgggatatt attccaatca ctcgaccaca taccacgcat acagggtcag tggcggcaaa
     6841 attattgttc gttgacaacg atggcgagac actttttttc tgggcgaaag tgcaaatggt
     6901 tctgctcgct ctgctactcg gcgggattat ctatctttgg agcagacgac tgttcggcat
     6961 cgaagccgcg acggcatctc tgtttttatt cagcctagac ccgaacatca tagcccatag
     7021 tcagatcgtt catacggatg ttgcttttac agcgttattt tttaccggca gttatttttt
     7081 ttggcgcgcg ttggagaggc tcacgtggtc tagacttgcg ctttcggcat gcttcttcgg
     7141 cttggcggca agcacgaaat acgcctatct ggccatgctc gcagtttggg gcgcagtgat
     7201 cgttctcaga gcgttctccg ctcagccact ggagtgcgcc ttgggcgcgc ctcgtgtggt
     7261 ggttggccga cggcaaaaag ccgtgctctt gtgcggcatc attttttgct cgctcatcgc
     7321 tgcttattgc ttgatttggg cggcgtacgg attccgtttc gacgcgttgc cgggtaatgg
     7381 cagatcgtta ccgatggccg acgaattgaa aacattgcct cgtttgagcg gtctaatttc
     7441 attgctgaca cagtttcatc tttttccgga agcttggatc tatggtcaac tgttcgtctt
     7501 gaataatttg caacgcgaag cttaccttct cggcgcgtat tcggatcatg gattttggct
     7561 gtacttcccg atcgcgtttg cggttaaaac accactgcca acgttgatac tattgctggt
     7621 tacgctgatt ttctggggaa tgaagaaact cgaccgcgcc aaagtcaatt ttctattggt
     7681 ctgcctggcg gtctatttct tgttagccat cttgtcacgc ttgagtattg gtattcgcca
     7741 tattttgcca atttatccat ttctgttcgt cctggtcggc ggtacggtgg ggcaactctg
     7801 gcgagcccga accgccgcca atcgcggcgc aatcttgtta ttggggttat ggtctgtgtg
     7861 gtcgacgata aacatctatc cgcattttct tgcctatttt aatgagttgg caggcgggcc
     7921 gcgcaatggc cacaaagttt tgctcgattc aaacttggat tggggccaag atctcaaagg
     7981 gctgaaggat tggatggtcg aaaatcaagt aaataaaatt caatttcttt actttggttt
     8041 tcataatgag gcggagcctc gttactttgg gatcgacgcg caatttctac ctggtagttg
     8101 ggtgagcacg gctgcgatcg cggacaagta cgccgagcca tccgactatc tcgcaatcag
     8161 cgctaatcac ctgtttggcc gacattatat tcgcggcgat cgacgggttg atttccttaa
     8221 accgttttta tccttgagcc cggcggcgat cattggtcat tcgatttacg tttaccaaat
     8281 tagccaagca atcggggcat tacgcgaaac tgttaagaaa agtcctgagt cagcgcaggt
     8341 tcatgccgat ctcggtggtt tgctggaaaa tcaaggaaac acttttgacg ccgagaaaca
     8401 ttaccgccaa gcggtccaac aaaaacatcc accgactaag gcgctgtata atctcggcat
     8461 gatcttagca aagcaaaacc atctcgacga agctattgag tttttgcagc gagccagtgc
     8521 ggctagccct ggggatgaag acattcgcta tgacttagcg atgacgctag cagttcgagg
     8581 cgatcatgag cgtgcgatcg cagaattgcg cgagaccatt cgcatcgaac cgctctacac
     8641 caaggcgttt tataacctgg ccgttttgtt ggctcgtcaa ggacaaagcc gcgaagccat
     8701 agaatccctg cgcgcggcga taaatgccga cccgttattc gctaaggcgc actaccaact
     8761 tggcgtcatg cttgcccaaa ccgccgccca tgaagaatcg atacgaagtt accgccgggc
     8821 actccagatc gagcccacta tggcggaagc gcaccaaggt ttgggcaagt tgctggcgct
     8881 gacaggtcga actgacgagg gagtcgagca tctcaacgag gctatgcgac tgatcaagtt
     8941 gcggccagcc gcgcgttgat ccactgacgg tcgcgtcagt actcattctt ctggtgtgta
     9001 aattatcatt tctaagttgt aggcactctg agataaactt ctgcatgtcc ttcgattcca
     9061 ctcgagattg agtctccaaa caatgaatag acggtttcgt acagcgaaaa aaggccgaga
     9121 tgatccgcca gcccagggag cgctcaccag tcggcgccgg tccggccgat tgtcgatgtt
     9181 ttggttggtt gttggcgcgg cgttttttct aaggctggct tacctttggc aaatcgacgc
     9241 ggtgccgctt ttttataacc tggccggcga tggccgtaac tacgacgaat gggcacagcg
     9301 catcgctgcc ggcgattggt tagggcaggg ggttttttac caggcgcctt tgtatcctta
     9361 ttttctcggg gtgatggaat tttttgtcgg tcacaatctt tggctggttc gtttcctgca
     9421 aatcgttctt ggcgcgattt cttgcgggct gatttttcgc gttggtgaac ggctgttttc
     9481 tcgcacgtcc ggtgttgccg cgggcttggt tttggcgttt tattcgccgg cgatctttta
     9541 tgacggcttg attgaaaaaa gcattctcga ccttacattg ctcagcctcg ccttgtttgt
     9601 tctgctggtt gtgtccgaca atcgctgggc taaatggctc gcttgcggag ggctgctcgg
     9661 tcttctcggt ttatcgcgtg agaatgcctt gatcttggtg ccggttgtcg ctgcatggat
     9721 cattttccac aatcgcgagc gctcggtcgc tctgcgggcg cgttggctcg gtctgttttt
     9781 cgccggtgtc tcgttggttc tattgcccat cggcttgcgc aacttgctgg tcggcggcga
     9841 gtttaagctg acaacttcgc aattcggcgc caacttttac atcggtaaca atccggtagc
     9901 tgacgggact tatggctcga ttcgaaacga gattaaagag acacaactgg aaggaccgga
     9961 cgctaagcga ctggccgagc gcgcgctagg tcagtcgctg actccaggac aggtgtcgag
    10021 ctattggtac caccgtgcgc tcgagtacat ctctgggcag ccggtgcagt ggctgaggct
    10081 cgtggggttc aaatggcttt tagtatggaa cgcgcgtgag gtcgaggact ccgacgattt
    10141 ttacatttat aagaattggt cctggctgct cgcgctactc gcgtgggtca gccattttgg
    10201 cattttagcg ccacttgcga gcgtcggcgt cttgctgacg ctgcgccagt ggcgccagct
    10261 ctggctgctt tatgccatga tcgttgcgtt gacagccagt gtcgcagtgt tctat
//
LOCUS       DATLCH010000163         7287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7675373, whole genome shotgun sequence.
ACCESSION   DATLCH010000163 DATLCH010000000
VERSION     DATLCH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7675373"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..563)
                     /gene="moaC"
                     /locus_tag="VMZ02_04765"
     CDS             complement(84..563)
                     /gene="moaC"
                     /locus_tag="VMZ02_04765"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HUR71275.1"
                     /translation="MAQLSHLDEHGRARMVDVSEKEVTSRIATACGTIHMLPETLALI
                     LDDKIEKGDVLSVARVAAIMAAKKTAELIPMCHPLNITSVHVELTPASDPARIDIEAS
                     VRVSGKTGVEMEAMTAVSIAGLTIYDMCKAVDREMIIGEIRLVKKSGGKSGTFIRTE"
     gene            611..1552
                     /gene="trxB"
                     /locus_tag="VMZ02_04770"
     CDS             611..1552
                     /gene="trxB"
                     /locus_tag="VMZ02_04770"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HUR71276.1"
                     /translation="MDKKSHYRVIILGSGAAGLTAAIYTARANLQPLVIEGSQPGGQL
                     TITTDVENFPGFPKGIMGPELMEEFKKQALRFGTEALFAEVTAVDLKQRPFKITASKE
                     IYTCDTLIVATGATAKLLGLQSEMKLMGHGVSACATCDGFFFKDKELVVVGGGDTAME
                     EATFLTKFASKVTVVHRRDKLRASKIMQDRAERNPKIFFVWDTTVEEVYGDPKAGGVK
                     GVKLRNLKNGKTEDFKCDGLFIAIGHEPNSKLFVGQLDLDPTGYILTHDGSKTNIPGV
                     FASGDVQDHIYRQAITAAGSGCMAAIDAERFLESQKE"
     gene            complement(1729..2100)
                     /locus_tag="VMZ02_04775"
     CDS             complement(1729..2100)
                     /locus_tag="VMZ02_04775"
                     /inference="COORDINATES: protein motif:HMM:NF036460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HUR71277.1"
                     /translation="MSKRVVQFAILLIALLISPSILRAQTVPVAEKQKIDALIEHVGE
                     LKDAKFIRNGSSYEPAAAVRFLRGKWNANKAEVKTARDFIDKVATKSGTSGKPYLMRY
                     SDGKEIPSREFLLAELKKIES"
     gene            complement(2169..2801)
                     /locus_tag="VMZ02_04780"
     CDS             complement(2169..2801)
                     /locus_tag="VMZ02_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HUR71278.1"
                     /translation="MNIHHIDNKKTAVLFFDMLNVYFHGIPEEKQKALKPVIDNAVRL
                     MNGARQAGILIYYAMANHRADGHSRYTAITDTDMRLRPWPNDDCNPTVHGASEGSWEQ
                     KVIDPIAPQPQDYIIPKYRWSTFHQTYFDLALRSRGIDTIIISGGAVDVGVASTVYSA
                     RDHDYNIIVARDACSNSHEDSMKALMDTVFPRLARVRTTDQVLDMIRKGG"
     gene            complement(2846..3766)
                     /locus_tag="VMZ02_04785"
     CDS             complement(2846..3766)
                     /locus_tag="VMZ02_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71279.1"
                     /translation="MSTEIQAILNPTGLVHGHTEVRFLNDTIPVLTKVLGLDLLERRD
                     REAVLKHPNTGWKLIVHEGGAEVKDKPERNHYGVRVANNEQVDHAYRYLLANKETLGL
                     TKVVKRKERAGSYSMFFVEPGGNYWEIESYENRHKAGLPYDVAYPWKTVLTEERLPGC
                     GYVPQAMTHGTIECTDLQASKKFYQQGLGLDVITHVPTIEPHDVKHPSTPWYVVSLEV
                     PSKNKHYLTPLQRYTVAVAAPAALAEAHREFTERKEEFNLTAIEAVQDTAAGQSFQIC
                     DLDGNWWEVAYISHWRCGNHAPGVLLNWSD"
     gene            complement(3815..4864)
                     /locus_tag="VMZ02_04790"
     CDS             complement(3815..4864)
                     /locus_tag="VMZ02_04790"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71280.1"
                     /translation="MKQSMLLRFVASVISVQFVLSLLASTALANPYLPKPGEAPLRAR
                     VGTCSITGTIIHLSTALDHGIFEKYGLRMEHIAFRGSSVTLAALGADEIQFLTCAASA
                     TLPGLSTGVEAKYIGSQLVGLPWFMLGRKEIRTPLDLKGRAIGITRVGDLTHRLALEV
                     LKKFNLSPQEVKLQPVGGSGQLEPYNAMAAGVVQAVLVSPPLEVRGKKDGFNVVYNLK
                     DMGIPFVYSSLHANNKTIATRPLAVQRYVAAMAETLHFTEMNPAKAKASLGKTLKLND
                     PEALQSAYDAFAKTIANRRLTVPMSSIEEMIEQAREAGTQTRRKAVDIVDNRFAEDLA
                     KSGFLTELWGQEPPK"
     gene            complement(4885..5829)
                     /locus_tag="VMZ02_04795"
     CDS             complement(4885..5829)
                     /locus_tag="VMZ02_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUR71281.1"
                     /translation="MKLVTFFYDEQERIGAIDQSGNVVDLINAYASYLRIDRAAEAAR
                     LAAAALGRDMIEFLKRGDKAIDAARIALKHAVTDGGADNVVERHRVRLMAPVPRPGAL
                     VSAGKNFSDHVAEMSSKTGPVAPVAFLKLPGSVIGPEDDISHPPEVKNLDYEVELAVI
                     IGKPCVDVGLHEAMDYVAGYASFNDISARDIIRRENKSGIHLMGKSFPGFAPMGPYLV
                     TSDEVPDPQNLKLWLRVNGETRQDSNLTYMIFKIRDMIAYWSQMGLNPGDVLTTGTPR
                     GVAAGRKPDQVPWWLKPGDVVEAEVEGLGCLRNRVIAE"
     gene            complement(5826..6809)
                     /locus_tag="VMZ02_04800"
     CDS             complement(5826..6809)
                     /locus_tag="VMZ02_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUR71282.1"
                     /translation="MKLYLGLSENSLPNQTRIFGELDGRLVDLQMACAAYLTQAEGDK
                     ANAYELASYYFPETLAGFLEWGEPAQKLLDQVMSFVTKSGVRELRGPGGEKLAYDSTD
                     VRVLPPLQNPQKSFVIGFSDRARTEALPPAEIPTGYYKLPQTFVTTGAPVIWPKFSQE
                     LDADGGIAIVIGKAGKRIAPEKAWDHVAGAMLIIDITARDINKREGLTTNNLLGKNFP
                     SSTCVAPALLLGKDKQNLLALEVELALDGVVKQKFALRDCVFTVEQIIAHWSVLGIKP
                     GDWLAIGASMALQGDRLQNPVSLKIGSTIRCASKTIGALSHQVVAAGGLRR"
     gene            complement(6891..>7287)
                     /locus_tag="VMZ02_04805"
     CDS             complement(6891..>7287)
                     /locus_tag="VMZ02_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71283.1"
                     /translation="KHVMSVWMQSAPYNLRGASWNDQRDALGDTVVNMLETYAPGFTS
                     LILHRQILTPLDLQETYGLTEGHPYHAEMALDQVFFMRPVPGWARYRTPIDHLYLCGS
                     GTHPGGGITGLPGYYAAKELLKDFKTKKS"
BASE COUNT         1522 a         1908 c         2141 g         1716 t
ORIGIN      
        1 acgattaaca caggagtgat ggagtaccgg agtgttggag tgctggattc cggatggccg
       61 aagctccatt tctccacacg agatcactcc gttctgatga aggtgccgct tttgccgccg
      121 ctctttttaa ccaagcggat ctcgccgatg atcatctcgc ggtcgacggc tttgcacata
      181 tcatagattg tcaaacccgc gatcgatacg gcggtcatcg cttccatctc cacaccggtt
      241 ttgccgctga cgcgcaccga ggcttcgatg tcgatgcggg ccggatcact cgccggcgtc
      301 agctcgacgt gcacggaggt gatgttgagc ggatggcaca tgggtatgag ttcggcagtt
      361 ttcttcgcgg ccataatggc ggcgacgcga gcgactgata aaacgtcgcc cttctcgatc
      421 ttgtcgtcga gaatgagtgc caaagtttcc ggcagcatgt ggatggtgcc gcacgcggtg
      481 gcgatacggc tggtgacttc cttttcgctc acatcgacca tgcgcgcgcg cccgtgttcg
      541 tccaagtgac tcagttgtgc catagcctta gtttcttttt ccactaggct ttttatgtta
      601 cagaaaccgg atggataaga aaagtcatta tcgtgtgatc attcttggtt ccggcgccgc
      661 tggtctcacc gcggcgatct acactgcgcg cgcaaatctt caaccgctcg ttatcgaagg
      721 ctcgcagcct ggcgggcaac tgacgattac cactgatgtg gagaattttc ccggctttcc
      781 caagggaatt atgggcccgg agctgatgga ggagttcaag aagcaagcgt tgcgtttcgg
      841 caccgaggca cttttcgctg aagtcactgc cgtcgatttg aaacagcggc cattcaaaat
      901 taccgcgagt aaggaaattt acacatgcga cacattgatt gtcgccaccg gcgcaacggc
      961 gaagctcttg ggtttacaat ctgaaatgaa actcatgggc cacggcgtct cggcgtgcgc
     1021 cacttgtgat gggttcttct tcaaagacaa agagcttgtg gtggtcggcg gcggtgacac
     1081 ggcgatggaa gaggcgacgt ttctgacgaa attcgcgagc aaggtgacgg tcgttcaccg
     1141 ccgcgataaa ctgcgtgcgt cgaaaattat gcaggatcgc gccgagagaa atcctaagat
     1201 tttctttgtc tgggacacca cggtggaaga agtttatggc gatccaaaag ctggcggtgt
     1261 aaagggcgtc aagctgcgaa acctaaaaaa cggtaagacg gaagatttca aatgcgacgg
     1321 cttgttcatc gctatcggac acgagcccaa ttcgaaattg ttcgttggtc aactagatct
     1381 tgatccgacg ggttacatcc ttacccacga cggctccaag accaatattc ccggggtctt
     1441 cgcctctggc gacgtgcaag atcacattta ccgccaggcg atcactgccg cgggcagcgg
     1501 ttgcatggcg gcaatcgatg ccgagcggtt tttggagagc caaaaagagt aatcgctaaa
     1561 cgacttactc gtcagttcgc cacgaacgcc tctaactcac cagagaaacc gtgaacctgc
     1621 gtaagagaat tgatggccga ttgttcgtcg tgcaggagca gcgcggcggg tatcgggaac
     1681 tacgctttta acagccagac gtgagcgggg ctgttggacg agggcgaatt aagattctat
     1741 ttttttgagc tctgccagga gaaactcacg gctggggatt tctttgccgt cgctgtagcg
     1801 cattagatag ggcttgcccg aagtgcccga cttggtggcg actttgtcga taaaatcccg
     1861 cgcagttttg acttccgcct tgttggcgtt ccatttgcct cgcaaaaaac gcacggcggc
     1921 cgccggctca taactggagc cgttacgaat gaattttgcg tctttcagct ctccgacgtg
     1981 ctcaatcagc gcgtcgatct tttgcttctc cgctacaggc acggtctggg cccgcaggat
     2041 ggatggcgaa atcagcaacg cgatcagcag aattgcaaat tggactacgc gtttagacat
     2101 tggcgcggct cccatttaac aattagacgc ccaacctgca tgaataatag cgcgaatgtt
     2161 cgggtgactt aacccccttt gcggatcata tccagtactt gatcggttgt tctgacccgg
     2221 gccaagcggg gaaagaccgt gtccatgagt gctttcatgg agtcttcgtg ggagttgctg
     2281 caggcgtcgc gcgcgacaat gatgttgtag tcgtggtcgc gggccgagta aacggtcgag
     2341 gcgacgccta cgtcgacggc gccgccggaa ataatgatcg tgtcgatgcc gcgggaacgt
     2401 agcgccaagt cgaaataggt ctggtggaat gtgctccagc ggtacttcgg aatgatgtaa
     2461 tcctgaggct gcggcgcaat cggatcgatg actttttgtt cccacgaccc ttcgctggcg
     2521 ccgtgcactg tcggattgca atcgtcgttg ggccaggggc gcaggcgcat gtctgtatcg
     2581 gtgatcgccg tgtagcggct atggccgtcg gctcggtgat tggccatggc gtaatatatt
     2641 aggataccgg cttggcgcgc accgttcatc aaacgcacgg cgttgtcgat cacgggtttc
     2701 aaggcttttt gtttttcctc cggaatgccg tggaagtaga cgttgagcat gtcgaagaac
     2761 agcacggcgg tttttttatt gtcgatgtgg tggatgttca tgattggcag ctcctaagac
     2821 attggcacta atcgttacta ggttgttaat cgctccagtt caataaaacc ccaggagcgt
     2881 gattgccgca gcgccaatgt gaaatgtaag cgacttccca ccagttgccg tcgaggtcgc
     2941 agatctgaaa cgactggccg gcggccgtgt cttggacggc ttcgatggcg gtcagattaa
     3001 actcctcctt gcgctcggtg aattctcgat gagcttccgc aagcgctgct ggcgctgcga
     3061 cggcaacggt gtagcgctgt agtggagtta aataatgttt gtttttcgac ggtacttcca
     3121 aactgacgac gtaccagggc gtgcttggat gcttcacatc gtgcggttcg atggtcggca
     3181 catgggtgat gacgtcgagg cccaggcctt gctgataaaa cttcttcgaa gcctgcaagt
     3241 ccgtgcattc aatggtgcca tgtgtcatcg cctgcggaac gtagccgcat ccgggcaagc
     3301 gttcttcggt gagaaccgtc ttccacggat atgcgacgtc gtaaggtagg ccggctttgt
     3361 ggcgattttc ataggactcg atttcccaat agttgccgcc gggctcgaca aaaaacatcg
     3421 aataggagcc ggcgcgctcc ttgcgcttga ccactttggt gaggccgaga gtttctttgt
     3481 tggcgagcaa atatcgatag gcgtgatcga cttgttcatt attcgcgaca cgcacgccgt
     3541 agtgattgcg ctcgggtttg tcttttactt cggcgccgcc ttcgtggacg atcagtttcc
     3601 aaccggtgtt gggatgtttc aagaccgctt cacgatcccg ccgttcgagc aggtcgagtc
     3661 caagtacttt ggtgagcacg gggatggtgt cgttgagaaa gcgcacttcg gtgtggccgt
     3721 gcaccaggcc ggtcgggttc aaaatggctt gtatctcagt gctcatgtgc actcctcaaa
     3781 cgcgcgaagc ctgagaagaa tcgtcggtgt aatatcactt cggcggttcc tggccccaca
     3841 gctcggtcaa aaatccgctt ttggcaaggt cttcggcgaa ccgattgtcg acgatatcga
     3901 cggctttccg acgggtttgc gtaccggctt cgcgggcttg ctcgatcatt tcctcaatgg
     3961 agctcattgg cacagtcaag cgccggttgg cgatggtttt cgcaaaggca tcgtaggcgg
     4021 attgcagcgc ttcgggatcg ttgagcttga gagttttgcc taacgacgct ttggctttcg
     4081 ctggattcat ttcggtgaaa tgaagagtct ccgccatggc cgccacatag cgctgcacgg
     4141 ccagcggtcg tgtcgcgatg gttttattgt tggcgtgcag cgaactgtag acgaacggta
     4201 tgcccatatc tttcagattg tagacaacgt tgaagccgtc ttttttgccg cgtacttcca
     4261 gtggcgggct gaccagcact gcctggacga cgcccgcggc catggcatta tagggttcca
     4321 attgaccgct accgccgacc ggttggagtt tcacttcctg gggcgacaaa ttgaatttct
     4381 tcaagacctc aagcgccagg cggtgcgtca aatcgcccac tcgggtaatg ccgatggcgc
     4441 gacctttcaa atcgagcggc gtacgaatct ctttgcggcc gagcatgaac cagggcagac
     4501 cgaccagctg agagccgata tattttgctt cgacgccggt agacaggccg ggtagcgtag
     4561 cggaagcggc gcaggtcaaa aactgaatct catcggcgcc taaagccgcc aatgtcacgc
     4621 tactgccgcg gaaggcgata tgttccatgc gcaagccgta tttctcgaat atgccgtggt
     4681 cgagcgccgt cgataggtgg atgatcgtac cggtgatcga gcaggtgccg acgcgggcgc
     4741 ggagcggcgc ttcgcccggt tttggaagat atggattggc cagcgccgtg ctagcgagca
     4801 ggctaaggac gaattggacc gatatgacgg atgcgacgaa ccgcaataac atcgattgct
     4861 tcatagtagt cgttgcaaag cgctctactc ggcgatgacg cggttgcgca gacagccgag
     4921 tccctcgact tcggcttcga cgacgtcgcc gggttttaac caccacggca cttgatcggg
     4981 ttttctgccg gcggcgacac cgcgtggtgt gccggtggtg agcacgtcgc ctggattcaa
     5041 acccatctgc gaccagtagg caatcatgtc gcgaattttg aaaatcatgt aagtgaggtt
     5101 cgaatcttgt cgcgtctcgc cattgacgcg cagccaaagt ttcagattct gcggatcggg
     5161 aacttcatcg ctggttacca agtagggtcc catgggcgcg aagccgggaa agcttttgcc
     5221 catgaggtgg atgccgctct tgttttcgcg acggataatg tcgcgggcgc tgatatcgtt
     5281 gaacgacgcg tagccggcga cgtagtccat cgcttcatga aggccgacgt cgacgcacgg
     5341 tttgccgatg atcaccgcaa gttcgacctc gtagtctaga ttcttcactt ccggcggatg
     5401 agatatgtcg tcttcggggc cgatcaccga gcccggcaat tttaaaaagg ccactggcgc
     5461 caccgggcct gtttttgacg acatctcggc aacgtgatcg gaaaagttct tgccggcgga
     5521 gactaacgcg ccgggacgtg gcactggcgc catcaggcgc acgcggtggc gctcgacgac
     5581 attgtcagcg ccgccgtcgg tgactgcgtg cttgagggct atgcgggcgg catcaatggc
     5641 tttgtcgccg cgctttaaaa attcgatcat gtcacggccg agggcggctg cggccaagcg
     5701 cgctgcctcg gcggctctat caatgcgtaa ataggaggcg taagcattaa ttaggtcgac
     5761 aacattgccg gactgatcga tggcgccgat tctttcttgc tcgtcgtaga aaaaagtaac
     5821 cagcttcacc ggcgtagtcc tcccgccgca acaacttgat gcgacagtgc gccgatagtt
     5881 tttgatgcac agcggatcgt cgaaccgatt ttaagggaca cgggattctg taagcggtcg
     5941 ccttgcaacg ccatgctggc gccgatggcg agccaatcgc ccggcttgat cccgaggacc
     6001 gaccaatggg cgatgatctg ctcgacagtg aacacgcaat cgcgcaaggc gaatttctgt
     6061 ttgacgacac cgtcgagcgc tagctccact tccaaggcga gtaggttttg cttgtccttg
     6121 ccgagcaata gggcaggggc gacacaagtg ctcgatggaa agtttttgcc aagcaaattg
     6181 ttggtggtga ggccttcgcg cttgttgatg tcgcgcgcgg tgatgtcgat gatcagcatc
     6241 gctccggcta catgatccca ggctttttcc ggcgcgatac gcttgccagc cttgccaatc
     6301 acaatggcaa tgccgccatc ggcgtcgagc tcttgggaaa atttcggcca gataaccggg
     6361 gcacccgtgg tgacaaaagt ctgcggcagt ttgtagtagc ccgtggggat ctccgccggg
     6421 ggcagcgctt cggtgcgtgc gcgatcggaa aagccgataa cgaaactctt ttgcggattc
     6481 tgtaatggag gcaggaccct tacgtcggtg ctatcatagg cgagcttctc gccaccgggg
     6541 ccgcgcaact cgcgtacgcc ggattttgta acaaaggaca tgacttgatc gagcagtttc
     6601 tgcgccggtt caccccattc gagaaaaccg gcaagagtct cgggaaaata ataactggcc
     6661 agttcgtaag cattggcctt atcgccttcg gcttgtgtca aataggcggc gcaggccatc
     6721 tgtaagtcga ccagccggcc gtccagttcg ccgaagattc tcgtttggtt cggcagggaa
     6781 ttttccgata aaccgaggta gagtttcatc aaaacataat gccttacgaa taaccgaacg
     6841 gtttaatttc ccgtctatca cgaagagcgg aattttccca tgggaccgct ttaggatttt
     6901 ttggttttaa aatctttcaa cagttctttg gcggcatagt atcccggcag gccggtgatg
     6961 ccgccgccgg ggtgagtgcc ggagccgcag agatataaat ggtcaatggg cgtacggtag
     7021 cgcgcccaac ccgggaccgg gcgcatgaag aagacttggt ccaatgccat ttcggcatga
     7081 taggggtggc cttccgtgag gccgtaagtt tcttgcaagt cgagtggcgt caatatttga
     7141 cgatgtagga tcaaacttgt aaatcccggc gcgtaagtct cgagcatatt gacaaccgtg
     7201 tcgcccaagg catcgcgctg gtcgttccaa ctcgcacccc gcaagttata cggcgccgac
     7261 tgcatccaca ccgacatgac atgcttg
//
LOCUS       DATLCH010000164         3140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5668181, whole genome shotgun sequence.
ACCESSION   DATLCH010000164 DATLCH010000000
VERSION     DATLCH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5668181"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VMZ02_04810"
     CDS             <1..496
                     /locus_tag="VMZ02_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71284.1"
                     /translation="DRDNAVWYGGLALYWGKEKAAKFMKALAGQQPTMRKGHTLIANL
                     MSSGEFPLGLVYAHRVEEMKSKGMNTIEWVALEPIVATPIVIGITKNAPSPNAAKLFV
                     DFFLSKEGQVISQKQQFRVPANPDMPPVSAKLDPKNLKIALVNRQLADSYQEYDKEYQ
                     ELFK"
     gene            complement(546..2039)
                     /locus_tag="VMZ02_04815"
     CDS             complement(546..2039)
                     /locus_tag="VMZ02_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HUR71285.1"
                     /translation="MGQATAPFQILEATIDSIHAAYKSGQLTCRQLVQTYIDRIEALD
                     KAGPAINSILAVSSTALAEADRLDDAYRKSGPIGPLHGIPVILKDQIDARGMATTLGS
                     VLFKNFYPDKDAFIVEKMRRAGAVILGKSTLGELGGGDTHGSLFGSTKNPYSLERTVG
                     GSSGGSAASVSANLAALGVGQEGLASIRRPSTWNSIVGMRTTAGVVSRAGSYSGWPQK
                     AGTLGPMARTVKDLTLLLDAIAGYDSEDPLTARGFGHIPESFTNYLDKDGLSGARIGI
                     LREPMGYNSEPDSDDFKQVGAVFDRAIGELRAGGAQLVDPIVIPKLNELLAKRAENPT
                     AGEEAFSIYYGRNGNPPFKSRAEAMAAPEFAKVFPGAKNRLKTNSDAAKYAQYLIACD
                     ELMTNVLKVMADNRLDAIVHKSVEHQPTLIRDGINPPHVNHRGAPHLNTFLIFVPTIA
                     VPAGFTRDGLPAGISFLGRPYSDGQMIRFAYAYEQATHHRRPPASAS"
     gene            2287..2823
                     /locus_tag="VMZ02_04820"
     CDS             2287..2823
                     /locus_tag="VMZ02_04820"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HUR71286.1"
                     /translation="MSFTLTDLFADLDGCAESLSLDYLTDRLSKLEISLDDVREHLQF
                     GAKTYQRNLIHQGPSYQALVLCWRSGQRSPIHDHRGSICGVRVLGGLAIETLFEQTKD
                     GWIYASGSHELGKNQVCGSEDSDIHQISNLCPQGEDLVTLHIYSPALLVMGTYSLTGN
                     ARKEFTDPVYTFSDGAGI"
BASE COUNT          689 a          874 c          876 g          701 t
ORIGIN      
        1 cgaccgcgat aacgccgtct ggtacggcgg tctggcgctc tattggggca aggaaaaagc
       61 cgcgaagttc atgaaagctt tggctggcca gcaaccgacc atgcgcaaag ggcatacgtt
      121 gattgccaat cttatgtcga gcggcgagtt tccgctcgga ctcgtctacg cacatcgggt
      181 tgaggagatg aaatcgaaag gaatgaacac catcgagtgg gttgcgctcg agccgattgt
      241 tgcaacgccg attgtcatcg gcataacgaa aaatgcgccg agcccgaacg ccgccaagtt
      301 gttcgtcgac ttcttcttgt cgaaagaagg tcaagttatc tcccagaaac agcaattcag
      361 ggtgccggca aacccggata tgcctccggt ctccgccaaa ctcgatccga aaaatctaaa
      421 aatcgctctg gtcaaccgcc agctcgcgga tagctatcaa gagtacgaca aggaatacca
      481 ggagcttttt aaatagctcg cggcaactct gcgaaccggg tgaactcttc tagatcgaac
      541 gccgattaac ttgcactggc gggcgggcgg cgatggtgcg ttgcctgctc gtaagcgtac
      601 gcgaacctga tcatttgccc gtcgctgtaa ggccgcccga gaaagctgat tccagccggc
      661 agcccgtcac gcgtgaatcc cgcgggaacg gcgatcgtcg gcacgaatat caaaaatgtg
      721 tttaggtgcg gcgcgccgcg atggttgacg tgaggcggat tgattccatc gcggatcagc
      781 gtcggctggt gttctacgga tttgtgaacg atggcgtcga gcctgttgtc tgccatcacc
      841 tttaaaacgt tggtcatgag ctcatcgcag gcgatcagat attgagcgta ttttgccgcg
      901 tcggaatttg tcttcaggcg attctttgca ccgggaaaca ctttggcaaa ctccggcgcc
      961 gccatcgcct cggcgcgaga cttaaacggc ggattgccat tgcgaccgta gtagatgctg
     1021 aacgcttcct ctcctgctgt gggattttct gcgcgcttgg ccaacaattc gttaagttta
     1081 ggaatcacga tgggatcaac cagttgggcg ccaccggctc ttagctcgcc aatcgcgcga
     1141 tcgaagaccg caccgacttg cttgaaatcg tccgaatccg gttccgaatt gtagcccatc
     1201 ggctcgcgca atatgccgat gcgcgcgccg ctcagaccgt ccttgtcgag ataattagtg
     1261 aagctctcgg gaatgtgacc gaagccgcgc gccgtcaacg ggtcctcgga gtcgtagccg
     1321 gcgatggcgt cgagcagtaa cgtcagatct ttaaccgtgc gggccatggg tccgagagtg
     1381 ccggctttct gcggccaacc ggaatagctt ccggcgcggc tgacaactcc cgcggtggta
     1441 cgcattccga cgatggagtt ccacgtcgac ggtcggcgga tcgaagccaa cccttcctgc
     1501 ccgacaccga gcgcggcgag atttgccgac acgctcgcag cagaaccgcc ggaggagccg
     1561 ccgacggtgc gctccaaaga atacggattc ttggtcgatc caaacagcga cccatgggta
     1621 tcgccgccgc caagctcgcc cagcgtcgat tttcccagta tgaccgctcc cgcgcgcctc
     1681 attttttcga cgatgaaggc gtctttgtcc ggataaaagt ttttgaacaa caccgatccc
     1741 aaggttgtcg ccatcccgcg agcatcgatc tggtccttga ggatcaccgg gattccatgc
     1801 agcggaccaa ttggaccgga ctttctataa gcatcgtcca gacgatccgc ttcggcgagc
     1861 gccgtcgaac tcacggcgag gatcgagtta atcgccggac cggctttgtc caaagcctcg
     1921 atgcggtcga tatacgtttg gaccagctgg cggcacgtca actggcccga tttataagca
     1981 gcgtgaatgc tatcgatggt tgcttcgaga atctgaaacg gcgcggttgc ttggcccacg
     2041 gttagctcct tagtttccag agaaatttat cttgccgaaa ccatatggca ggccagaaaa
     2101 gatgtctatc gctcaaacgc caaattacat tgcgctacta ccatcgcgtt ttgtctcgaa
     2161 cgacgaggac atgatcggca gtgctatttt agctctccag taaacattct tttggtccaa
     2221 cccagatcac ttttggttca cggtgctccg cgtgatacaa tatttttctc tgatggaggt
     2281 tgcctcgtga gtttcacttt gactgatttg ttcgccgatt tggatggttg cgccgaaagt
     2341 ctatcgttgg actatctgac cgatcgtttg agcaaactcg aaatctccct tgacgacgtc
     2401 cgtgagcatt tgcagttcgg cgctaagact taccagcgca acttgattca ccaggggcct
     2461 tcatatcagg cgctggtcct ctgttggcgt tccggccagc gcagcccgat tcacgaccac
     2521 cgcggctcga tctgcggcgt ccgggtcttg ggcggcttgg caatcgagac gctcttcgag
     2581 cagaccaaag acggctggat ctacgcgagc ggatcgcacg aactgggcaa aaaccaagtg
     2641 tgcgggtcgg aggacagcga catccaccag atttcgaacc tctgcccgca gggtgaagat
     2701 ctggttactc tgcacattta ttcacccgcg cttttggtca tgggaaccta ttcgcttacg
     2761 ggcaacgccc gcaaggaatt caccgatccg gtttacacat tcagcgatgg cgctggaatt
     2821 tgactcgaaa cgtagccgcc tgggtcaaca aataccctca acgaaacttg tcccgctgcc
     2881 cgaaacgcag acgtataagt ttgagttcgc gcaggtctgt ttgacggtgc tgtggcatgt
     2941 ctaacaatag cgtggatctt agcagggaca ggccgagctg ccggccccaa gcaaggagaa
     3001 tcgcggtatg aaagacgtcg aacaagagct catagcgact gcgcggccct attgggaaac
     3061 tgaagctacg atcgttcggc gttttttcaa gagcaaaccg acgcgggagg atcatatttt
     3121 ttggctcaag gcgcagctgt
//
LOCUS       DATLCH010000165         6141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7677170, whole genome shotgun sequence.
ACCESSION   DATLCH010000165 DATLCH010000000
VERSION     DATLCH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7677170"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VMZ02_04825"
     CDS             <1..575
                     /locus_tag="VMZ02_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HUR71287.1"
                     /translation="MRASVTTRHKKYAETHSGSGQSKGFDTPTGKIEIYSTAFGDAGY
                     PPIPKFMNDESANIDYPLTLTFFRDIHFCDQQHRNIPRLRRVIPEPFVEIYPTTASAN
                     GIANGDWIFLETETGKVKLQAKFNDSLHPHVVTTVYGWWQGCKELKISGHDPFSPTGA
                     NSNLLIPNGDNDPISASVAHRGQSCRVRKQ"
     gene            complement(1264..2229)
                     /locus_tag="VMZ02_04830"
     CDS             complement(1264..2229)
                     /locus_tag="VMZ02_04830"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71288.1"
                     /translation="MANPRITIAVSHYDRHVPFFDGTVQTDGIDLNVMIVGQSDRQRD
                     GEDRHERMLQDGEFDVAEVSLSSYLMAKSRGMPFTAIPVFPRRLFSHSQMWINVEAGI
                     NEPKDLIGKKVGLHSFQNTLATLSKGDLQSEFDVPWRKVHWLLSKKDNIQFEPEKGVK
                     IEQLSESANVGDMLEKGEISAMMTPHPPKPVLRGSKKIKRLFADPKAEDLKYYKKNGF
                     FPIMHVVAFRDDVLKKHPEAAASLFTAFGEAKKICREFYADPNWSWLAWGRQAFEDEE
                     RLLGDPWPLGLEKNRPNLERFVGYSLDQGLMAKKMAVEELFLPLN"
     gene            complement(2395..3717)
                     /locus_tag="VMZ02_04835"
     CDS             complement(2395..3717)
                     /locus_tag="VMZ02_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUR71289.1"
                     /translation="MAKIIDSLVDYMLAAPQVPLPAEVIQKGKSHLLDSLAAVVSGST
                     LKPGKLGLQHAREQGGRQECSVLGTNLKTTPIMAAFANGMSGHADETDDSNSQLHPGC
                     AMVPAALAVAESRNAGGEALLRAVILAYDIGFRFHQAFAPRSTTFGATFGCAAAAGTL
                     AELDATQMRYLISYAAQQASGSRAWVGDDDHIEKAFDYAAMPARNGVTAALLIASGFT
                     GNRDVLEGDQGIIKTYAPCDPALILAELGRRFTITGCLVKKYPVGSPMMETVDATLAI
                     LAKERIDTDAIEKIVVRIPESGARTVNNRKMPDVNVQFMVASILLEGTLTFDMAHDYA
                     RFQNPRVLALKEKVQLIADNEMEKSGHRFQGLVEITLKGGRLLREHVIDCRGRPENPM
                     SPEEVEKKAAWLLEPVLGKKNSDQVIDSIRRIESVSSVRDLTMLMIVT"
     gene            3881..4351
                     /locus_tag="VMZ02_04840"
     CDS             3881..4351
                     /locus_tag="VMZ02_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71290.1"
                     /translation="MNKDSCCSSRTKFFQRIIVGIAVFLAGATFTRLSAAQTRSRVIV
                     AHGVGIYSLNPYAVNTSPLTAAWGSVMESLIEPDYEKRGYRGVLAESWQLKGTRLEFK
                     LRKGVRFHDGSPFTAADVVASFKRILTDKNSLQSPNLEMSKTWRHPTRIPSSLT"
     gene            4312..4743
                     /locus_tag="VMZ02_04845"
     CDS             4312..4743
                     /locus_tag="VMZ02_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71291.1"
                     /translation="MEAPDPHTVVINLKRLDANILEDINNRVVMKQAAAEKMGEADDR
                     PIGTGPFKFTSWERSGQFVIRRNESYWGQPAKIDEVVYKAIQEDAARIAALESGQVDV
                     ISNIPPHEVARLKANPRLRVQPVQGLRPLFLVLSFRAQREI"
     gene            complement(4905..5195)
                     /locus_tag="VMZ02_04850"
     CDS             complement(4905..5195)
                     /locus_tag="VMZ02_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71292.1"
                     /translation="MKKLLLVVGLISLVSAGCSSMHSPYDLHTWCLQMGSSRLTSIGA
                     KAHDQANCDKELDEDLADRTPKILYVPRDLAMAPVLGARGLWILLGMTEPPF"
     gene            complement(5308..>6141)
                     /locus_tag="VMZ02_04855"
     CDS             complement(5308..>6141)
                     /locus_tag="VMZ02_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HUR71293.1"
                     /translation="LKRNVHLEARNHFLAVLAMEPGNREAFEALQIQVREVRQVNHTV
                     RAGESLTSIAQLYYGDRTRSEVIWENNQLPPNPKLTPGTILKIPEIPGLPLGRPDPPV
                     STAKTPPAGSTPAAGGKSESAEEELYANPALGAAREAMERGEFTLALSTVDRFLGQNP
                     RNTEAVDLRRTVLLQQSRSLMEQNKLLDAYNSAFQLVKSNPKDSTATSLLTQVRGRLV
                     QQHYSQGMQLFRDEKLPSAISEWRTVLQYEPAHDGAKRNIDQAERLLKGLQERQQKQG
                     K"
BASE COUNT         1365 a         1769 c         1636 g         1371 t
ORIGIN      
        1 gaatgcgcgc gtcagtcaca acacggcata aaaaatatgc cgaaacacat tccgggagcg
       61 gtcagtccaa aggcttcgat acaccgacag gcaagatcga aatctattcg acagcattcg
      121 gggatgccgg ctacccgccc atccccaagt tcatgaacga tgaatcagca aacatcgatt
      181 accctctcac actgacattc ttccgtgata tccacttttg cgaccaacaa caccgcaata
      241 tcccgcgcct gcgccgtgtc ataccagaac cgtttgtcga aatttatccg acaaccgcgt
      301 cggcaaatgg tatcgccaac ggcgactgga tttttttgga aacggaaacc ggcaaagtga
      361 aactccaagc gaaattcaac gactcgctcc acccccatgt cgtcaccacc gtctacggct
      421 ggtggcaggg ttgcaaggaa ttgaaaatta gcggccatga cccgttcagt ccgactggcg
      481 cgaacagcaa tctcttgatt cccaatggcg acaacgatcc aatcagcgca tcggtcgcgc
      541 atcgtggaca gtcatgtcga gtgagaaaac aataaatcaa gcgacgccaa accactgcac
      601 gtctattggt aatatcccaa ccaggtagat tggcatcacc gcggatatct actgattgat
      661 ggcaaggtta aagtcgagca actatgatct tcagggtctg tactatactg agcagtataa
      721 tcaatcgttg ggcgtcgttg catccagcgc tgcgtaggga gcaaacacga tgaaagtcaa
      781 tggttcatgt cactgtgggt tgattcgctt cactgcaaac gctgatcaga gcaaggtaag
      841 agtctgccat tgcacagact gtcaaaaact cactggctcc gcattccggg tcgtcgttcc
      901 agccccaatg gagtctctcg ttcttcacgg tgagccaaag cgctacgtta aggtcgccga
      961 tagtggggcc aagcgcacac aggcgttctg ccccgaatgc ggatcgccgg tgttttccgt
     1021 gtcactagag aacccaacgc aaatcagcct gcgcgtcgga ttactcgacg aacgaagcca
     1081 gctcatgccg tacctccaaa tttggaagcg gtctgcactt tcttgggtcg atgaccttgc
     1141 atcagtgcaa gcatgcgaac agcaagacgc tcacagtccg cgatgaatct gccattcagt
     1201 gcgctcgcat cgcagaaatg acgcccaacc atggcgggcg ttaggcggaa cattaccgga
     1261 acattagttg agcggcaaaa acaactcctc caccgccatc ttcttcgcca tcaatccttg
     1321 atcgagcgaa tagccaacga agcgctccag gttcgggcga tttttttcta gcccaagcgg
     1381 ccagggatca cctaacaacc tttcctcatc ttcgaacgcc tgcctgcccc acgcgagcca
     1441 cgaccagttg ggatcggcgt agaactcgcg gcagattttc ttagcttcgc cgaatgccgt
     1501 gaacagactg gcggcggctt cgggatgttt tttcaggacg tcgtctctga aggcgacgac
     1561 atgcatgatc gggaaaaagc cgttcttttt gtagtacttc aaatcttccg ctttgggatc
     1621 ggcgaacagc cgtttgattt tcttcgagcc gcgcaatacc ggcttcggcg gatgcggcgt
     1681 catcatcgcg ctgatctcgc ctttttccaa catatccccg acattggcgc tctctgaaag
     1741 ctgttcgatc ttgacgccct tctccggctc gaactgaata ttgtctttct tgctgagcag
     1801 ccagtgaacc ttgcgccagg gcacgtcgaa ttcgctttgc aagtcgccct tggacaatgt
     1861 cgccagggta ttttgaaaac tatgcaagcc gacttttttg ccgatcaaat ctttcggttc
     1921 gttgataccc gcctcgacgt tgatccacat ctgcgagtgg ctgaacaacc ggcgcggaaa
     1981 caccgggatc gccgtgaacg gcatgccgcg gctcttggcc atcagataag atgacagcga
     2041 cacctcggca acgtcgaatt cgccgtcttg cagcatacgc tcatggcggt cctcgccatc
     2101 gcgctggcga tccgattgac cgacgatcat aacgttcaag tcgatgccgt cggtttgcac
     2161 ggtgccgtca aagaacggca catgccggtc gtagtgcgag actgcgatgg tgattctggg
     2221 attggccatg ggtttctccg atcgtgaatt tatttgatta agttgtttcg atcagtctga
     2281 tatagctccg aaattgtttc cctcgactga aaggacaaag gaaggggtca tcttgaattt
     2341 cctccagcac cccatctcag tcttcgcccg ccaaggggaa gaagtcggat cggctcacgt
     2401 cacaatcatt agcattgtta gatcacgaac actgctcacg gactcgatgc gccgtatcga
     2461 atcgatgact tggtcggaat tttttttgcc gagcaccggc tccagcagcc aggccgcttt
     2521 cttttcgact tcttcgggac tcatgggatt ctccggacga ccgcggcagt cgatcacgtg
     2581 ctcgcgcaat aaacggccgc ctttgagcgt gatctcgact aagccctgga aacgatgacc
     2641 cgacttttcc atctcgttgt cggcaatcag ttgcactttt tccttgagcg ccaggaccct
     2701 gggattctga aaacgcgcgt agtcgtgggc catatcgaag gttagcgtgc cttctagcag
     2761 gatcgaagcg accataaatt gcacattgac gtcgggcatt tttcggttgt taaccgtacg
     2821 tgcgccactc tcgggaatgc gcacgacgat cttttcgatg gcgtcggtat cgatccgttc
     2881 cttagcaagg attgccagcg tcgcatcgac tgtctccatc atcggcgaac cgactggata
     2941 ctttttcacg agacagccgg tgatggtaaa tcgcctaccg agctcggcca agatcagagc
     3001 cggatcacac ggcgcgtaag ttttgatgat gccttgatca ccctccagca cgtcacgatt
     3061 gccggtgaag cctgacgcaa ttaaaagagc ggctgtgacg ccgttgcgcg cgggcatggc
     3121 cgcgtagtcg aaggcttttt cgatgtgatc gtcatcgccg acccaagcgc gcgatcccga
     3181 agcctgttgc gcggcgtagg aaatcagata gcgcatttgc gtggcatcca actcagccaa
     3241 cgtgccggcc gcagcggcgc aaccgaaggt agcgccaaac gtcgtcgaac gcggcgcaaa
     3301 agcttgatga aaacgaaagc cgatatcgta agcgagaatc accgcgcgca acaaagcctc
     3361 accgccggca ttgcgcgact ccgcgacggc gagcgcagcc ggcaccatcg cgcagccggg
     3421 atggagctgg ctattggaat cgtcggtctc gtcggcgtgt cccgacatgc cattggcaaa
     3481 ggccgccatg atcggcgtcg tttttagatt ggtgccgagc acgctgcact cttgtcggcc
     3541 gccctgctcg cgtgcatgtt gcaggccaag cttgccgggc tttaacgtcg accccgacac
     3601 cacagctgcc agcgaatcta aaaggtggct tttacctttt tgaatcactt ctgccggcaa
     3661 tggaacctgt ggcgccgcta acatgtagtc gaccagagaa tcgatgattt tcgccatagc
     3721 ccctcccgat ttcgaatccc tgactactga actgcagtcg agctgtcaag ataaagttga
     3781 cagtagtcta cggcctgggt tatgcgtggc gagagattgc agcctgcact ctaacggcgt
     3841 cgaattacca ctgtggtcat ccttccaaac ggaggtttcg atgaacaaag actcgtgctg
     3901 ctctagccga acaaagtttt tccagcgcat catcgtcggc atcgccgttt ttctcgccgg
     3961 tgcgacgttt acccggttgt ccgccgctca aacgcgcagc cgcgttatcg tcgctcacgg
     4021 ggtaggcatc tattcgttga atccttatgc ggtcaacacc tcaccgctca cagccgcctg
     4081 gggctcggtc atggaatcac tcatcgagcc cgactacgag aagcgcggct atcgcggcgt
     4141 gttggccgag tcttggcagc tcaaaggaac gcggctcgaa tttaaactgc gcaagggcgt
     4201 gcgctttcac gacggttcgc cattcaccgc tgccgacgtg gtcgcatcgt tcaaacgcat
     4261 cctcaccgat aagaacagtt tgcaatcgcc taatctggaa atgtcaaaga catggaggca
     4321 cccgacccgc ataccgtcgt cattaacctg aagcggctcg atgccaacat tctcgaggac
     4381 atcaacaatc gggtcgtcat gaaacaagcg gcggcggaaa aaatgggcga ggccgacgac
     4441 cgaccgatcg gcaccgggcc gttcaaattc accagttggg agcgcagcgg ccaattcgtc
     4501 atccgccgca acgagagcta ttggggccag ccggcaaaga tcgacgaagt cgtctacaaa
     4561 gcgatccaag aagacgccgc gcgcatcgcc gctcttgaat ccggccaggt cgacgtcatc
     4621 agcaacatcc cgccgcatga agtcgcacgt ttgaaagcca atccgcggct gcgcgttcag
     4681 ccggtgcaag gactgcggcc gctctttctc gtgctgtcat ttcgagcgca gcgagaaatc
     4741 taatccaccc tcactcacct gtcggacgtg accgcgggcc gttgtctgtc gcaaagatcg
     4801 caacgacgtg aatcttacct gcgggtggaa ctcttgcaac ggatcctatc gaccaagcag
     4861 acccaaccgg tgctcattct tgacggcgcg attctaagat cgcctcaaaa cggtggttcg
     4921 gtcataccga gcaaaatcca caaccctcta gcgccgagca caggcgccat ggctaggtct
     4981 cttggaacgt ataagatctt cggggtgcgg tcggccaggt cctcgtctaa ctccttatcg
     5041 cagttggctt ggtcgtgagc ttttgcgccg atcgatgtca gccgagagct tcccatctgc
     5101 aggcaccatg tatgcaggtc atacggggaa tgcatcgatg agcaaccagc gctcaccaac
     5161 gaaattaagc caaccaccag taaaagcttt ttcattacgt gctgtgtcct ttctccaaat
     5221 cgccatggca gtcggtgcgg tatgttacgc gactgggcgc gacgattatc aatcgaattg
     5281 actgacgata caaggcgatt tctcgtccta cttgccctgc ttctgctgcc gctcctgcaa
     5341 acctttgagc agccgttcgg cttgatcgat gtttcgcttg gcgccgtcat gggcaggttc
     5401 gtactgtaag acggtgcgcc actcagaaat ggcagacgga agtttttcgt cgcggaaaag
     5461 ctgcataccc tggctgtagt gctgctgcac cagacggccg cgcacttggg tcaacagcga
     5521 cgtcgcagtg gagtctttag gattagactt aaccagctgg aaagccgaat tgtaggcgtc
     5581 gagcaatttg ttctgttcca tcagacttcg gctctgctgc agtaagaccg tgcggcgcag
     5641 atctacagct tcggtgttgc gcggattctg gccgagaaat cggtcgacgg tcgataacgc
     5701 caaagtgaat tcgccgcgct ccatcgcttc ccgcgccgcg ccgagcgccg ggttggcgta
     5761 caattcctct tcagccgact ccgatttgcc accggccgca ggtgtactgc ccgctggcgg
     5821 agttttcgcc gtcgacaccg gcggatcggg ccgtcccagc ggcaagccgg gaatctcggg
     5881 aattttcaaa atagttccag gcgtcagttt cggattgggc ggcagttgat tgttttccca
     5941 aatgacttcc gatcgagtgc gatcgccgta atataattgg gcgatcgatg tcagggactc
     6001 gccggcgcgc accgtgtgat tgacttggcg aacttcgcgt acttgtatct gcaaggcttc
     6061 aaaagcctcg cggttgccgg gctccatggc gagcaccgca agaaaatggt tgcgcgcttc
     6121 gagatggacg ttgcgtttga g
//
LOCUS       DATLCH010000166         5466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4161584, whole genome shotgun sequence.
ACCESSION   DATLCH010000166 DATLCH010000000
VERSION     DATLCH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161584"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1019)
                     /locus_tag="VMZ02_04860"
     CDS             complement(207..1019)
                     /locus_tag="VMZ02_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HUR71294.1"
                     /translation="MSDFKHITANIDENVGIAWLTLNRPEKKNALSIALLAEISQTLR
                     DLANNDKIRCIVTSGAGDSYSSGRDLYDMRGQDNRRRTRGFGGVAEIVDIMRKLPQVT
                     VAKVRGWCLGGGLAMINGHDLVISAETAKFGMPEVIRGSYGATATPSLFHAGIPFKKA
                     FYISLTGRNLTGIEAERVGLVSDVVPEKDLDSYVETLANELASRNGATLENAKIAAYM
                     QKDLPFDMALRADDLVQHRLRYYTNPLSDVEGYLHSQKGGASVKYVKPEDRK"
     gene            complement(1158..2165)
                     /locus_tag="VMZ02_04865"
     CDS             complement(1158..2165)
                     /locus_tag="VMZ02_04865"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71295.1"
                     /translation="MEFTIIDADAHHLDGAAYKQYLPEKFRARSGPYFPSFGWDIFLN
                     DTTGRKPNNPQEYCKDLDVEKIGDAVAYPSNALSIGLVRELDLAVELAKAYNDWAYDF
                     CTESGGRVKYAAVIAPQVVAEATKEVRRAITEKGAVGVMMPTYVQQGLDLGQPQFDPI
                     YAAAQDFGVPVGFHATAQVAVGNTRFHKYIGVHMTSHPFEQMLSLVSIISNGVLDRFP
                     KLKVGFLEAGVGFLPYWMERFDEKYLIRKSEMEPLKLLPSEYIKDHRCYFTCEGEESA
                     LPLVIEQFGDECMMYASDYPHWDMEWPHSVSQVVRRKDISDTTKQKILADNAKAFYRF
                     A"
     gene            complement(2270..3964)
                     /locus_tag="VMZ02_04870"
     CDS             complement(2270..3964)
                     /locus_tag="VMZ02_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUR71296.1"
                     /translation="MFALMMRNPAQIMFLLTGVTLAYLVLPPFFFILHTSLVTDRGLQ
                     AGSFTIQHFANIIDSLGDVKTLLTNSLIYSFGSSAVALVYGTALAWLAERSDAPFRKL
                     AYISAYVSFAIPGIIKVVGWIMLLGPKAGMLNAPVIAYTGHPLFNIFSMSGMVLVESF
                     LWIPIVFLLMSTPFRSMDPSLEEAATTAGSTGWQVFQRVTFPLALPSVLAVLILTIIR
                     SLEAFEIPALIGIPAGVEVLTTKIYLQIKGGLIPKYGEASAYSIILIVLVALGLIPYY
                     RITSKTYKFTTISGKNFRPTRVALGKWRWLGGLLMLVLPLLQFFPIIAIGWSSFLPFA
                     QAPSRKALELLTFNNYVTAFNDSGVIRSVLNSLTVSISSATIAVLITFLAAWIIVRAQ
                     IRARWILDHMAMLPLVFPGIVMGIAILKMYLYLPVPVYGTIWILVLAFIARYLPYGIR
                     FSHSALLSLHKELEEGAMTSGATWFQMVRHVVIPLIMPALMAGWIYIFLITFKELSIA
                     LLLYSPGSQVVAVTIWELWDNGHVGELAAFSLIITIGTVCVGSLFRAVAQRYGLQD"
     gene            complement(4142..5338)
                     /locus_tag="VMZ02_04875"
     CDS             complement(4142..5338)
                     /locus_tag="VMZ02_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71297.1"
                     /translation="MAKLEGVKYNVGGVALDRPFKILRLGHFGFNAADLEASRRFYFD
                     LLGFFISEPAGAGYFGRHAGDHHSFALFDKKKFNERQYAGGNAKHFRLENDINQITWQ
                     VQSVTEMVEATRYFHEIGVEVMREGRAGGGGSQFHLYVWAPDDQIFELYYGIEQISWN
                     GYARPVAMRHGTAVAPTEPQLPDYQLVTDDIDKGINVLDGARYLSTMPLKYNVDGLLL
                     PQPFKITRVGPVKLFVDDYEELKHWHTQVLGFTLTEEVEWQGERCAFLRCNNEHHSLG
                     IFPKSWRKKLGLSEATSNMSFGLQLANYRQLKDAVKFLRDSGVRVETDIIPPELHPGI
                     DYAAYAFDPDGHCIELYYAMEQVGWEGKPRPKELRRKVDPNRWPDILEPLTDTYNGEP
                     YIGPWA"
BASE COUNT         1268 a         1424 c         1550 g         1224 t
ORIGIN      
        1 gattgagcgc caaatcttta atccgggaag caatgccgga gatcgaggtg tttcagtagg
       61 aaatttgctg agatcttcgt caaacacaaa ttgcctggtc tcgttgttcg gagagttcga
      121 cgggtctttt gaaaagtcac caaagatcga aatgttttga ttggctttgt gatgttgtgt
      181 cagccacgag ccctgcgcgg ctaaatctat tttctatcct ccggcttgac gtacttcacc
      241 gatgcgccac ctttttgcga gtgcagatag ccttccacat cgctgagcgg gttggtgtag
      301 tagcgcagtc ggtgctggac caggtcgtcg gcgcgcagcg ccatgtcaaa gggcagatct
      361 ttttgcatgt aggcggcgat tttggcgttc tccagggtcg cgccgttccg gctagcaagc
      421 tcgtttgcca gtgtttcgac gtaactgtcc aaatcctttt ccggcacgac gtccgagacc
      481 agtccgacac gctcagcttc gataccggtc aggtttcttc cggtcaatga gatatagaaa
      541 gctttcttga atgggatgcc ggcgtgaaac agcgatggcg tcgcggtagc gccgtagctg
      601 ccgcggataa cttcgggcat gccgaacttc gccgtttcgg cgctgatgac gagatcatgg
      661 ccgttgatca tcgccaggcc gccgcccaaa caccaaccgc gcactttggc aaccgtcacc
      721 tggggcagtt tgcgcatgat gtcgactatc tcggcgacgc cgccgaagcc gcgggtgcgc
      781 cgccggttgt cctggccgcg catgtcgtag agatcgcggc cggacgaata tgagtcgccg
      841 gctccgctgg tgacgatgca gcgaatcttg tcgttgttgg cgagatcgcg cagcgtctgc
      901 gatatctcgg cgagcagagc gatgctcagg gcgtttttct tttccgggcg attcaaggtc
      961 agccaagcga tgccgacatt ttcatcgatg ttggcggtga tgtgtttaaa atcagacatg
     1021 taaattctcc gtgggtttag tttcgttcaa atcatttctc ggattctgtt ctgtcatttc
     1081 gaggccgagc cgagaaatct ttcggaaaga tctctcactt ccttagagat gacaacatta
     1141 gtaatgatga ctgtcgacta agcaaaccgg tagaaagcct tagcgttatc ggcaagaatc
     1201 ttttgcttgg tcgtatccga aatatccttg cggcggacca cctggctgac gctgtgcggc
     1261 cactccatgt cccagtgtgg ataatccgac gcgtacatca tgcactcgtc gccgaactgt
     1321 tcgataacca gcggcagcgc cgactcttcg ccttcacagg tgaaatagca gcgatggtct
     1381 ttgatgtatt cgctgggcag caacttcaac ggttccatct cggattttct aatcaagtat
     1441 ttctcgtcga agcgttccat ccagtagggc aggaagccga cgccggcttc gagaaagccg
     1501 actttcagtt tcggaaaacg gtcgagcact ccgttggaaa taatcgacac cagcgacaac
     1561 atctgctcga agggatggga cgtcatatgc acgccgatgt atttatggaa gcgggtattg
     1621 ccgacggcaa cttgtgcggt ggcgtgaaag ccgaccggca cgccgaaatc ctgtgccgcg
     1681 gcatagatcg gatcgaactg cggctgcccc aggtcaaggc cttgctgtac gtaggtcggc
     1741 atcatgacgc cgacggcgcc tttttcggta atggcccgac gaacttcttt ggttgcttct
     1801 gcgacgactt gcggcgcaat aaccgcggcg tatttgacgc gacccccgct ctctgtgcag
     1861 aaatcgtaag cccagtcgtt gtaggcttta gccaattcca cggcgaggtc gagttcgcgc
     1921 accagaccga tggacagcgc attactcggg taagcaaccg cgtcgccaat cttttcaacg
     1981 tcgaggtctt tgcagtattc ctgcgggttg tttggttttc ttcctgtggt gtcgtttaaa
     2041 aagatgtccc aaccgaacga gggaaagtag gggccgctac gggcgcggaa tttttccggc
     2101 aagtattgct tataagccgc gccgtccagg tgatgtgcgt cggcgtcgat gatggtgaac
     2161 tccacgggtt aaaacctcca ttagctaaat atccacgttg aatcttgaac cgttgaacga
     2221 tacgagtcgc tcaacgatgc aaaacttcaa ggggaacttg gacgcaaaac taatcttgca
     2281 agccgtaacg ttgggccacg gcgcggaaca gtgagcccac acacaccgtg ccgatggtga
     2341 taatgagact gaaagcggcc aactcgccga catggccgtt atcccagagc tcccagattg
     2401 tcacggcgac cacttgcgag ccgggcgagt aaagcagcag cgcgatggag agctccttga
     2461 aagtaatcag gaagatatag atccagccag ccatcaatgc cggcatgatc aacggtatga
     2521 cgacgtggcg aaccatttgg aaccaggtcg ccccgctggt catggcgcct tcttcaagct
     2581 ctttgtgcag actgagaagc gccgaatggc tgaagcgaat gccgtagggg agataacggg
     2641 cgatgaaagc tagcaccaaa atccaaatcg tgccgtacac aggaactggc agatacaaat
     2701 acattttcaa aatcgcaatg cccatgacga tgcccggaaa gaccaacggc agcatggcca
     2761 tgtggtcgag aatccagcgc gcccggatct gcgcccgaac aatgatccag gcggctaaaa
     2821 aggttatcag taccgcgatg gtcgccgaag aaatgctgac ggtgaggctg ttcagcacgg
     2881 accgaatgac accgctgtcg ttgaatgccg tcacataatt attaaacgtg agtaactcca
     2941 gtgccttgcg tgacggcgct tgggcaaagg gtaaaaagga agaccagcct atggcaatga
     3001 tcggaaaaaa ttgcagcagc ggtagcacca gcatcaaaag accgccgagc cagcgccact
     3061 tacccagcgc gactcgggta ggacggaagt ttttgccgct gatcgtggta aacttatagg
     3121 tcttgctggt gatgcggtag taaggaatca ggcccagggc gaccagcacg ataagaatga
     3181 tcgagtaggc gctggcttcg ccgtatttgg ggatcaaccc gcctttgatt tgcaagtaga
     3241 tctttgtcgt cagcacttcg acgccggcag gaatacctat gagtgccggg atttcgaaag
     3301 cttcgagcga gcgaatgatc gtcagaatga gtactgccag cacgctgggc agggccagcg
     3361 gaaaggtgac acgctgaaag acttgccagc cggtgctgcc cgcggtggtt gccgcttcct
     3421 ccagcgatgg atccatggag cgaaatggcg tcgacatcag taggaagacg atgggaatcc
     3481 atagaaaact ttccaccagc accatgccgc tcatggaaaa aatattaaac aaaggatggc
     3541 ctgtataagc gatcaccggc gcattcagca tgcccgcttt cggcccgagc agcatgatcc
     3601 agccaacgac tttaataatc cccggtatag cgaacgagac atacgccgaa atataggcga
     3661 gcttgcggaa cggcgcgtca ctacgctcgg cgagccaggc gagtgccgta ccgtaaacca
     3721 gggcgaccgc agaactaccg aaagagtaaa tcagcgagtt ggtcagcagg gttttcacgt
     3781 cgccgagcga gtcgatgata ttggcgaaat gctgaatcgt gaaactgccg gcttgcaaac
     3841 cgcgatccgt gacgaggctc gtgtgcagga tgaaaaaaaa tggcggcaaa accagataag
     3901 ccagcgtcac gccggtgagc agaaacatga tctgagctgg attacgcatc atcaaagcga
     3961 acataaaagt acccctttca aaccggcaac gccgattccc gggccacggc caggcgcagg
     4021 agatatatat tacaaagtgc gcacataaga caattggctt aatagaggaa gaacgacacg
     4081 agtcattggt tggcaatcag gattgagact cttcgctgct cactttattt acggctagct
     4141 cttatgccca tgggccgatg taaggctcgc cgttataggt gtcggtaagc ggttcgagga
     4201 tgtccggcca gcgattcggg tcgactttgc ggcgcagctc tttcggccgg ggcttgccct
     4261 cccacccaac ctgctccatg gcgtaataaa gctcgataca gtggccgtca ggatcgaagg
     4321 cataggcggc gtagtcgatg ccggggtgaa gctccggcgg gataatatcg gtctcgacgc
     4381 gcacgccgct gtcgcgcaga aatttgacag cgtcttttag ctgccggtag ttcgctaatt
     4441 gcaagccgaa agacatattt gaagtggcct cgctcaagcc gagctttttg cgccatgact
     4501 tgggaaatat ccccagcgag tggtgctcgt tattgcaacg cagaaaggcg catcgttcgc
     4561 cctgccactc aacttcttcg gttagagtga agccgagcac ttgggtgtgc caatgtttaa
     4621 gttcttcgta atcgtcgaca aatagcttca ccggaccgac gcgggtgatt ttaaacggct
     4681 gcggcagtag taagccgtcg acattatatt tgagtggcat cgtgctgaga taacgggcgc
     4741 catcgaggac attgatgcct ttgtcgatgt cgtcggtgac gagctggtag tccggcagct
     4801 ggggttcggt gggcgccacc gccgtcccat ggcgcatggc gaccggacga gcgtagccgt
     4861 tccagctgat ctgctcgatg ccataataaa gttcgaagat ttggtcgtcc ggcgcccaga
     4921 cataaagatg aaactgcgag ccgccgccgc cggcacgtcc ctcgcgcatg acctcgacac
     4981 cgatttcgtg aaagtatcgg gtcgcctcaa ccatttcagt cacgctctgt acttgccagg
     5041 ttatttggtt gatgtcgttt tccaaccgga aatgtttagc gttgccgcca gcgtattgac
     5101 gctcgttaaa tttctttttg tcgaatagcg cgaatgaatg gtgatcgccg gcgtggcgcc
     5161 cgaagtagcc tgcgccggca ggttcgctaa taaaaaatcc caataggtcg aaatagaatc
     5221 gccggctggc ttccaggtcc gcggcattga aaccgaagtg gcctaggcga aggatcttaa
     5281 acgggcgatc gagggctacg ccgccgacgt tgtattttac tccttcgagc tttgccataa
     5341 tttctcctca tttacggcga gcgatgcctc attggaattg atggctggct attttcccgg
     5401 tgcggtgatc acagcttccg gcgtatatgc cgatgaaccc gatgaattgg tcgctttgac
     5461 acgata
//
LOCUS       DATLCH010000167         5681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1948225, whole genome shotgun sequence.
ACCESSION   DATLCH010000167 DATLCH010000000
VERSION     DATLCH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948225"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..690
                     /locus_tag="VMZ02_04880"
     CDS             172..690
                     /locus_tag="VMZ02_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71298.1"
                     /translation="MSAALAFAILLSAVSLAAAAERSWPGAPPDCWKEERIIHNTEFA
                     DLWKANTVFKKLPVGEIKPGVYSPNKGYYFIVENGRPHGAVIIFAEQPYLIRIEFSEL
                     FGLADVKWINEKLLFIRPWWGRIAATDLIYDVEAERVIHSDGVVDAYQAFLQYRESCP
                     MNGCECIKKKLE"
     gene            complement(754..1587)
                     /locus_tag="VMZ02_04885"
     CDS             complement(754..1587)
                     /locus_tag="VMZ02_04885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR71299.1"
                     /translation="MAETVLRDEHEMLRPGLGRLKGKVAIVTGANSGIGRATSRLFAR
                     EGAKVVCCDIQETISPRIDSLIKEKEAGEALFAQCDVTRQEDLDRAVKTAVDSFGKVD
                     ILYNNAGAGIRKKIHEHSDEEWNFVLNTNLNAMYRGARAVMPQFIKQGSGNMVTTAST
                     FGLLASAEYPGYCATKAAIINLTREMAIDYGPLGIRINSVCPGAIETPRFRGFPPRPT
                     LGEGMTDEQRKRMGASNKALLRMGRPEEIAYGVLFLVSDEASFVTGHALVVDGGQTIA
                     V"
     gene            1743..2132
                     /locus_tag="VMZ02_04890"
     CDS             1743..2132
                     /locus_tag="VMZ02_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HUR71300.1"
                     /translation="MAAIRSFRDLEVYIQARQQAKNVFLVSKRFPRDERYSMTDQIRR
                     SSRAVNAMIAEAWARRMYRASFVNKIDEALGEAMETQAWLDHAFDCGYLNGEEHKGLD
                     DIWQRIGAMLNRMIQRADDFCRTADRL"
     gene            complement(2184..3176)
                     /locus_tag="VMZ02_04895"
     CDS             complement(2184..3176)
                     /locus_tag="VMZ02_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71301.1"
                     /translation="MSLKTILIAIALVMAALIETAQVSAQERLRLAWAGFSPTNSPIW
                     VMEDRKLLQKQGVSPEIIAINASPTVLQALLADEIDVASVSVTTLTSSRLQGADTVLI
                     IGVVPTFVDHIISVNSITKVEQLKGKIGGVNRLGSTSDLGLRLALRKLGVDPEKDVKI
                     LPVGGNPERFASLSKGLIQFTIMPEPFLTQAEKLGFRNLYNVTDLKIPFWWNGILSRE
                     AIVKTKRPLMLKLTRAMIEAVHIIKTEKEYAKSLFKKNLGVSDPEGLERAYKDYSAVF
                     PEVPYPNADGVKTMLDDLAPRNPKAGAADPKTYVDMSLVAELEKSGFIKQLYRR"
     gene            3357..4328
                     /locus_tag="VMZ02_04900"
     CDS             3357..4328
                     /locus_tag="VMZ02_04900"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71302.1"
                     /translation="MAKKGILITGGSGLVGAYAVQMLMGRGERPVIFDVALNERLLGA
                     VGVDTSKVTMIRGDMADLPALITAIRDNDCDQVIHLAAFLGEEVQRRPYSGVRLNFMG
                     TINVFEAARLENVKRVVFPSSGTVYLGSLGEGVKQIDENIPMNPPSVYAATKASCEFM
                     GRAYAKRYGFEFVCLRYTGGLYGPSPAALKATREIAIQQMVRAAVKGEAAKINWPYGP
                     AEILYGKDAAKGTVLAVCKDKFKDSLFHIGNADLVSGEDIVQAIAKNFPGSKIEIVKT
                     ANPMPYPESRLSSDFSRAKEQLGYEPEYPIDRAIEDYGATLRKLENL"
     gene            4438..5520
                     /locus_tag="VMZ02_04905"
     CDS             4438..5520
                     /locus_tag="VMZ02_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71303.1"
                     /translation="MSLGKWILPIVLGFALFLQPLWAAQPVAPNAGFDEKAVGDFYRG
                     KTVRIVVGFSAGGGYDQYSRLIARHLSRHIPGNPNVLVDNMAGAGSMVAANHIYNAAP
                     KDGTVIGNISGPIVLEQLFGNPAVQFDMGKFRYLAVPVSESYLMIAHKRTGITKFDDV
                     RGEKGKLLTVGAIPGSTVEHAPIMVKEVLNTNLKVVSGYKGTADVRLALDSGEVDGFF
                     NTWTSSKVTSMDKVKSGEWVILAQLTETPMKDLVVPNVPTIQKISRSEEQRLLLKYGT
                     STPNDFGKVYILPPGTAADRSAALESAFVKAFKDKELIAEADKGRLEIDPLIGNTITK
                     LVTEFLGMPNDLKNKLQTVLKTPVKK"
BASE COUNT         1273 a         1494 c         1599 g         1315 t
ORIGIN      
        1 ttgttcttcc attttattta ctaccttcac tactgagatg cagctacatg cgccagctcg
       61 aaactcgcgg aagaaaagag ttgggcaacc ggaggcaatt ttccgccctg ggattagcgg
      121 gctatggtat ttattatcac aaacaaattt cttgtcgtcg gggaggtaat catgtcagct
      181 gcgctagctt tcgccatttt attgagtgcc gtttccttgg ccgcagcagc ggagcggtcc
      241 tggcccggcg cgccgcccga ttgttggaaa gaagagcgga ttatacataa caccgagttt
      301 gccgacctgt ggaaagccaa tactgtgttc aagaaattgc cggtcggaga aataaagccc
      361 ggagtttatt cccccaataa aggctactac ttcatcgttg agaatggccg gcctcacggc
      421 gcggttatta tctttgcaga acagccttat ctgattcgca tcgagttctc ggagctgttc
      481 ggtctggccg acgtgaagtg gatcaatgaa aagctgctat tcatccgacc ctggtggggg
      541 cggatcgcag cgacggatct aatttacgac gtcgaggccg agagggtgat tcacagcgac
      601 ggcgtagtcg acgcctatca agcctttctc caataccgcg agagctgccc gatgaacggc
      661 tgcgagtgta tcaaaaagaa gttggagtga tggagtattc gcttttgtac gccgcaacac
      721 tccatcactc gcatgttcta ttctcccatt tcgtcatacc gcgatggttt ggccgccgtc
      781 gaccaccagc gcgtggccgg tgacgaacga ggcttcgtcg gatacgagaa agagcacgcc
      841 gtaggcgatc tcttccggac gacccattcg cagcagcgct ttattgctgg cgcccatcct
      901 ctttctctgc tcgtccgtca ttccttcgcc cagtgtcggc cggggcggga aaccgcggaa
      961 gcggggagtt tcaatggccc cagggcaaac cgaattgatg cgaattccca gcgggccgta
     1021 atcgatggcc atttcgcggg ttaaattgat gatcgctgcc ttggtggcgc agtagccggg
     1081 atattccgcg gaggcgagca gtccgaacgt cgacgcggtc gtcaccatat taccgctgcc
     1141 ctgtttgatg aactgcggca ttaccgcgcg ggcaccgcgg tacatggcgt tcaggttggt
     1201 attcaataca aaattccatt cttcgtcgct gtgttcatgg atttttttgc gaatcccggc
     1261 gccggcgttg ttgtagagaa tatctacctt gccaaagctg tcgaccgcgg tcttgacggc
     1321 gcggtccaaa tcttcctgcc gggtgacgtc acactgggcg aataatgctt ctccagcctc
     1381 tttttctttg attagactgt cgatgcgcgg tgagatcgtt tcctggatat cgcagcacac
     1441 tactttcgcg ccctcgcgcg cgaacagccg cgaggtggcc cggccgatac cgctgttggc
     1501 accagtgacg atcgcaacct tgcctttgag tcgtccgagt cctggacgca gcatctcgtg
     1561 ttcgtctctc aaaacggttt ccgccataaa tttgcctcct gagtaaagtg tgaagttgtt
     1621 cgcttcactt atcccagggc ggcgaggaaa ttcaaccgaa gcggagggat tgagaataga
     1681 ggatagacga ttgaggatgg cgtctcggag tagtatacgg acgattgcga tccggagggg
     1741 atatggcagc tattcgaagt ttccgtgact tggaagtcta tatacaagca cgccagcagg
     1801 ctaagaacgt ctttctggta tcaaaacgat ttccaagaga cgaaagatac tccatgaccg
     1861 accagatacg gcgttcatcg cgggccgtga atgcgatgat cgctgaagca tgggcgcggc
     1921 ggatgtatcg ggcgtcgttt gttaacaaaa tcgacgaagc gttaggcgag gcgatggaaa
     1981 ctcaagcatg gctcgatcat gcctttgact gtggttatct caatggagag gaacacaaag
     2041 ggctggatga catctggcaa cgcattggcg caatgctgaa ccgaatgatc cagcgtgccg
     2101 atgatttctg ccgaactgcc gatcgattat aacattccgg cgccatcctc tatcgtcaat
     2161 cctccattcc cgacgcgcac ttcttagcgg cgatacagct gctttataaa gcccgacttt
     2221 tccaattccg cgaccaaact catatcgacg taggtcttcg gatctgctgc acccgctttg
     2281 ggattgcgcg gcgccagatc gtcgagcatg gttttgacgc catcggcatt gggataaggg
     2341 acctccggga aaacggcaga gtaatcttta taggcgcgct cgagaccttc gggatcgctc
     2401 acgccgagat ttttcttgaa tagcgacttg gcgtattcct tctcggtttt gatgatatga
     2461 acggcttcga tcatcgcgcg ggtgagctta agcatcagcg gccttttggt ttttacgatc
     2521 gcttcgcggc tcagaatgcc gttccaccaa aacgggatct ttaaatcggt gacgttataa
     2581 aggttacgaa agccgagctt ttctgcctgg gtcagaaatg gctcgggcat gatggtgaac
     2641 tggatcaagc ctttggatag tgacgcgaaa cgttccggat tgcctccgac cgggagtatc
     2701 tttacatcct tttccggatc gacaccgagt ttgcgcaagg ccaggcgtaa acctaaatcg
     2761 gaggtgctgc caagacgatt aacgccgccg attttccctt tcagttgctc gaccttggta
     2821 atgctattaa ccgaaataat gtggtcgaca aacgtcggca cgacgccgat gatcaagacc
     2881 gtatcggcgc cttggaggcg tgaactggtg agggtggtca ccgagacgct ggcgacatcg
     2941 atttcatcgg cgagcaaagc ttggagtacg gtggggctgg cgttgatcgc gatgatttcc
     3001 ggcgagacgc cctgtttctg cagcagttta cgatcttcca tgacccagat tgggctgttg
     3061 gttgggctga agccggccca agccagtcgt agtctttcct gtgccgagac ctgcgcggtc
     3121 tcgattagcg cagccatgac tagggcaatt gcgattaata tggtcttaag ggacatgggg
     3181 ctagcctcca ctcgcttagt ttagacgtcg cagcgggaat atcgcggtaa tccaatcttg
     3241 ggtcaatact cgagccggag gctgccatat cgccatcaga catgccaaga atatgcgttg
     3301 actcaatatc tcgtttgata tactgagtgc acgactttgc tcggggggaa caatacatgg
     3361 ccaagaaggg cattctgatt accggcggat caggtttggt cggcgcctat gcagtgcaga
     3421 tgctgatggg acgcggcgag cgcccggtca tcttcgatgt cgcgctcaac gaacgactgc
     3481 tcggcgcagt cggtgtcgac accagcaaag tgacgatgat ccgcggcgac atggcggatt
     3541 tgccggcact gataaccgct atccgcgaca acgactgcga ccaggtaatt catcttgccg
     3601 ccttcctcgg tgaggaagtc cagcgacgcc cgtatagcgg cgtgcgccta aacttcatgg
     3661 ggacgatcaa tgtcttcgaa gcggcgcgtc tggaaaacgt caagcgagtt gtttttccca
     3721 gttcgggaac cgtctatctc ggctcgcttg gggagggtgt caagcagatc gacgagaata
     3781 tcccgatgaa tccgccgtcg gtctatgccg cgaccaaggc gagctgtgaa ttcatggggc
     3841 gcgcttatgc gaaacgctat ggtttcgagt tcgtatgcct gcgctacacc ggcggcctgt
     3901 atgggccgtc cccggcggcg ctcaaggcaa cgcgggagat cgcgattcaa caaatggtgc
     3961 gtgccgcggt caaaggcgag gcggcaaaga tcaactggcc ctacggtccg gcggaaattc
     4021 tctacggcaa ggatgccgcc aagggcactg tgctggcggt ttgtaaagac aagtttaaag
     4081 actcgctttt ccatatcggc aatgccgatc tggtcagcgg cgaagacatc gtccaagcga
     4141 tcgccaaaaa ttttcccggc tcgaagatcg agatcgtcaa aaccgccaat ccaatgcctt
     4201 atccagaatc tcgcttatcg agcgatttct cgcgcgccaa ggagcaactc ggctacgagc
     4261 ccgaataccc gatcgaccgg gcgatcgagg attatggcgc gacgttgagg aaactggaaa
     4321 acctctagtc cggcaatctg ctgatctgaa gttgtcgtct gcgttatcgt ctaaacaatc
     4381 gacacgccat gcttccacgg cgtgaaacta gttagccata tgggggaaca gcaataaatg
     4441 agtttaggaa agtggatttt gccaattgtc ttgggtttcg cgctttttct gcaaccgtta
     4501 tgggcggccc agccggttgc gccaaacgcc ggctttgatg agaaagcggt cggcgatttc
     4561 tatcgtggta agaccgtgcg catcgtcgtc ggtttttccg ccggcggtgg atacgaccag
     4621 tattcccggc taatcgcgcg ccacctgtcg cgccacatac ccggcaatcc caatgtgctc
     4681 gtcgacaaca tggccggcgc cggcagcatg gtcgcggcca atcacattta taatgccgcg
     4741 cccaaagacg gcacggtcat cggcaacatt tccggtccga tcgttttgga gcagctcttc
     4801 ggcaatccgg cggtgcagtt cgacatgggc aagttccgct atctcgcggt accggtaagt
     4861 gagagctatc tgatgattgc gcacaagcgc accgggatta ccaagtttga cgacgtccgc
     4921 ggggaaaaag gcaagctgct gaccgtggga gcgattcctg gctcgaccgt ggagcatgcg
     4981 ccgatcatgg tcaaagaagt cctcaacacc aatcttaaag ttgtttccgg ctacaaaggt
     5041 accgccgatg tgcgcttggc cttggatagt ggcgaggttg acggcttctt caatacctgg
     5101 acctcttcca aagtgacatc catggacaag gtgaaaagcg gcgagtgggt gatcttggct
     5161 cagcttaccg aaaccccaat gaaagatctg gtcgttccga acgtgccgac gattcaaaag
     5221 atctctcgct cggaggagca gcgtcttttg ctcaagtatg gcaccagcac gccaaacgat
     5281 ttcggcaaag tatatattct gcctcccggc acagcggctg atcgcagtgc ggcgcttgaa
     5341 agcgctttcg tcaaagcatt caaagataag gaactgatcg cggaagccga caaaggccgg
     5401 ctggaaatcg atccgttgat cggcaatacc atcaccaagc tggtcaccga gtttctcggc
     5461 atgcccaatg atttgaaaaa caagctacag accgtgttga agacgccggt gaaaaaataa
     5521 ttattgtcga tctcagtaac cgcaagcgag gatcgctatg tcacgaatcg ttagtcaatt
     5581 tgccctgatc gttgcggcgc tcttgacgct cagcccggcc gacgggctgg cgcagactga
     5641 aaccctcgac gaactttaca agaaggctgt taaagaagac g
//
LOCUS       DATLCH010000168        10349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9921843, whole genome shotgun sequence.
ACCESSION   DATLCH010000168 DATLCH010000000
VERSION     DATLCH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9921843"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VMZ02_04910"
     CDS             complement(<1..553)
                     /locus_tag="VMZ02_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HUR71304.1"
                     /translation="MQTPKSGLFATAFSALLSPVLRYIDKPSQPRYHGEIQLKGLQQT
                     VDVSFDGYAVPRVSAANEQDLFFAQGYLHAQERLWQMELSRRFLGGRMAELFGDFALP
                     WRELSGQFRGHKTSDLDFFIRLLGIRQSALESLRILTADDSRRLNAYSEGINRYIERC
                     GRKLPWEFRVLRFVPEPWTPTDSL"
     gene            complement(586..1185)
                     /locus_tag="VMZ02_04915"
     CDS             complement(586..1185)
                     /locus_tag="VMZ02_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR71305.1"
                     /translation="MIKLYDFKSSPNCQRVKVVLAEKNLPYEIVPVDLTKKEQKAPDY
                     LKMNPYGKVPVLVDDSTVLYESLIINEYLEEKYPSPALMPKDPSKKAKARILTDYGMA
                     HFDSAYQKLRMESMKDAKEQSQPIIEGAKAELKKLLQRFEDELGDQQYLLGDFSLVDA
                     NLIPRFSRLEGFGVLPDPALPRLAKYIERMKARPSVKAL"
     gene            1339..1860
                     /locus_tag="VMZ02_04920"
     CDS             1339..1860
                     /locus_tag="VMZ02_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR71306.1"
                     /translation="MTAAESAKILVGGLKGAGISLIATLPDINLSELLREVEEAQDVI
                     HVPLCREEEGMGICAGGYLVGKKCAAIMQNGGFFNSNNAIVSTLLQYQIPLLLLIYYA
                     GDIGDRTFATTGSMTEPVLQALGIRYYVMRDIADAPELIKRAQVLAEDSKKPIALLLT
                     KEVLGRRPALQSL"
     gene            1868..2473
                     /locus_tag="VMZ02_04925"
     CDS             1868..2473
                     /locus_tag="VMZ02_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUR71307.1"
                     /translation="MKRYDCLQALALQVKDALVVSSAGAMTLEWNSLHPSDGNFRVRT
                     LGLCSSIALGMALGLPHRKVVALDGDGSLLMNLCSLPTIARMKPGNLVHIVFDNEVYE
                     ASGSKKTATATGADLVAIACAAGIGNSQWAKTVDEFAAVTAAALSGNDLCFIGAKVTS
                     ERTAVPPFPIDEVENKYRFIRHIENTEKIEIFKTNLPASYT"
     gene            2487..3503
                     /locus_tag="VMZ02_04930"
     CDS             2487..3503
                     /locus_tag="VMZ02_04930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71308.1"
                     /translation="MKIDIEFNSGAQLPMDAIPELARQAEAHGFDCAWAGEANNKDPT
                     VMLSAIAAVTRQLKIGSAIYHILGRTPATLALQATGLDELTGGRFLLGIGSSNPTIAK
                     WHGQIFDHPLGRVREYIDIVRAAMRGDKLAFNGKYFSAENFKMAFKPSGRTIPIYLAA
                     FGSQMCRLAGQITDGVLINMANPTEIRRIAEEVRAGARQVGKDPANMEIICKIRCSIA
                     PRYDTARDALSHALTYYALADYYRDLLGRMGFAGEVEAMRAAWKSGGFHAARKLISDP
                     MFGSLPLIAATSAPQVVEQIKPYVEAGATRVILPYVAASADVVGEMKSFIEYWSPAVT
                     SSLG"
     gene            3516..3917
                     /locus_tag="VMZ02_04935"
     CDS             3516..3917
                     /locus_tag="VMZ02_04935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUR71309.1"
                     /translation="MSFDLFNPAGLEAPVGYAHAAKITGGTTVHIAGQAPFDVNGQVV
                     GKGNFVAQFTQVMSNLKTAIEAVGGSPNQYAVLTIYVTELESYWNNKKPLGGAYRAVF
                     GKHFPAITLVEVKRLYNPDCMLEISGIAVID"
     gene            4107..4316
                     /locus_tag="VMZ02_04940"
     CDS             4107..4316
                     /locus_tag="VMZ02_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71310.1"
                     /translation="MLRTPEISLLNREHDADLFETVTEEEIIFLWRSIKGRGLSEDEA
                     WQAEVTGMALDMAFAKSKCCALTEH"
     gene            4465..4659
                     /gene="rpoZ"
                     /locus_tag="VMZ02_04945"
     CDS             4465..4659
                     /gene="rpoZ"
                     /locus_tag="VMZ02_04945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HUR71311.1"
                     /translation="MSRITVEDCLEKVTNRFELVLLASKRARHLSQGAKPLIDSDNRE
                     VVVALREIAAGKIRKIPTDS"
     gene            4764..5726
                     /locus_tag="VMZ02_04950"
     CDS             4764..5726
                     /locus_tag="VMZ02_04950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF005143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HUR71312.1"
                     /translation="MPPMAEEETSQTNIPFVEFDLPKIVDEEEPDSSRKALVPFDPLQ
                     RYLAEIRRFAVLSREEEHKLAVEYKEFGNLEAAYKLVTANLRLVVMIAREYQKSFKTL
                     LDLVQEGNMGLMEAVKNFDPYRGVRFPSYAVWWIRAYMIRYIMNDWRMVKIGTTQAQR
                     KLFFNLQKEKERIEAEGLTVGPKLLAQRLNVKEDEVIEMEQRLASRDLSVDVPIGDDD
                     EATLLHFLPDHKQNPEEEYAESQYQQMLREKMEQFAKTLKDKELVIYRERLLNEDPST
                     LREIGDKYGISRERVRQIEERVKKKLKTYLSKELKDATDITGAD"
     gene            5846..6298
                     /locus_tag="VMZ02_04955"
     CDS             5846..6298
                     /locus_tag="VMZ02_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUR71313.1"
                     /translation="MQFEKQVEIHVPREKVWKFIWDIDRFIACVPGCKDAKTVEEGKR
                     YTATMVEKVGPFKVEFPTTIEVLERAELSHIKAQASGADNKIGSRMKIDLDVNLREQD
                     NTTVVSFVAGVDILGKLAALGHGIIKRKADQVLDEFAQAVKTTLEGDN"
     gene            6300..7151
                     /locus_tag="VMZ02_04960"
     CDS             6300..7151
                     /locus_tag="VMZ02_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HUR71314.1"
                     /translation="MLKRFRLEEPESVADVSELLGRFGDSAKIYAGGTELLLAMKEGL
                     VHYERLINVKNIKGLGEVAGDNGTIRIGALCTHHELENSPLLQQKLPSFVKLEQNVAN
                     VRVRQAGSIGGNICFAEPHADPGTLFIALDAVMVAEKSSSKREISASDFFVDAFETSL
                     ATDELLTEIRIPTPGANRRSAYLKFGYLERPSVGVAVALTFDSAGVNDVKIAVGCAGP
                     APRRVTEAEALLNGKSKDEAVSLLAQAGAVAGRASEAISDLHGAQDYKEHIVGVLLKR
                     AFNSIVS"
     gene            7186..7692
                     /locus_tag="VMZ02_04965"
     CDS             7186..7692
                     /locus_tag="VMZ02_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUR71315.1"
                     /translation="MSKQMTRHADTQSISVSFTLNGRSQEIEVEPHELLLDVVRDRLG
                     LTGAKRSCDVQVCGACTLLVDGRPVSACTTLAFEVRGRSVMTIEGLAEDGKLHPLQNA
                     FIEHGGFQCGFCTPGMLLASKALLDENPDPSEAELKHFMHGNLCRCTGYKKIIESIMA
                     AAKEMRNR"
     gene            complement(7789..9210)
                     /locus_tag="VMZ02_04970"
     CDS             complement(7789..9210)
                     /locus_tag="VMZ02_04970"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR71316.1"
                     /translation="MAYKDLRDYVAALEKHGKLKRVTKKVDKDWEIAAVCRQLFKKIP
                     PAQRPALVFENVAGFDIPVVAGVLGASREIYALGLQTETVEGINRKWDQALEHPIEPR
                     LVKHAPCKENILHGKEVDITKLPVPTWTVGEDPGPFFTSPYLITKDPETGVRNVGTYR
                     MQVKGPNKTGLLIGKRQDMAWHMYKNNAQNKPTPLAVVIGADPSIGYVSVSKISEALD
                     EFAVAGALRGEAVDMIACETVPLEVPASAEIVLEGEIPPNAFEHEGPFGEYTGYMGPA
                     GNEPFFNIKCMTFRNKPLYQAFISQMPPSESSCIRGVGREWPLFKHLKDILRLPIKDL
                     RLKEAGGSGAYLVISLKKQFDGQVRQLMYGAWSQRTGFGKITVVVDEDIDVWDDFAVD
                     WALSWHVRPEKDVYIERDVQAVGLDPSQAPADVVQHHASRYVGSRVAIDATRKHKYPA
                     ISLPPKEHLDLVTSRWKEYGIED"
     gene            complement(9337..10260)
                     /locus_tag="VMZ02_04975"
     CDS             complement(9337..10260)
                     /locus_tag="VMZ02_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUR71317.1"
                     /translation="MSVVFWKELADHFSSRRFMILLILIVFSGLWAAYATGQAIRANT
                     DTVPTQYVFLLLLTSSGDTLFSLATFLGFFGPLVGITLGFDSISGEYARGTLSRVLSQ
                     PIYRDSLINGKFFAGLTTVGVLWGSILLLVIGLGITLLGFPPNAEELWRMIIFTVVGI
                     FYVGFWLALAMLFSLLFQKTVTAALASIAVWLFLSLFVAVISGAIANMVVPDATTPEA
                     VARKSDIENIVGRVSPSTLFNESVGILLNPAARVFGMALQSQAEGILPTPLALGQSLN
                     LIWPHITTMFALVAVCFGISYIIFMRAEIRA"
BASE COUNT         2502 a         2653 c         2826 g         2368 t
ORIGIN      
        1 tcaggctatc cgttggcgtc caaggttcgg ggacaaatcg caacacgcga aactcccatg
       61 gaagctttct accgcatcgc tctatgtagc gattgatacc ctcgctatat gcattcagac
      121 gccggctgtc gtcagccgtt aagattcgta gcgattctag cgccgattgg cgaataccca
      181 atagtcggat aaaaaaatcc agatcgctgg tcttgtggcc acggaattgg cccgagagct
      241 ctcgccacgg cagcgcgaag tcaccgaata attcggccat gcggccgccg agaaagcgcc
      301 ggcttagctc catttgccag agccgctcct gcgcgtgcag ataaccttgg gcgaaaaata
      361 agtcttgttc atttgcggcg ctcacacggg gcaccgcgta accatcgaaa gacacgtcga
      421 ctgtttgttg taaacccttg agctggatct cgccgtgata gcgaggctgg gatggcttgt
      481 cgatataacg aagaaccgga gaaagcagcg ccgaaaaggc ggtcgcgaac aaaccgcttt
      541 tcggagtctg catgggtaat tgaactgcgg gaattctcag agctactata aagccttgac
      601 cgatggacgg gctttcatcc gttcgatgta tttcgccagg cgtggtaaag ccggatcggg
      661 caggactccg aagccttcca atcggctgaa ccgcggtatc aagttggcat ccaccagaga
      721 aaaatcgccg agcaaatact gctggtcgcc aagctcatct tcaaagcgtt gcaggagttt
      781 tttaagttcc gccttggcgc cctcgatgat cggctggctc tgctctttgg cgtctttcat
      841 cgactccata cgaagtttct gataagcgct atcgaaatgg gccatgccgt aatcggtaag
      901 gatgcgcgcc ttggcttttt ttgacggatc tttgggcatc agcgccgggc tcgggtattt
      961 ttcctccaaa tactcattga tgatcaacga ctcgtaaagc acagtgctgt cgtcgaccaa
     1021 cactggaact ttgccgtagg gattcatttt taaatagtcc ggagccttct gttctttttt
     1081 cgtcaggtca actgggacaa tttcataagg tagatttttt tcggctaaaa caactttgac
     1141 ccgttggcaa ttgggcgaag atttaaaatc gtaaagcttg atcatgacgg cggtcctttc
     1201 gagcaaaaag atatgctgga tctatactag gaaagagact aaaggaaaag tcccgaccga
     1261 gcagcgtcgt tgcttgaagt acccttttat tgatgttaag cggagaacgg tccggcagga
     1321 ttttaggaag ttttcataat gactgcagcg gaatcggcga aaatacttgt aggtggattg
     1381 aaaggagcgg gcatcagtct gatcgctacc ttgcctgata tcaacctttc cgaactgctg
     1441 cgcgaggtag aagaagcaca ggatgtaatt catgtgccac tttgtcgcga agaggagggc
     1501 atgggtatct gcgccggcgg ctatttggtc ggtaaaaaat gcgccgcgat catgcagaac
     1561 ggcggcttct tcaacagcaa caacgctatc gtcagtacct tgctgcagta tcagatcccg
     1621 ctgcttttgc ttatctatta cgccggcgac atcggcgacc gaacgtttgc tacgaccgga
     1681 agtatgaccg agccggtgct gcaagctctg ggaatccgct actacgtcat gcgcgacatc
     1741 gccgatgcgc cggagttgat caaacgcgcc caggttctcg ccgaggactc gaaaaaaccg
     1801 attgcgcttt tgttgaccaa ggaagtattg ggccgacggc cggcgctcca atcgctctga
     1861 ggcaacaatg aaacgttacg actgtttaca ggcactggcg ctgcaagtga aagatgccct
     1921 tgtggtgagt tccgcgggtg cgatgacttt ggagtggaac tcactccatc cgagcgacgg
     1981 caattttcgt gtccgcacct taggattgtg ctcgtcaatc gctctcggaa tggcacttgg
     2041 actaccgcat cgcaaagttg tcgcactcga cggcgatggt tcgttgttga tgaatctttg
     2101 ctcgctcccg acgattgccc ggatgaagcc gggaaacctc gttcacatag tattcgacaa
     2161 cgaagtttac gaagcgtccg gcagcaagaa aaccgctacg gcgaccggtg ccgatctcgt
     2221 cgctatcgcg tgcgccgccg gcatcgggaa ttcccagtgg gcgaaaactg ttgacgagtt
     2281 cgcggcagtt acggcggcgg cgttgagcgg caacgatctc tgtttcatcg gtgccaaagt
     2341 cactagcgaa agaaccgcgg tgcctccgtt tcctatcgac gaagtcgaga ataagtatcg
     2401 cttcattcgt cacatcgaaa ataccgagaa gatcgagatc ttcaagacta atttgccggc
     2461 gagttacacg taagctcgac gccttcatga aaatcgatat cgaatttaat tcgggagccc
     2521 agctaccgat ggacgccatc ccggagctag cacgtcaagc cgaagcgcat ggctttgatt
     2581 gcgcttgggc tggggaggca aataataaag atccgactgt gatgctgtcc gcgatcgcgg
     2641 cggtgacccg tcagttgaag atcggttcgg cgatctatca tattctcggt cgcacgccgg
     2701 cgacgttggc gttacaggcg acgggactcg acgaattgac tggcgggagg tttcttctgg
     2761 gcattggctc gtccaatccg acaatcgcca aatggcatgg ccagatattc gatcatcccc
     2821 tcggccgcgt gcgtgagtat atcgatattg tccgggcagc catgcgcggg gataagcttg
     2881 ccttcaacgg taaatatttc tccgccgaga attttaagat ggcgttcaaa ccttcgggcc
     2941 gaacgatccc catctatttg gcggcgttcg gttcgcaaat gtgccgcctt gccgggcaaa
     3001 tcaccgacgg cgtgctgatc aacatggcca atccaacgga gattcgccgc attgccgagg
     3061 aggtgagggc gggggcgcgg caggtcggca aggatccggc gaatatggaa ataatctgca
     3121 agattcgctg ttccattgct ccccggtatg atactgcgcg ggacgcgcta agccatgctc
     3181 taacctatta tgcgctcgcc gattattatc gtgacctgct cggcagaatg ggatttgccg
     3241 gagaggttga agcgatgcgc gcagcttgga aatccggcgg atttcacgct gcgcgcaagc
     3301 taatctcgga tccaatgttc ggtagtttgc cgctgatcgc cgcgacctcg gcgccacagg
     3361 tggtcgagca gatcaaacca tatgttgaag ccggggctac ccgcgtgatt ttaccctacg
     3421 tcgcggcatc agccgacgtt gtcggcgaaa tgaaaagttt tatcgagtac tggagcccgg
     3481 ccgttaccag ttcgctcggc taagaggagt atctcatgtc gttcgattta tttaatcccg
     3541 cgggattgga agcgcccgta ggatatgctc atgccgccaa gatcaccggt ggtacgacgg
     3601 ttcacatcgc cggtcaagcg ccgttcgacg ttaacggcca agtcgttggc aagggaaatt
     3661 ttgttgctca gttcacccaa gtgatgagta atctcaagac agcgatagaa gcagtcgggg
     3721 gaagccccaa ccaatatgcc gtactgacaa tctacgtaac ggagttggaa tcgtactgga
     3781 acaacaaaaa accgctcggc ggcgcctatc gcgcggtgtt cgggaaacat tttcctgcca
     3841 ttacattggt cgaggtgaag cgcttgtata atccagattg catgttggag atttctggaa
     3901 ttgcagtgat cgattgaatc gtaagagcca aggagttttc ctgcttgttt ggcgtatttt
     3961 gctcctgatc agacaaattt gctcgttctc gggtacctat acgtgccagc tcgatggtga
     4021 accaacgcag tttcagcacg ttagtcattg gcctgtgaat tgctcaacac aaagccatta
     4081 tcttcaaagg tggcttcgag actcacatgt tgcgtacccc cgaaatttct ttgctaaacc
     4141 gcgagcacga tgcagatctt ttcgaaaccg tcacagagga agaaatcatt tttctttggc
     4201 gctcgatcaa ggggcgcggg ctttccgagg atgaagcttg gcaagccgag gtgacaggaa
     4261 tggcactcga tatggcgttt gccaagagca agtgttgcgc gctcaccgag cattagataa
     4321 ccctgccggc cgttagattt gcgctcgacc cgtcagattt ccgcacgcct gggcatagta
     4381 cttcccgctg ccttaactgc tttactttat tgatcttagc gagaaaatct gcttcaatct
     4441 tcctaggata aaggggagat tgacatgtct cgcatcaccg tcgaagactg tttagaaaag
     4501 gtcaccaatc ggtttgaatt agtactcttg gcgtcgaagc gcgcacgtca tttgtctcag
     4561 ggcgccaaac cgctcattga ttccgacaat cgcgaggtcg tcgtcgccct gcgcgagatc
     4621 gccgccggta aaatccgaaa aatcccaaca gactcctaaa acttcatcgc ggcgtcaatt
     4681 ctcgccctga gatccacctg catagactta aattgacacc ttcgcgggcg ccacttcatt
     4741 ggggcatttt gtgaaggtgt gtgatgccgc caatggccga agaagaaact tcccaaacta
     4801 atattccctt cgtcgaattc gatctgccga aaatcgtcga cgaggaagag ccggacagca
     4861 gccggaaggc gctcgttccg ttcgatcccc tccaacgata tctcgcagag attcgccgtt
     4921 tcgccgtcct tagccgcgag gaagagcata agcttgccgt tgaatacaaa gaatttggca
     4981 atctagaagc agcttataaa ttagtgactg ccaatctccg tttggtggtg atgatcgccc
     5041 gtgaatacca aaaatccttc aagactttgc tcgacctggt acaggaaggg aacatgggac
     5101 tcatggaagc ggtgaagaat ttcgatcctt atcgcggtgt acgcttcccg tcctatgccg
     5161 tatggtggat tcgcgcctac atgatccgtt acatcatgaa cgattggcgc atggtcaaaa
     5221 tcggcaccac acaggcgcag cgcaaattgt ttttcaatct gcaaaaagaa aaagagcgca
     5281 tcgaggccga ggggcttacc gtggggccaa aactcttggc tcaacgcctc aacgtcaaag
     5341 aagacgaagt catcgagatg gagcagcgct tagccagccg agatctttcg gttgatgtgc
     5401 ccatcggcga cgatgatgag gcgacactgc tgcacttttt acccgatcat aagcagaatc
     5461 cggaagaaga atacgccgag tcgcagtatc agcagatgct gcgcgaaaag atggagcagt
     5521 tcgccaagac gttgaaggac aaggaactgg tgatttaccg ggaacgcttg ctcaacgaag
     5581 atccttcgac tctgcgcgaa atcggcgaca aatacggcat cagccgcgaa cgcgtacggc
     5641 aaatcgaaga acgcgttaag aaaaaattga agacctatct cagcaaggaa ctcaaagacg
     5701 ccacagatat cactggcgcc gattgatcat tgctctcgtt gagcgtcaac ctcgatcatt
     5761 tccgtgagca aaattgccgc gagttcgctt gcgcgaattg gcgccatttg atagcaaaat
     5821 tagatcgaac tttggaggaa cgtagttgca atttgaaaaa caagtggaaa tccacgtgcc
     5881 gcgggaaaag gtttggaaat ttatctggga tatcgaccgg tttatcgctt gtgtgccggg
     5941 gtgcaaagat gcgaaaaccg tggaagaagg taaacgttac accgcgacca tggtggagaa
     6001 ggttggacct tttaaggttg agtttcccac gaccatcgaa gtcttggagc gcgcagaact
     6061 tagccatatc aaagcgcagg cctcgggcgc cgacaacaag attggcagcc gcatgaaaat
     6121 cgatctcgac gtcaatctgc gcgagcagga taacacgacc gtcgtgagtt ttgtcgcggg
     6181 cgtcgatatt ctgggcaagc tcgctgcgct cggccatggg attatcaaac gtaaggctga
     6241 ccaagtgctt gatgagttcg cccaggcggt gaaaacgacc ttagagggag acaactgaga
     6301 tgctcaaacg gtttcgattg gaagaaccag aaagcgtcgc agacgtctcc gaactactcg
     6361 gccgatttgg cgatagcgcg aagatctacg ccggtggcac cgaacttttg ttggcgatga
     6421 aggaaggcct ggttcattac gaacggctga tcaacgtcaa aaacatcaaa ggtctcgggg
     6481 aagttgccgg cgacaatggc acgatcagaa tcggtgcttt atgcacccat cacgaattgg
     6541 aaaattcccc gttgttgcaa cagaaactgc cgtcctttgt taaactggag caaaacgtag
     6601 cgaatgtgcg tgttcgccag gctggttcca tcggcggcaa tatttgtttt gccgaacccc
     6661 acgctgatcc tggaacctta tttatcgcct tggatgcagt catggttgcc gagaagagtt
     6721 ccagcaagcg tgagattagt gccagtgatt tttttgtcga tgcctttgag acgtcgctgg
     6781 ccaccgatga gctgctcact gaaatccgca tacccacacc cggcgctaat cgccggagtg
     6841 cttatctaaa attcggctat ttggaaaggc caagtgtcgg tgtggccgtg gctttgacgt
     6901 tcgacagcgc cggcgtgaac gacgtgaaaa ttgctgtcgg ctgcgctggt cccgcaccaa
     6961 gacgtgtcac cgaagctgaa gccttgctta acggtaagag taaggacgag gcggtgagtc
     7021 tcttggcgca ggcaggggcc gtcgctggcc gggcatcgga ggcaatcagc gatctgcatg
     7081 gcgcgcagga ctataaagaa catattgttg gcgtgttact gaagcgcgcg tttaacagca
     7141 tcgtcagctg agaacctcat aataatttct acgttggaga tatacatgtc caaacagatg
     7201 acccgccatg ccgatacgca atcgatctct gtgtcgttta cgctcaatgg caggtcgcag
     7261 gaaatagaag tcgaacccca cgaattgctg ctcgatgtcg tacgggaccg attgggattg
     7321 accggcgcca aacgttcctg cgatgtgcaa gtgtgcggtg cctgtacctt attggtggac
     7381 ggccggccgg tgagtgcatg cacaaccctg gctttcgagg tacgtggtcg ttcggtgatg
     7441 acaattgagg gattggcgga ggacggtaag ctccatccgc tgcaaaatgc cttcatcgag
     7501 catggcggct ttcagtgcgg cttttgcacg cccggcatgc tgttggcctc gaaagcgcta
     7561 ctcgacgaaa atcccgaccc aagcgaagcc gagctgaagc actttatgca tggcaacctg
     7621 tgtcgctgca ccggttacaa gaagattatc gaatcgatta tggctgcggc taaagagatg
     7681 cggaaccgct agaactcgcc gcgcgagtaa tgcaattcga aattccagat cggctcttga
     7741 atatcttaaa tctgaaattc gtaatttgaa ccttccgtag cacgccggtt agtcctcgat
     7801 gccgtattct ttccagcggc ttgtaactag atcgaggtgt tctttaggcg gtagcgaaat
     7861 cgccggatac ttgtgtttgc gcgtggcgtc aattgctacg cgcgagccga cataacgact
     7921 cgcatgatgt tgcacgacgt cggccggtgc ctgcgacggg tcgagtccaa ccgcttgaac
     7981 gtcccgttca atataaacat ctttctccgg cctcacatgc cagctgagcg cccaatcgac
     8041 ggcgaagtcg tcccatacat cgatatcttc atcgaccacc acagtgatct tgccgaagcc
     8101 cgtgcgctgc gaccatgcgc cgtacatcag ctggcgcacc tggccgtcga actgtttttt
     8161 gagcgatatg accaaatacg cgccgctgcc gccggcttcc ttcaggcgca ggtccttgat
     8221 cggaagacgt aatatgtctt tcaaatgttt gaacagaggc cattcccgac cgacaccgcg
     8281 aatacaactg gattcagacg gcggcatctg actgatgaac gcttgataca gcggcttgtt
     8341 gcgaaaagtc atgcatttaa tgttgaaaaa cggctcattg ccggcggggc ccatgtagcc
     8401 ggtgtattca ccaaatgggc cttcatgttc gaaggcattg ggcggaatct cgccttcgag
     8461 gacgatctcc gcgctggcag gaacttccag cggtacggtt tcacaagcaa tcatgtcgac
     8521 tgcttcaccg cgtaaggctc cggcgaccgc gaactcatcg agtgcctcgg agatttttga
     8581 gaccgacaca taaccgattg atggatcggc gccgatgacg accgccagag gcgtcggctt
     8641 gttctgggcg ttgttcttgt acatatgcca cgccatgtct tggcgtttgc cgatcaacag
     8701 gccggttttg ttcggacctt ttacttgcat gcggtaggta ccgacattgc gcacgccggt
     8761 ctcgggatct ttagtgataa ggtacggcga ggtgaaaaac gggcccgggt cctcacctac
     8821 agtccacgtt ggcaccggca atttggtgat gtcgacttcc ttcccgtgca gaatgttttc
     8881 cttgcacggt gcatgcttaa ccaatctcgg ctcgatggga tgctcgagcg cctgatccca
     8941 tttgcggttg ataccttcga cggtctcggt ttgtagcccc aatgcataaa tttcgcgcga
     9001 ggcgccaagc acaccggcaa ccaccggaat gtcgaaaccc gcgacattct caaaaacgag
     9061 cgcaggacgt tgcgctggag ggattttctt gaacagttgc cggcaaacag cggcaatctc
     9121 ccagtctttg tcgacttttt tcgttacccg tttcaatttg ccatgctttt cgagcgccgc
     9181 gacatagtcg cggagatctt tgtacgccat gaaccctcca atcagaaaag aaaccgaacg
     9241 aaatttcttc gaatactaga cagaaatcgt caaagtatca acgattccaa tgtgaaatat
     9301 attggcgggc tagttgaagg cgacggcgaa tggagcttaa gcgcggatct cggcgcgcat
     9361 aaagattatg tatgaaatgc cgaagcaaac cgcgactaag gcgaacatcg tcgttatgtg
     9421 cggccagatc aggttcagac tttgaccgag ggcgagcggg gtgggtagaa tcccttcggc
     9481 ttggctctgt aacgccatgc cgaaaactcg tgcagcagga ttcaaaagaa tgccgacgct
     9541 ttcattgaac aacgtgctgg gtgaaacccg gccgacaata ttttcgatat cggatttgcg
     9601 ggcaaccgct tccggtgtgg tggcatcggg aacgaccatg ttcgcgatcg cgccgctgat
     9661 gactgcgacg aacagcgaga gaaacagcca gacggcaatg gaagcgaggg ctgccgtgac
     9721 agttttctga aacagcaaag aaaagagcat ggctaatgcc aaccaaaagc cgacataaaa
     9781 aattcccacc acggtaaaaa tgatcatccg ccagagttct tcggcattgg gtggaaagcc
     9841 taacagtgta atgcccaagc cgatcactag gagcaaaatc gagccccata acacaccgac
     9901 cgtggtcagg cccgcgaaga acttaccatt gatcaggctg tcgcggtaaa tcggctgaga
     9961 tagcacgcgg ctgagcgttc cgcgggcgta ttcaccgctg attgaatcga atcccaatgt
    10021 gatgccgacc agcggaccga agaatcccaa aaaggtcgcc aaagaaaata gcgtatcgcc
    10081 gctcgaggtt aacagcagta gaaacacgta ttgggtcggc acggtgtcgg tgttggcgcg
    10141 gatcgcctgt ccggtagcat aggcagccca cagtccggaa aagacgatca ggatcagcag
    10201 gatcatgaaa cgccggctgc taaagtggtc ggcaagctct ttccaaaaca ccacactcat
    10261 tttttacgcc tcgcggaaat atttgagata gatttcttcc aaggtcgggt cttcggagcg
    10321 aagctgcaat aagcttaatc cccggcgcg
//
LOCUS       DATLCH010000169         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1951017, whole genome shotgun sequence.
ACCESSION   DATLCH010000169 DATLCH010000000
VERSION     DATLCH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951017"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VMZ02_04980"
     CDS             complement(<1..191)
                     /locus_tag="VMZ02_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR71318.1"
                     /translation="MADKYDVIIVGGGSAGCVAATRLSEDSNRKVLLLEAGPDPWPLP
                     ELVAESKNQTRLLLESDYVA"
     gene            complement(325..777)
                     /locus_tag="VMZ02_04985"
     CDS             complement(325..777)
                     /locus_tag="VMZ02_04985"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HUR71319.1"
                     /translation="MILDGTIELNAPIEKAWDFLIDIKKFSTCLPGIDSVTQIDDKTF
                     DGIISATVGPISGKFNFRSTIVESKPPAQMVVKTEGQDTVTKSTVNADMTVDLRRVSD
                     TSSQMDYKADVKIKGRLGILGDMVLRATATLILQEFTKRLHQGLGAQA"
     gene            complement(1389..1946)
                     /locus_tag="VMZ02_04990"
     CDS             complement(1389..1946)
                     /locus_tag="VMZ02_04990"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF041052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HUR71320.1"
                     /translation="MAALPGTIDLGKFRVVYEEERQQPSDGTDGYLLQQRAVIRLNEE
                     LVRLEEPDDYTVYCDDDKFKQQHGKRPSPLQYFIASIGFCMFSQCKRLSAKAQVALDD
                     LEMELRMTYDLSGKFPIKDFSDAAQGMAYVFKIKSAAPLEKVLPVVRAADRGCHTVNS
                     MRKRVPVSGRFVLNEQLYELVDSMD"
     gene            complement(1971..>3772)
                     /locus_tag="VMZ02_04995"
     CDS             complement(1971..>3772)
                     /locus_tag="VMZ02_04995"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUR71321.1"
                     /translation="LKPSAKLLHPDVMNYKGLPNPLKEPTNDFVYVTWGKGDPEAGFR
                     QADVIVENTFTTPVVHQSYIEPHSCVVSTSLDGSAEFWSCSKVPYGVREQVANALKLS
                     QDKLNFNPVYIGGDFGGKGDFMDLAVVYLLSKKAGRPVKMVMDYEEEFIAGNPRHASI
                     IHVKTGVKKDGTLVAHRMSFIFDSGAYGAFKPNAFLNGPHLSAGPYKIPHVFIEEHMV
                     YTNKIPCGHMRSPGDPQGFFANESQLDIIAKKLGMDPIKLRQKNFMHDGDIDPTGEEY
                     SYIKTDETLKKALDDSGYQKTKPKNVGRGVALVQWTPAGGIGTVAISLDAKGVATISS
                     AMADQGAGTYTVLSEIIAEELKIPLSQVKVQQLDTQQGTKDTGVGGSRATRVYGNAGY
                     EAALKAVAALKQAAAEQMGTSPDQVVLAKGAALHPRMERRLSYGEIVKAKGAPIVVEA
                     TFNDNSKIHAASMCVQVAEVEVDPDTGRVELKKFTSTHNTGTVLNPLMHQGQIEGASM
                     TGIGYTLMEEVIINDGKVATTHFGEYKIPTIKDVPIFKGSVSEASHGAGPYDSMPIGE
                     TGNIPTAAAIANAVEDACGVRITSLPITAEKVY"
BASE COUNT          769 a         1072 c         1002 g          929 t
ORIGIN      
        1 gcgacataat cggactccaa caacaagcgc gtctgattct ttgactcggc aaccaactcc
       61 ggcagaggcc atggatcagg accggcttcg agcagcaaca ctttgcggtt tgaatcttcc
      121 gagagccgcg tcgccgccac gcagccggcc gaaccgcccc cgacaataat cacgtcatac
      181 ttgtccgcca tgaaaactcc tcattgatta ttcgaaattg ttgtcactaa ccgaaaagat
      241 ttctcgctac gttcgaaatg acagcttctc ggggttgtca tctcgaacgt agtgagagat
      301 ctatcgtctc acgccatact agagttacgc ctgcgcgcca agcccctgat gcaatcgctt
      361 ggtgaactct tgtaaaatca aggtcgccgt cgcgcgcaac accatgtcgc cgaggatgcc
      421 caatcgacct ttgattttga catcggcctt gtaatccatc tgcgaagagg tgtccgagac
      481 ccgccgcaga tcgacagtca tatcggcatt tacggtgctc ttcgtcacag tgtcttggcc
      541 ttcggtcttc actaccatct gcgccggcgg cttgctctcg acgatcgtcg aacgaaaatt
      601 gaattttccc gagatcggcc cgacggtggc ggagatgatc ccgtcgaagg ttttgtcatc
      661 tatctgtgtc actgaatcga ttccgggtaa gcaggtggag aactttttga tgtctatcaa
      721 aaagtcccaa gccttttcga tcggggcgtt cagctcgatg gtgccatcaa gaatcatcta
      781 ttgtttttgc tcctgccgta aattcaggcg cgatcttatg tgagaaaaaa atcgcgaaca
      841 aggggcaagc tcggggattt atcgacgccg gctatcttct tcctattagc gcgaaagcct
      901 ggagctgcgg aattagaatc gccagtctct tatgatcatt ttctgaatac gccgcaggca
      961 ttgcttaccg cccgccacgg cgcatgccgc attgaccgag gccacccgag taggctgctg
     1021 ctcctctgag tgcggggatc gacgacacag gtcactgcgc ggtaagcatc ccggcccagt
     1081 ggctttcttt tttgtgccgc agggtgcgga tcgctaagca gacgcaccgg gttgggatgg
     1141 agcgaaacat gcggccggtc ttcatgacag tcacgcgtca catgatcgat caggtttttg
     1201 cctccagcgg ttcgacacta atgccggagg atctgtacgt ttccctctag atgccgctcc
     1261 tgttcataca taccgtcgat atcgtttaaa atcgacgttg gctgccagtg cgatgtccat
     1321 taattccgtg aatgaaaatt gattccgtaa cgcgtacctg ggtacgggcg ccggcgcacc
     1381 ttacattgtc aatccatcga gtcgacgagc tcgtaaagct gttcattgag aacgaatctg
     1441 cccgacaccg gcacgcgttt acgcatcgaa ttgaccgtat ggcagcctct gtcggccgcc
     1501 cggacgaccg gtagcacttt ttccaagggc gcggcgctct tgattttaaa aacgtaggcc
     1561 attccctggg ctgcgtcgga gaaatctttg atgggaaact tgccgctcaa gtcgtaagtc
     1621 atgcgcaatt ccatctccag gtcatcgaga gcaacctgcg ccttggcgga aagccgcttg
     1681 cactgactaa acatgcaaaa accgatggaa gcgataaaat attgcagcgg cgacggccgc
     1741 ttgccgtgct gctgtttgaa cttgtcgtca tcgcagtaaa cggtgtaatc gtcgggttct
     1801 tcgagcctca ccagctcttc gttgaggcgg atgacggcgc gctgctgcag taagtagccg
     1861 tccgtgccat ccgacggctg ctgccgctct tcctcataaa cgacccgaaa ctttccgaga
     1921 tcgatggttc ccggaagagc tgccatgcat cacttcgcgc cgtgcagcgc ttagtaaact
     1981 ttctctgccg taatcggcag gctcgtgatg cgcacaccgc aggcgtcttc caccgcattg
     2041 gcaatggctg ccgccgtagg aatattgccg gtctcgccga tcggcatgct gtcatagggg
     2101 cctgctccgt gcgacgcttc cgaaaccgat cctttgaata tcggaacatc tttgatggtg
     2161 gggatcttgt actcgccgaa gtgggtcgtc gcgactttac catcgttgat gattacttct
     2221 tccatcaacg tgtagccgat gccggtcatg cttgcaccct cgatctgtcc ttggtgcatc
     2281 agcggattga gcacagtgcc ggtattatgc gtcgatgtga attttttgag ctccacccgc
     2341 ccggtatcgg ggtcgacttc tacttccgca acttggacac acatggatgc cgcgtggatc
     2401 ttggaattat cgttaaaagt cgcctccact acgatcggcg cccctttcgc cttgacgatc
     2461 tcaccgtagc tgagtcgtcg ttccatacgc ggatgcaaag ccgcgccttt ggccaacacg
     2521 acttgatccg gtgaagtgcc catctgctcg gccgccgcct gtttgagcgc agcaaccgcc
     2581 ttaagtgctg cctcatagcc ggcgttgccg tagacccgcg tcgcacggct gccaccgacg
     2641 cccgtgtctt tagttccttg ctgcgtgtcc aactgctgca ccttgacctg gctcaatgga
     2701 atcttcagtt cttcggcgat gatttcactc aatacggtat acgtgcccgc accctgatcg
     2761 gccattgcgg aggaaatcgt cgctacgcct tttgcatcga gcgaaatcgc aaccgtgcca
     2821 ataccgccgg cgggcgtcca ctgcaccagc gcgacaccgc gtccgacgtt tttgggcttg
     2881 gttttttgat agcccgagtc gtccagcgct ttcttgagcg tctcgtcggt cttaatgtaa
     2941 ctgtactcct cgcccgtcgg atcgatgtcg ccgtcgtgca tgaagttttt ttggcgcaac
     3001 ttgatcgggt ccatgcctaa tttcttggcg attatgtcca actgactctc attggcaaaa
     3061 aatccctggg ggtcgcccgg cgagcgcata tggccgcacg ggattttgtt ggtgtagacc
     3121 atatgttcct cgataaatac gtgaggaatc ttgtacggac cggcggacag atgcggaccg
     3181 ttgagaaagg cattcggctt aaaagcgcca taggcgccgc tgtcgaagat aaagctcatg
     3241 cggtgtgcga ccagcgtgcc atcttttttt acgccggttt tgacgtggat gattgacgca
     3301 tgccgcggat ttcccgcaat gaattcctct tcgtaatcca tcaccatctt gaccggtcgt
     3361 ccggctttct tggacaacag ataaaccacg gccagatcca tgaagtcgcc tttgccgccg
     3421 aagtcacccc cgatatagac agggttaaag ttgagtttgt cttgcgacag cttgagcgcg
     3481 ttggcgactt gttcgcgcac cccgtagggc actttgctgc acgaccaaaa ctccgccgag
     3541 ccgtcgagcg acgtgctgac gacgcaggaa tgcggctcga tataactctg gtgcactacc
     3601 ggcgtcgtaa atgtgttctc gacgattacg tccgcctgac gaaagccggc ttcagggtcg
     3661 cctttgcccc aagtgacgta gacaaaatcg ttggtcggct ccttgagtgg attgggcaac
     3721 cctttgtagt tcatgacgtc ggggtgaagg agtttcgcgc tgggcttaag gg
//
LOCUS       DATLCH010000170         4220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3225701, whole genome shotgun sequence.
ACCESSION   DATLCH010000170 DATLCH010000000
VERSION     DATLCH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3225701"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VMZ02_05000"
     CDS             <1..377
                     /locus_tag="VMZ02_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUR71322.1"
                     /translation="GEALSTGSGEGVGGSALGPGGRGGPGVVKGMEYVIYQNRMLSTI
                     KNNWVWVGPRSNIKVIVHFNIRDTGEIAGLKVVQSSGNSSYDESVLRAVRKSSPLPAL
                     TESIRNGFAEVELAFRPEDLEA"
     gene            382..1698
                     /gene="tolB"
                     /locus_tag="VMZ02_05005"
     CDS             382..1698
                     /gene="tolB"
                     /locus_tag="VMZ02_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HUR71323.1"
                     /translation="MKVLLLVFSLMVGFDIDCALAQFKGEIVGSGGQKFPIAVSPLRN
                     LGNAGGDTGRFSTGIADSIVHDLELSGWFRVIDRSSYIESPQASGITLGSFDFKDWST
                     LGAEGLVKGGFSLRGDEIVVELRLFDVYQGKERIAKRYTGGAGQFRRIAHKFVDEIIN
                     QFTGIPGVFNTQIAYVSNSGGRFKEIYYSHLDGSEKRQVTDNRTINLSPSWGANGRSI
                     LYSSFQDRGQTLYLRDLPSGKDIRFNPPGAGRFLSGKLSPDDQSIVATYETAGNSNLH
                     LLDRSGAIIRRLTQDPGIEVSPAWSPDGKQIVYVSDRSGSPQIFVLDLQSGKSRRVTY
                     SGSYNTSPEWSPRGDRIVYTGRVGSKFAIFTIPVEGGEPRKLTSEAFDSEDPTWSPDG
                     RFIAFSSNRAGKYHLYVMQASGENQRRLTGSGGDDSKPSWSPRLVD"
     gene            1740..2333
                     /gene="pal"
                     /locus_tag="VMZ02_05010"
     CDS             1740..2333
                     /gene="pal"
                     /locus_tag="VMZ02_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HUR71324.1"
                     /translation="MKFQSRTAYLGLLMIVSGAFGCAPSATTKPASSSSGSGSASAKS
                     PDSARPGDGQRGGTKESDLASSSLKSLQEGKPPITSASSPLKDAFFDFDRYDLSSEAR
                     AILQSNAEWLKSNGSTRVDIEGHCDERGTNEYNLALGAKRAQAARDYLVSLGIAADRL
                     STTSYGEEIPVCQERSEDCWRQNRRARFVIAERRPTT"
     gene            2359..3201
                     /gene="ybgF"
                     /locus_tag="VMZ02_05015"
     CDS             2359..3201
                     /gene="ybgF"
                     /locus_tag="VMZ02_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HUR71325.1"
                     /translation="MAHKLTFLLVAFGLAVTGCVNPQQVDLLEREQRRLRSDIGTVQT
                     NIDAARASLADARANMQQMQRDISEIRERIDETRVQVGRQIGQTSRDGDQRVKNLEGR
                     LVKLEEDAKAQAELLRIRESEIQKLKEAAQTQPSQPPPVSAPDGYAEAGLGESDGARR
                     DFESAWRLFERKDYRLAANRFREFLKKYPKSRIAANAQYWIGESHFGLKEYDKAIVEF
                     DEVRRRYPQSEKVPAALLRQGFAFAELGEKLNARLVLQDVVEKYPQSPESLSAKQKLK
                     SLES"
     gene            3300..>4220
                     /locus_tag="VMZ02_05020"
     CDS             3300..>4220
                     /locus_tag="VMZ02_05020"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HUR71326.1"
                     /translation="MQIIRHIDDPALALNDSSVTLGNFDGIHVGHQALIRNAVDDARR
                     MGLRSAVLTFEPHPLRVLAPERAPKMLLAHKDKMQLLQHLGVECVVIQQFDRSFAAID
                     AEGFVRKYLSERLRAKKVWVGKDLRFGKGRAGDIDKLRTWGTQLGFDVGVVDPVTVCD
                     ERVSSSRIRELIIAGQVAAVQPMLARYYFVSGKVISGQRRGRELGFPTANIASRAEIV
                     PRDGIYATLFHLGDNLLPSVTSIGVNPTFGAGGPRTIESYILNFNREIYGESVKVSFL
                     ARLRDEKHFASVELLIEQIRRDVVDAEALLA"
BASE COUNT          987 a         1034 c         1233 g          966 t
ORIGIN      
        1 aaggcgaagc gcttagtact ggttccggcg aaggcgtggg gggatcggcg ctggggccgg
       61 gcggtcgtgg tggtccgggc gtcgttaaag gcatggagta cgttatttac cagaaccgca
      121 tgctgagtac gattaaaaac aactgggtct gggtagggcc gcgcagcaat attaaagtca
      181 ttgtacattt taatatcagg gacaccggcg aaatcgccgg ccttaaagtt gtccagtcat
      241 cggggaactc gtcatacgac gaatcggtcc ttagagcagt gcgaaaatcg agcccgctgc
      301 cagctctaac ggaaagcata cgtaacggct ttgcagaggt ggaattagct tttcggccgg
      361 aggatctgga ggcgtgagtc gatgaaagtt ttgttgctgg tcttttcact tatggtcggc
      421 ttcgatatcg attgcgcgtt ggcgcagttt aaaggagaga tcgtcggctc tggcgggcaa
      481 aagtttccta tcgccgtatc gccattgcgt aatctcggta acgccggggg ggataccggt
      541 cgattctcaa cgggaattgc cgattctatc gtccacgacc ttgagttgtc gggttggttt
      601 cgggttatcg atcgatcgag ctatatagag agcccgcagg caagcggcat cacgctcgga
      661 tcattcgatt tcaaggattg gtcgacacta ggcgccgagg gcttggtgaa aggcggattt
      721 tctctgcgcg gcgacgagat cgttgttgag ttgagacttt tcgatgttta ccaaggcaag
      781 gaacgtattg ccaaacgcta taccggcggt gccggccaat tccgccgtat tgcccacaag
      841 ttcgtcgacg agatcatcaa ccaattcacc ggtattcctg gagtcttcaa tactcaaatt
      901 gcgtatgtat ccaacagcgg cgggcgcttc aaagagatct attattcgca cctcgacggc
      961 agtgaaaagc gccaggtgac cgataaccgg acgatcaatc tatcgccatc ttggggggcc
     1021 aacggccggt caattttgta ttcctctttt caggatcgcg gtcaaactct ctatcttcgg
     1081 gatctcccga gcggtaagga tattagattc aacccgcctg gtgcgggtcg ttttctcagt
     1141 ggcaagttgt ctcccgacga ccaatcgatc gttgccacct acgagaccgc cgggaacagc
     1201 aatctccact tgcttgatcg gagtggcgcg attatccggc gcttaaccca agaccctggg
     1261 attgaagtgt caccggcctg gtcgccagac ggaaaacaga tcgtctatgt gtcggaccgc
     1321 agcggttcac cgcagatttt cgttcttgat ctgcaaagcg gcaaaagccg gcgcgtgacc
     1381 tacagcggga gctacaatac gtcgcctgaa tggtcaccta ggggagatcg gattgtttat
     1441 accggtaggg tcggaagtaa atttgccatc tttacaatcc ccgttgaggg tggcgagccg
     1501 cgtaaattaa cttccgaggc atttgacagc gaagatccaa cctggtcgcc cgatggcaga
     1561 tttattgcct tctcgtccaa tcgggccggc aaatatcacc tctacgtaat gcaggcgagc
     1621 ggcgagaacc aacgccggct gacaggttct ggcggcgacg acagtaagcc aagctggtcg
     1681 cctcgtctcg tcgattgatt tcacaaatat acattttaac ctaaccatag gaggttgcga
     1741 tgaagttcca atctcggaca gcgtacctag gattgctgat gatcgtctca ggggcatttg
     1801 gctgcgcacc aagcgcgacg acaaagcctg cttcatcatc atctggaagc ggctccgcgt
     1861 cggcaaagtc accggacagt gctcggcctg gtgatggtca gcgtggcggc accaaagaat
     1921 ccgatctagc ttcgtcaagc ttgaaatcac tccaggaagg aaagccgccg atcacgtccg
     1981 catcgagccc gttaaaagat gcgtttttcg acttcgatcg atacgatctt agctcggagg
     2041 cgcgcgctat actgcagtcc aatgctgagt ggttaaaaag taatgggtcg acgagagtag
     2101 acattgaagg tcattgtgac gagcgcggca ccaatgagta taatctcgcg cttggtgcca
     2161 agagagctca ggcggcacga gattacctgg tttcgctagg aatagcggcg gatcgattgt
     2221 ccacgaccag ctatggagag gagattccgg tgtgccagga gcgttcggag gattgttggc
     2281 ggcagaatcg tcgtgcacgc ttcgtcatcg ctgagcgccg accaacgaca taacggagaa
     2341 ccaggaggaa ccccattggt ggctcataaa cttacatttt tattagtagc tttcgggttg
     2401 gctgttacag gttgcgtaaa cccgcagcag gtggatttgc tcgaacgaga acagcgtcgt
     2461 ttgcgcagtg atatcggcac agtgcaaacc aatatcgacg cggcgcgagc gagtttggcc
     2521 gatgccaggg cgaatatgca gcagatgcaa cgcgatatta gcgagattcg tgaacggatc
     2581 gatgagactc gagtgcaagt tggacggcaa atcggtcaaa ccagccggga tggcgatcaa
     2641 cgggtgaaaa atctagaggg ccgattggtc aaattggaag aggacgccaa ggctcaagct
     2701 gagcttttaa gaattcgcga gagtgaaatt caaaagttaa aagaggccgc gcaaacgcaa
     2761 ccatcgcaac cccctccagt gagcgcgccg gatggttatg cggaagctgg gttgggggaa
     2821 tcagatggag cgcggcgaga tttcgaaagc gcttggcgct tattcgagcg taaagactac
     2881 cggctggcgg caaatcgctt tagagaattt ctgaaaaaat atccgaagag ccggatcgct
     2941 gccaacgcgc aatactggat cggtgaaagc cattttggac tgaaggaata tgacaaggcg
     3001 atcgtcgagt ttgacgaagt tagacgccgt tatccgcagt cggagaaggt gccggcggca
     3061 cttctgcggc agggtttcgc gtttgccgag ctcggcgaga agttaaacgc gcgattggtg
     3121 cttcaagatg ttgtcgagaa atatccgcaa tctccagaat cgctcagtgc caagcagaaa
     3181 ctcaaatcgc tcgagtccta gaaaacattc ctcctcaggg tttgtccatt tcccggctat
     3241 tcctgggtgt tagcgtccgt gtgactcctt gagtggttcg gtgctattgt gccagcataa
     3301 tgcagataat tcgtcacatt gacgatcccg ctcttgctct caatgactct agcgtgaccc
     3361 tgggcaactt cgatggcatt cacgtaggcc atcaggcgct catcagaaat gccgtcgacg
     3421 acgcccgccg tatgggtcta cgttcggcag tactgacgtt cgagcctcac ccgctgcgag
     3481 ttctggcacc cgagcgggca cctaaaatgt tactggctca taaggacaag atgcagttgt
     3541 tgcaacacct tggcgtcgag tgcgtggtca tccagcaatt cgaccgctca tttgccgcaa
     3601 tcgacgcgga aggtttcgtg cgtaaatact tgagcgaacg gctgcgcgcc aaaaaagtct
     3661 gggtcggaaa agatttgcgc ttcggtaagg gacgcgcggg cgacatcgat aagctgcgaa
     3721 cctggggtac gcagctaggg ttcgatgtgg gtgtggtcga cccggttacc gtgtgcgatg
     3781 agcgtgtcag tagttcacga attcgtgaac tgatcattgc cggacaagtt gcggcggtac
     3841 agccgatgct ggcaaggtac tatttcgttt ccggcaaggt gatttcgggc cagcgtcgcg
     3901 ggcgagaact cggatttccc acggcaaaca ttgccagccg ggcggagatc gtgccgcgcg
     3961 atggtatcta cgcgacgcta tttcatcttg gtgataatct tctacctagc gtcactagca
     4021 ttggcgtaaa tccaacattc ggagccgggg gtccgcgcac aatcgagagt tatattttaa
     4081 atttcaatcg agagatctat ggcgagtcgg tgaaagtatc ttttctggct cggctgcgtg
     4141 acgaaaagca ttttgcgtct gtggaattac ttattgagca aatacgccga gacgtagtgg
     4201 acgcagaggc gttactcgct
//
LOCUS       DATLCH010000171         7310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4935662, whole genome shotgun sequence.
ACCESSION   DATLCH010000171 DATLCH010000000
VERSION     DATLCH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4935662"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..130
                     /locus_tag="VMZ02_05025"
                     /pseudo
     CDS             1..130
                     /locus_tag="VMZ02_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(88..408)
                     /locus_tag="VMZ02_05030"
     CDS             complement(88..408)
                     /locus_tag="VMZ02_05030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HUR71327.1"
                     /translation="MNAMSKQELESVAQALVAKGKGILAADESMGTIKRRFDSINIDS
                     NENNRRAYREMLFTTKGLEEAISGVILFDETLRTAASDGTPFSQVLTVIRLRTRASTS
                     ALVL"
     gene            503..856
                     /locus_tag="VMZ02_05035"
     CDS             503..856
                     /locus_tag="VMZ02_05035"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HUR71328.1"
                     /translation="MALVTVLHLLIHWAISAISLVVVAHLIPGIRVDGIGPALIAPLV
                     IGLVNATVGFIFKIVTFPITILTLGIFLLVVNAMMLMLAASLVPGFAVDGFWSAFFGA
                     IVMSIISMILRSLLT"
     gene            complement(910..1788)
                     /locus_tag="VMZ02_05040"
     CDS             complement(910..1788)
                     /locus_tag="VMZ02_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71329.1"
                     /translation="MDILSVTGYNRRPPHLVALYKGFFANENLEVRFHETTYAPDHNR
                     GMAEGRWDFTLSSADTMIARTTTDGTDYMLFMQAEEGLSAYLIAQPGYDSLDKLRGKL
                     LAGDPGDSNLDLIRKKILRKHGIDDGEYGIEIIGSSPVRLEAFLERRVVAAMLTPPAS
                     DKALAAGGILLASGDDYVPGWPLTCGWTLRPWLLEHREVVIRFIRAWAAATDWLLQAE
                     HKEESIQLSMDKEKLNRAAAEIAYSKVVPRARINPAAIQTVIELRKEMDVYKPPFAPP
                     ARFYDESYWREATGAN"
     gene            1863..2621
                     /locus_tag="VMZ02_05045"
     CDS             1863..2621
                     /locus_tag="VMZ02_05045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR71330.1"
                     /translation="MKKVTLEKCWQTWPMQKAPVKLKDKVALITGGGRGIGKAIAMHY
                     GREGAKLALCARTAAELDQTVKELRAVHIDAEGWTCDVSLEEPVQDFVARAHKKFGRI
                     DVLVNNAGIMTRPAPMSEMDVKKWDYTMAVNLRGPFLVTRAVLPIMMLQKSGSIINVS
                     STIGRGAYPNFVAYGTSKWGLEGFSQTLAAEARSSNIRVNSVEPGYVATKLTGFNGSK
                     PESVTDLFVYLASDESKAVTGKMLSTSGWQSRQK"
     gene            complement(2740..2919)
                     /locus_tag="VMZ02_05050"
                     /pseudo
     CDS             complement(2740..2919)
                     /locus_tag="VMZ02_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            3120..4940
                     /locus_tag="VMZ02_05055"
     CDS             3120..4940
                     /locus_tag="VMZ02_05055"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HUR71331.1"
                     /translation="MSRAVVSLILTALIGFATHPWELSGSEPPATDELSAHAKFLAAP
                     ELSGRGVGTPGIAAARDYIAAEFAKYGLLPGGDNDRYLQNFEVAVGVKVKSPTSLAID
                     RGPALTLAEDWLPLGLSGSGLVEGEAVFVGYGITAGEYGYDDYAGVNVKGKIVIILRY
                     EPPPKSALSPFRKSPDFSAHAALRTKANNARDHGAVGMILVDMDNRSGTQTELMSLRS
                     SLWRSGNSLIAVQIKRQAADNWLSSHGVSLGNLKDQIDGAEKPASLTIPGSKLTMQVS
                     LEEERAPAENVVGILPGREPLKGKEYIVIGAHYDHLGLGYYGVRDQSAAGMIHHGADD
                     NASGTSVLLHLAHRLSQVEPKPLRSIVFVAFSAEELGLHGSRRFVAAWPEIRSVKSMI
                     NLDMVGRMREQRVTIFGVRSSPQFETIIGSGAARLGLQTNLPDGVGPSDHLSFYNKQI
                     PVLHFFTGSHPDYHRPGDTWDKLNYAGMAQIGDLVLGSALAIAELPEAPKFVSLPTRL
                     PTRETGEARGSGVYLGIIPEYSKTVGGVLLAGVASDSPASAAGLHEGDVIIQLADRKI
                     DNIEDLTAALAAQKPAAQVTITVRRGEATNILNAVLGNRR"
     gene            5081..5689
                     /locus_tag="VMZ02_05060"
     CDS             5081..5689
                     /locus_tag="VMZ02_05060"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR71332.1"
                     /translation="MIKLYTFPPSTNSRKVRIALIEKGLEFERISIDLTKREQKNPDY
                     LKINPYGQIPALDDEGFIVYDSTIINEYLEDEYPYPALMPSDSEGRARARMMEDFRDT
                     QFNPYFVHIIQESRKPEGERDAQRIDTAKGEITKAFDRIESELQGNDYLTGTFSLADV
                     AFMANIDLLERFDIPVDPVKYKNTVSWIARLKARPSFAASSQ"
     gene            5770..6480
                     /locus_tag="VMZ02_05065"
     CDS             5770..6480
                     /locus_tag="VMZ02_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71333.1"
                     /translation="MANLIFAWLVHIYTALGAVLGLYTILAVEEANFRLAFLLMACTV
                     IIDATDGALARAARVKEIIPWFDGTLLDNLVDYLNYVIVPSLLLLRAHLLPEQDGLWL
                     AAMPVLSSAYGFCRSDAKTADHFFLGFPSYWNIVFFYLYALQTALWINAFVIIALSIL
                     VLVPVRFIYPSRSPSFRYQLNALGAIWGVAILYLIFKLPNPPRWLLLASLIFPAYYTA
                     LSLWLELRRVLAQDISPT"
     gene            6503..>7310
                     /locus_tag="VMZ02_05070"
     CDS             6503..>7310
                     /locus_tag="VMZ02_05070"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HUR71334.1"
                     /translation="MRKLRVCLYFIAVVYSVIPAPASSQIGRIANGAPKYVFFFLADG
                     AGMTHLEIARQYRQYIHNEGMVIVDKIIKEGTIGVITTHPANSLSTDSAAAATAMASG
                     CKARIGALGVCADGTPAVSAMEIARQRGMKLGLATNSQIYDASPAAFVCHVPNRKDYS
                     AILRRYQEMAPDVLLGGGMDQFLAKGQPGSQRGDDSDPVAEFTKLGYQTVNTLTQLQR
                     ASSNKVLGLFGLSEMSFEIDRDKQTEPSVYDLTQAAIRLLQGPRGFFAFIE"
BASE COUNT         1604 a         2065 c         1936 g         1705 t
ORIGIN      
        1 aattgccatc cgggccgggc accactgtgc gcaaccggtg atggagcgct tcggcgtccc
       61 ggccacggcg agagcatcgt ttgctttcta taatactagg gctgaggtcg acgccctggt
      121 tcgcagtctg atgactgtca agacctggga gaacggcgtg ccgtcactgg cggcggtgcg
      181 caatgtttcg tcgaacagga ttacgccgct aatcgcttct tcaaggccct tcgtggtgaa
      241 aagcatctca cgataggcgc ggcggttatt ctcgttggag tcgatgttaa tgctgtcgaa
      301 tcgtctcttg atcgtgccca tgctttcgtc ggcggctagg atccctttgc ctttggcgac
      361 cagggcttga gcgacgcttt cgagttcctg ctttgacatg gcgttcatgc ctccttaatg
      421 tgcgttggct cattttacat tctcgacgga gtcccgcaag agtgatccca aaaacctgtt
      481 tgagactaca cacggttgtg ctatggcctt agtcactgtg ttgcatttgc ttattcattg
      541 ggccatcagt gccatcagcc tcgttgtggt tgcgcacctg atcccgggga tccgcgtcga
      601 cggaatcggt ccagcgttga tcgcgccgct ggtgattggt ctggtcaacg ccaccgtcgg
      661 gttcattttc aagatcgtca ccttcccaat cacgattctg acgctgggaa tttttctctt
      721 agtggtcaat gccatgatgc taatgctcgc cgcctccctg gtcccgggat ttgcggtcga
      781 cggtttctgg tccgcctttt ttggcgcgat tgtcatgagt atcatcagca tgatcctgcg
      841 ctcgctgctg acctgagagg cgccgagact catacccggc agactttgtt gatttgcgtt
      901 ttcgtgtgac taattcgctc cggttgcctc gcgccagtag ctttcgtcgt aaaagcgcgc
      961 cggcggagca aatggcggct tgtacacgtc catctccttg cggagctcga tgacggtttg
     1021 gatggccgca ggattgatgc gcgccctcgg caccactttg gaataggcaa tttccgcggc
     1081 ggcgcgatta agtttttcct tgtccatcga cagctgtatc gattcttcct tatgctccgc
     1141 ttgcagcaac caatcagtcg cggccgccca agcgcgaata aaacgaatca ccacctcgcg
     1201 gtgttcgagc agccagggcc gcagcgtcca accgcaggtc agcggccaac ccggcacata
     1261 atcatccccg ctcgccaata gaattccccc tgcggccaaa gctttgtcgg aagccggcgg
     1321 tgtcaacatc gcggcgacaa cgcggcgctc gagaaatgcc tccaagcgca ccggcgagct
     1381 gccgatgatt tctatgccgt attcgccgtc gtcgatgccg tgcttgcgga gaatcttctt
     1441 gcgaatgaga tcgagattgg aatcgcccgg atcgccggcg agcaacttgc cgcgcagttt
     1501 gtcgagagaa tcgtaacccg gctgagcgat caagtaagcg ctcaatcctt cttcggcctg
     1561 catgaacagc atgtaatcgg taccgtcggt ggtggtgcgc gcgatcatcg tgtcggcact
     1621 gctcagcgta aaatcccagc gcccttcggc catgcctctg ttgtgatccg gcgcatacgt
     1681 ggtctcgtga aaccgaactt caaggttttc gttggcgaaa aaccctttgt acagcgcgac
     1741 caaatgcggc ggcctgcggt tgtatccggt gacactcaga atatccataa cgggattcct
     1801 ctctcggcgg tctttttcta tcgccttgca gctccagcct caagcccatc ttggtgtcgg
     1861 ttgtgaaaaa agttactctg gaaaaatgct ggcaaacttg gccgatgcag aaagcgcccg
     1921 tgaagcttaa agacaaagtt gctctaatta ccggcggcgg tcgtggcata ggcaaggcca
     1981 tcgccatgca ttacgggcgt gaaggcgcca aactcgcgct gtgcgcgcgc acggccgccg
     2041 aactcgatca aacggtcaaa gaattacgcg ccgttcatat cgatgccgaa ggttggacct
     2101 gcgatgtgtc gctcgaagaa ccggtgcaag acttcgtcgc ccgtgcgcac aagaaattcg
     2161 gccgcatcga cgtgctcgtc aataatgccg gaatcatgac ccggccggca ccgatgagcg
     2221 aaatggacgt caaaaaatgg gactatacta tggccgtcaa cctgcgcggt ccatttctcg
     2281 tcacgcgagc ggtcctgccg atcatgatgc tacaaaaaag cggctcgatc attaacgtct
     2341 cgtcaacgat cggccgcggc gcctatccga actttgtcgc gtatgggact tctaagtggg
     2401 gcttggaagg tttctcgcag acgcttgccg ccgaggcgcg ctcgtcgaat atccgggtca
     2461 attcggtcga gcccggttat gttgcgacca aactcacggg ttttaacggc agtaaaccgg
     2521 aatcggtaac cgacctgttc gtttatttgg cgtcagacga atcgaaagcg gttacgggga
     2581 aaatgctcag cacgtcgggc tggcagtcgc gccaaaaata ggttcttgaa acgctagata
     2641 agcgagcgtc gctcgacaga taaggtctcc agccagcttt ttcctcgggc gccaacagat
     2701 ctcaatcgag tcgcgcagca tcctatcact ctgctaaatt tacaatccga ggagctcgat
     2761 aattttcaat tgatcgaatc cgaccaccga ctgtccgtcg atgattgtca ccggcgtgct
     2821 ttgaaatcct tggtcgatta gctccttcat cgcagcctta tcggcacgaa catttttttc
     2881 gacgaattga acgccctttt gagatagaaa ctctacctct ttgtgacatg ggcctcagcc
     2941 cggttgggta tatacaacga cggaagtcgc catctcgata tcctccatgc gctagattga
     3001 tagtttataa cacttcacat ctgcgcccgc aaaagtcacg actgacgaca atgccgcatt
     3061 aacaatgtag attgagttac tgtatgcgac ctcattgcaa accgctattg aaccacgcct
     3121 tgtcgagagc cgtcgtcagt ttaattctga cggcacttat tggattcgcc acacatccgt
     3181 gggagctgtc gggttcggaa ccgccagcga cggacgagtt gtcggcccat gccaagttcc
     3241 tagcagcacc ggaactcagc ggccgcggcg ttggcacacc cgggatcgcc gccgcgcggg
     3301 attacatcgc cgcagaattc gccaaatacg gacttcttcc tggcggtgat aacgataggt
     3361 acctgcaaaa cttcgaggta gcggtgggcg tcaaggtaaa atcgcctact agtttggcta
     3421 ttgaccgagg gccggcgctg acgttggccg aagattggct gcctctaggt ctctccgggt
     3481 caggccttgt tgaaggggag gccgtgtttg tcggctatgg catcaccgcc ggggagtacg
     3541 gctacgacga ttatgccggc gtcaacgtca agggtaaaat cgtcataatc ctgcgctacg
     3601 agccaccgcc aaaatccgcc ctaagcccat ttaggaaatc acccgatttc tccgcccacg
     3661 ccgcgctgcg caccaaggct aacaacgctc gcgatcacgg cgcggtcggc atgattctcg
     3721 tcgacatgga taaccggagc gggacccaga ctgaactaat gtcgctgcgc agcagcctgt
     3781 ggcgaagcgg caacagcctg atcgcggtgc aaatcaaacg acaagccgcg gacaattggc
     3841 tttcgtcgca cggagtatca ctcggaaatt tgaaagacca gatcgacggc gcagaaaagc
     3901 cggcttcatt gacgataccc ggcagcaaat taacgatgca agtgagcctc gaggaagaac
     3961 gcgcgccggc tgagaatgtc gtcggtattc ttcccggcag agagccgtta aaaggcaagg
     4021 aatacattgt cattggcgcc cactacgacc atttgggtct cggttactac ggcgttcgcg
     4081 accaaagcgc cgcaggcatg attcatcacg gcgccgacga taacgcttcc ggcacctcgg
     4141 tgctgcttca tttggcgcac cgattgagcc aggtcgaacc taaaccgctt cgctccattg
     4201 tcttcgtcgc tttttcggcg gaggaactcg gattgcacgg ctcgcgccgt tttgtcgcgg
     4261 cttggccgga gatccgctct gttaaaagta tgatcaacct cgacatggtc ggacgcatgc
     4321 gcgaacaacg cgtaaccatt ttcggggtcc gctcgtcgcc gcaatttgaa accatcatcg
     4381 gcagcggtgc ggcacgattg ggattgcaaa ccaacttgcc cgacggcgtc ggcccgagcg
     4441 atcacctgtc attttacaat aagcagatcc ccgtgctgca tttcttcact ggcagtcatc
     4501 ccgactatca ccgcccggga gatacttggg ataaattgaa ttatgcgggg atggcgcaaa
     4561 tcggcgatct ggtactcggc tcggcgctag ccattgcgga acttcccgaa gcgcctaaat
     4621 tcgtcagcct gccaacacgc ctgccgactc gcgagaccgg agaggcgcgc ggcagcggcg
     4681 tttatctcgg aatcattccc gaatacagca aaactgttgg cggggtgctt ctcgccggcg
     4741 tggcttccga cagtcccgcc agcgccgccg gcttgcacga aggtgatgtg ataattcaac
     4801 ttgccgacag aaaaatagac aatatcgagg atcttaccgc cgcactggct gctcaaaagc
     4861 ccgctgctca agtgacaatt accgtgcgcc gcggcgaggc gacaaatatt ctgaacgccg
     4921 tgctcggcaa ccgccgttaa gacccgttcc agcaatgcag cgttattcgt tttgctagat
     4981 atctcggttt tttccgtcag aaaatgaaaa aaacgctcca cttattacaa cgttgtgcta
     5041 atatgtctct tgttgtgaag attaaggagg ttggctgtca atgatcaagc tttacacgtt
     5101 tccgccatcg actaactcgc gcaaagtgcg catagccctc atagagaagg ggcttgagtt
     5161 cgagcggatc agcatcgacc taaccaaacg cgagcaaaag aacccagact acctaaaaat
     5221 taacccttac ggtcaaattc cggcattaga tgatgaaggt ttcatcgttt atgattctac
     5281 aattattaat gaatacctgg aggacgaata tccgtatcca gcgctcatgc cgagcgactc
     5341 cgaaggtcgg gcacgtgcgc gcatgatgga agattttcgc gatacgcagt tcaatccata
     5401 cttcgtgcat ataattcaag agagccgcaa accagaagga gagcgcgacg cccagaggat
     5461 cgataccgcc aagggagaga tcaccaaagc gtttgatcgc atcgaatccg aattacaggg
     5521 caatgactat ctcaccggta cgttcagctt ggcagacgta gccttcatgg ctaatatcga
     5581 tctgctcgag cgcttcgata tccccgttga tccagttaaa tacaaaaata ccgttagctg
     5641 gattgctcgc ttaaaagccc gaccgagttt cgccgcatcg tcgcagtaac acgcaagcgg
     5701 tcagccagtg agaattgaag atagtcacac accgtcttca atccgacagg cccaaatcgt
     5761 aaactcgcca tggcgaattt aattttcgct tggctggttc acatctacac cgctctcggc
     5821 gcggtactcg gactctacac gattctcgcc gttgaggagg ctaactttcg tttagccttt
     5881 ttgctaatgg cctgcacggt catcatcgat gcaaccgacg gtgcgctcgc acgagcagcg
     5941 cgcgtaaaag aaattatccc gtggttcgac ggtactctgc tcgacaattt agtcgattac
     6001 ttgaattatg taattgtccc tagcctgctg ttgctgcgtg cgcatctttt gccggagcaa
     6061 gacggccttt ggctcgctgc gatgccggtg ctatcgagcg cctacggctt ttgccgaagc
     6121 gacgccaaaa ctgccgatca tttttttctt ggctttccgt cctactggaa catcgtattt
     6181 ttttatttgt atgcactcca aaccgcgctc tggatcaacg ccttcgtcat aattgctctg
     6241 tcaattctgg ttttggtgcc agtgcgattt atctatccca gccgttcgcc aagctttcgc
     6301 taccaactta acgccctcgg agcgatctgg ggtgtcgcaa ttctttacct cattttcaaa
     6361 ctgccgaatc cgccccgatg gcttttgctt gcctcgctga tctttcccgc ctactatacg
     6421 gccctgtcgc tatggcttga gctgcggcgc gttttggccc aagacatttc gccaacctaa
     6481 gactctggat caggagttct atgtgagaaa actgcgcgtt tgtctgtact tcatcgccgt
     6541 cgtttattct gtgattcctg ctcctgcatc gagtcagata ggacgcatag ccaatggtgc
     6601 gccaaagtac gtgttctttt tcctcgccga tggcgccggt atgacgcacc tggaaatcgc
     6661 ccgccagtac cgccaataca ttcacaatga gggcatggtt atcgtcgaca agatcattaa
     6721 agagggcact atcggcgtaa taaccaccca tcccgcgaac tcgctatcga ctgattcggc
     6781 ggcggcggca accgcgatgg ccagcggatg caaggctaga atcggcgcgt taggcgtctg
     6841 cgccgacggc acaccggcag tgagtgccat ggagatcgcc cgccaacggg gaatgaagct
     6901 gggtttggcg accaattcgc agatctacga cgccagcccc gcggcattcg tctgccatgt
     6961 gccaaaccga aaagactatt cggccatttt gcgacgctat caggaaatgg cacccgacgt
     7021 tttgttgggc ggcggcatgg accaatttct ggccaagggt caaccgggca gccaacgcgg
     7081 tgacgacagc gacccggtcg ccgaattcac aaagcttggt tatcagaccg tgaacaccct
     7141 aacccaactg caacgcgcca gcagcaataa agtacttggc cttttcggtc tttcagaaat
     7201 gagttttgag attgatcgcg acaagcaaac cgaaccgtcg gtttatgacc tgacgcaagc
     7261 ggcgattcgc ctgctacaag gaccgcgcgg ttttttcgcc tttatcgaat
//
LOCUS       DATLCH010000172         8057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4944289, whole genome shotgun sequence.
ACCESSION   DATLCH010000172 DATLCH010000000
VERSION     DATLCH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4944289"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..795
                     /locus_tag="VMZ02_05075"
     CDS             175..795
                     /locus_tag="VMZ02_05075"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HUR71335.1"
                     /translation="MLVHRRHFYQGIVALVLLVSCDGASGAEAVARKATVFGSPTANL
                     RAGAGVEHALKQALKEGDLLTVEESYGEWLLVSAADGQKGYIHKNLVRLEKDTAAPQV
                     TPPSTKSDEVPGRIVAPAGNTQAVPMRASDGPKPPSAITAVDSGARASAVTAGKAPSV
                     LQILESHEFEVKIALLAAGIAFVFGWFCGGHYYIRREHRHRRRLRL"
     gene            822..1406
                     /gene="plsY"
                     /locus_tag="VMZ02_05080"
     CDS             822..1406
                     /gene="plsY"
                     /locus_tag="VMZ02_05080"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HUR71336.1"
                     /translation="MQIFALIVFAYLLGSFPTGYVLGKFAGVDVRQVGSGNVGATNVA
                     RAVGKWQAVVTLIADAAKGMLPVAIGLWTNLQPAAIAAIAAAAFLGHLYPVFLRFRGG
                     KGVATAVGALLIIAPLATLLLVGLFVAVVIPTRLVSLSSMIAAAMAPLALWVFFQPPA
                     IVLLGAFLGAMIVWRHRGNIRRLCAGTEPRFGAH"
     gene            complement(1403..2110)
                     /locus_tag="VMZ02_05085"
     CDS             complement(1403..2110)
                     /locus_tag="VMZ02_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUR71337.1"
                     /translation="MKRTRPLAAATLLIAALISGCAGGELTTREKGAGIGALGGAAAG
                     GLIGAAVGRPGVGAAVGGALGLGGGALIGDQLQGQENRQKEQQKAIDQQRVELEKNRA
                     LIEELRKQNIEAKETSRGVTVNLPNVNFEFDSADLTRDGRDRVNQISSIIRRDAPNRR
                     VTVEGHASRESAAAEAYNQRLSERRAATVADALERNGVNGKNISSQGLGTRAPVASND
                     TENGRQQNRRVEVVIEN"
     gene            2174..2401
                     /locus_tag="VMZ02_05090"
     CDS             2174..2401
                     /locus_tag="VMZ02_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71338.1"
                     /translation="MDNALMKYSNGRFASVSARISTRLGDGFFRFLAFGVEVRPDSVD
                     LGLLIGLFIPVTLELIAYKAQALSLSAPPTS"
     gene            2593..3696
                     /locus_tag="VMZ02_05095"
     CDS             2593..3696
                     /locus_tag="VMZ02_05095"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HUR71339.1"
                     /translation="MENISQEKIIEALRNVQDPELHRDIVSLGMVKDLTIDNGRVRFT
                     VELTTPACPLRETIETDCRQALSVVPDITGLEINFGAQVRGSKAGAGQTDLLPSVKNV
                     VLVAAGKGGVGKSTVAANLAVALRILGAKTGLLDADIYGPSVPIIMGIKGEPKKIEVG
                     GVQKIEPAMAHGVPVMSIGFFLDADQAVIWRGPMLGKALHQLMGDVHWGELDYLIVDM
                     PPGTGDVQITFSQQLKVSGAVLVATPQYVALADVVRAKSMFDKVMIPIVGLVENMSYF
                     ICDGCNKEHDIFSRGGAKQAAEKFQIPFLGEIPITPALRQGGDEGVPILIQDANSPVS
                     KSFLDIARKLAGQLSIAAERGSKGQGPTIITTH"
     gene            3737..4036
                     /locus_tag="VMZ02_05100"
     CDS             3737..4036
                     /locus_tag="VMZ02_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HUR71340.1"
                     /translation="MITKEQVYEALHECYDPEIPVNIVDLGLVYDVEVIDDRVAVKMT
                     LTAPGCGMGGMIAADAKEKIMAIEGVNDATVDLVWEPAWNPSMIAEEAKQRLGIV"
     gene            4078..5328
                     /gene="amrB"
                     /locus_tag="VMZ02_05105"
     CDS             4078..5328
                     /gene="amrB"
                     /locus_tag="VMZ02_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HUR71341.1"
                     /translation="MGDKPRVRAIEAFPVQQDGKTLIYLKDPLNFATPLGISPVGYFL
                     LAHFDGRHDHRDLSEVFAQRFRAPLALDEFNRFVDMLDQHYYLKTERFDKYQQAVIAE
                     FRGRPTRPPAHVGGGYKNDPVDLAAQLDSYFAAPTGPGLPLNPNGETPPKAVVSPHID
                     FHRGGPAYAWAYKPLLESAGADLYVVLGTSHCGGQTPYILTRKSFETPLGLVETDGDF
                     VEQLQSNCAEDCFADEYLHRGEHSIEFQVLFLKYIAQKRAALTGNPEKPFKIVPILVS
                     SFHAMMVSQTLPEETLAVGNFLRTLRRLANDDGRQICFVAGVDLAHVGKQFGDEEPVT
                     EDFLKWVEAEDRQLVDRLARLDAAGFFNEIAKDEDKRKICGFSPLYSLIHLVDGAHAK
                     QLHYGQAFTPETGSAVTFTSLIFE"
     gene            complement(5413..6171)
                     /locus_tag="VMZ02_05110"
     CDS             complement(5413..6171)
                     /locus_tag="VMZ02_05110"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HUR71342.1"
                     /translation="MRLKDKVAIVTGGGIGIGKAYAHGLAKEGAKVVVADIQDAEAQK
                     VAADIKAAGGEAIAVWVDVTSPEKTQAMAAAALQAYGRIDILVNNAGLYSALKKKAFM
                     EIDTDEWDKVMAVNVKGLFLCVKAVYPAMKRQQKGKIINISSGTTQTGTPFFLHYVSS
                     KGGVLSFTRALAREVGGDNICVNSIMPGLTISSPNQEGVTNPEQLADRRKRRALQRDQ
                     YPQDLVGTVIFLASDDSDFMTGQSIVVDGGMNMH"
     gene            complement(6230..6988)
                     /locus_tag="VMZ02_05115"
     CDS             complement(6230..6988)
                     /locus_tag="VMZ02_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71343.1"
                     /translation="MIAGLKDRVVIVTGGAHGIGKAYCKGFADSGARIVIADIDKVGA
                     DAAAREIGAQALPIQVDVSEEEATKKMAAQVLDRFGGIDVLINNAAIFSVVPMNRGTI
                     ETIEPQEWDKLMAVNLRGLFFCCRAVLPTMRRQKSGKIINIASGTVFAGSPGRIHYVT
                     SKSATIGFTRTLAREVGGDNINVNCLAPGNTLSEENPTQEIIHLRQSSAGLRALKRVQ
                     VPQDVVGAMLFLASSLSDFMTGQTINVDGGIAFL"
     gene            complement(7104..>8057)
                     /locus_tag="VMZ02_05120"
     CDS             complement(7104..>8057)
                     /locus_tag="VMZ02_05120"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUR71344.1"
                     /translation="HGKLKMDYPIILGHEILGRIEKLGKHAATIHHVKEGDRVILKGA
                     LGCGRCADCRRNNQRFCRQRTNYGGRTKCDQPPHLFGGFADYLYLAPDVLCTKVSESL
                     SAEAAALVGSVMANGFQWAIRRGGVKMGDYVLIQGPGQQGLSCTFASKHAGAAKIFVT
                     GMGRDKPRLDLAKKFGATRVINVEEEDVVDVVRKETDGELCDVVVDVSGNPNAILKSV
                     DCLHRQSTMVLGGLTGDTTVTPMLMDKLVWNEIKLQGCFTADNDATEAALRIIEATKF
                     PVEQMVSHIFPLKDIEHCIRSIGGEVPGTFPTKALINPAIG"
BASE COUNT         1669 a         2184 c         2297 g         1907 t
ORIGIN      
        1 ggccaagtcc ggcacgtcga gcaagatgga tcctaatttt tacaaaagac cgtaaggtcc
       61 gctaattctg ctttacaaaa gccccgccgg gacctctctg cggggctttt tgcgtctgat
      121 gcctttgcag aaatttcatt gagcggcaat ccgcggttgt gttagattgc cgacatgttg
      181 gttcatcgtc gccactttta ccaaggcatc gtcgcgctcg tgttgttggt tagttgtgac
      241 ggcgcctctg gcgcggaggc agttgcgcgt aaggccacgg tgttcgggag tcctacggct
      301 aatttgcgcg ctggcgcggg agtggagcat gcactcaagc aggctctgaa agaaggggat
      361 ttactgacgg tcgaagaatc atacggcgag tggttgctgg tcagcgccgc ggatggccaa
      421 aagggatata ttcataagaa tctagtgaga ctcgagaaag acactgcggc accgcaggta
      481 acgccgccgt cgacgaagag cgacgaggtc cctgggcgca ttgtagcgcc tgccgggaat
      541 acccaagccg ttccaatgcg tgctagtgac ggccctaagc cgccatcagc gataaccgca
      601 gtcgactccg gcgcaagagc cagcgctgtg acggccggca aagcaccatc agtactgcaa
      661 atactcgaat cccatgaatt cgaagtaaag attgccttgc tggccgcagg aattgcgttc
      721 gtgttcggtt ggttttgcgg cggtcattac tacatccggc gcgagcatag acatcgccgg
      781 cggctccgtt tgtaatcgcg gtgcggccgc atcggattac tatgcagatc tttgcgctaa
      841 tcgttttcgc ctacttactc ggttcatttc ctaccggcta tgttttgggt aaattcgccg
      901 gcgtcgatgt gcgacaggtc ggcagcggta acgtcggtgc gaccaacgtc gcgcgcgcgg
      961 tcggcaaatg gcaggctgtc gttacgttga ttgccgacgc cgctaaggga atgctgccgg
     1021 tggctattgg tctatggacg aatctgcagc cggcggcgat cgcagcgatc gccgccgcgg
     1081 cattcctcgg tcatctctat ccggttttct tgaggtttcg cggcgggaag ggagtcgcaa
     1141 cagcggttgg agcgttgttg atcatcgcgc cgctggcgac attgttgctg gtggggttgt
     1201 tcgttgcggt ggttatcccg acgcgcttgg tttcgttgag ttcgatgatt gccgccgcga
     1261 tggcgccatt ggcgctgtgg gtattttttc aacctcctgc gattgttcta ttgggcgctt
     1321 ttctcggcgc gatgatcgtc tggcgtcatc gcggcaatat ccgtcgttta tgcgcgggta
     1381 ccgagccgcg tttcggcgca cattaatttt cgataactac ttcaacccgt cggttctgtt
     1441 ggcgcccgtt ctcggtgtcg ttacttgcta ctggagcgcg agtgccaagg ccctgagatg
     1501 agatgttttt gccattcacg ccgtttctct ccagcgcatc ggcgaccgtg gccgcgcgtc
     1561 gttcggataa gcgttggtta taagcttccg cggccgcgct ctcccgactg gcatgccctt
     1621 cgacggtaac ccggcgattc ggtgcatcac ggcgaataat gctcgagatc tgattgacac
     1681 ggtcgcgacc gtcgcgtgtc agatcggcac tgtcgaactc aaagttgaca ttgggcaaat
     1741 tcaccgttac accgcggctg gtttctttcg cctcgatatt ttgtttgcgc agttcttcaa
     1801 taagcgcgcg gttcttctcg agttcgacgc gctgctggtc gatggctttc tgctgttcct
     1861 tttggcgatt ttcctgccct tggagctggt cgccgatcaa ggcgccaccg cctaatccca
     1921 gtgcgccacc gacggccgcg ccaactcccg gccggccgac tgcagcgccg atcaatccac
     1981 ctgcggcggc gccgccgagc gcgccaatgc cggcgccttt ttctcgggta gtcaactcac
     2041 ccccggcgca cccagatatc aaagctgcga tcaataacgt agctgcggct agtggtctcg
     2101 tacgtttcat aattcctccc ggcctatgct ggatattctt ggccgtctct gtttagacgt
     2161 attgctaccc taaatggata acgcgctgat gaaatattcg aatgggcgat tcgcttcggt
     2221 ttcggctcga atcagtactc gccttggcga cgggtttttt cgatttctcg cttttggcgt
     2281 tgaagttcgg cctgattctg tcgatctggg gctgctgatc ggcttgttta ttcccgtgac
     2341 cttggagctg attgcctaca aggcacaggc gctgagcctt agtgcgccac ctacgtcctg
     2401 aaaagattag tgaatcgcag caactgctta taaatattag caaagagggg caaaagcagc
     2461 aagaaagaaa atcccgaacg tcgaaccatc ttgcgttata tcggaagcat ctatgattgc
     2521 actgtaaaag cgagcgcaat attgacatcg tagaccttat aagtctatag tttttcgtcc
     2581 gagaggagcc gcgtggaaaa tatttcgcag gaaaaaatca tcgaagcatt gcgcaacgtc
     2641 caagatccgg aactgcatcg tgatatcgtc agtctcggga tggtaaaaga tctcaccatc
     2701 gacaacgggc gagtgcgttt taccgtagag ctgacaacgc ccgcctgtcc cttgcgcgaa
     2761 accatcgaaa ccgattgtcg gcaggcattg agtgttgtcc ccgacatcac cggcttggag
     2821 atcaatttcg gcgcgcaggt gcgcggcagc aaggccggag caggtcaaac tgatttactg
     2881 cctagcgtca aaaatgttgt tctggtcgcg gcgggaaaag gcggcgtcgg caagtcaacg
     2941 gtggcggcga atctcgctgt ggcgctcaga atcctcggtg ctaaaaccgg cttacttgac
     3001 gccgatattt atggaccgtc agtgccgatt atcatgggta tcaaaggcga accgaaaaaa
     3061 atcgaagtcg gtggcgtgca aaagatcgaa ccggcaatgg cccatggtgt gccggtaatg
     3121 tccatcggtt ttttcctcga cgccgatcag gcggtgattt ggcgcggacc gatgcttggc
     3181 aaagcgctgc atcaactaat gggcgacgtc cactggggcg aattggatta tctaattgtc
     3241 gatatgcctc cgggcactgg cgatgtgcaa attacctttt cgcaacaact aaaagtgagt
     3301 ggcgccgttt tggttgcgac gccgcagtat gttgccttgg cggacgtggt tcgggcgaag
     3361 tcgatgttcg acaaagtgat gatcccgatc gtcggcttgg tcgagaacat gagctatttc
     3421 atttgcgacg ggtgcaacaa agagcatgat attttttccc gcggcggcgc caagcaagcg
     3481 gcggagaagt ttcagatccc cttccttggc gagattccga tcacgccggc gctacgccaa
     3541 ggcggcgatg aaggggtgcc gatcttaatt caggatgcca attcaccagt aagcaaaagc
     3601 ttcttggaca tcgctcgcaa gctggcggga caactgtcga tcgccgctga gcggggcagt
     3661 aagggccaag ggccaacaat cattacgacg cactaaatcg cccatccgag cctactcgca
     3721 gcgaggagac ctgattatga ttaccaaaga acaagtgtac gaagctttgc acgaatgtta
     3781 cgatccggag attccggtga acatagttga cctcggcctg gtttacgacg tcgaggtgat
     3841 cgacgaccgg gtggccgtca agatgacact gaccgcgccg ggttgcggga tgggcgggat
     3901 gatcgctgcc gacgccaaag aaaagatcat ggctattgag ggcgtcaacg acgcaacggt
     3961 tgatctagtt tgggaacctg cgtggaatcc gagcatgatc gccgaagaag ccaagcagcg
     4021 tttaggcatt gtctaaagca cacttcatca ctcatctctg tatctgatcg cgtgtccatg
     4081 ggcgacaaac cgagagttcg tgctatcgaa gcttttcccg tgcagcagga tgggaaaacc
     4141 ctcatttatc tcaaagatcc gctcaacttc gccacgcccc tgggaatttc cccggtcggt
     4201 tatttccttt tagctcattt tgacggccgc catgatcacc gcgatctcag cgaagttttc
     4261 gcgcaacggt ttcgcgcgcc gctggcgctc gatgagttca atcgcttcgt ggatatgctc
     4321 gatcagcatt attatttgaa aaccgagcgg tttgacaaat accagcaagc cgtcattgct
     4381 gagtttcgcg gccggcccac ccgcccaccg gctcatgtcg gcggcggcta taagaacgat
     4441 ccggtcgatt tggccgcgca actggacagc tactttgccg cgcccaccgg tccggggctg
     4501 ccattgaatc ctaatggcga gacgccgccg aaggcggttg tctcgccaca catcgatttc
     4561 caccgcggcg gaccggctta tgcttgggcg tataaaccac ttctggaaag cgccggcgcc
     4621 gatctttacg tagtcctcgg cacttcacac tgtggcggac agacgccata catcctcacg
     4681 cggaaaagtt ttgagactcc gcttggtttg gtcgaaaccg acggcgactt cgtcgagcag
     4741 ttgcagtcca actgtgctga ggactgcttc gccgacgagt atctgcatcg gggcgaacat
     4801 tccattgaat ttcaggtgct gtttctgaag tatattgcgc agaagcgcgc cgcgcttacg
     4861 gggaacccgg agaagccgtt taaaattgtg ccgatcttgg tttcgtcatt tcacgcgatg
     4921 atggtcagcc agacgctgcc ggaagaaacc cttgcggtgg gcaatttttt gcggacactg
     4981 cgccggttgg caaatgacga tggacggcaa atctgtttcg ttgccggtgt cgatctagct
     5041 cacgtcggca aacagtttgg tgacgaggag ccggtcaccg aggatttcct caagtgggtc
     5101 gaggcggaag atcgtcaact cgtcgaccgg ttggcgcggc tcgacgcggc gggatttttt
     5161 aatgagatcg ccaaagacga ggacaagcgc aagatctgcg gcttctcacc cctctattca
     5221 ctgattcatt tagttgacgg tgcccacgcc aaacagcttc attatggtca ggcatttacg
     5281 ccggagaccg gttctgcggt gacgtttacc agccttatat tcgaatagac ccaatcgaga
     5341 gtcgagacag agaccgccca actcccgagc acaggtcccg gactatttct tctatctgca
     5401 cgctcgatct cctcagtgca tattcatacc gccgtcgacg acgatggatt ggccggtcat
     5461 gaagtcgctg tcgtcggagg caagaaagat cacggtgccg actaagtcct gtgggtactg
     5521 atcgcgctgg agggcgcggc gctttctgcg gtcggccagc tgttccggat tggtcacgcc
     5581 ttcttggttt ggactggaga tcgtcaagcc gggcatgatc gagttcacgc agatattatc
     5641 gccaccaact tcccgcgcca acgctcgggt gaagctcaag acaccgcctt tggatgatac
     5701 gtaatgcagg aaaaagggcg tgccggtctg agttgtaccc gatgagatgt tgatgatttt
     5761 tcctttttgc tgtcgtttca ttgccgggta gaccgctttc acacaaagga aaagtccttt
     5821 gacgttgacc gccatgacct tatcccattc atcggtatcg atttccatga aggctttctt
     5881 cttgagcgcg gagtagagcc cggcattgtt gacgaggatg tcgatccggc cataagcttg
     5941 caacgcggcc gcggccatcg cttgagtttt ttccggcgag gtgacatcga cccatacggc
     6001 gatcgcttcg cctccggcag ctttgatatc ggcggcgacc ttttgagctt ccgcatcttg
     6061 aatgtcagca acgacaacct ttgcaccctc cttcgccagg ccgtgtgcat aagccttgcc
     6121 gatgccgatg ccgccgcctg tgacgatcgc tactttatcc ttcaaacgca tggtgttcct
     6181 ccgttagatt agagaactga taaaacgaat agattcacgt cggtatgcgt cacaaaaacg
     6241 ctatgccgcc gtcgacattt atcgtctggc cggtcataaa atcgctgagc gatgatgcga
     6301 ggaatagcat ggcgccgacg acgtcctggg gaacctggac gcgcttgagt gcgcgcagcc
     6361 cggcggacga ctgacgcagg tgaattattt cttgagtggg attttcctcc gaaagggtat
     6421 tgccgggcgc caggcaattc acgttaatat tgtcgccgcc gacctcgcgc gcaagcgtcc
     6481 gcgtgaagcc gatggtggcg gattttgacg tgacatagtg tatgcgtccg ggactgccgg
     6541 cgaaaacggt gccagaggcg atgttgatga tcttgccgga tttttgccgg cgcatggtag
     6601 gcaagaccgc gcgacagcaa aaaaataggc cgcgtagatt gactgccatg agtttgtccc
     6661 attcttgcgg ctctatggtt tcaatcgtgc ctcggttcat cggcacgact gaaaatatcg
     6721 ccgcgttatt gatcagaaca tcgatgccgc cgaaacgatc gagaacctgc gcggccattt
     6781 tcttggtcgc ctcttcttcc gacacatcga cttgaatggg cagagcctgc gcaccgattt
     6841 cgcgggctgc ggcgtcggcg ccgaccttat cgatatcggc gattacgatt cgcgcgccgc
     6901 tgtcggcgaa gcctttgcaa taagctttgc cgatcccgtg ggcgccgccg gtgacaatga
     6961 ccacccgatc tttcagtccg gcaatcatct cagacgtgaa gtagatgaag gattggaaag
     7021 atgcttaatc gtgtgttgga agaagtggcg gaaatcttcc gccgctattc cattactcca
     7081 acactctatc gctccgttca ccctcacccg attgccggat tgatcagcgc tttggtcggg
     7141 aaagttccgg gcacttcgcc accgatcgag cggatgcagt gctcgatgtc cttcaaagga
     7201 aagatgtggc tgaccatctg ctcgaccgga aacttggttg cttcgatgat tcgcagtgcg
     7261 gcttcggtgg catcgttgtc ggcggtgaag cagccttgca gtttgatttc gttccagacc
     7321 aatttgtcca tcaacatcgg tgttacggtg gtatcgccgg tcaaaccgcc cagcaccata
     7381 gttgactggc ggtgcaggca gtcaaccgat tttaagatcg cgttggggtt gcccgaaacg
     7441 tcgacgacga cgtcgcatag ctcgccatcg gtttccttgc gaacgacatc gacaacatct
     7501 tcttcctcga cattgatgac gcgcgtcgcg ccgaattttt ttgcgagatc gaggcgcggt
     7561 ttgtcacggc ccatgccggt gacgaaaatt ttcgccgcgc cggcgtgttt agacgcgaag
     7621 gtgcaggaaa gtccttgctg gccgggccct tgaatcagca cataatcacc catcttgacg
     7681 ccgccgcggc gaatcgccca ctgaaagccg ttggccatga ccgagccgac cagcgccgcc
     7741 gcttcagccg agagcgattc actgacttta gtgcacagaa catcaggcgc aaggtaaaga
     7801 taatccgcaa agccgccgaa caaatgcggc ggctgatcac atttggttcg gccgccatag
     7861 ttggtgcgct gacggcagaa acgttggtta ttgcgccgac aatcagcgca gcggccacaa
     7921 ccgagagcgc ctttgagaat gacgcgatcg ccttctttga cgtggtgaat cgtcgcggca
     7981 tgcttgccga gcttttcgat ccggccgaga atctcatggc cgagaataat cggataatcc
     8041 atcttcagtt taccgtg
//
LOCUS       DATLCH010000173         8413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4948094, whole genome shotgun sequence.
ACCESSION   DATLCH010000173 DATLCH010000000
VERSION     DATLCH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4948094"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..1777)
                     /locus_tag="VMZ02_05125"
     CDS             complement(434..1777)
                     /locus_tag="VMZ02_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUR71345.1"
                     /translation="MASKILIVDDEPFNLDLLEQELDELGFASERANNGAQALQKIAR
                     GNPALVLLDYQMPDMNGIEVLRNIRQTDKDLPVVIITAHGSIERAVEAIKAGADDFIT
                     KPFDPEHLAVVIKKALDRAQLKQDVEFFAQEFGGRNWLVAGTSAAMRGIVEEAKKAAT
                     SKSTVLLLGESGTGKELFARSIHDWSERRLKPFVAINCAGLAKELLESDLFGHEKGAF
                     TGAHQLKKGKLEFAHGGTVFLDEIGDLALELQTKLLRVLQEREFERVGGTAKISVDLR
                     IIAATNRDLETAVTAATFRADLYHRLNVIPLTLPPLRERQEDISALANYFLQRCAADT
                     KKKFSTIASDAEEKLLAYSWPGNVRELANVIERAVVLGDGPQLTLHHLPARVIGAQPP
                     GRSEETSFHDAINTYRRELIVRALANSQGNRANAAKALGMHRTHFMKLLKALQIT"
     gene            complement(1787..4426)
                     /locus_tag="VMZ02_05130"
     CDS             complement(1787..4426)
                     /locus_tag="VMZ02_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HUR71346.1"
                     /translation="MEPLADTIRGISIFSGLTREDVAKVMGKMEELNLAAGATIVRQG
                     DRGDAFYLIQSGAVQVVVDSGAGNSEIVAILGPQDCFGEMALFSGEPRSATIVTIKDS
                     ILWRLSRQDWDELIARYPTWLMQLCATLSKRLSFVDRQYSSGREAFNSLAEEFYAGRS
                     PRQQEFFRHASLLHAIDSATIDQLFQSKSVGRVVADLIKGQSPLIRRLGNDRIEFHSF
                     FKDFLHDKLLTIDGSDTQQRLHGDFAKRYVAIGNWQQAVRHAAEVPDWPEVIKLVKAQ
                     SKETLVEAAPVVKNILERIPREYFIADTALVNLKSDILIHLGDLPGAFRAYQEILAQR
                     AAAGEAIASYRRIAETLARRKEYVQAIGQLRSALGLVERDMTLPEHYSDVAPEPVSMS
                     FNRQLSSADAPRRWRFSAAALQSAPLSKWFGALLGLSVGGYLWFATPDIGLEPSGTKL
                     LGLISLTLIFWVFRTLPDYGVALVFAMAVILTKLETASTVMGGFASTTWFMTLGVLGL
                     GAAITGCGLFYRLSLHLVRLFPLNFHWQIVATGIMGVVVMALIPQQTARTVITSQMLI
                     NLSDSLGYKTPSKASTGLFVASFLGLGQLGFLFLTGSTTSLIAWGLLPADVREQFSWG
                     YWFLAALPPTLVVIIIILAVTIFLYRPETQSQISYKMVQTQLDILGPLSHKEWISLIV
                     LLLTVGGWLTAPYHGIDGAWISIIAFSVLVNTSILDWGMMRKGIDWELLIHMGVTLSL
                     PTLMKQAKIDQWLVDVISPLILPFMDSPAWFFIVIALLTYLLKLVFTSFLAVVTLCVA
                     LLPLSIDVGMNPWIIAMMILIASEVWFFPYQVDWHMLAFSTTDGKGFSYPLMCRINPV
                     YAIAYIVALLLAIPYWRFLGLMN"
     gene            complement(4434..5432)
                     /locus_tag="VMZ02_05135"
     CDS             complement(4434..5432)
                     /locus_tag="VMZ02_05135"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71347.1"
                     /translation="MVKLISLRKSILQAITLLFFMSAGAVTQGRTQTHARLGVLLPEL
                     GRPQSQSLKGLTDVLKSLGYIERKNLVIETRNAKGDRGALPRAADDLVARKVDVIFTT
                     GTRATTAAAGVTGEIPIVFVHPGDPVAAGLVKSMTDPQRNLTGVAGFAVEKTEKRLAL
                     LKELIPELRELHILFDSNNSFSRDNVALAQAAAKKISLPVVEHGVKSADELKTTLTSM
                     RPVPGAALFHIPDDLVEGEAEFVFTTARQKKLPSMFNDESWAIQGATAAYGPSNLDMG
                     RRAGAMIGRILKNPKNPLPPIERADKFDFIINYRTATFIGMQVSPAVLKRADKIIR"
     gene            complement(5432..6355)
                     /locus_tag="VMZ02_05140"
     CDS             complement(5432..6355)
                     /locus_tag="VMZ02_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR71348.1"
                     /translation="MAAMTLGPSDFRVRFWGVRGSYPTTGPSTSEIGGDTSCVEISVA
                     GRRLIFDAGTGIIPLGKNICRRAASRPTSYVFLSHTHIDHLMGLYFFEPLLTAGARSF
                     IYGPGRSNGALQQTLRQLTQNNLFPVTFDDLRGNIEVHSLGGGETLEFAAPGERLAVR
                     RSPPSRNQANGAASIVTHKSPAHPRDGVMLYRIKYRGKTLVYATDVEQQAGGYADIID
                     FARGADMLIHDAQYLHGEYYSKTKSKAGWGHSTIEMAAEVAVKAQVKQLVLFHHEPQH
                     DDAAMRRIARFSKTLFPNAIVAYQGLELKLL"
     gene            6790..7035
                     /locus_tag="VMZ02_05145"
     CDS             6790..7035
                     /locus_tag="VMZ02_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HUR71349.1"
                     /translation="MTVDKEFFNVKYKEGSLDAKTAQLVFFAVSVAIGHEDGAKLHLA
                     KARECGATEDEITEAVVYAMRPAAAKVRNFAKAVIGK"
     gene            7100..7954
                     /locus_tag="VMZ02_05150"
     CDS             7100..7954
                     /locus_tag="VMZ02_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HUR71350.1"
                     /translation="MSQWIIDVNEENFEAAVLERSSEVPVLVDFWAEWCGPCKALGPV
                     LEKLADEYAGGFVLARLNVDENPNLAGMFGIQGIPAVKLFRDGDLASEFTGALPEPML
                     REFLTKFLPTATDKEAEAAMALEADGKLVEAKAAYEAILAADPNHGQALLGMARMAMN
                     DGDNAAALEYLERISIVADERKEADRLIARLNLQAGGVQNESELRAKIAADPKDIEAR
                     FELAQALAGVEKYDEALGEFLEVVKADRKFKDDGARKAMIQIFEVLDPDDPLTDKYRT
                     ELAAVLFR"
     gene            8078..>8413
                     /locus_tag="VMZ02_05155"
     CDS             8078..>8413
                     /locus_tag="VMZ02_05155"
                     /inference="COORDINATES: protein motif:HMM:NF037039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71351.1"
                     /translation="MALGTDTIESEVQRLGRKLYQQAAQNKPSLFESRDLLGRMIDWS
                     LDDESLRVALFRFVDVLPSLESSSEIGRHLEEYFTRVDHALSGLAILANVLHAGTVVA
                     PLVKRNVIKL"
BASE COUNT         1913 a         2247 c         2430 g         1823 t
ORIGIN      
        1 ccgatttttg ggagtataaa gcatgccgcg caccgcggtc gaggaattcg gtcggcgtgt
       61 acatctcggg acgagcatcg agtcacgaaa caatttcggc aaaccttccg gcggagctgc
      121 atcggagaag cgagcaactc aatataccgg cggcgatagg aaccgcgcag acgaagacca
      181 gcgggacttt ccggccaatt ctagcgcaag cgatgtacca agactttggc gcacggaggt
      241 gtggctaact cttcaacaga acagaaaaaa tcatccgtga ggcaaaactg ccacgagccc
      301 gcgtgacttg gcccgtagtt gttctgctac gcagtgtagt caaactgcga cacctggatc
      361 gggagtaaag gcgaagggta agcgccggaa tctaactagc gggtcctgcg cttcgccgta
      421 tacgatcgtt tttctacgtg atttgcagcg ccttgagcag cttcataaag tgagtgcggt
      481 gcatgcccaa cgctttagct gcatttgcac ggttgccttg gctgtttgcg agggcgcgga
      541 cgatgagttc ccggcggtaa gtgttgatgg catcgtggaa cgaagtttct tcggaacgac
      601 ccggcggctg ggcgccgatt acacgggcgg gcaaatggtg cagcgttaac tgcgggccat
      661 cgcctagaac gaccgcccgt tcaattacgt tggccaactc ccggacattg cctggccagc
      721 tgtaggccaa gagtttttcc tcggcgtcgc tggcgatggt tgaaaacttc ttcttggtgt
      781 cggcggcgca gcgttgtaaa aaataatttg ccagggcgga aatatcttct tggcgctcgc
      841 gcagcggcgg cagcgtcagg gggatgacat tcaatcgatg atacagatcg gcgcggaagg
      901 tagcggccgt aaccgcagtt tcgagatctc gattggtggc ggcgatgatg cgcagatcga
      961 cgctaatctt agcggtgccg ccgacccgtt cgaattcgcg ttcctgcaaa acacgcaaga
     1021 gcttggtttg cagctccaac gccagatcgc cgatttcatc aagaaacact gtgccgccat
     1081 gggcgaactc tagcttaccc ttcttcaatt gatgggcccc ggtgaacgcg cctttttcat
     1141 ggccgaatag atcgctttcg aggagttctt tggcgaggcc ggcgcagttg atggcgacga
     1201 acggcttcaa ccggcgttca ctccagtcgt gaatcgagcg agcgaagagc tccttgccag
     1261 tgccgctttc gccgaggagg agcaccgtcg atttgcttgt cgcagctttt ttggcttctt
     1321 cgacaatgcc gcgcatcgcg gcgctcgtgc ctgcgaccaa ccagttgcga ccgccgaact
     1381 cctgcgcgaa gaactcaacg tcctgtttga gttgggcgcg gtcaagggct tttttgatga
     1441 ctacggccaa atgttccggg tcgaatggtt tggtgatgaa gtcatcggcg cctgctttga
     1501 ttgcttcgac ggcgcgctcg atgctgccat gggcggtgat tataacgacc ggcaggtcct
     1561 tgtccgtctg gcgaatgttg cgcaagactt cgatgccatt catatccggc atctggtagt
     1621 cgagcagaac cagcgcggga ttgcctctgg cgattttttg tagtgcttgg gcgccgttgt
     1681 tcgcccgctc tgatgcgaag ccaagttcgt cgagctcttg ttcgagcagg tcgagattga
     1741 acggctcgtc atcgacaatg aggattttgc tcgccatgac gccgccctaa ttcatgagtc
     1801 ccaaaaaacg ccagtaaggt atggccaaca gcagcgcgac gatataggca atggcgtaaa
     1861 caggattgat ccgacacatg agcgggtacg aaaatccctt gccgtcggtg gtggagaagg
     1921 cgagcatatg ccagtcgact tgatatggga aaaaccaaac ttcggaggca atcagaatca
     1981 tcatggcgat gatccatgga ttcatcccga cgtcgatgga gagtggtagc agggcaacgc
     2041 acagtgtgac gacggctaaa aagcttgtaa agacgagttt caaaagatag gtcagcagcg
     2101 cgatcacgat aaaaaaccac gccgggctgt ccatgaaagg caggattaaa ggcgaaatca
     2161 catctaccag ccactgatcg atcttggctt gtttcatgag cgtcggcaga ctcagggtca
     2221 cgcccatatg aatcaacagc tcccagtcaa tacctttgcg catcataccc caatcgagaa
     2281 tcgaagtgtt gactagaacg gagaaggcaa tgatcgagat ccaggcgcca tcaatcccat
     2341 ggtacggtgc agtaagccag ccgcccacgg ttaacagcaa gacgatcaga ctaatccatt
     2401 ccttatgcga tagggggccc aaaatatcca gttgggtttg caccatctta taagagattt
     2461 gcgactgggt ttccgggcga tataagaaga tcgtcaccgc caaaataatg atgattacca
     2521 ccaacgtcgg cggtaacgcg gccaaaaacc aatagcccca gctgaattgc tcgcgcacgt
     2581 cggccggcaa taggccccag gcgatcaagc tggtagtcga accagtaaga aacaagaatc
     2641 ccagttgacc caggccgaga aagctcgcaa cgaaaagtcc ggtcgatgcc ttcgagggtg
     2701 ttttataacc gaggctgtcg gacagattga taagcatttg gctggtaatc actgtgcgcg
     2761 cggtctgttg tggaataagc gccatgacca ccacacccat gatgccggta gcgacgattt
     2821 gccagtgaaa gttcagagga aatagccgca cgagatgcag tgacaaacga tagaataggc
     2881 cgcagccggt gatggctgcg cctaacccta ggacaccaag tgtcataaac cacgtcgtgc
     2941 tggcgaagcc gcccatgacc gtcgacgctg tctcgagctt ggtgagaata acggccatgg
     3001 caaatactag agcaacaccg taatccggca acgtgcgaaa cacccaaaat atcagcgtta
     3061 aggaaatgag tcccagaagc tttgtgccag aaggctccaa gccgatgtcc ggtgtggcaa
     3121 accacaaata accgccgaca ctaaggccca acagcgcgcc aaaccattta ctcaagggcg
     3181 cggattgtaa cgctgctgca gaaaatcgcc agcgacgcgg cgcgtctgcg ctgctgagtt
     3241 ggcgattgaa cgacatagat accggctcgg gtgcgacatc gctgtagtgt tccggcaggg
     3301 tcatatcccg ttcgaccagc cccagagcgc tgcgcagttg gccgatcgct tggacgtatt
     3361 ctttgcgccg cgccagggtc tcggcgatgc ggcggtagct tgcgatcgct tctccagcgg
     3421 cggcccgctg ggccagtatt tcctggtagg cacgaaaagc gccgggtaaa tcgcccagat
     3481 ggatcaagat atcgcttttc aaattgacca gcgcggtgtc ggcaatgaaa tactcgcgtg
     3541 ggatgcgctc taaaatgttt ttgaccactg gcgccgcctc aaccagcgtt tccttactct
     3601 gggctttaac gagcttgatc acctcgggcc agtccggcac ttccgcggcg tggcgaacgg
     3661 cctgttgcca attgccgatg gcgacgtagc gcttagcgaa atcaccgtgt agacgttgct
     3721 gcgtgtcgct accatcgatg gtcagcaact tgtcgtgtaa aaaatctttg aagaaactgt
     3781 gaaactcgat ccggtcgttg ccgagacgtc gtatcaacgg agactgaccc ttgatcaagt
     3841 cggcgaccac gcgtcccacc gactttgact gaaacagctg gtcaatggtg gcgctgtcga
     3901 tggcatgcaa cagggatgca tggcgaaaga attcttgctg gcgcggcgat ctgccggcgt
     3961 agaactcttc ggcgagggaa ttaaaggctt cgcgcccgct ggaatactgg cgatcgacaa
     4021 aggataggcg cttactcaaa gtagcgcaga gttgcattag ccaggtggga tatctggcaa
     4081 ttaattcgtc ccagtcctgc cggctcaagc gccataggat cgaatctttt atcgtcacga
     4141 tggtcgccga acgcggctcg cccgagaaca aagccatctc cccgaagcaa tcttggggtc
     4201 ccaaaattgc cacgatttca gagttgccgg caccgctgtc gactaccact tgtacggccc
     4261 ccgactgaat caaataaaag gcatcgccgc gatcgccctg acgaactatt gtcgcgccgg
     4321 cggcgagatt gagctcttcc atcttgccca tgaccttggc gacatcctca cgggtcaagc
     4381 cggagaagat cgaaatgcca cgtatagtgt cggccagggg ttccatggct ggctcagcga
     4441 atgatcttgt cggcacgctt caagaccgcc ggcgacactt gcataccaat gaaggttgcc
     4501 gtgcgatagt tgatgataaa atcgaatttg tcggcgcgct caatcggcgg cagtgggttc
     4561 ttgggatttt tcaaaatgcg cccgatcata gccccggcgc gccggcccat gtcaagattg
     4621 ctcggaccgt acgccgcagt ggcaccctgg atcgcccagg attcgtcatt gaacatgctc
     4681 ggtaacttct tctgccgcgc cgtggtgaaa acgaactcag cttcgccctc caccaagtcg
     4741 tcgggaatat gaaaaagtgc agcgccgggc acggggcgca tactggtcaa agttgtcttc
     4801 aattcgtcag cagatttgac cccatgttcg accaccggca ggctgatttt ttttgctgcg
     4861 gcttgggcca acgcgacgtt gtcgcgggag aaactattat tgctatcgaa aagaatgtgt
     4921 agttcgcgta gctcgggaat cagttccttt agcaatgcca agcgcttctc cgttttctcc
     4981 acggcaaacc ccgccacccc agtgagattg cgctgggggt ccgtcatact cttcaccaag
     5041 ccggcggcta ccgggtcgcc gggatgaacg aacacgatgg gaatttcgcc ggtgacgcca
     5101 gccgctgccg tggtcgcgcg ggtgccggtg gtgaaaatca cgtccacctt gcgagcgacc
     5161 agatcgtccg cggcgcgagg cagtgcaccg cggtcgcctt tagcattgcg ggtttcgatg
     5221 acgaggtttt tgcgctcaat gtagcccaag ctcttgagta cgtcggtcaa ccctttgaga
     5281 gactgagact gtgggcggcc gagctctggg agtaaaacgc cgagacgcgc atgagtctgg
     5341 gtccggccct gggttaccgc gccagcactc atgaaaaaca acagcgtaat tgcttggagg
     5401 atgcttttcc ggagagatat cagcttgacc atcacagcag cttcagctcc aggccttgat
     5461 aggccacgat cgcgttaggg aacagagttt tgctgaaccg cgcaatgcgt ctcattgcgg
     5521 catcgtcatg ctgcggttca tggtgaaaaa gcacgagctg tttgacttgg gccttaacgg
     5581 cgacttctgc tgccatttcg atggtgctgt gaccccagcc cgctttcgac tttgttttag
     5641 aatagtactc gccgtgcaaa tactgcgcgt cgtgaatcag catgtcagcc ccccgggcaa
     5701 agtcgatgat gtcggcatag ccgcctgctt gctgttcaac gtcggttgca taaacgagag
     5761 ttttgccacg atattttatg cgatagagca tcaccccgtc tcgtggatgg gccgggcttt
     5821 tatgtgtgac gatcgacgca gcgccattcg cctgatttcg cgaaggtgga ctcctacgta
     5881 ccgcgagtcg ctcgccgggc gcggcaaatt ccagcgtctc gccgccgcca agtgagtgta
     5941 cttcgatgtt gcccctgagg tcgtcgaagg tgactgggaa aagattgttc tgggtcaact
     6001 gccgcaaagt ttgctgcaag gcgccgtttg agcgtcccgg cccgtaaata aacgaccggg
     6061 cgccggcggt cagcagcggt tcaaaaaaat acaggcccat caagtgatcg atgtgcgtat
     6121 ggctcaaaaa cacgtaactg gtgggacggc tggcggcacg ccggcaaatg tttttgccca
     6181 gtggaataat gccggtgccg gcatcgaaaa ttaagcgtcg gccggcgacg ctgatctcga
     6241 cgcaagaagt gtcgccgccg atttcgctgg tgctcgggcc ggtggtggga taactgccgc
     6301 gcacacccca aaagcgcacg cgaaaatcgc tgggtccaag tgtcatcgcc gccatgtaaa
     6361 gcagatttgc cggaaaaata aaagaatcat ccggagtgcg cggtggcccg acggtgattt
     6421 acaagtttac ggccccggcg gtttgacgta cgattcggct gcgaggcccc gcttcgctgt
     6481 gcacacccca agcttggttg gcgctgttgc tgctggttgg caaccgccgc aactcgcgag
     6541 taatgccggc agttacggat cgagcgtctc gttacgcctg tttggaacgc tggctcgcct
     6601 taatttcttg atccggctgt tggcgctacc aatcacgaac tgatctcacc cggacccatc
     6661 cgtttacccg gaagtgaggc ttgagttgat cgattggatt tctccagcca attctgaaaa
     6721 tataaatgag tgcgtaagta acggtcgcac ggaccaaaaa taagcgcgga ctgtacaaaa
     6781 ggagaacaca tgactgtcga taaagaattt ttcaacgtaa agtataaaga aggatcgctc
     6841 gacgcgaaaa ccgcgcagtt ggtgttcttc gccgtcagcg tagcgatcgg tcacgaggat
     6901 ggcgccaagc tacacttggc caaggcgcgc gagtgcggcg ccaccgagga tgagatcacc
     6961 gaggctgttg tttatgcgat gcgtcctgcg gcggcaaagg tgcgcaactt cgccaaagcg
     7021 gtaatcggca aatagtcgac cccagaccct gcgtcgttcg gaccgggcac actctctcgc
     7081 aaaggtggcg gataatatca tgagtcaatg gataatcgac gtgaacgagg aaaattttga
     7141 ggcggcggtg ttggagcgct ccagcgaagt gcctgtgctt gtggattttt gggctgagtg
     7201 gtgcgggccg tgcaaagcgc tggggccagt gctggaaaaa ctcgcggacg aatatgccgg
     7261 tggttttgtg ctcgcccgac tgaatgtcga tgaaaacccc aacttggcag gtatgtttgg
     7321 cattcaagga attcctgccg tgaaattatt tcgtgatggc gatttggcat cggagtttac
     7381 cggggccttg ccggaaccga tgttgcgcga atttctcacc aagtttttgc ctacggcaac
     7441 tgacaaagaa gcggaagctg ccatggcact cgaggccgac gggaaacttg ttgaagccaa
     7501 agctgcttat gaggcgattt tggccgctga ccccaatcat ggccaggcgc tgctcggcat
     7561 ggctcgaatg gctatgaacg atggcgataa cgccgcagcc ttggagtatc tcgagcgaat
     7621 ttcaatcgtc gccgacgaac gcaaggaggc cgaccgcttg atcgcccggt tgaatctcca
     7681 agccggcggt gtgcaaaacg agtcggaact acgggcaaaa attgccgccg accccaaaga
     7741 tattgaagcg cgcttcgaat tggcccaggc tctggccggc gtggaaaagt acgatgaggc
     7801 gctcggagaa tttctcgaag tggttaaagc cgaccgcaag ttcaaagacg acggcgcgcg
     7861 caaggcgatg atccaaatct tcgaagtttt ggacccggac gatcccctca ccgataaata
     7921 tcgcaccgag ttggctgcgg tgttgtttcg ctaaaattgt gacgccttag agtaccgctg
     7981 acacctgaag caccgacttc gcagcaattt tgtttaaaat gttcatgtcc tgcgtgtctg
     8041 ctgttaaacg atctcctctt taatcaatca gcatgcgatg gctctaggga ccgacacgat
     8101 cgagagcgaa gtgcagcggc ttggccgcaa actctatcaa caggctgcgc aaaataaacc
     8161 atctctgttc gagtcccggg atttgctggg ccggatgata gattggtcat tggatgatga
     8221 gtcgctgcgg gtggcgctgt tccgttttgt tgacgtcctg cctagtcttg aatcttcctc
     8281 tgaaatcggc cgccatctcg aagaatactt tacccgagtc gatcacgcgc ttagcggctt
     8341 ggcgattctg gcaaacgtgc tgcacgccgg cactgtcgtg gcgcctctcg tcaagcgtaa
     8401 cgtgatcaag ctg
//
LOCUS       DATLCH010000174         4116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6949953, whole genome shotgun sequence.
ACCESSION   DATLCH010000174 DATLCH010000000
VERSION     DATLCH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6949953"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VMZ02_05160"
     CDS             <1..229
                     /locus_tag="VMZ02_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71352.1"
                     /translation="MRRCGVWAMLRLFTYALLGKLQEIREFDSLSSKELWIDSRRKQL
                     AVALDGEVTRLRPPLHYRIHPASLTVMVPHG"
     gene            289..1146
                     /locus_tag="VMZ02_05165"
     CDS             289..1146
                     /locus_tag="VMZ02_05165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HUR71353.1"
                     /translation="MRTIVHLSDLHFGTVEREILAPLKQTILAAHPNLIAVSGDLTQR
                     ARRQQFVEARAFLDSLPFPKLVVPGNHDVPLYNFYARFADGLKRYRKYICADTEPFYQ
                     DDEIAVLGINTARSLTFKGGRISERQVELARERLAPLTGLVKIIVTHHPFDLPEHYSE
                     RALVGRARMAISAFAEFGIDLFLAGHHHVGFAQPSTWRFKINGHSALLIQAGTATSTR
                     RREELNSFNILGIDGATISVKRMVWSSQRGFVTQADEVFRGSDKGWTLQGQQDQASQI
                     STDKITPLS"
     gene            1448..1885
                     /locus_tag="VMZ02_05170"
     CDS             1448..1885
                     /locus_tag="VMZ02_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71354.1"
                     /translation="MRRFSYLASVVGLFIVGVPLGVHLLRSESVFTNHPSITAASSLG
                     KPETCLRLGFAARSGLYLSNVRVAIRDFHGDLVIDLVSDGPWLQVPLAPGTYHVAANF
                     DGTVHELRNVRLSDGDSVTHVLYWDLNIVPIELLAQIYGLNSV"
     gene            complement(1935..2690)
                     /locus_tag="VMZ02_05175"
     CDS             complement(1935..2690)
                     /locus_tag="VMZ02_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HUR71355.1"
                     /translation="MKSVGQDGQAGAIAPMTDFTAASYNIHQAVGADGRHDRRRIVQV
                     IKQLQADVIGLQEVDTNPDGDEMYQLDFFARETGLKAIAGRTMRRRDSDYGNVLLTRH
                     KILDVRTIDLSVPRREPRGAIDVDLDIGGVIVRVIVTHFGLSSRERFYQGDQLIKAVG
                     HRRDAMVIVLSDLNEWHPYGRTSRSIKPHFGRSTRLRTFPSWLPLLALDRIWVWPENA
                     LVSTRVHRQPPAQVASDHLPITAKITTAVCSQL"
     gene            2949..3692
                     /locus_tag="VMZ02_05180"
     CDS             2949..3692
                     /locus_tag="VMZ02_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HUR71356.1"
                     /translation="MNKKKSVRLAAVGDLHCTKNSQGKLQALFQQINQSGADVLLLCG
                     DLVDYGHLDEAIVLAKELAPIRIPMLGVFGNHEYESGQQEDVRRILADAGVTILDGDA
                     IEILGIGFVGVKGFGGGFDDHALQPWGEESTKQFVQQALTETLKLESALAKLRTEQRI
                     ALLHYSPIRATVVGEPPEIYPFLGSSRLEEPLNRYSVNAVFHGHAHRGAPEGRTQKNV
                     PVFNVAMKLLAQVYSDRPPFRIFEVPAPS"
     gene            3701..>4116
                     /locus_tag="VMZ02_05185"
     CDS             3701..>4116
                     /locus_tag="VMZ02_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71357.1"
                     /translation="MECLTDQTKTNNFYVSVLKTLNDANIPFLVGGACMLERITGIVR
                     PTKDFDLHVRPEQLPEIFAVLDRAGYQTEMTFSHWLGKVFQRTDYIDIIFSSGNGLCR
                     VDQEWFDHAIKGEIFGLPVLFCAPEEAIWSKAFIMER"
BASE COUNT          872 a         1119 c         1145 g          980 t
ORIGIN      
        1 catgcgccgc tgcggcgtgt gggcgatgct gcgtttgttc acttatgcgc tcctcggaaa
       61 gctgcaagag atccgcgagt tcgattccct gtcgagcaaa gaactgtgga tcgactcgcg
      121 gcgtaaacaa ctagccgtcg cgctcgacgg tgaagtgact cggctacgac cgccgcttca
      181 ttaccggatt catccggcct cgctcacggt catggtgcca cacgggtaaa aagatcgccg
      241 ccgagcccgc ttagctgcgg cgcacgttca cttcgttgtg ctcaccgtat gcgcacgatc
      301 gttcatttat ccgaccttca ttttggtacg gtggagcgag aaattttggc tccgctcaag
      361 cagaccatct tggctgccca tcctaacctg attgcggtat cgggcgacct gacgcagcga
      421 gcgcgccggc agcagttcgt cgaggcccgc gcatttctcg actcattgcc gttccccaag
      481 ctcgtcgtgc ccggtaatca cgacgtgccg ctgtataatt tttacgcgcg ctttgccgac
      541 gggttaaagc gttatcgaaa atacatttgt gcggacaccg agcctttcta ccaggatgac
      601 gaaattgccg tactcggaat caatacggca cgttccctga cgtttaaggg cggacgcatc
      661 agcgagcgcc aagtggagct tgcacgcgag cgtttagcgc cgctcaccgg gttggtgaaa
      721 ataattgtca cgcatcatcc gttcgatctg ccggagcatt attccgagcg ggctttggtg
      781 ggacgcgcgc gcatggctat ctcggcattt gccgaattcg gtatcgacct gtttctcgcc
      841 gggcatcatc acgtcggttt tgctcaaccc agcacttggc gcttcaagat caacggccat
      901 tcggcacttc tgatacaagc cggcacggca acatcgaccc gtcgccgcga ggaattaaat
      961 tcgttcaata tcctcggcat cgatggcgca acgatttcag tgaagcgcat ggtttggtct
     1021 tctcaaaggg gttttgtcac gcaagctgac gaagtttttc gtggtagcga taagggctgg
     1081 actcttcagg gacagcagga tcaagcgtcg caaattagca ccgataaaat aacgcctctg
     1141 tcctaattac ggtacatttt cggcgagtct gcgggggaaa ttagattggc atgcgatttg
     1201 gactgtatgg ctcaaagaag cttcaggagg ttcaaaatgg gtcaatcggt ccaagagtgc
     1261 ggcgggcaca agcaagatcc tcgaagactc ttagggttga tatgctcatc ctgcggtcgc
     1321 ggctttacgt ccgaagaaga gctccgggac caccaagtgg cttgtcgcag cagctttgaa
     1381 ttcctcagcc agtttgaatc ccttgcgccg aattacgact tgttaaattg atcagtcaat
     1441 taaagcgatg cggagattct cctacctcgc ttcagtagtc ggtctcttca tcgtcggtgt
     1501 gccgctgggc gttcacctgt tgcgcagcga gtcggttttt accaaccatc cttcgatcac
     1561 cgccgccagc tcgttgggca aaccagagac gtgcctgcgg ttgggcttcg ccgctcgctc
     1621 cggtctttac ctttccaacg tgcgtgtggc gatcagagat ttccacggcg atctggtgat
     1681 cgacctcgtt tcggacgggc cctggctgca agttccgttg gctccgggga cttaccatgt
     1741 cgcggcgaat tttgacggca ccgttcatga gctgcgcaac gtgcgcctca gcgacggcga
     1801 tagtgtcact catgtcctct actgggactt gaacatcgtg ccgattgaac tcctcgcaca
     1861 gatatacggc ttgaacagtg tttaaccgtt gcttcgagca tgctgaccgc ttcatggcct
     1921 gcaggcgagc gggctcagag ttgcgaacac accgcagtgg taatttttgc cgtgatcggt
     1981 aaatgatccg aagcaacctg cgccggcggt tggcgatgca cgcgcgtcga aactagggcg
     2041 ttttcaggcc atacccaaat acgatcgagg gctaacagcg gcaaccagga aggaaatgtc
     2101 cgcagccgtg tggatctgcc gaagtgcggc ttgatgcttc gcgaggttcg gccataggga
     2161 tgccactcgt tgaggtcgct caaaactatc accatggcat cccggcggtg accaaccgct
     2221 ttgattaact ggtcaccctg gtagaagcgt tcgcggctgc ttaagccaaa atgggtcacg
     2281 atgacgcgga cgatgacacc gccgatatcc aagtcgacgt cgatggcgcc gcgcggttca
     2341 cggcgcggaa cgcttaagtc aatggtgcgg acatcgagaa ttttatggcg agtcagcagt
     2401 acattgccat aatcgctatc gcgtcgtcgc atggtgcgac cggcaattgc ctttaagcca
     2461 gtctcgcgcg cgaaaaaatc gagctgatac atttcatcgc cgtcaggatt ggtatcgact
     2521 tcctgtaatc cgataacgtc ggcttgcaac tgctttataa cttggacgat gcgccggcga
     2581 tcgtgccgtc cgtccgcgcc gaccgcctga tgaatgttgt aagaggcggc ggtgaagtcg
     2641 gtcatcggcg ctatggcacc ggcttgacca tcttgaccaa ctgatttcat aacgggaatt
     2701 gcttttgaat ttttgccact tctccaaccc gggttcaaca cggcgttaaa acttaggtga
     2761 tatatcatct cctgtgcggc cgtcctagaa ctgcgacgcg gacatcaatt tgtccgactg
     2821 ctaggacgag gagagtactt ttagaattgc gaaagtattg cgaatccgag gatgcatggc
     2881 atgtcagttg ctcgctcgga ttcggagtat gtattttctt cgacacagtc ccgagtgcgt
     2941 agcacgacat gaataagaaa aaatcggtcc gattggcggc cgtcggcgac ttacattgca
     3001 ccaaaaactc ccagggcaaa ttgcaagcgc tgttccagca aattaaccag agtggtgcgg
     3061 acgttttgct gttgtgcggt gaccttgtcg attatggaca cctggatgaa gcgatcgtgt
     3121 tagctaagga attggcgccg attaggattc ctatgctcgg ggttttcggc aatcacgaat
     3181 acgagtcggg ccagcaggaa gacgttcggc gtattttggc ggatgccggc gtgacgattc
     3241 tcgatggcga tgccatcgaa attcttggca tcggctttgt tggcgtcaaa gggtttggcg
     3301 gtggcttcga cgatcatgcg ctgcaaccct ggggcgaaga atcgaccaag caattcgttc
     3361 aacaggcgct cacagagact ctcaaactgg agtccgctct ggctaagctg cgcaccgaac
     3421 agcgtatcgc actactccat tactcgccga tccgtgcgac ggttgtcggc gagccgccgg
     3481 agatctatcc atttcttggg tcaagccgac tcgaagagcc gcttaaccgc tattcggtta
     3541 atgcggtttt tcatggccac gcgcatcgcg gtgcgcccga ggggcggacc cagaaaaacg
     3601 tgccggtgtt caacgtggcg atgaagttac tcgcacaagt ttactcagat cggccgcctt
     3661 tccggatttt tgaagttcca gccccctcat gagcagcctt atggaatgct tgacggatca
     3721 aactaaaacc aacaattttt acgtcagcgt gttaaaaacg ctcaacgatg cgaacattcc
     3781 gtttctcgtc ggcggcgcct gtatgctcga gcgcatcacc ggcatcgtcc ggccgaccaa
     3841 agactttgac ctgcatgtgc ggcccgaaca gctgccggaa atatttgccg tcctcgatcg
     3901 cgcgggttac cagactgaga tgactttctc ccattggctc ggcaaggtat tccagcgcac
     3961 cgattacatc gacatcatct tcagctccgg aaacggccta tgccgcgtgg accaggagtg
     4021 gttcgatcac gctatcaagg gtgagatttt cggtcttccg gtgctttttt gcgctcccga
     4081 agaggcgatc tggtccaagg cttttatcat ggaacg
//
LOCUS       DATLCH010000175         8337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8439256, whole genome shotgun sequence.
ACCESSION   DATLCH010000175 DATLCH010000000
VERSION     DATLCH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8439256"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..1196
                     /locus_tag="VMZ02_05190"
     CDS             459..1196
                     /locus_tag="VMZ02_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HUR71358.1"
                     /translation="MITQSVRDELAPSGKLRIGINYGNAVLASRDAVSGKLSGVAVDL
                     AQELGRRCELAVETVAFDSAGKMTAALQTHTWDAAFLAVEAGRANEIAFTAPYLLIEG
                     TYLLPPGSAIHSIADVDRAGVRVGVSAGSAYHLYLSRNLHHAQMVEAENPQTAFDLLI
                     DGKVDVVAGVRQALTANAAKLNGARVFDERFMAIEQAMGMPHGRPAGLQFLCEFIEEV
                     KACGFVAWALERAGIRGVAVAPPIERN"
     gene            1368..2693
                     /locus_tag="VMZ02_05195"
     CDS             1368..2693
                     /locus_tag="VMZ02_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71359.1"
                     /translation="MIQKIMEGNNRSTPRRPFALPFYYGWFIIGLSFFAYLAASAVRS
                     APSVLIHPLEREFGWGRTAISSAASLNLLTYGLMAPIGGWLLDRYGARRVVVGSLATI
                     AIGVTGTVFVTELWHFILLWGVVLGIATGVTPALSASVASRWFIARRGLALGILTNAN
                     ATGQVIFLPILMALIVSRGWRPAMMLIVATALLLLPAVWVWLRDYPSEVGLEPFTDST
                     TTVEQRNQARAVPRAFSFAALGDAIKSPTFWLLSGSFFVCGMTSNGLVGTHMIPHAIE
                     RGIPEVAAATAVGIMGVASFIGTMFAGWLCDRIDARKVLSAAYLFRGLSLFVLPYVTD
                     SSSLFVFAILYGFDWFATGPSTVALIAKHFGQDRVGVLFGLVFVSHQIGSAIAALGAG
                     WIYSEYGAYYYAFVSGAVMALLAAGMAMMIRRDRDNAPAQAILVEVARA"
     gene            complement(2906..3406)
                     /locus_tag="VMZ02_05200"
     CDS             complement(2906..3406)
                     /locus_tag="VMZ02_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71360.1"
                     /translation="MGFTLESFSADVQRILQADPGRPGREKVRDLVQEALKDEKFIST
                     YINDDTADRQVIYEDPELGFCVCAHVNREAREANPHDHGSSWAIYGQAFGETEMSDWE
                     VIEAAAEGTPGKVKRGRVYTLKPGMAHIYNEGDVHSPKRVAATGLLRIEGKNTTKLKR
                     YSYKAV"
     gene            4136..5092
                     /locus_tag="VMZ02_05205"
     CDS             4136..5092
                     /locus_tag="VMZ02_05205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUR71361.1"
                     /translation="MDYKILGRTGLTVSVAGLGCGGPSRLGLSGLKGQTHSESEVVAH
                     VRCAIDLGINFFDTAEWYGTEVVVGKAIAGVPRDRLVISTKKNIVPEDHGEPEKEIRR
                     SLEQSLKNLGTDYVDVYHVHGAEPKDYPNAVERHLPTLLRLREEGKIRAVGITERFVV
                     DSSHQMTERAVRDNFWDVIMVGFNLLNPCARRNIFPLTSANRVGVLVSYALRRALSQP
                     QRLKKLCAELVSKGAIPAGALNLDEPLDFVTTDGAAHSIQDAAYRFCRHEPGIDVVLT
                     GTGNPEHLKANVQSLCRPRLPESVQLRLKELFGKIDGLTGNQ"
     gene            5119..6144
                     /locus_tag="VMZ02_05210"
     CDS             5119..6144
                     /locus_tag="VMZ02_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71362.1"
                     /translation="MGERIIRNFMIVILAVLLAYGQAAAQESFFKGKTVRIVVGFAAG
                     GGFDTYSRAMARYWGRHIPGNPSIMVENMAGAGSLIAANHVYNAARPDGLTIGNFGGG
                     LFMQQLKGGAGIEFDARKFEFLGVPVQDNRSCAFTKASGITSMERWMAAKSPVKIGAT
                     APGDLVHDGPKILQAALGLPIHLVSGYKGTADIRLAAEAGELAGGCWGWDSIKATWAR
                     ALESGDAIVVVQMVSKPHPDLPKVPLAISYAKSEDARQLIEAGVTIPAITNRPYVVRP
                     GTPKDRVEMLRKAFDATMKDPEFVADAKKSRIDLEPIGGLEVQKMVGDMFNLSPALIA
                     KLRDALK"
     gene            6264..7295
                     /locus_tag="VMZ02_05215"
     CDS             6264..7295
                     /locus_tag="VMZ02_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71363.1"
                     /translation="MIDKIRAGFLGLAAASALTLAVTAQAQDIYKDKTLTFIVGYSPG
                     GTYDQYTRLLARHIGKYLPGNPTRIVENMPGAGGIIAANHLYNRAKPDGLTIAAWASP
                     LILQHVMGNDAIKIDGRKVGWIGIPGPYDTACHFGSASGIRTMDDWINAKRPMKIASI
                     GPGTSLSDVPKLLKAALNLPLEMVEGYKGGAEARIAVESGEVDGLCASWQATKVTWRS
                     QMDTGKIHVVLQATFKSHPDLKKVPLAINYAKTEEAKILLRVADNVHVYQFPFSVAPG
                     TPADRLAALQQAFVKTFRDRELVAEADKAQLEVAPIDGPTTTKTFAGLYELNPALVAK
                     LKDILIPKK"
     gene            7341..8210
                     /locus_tag="VMZ02_05220"
     CDS             7341..8210
                     /locus_tag="VMZ02_05220"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71364.1"
                     /translation="MRKVVVTGGSGKLGQFVIRDLLERDYSVLNLDRIVPREKLCMTW
                     QVDLRHSGDLFEALRGAYGIIHLGAYQAPNLAPDAETLSNNVSATYNVLRAAADMDVK
                     RVVLASSTAAFGFIYASRLWAPKYLPLDEAHPSTPQDSYGLSKVLGEQIADSIVSANW
                     KMTVSSLRFPGVNFDLSYESFRDRWRNPSTRASGFWTYIDARDAAMTCRLALEAKFTG
                     HQVFIASAAKNCMIQPSRELVQKYLPKGTRFKKAAGTHWSCVDSAKARRMLGFKPQHL
                     WQNYLPEGQEQKY"
BASE COUNT         1756 a         2151 c         2503 g         1927 t
ORIGIN      
        1 ggccgtgacc tgatgggcac cgacgttgag ttcgctggcg atcaattgag taatcttgtt
       61 ttggattgcg tgagaaatgt tttcggtcat gatgaaacct tctaaattga gtgaatcgag
      121 aaattatagc aacgttgggc tgagagtgaa gtgtcatgaa agaaaagacg cacgattaat
      181 cgagaaatga tccgaccggc tttagctcga ggagtttttt ggtccacttc aaaccgattt
      241 tctgaccggt gcaagaagga ctttatcttc gtagtagaga cttgaagccg atcgggatgg
      301 atgccggcgc tcgccggcat tacgattcat ttttttctgg aggtacgtgt cgaaagccat
      361 tcgtcggaag agttcagaaa ttgacaatgg agaatcgatc gctggatggc gtgcttgggg
      421 tgattgaccg ttgcggttgg agcgcgtagc ttcgcggaat gataacacag tcggtacgag
      481 atgaactggc gccatcgggc aaactgcgaa tcggtattaa ctatggcaat gccgtgttgg
      541 cgagcagaga tgcggtcagc ggcaagttga gcggcgtcgc ggtggatctg gcgcaggaac
      601 ttgggcgccg atgtgagctg gcggtggaaa ctgtcgcttt tgactcggct gggaaaatga
      661 ccgcggcgtt gcaaacgcat acttgggacg cggcgtttct cgcggtggaa gcgggccggg
      721 ccaacgagat cgcgttcact gcgccttatc tgttgatcga aggcacctac ttgctcccgc
      781 cgggatccgc gattcattcc attgccgatg tcgaccgcgc cggcgtccgt gtcggagttt
      841 cggcgggtag tgcctaccat ttgtatttga gccgtaactt gcatcacgcg caaatggtcg
      901 aggcagagaa tccccagaca gcatttgatc tgctgatcga cggtaaagtg gatgtcgttg
      961 ccggcgtcag gcaggcgctg accgctaacg ctgctaagtt aaacggagcg cgcgtgttcg
     1021 acgagcgctt catggcaatc gaacaagcga tgggtatgcc gcatgggcgt ccagccggct
     1081 tacaattctt atgcgaattt attgaagagg taaaagcctg cggctttgtc gcctgggcgc
     1141 tggagcgcgc tggcattcgc ggcgtcgccg tggcaccgcc gatagagagg aattagccgc
     1201 aaaagacgca aagtgcgcaa gaatatttct tcaccggaga gacgccgagt tgccagagtt
     1261 cggattgtca aaaataatct ttgtctgtgt gctcagcgcc tctgcggtga actctgtttt
     1321 cttattcgcc gtggtttggc gctcgtacct atacgctcgc ataggttgtg atacagaaaa
     1381 ttatggaggg caataaccgt tctacgccgc ggcggccgtt cgcgctgccg ttttactatg
     1441 gctggttcat aatcggtctt agcttttttg cttaccttgc cgcgtcggcg gtgcgctcgg
     1501 cgccttcggt gttgattcat ccattggaac gcgagttcgg ctgggggcgc acggcgattt
     1561 cttcggcggc gagtttaaat ttacttacct acggcttaat ggcgccgata ggtggttggc
     1621 tgctcgatcg ctatggcgct cggcgcgttg tggtgggcag tctcgcgacg attgccatcg
     1681 gtgtgaccgg cacggtgttc gtcacggaat tgtggcactt tatccttctt tggggagttg
     1741 tcctcggcat tgccaccgga gtgacaccgg cattgtcggc gagcgtggcg agccgctggt
     1801 tcatcgccag gcgcggtttg gcgctaggga ttctcaccaa cgccaatgcc actgggcagg
     1861 taatcttttt accgattctg atggcgttga ttgtttcacg cggttggcgc ccagcaatga
     1921 tgttgatcgt agcgacagcg ttgctattgc taccagcggt ctgggtgtgg ttgcgcgact
     1981 atccctcgga agtcggactc gaacctttta ccgatagcac gacaaccgtg gaacagcgca
     2041 accaggcgcg cgcggtgccg cgcgcgtttt cgttcgccgc tcttggcgat gcgatcaaga
     2101 gtccaacgtt ttggctgctc agcggcagtt tttttgtctg cggcatgacg tccaacggcc
     2161 tggtcgggac tcacatgatt ccgcacgcca tcgagagggg cattcctgaa gtggcggcag
     2221 ctacggcagt cggcattatg ggtgtggcga gtttcatcgg tacgatgttt gccggttggt
     2281 tatgcgatcg catcgatgcg cgtaaagtat tgtcggcggc gtatctgttc cgcggtttgt
     2341 cgctgtttgt tttgccgtac gtgaccgatt cgtcgagtct gtttgtcttt gcaattttgt
     2401 acggcttcga ttggttcgcc accggaccct cgacggtggc gctcattgcc aaacactttg
     2461 gccaggaccg cgtcggcgtg ctattcggtt tggttttcgt atcgcatcaa atcggcagtg
     2521 cgatcgcggc acttggcgcc gggtggatct acagcgaata tggcgcatac tactacgcgt
     2581 tcgtgagcgg agccgtaatg gcgctcctgg cagccggtat ggccatgatg attcgccggg
     2641 atcgcgacaa tgcgccggcg caggcaatct tggttgaagt tgcgcgcgcg tgaattggct
     2701 cgcgcgccga gccctttcag attccaaatt ccagattcac tagaccgatc agatcagttg
     2761 ccgaagtgtg tgccgctgtc gctgatcaat cgagatgcgc tgcaagaatc aaataaacgg
     2821 taaaaaaatc catcggcggc gtagtaatga gttcaactac gccggcacga tggcgaaaac
     2881 acgcgggaga agagaatttg tcggtttaga cggctttgta cgagtaacgt ttcagtttcg
     2941 tcgtgttctt gccctcgatg cgcaataaac cagttgccgc gacacgcttg ggcgagtgga
     3001 catcgccttc gttgtagata tgcgccatgc ccggcttgag cgtatagacg cgaccgcgtt
     3061 tgacctttcc cggtgtgcct tcggcggcgg cttcgatgac ttcccagtcg ctcatttcgg
     3121 tctcgccaaa ggcttggccg tagatcgccc aagagctgcc gtgatcgtgc ggattggctt
     3181 cgcgcgcctc gcggttgacg tgagcgcaca cacagaagcc cagttccgga tcttcgtaga
     3241 tcacttggcg gtcggctgtg tcgtcgttga tgtaggtcga gatgaacttc tcgtccttga
     3301 gagcctcctg caccaagtcg cgaacttttt cgcggccggg gcgacccggg tcagcttgaa
     3361 gaatgcgctg cacatccgct gaaaacgatt ctagcgtgaa acccatgagt tcatcctcct
     3421 gttatgttag tcatcaaatc attaacaaag tgccgtggct accgtcaatc gccaagcgtt
     3481 ggcacggtaa gtgacatccg gatcgtgacg ggcaaagata tttcgacccg ctgaggctgg
     3541 attgactgta accgaatgca gcactataag cgcaactatg cggcaatcgc atctcattgg
     3601 acgcagtttt ggcagagtag cttttgcact tgcgatgagc ttgttgcctg caagctgttc
     3661 cgccgcgcct acgcgcgatc cttatgacac ccaacccatt ccggaaggcg ctaagtttga
     3721 gggaaaatgc acttttctcg gggagatcgg ttgtagtttg tcctcgctgt tctccggcga
     3781 tgcgggctat gagcgaagag ccgcatgcat cgcctttgtc gagccgaacg ggacgaaagt
     3841 cgaacaatgc ggttcggtat ccggcactaa gcgccttccg taaattgaca cctgtttacc
     3901 ctctaacgtt gttggtaatg atgaaccgaa ccctcacctg gtattatggt tgctgaaaat
     3961 tgcacttcat tgggaggtgc tgtgaaagct aaccaatacg attatatctt tgctgttcaa
     4021 ttttgcgcgt aagcggcgga gggattgcgg cactggcaaa catcgattct cagatcggct
     4081 tcggtatatc tcgcgcaatg gcgtaaagac gccagcttcc aaaaagaaaa gatacatgga
     4141 ctacaaaatt ctcggccgca ctggattgac ggtcagcgtc gctggattgg gctgcggcgg
     4201 gcccagccgg ctggggctgt cgggattgaa gggacagacg catagtgaga gcgaggtggt
     4261 cgcgcatgtg cgctgcgcca tcgatctagg catcaacttt ttcgacacgg cggagtggta
     4321 cggcactgaa gttgtcgtcg gtaaagcgat cgcaggcgtg ccgcgcgatc gcttggtgat
     4381 ttccaccaag aaaaacatcg tgccggaaga tcatggcgaa ccagaaaaag aaattcgccg
     4441 ttctctcgaa cagagtctta aaaatctcgg caccgactat gtcgatgtct atcatgtcca
     4501 tggcgccgaa ccgaaagatt accccaacgc cgtcgaacgc catttgccga cgctgctgcg
     4561 cctgcgcgag gaggggaaaa ttcgcgccgt ggggattacc gaacgtttcg ttgtcgactc
     4621 gtcgcatcaa atgaccgaac gcgctgtgcg cgataatttc tgggatgtca tcatggtcgg
     4681 cttcaatctg ctcaatccct gcgcgcggag aaatattttt ccattgactt cggccaatcg
     4741 cgtaggagtt ctcgttagtt atgcgcttcg ccgggcgttg agccaaccgc agcgattgaa
     4801 aaaactttgc gccgagcttg tcagcaaagg cgcgattcct gctggcgcgc tgaacttaga
     4861 cgagccgttg gattttgtta cgacagacgg tgctgcgcat tcaattcaag atgcggccta
     4921 ccggttttgc cgccatgagc cgggcatcga cgttgtgctc accggcacgg gtaacccgga
     4981 acatctcaag gccaatgttc aatcgctgtg ccggccgcgg ttgccggaat cagtgcagct
     5041 gcgcttgaaa gaactgtttg gcaaaatcga tggcttgacc ggcaatcagt aatacaggtt
     5101 acaaaagaga ggaagtctat gggcgagcga ataattcgta atttcatgat tgtgatactg
     5161 gccgttcttt tggcgtacgg gcaagcggcg gcgcaggagt catttttcaa aggaaaaacc
     5221 gtacgcattg tcgtcggctt tgctgccggc ggcggcttcg atacttactc ccgcgcgatg
     5281 gcgcgctatt ggggccggca tattcccggc aatccgtcga tcatggtcga aaacatggcc
     5341 ggtgccggca gcttgatcgc cgccaatcat gtttacaacg cggcgcggcc ggatggcttg
     5401 accatcggca attttggcgg cggtctgttt atgcaacaac tcaagggcgg cgccggtatc
     5461 gagttcgacg cgcgcaagtt cgagttcttg ggcgtccccg tccaagacaa tcgctcctgc
     5521 gcgtttacca aggcgagcgg catcaccagc atggaacgat ggatggcggc gaagagccca
     5581 gtaaagatcg gggcgacggc gccgggggat ttggtgcatg acggaccgaa aattttgcag
     5641 gcagcgctcg gtctgccgat tcacttggtt tcagggtaca agggcacagc agacattcgg
     5701 cttgcggcag aagcaggaga gctggcgggt ggttgctggg gttgggactc gatcaaagcc
     5761 acgtgggcgc gagccctcga aagcggcgac gcgatcgtgg tggtacagat ggtctcaaag
     5821 ccgcatcccg atctgccgaa ggtgccgctg gcgatcagct atgcaaagtc cgaggacgcg
     5881 cgccagctga tcgaagcggg ggtgacgatt ccggctatta ccaatcggcc ttatgtcgtt
     5941 agaccgggca ctccaaaaga tcgagtggag atgctgcgca aagccttcga cgcgacgatg
     6001 aaggatcccg agtttgtcgc cgacgccaaa aaatcgcgca tcgatctcga acccatcggc
     6061 ggcctggaag tacagaaaat ggtcggcgac atgttcaact tatcgccggc gttgattgcc
     6121 aagctgcgtg acgcgctgaa gtgatggcgc cgggagtgac gctcattaca aattcaaaat
     6181 tccagattcc aaatggtgac cgcaatggaa cggcgctatg ggttgtagta gtttgggcga
     6241 cgacgtgatt cacgggaggt ccgatgattg acaaaattcg agcgggtttt cttggtcttg
     6301 cggcagcgag cgcgctaact cttgcggtaa ccgcgcaggc gcaagatata tataaggata
     6361 agacgctgac cttcatcgtc ggttattcgc cgggcggcac ctacgaccaa tacacgcggc
     6421 tgttggcgcg gcacatcggc aagtatttgc ctggcaatcc aactcggatc gtcgagaaca
     6481 tgcccggtgc cggcggcatc attgcagcaa atcatcttta caaccgggca aagccggatg
     6541 gactgacgat tgccgcgtgg gcgtcgccgc tgatcttgca gcatgtgatg ggtaatgacg
     6601 cgatcaagat cgacgggcgc aaggttggct ggatcggcat acccggccct tacgatacgg
     6661 cgtgccattt cggcagcgcc agcggtattc gcacgatgga cgattggatc aatgctaaac
     6721 ggccgatgaa aattgcttcc atcggtccag gaacgagtct gtccgatgtc ccaaagcttt
     6781 tgaaagccgc gctgaatcta cctctggaaa tggtcgaagg ctacaaaggc ggcgccgaag
     6841 cgcgcatcgc cgtcgagagc ggcgaagtcg atggtctatg tgcttcgtgg caagcgacta
     6901 aggtgacgtg gcgcagccag atggacacgg gcaagattca tgtcgtcctg caagcgacct
     6961 ttaaatccca cccagatctt aagaaggtgc cgctggcaat caactacgcc aagaccgaag
     7021 aagcgaaaat tctcctgcgg gttgccgata atgtgcatgt ctaccaattc ccattttcgg
     7081 tcgcgccggg aacgcctgcc gatcgactag cagccctgca acaggcgttc gtcaaaacct
     7141 ttcgcgaccg cgaattggtc gccgaagcgg acaaggcgca attggaagta gcgccgatcg
     7201 acgggccgac cacaaccaaa acgtttgccg gcttgtatga attgaacccg gcgctcgtcg
     7261 ccaagttgaa ggacattctg attcccaaga aataagccgc ccactgcaaa atcggttcag
     7321 tattaacggt aaattactct atgagaaaag ttgtcgtgac gggtggcagt ggcaagttgg
     7381 gtcagtttgt cattcgcgat ttactcgagc gggattatag cgtgctcaac ctcgatcgca
     7441 tcgtgccgcg ggaaaaactc tgcatgacct ggcaggtcga tctgcgccat tctggagatt
     7501 tgttcgaggc gctgcgcggc gcctacggga taattcatct gggcgcttat caggcgccca
     7561 atctggcgcc ggacgccgaa actttgagca acaacgtttc ggcgacctac aacgttttgc
     7621 gcgcggcggc cgacatggac gtgaagcgcg tggttttggc gtcgagcacg gcggcgttcg
     7681 gcttcattta cgcgagcagg ctttgggcgc cgaaatattt gcctttggat gaagcgcacc
     7741 catcgacacc gcaggattcc tatggactat ccaaggtgct gggcgagcag atcgcagact
     7801 caatcgtttc ggcgaattgg aagatgacgg tgagcagcct acgtttcccc ggtgtgaact
     7861 ttgacttgtc ctacgagagt tttcgcgacc gttggcgcaa tccttcaacg cgggccagcg
     7921 ggttttggac ctacatcgac gcccgcgacg cggcgatgac ctgccggctg gcgctcgaag
     7981 cgaaattcac cggccaccaa gtgttcatcg cctcggcggc gaagaattgc atgatccaac
     8041 cgagccgcga gctggtgcaa aaatatctgc ccaagggcac gcggttcaaa aaagccgccg
     8101 gcacgcattg gagctgcgtc gactcggcca aggcgcggcg catgctcggg ttcaagccgc
     8161 agcacctctg gcagaattat ttgcccgaag ggcaggagca gaagtattga aacgtttagt
     8221 ttgctgtctg attttcagcc tgtcgacgtt cagctcgaaa cctcaaattg ttccttttta
     8281 tgcgtgccgt gcgtaacgat ctgagttcgc ccgactccgt cgttgctgca cgtgctt
//
LOCUS       DATLCH010000176         3812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3234855, whole genome shotgun sequence.
ACCESSION   DATLCH010000176 DATLCH010000000
VERSION     DATLCH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3234855"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VMZ02_05225"
     CDS             <1..470
                     /locus_tag="VMZ02_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71365.1"
                     /translation="LNAYFAGRCDVYTTDVSGLIAARASRAAVAGNHVILPEIISKEP
                     FGPAVRHGDDHWFDIVRWTLFAMIEAEELGITSKNVDQQAQNANPVMQRFLGAKGDFG
                     KMLKLDNRWAFNVIKQVGNYGETFERHLTPLGVDRGINNLWNKGGLMYAPPLR"
     gene            509..1495
                     /locus_tag="VMZ02_05230"
     CDS             509..1495
                     /locus_tag="VMZ02_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HUR71366.1"
                     /translation="MARRLDPRQRAGLCQILLLVALCMAGWYFLTNAVDNLLSRRIAS
                     GFGFLWREAGFEIGESTLLSASAADSYLKALTVGLLNTFRVALLASTFATLIGGTLGL
                     ARLSPNWLLAKIAAGYVELLRNIPLVVQLFFWYAVITESLPAPAEALNPVPGFYLSNR
                     GLAVPTIVTLLPLQIEHPTLNAFNFSGGVVLSPEFTALFLGLALYTAAFIGEIVRAGI
                     LAVDRGQYEAAQAQGLSQGQIMRLIILPQALRLIVPPLTSQYLNCIKNSSLAVAIGYP
                     DLVSIANTVINQTGQAIEGVAIIMLVYLTISLTISAAMNFYNRRIALRGART"
     gene            1492..2598
                     /locus_tag="VMZ02_05235"
     CDS             1492..2598
                     /locus_tag="VMZ02_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HUR71367.1"
                     /translation="MITRPRVTIAHPAALLHWTRIRLFATWRSSCVTLAIVYLAAKLL
                     PPLFDWALLRAVWSPTHAALCRDTRIAGACWAFVADNYRFILFGTFPAEEQWRPALVI
                     VSLLALYALSGFHLLRRPTLLRLWFGELLVIAMLMWGGVFGLTYVENERWGGLTLTLL
                     LSTFGIALAFPLAITLALGRRSGMPLIRWLSIGYIELIRGVPLISVLFMASVMLPLFL
                     PSGVSIDKLLRAQVAMILFAAAYLAEVIRGGLQAIPAQQEEAALATGLGYWDRVRYVM
                     LPQALRIAIPPLVNTFIGFFKDTSLVVIIGLFDFLTSIKVSLTEPTWSGFGVEAYLFA
                     ALGYFAFCYPMSRYSQRLERQTGYGTSLRALRDN"
     gene            2649..3395
                     /locus_tag="VMZ02_05240"
     CDS             2649..3395
                     /locus_tag="VMZ02_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HUR71368.1"
                     /translation="MRKLLVCQHVPHEILGTLNPMLKRAGFRIRYVNFGRHPDAEPTL
                     DGYDGLVVLGGPMSAYDDPRLPHLTVEMKLIESAMQRNLPVLGICLGAQLIARTLGAR
                     VYANHIKEIGWYSVAPSDQAEQDPLFSGFGKSETLFQWHGDTFDIANGAIHLAWSRHC
                     TNQAFRYGNNVYAFQFHLEVDEPMILRWLKVPENRKEIASLHGDIDPDQIHLQTGRHI
                     DRLHELSDRVFGEFIELFGLDKDRRRLVSR"
BASE COUNT          759 a         1129 c         1034 g          890 t
ORIGIN      
        1 tgctgaacgc ctacttcgcc gggcgctgcg acgtctacac caccgacgtc tcaggtctga
       61 tagctgcgcg cgcttcccgg gcagctgtcg cgggcaacca tgtcatctta ccggagatta
      121 tttccaaaga gcctttcggc ccggccgtgc gccacggcga cgatcattgg ttcgacattg
      181 tccgttggac tctattcgca atgatcgaag ccgaagagtt aggaatcacc tcgaagaatg
      241 tcgaccagca ggcgcaaaac gccaacccgg tgatgcaaag attcctcggc gccaaaggcg
      301 atttcggcaa aatgctcaag ctcgacaacc gctgggcttt caatgtcatc aaacaggtcg
      361 gcaactacgg cgaaaccttc gagcgccatc taaccccatt gggcgtcgat cgcggcatca
      421 acaacttatg gaataaaggc gggctcatgt acgcgccgcc actgcgctga gcgcagcgaa
      481 ctcgactcat caaaagcggc aaatcccgat ggctcggcga ctcgaccccc gtcagcgtgc
      541 cggactttgt cagattttgc tgctggtcgc cctgtgcatg gcgggttggt atttcctcac
      601 caacgccgtt gacaacttgc tgtcgcgccg tatcgccagc ggattcggct ttctctggcg
      661 cgaggcgggc ttcgagattg gtgagagcac gctactatcg gcgagcgccg ccgacagtta
      721 tttgaaagcc ctgaccgttg gtctactcaa cactttccga gtggccctgc tcgctagcac
      781 attcgcgacg ctgatcggcg gaactcttgg actggcccgc ctatcgccca actggttgtt
      841 ggccaaaatc gccgccggct acgtcgaact gctgcgaaat attcccttag tcgttcaatt
      901 gttcttctgg tacgcggtga tcacggaaag ccttccggcg ccagcagaag cactaaaccc
      961 ggttccgggg ttctatctca gcaaccgagg tctcgcggtg ccgactatcg tcacactgct
     1021 gccgttgcaa atcgaacatc caacgctcaa tgcgtttaat ttttccggcg gtgtcgtgtt
     1081 gtcgcccgaa ttcaccgcgc tcttcctcgg cttggctctt tatacagcgg cattcattgg
     1141 cgagattgtc cgcgccggca tccttgctgt cgatcgcggc caatacgagg cggcacaagc
     1201 gcaaggattg agccaggggc aaatcatgcg cttgattatt ttacctcagg ccctgcgcct
     1261 gatcgtgcca ccgcttacca gccaatactt gaactgcatc aagaatagtt cattggccgt
     1321 cgccatcggc tatcccgatc tagtttccat cgccaacacg gtgatcaacc aaaccggcca
     1381 ggccatcgaa ggcgttgcca tcatcatgct ggtctatctg accatcagcc tgacgatctc
     1441 tgctgcgatg aatttctaca accggcgtat cgctctgcgc ggtgccagaa catgattaca
     1501 cgcccgaggg taactattgc gcatcccgca gcgctgttgc attggacacg catccggctg
     1561 tttgccacct ggcgcagcag ctgcgttacg cttgccatcg tctatctcgc tgccaagttg
     1621 ctaccgccgc tcttcgactg ggcgttgctc cgcgccgtat ggtcaccgac ccatgccgcg
     1681 ctgtgtcgcg atacccgcat cgctggcgcc tgttgggcgt tcgtcgccga taactatcgg
     1741 tttattttgt ttgggacttt ccctgccgaa gagcaatggc gtccagcctt ggtgatcgtc
     1801 agtctgctcg cgctctacgc gttgagcggc tttcacctgc tgcgccggcc aacccttctc
     1861 aggctctggt tcggcgaact tcttgtgatt gccatgctca tgtggggcgg ggttttcggc
     1921 ctaacttacg tcgagaacga acgctggggt ggactgaccc tgacactttt actcagtact
     1981 ttcggcatcg ctttggcgtt tcccctggcg atcacattag ctttgggacg gcgctcagga
     2041 atgccgctga ttcgttggtt gtcgattggc tatattgaac tcatccgtgg tgtgccgctg
     2101 atctcggtgc tgtttatggc gtcggttatg ctgccattgt ttttgccttc gggagtttcc
     2161 atcgacaaac tcttgcgggc gcaggtggcg atgatcctgt tcgccgcggc gtatctcgcc
     2221 gaagtgattc gcggcggcct gcaagcaatc ccagcgcagc aagaagaggc cgccctcgcg
     2281 accggcttag ggtactggga ccgtgtccgt tacgtcatgc tgccgcaggc cttacgaatc
     2341 gccattccgc cgttggtcaa tacctttatc ggctttttca aggacacttc attggtcgtc
     2401 atcatcggcc tgttcgactt tcttacctcg atcaaggtct ctctcaccga accgacctgg
     2461 agcggtttcg gcgtcgaggc ttatctgttt gccgcccttg gctatttcgc attttgttat
     2521 ccgatgtcgc gctatagcca aaggctcgag cgccaaaccg gctatggcac gagcttgcgc
     2581 gcgctccgag acaattagtt accggtttgg gccgggcaag cggcctatgg tagaatttat
     2641 cagcgacgat gagaaaattg ctagtctgcc aacacgtgcc gcacgagatc ctcggcaccc
     2701 tcaaccccat gctcaagcgc gccggctttc gtatccgcta tgtaaatttt ggccgccatc
     2761 ccgacgccga accgaccttg gacggatacg acggcttggt ggtattaggc ggtccgatgt
     2821 ccgcgtatga cgaccctcgg ctgccccact tgacggtcga gatgaaactg atcgaaagcg
     2881 ccatgcaacg aaacctgccg gtcttgggca tctgtctcgg cgcgcaattg atcgcccgca
     2941 ctctcggggc gcgtgtctat gccaaccaca tcaaagagat cggttggtat tctgtcgcgc
     3001 cgtcggacca ggctgaacaa gacccgctgt tcagcgggtt cggcaaatcc gaaacgctct
     3061 ttcaatggca cggcgacacc ttcgacatcg ctaacggagc gatccatttg gcttggtcgc
     3121 gacactgcac aaatcaagca tttcgctacg gcaacaatgt ttatgctttt caattccatc
     3181 tcgaagtcga cgaaccgatg attctgcgct ggctgaaggt gccggagaat cgcaaagaga
     3241 tcgccagcct gcatggtgac atcgacccgg accagattca ccttcaaacc ggccgccaca
     3301 tagaccgcct gcatgagctg agcgaccgag tgttcggcga gttcatcgag ctgttcggtc
     3361 tcgacaaaga ccgccgccgg ctggtctcgc gatagaccgc ccgtttctac cacaaccgaa
     3421 cgtaagtgaa cttaggaaca gaacggttaa acagctttga acgaatgagc gggaatacta
     3481 atgggcggga tcgaaagcta cagaagaaat tttccgcgcg ggtttacttc actgctcagc
     3541 gcgtcatggc tcgggtcggt gcctgcgaac tgccacgagt cttgtttttt gctttcgcga
     3601 ggttattgtc gcgacaaaac aaccgagcga gatgaccgca gcaagcaccg ttctttcgaa
     3661 aatgttatac agcatggcag tgtcattagt gatgcggagc aacactgatg ccatcagtcg
     3721 atttgagaaa taaccgagag ttatgcagcg cctcaccaag cgacaggtga caggcgcggc
     3781 aggactgtca tggatgggtg gcgcctatgg ca
//
LOCUS       DATLCH010000177         2292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4188698, whole genome shotgun sequence.
ACCESSION   DATLCH010000177 DATLCH010000000
VERSION     DATLCH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188698"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..654)
                     /locus_tag="VMZ02_05245"
     CDS             complement(304..654)
                     /locus_tag="VMZ02_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71369.1"
                     /translation="MNTTKRTLLLGLLTAGTLLTAVPSDAAWHRDWQSGHSGYGYNYG
                     SRYGWGNDAREQREDLRELWRDRQELRRDLRRGASADEIARDRAEIRRDQQEIYSDRD
                     DGWRPWYRRWWWSR"
     gene            complement(798..>2292)
                     /locus_tag="VMZ02_05250"
     CDS             complement(798..>2292)
                     /locus_tag="VMZ02_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUR71370.1"
                     /translation="ATAAFSPDASPEFAGPASPGVLAVPHLLVVDDDPVVRQQLELFY
                     RQIGYGVTAVASAEEALEQFGTDAIDLVITDIKLPGMDGRKLISWVREHYPDIPMIAI
                     TGYSDIQMAVNVLKVGASDLIVKPFNLPAVQEATRAVFEKNQVYLDVRHLRRSLKNRS
                     VFSGMLSKTAEMHRVFEIIRVVGPTDMTVLVQGETGTGKELVASAIHYQSERRKAPFV
                     TINCAGFPESLLESELFGYEKGAFTGADQAKAGKIELADGGTLFLDEIESMSLVMQGK
                     LLRVLEDQRVQRLGSRSSIQVDMRVVAATNVPLEELVADGKMRADFYYRINVIPLHLI
                     PLRQRKDDIPLLVHDFVQRHPVANAKRINGISAEAMSALLEHSWPGNIRELQNVVERA
                     IVLSRGRIISEVDVPTLGRRTKLSPNSGARMAPLEPWLREQEKRYLTEQLELAGGNVT
                     LTAKACGIGLRTLSRKLQLHGLDHRFFRKKARMSVSPPPPGAGFLSV"
BASE COUNT          535 a          656 c          603 g          498 t
ORIGIN      
        1 cagatttgtc tcgattccga gtcggctgct tgtgtcacgc cgacgacagc tcagagtcac
       61 gagcgctcac aatcaaaaat aaaaatcggc aatatatatg ccaccagcta gaaagcatat
      121 gggatcgcaa ggagaggaga cgccttgagg gatttgcgtt tcggcaggag aatagaggcg
      181 ctgaataaaa aaaagggtcg gagctcccag tcgtcgacga ggttctaaac cttgcggcga
      241 cggggtgctc cgaccctgtg aatttgcgcc gttgaacggc tgctaagact aaattaactc
      301 agactagcgg ctccaccacc atctacgata ccagggacgc cagccgtcgt cgcgatccga
      361 gtaaatttcc tgctggtctc ggcgaatttc agcgcgatct cgggcgatct catcggcact
      421 tgcaccgcgt cgcaggtcgc gccgcaactc ctgacgatcg cgccacaact cgcgcaaatc
      481 ttcgcgctgt tcgcgcgcat catttcccca gccgtagcgg gacccgtaat tatagccata
      541 gccggaatgc ccagattgcc aatcccgatg ccaagccgca tcagagggta ccgcagtgag
      601 cagagtgccg gcagtcaata aacctaatag caacgtccgt tttgtcgtat tcataactcc
      661 tcctgtactg attggacgtg ccaatactca aaatcgttta ccccgataat ttgttgtttt
      721 ggcgagataa tattgctagg cgactgcgcg cgactttgtt aacaagaatg aaccagcaga
      781 ctcggcgaag tggagattta gaccgacagg aaccctgccc caggcggcgg cggagagacg
      841 gacatgcgcg cttttttcct gaagaatcta tgatccaaac cgtgcagttg cagtttgcgc
      901 gaaagagttc tcagcccgat accacatgct ttagccgtca gagtgacgtt tccgccggcc
      961 agctccaact gctcagtcaa gtaacgcttc tcctgttcgc gcaaccaggg ctcgagcggc
     1021 gccatccgcg cccctgagtt cggagacaat tttgttctcc gtcccagagt cggcacatcg
     1081 acttctgaaa taattcgccc gcgcgacaaa acaatagccc gttcgacgac gttttgcagt
     1141 tcgcgaatat tgcctggcca tgagtgctcc agaagtgccg acattgcctc ggctgaaatg
     1201 ccgttgatgc gcttggcatt ggcgactgga tggcgctgca cgaagtcgtg cacaagcagc
     1261 ggaatgtcgt ccttacgttg gcgcaacgga atcagatgaa gcggaatgac attgatccgg
     1321 tagtagaaat ccgcccgcat cttgccgtca gcgaccaatt cttcgagagg cacgttagtg
     1381 gccgccacga cgcgcatgtc aacttgaata ctcgagcggc tgcctaagcg ctgtaccctc
     1441 tgatcctcta acacacgcag cagcttgcct tgcatcacga gcgacatgct ttcaatctcg
     1501 tcaagaaaca aagtgccacc gtcggcgagc tcgatcttgc cagctttggc ttggtcggcg
     1561 ccggtaaaag cgcctttttc atagccgaac aattcacttt ccaaaagact ctcgggaaag
     1621 ccggcgcagt taattgtcac aaacggcgcc ttacgccgtt cgctttgata atgaattgcg
     1681 ctcgcgacaa gctcctttcc tgtgccggtc tcgccttgga ctaaaaccgt catgtctgta
     1741 gggccgacca cccgaatgat ttcgaagacc cggtgcatct cggcggtttt gctcagcatg
     1801 ccgctgaaaa ccgaacgatt cttgagcgag cggcgcaagt ggcgcacatc gagataaacc
     1861 tgattttttt cgaacaccgc ccgcgttgct tcttgcaccg ccggcaaatt gaacggcttg
     1921 acgatcaaat cgctggcgcc cactttgagt acgttcacag ccatctggat gtcggagtag
     1981 ccggtgatcg caatcatcgg tatgtcggga taatgctcgc gcacccagga aatcaatttg
     2041 cgcccgtcca ttccgggaag tttaatatcg gttataacga gatcgatagc gtctgtgcca
     2101 aactgttcga gcgcttcttc ggccgatgcc acggcggtga ctccataacc gatctgccga
     2161 taaaacagtt cgagttgctg acgcacgacc ggatcgtcgt cgaccactaa aaggtgcggc
     2221 accgcgagaa cgccaggcga agccggacct gcgaactccg ggctggcatc cggcgaaaaa
     2281 gccgcagtgg ct
//
LOCUS       DATLCH010000178         7818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1230415, whole genome shotgun sequence.
ACCESSION   DATLCH010000178 DATLCH010000000
VERSION     DATLCH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230415"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..410)
                     /locus_tag="VMZ02_05255"
     CDS             complement(36..410)
                     /locus_tag="VMZ02_05255"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HUR71371.1"
                     /translation="MQSNITQERVPIHGDAGHWLHISQSPRYVRVFFGGEVIASSKRA
                     KLVREAEVLPAYYFPKEDVRTDLLTVSSHQSDCPVKGSASYWSINSGGRSVENAVWSY
                     VKPLPAAVEILNHFAFDRKRSD"
     gene            complement(594..1547)
                     /locus_tag="VMZ02_05260"
     CDS             complement(594..1547)
                     /locus_tag="VMZ02_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HUR71372.1"
                     /translation="MAVTIGSGDFRYEVIESFGKLPDDWTFHEVAAVAVDKKDQLYCF
                     TRGKHPVIVFDRDGNFLRSWGDGVFKRAHGATIAADDTIFLTDDGDHTVRKCSLDGKI
                     LMTIGVPEKSAPYQSGEPFNRCTHVAFSPQGDIYVSDGYGNSRVHKYSPDGKLLLSWG
                     ESGSDPGQFNIVHNIVTDKDGWVYVADRENNRVQVFDGNGKYETQWNNMHRPCALYMD
                     TTKKDPLCYVGELGPGLGVNLNAPNLGNRVDIYDKHGKRLARLGDIFAGEKPGQFIAP
                     HGIAIDSRGDIYIGEVSWTIMGQRLQPPRELRSLQKLKKLN"
     gene            complement(1603..1917)
                     /gene="glpE"
                     /locus_tag="VMZ02_05265"
     CDS             complement(1603..1917)
                     /gene="glpE"
                     /locus_tag="VMZ02_05265"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HUR71373.1"
                     /translation="MEITQIQIDEAKKKLDEKNCLFVDVRDPGSYQQAHIPGALHLHD
                     GNVQEFLQNTAKDQNVIVYCYHGNSSMGAAGYFLENGFTNVASMMGGFEAWRQVYEHE
                     AG"
     gene            2033..3226
                     /locus_tag="VMZ02_05270"
     CDS             2033..3226
                     /locus_tag="VMZ02_05270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71374.1"
                     /translation="MTPSPLALYTLAAIFQTAGSSGAAMLAPFFMKEHGYSIAIASMP
                     LVANGLGRVCSDLLSGVMATYFSPGLLLFAAMILGLATSIVGYLFMDIMPVFLTVWIL
                     FGLTEAMFALSLRKIGFDQSPPGRQGRVQGQMASALGLGFTLGPLLGGFIGKWLGPNG
                     LFFLYAMPQLIGMIIVALAGAHRSRRKDRSITPNLWREGRLLLRKVPFLASCLAICQS
                     FLFLVGVTRVAFPFLAVNLRAMSLETVGTIVSISRLTDTLGRYLGGRLCDRIKPARVI
                     LFGVAIGIPMFLLQPYGSGFVTLLIPLAILTMGFGFTNVGATTFALQAAGENTKELSL
                     GISRASTSVGNMLGPLLAGVLVESAGYEHSFHAMSAISLAVLFLTWAGLKQKPADGVN
                     VAGRL"
     gene            complement(3254..4045)
                     /locus_tag="VMZ02_05275"
     CDS             complement(3254..4045)
                     /locus_tag="VMZ02_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HUR71375.1"
                     /translation="MAERVWEKFLTEQDKATLTGKTDRRIGFGERPALLLIDLYRWVF
                     GDKPEAVTDAIKTWPGSCGMAGWNALPHIQKLLAKCREVGIPIIHVTGMDGVAVEPWA
                     FRRDTKKRATMGADELDRFRRRYDIVEEVKPIPGETVFKKVSPSAFWGTPLIGHLNFL
                     GVDTIITCGESTSGCVRASVVDGTTNRLRMVVAEECVFDRHEACHAINLFDMNQKYAD
                     VLPLDDIIKYLDGWRAEKAGQVGYANNSMEYLKDLAIGEDYKGLR"
     gene            4444..4836
                     /locus_tag="VMZ02_05280"
     CDS             4444..4836
                     /locus_tag="VMZ02_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71376.1"
                     /translation="MPTRRQYKKKPTSVITAVQFDLDSEGFVYNKWGGPQRCKRGDWL
                     VDNAGDKYTVAQESFAKTYEFVSPGVYTKSTSVWAEVADKPGKVKTSEGESAYEAGDY
                     LVSNNQDGSDSYAISAAKFRDLYEIAED"
     gene            complement(5032..7236)
                     /locus_tag="VMZ02_05285"
     CDS             complement(5032..7236)
                     /locus_tag="VMZ02_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUR71377.1"
                     /translation="MPKASDDALEYLGGAAQLFEVVNKLQKQLKSANEINLARRVIAK
                     MRKEEDLLDELPPIHKADLCRQHAELTSKDPELSVNSRHDIALKILGEVFDLGNVSLN
                     GDAETLGTAGGICKRRWMDLGQYEDLQRAAEYYRRGALGPVGDDGYAQINAAFMQDLL
                     GHASATDGPHQQEADLIREKILGELPPLDKVKEENQWWNAATRAEALFGVERYGEAAA
                     ALKASSRRPEIWRLQSTAQQFALLAQLRNKRPLQNPELRAVFEALLPGAAQAVSSALI
                     GKVGLALSGGGFRASYYHLGVLARLAELDVLRHVDVLSCVSGGSIVGACYWMMLRNRL
                     LKAAPMAQADYISLVHELIAHFDRAVAQNLRGQPSKMAVGWRFLTGAKGALDTEKIAA
                     ALEKYFYGPLWHESKPLFMHHLAFKPVDHDPVLAGEEDFNPAKHNWLRSDNVPVLVLN
                     ATTVNTGHAWQFTPTWMGESPWSTQPAADSVPRLQWHWYDPGSKWEMELARAVSASAC
                     VPGLFEPLRIDDAYEGVQAQLIDGGVHDNQGTLALLAHNCRVLIVSDAAGQLLLEQSP
                     TPGLGGLTSYAARAMDTLMERVRLANYADLAARVRTKLVRGLMFLHMKDGLDADVIRL
                     KFSQESYDLRRNSLSPLGVRKDFQKALAELRTDLDAFSDVEANALMACGYRMAATAFE
                     RDLRQFDDLYAQPVPGKWPFDAMLQEIKSTAASTPNRESLLNALRAGSKRTV"
     gene            7416..>7818
                     /locus_tag="VMZ02_05290"
     CDS             7416..>7818
                     /locus_tag="VMZ02_05290"
                     /inference="COORDINATES: protein motif:HMM:NF017830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71378.1"
                     /translation="MEKLTPIDLAGIIETVKAEGKTRRVLWQDSESIAFLSSGRKERW
                     DFHIDPADEVTLQLTGVQHLVYRTADGKEQTADIKAGQILLCPAGVPHSPRLEEDSWF
                     IVFERKRQSGEIDQFKWFCNKCGENVYETSVE"
BASE COUNT         1692 a         2235 c         2088 g         1803 t
ORIGIN      
        1 ttatgtgaag ccgctgccgg cggcagttga aatcctcaat cacttcgctt tcgatcgaaa
       61 gcgaagtgat tgaggatttc aactgccgcc ggcagcggct tcacataact ccacacggcg
      121 ttctccactg acctcccgcc ggagttaatc gaccaataag aggctgagcc tttgaccgga
      181 caatcggatt gatgcgaact cacagtgagc agatcggtgc gcacatcttc cttggggaaa
      241 taataagccg gcaacacttc cgcttcgcgg acgagcttgg cgcgcttgct gctggcaatg
      301 acctcgccgc caaagaacac ccgcacataa cgtggactct gcgagatatg cagccaatgt
      361 cccgcatcgc catggatcgg cacacgctct tgagtgatat tgctctgcat gatcatcgcc
      421 tccgtttgac cctcactatc atgggtgaag tttaaccccc accttcatcc tcccccgcaa
      481 gcgggggagg aaacaggagg gggatttgtg acctttgcgt tctttgcggc caacattccc
      541 aatccgaatt cactacttcg cgtccttcgt gccttcgtgg tgcaattatc ctctcaattc
      601 agtttcttca acttctgcaa gctgcgcagt tcgcgcggcg gctgcaagcg ttggcccatg
      661 atcgtccagg acacttcgcc gatataaata tcgccgcgcg aatcgatggc gatgccgtgc
      721 ggcgcaatga actggccggg cttttcgccg gcgaagatat cgcccaagcg cgccaaccgc
      781 ttaccgtgct tgtcataaat atcgacgcga ttcccgagat tgggcgcatt taaattcaca
      841 ccaagtcccg gaccgagctc accgacatag cacagcggat cttttttggt cgtgtccatg
      901 tagagagcgc aagggcgatg catgttgttc cactgggttt cgtatttgcc gttgccgtca
      961 aatacctgaa cgcggttatt ctcccgatcg gcaacgtaga cccaaccgtc tttgtcggtg
     1021 acgatattgt gaacgatgtt gaactggccg ggatcggagc ccgattcgcc ccaggagagc
     1081 aacaactttc catccggcga atatttgtgc acccgcgaat taccgtagcc gtcggaaacg
     1141 tagatatcgc cctgcggcga gaacgcgacg tgcgtgcagc gattgaacgg ttcgccgctt
     1201 tgataaggtg ccgacttctc cggcacgcct atggtcatga ggattttgcc gtcgagcgag
     1261 cacttgcgca ccgtgtggtc gccatcgtcg gtcaaaaaaa tagtatcgtc ggcagcgatg
     1321 gtcgctccgt gagcgcgctt gaacacgcca tcgccccagg agcgcaggaa gttgccgtcg
     1381 cggtcaaaaa cgatgaccgg atgtttgccg cgggtgaaac aatagagctg atctttcttg
     1441 tcgaccgcca ccgccgccac ttcatgaaat gtccagtcat ccggcagttt gccaaagctc
     1501 tcgatcactt cgtagcgaaa atcaccgctg ccaattgtca ctgccatgga tgcctcccga
     1561 gctattcgat cttcgccctt ctatcttcca ttgtcggccg actcatccgg cctcatgctc
     1621 gtagacctgc cgccacgctt cgaaaccacc catcatgctg gcgacgttgg taaagccgtt
     1681 ttcaagaaaa tagccggcag cgcccatgct gctgttgccg tgatagcagt agacaatcac
     1741 gttctgatcc ttggcggtgt tctgcaagaa ttcctgcacg ttgccgtcgt gcaaatgcag
     1801 cgctccggga atatgcgcct gttggtacga gccgggatcg cgaacgtcga cgaatagaca
     1861 attcttttca tcgagtttct ttttcgcttc atcaatttgt atctgggtaa tttccatcgc
     1921 tccctcctcg cttgatgcgg tctttctatc aagctcgttt cagcgtgtcc atcacgttaa
     1981 ttaaccacgc ttgcatggga tcaccacaat tctgctgcta taatttcgcg ccatgactcc
     2041 atcccctctc gcgttgtaca ccttggcagc gatttttcag accgccggtt cctccggtgc
     2101 ggcaatgctc gcgccgtttt ttatgaagga gcacggctac tcgattgcca tagccagcat
     2161 gccattagtc gccaacggtc tagggcgggt atgctccgat ctcttgtcgg gggtcatggc
     2221 tacctatttc agtcccggat tgcttctatt cgcggccatg attctcggac tcgcaaccag
     2281 catcgtcggt tacttattca tggacatcat gccggtgttt ttgaccgtat ggattttgtt
     2341 cggcctgacc gaagcgatgt tcgctttgtc tttgcgcaag atcggttttg atcagtcgcc
     2401 gcccgggcgc caaggtcgag tgcaaggcca aatggcttct gcgctcggcc tgggcttcac
     2461 gctcgggccg ctgctcggcg ggtttatcgg caaatggctc gggcctaatg gattattttt
     2521 tctttacgcc atgccacagc tcatcggcat gatcatcgtc gcgctcgccg gcgcgcaccg
     2581 atcccggcgc aaggaccgca gcatcacgcc aaatctatgg cgcgagggcc gattgttact
     2641 ccgcaaggtt ccatttctcg cttcgtgttt agccatctgt cagtcatttc tgttcttggt
     2701 cggtgtgacg agggtcgctt ttccgttcct tgcggtcaat ctgcgcgcta tgagcctcga
     2761 aactgtcggc acgatcgtgt cgatttctcg attgaccgat actctaggcc gttatttggg
     2821 cggccggctt tgcgaccgga ttaaaccggc gcgagttatt cttttcggtg tggcgattgg
     2881 gatcccgatg tttttgctgc agccctacgg cagcggcttc gtcacactct tgatcccttt
     2941 ggcgatctta accatggggt ttggttttac taacgtgggc gcgacaactt tcgctttaca
     3001 agctgccggc gagaacacca aggaactgag tctcgggata tcaagagcat cgacgtccgt
     3061 cggtaacatg ctcggaccgt tgcttgcagg ggttttggtg gaaagcgcgg ggtacgaaca
     3121 ctcttttcat gcaatgtcgg cgatatctct ggcggtgctg ttcctaacct gggcaggatt
     3181 aaaacaaaag ccggctgacg gcgtaaacgt cgccggccgg ctttgatgcg gtgagccgaa
     3241 tgccgcgctc ctactagcgc aatcccttgt agtcctcgcc gatcgcgagg tctttcagat
     3301 actccatgct gttgttcgcg tagcctacct gaccagcctt ctctgcgcgc cagccgtcga
     3361 ggtatttaat gatatcgtcc agcggcaata cgtcggcgta tttctgattc atatcgaaca
     3421 aattgatcgc atggcaagct tcgtggcggt cgaaaacgca ttcctcggca acgaccatgc
     3481 gcaaccgatt ggtcgtgcca tcgaccacgc tggcgcgcac gcaaccgctg gtgctctcgc
     3541 cgcaggtaat gatcgtatcg acacccaaaa agtttaggtg accgatcaac ggcgtacccc
     3601 aaaaggcgct gggagaaact tttttgaaca cggtctcgcc gggaatcggt ttaacttctt
     3661 cgacaatatc gtaacggcgg cggaaacgat ccaattcgtc ggcgcccatg gtcgcgcgct
     3721 tcttggtatc ccggcgaaat gcccaaggct cgacggcgac gccatccatg ccggtcacat
     3781 gaatgatcgg aatccctact tcgcgacact tggcgagcag cttctggata tgcggcaacg
     3841 cgttccagcc tgccatgccg cagctcccgg gccaggtttt gatcgcgtcg gtcaccgcct
     3901 ccggtttgtc gccgaacacc cagcgataaa gatcgatcaa caacaaggcc ggtcgctcac
     3961 cgaaaccgat ccgtcgatcg gttttcccag taagcgtcgc cttgtcttgc tccgtaagaa
     4021 acttttccca aacacgctca gccatgcagc acctccgatt agaattggtt aagccttatc
     4081 atcgcgctgc ttccatcgtc aacatagccc gggtaatggt tcagtttgag tcgggcggac
     4141 gttttcaatc tgaggaagat ttctcgcttc gctcgaaatg acagcagaat agtcgccgtg
     4201 tcatctcgaa cgaagtgaga gatctatttc taactgaacc actatccaga gcccagggga
     4261 aggtggatcg aagcgaaaga gcatcgctgt gacgagctct ccgagaggcg agaaggccaa
     4321 tctgacagcc aagccatatc gtgacattgc atgcaaatct ggttatatac tctggagcaa
     4381 agtcgcccag cgcttcaccg cgcgagcatg aacattgaac gaaccgtcaa aaaggagcaa
     4441 tcgatgccga ctagacgtca atacaaaaag aagccgacat ccgtcatcac cgcggttcag
     4501 ttcgatctcg acagcgaagg atttgtttac aataaatggg gcggaccgca acgctgcaaa
     4561 cgcggcgatt ggctcgtcga caacgccggc gacaagtaca ccgtcgcgca ggagtcgttc
     4621 gccaagactt atgagttcgt cagccctggt gtttatacga aatcgacatc cgtctgggcc
     4681 gaggtcgcgg ataagccggg caaagtcaaa accagcgagg gtgaatcggc ctacgaggcc
     4741 ggcgactacc tagtatcgaa caaccaagac ggcagtgatt cctacgccat cagcgcggca
     4801 aagtttcgcg atctttacga gattgccgaa gattagcgcg agacggcttc gctgggttcg
     4861 aaaaacaatt tctgtttcat aagacggcct caagaacggg acattaccca ccaagcagtg
     4921 aattgtctgc gctctcgcac ggagcctcaa atagttgcgc cactccctta gttaaattgg
     4981 atagcgggaa gatcgatggt cttgaaagta tgaacgttag ttagagcgcc ctcagacggt
     5041 tcgcttgcta ccggcgcgta gtgcattgag caaactttcc cgattgggag tggaagccgc
     5101 ggtcgattta atttcctgga gcatggcatc gaacggccat ttgcctggga ccggctgagc
     5161 atacaaatcg tcgaattgtc gcaggtcgcg ctcgaatgcg gtagctgcca tgcgataacc
     5221 gcaggccatc agcgcattcg cttcaacgtc ggagaatgcg tccaggtcgg tgcgcagttc
     5281 cgccagggct ttttgaaaat ccttgcgcac gccgaggggt gagagcgagt tgcggcgcaa
     5341 atcgtaagat tcctgggaaa acttgagtct gataacatcg gcatccaagc cgtctttcat
     5401 gtgcaggaac atcaacccgc gcactaattt ggtgcgcacc cgagctgcaa ggtcagcata
     5461 attcgccagc cgcacgcgct ccatcagggt atccattgcg cgtgccgcgt aactggttaa
     5521 acccccgagg cccggcgtag gtgattgctc gagaagcagt tgccccgccg cgtcactgac
     5581 gatgagcaca cggcaattat gcgccaacaa cgccagggtg ccttgattgt catgaacacc
     5641 gccgtctatc aactgcgcct gaacaccttc ataggcatcg tcaatgcgca gcggttcaaa
     5701 gagtcccggc acacaagcag acgcagatac cgcgcgggcg agctccattt cccacttcga
     5761 gccaggatcg taccaatgcc attgcaggcg cggcacactg tcagctgcgg gttgagtcga
     5821 ccatggcgat tcacccatcc aggttggcgt gaactgccag gcatggccgg tattcactgt
     5881 cgtagcgttc aggaccagca ccggtacatt gtcgctgcgc aaccaattgt gcttggccgg
     5941 attgaaatcc tcctcgcccg ccaagactgg atcgtgatcg acgggtttaa acgcgagatg
     6001 gtgcataaac agcggtttgc tctcgtgcca gagcggacca taaaaatact tttccagggc
     6061 cgcggcaatt ttttccgtgt cgagggctcc cttggcaccg gttaaaaagc gccagccgac
     6121 agccattttg gatggttgtc cgcgtaggtt ctgggcgacc gcgcgatcga aatgtgcgat
     6181 cagctcatgg accaatgaaa tataatcggc ctgcgccatg ggcgcggcct tgagcaggcg
     6241 gttacgcaac atcatccagt aacacgcacc gacaatactg ccgccggaaa cacacgagag
     6301 cacgtcgacg tggcgcaaaa cgtccagttc ggcgagccga gccaaaacac caagatggta
     6361 atacgatgcc ctgaatccgc cgccggagag cgctagtcca actttaccga ttaacgctga
     6421 actcaccgct tgtgccgctc ccggcagcaa cgcctcgaaa acagcgcgca actccggatt
     6481 ctgcaatggg cgcttgttgc gcaactgggc tagtagcgcg aattgctgag cggtgctctg
     6541 caagcgccaa atctccggcc gcctggatga ggccttgagc gctgccgccg cttcaccgta
     6601 acgctccact ccgaacaatg cttcggcgcg cgtggcggca ttccaccatt gattctcttc
     6661 tttaacctta tctagagggg gcagctcacc aagaattttt tcacgaatca gatctgcttc
     6721 ctgttggtgc ggaccatccg tcgcggaagc atggccgagt aaatcctgca taaacgcagc
     6781 gttgatctga gcatagccat cgtctcccac cggaccgagg gcgccgcgcc ggtaatactc
     6841 agcggcgcgc tgcaaatcct cgtactgacc caagtccatc cagcgccgtt tgcagattcc
     6901 gccggcggtt cccaaggttt ccgcgtcgcc atttaaactg acatttccca ggtcgaacac
     6961 ttcgccaaga attttgagag cgatatcgtg acgcgagttg acgctcagtt ccggatcttt
     7021 gctggtcaat tcggcgtgtt ggcggcacag atcagccttg tgaatgggcg gtagctcatc
     7081 gagcaggtct tcctctttgc gcatcttggc gatgacgcgc cgcgcgaggt tgatttcatt
     7141 tgcagatttg agttgttttt gcagcttgtt gacaacttca aacagctgcg ccgcgccgcc
     7201 cagatattcc aacgcgtcat cacttgcctt cggcatggta catcctatct cgcccagtga
     7261 atgtgacaac ttgggattgc cgggtcgatt gaagcaggta catcgattat cattttatac
     7321 atcgtctctc tggaaaaaat caaagttaaa gtgatcgcga gctcgaacag tgactcggtt
     7381 gccgagtcgg tttctaaagc gttatagtcg accccatgga aaagcttacc ccaatcgatc
     7441 tcgctggcat catcgagaca gtcaaagccg aaggcaagac ccggcgcgtt ttgtggcagg
     7501 actcggaatc catcgcgttt ttaagcagcg ggcgaaaaga gcgctgggat tttcatatcg
     7561 atccggcgga cgaagtgact ttgcaattga ccggcgtgca gcatctggtc taccggacgg
     7621 ccgatggcaa agagcaaacc gccgacataa aggcggggca aatcctgctc tgccccgccg
     7681 gcgtgccgca ttcgccgcga ctcgaggagg actcctggtt catcgtcttt gagcgtaagc
     7741 gtcaatccgg tgaaatcgat cagttcaaat ggttctgcaa taaatgcggc gagaacgtct
     7801 acgaaacttc cgtcgagg
//
LOCUS       DATLCH010000179         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9425164, whole genome shotgun sequence.
ACCESSION   DATLCH010000179 DATLCH010000000
VERSION     DATLCH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9425164"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VMZ02_05295"
     CDS             <1..1041
                     /locus_tag="VMZ02_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HUR71379.1"
                     /translation="KACVGLNPPQRVWCHITGTDLVRDRDGRVYVLEDNLRCPSGVSY
                     VLENRRIMKRTFPQVFEASRVRPVDEYPSRLLSTLQSLFPGAFDSPKVAVLTPGIYNS
                     AYYEHSFLAQQMGVELVEGRDLVAVNNRIMMRTTKGLQPVDVLYRRIDDDFLDPQVFR
                     ADSLLGVPGLMDIYKKGRLALANAPGTGIADDKSIYAYVPKIIEYYLGEKIIIPNVPT
                     YICAEANDLSYVADHLDQLVVKAVNESGGYGMLVGPHATAQERSEFARRIRNDPRNYI
                     AQPTLGLSRVPVIAEGRIVGRHVDLRPYVLYGSDIYVLPGGLTRVALKAGSLVVNSSQ
                     GGGSKDTWVLQS"
     gene            1070..2047
                     /locus_tag="VMZ02_05300"
     CDS             1070..2047
                     /locus_tag="VMZ02_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HUR71380.1"
                     /translation="MLSRVANSIYWLSRYMERADNVARFIDVNLQMMLDLPSGSQEQW
                     QPLVSISGDDGAFSSIYPVATRDNVIKFLAFASDNPNSIVSCLRAARENARTIRESIS
                     PEMWEQVNSCYLMVNAAASNELPGSSSSFFSDVKKASQLFHGVTDATLLHGEEWHFYR
                     VGRLLERADKTSRLLDVKYFMLLPSVTDIGTPLDDIQWAAVLRSTSALEMYRKRHGHI
                     APEQIIQFLVLDHEFPRSIHYCLTTANDSLHAISGTPIGMFRNAAEQRLGQLRAELAY
                     TDTRQIVTRGLHEFLDAFQTKLNLVDQSIFDAFFALRPVASNAQDQPEQ"
     gene            complement(2222..>2579)
                     /locus_tag="VMZ02_05305"
     CDS             complement(2222..>2579)
                     /locus_tag="VMZ02_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71381.1"
                     /translation="VTAVAKEKGWKMRDFELAYNCVLARGLVNEWTWKRPIANKSRTA
                     TAEVHIEHDITEARLLVRFRDRNKTIIKVKPLVSGLPSEFVFYSYLGKLRWRDDVTVE
                     LISREGDNRYRADFKV"
BASE COUNT          557 a          713 c          707 g          602 t
ORIGIN      
        1 aaagcatgcg tcggcttgaa tccgccgcag agggtatggt gccacatcac cggcaccgac
       61 ctggtgcgcg accgcgacgg gcgagtttac gttttggaag acaatctgcg ctgtccgtca
      121 ggcgtgtctt acgtcctgga aaatcgccgg atcatgaagc gcactttccc gcaggtattc
      181 gaggcttcac gggtcaggcc ggtggacgaa tatcccagcc ggttgctctc gacccttcaa
      241 tcgttgtttc caggggcttt cgactcgccc aaagtagccg tgctgacgcc ggggatttac
      301 aactcggcgt attacgaaca ctcctttttg gcgcagcaaa tgggcgtgga attggtggag
      361 gggcgcgact tggtggcggt caataaccgg atcatgatgc gcacgaccaa agggctccag
      421 ccggtggatg ttctgtaccg gcgcatcgac gatgattttc tagatccgca ggtgtttcga
      481 gccgattcgc tcctcggtgt tcccggactc atggatatct acaaaaaggg acgtttagcg
      541 ctggcgaacg cgccgggaac cggcatcgcc gacgacaaat cgatctacgc ctatgtgccg
      601 aaaataatcg aatactacct tggcgaaaaa ataattattc ccaacgtgcc gacttacatc
      661 tgcgccgaag ccaatgacct gtcctatgtc gccgatcatt tggatcagct ggtggtcaaa
      721 gccgttaatg aatccggcgg ctacggcatg ctcgtcgggc cgcacgcgac ggcgcaggaa
      781 cgcagcgagt ttgcccggcg gattcgcaac gatccgcgca actatatcgc acagccgaca
      841 ttgggcttat cgcgggtgcc ggtgatcgcc gaaggtcgca tcgtcgggcg tcatgtcgat
      901 ttgcgtccgt atgtgcttta cggcagcgac atttacgtgc tgccgggagg cctgactcgg
      961 gtcgcgctca aagcgggttc gttggtagtc aactcgtcgc agggcggcgg gagcaaagat
     1021 acctgggtgt tacagtcgtg aatcgcgccc taatcagcaa ggcgccgaga tgctaagccg
     1081 agttgcaaat tcgatctact ggttgagccg ctacatggaa cgggccgaca atgtcgctcg
     1141 cttcatcgac gtcaacttgc aaatgatgct cgatttgccg agcggttcac aggaacaatg
     1201 gcagccgctg gtgagcatct ccggcgacga cggcgcattc tcgtcgatct atcctgtcgc
     1261 tacgcgcgac aacgtcatca aatttcttgc ctttgccagc gataacccaa actcgatcgt
     1321 gtcttgtttg cgcgccgcgc gggaaaatgc ccggacgatt cgcgagtcga tttcgcccga
     1381 gatgtgggag caggtcaaca gctgctattt gatggtcaac gccgcggcgt caaacgaatt
     1441 gcctggttcg tcctcgagct ttttcagcga cgtgaaaaaa gccagccaac tgtttcacgg
     1501 cgttaccgat gcgactctat tgcatggtga agagtggcat ttctaccggg tcgggcgact
     1561 gctcgagcgg gccgacaaaa cctcccggct gctcgacgtt aaatatttta tgctgctgcc
     1621 ttcggtcacc gacatcggca cgcctctaga cgatattcag tgggccgcgg tgctgcgatc
     1681 gacgagcgcg ttggaaatgt accgcaaacg ccacggccat atcgcgccgg aacagatcat
     1741 ccagtttctc gttttggacc atgagtttcc acgctcgatt cattactgcc tgacgaccgc
     1801 aaatgactcg cttcacgcga tttccggaac gccgatcggg atgttccgca acgctgccga
     1861 gcagcgcctg ggtcagctgc gcgccgaatt ggcttatacc gacacgcgcc agattgtcac
     1921 gcgcggcctg catgaattcc tcgatgcgtt tcagactaaa ttgaatttag tggaccagag
     1981 tatttttgac gctttttttg cgctccgccc agtggcatcg aatgcgcagg atcaacccga
     2041 gcagtgagcg acgggtaaat cggcctcgac agaccaggcc aaaacgcaac gaagccaatc
     2101 cagatgggat ggcgttcaga attcgaagta cttcgattcc aagctcgagc cacggcccag
     2161 cggatcgagg acgacattga tttgctgcga tcgctttatc ttggcggatc tgtccgagtg
     2221 gctatacttt gaaatcagct cggtatcggt tgtcaccttc tcttgatatg agttctacgg
     2281 tcacatcatc gcgccatcga agtttcccca aatatgagta aaaaacgaac tcacttggga
     2341 gacctgatac cagcggtttt actttaataa ttgtcttatt tcgatcacgg aatctcacca
     2401 acaaacgggc ttcagtgata tcgtgttcga tatgtacttc agcagttgca gttctggatt
     2461 tgtttgctat tggacgcttc catgtccact cgtttacgag accgcgagca aggacacaat
     2521 tgtaagccaa ttcgaagtcc cgcattttcc accctttttc cttcgctaca gccgttacc
//
LOCUS       DATLCH010000180         8586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6204441, whole genome shotgun sequence.
ACCESSION   DATLCH010000180 DATLCH010000000
VERSION     DATLCH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6204441"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VMZ02_05310"
     CDS             complement(<1..1281)
                     /locus_tag="VMZ02_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HUR71382.1"
                     /translation="MRTLIANALIVTMNDHNDIVEKGAIVVDDNRITYCGPAEWTPAG
                     PFDRTIDASRMIAMPGMVNAHCHSPANLVRGMMPSKPLEIWRAYYRASLRDMREGDFY
                     ASALLGGIEMLMNGATTVLDHFAGSAACRFMGAGAAIQAMRDLGLRHVAALTVTDKNY
                     EDTIPLGHTDAALNNEMMRMSASEAKTAKAWLDECEEFIETFHAPEKLTTACPGPSAV
                     QRCSDELLKGCAEIARKKNLPLHIHLAETKAQQVQGNSIYGHSLLRHLESLGLVGENL
                     SCAHSIWIEDDDVELFAKQGATPVHNPASNLRIGSGLAKIKEFLAAGVHVGLGTDGAA
                     SNDGQNMFDALRLAALIHNQAGSDFNRWVTPAQALAMATRNGAHAFGLDAGIIAPGKL
                     ADIVLLRRDSPAYRPLNNVVNQIVWCENGSSVDTV"
     gene            complement(1468..2136)
                     /locus_tag="VMZ02_05315"
     CDS             complement(1468..2136)
                     /locus_tag="VMZ02_05315"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HUR71383.1"
                     /translation="MSEVSKKVAEMLLSTESIAVYKDKPYVFVSGRISPVYIDCRKLL
                     SFAAEREYIVTALAKMTETDIGLDNFDVVAGGETAGIPYAAFVAHIVKKPMIYIRKQP
                     KGYGGTKQIEGILDPGKRVLMVEDLITDGLSKLRFNIGVRGAGAKMTHCLCVFDYASD
                     RLDQHEGRDTLAKNGIALHVLANWDDVLDTGTSKNYFTAEQNTHIIDFLRDPENWGRK
                     MGFV"
     gene            complement(2418..2540)
                     /locus_tag="VMZ02_05320"
     CDS             complement(2418..2540)
                     /locus_tag="VMZ02_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71384.1"
                     /translation="MSKGMDSKKQGKKKPLKTLMEKRADKRAKKETKGFSLKVK"
     gene            complement(2628..2972)
                     /locus_tag="VMZ02_05325"
     CDS             complement(2628..2972)
                     /locus_tag="VMZ02_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBPRA1643 family SWIM/SEC-C metal-binding motif
                     protein"
                     /protein_id="HUR71385.1"
                     /translation="MSNRITDIGIMRSSVDADSRINTFGAKKTAKLGSEKNPASVRVQ
                     SAARSKEVRETFEKHGWEYRISIEPDEAEDLSDLKRLLNPQKPTVAEKKVGRNEPCLC
                     GSGKKFKNCCAK"
     gene            complement(3083..4072)
                     /locus_tag="VMZ02_05330"
     CDS             complement(3083..4072)
                     /locus_tag="VMZ02_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71386.1"
                     /translation="MGAIRLLLIALVIGWGAPVCAEPIRIAYSGVSASGTPVWLAKEE
                     GLFAKHGLEADLVAVRSAPIQVSALVSNEVQFVRGSASSMLSAAAQGAKLKIIMSLFA
                     ERASYDFLVAPAVTKPGDLRGKKIGVQDFSGLLWSLTMLSLREMGLDPQRDNISIQAI
                     GDSTVIAQSLASGIIDGAALDKLQSARLQTLGVKPLLDLSKIALPSSPFMCAETFVQK
                     HPQTIENFIKAMIEASAFMRANKERGIALLQKYLKVDRQLAELGYKFLLDDLTSYTFV
                     TPKGLKTAQDIISMRDPKIVKFNVEELLDQRVLTKVVKSGYVEDIERRYKLRP"
     gene            4236..5528
                     /locus_tag="VMZ02_05335"
     CDS             4236..5528
                     /locus_tag="VMZ02_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HUR71387.1"
                     /translation="MKRPPTAVRPSSLPAINQRRLMRDLNSIGRIGIGDHGGVTRLVF
                     SIKELRSRQVLIHLMRQAGLKIHIDAIGNIFGRLEGHSPKAPAVLSGSHLDTVIHGGK
                     YDGPVGVIGALEAVRTINENRVPIHSPLEVVCFVGEESSRFGFSTLGSSRVAGEVHAK
                     DLANAVDAQGTKLADVLASLGIARKNLGSLARNPKSLKGYLELHIEQGPILEALGKRI
                     GLVTSIAAPTRFKVIFTGQADHSGTTPMEMRKDALVAAARLIEFVEKTCRRFSAMAKG
                     RVVGTVGALKVEPGVINAVPGKTELLVDIRAITAQAKDRVVREVRAEAQRIAHRRAIA
                     VEILTIREEDPVPLNHRFLQLTKKICAEKNIVYEIMPSGAGHDAMQMAKITPAAMIFI
                     PSQRGISHNPLEWTDAEDIGLGAQILMETMVRVANEKF"
     gene            5515..6057
                     /gene="pyrR"
                     /locus_tag="VMZ02_05340"
     CDS             5515..6057
                     /gene="pyrR"
                     /locus_tag="VMZ02_05340"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HUR71388.1"
                     /translation="MKNFKAKKKRILTAVQIAAIFERFASEMAKTYESLEHVVFIGVR
                     TRGPYVAKRLAAMIKKRHKNEIPVGEMDITLYRDDLNALLPGGTIDHTRIPFDIGNKI
                     VVLFDDVLNTGRTARAAVDHLIDLGRPRMIHLAVIIDRGGRELPIAANYVGKTIRLKS
                     GGNVEVRLKEVDKVDEVLLG"
     gene            complement(6347..7468)
                     /locus_tag="VMZ02_05345"
     CDS             complement(6347..7468)
                     /locus_tag="VMZ02_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR71389.1"
                     /translation="MDASKPNILVVDDEMGPRESLKMILNPYYNVLVADRGAQAIEML
                     KEHSVDLVTLDLKMPGLSGINVLEKVKAHDPDIEAIIITGYGSLDTAIEGLRLGAFDY
                     ISKPFDVNHILSLVRRGLERRTAKAKLRQVKSDFLSNISHELRTPLSVVVGFVYLLLN
                     QVIGKLSEEQQKVLETVYRNSEELLELIDNVLWMTSLNAGDTSAIVEKFDAREVVKEA
                     VKRHEKMIRDKGLQLDVQFSDGDLSMVNDRAKVERVFQNVFNNAVKFTAQGQITVKVD
                     TSPDRTHMDFEVRDTGVGIEQNKIDTIFEPFHQSDNSTQRAFSGLGIGLTVARRMADL
                     VGGKLSVTSQPNAGTQVFMSFPAQVVVAAPLAVEQRKYA"
     gene            complement(7589..>8586)
                     /locus_tag="VMZ02_05350"
     CDS             complement(7589..>8586)
                     /locus_tag="VMZ02_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUR71390.1"
                     /translation="GIDFIIGKSRQITDVFKAVSMVAAKKSTVLITGESGTGKELIAR
                     AIHYNSDRRSKPFVVVNCAALPETLMESELFGYERGAFTNASQKKIGRFELAHGGTFF
                     LDEIGELDLGVQAKFLRAIEQETFSRLGGTDEIKVDVRIIAASNRDLEQMAKSAEFRP
                     DLFYRLNVVSLFLPPLRERREDITLLLDHFLRLKAQEHSITIKTLSPEVVDFFMSYPW
                     PGNIRELENLIERLTILTPQEIVTLRDLPPSMRSTDQTATLKEDVLSGSRPLSDAVDE
                     FERELIVKALQRTGFNQTKAAMILNTSRRILKYRIEKLKIINPNDDEEEEVKLSS"
BASE COUNT         1959 a         2390 c         2261 g         1976 t
ORIGIN      
        1 cacagtatcg acgctgctgc cgttctcgca ccagacgatc tgattgacta cattgttcaa
       61 cggtcggtag gccggtgagt cgcggcgcaa aagaacgatg tcggccaact ttcccggcgc
      121 gatgatgccg gcgtctaatc caaatgcgtg ggcgccgttg cgcgtcgcca tcgccagtgc
      181 ctgcgccggc gtgacccacc gattgaaatc gctgcccgcc tgattgtgaa tcagagcggc
      241 caagcgcagc gcgtcgaaca tattctggcc gtcgttagat gccgcgccgt cggtgcccaa
      301 tccaacatgc acgccagcgg caagaaactc ttttatcttt gccaggccgc tgccgatgcg
      361 aagattactc gctggattgt gcaccggcgt ggcgccttgc ttggcgaaaa gctccacatc
      421 gtcgtcttcg atccagatcg aatgcgcgca agaaagattt tcaccgacga ggccaaggga
      481 ttcgagatgc cgtagcagag aatggccgta tatcgaattg ccctgcacct gctgggcttt
      541 ggtttccgcc agatgaatat gcagcggcaa gtttttttta cgcgctattt cagcgcagcc
      601 tttgagcaat tcgtcggagc aacgctgcac ggctgacgga ccgggacaag ccgtggtcag
      661 tttctccggg gcgtgaaacg tctcgatgaa ctcctcacat tcgtcgagcc aagctttcgc
      721 tgtcttcgct tcacttgcgc tcatgcgcat catctcattg ttcagagccg cgtcggtatg
      781 accgagcggg atcgtgtctt cgtagttctt gtcggtcacc gtaagcgccg cgacgtgacg
      841 caaaccgaga tcgcgcatcg cctggatcgc cgccccggcg cccatgaaac ggcaggcagc
      901 gctgccggca aaatgatcga gcaccgtagt cgcgccgttc atgagcatct cgatgccgcc
      961 gagcaacgcg ctggcgtaaa aatcaccctc gcgcatatcg cgcaacgacg cgcggtaata
     1021 ggcgcgccag atttccaacg gcttgcttgg catcatgccg cgcactaaat tcgccggaga
     1081 atgacagtgc gcgttcacca tgccgggcat tgcgatcatg cggctggcat cgatcgtcct
     1141 gtcaaacggt ccggccggcg tccattcggc cggcccgcag taagtgatcc tattgtcgtc
     1201 gacgacgatg gcgcctttct caacaatatc gttgtgatcg ttcatcgtga cgatcagcgc
     1261 gttggcaatc agcgttctca taacgtcctc ttgtgactcg tgaaactttc tcgcaagcaa
     1321 aaaagcaagg gcacggcaag ccgtgccctt gcaaaatcta gaaaagaccg atctgaatgg
     1381 ctagatgcgc gtcccgccat ggccgacgag cgcaatacag ttgggtcgaa tcgaccgcgc
     1441 acaatgcagc gggtcgccct cgacggtcta tacaaacccc atcttgcggc cccagttctc
     1501 aggatctcgc aagaaatcga tgatatgcgt attctgttcg gcggtgaaat agtttttgct
     1561 ggttccggtg tccaagacat catcccagtt ggcgagaaca tgcaaggcaa tgccgttctt
     1621 agccaaagta tcgcggcctt catgttgatc gagtcggtcc gacgcgtagt cgaacacgca
     1681 caggcaatgg gtcattttcg ccccggcacc gcgcacgccg atattgaagc gcagcttact
     1741 caggccatcc gtaattaagt cttcgaccat aagcacgcgc ttgcctggat cgagaatgcc
     1801 ttcgatctgc ttggtgcctc cgtagccttt gggctgcttg cgaatataga tcatcggctt
     1861 tttgacaatg tgcgcgacga acgccgcgta aggaataccg gcggtctcgc ctccggcgac
     1921 gacatcgaaa ttatcgagac caatgtcggt ttcggtcatc ttcgcgagcg ccgtgacgat
     1981 atattcccgc tccgcagcga acgacaacag cttgcggcaa tcgatataaa ccggcgagat
     2041 gcgcccggag acgaagacgt acggtttgtc tttgtaaacc gcgatcgact ccgtcgacag
     2101 cagcatttcg gcaactttct tggacacttc gctcatggga caacctcgca gattttggat
     2161 taggcaaggt cataacatag cgatattcgc tttccaacgc ttgcgcggcg taagcgacgc
     2221 aagcgttgga gcgagaatat gcagcatgaa gtgcgaacgg cagaaaatta ggacaaattg
     2281 tcctacggcg ctttagtgac ttcgtgttgt gaatgatatt ctgcgacagc gacgagctta
     2341 acaaaggcag gaaccgagat gatgctgtcg ctgcgagaat agccggcgct attccgcagc
     2401 tcgggcatag agacgggtca tttcaccttc aacgaaaaac ccttggtctc tttcttagcc
     2461 cttttgtctg cgcgcttttc catcaaggtc ttaagcggct tcttcttccc ttgtttcttc
     2521 gagtccatcc ctttactcat atgcgtggct ccttctgatc tgtttgacgg gcgtagcaga
     2581 aaaccacgca cgtcggtcga taaactccat catccgcaac agcactgcta tttcgcacag
     2641 caattcttga acttcttgcc gctgccgcat aggcacggtt cgttacgtcc aaccttcttc
     2701 tcggcaacgg taggtttctg gggattcaag agccttttca ggtcgctgag atcctcggcc
     2761 tcatccggct cgatgctaat gcggtattcc cagccatgtt tctcgaacgt ctcgcgcact
     2821 tcttttgatc gcgctgccga ctggacccgc accgacgctg gatttttctc gctgcccagt
     2881 ttggcggttt tcttcgcgcc aaaagtattt atccgactat ctgcgtcgac actactacgc
     2941 attatgccga tatcagtgat tcgattgctc atgcatttag tatttcagtt atcgacgcgt
     3001 taatctatca tgtttgacac aagcagcctt tagcgaagtg ccggttggtg cttgtcatcc
     3061 cagttcgagg ataattcgct tactaagggc gcagcttata tctccgctcg atgtcttcga
     3121 catacccact cttcaccacc ttcgtcaata cgcgctgatc gagcagctcc tcgacgttga
     3181 acttaacgat cttcggatcg cgcatggaaa taatatcctg agccgttttc agacccttgg
     3241 gcgtgacgaa ggtgtagctg gtcaagtcgt cgaggagaaa tttgtacccc agctcggcga
     3301 gctggcggtc gaccttaaga tatttttgca acagtgcgat gccgcgctct ttattcgctc
     3361 gcatgaacgc gctggcttcg atcatcgctt tgatgaagtt ctcgatggtc tgcgggtgtt
     3421 tctgcacgaa agtttcggcg cacatgaatg gactagaggg cagtgcgatt ttgctcaagt
     3481 cgagcaacgg tttcacaccc aaagtctgca gccgcgccga ttgcagttta tccaacgccg
     3541 caccgtcgat aatgccgctc gccaaagatt gcgcaatcac cgtgctgtcg ccgatcgctt
     3601 ggatgctgat attgtccctt tgcgggtcga gacccatttc gcgcagtgac agcatggtga
     3661 gactccacag cagaccgctg aagtcctgta caccgatctt tttgccgcgt aagtcgccgg
     3721 gcttggtgac cgccggtgca actaagaaat cgtaggacgc ccgctcggcg aagagtgaca
     3781 tgatgatttt cagcttcgcc ccttgggcgg cggcggaaag catgctcgaa gcgctgccgc
     3841 ggacgaactg gacttcgtta gataccaacg ccgacacctg aatcggtgca ctacgcacgg
     3901 ccaccaagtc cgcttccaag ccatgcttcg cgaaaaggcc ttcctcttta gcaagccaga
     3961 ccggcgtacc ggaggcagac accccggaat aagcgatgcg aatcggctcg gcacacaccg
     4021 gtgcgcccca gcctatcacc agagcgatca gcaagagtct tatagcaccc ataaggaaaa
     4081 tccccctata cctgagtgag tagatgtgac gaggtgttgc tattggaccg cttctaacat
     4141 gacaccagtg cggctgacaa tgacggttgc cttacgcggc acccagatga aataccaata
     4201 ctaagcgaca gcggaagctg ctaaactaat tccgaatgaa gcgcccaccc acggccgtac
     4261 gtccgtccag cctaccagcc atcaatcagc gccgtctcat gcgcgactta aattccatcg
     4321 gccgcattgg tattggcgac cacggcggtg tgacacgcct ggtgttttcg atcaaagaat
     4381 tgcgcagccg gcaggtgctg atccatctta tgcgccaggc cggactgaag atccacatcg
     4441 atgccatcgg gaatatcttc ggccggctcg agggccacag tcccaaggcg ccggcggtac
     4501 tgtcgggatc gcacctcgat acggttattc atggcggcaa gtacgatggg cctgtgggtg
     4561 tcattggcgc cctggaagcc gtgcgcacaa tcaacgagaa ccgcgttcca atccattcgc
     4621 cgctcgaagt cgtttgcttc gtgggtgagg aatcgagccg ctttggcttt tccactctgg
     4681 gcagcagccg ggttgccggc gaagttcatg caaaagatct cgccaatgcc gttgatgccc
     4741 aggggacaaa gttagccgac gtgttggcga gcctcggcat agcccgtaaa aatctcggca
     4801 gcctcgcccg taatccgaaa agcctcaagg gctatctgga actccacatc gaacaagggc
     4861 cgatcctcga agcgctcggc aaaagaatcg gcctggtcac ctccatcgcc gcaccgaccc
     4921 gattcaaggt gatcttcacc ggacaagcgg atcactccgg caccacgccc atggaaatgc
     4981 gcaaagacgc attggtcgcc gccgcacggt tgatcgaatt cgtcgaaaaa acctgccgcc
     5041 gattttccgc gatggccaaa ggccgggtcg tcggcaccgt cggcgcctta aaggtcgagc
     5101 caggcgtcat taacgcggtg ccggggaaaa ccgaactgtt ggtggatatt cgcgccatca
     5161 cggcacaggc caaagatcgc gtcgtgcgcg aagtcagggc ggaagcgcaa cgcatcgcgc
     5221 accggcgcgc tattgctgtc gagattctca ccatacgtga agaagaccct gtgcccctga
     5281 accatcggtt tttgcaactg accaaaaaaa tttgcgctga gaaaaacatc gtctatgaaa
     5341 tcatgccatc gggcgccggc cacgatgcca tgcaaatggc caaaattaca ccggcggcaa
     5401 tgatttttat tcccagccaa cgcggcatca gccacaatcc actcgaatgg accgacgccg
     5461 aggacatagg attgggcgcg caaatattaa tggaaactat ggtcagagtc gctaatgaaa
     5521 aattttaaag ccaagaagaa gcgaattctc accgctgtcc agattgcagc gatttttgag
     5581 cgcttcgcga gcgagatggc taaaacctat gagagcctgg agcatgtcgt tttcatcggc
     5641 gtccgaacgc gcggccccta tgtcgccaaa cgactcgctg cgatgatcaa gaagcgccat
     5701 aagaacgaga tacccgtcgg cgagatggat atcacactat accgcgacga cttgaatgcg
     5761 ctcctgcccg gcggcactat cgatcacacg cgcataccgt tcgacatagg caacaaaatc
     5821 gtcgtgttgt tcgatgacgt gctgaacact gggcgaaccg cgcgcgccgc cgtcgatcac
     5881 ctgatcgatc tcggccggcc gcgcatgatt cacctggcgg tgataattga ccgcggcggc
     5941 cgcgaactgc cgatcgctgc caactatgtc ggcaaaacga tccgtttaaa aagcggcggc
     6001 aatgtcgaag tccggctcaa ggaagtcgac aaagtcgacg aagttttgtt gggataaagc
     6061 acccgcgatt aaggttgcgc cccgcgcttg gcagggcacc gaccataaaa agaccaacca
     6121 cttgtttatt gaaataaacg tttgtcattt tgtgttgtga cagacacttt ggctgcctag
     6181 gacgacacat gcaggggcta gacggacgag atcgcgtctg attcggcagg ggaacggaat
     6241 tagcggatta ggttgtaaga tttctgaccc gaaactgaca agatatagag caagatctaa
     6301 tacctgatat aaagcaacta tgattagcct cacagaaaat ttgttgttat gcatattttc
     6361 gctgctcgac ggctaaggga gcagcaacaa caacttgtgc cgggaagctc atgaatactt
     6421 gcgtgcccgc attcggctgg ctggtaacac tgagtttgcc gccgacgaga tcggccatgc
     6481 gacgtgctac ggttagcccg atacccaggc ctgagaaggc tctttgtgtc gaattgtccg
     6541 actggtgaaa aggttcgaag atcgtatcga tcttgttctg ctcaatacca actccggtgt
     6601 cgcgaacttc aaaatccata tgggtgcgat ccggcgacgt gtcgactttg accgtaatct
     6661 ggccctgagc ggtgaactta acagcattgt taaaaacgtt ttgaaacacc cgttcgactt
     6721 tggcgcggtc attgaccatc gacaaatcgc catcagagaa ttgaacgtcc agctgcaatc
     6781 ctttgtcgcg gatcatcttt tcgtgccgct tgacagcttc tttgacaacc tcgcgggcat
     6841 cgaacttctc cacgatcgcg ctagtatcgc cggcgtttag tgatgtcatc cacagcacgt
     6901 tatcgatcag ttccagcagc tcttccgagt tgcgatagac ggtctcgaga actttttgct
     6961 gctcttcgct gagcttacca atgacctggt ttaaaagtag ataaacgaac ccgacaacta
     7021 cactaagcgg cgtgcgcaat tcgtgagaaa tattggagag aaagtcggac ttgacctgac
     7081 gcagcttggc cttggcggtg cgtcgttcta acccccgccg cacaagagaa agtatatgat
     7141 tgacatcgaa cggtttggat atgtagtcga acgctcctag gcgtaagcct tcgattgcag
     7201 tgtccagaga gccatagccc gtgatgatga tcgcttctat gtcaggatca tgggctttga
     7261 ccttctccaa cacattgatg ccgctcagac caggcatctt gagatccagt gtcaccagat
     7321 ccaccgagtg ctccttgagc atctcgatag cctgcgcccc gcgatcggct accagaacgt
     7381 tgtagtaggg attgagaatc attttgagcg attctctggg ccccatctca tcatcgacaa
     7441 cgagaatgtt gggtttacta gcatccatgc tgatgtaatt tcagcaaggc ttatgccagt
     7501 gaggcaattt agtaacgcac tgaatttata agaagttttt cggcaatgaa ggagcgctaa
     7561 ggaagctgag ggaaacaaaa atgtccaact atgacgataa tttcacttcc tcctcttcat
     7621 cgtcgttcgg attgataatt ttaagctttt cgatccggta tttgagaatg cgccggctgg
     7681 tatttagaat catcgccgcc ttggtctggt tgaaaccggt gcgctgcagg gctttgacaa
     7741 tcagctcgcg ttcgaactca tccacggcgt cacttagggg acgcgatccg ctaaggacat
     7801 cttcctttag cgtcgcggtt tggtccgttg aacgcatgct cggcggcaaa tcgcgcagtg
     7861 taacgatttc ctgcggcgtg agaatggtca accgttcgat taagttctcc agctcgcgaa
     7921 tattgccggg ccaagggtaa gacatgaaaa agtccactac ttccggtgac agcgttttga
     7981 tcgtaatcga atgttcctgg gcttttaggc gcagaaaatg gtcgagcaat aatgttatgt
     8041 cttcacggcg ctcacgcagc ggcggcaaga ataaagaaac cacgttcaaa cggtagaata
     8101 aatccggtcg aaactcggcg ctcttggcca tctgctcgag atcgcgattg ctcgcggcga
     8161 taatgcgcac gtccactttg atctcatctg tgccgccgag ccggctgaag gtctcttgct
     8221 cgatggcgcg cagaaatttc gcttgtacgc cgagatcgag ttcgccgatt tcgtcgagaa
     8281 aaaaagttcc gccgtgggcc agctcgaaac ggccgatctt tttctgcgag gcattggtga
     8341 atgcgccccg ttcataccca aacagctcgc tctccatcaa agtttcgggt aacgccgcac
     8401 aatttaccac cacgaagggt ttggaacgcc ggtcgctgtt gtaatgaatc gccctggcga
     8461 tgagttcttt accggtgccg ctttcaccgg taatcagcac cgtgcttttc ttggccgcca
     8521 ccatactgac agccttgaag acatcggtga tttgcctcga cttgcctata atgaaatcga
     8581 tcccat
//
LOCUS       DATLCH010000181         4711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5707887, whole genome shotgun sequence.
ACCESSION   DATLCH010000181 DATLCH010000000
VERSION     DATLCH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5707887"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VMZ02_05355"
     CDS             <1..309
                     /locus_tag="VMZ02_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HUR71391.1"
                     /translation="GDAVSMPENYRWGWSYIPHFIHSRFYCYSYVFGQLLVLALYRMY
                     KDEGKSFVAKYIALLEAGGSDSPDALLKPLGVDIHQPEFWQKGFEEISGLTAKLNALI
                     "
     gene            complement(435..1877)
                     /locus_tag="VMZ02_05360"
     CDS             complement(435..1877)
                     /locus_tag="VMZ02_05360"
                     /inference="COORDINATES: protein motif:HMM:NF004801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase family protein"
                     /protein_id="HUR71392.1"
                     /translation="MKTRIKGGWVVGFDGKEHEIIKDGVVVYDGKRIEFVGHEYNQSV
                     DKEIDATGCLVSPGFIDTHFHSGINASDYILADSSKTDFFASNYLAHGGPTREGAHAA
                     HLKDVDIGQRFSIIHVLKSGVTTTFDIGGAGGDPARYVDMIDEVGIRCVAGPSYKNVS
                     FFHDKEGRLEYDWNDERGSKGLQSAVEFAKNYNGKCSGRMTAMLFPGHVDSCTPAILT
                     ETKKAAKDAGVGIQIHATINLFEFHTVMQRERCTPIELLQKIGFLSPDVSLSHCIFVS
                     GHSWLAYPYGDDLKIIADSGASVAYSPLKYLKLGILMESFDRYRQAGINMGIGTDTFP
                     KDILSDMRYAAISSRVADKSFLAGHPRDVFNSITLGGAKMIGRDDIGRLAKGAKADIA
                     IVNLRDLAFGAVHDPIRSLMETAVSRDVRTVIVDGETLVDKGKFLRLDESELLDKVQA
                     KGEQVWDSVSKWHWTGKGIDEVVPPSFRMK"
     gene            complement(2116..3180)
                     /locus_tag="VMZ02_05365"
     CDS             complement(2116..3180)
                     /locus_tag="VMZ02_05365"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71393.1"
                     /translation="MGQRFSVKLLNRHVAVHELLIKTWTLLVLSVLSSSVAIAQVNPY
                     LARPGERPMSIRIATCAVSGGFAHLYTALENNLFDKYGFKIEHVFIRGSGPALAALMA
                     DEIQFLYCAADATLPSLAAGVDVKLVAAPLVKLPYVLVTRREIRRMEDLKGKALGVAR
                     AGDLSDRLSRAMVKRFNVTDVTMRPIGGSQNERYQAMVANLVQGVVVTPPIDVRAKND
                     GYHVLYRLIEMDIPFIYSSLHASSRMVRDRPEMVQRMVAAFAEAIHYVDKNPGKTKSA
                     IAKIMRTKDEEALQVSYNVYTKEIVDRRMIVPGQAVNDSLDLYRALGTPVKRRAEDIY
                     DNSFVNNLERSGFQRELWGR"
     gene            complement(3455..>4711)
                     /locus_tag="VMZ02_05370"
     CDS             complement(3455..>4711)
                     /locus_tag="VMZ02_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUR71394.1"
                     /translation="NVSTTTVRAASERSAPGPFGGTDPGEEFWQRFFGGRTPRGPQRQ
                     SGVGSGFIVDADGTILTNYHVVDGAQKLSVTLSDGKSYDAKIIGRDQKSDIAVIKIEA
                     GVKLPAAVLGDSDLLEVGEWVMAIGNPFGLDHTVTSGIISAKGRNIGQGPYDNFIQTD
                     ASINPGNSGGPLINLRGEVVGINSAIFSQSGGNIGIGFAIPSNAVKELLPQLRDKGKV
                     VRGFIGASVQRVSPEIAESFGINPARGALVADLVKGGPAERAGVKTGDVIIEFDRKPI
                     KDSADLPQQVARVAPGSAVQLKLIRGGKETTLPLTVGEMKEAEVAASTEEGELGLTVQ
                     PLTPQLAESLGLERANGLVISAVKPGSAADDAGLRAGDVIMEINRQAVKSLAEYNREI
                     ARNDKAKSVLFLVRRGQSSLFLALKR"
BASE COUNT         1042 a         1335 c         1280 g         1054 t
ORIGIN      
        1 ggcgatgcgg ttagtatgcc cgaaaactac cgctggggtt ggagctacat cccccacttc
       61 atccattcgc ggttctattg ctacagctat gtcttcggcc aactcttggt cctggcgctc
      121 tatcgaatgt acaaagacga aggcaaaagc ttcgtcgcga aatacatcgc gctcctcgaa
      181 gccggcggct ccgactcgcc ggacgcccta ctcaaaccac tcggtgtgga tattcaccag
      241 cctgagtttt ggcagaaggg cttcgaggag attagcggac tgaccgctaa actgaatgcg
      301 ctgatctaat cagcggcaga aactgatttc ctacgacaat tcgcaagtag gttgtcgacc
      361 gttcttaatg ggctgccgga aaatactcga ctaatactct ccaatggctt tcggtcaagc
      421 cgccttgcgt ccttttattt catccgaaaa ctcggtggca ccacttcgtc aatacctttt
      481 cccgtccagt gccacttcga cacactgtcc cagacctgtt cacctttggc ttggaccttg
      541 tcgagcagtt cgctttcgtc gaggcgcaga aatttgcctt tgtcgaccaa ggtttcaccg
      601 tcgacgatca ctgtgcgcac atcgcggctg acggcggttt ccattaacga gcggatggga
      661 tcgtgcaccg cgccaaaagc gagatcgcgc aaattgacga tggcaatatc ggctttggca
      721 cctttcgcca gacggccgat atcgtcgcgg ccgatcatct tggcgccgcc gagggtaatc
      781 gaattgaaga catcgcgcgg atggccggcg agaaaactct tgtcggcaac ccgcgatgag
      841 atggcggcat agcgcatatc cgagagaata tcttttggaa aagtatcggt gccgatgccc
      901 atgttgatac cggcttggcg atagcggtcg aaggactcca tcagaatgcc gagcttgaga
      961 tattttaacg ggctgtaagc caccgacgcg ccggagtcgg cgatgatttt taggtcgtcg
     1021 ccatagggat aagcgagcca gctatgaccg ctgacaaaga tgcagtgcga caaagataca
     1081 tccggcgaga gaaatccgat tttttgcaaa agctcgatgg gcgtgcagcg ttcacgctgc
     1141 atgactgtgt ggaattcgaa caaattgatg gtcgcgtgaa tctgaattcc gacgccggca
     1201 tccttggcag ccttcttcgt ctcggtcaat atggccggcg tacaggaatc gacatggccg
     1261 ggaaataaca tcgcggtcat ccgtccgctg cacttgccgt tatagttctt ggcgaactcg
     1321 acagcggatt ggaggccctt actaccgcgc tcgtcgttcc aatcgtattc cagccggccc
     1381 tctttatcgt gaaaaaaaga gacgttcttg tagcttggcc cggcaacaca gcgaatgccg
     1441 acttcatcga tcatgtcgac gtaacgcgcc ggatcgccgc cggcgccgcc aatgtcgaac
     1501 gtcgttgtca cgccgctttt gagcacgtgg attatcgaga agcgctggcc gatatcgacg
     1561 tcctttaaat gcgccgcgtg cgcgccttcg cgggtcggtc cgccatgagc aagatagtta
     1621 gaggcgaaga aatcggtctt gctcgagtcc gcgagaatgt agtcgctggc attgataccg
     1681 gagtgaaaat gcgtgtcgat gaagcccggc gagaccaagc aacccgtcgc atcgatctcc
     1741 ttatcgactg attggttata ctcgtggccg acgaattcga tgcgcttgcc gtcgtagaca
     1801 accacaccgt ctttgatgat ctcatgttct ttgccgtcga agccgacgac ccagccgcct
     1861 ttaatacgag ttttcatggc cgcgcctttc agaaagtgga atgcaaatca gtcgcaggac
     1921 taacaaaacc aaattactaa gacaaggtgc gactacgatg cagccggatt attagacagg
     1981 aagagaaaat ttaatgcaga tgtcgttttg cggatcggcc ggacagggtc agctcgacgg
     2041 acttacgggt gtcgctcgcc accgcgctat gccggtcggt gggtcggtgc tgtcgatggt
     2101 gtatacgtaa ctgactcagc ggccccagag ctcccgttga aaaccgctgc gctccaaatt
     2161 gttgacaaag ctgttgtcgt aaatatcttc ggctctgcgt ttaaccggtg ttcccaaagc
     2221 acgatataga tcgagcgaat cgttcacagc ttgaccaggc actatcatgc gccggtcgac
     2281 aatttccttg gtgtaaacat tataggaaac ctgcagcgct tcttcgtcct tcgtgcgcat
     2341 gattttcgcg atggccgatt tggttttccc cggatttttg tcgacataat gaatggcttc
     2401 ggcgaaggcg gcaaccatgc gctgaaccat ttcgggacgg tcgcgcacca tccgcgagct
     2461 cgcgtgaagg gaactgtaaa taaacggtat atccatctca atgagacggt agaggacatg
     2521 ataaccatcg ttcttagcac gcacgtcgat cggcggggtg acgactacac cttgcaccag
     2581 gttggcgacc atagcttggt aacgctcgtt ttgactgcca ccaatcgggc gcatggtcac
     2641 gtccgtaaca ttgaaacgtt taaccatggc gcgggataag cgatcgctca gatctcccgc
     2701 ccgtgccacg cccagcgctt tacccttgag atcctccatg cggcggatct ctctacgcgt
     2761 taccaagaca tacggcagtt ttaccagcgg cgcggcgact aacttaacgt cgacgccggc
     2821 ggcgagcgat ggcaaggtcg catcggcagc gcaatagaga aattggatct cgtcggccat
     2881 caaagcagcc agtgccgggc cgctgccgcg gatgaagaca tgctcgattt tgaaaccgta
     2941 cttatcaaaa agattgtttt ccaaagccgt atagagatgc gcgaatccgc cggagacggc
     3001 gcaggtggcg atgcgaatgc tcatggggcg ctcgccgggt cgagcaagat agggatttac
     3061 ttgggcgatc gcgacgctgc tagaaagaac gctcagcacg agcagcgtcc atgtcttaat
     3121 taagagttcg tgcacagcga catgccgatt gagaagtttg accgaaaatc gttgacccat
     3181 tagttacctc gcgtcgcgct taagccggtt acccgcgcgg atattagacg attcctccag
     3241 gcggctcaat gcacggcaag gcgaattgct ggcagggttt cgccggctcc ggcactttcc
     3301 ggcgcaagct aatccgcgcc cggcaagtcc gcgccaagtg gatcgcgaat atctgtagct
     3361 gcttaatgaa acgatgggaa aagctgaaac cgctcacttg cccggaggct gcttgggagg
     3421 catggcaacc tgagcggtct caacccgttg ctggtcagcg ttttaaagcg aggaacaaac
     3481 tactctgacc gcgccggacc aaaaacaaca cggatttggc tttatcattg cgggcgattt
     3541 cccggttgta ctcggcaaga cttttcactg cctggcgatt gatctccatg ataacatcac
     3601 cggcgcgcaa acccgcgtca tcggcggcgc taccgggctt tactgccgag ataaccagtc
     3661 cattggcgcg ctccaaaccg agactctcgg ctagttgcgg tgttagaggt tggacagtca
     3721 gtccgagttc gccctcttcc gtcgacgcag cgacctcggc ctccttcatt tcgcctacgg
     3781 tgagcggcaa agttgtttcc ttgccgccgc gaatcaattt caactgcacc gctgaacctg
     3841 gcgccacccg cgccacctgt tgcggcaggt cggcggaatc tttaattggc ttgcggtcga
     3901 attcgataat aacgtcgccg gtctttacac cagcgcgctc ggccgggccg cccttgacca
     3961 gatcagcgac caaggctcca cgggccgggt tgatgccaaa ggattcagcg atttccggac
     4021 taactctttg caccgatgcg ccgataaacc cgcgaacgac tttgccctta tcgcgcaatt
     4081 gcggcaacaa ctccttcact gcattcgacg gaatcgcgaa gccgataccg atattgccgc
     4141 cggactggct gaagatcgcg gaattaatac caaccacctc gccgcgtaga ttgatcagcg
     4201 gcccgcccga gttgccggga tttatcgagg cgtccgtctg aatgaagtta tcatatggcc
     4261 cttggccgat attacgcccc ttagcgctga tgattccgga agtaaccgtg tggtcgaggc
     4321 cgaacggatt gccaatcgcc atgacccatt cgcccacttc gagcagatcg gagtcaccca
     4381 acactgccgc cggtaacttc acgccagctt cgatttttat caccgcaatg tcgctctttt
     4441 gatctctgcc gattattttc gcatcatagc tcttgccgtc ggacaatgtg acggaaagtt
     4501 tttgtgcccc gtcaacgaca tgatagttgg tcagaatggt accatcggca tcgacgatga
     4561 accccgaacc gacaccgctc tggcgttgcg gcccgcgcgg cgtacgcccg ccgaagaatc
     4621 gttgccagaa ctcctctccg gggtcggtac ccccgaacgg accgggcgcg ctccgctcgg
     4681 aggcggcgcg cactgtagtg gtcgaaacat t
//
LOCUS       DATLCH010000182         4134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4965963, whole genome shotgun sequence.
ACCESSION   DATLCH010000182 DATLCH010000000
VERSION     DATLCH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4965963"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VMZ02_05375"
     CDS             <1..1403
                     /locus_tag="VMZ02_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HUR71395.1"
                     /translation="FGGNAEDVSVTASVVHDHGRVYEFISRGLTFFTSEVWLRRGDEW
                     VYIDKPNDARIETFTDQILLRLRSDWTVNGKTYRAGSLLAAELEAYLRGEREFVVLFE
                     PSERKALTSTSATKNYLILNELDNVRSRAYALKRDSGQWTRSELTTPEFGSVRVRGVD
                     AAESDDYFMTATDFLTPSTLCLGTVGVATQEKLKGLPEFFRADDLTISQHEAVSKDGT
                     RIPYFEVSRKELTTNGHNPTLLYGYGGFNVSQLPFYSGTLGAAWLERGGVYVLANIRG
                     GGEFGPNWHYAARKENRQRAYDDFIAIAEDLIGRKVTSPQHLGIQGGSNGGLLVGVAL
                     TQRPDLFKAVVCQVPLLDMQRYNKLLAGASWVDEYGDPGKPEEWAYIEKYSPYHNVFA
                     DKKYPRVLFTTSTRDDRVHPGHARKMFAKMKAQGHDVVYYENIEGGHGGAADNRQLAY
                     MSALTYSFLVKELFAD"
     gene            complement(1497..2588)
                     /locus_tag="VMZ02_05380"
     CDS             complement(1497..2588)
                     /locus_tag="VMZ02_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71396.1"
                     /translation="MKLGLFDHMQKHDNPARSYADLYRNHLEVLEFADQAGMDFYFVA
                     EHHFDMGFSECPSPGSFLGAASQRTKNIRMGPLVYVLPLWHPVRVAEETALLDNLMNG
                     RLECGIGAGIGPFSFKAYNVDWDSKREMTLEALQIIKGIWTHETFSFDGKYFKCKDVD
                     SSIPLVQKPHPPLWMPTRSKESIEEAAATGISTVQWVPSGMKATRKAFDDYRDAYQRA
                     KPAGRKPHMGLMREIYVAESDKQARADGEYHWKNFWERRGGARTYGAHGTTGLTTILD
                     GSRRQQLMDMEHSIAEGSFICGSPETVVQQIRSIANQAGADTFLGEFTFGELTQKQAL
                     NSLRLFTEGVMPELRKFEVDALNFPKSEA"
     gene            complement(2598..3581)
                     /locus_tag="VMZ02_05385"
     CDS             complement(2598..3581)
                     /locus_tag="VMZ02_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71397.1"
                     /translation="MKATGKLLIFSAVLLCSSGLRPQAGAQTAKLRVAYTAFAGTFTV
                     LWVGKDAGLYQKFGADMELLYIGSSTKSVQALLGGDIDIMYSAAGAVVDANLAGAELA
                     MIGCQYDKGQTSFFTTAPVTSIGDLKGKAVGVTRFGAFSDFVARHVLKKHRLQPIKDV
                     ALLQLGGTQEIIAGMQNNLIQGGSVSLPLTINARKLGFRELLTTEAIGLPFDYGCIIV
                     KNSQLKSRREELKRLLRATIAAYDLAIADPAFAKRIIGKYTRTTDTATLEETYKENVK
                     DYALRVPYVSLAGLNSILEFRAESSAEVKKLALDKMYDNSLLQEIQKETTK"
     gene            3760..>4134
                     /locus_tag="VMZ02_05390"
     CDS             3760..>4134
                     /locus_tag="VMZ02_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71398.1"
                     /translation="MANSNRCILSMLIAMAVYCLAPSRAIGEEKIKFPIGVGTKTVGT
                     HMFWLAAKKGFFEEFGLDVQPVWLRGTSITIQALVSESLYLALGSADSTIGNAPSTDL
                     IAVSGVVGGLTQAIVAAKRYKSI"
BASE COUNT          878 a         1079 c         1200 g          977 t
ORIGIN      
        1 tgtttggagg caacgccgag gacgttagcg ttactgcttc ggtggtgcat gaccatgggc
       61 gcgtttatga gttcatttcg cgcggcctga catttttcac cagcgaagtg tggcttcgcc
      121 gcggcgatga atgggtctat atcgataaac ccaacgatgc acgtatcgag acgttcacgg
      181 atcagattct tttacgcttg cgttccgact ggacagttaa cggcaagact taccgcgcgg
      241 gctcgttgct cgctgccgaa ttggaagcgt atttacgcgg cgagcgggag ttcgttgtgc
      301 ttttcgagcc gagcgagcgc aaagcattga cgagcacgag cgcgaccaaa aactacttga
      361 tccttaacga actggataat gtgcgcagta gagcttatgc gcttaaacgc gacagcggcc
      421 aatggacgcg cagtgagctg acaacgcccg agttcggttc ggtacgtgtt cgcggcgtcg
      481 atgccgctga gtctgacgat tactttatga cagccaccga ctttctcacg ccttcgactc
      541 tttgtctcgg tacggtcgga gtggcgacgc aggagaaatt gaagggacta ccggaatttt
      601 tccgcgccga cgatttgacg atcagccagc acgaagcggt gtcgaaagat ggaacgagaa
      661 ttccttactt tgaagtgagc cgaaaagaat tgacgacaaa cggtcataat cccacgctgc
      721 tctatggtta cggtggcttc aatgtttcgc aattgccgtt ttacagcggc actctggggg
      781 cggcttggct ggagcgcggc ggtgtctatg tgctggcgaa cattcgcggc ggaggtgagt
      841 tcggtccgaa ctggcactac gccgcgcgca aggaaaaccg tcaacgcgcc tacgatgatt
      901 ttattgcgat cgccgaggac ttgatcggac gcaaagtgac ttcgccgcag catctcggca
      961 tccagggcgg ttcaaacgga ggattactgg tcggagtcgc attgactcag cggcctgatc
     1021 tattcaaagc cgtagtctgc caggtcccgc tgctcgatat gcagcgttat aacaagctac
     1081 tggccggcgc gagttgggtc gatgaatacg gcgatccggg caagccggaa gagtgggctt
     1141 acatcgagaa gtattcaccg tatcataatg tcttcgccga taaaaaatat ccgcgggtgc
     1201 tgttcacgac ttcgacacgc gacgaccgcg tgcatccagg ccacgcgcgc aaaatgttcg
     1261 ccaagatgaa agcgcaaggc catgacgtgg tttactacga aaacatcgaa ggcggccatg
     1321 gcggcgcagc ggacaatcgg cagctcgcct atatgtcggc gctaacctat tcgtttctgg
     1381 tcaaagagtt gttcgctgat tgaatggaat cgctgcgtgc taccgcaaaa ttgaagacgc
     1441 tcaccgtacc ctgccacaag tgcgtgaggc agctacattc tcgaaagttt ttgcccctat
     1501 gcttccgact ttggaaaatt cagggcgtcg acttcaaact ttctcagctc cggcataacc
     1561 ccctcagtga ataagcgcag cgaattaagc gcttgcttct gcgtcagttc gccgaacgta
     1621 aactcgccga gaaaggtgtc ggcgccggct tggttggcga tcgatctaat ttgctgcacc
     1681 acggtttccg gcgagccgca gatgaacgag ccttcggcga tggagtgttc catatccatt
     1741 aactgttggc gccggctacc gtcgagaatc gtcgtcaacc cggttgtgcc gtgggcgccg
     1801 taggtgcgcg cgccgccgcg ccgttcccag aaattcttcc aatgatattc gccatcggcg
     1861 cgggcttgct tatcgctctc agcgacatag atctcgcgca tcagtcccat gtgtggtttg
     1921 cggccagccg gtttggcgcg ctgataagcg tcacggtagt catcgaaagc tttgcgcgtc
     1981 gccttcattc cggagggcac ccattgaacg gtgctgatcc cagtggcggc ggcttcttca
     2041 atggactctt tgctgcgcgt cggcatccac agcggcggat gcggtttctg cactaacggg
     2101 atgctggaat cgacatcctt acacttaaaa tattttccgt cgaaagaaaa agtttcatgg
     2161 gtccagatgc ctttgatgat ctgcaacgct tccaacgtca tctcgcgttt cgaatcccag
     2221 tcgacgttgt aagccttgaa ggagaacgga ccgatgccgg cgccaatgcc gcattccaac
     2281 cggccgttca tgagattgtc gagcagtgct gtttcttcgg cgactcgcac ggggtgccag
     2341 agcggcaaca cgtagacgag cgggcccatg cgaatattct tggttcgctg cgacgctgcg
     2401 ccgaggaaac ttcccggact gggacattct gagaaaccca tgtcgaagtg gtgttcggca
     2461 acgaaataga aatccatgcc ggcctgatcg gcgaattcca gaacctccag atgattgcga
     2521 tagagatcgg cgtagctgcg cgccgggtta tcatgtttct gcatgtggtc gaatagaccg
     2581 agtttcatgg tgtttcctta tttggtggtt tccttttgaa tttcttgcag cagtgagttg
     2641 tcgtacatct tgtccaaggc gagcttctta acttcggcgc tgctttcggc gcggaattcc
     2701 aagatcgaat ttagcccggc aagggagaca tagggaactc tcagtgcgta gtctttgacg
     2761 ttctctttgt aggtttcctc caaggtagcg gtgtcggtgg tgcgcgtgta cttgccgatg
     2821 atgcgtttgg cgaatgccgg atcggcgatg gctagatcgt aggcggctat tgtggcgcgc
     2881 agaagccttt tcaactcttc ccgcctggat ttgagctgcg agtttttgac gatgatgcaa
     2941 ccgtagtcga aaggcagacc gatagcttcg gtggtgagca actctcgaaa gcctaacttt
     3001 cgcgcattga tcgtcagtgg cagcgagacc gaaccgccct ggattagatt gttctgcatg
     3061 ccggcgatga tttcctgagt accgccgagc tggagcaagg caacgtcttt gatcggttgc
     3121 agccggtgtt ttttcaaaac atgacgcgcg acaaaatcgg agaaggcgcc gaagcgggtg
     3181 acgccgacgg ccttaccttt caagtcgccg atgctggtga cgggagcagt ggtgaagaat
     3241 gacgtttgtc ctttgtcgta ttggcagccg atcatcgcca gctcggcgcc ggcgagattt
     3301 gcatcgacca ccgcgccggc ggccgagtac atgatgtcga tgtcgccgcc gagcagtgct
     3361 tggactgatt tagtgcttga gccgatgtag agcagctcca tgtcggcgcc aaacttctgg
     3421 taaagtccgg catctttacc gacccacagg accgtaaagg tgccggcgaa tgcggtgtag
     3481 gcgacgcgga gcttggccgt ctgcgcgccg gcctgcggac ggagacctga cgagcacaac
     3541 aaaaccgcag agaaaatcaa tagttttcca gtggctttca tcgccgctcc tgagaatctt
     3601 gcgagcggtt tcgatagatc atgatcgcga cgttgctgtc aagttttcgc cggcgatgtt
     3661 gcgcgacctt aaatcgcctt gaccagcctc gcgtttgtaa attatggtga atcgtcctaa
     3721 cagctcaaat taattagctc cgcagcaatt ttattctcaa tggctaactc gaatcgctgc
     3781 atcctatcaa tgctcatcgc catggcggtt tactgccttg cgccatcgag agcgatcgga
     3841 gaagagaaaa tcaagtttcc cataggcgtg ggcaccaaga ccgtgggcac gcatatgttt
     3901 tggttggcag cgaaaaaagg tttcttcgag gagttcgggc tcgatgtgca accggtctgg
     3961 ttgcgcggga cgtcgatcac gattcaagcg ttggtcagcg aatcgcttta tctggctctc
     4021 ggttcggcgg attcgaccat cggcaatgcg cccagcactg atttgatcgc ggtaagcggc
     4081 gtggttggcg ggctcaccca agcgattgtc gcggcgaagc gctacaaatc aatt
//
LOCUS       DATLCH010000183         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5713572, whole genome shotgun sequence.
ACCESSION   DATLCH010000183 DATLCH010000000
VERSION     DATLCH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5713572"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VMZ02_05395"
     CDS             complement(<1..307)
                     /locus_tag="VMZ02_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUR71399.1"
                     /translation="MLQSLSQKSSKDSQPGELSLVIAPTEVADVMVGKVATLSTHHGF
                     DEAVRLMNDRHFRHCVVVDAHGKLVGVISDRDILRSLARHANSRAKSLDQIMTPNPIT
                     "
     gene            639..1700
                     /locus_tag="VMZ02_05400"
     CDS             639..1700
                     /locus_tag="VMZ02_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71400.1"
                     /translation="MTLILNNRDVGEALNVGDCLKVMEESYREQAGSRAVNRPTCHSY
                     LPHSLPNSTYSFKSVDGGVGKYGVLALRITSDVVQEQEVHGTVRLEKIPLAGTGLFVG
                     LVQLYSSETGELLAIMPDGLIQQTRVGVTSALGMKVMARENSQILGLIGSGGQAKAHY
                     RYMTAVLPIKKVKVFSPNAEHRNAFVAEMEKETGVAGESVASPEEAVRGCDVICSATN
                     SSRPVIKIDWLESGMHYNSIREFETDLEVLNRCDPIAIHTQFGGIQHYQPPGIVDDMP
                     GVRREKPRDWTKYPEICDLIAGKLPSRTSDKQISFFLNNVGTGVQFAAMGYCAYKAAK
                     ENGMGREIPSEWFMQDIKP"
     gene            complement(1711..2745)
                     /locus_tag="VMZ02_05405"
     CDS             complement(1711..2745)
                     /locus_tag="VMZ02_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71401.1"
                     /translation="MRLKLVVICLAFNLMGVLPSHGQTPNFQGKTMTIVVGTVAGDLY
                     DLYARAIALFMGKYLPGNPNIIVQNMPGAGHMIAANYVYNVAKPDGLTLGAINAGLYF
                     EQLIGRAEVKFDWTKFNWIGNATKSPQVLYMRADAPFKSIEDVRNAKEAPKCGTTGMS
                     NMGYFVPKLLEETVGAKFNVIAGYQGGNEIDLGVERGEIVCRSLSTEAYFSREPFLTW
                     RKNGFSRELTQGGKVRLEKLANVPTVYELMDKYKTPDTGRRLATALLASGEFHRPYMA
                     PPKMRAEIVKALRDAFTKTMKDPDFLAETKRKRLDMDPTTGEEVQALAADVMTQPKDV
                     VDKLKNMMGR"
     gene            complement(2810..>3109)
                     /locus_tag="VMZ02_05410"
     CDS             complement(2810..>3109)
                     /locus_tag="VMZ02_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71402.1"
                     /translation="ELMDQFKTTETNRRIANVLLGGAEFGRFMLVAPGTPPDRVKTLR
                     DAYGRSMKDPELIAEAKKGRMDMDPTSGEELQTLVNEIMTQPPDVIERVKKILSE"
BASE COUNT          702 a          847 c          832 g          728 t
ORIGIN      
        1 cggtgatcgg attaggcgtc atgatttgat cgagcgactt cgctcgcgaa ttggcatggc
       61 gcgctaaact gcgcaggatg tctcgatcgg aaataacccc caccagctta ccgtgagcat
      121 cgacgacaac gcagtggcga aagtggcgat cgttcatcag acgcactgct tcgtcgaagc
      181 cgtgatgcgt gctcagcgtt gcgactttgc cgaccatcac gtcagccact tcagtgggag
      241 cgataactaa cgacagctca ccgggctggg agtctttcga cgatttttga ctcaagcttt
      301 gaagcatggg caccctctca atcgagctta gaaacctgtc ggtttacgcg aacataaatt
      361 tagcaattct cacaccagat tctctgcaag acttgccgag tgggccgata gacacgagct
      421 ctcccgcgat tacacaaacg cttcgaaaaa gggagtcgaa ggtaaacaag ttccatcaaa
      481 gttccgacca agtctgtccg aatcgttcca aagatgtcag cgcgggattc acaagtaatc
      541 gacgcatagc gagtcctata cttttgtcac tcaactttct ctggcacggg tcctatgtta
      601 gaagttttgt tcgaggaaat tcttactgtg aggcagcgat gactttaatt ctcaataatc
      661 gcgacgtggg ggaggcgctc aatgtaggag actgcctgaa ggtcatggag gaatcttacc
      721 gcgagcaggc cggaagccgc gcggtcaacc ggccgacttg ccattcctat ctgccgcact
      781 cactgcccaa ttcgacttac agttttaagt ctgtcgatgg tggggtcggg aaatacgggg
      841 tcctggcatt gcgcatcacg tcggacgttg ttcaggaaca agaggtccat ggcacggtgc
      901 gcttggaaaa gatcccgctg gcgggcacag gactcttcgt cggcctcgtg caactttaca
      961 gctcggagac tggcgagctg ttagccatca tgcccgacgg tcttatccaa cagactcgcg
     1021 tcggcgtcac cagcgcgctg gggatgaaag tcatggcgcg cgagaattcg cagattctcg
     1081 gcctcatcgg ttccggtggc caggccaaag cgcactaccg ttacatgacc gcggtgctgc
     1141 cgattaaaaa agtaaaagta ttcagcccca atgccgaaca ccgcaacgct ttcgtcgccg
     1201 agatggaaaa ggagaccggc gtcgccggag aatcggtggc tagcccggaa gaagccgtgc
     1261 gcggctgcga tgtcatttgc agcgcgacca attcatcgcg cccggtgatt aaaatcgatt
     1321 ggctcgagtc cggcatgcac tacaactcga tccgcgagtt tgaaactgat ctggaggttc
     1381 taaaccgctg cgatcccatc gccatccaca cccagttcgg cggcatccag cattatcagc
     1441 cgccgggaat agtcgacgac atgcccgggg tgcgccggga aaagccgcgc gactggacta
     1501 aatatcccga gatctgcgat ctcatcgccg gcaaattgcc gagccgaacg tcggacaaac
     1561 agatctcctt tttcttgaac aacgtcggca ccggagtaca gttcgctgcc atgggctatt
     1621 gcgcctacaa agccgctaaa gagaacggta tgggacgtga aattcccagc gaatggttca
     1681 tgcaggatat caagccgtga gataagtttt ttatctgccc atcatattct tgagtttatc
     1741 gaccacatcc ttcggctgcg tcataacgtc ggcggctaag gcttgcactt cctcccccgt
     1801 ggtcgggtcc atgtctaagc gctttctctt agtctcggca agaaagtcgg gatccttcat
     1861 agttttggtg aaggcgtcgc gcagcgcttt gactatttcc gcacgcattt ttggcggcgc
     1921 catatacggg cggtgaaact ctccggaggc caataacgcg gtggctaggc ggcggccggt
     1981 atccggcgtt ttgtatttgt ccatcaactc gtaaaccgtc ggcacgttgg cgagtttctc
     2041 aagcctcact tttccgcctt gggtcagttc acgtgaaaac ccattcttgc gccaagtgag
     2101 aaatggttcg cgcgaaaaat aagcctcggt gcttagcgac cggcaaacga tctcgccacg
     2161 ctccactcca agatcgatct cgttgccgcc ttgatagcca gcgatcacgt tgaatttcgc
     2221 gccgactgtt tcttcgagca gcttgggcac gaagtaaccc atattactca tacccgtagt
     2281 gccgcatttc ggcgcttctt tagcattgcg cacgtcttcg atggacttga acggcgcgtc
     2341 ggcgcgcatg tacagcacct gcggcgactt ggtagcattg ccgatccagt tgaatttggt
     2401 ccaatcgaat ttgacttcag cacggccaat caactgctcg aagtagagcc cggcgttgat
     2461 cgccccaaga gtcaagccat caggcttagc gacgttgtaa acataattag cggcaatcat
     2521 gtggcctgca cccggcatat tttggactat gatattgggg ttgccgggca agtactttcc
     2581 catgaacagg gcgatggcgc gggcataaag atcgtagaga tcgccggcaa cggtgccgac
     2641 cacgatcgtc atcgtcttgc cttgaaagtt tggagtttgg ccatgagacg gaaggacacc
     2701 catcaagtta aatgctaagc agatcacaac gagttttaat cgcatcgcat cctcccatgg
     2761 gccaaaccga tcaaactttt gaagcttgaa ccgttgaaca atttttgagc tactccgaga
     2821 gtatcttctt cacccgctcg atgacatcgg gtggctgagt catgatctcg tttacgagtg
     2881 tttgcaattc ttcgccgctc gtcgggtcca tgtccatccg gccctttttg gcttcggcga
     2941 tgagttccgg gtctttcatt gatcggccat aggcatctcg cagcgttttg acacgatcgg
     3001 gtggcgttcc aggtgccacc agcatgaaac ggccaaattc agctccaccg agtaaaacat
     3061 tggcaatgcg ccggttggtc tcggtggttt tgaattggtc catgagctc
//
LOCUS       DATLCH010000184         3170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9941242, whole genome shotgun sequence.
ACCESSION   DATLCH010000184 DATLCH010000000
VERSION     DATLCH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9941242"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..631
                     /locus_tag="VMZ02_05415"
     CDS             128..631
                     /locus_tag="VMZ02_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR71403.1"
                     /translation="MTTTGIVNFTIKPATKKDVPVILNFIKKLADYERLSHEVVATEE
                     LLQRTLFGQRRTAEVALGYVNSDPVGFVLFFHNFSTFVGRPGIYIEDLFIDEPFRRRG
                     FGGALLRHVAKLAAARECGRLEWSVLDWNEPAINFYRKLGAGSMNEWTVFRMTGENLR
                     RLADRKS"
     gene            complement(711..1382)
                     /locus_tag="VMZ02_05420"
     CDS             complement(711..1382)
                     /locus_tag="VMZ02_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HUR71404.1"
                     /translation="MKPVKADNKDAIAAAVVEERLRLSRELHDRALQLLTSARMRLDA
                     CHHYLKSDVSAVENELLTIEASLDHAITEIRNLLAENQAGDQMQAGSLERRLKEELNI
                     FSARSGFHVELQCTIDADDLPIEVEKELYFTLREGVLNAVRHARASEICLSLARIQSG
                     YRASLSDNGIGFIVAAAEGSNHYGLKGMRERAEKLGGTLDIASSPGKGTRIEIKIPFP
                     NKQAN"
     gene            complement(1443..2156)
                     /locus_tag="VMZ02_05425"
     CDS             complement(1443..2156)
                     /locus_tag="VMZ02_05425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR71405.1"
                     /translation="MATSSWQQTNSDNNAMPLYVSVPSGTGPFPAVVVIQHQTGVDEF
                     IQGIAQRLADAGYVAAAPNLYHRDGPDSQDDPRTRSQRLGDRRVIADVDATVKFLQNH
                     SAVDSKRIGIIGFCMGGRVVYLAAATNPEFKAAVSFYPGNTGRSWGRDIPSPFERTVD
                     IHCPIQGHFGDDDKNPSPEDREKLAAELTKYDKAHEFYAYAGAGHAFMDNTKDSFRPS
                     AEAQAWPRTLAFLGRYLET"
     gene            complement(2230..2952)
                     /locus_tag="VMZ02_05430"
     CDS             complement(2230..2952)
                     /locus_tag="VMZ02_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUR71406.1"
                     /translation="MAHANLSGNRTKAVFFDAAGTLIKPARRVGESYAAIAAQYGKSV
                     TPAELLERFRTCFDCAPPLAFPGVVPAEIVARERQWWKQLVAQVFEPWGEFVGFNEYF
                     DELFAYFAQPDAWLLYPEAVETLDALKQRGLVLAVISNFDSRLLRIIDGLNVGARFAQ
                     IFVSSAVGYAKPQPEIFNIALARFGLTPSQALHIGDSEINDLQGARSAGIRALLVDRD
                     LTGNIALDGRIHSLRQILDHLD"
BASE COUNT          658 a          817 c          926 g          769 t
ORIGIN      
        1 ctcaaagacg ccggcaagta gtcgagttag attttcatca caaagacaat aatcaaaatg
       61 gcgaaccagc tttccgaagc tttcgcctgc gcggtgatca cgaggaattt actttgagga
      121 tcaactcatg acgacgacag gcatagtgaa tttcactatt aagccggcca ctaagaagga
      181 tgtgccggta attctgaatt tcattaaaaa gcttgcggac tacgaacgct tatcacatga
      241 agtagtagcg actgaggagc ttttgcagcg gaccctattc ggccagcgac gcactgcgga
      301 agttgcgctc ggctatgtga acagtgaccc ggtcggattt gttttgttct ttcacaactt
      361 ctcgactttt gtcggccggc cagggatata catagaggat ctgttcatcg atgaaccgtt
      421 ccgccgacgc ggtttcggcg gcgcgctgtt gcgccacgtc gccaagctgg cagcggcgcg
      481 cgaatgcggc cggctagaat ggtcggtgtt ggattggaac gagccggcga ttaactttta
      541 tagaaagctc ggcgccggat cgatgaatga gtggacggta tttcgtatga ccggagaaaa
      601 cttgcggcgt cttgccgacc gtaagagtta aaccacgaag tttgtgtctt ctcgacgtca
      661 acttcgcgcg ctttcgattt acattaaact gtcggattga aaacgcccgt tcagtttgcc
      721 tgtttgttgg gaaatgggat cttaatctct atgcgggttc ctttgccggg actcgatgca
      781 atgtccaaag ttccgcccag tttttccgcg cgttctctca tgcctttcaa tccgtagtgg
      841 ttactgcctt cggcggcggc gacgatgaat ccgataccgt tgtcgctaag actggctcga
      901 tagccagatt ggatgcgtgc cagcgacaag catatttccg acgcacgggc gtggcgcacc
      961 gcgttgagta ccccttcgcg caaggtgaag tagagttctt tttcgacctc gattggcagg
     1021 tcgtcggcgt caatggtgca ttggagttca acgtggaatc cgctgcgtgc gctaaatatg
     1081 tttaactcct ctttaagccg acgctccaag gaaccggctt gcatctgatc cccggcttga
     1141 ttttcggcga gcaggttgcg gatttcggtg attgcgtgat cgagactggc ttcgatggtc
     1201 agaagctcgt tttctaccgc cgacacgtcg cttttcaggt agtgatggca agcgtcgagg
     1261 cgcatccgcg cgctggtgag cagctgtaaa gcgcggtcgt gaagctctcg tgacagccgt
     1321 aagcgctcct ctacgacagc cgctgcgatg gcatccttgt tatcggcctt aaccggtttc
     1381 atgtagactc tgctttgcgc tgggctcagc ggtgttccga tccgcaagcg atttcagact
     1441 gttcacgtct cgagatatcg gcccaaaaat gccagggttc gcggccatgc ctgggcttcg
     1501 gcgctcgggc gaaacgagtc tttggtattg tccatgaatg cgtgcccggc ccccgcatag
     1561 gcatagaact catgggcttt atcgtactta gtcaactccg cggcgagttt ctcgcggtct
     1621 tcgggcgatg gatttttgtc gtcgtcgcca aagtgtcctt gaattggaca atgaatatcg
     1681 acggtgcgtt cgaacggcga cggaatatcg cggccccagg agcgtcctgt gttgccggga
     1741 taaaaactta ccgcggcttt gaattccggg ttggttgcgg ctgccaaata gaccacccgg
     1801 ccgcccatgc aaaagccgat aatgccgatg cgtttgctat ctacggcgga atggttttgc
     1861 agaaacttta ccgttgcgtc gacatccgcg atgacgcgcc ggtcgccgag gcgttggctg
     1921 cgggtgcgcg gatcgtcttg agagtcggga ccgtcgcggt ggtacaaatt gggggcagcg
     1981 gcgacgtagc cggcgtcggc gaggcgttgg gcgattccct gaatgaactc atctacaccg
     2041 gtctgatgtt gaatcacgac cactgccgga aatggccccg tgccgctggg gacgcttacg
     2101 tacagcggca tcgcgttgtt atcagaattg gtttgttgcc aactggaagt tgccatcttt
     2161 cgtgcctcga attattgccg tctagcttag gtatcacgcg gagtcaaatc aacattgggc
     2221 ggtgttgtgc taatcgagat ggtcgagaat ctgccgcaag ctgtggatgc gaccgtccaa
     2281 cgcgatatta ccggtgaggt cgcggtcgac caagagcgcg cgtatgccgg cgcttctcgc
     2341 cccttgtaag tcgttgatct cgctatcgcc gatatgcagc gcttgactcg gggtcaggcc
     2401 aaagcgcgcg agcgcgatgt tgaaaatttc cggctgcggt ttggcataac ccaccgcgct
     2461 cgagacaaag atctgcgcga acctggcgcc gacgtttagc ccgtcgatga ttcgcagcag
     2521 tctggaatcg aagttggaga tcacggcgag cacgagaccg cgttgtttaa gcgcgtcgag
     2581 ggtttcaacc gcttcgggat aaagcagcca ggcatcgggt tgagcgaagt aggcaaacag
     2641 ttcatcgaaa tattcgttaa aaccgacaaa ctcaccccaa ggttcaaaaa cttgcgccac
     2701 cagctgtttc caccactggc gctcacgcgc tacgatttcc gcaggaacta cgccggggaa
     2761 ggcgagcggc ggcgcgcagt caaagcaagt acggaagcgt tctagtagct ctgccggcgt
     2821 caccgacttg ccatactggg cggcaattgc ggcatagctt tcgccgactc tgcgcgcggg
     2881 tttgatcaat gtgccggcag cgtcgaagaa aactgctttg gttctgttac cggacaaatt
     2941 cgcgtgagcc attatttcgt tttgtaacag aatgctcgcg ctgctgtgag ccagcgctcg
     3001 agttggtttg gaccgtacca tcctttataa ttgaaatcga tgagcaaaaa agccgccttg
     3061 atagtagtcg atgtgcaaaa cgatttttgt ccgggcggtt cgttggggat cgccgacggc
     3121 gatcgggtcg tgatagcgat taatcgatat gtcgagcagt ttgccgctgt
//
LOCUS       DATLCH010000185         7871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1252674, whole genome shotgun sequence.
ACCESSION   DATLCH010000185 DATLCH010000000
VERSION     DATLCH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252674"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VMZ02_05435"
     CDS             complement(<1..526)
                     /locus_tag="VMZ02_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUR71407.1"
                     /translation="MKKSLQLVMFDLDGTLADTGHDLADSVNFTRREFELAPLPDHAV
                     YANVGRGIEHLLKRSLPEEASDKFPQVLRIFLTYYEAHLLDRTVLYQGAHDVLRYFAS
                     KRRAVVSNKLHRLTLAVVQGLGVAEQFDAILGGDSVAEKKPHPAMLNAVLQQFQISPS
                     DALIVGDGDTDIEAG"
     gene            646..1641
                     /locus_tag="VMZ02_05440"
     CDS             646..1641
                     /locus_tag="VMZ02_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71408.1"
                     /translation="MALNRAAGTLTLVVIAVLALANIAAAQKLNTAYISTTPGTSTVI
                     QVAKEARLFDKHGIDATVIFISGSVRGIQAILAGEIPIGEGGGPGLASARIAGGDVVA
                     IAGNVNVLPYYLVSHPSIKRHEDLKGKIGGTHIAGTTAEFALKVGLKKIGLDPLKDTS
                     LRVIGGAIERMVALQKGIVQFTVVTEAGKAQAEKLGYPTVVDMVALQIPFPQNGIYTS
                     TKLIRENPDLVRRYMRAYVEALHFYKNQKEDTFKIMRKYSRVEDRNVLEVAWDWHARF
                     MPEAPYPPVEGYQLVLNDIAEKNPKAAQANVKEYIDARFVKELEDSGFIKGLYRK"
     gene            complement(1732..2187)
                     /gene="grdA"
                     /locus_tag="VMZ02_05445"
     CDS             complement(1732..2187)
                     /gene="grdA"
                     /locus_tag="VMZ02_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_081459675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2062..2064),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HUR71409.1"
                     /translation="MQKDRKIIAIGERDGVPGPIIAECLEAAGYEVVFQATECFVUTA
                     AGAMNFENQGVIKRLADQYGPENLTVVLGNGEANGVEVFAETVTEGDPSFAGPLAGVA
                     LKIPVYHMLEADVMGQVPESLRDEKLQLSSLVIDIEPMHQVLEASRAKL"
     gene            complement(2198..3628)
                     /gene="grdC"
                     /locus_tag="VMZ02_05450"
     CDS             complement(2198..3628)
                     /gene="grdC"
                     /locus_tag="VMZ02_05450"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF040746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HUR71410.1"
                     /translation="MSVSEKNLKPLRVGIEGVSYSLAHVPDLVRFGSKPLRELRGDPA
                     LAANFAKLLRDFPAAIGYAPHQVYIGNIDPVTLRNLPQPWYEKLIEGAMARGRFGDLV
                     EQAKFYALLAGADGFDLVCFEEKWFQAHAAKYSIGRPPRLKTLADIQNLCDKGALSLY
                     SGPQLVGAFESAHEEDASLAAEVLLENLSGKVSAAHALRLVLAQLELKADDVDFVISC
                     SEEAVGDRYNRGGGNLAKAIAEDVGCGNASGCDVKAFCAGPMYAMVQAASMIQAGVAK
                     KVAVVAGGSLAKLGMKFESHVKKNMPILEDMIGSFAAILSLPKAGQVCMRLDSAAFHP
                     VRAGGSPQAMAEELAEKPLAKLGMRLTDVDRYGVELHNPEITVPSGSGDVPRTNYLTL
                     AALAVMRGQIPKNQMDDFVRSRGMLGYAPTQGHIPSAIVYLGHAKAALEKGEIRNALL
                     MAKGSLFLGRMTQLSDGCSFLLDRNE"
     gene            complement(3651..4205)
                     /locus_tag="VMZ02_05455"
     CDS             complement(3651..4205)
                     /locus_tag="VMZ02_05455"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HUR71411.1"
                     /translation="MAIVQTFLAGRVAVKVGDITEEAVDAIVNAANGSLMGGGGVDGA
                     IHRAGGPAIKAACAELRRTQYPRGLPTGEAVITTAGKLPARYVIHTVGPVYGSGGADK
                     ADLLAACYFNSMSLATLHKLNTIAFPAISTGIYGYPQAEAAVVSSRTIEQFLAHNNSI
                     AALNLLFFSAEDAAVFLQHQVFSG"
     gene            4455..5129
                     /locus_tag="VMZ02_05460"
     CDS             4455..5129
                     /locus_tag="VMZ02_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71412.1"
                     /translation="MAGLNRSDEAIGTRDAEPRWPAFLAILAVGGLYAALPDVLTMGP
                     NWFFPSIVFVLLIPNIVSHHTGRRQLNNVLGFAVDGVLTLGLIFSLGLLIAALPTHRQ
                     SPMALLLSAASLWVTNIIVFALWYWRLDAGGPNQRDKRTEHFAGAFLFPQMTMNNQSL
                     RQIGQVGWSPNFVDYLFLSFNTSTAFSPTDVPVLARWAKVLMMIQSLLSLLIIALLAA
                     RAINIL"
     gene            complement(5145..5876)
                     /locus_tag="VMZ02_05465"
     CDS             complement(5145..5876)
                     /locus_tag="VMZ02_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71413.1"
                     /translation="MAVETFLVRTMSDTVETDIERVHVIANEILPRYNLPPLTFEQLR
                     KVADGPFDLFLIPHVFAAEYAKDRNLPFNEAKRVEIRRIALQIARQHGYDVDPPPFQT
                     GIERSLKETKEAGLRNILMTTGGRRYKHQAMERFGIGGYFEEIVDREQTYYAKEQGFY
                     YLYRKQKMPHIRIVVLSGTANYIKAANNVNGCQVGDTFLEVVPIALATPHSYSDEETL
                     RAAAPRAVIHGFAELLPKLNELAPG"
     gene            complement(6024..6296)
                     /locus_tag="VMZ02_05470"
     CDS             complement(6024..6296)
                     /locus_tag="VMZ02_05470"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUR71414.1"
                     /translation="MEPSPFLVEVSEAEIRSERQKARELRQSQWWKRKRSSGICHHCA
                     GKFAPAELTMDHLIPVVRGGKSTKGNVVPSCKACNNARKHNLPFEK"
     gene            complement(6309..6497)
                     /locus_tag="VMZ02_05475"
     CDS             complement(6309..6497)
                     /locus_tag="VMZ02_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71415.1"
                     /translation="MKLLTAHKILITSAVIFFVFFSGWEYRNFAAGDAWAAWRSGLYL
                     IVAGGFVVYLKNLKRWYR"
     gene            6616..6996
                     /locus_tag="VMZ02_05480"
     CDS             6616..6996
                     /locus_tag="VMZ02_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUR71416.1"
                     /translation="MIRETISTDKAPKAIGPYEQAVRVGDIIYTAGQIPIDPTTGNLI
                     DGGIVEQTRQVLENLKAVLEAGGASLDTVIKATVFLKNMADFAALNEVYGLYLGKAKP
                     ARSTVAVADLPRGALVEIDMVATI"
     gene            complement(7096..>7871)
                     /locus_tag="VMZ02_05485"
     CDS             complement(7096..>7871)
                     /locus_tag="VMZ02_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUR71417.1"
                     /translation="FATFTTLTAYYAGSGLILGSLGAKKLDTQIAEQRELRNIVSEMA
                     LASGARMPQVYVIDDAAANALATGTYEKNAAICVTTGLLSLLDREETQGVVAHEMSHI
                     RHQDTLLMTLVSVLVAGIAILADWGQRGAYHARRSRGDGRKNPALFIPLLLLVAVSPI
                     IAQLLAMAVSRQREYLADATAAQYTRNPLGLARALEKIRDAAVPVNKATRGTAPLFFS
                     DPLRRRLGDSESGLANLLTTHPPIGKRIRLLYQMAGLAR"
BASE COUNT         1814 a         2246 c         2060 g         1751 t
ORIGIN      
        1 tgccggcttc gatgtcagtg tcgccatcgc cgacgatcag cgcatctgac ggggaaatct
       61 ggaattgttg caacactgca ttcaacatgg ccgggtgagg tttcttctcg gcgacactgt
      121 cgccgcccaa aatcgcgtcg aactgctctg ccacgcccaa cccttgcacg actgcgagcg
      181 tcaaacggtg caatttgttg ctcaccaccg cgcgccgctt cgaggcaaaa taacgcagaa
      241 cgtcgtgcgc gccctgataa agcaccgtcc gatcgagcaa gtgcgcttcg taataagtca
      301 gaaaaatccg cagaacttgg ggaaacttat ccgaagcttc ttcgggcaac gatcgtttga
      361 gtagatgctc gattccacgc cccacattcg cgtagacggc atggtcgggc agcggagcaa
      421 gctcgaactc gcgacgcgta aaattgaccg agtcggccaa gtcgtggccg gtatccgcga
      481 gcgtgccgtc gagatcgaac ataaccaatt ggagggattt tttcatcgag agtcagcaaa
      541 atagaccgca cctttgtctc tgtcaaatca gctttgccaa cttagatgaa agtgatactc
      601 atgagtggaa ttaagttacc cagcaaaacc tttggaggtc gttgtatggc tctgaatcgc
      661 gccgccggta cattgacttt ggtcgtcatc gcagtgctcg cgctggcgaa tatcgccgcc
      721 gcccagaagc tcaacaccgc ctatatctcc accacgccgg gaacctcgac cgtgatccag
      781 gtcgccaaag aagcgcgtct gttcgacaaa cacggcatcg acgccaccgt aattttcatc
      841 tccggcagcg tgcgcggcat tcaagccatt ctcgccggtg aaattcccat cggcgaaggc
      901 ggcggccccg gactcgcaag cgcgcgaatc gccggcggcg acgtcgttgc cattgccggc
      961 aacgtcaacg tcttgcccta ctatttagtt tcccatccaa gcattaaacg gcatgaggac
     1021 ttgaaaggta aaatcggcgg cactcacatc gccggtacga cggcggagtt cgccctgaaa
     1081 gtcgggctaa agaaaatcgg tctcgacccg ttgaaagata ctagtctgcg cgtcattggc
     1141 ggtgccattg agcgcatggt cgcgctgcaa aagggcatcg tccaattcac cgtggtcacc
     1201 gaagccggca aagcccaggc ggagaagctc ggctacccca ccgtcgtcga tatggtcgca
     1261 ttgcaaattc cgtttccgca aaacggcatt tacacgtcga ccaagttgat tcgtgagaat
     1321 ccggatctag tgcgccgcta catgcgggcc tatgtcgagg cgctgcactt ttacaaaaat
     1381 caaaaggaag acacgttcaa aataatgcgc aaatactcgc gcgtggagga ccgcaacgta
     1441 ttggaagtcg cttgggactg gcacgccagg ttcatgcccg aggcgcccta cccacccgtc
     1501 gaaggctacc aattggtgct gaatgatata gcggaaaaga atcccaaagc tgctcaggcc
     1561 aacgtcaaag aatatatcga cgcgcgtttt gtgaaagagt tggaagattc gggatttatt
     1621 aaggggctgt atcggaaatg accgttccaa aggttccaat cgttgcacgg gttccaatcc
     1681 ggaccgaaaa ccgttgaaac atttggaacg actgaaacga ttggaaccat cttataattt
     1741 cgcccgcgaa gcttctaaaa cttgatgcat tggttcgatg tcgatgacga gcgacgacag
     1801 ctgcaatttt tcgtcacgta acgattccgg cacctggccc atgacgtctg cttcgagcat
     1861 atggtaaacc ggaatcttca gcgcgactcc agccagtggc ccggcgaaac tcggatcgcc
     1921 ttccgtcact gtctcggcaa acacttcgac tccgttagcc tcgccgttgc ctaataccac
     1981 ggtcaagttt tccggaccat actggtccgc cagacgcttg atgactcctt gattctcgaa
     2041 gttcatcgcg cctgcggcgg ttcaaacaaa gcactcagtg gcctgaaata cgacttcata
     2101 tcccgcagct tcgagacact cggcgataat cggacccggc acaccatccc gttcgccgat
     2161 ggcaataatc ttcctatcct tttgcatttt tcctccacta ttcgttgcgg tcgagcaaaa
     2221 acgaacaacc gtccgacagc tgggtcatcc ggccgagaaa caaactccct ttggccatga
     2281 gtagcgcatt gcgaatctcg cccttctcga gcgccgcctt agcatggcct aaatacacta
     2341 tcgccgaagg aatatgtccc tgcgtcggcg catagcctaa catgccgcgg ctgcggacga
     2401 aatcatccat ctgattcttg ggtatttgcc cgcgcatcac cgccagggca gcaagagtga
     2461 gataattcgt ccgcggcaca tcgccgctgc cggagggcac ggtgatctcc gggttatgca
     2521 gctcgacacc gtaacggtcg acgtcagtca atcgcatgcc gagcttggcc agcggcttct
     2581 cggcaagttc ctcagccatt gcttggggcg agccgccggc acgcactgga tgaaacgccg
     2641 cagagtcgag tcgcatgcaa acctgtccag ccttgggcag gctgaggatc gcagcgaaac
     2701 tgccgatcat gtcctctaag atcggcatat tctttttcac atgggactca aacttcattc
     2761 cgagtttagc cagcgatccg ccggcaacca ccgcgacttt tttcgccacg ccggcttgga
     2821 tcatgcttgc cgcctgtacc atggcgtaca tcgggccggc gcaaaatgct ttgacatcgc
     2881 atccggaagc attgccgcaa cccacgtcct cggcgatcgc ctttgcaagg ttgccgccac
     2941 cgcggttata gcgatccccc accgcttctt ccgagcaact gatgacaaaa tcgacgtcgt
     3001 cggctttgag ttcgagctgg gccagtacga ggcgtagggc gtgagcagcg gaaactttgc
     3061 cggataaatt ttccagcaac acctcggctg cgaggcttgc gtcctcttcg tgcgccgact
     3121 cgaaggcgcc gacaagctgc ggtccagaat aaagcgacag ggcacccttg tcgcagagat
     3181 tttgaatatc ggccagagtt tttaagcgcg gcggcctgcc gatggaatac ttggcggcgt
     3241 gagcttgaaa ccacttctcc tcgaagcaca ccaaatcgaa gccgtcggcg ccggcgagta
     3301 aagcataaaa cttcgcctgc tccaccagat cgccgaacct gccccgcgcc atcgcgcctt
     3361 cgatcaactt ctcgtaccag ggctgcggca aattgcgcag cgtaaccggg tcgatattgc
     3421 cgatatagac ctgatgcggc gcatagccga tggccgccgg aaaatcgcgc aagagcttgg
     3481 caaaattggc tgcgagcgca ggatcaccgc gcaactcacg caacggtttc gaaccgaagc
     3541 gcaccagatc cgggacgtga gccagtgaat aggaaacacc ctcgataccg actcgtagtg
     3601 gttttaaatt tttctcactc acgctcatcg cctctaatct tgatttacat ctatccgcta
     3661 aagacttggt gttgcaagaa caccgcggca tcttcggcac tgaaaaaaag caagtttaag
     3721 gcggcgatgg aattgttatg cgccaaaaac tgctcgatcg tacgcgacga caccactgcg
     3781 gcctcggcct gaggatagcc gtagattccg gtggagatcg ccgggaaggc gatcgtatta
     3841 agcttgtgta aagtcgccaa actcattgaa ttaaaatagc aggccgccag cagatccgcc
     3901 ttatccgcgc cgccactgcc gtagaccggc ccgacggtgt gaatcacata acgcgccggc
     3961 aatttgcccg cagtggtaat cacggcttct ccggtcggca agccgcgcgg atattgtgtg
     4021 cggcgaagtt ctgcacaagc ggcttttatc gccggcccgc cggcacgatg aatcgcaccg
     4081 tccacaccgc cgccacccat tagcgagcca tttgccgcgt tgacgatggc atcaactgct
     4141 tcctcggtga tgtcaccgac tttcaccgcc acccggccgg cgagaaatgt ttgcacgatc
     4201 gccactcttc ctccgtctgt ttcatcagcg ccaccgacga cttaagttag tgcaggaagt
     4261 tgcaagtctc aagcgattgc cttgactcgc tcaagccaac cgcgcaaaag taccaacgaa
     4321 cccggttttt ccgaaagggg actaacgatg catgtttact gtgttgccta acaactaacc
     4381 ggcgattcgg tgaactcggt cggctaactg ggcatcaata accagcgtag ctggagaaat
     4441 tgtcgaggta ctttttggcc gggttaaatc gcagcgacga agcgatcgga actcgagatg
     4501 ccgagccacg ctggccagct tttttagcca ttctggcggt cggtggcctg tatgcggcgc
     4561 tcccggatgt cctcaccatg ggacccaatt ggttttttcc gtcgatcgtt ttcgtgctgc
     4621 tgattcctaa tatcgtttct caccacaccg gccggcgtca acttaacaat gtgctcggct
     4681 tcgcagtcga tggcgtgctc accctgggtc taattttctc tctcggtctt ctgatcgcag
     4741 cgctgccgac acaccgacaa tcacccatgg cgctgctgct gtcggcggca tctctctggg
     4801 tgaccaatat tatagtattc gcgttatggt attggcgatt ggacgctggt ggtccgaatc
     4861 agcgcgataa acgaacggag catttcgcgg gggcattcct gtttccgcag atgaccatga
     4921 acaatcaatc cttgagacag atcggccaag tcggctggtc gccgaatttt gtcgattacc
     4981 tgtttttatc cttcaacact agcacggcat tttcgcccac cgatgtacct gtgctggcgc
     5041 gctgggcaaa agttttaatg atgatccaat cgcttctttc tcttcttatc attgcgctgt
     5101 tggcagcacg cgccattaac atcttataat cgcgcagagt ccatttagcc cggcgcgagt
     5161 tcattaagtt ttggcagcag ttcggcgaat ccatgaatca ccgccctcgg agctgcggcg
     5221 cgcagagtct cctcatcgct ataagaatgg ggtgtcgcta aggcgatcgg aacgacctcg
     5281 agaaaggtat cgcctacctg gcagccattc acgttattag cggctttgat gtaattcgcc
     5341 gtgccgctca gtacaacgat acgaatatgc ggcatttttt gcttgcgata gaggtagtag
     5401 aaaccctgct ctttggcata ataggtttgc tcgcgatcga cgatttcctc gaaataaccg
     5461 ccaatgccga acctctccat tgcctgatgc ttgtaacgcc ggccaccggt ggtcatcaga
     5521 atattgcgca gaccggcttc cttagtttct tttaacgatc gctcgatccc cgtctgaaac
     5581 ggcggcggat cgacgtcata gccatgctgg cgcgcgatct gcaacgcaat tcgacgaatc
     5641 tccactcgtt tggcttcatt aaatggcagg ttgcgatcct ttgcgtactc cgcggcaaaa
     5701 acatgcggta tcaaaaacag atcgaacggc ccatcagcga ctttgcgcaa ttgctcgaaa
     5761 gtcagcggcg gcaggttgta gcgaggcaaa atttcattgg cgataacatg aacccgctcg
     5821 atatcggttt cgactgtatc ggacattgtg cgcacgagaa atgtttcgac cgccatgact
     5881 ttgccctccg tcgaacgata ctagtgaagc cggcttctag tggcaagggt tggatgtttc
     5941 gagatcagag attccacata gcaatttaca gcattccagc aaataggtga tctgaactct
     6001 atgatttctg gaatgcgaaa tagctatttt tcgaaaggta aattgtgctt acgagcgttg
     6061 ttgcacgcct tacaactcgg aaccacattg cccttagtcg acttgccgcc acggaccacc
     6121 ggaataagat ggtccatggt caactccgca ggcgcaaact tgccagcgca atgatggcaa
     6181 ataccgctcg atcgtttacg cttccaccat tgactttggc gcagctcgcg cgccttttgc
     6241 cgctcgctgc gtatctctgc ttctgacact tcgacgagga aaggagaggg ttccatcaaa
     6301 attgtttctt agcggtacca gcgtttgaga ttcttcagat agacgacaaa tccgccggca
     6361 acgatcagat ataaaccgct gcgccaggca gcccaagcat cgccggcggc gaagttgcga
     6421 tattcccagc cactgaagaa aacaaagaag attaccgcac tggtaattaa tattttgtgc
     6481 gcagtgagca gtttcatgac tgccgctaat caatcatagc gagaccgttc agttcaagtg
     6541 cgcgagccgc caatcgttgc gccagattca gttaatgtta tagctcgtaa gtgtcagcta
     6601 acagggagca tacgaatgat tcgagaaacc attagcaccg ataaagcgcc caaggccatc
     6661 ggtccctacg aacaagccgt tagagtcggc gacatcatct acactgctgg gcaaataccc
     6721 atcgatccca cgaccgggaa tttgattgac ggcgggatag tcgaacaaac gcgccaggtt
     6781 ttggaaaatc tcaaagcagt gttggaagcc ggcggcgcat ccctcgacac ggtgatcaaa
     6841 gccacggtgt tcttaaaaaa tatggctgac ttcgcggcct tgaacgaagt ttacggtttg
     6901 tatttaggaa aggcgaaacc agcccgttca acggttgcgg tcgccgattt gccgcgcggc
     6961 gctctggttg aaatcgacat ggtcgcaacg atctgaaggc agtcgaaggg aacggtgagt
     7021 ttttaaacac acatcgctcc gacacctacc cacgacggcg cgagctgctg tccgtaaata
     7081 ctcggtattt tgccgtcagc gcgccaatcc cgccatttgg taaagcagcc gaattctttt
     7141 tccaatcggt ggatgggttg ttagcaagtt cgccaagccg ctctcactgt cgcccaatcg
     7201 tcgtcgcagc ggatcactaa agaagagcgg cgctgtgccg cgcgtcgctt tgttgaccgg
     7261 cacagcggca tcgcgaatct tttcgagcgc ccgcgccaag ccaagcggat tacgcgtgta
     7321 ttgggccgcg gtggcatcgg ctagatattc cctttgccgt gatactgcca tggccaacag
     7381 ctgagcaata atcggcgaca ccgcgacaag cagcaaaagc ggaataaaca gcgcgggatt
     7441 cttacgacca tcgccgcgcg atctccgcgc atgataggcg ccgcgctgcc cccaatccgc
     7501 taggattgcg atacccgcaa cgagaacgct caccagcgtc atgaggagag tgtcttggtg
     7561 acggatatgc gacatctcgt gtgccaccac gccctgagtt tcctcgcgat cgagcaagct
     7621 aagcagaccg gttgtgacgc agatggcggc atttttctca taggtaccgg tggcgagcgc
     7681 gttggccgcg gcgtcatcga tgacgtagac ctgcggcatc ctggctccgg atgccaatgc
     7741 catttcgctg acgatgttgc gcaattcgcg ctgctcggcg atctgggtat ctaatttttt
     7801 cgcgcccagg gagccaagga taaggccgct gccagcatag taagccgtaa gcgttgtaaa
     7861 ggtcgcgaaa c
//
LOCUS       DATLCH010000186         7600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2744282, whole genome shotgun sequence.
ACCESSION   DATLCH010000186 DATLCH010000000
VERSION     DATLCH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744282"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..459)
                     /locus_tag="VMZ02_05490"
     CDS             complement(61..459)
                     /locus_tag="VMZ02_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71418.1"
                     /translation="MRIFCVLLLFLGMAGGCADKIDHDTALATKRALEFAEATFVRGN
                     LDQGYGMLADKARNYIPMNIFADKVKQMHPSGRPNKIAGLSAAPVKGEKLVTVTVRGE
                     GDGQFLYTIAVLGTATDDYRVTTFSGGRSS"
     gene            complement(540..1907)
                     /locus_tag="VMZ02_05495"
     CDS             complement(540..1907)
                     /locus_tag="VMZ02_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUR71419.1"
                     /translation="MPKYKEPALCGKHQVYENRRAKSGRVIALNIVVLPARSSEPKAE
                     PVFYLAGGPGQGAARIIGAGEDAIMRELRRDRDLVFVDMRGTGDSNGQQCDFPSDRGN
                     FQNFFSELFDPAIIQRCREKLEQFADLSFYNTALGVEDLAEVRLALGYDKINLYGISY
                     GSQAALEYLRRYPEHVRSAILAGVTTPAAKIPLHFAQGAEQAISRLLQDCAADDVCNS
                     AFSNLEAKFTRLLRSFDSGAVTFQVFHPTSKAMQAVTLSRGVFAVRLLSLLYSHRTLS
                     LLPLILDSAAQGDWLPYVQVVSRTAAPAEFRVYLGAYLSVTCSESLPFIDEAELVRAT
                     GGTFMGDYRTRRHQQACAHWPRGEIDAQYFEPVQASTPILMLSGDIDPVTPAEFGARA
                     LKTLPNGRRVILRNTPHSYTSPCARSLIVQFTASASAKELDASCASRLRRPPFATELP
                     PSYNR"
     gene            complement(2065..2898)
                     /locus_tag="VMZ02_05500"
     CDS             complement(2065..2898)
                     /locus_tag="VMZ02_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71420.1"
                     /translation="MNITDAQVHVWQAERPERPHIKEDASKPHQAVPLTYDRLLDEMN
                     GADVDRAILVPPSWDGYRNDYALEAAQKHPDRFAVMGKVPLNDVASRDKLPTWLEQPG
                     MKGFRISFRHSGTHSFLDDGTADWFWADCERYDIPVMIFAPFAVTAIGKIAERHPGLR
                     VIVDHMGLNVQWKGKDLAPGIDLLLTFARMSNVAVKTSCLPCYVAENYPFPTLHRHIH
                     RVFDAFSPQRIMWGTDLSQSPVTYRQNVTLFTEELKFLSESDKEWIMGKTIAHWLRWP
                     I"
     gene            complement(2962..3963)
                     /locus_tag="VMZ02_05505"
     CDS             complement(2962..3963)
                     /locus_tag="VMZ02_05505"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71421.1"
                     /translation="MFSVTAGDAIRRQLTGLIVVGCLLTALSPEKSSAQSSRQSRPLR
                     VAYLSTSSTMAPLWMAKDSGALAKENLEIEVLSMQSTSAIPALLANEIDVVEVSAAPV
                     VTAALRGIDVTFIAGLLNTMIWDFYARPEIKNAEQLKNKIIGTDRPGTPVAYGTMVAL
                     KRMGLTPKDVQLRPLGSSPQIVAAFYAGQIAGGVGSPPASFRMERDGFHSLVSLIGEP
                     YQNVGLVVRKSRIDELNAKLLPLLRAVRSGIERFYADKSFSMKVIGKYAYETDPDVLD
                     RTYDFFKKAGFRRELVTSEPGLQGVLDFLSESIPEAKSAKPTQFFDDRVLRQVNAGK"
     gene            complement(4557..5342)
                     /locus_tag="VMZ02_05510"
     CDS             complement(4557..5342)
                     /locus_tag="VMZ02_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR71422.1"
                     /translation="MSKTLAALMLLLSALNSGPAYSACRELDVARSWFKPAYAAEPDV
                     TIEFFGHNFFQITSSRGTKIITDPVTPGFYPTPELSPHAITVGREHPNHNFVEIAKNG
                     PVILRGLGNYGAEWNKVSTTVKDVLVYTVPIYQQQFGNALKGAAFVFDLGTICVAHLG
                     DLAHKLTEEQIKAFGKVDIAMIPIGGMFTMPPDTAREVLQQLKPKIAIPMHYRENTAL
                     LDMFVKGFPNKYLNSNKSTFSKSVLPPPTQVVVFTPWQMRDYR"
     gene            complement(5352..6110)
                     /locus_tag="VMZ02_05515"
     CDS             complement(5352..6110)
                     /locus_tag="VMZ02_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR71423.1"
                     /translation="MISLSLRKLTKHFGGHAAVDQANLDVNAGEFMVIVGESGCGKTT
                     TLRLVAGLESPDSGNIFIGGVSVNDVPVGKRNVQMIFQNYALWPHMKVFEESKYSNLS
                     LPLKVRQWSSEKIAEYLRPLAWKIGIDETFFKRKPSELSGGQQQRVALGRAMVTAPQI
                     MLMDEPLSNIDPPNRLKMRQEILSFHKENRLTTLYVTHNLADGVALADRIAVMHDGRF
                     EQADTPENLVRHPATEYVADFFRAEQFGPRFAAG"
     gene            6309..7061
                     /locus_tag="VMZ02_05520"
     CDS             6309..7061
                     /locus_tag="VMZ02_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR71424.1"
                     /translation="MVGLLEPEALIADLQQTLQQRHPRPHPIRQLLLSGRLTKEQLQW
                     WARNQFHEFRNIHRFFGIRYHKCPIPELRRMLLENMVEEEGEDLFGGKYPSHRELWVQ
                     FANGIGVASEDILNYEPLPGIRAALEMYVSLVQQSHWAVAIGTGLVFEGGGPKRMKEE
                     REAIEKYYSWVPVDCLDFFRAHEYHDEGHGDMVTNVIKQHCMEEKLQAEMREAVRQRG
                     DIMWLQNDCIYNAFVRPQLPGEVIREIEGRLE"
     gene            7097..>7600
                     /locus_tag="VMZ02_05525"
     CDS             7097..>7600
                     /locus_tag="VMZ02_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUR71425.1"
                     /translation="MTEKIIDFRSDTVTKPSAAMRRAMAEAEVGDDVYIEDPTVNRLQ
                     DRAAEIFDRQAGLFVPSGSMGNLTCIMAQTARGQEVVCDAAGHIYNYEMASMAALAGV
                     LPRVISTSDGILTWEDIAPAIREKTYTRPQTALIALENTHNMAGGTVYPTRLAHAICD
                     KAHESGLK"
BASE COUNT         1697 a         1987 c         2221 g         1695 t
ORIGIN      
        1 tttggcccgt cgatacgcga cgagcagcag cgccggcagg cgcaggcctt cggtcgataa
       61 ctaggaactg cgaccgccgc taaatgtggt gacgcggtaa tcgtccgtcg ccgtaccgag
      121 caccgcgatg gtgtacagga attgtccatc gccttcgcct ctgacagtga cagtgacgag
      181 tttttcgcct ttcaccggcg cagcgctgag accagcaatc ttattcgggc gtccggaagg
      241 atgcatttgt ttgaccttat cggcgaaaat attcatgggt atgtagttgc gcgccttgtc
      301 ggcaagcatg ccgtaacctt gatcgagatt gccacggaca aaagtcgcct cggcgaattc
      361 cagggcgcgt ttggtcgcca gggcggtatc gtgatcgatt ttgtcggcgc aaccgccggc
      421 cataccgaga aacagtagaa gcacgcaaaa gattctcata ataacttttc tccgtcgacg
      481 cggtgttgca tttctcgagc gcgcgaatcg ctccgagagc aatcctgaac cactcctgct
      541 tagcggttat aacttggcgg cagctcggtt gcgaacggtg gacgacgtaa gcgcgacgcg
      601 caactggcat ctaattcttt cgccgaggcg ctggcggtga attggacaat cagcgagcgg
      661 gcgcaaggcg atgtgtagct atgcggcgtg ttgcgtaata tcactcgacg gccattgggc
      721 agtgtcttga gagcccgggc gccaaactct gccggcgtca ccgggtcgat atcgccggag
      781 agcatgagta tgggtgtaga agcttgcacc ggctcaaaat attgcgcgtc gatctcgccg
      841 cgcggccaat gggcgcaggc ctgctggtgg cggcgcgtcc gatagtctcc catgaacgtg
      901 ccgccagtgg cgcggactag ttccgcttcg tcgatgaatg gcagcgactc cgagcaggtg
      961 acgctcaaat aggcgccaag atacacgcga aattccgccg gcgcagctgt ccttgagacc
     1021 acttgcacat aggggagcca atctccctgg gcggcgctgt cgagaatcaa aggtaagaga
     1081 ctgagcgtgc ggtggctata gagcaatgat aaaaggcgga cggcaaaaac gccgcgcgac
     1141 agtgtgaccg cttgcatggc tttgctggtt ggatgaaata cctgaaaagt caccgcgccg
     1201 ctgtcgaagg atcgcaagag ccgggtaaat tttgcttcca gattggaaaa agcgctattg
     1261 cagacatcgt ctgcggcgca atcctgtaac agtctggaaa tagcctgctc cgcgccttgg
     1321 gcgaaatgga gcggtatttt cgctgccgga gtagttacgc cggccagaat cgcacttcgc
     1381 acgtgttcgg gataacggcg gagatattca agggcagcct gggaaccata ggagatgccg
     1441 tataagttga tcttgtcgta gcccaaggcg agccgaacct ctgcgagatc ttcgacgccc
     1501 agcgcagtgt tataaaacga tagatcggca aactgttcta atttctctcg gcaccgttga
     1561 atgatcgcag gatcgaataa ttcactaaag aagttctgga aatttccacg atcgctaggg
     1621 aagtcgcatt gctggccgtt cgaatcgccg gtaccgcgca tgtcgacgaa gaccagatca
     1681 cgatcgcggc gcaactcgcg catgatcgca tcttcaccgg cgcctatgat acgcgccgca
     1741 ccttgtcccg gtccgccggc taaataaaaa accggctcgg ccttgggttc agaacttcgc
     1801 gccggcagca cgacaatatt tagcgcgatc acgcgaccgg atttggcccg gcgattttcg
     1861 tagacttgat gtttaccgca gagcgcgggc tctttgtatt tgggcaaatt gcatgagttt
     1921 aattctatag tggcttgtct tctggtgacg ctcggagaac ttgccacagt cgcgcaagag
     1981 gaagcaatca acagcgcgag catcactgcg cacggataat tttgcagaca ttgtttggag
     2041 agcagtacgg ccaagtctgc cttgctagat cggccagcgc agccagtgcg cgatcgtctt
     2101 gcccataatc cattctttgt cgctttccga aagaaatttg agttcttcgg tgaacagggt
     2161 gacgttttgc cggtacgtaa ccggtgattg cgacagatcc gtgccccaca tgatccgctg
     2221 cggcgagaac gcgtcaaaga ctcgatgaat gtgccggtgc aaagtgggaa atggatagtt
     2281 ctcggctaca taacagggga ggcaggaagt tttgaccgcc acgttggaca tgcgagcgaa
     2341 ggtaagtaac aggtcgatgc ccggcgctag atctttacct ttccactgga cgttcaaacc
     2401 catgtgatcg acgatcacgc gcaggcctgg atggcgttca gcgatcttac cgatggcggt
     2461 tacggcgaag ggtgcaaaga tcatcactgg aatatcgtag cgctcgcagt ctgcccaaaa
     2521 ccaatcggcg gtgccgtcgt caagaaacga atgggtgccg ctgtggcgaa agctgatgcg
     2581 aaaccctttc atgccgggtt gttcgagcca ggtcggcagt ttgtctctgc tcgctacgtc
     2641 gttgagcggg accttaccca tcaccgcgaa acggtcggga tgtttttgcg cggcttcgag
     2701 cgcgtagtca ttgcgatagc cgtcccatga tggcggcacc aagatggcgc gatcgacgtc
     2761 ggcgccgttc atttcgtcga gcaaacgatc gtaggtgagt ggcactgcct gatgcggttt
     2821 ggacgcgtcc tctttgatgt gcgggcgctc gggcctctcg gcttgccaga cgtggacttg
     2881 ggcatcggtg atgttcataa gaggccgagg tcaacagaca acaggcgatg gtaaagatca
     2941 gacgaatgca tcacgaacgg attactttcc ggcgttgacc tggcgcagga cgcgatcgtc
     3001 gaagaactgc gtcggcttcg cggacttggc ttcagggatc gattccgaga gaaaatctag
     3061 cacaccttgt aaccccggct ccgaggtcac caattcgcgg cggaaaccag cttttttgaa
     3121 aaaatcgtag gtgcggtcga gtacgtctgg atcggtttcg taggcgtatt tgccgatgac
     3181 cttcatggag aagcttttat cggcgtagaa acgctcgatc ccgctgcgca cagctctcag
     3241 caaaggtaaa agcttggcgt tcaactcgtc gatgcggctt tttcgcacca ctaagccgac
     3301 gttttgatag ggttcgccga tcaagctgac cagcgaatga aagccgtccc gctccattct
     3361 aaaacttgcc ggcggcgaac cgacaccgcc ggcgatctgc cccgcgtaaa atgccgcgac
     3421 gatctgcgga ctgctaccga gcggccgaag ctgcacgtcc ttcggcgtga gacccatcct
     3481 tttcaaagcc accatggttc cataagcaac aggagttccc ggtcgatcgg taccgataat
     3541 tttgtttttc aattgctcgg cgtttttgat ctccggacgg gcgtaaaaat cccaaatcat
     3601 ggtattcaac aagccggcga tgaacgtcac gtcgatgccg cgcagggcag cagtgactac
     3661 gggagcggca gagacttcaa caacatcgat ttcgttggcg agcagtgccg ggatagcact
     3721 ggtggattgc atggaaagca cttcgatttc caaattttcc ttggcaagcg cgccagaatc
     3781 tttggccatc cagagcggcg ccatggtcga tgaagtgctc aaataagcga cccgcagagg
     3841 ccgggattgg cgcgaagact gtgccgagct cttctcgggc gataacgcgg tgagtaagca
     3901 tccaacgacg attagccctg tcagttgtcg gcggatcgca tcacccgcgg tgacagaaaa
     3961 catccgatgc cctccaagct tggaacatat ttgatcgagc aggttaattg aatcttaaaa
     4021 taggcaggtc acttaatcgc gtcaaggcca aatagcgcac taattttcct acagccactg
     4081 aaggcttcta gccgattatt tggtttgagc gcacgtttgt ggcctctcgt accgccagaa
     4141 ccgcagtaat tctaccaggg attacactta tgtactccgg gctatcgtcg gcctcagggt
     4201 tgctctggaa ccaagcgagt gctttgagcc gctgaacccg atggttaacc tttttccggc
     4261 gtcgtcgtct atcaaggtgt tcagattgag aagggagatg cccatgaaaa gctcttgcga
     4321 atgcctgttt ggagtaggtt tagcgatact tgcgctgttg atattgctca ccgttaaccg
     4381 atcttgggaa acatcccatt cccccgtgag tgatagccaa tcaagcgtca ccgacctggg
     4441 cttgcgcgtc tcgatgcagc gttaggtctc gcgactcgat ttcgtgctcc gtggttttca
     4501 caagtagtgt gagctacggc atcgccggct tcacaactac gtcacccgat tccttctcag
     4561 cgatagtcac gcatctgcca aggcgtgaac acgaccacct gagtcggcgg cggtaaaacc
     4621 gatttactga aggtcgactt gttgctgttc aagtatttat tcggaaatcc cttaacgaac
     4681 atatccagca gtgcggtgtt ctcgcgatag tgcatcggga tggcgatctt gggtttgagt
     4741 tgctgtaata cttcgcgggc ggtatccggc ggcatagtga acataccgcc aatcgggatc
     4801 atggcgatgt ccaccttgcc gaaggctttg atctgttctt cggtcagttt gtgtgccaaa
     4861 tcccccaaat gggcgacgca gatggtgcct aagtcgaaga cgaatgcagc gcctttaagc
     4921 gcgttaccga actgctgttg atagatggga accgtataga ccaagacgtc tttgaccgtc
     4981 gtgctgacct tgttccactc agcgccgtaa ttgcccaggc cgcgtaaaat caccgggcca
     5041 tttttagcaa tctcgacaaa attatggttt ggatgctcgc ggccgacggt aatcgcatgg
     5101 ggcgagagct cgggtgtcgg atagaagccc ggagttaccg ggtcggtaat aattttggtg
     5161 cccctcgacg aggtgatctg aaaaaaatta tgtccgaaaa actcgatggt tacgtcgggc
     5221 tcggcggcgt aggcaggttt gaaccagctg cgcgcgacgt cgagctcacg gcaggcagaa
     5281 taagcagggc cggaatttag cgccgaaagc agaagcatga gcgcagctaa agttttgctc
     5341 atctctaacc tctagcctgc ggcgaagcgc ggaccgaact gttcggcgcg aaaaaaatca
     5401 gcgacgtatt cggtcgcggg atgacgcact agattctccg gtgtgtcggc ttgttcgaag
     5461 cgtccgtcgt gcatgaccgc gatgcggtcg gcgagggcca ccccgtcggc cagattgtgt
     5521 gtcacatata aggtcgtcag gcgattttct ttgtgaaacg ataaaatctc ttggcgcatc
     5581 ttaagtcgat tcggtggatc gatgttgctc agcggttcgt ccatgagcat tatttgcggc
     5641 gcggtgacca tcgctcggcc gagagcgact cgctgctgct ggccgccgga aagttcggag
     5701 ggtttgcgct tgaagaatgt ctcgtcaatg ccaattttcc aagccaacgg tcggaggtat
     5761 tcggcgattt tttcgctcga ccattgtcgc accttcaagg gtagcgatag attcgaatat
     5821 ttgctctctt cgaagacttt catgtgcggc cacagcgcgt aattttgaaa aatcatctgg
     5881 acgttacgtt tgcccacggg cacgtcgttg acggaaacgc cgccgataaa aatattgccg
     5941 ctatccggcg actcgagccc agctacgagc cgaagtgtgg tcgttttgcc gcagccgctc
     6001 tcaccgacga taaccatgaa ctcaccggcg ttgacatcga gattcgcttg atcgactgca
     6061 gcatggccgc cgaaatgttt ggtcagtttg cgtaaggaga gtgaaatcac agaggcatgc
     6121 tagtacaagc tcgcagacga gacaataaca gatcggagcg atgttgttgc aaatccaaga
     6181 cgctcaaaat gttcaagtgc tcgggtaagc tgctattgag agatcgagga gatggtgaca
     6241 ggggctagtc attgatgatc tccttggata tattggcgcc actcgactgc gaaatcatgg
     6301 gaggacttat ggttgggtta cttgaaccag aggcattgat tgccgatttg caacaaacgc
     6361 tgcaacagcg ccatccgcgg ccccatccga tccgccagtt gcttctaagc ggccggctca
     6421 ccaaagagca attgcaatgg tgggcacgca atcaattcca tgagtttcga aacatccacc
     6481 ggtttttcgg gatccgctat cacaagtgtc cgatcccgga gctgcgtcgc atgttgttgg
     6541 aaaacatggt cgaggaagag ggcgaagatt tgtttggcgg caagtatccg agccatcgcg
     6601 agctctgggt gcagttcgcc aacggtatcg gtgtcgcgag cgaggatatt cttaattacg
     6661 agcctttgcc cggcatccgc gccgcgttgg aaatgtacgt gtcgctggtg cagcaaagcc
     6721 attgggcggt agccatcggc accgggctgg tattcgaggg cggcggtccc aagcgcatga
     6781 aagaggagcg cgaagccatc gagaagtatt acagttgggt tcccgttgat tgcctggatt
     6841 tctttcgcgc ccacgagtac cacgacgaag gtcacggcga catggtcaca aacgtgatca
     6901 agcagcactg catggaagaa aagctgcaag ctgagatgcg cgaagcggtg cgccagcgcg
     6961 gcgatattat gtggctgcag aacgactgca tttacaatgc ctttgtccgg ccgcagttgc
     7021 ccggtgaggt tattcgggag atcgaaggca gacttgagta gtggtagtca tacggttcga
     7081 ctacccgtga tcgaccatga ctgagaagat catcgatttt cgaagcgata ctgtgaccaa
     7141 gccgtcggcc gccatgcgcc gggcgatggc ggaggcggag gtcggcgacg atgtttacat
     7201 cgaagacccg actgtcaatc gcctgcagga ccgcgctgcg gagatatttg accggcaggc
     7261 aggactgttc gtgccgtcgg gctccatggg caatctcacg tgcatcatgg cgcagaccgc
     7321 gcgcggccag gaagtcgtct gcgacgcagc gggccatatt tacaattatg agatggcttc
     7381 catggccgca ttggctggcg tattgcctcg agtgatttcg accagcgacg gcatcttgac
     7441 ctgggaggac atcgcgccgg cgattcgcga gaaaacttac acccgcccgc agacggcgct
     7501 gatcgcccta gagaacacgc acaatatggc tggtggcacg gtctatccga cgcggctggc
     7561 gcacgcgatt tgcgacaagg cccacgagtc ggggctgaaa
//
LOCUS       DATLCH010000187         7851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2000362, whole genome shotgun sequence.
ACCESSION   DATLCH010000187 DATLCH010000000
VERSION     DATLCH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000362"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VMZ02_05530"
     CDS             complement(<1..252)
                     /locus_tag="VMZ02_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71426.1"
                     /translation="MDLSKYIVKARPGMLGGKHEEKRPLRQLSALPVKRYVFINSDSH
                     PDADIYVAIHEAKDLPAQVPDYQVPHCHNTDEFYYFIGNN"
     gene            complement(526..1557)
                     /locus_tag="VMZ02_05535"
     CDS             complement(526..1557)
                     /locus_tag="VMZ02_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71427.1"
                     /translation="MQKALLIVLLLLINGSGVRAQSDFFKGKQMKIVVGASAGAASDL
                     YARVVANHLPKQIPGKPDIIVQNMPGGGSLTAANYVYSVAKPDGLTLGAVTAPIYFAQ
                     LLGRKEVQFDWAKFTWIGTPEENDELFFIRSDQPYKTLLDLRKGGEPPRCGASGVGST
                     GFYIPKLIEEIFGVRINMVTGYPGAADVDLAVEKNEIHCRGTTISAFFGREPGKTWAK
                     NGFVRFLVQTGDKRNHRLPDTPTIWELMDQEKGMDAKKRLARVVLGPGAFGRPILATP
                     GIPADRVKLLRDAYLNMLKDPEFAADAKKREWEIAPVSGERLEALAREVINQPPDIIE
                     RMKKVMGES"
     gene            complement(1566..2483)
                     /locus_tag="VMZ02_05540"
     CDS             complement(1566..2483)
                     /locus_tag="VMZ02_05540"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HUR71428.1"
                     /translation="MKPTKKLRQLLGRKNKVLAVLGAPNAFHAKIMEANGVEACFVGT
                     SITGGNYTGQPDLGILSMTECVEFAKWIARAVKLPVILDGDTGHGGIMAVRRLVRECI
                     EAGLAGLRLDDQPLDQKRRTGSAGIAIVSREDAVTRYCVAVDAKNEIDRDFVIMAQCY
                     ARNAVNGGMAELLRRLRLYETQGRVDWVQFESPHSVAEIKRARSVVKGPFSAMKGQLA
                     RALTLKEHEALGLNAAWYTFLPDLVLKTAAWSFFQDFKNKDMRAWDEFAAAHSGTPFT
                     GGGKLPWDATGMTELSKLEASYAHKAKRR"
     gene            complement(2480..3628)
                     /locus_tag="VMZ02_05545"
     CDS             complement(2480..3628)
                     /locus_tag="VMZ02_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71429.1"
                     /translation="MARNGYKIFDADTHVRPDAELLEPYMASSARVKLAQYEKYRAKN
                     KDGAVTYLMGERSYERRLGVSGDRAPQKDEYMSGYKRHQHGKPDALCERDPAVRIKDM
                     DIEGIDVNLMLPSGWFGCWTTIDDVALESAVYEAYHRWMADYCAVFPERLKGVILVSA
                     RDIKTSLAEVRRAAKESWALALFVYAPYQFPLDHPDLEPIWQAAAENDLAVALHTFTV
                     MPPYAPGGLDSWDNLWLQRSAAHPWCGQRNMAAIIGAGIMDRYPNIRVGTLEAGHGWL
                     PVWVSRLEEHTKLCPEALPPLKETIRSYVTGGRYFQSIEVSEGAAITQSVIDLLGPDI
                     LMFGSDYPHGESWFPVAAETVLGWNLQPEHLHKLLWDNAVKYYRRYSA"
     gene            complement(3879..4352)
                     /locus_tag="VMZ02_05550"
     CDS             complement(3879..4352)
                     /locus_tag="VMZ02_05550"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HUR71430.1"
                     /translation="MSTFSKKAVMFLVTGAGAGYVPRLPGTIGTLVAIPLSLALNRFA
                     LSNLLFAAVIVVGSICCAIWLSTKGALILKQKDPQVIVIDEIVGFLVANFLAPPRLTV
                     LIWSFLLFRGFDIVKVFPTKKIEQLPGGAGIVLDDVMAGVYALIGVRLLVQIGWA"
     gene            complement(4435..5805)
                     /locus_tag="VMZ02_05555"
     CDS             complement(4435..5805)
                     /locus_tag="VMZ02_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUR71431.1"
                     /translation="MKKPIQQPRNQKPVIKTAIPGPQSQELRKREDAHLAPGLQRFAL
                     MAGIVVKEARGSAVTDVDGNTLLDIIGGIGVNALGHSHPEYAHAIQAQVAKASVGSFT
                     SEPRVRLFEQLAEHRPAPSLHRTQLYSSGAEAVESALRLAKSYTGKYEFVSFSGGFHG
                     KTMGALSLMGSDFKNGYGPMVSGSHVAPYADCYRCPLKLSYDSCGLACVEATRQQIKQ
                     NSSGKVAAFLIEPMQGTAGNVIPPKEFLPAIKDVAREFGALLIVDEMITGFGRCGTYW
                     GSEHSGVEPDIVTLGKQFGAGFPISGLLSNDEIVGAKPWSDPSGSSSSYGGNPLAAAA
                     ASASLRVIDEEGLVENSKRVGAYFLKKIEDWTDRYPFVGDVRGAGLFIGMDLVRDKTT
                     KEPLATSVCQRIFHECVARGLLTMTYTHRVRIQPAMTVDEATVDSATAILTEVFDLVA
                     REGCWK"
     gene            complement(5805..6218)
                     /locus_tag="VMZ02_05560"
     CDS             complement(5805..6218)
                     /locus_tag="VMZ02_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HUR71432.1"
                     /translation="MKRRARPPLIVSLGRSQIASWIATIVDFCTLILLVEFGHVWYVA
                     ATVSGAFCGAVVNFILGRHWSFEAAREGVRGQAIRYAAVATVSLVLNSLGVYLLTEYA
                     GFHYAMSKAISALSVGILVNFPLHRRFVFRTQKYA"
     gene            complement(6360..7226)
                     /locus_tag="VMZ02_05565"
     CDS             complement(6360..7226)
                     /locus_tag="VMZ02_05565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUR71433.1"
                     /translation="MAAELSYTLAVALFIVVLSAMYGVRVAIKGRARFARVEQQGGSR
                     FLSQSAMELGYWILDPVARLLVFLGIGPNQVTWASLGLGFLSGACLSVGHFGFGAICA
                     AFSGLLDSVDGMVARISGVASDAGEVLDAAADRYGEFFFLAGLIIYYREIPVLQILAL
                     LTLLGSFMVSYSTAKAEALNVDPPKGSMRRPERAVYLTLGATLSALTIPWLESQHEFS
                     SAVGYPMVLALGFVGVVANVSAVERFRAITKAIRAREKDKAEARTRTHEKISSDDQDN
                     SQVNHHSAGIGV"
     gene            complement(7230..>7851)
                     /locus_tag="VMZ02_05570"
     CDS             complement(7230..>7851)
                     /locus_tag="VMZ02_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUR71434.1"
                     /translation="KVHSVTLNTYRDTHAKGITGWNADWRRESRYSGGGLAMDHGSHS
                     FYLTFDWLGSYPTSVKARMSNLESGKYDTEDDFTAELTFPTGIAQVHLTWTAGVRKVI
                     YVVQGEKGSITMEDDNLRIETGNASNGCDVRQPASRHVEKKSINSDWNDASHASWFNA
                     LFDEFRSAIDNGDFAGKQAQEALLCIQLIHTAYRSAREGSRELPLG"
BASE COUNT         1732 a         2297 c         2136 g         1686 t
ORIGIN      
        1 gttgttaccg ataaaatagt agaactcgtc agtattgtgg cagtgcggca cctgataatc
       61 gggaacctgc gcaggcaaat ccttggcttc gtggatggcg acgtagatgt cggcatcggg
      121 atgcgaatcg ctattgatga atacgtaacg ctttaccggc agcgcgctca attgacgcag
      181 cggccgcttc tcttcatgct tgccgccgag cataccgggc cgtgctttga cgatgtattt
      241 tgataagtcc atacaagctc ctgttcgttt acggccgtgt cgtaaagtct gtccatccga
      301 gaattcgcga ccgcagcggg atgtccgaga gctgccattt gcctccaggg gagctgtgaa
      361 agcttatgag atcgatggcg aattccggaa aatctcccct ggcccctctt ttccaaagag
      421 gggagctgcc ggccgatatt cgatatctcc gtagcatctc atccgccttt gaaaaaaggg
      481 acgacagggg atttgatttt ttcacagcct cagaggtgat cactgttacg attcgcccat
      541 aactttcttc attcgctcga tgatgtccgg cggctgattg atcacctctc tcgccaaggc
      601 ctcgagccgt tcgccgctca ccggagcaat ctcccattcc cgcttctttg cgtccgccgc
      661 gaactcagga tctttgagca tattgagata ggcgtcgcgg agcagcttca cacgatccgc
      721 agggatgccc ggagtcgcca gaatgggccg gccgaaagcg cccgggccga ggaccactcg
      781 cgccaggcgt ttcttcgcat ccatgccttt ttcttggtcc atcaattccc agatcgtcgg
      841 cgtgtcgggc aagcgatgat tgcgcttatc gccggtttgc acgagaaatc ttacgaagcc
      901 atttttcgcc caggtttttc ccggctcgcg gccaaaaaac gcgctgatgg ttgtgccacg
      961 gcaatggatc tcgtttttct cgacggctaa atccacgtcc gcggctcccg gatagccggt
     1021 caccatgttg atcctgacgc cgaagatttc ctcgatcaac ttgggtatgt aaaatcccgt
     1081 gctgccgact ccggaagcgc cgcagcgcgg cggttcgccg cccttgcgca aatcgagcag
     1141 cgtcttgtat ggctgatcgg agcggataaa aaacagctcg tcgttttctt cgggcgtgcc
     1201 aatccaggtg aacttggccc aatcgaactg aacttctttt cggccgagca gctgggcaaa
     1261 ataaatcggc gcggtcaccg cccccaaggt aagaccgtcg ggcttggcga cgctataaac
     1321 ataattagcg gctgtcagcg atccaccacc cggcatgttc tgcacgataa tgtccggttt
     1381 acccgggatc tgtttcggta ggtgattcgc caccacgcgg gcatatagat cgctcgcggc
     1441 gccggcggaa gcgccgacga cgattttcat ttgcttgcct ttgaaaaaat cgctttgggc
     1501 gcggactcca gagccattaa tcaacagcaa caagacgatc aacaatgcct tttgcatgtt
     1561 cctggctatc tccgtttcgc tttgtgtgcg taactcgctt ccaacttgct caactcggtc
     1621 atcccggttg cgtcccatgg taactttccg cctccggtaa acggcgtgcc gctgtgcgcc
     1681 gccgcgaatt catcccatgc gcgcatgtct ttgtttttga agtcttggaa aaacgaccag
     1741 gccgccgttt tgagaaccag atcgggcagg aacgtgtacc acgcggcatt gagcccgagc
     1801 gcttcatgct cttttagggt cagcgcccgc gccagctggc ctttcatcgc cgagaacggt
     1861 cctttgacga cgctgcgggc gcgtttgatc tcggccaccg agtggggcga ttcgaactgt
     1921 acccaatcga cgcggccttg ggtttcgtac aggcgcagcc gccgcaacaa ctcagccatc
     1981 ccgccgttga ccgcgttgcg cgcatagcac tgcgccatga tgacgaaatc gcgatcgatc
     2041 tcattcttgg catcgaccgc gacacaatag cgcgttacgg cgtcttcgcg gctgacaatg
     2101 gcaatccccg ccgatccggt gcgtcgtttc tggtccagcg gctggtcgtc gagccgtaaa
     2161 ccggcgaggc ccgcttcgat acattcgcgg accagacggc gcaccgccat gatgccgccg
     2221 tggccggtgt cgccgtcgag aattaccggt agcttcaccg cgcgggcgat ccacttggca
     2281 aactcgacac actcggtcat ggacaaaatg cccagatcag gttgacccgt atagttgccg
     2341 ccggtaatgc tcgtgccgac gaaacacgct tcaacgccat tggcctccat gatcttcgcg
     2401 tggaaggcat tcggcgcgcc cagcaccgcc aatactttgt ttttgcggcc aagcagttgc
     2461 cgtaacttct tggtcggctt catgcgctgt accgccggta atattttacc gcattgtccc
     2521 acaacaattt gtgcagatgt tcgggttgca agttccagcc gagcacggtt tcggcagcaa
     2581 cgggaaacca gctctcgccg tgggggtagt cggaaccgaa cataagaata tcggggccta
     2641 aaaggtctat gacgctttga gtaatcgctg caccttcgga aacttcgatg ctctggaaat
     2701 aacgaccgcc ggtaacatag ctgcgaatcg tttctttgag tggcggcaac gcttcgggac
     2761 agagcttggt gtgctcttcg agccggctca cccacaccgg cagccagcca tgtcccgctt
     2821 ccagcgtgcc cacgcgtata ttcggatagc ggtccatgat ccccgcgccg ataatggcgg
     2881 ccatattgcg ctggccgcac cagggatgcg ccgccgagcg ctgcagccag agattatccc
     2941 acgaatcgag accgcccggt gcatagggcg gcatcaccgt gaacgtgtgt agagcgacgg
     3001 ctaaatcgtt ttctgcggcg gcttgccaga ttggttcaag atcggggtga tcgagcggga
     3061 actgataggg cgcatagaca aaaagcgcaa gcgcccagct ctctttggcg gcgcggcgca
     3121 cttcggccaa ggaagtcttg atgtcgcggg ccgaaaccag gatcacgccc ttcaagcgct
     3181 caggaaagac ggcgcaatag tcggccatcc agcggtgata ggcctcgtac accgcgctct
     3241 ccagcgcaac gtcgtcgatg gtggtccaac aaccgaacca gcccgaaggc agcataagat
     3301 tgacgtcgat gccctcgatg tccatgtcct ttatgcgaac ggcgggatcg cgctcgcaaa
     3361 gcgcatcggg tttgccgtgc tgatggcgct tgtagccgct catgtattcg tctttttgcg
     3421 gtgctcgatc gccgctgacg cccagacggc gctcgtacga gcgctcgccc attaaatagg
     3481 tgaccgcgcc gtccttgttc ttggcccggt acttttcgta ctgcgccagc ttaacgcggg
     3541 cggaacttgc catgtacggt tccaacagtt cggcgtcggg cctgacatgt gtgtccgcat
     3601 cgaagatctt atagccgttg cgtgccatgg gttggctcct cgttttggtt aaagcgccaa
     3661 ccacaccgct aaccgtaaag cagcggggct gtcaagttct tttgccggct gtgtagtagc
     3721 aacatcaatc tgacaggggt taacagcaac gtaattacgt caggctgaag gatgacagcc
     3781 tgacgacgaa agaagagaaa cgaacggacg taattctaag agtatctcgt attatgccgt
     3841 agtcggatgg ctgagttcgc tctgctcgca tggagccatt aagcccagcc gatttgtact
     3901 agcagacgca cgccgatgag cgcataaaca cccgccatga cgtcgtcgag cacaatcccg
     3961 gcgccaccgg gcaattgttc gatttttttt gtcgggaata ccttgacgat atcgaagccg
     4021 cgaaacagca gaaaactcca aatcaaaacc gtcagccgcg gcggcgcaag aaaattggct
     4081 accaggaagc cgacgatttc gtcgatgaca atgacctgcg gatctttctg tttcagaata
     4141 agcgcgccct tggtggacag ccatatggcg cagcagatcg atccgacgac aatcactgcg
     4201 gcaaaaagta aattgctcag tgcgaagcgg ttaagcgcca gcgataacgg aatggcaacc
     4261 agggtgccga tggttcccgg cagccgagga acatatcctg ctccggcgcc ggtgaccaag
     4321 aacatcacgg cttttttgga aaatgtactc attaaaaata ttcgacgacg cagagaaact
     4381 ctcaagcctg ctgatcagtt ctcgctgaca ctgcctctgc aaaaggaacc ggggctactt
     4441 ccaacatcct tcgcgagcga cgagatcaaa gacttccgtc aaaatcgcgg ttgccgaatc
     4501 gactgtggct tcatcgacgg tcatggccgg ctgaatgcga actcgatgcg tgtaggtcat
     4561 ggtcaacagc ccgcgcgcga cgcactcgtg aaaaatgcgc tgacagacag aagtagcaag
     4621 cggttctttg gtcgttttgt cgcgaaccaa atccattccg atgaacaggc cggcaccgcg
     4681 cacatcaccg acgaacggat aacgatcggt ccaatcttcg atttttttca ggaagtaggc
     4741 gccgactcgc ttcgagttct cgaccagccc ttcttcatcg atgaccctga gggacgcgct
     4801 ggccgcagcg gccgccagcg gatttcctcc atagcttgaa gaagatcccg acggatcgga
     4861 ccatggctta gcgccgacga tttcatcgtt ggaaaggagc ccgctgatcg gaaacccggc
     4921 tccgaactgc ttgccgagcg taacgatatc gggctcgacg cccgagtgct ccgaacccca
     4981 gtacgtaccg caacggccga atccggtgat catctcgtcg acgatcagca gagcgccgaa
     5041 ttcccgcgcc acgtctttaa ttgccggcaa aaattccttg ggtggaatca cgtttccagc
     5101 cgttccctgc atcggctcga tcaggaaagc ggcgactttt ccgctgctgt tttgcttgat
     5161 ctgctgacga gtcgcttcga cgcacgcgag cccgcaggag tcgtagctca gcttgagcgg
     5221 acaacggtaa caatcggcgt acggcgcaac atgcgagccc gaaaccatgg ggccgtagcc
     5281 gttcttgaag tccgagccca taagcgaaag agctcccatg gtcttgccgt gaaaacctcc
     5341 gctgaaactc acgaactcgt attttcccgt gtacgatttc gccagtcgaa gtgccgattc
     5401 aacggcttca gcgccgctcg aatacagctg ggtccggtgc agcgaaggcg ccggacgatg
     5461 ctctgccagc tgttcgaaca acctgaccct gggttcgctg gtaaaactgc cgaccgaagc
     5521 ttttgccacc tgggcttgaa tcgcgtgcgc gtattccggg tgactatgac ccagcgcgtt
     5581 gacgccgatg ccgccgatga tatcgagcaa agtattgccg tcgacgtcag tcaccgcgct
     5641 gccgcgggcc tccttaacga cgatgcccgc catcaaggca aagcgctgca gtcccggagc
     5701 taaatgagca tcttcgcgtt tgcgcagctc ctgactttgc ggccccggaa tagccgtctt
     5761 gatgaccggc ttttgattcc ttggctgctg aatcggcttt ttcatcaggc gtatttctgt
     5821 gtgcgaaaga caaaccggcg atgaagcgga aaattaacta gaattccgac cgaaagagcg
     5881 ctgattgcct ttgacatcgc gtagtgaaac ccggcgtact cggtcagcaa ataaacgccc
     5941 aggctattca gaaccagact caccgtcgca accgccgcat aacgaatcgc ttggccgcga
     6001 acgccctcac gagccgcctc gaaactccaa tgccggccta agataaaatt gacgacggcg
     6061 ccacaaaaag ccccgctgac agtcgctgcg acataccaca catggccgaa ctcgactaac
     6121 aaaatcagcg tacagaagtc gacaatcgtc gcgatccacg atgcgatttg ggagcgtccc
     6181 agcgaaacta tcaatggggg acgggcgcgt cgtttcacag ttgcaagcct ctaacagggt
     6241 gctgaaaccc ctcgaagtcc ttcgacgagc tcaaacgaac ggaataggag ttaaaatcgt
     6301 tgaccaaaat ccgttcatgc tgaagctctc gaagcatgag tacctttttt taccacctgc
     6361 tagaccccga tgccggccga gtgatggttg acttgagagt tgtcttggtc atcgctcgag
     6421 attttttcat gcgttctagt gcgcgcctca gccttatctt tttcgcgggc tcgaatggct
     6481 tttgtgatcg cccgaaaccg ctcgacggca gagacattgg ccaccacgcc aacaaatccc
     6541 agcgccagca ccattggata accgacggcg ctggaaaact cgtgctggct ttccagccag
     6601 ggaatggtca gcgcagataa ggttgctccg agggtgagat atacggcgcg ctcagggcgc
     6661 cgcatgcttc cttttggcgg atctacattg agcgcctcgg ccttggccgt cgaataactc
     6721 accatgaagc tgccaagtag ggtgagcagc gcaagaatct gcagcacggg aatttcccgg
     6781 taatagatga ttaaacccgc taaaaaaaag aattcgccgt agcggtccgc cgcggcatcg
     6841 agaacctcgc cggcatcgga ggcgacaccg gaaatgcgtg caaccattcc atcgacggaa
     6901 tccagcaggc cggaaaaagc ggcgcatatc gcgccgaaac caaagtggcc gacactcaag
     6961 caagcgccgg ataaaaaccc cagcccgagt gaagcccagg tgacttgatt gggacctatg
     7021 ccgagaaaaa ccaggagcct cgcaactggg tcaagaatcc aataacccaa ttccatggca
     7081 ctctgactca gaaaccggct gccgccctgt tgctcgaccc gggcgaaacg agcgcgcccc
     7141 ttgatcgcga cccgcactcc atacattgcc gaaagcacga caatgaacag cgcgaccgcc
     7201 agggtatagc tgagttcagc ggccattttt catcccagcg gcagctcccg agagccctcg
     7261 cgcgcggatc ggtaagcggt atgaatcaat tggatacaca gcaaagcttc ctgcgcctgt
     7321 ttgccggcga aatcaccgtt gtcgatggcg ctgcggaatt cgtcaaaaag ggcattaaac
     7381 cagcttgcat ggctggcatc gttccaatcg gagttaatcg attttttttc gacatgccgg
     7441 ctcgccggct ggcgtacgtc gcagccattg ctggcgttgc cagtctcgat gcgcagatta
     7501 tcatcctcca tcgtgatcga gcctttttcc ccctgaacga cgtaaataac tttccgcact
     7561 ccagcggtcc aggtgagatg cacttgagcg ataccggtgg gaaacgtcaa ctcagctgtg
     7621 aagtcgtctt cggtgtcata ttttccagat tcgagattgc tcatcctggc ttttacggaa
     7681 gtaggatagg agccgagcca gtcaaacgtc aaataaaaac tatgactgcc gtgatccatg
     7741 gcgagacctc cgccactgta gcggctttcg cgccgccaat cggcgttcca tccggtaatc
     7801 cccttggcgt gggtgtcccg gtaggtattt aatgtcaccg aatgtacttt c
//
LOCUS       DATLCH010000188         4946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4217242, whole genome shotgun sequence.
ACCESSION   DATLCH010000188 DATLCH010000000
VERSION     DATLCH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4217242"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1368
                     /locus_tag="VMZ02_05575"
     CDS             343..1368
                     /locus_tag="VMZ02_05575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71435.1"
                     /translation="MQKLKTTFGLTLSNRALVTKGTPARELIDMAVQAENTAAIDAIW
                     VGDSILSKPRLECIPLLGAIAAHTSRVKLGVACMATIAQRNPMLLALQWASLDVLSGG
                     RTWLAACMGYPASQHPMAAKELEVMGIQSKERPGRLVEMLQALRLLWSDEHATFHGKY
                     YSFNDVNLEPKPEQHPCPIYIAATPRATQIGEHGVERSLRRMARYADGWMSNQIELSM
                     FQDYQGRLRKMLIEEGRDPYAFKTALYYGVAINRDRDQAFREAKAFLDAYYQKDFTRQ
                     GVEIWTACGPVEHCVDCLRGFIDAGVDHVAIRPIGDDLNDQFHIFLRELIPALSRLAG
                     SVSQTTV"
     gene            complement(1441..1974)
                     /locus_tag="VMZ02_05580"
     CDS             complement(1441..1974)
                     /locus_tag="VMZ02_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HUR71436.1"
                     /translation="MEAAGLALDALGRVREMVRDALKDLTPDEMLSPPKPHIAWLTWH
                     IARVQDANFSGLLDRPQLWITDGWHQRFNMPPESRDYGSGHRQTQEQVDAFTITDKSL
                     LLAYHDAVFAQTKDYLSTVTNGDLNRVLNEPQYSPLPTLSIRLASVINCNTRHAGQIE
                     YLRGFVKFKGWFPGAEK"
     gene            2276..3247
                     /locus_tag="VMZ02_05585"
     CDS             2276..3247
                     /locus_tag="VMZ02_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71437.1"
                     /translation="MTNKTKAIYCVLAISITCSVQRLHAVDSAPQRLRILYASTATSF
                     SPIWIATDLGIYKKHGLDAEAVLLGGGPLVASVLLSGSADIGVGAGDAPVRVTLQGGD
                     MKIFALQKHARILSIVSKPSIQDLSQIKVLGIQSLGSSTHNFWLWYARQRKLAEHQVQ
                     FVHTSSSAENFIALKSGRIDAGVFGPPFDVQAERDGFRLLVEGIKNPQPFPATAYFAM
                     SAFLTKRPEVARRFIEAMKEAVQVFREDKTTAFKVITRRLKLTDINVLDRAYDYERPF
                     MRRDLSLQIDVIDNALAEAKRVLGANAKRPELTIDDVIDKTFVGVAR"
     gene            complement(3359..4474)
                     /locus_tag="VMZ02_05590"
     CDS             complement(3359..4474)
                     /locus_tag="VMZ02_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71438.1"
                     /translation="MGITSLMQTFAAVLVLTAIDLAFAQPNWQAEWDRTVRAAEAEGQ
                     LTLYGCCYEYDRVIEGFKKKYPKLKIATVLSGGGQLATRILAERRGDKYLVDVVSSGA
                     NTLHDALYKAHALDPIKPVLILPEVVDQSKWYEGEHRYIDPERRYIFVFNANSQSGQI
                     TYNLKQVNPGEFKSHWDLVTTKWRGKVLSLDPTIFGMGTTLQFFYHHPDLGPPFIKKL
                     YGEMQVTVGRDPRQLTDWLSTGKFLLCIRCNAGSEVGKAVQQQLPIGYLDTDEWKEGG
                     STSAAGGTLGIPTRAPHPNAAKLFVNWLLSREGQIALQKLGRPDAHNSRRIDIPKDDV
                     DPTNRMDPGKKYFDLAKPEYQDLTPIFKLIKEVLPQK"
     gene            complement(4538..>4946)
                     /locus_tag="VMZ02_05595"
     CDS             complement(4538..>4946)
                     /locus_tag="VMZ02_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HUR71439.1"
                     /translation="GIGGGLGMKLAHPEKTFIVTAGDGTYMFNNPTACHFVSEAYDLP
                     LLTIICNNAIWHSTKAATQQVLPDGWAVSTGNFPLTALAPSPRYEKVVEAHGGYGEMV
                     DDPQEVLPALKRALKVVKEEKRQAVLNVICGSH"
BASE COUNT         1095 a         1407 c         1342 g         1102 t
ORIGIN      
        1 tttgaaaaag agcgaagccg gcgtaagcaa catcgattgg cgagcgagca aggtggactc
       61 acagccgaag tgaatcaccg gcgagacgag ctctgagcgg aacgaagtag gtcgtcgttc
      121 gctacatcgc acgcgactcg aaagtagctt tggcctgctc actgaggcga acccagccag
      181 gcttgtcagt agcagcaatt tattctgagc cgcgggaaac cagccagcgt aactcaccaa
      241 ccgcgaagat aatcgatatg gcaggggtgg ggcgtattgc gataatcgcc tcgattagtt
      301 cacacttgaa aaaattcgcg ccgccgtcct atggtcgccg ccatgcaaaa actcaaaact
      361 actttcggtc ttaccttgtc caaccgggcg ctggtcacca agggaacgcc ggcgcgggaa
      421 ctgatcgaca tggccgtcca ggccgagaac accgccgcca tcgacgcgat ctgggtcgga
      481 gatagcattc tgagcaagcc gcggctcgaa tgcattccgc tcttgggcgc catcgccgcg
      541 catacatcgc gagtgaaact gggcgtcgcc tgcatggcga ccatcgccca gcgcaatccc
      601 atgctgctcg ctttacagtg ggccagtctc gatgtgttgt cgggcggacg gacttggctg
      661 gcggcctgca tgggttatcc ggcgagccag catccgatgg cggccaagga actcgaagtc
      721 atgggcatcc agagcaagga gcggcccggg cgactggtcg agatgcttca ggcactacgt
      781 cttctgtggt ctgacgaaca tgcgaccttt cacggcaaat attactcttt caatgacgtg
      841 aacctcgagc caaagccgga gcagcatccc tgcccgatct acattgcggc aacaccgcgt
      901 gccacacaaa tcggcgagca tggcgtggag cgctcgctac gccgcatggc ccgctatgcc
      961 gacggttgga tgagcaatca gatcgagctt tcgatgtttc aagattatca gggacggctg
     1021 cgaaagatgc tgatcgaaga agggcgcgat ccttatgcct tcaaaactgc gctgtattac
     1081 ggagtggcga taaaccgcga tcgcgatcaa gcctttcgcg aagcaaaagc ttttctcgac
     1141 gcctactatc aaaaggactt cacccgtcaa ggtgtggaaa tctggaccgc ttgcggtccg
     1201 gtggagcact gcgtcgactg tctgcgcggc ttcatcgatg ccggcgtaga tcatgtcgcc
     1261 atccgtccga ttggcgacga cctgaacgac caattccaca tttttttacg agagctgata
     1321 cccgcgttga gtcgacttgc cggcagcgtc tcgcagacga cagtttagtg aagtgcacgg
     1381 tgcgatcgcg cgatcccgcg ctcgatgata aaaagacacc gccgcattag tggcgcgacg
     1441 ttatttctct gctccaggaa accagccttt gaacttaacg aacccgcgca gatattcgat
     1501 ctggccggca tgacgggtgt tgcagttgat gacacttgcg agccggatac tcaacgtcgg
     1561 cagcggcgag tattgcggtt cgttgaggac gcgattcaaa tcgccgttgg tgaccgttga
     1621 caggtaatct ttggtctgag cgaacacggc gtcgtggtat gcgagcagca gcgacttgtc
     1681 ggtgatggta aacgcatcca cctgctcttg ggtctgccgg tgaccgctgc cgtagtctcg
     1741 tgattccggc ggcatattga agcgctggtg ccaaccgtcc gttatccaca actgcggccg
     1801 atcgagcaat cccgagaagt tggcgtcttg cacccgtgcg atatgccagg tcaaccaggc
     1861 gatatgtggc ttgggtggcg acaacatttc gtccggcgtc aaatccttca acgcatcgcg
     1921 gaccatttcg cgcacccggc ctaacgcatc cagtgccaat ccagctgcct ccataaattc
     1981 ctccgtaatt attcacaccg tatcgtaggc ttcattcgct gtaagcaccg cgccacgact
     2041 atatcaacac ttcggggttc cattgaagaa ctatttccca cagagtcacg gagtccatag
     2101 tgttcggatc aacggctgat cgagagttct ttgctccacg atctcggcga ctctgcggtg
     2161 cgaattccgc tgtctgcttc acaggaatcc atgaactgca ccatgaactc gcccagcact
     2221 tgtaagttgg cgcatcctcg tcttatggtc cccccaatat ccggagaaaa agcgcatgac
     2281 caataaaacc aaagccatct actgcgttct cgcgatatcg attacttgtt ccgtccaaag
     2341 acttcatgcc gttgatagcg cgcctcagcg actgcggatt ctttacgcct cgacggcaac
     2401 atcattctcg ccgatctgga tcgccaccga tttgggtatt tataaaaaac acggcctcga
     2461 cgccgaagcg gtgctgctcg gcggcggtcc gctggtggca tcggtgctgc tctcgggctc
     2521 cgccgatatc ggcgtcggcg ccggcgacgc cccagtccgc gtcactcttc agggcggcga
     2581 catgaaaatc ttcgccctgc aaaaacacgc gcgcatattg agcatcgtca gcaaaccgtc
     2641 aattcaggat ttgtcgcaaa tcaaagtgct gggaattcaa agcctcggca gcagcacgca
     2701 caacttttgg ctgtggtatg cccggcaaag aaagctcgcc gaacatcaag tccaattcgt
     2761 ccacaccagt tcgtcggcag agaactttat cgccctgaaa tccggccgca tcgacgccgg
     2821 cgtctttggc ccgcccttcg acgtgcaagc ggagcgcgac ggctttcgtc tgctggtcga
     2881 aggtatcaag aatccgcagc catttccggc gaccgcctat ttcgcgatgt cagcattttt
     2941 aactaaacgc cccgaggtgg cacgccgttt tatcgaagcg atgaaggaag ccgtgcaagt
     3001 atttcgtgag gacaaaacga cggcatttaa agttattaca agacggctga aattaaccga
     3061 catcaacgtg cttgaccgcg cctatgacta cgaaaggcca ttcatgcgcc gcgatctctc
     3121 gctccagatt gacgttatcg acaatgcgct ggcagaggcc aaacgcgtgc ttggcgccaa
     3181 tgccaaacgc cccgaattaa cgatcgacga cgtgatcgac aaaacttttg tcggcgtggc
     3241 gcgctgaaac ggacgaactc gtggtcactt tcattccgcg gtttcgaatc cgaacaagtg
     3301 ttttggaagt tcgcctatac ttaaaacgag aacagaggat ttctagcggc tcaaccatct
     3361 atttctgggg caagacttcc ttgatcagct tgaagatcgg cgtcaaatct tggtactcgg
     3421 gtttggcgag atcaaaatat tttttacctg gatccatccg attggttggg tcgacgtcat
     3481 ccttcggtat gtcgatgcgt cgcgaattgt gcgcgtcggg gcggcccaat ttttgtagcg
     3541 cgatctgtcc ttcacgcgac agcagccagt tgacaaagag tttggccgcg ttcggatgcg
     3601 gagcgcgcgt tggaatgccg agtgtgccgc cggccgcgct ggtgctgccg ccctctttcc
     3661 attcatccgt atcgagataa ccgatcggca attgctgctg cacggctttg cccacttcgc
     3721 tgccggcgtt gcagcggatg cagagtaaaa actttcctgt cgacagccag tcggtcaact
     3781 gacgcgggtc gcgccctaca gtaacttgca tctcgccata aagtttcttg ataaaagggg
     3841 ggccaagatc agggtgatgg taaaaaaatt gtagcgtggt acccatgccg aaaatcgtcg
     3901 gatcgaggga gagcaccttg cccctccatt tcgtcgtcac cagatcccag tgcgatttga
     3961 attcgccagg attcacctgc ttgagattgt aagtaatttg gccggactgc gagttggcat
     4021 taaaaacgaa aatgtaacgt ctttcagggt ctatgtaccg gtgctcgcct tcgtaccatt
     4081 tcgattgatc gacaacctcg ggaagaatga gcacaggctt gatcgggtcg agcgcgtgcg
     4141 ccttgtagag cgcgtcgtgc aacgtattgg cgcccgagct gacaacgtca acgagatact
     4201 tgtcgccgcg ccgttcggcg agaatccgcg tcgccagttg gccgcccccg cttagcaccg
     4261 tcgcgatctt gagcttggga tacttctttt taaaaccttc gataacgcgg tcgtattcgt
     4321 agcagcaacc gtacaacgtc aattgccctt ccgcttcggc ggcgcgcacc gttctgtccc
     4381 actccgcctg ccaatttggc tgcgcgaaag ctaagtcaat cgctgtcaaa accaagacag
     4441 cggcaaatgt ttgcataagc gaagtgatac ccatggagtc cctaggtgat caaacttcaa
     4501 aatgccactc ggagttaacc gaacctaaac ggtttttcta gtggctgccg cagatcacat
     4561 tcagcaccgc ttggcgcttt tcttctttga cgacttttag cgcgcgtttg agcgccggca
     4621 gcacttcctg cggatcgtcg accatctcgc cgtagccgcc gtgagcctcg acgaccttct
     4681 cgtaacgcgg cgacggcgct aatgcggtca agggaaaatt tcccgtgctc accgcccaac
     4741 cgtccggtaa gacttgttgc gttgccgctt tcgtcgagtg ccagatggcg ttgttacaga
     4801 taattgtcaa cagcggcagg tcgtaggctt ccgaaacgaa atggcaggcc gtgggattgt
     4861 tgaacatata agttccatcg ccggcggtga cgatgaaagt tttttccgga tgcgccagtt
     4921 tcatgcccaa gccaccgcca atgccc
//
LOCUS       DATLCH010000189         3731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4222576, whole genome shotgun sequence.
ACCESSION   DATLCH010000189 DATLCH010000000
VERSION     DATLCH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4222576"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..646)
                     /locus_tag="VMZ02_05600"
     CDS             complement(308..646)
                     /locus_tag="VMZ02_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71440.1"
                     /translation="MVSQADRETEIQRLAGEIGRLIRESDPETREELAHTADILLREE
                     AMRPNFVTAGNKPIANPQERPLNPLAAGIALMLIGGAIALLLPLVGLALAACGALSAL
                     WGIAISARRN"
     gene            1101..2429
                     /locus_tag="VMZ02_05605"
     CDS             1101..2429
                     /locus_tag="VMZ02_05605"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HUR71441.1"
                     /translation="MLQVDEQQGRSVEAKRAGTDLPRVVIVGGGFGGLCAAQSMRDLP
                     VQITLIDRRNFHLFQPLLYQVATGWLSPANIASTLRAVLRAQKNVRVLLAEVTDVDAE
                     RRTVIFSGGEVGYDTLIVASGSSHHYFGHDEWEALAPGLKTIEDATEMRRRIFLAFES
                     AERESDPAKIRAWLTFVIVGGGPTGVELAGALADLANQTLTHDFRSIDPSAAQILLLE
                     ASDRILSTYPEELSTKAQTFLHGLGVALQLRTSVTELHAGEVIVQRNDQREVIASHTV
                     LWAAGVRASQLGKALARSTGAALDRSGRVMVQGDFTIANHPEIFVIGDLAGYAMPMGK
                     TLPGIAPVAQQAGRYVAKVIGARLNRKSYRSFNYRDRGSMAIVGRATAIADLRGVHLA
                     GFTAWLAWLFVHLMQLVEFENRVLVLIQWGWFYFRRSRAARLITGDRFPL"
     gene            2764..3510
                     /locus_tag="VMZ02_05610"
     CDS             2764..3510
                     /locus_tag="VMZ02_05610"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUR71442.1"
                     /translation="MEDGNLRPNRNDGKKNLPRRSLGLNWQVLERLGSMLLGSALMLY
                     GAKRRGILPTLLGGLFLYRGAAGHGAFGLPGLQSNKRPHPQTSVPHGEGTKVTSAITI
                     DRPASELYHYWRDLTHLPRFMKRLESVQSTDGGRSHWVLNSAAGVKLSWDAEIINDVP
                     NELLAWRSLPGGDLDHAGSVRFEPAAGGRGTTVKVTVEYRPPVGKLGVVAASLLGVEP
                     ERLIKTDLSRLKQLVETGEISSVEGQPHGF"
     gene            complement(3507..>3731)
                     /locus_tag="VMZ02_05615"
     CDS             complement(3507..>3731)
                     /locus_tag="VMZ02_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71443.1"
                     /translation="GGLPPLEISPNRPVATPIPSSGISATRSELEVKGNCFAIYFLWL
                     SVRLTESSPGPVTVARYKMIDHRFIQNFFI"
BASE COUNT          805 a         1046 c         1063 g          817 t
ORIGIN      
        1 gatgttgctt aacacccgct gttgcggcac agcagcacaa ccgtgaacac gcccgctggt
       61 taaaccgagt cgagcacaaa cgtgatcttt aaatcgaggc gataggcaac tatcttatct
      121 tccccacctg ttgttctttg acgcaagcat ctctgttgcg gtttcgatgc cttgagcaat
      181 tgcatcttca acgctcattc gattaacgct cattcgattc gctggaacct tcatgatttc
      241 cacgatcttt gtgacagatc gcggagcaat gggccgggcg agaagtttct agtatctgct
      301 tgagcggtta gtttcttcgg gcgctgatgg cgatgcccca gagcgccgat aacgcgccgc
      361 aggctgccag cgccaatccg accaacggca gcaatagtgc gatcgcgccg ccgattaaca
      421 tcagggcgat accggcggcg agaggattga gcgggcgctc ctgtgggttg gcgatcggct
      481 tattacccgc ggttacaaaa ttcggtcgca tggcttcctc gcgcaagaga atatcggcgg
      541 tgtgcgcgag ctcttcgcgt gtttcgggat ccgactcgcg aattaaccgg ccaatttcac
      601 cggcgagccg ttggatctcg gtttcacgat cggcttgcga aaccacgggt gctcgggccg
      661 ctgcggacgg agcctcattg tcagtcgggt gctgatccat gttgtgtgaa aaaccagcaa
      721 tggttatgcc actcggcctg cactcgcgcc tgcaagatcg caacgcgtat tccaagtgga
      781 ttgagatttg caaactcctg tggcgactaa tactccccaa ggtaggtacc ccggtgaaac
      841 taagtaaaat ctcccgacaa tcacgcggat taaacaaact tgtagaaacg gttttgatcg
      901 agctcagtcc gacagctaaa gcaaatctag aaaagctcca gtcgcattcc ttggacaagc
      961 acggacgcca acccgctgct tcccaaataa tcgaacggct gataaacgct gctgcggtga
     1021 acgtcccgga cgcgccttat ccgtcttgac agagatgccg atgtgtcatg cgtcgcggat
     1081 taccggtgta aatctctgtc atgcttcaag ttgacgagca gcagggccga agtgtagagg
     1141 caaagcgcgc cggcactgat ctgcctcgcg tggtgatcgt cggcggcggc ttcggtggac
     1201 tgtgcgcggc gcagtccatg agagatttgc cggtccaaat cactttgatc gaccggcgca
     1261 atttccatct atttcaaccg ctgctatatc aggtcgcgac cgggtggctt tcgccggcta
     1321 acattgcctc cacgctccgt gcggttttgc gcgcgcaaaa aaatgtccgg gttctgctcg
     1381 ccgaggtgac cgacgtcgac gctgagcggc gcacggtaat cttctctggc ggcgaggtcg
     1441 gttacgatac tttgatcgtt gccagcggat cgagccatca ttatttcgga cacgacgaat
     1501 gggaagcgct ggcgcctggc ctcaagacca ttgaagacgc aacggaaatg cggcggcgca
     1561 tttttttggc ttttgaatcg gcggagcgcg agagtgatcc ggcgaagatt cgcgcctggc
     1621 tcaccttcgt cattgtcggc ggtggaccga ctggagtgga actcgccgga gcgctggcag
     1681 acctcgccaa ccaaactctg actcacgatt tccgcagcat cgatccgagc gctgcgcaga
     1741 ttcttttgct cgaagcctct gaccgcatat tgtcgacgta tccagaggag ctttcgacca
     1801 aagcgcagac ttttttgcat gggcttgggg ttgctctaca actgcgcacg tcagtcaccg
     1861 aactacatgc gggggaagtc atcgtgcagc gcaatgatca gcgcgaagtg atcgcatcgc
     1921 acaccgtcct atgggccgcc ggcgtgcgtg cctcccaact cgggaaagcg ctggcacgct
     1981 cgaccggcgc agcgcttgat cgcagcggcc gagtgatggt tcaaggcgat tttaccattg
     2041 ccaatcatcc ggagattttc gtcataggcg atctggccgg ttatgcgatg ccgatgggca
     2101 aaaccttgcc gggcatcgct cccgtagccc agcaggcggg acgttatgtc gccaaagtta
     2161 ttggcgcacg cctgaatcgt aaaagttacc ggtcgttcaa ttaccgagac cgcggcagca
     2221 tggcgatcgt cgggcgggca acggccattg ccgacttacg cggagttcat ctcgccggtt
     2281 ttaccgcttg gctggcttgg ctgttcgtcc atttgatgca actggtcgaa ttcgaaaacc
     2341 gtgtgcttgt cctgattcaa tggggctggt tttattttcg ccgcagccgg gcggcgcgtt
     2401 tgataacggg tgacaggttt cctttgtaat cccatcccaa tcaaaaccat tgcatcgaca
     2461 tagagatgta ccgatgctgc gacgccgacg cgccaaatag cgcaaatccg ggacgaatgg
     2521 tcggcgccgc tcaggtcgaa gctccttcgc gagcccaaat cggctggtat attccttgcg
     2581 cagtgcatgt cccggctgaa gaatcataag cgcgggcacg aagcaacgtg gacccgacac
     2641 gatgaacgct ctggctactt ttcagcagcg tgagttcttg agcgccgccc cgcagcggca
     2701 aaatatccgg cgacaaacaa acttcaattg atcgtttggc gctggtttaa cggaggacgc
     2761 acaatggaag acggcaatct ccgcccgaac cggaacgatg gaaaaaaaaa tctcccgcgg
     2821 cgttcattgg gcttgaattg gcaggtgctt gagcggctcg gatcgatgct tctcggcagc
     2881 gccctgatgc tctacggcgc caagcgacgc ggtatccttc caacgctgtt ggggggttta
     2941 ttcctttacc gcggcgcagc cggacatggg gcgttcggat tgccagggtt gcagtccaac
     3001 aaacggcccc atccgcaaac cagcgtcccc catggcgaag gaacgaaagt cactagtgca
     3061 attaccatcg atcgaccggc aagcgagctg tatcattatt ggcgcgatct aacccatttg
     3121 ccgagattca tgaagcggct ggagtcggta caaagcaccg atggcggacg ctcgcactgg
     3181 gtattgaatt ccgccgccgg cgtcaagctt tcctgggatg ccgagataat caacgacgtg
     3241 ccaaacgagt tactcgcctg gcgttcgctt cccggaggag atctcgatca cgccggttcc
     3301 gtccgattcg aacccgctgc aggcggccga ggtaccacgg tgaaagtgac cgtcgaatac
     3361 cggcctccgg tcggcaaatt gggcgtggtt gcggcgagtc ttctgggcgt cgaaccggag
     3421 cggctcatca aaaccgacct gagccgtctg aaacagttag tcgaaaccgg cgagatatct
     3481 tccgttgaag gtcagccaca cggcttctaa ataaaaaaat tctgaatgaa acgatgatct
     3541 atcattttat atcgcgcaac cgttaccggc ccaggcgagg attccgtcag acgcacgctc
     3601 agccatagaa aataaatggc aaaacaattt cccttaactt cgagttcgct ccttgttgcg
     3661 ctgattccgc tactgggtat cggcgtagca acggggcggt taggggaaat ttctagcggt
     3721 ggcagcccgc c
//
LOCUS       DATLCH010000190         3462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2757260, whole genome shotgun sequence.
ACCESSION   DATLCH010000190 DATLCH010000000
VERSION     DATLCH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757260"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VMZ02_05620"
     CDS             complement(<1..652)
                     /locus_tag="VMZ02_05620"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR71444.1"
                     /translation="MPPLIAVQQVSRIFLTGTRTITALDNVSCDIQSGNFVSIVGPSG
                     CGKSTLLKIISGLMPPSSGGVMVAGRQVDSPLQNVGMVFQAPVLLKWRSVLGNVLLPV
                     EFAKLNAANFTDKAHALIKLVGLEGFEEMYPHELSGGMQQRASLCRALVTDPQLLLMD
                     EPFGALDAMTRDELDIELMRIWEERKKTVLFVTHSIQEAVFLSDLVFVMSARPGRLL"
     gene            complement(678..1676)
                     /locus_tag="VMZ02_05625"
     CDS             complement(678..1676)
                     /locus_tag="VMZ02_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71445.1"
                     /translation="MKFILRISLSAVLTAALAGAGILQGAELTPANIRMDFIIGGKHA
                     PWYVALEKGFYAKRGLAATILASTGSADTVRTIGSGGADFGFADAATMIVAKSRGTPV
                     QAAAQFGYVGATILWRDEGVIKGIKDLPGKSLAISPGQAQWYLMPAYARINKIDFKAI
                     KIQETAAPIQPAALATKKADFIIMFRASNDEVAEQAAAKVGVKLTRVFMKDTGLDIYG
                     TSLIVKDEDVKKRPEFVRAYVEATLEGLRYARDNQEEALKILLKHKPELDSLLTTQQL
                     KNALTEVFLPLESVNVGMGFMKPEIMEKTVKVVNEYFDVGRKVAAKEVYTNQFIKR"
     gene            complement(1700..2476)
                     /locus_tag="VMZ02_05630"
     CDS             complement(1700..2476)
                     /locus_tag="VMZ02_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR71446.1"
                     /translation="MKTSWKNIAEPVIFFIVLLILWEILVEVIKVPALILPPPRDLYS
                     AFIKKLPILGHHSLITFIEAFGGFALSLVLGVGFAISVVYSRHLQNTIYPLIVILYAM
                     PKSAFAPLMVIWVGYGLFSKISIAFLVAFFPIVVNTVVGLKEVEPELLELARINRGSA
                     WDVFKKIRLPNSLPYMFAGIKVAIVLSVTGAIVAEFVAANEGLGYLVLQANYSLDTAF
                     ALVILLILAVLSLGLFWLAELCQRKLAPWSAAVREVEGSF"
     gene            2647..>3462
                     /locus_tag="VMZ02_05635"
     CDS             2647..>3462
                     /locus_tag="VMZ02_05635"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUR71447.1"
                     /translation="MAQITFTPGELYDAWPVLSQPERIEGFEFLRRDDAESFFLQLNL
                     RERTRLVLGLPPNERRLWMRLLAPDDAADLIQEAPANQRTALLELLDAATQREVKGLL
                     DYAEDDAGGLMNTRYSRLRPEMTVGEAISYLRRDAQARTRTTYYAYVVDHEERLLGVI
                     TFRDLIVAPADKSVREIMRTQIISANEEMDQEALSKLFMRHHLLMIPIVDDAGHIKGI
                     VSVDDIVDVVQEEASEDIQKVGGMEALDAPYLQIGFWRMVKKRAGWLAILFVGE"
BASE COUNT          854 a          967 c          916 g          725 t
ORIGIN      
        1 ccaatagacg gcctggccgg gccgacataa cgaaaaccaa gtccgaaaga aacaccgctt
       61 cctgaatgct gtgcgtgacg aaaagaaccg tttttttgcg ttcttcccaa atgcgcatca
      121 gctcgatatc gagctcgtcg cgagtcatgg cgtcaagcgc accaaacggt tcgtccatca
      181 acaaaagctg cggatcggtg accaatgcac ggcaaagtga cgcgcgttgc tgcatgccgc
      241 cggaaagctc gtgcggatac atctcttcga acccttccaa accgacgagt ttgatcaagg
      301 catgcgcctt gtcggtaaaa ttagcagcgt tgagcttggc gaactccacc ggcaacaaca
      361 cattgccgag aaccgagcgc catttgagca gcaccggcgc ttggaacacc atgccgacat
      421 tttgcagcgg gctgtcgacc tgacggcccg ccaccatgac gccgcccgat gatggcggca
      481 tcaagcccga gatgattttc aacaaagtgc ttttgccgca gccgctcggc cctacgatag
      541 aaacgaaatt acccgattga atgtcgcaag agacattatc caacgccgta attgtccgag
      601 taccggtaag aaaaatccgc gaaacttgtt ggactgcgat cagcggcggc acgtaaagac
      661 tttcggctgg gcggcgctta acgcttgatg aattgattgg tgtagacttc tttggccgcc
      721 accttgcggc cgacgtcaaa gtactcgttg accaccttga cggttttctc catgatctcc
      781 ggcttcataa agcccatgcc cacattgacc gattccagcg gcaaaaacac ctccgttagc
      841 gcgttcttga gctgctgcgt ggtcagcaag gaatcgagct cgggtttatg cttgagcagg
      901 attttcaacg cctcttcctg attgtcgcgc gcgtaacgca atccttccaa agttgcctca
      961 acataggcac ggacaaattc cggacgcttc ttaacgtcct catctttgac gatcaaactc
     1021 gtgccgtaaa tatccagacc ggtatctttc atgaacacgc gagtaagctt gacgccaacc
     1081 tttgccgccg cctgttcggc aacctcatca ttggaagccc ggaacatgat gatgaagtcg
     1141 gcttttttag tcgccaatgc ggccggctga atcggcgccg cggtttcctg aatcttgatc
     1201 gccttgaaat caattttatt gatgcgggcg taagccggca tcagatacca ctgcgcctga
     1261 ccggggctta tcgccagcga cttgccgggt agatccttta tgcctttgat cacgccctca
     1321 tcgcgccata agatagtcgc accgacatat ccgaactgcg cggcagcttg caccggcgta
     1381 ccgcgcgatt tggcaacgat catggttgcg gcatcggcaa agccaaagtc ggcgccgccg
     1441 gaaccgatcg tgcggacggt atccgcagaa ccggtgcttg ccagaattgt cgctgccaag
     1501 ccgcgcttgg cgtaaaaacc tttttccagt gcgacatacc acggcgcgtg cttgccgccg
     1561 ataatgaaat ccatgcgaat gtttgccggt gtcagttcag cgccctgcaa tattcccgcg
     1621 cccgctaacg ctgcggttaa gaccgccgat agcgatattc taagaataaa tttcatgagt
     1681 gtcctccaca gacgagtaat cagaacgaac cttcaacttc gcgcaccgca gcgctccagg
     1741 gcgcgagctt tctttggcag agttccgcca accaaaagag gcccaaggac aacactgcga
     1801 gaatcagcaa aatcaccaac gcaaacgcgg tgtccaaact ataattagct tggagcacca
     1861 gatagcctaa tccttcgtta gcagcgacga actcggcgac gatggcaccg gtgaccgaga
     1921 gcacgatggc gactttgatg ccggcgaaca tgtacggcag cgagttaggc aagcgaatct
     1981 ttttgaagac gtcccacgcc gagccgcggt tgatacgtgc cagctccaac agttcgggct
     2041 cgacttcttt cagtccgacc accgtattca caacgattgg aaaaaacgcc actaaaaagg
     2101 caatggaaat tttcgagaaa agaccataac cgacccagat caccatcagt ggcgcaaagg
     2161 cgctcttggg catggcgtag aggattacga tcaacggata gatggtgttc tgcaagtggc
     2221 gtgaatagac cactgagatg gcgaaaccga cgcccagtac cagcgacaag gcaaagccgc
     2281 cgaacgcttc aataaacgta atcagggaat gatgaccgag gatcggcagc ttttttataa
     2341 atgcgctata caggtcgcgc ggcggcggta aaatcaacgc aggaaccttg atgacttcga
     2401 cgagaatctc ccacaaaatt aacaacacaa tgaagaagat caccggttcg gcgatgtttt
     2461 tccaggaagt tttcacagct gcactggcgc cgacgttaat ggaaaggctg gatggagtca
     2521 acaaattgcc tcggtcaaga cttcccttcg ataatacaat ctcggtgcac gagagtaatc
     2581 gtaaagtttg cgcctaccaa gcgatgtgtt agcaatgaat gaaggagtcg ctcaaacacg
     2641 cacgcgatgg cacaaataac tttcacacct ggtgagctct acgatgcttg gccggtgctc
     2701 tcgcagcccg aacgaatcga gggctttgaa ttcttgcgac gagacgatgc cgaatcgttt
     2761 tttctgcaat tgaatcttcg cgagcgaacc cgtttagtgc tcggtctgcc gcccaacgag
     2821 cggcgattat ggatgcggct gctcgctccc gacgacgccg ccgatctcat tcaggaagcg
     2881 ccggcgaacc agcgaacggc gctgcttgag ttactcgacg ccgccaccca gcgcgaagtc
     2941 aagggactcc tcgattacgc cgaggacgat gccggcggct tgatgaatac ccgttattcg
     3001 cggcttcgac ccgagatgac ggtcggcgag gcgatcagct acctgcgccg cgacgcgcag
     3061 gcgcggacgc gcacgacgta ctacgcctac gtcgtcgatc atgaagagcg cttgctcgga
     3121 gttatcacgt ttcgcgacct gattgtcgct ccggccgata aatcggttcg cgaaattatg
     3181 cgcacgcaaa tcatttcagc caacgaagaa atggaccagg aggcgctgag taaactattt
     3241 atgcggcatc acttattgat gatccccatc gtcgatgacg ccgggcatat caaagggatc
     3301 gtcagcgtcg atgacatcgt cgacgttgtc caggaagaag cgagcgaaga cattcaaaag
     3361 gtcggcggta tggaagcgtt ggacgctccc tatctgcaga tcggcttttg gcgaatggtg
     3421 aagaagagag ccggctggtt ggccattctc ttcgtgggcg ag
//
LOCUS       DATLCH010000191         2700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5739013, whole genome shotgun sequence.
ACCESSION   DATLCH010000191 DATLCH010000000
VERSION     DATLCH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5739013"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1176
                     /locus_tag="VMZ02_05640"
     CDS             76..1176
                     /locus_tag="VMZ02_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71448.1"
                     /translation="MDNSTTSMKLQRADNELGAKTTYQLFLEREGVPAIGGFHIEDIN
                     RVELASWPRVGGRGVYLNLEGSEGVNNCYVCEIAPGKSLEPQKHMFEALIYVVSGHGA
                     TTIWQQDGKKQTFEWGEGSLFSPPLNAWYQHFNGQGDRPVRLLAMTNAPTVLNLYHNI
                     DFIFNCDYRFADRYAGEDDFFNGQAKVPGEGFHDTNFIRDVRSYELPERNDRGAGGKM
                     LMIEMSNNVMSAHISQFPVGTYKKAHRHGAGAHVIVLKGEGFSVMWKEGDDIKRYNWR
                     AGSLMVPPERWFHQHFNVGGEPARYLALKPFSSRKFPGLRKQWGTSESVKLGGDQIEY
                     DDEDPRIRAMFDEELGKRGVMSRMGAVYKVAS"
     gene            complement(1291..1464)
                     /locus_tag="VMZ02_05645"
     CDS             complement(1291..1464)
                     /locus_tag="VMZ02_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71449.1"
                     /translation="MTRLQCLILLLLATLIVLFQSSLISYLSNQAVAFTLLGASLILL
                     AHQLRRYSNKSVS"
     gene            complement(1694..2110)
                     /locus_tag="VMZ02_05650"
     CDS             complement(1694..2110)
                     /locus_tag="VMZ02_05650"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HUR71450.1"
                     /translation="MIEILDQMKELIDPALANGCPCILATVSAEGEPDIGYKGSMMIF
                     DKQSLAYLERTKRVHLKNLKENPKVIVLFRDAKTKVAWRFHGTAELHESGPVWEQGMA
                     RTVKEELDKDPERKGIIVQIHVDKITNMGGQVLQSR"
     gene            complement(2478..>2700)
                     /locus_tag="VMZ02_05655"
     CDS             complement(2478..>2700)
                     /locus_tag="VMZ02_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71451.1"
                     /translation="TKFIAEEEREWNVCVVAFSPKQAIATEDAPEEARQRLPAPDSLG
                     NGFVLAAQAGMEMTGKTVEHKDGKLIVVD"
BASE COUNT          616 a          705 c          752 g          627 t
ORIGIN      
        1 tctcttgacc ccagcggttc ggggtcatac aaatgcgatg agttattgga atcgttacat
       61 cggaggcaat cgagcatgga taattctacg accagcatga agcttcagcg cgccgacaac
      121 gagttgggcg cgaagacgac ctatcaattg tttctcgaac gcgaaggcgt gccggccata
      181 ggcgggtttc atatcgagga catcaatcga gtcgaacttg cttcgtggcc ccgggttggc
      241 ggacggggcg tgtatctaaa tctcgaagga tccgaaggcg tcaacaactg ctacgtttgc
      301 gagatcgctc ctggcaaatc gctcgaaccg cagaagcata tgttcgaagc attgatctat
      361 gtcgtaagcg gtcatggggc gacaacgatt tggcagcagg acggcaagaa gcaaaccttc
      421 gaatggggcg aaggctcgct gttctcgccg ccgctcaacg cctggtacca gcactttaac
      481 ggtcagggcg acaggccagt gcgattgttg gcgatgacca acgcgccgac ggtgctcaac
      541 ctttatcaca acatcgattt catctttaac tgcgattatc gtttcgccga tcgttatgcc
      601 ggggaagacg atttcttcaa cggtcaggcg aaagtacccg gcgaaggctt tcacgatacc
      661 aactttattc gcgacgtgcg cagttacgaa ttgcccgagc gcaacgaccg cggcgccggc
      721 ggcaaaatgc tgatgatcga gatgtcgaac aacgtcatgt cggcgcacat ctcgcagttc
      781 ccggttggca cgtacaagaa agcgcaccgc catggcgccg gcgcccatgt gatcgttcta
      841 aaaggcgagg gcttttcggt gatgtggaaa gaaggcgacg atatcaaacg ctacaactgg
      901 cgagccggca gtttgatggt gccgccggag cgttggtttc accaacactt taacgtcggc
      961 ggcgagccgg cgcgctatct ggcattgaag ccgttcagca gccgcaagtt tcccggtttg
     1021 cgcaagcagt ggggcacttc ggaaagcgtc aaacttggcg gagatcagat cgagtacgac
     1081 gatgaagatc cgcgcattcg tgccatgttc gacgaggagc ttggcaaacg cggtgtcatg
     1141 agccggatgg gagcggttta taaggttgca agctgagtcg aggattcgct ctcatcacaa
     1201 agggcagcga gtcggcgcca gtcgacgaac gtgcaatagc tcccgcaaag acgcgtggtc
     1261 tgcggaggca ctgccgaaaa ccgggacctg ctaacttaca gatttgttgg agtagcggcg
     1321 caattggtgc gcaagaagaa ttagcgacgc gccgagcaat gtaaacgcca ccgcttgatt
     1381 gctcaagtat gaaatcaagc tggactggaa gagcacgatc aacgtcgcta gcaataataa
     1441 aatcaaacac tgtaagcgtg tcatatcggc ctcccttaga cgtggaatga aacaggcgcc
     1501 gatcctattc aaagtgtatc cgtgctgtca agcccctcca gcgttcgttt tcaatcggtt
     1561 ccagctttct cggttgatgt atttcccagc acacgaccgc tcgcaggcga aatgatttct
     1621 gccagttaga atcaattatt gccaattaga attaactgcc ggctttatgt cctgcggcta
     1681 agtgcgatcg ccgttagcgg ctctgtaaaa cttgtccgcc catgttggtg atcttatcga
     1741 catgaatctg cacgatgatg cctttgcgtt cgggatcttt gtccaattct tctttgaccg
     1801 tgcgcgccat gccttgctcc cagaccggtc ccgattcatg cagctcggcc gtgccgtgaa
     1861 aacgccacgc tactttggtc ttggcgtcac gaaacagcac gatgactttg ggattttctt
     1921 tcaaattttt cagatgaacg cgcttggtgc gttccaaata ggcgagcgac tgtttgtcga
     1981 aaatcatcat gctgcctttg tagccgatgt ccggttcgcc ttcggcggaa actgtggcga
     2041 gaatacacgg acaaccgttg gccaaagccg ggtcgatcaa ctctttcatc tggtcgagaa
     2101 tttcgatcat gagatacctc ctggagggct tttcttataa aactattgag cgatctgcaa
     2161 actgatccgc gagtatttcc cgcaaagacg taaagctctt acagttcgcg cgcggttcgg
     2221 gtaacacgaa gtattcatga gctgcaaaat attaaattca gcttccacat gcatcattct
     2281 ttcaatgatc atgccggccc aggtcgacac gagggcaggg acggatggat accggcttcc
     2341 gccggaatga caaaaccttc atcgcttgga gcgtacctaa catgtattct cgaaggaggt
     2401 acgaagggcg cacggagttt catagccatc caaaaaccgt cggtgtctcc gccttcttga
     2461 ctgtaatgga gtttccatca gtccaccacg atcagcttgc cgtctttgtg ctcgacggtc
     2521 ttgccggtca tctccatgcc ggcctgcgcg gcgaggacaa agccgttgcc gaggctatcg
     2581 ggagcgggta ggcgctggcg cgcttcttcc ggcgcgtctt cggtggcgat cgcttgtttg
     2641 gggctgaagg cgacgacgca gacgttccat tcgcgttctt cttcggcgat gaatttggtg
//
LOCUS       DATLCH010000192         7484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6974152, whole genome shotgun sequence.
ACCESSION   DATLCH010000192 DATLCH010000000
VERSION     DATLCH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6974152"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VMZ02_05660"
     CDS             complement(<1..396)
                     /locus_tag="VMZ02_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71452.1"
                     /translation="MAANETGGEVHNKKRAANLILLSVCLGQALVGLDQRAITVALPT
                     LAQIFNTPFTTIQWTILVYDLVLIGLIITMGRLGDLFGRRRFYTLGFIIFVIASAICG
                     LTQTTGQLIFFRAVQAIGGSMIAANGRAIV"
     gene            complement(408..1844)
                     /locus_tag="VMZ02_05665"
     CDS             complement(408..1844)
                     /locus_tag="VMZ02_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71453.1"
                     /translation="MTDSNVSEEQRHHSRRVLYQVSFGLALVAINHRAIQVALPTLTQ
                     AFHSDLSFIQWVLLVYDLCVIGLVLTLGRLGDLFGRKWIYVSGFLIFAAGSALCGAAQ
                     SSAQLILFRVFQGIGGAMIMANGRALVTVNSPASERGKALGITSMAFHVGYITGPTLG
                     GFIIDAVSWRWVFFLTIPLGLACAYSGWKILKERPASETRVQIDFAGAGYLLLTNVCF
                     LYALNRLPHRGIYDSLVLVFFAIAAATLYLFIRTEQKAPTPILSVSLFRNRLFTAANL
                     SLFFLTLTQSAVSVLIPFYLQNLMGFTPTQMGWILIGGSVVIIVLAPIAGRLSDRFGS
                     RLLCTVGAAIMMVGQYLIGSLTLRSTIFQMIMPQILIGLGWALFNSPSQSAIMSTVPR
                     DKVGAASGMTLTTGRIGGAMGIAISGAVMTYALSANGLTAAQIESPASWTAAPEVFLQ
                     SFAFTAHWITAFAILSVFFAAMRGARKD"
     gene            complement(1916..2080)
                     /locus_tag="VMZ02_05670"
     CDS             complement(1916..2080)
                     /locus_tag="VMZ02_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71454.1"
                     /translation="MSPAAKKIPIGLAKVRKELPPPGKILQNKKDVLRKRAKAELRRA
                     LKEQLSTSKS"
     gene            complement(2077..4704)
                     /gene="clpB"
                     /locus_tag="VMZ02_05675"
     CDS             complement(2077..4704)
                     /gene="clpB"
                     /locus_tag="VMZ02_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HUR71455.1"
                     /translation="MRLEKLTTKSQEALREAQALADKRNHQALDVEHLLFSLLGQKEG
                     VVLSLLQKLGVPLTQLTERLQKALDRLPQVTGASGQSYITPRLKKTIESAESEAEALK
                     DEYVSTEHLLLAMVQDSGETGKILKELGATRDKILGALVSIRGSQRITDRNPEEKYQS
                     LEKYGRDLTDLARKGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LALRIVNGDVPEGLKDKKLVALDLGALVAGAKFRGEFEDRLKAVLKEVTEASGQVVLF
                     IDELHTLVGAGAAEGAMDASNMLKPALARGELRCVGATTLDEYRKRVEKDPALERRFQ
                     QVYVGEPSVEDTIAILRGLKERYEVHHGVRITDPAIIGAATLSHRYISDRFLPDKAID
                     LIDEAASRLRIEIDSMPIEIDEVERKIMQLEIERQALKREQDKASIGRLAHLEQEIHN
                     LKETASGLKAHWQNEKAAIQQLRAIKEKIEATKVEEQKAQREGNLNKAAELRYGTLTQ
                     LEKESETVNRKLAELQSHQKMLKEEVDAEDIAEVVAKWTGIPVSKMLEGEVQKLLKME
                     ERLKLRVIGQENAIIAVSNAVRRARAGLQDQNRPIGSFIFLGPTGVGKTELCRALAEF
                     LFDDEQAMVRLDMSEYMEKHSVSRLIGAPPGYVGYDEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPDVFNALLQVLEDGRMTDGQGRTVDFKNTVIIMTSNLGSQFIQELAGKDRKQME
                     QLVNNALREAFRPEFLNRVDETIIFSSLGREEIKLIVDIQLKRLRQNLANRKMALEIS
                     EPAKALLAEKGYDPVYGARPLKRTIQRLIQDPLAVKILAAEFKEGDTVSVDVDDDELC
                     FSHGRPAASAEALEQRTLH"
     gene            complement(4938..5720)
                     /locus_tag="VMZ02_05680"
     CDS             complement(4938..5720)
                     /locus_tag="VMZ02_05680"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HUR71456.1"
                     /translation="MGNERISTGIGGLDALLHGGFNRGRTYLFAGEAGTGKTIACLQF
                     VAICLKQGDKAVYVTVDERPSEILDSAAALGWNLQPHIQEKQLIVLDAAPYFGTRGGG
                     EKGIDPQKIVADLGNYVRRLGATQLIIDPITPFILPPDSGSPPQDQARSLIQLLQTQI
                     GVTNLFTAQRCPDGAVSPEVRIEEHLAAGVFIFKLSESAAGCERTVTVKKMRGVAVAP
                     IKHRFTLQHDQGIVIDPPGSAPAESCQPTPMFEYFDPAKPQN"
     gene            complement(5742..6464)
                     /locus_tag="VMZ02_05685"
     CDS             complement(5742..6464)
                     /locus_tag="VMZ02_05685"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HUR71457.1"
                     /translation="MLQTERVSSGIKGLDPLIEGGFPKGRSILVTGEPGTGKTIFALQ
                     FLADGLTHGEKGIFVTADEGPLDVLEQAASLGWDFERHVENKELAILNAGTYLSSLPG
                     AGRERQIDLQKAVSDLAAFVNQIGAQRLVLDPAGPFVLLRDSAARIQDQTRLFIKLLR
                     SSMATTNILTSYAVPRTGERSMHGIEEYMVAGAIVLEMIWRNDGHLARSMIVEKMRCT
                     NVKPTQLEFDIVEHEGLVVQPG"
     gene            complement(6532..>7484)
                     /locus_tag="VMZ02_05690"
     CDS             complement(6532..>7484)
                     /locus_tag="VMZ02_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71458.1"
                     /translation="ETISARSENEAREIHQAKSALERELSGLRHELQQKSAAVAQQQA
                     AMDDLGARYRSHVEQLETNVREYQNTSEDRRREASEAQAQMAVLHKRLEELQSALQQT
                     ELAALNRDEQLRQEYEVRAAALSREAAEGSARHKEIARATSATEQALRGEIDRLIGEA
                     QERNLILQNRNDELVRVKGELDSLSERFTQLEAHAIQAENSASGDAERMRMEYQAQLA
                     LLQAELSQTEWAVEERQAIIAGIEQEHRHQIEILRQQLTEEQHPAAPVNGSLLISVAD
                     LTEPRRLEATFDAIEGDNGHPAAPGRRWQSSFGSKRRWRS"
BASE COUNT         1522 a         2105 c         2130 g         1727 t
ORIGIN      
        1 cacaatcgcg cggccgttgg ctgcgatcat cgaaccgccg atcgcctgga cggcgcgaaa
       61 aaaaatcaat tggccggtgg tttgggtcag gccgcaaatc gccgaagcga tcacaaaaat
      121 gataaatcca agcgtgtaaa aacgccgccg gccaaataga tcgccaagcc ggcccatggt
      181 gatgatcaaa ccgatcaaaa ccagatcgta aaccaggata gtccattgga tggttgtgaa
      241 gggtgtatta aaaatctgcg ccagtgtcgg caacgcgacc gtgatggcgc gctgatcgag
      301 accgaccaga gcctgaccga gacaaacgct cagcaaaatc agattcgccg cgcgtttttt
      361 gttgtggact tctccgccgg tctcgtttgc tgccatcgca gggttgccta atctttccgc
      421 gcgccacgca tggccgcgaa gaacaccgac aggatggcga aggcagtgat ccagtgggcg
      481 gtaaaggcga aggactgcag gaagacttcc ggtgctgccg tccaactggc cggcgactcg
      541 atctgcgcgg cggtgaggcc gttggcgctc aaggcgtagg tcatcaccgc gccggagatg
      601 gcgatcccca tggcgccgcc gattctaccc gtggtcaggg tcatgccgga ggcggcaccg
      661 actttgtcgc gcggaaccgt gctcatgatc gcgctttgac tgggtgaatt aaacagcgcc
      721 cagccgaggc cgatcaagat ttgcggcatg atcatctgga aaatcgtcga tcgcaaggtc
      781 agggagccaa tcaaatattg accgaccatc ataatcgcag cgccgaccgt gcacagtagg
      841 cgcgaaccga aacggtccga tagtcggccg gcgatcggcg ccaacacgat gatcaccacc
      901 gagccgccga tcaagatcca gcccatctgt gtcggcgtga aacccataag attctgcagg
      961 tagaacggaa tcaagacgct gaccgcggac tgggtcaaag tgagaaagaa aagacttaaa
     1021 ttagccgcgg tgaacagccg attgcgaaac agcgacacgc tgaggatggg cgtcggcgct
     1081 ttctgttcgg tgcgaatgaa tagataaagt gtcgccgcgg caatggcgaa aaagaccagg
     1141 accaggctat cgtaaatacc gcggtgcggc aggcgattga gcgcatagag aaaacagaca
     1201 ttggttagta gcagatagcc ggcgccggcg aaatcaattt gcacgcgggt ttcgctggcg
     1261 gggcgttctt tgagaatttt ccagccggag taagcgcagg caagtcccaa gggtatggtc
     1321 agaaaaaata cccagcgcca gctcacggca tcgatgatga agccgccgag cgtcgggcca
     1381 gtgatgtacc cgacgtgaaa cgccatggaa gttatgccca atgctttgcc gcgttcggac
     1441 gccggcgaat tgaccgtgac gagggctcgg ccgttggcca tgatcatcgc tccgccgatg
     1501 ccttgaaaca cgcggaataa tatcaattgg gctgacgatt gtgccgcgcc gcacaatgct
     1561 gaaccggctg cgaaaatcag gaagccagaa acgtagatcc acttacgacc gaagagatcg
     1621 ccaagccgcc cgagggtcaa caccagcccg atgacgcaaa gatcatagac cagcaaaacc
     1681 cattgaataa acgatagatc gctgtggaac gcctgggtga gagtgggtaa tgcgacttga
     1741 atagcgcgat ggttaatcgc caccaaggcc agcccgaacg atacttgata caacacccgc
     1801 cgggagtggt gccgttgctc ctcgctaaca tttgaatcgg tcatgaaaca aaaaataaag
     1861 agggggcagc cgagatcggc tgccccctct tctgatcatc tcttttcaga agaatttacg
     1921 acttagaggt gctcagttgc tccttgagcg cgcggcgtaa ctcggctttg gcgcgtttac
     1981 gcagaacgtc ttttttgttc tgcaagattt tccccggcgg cggcagttct ttgcgtactt
     2041 tcgccagacc gatgggaatc tttttagccg ctgggctcaa tgtagcgtcc tttgctccaa
     2101 ggcctcggcg cttgccgccg gacgaccatg agaaaagcag agttcgtcgt catccacgtc
     2161 cacgctaaca gtgtcgcctt ccttgaactc cgcggcaaga atcttaactg ccaatggatc
     2221 ctgaatcaat cgctggatgg tgcgcttgag cggtcgagcg ccgtagaccg ggtcatagcc
     2281 tttttcggcg agcaaggctt tggccggctc ggagatctcc aaggccatct tgcggttggc
     2341 caaattctgc cgcaggcgct tgagttgaat atcgacgatc aacttgatct cttcgcgacc
     2401 caggctgctg aaaataattg tctcgtcgac ccgattcaaa aattccggcc tgaaggcttc
     2461 gcgcaaggca ttgttgacga gctgttccat ttgcttacga tctttacccg cgagctcctg
     2521 gatgaactga ctgccgaggt tcgaagtcat gatgatcacg gtattcttga aatcgaccgt
     2581 gcgtccctgg ccatcggtca tgcggccgtc ttcgagcacc tgcaaaagcg cgttgaagac
     2641 gtcgggatgg gccttctcga tctcatcgaa tagcaccact gaataaggcc ggcggcgcac
     2701 ggcttcggtg agttgtcccc cctcatcgta gccgacatac cccggaggcg cgccgatgag
     2761 ccgcgacacg gaatgttttt ccatgtactc cgacatgtct aggcgcacca tcgcctgttc
     2821 gtcgtcgaaa agaaactcgg ccagcgctct gcatagttcg gttttaccca cgccggtcgg
     2881 tcccaaaaag ataaacgagc cgatgggccg gttctgatcc tgcaggccgg cgcgcgcacg
     2941 ccgcacagcg ttggaaacgg cgatgatggc attttcctga ccgatgacgc ggagctttaa
     3001 tcgctcttcc attttgagca gcttctggac ctcgccttca agcattttag acactggaat
     3061 gccggtccat ttggcaacta cttcggcgat gtcttcggcg tcgacttcct ccttgagcat
     3121 cttttgatgc gattgcaatt cggcaagttt gcgattgacc gtttcgctct ctttttccag
     3181 ttgagtcaag gtgccgtagc gcagttcggc ggctttgttc aaattgcctt cccgttgggc
     3241 tttctgttcc tcgactttgg tcgcctcgat cttttctttg atcgcgcgca actgttgaat
     3301 cgccgctttt tcattctgcc aatgggcttt gagcccgctc gcggtttctt tcagattgtg
     3361 aatttcctgt tcgaggtgcg ccaagcgccc gatagaggct ttgtcctgtt cgcgcttgag
     3421 cgcctggcgt tcgatttcca gctgcatgat tttgcgctcg acctcgtcga tctcaatcgg
     3481 catgctatcg atctcaattc tcaaacgtga tgccgcttcg tcgatcaaat cgatggcctt
     3541 gtccggcaaa aaccgatcgc tgatgtagcg atgcgatagt gtcgctgctc cgatgatcgc
     3601 tggatcggtg atgcgcacgc catgatgaac ttcatagcgt tccttcaagc cgcgcagaat
     3661 cgcgatggtg tcttccaccg acggttcgcc gacgtaaacc tgctgaaagc gccgttcgag
     3721 cgcgggatct ttctcgactc gcttgcgata ttcgtcgagc gtggtggcac cgacgcagcg
     3781 caattcgccg cgcgccaagg ccggcttcaa catgttcgag gcatccatgg cgccttcggc
     3841 ggcgccggcg ccgaccagcg tgtgcagctc gtcgatgaac agcacgacct gaccgctcgc
     3901 ttcggtgact tctttcaaaa cagccttgag tcggtcttca aactcgccgc gaaattttgc
     3961 cccggcaacc aaggcaccga ggtccaaggc gacgagtttt ttgtctttca aaccctccgg
     4021 tacatcgccg ttgacgatgc gcaaggcgag gccctcgacg atggcggttt taccgacacc
     4081 cggttcgccg atcagcaccg gattattttt ggtgcgacgc gacaaaactt gaatgacccg
     4141 gcgaatttca tcgtcccggc cgattaccgg atcgagcttg cccttgcgcg ctagatcagt
     4201 gaggtcgcgc ccatactttt ctaaagattg atatttttcc tctggattgc ggtcggtgat
     4261 gcgctgggag ccgcgaatgc tgactaacgc tcctaaaatt ttatcgcggg tcgcaccgag
     4321 ctctttaaga attttgccgg tctcgccgct gtcttgcacc atcgccagga gcaaatgctc
     4381 ggtcgagacg tattcgtcct tgagcgcctc ggcttccgac tcagcgcttt caatcgtttt
     4441 cttgagccgc ggtgtgatat agctttgacc gctcgcgcca gtgacctggg gtaaacggtc
     4501 aagcgctttt tgcaatcgct cggtcagctg ggtcagcggc acgccgagtt tctgcaagag
     4561 cgacaagacc accccttcct tctgtcctaa cagtgagaag agtaggtgtt cgacatcgag
     4621 cgcctggtga ttgcgcttat ccgccagcgc ttgcgcttcg cgtaaggctt cctgagattt
     4681 cgtggttagt ttttctaaac gcatgggtgt ctccgctgct gttgagatct cgtcgtcatt
     4741 gcgacaaatc ggcagccagc tgtcctggaa tgtaaactag gactttgtct tattcgccat
     4801 ttcgagagag attttaactt ctcccgcaga gagaacgcta cgacatactc tctgctttga
     4861 ggaagttaag catcgtcagg cgaggtgtca agaaaatcgc ctgaagggta acaaccgccg
     4921 ggtcggcgat gtgagtgcta attttgcggc ttggcgggat cgaagtattc gaacatcggc
     4981 gttggttggc atgactcagc cggcgccgat ccaggtgggt cgatgacaat accctggtcg
     5041 tgctgcagcg tgaaccggtg tttaatggga gcgacggcga caccgcgcat ttttttgacg
     5101 gtcacggtgc gctcacaacc ggctgcggat tcgctcagct tgaatatgaa cacaccagcg
     5161 gcgagatgtt cttcgatcct cacctcggga cttaccgcac cgtcggggca gcgctgcgcg
     5221 gtgaaaaggt tggtgacgcc tatctgggtc tggagcaatt ggatcagtga gcgggcttga
     5281 tcctggggtg gacttccaga gtcaggagga aggatgaatg gagtaatcgg atcgatgatc
     5341 agctgtgtcg cgcccagtcg tctcacatag ttgccaagat cggcgacgat cttttgcgga
     5401 tcgatgcctt tttcgccgcc gccgcgagtg ccgaaatagg gcgcggcatc gaggacgatc
     5461 aactgttttt cctggatatg tggttgcagg ttccagccca gagccgcggc agaatcgagg
     5521 atctccgaag gccgctcgtc gacggttaca tagaccgcct tatcgccctg cttcaagcaa
     5581 atcgcgacga actgcagaca ggctatggtt ttgcccgtac ccgcttcgcc ggcgaagaga
     5641 tacgtccgcc cccggttaaa ccccccgtgc agcaatgcat ccaaaccgcc gatgccggta
     5701 gagatccgtt cgttgcccat agacgatgct ctggcgagcg tttatcccgg ctggaccacc
     5761 agaccctcgt gctcgacgat gtcgaattcc agttgagtcg gtttgacgtt ggtgcagcgc
     5821 attttttcga cgatcatact gcgcgccaga tggccgtcgt tgcgccaaat catttccaga
     5881 acgatggcgc ctgcgaccat atactcctcg atgccgtgca tgctgcgctc gccggttcgg
     5941 ggcacggcgt atgaagtaag gatgttggtg gtcgccatcg aagaacgcag tagcttgata
     6001 aaaagacgag tttgatcctg gattctggcg gcgctgtcgc gtaacagcac gaagggtccg
     6061 gcgggatcga ggaccaaacg ctgggcgccg atctggttga cgaaggcggc caggtcgctc
     6121 acggcttttt gcagatcgat ctgtcgttcg cggccggcgc cgggaagcga gctcaaatac
     6181 gtcccggcat taagtattgc cagttctttg ttctcgacgt gacgttcgaa atcccagccg
     6241 agggaagcag cctgttcgag cacatctaag gggccttcgt ccgcagtcac gaaaatgcct
     6301 ttttcaccgt gggtcaaacc gtcggcgaga aactgcaggg cgaaaatggt cttgccggta
     6361 cccggttcgc cggtcaccag tattgaacga ccctttggaa atccgccttc gatcaatggg
     6421 tccaagccct tgatcccgct gcttacccgt tcggtttgta gcatcgttgt ccttcgctat
     6481 gctggagttt cttgcgcact tattgtgatt gctcgcggtt attcgcacag cctaagagcg
     6541 ccagcggcgt tttgatccga agctgctttg ccaacgtcgt cccggcgccg ctggatggcc
     6601 gttgtcaccc tcaatggcat cgaacgtggc ctcaagtcgc cgcggctcgg tcagatcagc
     6661 aacgctgatc agcaatgagc cattaaccgg tgcggctggg tgttgctcct cggtcagctg
     6721 ttggcgcaga atctctattt ggtggcgatg ctcctgctca attccagcaa taatcgcctg
     6781 gcgctcttcg acagcccact ccgtctggct aagttcggcc tgcagcagcg ccaattgcgc
     6841 ctggtattcc atgcgcatgc gctcggcatc gccactggca ctgttttccg cttggatcgc
     6901 atgagcctcg agctgcgtaa agcgctcact taagctgtcc agttcgcctt tgacccgcac
     6961 caactcatcg ttgcggtttt gtaaaatcaa attgcgttcc tgggcttcgc cgatgagccg
     7021 gtcgatttca ccgcgtaacg cttgctcggt cgcggaggtg gcccgggcaa tctccttgtg
     7081 gcgcgcggag ccctccgcgg cttcacggct gagcgcggct gcgcgtacct cgtattcctg
     7141 gcgcagctgt tcgtcacggt tgagagcagc caactcagtc tgttgcaatg cggattgcag
     7201 ctcttcgagt cgcttgtgta aaacagccat ttgcgcttgc gcctcggagg cttcccgacg
     7261 gcgatcttca ctggtgtttt gatactcgcg cacattcgtt tcgagttgtt cgacgtggct
     7321 gcgataacgc gcgcccaggt catccatagc cgcttgttgc tgtgccacag cggccgattt
     7381 ttgttgcagt tcgtggcgca ggccactgag ttcgcgctcg agagcgctct tggcctggtg
     7441 gatttcgcgc gcttcgtttt cactgcgtgc actgattgtt tcag
//
LOCUS       DATLCH010000193         5861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6225768, whole genome shotgun sequence.
ACCESSION   DATLCH010000193 DATLCH010000000
VERSION     DATLCH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6225768"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VMZ02_05695"
     CDS             complement(<1..956)
                     /locus_tag="VMZ02_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR71459.1"
                     /translation="MLLPFREHLDFGPTPYDLLQEKEGIPIVRGNVVNDVTTMELGKW
                     ERMGVLGCYLNLSNQQRTDAYVCEIPPSSQTLPQRHLFEEIVYVAKGRGATTVWQDEK
                     SKVTFEWGPGAIFAIPLNSTYIHFNGSGSEPIRLFVGTTCPAMINIFHNEDFIFRNPF
                     NFRDRFHEGDEFLRYNKHVTDRYWETNLVPDVNHFPLDDFPMKGKNVKHMRYTLADTT
                     YGCHVSEFPPGCRSTFHRHGPGAVIIITQGEGYVVLWRDGEERQRHEFKVGTVYSPND
                     LMWHGHFNTGKGNMRHFAMRGDSPKYSHDRFRNPAWTMIPMD"
     gene            1071..1469
                     /locus_tag="VMZ02_05700"
     CDS             1071..1469
                     /locus_tag="VMZ02_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HUR71460.1"
                     /translation="MRDSLRTGLTRTASYQTTIDMRARQLKRDVFSTPSMISLMERTC
                     TELTEPFLDDNEQTVGIHIDVYHLAATRIGQSVTINTEIIEVKGRKIRYAVDAYNDAG
                     VKIGAGTQQRAVVDTQRFAGGVDREADDAG"
     gene            1459..2541
                     /locus_tag="VMZ02_05705"
     CDS             1459..2541
                     /locus_tag="VMZ02_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71461.1"
                     /translation="MQAKITALAFAALLVLSTAIPSRLLAQSEEKLLDDINQLAEAER
                     QTRLINGARKEGIVNWYVAMNRVYANDLVNGFQSEYPFLKVNTLTGSGGSLLNRALTE
                     SRARTYQFDVFNTRSMTISTLKKAGAIMRYRSPQRANLRDGFADKEGYLNGIFATPLV
                     LLFNSKMVARKEAPSTVEDLLNPKWAGKMAIDDESYDWLAALMDYYGEAKGLELAGRL
                     GQQKLQVRRGPSLIAQLVAAGEFALQIDSHHQEVINKKKAGAPIDYNFPQPYVPAKSL
                     IPIYMSSHPPHPHAAALLADFLMSRKGQEIMHGHGRWVGHKNIASKGPDDVGERKVVI
                     PSADKWGDRYNELIGWYNKLLLRQSA"
     gene            2615..2788
                     /locus_tag="VMZ02_05710"
                     /pseudo
     CDS             2615..2788
                     /locus_tag="VMZ02_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            2810..3136
                     /locus_tag="VMZ02_05715"
     CDS             2810..3136
                     /locus_tag="VMZ02_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR71462.1"
                     /translation="MPKDLAERARVRIWVDYCNTRLQRAGGLVAHDYKVEKSKAEIVE
                     YLKFIDDHMRGRDYIAGDYSLADITFIPFFTRLERYQTNLGDDLPNVRNWVKRLLDRP
                     AVRETL"
     gene            complement(3149..3277)
                     /locus_tag="VMZ02_05720"
     CDS             complement(3149..3277)
                     /locus_tag="VMZ02_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71463.1"
                     /translation="MKASLPDVYCLVRHTGGCRYPGLFLSLSHYPAWMPVFTGMSE"
     gene            3276..4268
                     /locus_tag="VMZ02_05725"
     CDS             3276..4268
                     /locus_tag="VMZ02_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71464.1"
                     /translation="MIMRPLFTLLAFILLTISVQSLHAATALTKVLLTTGSVSEREAA
                     VYVAQEQGYFRKYGVDVQFIQARNGPVGMAALSSGESQFHWGSVSGANLGAIAEGADI
                     VFVAGFINRLTGMFMANSKIKSPAELRGKSVGVNSLSGGGWIFSMLTLDHWGLSPERD
                     KIQFRSLGDQTVMVQGLMAGIVDAALVGYAHGKNLQNKGYTVLADVEKLPIPYQGSGL
                     MSRRNFMRTQPAVAESVFRGLLDAVNVIRNPANKAEVLRAVAKGMRLAKVEDAEEGYK
                     SLPTLYDQKIYPSVDGIRNVIRLLGSTNEKIRKLKAEDLVEDAVLKKLEKEGRF"
     gene            complement(4401..5588)
                     /locus_tag="VMZ02_05730"
     CDS             complement(4401..5588)
                     /locus_tag="VMZ02_05730"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71465.1"
                     /translation="MSQVLPTAESDSGTAPRGSANVRHLCRMELEGGGQIVLDRNYAY
                     VGHQHRVQGTTILDIADPRKPKVVSTLMPNHPWSHSHKVRVVGDVMLVNSEFERGPGQ
                     RGDYPDGGFRIYDIKDRSNPKLITFVKTHGKGVHRFDCDENYAYISTEMDGFVGNILV
                     IYDIRNPTKPAEVSRWWMTGQHVAGGDAPHPKHAEHRLHHAMRCGSEMYAGCWMSGIS
                     IIDVSDIGTPRTLSHYEYDPPCPEPTHTFLKAAHPIAGKQIAVSTEEERTHRGADTGK
                     PHAPLRTWDVSDPTKPKLLSTTYLPESATPYPADKVRFGTHQLRERVDEDNLLYVTWF
                     AGGLRIIDINDPAKPKERGYFIPKPGDRVAAPLTNDVVKDHRGLLWVTDKERGLDVIE
                     FLN"
BASE COUNT         1329 a         1676 c         1626 g         1230 t
ORIGIN      
        1 tcatccattg ggatcatcgt ccaggccgga ttgcgaaatc gatcatggct gtatttggga
       61 ctgtcgccgc gcatggcgaa gtggcgcatg ttgcctttcc cggtattgaa gtggccatgc
      121 cacatcagat cgttgggcga gtagaccgtg ccgactttga attcatggcg ctggcgctcc
      181 tcgccatcgc gccacaacac gacgtaacct tcgccctgcg tgatgatgat caccgccccc
      241 ggaccatgac gatgaaaggt gctgcggcaa cccgggggaa actcagagac atggcagccg
      301 taggtcgtat cggcgagcgt gtagcgcata tgcttgacgt tcttaccctt catcggaaag
      361 tcatcgagtg ggaaatgatt aacatcaggc accaggttcg tctcccagta gcggtcggta
      421 acatgcttgt tgtagcgcag aaactcgtcg ccttcatgaa aacgatcgcg aaagttgaac
      481 ggattccgaa agataaaatc ttcattgtga aaaatattga tcatcgccgg gcaagtcgtg
      541 ccgacgaaca atcgaatcgg ctcgctgcca gaaccgttga aatggatgta ggtggagttc
      601 agcggaatgg cgaagatcgc cccgggtccc cattcgaagg taaccttgga cttctcatcc
      661 tgccacaccg tcgttgcgcc gcgccccttg gcgacgtaga cgatttcctc gaataaatgg
      721 cgctgcggca aggtctggct gctcggcggg atttcgcaga cataagcgtc ggtgcgctgc
      781 tggttggaaa ggttgaggta gcagcccaac actcccatgc gctcccactt gcccaactcc
      841 atcgtggtga cgtcgttaac gacattgcca cggacgatgg gaatcccctc tttttcctgc
      901 aaaagatcgt acggtgtcgg accaaaatcc aaatgctcgc gaaacggtaa cagcatcgtg
      961 taactcctcg gcaatttgtt catccatagc acgaacacgc gccacgatca cgtccttaat
     1021 ttcggttatt gactgttgcc tacccacacc ccctccccta tattcgcccc atgcgagata
     1081 gtttgcgcac cggcctaacc cgaactgcgt cttaccaaac tactatcgac atgcgcgcgc
     1141 gccagctcaa aagagatgtc ttctcgacac cgtcgatgat tagcctgatg gaacggacct
     1201 gcaccgagct gaccgagccg ttccttgacg acaacgaaca gacggtcggc attcacatcg
     1261 acgtttatca cttggcagcg acgcgcatcg gccagagcgt cacaatcaat actgagatca
     1321 tcgaggtgaa aggccgcaaa atccgttatg cggtggatgc ctacaatgat gctggtgtga
     1381 aaattggcgc gggcacccag cagcgcgccg tcgtcgacac tcagcgcttt gccggcggtg
     1441 ttgatcggga ggctgacgat gcaggctaag attacagcct tggcatttgc cgcgctgcta
     1501 gtgctgtcga cagctatacc gtcgcgactt ctggcgcaaa gcgaagaaaa actgctcgac
     1561 gacatcaatc agctggccga agccgagcgc cagacacgcc tgatcaacgg cgctaggaag
     1621 gaaggcattg tcaactggta cgtggccatg aatcgtgttt acgccaacga tctcgtcaat
     1681 ggcttccaaa gcgaataccc cttcctcaaa gtcaacacct tgaccggcag cggcggctct
     1741 ctgctcaatc gcgcgcttac cgaaagccgc gcgcgcacct atcagttcga tgttttcaat
     1801 actcgcagca tgaccatcag cacactcaaa aaagccggcg ccatcatgcg ctaccgctcg
     1861 ccccagcgcg ccaatctgcg cgacggcttt gccgacaagg aaggctattt gaacggcatc
     1921 ttcgccacgc cgttagtgct cctgttcaat agcaaaatgg tcgcacgcaa agaggcgccg
     1981 agcaccgtcg aagatttatt gaatcccaaa tgggccggca agatggccat cgacgacgag
     2041 tcctacgact ggctggcggc attaatggac tactacggcg aagccaaagg cttggagctg
     2101 gcaggccggc tgggccagca aaagctgcaa gtgcggcgcg gcccgtcgtt gatcgcgcaa
     2161 ctcgtggcgg ccggcgagtt tgccctgcag atcgacagtc atcatcagga agtcatcaac
     2221 aagaagaaag ccggagcgcc gatcgattat aattttcccc aaccctatgt cccggccaaa
     2281 tcgttgatcc caatctatat gtcgtctcat ccgccgcacc cccacgcggc ggcgttgctc
     2341 gccgactttc ttatgtcgcg caaaggccaa gagatcatgc atggtcatgg ccgttgggtc
     2401 ggccacaaga acatcgctag caaagggccg gacgatgtcg gcgagcgcaa ggtggtgatc
     2461 ccgtcggcgg ataaatgggg cgaccgttat aacgaactga tcggctggta caacaaattg
     2521 ctgttgcgcc aaagcgcata agcggcgtag attcagcgag taacctggag gctttatgaa
     2581 actattcacc taccggccct gaccttacgc gcaccggaca aggattgtcc tggcagaaaa
     2641 gaatcttccc tacgagacca tcgaatgcga tctcaagaac aagtccaagg aattgctcga
     2701 ccgcaatccc tacggcaaag tccccgtgct cgaagacgac agcgcggtga tctacgagtc
     2761 ggcggtcatc ggcgagtatc tcgacgagaa atatccggcg gtgcgcttaa tgccgaaaga
     2821 tctcgccgag cgcgcccgcg tacggatttg ggttgactac tgcaataccc gcttgcaacg
     2881 cgccggtggc ttggtcgccc atgattacaa agtcgaaaag tccaaggcgg agattgtcga
     2941 atacctaaaa ttcatcgacg accacatgcg cgggcgcgat tacatcgccg gcgattattc
     3001 gctcgcggat ataacgttca ttccgttttt cacccgcctt gagcgctacc agacaaacct
     3061 tggggatgat ctgccgaacg tcagaaattg ggtcaaacgg ttgttggacc gcccggcggt
     3121 gcgggagacg ctctagcccc cgaatcaact actccgacat gccggtgaaa accggcatcc
     3181 acgctggata gtgtgacaac gataaaaata agcctggata ccggcatccg ccggtatgac
     3241 ggacaagaca gtaaacatca gggagactcg ctttcatgat catgcgacct ttgttcaccc
     3301 ttctcgcgtt catacttctg acaatctcgg ttcaatccct ccacgccgcc accgcgctta
     3361 ccaaagttct tctcaccacc ggctcagtga gcgagcgcga agcggccgtc tacgtcgccc
     3421 aagaacaagg ctacttccgc aagtacggcg tcgatgtcca gttcattcag gcgcgtaatg
     3481 gtccagtcgg catggcggcg ctcagctccg gcgaatcgca gtttcactgg ggttcggtat
     3541 ccggcgcgaa cctcggcgcc atcgccgaag gcgccgacat cgtattcgtc gccggcttta
     3601 tcaatcgctt gaccggcatg ttcatggcca attcgaagat taaaagcccg gcggaattac
     3661 gcggcaagag cgtcggcgtc aacagcctca gcggcggcgg gtggattttt tctatgttga
     3721 cattggatca ttgggggctg tcgccggagc gcgacaaaat ccagttccgt tccctcggcg
     3781 atcaaaccgt catggtccag ggactcatgg ccggcatcgt cgacgccgcg ctggtcggct
     3841 acgcccatgg gaaaaacttg cagaacaaag gctacacggt gctggcagac gtcgaaaagc
     3901 tgccgatccc ctaccaaggt tcaggactta tgtcgcggcg caacttcatg cgcacgcagc
     3961 cggctgtggc ggaaagcgtc tttcgcggct tgctcgatgc agtcaatgtt attcgcaacc
     4021 cggccaataa ggcggaagtg ttaagggccg tcgccaaagg catgcgcttg gcaaaagtgg
     4081 aagacgccga ggaaggttat aaaagcttgc cgactttata tgaccagaaa atctacccca
     4141 gcgtcgacgg catccgcaat gttatccggc tgctcggctc gacgaatgaa aagattcgca
     4201 agttgaaagc tgaagatctg gttgaagacg cggtgctcaa gaagctcgaa aaggaaggcc
     4261 gcttctaagg ctctactcgc ataatcgccg ctgtctctgg ttcgtcattc cggcgcaggc
     4321 cggaatccag gttttcttcg agaaggtgaa aaccaagata gatgccggcc tgcgccggcg
     4381 tgacggattt actttccggg ttagttcaag aattcgatta catccagccc gcgttccttg
     4441 tcggtgaccc acagtagacc acggtgatcc ttcacgacat cgttggttag cggtgccgcc
     4501 acccgatccc ctggttttgg aataaagtag ccgcgctcct tgggcttggc cggatcgttg
     4561 atgtcgatga tgcgcaaccc gccggcgaac caggtgacgt aaagaagatt atcctcatcc
     4621 acgcgttcgc gtagctggtg cgtgccgaag cgcaccttgt cggcagggta aggcgtggcc
     4681 gattcgggta gataagtcgt cgagagcagc ttaggtttcg tcggatcgct gacatcccag
     4741 gtgcgcagcg gcgcatgggg cttgccggta tcggcgccgc ggtgagtgcg ttcttcctcc
     4801 gtcgataccg cgatctgctt gccggcgatc gggtgcgccg ccttgagaaa agtgtgggtc
     4861 ggctccgggc aaggcgggtc gtattcgtag tgcgatagcg tgcgcggtgt gccgatgtcg
     4921 ctgacgtcga tgatcgagat ccccgacatc cagcagccgg catacatttc gctgccgcaa
     4981 cgcatcgcgt gatgcagccg atgctcggcg tgctttggat gaggcgcatc accgccggcg
     5041 acatgctgtc cggtcatcca ccaacgggaa acttccgccg gtttcgtcgg attgcgaatg
     5101 tcatagatga ctaaaatatt gccgacaaac ccgtccattt ccgtcgagat gtaggcgtag
     5161 ttctcgtcac aatcaaatcg atgcacaccc ttgccatggg tcttcacaaa ggtgatgagc
     5221 ttgggatttg acctatcctt gatgtcgtag atgcggaatc cgccgtcggg gtagtctcca
     5281 cgttgccccg gaccgcgctc gaactcggaa ttgaccagca tgacatctcc gacgacacgc
     5341 accttgtgcg agtgcgacca cggatggttc ggcatcagcg tgctcacgac ctttggtttg
     5401 cgtgggtcgg caatgtcgag gatcgtcgtg ccctgaacac ggtgttgatg accgacatag
     5461 gcatagttgc gatcgaggac aatctgtcca ccgccctcta actccatacg gcagagatgc
     5521 cgaacgttcg ccgacccgcg tggcgctgtg ccgctatcgc tttcagctgt ggggagaact
     5581 tgggacatac gaaacatcca agtaaattta cctgcgcctg taaccggttc ggaactgaca
     5641 gaatcatcgt cggcgtgagg cgatgaaacg gaactgctgc gacgtgaaga tcgattcttg
     5701 ctttttcgtc attccgacgc acgccggaat ccaggtttct tggccttact acaagcggag
     5761 gtcgctaaca atgtcgacta acgggagaaa tctgccgccc cttagaaaat caggtgatcg
     5821 ctcgaaccta acaacaatct cagaaaaatc tgcgacctgt c
//
LOCUS       DATLCH010000194         9231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1278840, whole genome shotgun sequence.
ACCESSION   DATLCH010000194 DATLCH010000000
VERSION     DATLCH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278840"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /gene="hflX"
                     /locus_tag="VMZ02_05735"
     CDS             <1..1042
                     /gene="hflX"
                     /locus_tag="VMZ02_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HUR71466.1"
                     /translation="LVILDEDLSPAQQRNLENALNTRVVDRSQLILDIFAKRARSNEG
                     KLQVELAQLEYLLPRLTRQWTHLSRLGGGIGTRGPGETQLEVDRRRIRERIGHLKRRL
                     TTVERTRSLQRRERDEVPFATVALVGYTNAGKSTLMNLLTRAGVFVEDKLFATLDPTT
                     RALRLPNGDKVMIVDTVGFINKIPHSLIEAFKSTLEEVNRADLLLHLVDLANPLYEEQ
                     IQIIDKVLGEIGAGEIPTILVPNKIDLAGAVPLAGLKTNGVAQVCPISALTGTGVDEL
                     LTGVGRILDQSKERIEVQFSPAQSWLVALLRERGRVVEEHHDGETIKVAALVTPKLAG
                     QIRKALRSSRPR"
     gene            1048..1632
                     /locus_tag="VMZ02_05740"
     CDS             1048..1632
                     /locus_tag="VMZ02_05740"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HUR71467.1"
                     /translation="MKLSRRMLNLPNALTLIRILTVPIFLECLAYRLYWEALVVFAFG
                     GITDFFDGLTARWLNQQTALGAYLDPVADKLLVISSFIMLGLIDGIPMWLTVVVVVRD
                     ILIVVGYGAIYLLVEEKMAVRPSRIGKWSTSLQLLTLAVALVELHDATLVHPYVLKVL
                     IALSASATVASGLQYLYRGLIWLQNRAPSITPPG"
     gene            1791..1865
                     /locus_tag="VMZ02_05745"
     tRNA            1791..1865
                     /locus_tag="VMZ02_05745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1823..1825,aa:Val,seq:gac)
     gene            1883..3793
                     /gene="thrS"
                     /locus_tag="VMZ02_05750"
     CDS             1883..3793
                     /gene="thrS"
                     /locus_tag="VMZ02_05750"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HUR71468.1"
                     /translation="MDSLHVTFVDGNAVDARPGTRVVELAAAAGAKNLVAAKIDGQLV
                     DLNRVLDQDCVVEWVTPESPEGLDVLRHSTAHLMAQAVQSLFPGTQVTIGPTIADGFY
                     YDFKRDQAFSPEDLELIEKRMQELVKANLKVVREELPRQKAIELFRSMGEAYKVEIIE
                     GIAAEEVSLYRQGDWVDLCRGPHVPSTGSIKAFKLTSIAGAYWRGDSRNEQLQRIYGT
                     AWATKKDLDAYLQRIEEAKRRDHRRLGPALDLFSIHPIAPGSPFFHPKGTIIYNILIQ
                     YIRDLYKRFGYSEVVTPLIYKTELWKTSGHYEAFHDDMFLMNVHDGEYGVKPMNCPGH
                     CYLFSSRKYSYRDLPIRFADFSRLHRFEPSGVLSGLTRVRSFAQDDAHIYCTPEQLDG
                     ELEQFVTMTREVYRVFGFERIEVTLQTRPEKFLGRIELWDAAEAALRRCLERSGFSVK
                     ILPGEGAFYGPKIGFDFRDVLERSWTLATVQIDCAMPERFGLKYVTADGTEASPVMLH
                     RAVLGSIERFIAILLEHCAGAFPLWLAPVQLKVLTVTDDHKEYAVRVVDLLKGDGWRV
                     EFDARNEKLGYKIREAQLAKIPYAVIIGDKEVAAESVAPRRRGGENVTPLPVSEFMRN
                     LHAEVAQEIGEA"
     gene            3855..4337
                     /gene="infC"
                     /locus_tag="VMZ02_05755"
     CDS             3855..4337
                     /gene="infC"
                     /locus_tag="VMZ02_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HUR71469.1"
                     /translation="MNHQIRAREVRIIGSKGEQLGVMPLHEALKQVEALELDLVEVAP
                     DAQPPVCRMMDYGKFRYEQKKKQHGPKKTVVEIKEVKMGSRTDGHDVEHKVKNIRKFI
                     EEGQRVKVSVFFRGREITHPELGRQMLSGVVELVQDVAKVDIQPRLEGRSMAMLLTPK
                     "
     gene            4370..4567
                     /gene="rpmI"
                     /locus_tag="VMZ02_05760"
     CDS             4370..4567
                     /gene="rpmI"
                     /locus_tag="VMZ02_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HUR71470.1"
                     /translation="MPKIKTNRAAAKRFRVTGSGKIKRNKAYKRHILSSKGKKRKRNL
                     RKGTTVAAVEAKNIRQMIPYK"
     gene            4658..5017
                     /gene="rplT"
                     /locus_tag="VMZ02_05765"
     CDS             4658..5017
                     /gene="rplT"
                     /locus_tag="VMZ02_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HUR71471.1"
                     /translation="MPRAKGGSKSRQRHKKIMKLAKGYVGGRGRLYRSARETVERGLV
                     FAYRDRRVKKRTFRGLWIIRIGAAAKLNGMSYNQVISGLKKANVEIDRKILAEIAIAD
                     MPGFGRLAEIAKAHLSQ"
     gene            5038..6078
                     /gene="pheS"
                     /locus_tag="VMZ02_05770"
     CDS             5038..6078
                     /gene="pheS"
                     /locus_tag="VMZ02_05770"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HUR71472.1"
                     /translation="MTDSLEALRGEVLERIQQANSGKELEQLRVETLGRSGSITLLLR
                     GLKDLPAEERPRMGERLNQLRRALEEELDARLDSVNRQAKSRALAEEQIDITLPGTRF
                     KRGHIHPLTLVIDEIIDIFYGMGFEIARGPDIEDDYHNFEALNIPKDHPARDMQDTFF
                     VPGGNLLRTHTSPVQIRTMESRKPPLQIIVPGAVYRHDDDATHSPMFHQVEGFMVDKN
                     ISFSDLKGVLTHVLREIFDRDAGVRFRPSFFPFTEPSAEIDIQCVICGGGGTLRSGQV
                     CRVCKASGWLEILGAGMIDPAVFNAVGYDPEEFTGFAFGMGVERIAMLKYGVDDIRLF
                     FQNDLRFLRQFN"
     gene            6163..8241
                     /gene="pheT"
                     /locus_tag="VMZ02_05775"
     CDS             6163..8241
                     /gene="pheT"
                     /locus_tag="VMZ02_05775"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HUR71473.1"
                     /translation="MKFTLNWLKEFVDFDGSADELSKLLTMAGLEVESFTSLREPDNQ
                     REDWLFEISVTPNRGDCLGVLGIAREVSALTGGADVRFPPVHASKNDSTTRKRIAITI
                     EEGAPCARYSARIVDGIEVAPSPAWLRFRLEACGIRAINRIVDATNYVMLETGQPLHA
                     FDLARLTAGQIVVRNARKNLKFVTLDGIERELAPDDLVICDGDTPVAIAGVMGGSNSE
                     VSDATQSILLESANFAPAAIRRTAKRLALHSEASHRFERGVDPEGTIRALDRTVQVLL
                     ENGGGHPSLGVADCYPNIIAPVKLRLRDQRIEKLLGIGIEPKRAETLLRSLGMKTSRH
                     PKYLHVIVPTSRPDLTREVDLIEEVARLHGYDKIPTTLPQLRPTGGTRDAKLIAERKL
                     RHFFAGEGFVETINLPFTSDSLNNLCSGIWEGRQSAVPLLNPLAKENSAMRQSLLPGL
                     IESLRVNLAHKTTGFYGYQLGKVFRCNPEGGYEERLCVGAVMHGRPALGGLQRKEGPV
                     NYLECKGTVEGLFDLFGVGDSIGWNRTESAILHPGRAAQIVFKEHRIGLIGELHPDIS
                     EQLEVPQVYVFELDFEKLLEYAPRQMAVRALPKFPSIERDFAIVVDRDFVSERIVNWI
                     KNLGESLIEYVQVFDQYIGAPIPDGKKSLAYNISYRAEDRTLTDSEVNPLHQSLIDRI
                     GKEFAVERRS"
     gene            8277..8600
                     /locus_tag="VMZ02_05780"
     CDS             8277..8600
                     /locus_tag="VMZ02_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HUR71474.1"
                     /translation="MTKAEIITRIYEKVGFSKKDATDVVEATFDIIKGCLEKGDKVKI
                     SGFGNFVVNTKRPRKGRNPQTGDEIIIDGRKVLTFKASQIMKKSLNTMGSSSSVSAPD
                     APASE"
     gene            8611..8964
                     /locus_tag="VMZ02_05785"
     CDS             8611..8964
                     /locus_tag="VMZ02_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HUR71475.1"
                     /translation="MTPTLPEKIYFKIGEVSQIVGVEPYVLRYWETEFDLLKPSKAPS
                     KHRLYKKRDVELLLDIKRLLYTEGFTIEGARKKLREVKKEEKDQLKLPLAEQKYKTAL
                     IKVKKDLEALRKLVS"
BASE COUNT         2139 a         2388 c         2614 g         2090 t
ORIGIN      
        1 cttggtgatc ctcgacgagg atctcagccc ggcgcaacag cgcaatctgg aaaacgcact
       61 aaacacccgt gtggtcgatc gcagtcagtt gatcctcgat attttcgcta agcgggcgcg
      121 cagcaacgaa ggcaagctgc aggtagagtt ggcgcagttg gaatacttgc tgcctcgatt
      181 gacgcgccag tggacccact tgtcacgctt gggcggcggc attggtaccc gcgggcccgg
      241 tgagacgcaa ttggaggtcg accgccggcg cattcgcgag cgcattggcc atttgaagcg
      301 ccgcctgacc actgtcgagc gcacccgtag cctgcaacgc cgcgagcgcg atgaagtgcc
      361 cttcgctacg gttgcactgg tcggctacac aaacgccggc aagtcgacct tgatgaatct
      421 gcttacccgc gctggagtgt ttgtcgagga taagctcttc gcgaccctgg atccgacgac
      481 acgagcgctg cgattgccca acggcgacaa ggtcatgatt gtcgacacgg tcggtttcat
      541 caataagatt ccccattcat tgatcgaggc gttcaagagc acgttagaag aggtgaaccg
      601 tgccgacctg cttttgcacc tggtcgacct ggccaatcct ctctacgagg agcaaataca
      661 aataatcgac aaagttttag gcgagatcgg cgccggtgag attccgacaa ttctagtgcc
      721 gaacaaaatt gatttggccg gcgcggttcc cttggcgggt ctgaaaacaa acggtgtcgc
      781 gcaggtctgt ccgatctcgg cgttgaccgg caccggtgtt gacgaactgc tcaccggcgt
      841 cggtcgcatc ctggatcagt ccaaagagcg catagaagtt cagttttcgc cggcgcagag
      901 ttggttggta gcgctcctgc gtgagcgcgg ccgagtagtt gaagaacacc acgatggcga
      961 aaccataaaa gtcgccgctt tggtcacgcc aaagctcgcc gggcagatcc gcaaagcctt
     1021 gcgttcgagc cggccgcgct aacggcagtg aagcttagcc gccgcatgct caacctgccc
     1081 aacgctctca cgcttatccg tattctcacc gtgccgattt ttctcgagtg tctcgcttat
     1141 cgcctttact gggaggcgtt ggtggttttt gcgtttggcg gtattaccga ctttttcgac
     1201 ggattaactg cgcgctggtt aaatcagcag acggcgttgg gcgcttacct cgacccagtg
     1261 gcggataagc tactggtgat cagttcgttc ataatgcttg gactcatcga tggcataccg
     1321 atgtggctta cggtggtcgt ggtggtgcgc gatattttaa tcgtcgtagg ctacggcgcg
     1381 atttatctgc tggttgaaga gaaaatggcg gttagaccta gtagaatcgg taagtggagc
     1441 acaagtttac agttgttaac gttggcggtt gctttggtgg aattgcacga tgcgactctg
     1501 gtgcaccctt acgtcttgaa ggtcttaatc gctctgagcg cctcggcgac ggtcgcttca
     1561 gggctccaat atctctatcg cgggctcatt tggttgcaga atcgggcgcc atcgatcact
     1621 ccgccgggtt gacataatct ctgacaattg gtaataaaat cgatctcttg cagcattcct
     1681 gggctcgcac ctcagcgctt gggttggcca gattttcgcg acgattaata gaatgcctct
     1741 tcttttcgac aatttatcgg ccgtcaatgg cccaattctt cacccctttc aggcacgtag
     1801 ctcagtggga gagcgcttcc ttgacacgga agaggtcggc agttcaatcc tgcccgtgcc
     1861 taccatcttt taatttgcgg tcatggatag tcttcacgtc acctttgttg acggcaacgc
     1921 cgtcgacgct cgccctggta cacgggtagt cgaactggct gctgccgccg gggccaagaa
     1981 cctggtcgcc gccaaaatcg acggtcaact tgtcgaccta aatcgagttc tcgatcaaga
     2041 ttgcgtcgtc gagtgggtca caccggagag ccccgagggc cttgatgttt tacgccactc
     2101 cactgcgcat ttaatggcgc aggcggtgca gagccttttc cccggcaccc aggtcaccat
     2161 cggcccaact atcgccgatg gtttctatta cgacttcaaa cgcgatcaag cgttctcgcc
     2221 cgaagatctt gaattgatcg aaaagcgaat gcaggaattg gtcaaagcca acttgaaagt
     2281 cgtgcgcgag gaactgccgc ggcagaaagc catcgagcta tttcgcagca tgggcgaggc
     2341 ctacaaggtc gaaatcatcg agggtatcgc cgccgaagag gtctcgctgt atcggcaggg
     2401 ggattgggtt gacctgtgtc gtggccctca tgtgccatcc accggttcga taaaagcctt
     2461 caagttgacc agcattgccg gcgcttattg gcgcggtgac tcgcgcaacg aacagttgca
     2521 acggatttac ggcactgcgt gggcaactaa gaaagatctc gacgcttact tgcagcgcat
     2581 cgaagaggcc aagcggcgcg atcatcgacg ccttgggccg gcattggatc tattctcgat
     2641 tcatccgatc gccccgggat cgcctttttt ccatcccaag gggacgatca tttacaatat
     2701 tctgattcag tacatacgcg atctgtacaa gcgcttcggc tactcggaag tggtcacgcc
     2761 gctgatctat aagactgagc tgtggaaaac ctctgggcac tacgaagctt ttcatgatga
     2821 tatgtttttg atgaacgttc acgacgggga gtacggcgtc aagccaatga attgtccggg
     2881 ccattgttat ctatttagtt cgcgcaagta ctcctatcgc gatctgccga tacggttcgc
     2941 cgacttcagc cgcttacacc gatttgagcc ttccggcgtg ctgtcgggct tgacgcgggt
     3001 gcgctccttc gcgcaagacg acgcgcatat ctactgtacg cccgagcaat tggacggcga
     3061 gcttgagcag ttcgtgacca tgactcggga ggtttatagg gtattcggct tcgagcgcat
     3121 cgaagtaact ttgcaaaccc gcccggaaaa gtttcttggc cgtatcgagt tgtgggatgc
     3181 cgcggaggcg gctctgcggc ggtgtctcga gcgctcgggt ttttccgtga agatcttacc
     3241 tggggagggg gctttttatg gtcccaaaat cggattcgat ttccgcgatg tcctggagcg
     3301 ctcctggacg ttggcgaccg tgcagattga ttgcgctatg cccgagcgct tcggtctgaa
     3361 gtacgtgacc gccgatggca ccgaggcatc acccgtgatg ttgcatcggg cggttttggg
     3421 ctcaatcgag cgttttattg cgatcttgct ggagcattgc gccggggcat ttccgctgtg
     3481 gctcgccccc gttcaattga aagttctgac cgttaccgac gatcataaag aatacgccgt
     3541 tagagtcgtt gatctgttga aaggtgacgg atggcgcgtt gaattcgacg ctcgcaacga
     3601 aaagctcggc tataaaattc gcgaggctca actcgccaaa atcccgtatg ccgtcattat
     3661 tggcgataag gaagtcgccg cggaatcagt ggcgcctcgg cggcgcggcg gcgaaaacgt
     3721 cacgccactt ccggtgtcag agttcatgag gaatctccac gccgaggttg cacaagaaat
     3781 cggagaggca tagaaataca agatcatgct gcacgcagta actacggagg tgtcttatcg
     3841 ctagagaagc gcgaatcaat catcaaattc gtgcccgtga agtccgcatc atcggctcaa
     3901 aaggcgaaca gcttggagtg atgccattgc acgaggccct gaagcaggtg gaagccttgg
     3961 agctggatct ggtcgaagtc gcgccggacg cacaaccgcc ggtttgccga atgatggact
     4021 acggcaaatt tcgttacgag caaaagaaga aacagcatgg acccaaaaag accgtggtcg
     4081 agatcaagga agttaaaatg ggctcgcgca cggacggtca cgatgtcgag cacaaggtca
     4141 aaaatattcg caagtttatc gaagagggcc agcgtgtgaa ggtgtcggtg tttttccgcg
     4201 gccgcgagat cacccatccc gagctgggac ggcagatgct cagcggtgtc gttgagttgg
     4261 tgcaggatgt cgcaaaggtc gacatccagc cgcgtctcga gggccgcagc atggctatgc
     4321 tactgacacc caagtgaact taaattgaag tgaacgagag ggagtgaaag tgccaaaaat
     4381 taaaaccaac cgagcagcag cgaagcgttt tcgtgtgaca gggagcggca agatcaaacg
     4441 caacaaggct tacaagcgcc atatcttgtc gtccaaaggt aaaaagcgca aaagaaattt
     4501 gcgcaagggc accaccgtgg cggcggtcga agccaagaac atccgtcaga tgattccgta
     4561 taaataaccg ccgggtggct gcgaattagc agggcgcgtg atgcacacga ccctgtaccc
     4621 gggcggttga aaatctcgat tgagaggagt ttgtaaagtg cctagagcta aaggtggaag
     4681 caagtcgcga cagcgacata agaaaataat gaagcttgcc aagggatacg tcggcggtcg
     4741 cggccgtctg tatcgctcgg cgcgcgagac ggtcgaacga ggattggtgt ttgcgtatcg
     4801 tgaccggcgt gtaaaaaaac gtactttccg cggcctgtgg atcattcgca tcggtgctgc
     4861 ggcaaagcta aacggaatgt cttacaatca agtgattagc ggcctgaaga aggccaatgt
     4921 cgagattgac cgcaaaattc ttgcggaaat tgcgattgcc gacatgccgg gcttcggccg
     4981 cttggcggaa atcgctaaag ctcacctgag ccagtaacca ctcttcgttt cagtgccatg
     5041 accgattccc tggaagctct ccgaggagag gttctggagc gcatccagca agctaactcc
     5101 ggcaaggagc ttgaacagtt gcgcgtcgag actttgggac gcagcggttc gatcactttg
     5161 cttttgcgcg gcctaaaaga cctgccggca gaagaacggc cgcgcatggg cgagcggcta
     5221 aaccagttac gtcgcgctct cgaggaagag cttgatgcgc ggctggactc ggtcaatcgg
     5281 caagcgaaat cacgagcgct cgccgaagag caaatcgata tcactctgcc agggacgcgc
     5341 tttaagcgcg gccacattca tcctctcacg ctggtgatcg acgagatcat cgacatcttt
     5401 tacggcatgg gcttcgagat cgcccgcgga ccggatatcg aagacgatta tcataacttc
     5461 gaagcgctca atattcccaa ggaccatccg gcgcgtgaca tgcaggatac tttttttgtc
     5521 ccgggcggca atttgctgcg aacccatact tcgccggtac agattcgcac catggagagt
     5581 cgtaagccgc ccctgcagat aattgttccc ggcgccgtct accggcatga cgacgatgcg
     5641 acccattcgc cgatgttcca tcaagtcgaa gggttcatgg tcgacaaaaa tatttcattt
     5701 tcggatctaa agggcgtgtt gactcatgtg ttgcgggaaa tcttcgatcg tgacgccggt
     5761 gtgcgttttc ggccgagttt ttttccgttc accgagccta gtgccgagat cgatattcaa
     5821 tgcgtgatct gcggcggtgg cggcactttg cgcagtggcc aagtatgccg ggtttgcaag
     5881 gcgagtggct ggttggaaat tcttggcgcc ggcatgatcg accctgcggt gttcaacgcg
     5941 gtcggctacg acccggagga gtttaccgga tttgccttcg gcatgggtgt cgaacgaatc
     6001 gccatgctga agtatggcgt cgacgatatc cgtttgtttt ttcaaaatga tctcaggttc
     6061 ttgcggcagt ttaattaacg gaaccatgga gtaggtaagt gcgggagtgt agaattcaaa
     6121 actcattacc tcactgagcg agcgtttcga cactcctagt ctatgaaatt taccctcaac
     6181 tggcttaaag aattcgtcga tttcgacggc agcgccgatg agctttccaa actactcacg
     6241 atggcagggt tggaagtgga gtcgtttacg tcgctgcgcg agcccgataa ccagcgcgaa
     6301 gattggctgt ttgaaatctc cgtgacgccc aaccgcggtg actgtctcgg cgtgctggga
     6361 attgcgcggg aagtttccgc cttgaccggt ggtgctgacg tgcggttccc gcctgttcat
     6421 gccagcaaaa atgattctac cacccgtaag cgcattgcga tcacgatcga ggagggcgcc
     6481 ccgtgcgccc gttactcggc gcgcatcgtt gacggtatcg aggtcgcgcc gtcgcccgct
     6541 tggctgcgct ttcggctaga agcctgcggg attcgcgcga tcaatcgcat agtcgatgcg
     6601 acaaattacg tcatgcttga aaccggtcag ccgctgcacg cttttgattt ggcgcggctc
     6661 accgccgggc aaatcgtcgt ccgcaacgct cgtaagaatc ttaaatttgt aactctggac
     6721 ggtattgagc gcgagctggc ccccgacgac ttggtgattt gcgacggtga cactcccgtg
     6781 gcgatcgccg gagtcatggg cggtagtaat tctgaagtca gcgacgccac tcagtccata
     6841 cttctcgaga gcgctaattt tgcgccggct gcgattcggc gaaccgccaa gcggttggcc
     6901 ttgcatagcg aagcgtcgca tcgttttgag cgcggggtcg atcccgaagg gacgatccga
     6961 gcgctcgacc ggacagtgca agttttactt gagaacggcg gcggtcatcc ttcgctcgga
     7021 gtggcggatt gctacccgaa cattatcgcg cctgtgaagc tgcgcctgcg cgaccagcgc
     7081 attgaaaaac ttttaggcat tggtatcgaa ccgaaacgag ccgagactct actgcgttct
     7141 ctggggatga agacgagccg gcatcccaaa tatttacacg tcatcgtgcc aaccagccgt
     7201 ccggacctta ctcgagaagt tgacctgatc gaagaagtgg cccggttgca cggctacgac
     7261 aagattccga cgactttgcc gcagctgcgg cccaccggcg gtacgaggga tgcaaagctc
     7321 atcgcagagc gcaagctgcg gcattttttt gccggcgaag gctttgtcga gactatcaac
     7381 ttgccattca cgtcggactc tctcaacaat ttgtgttcgg gaatttggga gggtcgccaa
     7441 agcgctgtgc cattgctcaa tccattggct aaggaaaata gtgcgatgcg ccaaagcctg
     7501 ttacccggat tgatcgagag cttacgagtg aatttggcgc acaagacgac gggtttttac
     7561 ggctatcaac taggaaaagt ttttcgctgc aacccagaag gcggctatga agaacgtttg
     7621 tgcgttggtg cggttatgca tgggaggccg gcacttggcg gtctgcagcg caaagaaggc
     7681 ccggtaaatt atctcgaatg caaaggaacg gtcgaaggac tattcgatct attcggtgtc
     7741 ggcgattcga taggttggaa tcgcaccgaa tcggctattc tgcatcccgg acgggcagcg
     7801 caaattgttt tcaaagagca tcgaattggg ctaatcggtg agcttcatcc ggacatctca
     7861 gagcagctag aagtcccgca agtttatgta tttgaacttg acttcgagaa actgcttgag
     7921 tatgctccgc ggcaaatggc agttcgagcg ctgcctaaat tcccatccat agagcgagac
     7981 tttgccattg tcgtagatcg tgatttcgtc tcggaacgaa ttgtcaattg gatcaaaaac
     8041 cttggcgagt ctttaattga gtacgtccaa gtgttcgatc aatatatcgg cgcaccaatt
     8101 ccagacggca aaaaaagctt agcttataat atctcataca gagcagagga ccgaacactt
     8161 acggacagtg aggtcaatcc attacatcaa agtttgatcg accgaatcgg caaagaattc
     8221 gctgtcgagc ggcgcagcta gccggaccgc gctgcagtac agcgaggagg tgagacatga
     8281 ccaaagcgga aatcattacg cgcatttatg aaaaggtggg cttttcaaaa aaggacgcca
     8341 ccgatgtcgt cgaggcgaca ttcgatatta tcaagggctg tttggagaaa ggcgacaaag
     8401 taaaaatttc cggcttcggt aactttgtcg tcaataccaa acgtccgcgc aagggtagaa
     8461 atccccaaac cggcgatgag atcatcatcg acggccgcaa agtcttgacc tttaaggcca
     8521 gtcaaatcat gaaaaagagc ctcaatacga tgggatcttc atcgtcggtt tcggcgcccg
     8581 acgcgcctgc gtcggagtag gttcagacga atgacgccca cactgccgga gaaaatctat
     8641 tttaaaatcg gtgaggtcag tcagattgtc ggagtagagc cgtacgtact gcgttattgg
     8701 gaaaccgagt tcgatcttct caaaccctcc aaggcgccct cgaaacatcg actctacaag
     8761 aaacgcgatg tcgaactctt actcgacatc aagcggctgc tttatacgga aggttttacc
     8821 atcgaaggcg cgcgcaaaaa gttaagagaa gtaaagaaag aagaaaaaga ccagctgaaa
     8881 ctgccgctcg ccgagcaaaa atacaaaacc gcgttgatca aagtcaaaaa agatctcgag
     8941 gcgctgcgta agctggtgtc atagaaaaca gagttcttgc ccgcaaaaga caattttaat
     9001 tcgctaccgc ttaaccaatc ttcgagttat ttgccttcta tcactcaatg ttctaggtgt
     9061 tccggaaacc ctcgtccccg ttgaaagcta cacgtaggcg ttgtgctaat agtggagcca
     9121 gagtgcgtgc gttgttgccg cttatcatgt caccggaatc cttacctggc aaagcatgaa
     9181 aagagatcgg accgcgcgac agtgtggccc accgagaact tttggcgaaa c
//
LOCUS       DATLCH010000195         8779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7717088, whole genome shotgun sequence.
ACCESSION   DATLCH010000195 DATLCH010000000
VERSION     DATLCH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8779
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7717088"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /gene="fusA"
                     /locus_tag="VMZ02_05790"
     CDS             <1..1887
                     /gene="fusA"
                     /locus_tag="VMZ02_05790"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HUR71476.1"
                     /translation="FWRDHRVNIIDTPGHVDFTIEVERSLRVLDGAVAVFCSVGGVEP
                     QTETVWRQADKYGVPRLAFINKMDRVGADFFRVVKMIETRLGARPAVMQLPIGAEDKF
                     AGIVDLITMKAVVWEDESLGAKFHQEEIPADLKAQADEYREKLLEAAADADEALMEKY
                     LDGKPISEAELRAAIRKATLALKIVPIVCGTAFRNKGVQPMLDAVVDFLPSPVDIPPV
                     KGVIAGTDKPDERPARDDVPFSALAFKIMTDPFVGTLTFFRVYSGSISSGASIYNSTK
                     TKRERVGRLLKMHANKREEIKDVYAGDIAAAVGLKTATTGDTLCDEDKPIVLESIDFP
                     DPVISIAIEPKSKADQEKLGLSLQKLATEDPSFRVRTDEETGQTIISGMGELHLEIIV
                     DRLLREFSVGASVGKPQVAYRETVRKSVEQQGRFVRQTGGRGQYGDVWIKLEPQQPGG
                     GFEFVDAVKGGSIPREYIPAVEKGIREALDNGVLAGYPVVDVKVTLFDGSYHDVDSSE
                     IAFKIAGSMAFKQAARKASPVLLEPIMSVEVVVPEDFMGDVIGDLSSRRGKVLGMDTR
                     PAAQAIDARVPLAQMFGYATDVRSMTQGRATYTMQFSHYEPVPAAVAEGIIAKFDGKS
                     DDDV"
     gene            1884..2192
                     /gene="rpsJ"
                     /locus_tag="VMZ02_05795"
     CDS             1884..2192
                     /gene="rpsJ"
                     /locus_tag="VMZ02_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HUR71477.1"
                     /translation="MNEKIRIRLKAYDHRVLDQSVGEIVETVRRTGGRVAGPIPLPTR
                     IERFTVNRSPHVDKKSRDQYEIRTHKRLLDILEPTQQTIDALGKLDIAAGVDVEIKLQ
                     "
     gene            2243..2875
                     /gene="rplC"
                     /locus_tag="VMZ02_05800"
     CDS             2243..2875
                     /gene="rplC"
                     /locus_tag="VMZ02_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HUR71478.1"
                     /translation="MTGLIGRKIGMTQCYSAEGNVVPVTVVETGPCVVVQKKEPAKDG
                     YSALQVGFGSKKLQRVNKPEQGHMGKAGKGAFEVLREFRLDDVSAYEVGQEIKATDLF
                     KVGDLIDVAGVSKGHGFTGVIKRWSFAGFPGSHGTHEYFRHGGSIGNRSYPGRVRKGK
                     KMAGHWGNEQIATQNLLVIEIRAEENLMFVRGAVPGAKQGVVILRPAVKR"
     gene            2881..3477
                     /gene="rplD"
                     /locus_tag="VMZ02_05805"
     CDS             2881..3477
                     /gene="rplD"
                     /locus_tag="VMZ02_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HUR71479.1"
                     /translation="MELHVIQPTLNEAIFGVKTRPHLLHQTVVMQLANRRAGTAATKT
                     KGFVSGGGKKPWRQKGTGRARSGSIRSPIWVGGGTAFGPTPRDYSYRMPRSARREALL
                     SALSLKNREGKLIVVDKFELEAAKTKIMVQALAELKVASAVIVVAHADEKIERSARNL
                     PKVKVLRVEGLNVYDLMRYEHLILTDGALKLLEERLAA"
     gene            3474..3764
                     /locus_tag="VMZ02_05810"
     CDS             3474..3764
                     /locus_tag="VMZ02_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HUR71480.1"
                     /translation="MNSPHEIIKAPLISEKGTALTESANQVLFKVRPNANKIEVKQAV
                     EQMFKVKVEQVRMARYLGKVRRIGRSMGRRPDWKKAYVTLKEGDKIDFFGVS"
     gene            3791..4618
                     /gene="rplB"
                     /locus_tag="VMZ02_05815"
     CDS             3791..4618
                     /gene="rplB"
                     /locus_tag="VMZ02_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HUR71481.1"
                     /translation="MAIRTYRPTSAGMRFRTGHSFDEVTKERPEKNLLQPNTHSGGRN
                     NTGRITAFHRGGGHKRLYRLIDFKRNKLRVPARVEAIEYDPNRSARIALLCYTDGERR
                     YILAPEKLQVGATVIAGDDNIDIQPGNSMPLKFIPVGTILHNIELKIGKGGQLVRSAG
                     LGAQLMAKEGTYALLKLPSGELRYVLAECRATVGQVGNAEQENISWGKAGRMRWLGFL
                     PGSRGIAKNPVDHPHGGGEGRSKGNHPMTPWGKPTKGYKTRKNQSTDKYIVQRRKGR"
     gene            4621..4938
                     /gene="rpsS"
                     /locus_tag="VMZ02_05820"
     CDS             4621..4938
                     /gene="rpsS"
                     /locus_tag="VMZ02_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HUR71482.1"
                     /translation="MARSVKKGPFIDEHLQKKVTAMAESRGRQVIRTWSRRSTVTPEM
                     VGLTIGVHNGRKFIPVFVSENMVGHKLGEFSPTRTFYAHSGDRKSDAKETGGAPRPQP
                     AKS"
     gene            4973..5317
                     /gene="rplV"
                     /locus_tag="VMZ02_05825"
     CDS             4973..5317
                     /gene="rplV"
                     /locus_tag="VMZ02_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HUR71483.1"
                     /translation="METRAITKFIRVSPRKVRLVVDQIRGKGVEEALNILKFIPKRSA
                     GIVAKTVRTAVANAENTQSVDVDRLFVKQAKVDEAGMWKRFTSRAQGRATRVRKRLSH
                     VTIILDESEERS"
     gene            5325..5987
                     /gene="rpsC"
                     /locus_tag="VMZ02_05830"
     CDS             5325..5987
                     /gene="rpsC"
                     /locus_tag="VMZ02_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HUR71484.1"
                     /translation="MGQKTHPKLFRLGVIESWDSKWFARHDYPKLLHEDFRIKKFLKT
                     RLYHAGISRIEIERAANKAKINIHTARPGIVIGKKGAEIEKLKEEITKLTHRETYINI
                     HEVRRPDLDAQLTSENVALQLERRVAFRRAMKESVSRALRMGAQGVRIQCAGRLGGSE
                     IARTEWYREGRVPLHTLRADVNYGTAEAHTTYGIIGVKVWIFRGEVLTAEEEQQKAMM
                     GG"
     gene            6035..6460
                     /gene="rplP"
                     /locus_tag="VMZ02_05835"
     CDS             6035..6460
                     /gene="rplP"
                     /locus_tag="VMZ02_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HUR71485.1"
                     /translation="MLEPKKVKFRKVQKGRRRGTAHRGSTLAFGDYGLKALGRGWMSA
                     REIEAARVALTRYLKRGGKVWIRIFPDKPLTKKPLETRMGKGKGATEKWVAVVQPGRI
                     LFEMEGVEAVLAKEAFRLAAHKLSIPTMMVERRGLVHGS"
     gene            6450..6653
                     /gene="rpmC"
                     /locus_tag="VMZ02_05840"
     CDS             6450..6653
                     /gene="rpmC"
                     /locus_tag="VMZ02_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HUR71486.1"
                     /translation="MEAKELRGMSAADLTEKLTTLREEIGHLALKRATSRLENPMKFK
                     QTKRELARVATILREKALRSEEG"
     gene            6656..6937
                     /gene="rpsQ"
                     /locus_tag="VMZ02_05845"
     CDS             6656..6937
                     /gene="rpsQ"
                     /locus_tag="VMZ02_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HUR71487.1"
                     /translation="MEGRGLQKERSGLVVSDKMAKTVVVSIERTVMHPRYKKILRRRS
                     RVKAHDETNQCRVGDRVLLVESRPLSRDKRWRVSKVLETSKSGAEATVG"
     gene            6972..7340
                     /gene="rplN"
                     /locus_tag="VMZ02_05850"
     CDS             6972..7340
                     /gene="rplN"
                     /locus_tag="VMZ02_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HUR71488.1"
                     /translation="MIQTNSNLDVADNSGARRVQCIKVLGGSKRRYASVGDIIVVAVK
                     EAIPNGKVKKGDVVKAVVVRTAKELGRPDGTYIRFDNNSAVLIDNQKEPVGTRIFGPV
                     ARELRAKKFMKIISLAPEVL"
     gene            7365..7676
                     /gene="rplX"
                     /locus_tag="VMZ02_05855"
     CDS             7365..7676
                     /gene="rplX"
                     /locus_tag="VMZ02_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HUR71489.1"
                     /translation="MQIRKNDSVMVIAGKERGKTGKVLRVLPEKNALIIERISIVKRH
                     TKPRGPQQTGGIVEKEAPINVSNVMMMCDKCNAPVRIGHKDLADGRKIRICRRCNEAL
                     D"
     gene            7710..8249
                     /gene="rplE"
                     /locus_tag="VMZ02_05860"
     CDS             7710..8249
                     /gene="rplE"
                     /locus_tag="VMZ02_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HUR71490.1"
                     /translation="MARLRTKYKDDVVPAMIKEFGYKNPMQVPKLNKITINVGLGEAT
                     QNAKAIDSAVIDITAITGQKPVVTKSKKAIANFKLRENIPIGCMVTLRRERMYEFLDR
                     LIHTALPRVRDFKGVSDKSFDGRGNYSLGLREQIIFPEIQADKVDKTRGMTITIATSA
                     ATDQEGRTLLRLMGMPFAS"
     gene            8266..8451
                     /locus_tag="VMZ02_05865"
     CDS             8266..8451
                     /locus_tag="VMZ02_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HUR71491.1"
                     /translation="MAKTCLRIKAERPNKFKVREYNRCPLCGRSRAFYRRFRMCRLCL
                     RDNARKGNIPGLIKASW"
     gene            8470..>8779
                     /gene="rpsH"
                     /locus_tag="VMZ02_05870"
     CDS             8470..>8779
                     /gene="rpsH"
                     /locus_tag="VMZ02_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HUR71492.1"
                     /translation="MTDPIADLLTRIRNASRARHQKVIVPWSRLKENIVRILIDEGYL
                     KDQKKVKAAIGGGDEMIITLKFDRESRPIILGVKRVSKPGRRVYVGSQEAAPIRKGLG
                     I"
BASE COUNT         2233 a         2157 c         2554 g         1835 t
ORIGIN      
        1 ttctggcgtg accaccgggt caatattatc gatactcccg gccatgtcga tttcaccatt
       61 gaagtcgagc gttctttacg cgtgcttgac ggcgcagtgg cggtattttg ctcggtcggc
      121 ggtgtcgaac ctcaaaccga aacggtttgg cgtcaggccg ataagtatgg agttcctcgc
      181 ctcgccttta tcaataagat ggatcgcgtc ggcgccgact tcttccgagt cgtcaaaatg
      241 attgaaaccc ggctcggggc tcgtccggcg gtcatgcagc tgccgatcgg tgccgaagac
      301 aaattcgccg gtatcgtcga tctgatcaca atgaaagcgg tggtttggga agatgagagc
      361 ttgggcgcca agtttcatca agaagaaatc ccggcagatc taaaagccca agccgatgag
      421 taccgagaaa agttgctgga agctgcggcg gatgccgacg aagcattgat ggaaaagtat
      481 cttgacggca agcccattag cgaagccgag ttgcgcgcgg caatacgcaa ggccacactt
      541 gctctaaaga tcgtgccaat cgtctgtggt acggctttcc gtaacaaagg cgttcagccg
      601 atgcttgacg cagtcgtcga ttttctgccg tcccctgtcg atatcccacc ggtcaaaggc
      661 gtcatcgccg gtaccgacaa accggatgaa cgaccagccc gggacgatgt gccgttctct
      721 gcgttagctt tcaaaatcat gacggacccg ttcgttggaa ctttgacctt tttccgggtc
      781 tattctggct ccatcagttc aggggcgagc atctataact cgaccaaaac aaagcgtgaa
      841 cgagtcggcc gtttgcttaa aatgcacgcc aacaagcgtg aagaaattaa agacgtttat
      901 gccggcgata ttgcggcggc cgtaggtctg aaaaccgcga ccaccgggga cacgttatgc
      961 gatgaggaca agccaatcgt gcttgagtcc atcgattttc ccgaccctgt catctcgatc
     1021 gccatcgaac caaagagtaa agccgatcag gaaaagctcg gactgtcgtt gcaaaagctc
     1081 gccaccgagg acccttcctt tcgcgttcgc acggatgaag agaccgggca aacgatcata
     1141 tccggcatgg gcgagttgca tctcgaaatt attgttgatc gtttgctgcg cgagtttagc
     1201 gtcggtgcca gtgtcggtaa gccccaggtc gcttaccgcg agacggtacg gaaatctgtc
     1261 gagcagcaag gccgtttcgt gcgccagacc ggcggccggg gtcagtacgg cgacgtttgg
     1321 ataaaactcg aacctcagca acccggcggc ggttttgaat ttgttgacgc cgtcaagggc
     1381 ggatcgattc cccgtgaata tattccggcg gttgagaagg gtattcgcga agcgctcgac
     1441 aacggggtac tcgctgggta ccccgttgtc gatgttaaag ttacgctgtt cgacggcagc
     1501 tatcacgatg tcgattcttc ggaaattgcc ttcaagattg ccggttcgat ggcgttcaag
     1561 caggcggcgc gaaaagccag tccggtgttg ctcgaaccga tcatgtccgt ggaagtcgtc
     1621 gtaccggaag atttcatggg cgatgttatt ggcgacctaa gctcgcggcg cgggaaggta
     1681 ctcggtatgg acacccgacc ggcagcccag gcgatcgatg cgcgcgtgcc gttggcgcaa
     1741 atgtttggtt atgccacgga cgtacgttcg atgactcaag gacgggcaac gtacaccatg
     1801 cagttttctc attatgaacc tgttccagcg gcggttgcgg aaggcattat cgccaaattt
     1861 gatggcaagt cggatgatga tgtatgaacg agaaaattag aattcggctg aaagcctatg
     1921 accaccgcgt gttggaccaa tcggtgggcg aaattgttga gactgtgcgg cgcaccggcg
     1981 gacgggtggc ggggcccatt ccattgccta cgcggatcga gcggttcacg gtcaatcggt
     2041 cgccccacgt cgataagaaa tcgcgggatc agtacgaaat tcgcacgcac aagcgcttgc
     2101 tcgatatcct tgaaccgacc cagcaaacga ttgatgcatt aggtaagctg gatatagccg
     2161 ctggtgtgga tgtggagatt aagctccagt aacaaagggg aaacggagtc ggactcacca
     2221 gagcgccgat gggaatgaga tcatgactgg gctaattggc agaaaaatcg gaatgacaca
     2281 gtgctacagc gctgaaggca acgtagttcc cgtcactgtt gtggagaccg gaccgtgtgt
     2341 ggtcgtccaa aaaaaggagc ctgcgaaaga cggttacagc gcgttgcaag tcgggtttgg
     2401 cagtaagaag ttgcaacggg tcaacaaacc tgaacaaggc catatgggca aggccggtaa
     2461 gggtgctttc gaagtgttgc gggagttccg cctcgacgac gtttctgcct atgaagtggg
     2521 acaggaaatt aaggccaccg atctcttcaa agtcggtgat ttgatcgatg tcgccggtgt
     2581 atccaaggga catggcttta ccggagttat aaaacgttgg tcatttgccg gctttcccgg
     2641 gtctcatggt actcacgagt atttccgcca cggcggttcg attggtaacc gttcttatcc
     2701 cggtcgtgta cgtaaaggta aaaagatggc tggtcactgg ggtaacgaac agatcgccac
     2761 gcagaacctc ttggtcatcg aaatccgtgc cgaagaaaac ttgatgtttg tccgtggtgc
     2821 ggtacctgga gcgaagcaag gcgtagtgat cttgcgtcca gcagttaaaa ggtaattgaa
     2881 atggaactcc acgttattca accgacgctc aatgaagcca ttttcggcgt caagacccga
     2941 ccacatttgt tgcaccaaac ggtggttatg cagttggcga atcggcgtgc cggcaccgca
     3001 gcaactaaga ccaagggttt tgttagcggc ggcggtaaga aaccgtggcg tcaaaagggc
     3061 accggccgag cccgttccgg cagtattcgt tcacctattt gggtcggtgg cggcacagcc
     3121 ttcggaccga caccgcgcga ttattcgtat cgtatgccac gctcggcgcg gcgcgaagct
     3181 ctgttatcag ccctaagctt gaaaaatcgc gaaggcaagc tgatcgtcgt cgataaattt
     3241 gagctcgaag ccgccaaaac taaaatcatg gtacaagcgt tggccgagct gaaagttgcc
     3301 agtgcggtga tcgtggttgc ccacgccgac gagaagatcg aacgctccgc gcgcaatttg
     3361 cccaaagtca aagtgctgcg agtcgagggc ttaaatgttt acgatctaat gcgttatgag
     3421 cacctgatat tgaccgatgg cgcgctcaag ctgttggaag aaaggttggc agcatgaatt
     3481 cgccacatga aatcatcaaa gcgccgctga tctcggaaaa aggcacggcg cttacggaga
     3541 gcgcgaatca ggtattgttc aaggtccgcc cgaatgccaa taagatcgaa gtcaagcagg
     3601 cggtagagca aatgttcaag gtcaaggtcg aacaagtacg catggcgcgt tatctcggca
     3661 aagtgcgtcg cattggccgg agcatggggc gccgtccgga ttggaaaaag gcttacgtaa
     3721 cgctcaaaga aggcgacaag atagatttct ttggcgtctc gtaaagtgaa cgtatttagg
     3781 aacagcgaac atggcgatta gaacttaccg gccaacttcc gcgggcatgc gttttcgcac
     3841 tggccatagt ttcgacgagg taactaaaga gcggccagaa aaaaacctcc tgcaaccgaa
     3901 tacccacagc ggggggcgaa ataataccgg ccgaataacg gcctttcatc gcggtggcgg
     3961 tcataagcgg ctttatcgtt tgatcgattt caaacgcaat aagctcaggg ttccggcgcg
     4021 cgttgaagcc attgaatacg atcctaaccg atccgctcga attgccctat tatgttacac
     4081 ggacggcgag cggcgctaca tcctggcgcc ggaaaaactt caagtgggcg ccacagtcat
     4141 tgcgggcgac gacaacatcg atattcagcc cggtaactcc atgcctctta agttcattcc
     4201 cgtgggtacg atcctccaca atattgaact taaaatcggc aagggcggcc agctagttcg
     4261 gagcgctggg ttgggcgcgc agttgatggc caaggaaggt acctacgctc tattaaagct
     4321 gccttccggc gagttgcgct atgttttagc cgagtgccgc gccacggtgg gacaggtggg
     4381 caatgccgaa caggaaaata tttcctgggg taaggcgggg cgtatgcgct ggttgggctt
     4441 tttacccgga agccggggta tcgcgaaaaa cccggtcgat catccccatg gtggcggtga
     4501 aggtcgatcc aaggggaatc atcctatgac gccttggggc aaacccacca agggttataa
     4561 gacgcgaaaa aatcagtcca ccgacaagta tatcgtgcaa cgcaggaagg gtaggtagcg
     4621 atggcccgtt cggttaaaaa aggacctttc atcgacgagc atttgcagaa aaaggtcaca
     4681 gcgatggcgg aatctcgcgg tcgccaagtg attcgcactt ggtcgcggcg ctcaactgtg
     4741 acgccggaaa tggttggtct aaccatcggt gtccataatg gccggaaatt cattccggta
     4801 tttgtcagcg aaaacatggt cggccataaa ctcggcgagt tctccccgac gcggacgttt
     4861 tatgcgcatt ctggggatcg caagagcgac gctaaagaaa ccggtggggc gccgcggccg
     4921 caacctgcaa aaagctaagg caagaatacc gatcaggatg attgaccaag acatggaaac
     4981 acgcgcgatc actaaattta tccgggtctc accgcgcaaa gtgcggctgg tggtggatca
     5041 aatccgtggt aagggcgtcg aggaagcgct gaatatactc aagtttattc ccaagcgctc
     5101 agccggcatc gtcgccaaaa ccgttcgcac agcagtggcc aacgcagaga atacgcagag
     5161 cgtcgatgtc gaccggctat tcgtcaagca agctaaagtg gatgaggcgg gcatgtggaa
     5221 acgttttacg tcccgggctc aaggtcgtgc cacgagagta cgcaaaaggc tgagccatgt
     5281 gacgatcatc ctcgatgaat ccgaggagag gagttaactt ccaaatgggt caaaaaacgc
     5341 atccaaaact gtttcgcctc ggtgtcatcg agtcctggga ttccaaatgg tttgcgcgcc
     5401 atgattatcc caagctcttg catgaagatt ttcgcatcaa gaaatttctt aagacacggc
     5461 tctatcacgc cggtatctcg cgcatcgaaa tcgagcgtgc cgcgaataag gctaagatca
     5521 atattcacac cgcgcgtccc ggtatcgtca ttggcaagaa gggcgccgaa attgagaagc
     5581 tcaaagaaga aatcactaag cttacccatc gtgagaccta tattaacatt cacgaagtgc
     5641 ggcggccgga tttagacgcg caattgacct cggaaaacgt cgctttacag ctcgaacgac
     5701 gcgtcgcctt ccgtcgtgcc atgaaggaaa gtgtttcgcg cgctttgcgc atgggcgccc
     5761 aaggcgtacg cattcaatgc gccggtcgct tgggtggctc tgaaattgcc cgcaccgagt
     5821 ggtaccgcga aggtcgcgta ccgctgcata cgttgcgcgc cgacgtgaac tacggcacgg
     5881 ctgaagcgca tacgacctac ggcatcatcg gggtgaaagt ttggattttc cggggcgagg
     5941 ttttaactgc ggaagaagaa cagcagaaag cgatgatggg cggttgattg gtcccctcga
     6001 cgcgtaacac aaattcctta acagcacgaa gaaaatgctt gaaccgaaaa aagtaaagtt
     6061 tcgcaaagtg caaaagggcc gtcgccgtgg cacggcacac cgcggctcga cgctagcttt
     6121 cggtgactat ggactgaagg cattggggcg ggggtggatg agcgcacggg aaatcgaagc
     6181 ggcgcgagtc gccctgactc gatatctgaa acgcggcgga aaggtgtgga ttcgcatttt
     6241 tccagacaag ccattgacca agaagccatt ggaaacccgc atgggtaaag gaaaaggcgc
     6301 gacagaaaaa tgggtagcgg tggtccagcc gggtcggatt ttattcgaga tggaaggtgt
     6361 cgaagcggtg cttgcaaagg aagcatttcg tttggcggcg cataaactat cgataccgac
     6421 catgatggtg gaacgcagag gattagtcca tggaagctaa agaattgcgc ggcatgagtg
     6481 ccgctgacct gaccgaaaag ctcacaaccc tgcgtgaaga aatcggccac ttggcactta
     6541 agcgggcgac cagccggctg gagaatccca tgaaatttaa acagaccaag cgtgagctcg
     6601 cacgggttgc cactattttg cgtgaaaaag cgctccggag tgaggaaggc tgactatgga
     6661 aggcagagga ctacaaaaag agcgcagcgg tttggtcgtg agtgataaaa tggccaaaac
     6721 tgtcgtcgtg tcaatcgagc ggacggtgat gcaccctcgc tacaagaaaa ttttgcgccg
     6781 acgttcacgc gtgaaggcgc acgacgaaac caatcagtgt cgggtcggcg accgcgtact
     6841 gttggtcgaa agccggcctc tcagtcgtga caaacgatgg cgcgtcagca aggttttgga
     6901 aacctctaag tcgggtgccg aagcgaccgt cgggtagtct gcgcgcgagg aaagtgagtc
     6961 gaaactgatt tatgatccag accaattcca acttggatgt tgcggataat tcaggtgcac
     7021 gccgagtcca atgcatcaaa gtcttgggcg gcagcaagcg tcgatacgct tccgtcgggg
     7081 atattattgt cgtcgcggtt aaagaagcaa ttcccaacgg taaggtgaaa aaaggcgacg
     7141 tggtgaaggc ggttgtggta cggaccgcca aagagctcgg ccgcccggac ggaacttaca
     7201 tccgcttcga caataactca gcggtactga tagacaatca aaaagagccg gtgggaacgc
     7261 gtatcttcgg accggtggca cgtgaactgc gcgccaaaaa atttatgaaa atcatttctc
     7321 tagcgcccga agtgctttag ccgcagattc aggagaaatc gctcgtgcag attcgtaaaa
     7381 acgatagtgt gatggtgatc gctggcaaag aacggggcaa gaccggtaaa gtcctgcgcg
     7441 tgctgccaga aaagaatgct ctgatcatcg agaggatcag catcgtcaaa cggcacacca
     7501 aaccgcgtgg accgcagcag accggcggta tcgtcgaaaa agaagcgccg atcaatgtgt
     7561 ctaatgtcat gatgatgtgc gacaagtgta acgcgccagt tcggattggt cacaaggacc
     7621 tcgccgacgg cagaaagatt cgcatttgcc gccgttgcaa cgaagcgctc gattagtttt
     7681 gatacaacag cgggacaatt gaagtagcca tggcaagact aagaacgaaa tacaaagatg
     7741 acgtcgtccc ggcgatgatc aaagagtttg gttacaagaa tcccatgcaa gtacccaagc
     7801 tcaataaaat cacgatcaat gtcggcttgg gcgaagcgac gcagaatgcc aaggcgatcg
     7861 acagtgcggt tatcgatatc acggcgatta ctggacaaaa gccggtagtt accaagtcga
     7921 aaaaagcgat cgctaacttc aaactgcgcg agaacattcc aatcggctgc atggtcaccc
     7981 tgcggcgcga gcgcatgtat gagtttctcg accgattgat tcataccgcg ttgccccggg
     8041 tgcgtgattt caaaggagtt tcggacaaat cattcgacgg ccgtggcaat tactcgttgg
     8101 gtctgcgtga acagatcatc ttcccagaga ttcaagcgga taaagtcgac aaaaccaggg
     8161 gtatgacgat tacgatagcg acgtcggcgg cgaccgatca agaaggccgg actctgctgc
     8221 ggcttatggg tatgccgttc gccagttaat taggggggaa ttccgttggc caagacctgt
     8281 ctgagaatca aagccgagcg accgaacaaa ttcaaagtgc gggaatacaa ccgctgtcca
     8341 ttgtgcgggc gttcgcgagc attttatcgc cgttttcgca tgtgtcggtt atgtctgcgc
     8401 gataatgccc gcaaggggaa tatccctgga ctgatcaagg ccagttggta gaggttcaat
     8461 ataatgggaa tgactgatcc gatcgcggat ttactaacca ggattcgcaa cgcttcaagg
     8521 gcgcgccatc aaaaagtgat cgtgccgtgg tcgcgtttga aagaaaatat cgtcaggatt
     8581 ttgatcgatg agggttacct caaggaccaa aagaaagtca aagctgccat cggcggcggt
     8641 gatgaaatga tcatcacctt gaagtttgac cgcgaaagcc ggccgatcat cctaggggtg
     8701 aagcgagtga gtaagcccgg ccggcgggtc tatgttggct ctcaagaagc cgcgccaatc
     8761 cgtaaagggt tgggcattc
//
LOCUS       DATLCH010000196         3452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5006569, whole genome shotgun sequence.
ACCESSION   DATLCH010000196 DATLCH010000000
VERSION     DATLCH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5006569"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(782..991)
                     /locus_tag="VMZ02_05875"
     CDS             complement(782..991)
                     /locus_tag="VMZ02_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HUR71493.1"
                     /translation="MSTKTAILSIANLKSEDLPVLRDALKAVPGVGKIDFSIERSVTV
                     LEFDPALSHIDDFLRAVLKAGFQVL"
     gene            1217..1666
                     /locus_tag="VMZ02_05880"
     CDS             1217..1666
                     /locus_tag="VMZ02_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71494.1"
                     /translation="MPYEVAKLLTLSQAELDKLFTDSPVGNIPDGEAEGTAIVAPGTT
                     YSPNIARFVSHFAWQGKVFDAKKGVLKNKILPFGLNAILAKVYKAPSWLDGKECIVLD
                     YSDTSIVAQWIRDEIRELEPNVYLGKVYWDKTRLIDFALKFKRPATN"
     gene            1766..3286
                     /locus_tag="VMZ02_05885"
     CDS             1766..3286
                     /locus_tag="VMZ02_05885"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71495.1"
                     /translation="MENSRLMPASQRFFYRFGILAVIVLVLAVIVGAVFVGRFGADTP
                     VVYRDDVEHFKYGSLGSEHEFGIPYWIWRALPELFNDKLPGKKNGWQSVGFVFEKGKK
                     LPVGMSQRRYLGFDVVWLNCAFCHAGTVRDAPQSEPKVYAAMPANTFDFRGFTRFLFA
                     TGEDRRFTPQDILRQIDIIRQREGLGRLPLVDRLVLRFYGIYYMRERLLTLRDRLEFI
                     KQQPEWGPGRVDTFNPLKIYFNFPPDKLSPREVVGTTDFPSIWNQGQRENLQMNLHWD
                     GNNVSLEERNRSAAMGTGITPPTGDRQSLKRVADWLRNLDAPPYPFKIDKTLANQGAP
                     IYAKYCADCHGASGKEFNGKYAGKVIPIGEIGTDEHRLDSYTYDVAVNQNMIFAGYES
                     ERFRHFRKTFGYANSPLDGLWLRAPYLHNGSVPTLRDLLEPSDQRPKSFYRGYDVYDQ
                     KKLGFISDVAHEEDRPYFLYNTDEPGNSNKGHEGKAYGTELSTAEKDALVEYLKTF"
BASE COUNT          761 a          892 c          999 g          800 t
ORIGIN      
        1 gcctcggccc ggctggtctc acgagctgga tccagtgccg agagatatcc tatccgcgca
       61 acttttggtg acggctgtgc cgctgtgaat ggcgcaaaac ttagggaggc agtgaccagc
      121 agtaaaaaga aaaaggcccg cgccatcaat cgttctcctt tgtctttgcg aaaggtcgat
      181 ggcggacgag tgtcatcgaa gcagctgaag cgcgaaattc ggcaacgtga tctgcagttt
      241 tgcatcgtcc gtggccgaca tcatattttt atttgcggtc gcgccttgaa aatccaaaag
      301 tatggcgcgg acggaaatac aaaaaatttg ccgactcgat ccgcgcgcgt taacacgggt
      361 cggcaatcgt cagccgatct gcgctatcgc tctacctccg actccgcttt gatggtgagt
      421 tcatgcttac tctatgtggt ctcgacaacg caatcctagg accaagtatc gtgtgcggtc
      481 gctgaactga attcattgcg gattaggccg aaggcggacg atcccggcgc agccgaacag
      541 gggaggcgag tagccaggtg gctactcgct gtagtcgttt tgcgacaccg gctgcgatgt
      601 cgccgcagtt gagccgaaac ctacttcggc aagggcgcaa gcgcgctatt ggcgcgtggg
      661 ttcgtcattg cgcgcagcgg tgagtgaact cagacgagat ttctgcgttg acataaggtt
      721 attttgcttt gctgcgtggc ggaggaaact tcgaatcgag actgcggtgt tgtcgaagat
      781 gctaaagcac ttgaaagccg gccttaagca cggcgcgtaa gaaatcgtcg atgtgcgaga
      841 gggccggatc gaattcgagc acggtgacgc tgcgctctat gctgaaatcg attttgccga
      901 cgccggggac ggctttgagc gcgtcgcgca ggactggcag gtcttcggat ttgagattgg
      961 cgatggagag gattgcggtt tttgtgctca tggaaattta caatcttgtc gggactttga
     1021 caaatttgac acgaatgcac aatcggtgtg attcattcta cgatccttat tgttttaaaa
     1081 attttctttg cggcaaaatt atcttgacga attaagcgcg tgtgtgtgag agtattttat
     1141 cccaatattt agatgtgttt gtcgtgcgcg cgctttattg cgagcgcagg tgcaaacctc
     1201 agccaaggag aagatcatgc cgtatgaagt ggccaagctt ctgacgctct cgcaagccga
     1261 gctcgataag ctgtttaccg atagtccggt cggaaatatt cccgatggcg aagccgaagg
     1321 caccgcgatc gtcgccccgg ggacgacata cagccccaac atcgcgcgct tcgtcagtca
     1381 ttttgcctgg cagggaaaag tcttcgacgc taaaaagggc gtactgaaaa acaaaatcct
     1441 gccgtttggc ctcaatgcga ttctcgcgaa ggtttataaa gcgccgagtt ggctcgacgg
     1501 caaggagtgc atcgtcctcg actattcgga tacgtcaatc gtcgcccaat ggattcgcga
     1561 cgagattcgt gagctcgagc cgaatgttta tttgggaaag gtttactggg acaagactcg
     1621 gctcattgat tttgccctga aatttaagcg ccctgcgaca aactagtggg atgcaacgcg
     1681 cgctggctgg gtttcatcaa acattgcgtt gaaacgaact ataccgtggt gtgttgcggt
     1741 tgaatggatt ggaggctgac ggctgatgga gaatagccgc ttgatgccgg catcgcagcg
     1801 gtttttttat cgcttcggaa ttttagctgt tatcgtgctc gttttagctg ttatcgtcgg
     1861 tgcggtgttc gtcggccgct tcggcgccga tactccggtg gtgtaccgcg acgatgtcga
     1921 gcacttcaaa tatggctcgc ttggcagcga gcatgagttc ggtattccgt attggatctg
     1981 gcgcgccctg cctgagttgt ttaacgacaa actaccggga aagaaaaatg gctggcagtc
     2041 ggtgggcttt gtcttcgaga agggcaaaaa actgccggtc ggcatgtcgc aacggcgcta
     2101 tctcggcttc gacgtcgtgt ggctcaattg cgctttttgt cacgccggca ctgtccgcga
     2161 cgcgccgcaa agcgagccga aggtttatgc cgccatgccg gccaacactt tcgacttccg
     2221 cggcttcacg cgttttcttt ttgcgaccgg cgaggatcga cgcttcacgc cgcaggatat
     2281 tctgcgccag atcgacataa ttcgacagcg cgagggactt ggccggttgc cgctggtcga
     2341 tcgcctggtg ctgcgtttct atggcatcta ttacatgcgc gagcggctgt taacgctgcg
     2401 cgatcgcctg gagttcatca agcaacagcc ggaatgggga ccaggccgag tcgatacgtt
     2461 caatccgcta aaaatctatt tcaactttcc gccggacaaa ttatcgccgc gggaggtcgt
     2521 cggcaccacc gactttcctt cgatttggaa tcaaggccag cgggaaaatt tgcagatgaa
     2581 cctgcactgg gacggcaaca acgtttcctt agaagagcgc aatcgcagcg ccgccatggg
     2641 caccggcatt acgccgccaa ccggcgaccg gcagagtttg aagcgggtgg ccgattggtt
     2701 gcgcaatctc gacgccccgc cctatccgtt caagatcgac aaaactctcg ccaatcaggg
     2761 cgcgccgatt tacgcgaaat actgcgccga ttgtcacggc gccagcggca aggagttcaa
     2821 tggaaaatac gccggtaagg tcatcccgat cggcgagatc ggcaccgatg agcaccggct
     2881 cgactcgtat acttacgacg tggccgtgaa tcagaacatg atcttcgccg gttacgagag
     2941 cgagcgattc cgccatttcc gcaagacttt cggctatgcc aatagtccgc tcgatgggct
     3001 ctggctgcgt gcgccgtatc tgcacaacgg ctcggtgccg acgctccgcg atctgctcga
     3061 gcccagtgac cagaggccaa aaagttttta tcgcggttac gatgtctacg atcaaaaaaa
     3121 actcggcttt atttccgatg tcgcgcacga ggaggaccgg ccgtattttc tttacaacac
     3181 tgacgagccg ggcaattcca acaagggtca cgagggtaag gcgtacggca ccgaactttc
     3241 gaccgcagaa aaagacgctc tggtcgagta tctcaagact ttttgagcga cgccgggtga
     3301 gcaaggagag acgatgactg acgccgcgag gaaaaaatct gctgtgaaga tcatcttagc
     3361 gctgcttgtc gtggcgctag ccctgaccgc tgcgatcggc tggtacaagt ttttccgcga
     3421 agaaccacaa cccgcttggg tcacggcgac gc
//
LOCUS       DATLCH010000197         5955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6229467, whole genome shotgun sequence.
ACCESSION   DATLCH010000197 DATLCH010000000
VERSION     DATLCH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6229467"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VMZ02_05890"
     CDS             <1..780
                     /locus_tag="VMZ02_05890"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71496.1"
                     /translation="DPQILVLEPRAALAALTTGDLDFYTATGTSTRSALRGAPVRVLM
                     VGLNRPDHVLVVSKEITSIEQLRGKVIGGYTANATVNVLLTEMLRKKGLKSDEYKILN
                     IGTARFGALISGTVPAASLNGLETVKALRMGYRALARAADEVELATGGLGAFVGSIQS
                     KREVFRSAVQATLESIRIAATQRERVLPVLMKQFSLTQDDVGFIYDIVQKGFALDGRP
                     TPGSQKFEFDLAQKELGLKEPPRPEQVYDFSLLDEIAAAKR"
     gene            834..1661
                     /locus_tag="VMZ02_05895"
     CDS             834..1661
                     /locus_tag="VMZ02_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUR71497.1"
                     /translation="MKHKLKVYGYAIIAVLTAVTVAPFATALANDQSTLAHLLQAPYR
                     TQANVVRDAHRHPVETLTFFGVKETSTVVEILPGSRGYYLEILAPYLKNRGVYIAANR
                     DELAAPQYLADHQKFLARLKEDAEIFAKVKVTKFNADRHEIAPSGSADFVLTFRNLHN
                     WIERNEIDGALRAFHKALKPGGVLGVVDHRGRNDLSQDAQMKFGYVREDVAIKLMEKA
                     GFKLAGTSQVNNNPKDTKDYPDGVWTLPPNLRLGDRDRAKYQAIGESDRFTHRYVKQ"
     gene            1785..2621
                     /gene="purU"
                     /locus_tag="VMZ02_05900"
     CDS             1785..2621
                     /gene="purU"
                     /locus_tag="VMZ02_05900"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HUR71498.1"
                     /translation="MPPHILRIDCPDQPGLVNKITGVLFNAGYNILSNQEFVDLDAKH
                     FFMRTAFEGPAQPERVVSHLQQILPAESVVKLVEAARQPIVILATTEAHCLGDLLIRH
                     YSGELPAQIKAVIANHETLGSLVKPFAIPFHCVSHKDLERPEHEKKVMEVIDSYGPEY
                     LVLAKYMRILTPTFVARYRNRVVNIHHSFLPAFIGAKPYHQAYQRGVKIIGATAHIVT
                     DDLDTGPIIAQNVIPVNHTYTAAAMVQAGRDAEKIALARAVRLILEERVFLHGNRTVV
                     FE"
     gene            2862..4307
                     /locus_tag="VMZ02_05905"
     CDS             2862..4307
                     /locus_tag="VMZ02_05905"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR71499.1"
                     /translation="MAKDLRSFIAECERQLPGEVIHITKEVNPANYDVTAIIKHLGAQ
                     KKFPIIIFDNPLNLNGKVGPIKLTMNCEISQGKTQIALGMPRTATRPEMAEKCLEMEE
                     HRVPPTIISKKDAPIKQNTMVGDKVDLYQIPIMRHHEMDGGPYIVLSTITKDRKSGIY
                     NLSYHRMEVKSKNSTALYASPRHLWKIFKDYEDNNMEMPVATVLGHHPAFHMGACYSG
                     PFEVSEYDVIGGYMGEPLRLVASETFGDDLLIPADAEVVIEGVLKPGGRVVEGPFGEA
                     PGYLGPQRYTTCVHYEVKAINYRNGAMYQSVITPEGDKPWMDLPREGAYLRRCREAVP
                     GVTAVCKQGRHAHYNVFISMKKMSEGDPARAAAAALTFDHTKNVFVFDEDIDVFNPTD
                     ILWAIATRVQPHLQVNILKPLFRGNFLDPSLRDEIKTSGMIVDATKPTDRPFSPVSKC
                     PDEAMARIKLEDYVPGEVLQHIPMDRTTYWA"
     gene            4398..4544
                     /locus_tag="VMZ02_05910"
     CDS             4398..4544
                     /locus_tag="VMZ02_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71500.1"
                     /translation="MGQAVEITCPDCKKVFVVNPHMLGSGMNFHCPYCDKYFPEKDSP
                     KIRK"
     gene            4607..>5955
                     /locus_tag="VMZ02_05915"
     CDS             4607..>5955
                     /locus_tag="VMZ02_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUR71501.1"
                     /translation="MKTMQNFKVVGTNAHRVDAVEKVSGKAIYTSDIQLPGMAHARIL
                     RSPVAHAKLLKVDASKAKEMPGVIATLTRDDIQRFNFKYGATYKDQSIVAVDKVRYVG
                     DPVAAVLADTPEIAEQALELIEVEYEELPKVTNLEEATAAGAIQVHEGGVARAELRGS
                     TYGAPERFNGTNICYYLTFGREDVEKGFAKADHIFEDTFRFQKVQHYSLEAHNNVAYF
                     DGEKLTVWASCQDPFTLRDHLSGIFRLPCNRIRVIVPYVGGGYGGKLYVKAEPISAAL
                     TWMTRRPVKLQLSINESFKTVTRHPARVTVKTGVSKDGTLVARECQVYMDTGAYADAG
                     PRVTQKAGYRALGPYRIPYAKVEAHGVYTNTVPAGAFRGFGALQVTWAYESQMDMIAD
                     RLGLDPLEFRIKNLLKKGDLYTVGDTPVDCDMEEGLRKIADAIKWSEKSPTPNRAKG"
BASE COUNT         1420 a         1621 c         1654 g         1260 t
ORIGIN      
        1 gatccgcaga tactagttct cgaacctcgg gcggcgcttg cggctctaac gaccggcgac
       61 ttggattttt atacggcaac cgggacgtcg acgcgttcgg cgctgcgcgg cgcgccagtg
      121 cgcgtactca tggtcgggct gaatcggcca gatcatgtgc tggtggtttc aaaggaaatc
      181 accagcatcg agcaactgcg tggcaaagtc atcggcggtt acaccgccaa cgctacggtg
      241 aatgttttgt tgacggaaat gctgcgcaag aaggggctca agagcgacga gtacaaaatt
      301 ctgaatatcg gcacggcgcg cttcggcgcg ttgatcagtg gcacggtgcc ggcggcatct
      361 ctcaatggtc tcgagactgt caaagcgctg cgcatgggtt accgagcgct ggcgcgtgcc
      421 gccgatgagg tagagctcgc gaccggcggg ctcggcgcct ttgtcggcag tattcaaagc
      481 aagcgcgagg tattccgctc ggccgtgcag gcgactttgg agagcattcg catcgccgct
      541 acccagcgcg aacgggtttt accggtattg atgaagcaat tttctctgac ccaggatgat
      601 gttggcttca tctacgatat agtgcaaaaa ggctttgcct tggatggcag gccgactccg
      661 ggcagtcaga agttcgagtt cgatctggcg cagaaagagc ttggcttgaa agagccgcca
      721 cggcccgagc aagtttatga tttctcattg ctcgatgaga tcgccgcggc taagagatag
      781 gagtagggct ttggattaat agcggggaaa gcgcgttaga accggagaat aacatgaagc
      841 acaaattaaa agtttatggg tacgcaatca tagccgtttt gacagcagtg accgtagcac
      901 cctttgcaac tgcactggcc aacgatcaaa gcacactcgc gcatttattg caggctccct
      961 atcgtactca agccaatgtc gtacgcgatg ctcaccgtca cccggtggaa acactgacgt
     1021 tctttggcgt caaagaaacc agcacggtag tggaaatttt gccgggtagc cgtggctact
     1081 atttggaaat acttgctcct tacttgaaga accgcggcgt ttatatcgcc gccaatcgcg
     1141 acgagttggc ggcgccgcaa tatcttgccg atcatcagaa gtttctcgcg cggctcaagg
     1201 aagacgcgga gattttcgcc aaggtaaaag tcaccaaatt caacgccgac cgccatgaaa
     1261 tcgcgccgtc gggtagcgcc gactttgttc tgacctttcg caatttgcac aattggatcg
     1321 agcgcaatga gatcgatggc gcgctgcggg cgtttcacaa agccctcaaa ccgggcgggg
     1381 tgttgggtgt ggtcgaccac cggggccgca acgacctttc ccaggacgcg caaatgaaat
     1441 tcggctatgt ccgcgaagac gtcgcaataa agttaatgga aaaagccggc ttcaaactgg
     1501 ccggaacttc gcaggtcaac aataatccaa aggataccaa agattatccc gacggtgtct
     1561 ggaccttgcc gccgaacttg cgtttgggtg acagagatcg cgcgaagtac caggcgatcg
     1621 gcgagagcga tcgcttcacg caccgctacg taaagcaata actgcaaagc aggccaggga
     1681 aattctgcaa cgctccgaag ggcggcggct gatccgtcac tggtttatct acggtcgcgc
     1741 gcatgcgata cttggcgcga attatcgtcc aaggagagcc atccgtgccg ccacatattt
     1801 tacgcatcga ttgccccgat caacctgggt tggttaacaa gatcaccggt gtcctgttca
     1861 atgccgggta taatatttta agcaatcagg aatttgtcga tctcgatgcc aagcatttct
     1921 tcatgcgcac ggctttcgag gggccggcgc aaccggaacg ggtggtttct cacctccaac
     1981 agatcttacc cgcagagtcg gtggttaaac tggtcgaagc ggcgcgccag ccgattgtca
     2041 tcctggcgac caccgaagcc cattgtctag gcgatctgct gatccgccat tactccggcg
     2101 aactgccggc gcaaatcaaa gcggtgattg ccaaccatga gaccttgggc tcattggtca
     2161 agccatttgc catcccgttt cactgcgtta gtcacaagga tctcgaacgg cccgaacacg
     2221 agaaaaaggt gatggaggta atcgattcct acgggcccga gtacctggtg ctggccaaat
     2281 acatgcgcat tcttacgccc acgttcgtgg cacgttatcg caaccgcgtt gtcaatattc
     2341 accattcttt tttgccggca ttcatcggcg ccaaaccgta tcatcaggcc tatcagcgcg
     2401 gcgtcaagat catcggcgct acggctcaca tcgttaccga cgatctcgac acgggaccta
     2461 tcatcgcgca aaacgtcatt cccgtgaacc acacctacac tgcggcagcg atggttcagg
     2521 ccggcaggga cgcggaaaaa atcgctctcg ctcgcgcggt gcggctgata ctcgaagaac
     2581 gagtgtttct ccatggcaat cgcacggtgg ttttcgaata gttcccagaa tctctactga
     2641 acctgcgcac gactggcgaa tcgacgtctg atagtccgtc ggtagtgcag catttctttc
     2701 gcgacacaaa gtgagcccac ttggcagcac tcagttgcgg aaaaatcgca gctaaatcaa
     2761 gggctcatac gctatggttc ggcaacttga tttgacaatg ccagggccat ccgataaatc
     2821 tttttgcata aaatttttca taacttagct aggaggccta catggcgaag gatctaagga
     2881 gtttcattgc cgagtgtgaa cggcaattgc ccggagaggt gattcatatc accaaggaag
     2941 tcaatccggc gaattacgat gtaaccgcaa tcatcaaaca tcttggcgcc cagaagaagt
     3001 ttccaatcat tattttcgac aacccgctta acttgaacgg taaagtcggc cccatcaagc
     3061 tgaccatgaa ctgcgagata tcgcagggta aaacgcagat cgccttgggc atgccgcgca
     3121 cggctactcg ccccgaaatg gcggaaaaat gcctggaaat ggaagagcac cgggtgccgc
     3181 cgacgatcat cagcaagaag gacgcgccca tcaaacaaaa caccatggtc ggcgacaaag
     3241 tcgatctcta ccagatcccg atcatgcgtc atcacgaaat ggacggcggt ccgtacatcg
     3301 tcctctcgac catcaccaag gatcgcaagt cgggcattta caatctgtcc tatcatcgga
     3361 tggaagtgaa atcgaaaaac tccacggcgc tctacgcttc gccgcgtcat ctgtggaaga
     3421 tttttaaaga ctacgaagac aacaatatgg aaatgccggt ggcaaccgta cttggccacc
     3481 atccggcatt ccacatgggc gcttgctata gcggcccgtt cgaagtcagc gagtatgacg
     3541 tgatcggcgg ttacatgggc gaaccgctgc gcttggtcgc atcggaaacg ttcggcgatg
     3601 acttgctgat cccggccgat gccgaagtag ttatcgaggg cgtgctcaaa ccaggcggcc
     3661 gcgttgtcga aggaccgttc ggcgaagcgc cgggctatct tggcccgcag cgctacacca
     3721 cctgtgtgca ctacgaagtg aaggccatta actaccgcaa cggcgccatg tatcagtcag
     3781 tcatcacgcc ggaaggtgac aaaccctgga tggatctgcc gcgcgaaggc gcctatctac
     3841 ggcgctgccg tgaggcggtt cccggcgtca ccgccgtctg taaacaaggc cgccacgcgc
     3901 attacaatgt tttcatctcg atgaagaaga tgtcggaggg cgatccggcg cgcgccgccg
     3961 ccgccgcgtt gaccttcgac cacactaaga atgtttttgt gttcgatgag gatatcgatg
     4021 ttttcaatcc gaccgatatt ctttgggcga tcgcgacccg ggtgcagccg cacctgcaag
     4081 tcaatatttt aaagccgctg ttccgcggca atttcttgga tccgtcgctg cgcgatgaga
     4141 tcaagacttc tggcatgatt gtcgatgcga ccaaaccgac cgatcggccg ttctcgccgg
     4201 tgtcgaaatg tcccgacgaa gcgatggcgc ggattaagct ggaagactac gttcccggtg
     4261 aagtgttgca acatatcccg atggaccgga cgacctactg ggcgtaatgc aaccccacct
     4321 tgttcctccc gtgcacgggg agcgattaag agggattcag agaatcctaa gagctaaaat
     4381 agagagagag gtaaaccatg ggtcaggccg ttgaaattac ttgtccagat tgtaagaaag
     4441 ttttcgttgt aaacccgcat atgctcggct ccgggatgaa ttttcattgc ccttactgcg
     4501 acaaatactt ccccgagaaa gacagcccga aaatccgcaa ataacttcgc tgccaggcca
     4561 cggtgacaaa atcatcgtgg cctttttcat tttttacgct cctatcatga agacgatgca
     4621 aaattttaaa gtcgtcggca ccaatgcgca tcgtgtcgat gccgtcgaaa aggtcagcgg
     4681 taaagcgatc tacaccagcg acattcagct gccgggcatg gcccatgcgc ggatactgcg
     4741 cagtccggtg gcgcatgcga aattgctaaa agtcgatgca tcgaaagcca aggaaatgcc
     4801 cggtgttatc gcgaccttga cgcgcgacga tatccaacgg ttcaacttca aatatggcgc
     4861 gacttacaag gaccaatcga tcgtcgcggt cgacaaggtc cgctacgtcg gcgatccggt
     4921 cgctgcggta ctggccgata cgccggagat cgccgagcag gcgctggagt tgatcgaagt
     4981 cgaatatgag gagctgccca aagtcaccaa tcttgaagaa gccacggccg caggcgctat
     5041 tcaggtgcac gaggggggcg tggcacgcgc cgagctgcgc ggttcaacgt acggcgcgcc
     5101 ggagcgcttt aacggcacca acatttgcta ttacctgacc ttcgggcgtg aagacgtcga
     5161 aaagggcttt gccaaagcag atcatatatt cgaggacacc ttccgctttc aaaaggtcca
     5221 gcactattcc ctcgaggcgc acaacaacgt cgcttacttt gacggcgaga agctaacggt
     5281 ctgggcgtca tgccaggatc cgtttacgct gcgcgatcat ttgtcgggta tttttcgtct
     5341 gccgtgcaac cgcattcgcg tcatcgtgcc ctatgtcggc ggtggttacg gcggcaagct
     5401 ctatgtgaag gcggagccga tctccgccgc gttgacctgg atgacccggc ggccggtgaa
     5461 actgcagctg tcgatcaatg aaagttttaa gactgtcacg cgccatccag ccagagtcac
     5521 ggtcaaaacg ggtgtgagta aggacggcac gctcgtcgct cgtgaatgcc aggtttatat
     5581 ggacaccggc gcctacgccg atgccgggcc gcgggttacc cagaaggccg gttatcgcgc
     5641 tttgggacct tacagaattc cctatgccaa ggtggaagca cacggcgtct ataccaacac
     5701 agtgccagca ggcgcattcc gtggcttcgg agcattgcaa gttacctggg cctacgagtc
     5761 gcagatggac atgattgccg atcgcttggg tctcgacccg ctcgagtttc gcatcaagaa
     5821 tttattgaag aagggcgatc tctacacggt cggagacacg ccggtggact gcgacatgga
     5881 agagggactt cgcaagatag ccgatgcgat aaagtggagt gaaaaaagcc caacgcccaa
     5941 tcgcgccaag ggaat
//
LOCUS       DATLCH010000198         4365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2775037, whole genome shotgun sequence.
ACCESSION   DATLCH010000198 DATLCH010000000
VERSION     DATLCH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2775037"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VMZ02_05920"
     CDS             <1..923
                     /locus_tag="VMZ02_05920"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUR71502.1"
                     /translation="TVLPYYRRFITALPSVQSLARARLQRVLRLWSGLGYYRRAENLH
                     QAARQIMRQHSGVMPRDFDHLRALPGIGDYTAGALLSIAFGKKYPAIDGNVRRVLSRL
                     LLTKSHEKIHSYAAKLAKTSDPGNLNQALMELGAMICIPNQPQCDNCPVAPDCRSRQR
                     LGSRLEKIPTAKMTFRDVTWPLAIVSYRGKILLRRRRADGLLAKLWELPGAEINAHEK
                     PEGVVTRELKPLAVTGLVPRRLGELRHSITNRRIHAPVLLYRWPMNQPGPEIKAEWHW
                     VVPNELQRYATSSMTTKAVKLFLQHEKNSA"
     gene            904..1665
                     /locus_tag="VMZ02_05925"
     CDS             904..1665
                     /locus_tag="VMZ02_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HUR71503.1"
                     /translation="MKRILLSSFLATLLVSGAYYFYFSLPDVSLLKQKNPATTALMEL
                     RDREYQQQNSRPRRQQIWVSYGAVSEHVKKAILVSEDAAFFSHKGVDVTELKAALKKD
                     LETLSFSRGGSTLTMQLAKNLYLNPSKNPLRKFKEIIIAHQLENALSKRRIFEIYLNI
                     VEMGRNVYGVEAAAQHYFGKSVAAVDLVEAATLAALLPSPRNSKQRSLAARRNIILNR
                     LASVGYLSPSENRQAQELPPAKKAEEADVELPAND"
     gene            1907..2119
                     /locus_tag="VMZ02_05930"
     CDS             1907..2119
                     /locus_tag="VMZ02_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71504.1"
                     /translation="MEKPSNRRIDTTLGDLIAAASEVAFEYSDNDKQAYKLAQVALME
                     LIKKSAHPIDFTDEFETLSTSSSVIH"
     gene            2532..2837
                     /locus_tag="VMZ02_05935"
     CDS             2532..2837
                     /locus_tag="VMZ02_05935"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HUR71505.1"
                     /translation="MIPQLRVWHDNYAPAGLTIVGVHSPEFPWEKSLDRVKDATSRLG
                     IKFPVVQDNNFAIWQRYGVRAWPTIVMVDKMGIIRYSHIGEGAYGKTESMIKRLIAE"
     gene            2866..3831
                     /gene="msrP"
                     /locus_tag="VMZ02_05940"
     CDS             2866..3831
                     /gene="msrP"
                     /locus_tag="VMZ02_05940"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HUR71506.1"
                     /translation="MLIKKPADIKAAEITPKEHYLNRRQFMLSASALALSTGAFLGGV
                     DIGENFGNVFGAEKLANVKKSSFSTNEKLNSLKEITNYNNFYELGTDKEDPAANAKYL
                     TPTRPWAVSVEGEVKKAKTYDIEELIKLAPLEERIYRLRCVEAWSMVIPWIGFPLSSL
                     IKLAEPTGNAKFIQFVTLNDPKRLPGQKIPTLEWPYVEGLRMDEAMHPLTILTVGLYG
                     EVLPAQNGAPIRLVVPWKYGFKSIKSIVKVRFVEKQPAASWNTMQPQEYGFYSNVNPE
                     VDHPRWTQATERRIGEFRRRKTLMFNGYAEQVASLYSGMDLKKFY"
     gene            3958..>4365
                     /locus_tag="VMZ02_05945"
     CDS             3958..>4365
                     /locus_tag="VMZ02_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HUR71507.1"
                     /translation="MLSYLKPLLFIACLVPLAQLGFSYYNDALTANPIEYITRFTGSW
                     ALLILLASLAVTPLRKLTGWNELIKSRRMLGLFAFFYAALHFATYMVLDLFFDFAAIG
                     KDILKRPYITVGFSALVLMIPLALTSNAAMIRRL"
BASE COUNT         1042 a         1265 c         1127 g          931 t
ORIGIN      
        1 aaaccgtgct gccgtactat cggcgcttca ttacggcatt acctagcgtt cagtcgctcg
       61 cgcgcgcgcg attgcaacga gttctgcgct tgtggtcggg gctaggctac taccggcgcg
      121 ccgagaatct gcatcaagcc gcgcgccaga tcatgcgcca gcatagcggc gtgatgccgc
      181 gcgatttcga tcacctgcgc gccttgccgg gtatcggcga ctacaccgcc ggcgcgctgc
      241 tgagcatcgc ctttggcaag aaatatcccg ccattgacgg caacgtgcgg cgcgtgctga
      301 gccgtctgtt gctgacaaaa tcacatgaaa agattcattc ttatgccgct aaactagcga
      361 aaacatccga tccagggaat ctcaaccaag cgttgatgga actcggcgca atgatctgca
      421 tacccaacca accgcagtgt gacaattgcc ccgtggcacc cgactgccgc agccggcagc
      481 ggctgggcag tcgattggaa aaaatcccaa cggcaaaaat gactttccgc gacgtcactt
      541 ggccgctggc catcgtctcg tatcgaggga agattctcct gcggcgccgg cgagccgatg
      601 gcttactggc caaattgtgg gaacttccgg gtgctgagat taatgcgcat gaaaaaccgg
      661 aaggcgttgt cacgagagaa ctaaaaccgc ttgcggtcac cggcctggtg ccgcgccgct
      721 tgggcgagct gcgccatagc attaccaatc ggcgcatcca tgcgccggtg ctgctttacc
      781 gttggccgat gaaccaacct gggcccgaaa tcaaagccga gtggcattgg gtcgtaccaa
      841 acgagctgca acgctatgcg acgtcgtcga tgaccaccaa ggcagtaaag ttatttctcc
      901 aacatgaaaa gaattctgct tagctcgttt ttggcaaccc tcctggtcag cggcgcgtat
      961 tacttttatt tctcgttgcc cgacgtttcg ctgctcaagc agaagaaccc cgccaccaca
     1021 gcgctgatgg aactgcgcga ccgcgagtac cagcagcaaa acagtcgccc ccggcgccag
     1081 caaatctggg tatcctacgg cgccgtatcc gagcacgtca aaaaggcgat tctcgtcagc
     1141 gaagacgcgg cgttcttcag ccacaagggc gtcgacgtaa ccgagctcaa agccgcactc
     1201 aagaaggatc tggagactct cagcttcagc cgcggcggca gcacgctcac catgcaactc
     1261 gccaagaatc tctatctaaa tccgtcgaaa aatccgctgc gcaaattcaa ggaaattatt
     1321 atcgcgcacc agttggaaaa cgctttgtcc aaacggcgta tctttgaaat ttatttgaat
     1381 atcgtcgaga tggggcgcaa cgtttatggt gtcgaagcgg cggcgcaaca ctactttggc
     1441 aagtccgtcg cggcggtcga tctggtcgaa gcggcgacac tcgccgccct gctgccgagc
     1501 ccgcgcaatt ccaaacagcg cagcctggcg gcgcgccgca atatcatatt gaaccgcctc
     1561 gccagcgtcg gctatctgag cccgagcgaa aatcgacagg ctcaagagct ccccccggcg
     1621 aagaaggctg aagaagctga tgttgaattg ccggcaaacg attgagtgtc accttgcgaa
     1681 tacttgatca ataggcgacg ctaaacgata gttggtatct ttttctagca ggaaaaatcc
     1741 ccgccgttct aaacggagcc tcttccggcc tgatcggctc ggcatccagg atgttgtcag
     1801 accgaacacg gattccgctt tgacgactga gcaatacgcc gcagcagtac aacctttttt
     1861 ccaccctatc gtctaaataa acgagggaag aaaggagcca ccagaaatgg aaaaacccag
     1921 caaccgcaga atcgacacga ccttgggaga cttgatcgcc gccgccagtg aagttgcctt
     1981 cgagtattcc gacaacgaca aacaggccta taagctggct caagtcgcgc tcatggaatt
     2041 aatcaagaaa tcagctcatc cgatcgattt tactgacgaa ttcgaaactc tctcaacctc
     2101 ctcctcagtc atccactagc catcccgccg gcgcgccgga gcccaccgcg gctccggccg
     2161 cgcagttaaa gggcacattc acagcttggg gaactttggc cgacgagctg aatagcaaaa
     2221 ttagttgcaa ctgctgccgt ccaaatgtca tctaattagc tagcgaatgc agacattcgg
     2281 atttattccg aagctggctg aggagatcgc aaaatatggt acgcctcgcg gctgttacaa
     2341 cggctctcgc tttgacacta gtgttggggg cggcttggcc gcgcaccagc acggcactga
     2401 ccagtggaag accggcagcg gatttcgcgg ctgataactg gatcaacagc acaccgctca
     2461 ccgtcgcgag cctcaggggc cgagtagtcc tagtcgagtt ttggacttat ggctgaatca
     2521 actgtagaaa catcatcccg cagttgcggg tttggcatga caattacgcg ccggccggat
     2581 tgaccatcgt cggtgtccac tcgccggaat ttccctggga gaaatctttg gatcgggtga
     2641 aagacgcgac ctcgcgtttg ggcattaaat ttcccgtggt acaagacaat aacttcgcca
     2701 tttggcagcg ctacggcgtc cgggcctggc ctactattgt gatggtcgat aagatgggca
     2761 taatccgtta cagccatatt ggtgaaggcg cctacggcaa aaccgagtcg atgatcaaac
     2821 gactcatcgc cgaataactg cgcaaagata ttcgaaggag attccatgct catcaaaaag
     2881 cccgccgata tcaaagcagc ggagatcacg cccaaagagc actacttgaa ccgccggcag
     2941 ttcatgcttt cggcgtctgc cctggcgctc agcactggag catttcttgg cggcgtagac
     3001 atcggtgaaa acttcggcaa tgtcttcggc gccgaaaaat tggccaacgt caagaaaagt
     3061 tcgttcagca ccaacgaaaa actcaactcg ctaaaagaaa tcaccaacta caataatttt
     3121 tatgaactcg gcacggacaa ggaagatccg gcagccaacg caaagtatct cacccccacc
     3181 cgtccgtggg cagtttcggt cgagggcgag gttaaaaaag ccaaaactta cgacattgaa
     3241 gagctgatta aattagcgcc attggaagag cgaatctatc gcctgcgttg cgtcgaggcc
     3301 tggtcgatgg taattccgtg gatcggcttt cctctaagtt cgctgatcaa gctggccgag
     3361 cccaccggca atgccaagtt catccagttc gtcaccctca acgatccaaa acgcctcccc
     3421 gggcagaaga taccgacact ggaatggccc tatgtcgagg gcttgcgtat ggatgaggcg
     3481 atgcacccgc tgacgattct tactgtggga ctctacggtg aggtcttgcc ggcgcagaac
     3541 ggcgcgccga ttcgtctcgt ggtgccgtgg aaatacggtt tcaagagcat caaatccatc
     3601 gtcaaagtcc gcttcgtcga gaaacaaccg gctgcttcgt ggaacacgat gcaaccgcag
     3661 gaatacggct tctactctaa cgtgaatccc gaagtcgatc acccgcgctg gacccaagcc
     3721 acggaacggc gtatcggcga atttcgccgg cgcaaaaccc tgatgttcaa cggctacgct
     3781 gagcaagtgg caagcttgta cagcggcatg gacctgaaga agttctatta agccaacgga
     3841 gaatggctaa ttgacaattg ataatgtcag aacggatcca tagctgaatt tctcgggcac
     3901 ggccgttctt tcattctcca ttctccattc tcaattttcc attatcgatt ccttccaatg
     3961 ctctcctacc tgaaaccgct gttattcatc gcctgcttgg tgcccctcgc ccagcttggc
     4021 ttcagctatt acaacgacgc tctcacggcc aatccgatcg agtacattac ccggtttacc
     4081 ggctcctggg ccttattaat tctactcgcc agtctcgccg tcactccgct gcgcaagctc
     4141 accggctgga acgagctgat taaatcgcgc cgcatgctcg gcttgttcgc gtttttctac
     4201 gccgcgctgc acttcgccac gtacatggtg ctcgatctat ttttcgattt tgccgccatt
     4261 ggcaaggata ttttgaagcg gccgtatatc accgtcggtt ttagcgcgtt ggtattgatg
     4321 atcccgctgg cgctcacctc gaacgcagcg atgatccgcc gcctc
//
LOCUS       DATLCH010000199         4719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1286905, whole genome shotgun sequence.
ACCESSION   DATLCH010000199 DATLCH010000000
VERSION     DATLCH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286905"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VMZ02_05950"
     CDS             complement(<1..775)
                     /locus_tag="VMZ02_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR71508.1"
                     /translation="MDETPIVQVDQVSKKYCRRLRHSLWYGLVDLGAELAAHNGNGEL
                     RLRRAEFLAVDNVSFELRPGECVGLIGHNGAGKSTLLKMLNGLVRPDKGKITMRGRVG
                     ALIELGAGFSPILTGRENIYINGAVLGFSKSEIDAKFDEIVDFAELDDAIDAPVQTYS
                     SGMYVRLGFAVAAQLNPDIFLIDELLAVGDTGFRMKCFQHLLEMKDAGKSIVVVAHNM
                     IDINRVCDRVVVLESGKKIYDGDVSSGIATYESLASKRGH"
     gene            complement(778..1641)
                     /locus_tag="VMZ02_05955"
     CDS             complement(778..1641)
                     /locus_tag="VMZ02_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR71509.1"
                     /translation="MNQRANSQTICEYTPASQLTDPARLFRNMICDIAASRELAWRLL
                     ARNLSSRYRHTALGYIWAFLPPVLSTTVFVFLRQSGYFTVGQTAAPYALFLFAGLILW
                     QVFCDAIGMPLRMVQQSHTILTKVNFPREALVIAGVGEVLFGFVIRFALLLVGLWWFN
                     IGVSLSMFLVPFGVIVLIGLGVGLGLVLTPIGILYHDVGQGLPFVLYLWMFLTPVLYP
                     APQSWPAWGAWILNPVGPILDTTRAWLLSVPPEHWAGFQIASVLAGSTLLIGWLLYRL
                     ALPILLERMSA"
     gene            1798..3039
                     /locus_tag="VMZ02_05960"
     CDS             1798..3039
                     /locus_tag="VMZ02_05960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUR71510.1"
                     /translation="MSRPPSLKILFLTSSYPRHEHDVASVFLHYFASSLVARGNQVHV
                     LAPAEGKDGVAADGAITVHRFQYFPQRWQKLAYGSGIMPNLSRSPLLWLQVPFYVLSM
                     ILALVRLMRQEKFDVLHAHWILPQGLIAIVAKSIQPIPLVTTAHGADAFALNGRLNGF
                     IKRLVISKSDAWTANTEATCAAVETNYSRTSARIIPMGVEIERFSNGDRSALRGLVDQ
                     SEFLLLFVGRLVEKKGVDDLLRALALLPAAFRSRTRLWIIGEGDLRSTLEKTAVDCAI
                     DQQVRFWGVVRNQDLPDYYAAADLFIAPSIEAKSGDTEGQGVVLLEAFAARLCVIATR
                     CGGIDAVVRDQHTGILVPPNDPERIAAAIETLITDTALRTRLADNAFAEVRERYDWRY
                     IAGEFDKLYRQLVSSAAFVTD"
     gene            complement(3052..3939)
                     /locus_tag="VMZ02_05965"
     CDS             complement(3052..3939)
                     /locus_tag="VMZ02_05965"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUR71511.1"
                     /translation="MANPVETSVIIPIFNEAENLKKLIGRIRAIELPDSEILVVDDGS
                     CDGSAEIAMAAGANVVRHPYNIGNGAAIKSGMRAAKGKFIVLMDGDGQHKPEDIPKLL
                     ADADVFHMVVGARAKGSRLRMHRNLANMVYNLLASYVTHFKVEDLTSGFRAMRRRDAL
                     RFIDLLPNTFSYPTTLTLAFLRSGLTIKYVPIQTLYRAGQSKISLVTDGIRFLLIITK
                     IATLFAPFRIFLPVSVFFFLGGIGNYIYTYVTQNRFTNMSVFALTTSVIIFMLGLISE
                     QIALLRMERQDAGRRHDDG"
     gene            complement(3932..>4719)
                     /locus_tag="VMZ02_05970"
     CDS             complement(3932..>4719)
                     /locus_tag="VMZ02_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71512.1"
                     /translation="DLWHIVALPLRLFFSGRDDNPQFFDGVLTPILIVLLPWAFRGKW
                     CEDKKLLAGFFLLTLLYGIFLVDLRVRYVLPMVPPLIILLVYGVFNIYTRIKRPVILF
                     AGLFVFAAWHGVYLWKYIAEAAPWPYLIGEESRDDYLSRTLPEYSTFRFINRETPANA
                     RIYLLFVGRRAYYCQRNYFHDGADLPGFLLGAIRNAKNTEQLAQSFRQKQITHLMTRE
                     DLLSRFLNNNLTPNQARLWNEFTANRLRLSFRESGHAVYQLNG"
BASE COUNT         1176 a         1318 c         1239 g          986 t
ORIGIN      
        1 tgtgaccacg ctttgacgca agactttcat aggttgcgat ccccgacgac acatcgccgt
       61 cgtaaatttt tttgccgctc tctaacacca cgacccggtc gcacacgcgg ttgatgtcga
      121 tcatgttatg cgcgacgacc acgattgact tgccggcgtc tttcatctcg agcaagtgct
      181 gaaaacattt catgcgaaac ccagtgtcgc cgaccgccag cagttcatcg atcaaaaaga
      241 tatccgggtt caactgcgcc gccacggcaa agccgagccg aacgtacatg ccggagctgt
      301 aggtttgcac cggcgcgtcg atggcgtcgt cgagctcggc gaagtcgacg atctcgtcga
      361 acttggcgtc gatctcgctc ttagaaaacc cgagcactgc tccgttgatg taaatattct
      421 cgcgtccggt gagaatcggg ctgaaaccgg cgccaagctc gatcagggca ccgacgcggc
      481 cgcgcatggt gattttccct ttgtccggac gaaccagacc gtttagcatt ttaagcagcg
      541 tgctcttgcc cgcgccgtta tggccgatca gaccgacgca ctcgcccggc cgtagctcga
      601 acgacacatt atcgacggcg agaaactcag cgcggcgcag gcgtaattcg ccgttgccat
      661 tgtgcgccgc caattccgcc ccaagatcga ccagaccgta ccacaaggaa tgacgcaggc
      721 ggcggcaata tttcttcgag acctgatcga cttggacgat tggagtctca tccatagcta
      781 ggcgctcatc cgctctagca agatgggcag agccagacga taaagcagcc agccgatgag
      841 aagagtagaa cccgccaaga ctgaggcgat ctgaaatccc gcccaatgtt ccggcggcac
      901 ggataatagc caagccctcg tggtatccaa gatgggaccg acggggttca atatccaagc
      961 cccccaagct ggccaggatt gcggcgcggg atagagaacc ggcgtaagaa acatccaaag
     1021 gtataggaca aacggcaacc cctgtccgac atcatggtaa agaataccga tgggcgtgag
     1081 cacgaggcca aggcccacac ctaaaccgat gaggacgatt acaccgaacg gaaccaaaaa
     1141 catcgacaac gatacgccga tattgaacca ccacaatccc actaagagca aagcaaaccg
     1201 aataacgaag ccaaacaaca cttcgccaac accggcgatc accagcgcct cacgcggaaa
     1261 attcactttc gtcagaatgg tgtgagattg ctgcaccatg cgcaagggca tgccaatggc
     1321 gtcgcagaaa acttgccaga gaatcaaacc ggcaaacaga aacagcgcat agggcgctgc
     1381 cgtttggccg acggtgaagt acccgctctg gcggagaaac acaaatacgg tagtcgagag
     1441 aaccggcggt aagaacgccc agatgtaacc gagcgcggta tgccggtaac gcgaactgag
     1501 gttacgcgcc agcaagcgcc aagccagttc gcgcgatgcc gcgatatcac agatcatatt
     1561 acggaacaga cgagcaggat cggtcagctg cgatgctggc gtgtattcgc agatcgtttg
     1621 tgaatttgcc cgttggttca tgcgattttt gtggatttta acgactgaaa atgtattcta
     1681 ctcgctgcat agtagttgat caacaaaatt ccgttcacga ctccacccgg tccacaaggt
     1741 ttgtgaacct cttggcgcgc caaaactcac gctggtaatc tccactaaat atcgactatg
     1801 agccggcccc cttcgctcaa aatccttttt ctgacatcaa gctatccgcg ccatgagcat
     1861 gacgtcgcgt cggtttttct tcactacttc gctagctcgt tggtggcgcg cggcaaccaa
     1921 gttcatgtgc tggcgccggc agaaggcaag gacggggttg cggccgacgg cgccattaca
     1981 gtgcaccgat tccaatactt tccgcaaagg tggcagaagc tcgcatacgg ctccggcatt
     2041 atgcccaatc tcagccgctc gcccctactt tggctgcaag tccctttcta tgtcttgtca
     2101 atgattttgg cgctggtgcg gctaatgcgt caggaaaaat tcgatgtgct ccacgcccat
     2161 tggattttgc cgcaaggctt aatcgccata gtcgctaaat caatccaacc gatcccactg
     2221 gtgaccacgg cgcatggcgc tgatgctttc gcgctcaacg gccgattgaa cggattcata
     2281 aaacggctgg taatttcaaa gagcgatgct tggaccgcta acacggaagc aacctgcgcc
     2341 gcggtcgaga ctaactactc caggacctcg gcccggatta ttccgatggg cgtcgaaatc
     2401 gagcggttct ccaatggcga tcgcagcgct ctgcgtggat tggtggatca aagtgagttt
     2461 ttgttgctct ttgtcggccg gctcgtcgaa aaaaaaggcg tcgacgattt gctccgggcc
     2521 ctcgcgttgt tgccagccgc tttcaggtca cgaaccaggc tttggattat cggcgaaggt
     2581 gatcttcgtt cgacgctgga aaaaactgcc gtggattgcg ccatcgatca acaggttcgt
     2641 ttttggggcg tcgtgcgcaa ccaggatttg ccagattatt atgcggctgc cgatcttttc
     2701 attgctccat caatcgaggc taagtcgggc gataccgaag ggcaaggcgt cgttttgtta
     2761 gaagcgtttg cggctcggtt gtgcgtcata gcgacccgct gcggcggcat cgacgctgtc
     2821 gtgcgcgacc agcacaccgg cattcttgtg ccacccaacg accccgaacg gatcgctgca
     2881 gccatcgaaa cattgattac ggacaccgcg ctacgaacca ggcttgccga caatgccttc
     2941 gccgaagtta gggaacgcta cgattggcga tacattgccg gagagttcga caagctctac
     3001 cggcaattgg tatcgtcagc tgcatttgtc acggattaag gcggtatgcg gtcatccgtc
     3061 atcatggcgg cgacccgcgt cttggcgttc catacgcagc agcgcaatct gctccgaaat
     3121 cagtcccaac atgaaaatga tcaccgacgt agtcaacgcg aacaccgaca tattggtaaa
     3181 acgattttgg gtaacatacg tgtagatgta attgccaatt ccaccaagga agaaaaacac
     3241 gctcactggc aaaaaaattc ggaacggcgc aaacaacgtc gcgatcttgg taatgatcaa
     3301 taggaaccgt atgccatccg tcaccaagct gatcttactc tggcccgcgc ggtaaagtgt
     3361 ctggatcggc acatacttga tggtcaatcc agagcgcagg aaagccaacg tcaatgtcgt
     3421 cgggtaagaa aacgtattgg gcagcaggtc gatgaaacgc agcgcatcac gccggcgcat
     3481 tgcgcgaaat ccagacgtca gatcctccac cttgaagtgc gtcacatacg acgcgagcag
     3541 attgtaaacc atgttggcca ggttgcggtg catgcgtagt ctggaacctt tggcgcgcgc
     3601 gccgacgacc atgtgaaaaa cgtccgcatc agccaacaat ttgggaatat cttcaggttt
     3661 gtgctgacca tcgccgtcca tcaaaacgat gaacttcccc ttggcggcgc gcataccgct
     3721 ctttatcgcc gcaccgttgc cgatgttgta aggatggcgc accacgttgg cccccgcggc
     3781 catggcgatc tcggcactgc catcgcaaga gccatcatcc acgacgagga tttcactgtc
     3841 gggcaattcg attgcgcgaa tccggcctat tagctttttc agattctccg cttcgttgaa
     3901 aatgggaatg ataactgaag tttcgactgg attagccatt caattgatag accgcgtgac
     3961 cgctctcacg gaagctcagc cgtagccgat tggcagtgaa ttcattccac aaacgcgctt
     4021 gattgggcgt aagattattg ttcaaaaacc tgctcagtag atcctcgcgg gtcatgaggt
     4081 gagtgatctg tttttgcctg aacgactgcg ccaactgctc ggtgtttttg gcgttgcgaa
     4141 tcgcccccaa aagaaatcct ggaagatctg cgccatcgtg aaaatagttt cgctggcaat
     4201 agtacgctct ccggccgaca aagagaagat aaattctcgc attcgcgggg gtttctcggt
     4261 tgataaagcg aaacgttgaa tattccggca gcgttcgtga gagataatca tcgcggctct
     4321 cctcgccgat gagataaggc caaggggccg cttccgcaat gtacttccac agatatacgc
     4381 catgccatgc ggcgaacacg aataacccgg cgaacaaaat gaccggccgc ttgatgcggg
     4441 tgtagatatt gaataccccg taaaccaaca ggatgatcag cggcgggacc attggcagca
     4501 cgtagcgcac gcgcaaatcg accaaaaata tcccatacaa aagcgtcagt aggaaaaacc
     4561 cagcgagcaa ttttttgtct tcacaccact taccccggaa agcccagggc aatagaacaa
     4621 tcagaatcgg cgtcagaacg ccgtcgaaga actgcggatt atcatcccga ccagaaaaaa
     4681 acagccgcag cggcagcgca acgatatgcc ataggtctt
//
LOCUS       DATLCH010000200         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4246243, whole genome shotgun sequence.
ACCESSION   DATLCH010000200 DATLCH010000000
VERSION     DATLCH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4246243"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1034)
                     /locus_tag="VMZ02_05975"
     CDS             complement(45..1034)
                     /locus_tag="VMZ02_05975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR71513.1"
                     /translation="MDLQELSQWMKERNLKGHWEHSEWSQTVKPCLWSGEEIMKALNW
                     SGELITTGEAGRRTIQMRNPGLSAGMTNTVHISVQLVKPGEVAAAHRHTAAAIRFVVK
                     GTPNAYTVVEGERFPMFEGDFITTPNWTWHDHFNNSTESVMWLDGLDVRLVTHFGAMI
                     QENFKKEQQPVEKPDNMSSKVFSHTRPSWVKNDFQAPPYRYPWEETNAALQALKESAG
                     DPHDGVLLEYTNPVDGGHTLPTCSCSIQLLRPKEKTKSHRHTCTTVYYAFRGQGVAKV
                     SANEFHWKQGDIFVVPSWLWHSHENSTSDDAILFSISDRPSTEALGLYREEGE"
     gene            complement(1254..1625)
                     /locus_tag="VMZ02_05980"
     CDS             complement(1254..1625)
                     /locus_tag="VMZ02_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71514.1"
                     /translation="MEFDQSLVSNTLEHRGCGIRAASYEVAPTRWLPEACLWVQLETG
                     ARKLWVRSFAHCFDADQLTFANKLEADNWALVAAKSIIDRAVEDRDAAADIPTGWRAN
                     RFSRIWRFTRRSLSPRLRSAR"
     gene            1830..>2971
                     /locus_tag="VMZ02_05985"
     CDS             1830..>2971
                     /locus_tag="VMZ02_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71515.1"
                     /translation="MPIMNYFCRWPGSIRRQWIPVVALMLLPGCVVERIGGAFTRQPE
                     ELEQSAATATKILIDQAFEGIDPAPPVDFHTHILAIGTSVNDAFVNPKMRRGLNLERL
                     KFLIYNSAADVKNFDNADQEYVNRLVRLARGSKRPGRFRILAFDKHYKRDGTVDLAKT
                     NMYVPNHYVVDLARQYPDIFSPVISLHPYRRDAIDELESWAKAGVKYVKWLPNAMGMD
                     PADRAIEPFYRKMKQHNMILLSHAGEEQAVQAEEDQELGNPLRLRFPLDMGIRVIVAH
                     AASLGNCADLDSGGAKKIFCFDLFLRLMDDAKYRGLVFGEISAMLQFNRMPRPFSTLL
                     KRQDLHPRLVNGSDYPLPAINALIRTRSMARDGFISSEERTALNEI"
BASE COUNT          666 a          843 c          784 g          678 t
ORIGIN      
        1 cttctttata tctatggcct agcacgattc acgaagatgc tatttcattc cccttcttcg
       61 cgatacaatc ccaacgcttc ggtcgagggc cgatcgctga ttgaaaacag aatcgcgtca
      121 tcgctcgttg agttctcgtg cgaatgccac aaccatgatg gcaccacgaa gatgtcaccc
      181 tgcttccaat gaaattcatt ggccgaaact ttagcgacgc cttggccgcg gaacgcatag
      241 tataccgtcg tgcaggtatg gcggtgcgat ttggtttttt cctttggccg cagcagttgg
      301 atcgagcacg agcaggtcgg cagcgtgtga cccccgtcga caggattggt atattccaac
      361 agaacgccgt cgtggggatc gccggcgctt tccttcagcg cttgcagcgc agcattggtt
      421 tcttcccacg gatagcggta cggcggcgct tgaaaatcat tcttcaccca gcttgggcgg
      481 gtgtgggaaa aaaccttgga cgacatgttg tcgggttttt ccaccggctg ctgttccttt
      541 ttaaaatttt cctggatcat cgcgccaaag tgcgtcacca gccggacgtc caatccgtcg
      601 agccacataa ccgactcggt ggagttgttg aaatgatcat gccatgtcca attgggcgtg
      661 gtgatgaagt cgccctcgaa catagggaaa cgctcgcctt ctacgaccgt gtaggcgttg
      721 ggcgtgcctt taacgacgaa gcggatcgcc gcggcagtgt ggcgatgcgc cgcggcgacc
      781 tcgccgggtt tcactaactg cactgagata tgcacggtat tggtcatgcc ggcagacagc
      841 ccgggattgc gcatttggat tgtgcgccgg ccggcttcgc cggtggtaat cagctcgccg
      901 gaccagttga gcgctttcat aatctcttca ccactccaca gacagggctt aacggtctgt
      961 gaccattccg agtgctccca gtggcccttg agatttcgtt ccttcatcca ttgactgagt
     1021 tcttgcagat ccataaacct ttccttccat acgaacacgt tgcgtcggag cttgttcaat
     1081 acaaacgggg catcgatctg tcaagtcacg atgatcgtca accagcgcgg cgatcccacg
     1141 caagcccaat tcaccgcgcc agcgaaatca gccgaggcgt tgcaacgcac aatggccggc
     1201 gtgctttgtc gcccggatga atatttttca gatcgctaga acgtgaattg gtattagcgc
     1261 gctgagcgca gtcgagggga aagtgaacgg cgcgtgaagc gccatatgcg tgaaaatcta
     1321 ttggcacgcc atcccgttgg gatatctgcg gcggcatccc gatcttcgac cgcacgatcg
     1381 ataatcgact ttgccgccac cagcgcccag ttatcggcct ctaacttgtt ggcgaacgtc
     1441 agttgatcgg cgtcgaagca atgggcaaag ctgcgcaccc aaagcttgcg tgcgccagtc
     1501 tccagttgca cccacagaca tgcttcgggc agccagcgtg tcggcgcaac ttcataggaa
     1561 gcggcacgaa tgccgcaacc gcggtgctct aaggtgttac tgacaaggct ttgatcaaac
     1621 tccacggcga atctccaaat aaattctaaa cagcgatggt gcgttgcagc gcaagctccg
     1681 taccagcgat tacaatgacc gtttagagca ttctagcgtc aatctttgca gccagcccgg
     1741 acaaaaatcg ttccacaggc tgatggcggt tcacttttct taccggcgtt tttcgttggt
     1801 ttgacagcct ctggcgatta tgaaaagtga tgcccatcat gaactatttc tgccgctggc
     1861 caggctctat tcggcgccaa tggatacctg tcgtagcgct catgctgttg cccggatgtg
     1921 ttgtcgagcg aattggcggc gctttcacac ggcaaccaga ggaacttgaa caaagcgccg
     1981 caactgcaac caaaattctc attgaccaag ctttcgaagg catcgacccg gccccacccg
     2041 tcgattttca cacacacatt ctcgccatcg gaactagcgt caatgatgcc tttgtcaacc
     2101 caaagatgcg tcgcggcctc aatctcgaac gtctcaagtt tcttatctat aacagcgctg
     2161 ccgacgttaa aaattttgac aacgcggatc aagagtacgt aaatcggctg gtgcgtctgg
     2221 cacgagggag taaacgtcct ggcagattcc gcatcctcgc cttcgataag cattacaaac
     2281 gagacggcac agtggattta gccaagacca acatgtatgt gccgaaccac tatgtcgtcg
     2341 acctggcgcg ccaatatccc gatatttttt cgccggtaat ttcgctccac ccctatcgcc
     2401 gggatgccat cgacgaactc gagagttggg ccaaagcggg agtcaaatat gtgaagtggc
     2461 tgcccaatgc catgggcatg gatcccgcgg atcgcgccat cgagccgttc taccggaaga
     2521 tgaaacaaca caatatgatt ctgctgtccc atgccggaga agaacaagcg gtgcaagccg
     2581 aggaggatca ggagctcggc aatccgctga gacttcgctt tcccctcgac atgggcattc
     2641 gcgtgatcgt cgcgcacgcg gcgagcctgg gtaattgcgc cgatctcgat agtggcggcg
     2701 ctaagaaaat tttctgcttc gatctttttc tgcgcttgat ggatgacgcg aaatatcgcg
     2761 gactagtctt cggcgaaatt tcggcgatgc tgcaattcaa ccgcatgccc cgcccgttct
     2821 cgacgctgct caaacgccaa gacctgcacc cgcgcctggt caacggcagc gactatccgc
     2881 tgccggcgat caacgcattg atccgaacaa gatcgatggc gagagatggc ttcatcagca
     2941 gcgaggaacg cacggcgctc aacgaaatct a
//
LOCUS       DATLCH010000201         4945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1289042, whole genome shotgun sequence.
ACCESSION   DATLCH010000201 DATLCH010000000
VERSION     DATLCH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289042"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..3126)
                     /locus_tag="VMZ02_05990"
     CDS             complement(256..3126)
                     /locus_tag="VMZ02_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HUR71516.1"
                     /translation="MSVSIGRDYFEIEAELKKRIEGDIRFDKYSRLLYSTDASIYQIE
                     PIGVVVPRHKRDVQAVLEVANRLGVSVLSRGGGTSLAGQAVGHSIVLDFSKYMQNVLE
                     VNEEELSCRVQPGVVQDELNAYVRALGLQFGPDTSTSNRATIGGMIGNNSSGAHSLTY
                     GKTLDHVLELTVLMADGNEVVLKDFAPAEVEHKSKADTLEGRAYREVYRLAQQHKPEI
                     LARYPKIMRRVSGYNLDEFVKPQPFNLARMIVGSEGTLAAIVEAKMRLVHKPKWTAMD
                     VIHFNDDIEALECAQAVLQTKPYAMESTDKMILNLARANIEQSRKLGFVQGTPDSLLM
                     VEYAGETEAQVRAQVERLEALRKREKIGYASTLAFEPAEVKAIWGVRKAGLGLLLGTK
                     GDKKPIAFVEDTAVSPEKLPEFIRRFREVVGRHDTVAGYYGHCSVGCMHIRPLINLKE
                     SSEKSKMVSIADAISDLVLEFNGSMSGEHGDGLARSHFNQKLFGAAIYEGFRQIKRAF
                     DPKNIMNPGKIVDAPAMTESLKISPQYKTWEPQTILDFSDQGGFARAVEMCSGMGECR
                     KKLDGTMCPSYMGTLDEEHSTRGRANALRNVIAGLVPKDEFTGKRLHDVMDLCLECKA
                     CKAECPSNVDMAKLKYEFLDHYHRANGLPLRNRIFGGIAALNHIGSTLAPISNWLAGS
                     SVNRWLMEFVAGIDRRRPLPQFASETFEHWFRAHKAEGDGSNGEVVLFHDTFNNYNTP
                     EVAIAATRFLEHSRYRVILIDKKCCGRPMISKGMLSQAKENSAWNIDRLAPYAEKGTP
                     IVGLEPSCLLTLRDEYPEFIRSPAAKKVALNSFLLEEFVMREVNAGRMTLRPVGRGKK
                     ILLHGHCHQKALVGTAPTVAMLRAAGYEVNEIDSGCCGMAGSFGFEKEHYDLSIKIGN
                     RRLVPAVESAGAEVEVVAPGISCRQQIEHLAGRGAKHPAELL"
     gene            complement(3178..4056)
                     /locus_tag="VMZ02_05995"
     CDS             complement(3178..4056)
                     /locus_tag="VMZ02_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HUR71517.1"
                     /translation="METGIFYAVIAGVFWGTSPVLVKRGLIHADVSAATLYQQATILA
                     TLIAAALVEGELFAGSIPFLAILIFACTGVVGAYLGRTLFVKTVDQIGASRAQSLNNS
                     SPLITVLLAALFLGEPLTLGVLIGVVLIISGVFFVTDTDAQDDRRVQRKILTLTSVMA
                     TICYGIVPVLKKFGTENGGTPVMGALVMHATGLLLLVTLGSLLKIEFKPERIAASSLL
                     CFIAAGFLYAIGSIMTLKALIHAPASVAAPVWSAQPIVSFFLVKTTLRGIEAVGWRDG
                     MAAVLVVLGVMVLRWG"
     gene            complement(4066..4749)
                     /locus_tag="VMZ02_06000"
     CDS             complement(4066..4749)
                     /locus_tag="VMZ02_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HUR71518.1"
                     /translation="MSAVAELNRRIVGCHKCPRLVRWREAAAKNPPRRYTGQNYWANP
                     LPSFGDAKARVLIVGLAPAAHGGNRTGRMFTGDRSGDWLYGALHEAGFASQPNSDHQG
                     DGLKLIDCYITAAVHCAPPGNKPNLIEFRRCRSYLVEELRLLKNVQVVIVLGKIAFDA
                     FLAAYRDSGVGLAQPRPKFGHGAAFQFPGTLRLICSYHPSQQNTFTGKLTRPMFRSVF
                     AAARAALST"
BASE COUNT         1099 a         1440 c         1334 g         1072 t
ORIGIN      
        1 gtttcgcaaa ggggtggaga gcggcgactt gaaacagtgc gataccttcg ccgcccgcgg
       61 tgtaatgtaa tcgctagctc gcggtcttcg ccccttcggt aattccggta atgtcggagc
      121 taacacgtct ggtccagcga aattttctac ttcgttgggc agccgcacta ctctgaccga
      181 caacacaaga tagtagcgat attactcgac gacttggagt tcaatgagaa ctaatccagg
      241 ctcgtttaac tgtgtctaaa gcagttcggc cggatgcttt gctccgcgtc cggccaagtg
      301 ctcaatctgc tgccggcagg aaatccccgg tgcgaccact tctacttcgg caccggcgct
      361 ctcaactgcc ggcaccaagc ggcgattgcc gattttgatc gacagatcgt agtgttcttt
      421 ttcgaaaccg aacgagcctg ccataccgca gcagcccgag tcgatttcat tgacttcata
      481 acctgcggcg cggagcatcg ccaccgttgg cgcagtgccc accagggctt tttgatgaca
      541 gtggccgtgc agcagaattt ttttgcctct gccgacaggt cgcagcgtca ttctgccggc
      601 attcacttcc cgcataacaa attcctcgag taaaaaacta ttgagcgcga ctttctttgc
      661 tgccgggctg cgaatgaact cgggatattc gtcgcgcaag gttaacaaac acgacggctc
      721 caaaccaacg atcggcgttc ccttttccgc gtaaggcgcg agccggtcga tattccacgc
      781 ggaattttcc ttggcttggc tgagcatccc tttggaaatc atcggccggc cgcagcactt
      841 cttgtcgatg agaataaccc gatagcgact gtgctcgagg aagcgcgtag cggcgatggc
      901 cacttcggga gtgttgtagt tgttgaatgt gtcgtggaac aaaaccactt caccgttgga
      961 gccgtctccc tccgccttgt gcgcgcgaaa ccaatgttcg aaagtctcgc tggcgaattg
     1021 tggcagcggg cggcgccgat cgatgccggc gacaaactcc atgagccagc ggttgaccga
     1081 tgaaccggcg agccaattgg aaatcggcgc aagagtcgaa ccgatgtggt tgagagcggc
     1141 gatccccccg aagatgcggt tgcgcagcgg caaaccgttc gcccggtgat agtggtcgag
     1201 gaactcgtat ttaagtttcg ccatgtcgac attggacggg cactcggcct tgcacgcttt
     1261 gcactcgagg cagagatcca tcacatcgtg caaacgcttg ccggtaaact catccttggg
     1321 caccaggcct gcgatgacgt tgcgcaaggc gttggcccgt ccgcgcgtgg agtgctcttc
     1381 gtcgagagtg cccatgtacg agggacacat cgtcccgtcg agcttcttgc ggcactcacc
     1441 catgccgctg cacatctcca ccgcccgcgc aaagccgcct tgatcgctaa aatccaagat
     1501 tgtctgcggc tcccaggttt tatattgcgg gctaattttc aacgattcgg tcatcgccgg
     1561 tgcatcgacg atcttaccgg gattcataat attttttgga tcgaaggccc gcttgatctg
     1621 gcggaagccc tcgtagattg ccgcgccgaa tagtttctga ttgaagtggc ttcgtgccaa
     1681 accgtcgcca tgttcgcccg acatagagcc gttgaactcg agcacgagat ccgagatggc
     1741 gtcggcgatc gagaccattt tgctcttttc gctgctctct ttgaggttaa tcagaggacg
     1801 aatatgcatg cagccgacgg aacagtggcc atagtatccg gccacggtat cgtgacgacc
     1861 aaccacctcg cgaaaccgcc tgataaactc aggcaatttt tccggcgaca ccgcagtatc
     1921 ttcgacaaaa gcaatcggtt tcttatcgcc cttggtccca agcaatagac ctaatccggc
     1981 tttgcgcacg ccccaaatcg ctttcacttc ggccggttca aacgcgagag tcgaggcata
     2041 accgatcttc tcgcgcttgc gcagcgcttc gagtctttcc acctgggcgc gcacctgcgc
     2101 ttcggtctca ccggcgtact cgaccatcaa aagagaatcg ggcgtgcctt gaacgaagcc
     2161 caacttgcgc gactgctcga tattagcgcg cgccaagttg agaatcattt tatcggtcga
     2221 ttccatggcg tagggtttgg tttgcaaaac agcttgggcg cattcgagcg cctcgatatc
     2281 gtcattgaaa tgaatcacat ccatcgccgt ccacttgggt ttatgcacca gccgcatctt
     2341 ggcttcgacg atggccgcca atgtcccttc cgaaccgacg atcatgcgcg ctaaattgaa
     2401 tggctgaggt ttaacgaact cgtcgagatt gtatcccgag acgcgccgca tgatttttgg
     2461 ataacgcgcc aggatctccg gcttatgctg ctgcgccaga cgataaactt ctcgataagc
     2521 ccgcccctca agggtgtctg ctttgctctt gtgttcgact tccgccggcg cgaagtcctt
     2581 gagtacgacc tcgttgccgt cggccatgag caccgtcagc tcgagaacat ggtcgagtgt
     2641 cttgccgtaa gtcagcgagt gcgcccccga cgagttgttg ccgatcattc cgccgatggt
     2701 ggcgcgattg gaggtcgagg tatccggccc gaattgcaag cctaaagcgc gcacgtaagc
     2761 gttcagctca tcttgcacca cccccggctg cacccggcac gacagttcct cttcgttgac
     2821 ttccaaaacg ttctgcatgt acttggaaaa atccaagaca atcgagtgac cgacggcttg
     2881 acccgcaagt gaggtgccgc cgccgcggga aagcacggag acgcccaggc gattggcaac
     2941 ttcgagcact gcttgcacgt cccttttatg gcgcggcaca accacgccaa tgggctcgat
     3001 ctgataaatc gatgcgtcgg tggaatacag cagccgcgaa tatttgtcga agcgaatatc
     3061 gccctcgatg cgcttcttga gctccgcttc gatctcaaaa taatcccggc cgatgctaac
     3121 gctcatgatc gcctcgcatt gagaactccg cattgcaaac tttgatcctt gaaaagttca
     3181 cccccacctc aataccatca ccccaagcac gaccagcacc gcagccatac cgtcgcgcca
     3241 acccaccgcc tcgatgcctc taagcgtagt cttcaccaag aaaaaactga cgatcggctg
     3301 ggcgctccag accggtgcgg ccaccgaagc gggcgcgtgg atcaaggctt tgagcgtcat
     3361 gatcgaaccg atggcgtata ggaaccccgc ggcaataaag cagaggagac tgctcgcggc
     3421 gattctttca ggtttgaatt cgatcttgag caagctcccc aaggtcacaa gcagcaatag
     3481 tccggtggcg tgcataacca aggcgcccat caccggcgtg ccgccgttct cggtaccgaa
     3541 tttcttcaac accggcacga tgccatagca gatcgtcgcc atcactgagg tgagagttag
     3601 aattttgcgc tggacgcgcc ggtcatcttg agcgtctgtg tcggttacga aaaacacgcc
     3661 ggaaattatc agcaccacgc cgatcaagac acctagcgtc agcggctcac ccaagaacag
     3721 cgccgccagc agtaccgtga tcagaggaga ggagttgttg agcgactgcg cccgcgacgc
     3781 gccgatttga tcgacggttt tgacgaacag tgtgcggccg agataagcac ccacgacacc
     3841 cgtacaagcg aaaattaaaa tcgccaaaaa tggaatactt ccggcaaaca gttcgccctc
     3901 gacaagcgcg gcggcgatca aagtggcgag aatcgttgcc tgttgataaa gcgtggctgc
     3961 gctgacgtcg gcatggatca aaccgcgctt gaccaatacc ggactggtac cccagaaaac
     4021 cccggcgatg acggcgtaga agatcccggt ttccacgttg gccgattaag tcgacagcgc
     4081 ggcgcgcgcc gctgcgaaaa cgcttcggaa catcggccgg gtaagcttgc cggtaaaagt
     4141 attctgctgg cttggatgat aagagcaaat caaacgcaaa gtgccgggga actgaaatgc
     4201 ggcgccgtgg ccgaatttcg ggcgcggctg agctaggcca acaccggaat ctcgataggc
     4261 cgcaagaaat gcatcgaagg caattttgcc gagcacaatc accacttgaa cattttttaa
     4321 aagccgcaat tcctcaacca agtacgaacg gcaccggcgg aactcgatga ggtttggctt
     4381 attccctggc ggcgcgcagt gcaccgctgc agtgatatag cagtcgatca gtttgagacc
     4441 gtcgccttga tgatcggaat tgggctgact ggcaaaaccg gcctcatgaa gagccccgta
     4501 aagccaatcg ccactgcgat cgccagtgaa catccgtccg gtgcgattgc cgccatgagc
     4561 cgccggtgcc aaaccgacga tcaggacgcg cgctttggca tcgccgaaac taggaagcgg
     4621 attggcccag tagttctgcc cggtatagcg tcgcggcggg tttttcgccg ccgcttcgcg
     4681 ccaacgcact agacgcggac acttgtggca gccaacgatg cgtcgattca gttcggcgac
     4741 agcgctcata gtcatagttt tgcaatcatt agttagattg aaatgttttt ccggacgcta
     4801 gcagtcggtg gtgtttttgt ctatcgcagc cgcctttagt tgttctatcc attaccggcc
     4861 gcatcaagat aacatgtctg tgctaacgcc atcgcgaaac gtagagtctg attgtcacac
     4921 agtgtccgcg ttgcgctccc aaaga
//
LOCUS       DATLCH010000202         4408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6232856, whole genome shotgun sequence.
ACCESSION   DATLCH010000202 DATLCH010000000
VERSION     DATLCH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6232856"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..974)
                     /locus_tag="VMZ02_06005"
     CDS             complement(54..974)
                     /locus_tag="VMZ02_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71519.1"
                     /translation="MRTEKVSFYSEGNKLSGVLYLPDGEQGKPYAGIVQGPGFLGLKD
                     AKHYIMMFEKLCAAGYACLCFDYRGWGDSEGSERGWVMPKWQAEDIRSALSFMETRPE
                     IDADRLATYGSGGTGGGNAVYVAAIDARVKCCVSYLGVSSGREWLHSMRREYEWIDYL
                     KSIEADRKVRAATGKGAIVSAREGGIMVQTPERVTTNIKKDVADKIPDAMPLACAEAI
                     LEYSPIDVVHKISPRGVLFIAVEGDAVTPEKQSFDLYEAAGEPKKLVLYKKTTHYGIY
                     NDYFDDVAANVVDWYNRHLKYEKVANTESE"
     gene            complement(1090..2085)
                     /locus_tag="VMZ02_06010"
     CDS             complement(1090..2085)
                     /locus_tag="VMZ02_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71520.1"
                     /translation="MATKLKFGLLLPHFCEYGSTELCIEGSKKAEAYGFDSVWVRDHL
                     VFEPHGMEGEDNTHIEGLLVLAAISTVCKKLTLGTGTVISHRHPIHLAQSMAGLSTMC
                     ENLIMGLGLGTFPHEFEAAGYKKVTLQDRANMAKINAQLCRRFWAGEKISYQDDYYDF
                     KDVELKPTPKKPIPIWYGGGTPASVRRAADYCDGWMPGRIPTATFNKMYKYLEEQCKI
                     AGRPMVTTGAIPITSIDKNRDVALSKVNTKGLINEGNAPSKKTWVRPKSGTFSTVEDI
                     EGLLMAGTPADVARDTKRYEEAGLNHIVFDLRFRYADWYQQIDYLGQEVLPAVRA"
     gene            complement(2249..3244)
                     /locus_tag="VMZ02_06015"
     CDS             complement(2249..3244)
                     /locus_tag="VMZ02_06015"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71521.1"
                     /translation="MSQIVRFFAVAFCFLSIPVLSFAQSKDLTRIRVSYSSIGAASLS
                     TWVAADAGIFNKHGLDVGLIYIGGGPRAMSTTIANETQITQGAGTGSILAKVGGADTV
                     MFATLLNTTPQSLMVHPDLRSPQELKGKKLGVTRFGALSDFGVRKYLQKINLDPEKDV
                     AILQLGGLPEILAAMQSGAIHGGALSSPALTKAKHLGFKELMDLGNLGIQYPGLSYMT
                     TEAYIKTHRPVVINFLKAIIESTHSLKRNKDFTVRVLKKYTKMDEPAVLDETYELFTQ
                     RYFRLVPTPSGEEVKTVLDQIKDPKARTTDYDSFIRTDLIREIEQSGFIKALTKG"
     gene            3506..>4408
                     /locus_tag="VMZ02_06020"
     CDS             3506..>4408
                     /locus_tag="VMZ02_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71522.1"
                     /translation="MAGPQKEFEVHPYVQFMTDKYSPYQNWIKSEGVPIIGGEFVQDV
                     RTEPLGYWQRKDCKGAICTFSDQMVADGYICEIDAGKSSKPQRQLYEEIITVAAGRGA
                     TSVWYDGTAKRTFEWERGSTFAIPLNSWHQHFNASGSEPCRYFALTSQPVAFELYRDP
                     EFIYNTNYQFKDRFDPGDAEFFSKEGKYFTEYYGGILHSNFIPDIRKINLVAREKRGK
                     GTRNMYIHMSGGSMLAHVSEFPVGRYKKAHRHGPGAHVFLLDSTGYTLMWQEGKEPQR
                     YDWHEGTVISPPAGAWHQHYNTGNR"
BASE COUNT          939 a         1195 c         1250 g         1024 t
ORIGIN      
        1 ggtcccgagt tgggcacgcg tacgccaaaa gaaattttgt tcatggtttg ctcctactcg
       61 ctctcggtgt ttgcgacctt ttcgtacttc aggtggcggt tgtaccagtc gaccacattg
      121 gcggcgacat cgtcgaagta gtcgttgtaa atgccgtagt gggtcgtctt cttataaagc
      181 accagcttct tgggttcgcc ggccgcttca taaagatcga aagactgttt ttccggcgtg
      241 acggcgtcgc cttcgacggc gatgaaaagc acgccgcgcg gcgaaatttt gtgcacgaca
      301 tcgatggggc tgtattccag gattgcttcg gcgcaggcga gcggcatggc atcgggaatc
      361 ttatcggcga catctttctt tatattggta gtgacgcgct cgggggtctg caccatgatg
      421 ccgccttcgc gagcgctgac gatggcgcct ttgccggtcg cagcgcgcac tttgcggtcg
      481 gcttcgatac ttttcaaata gtcgatccac tcgtactcgc ggcgcatgga gtggagccac
      541 tcccggccgc tgctgacgcc caagtagctg acgcagcact tgacccgtgc atcgatggcg
      601 gcgacgtaga cggcgttgcc gccgccggtg ccgcccgagc cgtaggtggc gagccggtcg
      661 gcatcgatct ccgggcgagt ctccatgaaa ctgagcgcgc tgcgaatgtc ttccgcttgc
      721 catttgggca tgacccagcc gcgctcgctg ccctcgctat cgccccagcc acggtaatcg
      781 aagcacagac aggcgtaacc ggccgcgcac agcttttcga acatcatgat gtagtgcttg
      841 gcgtctttga ggccgaggaa gccaggtccc tggacgatcc ccgcgtaggg tttgccttgt
      901 tcgccatccg gcaaatagag cacgccggag agcttgttgc cttcagaata gaaactaacc
      961 ttttcggtgc gcattagaga cctccaaaag tattcaaaaa gtcaaaaccg ttcaaatcgt
     1021 tcaaaacgtt gtcggatacg gaccggacgt tttgaactgt ttgaacggct tgaacgattt
     1081 gaaccttaat tatgctcgaa ccgccggcag aacttcttga cccagataat cgatctgctg
     1141 ataccagtcg gcgtagcgga agcgcaggtc gaacacgatg tgattcaagc cggcttcttc
     1201 gtagcgcttg gtgtcgcggg cgacatcggc gggggtgccg gccatcagta atccttcgat
     1261 gtcctcgacc gtggaaaacg tgccggactt cggtctcacc caggtctttt tactcggcgc
     1321 gttgccttcg ttgatcaagc ccttggtgtt gaccttgctg agcgcgacat cgcgattctt
     1381 atcgatgctg gtgatcggga tcgcaccggt ggtgaccatt ggccggccgg cgatcttgca
     1441 ctgctcttcg aggtatttgt acattttgtt gaacgtcgcc gtcggaatgc gcccgggcat
     1501 ccagccgtcg cagtaatccg ccgcccggcg cacggaagcc ggcgtgccgc cgccgtacca
     1561 gattggaatc ggttttttcg gcgtcggttt gagctcgacg tctttgaaat cgtagtaatc
     1621 gtcctggtag ctgatctttt cgccggccca gaagcggcgg cacagctggg cattgatctt
     1681 cgccatattg gcgcggtcct gaagcgtgac ttttttataa ccggcggctt cgaattcgtg
     1741 aggaaaagtg cccaaaccga gacccatgat gaggttctcg cacatggtgc tgaggccggc
     1801 catggactgt gccaaatgga tcggatggcg atgggagatg acagtgccgg tgccgagtgt
     1861 gagctttttg caaacggtgg agatcgccgc aagaaccagc aaaccctcga tgtgagtgtt
     1921 gtcttcacct tccatgccgt ggggctcgaa aaccaagtga tctctaaccc agaccgagtc
     1981 gaatccatag gcctcagcct tcttcgaccc ctcgatgcaa agttcggtgg aaccgtattc
     2041 gcaaaaatgc ggcagcaata atccaaattt cagctttgtc gccattcctc tatctccttg
     2101 tgatcaataa tgttttgatt gtgaagcaaa attcttatca cattttttgc ggcaaagtct
     2161 atgaggcgag cggggagcac gccggcggca aattaagaac gtttttcgat ctcgccgatc
     2221 cgaccggctg tgaaatccga ggctgcgttt agcctttggt caatgccttg atgaaaccgc
     2281 tttgctcgat ttcacgtatc agatcggtgc ggatgaagct gtcataatcg gtagtacgcg
     2341 ctttgggatc tttgatctgg tcaaggacgg ttttgacttc ctcaccggac ggcgtcggca
     2401 ccagacggaa ataacgctga gtaaacagct cgtaggtttc atcgagcacg gctggctcgt
     2461 ccatcttggt atattttttc agtacgcgga cggtgaaatc tttgttgcgc ttcaagctat
     2521 gggtcgactc gataatcgct ttgagaaaat tgatcacgac ggggcgatga gttttgatgt
     2581 acgcttcggt ggtcatgtag gaaaggccgg gatattgaat gccgagattg cctaaatcca
     2641 tcagctcttt gaagcctagg tgcttggcct tggtcaacgc cggcgaggac aacgcaccac
     2701 cgtgaatcgc gccactctgc atggccgcca aaatttccgg cagaccgccc aattgcagga
     2761 tggcgacgtc tttttccgga tcgagattga tcttctgcaa atatttgcgc acgccgaaat
     2821 cgctcagcgc gccgaagcgg gtgacgccaa gctttttacc ttttagctcc tgcggcgagc
     2881 gcaggtcggg gtggaccatc aagctttgcg gcgtagtatt gagcagcgtc gcgaacatga
     2941 cggtatcggc gccgccgacc ttcgccaaga tgctgccggt gccggcgcct tgagtaattt
     3001 gcgtttcgtt ggcgatggtc gtcgacatgg cgcgcggccc gccgccgatg tagatcaaac
     3061 cgacatcgag gccatgttta ttgaaaatcc cggcatccgc ggcgacccag gtcgacaacg
     3121 aggccgcgcc gatggacgaa tagctgacgc gaatgcgcgt gagatctttg ctctgggcaa
     3181 aactcagcac cgggatcgat aggaagcaaa acgcgacggc aaaaaaacgc acaatttggc
     3241 tcatggtaag ccctccacat gggtcgtata tcactcttgt cggctgtcaa gcaaagaaag
     3301 tttctatagc caagattcag catcagtggt gaaattggcg atcgcgcctt gggactatag
     3361 cgatggtgaa ctctgcgcgt agcacggtac gcaatccctc gtgtcctcgg taaatgttgt
     3421 ggtgtgatcc ccgccgtgat aattcgcacg attgatacag attcgccgcc gaagtaagtt
     3481 cgcctcaaat taatggaggt agtttatggc cggtccgcag aaagagtttg aagtccatcc
     3541 ttacgtccag ttcatgacgg acaagtacag cccttaccaa aattggatca aaagcgaggg
     3601 cgtgccgatc atcggtggtg agttcgttca ggacgtgcgc acggaaccat tgggctactg
     3661 gcagcgcaag gactgcaagg gggcgatctg cactttttcc gatcagatgg tcgccgacgg
     3721 ttatatctgc gagatcgatg cgggaaaaag ttccaagccg cagcgccaac tctacgaaga
     3781 aatcatcacg gtggcggccg ggcgcggcgc aacctcggta tggtacgacg gtacggccaa
     3841 gcgcacgttc gaatgggagc gtggcagcac gtttgcgatc ccattgaatt cttggcatca
     3901 gcatttcaat gccagcggca gcgaaccgtg ccggtacttc gcgttaacca gccagccggt
     3961 ggctttcgag ctctaccgcg atccggaatt tatctacaac accaactatc agttcaaaga
     4021 tcgcttcgat cccggcgacg ccgagttttt ttccaaagag ggaaagtatt ttaccgaata
     4081 ctatggcggg attttgcatt ccaattttat tcccgacatt cgcaagatca atctggttgc
     4141 gcgtgaaaag cgcggcaaag gcacgcgcaa tatgtacatc cacatgtccg gcggctccat
     4201 gctcgcgcat gtttccgaat tcccggttgg ccgttacaag aaagcgcacc gccacggtcc
     4261 cggcgcccat gtgttcctgc tcgattccac cggctacaca ctgatgtggc aggaaggcaa
     4321 agaaccgcag cgctacgact ggcacgaagg cacggtgatc tcgccgcccg ccggcgcctg
     4381 gcaccaacat tacaacaccg gcaaccgg
//
LOCUS       DATLCH010000203         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5017300, whole genome shotgun sequence.
ACCESSION   DATLCH010000203 DATLCH010000000
VERSION     DATLCH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5017300"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..1449)
                     /locus_tag="VMZ02_06025"
     CDS             complement(352..1449)
                     /locus_tag="VMZ02_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HUR71523.1"
                     /translation="MDRREFLKQGLTASAGLALAGMPKLAGWAHADDPTKWRTFEVTT
                     VIEVKDAVGGVRTWVPVPLTTATDYFKREPDTWTGNFKSARAVQYDKYGTGMIFAEWA
                     PTEKAPQLEVKSRFMTRDRAIDVNNKPVTPAKENPAVLNYFRQPSKLIRTDGIVGTTS
                     KSIVMGYKNDVDKAKAIYEWIVDNTFRDAKVKGCGIGDISTMLESGYLGGKCADLNAL
                     YVGLARAAGLPARDVYGVRVAASSEFKSLGRADDITGAQHCRAEVYLNGYGWLPIDPA
                     DVRKVVLEENLPGEQLPLTDPKVKRAREKLFGAWEMNWLPYNYAHDVKLPNSKLSDIG
                     FFMYPQLETANGRRDSLDPANFKYTIKSKEV"
     gene            complement(1550..2035)
                     /locus_tag="VMZ02_06030"
     CDS             complement(1550..2035)
                     /locus_tag="VMZ02_06030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1895..1897),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HUR71524.1"
                     /translation="MRMAGLEGKKAPAFTLDGNDGKKHSLEDYKGKTVVLFFYPKDST
                     PGUTKEACGFRDLNPSLKRKNVVVLGISRDSIESHNKFTEKYQLPYVLLSDPKTEVMR
                     KYNAFGKKMMYGKEVQGTIRSTVVIDPKGEVIKHWPSVKKAEEHPAEVAAYVGVGGKS
                     A"
     gene            complement(2087..2887)
                     /locus_tag="VMZ02_06035"
     CDS             complement(2087..2887)
                     /locus_tag="VMZ02_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUR71525.1"
                     /translation="MKVLKLASCMAQNADALCHFVAAILDTELGIRTEYVTGIPWQER
                     EQLFDAGEIDILWLCGLPYVDKAALADVGMELLAVPVPSHPRYKGRAVYYSDVVVHRN
                     SSFESFLDLRGAAFVYNEPRSHSGFNVMRAYLAEFGQRDGFFGRVVESGAHARSIEMI
                     LSGDVDTAAIDSTVLEWLQARDPELADKLRVVEIIGPSPIPPWVVSTTLPEVIRCRVR
                     QLLLNLHSASRGESALAHTQLAKFIPAHDRDYDPIRRMASAARAVLLA"
BASE COUNT          666 a          828 c          786 g          689 t
ORIGIN      
        1 aaaagcttga ccagaagtcg atcgaagacg ctgcggccga gtcgacgcgc aacattgaag
       61 tcatcgagga catcaacggc tcggcggagt accgcgccca tctcacgcag atctacgtcg
      121 cccgggcgat ccaggaagcg ctttcgcgct aggcgtcgac tctcagaact cccgtacaag
      181 attgtcacaa cggtgaaaac cggtgtccag gtggcgtgga gatgggactc cccgaggcga
      241 agatggatgc cggtttacac cggcatgacg gagttcggga gaacgcaacc aactataaaa
      301 gggagacccc gaatgctggg ggcctcgctt acgcaatttt tagaaatctt cctagacttc
      361 ttttgacttg atcgtgtact tgaagttggc cgggtccaaa ctatcgcggc ggccgttggc
      421 cgtttccagt tgcggataca tgaagaagcc gatgtccgaa agtttagaat tgggcagttt
      481 gacatcgtgg gcgtaattat acggcagcca gttcatctcc caggcaccga acaatttctc
      541 gcgagcccgc ttgaccttgg gatctgtcag cggcaactgt tcgcccggca agttctcttc
      601 gaggacgacc ttgcgaacgt cggccggatc tataggcaac cagccatagc cgttcagata
      661 gacttcggcg cggcaatgct gcgcgccggt aatatcatcg gcccgaccta aactcttgaa
      721 ttcagacgag gcggcgaccc gcacgccgta gacgtcgcgc gccggtaatc ctgccgcacg
      781 agcaagaccg acgtacaatg catttaagtc ggcgcatttc cctcctagat agccgctttc
      841 gagcatagtg gaaatatcgc cgatgccgca gcccttaacc ttggcatcgc ggaaggtgtt
      901 gtcgacgatc cattcgtaaa tcgctttggc cttgtcgaca tcgttcttgt atcccatcac
      961 gatactctta gatgtggtgc caacgattcc gtcggtgcga atcaatttgg agggttggcg
     1021 gaaataattg agcacggcgg gattttcttt tgccggcgtt acgggtttgt tgttcacatc
     1081 gatcgcccgg tcgcgcgtca taaagcggct cttcacttca agctgcggcg ctttttccgt
     1141 aggtgcccat tccgcaaaaa tcatcccggt gccgtacttg tcgtactgta ccgcgcgagc
     1201 ggatttgaag tttcccgtcc aggtatcggg ctcccgcttg aaataatcgg tggcggtggt
     1261 taggggcacc ggcacccaag tgcgcacacc gccaactgcg tccttcactt cgataacggt
     1321 cgttacttca aaggtgcgcc acttggttgg gtcatccgca tgtgcccagc cggccaattt
     1381 tggcatgcca gccaaggcca atcccgccga ggcggtcaac ccttgcttca aaaactcacg
     1441 gcgatccata gttcctccct gtcgagtcaa taggatttca cgatgcgcag ataataacct
     1501 ttagccctta attgtcaatt aatgttacag cacggaacat ctgagagcgt catgccgatt
     1561 ttccgcccac accaacatat gccgcaacct ccgccggatg ttcctcagcc tttttgacgg
     1621 acggccaatg cttaatcact tcgcctttgg gatcgataac aaccgttgag cgaatcgtac
     1681 cctggacttc tttgccgtac atcatctttt tgccgaaggc attatatttt ctcatgacct
     1741 cggttttcgg atcggacagc aacacgtagg gcagttgata cttctcagtg aacttattgt
     1801 gtgactcgat gctatccctg ctgatgccca agacgacaac atttttcctt ttgagtgacg
     1861 ggttcaaatc acggaaccca caggcttctt tagttcaacc gggagtactg tctttcggat
     1921 agaaaaacag cacaacggtt ttgcctttat aatcctccaa cgagtgcttt ttgccgtcgt
     1981 taccgtcaag ggtaaatgcc ggcgcttttt ttccttctaa acctgccatt ctcatcaacc
     2041 tcccatggat ttgacttgca acggtgcaaa ggttttagca cggactctag gcgagcaaaa
     2101 ccgctcgtgc agcgctcgcc atacggcgaa tcgggtcgta atcgcggtcg tgggcgggaa
     2161 taaatttggc gagctgagta tgcgccagcg cagactcgcc ccgtgaagct gagtgtagat
     2221 tcaacaatag ttggcgcacg cggcagcgaa tgacctcggg caacgtagtt gaaacaaccc
     2281 atggcgggat tgggctcggc ccgatgattt caacgacccg cagcttatcc gccagctcag
     2341 gatctcgggc ttgtagccac tccagtaccg tgctgtcaat cgcggcggta tcgacgtcgc
     2401 cggacaagat catttcaatc gaacgtgcat gagcgccaga ttcgacgact ctgccgaaaa
     2461 aaccatcgcg ctggccgaac tcggctaagt aggcgcgcat cacattgaaa cccgaatgag
     2521 aacgcggctc gttgtaaaca aaggcggcgc cgcgcagatc aaggaacgat tcaaatgaac
     2581 tgtttcggtg cacgacaacg tcggagtaat agaccgcgcg tcccttgtat cgtggatgcg
     2641 atggcaccgg cacggcgagc agttccatac ctacatcggc gagagccgct ttatcgacat
     2701 acggtaggcc gcacagccac aggatgtcga tttcgcctgc atcgaacaat tgttcccgct
     2761 cttgccacgg gatacccgtg acgtattcgg tgcggatgcc caactcggta tccaggatcg
     2821 cggcgacgaa gtggcaaagc gcgtcggcat tctgtgccat gcaggaggca agcttcaaga
     2881 ctttcatgca ggcgcgatct actcgacaat tttgacgtcg cgccagaatg caatatagtt
     2941 cttaatcgac ttggcggcgt ccttgggtt
//
LOCUS       DATLCH010000204         5789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2791547, whole genome shotgun sequence.
ACCESSION   DATLCH010000204 DATLCH010000000
VERSION     DATLCH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2791547"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VMZ02_06040"
     CDS             <1..355
                     /locus_tag="VMZ02_06040"
                     /inference="COORDINATES: protein motif:HMM:NF021001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2019 domain-containing protein"
                     /protein_id="HUR71526.1"
                     /translation="LNLASVTEIVDAYERGVKLNGLAIMNGDSEAANAAYDFVWRCSV
                     ELKKRGIDAQRTLLVLLKNGDPQVRLCAAKDALQFNPDLGQLELERIAKLDVIWSLHA
                     SLILEEWKRGRLRFP"
     gene            complement(388..549)
                     /locus_tag="VMZ02_06045"
     CDS             complement(388..549)
                     /locus_tag="VMZ02_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71527.1"
                     /translation="MTPIAVNDPDVFAEAKEKRIDVALITGEETENAGEGSGQSAAGD
                     CREVEETAG"
     gene            686..1666
                     /locus_tag="VMZ02_06050"
     CDS             686..1666
                     /locus_tag="VMZ02_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71528.1"
                     /translation="MQKIISAIVIVLSGLIVANACAATEPVRLKIIYSSFTGAYTPLW
                     LAVEEKLGRKYGLEFEAVYAGRARPHQLLLSGDAQYVVSTGTGVVSSYAVGTKDLVII
                     ASFVNATGSSIFSKPQIKTAKELRGKTLASGRPGAVSDILLHYILKRKIGLEPGRDVK
                     IVNVGDTPAILPALEKGVVDGAMLTTPSRLVAKRAGFRELVDFDDLGVQYPYVGISTL
                     KVNVKKSPDVTLRLVRALTDALQIFKTNRERSMTTMKKYLRGASDEMLDETYGYFSAR
                     MPRFPYPSVEAIKTALDMMVDQFPQAVSVDPQEVIDMSFVKQVESSAASK"
     gene            1676..2683
                     /locus_tag="VMZ02_06055"
     CDS             1676..2683
                     /locus_tag="VMZ02_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71529.1"
                     /translation="MTASFIAVLFFIVSSLTTLTAAAQAAESAPLERINFGFSAAGSV
                     ASAPLWMAQDSGAFKKYGLDVKLIFLAGGLAPVALMAGEVPFALMSAGVMVPAALRGA
                     DLIMVAALSNYLNQVLAVAPEFTDVKQLRGKRIAIQRLGDLTHIAAREALKHIGLPES
                     DLQMQQIGGGPARFTALQTGNVQAAILTPPYSTRARKLGYRIPVNLYDLKIPFIGTGV
                     VTTKQLVATRRPLVSNLLKAIAEGIRFYKRERDAPIKIAERYLPGAAREEIADALDHY
                     NRDLDERPYPRPEGFKIALDLTAQQLPLAKGVDAERFIDVSLLRELERSGFFAGLSAA
                     K"
     gene            complement(2825..4045)
                     /locus_tag="VMZ02_06060"
     CDS             complement(2825..4045)
                     /locus_tag="VMZ02_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71530.1"
                     /translation="MAEPAKLQLLTRKPGETGFQLHEIESSQAYLGYRPQRVPNELWE
                     IIAQPSSPDGRRNFILKNLQRDRYLLIDSHESFLWELLDGDHSLEEIARSFHHRFGAF
                     DYAMIRQLLAKLYQAGLLVERENLNLQQSLARSQANFWAMGFTKMAKCWRRLSFTLPN
                     ADRLCSRLYRCGGFVLFHPATVVLTVLLALPAGAAAIHLAPQAKQFAKMLSSKPWLST
                     AVMLASLLAVSMMHVTVHALACKAYNRRVREIGFFLLQGVLPTFYADVTDIFMSTRRA
                     RVIVDLAGPLVELFWGSVAFLAAYQASPGVAQAFLFGIGILFWEGVLINLYPFNFLEM
                     DGYNIVADLLAMPTLRQQALALAPRLHRRLLHPRTLTKAEWIQIAYLLLCIVSVLIYL
                     IAHLDFLGSLLPTW"
     gene            complement(4053..4568)
                     /locus_tag="VMZ02_06065"
     CDS             complement(4053..4568)
                     /locus_tag="VMZ02_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HUR71531.1"
                     /translation="MPPIPDILHKVVLFQDLQAESLATLARLLIAKKFAAGTVIFHEL
                     DDSDALYIIERGSVVVSKHVSGDVDMVLTRFTPGDFFGEMGLFDNAPRSATANVETDS
                     LLWQLDRGVFHDIFSHHPEMAAKICYRLVTIFIERLRVTNDQTRDAIRWGLEATGYAS
                     GNRSLFGRKST"
     gene            complement(4722..5660)
                     /locus_tag="VMZ02_06070"
     CDS             complement(4722..5660)
                     /locus_tag="VMZ02_06070"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HUR71532.1"
                     /translation="MIQWFNADFHAMGEVSKIKNIDHIDWVRCAPFIVMHLGCLGVFL
                     VGWSWTAVATACGLYFVRMFAITGVYHRYFSHRTYKTSRLTQFIFALLGASATQRGPL
                     WWAANHREHHRNSDTADDPHSPVQYGFFRSHAGWFMCRRYYATQYARIQDFARFPELV
                     WLNRFDKAVPLSLAVLLYIIGFSLHRAVPSLGVTGLQLFVWGFVVSTTVLFHATATIN
                     SLAHIFGNKRYDTGDESRNNFWLALITLGEGWHNNHHQHMGCVRQGFYWWEIDITYYI
                     LKALSVFGIIWDLKPVPVKAYDCNEQLPLKKMASAA"
BASE COUNT         1380 a         1501 c         1674 g         1234 t
ORIGIN      
        1 tctcaatttg gcaagtgtca cagaaattgt tgatgcatat gagcgtggag ttaaattaaa
       61 tgggctagcg ataatgaatg gggatagtga agcggcgaat gccgcatatg atttcgtgtg
      121 gagatgttca gtagaactta aaaaacgagg tatcgacgca caaaggacgt tgcttgtatt
      181 gctgaaaaat ggtgatccgc aggtgcgatt atgtgccgct aaagatgcgc tccagttcaa
      241 cccggatttg ggccagctag aactcgaacg aatcgcaaaa ttagatgtca tttggtctct
      301 gcacgcttcg ttgattctgg aagagtggaa gcgcggtaga ctgagatttc cgtagatctc
      361 gagcagatta tgcgcttgga tttctattca cccagcagtt tcttcaacct cgcgacaatc
      421 tccggcggcc gattgaccac ttccttcgcc agcgttttcg gtttcttcgc cggtgatcaa
      481 tgcgacatct attcttttct ctttcgcttc ggcgaaaacg tcggggtcgt taacggctat
      541 tggtgtcata tcagtacact tcggttcgac tacatcggtc cgaaaagatt tctcgcttcg
      601 ctcgaaatga caccgatgcc tgtttgacag tgcagcggat gagatagtat cgcatcaggc
      661 aagacagctt gaaacggata gaatcatgca aaagattatc agtgcgatcg tgatagttct
      721 cagcgggcta atcgttgcca acgcgtgcgc cgccactgaa ccggtgcgcc tcaagattat
      781 ttactcctct ttcacgggcg cctacacgcc gctctggttg gcggtggagg aaaaattggg
      841 ccgtaagtac ggattggaat ttgaagcggt gtacgccgga cgggcgcggc cacatcagct
      901 cttacttagc ggcgatgccc aatacgtcgt ctccaccggc acgggcgtag tttcgagcta
      961 tgccgtcggc acaaaagatt tggtgatcat cgcgtcgttc gtcaacgcca ccggctcgtc
     1021 gatcttttcc aagccgcaga tcaagaccgc caaggagctg cgcggcaaga cgttagcttc
     1081 tggccggccg ggcgcggtgt cggatatctt gctgcattac attctcaagc gaaagatcgg
     1141 gctggagcca ggacgcgatg ttaagatagt taatgtcggc gacactccag cgatcttgcc
     1201 tgctttggag aaaggcgtcg tcgacggcgc gatgctgacc acgccgtcgc ggctggtggc
     1261 gaagcgcgcc gggttccgcg aactggtcga tttcgacgat ctcggcgtgc agtatcccta
     1321 tgtcggtatt tcgacactga aagtgaatgt caaaaagagc cccgatgtca cgctgcggct
     1381 cgtgcgcgcg ctcacggacg cgctgcagat cttcaaaacc aaccgcgagc gcagtatgac
     1441 gacaatgaaa aaatatttgc gcggcgcaag cgacgagatg ctcgatgaaa cctacggtta
     1501 tttcagtgcg cgcatgccgc gctttcccta tccatccgtg gaagcgatca agaccgcgct
     1561 ggacatgatg gtcgatcaat ttccccaagc ggtgagcgta gatccccagg aagtgatcga
     1621 tatgtctttt gtaaaacaag tcgagagcag cgccgcatcg aaatagggga ccgtgatgac
     1681 tgcaagcttc atcgctgtgc tgtttttcat cgtttcaagt ctcacgacat taacagcggc
     1741 ggcgcaggcg gcggaatcgg cgcccctgga gcgcatcaac ttcggtttca gcgctgccgg
     1801 ttccgtggcc tcggcgccgt tgtggatggc ccaggacagc ggcgcattca aaaagtatgg
     1861 actcgacgtc aagttgatct ttcttgccgg cggcttggcg ccagtggcat tgatggcggg
     1921 tgaagtaccc ttcgcgctca tgtctgccgg agtcatggtg ccggcggcgc tccgcggcgc
     1981 ggatctgatc atggtcgcgg ccttgagcaa ttatctgaac caggtgctcg ccgtggcgcc
     2041 ggaattcacc gatgtgaaac agctcagagg gaaacggatc gcgatccagc gcttgggcga
     2101 cctgacgcat atcgcggcgc gcgaggcgct caaacatatc gggctccccg agtctgactt
     2161 gcagatgcaa cagatcggcg gcgggccggc gcgctttacc gcgctgcaga cgggtaacgt
     2221 ccaagcagcg atcttgacgc cgccgtatag cactcgagcg cgtaagctgg gctaccgcat
     2281 ccccgtcaat ctctatgatc taaaaattcc ctttatcggc accggagtgg tgacgacaaa
     2341 gcagttggtc gccacgcgca ggccgctagt gtcgaatctg ctcaaggcga tcgccgaagg
     2401 cattcgcttt tacaaacggg aacgcgacgc gccgatcaaa atcgctgaac gttacctgcc
     2461 gggagcggcg cgggaagaaa tcgccgacgc gctcgatcat tacaaccgcg atttggacga
     2521 gcggccttac ccgaggcctg aagggttcaa aatcgcactg gatttgacgg cgcaacaatt
     2581 gccgctcgcg aaaggcgtcg atgccgagcg gttcatcgac gtgtcgctgt tgcgtgagtt
     2641 ggagcgcagc ggcttcttcg cggggctcag cgctgcgaag tgatttaaca tgggtccaat
     2701 ttgcagtcga ttaaactagg taattacccc gaggtgccaa gatgcgaagg cgaaagtatt
     2761 cgttctgaac ttcgagctct tcgagcttgg gtggtatttc aacacaggtc ggtgaggtct
     2821 cgctttacca ggttggcagc aagctgccga ggaaatcgag atgagcgatg agataaataa
     2881 gcacggagac gatgcagagc aagagatagg cgatttgaat ccattccgcc ttggttagcg
     2941 tgcgcggatg cagcaagcgc cggtggagtc gcggcgccaa cgcgagcgct tgctggcgta
     3001 gggtcggcat tgccaacaga tcggcgacga tattatagcc gtccatttca agaaaattga
     3061 atggataaag attgatcagc acgccttccc agaacagtat accgatgccg aacaaaaacg
     3121 cttgagccac gccgggacta gcttgatacg cagctaaaaa agcgacgctt ccccaaaaca
     3181 gttcgactag aggtccggca agatcgacga tcacgcgcgc ccgccgcgtc gacatgaaga
     3241 tgtcggtgac gtcggcataa aacgtcggca agactccttg cagcagaaaa aatccaatct
     3301 cgcgcacgcg ccgattatag gctttacacg ccagcgcgtg cactgtgacg tgcatcatcg
     3361 acaccgccaa gagcgatgct aacatcactg ccgtgctgag ccagggcttg gatgaaagca
     3421 tcttagcgaa ctgcttggct tgcggcgcca agtgaatcgc cgcggctccc gccggcaacg
     3481 cgagtaggac ggttagcact actgtagcgg ggtgaaacaa gacaaacccg ccgcagcgat
     3541 ataatcgcga gcaaagccgg tcggcgttgg gcaatgtgaa agacaacctg cgccagcatt
     3601 tggccatttt tgtgaaaccc atggcccaaa aatttgcttg gctgcgcgcc aggctttgtt
     3661 gcaggttgag attttcccgt tcgacgagca gtccggcttg atagagcttc gccagcaact
     3721 ggcgaatcat cgcgtaatcg aaagcaccga agcggtgatg gaaagaacgg gcgatctctt
     3781 ccagggaatg atcgccgtct aaaagttccc agagaaacga ctcatgacta tcaattaata
     3841 aatagcggtc gcgctgaaga tttttcagga taaagttgcg tcggccatcc gggctggacg
     3901 gctgagcgat gatctcccag agttcgttcg gcacccgctg tgggcgatac ccgaggtaag
     3961 cttggctgct ctcgatctcg tggagttgaa aacctgtttc gcccggcttg cgagtgagca
     4021 gctggagttt cgccggctcg gccatgtcgc actcaggtgg attttcgtcc gaagagcgag
     4081 cggttgccgg aagcataccc ggtagcttcc aaaccccagc gaatagcatc gcgggtctga
     4141 tcgtttgtga cccgcagacg ttcgataaaa atcgttacca atcgatagca aatcttcgcg
     4201 gccatttcgg ggtgatgcga aaagatgtca tggaatacgc cgcgatcgag ttgccacagg
     4261 agcgagtcgg tttcgacgtt ggcggtggcc gagcgcggcg cgttgtcgaa tagtcccatc
     4321 tcgccaaaaa aatcgcccgg tgtgaagcgc gtcagcacca tatcgacatc gccactgaca
     4381 tgtttgctga ccaccaccga gccgcgttcg atgatataaa gcgcgtcgga atcgtccaat
     4441 tcgtggaaga ttaccgtgcc ggccgcaaac ttcttggcaa tcagcaatcg cgccagggtg
     4501 gctagggatt cagcttgcag atcctgaaac agaactacct tgtgcaaaat gtcaggtatg
     4561 ggcggcataa acgcgacgag ctattcggca aatgctagcg ggcgcgacga taaagcgcaa
     4621 gtaaacgcct ccgccgttaa accaacgatg tcgccgtcgc gtcagcgact cggccctgca
     4681 gattaaatag cggccgcttg tttgcgggaa ctttaggaag gctaggccgc cgacgccatt
     4741 ttcttgagcg gcagctgttc gttacagtcg tacgctttga ccggcacggg tttgaggtcc
     4801 cagatgatgc cgaacacgga caacgctttg agaatgtagt acgtaatgtc gatttcccac
     4861 cagtaaaacc cttgacggac gcagcccatg tgttggtgat gattgttgtg ccagccttcg
     4921 ccgagggtga tgagcgccag ccagaagtta tttctgctct catcgcccgt gtcatagcgt
     4981 ttattgccaa aaatatgcgc aagcgaattg atcgttgcgg tggcgtggaa cagcaccgtg
     5041 gtgctgacga caaaacccca aacgaagagc tgcaagccgg tcacgccgag cgatggcact
     5101 gctctatgta agctgaaacc gattatgtaa agcaagacgg ccaacgatag cggcacggcc
     5161 ttgtcgaagc gattgagcca aacgagttca gggaagcggg cgaaatcctg gatgcgcgca
     5221 tactgggtgg catagtaacg tcggcacatg aaccagccgg catgagagcg aaaaaaaccg
     5281 tattgaaccg gtgaatgggg atcatccgct gtatcggaat tgcggtgatg ttctctgtga
     5341 ttcgcggccc accaaagtgg tccgcgttgg gtcgcggaag cgcccaagag tgcgaagata
     5401 aactgtgtca aacgtgaggt tttgtaagta cgatgagaaa aataacgatg ataaacgccg
     5461 gtgatggcaa acatgcgcac gaaatagagg ccgcatgccg tggctacagc ggtccagctc
     5521 cagccgacca ggaacacgcc gagacacccc aaatgcatga cgatgaaagg ggcgcaacgc
     5581 acccaatcga tgtgatcgat attcttgatt ttgctgactt cccccatggc atggaaatca
     5641 gcattaaacc actgaatcat cgaggcggtg gcaacttgaa cgaatgagcg cttcattaac
     5701 aggagaacat accatgggga tggcaaggct tcaataaaaa ttgcgtgatt ttactgtgaa
     5761 aagcatgagc gctgcagcct cggtcatat
//
LOCUS       DATLCH010000205         7172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2793064, whole genome shotgun sequence.
ACCESSION   DATLCH010000205 DATLCH010000000
VERSION     DATLCH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2793064"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..822
                     /locus_tag="VMZ02_06075"
     CDS             229..822
                     /locus_tag="VMZ02_06075"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71533.1"
                     /translation="MRLSIWVLTFLISIPPAFAATSPLEAIKNTAGAVLSALRDPQLK
                     SNPKQRQERIESALRERFDFEEMAKRSLGPNWAKQSPTDQKQFVKQFTDLLISTYTDV
                     IEQYQGEEITYGAERRDGNQATVSTKIRDKKGTENSIDYRLMQQGDDWKVFDVVVADV
                     SLVNNYRSQFARLLAKSSFADLLQQLASKKFSSPGAS"
     gene            848..1516
                     /locus_tag="VMZ02_06080"
     CDS             848..1516
                     /locus_tag="VMZ02_06080"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUR71534.1"
                     /translation="MKKNWTALGITLVFVSGCGEIVTTSGGRGDPSRAESQLRTVPLD
                     RREAARLKDIMVPLLRVTNNHKSANNVRIGLISDPSINAANAGGGEFYITTGLLEKAN
                     DEQLRGVLAHEIAHDDLGHVARAQVLGAGLNIGMVLLEQILPGSSAVTPIAGTLIARG
                     YSRREEYAADSHGVELLERAGYSPQTLERTLEWILRQEKQGGGGGFLSSHPATEDRIA
                     ALRK"
     gene            complement(1556..2575)
                     /locus_tag="VMZ02_06085"
     CDS             complement(1556..2575)
                     /locus_tag="VMZ02_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HUR71535.1"
                     /translation="MSETNERWGIVLAGGEGNRVRGFLSALCGGRGIKQFCSILGGRS
                     LLQLTLDRVQRLIPRERILIIVDKNHREAREQLAEWPQANIIYQPANRETAPGILLPL
                     SYVSHRCPQATVAVFPSDHFIADESVFIRQVAKALDETDILPRSAVLLGVTPDRMEEG
                     YGWISSWKDGRGKDSQVVTGFWEKPTPLHAERLMREGALWNTFVFVARATTLWELTRL
                     TVPELYGAFTGLRIMLGTPRAQPFIEQLYGRLPSVNFSSQILTPSVSRLRVLPVPEIG
                     WSDWGSADRILETAMRMGRLEELAAKLHQGRIDDPTIMTLMSRYRNDFTPVRAYESGT
                     AAVLQ"
     gene            2846..3466
                     /locus_tag="VMZ02_06090"
     CDS             2846..3466
                     /locus_tag="VMZ02_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HUR71536.1"
                     /translation="MLTGALAGWSVAYLNATTTAPVDLDAVNAGWAKQITVKLAEHPQ
                     LGKNPLHVGVEVERELVVISGLVNSEAQHRQALDVSRAVNGSFYVRDQILVRVPREEF
                     TIDQAERQRAAARAAGERLGPALSDAWIHNDLSVRLRSDPMTAAQSALQVDVVDGRVF
                     LRGVVSTPAQKIAAAQAAAETQGVAEVYNELKTVLESGRPYRVSVG"
     gene            complement(3741..6557)
                     /locus_tag="VMZ02_06095"
     CDS             complement(3741..6557)
                     /locus_tag="VMZ02_06095"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR71537.1"
                     /translation="MKKLVRLDFLRRYFRNASLRTHLFALVVVSVLPFLIFAILVLNL
                     FTRHEKSFQAVGLRETTRALSSALDREFDATKTALEAVASLEDFDNEDLGQLYRKLRR
                     VLQSRPNWQTITLHDPAGNQVLNVLNPFGEPLPESEIERESFQALIRTKQPVPVNFHR
                     DAGSAARVGYRVPVLRGDNLKYVLSAELEPTVFGELLARQKLPDQTVGSIIDSRNVIV
                     ATSSRAELVGQPAGPLFNKLVPGQMDGWFEGPNRPGEFSYAALSRSPANGWSVALVVP
                     AAQLDAPLQRLFWSLGGAAVIFLVAGILLAVIVESRISASLQDLTAKAEGLGRGERVD
                     GTGNSRVKEVENLSQDIDRAVGLLEQRAHERDRAESALRELNDRLELRILERTVQLQS
                     ANDELIKEIRERQVAESALRSEHVYLSLLRSTERVIQEAPTAEAALTAALGQICRSFE
                     APFGYYAIPAMVKHGDVRTSFWCADGRTDQFFQLKSALQKASADGAGPAAEALKHSAV
                     HCTSLAAVKDSWARAGQAAGFVAVCAIPVAASGEPAGALAFFSEQPIELDQRLSTALR
                     QLETHLGRVIERHRADDALRLSEERFRTAFDEGPIGIGMVDLEMRYLRVNKALCEMLG
                     YTNRELLGQKFFAIVAAQDVDKLQGQLTGLVKGDAAGFRHETQMIAKSGEVLSCCVTA
                     ALIMGRHGTPGYILLLIENITESKRLEERLRETERLAAVGATSAMFAHEVGNPLNGIS
                     TTVQMIERDLSRSKNGVTPTVFSALEDIKSEITRLGALLHEFRYLARPQRLQTIPVRL
                     DQLVRDVLAWETFREKGISVKLDIPADLPSLSVDEEKLKQTIANLCDNAADAMPHGGI
                     LTIHAYQDRDEINLEIADTGIGIAPGVNVFELFTTNKPHGTGLGLAVVRQIVSAHGGR
                     VDYESQPGSGTTFTVTLPLNNHNG"
     gene            complement(6647..>7172)
                     /locus_tag="VMZ02_06100"
     CDS             complement(6647..>7172)
                     /locus_tag="VMZ02_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71538.1"
                     /translation="IFSSGNGLCRVDQEWFDHAIKGEIFGLPVLFCAPEEAIWSKAFI
                     MERERYDGADIAHLLLAAAQTMDWSRLLRRFGAEWRVLLSHLVLFGFIYPGKAGVIPA
                     WLLDELWQRLRAESTSNTNGPPLCRGTQLSRSQYLYDLENWGYIDARLYSPSGAMSAE
                     DVERWTHKALHYAK"
BASE COUNT         1606 a         2065 c         1978 g         1523 t
ORIGIN      
        1 ccaagttctc tagggtcagt gtcccatttc cgcaatgcgg ctcagaagct catcctggtt
       61 ttgacacaat cgtgggcgta ggctcccatg acgcttctaa gtaccggtgg cattcggttt
      121 gctcaatctt tgtcccaggt agggcgtact atgagtatgt tttcacaacc attgcgcgtg
      181 ctcacggcgc ttggttttaa atcgccgtcg tgagctcagg agatctgcat gcgtttatcg
      241 atctgggttc tcaccttctt aatttcgatt ccgccggcgt tcgccgccac ctcgcccttg
      301 gaggcaataa aaaataccgc tggggccgtg ctgtcggctc tgcgcgaccc gcaacttaaa
      361 tccaatccca aacaacgcca ggagcgcata gagagcgcgt tacgcgagcg ctttgatttt
      421 gaggagatgg cgaaacgctc cttggggccg aactgggcca aacagtcacc taccgatcag
      481 aaacagttcg tcaaacagtt caccgatctg cttatcagca cctataccga cgtcatcgaa
      541 caataccagg gcgaggaaat aacttatggt gcggaacggc gggacggtaa tcaggcgacg
      601 gtgagcacca aaatccgcga caagaagggc actgagaaca gcatcgatta ccgattaatg
      661 cagcagggtg acgattggaa agttttcgac gtggtggtgg cggatgtcag cctcgttaat
      721 aactatcgat cacaatttgc tcggcttctc gctaaatcca gttttgccga tctgctgcaa
      781 cagctcgcga gtaaaaaatt ctcaagccca ggggcatctt gagtgtcgcg aaaatcttga
      841 gcgttttatg aaaaaaaatt ggaccgccct ggggataacg ttggtttttg tgagcggctg
      901 cggcgagatc gttaccacat cgggcggccg aggtgacccg agccgagccg aatctcaact
      961 acgcaccgtg ccgttagacc gacgcgaagc ggcccggctg aaggacatta tggttccgct
     1021 cctccgcgtc actaacaatc acaagtcggc aaacaacgtg cgcatcggcc tgatttccga
     1081 cccgagtata aacgcggcca atgccggcgg cggcgaattt tatatcacca cgggtctgtt
     1141 ggagaaggcc aacgatgagc aactgcgcgg cgtgcttgct catgaaattg cccacgacga
     1201 cctcggccac gtagcccgcg cccaagtgct tggcgcgggt ttgaatatcg gcatggtgtt
     1261 gttggaacag atccttcccg gcagcagtgc agtgaccccc atcgccggta cgctgatcgc
     1321 gcgcggttac agccgtcgag aagaatacgc cgccgacagc cacggtgtgg agcttctcga
     1381 acgcgccggt tattcgccgc aaaccctgga acgaacactt gagtggattc tcagacaaga
     1441 aaaacagggt ggtggcggag gcttcctatc gtcgcacccc gcaaccgagg atcgtatcgc
     1501 cgcgttaaga aaatagcgcc tgaagagttt gtcttgcttg ctagttgtct gccttctact
     1561 ggagcacggc cgcggtgccg ctctcgtaag ctcgaactgg tgtgaaatcg tttcggtagc
     1621 gagacatgag cgtcatgatc gtcggatcgt cgattcgtcc ctggtgcagt ttcgcagcga
     1681 gttcctctaa tcgtcccatg cgcatggcgg tttcaagaat tctgtcggca ctgccccaat
     1741 cgctccagcc gatctcgggc accggtagta cccgcaagcg cgacacagac ggcgtcaaaa
     1801 tctgcgagct aaagttcacg ctcggtaaac gtccatacaa ttgttcgata aagggctgcg
     1861 cccgcggcgt ccccaacata atgcgtaggc cggtgaacgc gccgtaaagt tcagggacgg
     1921 tcagacgcgt caattcccag agcgtggtcg cgcgcgcgac aaacacaaag gtgttccata
     1981 aggcgccttc gcgcatcagc cgttcggcat ggagcggcgt cggtttctcc caaaaccccg
     2041 tcacaacctg gctatcttta cccctgccgt ccttccaact gctaatccaa ccgtaacctt
     2101 cctccatgcg atcgggggtt acccctaaaa gcaccgcgct acgcggcaag atatctgttt
     2161 cgtcgagggc tttggcgacc tgcctgataa agacactttc gtcggcaata aaatggtccg
     2221 atgggaacac cgcaacggtt gcttgcgggc agcggtggct cacgtatgac aggggcagca
     2281 agattcccgg tgcggtctcg cgattcgccg gctggtaaat aatatttgct tgcggccatt
     2341 cggccaattg ttcgcgcgcc tcacggtgat ttttatcgac gattatgaga atgcgctcgc
     2401 gggggattag ccgctgcacg cgatccaaag taagttgcag caacgagcgc ccgcccaaaa
     2461 tggagcagaa ttgtttgatg ccgcggccgc cgcataacgc cgacaaaaag ccgcgcacgc
     2521 ggttcccttc tccaccggcc aagacaatgc cccagcgttc gttggtctcc gacatagaca
     2581 gacaccccgc tcgcaaattt ccaagcgtta accgcaaaac cggagccata ttgcgcacga
     2641 aagccgcagc cgtaagtagt gtttatagct tgtgtcggga ttaactaaac ttgacacttg
     2701 ttggtccgct gtatcagtca tgcgaatcgc tcagcggact gtgtgccaga attgcgacac
     2761 agagagatcg tttgcgggaa aatattcggc acggagtttg ccgtgttagc ggtgaatggg
     2821 ccgcttttgc ttcacggttt tggcgatgct tacgggtgca ctggcgggct ggtcggtagc
     2881 ttacctaaat gccacgacca ccgcgcctgt cgacctcgac gccgtcaatg ccggttgggc
     2941 aaagcaaatc accgttaagc tcgccgagca tccgcagctg ggcaaaaacc cgcttcatgt
     3001 gggagttgaa gtcgaacgcg agctcgtagt gatttccggc ctagtaaact cggaagccca
     3061 gcaccgccag gcgttggacg tgtcacgcgc ggttaacggc agtttttatg tgcgcgacca
     3121 aattttagtg cgcgttccgc gcgaggaatt caccatcgac caagccgaac gccagcgggc
     3181 ggcggcgcgc gcggctggag aacgcttggg cccggcgttg agtgacgcat ggattcacaa
     3241 tgatctctcg gtccggctgc gcagcgatcc gatgaccgct gctcaatcag ccctgcaagt
     3301 cgacgttgtc gatggcaggg tttttctccg gggagtcgtc tcgacaccgg cgcaaaagat
     3361 cgcggcagct caggcagccg cggaaactca aggcgtcgcg gaggtttata acgagctgaa
     3421 aaccgttctt gagagcggcc ggccttaccg agtttcggtc ggctgatgca tctacgttgg
     3481 aaacgccaat gatcgcgtat gctgcgtccc gcgaacgctg ttccagcgta ctctcccggt
     3541 gaactggctt aagcggaaca aacctgtggc aatcaaatga aaaaacgccg caaaactaag
     3601 actaaaatca aggccaaaac gccgccgggg tgcgtccggt caggacgccg ggtcatgatc
     3661 gacagcaaga ctctgcaaaa ctcaaagaga cgcagcgact gatgctaaca ttgccatcgg
     3721 tgaaatcacc gcgatgagcg ctatccgttg tggttgttta gcggcagagt gacagtaaat
     3781 gtggtacccg accctggctg gctttcgtaa tcgacgcgcc cgccatgcgc cgagacgatt
     3841 tgtcgaacca ccgcgagtcc gagcccggtg ccgtgcggct tgtttgtggt gaataattca
     3901 aaaacattta ctcctggcgc gatgccaata cccgtgtcgg ctatctccag gtttatctcg
     3961 tcgcggtctt gataagcgtg gatggtaagt atgccgccat ggggcatggc atcggcagcg
     4021 ttatcgcaga gattagcgat cgtctgcttg agcttttctt cgtcgacact gagacttggt
     4081 aaatccgccg gaatgtcgag ttttaccgat attccttttt ctctgaaagt ctcccaggcc
     4141 aggacatcgc gaaccagttg gtccaagcgc actgggattg tttgcaaacg ttgcggccgc
     4201 gccaggtagc ggaactcatg aagcagagcg cccagtcggg tgatctcact ttttatgtcc
     4261 tcgagagccg aaaacaccgt gggcgtgaca ccgtttttcg accgggaaag atcgcgctcg
     4321 atcatctgca cagtcgtcga aatgccgttc aagggatttc ccacttcgtg agcaaacatc
     4381 gccgaagtcg cacccactgc cgcgagccgc tcggtctcgc gcagccgttc ctctagacgc
     4441 ttggattcag taatattttc aatgagaagt agtatatagc ccggggtccc atggcgaccc
     4501 atgatcaatg cagcggtcac gcaacaggaa agaacctcgc cgctcttggc aatcatctga
     4561 gtttcatgac ggaaacctgc ggcatcacct ttaaccagcc cggtgagttg accctgcagc
     4621 ttgtcgacat cctgcgccgc tacgatggcg aagaacttct gtcccaacag ctcacggttt
     4681 gtgtagccga gcatttcaca tagtgcctta ttgacgcgca aatagcgcat ctccaaatcc
     4741 accatgccaa taccgatggg tccctcatcg aatgcggttc gaaagcgttc ttcgcttagc
     4801 cgcaaggcgt cgtcggctcg gtgacgctcg atgacccgcc ccaaatgcgt ttccaactgc
     4861 cgcaacgccg tcgacaggcg ctggtctaat tcaatcggct gttctgaaaa gaatgccagc
     4921 gcacccgccg gctcgccact ggccgccacc ggaatcgcac agaccgcgac gaatcccgcg
     4981 gcctgcccgg cgcgtgccca cgagtccttt acggccgcca aactggtgca gtgtactgcg
     5041 ctatgcttta aagcttcggc tgcaggaccg gcgccgtcag cagacgcctt ctgtaatgct
     5101 gactttagtt ggaaaaattg gtccgtgcgt ccatcggcgc accaaaacga cgtgcgtacg
     5161 tcaccgtgtt taaccatcgc cgggatggca tagtaaccaa acggcgcttc gaaagagcgg
     5221 cagatctgtc caagcgccgc cgttaacgcg gcttccgccg tcggcgcctc ttggatcacc
     5281 cgttccgtgc tgcgcagcaa gctcaggtaa acatgttcag aacgcagagc cgattcggca
     5341 acctggcgtt cgcgaatttc ttttataagt tcgtcgtttg cactctgcaa ttgcacggta
     5401 cgttccaaga ttcgcaattc cagccgatcg ttcaattcgc gcagcgccga ttcggcacga
     5461 tcgcgttcgt gagcgcgctg ttcgagtaaa ccaacagcgc gatcgatgtc ctggctgaga
     5521 ttttccactt ctttgacccg ggaattgccg gtgccgtcga cgcgctcacc gcgccccagg
     5581 ccttcggctt tggctgtcag gtcttgcaaa gatgcggaga tgcgcgactc gactatgacc
     5641 gccagcaaaa tcccggcgac caaaaagatc acggccgctc cccccaacga ccaaaacaaa
     5701 cgctgcaatg gcgcatcgag ctgcgcggcc ggcaccacca gggccacgga ccaaccgttg
     5761 gccggtgatc ggctcaaagc cgcataggag aattctccgg ggcgattggg tccttcgaac
     5821 cagccatcca tttgcccggg aacgagttta ttgaataacg gtccagccgg ctgcccgaca
     5881 agctccgcgc gactactggt cgcgacgatg acattgcggc tgtcgataat gctaccgacg
     5941 gtttgatccg gcaacttttg ccgcgcgaga agctcgccaa acaccgtcgg ctccagttct
     6001 gcggaaagca catactttaa attgtcgccg cgtagtaccg gtacacgata gcccaccctg
     6061 gcggcgctcc ctgcgtcgcg atggaaatta accggcactg gctgcttggt ccgaatcaac
     6121 gcctgaaaac tttcccgttc aatctcggat tccggcagcg gctcgccgaa cggatttagc
     6181 acattcaaca cctggttgcc cgcgggatcg tgcagcgtga ttgtctgcca attcggccgg
     6241 ctctgtaaaa cgcgccgcaa tttacgatag agctggccta agtcttcgtt atcgaagtct
     6301 tccaacgagg cgactgcctc gagagccgtc ttggttgcat cgaattcgcg atcgagggca
     6361 gacgaaagcg ctcgagtggt ttcgcgtaga ccaaccgcct gaaaagattt ttcgtggcgc
     6421 gtgaaaagat taagtaccag aatagcgaag atcagaaacg gcagaaccga gacgacgacg
     6481 agggcgaaca aatgcgtgcg cagcgacgcg ttgcgaaaat atcggcgtaa aaagtcgagg
     6541 cggaccagtt ttttcatgcc agctaatgag cttaacccgt gatcgccaaa gttcaaaacg
     6601 tcttctttat gaagctcaca ggagatctgg cgcgccgcag gtatgattat ttggcataat
     6661 ggagcgcttt gtgtgtccag cgttcaacgt cttcggcgct catcgcacct gacggtgaat
     6721 ataagcgggc gtcgatatag ccccagttct ccaaatcgta gagatattgg ctgcgcgata
     6781 attgggtacc gcggcataac ggcgggccat tcgtattcga cgtcgattcg gcacgcagcc
     6841 tttgccaaag ctcgtcaagc aaccacgccg gtatcacacc ggctttcccg ggatagatga
     6901 agccgaaaag caccaaatgg ctcaatagca ctcgccactc tgcgccaaag cggcgtagca
     6961 gccgcgacca gtccattgtt tgtgctgctg cgagcagcaa atgcgcgatg tcggcgccat
     7021 cgtaacgctc acgttccatg ataaaagcct tggaccagat cgcctcttcg ggagcgcaaa
     7081 aaagcaccgg aagaccgaaa atctcaccct tgatagcgtg atcgaaccac tcctggtcca
     7141 cgcggcatag gccgtttccg gagctgaaga tg
//
LOCUS       DATLCH010000206         7407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6989267, whole genome shotgun sequence.
ACCESSION   DATLCH010000206 DATLCH010000000
VERSION     DATLCH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6989267"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VMZ02_06105"
     CDS             <1..471
                     /locus_tag="VMZ02_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUR71539.1"
                     /translation="MSKLRKILAPTDYSDLSIAGLRHAFESAYEAGAELIVLHVVEMG
                     DDWFSKSKNFGPVRTLMAEQKEFLDKFLRAKFGAFMNLVEVSQRVELGTAYVNIAEMA
                     EREAVDVVMMSTHGRTGLDHLLLGSITEKVIARAHCPVLAIPALYRHDPVFQAA"
     gene            875..2248
                     /locus_tag="VMZ02_06110"
     CDS             875..2248
                     /locus_tag="VMZ02_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HUR71540.1"
                     /translation="MESYSESIAKFAAGLKLADVPSEVVEKAKLVFLDTLGIALASST
                     MDFGTMVINVAKKLGGIADSLIIGTSSRVASANAVIANGTLAHGLDYDDTLEEAIVHT
                     GSCAWMTAFAVAEEKLAHGRALLEAAIVGTEVLCKIGLVAPGKFHARGFHPTAICSTF
                     GAAAAAGRLYDLSHAQWVDALGLCGSQSSGIIEYLADGTWTKRLHPGWSSHGGVVATL
                     LAKEGFRGPAKVFEGTNGFFPSFGGKNDYRYEKIAELGKIWEIPKLTFKSYPCGSISH
                     PYMDCALKIKQKYSPAPARIKEIVCRTAQGPVHRLWEPLADKQTPSSSYGAKFSLPYS
                     VAVMLVHGRAGLKEFTDEAIRDTEVLRLAAKVRYELDPTIDYPRHFSGHVKVFLDDGT
                     VLEENQPHPRGGLEDPLPPADIEAKFRANACLALPPERVEKIQAAVQHLEQLGSTREL
                     SDLLIQA"
     gene            2274..2945
                     /locus_tag="VMZ02_06115"
     CDS             2274..2945
                     /locus_tag="VMZ02_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HUR71541.1"
                     /translation="MKRVPIAVTKKFSILAKYILLLLFSVSCGGERFDHVRNLASPNE
                     VIVCLGDSLTEGVGADSGQDYPSVLSSELAFPVVNHGRRGDTTAQALTRLPEILEANP
                     RLVVVFLGGNDFLRQVPRSETKKNLAELVRRLQAHGAMVVIAGMGLGIFTDEFSPIYE
                     ATATQFGAYYIPQVMQGILNEPRLKSDQIHPNAAGYRLLGQRIAEKLKPLLREADRVT
                     GRHGF"
     gene            3305..4036
                     /locus_tag="VMZ02_06120"
     CDS             3305..4036
                     /locus_tag="VMZ02_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71542.1"
                     /translation="MSETKKLSGKVALITGGSRGIGRAIAAAYAEQGAQVFICGRNNN
                     DVRRTIDELRLGGAAIEGASGDIADPEDVRRIVHETVARFGTIDVLVNNASLLGPRVT
                     IAEYDFAAWREVLATNLDGLFLVTHEVLPIMLARHSGAIINLTSGVGRVGKAKWGAYA
                     VSKAALEGFTQVLADEVSAEGIRVNSVNPAATRTEMRAQAYPAEDPTTLPTPESIMPV
                     FVYLASDESRAVSGQALNAREWLGK"
     gene            4195..4332
                     /locus_tag="VMZ02_06125"
     CDS             4195..4332
                     /locus_tag="VMZ02_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71543.1"
                     /translation="MTNVEHQLNAYESRVRNLERRLQLIEAGVGVENASAVAEISVRV
                     Y"
     gene            4396..5373
                     /locus_tag="VMZ02_06130"
     CDS             4396..5373
                     /locus_tag="VMZ02_06130"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71544.1"
                     /translation="MIKIFLLLCSFLVGSVQAQDLKKIKIGYPAISYNQIHIWIAKDA
                     GIYRRHGLDAEVIFFRGGQMATQALVAGDPPIVNIGTVVPAGLQGHDIVLIASSESAY
                     SYSVVAKPAIARVEQLKGKRLGVSGFGSASHNAALILLKKFNLEAGKDVALVVAGPTM
                     ERLGAVDAGRIDATILTPSELPRARRQGLIEVFDMLELGIEVQGNGFATSRGFIRSQR
                     ETAVSALRSYVEAIYFIGKNRDESKRIAGKYMRTNEQDVLDATYDWFVKRVAKRPYPT
                     LKGIQYILDEMAPKMPQAKSAKPEQFVDLSLLQQLEKEGFFAEMNKRYQ"
     gene            5418..5546
                     /locus_tag="VMZ02_06135"
     CDS             5418..5546
                     /locus_tag="VMZ02_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71545.1"
                     /translation="MNLQIGQKFPDIELPDHEDQPAKLSELVGKFPFILTFYRGYW"
     gene            5559..5921
                     /locus_tag="VMZ02_06140"
     CDS             5559..5921
                     /locus_tag="VMZ02_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HUR71546.1"
                     /translation="MQLRHYVELQKELAVNYCHLAVCSVDSPDVNDAFRTGLGAQFPF
                     LSDHERKVVKLLDMTETSKSKGVTAMPHTFSLMPDLTIHNIYNGYWYLGRPTLDELRA
                     DLRAMLRKVRPDFAGPVG"
     gene            5990..6244
                     /locus_tag="VMZ02_06145"
     CDS             5990..6244
                     /locus_tag="VMZ02_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HUR71547.1"
                     /translation="MISPDEIKETLSKALPVALVETQDLTGGGDHWQVIIVSAAFEGK
                     GLLEQHRLVNEVLKEPLADQRIHALSLKTYSPEQWEKLGA"
     gene            6265..6588
                     /gene="grxD"
                     /locus_tag="VMZ02_06150"
     CDS             6265..6588
                     /gene="grxD"
                     /locus_tag="VMZ02_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HUR71548.1"
                     /translation="MAEDVISKIDEKIKNNKVMLYMKGTPDFPQCGFSAHTVEILKSY
                     GFPFASDDVLADAAVREGIKRYSNWPTIPQIYIDGKFVGGCDIVHELHERGELEPLLK
                     AAFSK"
     gene            6656..7054
                     /locus_tag="VMZ02_06155"
     CDS             6656..7054
                     /locus_tag="VMZ02_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR71549.1"
                     /translation="MKNRLAILGIVLSMALITSCSSVGRWGSHYDFETGMALFNQGRF
                     ADAIPYLEDATRENPRDAEAYLYLGRAYISESKWRKAIPPLRTAFRLSPRDTQQEIIN
                     LIIDAGFAAAINDFRLGDILDSPRRSKETL"
     gene            7089..>7407
                     /locus_tag="VMZ02_06160"
     CDS             7089..>7407
                     /locus_tag="VMZ02_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HUR71550.1"
                     /translation="MIRIKWCLVLLLLVSSVTPAAAQFDKLLKDLGGRLPSGSGGLND
                     AKIGSGLQEALKVGTENAVQQTGTVDGFLMNKAIKILMPKTLQSMEQPLRLVGYGPQI
                     DEFV"
BASE COUNT         1687 a         1921 c         2124 g         1675 t
ORIGIN      
        1 atgagcaagc taagaaagat tttggcaccg accgattatt ccgatttgtc gattgccggt
       61 ctacgccatg cgttcgagag tgcctacgaa gccggcgcgg agctgatcgt cctgcatgtg
      121 gtcgagatgg gcgatgactg gtttagcaaa agcaaaaact tcggccctgt gcgcacgttg
      181 atggcggaac agaaagagtt cctcgacaaa ttcttaagag caaagttcgg cgcattcatg
      241 aatctggtcg aagtgagtca gagggtcgaa ctgggtacag cctatgtcaa cattgccgag
      301 atggccgagc gtgaagccgt ggacgtcgtc atgatgtcaa cgcatggtcg cactggactc
      361 gatcatttat tgctgggcag cattaccgaa aaggtgattg cccgtgcaca ttgtccggtg
      421 ctagcgattc ctgcgcttta ccggcatgat cccgtgttcc aagcagcgtg agatgtggag
      481 taatcagtcg gcggctcaag ccattcgggg gatgcgccgc cgagtacatc ggaaaaattc
      541 gaactagtcg ggtcaatggt cgtgatcgcg tgtagaggcc cgtcgttcgc acgtgaaaac
      601 ttcctggctc aaccggtaaa atatccgtca ggcaactttg ctggagcgcc ttttaaaaac
      661 cagcacctcg ctagatgtcc ccgcctcggt cgcgactgat cggatagtca cttcaaattc
      721 acaacagata ttttgctgtc gacagctgtg gcgataggac tcgacctgtc cttgcgaatg
      781 gcatacaagc ttgcgcgcgt cactgaccac gtgtaacttc gctcgaaagt attatgcgtc
      841 aagtgctgta aagtcctgag gggagattca cgtcgtggag tcctattcgg aatcgatcgc
      901 taagttcgct gcgggcttga agcttgccga cgtgccgtcc gaagtcgttg agaaggcaaa
      961 attggttttt ctcgacaccc ttggtattgc cctggcatcg tcgacgatgg atttcggcac
     1021 catggtcatc aacgttgcca agaagctcgg cggtatcgcc gacagcctga tcatcggtac
     1081 ctcgagccgg gtggcgtccg ccaatgctgt gatcgccaac ggtactttag cgcatggttt
     1141 ggactacgac gatactttgg aagaggcgat cgttcacacc ggctcctgcg catggatgac
     1201 cgccttcgcg gtggccgagg aaaagctcgc gcacgggcga gcgcttctcg aagcggccat
     1261 cgtcggcacc gaggtgcttt gtaagattgg gctggtggcg ccgggaaagt ttcacgcccg
     1321 cggttttcat ccgacggcga tctgttcgac tttcggcgca gcggcggcgg cgggccggct
     1381 ctacgatttg agccatgctc aatgggtcga tgccttgggc ctatgcggca gccaaagctc
     1441 gggcatcatc gaatatttgg ccgatggcac ctggaccaag cgcttgcacc ctggatggtc
     1501 ttctcatggc ggcgtcgttg cgacgctgct ggccaaagaa ggctttcgcg ggccggcaaa
     1561 agttttcgaa gggaccaacg ggttttttcc atcgttcggc ggcaagaacg attaccgcta
     1621 cgaaaaaatc gccgagctcg gcaagatctg ggagattccc aagttgacgt ttaaatcgta
     1681 cccatgcggc tcgatctccc atccctatat ggattgcgct cttaaaatta aacagaaata
     1741 ttcgccggca ccggcgcgga tcaaagagat cgtctgtcgc accgcgcaag ggccggtgca
     1801 tcgtctgtgg gaaccattgg cggacaagca gacaccgagc agttcctacg gcgccaaatt
     1861 tagcttgccg tatagcgtcg ccgtcatgct tgttcacggc cgggctggat tgaaagaatt
     1921 taccgatgaa gcgattcgcg ataccgaggt tttacgcttg gccgccaaag ttcgttacga
     1981 actcgatcca accatcgatt atccacgcca tttttccggc cacgtcaaag tgtttctcga
     2041 cgacggcacg gtgcttgaag aaaatcagcc gcacccgcgc ggcggcttag aagacccgtt
     2101 gccgccggct gacatcgaag cgaaattccg tgccaacgct tgcctggcat tgccgccaga
     2161 gcgagtcgaa aaaattcaag ctgcggtgca gcacttggaa caactcggtt cgacgcggga
     2221 gctgagcgat ctgttgatcc aagcatgacg ctgtccctaa ctacttcccg gcggtgaaac
     2281 gcgtgccgat cgccgtcaca aaaaaattct ctattttggc caagtacatt ttgttgctgt
     2341 tgttttctgt cagttgtggc ggcgagagat tcgatcacgt gcgtaatctg gcgtcgccca
     2401 atgaagtgat tgtgtgtttg ggcgacagtt taaccgaagg agtcggtgcc gattcgggcc
     2461 aagattatcc atctgtcctg tcgagtgaac tggcttttcc tgtggtcaat cacggcaggc
     2521 gcggcgacac tacggcgcag gcgttgacgc gcttaccaga aatcctcgaa gccaatccgc
     2581 gcctagtggt cgtgttctta ggtggcaatg attttttgcg ccaagtgccg cgcagcgaga
     2641 ctaaaaagaa cttagcagag ctggtgcggc gtcttcaggc acatggagca atggtggtga
     2701 tcgccgggat gggactgggg atcttcactg atgaattcag tccgatatac gaagcgacgg
     2761 ctacgcagtt cggcgcctac tacatacccc aggtgatgca aggaattctc aacgaaccgc
     2821 ggttgaaaag cgatcagatt caccccaacg ccgccgggta ccgcttactc ggacagcgca
     2881 ttgccgaaaa gctcaagccg ttattgcgcg aggccgatcg agtaaccgga cgacacggct
     2941 tctagtcagg taagaaacag ccacgcccgt tcagtataat agctgtccag taatcacgcc
     3001 gctgattaaa ttggatcgct gaagccgaat agccagccca attgatcacg cttgaaatca
     3061 ccgaagacgc gaagagtttt ttaagcaata gacccagccg ggatgcaatc gatggcgaga
     3121 aaaggctgcc ggcgcgttct agagcaaatg acacactacg cgcacgagca ataatcccgc
     3181 cggcaccaag tataaatggc tgatgatcac gttattgcta aggcgagcaa taggaaataa
     3241 acactggtgc ggatccaaga tttcatcaac tctttgacta tgttatagcc gctatttgat
     3301 ggcaatgagc gaaactaaaa aactctccgg caaggttgcg ctaatcaccg gcggtagccg
     3361 cggcattggt cgcgcgatcg ccgcagccta tgcggagcag ggtgcgcaag tatttatctg
     3421 cgggcgcaac aataacgatg tccggcgtac tatcgacgaa cttcgactcg gcggcgccgc
     3481 catcgagggg gcgagcggcg atatcgctga ccctgaggat gtccggcgca tcgtgcacga
     3541 gaccgttgca cgcttcggca caatcgatgt gttggtgaat aacgccagct tgcttggacc
     3601 ccgcgttacg atagccgaat atgattttgc cgcctggcga gaagtcctag ccactaacct
     3661 ggatgggcta ttcctggtga cgcacgaggt gctgccgatc atgttagcgc gccactccgg
     3721 cgcgattatc aatctgacat cgggtgtcgg acgcgtggga aaagcgaaat ggggcgccta
     3781 tgccgtgtcg aaggcggcat tggagggctt cactcaggtg cttgccgacg aagtgagcgc
     3841 cgagggtatc cgggtaaact cggtcaatcc ggcggcgacc aggaccgaaa tgcgggctca
     3901 ggcctatcca gcggaagacc cgacgacgct gcccaccccg gaatcgatca tgccggtgtt
     3961 tgtttacctg gcgtcggacg agtccagggc agtttccggt caagccttga acgcccggga
     4021 gtggctcggc aaatgattga tctctccgga cgtttcgtgt aaagatcgcc cactatgccc
     4081 tgggtccaac gtaaaccaac tggtaaccgg atctattgga ttgcagtcgg agtcgcctcc
     4141 gtactgttcg gaattctgac gggttacgcg ttgtggggcc aaacggcttc tctagtgacc
     4201 aatgtcgaac accaactgaa cgcgtacgaa tcgcgagttc gcaacctcga aagacggctg
     4261 caactcatcg aagccggtgt cggggttgaa aatgccagcg cggtggcgga gatctccgtc
     4321 cgtgtttatt agttgaactg ctcgatttcc tgccagcatc gaggcgtcca ctcacatttt
     4381 tgggggcagt cattcatgat taaaattttc ctattacttt gtagttttct tgtcggcagt
     4441 gttcaggccc aagacctgaa gaagatcaag attggttatc cggcgatatc ctataatcaa
     4501 atacatattt ggatcgccaa ggacgcggga atctatcggc ggcatgggct cgatgccgaa
     4561 gtgattttct tccgcggcgg gcaaatggcg acccaggctt tggtcgccgg tgatccgccg
     4621 atcgtcaata tcggcaccgt cgtgccggcg ggtttacagg gtcatgacat tgttctgatc
     4681 gcgtcgtccg aatcggctta cagttattcc gtcgtcgcca agccggccat cgcgcgagtt
     4741 gaacagctga aaggcaagcg cttgggagtc agcggctttg gctccgcgtc tcacaatgcc
     4801 gcgttgattc tcttgaaaaa attcaatctc gaagcgggca aagacgttgc gctggtggta
     4861 gcaggtccaa ccatggagcg gctgggcgcc gtcgatgccg gtcgcatcga tgcgacgatt
     4921 ctcacgccgt ccgagctgcc gcgcgccagg cgccaaggtt tgatcgaagt cttcgacatg
     4981 ctcgaattgg gcatcgaagt tcaagggaac ggttttgcga cttcgcgcgg atttattcgc
     5041 agccagcgcg agacggcagt gagcgcgcta cgctcctatg tcgaggcaat ttattttatc
     5101 ggcaagaaca gggatgagtc caaacgcatt gccggcaagt acatgcgcac caacgagcag
     5161 gacgtgctcg atgccactta tgactggttt gttaagcggg tggccaagcg gccttacccg
     5221 actttgaaag ggattcaata tattctcgac gagatggcgc cgaaaatgcc gcaggccaaa
     5281 agcgccaagc cagaacagtt tgtcgacctg agcttgctgc agcagttgga aaaagaaggt
     5341 ttctttgccg agatgaacaa gcgttatcag tagctttgag gctgatgcgc attctaacct
     5401 aaaattaagg gggcgatatg aatctccaga ttggccagaa gtttcccgat atcgagttac
     5461 ccgaccatga agaccagccc gcgaagttgt ccgaattggt cggcaaattt ccctttatcc
     5521 tgacttttta ccgcggctac tggtgagcaa aatgccaggt gcagttgcgc cactatgtgg
     5581 aattgcagaa agagctggca gtgaattatt gtcacctagc cgtgtgcagt gtcgattcgc
     5641 cggatgttaa cgatgccttt cgcactggct tgggcgcgca gtttcccttc ttaagcgatc
     5701 acgagcgcaa agtcgtgaag ctgctcgaca tgacggagac ctcgaagagt aagggcgtga
     5761 ccgccatgcc gcatacattt tctttaatgc cggacttgac gattcacaac atctacaacg
     5821 gctactggta tttgggccgg ccgactctgg acgagctgcg cgccgatcta cgcgcaatgt
     5881 tacgcaaagt gcggccggat ttcgcgggtc ctgtgggata agcacagcac actggttaag
     5941 ttcatccggt ggagttgcaa gagcgcatgg ttgccggtaa gcttttcgta tgattagccc
     6001 tgacgagatc aaagaaacat tatccaaggc gctgccggta gcgcttgtcg agacccagga
     6061 tttgaccggc ggcggcgatc actggcaggt gattattgta tcggcggcgt ttgagggcaa
     6121 aggtctgctt gaacaacatc gactcgttaa cgaggtgctc aaggagccgt tggcggacca
     6181 gcggattcat gcgctgtcgc taaaaaccta cagtccggag caatgggaga agttgggcgc
     6241 ctaattgcaa aaggagagat gattatggca gaagacgtga ttagtaaaat cgatgagaag
     6301 atcaagaata ataaagttat gctttatatg aagggcacgc ccgattttcc tcagtgcggc
     6361 ttttcggcgc acaccgtgga gatactaaaa tcatacggct tccccttcgc gagcgacgac
     6421 gtgcttgccg acgcggcggt acgcgagggc atcaagcgct actcaaattg gccgacgatt
     6481 ccgcagattt acatcgatgg caagtttgtc ggtggctgtg acatcgtgca cgagctgcat
     6541 gagcgcggtg aactcgaacc gttgctcaaa gcagcttttt ccaagtaaat ttcacagccc
     6601 cgagtcttaa cgattcgggg ctttttgcgt ctaaattcca taggagggtg agtccatgaa
     6661 gaatcgtctc gccattctgg gcattgtact cagcatggct ctgatcacgt cgtgttcgtc
     6721 ggtaggccga tggggttcac actacgattt cgaaaccgga atggcgcttt ttaaccaagg
     6781 ccgcttcgcc gacgcgatcc cttatctgga agatgccacg cgcgagaatc ccagggacgc
     6841 ggaggcctat ctttatcttg gccgggctta tatcagcgag agcaagtggc gtaaggcaat
     6901 cccgccattg cgcaccgcct ttcggctgtc gccacgcgat acgcaacagg aaatcatcaa
     6961 cctgatcatc gacgccggat ttgcggcggc gattaatgat tttcgcttag gcgatattct
     7021 tgattcgccg cgccgttcca aagaaacttt gtaacagcgc ggcggctcgg ttttatagcg
     7081 aacattacat gatcagaatc aaatggtgct tggtgctatt gctcctcgtt tcgtccgtga
     7141 ctccagcggc agcccaattt gataagttat tgaaggatct cggcggcagg ctgccgagcg
     7201 gcagtggcgg cttgaatgac gcaaagatcg gttccgggct gcaagaggcg cttaaagtcg
     7261 gcaccgaaaa cgccgtccag cagaccggga ccgtcgacgg ttttttaatg aacaaagcca
     7321 tcaagattct catgcccaaa acgttgcagt ccatggaaca acctctacgg ctggttggct
     7381 atgggccgca gatcgacgag tttgtcg
//
LOCUS       DATLCH010000207         7419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4262316, whole genome shotgun sequence.
ACCESSION   DATLCH010000207 DATLCH010000000
VERSION     DATLCH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7419
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262316"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VMZ02_06165"
     CDS             <1..827
                     /locus_tag="VMZ02_06165"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HUR71551.1"
                     /translation="TQASFDEMVVAVDGLPVIFVNEKSTLRGSTHIARGASAVLTVKY
                     RSQGLDSWRYHFGDEVSQVRNFQLKMTTNFKDIDFPDNTLAPAQKRETKNGWELVWES
                     KNLLSGFQIGMIMPEKLQPGPLAGRISYFAPVSLLFFFFLMFIITALRGIELHPMNYF
                     FLACAFFAFHLLLAYLVDHISIHTAFVICSVVSLALVISYLRLVIGIRFAAVEAGLAQ
                     LMYLVLFSYAFFFKGFSGLAVTIGSILTLFVVMQLTGRIRWSERFASRAAVPSPGA"
     gene            869..1561
                     /locus_tag="VMZ02_06170"
     CDS             869..1561
                     /locus_tag="VMZ02_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR71552.1"
                     /translation="MAKQILVVDDDRHIREVVQFALEKAGFEVVEAGEGQQALALFKK
                     HAPALVVLDITMPEMDGLAVCREIRKSSDAPILFLSSRTDEVDRVVGLEIGGDDYVSK
                     PFSPRELVARVQAILKRTSLRQTAVDEIKELAHGAVRVDLYGHGAFWKDKRVALTATE
                     FAILQSLLREPQRVFSRDRLIDAAYQGQVTVTDRTIDSHMRNLRAKYAKAGCKDLIET
                     VHGVGYKLAPCR"
     gene            1552..3156
                     /locus_tag="VMZ02_06175"
     CDS             1552..3156
                     /locus_tag="VMZ02_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUR71553.1"
                     /translation="MPLTSKSRGRRFKLRTILLAVSLAVLILPLGSIYVFRFYEGELV
                     KQTESELIVQAALIAAVYKREVETLLKLDTRVADYGAKVALEQVKDDYFRPVKPQLSL
                     SNYRIRSRPADAAVAEQQADKIAQEVGARLMPVLFDAQRTSLAGVRLVDWQGIVIGGR
                     EDLGKSLAHISEVKQALQGRYGSAIRQRAVRRSQPPLASISRGTGVRVFVAYPILAGD
                     RLYGAVFLSRTPSSILEHLYGQKEKVILAAATVLLLVIALVVFTSYAIARPLHGLIDQ
                     TKRFAEGDKKALEPLNKPVTEEVALLSRSFSEMARNVEHRSEYIRNFAAHVSHEFKTP
                     LAAIQGAIELLDEHAEEMSTEKRSRFLRNIGQDTERLKRLVDRLLEMARADVMEPAAG
                     ECAVAPLVEQLRARYGGAQGLSLSLAGDTDVNARVPKEIFETVLSNLCDNSRQNGANH
                     VNITIRQLAGALSITIADNGAGVSPANAEKIFTPFFTTHREEGGTGLGLGIVRALLKA
                     YGGDIEFLPAEKGAAFTIKVPMAAHL"
     gene            complement(3187..3624)
                     /locus_tag="VMZ02_06180"
     CDS             complement(3187..3624)
                     /locus_tag="VMZ02_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HUR71554.1"
                     /translation="MIAVDTNILVYAHREDSPWHEGAYKIVVDLAESRALWSIPWPCM
                     HEFLAIVTHPRIYNPPTPLAAALDQVDSWIESPSLSLIRESEGYWPHLKSTLEQARIS
                     GPKVHDARIAAICLLHGVRELWTADRDFSRFPTLTTRNPLVTY"
     gene            complement(3621..3866)
                     /locus_tag="VMZ02_06185"
     CDS             complement(3621..3866)
                     /locus_tag="VMZ02_06185"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HUR71555.1"
                     /translation="MKTTVEISDNLLDEAKRLAARERTTVKALIEQGLRQIISTKKKP
                     GVFRLRKATFKGQGLQSGVSTATWEQIRDMAYEGHGG"
     gene            complement(3904..5658)
                     /locus_tag="VMZ02_06190"
     CDS             complement(3904..5658)
                     /locus_tag="VMZ02_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUR71556.1"
                     /translation="MLTRQYNRFRRAEYMRYNASHMRVLLVQPPVQDFYDTDVRLQPI
                     GLCYLKAAVSKHLPDIEVIVRDYHRGVGRKTVQIPKELRYLSDYYGAADKSPFSTFNR
                     YYHFGMPFDAIEEDISLLKPDVVGISSLFTAYFREALEVAARVKKVSQAMVVMGGSHA
                     SAAPESLLASPHVDYVIRSEGERPLVEFLRCLRDQQPVEAVANLAYKHRGEFVLNPIA
                     DNFPIEELPVPDVGDATPQSYTLAGKPLTFMITSRSCPHKCSFCSVHSTFGTNYRRRS
                     LEQVLEEIELRHRQGYRVIDFEDDNLTYYKQTFKQLCRRLIARFPNREMQFVAMNGIS
                     YLSLDDELLELMHQAGFSHLNLALVSSDKTVRETTKRPHTLEAYVKVVSKAHELGFKI
                     VSYQILGLPNESLDSMIQTLAFNARLPVLLGASPFYRIPNSPIARGLKLTEGDFVKAR
                     LTALAIETDHFTRDDIYTLFVTTRIVNYLKGLPGDGETNVFELMNQQGLNSRSQIGFD
                     LLKRLCETGRLHFATTHGLLENKKFRGELFARVFDEIGTIRCHNGRTIAVGEFGQQLA
                     GQLTRAARLDCRKYSEID"
     gene            5709..6776
                     /locus_tag="VMZ02_06195"
     CDS             5709..6776
                     /locus_tag="VMZ02_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HUR71557.1"
                     /translation="MKIEKIDSKKLEMPYTKPLVTATNNFTVARGLLVTVVTSGGVEG
                     YGYADLFPRAGETPETAAHVIETVLKAKALGKELEDLGRIRAEMDHALTGHPRAKSVL
                     EGAMYDALARSYHIPMSVMLGGKCRNEIKVIKMVSVGDPEAMAVEAKGLASEGLSVKL
                     KVSGRIDKDLPRVAAVRKAIGDDVFIKIDANEAYDAKTAIRLAKALADLGVEVFEQPV
                     PRDQFDALWEVKKHSPIKIEADQSCRNFADAQILVKNRMVDSINTGIPKVGSVGEVRR
                     IAELCEANGIRCSLSNTAGSVVGDAAALHLAVSTPGISPLCELGEFEVITGDPFFGLA
                     VTNGTLEVPEGEGLGVNLRGF"
     gene            7045..>7419
                     /locus_tag="VMZ02_06200"
     CDS             7045..>7419
                     /locus_tag="VMZ02_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71558.1"
                     /translation="MSSAFAAAMDKIVADYNDRLEWVRASKYDLTKESARVLIEQWSS
                     FTRHSRQCWAYVVGNCPHVEVRKFVVTENLYEEEALEGHSHFEILARMGIALGLTRDQ
                     IVNAKPLPTTVVAMRAWEALTKN"
BASE COUNT         1627 a         1941 c         2173 g         1678 t
ORIGIN      
        1 caacgcaggc gagtttcgat gaaatggttg tcgcggtcga tggtttaccg gtcatcttcg
       61 ttaatgaaaa atctaccttg cgcggcagca cccatatcgc ccgcggcgca agcgcagtat
      121 taacggtaaa ataccgatcc caaggcctgg atagctggcg ctaccatttt ggcgacgagg
      181 tctcgcaggt gcgtaacttc cagctcaaaa tgaccaccaa cttcaaagat atagattttc
      241 ccgacaatac tctggcaccg gcgcaaaaac gcgaaaccaa gaacggttgg gagctggttt
      301 gggagtcaaa aaacctgttg tcgggattcc aaatcggtat gatcatgccg gagaagctgc
      361 aacccggtcc gctggccgga agaatcagct actttgcccc ggtatcgttg ctgttttttt
      421 tctttttgat gttcatcatc acggctctgc gcggcatcga gctccatccg atgaactatt
      481 tctttctcgc ctgcgcgttt ttcgcttttc atctgctgct cgcttattta gtcgatcaca
      541 tttcaatcca cacggccttt gtcatctgtt ctgtcgtgtc gctggcgttg gtgatcagct
      601 atctgcgcct ggtcatcggt attcgcttcg cggcagtaga ggccgggttg gcacagctta
      661 tgtatctagt gctgttttcc tacgcgtttt tcttcaaggg cttcagcggc ctggctgtca
      721 ccatcggctc gatccttacc ttgttcgtcg tcatgcaact caccgggcgc atccgttggt
      781 cggagcggtt tgccagccgc gcagcagtgc cgtcgcccgg cgcgtgattg gtttttttcc
      841 cgtgagccat tatcgtaggc gctaatctat ggcaaagcag atccttgtcg tcgatgacga
      901 ccggcatatt cgcgaggtgg tgcagttcgc cttggagaaa gccggcttcg aggtggtcga
      961 agccggcgaa ggccagcagg cattggcact gttcaagaag cacgccccgg cgctggtcgt
     1021 actggatatc accatgccgg aaatggatgg cttggcggtg tgccgggaga ttcgcaagag
     1081 ctctgatgcg ccgattttat ttctatcgtc gcgcaccgat gaggtcgatc gcgtggtcgg
     1141 cttggaaatc ggcggcgatg actatgtcag caagccgttc agcccgcgcg agctggtggc
     1201 gcgcgtgcaa gccatcctca aacggacgag cctccggcaa accgccgtcg acgaaatcaa
     1261 agagctcgcg cacggcgcag tgcgcgtcga tctttacggc catggggctt tttggaagga
     1321 taagcgcgta gcgctgaccg ccaccgaatt cgccatactc caaagcctgt tgcgcgaacc
     1381 gcagcgggtc ttttcacgcg accgcttgat cgatgcggct taccagggac aggtgacggt
     1441 aaccgaccgg accatcgaca gccatatgcg taatctgcgc gcgaaatatg ccaaagccgg
     1501 ttgtaaggat ctcatcgaga ctgtccatgg cgtcggctac aaattggctc catgccgtta
     1561 accagtaaaa gcagagggcg acgtttcaag ctgcgcacga ttctcctggc ggtgagcttg
     1621 gcggttttga tcctgccttt gggcagcatt tatgtgtttc ggttttacga gggagaactc
     1681 gtcaaacaga ccgagagcga gctaatcgtc caggcggcgt tgatcgcagc ggtctacaag
     1741 cgcgaagtcg aaactttact caaactcgac acccgcgtcg cagactacgg cgccaaagtt
     1801 gcgctggagc aggttaaaga cgattatttt cgaccggtga aaccacagct aagtttgtcc
     1861 aattatcgga tccggtcgcg cccggcggat gccgcggtgg ccgaacagca ggccgacaag
     1921 atcgctcaag aagtcggtgc tcgactgatg ccggttttgt tcgatgccca gcgcaccagc
     1981 ttggcgggag tcagacttgt cgactggcag ggtatcgtta tcggcgggcg tgaagatctc
     2041 ggcaaatcgt tggctcacat cagtgaagta aagcaggcgt tgcagggccg ctatggcagc
     2101 gcgattcgcc agcgcgcggt tcgccggtcg cagccgccat tggcgtcgat cagccgtggc
     2161 accggcgtgc gcgtgttcgt cgcctatccg atattggccg gtgatcgatt gtacggtgcg
     2221 gtgtttttgt cgcgcacgcc gagcagcatt ctcgagcatc tttacggcca aaaagaaaaa
     2281 gtcatcctgg cggcggcgac cgttctgctg ctggtcatcg ctctggttgt cttcacgtct
     2341 tacgcgatcg ccaggccgtt gcatggattg atcgaccaga ccaaacgctt tgccgagggc
     2401 gataagaaag cactggagcc gctgaacaag ccggtgaccg aggaagtggc actgttgtcg
     2461 cgaagttttt ctgaaatggc gcgtaacgtg gagcatcgct ccgaatatat ccgcaatttc
     2521 gccgcccatg tttcccatga attcaaaacg ccgttggcgg cgatccaagg agccatcgag
     2581 ttgctcgacg aacatgctga ggagatgagc acagaaaaac gcagccggtt tctgcgcaat
     2641 atcggccaag ataccgagcg actgaaacgc ttggtcgatc gcttgctcga gatggcgcgc
     2701 gccgatgtca tggaaccggc cgccggtgaa tgcgctgtcg cgccgctggt cgagcagctt
     2761 agggcgcgct acggaggcgc tcagggtttg tcgctctccc tggccggtga cacggacgtt
     2821 aacgcgagag taccgaaaga aattttcgaa accgtcttga gtaacttatg cgacaatagc
     2881 cggcaaaacg gtgcgaacca tgtgaatatt acgatccgtc agcttgcagg cgcactcagc
     2941 atcactatcg ctgacaacgg cgcgggagtt tcgccggcta acgcagaaaa aatctttaca
     3001 ccattcttta ccacccaccg ggaagaggga ggtacgggtc tgggattggg gatcgtacgc
     3061 gcgctgctaa aagcctatgg cggcgatatc gaatttctcc cggcggaaaa aggtgcggcg
     3121 tttacgataa aggttccgat ggcggcgcat ctataacttt caagcagtaa ccaactttgt
     3181 gttgcgtcag taagtgacga gtggattgcg agtggtcagt gtcggaaatc ggctgaagtc
     3241 acgatccgct gtccacagtt ctctcacgcc atgaagtaag cagatcgccg cgatgcgcgc
     3301 atcatgcacc ttagggcccg agattcgggc ttgttcgagg gtgcttttga ggtgcggcca
     3361 atagccttcg gattcccgaa tcaaacttag actcggcgac tcgatccaag aatctacttg
     3421 atccaatgca gcggcaagag gtgtcggcgg attgtaaata cgcgggtgag taacgattgc
     3481 gagaaattca tgcatgcaag gccaaggaat agaccagagg gcacggctct ccgcaagatc
     3541 cacgactatt ttgtaagcgc cctcatgcca gggcgaatcc tcacggtggg cgtacaccaa
     3601 aatattcgtg tcgacagcga tcatccgccg tggccttcgt aggccatgtc acgaatttgt
     3661 tcccacgtcg ccgtcgacac tccggattga agcccctggc ctttgaaggt ggcctttctt
     3721 aggcgaaaga cacctggctt tttttttgtc gagataatct ggcgcaggcc ctgttcgatc
     3781 aaggccttga cggtggtgcg ctcccgggcg gcgagccttt ttgcctcgtc cagcagatta
     3841 tcggatatct ctacggttgt tttcatatgg gtatatgtat catatgatat ggttatacgt
     3901 caattaatca atttccgagt atttacggca gtcaagtcga gcggcacgcg tcaattgacc
     3961 tgccaattgc tgaccgaact cgccgacggc tatcgttcgg ccgttgtgac accggatcgt
     4021 gccgatctca tcaaatacgc gcgcaaatag ctcgcctcga aacttcttat tctcgagcag
     4081 tccatgggtg gttgcgaaat ggagccggcc ggtttcgcaa agccgcttca acaagtcgaa
     4141 accgatttgg cttcgcgagt tcagcccctg ctggttcatc agctcgaata cgttggtttc
     4201 tccatcgccg ggcagcccct tgagataatt tacgatccgg gtcgtcacga acagcgtgta
     4261 aatatcgtcg cgcgtgaaat ggtcggtttc gatggcgaga gcggtaagcc gtgctttaac
     4321 aaaatcgcct tcggtcaatt ttaaaccgcg ggcgatgggc gagttcggta ttcgatagaa
     4381 cggcgaggcg ccgagcaaca ctggcaggcg cgcgttgaat gccagagttt ggatcatgct
     4441 gtccaaagat tcgttcggca acccgaggat ttggtacgac acaatcttaa agccgagttc
     4501 atgggctttg ctgacgacct tcacataggc ttccaaggta tgcggtcgct tggtggtctc
     4561 acgaacggtt ttgtcggagc taaccagggc caaattcaaa tgagaaaatc ccgcctggtg
     4621 cattaattcc aagagttcat cgtcgaggct cagataactg atgccgttca tggcgacgaa
     4681 ttgcatctcg cgattgggaa accgcgctat gagccggcgg catagctgtt taaacgtctg
     4741 cttgtaatag gtgaggttgt cgtcttcgaa gtcgataacg cggtaacctt gccggtggcg
     4801 cagctcgatc tcctccagca cttgctcgag cgaacggcgg cgatagtttg tgccgaaggt
     4861 cgagtgcacc gagcaaaacg agcatttatg cgggcagctg cgcgaagtta tcatgaaggt
     4921 caacggcttg ccggcgagtg tgtagctttg cggcgtcgcg tcgccgacgt ctggtaccgg
     4981 cagttcttcg atgggaaaat tgtctgcgat cgggttcagg acgaattcac cgcgatgctt
     5041 gtaagcgagg ttggcaaccg cttcgaccgg ctgttgatcg cgcagacagc gcaaaaattc
     5101 gaccaatggc cgttcgcctt cgctacgaat aacgtaatcg acatgcggag atgccagcag
     5161 cgattccgga gctgccgagg cgtgcgagcc gcccatgacg accatcgctt gtgaaacctt
     5221 ttttacccgc gctgctactt cgagtgcctc gcggaaatac gctgtgaaca gcgatgagat
     5281 accgacgacg tcaggcttca ataagcttat gtcttcctcg atggcgtcga agggcatgcc
     5341 gaagtgatag tagcgattaa aagtcgagaa tggcgacttg tccgcggcgc catagtagtc
     5401 gctcaaatag cgtagttctt tgggaatctg cacggtcttg cggccgacgc cgcggtgata
     5461 gtctctgacg ataacttcga tatccggaag atgcttgcta accgcagcct ttaaatagca
     5521 caggccaatc ggctgtaaac gcacgtcggt gtcgtagaag tcttgtaccg gcggttgaac
     5581 gagcaacact cgcatgtgac tggcgttata tcgcatatac tcggcgcgac ggaaacgatt
     5641 gtattgtcga gtcaacaata ttaaagattt ctaaaaagca ccatcgacgg aaaagtgcag
     5701 gagcgacgat gaagatcgaa aaaatcgaca gcaagaagct tgaaatgccc tacaccaagc
     5761 cgctggtgac ggcgaccaac aattttaccg tcgcgcgcgg gctgctcgtc accgtggtga
     5821 ccagcggcgg cgtggaaggg tatggctatg ccgatctttt tccacgcgcg ggcgagactc
     5881 ccgagaccgc ggcgcacgtc atcgagacgg ttctaaaagc caaggcgctg ggcaaggagt
     5941 tagaagacct cggccgcatt cgcgccgaga tggatcacgc tttgaccggt cacccgcgcg
     6001 ccaagtcagt attggagggc gcgatgtacg acgccctggc gcggagctat cacattccga
     6061 tgtcggtcat gctcggcggc aagtgtcgca acgagatcaa agtcatcaag atggtcagtg
     6121 tcggcgatcc cgaggcgatg gcggtggaag cgaaaggact tgcctctgag ggtttgtcgg
     6181 tgaaactcaa agtcagcggc cgcatcgaca aggatctgcc gcgggtcgcc gccgtgcgca
     6241 aggcaatcgg cgacgatgtc ttcatcaaga tcgatgccaa tgaagcgtac gatgccaaaa
     6301 ctgcgatccg gctcgccaag gcattggctg atcttggcgt cgaagtattc gagcagccgg
     6361 taccgcgcga tcaattcgac gcgttgtggg aagtaaaaaa acattcgccc atcaagattg
     6421 aagcggatca gtcctgccgc aattttgccg acgcacagat actggtcaaa aatcgcatgg
     6481 tcgactcgat caataccggc atccccaaag tcggcagtgt cggcgaagtg cggcgtatcg
     6541 ccgagctgtg cgaggccaac ggtatccgct gttccctatc caatacagcg ggaagtgtcg
     6601 ttggtgatgc ggcggcactc catctggcgg tgagcacgcc gggcatctca ccgttatgtg
     6661 aattgggtga gttcgaggtc atcaccggcg atccgttctt cggcctcgcc gtgactaacg
     6721 gcacactcga agtgcccgag ggtgaaggat tgggagtaaa tctgagaggg ttctgaagaa
     6781 tggagtgctg gggttcggaa tccactactc tgttaatgaa ttccatctcg gggatggtta
     6841 aaacctctcg cttgaggcga gtggagattt gtcatggcgg cgaaggcccg catccatctt
     6901 tcggatcgct gcaaggtcaa agaaaacctg gattccggct gcgccggaat gaccgaaaga
     6961 aatgccgact tttttcaggc aacaatttgg aactgctcgg ctcagagccg aggaacgtga
     7021 gttcgctccg catgggaggt tttgatgagt tcagcttttg ccgcggcgat ggataagatc
     7081 gtcgccgact ataatgatcg actcgagtgg gtgcgtgctt cgaaatacga tttgaccaag
     7141 gagtcggcgc gggtgttgat cgagcagtgg tcgagtttca cccggcacag ccgacagtgt
     7201 tgggcttatg tcgtcggcaa ctgtcctcat gtcgaagtgc gcaagttcgt cgtcaccgag
     7261 aatctctacg aggaagaagc tctggaaggc cattctcatt tcgagattct cgcgcgcatg
     7321 ggtatcgctt tgggattgac ccgggatcag attgtcaacg ccaaaccgtt gccgacgacg
     7381 gtggtggcga tgcgcgcgtg ggaggctttg accaagaat
//
LOCUS       DATLCH010000208         4646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2053226, whole genome shotgun sequence.
ACCESSION   DATLCH010000208 DATLCH010000000
VERSION     DATLCH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053226"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VMZ02_06205"
     CDS             <1..770
                     /locus_tag="VMZ02_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71559.1"
                     /translation="TKGGRRVADEMELLNKTYGVKALTFMDDDFNSDRRKMVDLVEAL
                     EEKKLDISWFMLSRAQNLIRDMDLVPRLRKVGLYQVLIGIDGGTDEEIAEARKGPMKV
                     GVKELKELIQFLRKNDISTISTYLNGFWEDDEAKIRQRAKAVDEIDPDIVMIQVLNPI
                     PGSPIYKSAVKNGIIEIDNLSLYDLEHSVMPTKHLTRQQLGELTGWAFQSFYSKPGRI
                     ERILNGYTSPYVRMKFESFKNNAAKYEKNAAEGEVAI"
     gene            785..1561
                     /locus_tag="VMZ02_06210"
     CDS             785..1561
                     /locus_tag="VMZ02_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HUR71560.1"
                     /translation="MAEQNGRLPSLHGKVAVVTGASRGIGAAAAIRLAQGGAAVVVNY
                     HQNHEAAQKVLNQVEATGGRGIIFQADVTQRSQVDSMVKSARDTLGAIDILVNNAYFP
                     FEVSPLHEIAWDKFHRAIKHELAAFYHCVHACLPDMKEKKAGRIVVISTRLAQQPLPR
                     MGAYAAAKSALESMANTMAIELGPLGIAVNVVTPAFTLTDASLIMPEAYRERVKETRP
                     LKKHLYPEDVAGAIAFLAGDEASMLTGSHILVTGGSHLQL"
     gene            1672..3156
                     /locus_tag="VMZ02_06215"
                     /pseudo
     CDS             1672..3156
                     /locus_tag="VMZ02_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            3238..4032
                     /locus_tag="VMZ02_06220"
     CDS             3238..4032
                     /locus_tag="VMZ02_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71561.1"
                     /translation="MGLGRQDFSGKKLAPQEADAVIADLKKYLLEIADKWLIKGRFHT
                     DMGNGTLPMEAIKIFWQNWYGFVAEINNFHGVAYQRQQPFFKRHPELLKHFAAHVADE
                     MIHPKPPGHIQIVLEQGHNFGLADADMVEYEMLAECRAFLEYYRGVLYEGTISEWWAS
                     FCVEEAVGHWARFFGTALRKAYQFPEEKIVYFRVHAKADLEEHDGIMAHADLDWLVLK
                     NLLVEGKAETRPGFNLEYCMRMGTAYFAWFFEGVYQQIKKEGLYKE"
     gene            4035..>4646
                     /locus_tag="VMZ02_06225"
     CDS             4035..>4646
                     /locus_tag="VMZ02_06225"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HUR71562.1"
                     /translation="MAESNKEEVSPRPLMRILGDFANSQILDAALEYDFFTLISKGFD
                     SAEAVAREAGTDARATRIVLDSLPALSLVEKRAGRYLLTPSAQAFLVKGKPSYCGDFR
                     HVALALWDGMAHLKESLKTGKPLSRMDTNAELPVWEKLVLGIIVIAEPAAKALCDILQ
                     LGSERKGLRVLDIAGGSSIFGMTIVTRDPTAQVTQLDWPNVNAV"
BASE COUNT         1106 a         1222 c         1310 g         1008 t
ORIGIN      
        1 gcaccaaggg cggcaggaga gttgccgacg agatggagct gctcaataag acctacggcg
       61 tcaaagcgct cacttttatg gacgacgact ttaactccga ccgccggaag atggtcgact
      121 tggtcgaagc gttggaagaa aaaaaattag acattagttg gttcatgctg agccgcgcac
      181 agaacctgat tcgagacatg gatttggtcc cgcggctacg caaagtaggt ttgtatcagg
      241 tcttgatcgg tatcgacggc ggcacggacg aggagatcgc cgaggcgcgc aaaggaccga
      301 tgaaagtcgg tgtcaaggaa ttgaaggaac tgatccagtt cctgcgcaag aacgacatct
      361 ccaccatctc tacttatctc aacggttttt gggaagacga cgaagccaag atccgccagc
      421 gcgccaaggc agttgatgag attgatcccg atatcgtcat gattcaagtg ctcaatccga
      481 ttcccggctc gccgatctac aagtcggcgg tgaagaacgg catcatcgag atcgacaatt
      541 tgagcctata cgatcttgaa cacagcgtta tgccgaccaa gcacctgacg cgccagcaat
      601 tgggtgagtt aaccggttgg gcattccagt ctttttacag caaacctggc cgcatcgaaa
      661 gaattttgaa tggctacacc tctccgtatg ttcggatgaa atttgaatcg ttcaaaaaca
      721 acgccgccaa gtatgaaaag aacgcggcag aaggcgaagt tgcaatctag tagttggcgt
      781 tatcatggca gagcaaaacg gacgacttcc ttctcttcac ggcaaagtcg ccgttgtgac
      841 cggcgcgagc cgcggcatcg gagccgcggc ggcgatacgc ctagcgcaag gcggcgcggc
      901 ggtcgtcgtc aattaccatc agaaccacga ggcggcgcaa aaagttctca accaagtcga
      961 agcgaccggc ggccgcggga taatctttca agccgatgtt acgcagaggt cgcaggtcga
     1021 ttcaatggta aagtccgccc gggatactct cggcgccatc gatatcctgg tcaacaatgc
     1081 ctatttccca tttgaagtaa gccccctcca cgagatcgcc tgggataagt tccatcgggc
     1141 gatcaagcac gagctggcgg cgttttatca ctgcgtgcac gcctgcttgc cggacatgaa
     1201 ggagaagaag gcaggccgaa tcgtcgtaat cagcacgcgc ttggcacagc agccgctccc
     1261 gcgcatggga gcctatgccg cggcaaaatc ggccttggaa tcaatggcca acaccatggc
     1321 catcgagttg ggtccgctgg ggattgccgt gaatgtcgtt actccggcat tcacattaac
     1381 cgacgcctcg ctgatcatgc cggaagccta ccgcgagcgg gtgaaagaaa cccgcccgct
     1441 aaaaaaacat ttgtatcccg aagatgtcgc gggcgcgatc gcctttctcg ccggtgatga
     1501 agcgagcatg ctcaccggca gtcatattct cgtaaccggc ggcagccatc tgcaactgta
     1561 ataattaaaa ttgacgatac acaatcagat ttgttgccaa tcagatagtg tgcgtctata
     1621 acccaacttt gattttcgat tcaccgttct ccggtttcga ttcgcgttgc catgcttacc
     1681 attgacgaga tcattgcgaa gctgaccgac attgtcggtg cgcggcggat ctcgaccaat
     1741 cccatcgaca aaataaatta tcagcatacg ctggcttacg gcgcctaccg agccttgcct
     1801 gacgtaatcg tgcgcatcga gtccgacaac cagggcaaac acaaggttgc cgacattctt
     1861 aaactcgcta acgagcataa aattcccatc gtcgccaagg gcggcgtcgg catgggcatc
     1921 agttcgcccc tccagggtgg tgtgctgctc gatatgctcg gcatggataa gattctcgaa
     1981 gtgaatccgg acatgcagta tgtaatcgct gagggcggcg ccagtgttta tggaattcac
     2041 cacgcgttac gccaacatgg attgatgctg ccgaacttcg gtacctaggg cccggcggtg
     2101 gtcatcgggg cgatgatcaa taagggtggc atcggctacg gcatgacgcg ttacggctgg
     2161 attgccgata tggtcctggg cttggaagtg attctcgcga gcggggagac cattcgtctc
     2221 ggtgcgctgg cgaatcaggg cactactttc ggaccgtttc agaaatggat tcacctgccg
     2281 gacctgttgg gcttatttac cctggccgcc ggctccatgg gtattatcag caaagtttgt
     2341 ctgcgcgtga ttcggtgccg gcgcgaatgg ctgcacgatt actgttacgt ttttcgtcgc
     2401 gatcagctgc agcaggtgca ggattccctg attgaattgg tccgtgacga ggtcgtctac
     2461 gatatccatt ttaccgaccg ttggcagtat cactggccga tggaagaggg actgtacgat
     2521 aaagacaaaa ttcccgacga cggctggttt ttcttaaaaa ccgttctgat ggcgcgtgac
     2581 caagaagaat tggggtttaa agaaaaacgc atgcggcgga tctatgagcg cgcgggcgcc
     2641 gtcgacgtgc cggagatcgc cgacaaggcg atgggagcca aggcgcagga actggggctg
     2701 catggctggc ccgactggca cattatggga cccgacggtt ggtgcggtac cgtggtgcgc
     2761 cataccggta tgttcgcggt cacatcaaaa atgtatccgc tgcgttttct taaagagatg
     2821 tacgatctcg acgaggcggc caagcgcgag tgcggtatct gggacgccga acattcgcca
     2881 cagcatgata gttacgtaac ccgcgacttg gcgatcaaag aggagtactt tactttttac
     2941 aatccctatg acgatgaaga cgtcggccga ctgcgcaaat ggatggggat ggtcgcacag
     3001 ttcagcaacg agcgaggcgt agtctggact gcgccgactc tgagcactaa gggtccgctg
     3061 ccctacaatc gcttgggcag cctttacgat gtcgtcaaag agattaagca gctagtcgat
     3121 ccaaataata ttctgaatcc gggagcgcta ttctaaaccg tttcaaaagt ttcagcgttc
     3181 aacgcttcaa acggattcgg agacccgcac ataataaaaa atagctagga gagctttatg
     3241 ggattgggca gacaggattt ttccggtaaa aaactcgcgc cgcaggaagc cgacgcggtg
     3301 attgcggact tgaaaaagta tttactcgag attgccgaca aatggctgat caagggccgc
     3361 tttcacactg atatgggcaa tggcacgctg cccatggagg cgatcaaaat cttctggcaa
     3421 aactggtatg gatttgtcgc cgagatcaac aattttcatg gcgtcgctta tcagcgccaa
     3481 cagccctttt tcaagcgtca cccggagctg ctcaagcatt tcgccgccca cgtcgccgac
     3541 gagatgattc atcccaagcc gcctggccat attcaaatcg ttctcgaaca gggccacaac
     3601 tttggtctcg ccgacgccga tatggtcgaa tacgagatgc tcgccgagtg ccgcgctttc
     3661 ctcgagtact atcgtggcgt gctctacgag ggcacgatct ccgagtggtg ggcgtcgttc
     3721 tgcgtcgaag aagcggtagg ccattgggcg cgatttttcg gtacggcgct gcgcaaggcg
     3781 taccaatttc ccgaggaaaa gatcgtctac ttccgcgtcc acgccaaagc ggatctcgaa
     3841 gagcatgacg gtattatggc gcacgcggat ctagattggc tggttttaaa gaacctgctc
     3901 gtggaaggta aagccgaaac gcgcccgggc ttcaatttag aatactgtat gcgcatgggt
     3961 acggcatatt ttgcctggtt cttcgagggg gtttatcagc agatcaaaaa ggaaggtctc
     4021 tacaaagaat aaatatggcg gagtccaata aagaggaagt ttcaccccgt ccgctgatgc
     4081 ggatcctcgg cgatttcgcc aatagtcaaa ttctagacgc agcgctggaa tacgattttt
     4141 tcacgctgat aagcaaaggt ttcgatagcg ccgaagcagt ggcgcgcgaa gccggcaccg
     4201 acgcgcgcgc aacgcgcatc gtgctcgaca gtttaccggc gttgtccttg gttgaaaaac
     4261 gcgccggccg gtatctcctc acaccctcgg cgcaggcttt tctggtcaaa ggcaagcctt
     4321 cctattgcgg cgatttccgc catgtcgcct tggcgttatg ggacggcatg gcccatctca
     4381 aagaatcgct taaaaccggc aagccgctga gccgcatgga taccaatgcc gagctgccgg
     4441 tttgggaaaa acttgttcta ggcatcatcg tcattgccga gccggcagcc aaagcgctgt
     4501 gcgatattct acaactcggc agcgagcgca aggggctgcg ggtgctcgac atcgctggcg
     4561 ggtcgagcat cttcggcatg acgatagtga cgcgcgatcc gaccgcgcag gtcacccaac
     4621 tcgactggcc taacgtcaac gcggtg
//
LOCUS       DATLCH010000209         3743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5779165, whole genome shotgun sequence.
ACCESSION   DATLCH010000209 DATLCH010000000
VERSION     DATLCH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5779165"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..1889
                     /locus_tag="VMZ02_06230"
     CDS             366..1889
                     /locus_tag="VMZ02_06230"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUR71563.1"
                     /translation="MRYGFLIDQHRCIGCHACTVACKEEHNIPVGVNRTWVKYVEKGI
                     YPDTRRHFAVLRCNHCDDAPCIEICPTVALFRRPDGIVDFDNERCIGCKSCMQACPYD
                     ALYIDPDRNTAAKCNFDASRVEMGYKPACEVVCPTQAILSGDLDDANSTISKRIAMEK
                     VSVRKAEKGTKPKLFYAGVDGDLLNPNMMEPQETHFWADKDPGEDLYALRMATNERTT
                     PGAAREVYDVPHETPWGKKIASYLWTKSISAGVLLLSALLLNMGFEQDAGVLSLVSPA
                     VSLIFLGATMVLLVLDLKKPSRFFYILTKPNLNSWLVLGGYVLTFFGIFLCVWLGLIL
                     SGNGIHPVLIWTTATFAVASAGYSAFLFAQARGRDFWQSPLLFWHLLIQAVAAGAAML
                     TLIGSVLGVTLPLFSWLGHLLVISLSLSLAMIFGELFMKHGAEDAVRAGEMLVRGPLK
                     KSFWILVVIVGTLLPIVLVLWPISSLLPNVAAALLALFGLWMYEHLWVRAGQAVPLS"
     gene            1982..>3743
                     /locus_tag="VMZ02_06235"
     CDS             1982..>3743
                     /locus_tag="VMZ02_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUR71564.1"
                     /translation="MIEPRLPLNPDALKLEPNGLVNYPPVEKWDDWMEYDTAQWPRKV
                     EKHCRIIPTICFNCEAACGLVAVVEKDTNKIRRFEGNPEHPGSRGRNCAKGPATLNQV
                     TDPERIRYPLKRAGKRGEGKWERVSWDEVLDDIAGRVRKALLEERRNEIMYHVGRPGH
                     ELLYHQRILHSWGIDGHNSHTNVCSAGARAGYAFWSGIDRPSPDHANARFILLLSSHL
                     ETGHYFNPHAQRIIEAKEAGTKICVIDTRLSNTASKADYWMSTWPGSESAVLLAMCNV
                     LLQEDLFDREFLRRWVNWEEYLREEHPDRQVSLASFVAVLKEVYAQYTPEFAAKEAGV
                     EPGVIVDVAHEIARAGSAFAAHVWRNTAAGNLGGWQVARALQFLSVLVGAVGTVGGTA
                     PNAANKFIPAPPLMPPPSKVWNELLIPKEFPLAFFEMSFLLPHFIKEGRGKLAMYFTR
                     VYNPVWTNPDGMSWIEMLSDESKVERHACLTPTWSETAWFADYVLPMGHGSERHDLMS
                     QETQAARWIGFRQPILRVALEKQGKKFATTYEAHEAAGIGQVWEEDEFWIELSWRVDP
                     DGALGIRKYFESPYEPGKKMT"
BASE COUNT          791 a         1011 c         1083 g          858 t
ORIGIN      
        1 ccattcacca cgagccgcga aggcttcggc aatttgaaat actgaatttg gggtgtgttc
       61 ttccaaaaat gcatgcgcag ctacgaaccg gccgttgcgg gggaaaattc tgtcataccg
      121 gcgggaagcc ggtatcgccc ctccctggat gccggcctac gccggcatga cgagtgaaag
      181 aagaagcatt tggaaaggtg caaattcata ttctctaaac gagtgtcacc tcatgaatac
      241 tacgcagtga gtcatttcca agaaattttc tccgttcaat tgacccaagc tgaatagacc
      301 tgtaatcaga gtcgaaagtt ttacttacaa tacatagcta agttagttga tcggaaaggt
      361 ttataatgcg ttacggattt ctcatcgatc agcatcggtg catcggttgt cacgcttgca
      421 ccgtcgcctg taaagaggaa cacaatattc ctgtgggagt taatcgtact tgggtcaagt
      481 acgtcgagaa ggggatttat cccgacacgc ggcgtcactt tgcggtgcta cgctgtaatc
      541 actgcgacga cgcgccgtgc atcgagattt gtcccaccgt ggcgttgttt cgccgtcccg
      601 acggtattgt cgatttcgac aacgaacgct gcatcggctg caaatcctgc atgcaggcct
      661 gtccgtacga tgccctctac atagatcccg atcgcaacac cgcggcaaaa tgtaacttcg
      721 atgcttcgcg tgtcgagatg ggctataagc cggcttgcga agtcgtctgt ccgacacagg
      781 cgatcctctc cggcgatctt gacgacgcca acagcacgat cagcaaacgc atcgccatgg
      841 agaaagttag cgtgcgcaaa gccgagaagg gcaccaagcc aaaactcttt tatgcaggtg
      901 tcgatggcga tctgttgaat ccgaacatga tggagccgca agagactcat ttctgggccg
      961 acaaggatcc cggcgaagat ctctacgcat tgcggatggc gaccaacgag cgaaccacac
     1021 ccggcgcggc acgcgaggtc tacgatgtgc cgcacgagac cccttggggc aaaaaaatcg
     1081 cgtcctatct ttggactaaa tcaatttccg ccggtgtgct tttgttgtcg gcgctgttgt
     1141 tgaacatggg cttcgagcag gatgccggag tattgagttt ggtcagtccg gcggtctcgt
     1201 taatttttct cggcgcaacc atggtgttgt tggtgctcga tctgaaaaaa cccagtcgct
     1261 tcttttacat tctcaccaag cctaacctca attcctggct cgtgctgggc ggatatgtcc
     1321 tgacattttt cggcattttc ctgtgcgtct ggttagggct cattcttagc ggcaatggca
     1381 ttcatcccgt attgatctgg acgacggcga cctttgctgt tgcctcggca ggctattccg
     1441 cttttctctt cgcccaggcg cgcggccggg acttttggca gagtccattg ctgttctggc
     1501 atctgctcat tcaagccgtc gccgccggcg cggcaatgtt gactcttatc ggcagcgtgc
     1561 tcggtgtcac gttgccgctg tttagctggc ttggtcattt gttggtgatt tcgttgagct
     1621 tgagtttggc gatgatattc ggcgaattgt tcatgaagca tggagccgaa gatgccgtgc
     1681 gcgccgggga gatgctagtg cgcggaccgc tgaagaaatc gttttggatc ttggttgtaa
     1741 ttgttggaac gcttttgccg atcgtcttgg tgctttggcc gatcagttca ctgcttccca
     1801 atgtcgctgc cgccttgttg gccctcttcg gtctatggat gtacgagcat ttgtgggtga
     1861 gggccggcca ggccgtgccg ttgagttaat tgccagcaag ccgtgatcag tcgacgctaa
     1921 gaaatattcg tctccggctg agaactgctg gcaaaccgct ttcgaggagg gatcgaaata
     1981 catgattgaa ccgcgcctgc cgctgaaccc cgacgctttg aaacttgagc cgaacggctt
     2041 ggtcaactat ccgcccgtcg aaaaatggga cgactggatg gagtacgaca cggcgcagtg
     2101 gccgcgcaaa gtcgagaagc attgccggat cattccgacg atctgtttta actgcgaagc
     2161 cgcctgcggg ctggttgctg tggtcgagaa agacaccaac aagattcgcc gcttcgaggg
     2221 aaatcccgaa catcccggca gtcgtggccg caattgcgcc aaaggtccgg cgacactcaa
     2281 tcaagtgacc gaccccgagc gcattcgcta cccgttgaag cgcgccggca agcgcggcga
     2341 agggaaatgg gagcgcgtca gttgggacga agtgttggac gacattgccg ggcgtgtgcg
     2401 caaggcgctg ctggaagagc ggcgcaacga aatcatgtat catgtcggcc gccccggcca
     2461 cgagctgctc tatcaccaac gcattcttca ttcgtggggt attgacggtc acaacagcca
     2521 caccaacgtg tgttctgccg gggcgcgcgc cggttacgcc ttttggtcgg gcatcgaccg
     2581 gccgtcgcct gatcatgcca atgcgcgttt cattttgctt ttgagctcgc atttggaaac
     2641 cggccactat ttcaatccgc acgcccagcg catcatcgag gcgaaagagg cgggcaccaa
     2701 gatctgcgtc atcgacacgc ggctctccaa caccgcatcg aaagccgact actggatgtc
     2761 cacctggccg gggagtgagt ccgcggtgtt gctggcgatg tgcaacgtgc ttttgcagga
     2821 ggatctgttc gaccgcgaat ttctgcgccg ctgggttaac tgggaagagt atctacgcga
     2881 agaacatcct gacagacaag taagtttggc gagcttcgtc gcggtgctca aagaagttta
     2941 cgcccagtac acccccgagt tcgccgctaa agaagccggc gtcgaaccgg gcgtgatcgt
     3001 cgatgtagcg cacgagatcg ccagagcggg ttcggcgttc gctgcccatg tctggcgcaa
     3061 cactgcggcc ggcaatctcg gcggctggca agtcgcccgg gccttgcagt tcctcagcgt
     3121 cttggtaggc gcagtcggta cagtcggcgg tactgcgccc aacgcggcga ataaatttat
     3181 tcccgcgcca ccgctgatgc cgcccccgtc caaggtttgg aacgagctgt tgattcccaa
     3241 agaatttccc ttggcctttt tcgagatgag tttcttgttg ccgcacttta tcaaggaagg
     3301 gcgcggcaag ttggcgatgt acttcacgcg agtctacaac ccggtgtgga ccaatcccga
     3361 cggcatgagc tggatcgaaa tgctgtccga cgagagtaaa gtcgaacgcc acgcttgttt
     3421 gacgccgacc tggagcgaga ccgcgtggtt tgccgactat gtgctgccca tggggcacgg
     3481 ctctgagcgc cacgacctga tgagccagga aactcaggcg gcgcgctgga tcggctttcg
     3541 ccagccaatt ctgcgggttg ccttagaaaa gcagggcaag aaattcgcca cgacttacga
     3601 ggcgcatgag gccgccggca tcggtcaggt gtgggaagaa gacgaatttt ggattgaact
     3661 atcctggcgc gtcgatccgg acggagccct gggcattcgt aaatatttcg aatcgccata
     3721 cgaacccggg aaaaagatga cgg
//
LOCUS       DATLCH010000210         3161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1313041, whole genome shotgun sequence.
ACCESSION   DATLCH010000210 DATLCH010000000
VERSION     DATLCH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313041"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VMZ02_06240"
     CDS             <1..778
                     /locus_tag="VMZ02_06240"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HUR71565.1"
                     /translation="IVLAFGALGWIMEKLDWPRPPVLLGLVLGPLAENRLFLSTDNYG
                     LAWMHRPGVIGIFVLTLFGIFYPIIKDRRLAREKAKSTDRGAPVIDGDELAPTAARFG
                     PAVWFTVAVITTLAVALWQSRNFGFRAGLFPWVIGIPTLILAVAQLGRDIYGKKKHKV
                     AEYGESMETKIAPADVKQRTISIILWTIGFFVAIWLLGFSIAVPLTILLYLKIAGKES
                     WKMTALVTFFAWLFYWSLFEKLLNVPFHEGLLIGLIRGSQ"
     gene            complement(870..>3161)
                     /locus_tag="VMZ02_06245"
     CDS             complement(870..>3161)
                     /locus_tag="VMZ02_06245"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HUR71566.1"
                     /translation="TETYKVIGTAQLRNDGAEKVTGTALYTVDVDLPGMVHGKILRSP
                     YPHARLLRVDGSKAEQLPGVFAVVTREDQKSLRMFGAAYKDQTIVAVDKVRYAGDPVA
                     AVAAIDEATAEQALEMIEVDYEALPAVTSIDEALADNAPLVHDASARGGELMGQRYEA
                     PKEFSGTNLCYRFSYANGDADGAFTKADHIFENTFTFPRVQHFSMEPHATVAHFAGDR
                     ITLWAGTQEPFTLREHLADIFHLPQSKIRVIVPYVGGGYGGKLAVKTEPLAAVLSWKA
                     KRPVRLAHSIEESFKTVTRHPARVQIKTGVSKDGKLVARQCVIHMETGAYADAGPRVT
                     QKAGYRCFGPYRIPHMKTDAYTVYTNTVPAGAYRGFGTLQVTWAYESQMDIIAEKLGL
                     DPLEFRIKNLLKKGDLYTPGDTPVDCDLKAGLSQAAEEIGWKQKSTKPNRGRGLAVCM
                     KDGGGTYKVSSAAVKMNADGSVVLLTGTVEVGQGARTALSQVVAEELTIPFDAVTVAQ
                     LDTEITPYDVNTNASSSMVVMGLSVQRAAQDLKRQILRHAAKVLQVKTAQLQLSGGKI
                     VGAANLSLSFIDLMQKIFLSRSGELVGRGAYQSIKSSKAALGSPTNFWEISWGAAEVE
                     VDRDSGEITLVKYVSLADVGRAIHPVQCEGQDEGGVMNAIGHSLMEEMIYKDGQLLNP
                     NLIDYRVPSFATLPKDFETILVESHNGPGPFGAKGTGEGGLLPVAAAIGNAVFAATGL
                     RLCDLPLTPERVWRGLAAQRMTD"
BASE COUNT          654 a          928 c          857 g          722 t
ORIGIN      
        1 aatcgtgctg gctttcggcg cgctgggttg gatcatggaa aaactcgact ggcccagacc
       61 gccggtgcta ttgggactgg tgctcggtcc gctggcggaa aatcgcctgt tcttgtcgac
      121 cgacaactac ggtttagcgt ggatgcatcg accgggagtg atcggcattt tcgtgctcac
      181 tttgttcggc atcttttatc cgattatcaa agaccgtcgc cttgccagag aaaaagctaa
      241 atcgaccgac cgaggagccc cagttattga tggcgacgaa ctcgcgccaa ctgctgcacg
      301 attcggccct gcggtttggt tcaccgtcgc agtgatcacc actctagccg tggctctgtg
      361 gcaaagccgt aacttcggat tccgcgctgg attatttcct tgggtcattg gtattccgac
      421 cttgattctc gcagtcgccc agttgggccg cgacatttac ggcaaaaaaa agcacaaggt
      481 ggcggagtac ggcgaatcga tggaaaccaa aatagccccc gccgacgtca aacagcgcac
      541 gatttcaatt attctttgga ccatcgggtt ttttgtggcg atttggttgc taggcttttc
      601 cattgccgtg ccgctgacta ttctgcttta cttgaaaatc gccggcaaag agagctggaa
      661 aatgaccgcg ttggtgacgt ttttcgcctg gctgttttac tggagcctgt ttgaaaaact
      721 cctcaacgta ccgtttcacg agggtctgtt aatcggtttg atcagaggct cacagtaaag
      781 acaaacgttg ggctctcggg aaaaagcgct gcgaaatgcc caatctgttt tggctgggcg
      841 atttgatcac tcatcaaatc cattatccac taatccgtca ttcgctgtgc cgccaaaccg
      901 cgccagaccc tttccggcgt cagcggcaag tcgcataagc gtaagccggt cgccgcaaag
      961 acggcattgc cgatggcagc ggccaccggc aatagcccac cctcgccggt acccttggcg
     1021 ccgaagggtc ctggcccgtt atggctctcg accagaatag tctcgaagtc cttcggcaaa
     1081 gtcgcgaaac tcggcacccg ataatcgatc aaattgggat tcaacagctg cccatccttg
     1141 taaatcatct cttccatcag gctgtggcca atggcgttca tgacaccgcc ttcgtcctgg
     1201 ccttcgcact gcaccggatg gatcgcgcga ccgacatcgg cgagcgagac atacttgacg
     1261 agtgtgatct cgccgctatc tcggtcgact tcgacttcgg ccgcacccca gctgatctcc
     1321 caaaaattgg tcggcgagcc aagcgcagcc tttgaactct tgattgattg ataagcgccg
     1381 cgaccgacta gctcgccgga gcgcgacaag aagattttct gcatcaaatc gataaaggat
     1441 agacttagat ttgccgcgcc aacaatcttc ccgccgctca gctgcaactg agcagttttg
     1501 acttggagaa ctttcgccgc atgacgcaag atctgccgct tcaaatcctg cgccgcgcgc
     1561 tgcaccgaca accccatcac gaccatggag ctgctcgcgt tggtgttgac gtcgtaagga
     1621 gtgatctcgg tatccagctg cgccacggtg acggcatcga atggaatcgt caattcttcg
     1681 gcgaccactt ggctcaaggc agtgcgcgcg ccttgaccga cttccaccgt gccggtgagc
     1741 agcacgacac tgccatcggc gttcattttc accgccgcgg aagacacttt gtaagtgccg
     1801 ccgccgtctt tcatgcatac cgcgaggccg cgaccacgat tgggcttagt ggatttttgt
     1861 ttccaaccga tctcttcggc ggcttgcgat aggccggcct taaggtcgca gtccaccggc
     1921 gtatcgccag gcgtgtacaa gtcgcctttc ttaagcaaat ttttaatgcg gaattcgagc
     1981 ggatccaagc ccaacttctc ggctatgatg tccatctgcg actcataggc ccaagttact
     2041 tgcagcgtgc cgaagccgcg gtaagcgcct gcgggcacgg tattggtgta aaccgtatac
     2101 gcgtcggtct tcatgtgagg aatccggtag ggtccaaagc aacgataacc tgccttctgg
     2161 gtcacccgtg gaccggcatc ggcataagcg ccggtttcca tatgaatcac acactgtcgc
     2221 gccaccaatt tgccgtcttt acttacgccg gtcttgatct gaacccgcgc cggatggcgc
     2281 gtaacagtct taaaactctc ttcgatgcta tgggctaaac gcaccggccg tttggccttc
     2341 caggataata ccgccgccaa cggctcggtc ttgaccgcca atttgccacc gtaaccgccg
     2401 ccgacgtaag ggacgatgac gcgaatctta ctctgcggca gatgaaatat gtcggccaga
     2461 tgctcacgca gcgtgaacgg ctcctgagtg cccgcccaga gcgtgattcg atcgccggcg
     2521 aaatgagcga cggtggcgtg cggctccatc gagaaatgct gcacccgcgg aaaggtgaag
     2581 gtgttttcaa agatgtgatc ggccttggta aaagcgccat cggcgtcgcc gttggcatag
     2641 ctgaagcgat agcaaagatt cgtaccgctg aactccttgg gcgcttcgta acgttgcccc
     2701 ataagttcgc cgccgcgggc tgaagcgtca tgcaccaacg gcgcgttgtc ggccaaggct
     2761 tcatcgatac tggtcaccgc gggcaatgct tcataatcga cttcgatcat ttccagcgcc
     2821 tgttcagcgg tggcttcgtc gatcgctgcg accgcggcta ccggatcacc ggcatagcgc
     2881 actttgtcga cggcaacgat ggtttgatcc ttgtacgccg cgccgaacat gcgcaaactt
     2941 ttttggtcct cgcgcgtcac tacggcgaac acacccggca gttgctcggc cttgctgccg
     3001 tcgacgcgca ggagccgcgc atgagggtac gggctgcgta gaatcttgcc atggaccatt
     3061 ccaggcagat cgacatcgac cgtatacagc gcggtgccag ttaccttctc ggcgccgtcg
     3121 ttgcgcaact gcgccgtgcc gatgacttta taggtttccg t
//
LOCUS       DATLCH010000211         4329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6994598, whole genome shotgun sequence.
ACCESSION   DATLCH010000211 DATLCH010000000
VERSION     DATLCH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6994598"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VMZ02_06250"
     CDS             <1..667
                     /locus_tag="VMZ02_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HUR71567.1"
                     /translation="WAQPAGYVHALGEICRRHGILFILDEVATGFGRTGKMFACEHAG
                     VTPDILCLAKGITGGYLPLAATLTTEDVFAAFLGEYKQFKTFFHGHTYTGNPLGCAVA
                     IANLEVFAKEDIVGKMQPRVGVLQQRLHDEFLALTHVSDVRQWGYMIGIELVQDRANR
                     NSFPPEKRVGHQVILKARERSVMIRPLGDVIVLMPPLSMTDGELATLLDVVYDCIVTV
                     TGN"
     gene            668..1372
                     /gene="bioD"
                     /locus_tag="VMZ02_06255"
     CDS             668..1372
                     /gene="bioD"
                     /locus_tag="VMZ02_06255"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HUR71568.1"
                     /translation="MGQAIFVTGTDTGVGKTFFTCGLARWLKSYGYNVGAMKPAETGC
                     ELRDGLLYPEDGWRLKEATGCEEPIERVCPYRLPEPLAPSIAAERAGLKIDIDHLLAV
                     FAEIKRSHDITLVEGAGGLMVPLVPSYTFADFARVAKLPTIVVAANKLGVINHLLLSL
                     EHASCMGLTVLGYVLNRSINEQSVAAETNREVLLGLTGVPCLGELPFHQPGSDPANLF
                     ETELDLTTIHLFLEKL"
     gene            1369..2328
                     /locus_tag="VMZ02_06260"
     CDS             1369..2328
                     /locus_tag="VMZ02_06260"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71569.1"
                     /translation="MKTRLNAFVLVLALLFSASALGADKVIISYSSRSYAFLPAQIAV
                     ARGFFKDDNLEPLLIQMRSQVTVPALLSGEVHYTLSFGNIIGSAMAGLPFKILGVLTD
                     KPLHSIVARPEIKTIADLRGKRIGSQRIGGSDQLAAEAILQARGIDLKDVQFITLGGD
                     EPVRTEMLRKGLVDAICTVPPGPVRLAREGYNVLGGPKDLKVGSPISAVAVTDTRLKT
                     HREETKKVLRAVLRGLRIMHERRDDTIAIMSKWLNQSADVARDSYDSILPSFSLDGGT
                     TDKTYEFAIDSRKASVRADKPIPLSQVRDLSLLREVQKELRSQ"
     gene            2702..>4329
                     /gene="ppk1"
                     /locus_tag="VMZ02_06265"
     CDS             2702..>4329
                     /gene="ppk1"
                     /locus_tag="VMZ02_06265"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HUR71570.1"
                     /translation="MDANTYINRELSWLEFNRRVLEEAKDESVPLLERVKFLAIFSSN
                     LDEFFMVRVARLKAMLFEADNSCDPDGLAPAEALTAISARVHELAAEQHLCFLNTIMP
                     QLSAEGIHLVRPEEMSGEQERFLEEYFLRTLYPIVTPLAIDPAHPFPYLPNRSLGLVV
                     SLRPLTASRLPHTDLSIVHIPTQVAPRFITLPARDGQHAFMLLEDTLHHYLPRLYHGF
                     EIVSCHAIRVTRDADFLVSRRRDEDLMITIEKGVRERRMGDAVRLQYDPDLPLETVTQ
                     LVEELELTREDLYPGKGFTAFTDLFQLYSAVNLPRLKDRPQAPLPVPAFERASDIWSA
                     IRNGDVLTFHPYQSFDAVVRFVEEAAKDPKVLAIKMTLYRVSPTSPIAQALARAAEAG
                     KEVSVLVELQARFDEEANITWARALEQVGAHVVYGLVGYKTHCKVCLVVRQEADGIRR
                     YCHLATGNYNVRTAGVYSDVGLFTCSDTFGQDLTELFNLLTGYTRPLKFNHLILAPIN
                     MREFFISSIRREADHARSGHSARIIAKINSLVDPP"
BASE COUNT          918 a         1202 c         1208 g         1001 t
ORIGIN      
        1 gtgggcgcag ccggcgggct acgtccacgc gctcggcgaa atctgccggc ggcacggtat
       61 tcttttcatt ctcgatgaag tggccacggg cttcggccgc acaggaaaaa tgtttgcttg
      121 cgaacacgcc ggagtgacgc cggacattct ttgcctggcc aagggcatca ccggcggcta
      181 tttgccgctg gcggcaacgc tcaccaccga agatgtattt gcggcattcc tcggcgagta
      241 caagcaattc aaaacctttt ttcacggaca tacttatacc ggcaatccgc tcgggtgcgc
      301 ggtggccatt gccaaccttg aagtgttcgc caaagaagat atcgtcggca aaatgcagcc
      361 gcgagtaggc gttttgcagc agcgtttgca cgacgaattc ttggcgttga ctcatgtcag
      421 cgatgtccgt cagtggggct acatgatcgg catcgaattg gtccaagacc gcgcgaaccg
      481 gaatagcttc ccgccggaaa aacgcgtcgg ccatcaagta attctcaaag cacgcgagcg
      541 cagcgtgatg atccggccgc tcggggatgt catagtactc atgccgccat taagcatgac
      601 tgacggtgag ttagcgactt tgctcgatgt tgtatacgac tgcatcgtca cagtcacggg
      661 aaattaaatg ggtcaagcga tcttcgtcac cggcactgac accggagtcg gcaaaacttt
      721 tttcacctgc ggcttggctc gctggctgaa gtcttacggc tataacgtcg gtgcgatgaa
      781 gccggccgag accggctgtg agctgcgcga cggtttactc tatccagaag acggctggcg
      841 tctaaaggaa gcaaccggct gcgaagagcc catcgagaga gtttgtccgt accgcttgcc
      901 ggaacctttg gcgccgagta ttgctgcgga acgcgcgggg ttaaaaatcg atatcgatca
      961 tttgctcgcg gtgtttgccg agattaagcg ctcccatgac atcacgttgg tcgaaggcgc
     1021 cggcggcctg atggtgccgc tcgtgccgag ttatactttc gccgacttcg ctcgggtcgc
     1081 taagctgccg acgattgtgg tggcggcaaa caagctcggt gtcatcaatc acctattact
     1141 cagcttggaa catgcaagtt gcatgggctt aacggtactc ggatatgttt tgaacaggtc
     1201 gatcaacgag caatctgttg ccgcggaaac caatcgcgaa gtcttgctcg gtcttaccgg
     1261 agtgccctgt ctaggtgaac tgccgtttca ccagcccgga tccgacccgg caaatctctt
     1321 cgagacggaa ctggatttga ccaccattca tttgttctta gagaaattat gaaaacccga
     1381 ctgaacgcgt tcgttctcgt actcgctctg ctcttttcgg catcggctct cggcgccgat
     1441 aaagtcatca tctcctacag cagccgcagt tatgctttcc tgccggcgca gatcgccgtg
     1501 gcgcgaggtt ttttcaagga cgataatctc gagcccttgc tgatccaaat gcgcagtcag
     1561 gtgaccgtgc cggccctgct tagcggagaa gtccattaca cgctcagttt cggcaacatc
     1621 atcggcagcg ccatggcagg gttgcctttc aagattctcg gcgtactgac agacaaaccg
     1681 ctccacagca tcgttgcccg tccggagatc aagaccattg ccgatttgcg cggtaagcgc
     1741 atcggctcgc agcgcatcgg cggctcggat caactcgctg cggaagcgat tctccaggcc
     1801 aggggtatcg atcttaaaga cgttcagttc attaccctcg gcggcgacga gccggtgcgc
     1861 acggaaatgt tgcgcaaggg tttagtcgac gcgatctgca ccgtgccgcc cggaccggtg
     1921 cgcctggcgc gcgagggcta taatgttctg ggcggaccca aagacttaaa ggtcggcagt
     1981 ccgatctcgg ctgtggcggt gaccgataca cgtttgaaga cgcaccgaga agaaaccaag
     2041 aaagtcttgc gcgcggtgct gcgcggttta cgcatcatgc atgagcgacg cgacgacacg
     2101 attgcgatca tgagcaagtg gctaaaccaa agcgccgatg tcgcgcgcga ttcgtacgat
     2161 tcaattttgc cgtcgtttag cctcgacggc ggcactactg acaagaccta cgagttcgcc
     2221 atcgattcgc gcaaagccag cgtccgcgcc gataagccga tcccgctgtc gcaagtgcgc
     2281 gatctatcgc tgttgcgcga ggtacaaaaa gaattgcgct cgcaatgatc cagccgggcc
     2341 gatctaaacc gaaactaagc catctcgatt acccgtcgcc ggtatggttc ttacctgttc
     2401 gttaactcgc cgttaaccgt tcgtttacaa gcccggaata ctaaatactc gcggctgagt
     2461 tgttatggct tcgcgagcgc agaaagcaaa tggcgagtag ccaaacaact cgtcactgcc
     2521 aggtctattc ggcattcgag aactccataa tcgagagtga cagtcggtgg aaggtcataa
     2581 ttagaccggc gagtggacgc gccattcctt agttgtgtta ccaagggttc acttgttaca
     2641 gttacctggt acggggtttc gtttttggcg ttccgcgcga ccggcattcg gagcgaatca
     2701 gatggatgcg aacacctata ttaatcgtga attatcatgg cttgaattca accgccgagt
     2761 cctcgaagaa gccaaagacg agagcgtgcc gctcttggaa cgagtgaaat ttctcgcgat
     2821 ctttagttcc aatctcgatg aatttttcat ggtgcgggtc gcccgcctca aagccatgtt
     2881 gttcgaggcc gacaacagct gcgatcctga tggcctcgcg ccggcggaag cgttgacggc
     2941 gatatctgcg cgagtgcatg aattggccgc tgagcagcat ttatgctttc tcaacaccat
     3001 catgccgcaa ctgagcgcag aaggaattca tctggttcgt cccgaagaga tgagcggcga
     3061 gcaagaacgt tttctcgaag agtatttcct gcgcaccctg tatccgatcg ttacgcctct
     3121 ggcgatagat ccggcccatc cgtttcccta tttgcctaat cgttctctcg gtctggtagt
     3181 gtccttgcgg ccactgacgg catcacgttt gccgcatacc gatctttcca ttgttcacat
     3241 ccccacccag gtggcgccgc gtttcataac tttgccggca cgcgacggac aacatgcctt
     3301 catgctgttg gaagacacct tacatcacta tttgccgcgc ctgtatcatg gtttcgagat
     3361 tgtttcgtgc cacgctatcc gcgtgacccg ggatgccgat ttcctggttt cccgccgacg
     3421 cgatgaggat ctcatgataa ccatcgagaa aggcgtccgt gagcggcgca tgggtgacgc
     3481 ggtgcggttg caatacgacc ccgatctgcc gctcgaaacc gtcactcagc tggtggagga
     3541 gcttgagctc acacgtgaag atctctatcc cgggaaagga ttcaccgcct tcacagattt
     3601 gtttcaattg tacagcgcag tcaatctgcc acgcctaaag gatcggcccc aagcgccgtt
     3661 gccggtgccg gctttcgagc gtgcctccga tatctggagt gcgattcgca acggcgacgt
     3721 cctgaccttt catccttatc agtcgttcga tgccgtcgtg cgttttgtcg aggaggccgc
     3781 caaggatccc aaggtcttgg cgatcaagat gacactctac cgcgtcagtc caacctcacc
     3841 gatcgctcaa gcgctggcgc gcgccgccga agccggcaag gaggtctcgg tactggtcga
     3901 attgcaagcg cgtttcgacg aggaagccaa tatcacttgg gcgcgcgcgt tggaacaagt
     3961 cggcgcgcat gtggtttatg gcctggtcgg ctataagacg cattgcaaag tgtgcttggt
     4021 ggtacgccag gaggctgacg gcattcgtcg ttactgccat ctcgccactg gaaattacaa
     4081 tgtgcggacg gcgggtgttt atagcgatgt cggtttgttc acctgtagtg atactttcgg
     4141 tcaagatctg accgagctgt tcaatttgtt gaccggatac actcggccgc ttaaatttaa
     4201 ccatttgata ttggcgccga tcaacatgcg cgagttcttc atcagtagta ttcgccgtga
     4261 ggcggatcat gcgcggagtg gacactcggc gcggatcatc gccaaaatca acagtttggt
     4321 cgatccgcc
//
LOCUS       DATLCH010000212         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2062383, whole genome shotgun sequence.
ACCESSION   DATLCH010000212 DATLCH010000000
VERSION     DATLCH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062383"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1174
                     /locus_tag="VMZ02_06270"
     CDS             353..1174
                     /locus_tag="VMZ02_06270"
                     /inference="COORDINATES: protein motif:HMM:NF020287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HUR71571.1"
                     /translation="MDSNYRTGPLAADEPVILLDRKDREYLWRLDQRRPIAIRGGTIA
                     VEDIIGHDEGIVVRSSVNEPFLVFRPSLPQLIPNLPRTAQVVYPKDLGPILIWADLFP
                     GARVVEAGVGAGALSMALLRAIGGAGELISYELREDFSEMAQQNVARYFGPSPNWTIK
                     LGNVATDLDESEVDRVVLDLPEPWHVIERAWRVLRPGGILLCYLPTVLQVKSLVDALR
                     DDKRFACIETSETLMRFWHFKGMSARPQHRMVAHTGFLTSAKRLADGQKDIPRAP"
     gene            complement(1261..1680)
                     /locus_tag="VMZ02_06275"
     CDS             complement(1261..1680)
                     /locus_tag="VMZ02_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71572.1"
                     /translation="MKVKQIYHIGLPCNDLERGVKFYTEVLGMKCTKIGYDTESGGPY
                     KDKYGMFPNIARLFMEDGMCLVLFQRPEEVERGDFDEGTSHLALEVSSEDFDKATEEL
                     KNAGVKVSIDKPVVRPTGKAIYFYDTEQNYIQLWSRP"
     gene            1805..2776
                     /locus_tag="VMZ02_06280"
     CDS             1805..2776
                     /locus_tag="VMZ02_06280"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71573.1"
                     /translation="MKRTLFVFVWSIWLFTSATAIAQSPLERIVITYPSRSIASVDLY
                     IAQERGFFRQEGLLADVVQVRGNIGVTALLSGDAHAINNVGTLIRAAERSDLPAKVLS
                     QSLKKNLFWLVTKPEIKSLAELKGKTFGTTTFGGSQHLAALRLLQKAGLDPEKDITVV
                     VGGDVPAQLQSLLSGVVQLVALSPPTVILARDKFKLRIHGSTLEDLANLQNGLAFSDR
                     LVRDRRELVKRILRARSRAHRYLFENERGTAEVLAKFLNVDLAVALESYRLARPAFTA
                     NGIPTDKEVEEFLRADAEVLKLKEPVPAAKIFDFALQREINQELGIK"
     gene            2821..>3476
                     /locus_tag="VMZ02_06285"
     CDS             2821..>3476
                     /locus_tag="VMZ02_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71574.1"
                     /translation="MNILITGAGLIGAHTARQAVDAGHKVVLFDLSPNRDYIQKVVGK
                     DKADVVAANMLDLPALLTALEKFNVDTIVHTAGLIGGRVQENSYTGATNNILGTINIL
                     EAARLRRLRRLVYVSTFGVYERGKITDNLVKELHPIGGHNLYATTKVCSEHLVHAYTS
                     MYNLDTVIIRPGGVFGRGFYVGGSTVGMIMRDLALAMIKGEPITIDPKTYGPNEYVYG
                     "
BASE COUNT          762 a         1016 c          940 g          758 t
ORIGIN      
        1 tcccaggaag tccgcgagat cgtcgacgaa ctgcatcgcc aaggcatggg cagctacact
       61 atcaagtaaa ttggactaat tctacgccac agatttctca acactccttc gaaaagacct
      121 gagcaccaag gggaacgaag agaggtcgaa ttccgtcata ccggcgaagc cgggtatcca
      181 ggcacggtgg ggtgccggaa acctggattc cggcctgcgc cggaatgacg attgaggggg
      241 agcatcgccg atgggggaga tccctcggtt ttcattctct gtgggcgagc gtaagctgat
      301 gaacacttcg tggtgaatcc ctatccgcaa ttaatccata acaacgggaa atatggacag
      361 caactatcgc acaggcccat tggccgccga cgagccggtg attttgctcg atcgtaaaga
      421 ccgggagtat ctctggcgcc tcgatcaacg tcgcccgatc gccattcgcg gcggcacgat
      481 cgcggtcgag gacatcatcg gccatgacga aggcatcgtc gtacggtcat cggtgaatga
      541 accttttctg gtcttccgtc cgagtctgcc gcagctgatt cccaacctgc cgcgcaccgc
      601 gcaagtcgtc tatccaaagg atcttgggcc gatcttgatc tgggccgatc tttttcccgg
      661 cgcgcgtgtc gtcgaggccg gggttggcgc cggcgcactg agcatggcgc tgctgcgcgc
      721 catcggcggc gctggggaat tgatttccta cgagctgcgc gaagactttt ctgaaatggc
      781 ccagcaaaat gtcgcgcgct acttcggtcc atcgcccaac tggacgatca agctcggcaa
      841 tgtcgccacc gatttggatg aaagcgaggt tgaccgggtc gtcttggatc ttcccgaacc
      901 ctggcatgtc atcgagcggg cttggcgggt gcttcgaccc ggcggcatct tactttgtta
      961 tttgccgact gtgttacaag tgaagagtct ggtcgatgcc ctgcgcgacg acaaacggtt
     1021 tgcctgcatc gagaccagcg aaaccttgat gcgcttttgg catttcaaag gcatgagcgc
     1081 gcggccacag caccgcatgg tcgcccatac cggatttctc acctcggcga aacgactggc
     1141 cgatggtcaa aaagacattc cccgagcgcc ctgaagcaat ttcaggaagc tcggggaatg
     1201 atgaagctac gttattaact ttggattacg aatggggatg gctggcaagt atttccaaac
     1261 ctagggcctg gaccagagct gaatataatt ctgttcagtg tcgtagaaat agatcgcctt
     1321 gccggtcggc cggactaccg gtttatcgat cgacactttc acaccggcat ttttcagttc
     1381 ctcagtggcc ttgtcgaaat cttccgagct gacctccagc gccagatgag aggttccctc
     1441 atcgaaatcg ccgcgctcaa cctcttcggg gcgttggaac agcactaaac acatgccgtc
     1501 ttccatgaac agccgggcaa tgttgggaaa catgccgtac ttgtctttgt acggaccgcc
     1561 gctctcagtg tcgtagccga ttttggtgca cttcatcccc aagacttcgg tgtagaactt
     1621 aacgccgcgc tccaaatcgt tacacggtag gccgatatgg tagatctgct ttactttcat
     1681 ggaatcgctc ctccttgttt accttttgta tactgtaact tcccgcgcgg tttcaagaga
     1741 cagccgcaat gcctgcctac gttcggttga catccacaac gattacacta aatatcgaag
     1801 ccaaatgaaa cgtactcttt ttgtttttgt ttggtcgatc tggcttttta ccagcgcgac
     1861 ggcgatcgcc caatcacccc tggaacgcat cgtcatcacc tatccgagcc gcagcatcgc
     1921 ttcggtcgat ctttacatcg cccaggaacg cggttttttt cgccaagagg ggctgctcgc
     1981 cgatgtcgtc caggtgcgcg gcaacatcgg tgtcacggcg ctgctcagcg gcgacgccca
     2041 tgcgatcaac aatgtcggta ccttgatccg cgccgcggag cgtagcgact tgccggcaaa
     2101 agtcttgtcg cagagtttaa agaaaaacct attctggctt gtgaccaaac cggaaataaa
     2161 atcgctcgcc gagctcaaag gcaaaacctt cggcaccact accttcggcg gctcgcagca
     2221 tctggccgcg ctccggcttt tgcaaaaagc cggcctcgat cccgagaaag acatcaccgt
     2281 cgtggtcggc ggcgatgtgc cggcccaact gcagagtctg ttgagcggcg tcgttcagtt
     2341 ggtcgcgcta tcgccgccga cggtgattct ggcgcgcgat aagttcaagc tgcgcatcca
     2401 cggcagcacc ctggaggatc tcgccaatct gcaaaatggt ctcgccttct cggacagact
     2461 cgtgcgcgac aggcgcgaac tggtcaagcg aatcctgcgg gctcgctccc gcgcgcaccg
     2521 ctacttgttc gaaaacgaac gcggcacggc cgaagtgctc gccaaatttc tcaatgtcga
     2581 cctggccgta gccctcgagt cctatcgcct ggcgcgtccg gcatttacgg caaatgggat
     2641 acccaccgac aaggaagtcg aagaatttct ccgggccgac gccgaagtgc tcaagctcaa
     2701 agagccggtg ccggctgcaa agattttcga cttcgccctg cagcgggaaa tcaaccaaga
     2761 gctgggcata aaataatctt tcagcgtcat tcacgaaaag ttccgaggag gcaatctaca
     2821 atgaatattc tcatcaccgg cgccggtctc atcggtgcgc atacagcgcg ccaagcggtc
     2881 gatgccggcc ataaggtcgt gctctttgat ctctccccga atcgcgacta catccagaag
     2941 gtcgtcggta aagacaaagc cgatgttgtc gctgcgaata tgcttgattt gccggcgctg
     3001 cttaccgcgc tggaaaaatt caacgtcgac accatcgttc acaccgccgg attgatcggc
     3061 ggtcgggtcc aggaaaactc ctacaccggc gccaccaaca atattctcgg cacgatcaat
     3121 attctcgaag cggcacggct gcgaaggttg cgccggctgg tctatgtcag caccttcggc
     3181 gtctatgaac gcggcaagat caccgacaac ctggtcaaag agcttcatcc tatcggcggg
     3241 cacaatctct atgcaaccac gaaggtctgc tccgagcatc tggtccatgc ctacaccagc
     3301 atgtacaact tggacaccgt catcatccgg ccgggcggcg ttttcggacg cggtttctat
     3361 gtcggtgggt ccaccgtggg catgatcatg cgcgacctgg cattggcgat gatcaaagga
     3421 gagccgatca ctatcgaccc gaaaacctac ggtcccaacg agtatgtcta cggcaa
//
LOCUS       DATLCH010000213         3614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5788535, whole genome shotgun sequence.
ACCESSION   DATLCH010000213 DATLCH010000000
VERSION     DATLCH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5788535"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VMZ02_06290"
     CDS             <1..768
                     /locus_tag="VMZ02_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR71575.1"
                     /translation="KPFSRKVEFQDVNFAYAETPVLKDINLTVEAGQMVALVGMSGVG
                     KSTLVDLIPRFYDVKSGRILIDGVDVRDLSVKSLRAQIGIVTQHTFLFNDTIRNNIAY
                     GDPDRDMNSIIEAAKAAHAHEFIEAMPNGYNSEIGEMGLQLSGGQRQRLAIARALLKD
                     APILILDEATSSLDADSERSVQDALEALIRTRTTIVIAHRLSTIRKADRIIVMVNGSI
                     AEEGTHDELLARDREYSRLYSLQLLDNRQTNEAELVH"
     gene            785..2077
                     /locus_tag="VMZ02_06295"
     CDS             785..2077
                     /locus_tag="VMZ02_06295"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HUR71576.1"
                     /translation="MWFVGYNLLLTFVFLLTLPFTPILRLLGKQFSTGLWQRYGFFRQ
                     AELRSVAGSRPVWIHASSVGEVRSVAPLLRELQARHPQRRILLSTFTSTGNEIAKTFA
                     GVHAVIFLPADFLWSVRRALTQWNPSLMIFVETEIWPNLLAECFRRGIPTVMVSGRLS
                     ARSYPRYARFRGFFTQVVRRFNAIGMQSREDAARIIQLGADAVRVSVVGSLKLSSAKP
                     ESTRFTQVPRHGKKLLIAGSTHAGEEEILLSSFALLRSKFPELSMVIAPRHPQRFDEV
                     ELLVRGSGFSYCRRSQTSDGELFSRDILLLDSLGELAELFACADVVFVGGSLAQIGGH
                     NILEPARYGKAILFGPHMSNLKELAEEFKTNRAALEIVDGDDLSAALAILLSDEAKRI
                     AMGQRALAASRSGDAAITDNYALAARYLSDSPAVAHGD"
     gene            2104..3195
                     /gene="lpxK"
                     /locus_tag="VMZ02_06300"
     CDS             2104..3195
                     /gene="lpxK"
                     /locus_tag="VMZ02_06300"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HUR71577.1"
                     /translation="MPSSVVRGVWERSGFRGKLLAMLLWPVSLGYAGIVHLRNLFFAR
                     SWINSVRLPRPVVSVGNLTVGGTGKTPTCLWLAGELRRHGLKVAILSRGYRRKETLPV
                     ILQPSKLPDDIALNEDNLAKAGDEPLMMARLYEQTVGVGADRVQSARELLRRENVDAF
                     VLDDGFQHRRVQRDVDLVLLGSDCSGRMLPAGPFREPRRNLKRGHYWLVTGAEAEWRR
                     HLPPDSSSAVFSGSLQPLDLITFDANRWKRYPLSILHHSKILTVTGIANPQKLYQAIH
                     DWEGQIVHTLEFADHHPYSTRDWQEISRMARLIDLIITTEKDILKLIRYPFARDKLMA
                     LRVGMNVAGGDQLVAAVVERIRAACNSGT"
     gene            3192..>3614
                     /locus_tag="VMZ02_06305"
     CDS             3192..>3614
                     /locus_tag="VMZ02_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HUR71578.1"
                     /translation="MIAANLQPAVFLDRDGTLIRDVGYLTRIEQIEILAGVPAAIRAL
                     RNAGFATVVVTNQSAVARGWLTETALNHIHRLVQDLLAQEGAELDAIYYCPHHPSAGV
                     GEYRVVCDCRKPKPGMINRAVHDLALAPMRSYVLGDQNV"
BASE COUNT          751 a          946 c         1046 g          871 t
ORIGIN      
        1 aagccttttt cgcgcaaggt cgagtttcag gatgtcaact tcgcctacgc ggaaaccccg
       61 gtcctgaagg acattaatct cactgtcgag gcaggccaaa tggttgcctt ggttgggatg
      121 agtggcgtcg gcaaaagcac cttggtcgat ttgatcccac gattctatga cgtcaaatcc
      181 ggtcgaatac taatagacgg cgtcgatgtt cgcgatctgt cggtgaaatc actgcgtgct
      241 cagatcggta tcgtgactca gcatacgttt ctatttaacg atacgatccg caacaatatc
      301 gcatatggcg atcccgaccg tgacatgaac tccatcattg aagcggccaa agccgctcat
      361 gcgcatgaat tcatcgaggc gatgcccaat gggtacaatt cggagatagg tgaaatgggg
      421 ctgcagcttt ccgggggcca gcgccaacgg ttggcgatcg cgcgggcgct gctgaaagac
      481 gcgccgattc taatccttga cgaagcgact tcgtcgttgg atgccgattc cgaacgttcg
      541 gtgcaagatg ccttggaagc gctgatacgc acgcgcacca ccatcgttat cgcgcatcga
      601 ctgtcgacga tccgcaaagc ggaccgcatt attgtcatgg tcaacggctc catcgccgaa
      661 gaaggcaccc atgatgaact gcttgcccgc gatcgcgaat atagccggct ctactcgctt
      721 caattgctgg ataatcgtca aaccaacgaa gcggagttgg ttcactgaag gcgaccgccg
      781 cgccatgtgg tttgttggct ataacttgct gctcaccttc gtgttcttac ttaccttgcc
      841 gttcacacca attctgcggt tgttaggcaa acaatttagc actgggctgt ggcagcgcta
      901 tggctttttt cgacaagcag aactgcgctc cgtggccggc tcgaggccgg tttggatcca
      961 cgcatcgtcg gtcggcgaag tccgttcagt ggccccgtta cttcgggaat tgcaagcacg
     1021 ccatccgcag cggcgaattt tactttcgac attcacttca accggcaacg agatcgccaa
     1081 gacattcgcc ggagtgcacg ctgtcatttt cttgccggcg gattttttat ggagcgtgcg
     1141 gcgcgcctta acccagtgga atccatcgtt aatgatcttt gtcgagacag agatatggcc
     1201 taacttgttg gccgaatgtt ttcgccgcgg cattccgacg gtaatggtca gtggtcgctt
     1261 atcggcacgt tcctatccac gctacgccag attccgcggg ttttttaccc aagtcgtgcg
     1321 tcgcttcaac gccatcggta tgcagtcccg ggaagacgcc gcacgaatca tccagctggg
     1381 cgccgacgca gttcgggtat cggtggtcgg aagtctgaaa ttatcttctg caaagccgga
     1441 atcgacgcgt tttacgcaag tgcctcgcca tggcaaaaag ttgctgatcg ctggcagtac
     1501 gcatgcgggt gaagaagaga tactgctgtc gagtttcgcc ctgctccggt cgaagtttcc
     1561 cgagctatcc atggttattg caccgcgtca tccgcaacga tttgatgagg tggagctgct
     1621 cgtgcgcggc agtggcttca gctattgtcg aaggagccag acctccgatg gcgaactctt
     1681 ttctcgggat attttgctgc tggattcttt gggggaactc gccgagttat ttgcttgtgc
     1741 cgatgttgtt tttgtcggcg gcagtttggc ccagatcggc ggtcacaata ttctcgagcc
     1801 ggctcgttat ggtaaagcga ttcttttcgg accgcacatg agcaacttga aagagctcgc
     1861 cgaggaattt aaaaccaacc gcgccgccct cgagattgtt gacggcgacg atttgtccgc
     1921 tgcgctcgcc atattgttat cggacgaagc gaaacggatc gccatgggac agcgtgcgct
     1981 tgctgcgtca cgaagcggag atgcggcgat cacggataat tacgcgctcg cggcccgcta
     2041 tttatccgat tcgccggccg tagcccacgg tgattaggat tgaggagatt gttttagcgt
     2101 atcatgccta gttcggtggt gcggggggtg tgggagcgca gcggattccg cggcaagttg
     2161 ctcgcaatgc tactatggcc ggtgtcgttg gggtacgccg ggatcgttca tctgcgcaat
     2221 ctgtttttcg ctcgatcttg gatcaattcg gttcgactgc ccagaccggt tgtcagcgtc
     2281 ggtaatctaa cggttggagg gacgggaaag acgcctactt gcctttggct ggccggtgag
     2341 ttgcgccgtc acggcctgaa agtcgcaatc ttgagccgcg gataccggcg caaggaaacg
     2401 ctaccggtta ttttgcagcc gagcaaattg cctgacgata tcgctctcaa cgaagacaac
     2461 ttggcaaagg ccggcgatga accgctgatg atggcgcgtt tgtatgagca gacggtaggt
     2521 gttggagcgg atcgtgtgca atccgcgcgc gaattgctgc gccgggaaaa tgtcgacgcg
     2581 tttgttcttg atgatggatt tcagcaccgg cgagtccaac gcgatgtcga tctcgttctt
     2641 ctcggcagcg attgttcggg gcgaatgtta ccggcgggcc cgttccgtga accgcgacgc
     2701 aatttaaagc gaggtcatta ttggcttgtc accggagcgg aagccgagtg gcgccgtcat
     2761 ttgccgcccg atagttccag cgcggttttt tccggttccc tgcagccctt ggatctgatc
     2821 acttttgacg cgaaccgttg gaagcggtat ccgctgagta ttttgcatca cagcaaaatt
     2881 ctgacggtca ctggcatcgc caatccgcaa aaactctacc aggctatcca cgactgggaa
     2941 ggccagattg ttcatacgtt ggaattcgcc gatcatcatc cttacagtac gagagattgg
     3001 caagagatca gcaggatggc acgcctgatc gatttgatca ttacgaccga gaaagatatt
     3061 ctcaaattga ttcgttatcc gtttgcccgg gataaactga tggcactgag agtcgggatg
     3121 aatgtagccg ggggagacca actagtggca gccgtcgtgg agcgaattcg cgcagcgtgt
     3181 aatagtggta catgattgcg gctaatctcc aacccgccgt attcctggat cgcgacggca
     3241 cattgattcg tgacgtcggt tacctcacgc gaatcgaaca gatagaaata ttggctggcg
     3301 tgccggcggc aatccgtgcc ttgcgcaatg ccgggtttgc aaccgtggtc gtaaccaatc
     3361 agtcggccgt cgcgcgcggt tggcttaccg aaacagcatt gaaccatatt catcgactcg
     3421 tccaggactt gctggcccag gaaggtgcgg agttggacgc gatttattat tgcccgcatc
     3481 atccgagcgc aggcgtcggg gagtatcggg tggtgtgcga ttgccgcaag ccgaaacccg
     3541 gcatgatcaa tcgtgcggtc catgatctgg cattggcacc gatgcgatcc tatgtgctcg
     3601 gcgatcagaa tgtc
//
LOCUS       DATLCH010000214         6403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1322781, whole genome shotgun sequence.
ACCESSION   DATLCH010000214 DATLCH010000000
VERSION     DATLCH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322781"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1260)
                     /locus_tag="VMZ02_06310"
     CDS             complement(238..1260)
                     /locus_tag="VMZ02_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71579.1"
                     /translation="MPNNYVDADGHVMEDSEDILSFVKAPYNNRGTRNWIPSLDHFHT
                     PADGTPRTPGTFDPSTGPEQWLQFLEKTGTEYTVLYPTAGLAYGNVAYPTWALAYAQA
                     YNDYIHARYLKRSPKFQAVSLIPMQNVADAVSELRRSVKELGFMGAMIPSNGLTRHVS
                     HQEYWPIYEEAEKLNCVMAVHGGSYINLGFNSYTVFPATRALGMPFPLAIAMTGMMVD
                     GVFDRFPKLRVGFMEGGTAWIPLVIDRLERELEYGGLKLKRHPSDYFNDDRIFVGCEG
                     NEKALAYAIERVGPKPFMFASDFPHEISMSNCMEEIDEVLERDDLKKEHKTAILGDNA
                     RRFYGR"
     gene            complement(1708..2688)
                     /locus_tag="VMZ02_06315"
     CDS             complement(1708..2688)
                     /locus_tag="VMZ02_06315"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71580.1"
                     /translation="MTQRRPWLTNAAASALVALLLPAPIGAAEKNKVTLGYSSTGPTA
                     VGLWMAQEIGAFNKYAIEGNFVFISSSPVMVPALIGGDVQCAIAGANAVIAAALGGAP
                     IVSIASLANRPYLRLWAHPEFTRVEELRGKTLGVSRFGSTTDNLTRILLRRVGMENAV
                     NIRQLGGTLEVGLAFRHRQIDGAVLATLRTDAPHRILIELAESGIQYSMGQLVVAREF
                     QRRSPDTLEKIMRAYIEGVAALRDPSQKSRVTKAIAYFSRLKEAKAIDEIYNDAAKYV
                     DRIPRVEAEPVAATLEMMGKKGLSISSFADNSIIDKLIREGFIEQVYKSR"
     gene            complement(2903..3904)
                     /locus_tag="VMZ02_06320"
     CDS             complement(2903..3904)
                     /locus_tag="VMZ02_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71581.1"
                     /translation="MLGREVLRIVVAAFLLHGGIIYAAESTGLRKIRSAITSISGSMS
                     PAWAAHESGIFAKHGLQVEVVAMPSGLQGITTLAAREVDFVHIAGGTTAGAAIGGLDV
                     KILATLVGTLVLNLVVRPEIEKPEQLRGKTIGISRYGTSLHTGARIALRHFGLGDGKD
                     VAIAEIGAGEWIVGAMQGGRIQGGVFGYPQTSRAIKLGNRLMLHLPTLNIAYAANGVS
                     TRGEIIRNDPDLVRRYLAAVIETSALMKKDRAFSFNVLRKYLRTSDTDLLSETYDVQI
                     AKYLLKVPLPTVENVRSVVEEIAERNAKAKDQDVKLFFDDRFVRQLETSGFIDGVYR"
     gene            complement(3991..4401)
                     /locus_tag="VMZ02_06325"
     CDS             complement(3991..4401)
                     /locus_tag="VMZ02_06325"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HUR71582.1"
                     /translation="MATKFTGGCMCGKVRYECTAEPIFMGNCHCRDCQKATGTAYEAA
                     IGIPAATLKVTGDVKFYDNKAESGNIISRGFCPECGSRLLGKASAVPDLAMITAGSLD
                     DPKQYVPQMDIFTSSAQPWDHMNPALPKFPKMPM"
     gene            complement(4437..5099)
                     /locus_tag="VMZ02_06330"
     CDS             complement(4437..5099)
                     /locus_tag="VMZ02_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR71583.1"
                     /translation="MAIELYWGSGSPFAWRVMLTLEVKGLAYESKLLEFSKGDHKTPA
                     YLQLNPRGKVPTLKDGDFVIYESLAIMSYLDRKYPAKPLFGTTAEETGLIWQTISECE
                     SYFTSGGDKVVRPLFFGKGLDKVDEIQEAAEIIREELKTLDVRLATRNWLVGETISAA
                     DIAIFPLIQLILRAAGKDAAAPFNLRWLPLAQFYPHVAKWCERIEALPNYQRTFPPHW
                     KA"
     gene            complement(5421..6140)
                     /locus_tag="VMZ02_06335"
     CDS             complement(5421..6140)
                     /locus_tag="VMZ02_06335"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR71584.1"
                     /translation="MRYELYYQPSIQGRGEFVRLALEDAGADYVDVARDPEFGRPGIV
                     KILEDPSVEHPSFAPPFLKAGKLMIGQTANILQFLGPRLNLVPDDQARRLWVHQLQLT
                     VTDWLAEVGNTHHPIANVLYYEEQRDEAKRRTSYFTSLRIPKFMGYYEKILQKNGKAA
                     PYLLGKQISYADLSLFQMVAGLRYAFPRAMAKVEPSCPRVVAVHERVAARPRIKKYLA
                     SPRRLAFNTNGIFRHYPELDE"
BASE COUNT         1369 a         1698 c         1816 g         1520 t
ORIGIN      
        1 gccagactgt gtgaaaactc agcgtctctc tgatctgtca tctcgaacgg cgtgagagat
       61 cttgcatttt gtacacatag aaagacaaga tttctccgct tcgcgtcgaa atgacaagta
      121 ccacatagtc tggcgcagcg aggggtatcc gtccttctgc cgatctcaaa gacacgattt
      181 ctcggcttcg cctgagcatg acaactgcga gaaattgcgc ctaccgacgt ccgattctca
      241 tcggccgtag aaacgccggg cgttatcgcc aaggatcgcc gtcttgtgtt cttttttcag
      301 atcgtcgcgt tccaacactt cgtcgatctc ttccatgcag ttgctcatgg aaatttcatg
      361 gggaaaatcc gaggcgaaca tgaacggctt cgggccgacg cgctcgatgg cgtaggcgag
      421 tgccttctcg ttgccctcgc agccgacgaa gatacggtcg tcgttgaaat aatcgctggg
      481 atggcgtttg agtttcaagc cgccgtattc cagctcgcgt tcgagccggt cgatgaccag
      541 cgggatccat gccgtgccgc cttccatgaa gccgacgcgc aacttgggga aacggtcgaa
      601 tacgccgtcg accatcatgc ccgtcatcgc gatggcaagt ggaaagggca tgcccaatgc
      661 ccgcgtggcg gggaaaaccg tgtagctgtt aaagcccaaa ttgatgtagc tgccgccgtg
      721 caccgccatg acgcaattta atttttcggc ctcttcgtaa atcggccaat actcctggtg
      781 actgacatga cgggtcaggc cattggacgg aatcatcgcg cccatgaagc ccagctcttt
      841 gaccgagcgg cgcagctcgc tgacggcgtc ggcgacgttc tgcatgggaa tcagcgacac
      901 ggcttgaaat ttcgggctgc gcttcagata gcgggcgtga atgtaatcgt tgtacgcctg
      961 ggcgtatgcc aacgcccagg tcggatacgc gacattgccg taggctagac ccgctgtcgg
     1021 atagagtacc gtgtattcgg tgccggtctt ctccaaaaat tgcagccatt gctccggccc
     1081 agtggaggga tcaaaggttc cgggtgtgcg cggcgtgccg tccgccggtg tatgaaaatg
     1141 atccaagctc ggaatccaat ttcgcgtgcc acggttgttg tagggcgctt tgacgaaact
     1201 gagaatatcc tcggaatctt ccataacatg gccatcggcg tcgacgtagt tattgggcat
     1261 aattcctcct ctgtgtaagc cacttgcaat cgttgtaggg aatcgactcg gacaatctga
     1321 atcgatcatt atgtttatat ggcggctgag atcgagaggt caagtggggt tgtcattgtt
     1381 tcgccaatcc accgttagac gttccgagcg cgactcatta cgtcgagtac actgccgaaa
     1441 cgacgctgct cgatgtcgcc aagaatgaag ccgtctggac cgcgcgacag tggctaaaga
     1501 gaaggaaagc ggggggaaac ccggtcaaga gcaccgtcga tgctgtggtg aaagcgtttt
     1561 cacagaacaa ttttttgcgc cggactcagt gaggtaaaaa cgattccata caaagacgcg
     1621 gcagtcgata aaaaaagaat ttggaattat tttcattgtc attgcgtctc tgcctgaaat
     1681 gattcggttg gtctctttct ctaacggtca tcggcttttg tagacctgtt cgatgaatcc
     1741 ttcgcggata agtttgtcga tgatcgagtt gtcggcgaac gaggagatgg ataaaccttt
     1801 cttgcccatc atttccagag tcgcggccac tggttcggct tcgacgcgtg gaatgcggtc
     1861 aacgtatttg gcggcgtcat tgtagatctc atcgatcgct ttggcttcct tgagacggct
     1921 gaaataggcg atggctttgg tcacccggct tttctggctt ggatcgcgca gcgcggcaac
     1981 tccttcgatg taagcgcgca tgattttctc caatgtgtcg ggtgaacggc gctggaattc
     2041 gcgcgctacc actagttggc ccatggaata ctgaatgccg gactcggcca gttcgatcaa
     2101 tattcgatga ggcgcgtcgg tgcgcagcgt tgccagaaca gcgccatcga tttgccgatg
     2161 gcgaaaagct aatccaactt ccaaggtgcc acccagctgc cggatgttga ccgcgttttc
     2221 cataccgact cggcgcagca gaatccgcgt gagattgtcg gtcgtcgaac cgaagcgcga
     2281 cactccaagt gtcttgcccc gaagttcttc gacccgggta aattcggggt gcgcccacaa
     2341 acgcaggtac ggacggttag cgagactagc gattgaaaca atcggcgcgc cacctagcgc
     2401 ggcggcgatc acggcatttg ctccggcaat ggcgcactgc acgtccccgc cgatcaacgc
     2461 cggtaccatt accggactcg acgagatgaa aacgaagttg ccctcgatgg cgtatttgtt
     2521 gaaggcgccg atctcttggg ccatccacag gccgacggca gtggggccgg tactggaata
     2581 accgagagtg actttatttt tttccgccgc tccaatcggc gccggcaaca acagcgccac
     2641 gagtgcggac gctgcggcat ttgttagcca tggccgtctt tgcgtcatgc taatgccctc
     2701 tccggaatgg ttgaaataac ttgcattcag gcgaagagaa gattgtcagg gcgcaggccg
     2761 gcatgcatcc tagtttgcgc tgcgaagccg atgaaaccct ggattccggc ctccgccgga
     2821 atgacgaaag gaaggggtcg acttcccgtc gtcgaattct gaactgctca taaaagccaa
     2881 gtgtatatca gctcaaatcg cttcagcggt agacgccgtc gataaagcca ctggtttcca
     2941 attgccggac aaagcggtcg tcgaagaata gcttgacatc ttggtctttg gctttggcat
     3001 tgcgctcggc gatctcctca actaccgacc tgacgttttc gaccgtcggt agcgggactt
     3061 taagcagata cttggcgatc tgcacgtcat aggtttcgct caatagatcg gtgtcgctgg
     3121 tgcgcaaata tttgcgcaag acattgaagc taaaggcgcg atctttcttc atcagcgcgc
     3181 tggtctcgat gacagcggca agatagcggc ggaccaaatc gggatcgttg cggataattt
     3241 caccgcgagt cgatacaccg ttggcggcat aagctatatt gagcgtgggc agatgtagca
     3301 tcaggcggtt gccgagcttg atggcacgcg acgtctgcgg atagccgaac acgccgcctt
     3361 ggatgcggcc gccctgcatg gcgccgacga tccactcgcc ggcgccgatc tcggcaatcg
     3421 cgacgtcttt gccgtctcct aaaccgaagt ggcgcagggc gatgcgtgcg ccggtatgca
     3481 gcgaggtgcc gtagcggctg atgccgatgg ttttgccgcg cagttgttct ggcttttcga
     3541 tttccgggcg caccaccaga ttgaggacca gagtaccgac cagcgtagca aggatcttta
     3601 cgtcaaggcc gccaatggct gcgccggcgg tcgtgccgcc ggcgatatgg acgaagtcga
     3661 cttcgcgcgc ggccagggtg gtgatacctt gcaagccgct cggcatggcg acgacttcca
     3721 cctgaagtcc gtgtttggcg aaaattcccg attcatgcgc ggcccaggcc ggcgacatgc
     3781 tgccggagat gctggttatg gcggagcgga tcttgcgcag gccggttgat tcggcggcat
     3841 agatgattcc gccgtgaagc aaaaacgccg caacgacaat tcgcaaaact tcgcgcccga
     3901 gcatcgctca cctcgcgttc gagttcgtgg accagtgccg ttaggcaccg ctggtcccgt
     3961 ggcgcatctt ataactcaga ttaaaacaat ttacatcggc attttgggaa acttcggcaa
     4021 ggcgggattc atatggtccc agggctgggc gctcgacgtg aaaatatcca tctgcggtac
     4081 atattgcttg ggatcgtcaa gactgccggc ggtgatcatc gcgagatcag gcactgcgct
     4141 ggctttaccc aataggcgcg aaccgcattc ggggcagaag ccgcgactga tgatgttgcc
     4201 gctctccgct ttgttatcgt aaaattttac atcgccggtg actttgagag tcgccgccgg
     4261 gatgccgatg gcggcttcat aggcggtgcc ggtggctttt tggcagtcgc gacagtggca
     4321 gttgcccatg aagatcggct ctgcagtgca ttcgtaacgg actttgccgc acatacaccc
     4381 gccggtaaat ttagtcgcca tggtttgctc cttttgaacg tgaatcgtct gtacaatcac
     4441 gctttccagt gcggcgggaa tgttctctga taattcggca aggcttcaat ccgttcgcac
     4501 catttagcaa cgtgcggata gaactgcgcc agcggcagcc agcgcagatt aaacggcgct
     4561 gcggcgtctt tgccggccgc gcgtagaata agttggatca acggaaatat tgcaatatcc
     4621 gcagcgctga tggtttcgcc gaccaaccag ttgcgagttg ccagccggac gtcgagagtt
     4681 tttaattctt cccggatgat ttcggccgct tcctgaatct cgtcgacctt gtccaagcct
     4741 ttgccgaaaa acagcggccg aaccactttg tctccgccgc tggtgaaata cgactcgcat
     4801 tccgaaatag tctgccagat cagaccggtt tcctcggcag tggtgccaaa cagcggctta
     4861 gccggatatt tgcgatcgag gtagctcatg attgccagcg attcgtaaat aacgaagtcg
     4921 ccgtctttga gtgtcggcac cttgccgcgc ggattgagtt gcaggtaggc cggcgttttg
     4981 tgatcgcctt tggaaaactc caaaagcttc gactcgtagg ccaagccttt cacttcaagt
     5041 gttaacatga cgcgccaggc gaatggactg ccgctgcccc aatatagttc gatagccatg
     5101 atcaactgcc ctccctctat taaggtagtt aagcgcgctg attctcgaat aacaactacg
     5161 tgaaaggatt gaagtctctt tcctcgtcgg caggttggtc acgcttaatt attatgtcgt
     5221 ttcgatcgcc agactgtgtg gtaactgtca tttcgatgcg aagcggagaa atcttgtgtt
     5281 tctatgtact caaaatgcaa gatctctcac gccgttcgag atgacagatc agagagacgc
     5341 tgactttttc acacactctg ccgcagcgat aaatccaaga tctctggttc gagatgacac
     5401 tcgattccgg atccgagacg ctactcgtcg agttccggat agtggcgaaa aatgccgttg
     5461 gtgttgaacg ccagacggcg cggcgatgcg agatattttt ttatccgcgg tcgcgcagcg
     5521 acgcgctcgt gtacggcgac aacacgaggg caactgggtt ccactttcgc cattgcgcgc
     5581 gggaacgcat agcgcagccc tgcgaccatt tgaaacagcg acagatcggc gtaagaaatt
     5641 tgtttgccga gcaggtaagg agcggctttg ccgttttttt gtagaatttt ctcgtagtaa
     5701 cccatgaact tgggaatgcg tagcgacgtg aagtatgacg ttcgcctttt ggcttcgtct
     5761 cgctgttctt cgtaataaag tacgttggcg atcggatgat gcgtattgcc gacctcggca
     5821 agccaatctg tgaccgtcaa ctgcagttga tgcacccaaa gccgcctcgc ctggtcgtcg
     5881 ggcactagat tcaggcgagg cccgagaaat tgcagtatgt tggcggtttg gccgatcatg
     5941 agtttccccg cttttaaaaa aggcggcgcg aaggacggat gctccacgct gggatcttcg
     6001 agtattttca cgatgccggg gcggccgaat tcgggatcgc gagcgacatc gacataatcg
     6061 gcgccggcgt cttccaatgc caagcggacg aattcaccac ggccttggat ggatggttgg
     6121 tagtagagtt cgtagcgcat ggttttctcc tttgtcgagg attgatgatc gaggatcgag
     6181 gatggcgaca gattaaaact tcctcctggg tctatcgatc ctctatattc tatcctccat
     6241 cctcgaccaa tttctcagcg tggcgttagt ttgtcgatga agccgctcgt ttccaacgtt
     6301 ttaagataac tgttatcgaa gataccttta acgtcgactt tgggcagagg cggcccctgg
     6361 gtttgcatgg cctcgatggt tgaccgtgcc gagacttcgt cga
//
LOCUS       DATLCH010000215         5778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2816950, whole genome shotgun sequence.
ACCESSION   DATLCH010000215 DATLCH010000000
VERSION     DATLCH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816950"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VMZ02_06340"
     CDS             complement(<1..217)
                     /locus_tag="VMZ02_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71585.1"
                     /translation="MVPLDILRRERTLDLWNFVAQYAGRMHQERIKKVVRKGKLTREI
                     TVLMQDEDIDLLVLRQRRRRLLPDLTTW"
     gene            complement(189..422)
                     /locus_tag="VMZ02_06345"
     CDS             complement(189..422)
                     /locus_tag="VMZ02_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUR71586.1"
                     /translation="MQKIRKILVPIDFSQEAGGALRDAAVLGRETGAQVVALHVIDEK
                     AERNVLLSAIAPVDGLPFFFGQLRNGAAGHSAT"
     gene            complement(476..1471)
                     /locus_tag="VMZ02_06350"
     CDS             complement(476..1471)
                     /locus_tag="VMZ02_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71587.1"
                     /translation="MKRKLFARLILLMVLGFALPCLAQEKLEKIYVGVAGLSGALAHA
                     FIPKDTGLYEKYGLDVDLVFFQGGTQGVQSLIAGGVQFTVSSGSELVAARVAGSDVTI
                     VAGYMNTVPYSIVADKRVTRFDDLRGGKAAVSRFGTTSDLAVRYAFEQNGLIPGKDIT
                     LVQLGDQGARFAALTGGTVQATVISPPFDLTAKKLGYRILADMADLGLHYQHEALATT
                     ERMLTARPETVRRFLKAFIAGIHVWMTDEKKTKEILARRLKIKDNEILDETYSAYKKL
                     TEKKPYPTLKGLDFQIADLAVRNPKAKNAKADAFVNIGLLKEIDKSGFIDGLYKR"
     gene            complement(1520..2398)
                     /locus_tag="VMZ02_06355"
     CDS             complement(1520..2398)
                     /locus_tag="VMZ02_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71588.1"
                     /translation="MDKEDVSSAIRKVHSLTSDRQAETANAHKVWRMLLGCAVIALVL
                     GGSNASAQYSRDSWFFSAAEIGAAYQYQENYGGRMRNPLPAFSCMTGQAEFVATLRRV
                     PVRVACPFVNHVMRHLKEMLDVGAARYLFPLDADHAHLAVPAAVWEEKYSKLSSTQLL
                     VALLREPQLVALYHTAEHLEPAAKEKRSDDAAVRRWREQRNVLGYFDGRPLQILPPHP
                     EGFGVGLPDGLMSYGGFNFLASPHGELMILQGQRAIAFDVSLEMEEPEPSGFGRSLAR
                     VAPWAPGPAGRRTVRD"
     gene            complement(2569..3336)
                     /locus_tag="VMZ02_06360"
     CDS             complement(2569..3336)
                     /locus_tag="VMZ02_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR71589.1"
                     /translation="METTNAVTELNPRIFQIRGLGGSSHCYVIQGDYMNVMIDSGSDQ
                     NFPVLERGLRHIGMKVRDINLVINSHEHCDHIGGNRYFQESAMIAAYRLAASKMTTGD
                     YYVTMYKGNDLNELPMRVHLWLENMTRIDLGNYTLRVFHTPGHTSGCISILETTSGLL
                     FSADSVFAGGTLSYIAESGSIGDYLDSLARLRSFNLKALYPGHGRVSTTPIEDIDRAM
                     VNAQMLLETNNHETVDIFYQNAHENPEANERETTVSN"
     gene            complement(3311..3673)
                     /locus_tag="VMZ02_06365"
     CDS             complement(3311..3673)
                     /locus_tag="VMZ02_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HUR71590.1"
                     /translation="MNYRITEVAGWVIVTPSGKAENNEPLRVRHLFRRWLTRQGVRVI
                     VNLKQLEQFGVWEVGVLTSFKRQVDQRVGVLRLCNLHPALQGYFNHDRFSECFEIYND
                     LEAAMQGDNRSGNHECGH"
     gene            complement(3679..3876)
                     /locus_tag="VMZ02_06370"
     CDS             complement(3679..3876)
                     /locus_tag="VMZ02_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71591.1"
                     /translation="MLKTGKTATERILENIIFLADVFGEQLEKDIPVWPILEMARCFE
                     LAPRTIAHTSQARRKDWVLWN"
     gene            complement(4200..4787)
                     /locus_tag="VMZ02_06375"
     CDS             complement(4200..4787)
                     /locus_tag="VMZ02_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR71592.1"
                     /translation="MKKVLVIDDHEPSRRQLVASLRSCGYQVAAEGSSGKVALALSQA
                     SGSEVIIMAVGLPDLDGIDAAREIMQTRPLPIVLITSHADASTVMRATHAGVMSYLVK
                     PLRVEELAPAIELAVSRFRESVSLRDEVSTLKLNLQERKLIERAKGLLMEQRHLSEER
                     AYALMKKASMNMRKPMADIAQAILLAGGLLTESNP"
     gene            complement(4784..5530)
                     /locus_tag="VMZ02_06380"
     CDS             complement(4784..5530)
                     /locus_tag="VMZ02_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HUR71593.1"
                     /translation="MGEVVVFQHDPFEDLGFFAEVLEKQQTAFRIVRLFHDEMPAENW
                     DEVKALIILGGPMNVDDEASFPFLRWEKRIIRAAIDDAVPVLGVCLGAQLIATTLGSA
                     VFHGSVKEIGWSPISITPHGQVDSLLGYLPEIATVFQWHGDGFELPSGAILLASSQHF
                     RTQAFRLGKTIYGLQFHLEVTPRMIERWIEERSKDLALAPYVQPDKIMADTHNYAPAL
                     KYYGERFLSEFVRRVAHSKNRKGDGGVLPV"
BASE COUNT         1320 a         1630 c         1506 g         1322 t
ORIGIN      
        1 tccaagttgt caggtcagga agcagccgcc gacgacgttg tctaagcact agtaaatcga
       61 tgtcctcgtc ttgcataagg acggtgattt cccgagtcaa tttgcccttt ctgactactt
      121 tcttgatgcg ttcctgatgc atccgcccgg catattgcgc gacaaagttc cacagatcga
      181 gagttcgctc acgtcgcaga atgtccagcg gcaccattgc gtaactgtcc aaagaaaaac
      241 ggcaaaccat cgaccggtgc gatagccgac aacaaaacat ttcgttcggc tttttcatcg
      301 atgacgtgta gcgcgaccac ctgcgctccg gtttcccgtc caagcacggc ggcatcacgc
      361 agcgcgccgc cagcctcctg ggaaaagtca atcggcacga gaatctttct aatcttttgc
      421 attttaatct cctcccgcaa actcggtcac gatgctttgt ttgactcgag catggttatc
      481 ttttgtacag accgtcaata aagccgctct tgtcgatctc cttcagcagg ccaatattaa
      541 cgaaggcatc ggccttggcg tttttcgcct tcggatttct gaccgcaaga tcggcgatct
      601 gaaagtcgag accctttagc gtcggataag gttttttttc cgttaatttt ttgtacgcgg
      661 aataggtttc gtcgagaatt tcgttatctt tgattttcaa ccgcctggcc aaaatctctt
      721 tggtcttctt ctcgtcggtc atccacacat gaatgcccgc gatgaacgct ttgagaaacc
      781 gccgcacggt ttccggtcgc gctgttagca tgcgttcggt ggtggccaag gcttcgtgct
      841 gatagtgcaa accgagatcc gccatgtcgg cgagaatacg atagcccaat ttttttgcgg
      901 tgagatcgaa cggcggcgag atcaccgtcg cttgcacggt gccgccggtg agcgcggcga
      961 agcgcgcgcc ctgatctccc aattgcacta aagtaatgtc cttgccggga atcaacccgt
     1021 tttgttcgaa ggcgtagcgc actgcgagat cagaggtggt gccaaaccgg ctaaccgcgg
     1081 ctttgcctcc tcttaaatca tcgaaacggg tgacccgttt atcggcgacg atgctatagg
     1141 gcaccgtatt catgtaaccg gcgacgatgg tgacgtccga accggcgacg cgcgcggcaa
     1201 cgagttcgga tcccgacgag acagtgaact gcacaccccc ggcgatgagc gattgcactc
     1261 cttgggtgcc gccctgaaaa aatactaaat cgacatcgag accatacttt tcatacaagc
     1321 cggtgtcctt ggggatgaat gcatgcgcca atgcgccgct caaaccggcg acgccgacat
     1381 aaatcttttc cagtttctcc tgcgctaagc atggcaacgc gaagcctaag accatcaata
     1441 aaattagtcg ggcgaacaat tttcgtttca tgcggaccct ttcaattttc gctattgtcg
     1501 gacgcctttt gcataagaat taatctctta ctgttcgccg tccagccgga cccggcgccc
     1561 atggggcaac ccgcgctagc gaacggccga aaccactcgg ttctggctct tccatttcca
     1621 agctaacatc gaaggcgatc gcccgctggc cctgaaggat catcaattcg ccatgagggc
     1681 tggcgagaaa attgaacccg ccataggaca tcaatccatc aggcaagccg acaccgaaac
     1741 cttctggatg cggcggcaag atttgcaggg gtcggccgtc gaagtagccc aagacgttgc
     1801 gctgctcgcg ccagcgccgg actgccgcat cgtccgatcg cttttccttc gcagcgggtt
     1861 cgagatgctc cgccgtatga tagagggcga cgagttgggg ctcgcgcagg agcgcgacca
     1921 gcaattgcgt cgaggaaagc ttgctgtact tctcctccca aacggcagcc ggcaccgcca
     1981 ggtgggcatg atcggcgtcg agcggaaaca gatagcgcgc tgcgccgacg tcgagcattt
     2041 ctttcaagtg acgcataacg tgattgacaa acggacaagc cacgcgcacc ggtacgcggc
     2101 gaagcgtggc gacaaactct gcttggccag tcatacagct gaaagcgggc aacgggttac
     2161 gcattcggcc gccatagttt tcttgatatt gataagcggc gccaatctcg gctgcggaaa
     2221 aaaaccagga atcgcgtgaa tactgcgccg acgcattgct tccgccgaga accaaggcta
     2281 tgacagcgca gcccagcaac atgcgccaga ctttatgcgc atttgccgtc tcggcttggc
     2341 gatcgctggt caggctatga accttacgga ttgcactgga aacatcttct ttgtccatgg
     2401 cattcacctc cgtagtcgca acttacaaat aaaaatggcg ctcgattccc tgcgagaatc
     2461 tggcgccatt gccacagtca cggacttcag cttcgaagcc cgggaccgct cttgctgcgc
     2521 ccgcggcgcc cttacgccgc acgcgcgatc atacttacga gccgtttgtt aattgctgac
     2581 agttgtctcc cgttcgtttg cttccggatt ctcatgggca ttctgataaa agatgtcgac
     2641 cgtctcatga ttgttggttt ccagcagcat ctgggcattg accatcgcgc gatcgatgtc
     2701 ttctatcggc gtcgttgaaa ctcgcccgtg gccaggatag agcgctttca aattgaaact
     2761 gcgcaaccgc gccagcgagt cgagatagtc accgatgctg ccgctttcgg cgatgtacga
     2821 gagcgtgccg ccggcaaaca cgctgtcggc ggaaaagagc aatccgctgg tggtctcgag
     2881 gatcgaaatg caaccggagg tgtggccagg cgtatgaaac acccgtaatg tgtagttgcc
     2941 gagatctatg cgcgtcatat tttctagcca cagatgcacc cgcatgggca gctcgttgag
     3001 atcgttgccc ttgtacatgg tcacatagta atcgccggtg gtcattttcg aagcggccaa
     3061 acggtaagct gcgatcatcg ccgactcctg aaagtaacga ttgccgccga tgtgatcgca
     3121 atgctcatga gaattgatca ccaagttaat gtcgcgcact ttcatgccga tatggcgcag
     3181 tccgcgctcg agcaccggga aattctggtc cgaaccggag tcgatcatga cgttcatgta
     3241 atcgccttga atgacataac agtggctgct gccgccgagt cctcttatct ggaatattct
     3301 cggattgagc tcagtgaccg cattcgtggt ttccacttcg attatctcct tgcatcgccg
     3361 cttcgagatc gttgtagatc tcgaagcatt cggaaaatcg atcgtgattg aaatatccct
     3421 gcaacgccgg gtggagattg cagagccgca atacgccgac ccgctgatcg acctgacgtt
     3481 taaaagacgt tagtacaccc acctcccata ctccgaattg ctcgagttgc ttaaggttga
     3541 cgatcacgcg gacaccctgg cgcgtgagcc agcggcggaa taaatgacgc acccgcaacg
     3601 gctcattgtt ctcggccttg ccggaaggcg taacaataac ccagccggca acttccgtga
     3661 tgcgataatt caccgggctt aattccacag cacccagtct ttccgccgcg cttggctggt
     3721 atgagcgatg gtccgcgggg cgagttcaaa acaacgcgcc atctccagta tcggccacac
     3781 aggaatgtct ttctccagct gttcgccgaa cacgtcggcc aagaaaataa tgttttcgag
     3841 aattctctcg gttgcggttt tgccagtctt cagcattctc ttacctccaa aaaattaagg
     3901 cgctccaccg ggctacggtg agcgcctttg ctcaacccag gggcaacgtc gccctcagtc
     3961 acgtaacaac gcaaccgctg tgccacggga cggcgatcac aaaatcgcgt tatcatgacg
     4021 ttttaatatt tttgtaatat gagacgcctg ttcgatagtc acgaacgtgc ctaaacaacg
     4081 agcaggtagg gacacgggcg aaatagaatt gggagaatct ttgtcgacga acggaaaaat
     4141 gccgacacgc ggagcagttc aagtaacgtg gattactgag ttgaatttca cgtcattaat
     4201 tacggatttg attccgtaag cagcccaccg gcgagcagta tcgcctgcgc gatgtcggcc
     4261 atcggcttgc gcatgttcat gctggctttt ttcatcagcg cataggcgcg ctcttccgaa
     4321 agatggcgtt gctccatcag caagcctttt gcgcgttcga ttagtttgcg ttcttgaaga
     4381 ttgagcttga gcgtggagac ttcgtcgcgc agggaaacag attcacggaa gcggctgact
     4441 gcgagctcga tggcgggcgc gagttcttcg actcgcaggg gttttaccag ataactcatc
     4501 actcccgcat gagtcgcgcg catcaccgtt gaagcatcgg catgactggt aatcaacacg
     4561 atcggcaacg gacgggtttg catgatctct cgcgccgcgt cgatgccatc gagatccggc
     4621 aatcccaccg ccattatgat gacttcagat ccggaagcct gagaaagcgc caatgccact
     4681 tttccactcg agccctcggc ggcgacttga taaccgcagc tgcgtagact tgctacgagt
     4741 tggcgccgcg agggttcgtg atcgtcgatg accagtactt tcttcatacc ggcaataccc
     4801 caccatcccc ttttcgattt ttcgaatgag cgacgcgccg gacaaactcg gaaagaaatc
     4861 gctcgccata atacttcagt gccggcgcat aattatgcgt gtccgccatg attttgtccg
     4921 gttggacgta aggcgccagc gccaagtctt tggaccgctc ctcaatccat cgttcgatca
     4981 tgcgcggagt tacttcaaga tgaaactgca gcccatagat ggttttgccg aggcgaaacg
     5041 cctgggtgcg aaaatgctgc gatgaagcga gtaggattgc cccgctgggc agttcaaaac
     5101 cgtccccatg ccactgaaac acggtagcaa tttccggcag atagccgagc aatgaatcga
     5161 cctgaccgtg cggcgtaatg gaaattggac tccaacctat ctctttgacc gagccgtgaa
     5221 ataccgccga gcccagagtc gtggcaatca actgcgcgcc taagcatacg cccaaaaccg
     5281 gcacggcatc gtcaatagcc gctcgtataa tccgcttttc ccagcgcaaa aacgggaaac
     5341 tcgcctcatc atcgacgttc atcgggccgc ccagaatgat cagggctttg acttcgtccc
     5401 agttttccgc cggcatctca tcgtgaaata gacggacaat tcgaaaggca gtttgctgct
     5461 tttcgaggac ttcggcgaag aaacccagat cttcgaatgg gtcatgctga aaaacgacga
     5521 cctcacccat gggtccccca aaagttatta ctgggccagt gaaatttgca ctgaagctat
     5581 ttcacctcag tacaaattgt ccgcggttag atatcaaaat acagagcgaa ctcccaagga
     5641 tgggggcgca gccggatggc atcgacttca tgcttgcgtt tgtagtcgag ccaggtctcg
     5701 atcacgtcct tggtaaacac gtcgccggtg agcaagaagg catggtcctt ctccagagcg
     5761 tcgagcgcct gatccaac
//
LOCUS       DATLCH010000216         9265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4290811, whole genome shotgun sequence.
ACCESSION   DATLCH010000216 DATLCH010000000
VERSION     DATLCH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4290811"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1263)
                     /locus_tag="VMZ02_06385"
     CDS             complement(316..1263)
                     /locus_tag="VMZ02_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUR71594.1"
                     /translation="MEYRQMGNTGVRVSVVGLGTNRFGSADMPQSEVNKVIDVALDAG
                     VNFLDTANVYNEGRSEETLGVALKGRMDNVVMATKFNFPRKTSANSWGASRYQMMQSI
                     EQSLRRLQTDHIDLFYCHRWDDTTPIEETLRGLDDLLRMGKVNYIGASLYASWQLAHA
                     NVLAELKGWTRFAVLQSEYNLFQRGVEREILPYCHAHKVGFVPYYPLAGGFLTGKYEM
                     GKPPPPGSRGESVKLVQNLMHETNYRLVAKLSDWAKDHGRGVNELAQAWLLAQPSVCS
                     VITGAKRVDHVTSNVKAAGWHLTGAELKEIDSILQAQQS"
     gene            complement(1548..1913)
                     /locus_tag="VMZ02_06390"
     CDS             complement(1548..1913)
                     /locus_tag="VMZ02_06390"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HUR71595.1"
                     /translation="MQRIHTSKDPLMISHLKNVLATFGITCVMKKMDLISAAGQLPPT
                     ECWPELWVIEDEKAVRAKAILKQTLAPLVSVKKAWVCRNCQEKIEGQFSECWNCGCNR
                     SGAQARGVVTMASRSRGRH"
     gene            complement(1930..2274)
                     /locus_tag="VMZ02_06395"
     CDS             complement(1930..2274)
                     /locus_tag="VMZ02_06395"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HUR71596.1"
                     /translation="MSERTIKGYHAHIYYDPARTRVAAEKVRAGLAQFNVQLGSWHDG
                     PVGPHLDAMYQALFSQDEFAKVVPWLMLNREGLSVLVHPSTGEAYGDHMERSLWLGER
                     LKLNEEVLRRAH"
     gene            complement(2433..2627)
                     /locus_tag="VMZ02_06400"
     CDS             complement(2433..2627)
                     /locus_tag="VMZ02_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71597.1"
                     /translation="MNPGDPLKTTKTIRSQKTGTILPRQGTFVRAVENMGRQLILVNF
                     GPAGEEYLFPDEIVPEAAKA"
     gene            complement(3264..4268)
                     /locus_tag="VMZ02_06405"
     CDS             complement(3264..4268)
                     /locus_tag="VMZ02_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71598.1"
                     /translation="MKQLITLLAVLMTVVSSSAAAYAQKLQQVRVALSTPTPHMAPLF
                     VAKDKRLYDKYGLDVQLILVNSGSLVAQMFQAGEIQITANAPASLVSLAATGEKLSFF
                     LGLSNTSPFSVISQPSMRRAEDLKGKKIGTARFGGSSHISALIALEHLGLDLKRDKIT
                     LIQTGVDPDRMVALENKAIDAGMLQRVATKVMTAKGFYNLLNMHAAKLQYQNTGLTVK
                     KDYASANPKVVDGFTRATIEAYAYIFKKENKQSVKEVVAKNLRLKNVEAAEDFYLEAQ
                     EELDRKPYPTLEGFKIVIKYVAEQNAKAAAIKAEEIVDSSWLKRLDDEKFFDRVYGGK
                     "
     gene            complement(4312..8676)
                     /locus_tag="VMZ02_06410"
     CDS             complement(4312..8676)
                     /locus_tag="VMZ02_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR71599.1"
                     /translation="MLWGLPAALFSLIGAIPLILFLHSLKPRGLKIATTTMFIWQRVL
                     RERPLGTRLGWLLRKNLLLILQLLAAVALIVALADPSLLHIASRSGDVIVVLDLSASM
                     KAKGKTASRFDGLRREFNSLIDGLSADQKMLVIGAGAEPRLLLPFSSDKRRLRDLARN
                     LAATDAPGKMKEAILFAHGFLKRGSTDHVTVISDGAFAGAEEFATAASQLQLVKVDGG
                     SENVGIVGFAVRRQPESPGAAEILVHVRNFTSKAVHLPLALTLDDKPILREEIDIGAN
                     DRRVLIYPLDGALAGRLVAQLEFTDDFSTDNRAYLALADTPLRVLYVGPGNPYLDKLF
                     RSFANLELTSLKVWDDHAAAQRSQFDVVICDRVAPPALVQGNLILIDTLPAQTALARQ
                     AKVEHPRLLLPLINHPLVAGLNLAEMQVRQATPLRDSGAGVALARIAQGPLIYALEQD
                     KLRLVYFGFDLLQSDLPLRVAFPVLFHNIFEWFQPQPLEFPSRSGQAGAPIKIGTAQI
                     NAEAAVTTPSGRTETFSSTSGSLVFADTFEAGFYRYSNGQRQGEFAVNLLNEDESQIV
                     SRLNANAPMQKTAAAVRSETGFSLWPMLLGAGLLLLGAEIFLAYRQGVSVYPLALRTG
                     ALALLAVALFNPRWFQPSAALDVIFAVDLSRSVGQEGREKAREVMEVAAAFKKTTTRT
                     GLMSFGSAPEWELPPRDEIAPVEFSARLDREQTDIQAALQGAAAQVGDGRQGRILLLS
                     DGNQNRGETARVMPLLRSQGVQVWTLPVGLSRGRNEIYLADLILPRQVDSAESFEIRV
                     VVESVREAPARLRLLRDGVLSAERELRLNAGSNDISFRDALNERGNHRYEVLIESVED
                     TLAENNLLQGVVDVKGPPRVLLLSAQKDSQRVLARVLQIQGYAVVEATPEAAGLSLAD
                     LAAFDLLVLDNVPAFQLTHAKMESIEKYVRDFGGGLLVIGGSQSYGAGGYYRTPLERV
                     LPVDMRPPAKLEMPHVALLFVIDKSGSMGSGAEGSTKLDLAKAAAIAAADIMNPTDQV
                     GILAFDANWDWALPFRQVGKGEWITEKLANLQSDGGTDLYKAMLEAYRGIAAKTASIK
                     HVIALSDGLTDKADFQSLVAKMAHDGITVSTVSIGNDADVKLMAEIAKDGKGRGYVAL
                     DPQTIPQIFTTETLLISRDLLIEKPVTASIVTATGALKGIAPSNLPTLRGYVLTYPKP
                     RAELLMRADKDPLLASWRYGLGRVMAFTSDLSGRWGREWVAWNVFPQWASQISRDTMR
                     KVLDGKMRVDFHAAGETVKVVTDLVSQEGKFLNHLKLKATIAVPNDTSLQRALQQSAP
                     GRYEGTFTPAQRGIHFVTLYAENSSGAAPLPVATVPYIAPYPKEYRELKPNLALLSRL
                     AEETGGEMLDPEKFHAGLKRLYTPVPGKGAKGQETWWPLASLGLLLFLADLVMRTWPW
                     RRAALDAPGHGLLPNVAG"
     gene            complement(8680..>9265)
                     /locus_tag="VMZ02_06415"
     CDS             complement(8680..>9265)
                     /locus_tag="VMZ02_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71600.1"
                     /translation="KIDYAKKVAAALGYIGLKNLDRVGGAAFSSVLHKPLTLGRGRKQ
                     ILSLFNFLAKLSCTAETNLRAAIHSFTTLFPHTGLVVIVSDFFDPSGWRAALEELSGK
                     NYQVLLVHVVDQAELKPHAWGDVALVDVEAGGERKFFVDTDLVRRFERELEIFFQEIE
                     SLCTSHKIDYLRTTTQIPFDEFVLQTLRRVSSVA"
BASE COUNT         2033 a         2665 c         2563 g         2004 t
ORIGIN      
        1 tggccggact tcgccgagtc cgtacgcgcc ctctgcatcg ctgccatcga caaacgccgt
       61 actcagcgcg agtacatcca gtggctggaa aaagaaatta gccgcttggc caaagcgcct
      121 aagcgcaagc aattgaccgg ctcagcacag ttgcttttgc cgttcgtgat ttgagcaacc
      181 gccaccgtca cagccggaaa tgtcacgaca ccgcgcggtg actggacttt ggcttattat
      241 tcacggaatg gcgttgcgtt cttcgcgtcg gggttaataa ggccgccgca aaacttcggg
      301 cctgatctct gcgccttaac tttgctgcgc ctgtaaaatg ctgtcgatct cttttaactc
      361 cgcgccggtc agatgccacc ccgctgcttt tacattgcta gtcacatgat cgacgcgctt
      421 ggcacccgtg atcaccgagc acacactcgg ctgtgccaac aaccaagcct gcgccaattc
      481 attgacgccg cggccatgat ccttcgccca atcgcttagt ttggcaacta agcggtaatt
      541 ggtttcgtgc atcagatttt gcaccaactt caccgattcg ccgcgcgagc ctggcggcgg
      601 cggtttgccc atctcgtact ttccggtgag aaagccgccg gcgagcggat agtacggcac
      661 gaagccgacc ttgtgagcat gacagtaagg cagaatttca cgctcgacgc cgcgttggaa
      721 caggttgtat tcggattgta atacggcaaa gcgcgtccag cctttgagtt ccgcaagcac
      781 attggcatga gccaattgcc atgaggcata cagcgacgcg ccgatataat tcactttgcc
      841 catgcgaagc agatcgtcca ggccgcgcaa cgtctcttcg atcggcgtgg tgtcgtccca
      901 ccgatggcag taaaacagat cgatatgatc ggtctgtaaa cgacgcaaac tttgctcgat
      961 ggactgcatc atttgatagc gcgaggcgcc ccagctgttg gcgctggtct tgcgcgggaa
     1021 attaaactta gtcgccatca cgacgttgtc catgcgtcct ttaagcgcca cacctaaagt
     1081 ttcctcggag cggccttcat tgtaaacgtt ggctgtgtcc aaaaaattca cgccggcatc
     1141 gagggccacg tcaattactt tattgacctc gctctgcggc atatccgcag aaccgaaacg
     1201 attggtcccc aagccgacga cggatacgcg cacaccggtg ttgcccatct gccgatattc
     1261 catcaaatct ccttgcacct gcttgatcgt taatattagt cagtgctggc aataagcctg
     1321 gcagaatcac ggcgatttct aacatccaag tattagtcgg tcaaactaac tggcgatgcg
     1381 actgcctcgc gctagtaatg agcttatgcg cgaacactct caggtgggtg cgctcggttt
     1441 gattcgactt ttgcgtgaat tgagaaaatt aatttgcagc aatcgacagc cgcgccgtga
     1501 tgacggaaac tatcttgctg tcgtggaaga ggtaagtgac agagaaacta gtgccggccg
     1561 cgcgaccgag aagccattgt gactacgccg cgggcctggg caccggaacg attgcagccg
     1621 cagttccagc actcggagaa ttgcccttcg atcttctcct ggcaattcct gcaaacccaa
     1681 gccttcttaa ccgatacaag cggcgccaaa gtttgcttga gaatcgcctt ggccctgaca
     1741 gctttttcat cctctataac ccacagttcg ggccagcact cggtgggggg cagttggcct
     1801 gcggcactga tcaggtccat ttttttcatc acacatgtta tgccgaacgt cgccaggaca
     1861 ttctttaagt ggctgatcat gagggggtct tttgacgtgt ggatgcgttg catcggatta
     1921 gccaaattct cagtgtgcgc ggcgcaagac ttcttcgttc agtttcaaac gctcaccgag
     1981 ccacagcgag cgctccatgt ggtcaccgta tgcctcaccg gtggacggat gtaccaatac
     2041 agacaaacct tcacggttga gcatcaacca agggactacc ttggcgaatt catcctgaga
     2101 gaacagcgct tgatacatgg cgtcgagatg tggtcccacc ggaccgtcat gccagctgcc
     2161 taactggacg ttaaactggg ccaagccggc acgcactttc tccgcggcga cacgagtccg
     2221 cgccggatcg taatagatgt gagcatgata gcccttgatc gttcgctcgc tcatcgccca
     2281 cctccacaaa atgtgtaaga gtttctatca gaaacgactg ttgatgccaa agcgaccgag
     2341 cgattgggtg agattccgat ggcaatagaa gagcgttggg ctcccgtctt aagggcgggg
     2401 agcaaaccgt tgcggaatgg aaacggcagg atctaagctt ttgcggcttc aggcacaatt
     2461 tcgtccggga acaagtactc ttcaccagcc ggaccgaaat tcaccagaat caactgccgt
     2521 cccatattct cgacagcccg gacaaaggtc ccttgccgag gcaagatcgt gccggttttc
     2581 tgcgagcgaa tggttttggt agttttgagc ggatctccag ggttcattaa ttctccctcc
     2641 cggtgtggat gagctcagtt tggttctttc ttgtttatcg tgtgcttaag cttttctctc
     2701 cttaacgcgc agtgacgttc actgtttaga cgcccgtgag agacgtttaa tgcaaggaaa
     2761 agaccagggt gaatcgactg gcgggatcgt cactagagcg gcggtcaaaa atatcacttg
     2821 aaatctatat aaccacaaaa ctcggtgaaa ttaaagaaaa tttttgtagt gtgagcgttt
     2881 gtcgtcgttc caattcgcgg tcgggggata tatttcaagg gtaagttgcc actcaagtag
     2941 gcacaagctg ctaaactatt cgtcccacgc cggcggaatt tgtcttattc actgtaagat
     3001 ttttagccaa ccgcgcacat tgcctcttaa ccacttataa cccaatcaaa atagcgacca
     3061 tcgttgggaa gcagccagcg gctagcaata gaattctatt ggcggagaat aaccaacctc
     3121 tggatgctgt caatttctta tcgcaaggcg tgccgtggcg acgatctaga attgttgatc
     3181 aagacgagca aagcgaacgt aataagacca gtcgctatca ctccgacacg atagccgctc
     3241 gccgaaaatc gatctctacc gaactacttc cccccataaa cccggtcaaa aaacttctcg
     3301 tcatcgagtc gcttcagcca gctgctatcg acgatctctt cggctttgat ggcggcagct
     3361 ttagcatttt gttcagcgac gtatttgatg acgatcttga agccctccag ggtcggatag
     3421 ggcttgcggt cgagttcttc ttgagcttcg aggtagaaat cctccgccgc ctcgacgttc
     3481 ttcaagcgaa ggtttttcgc gacaacttct tttaccgact gtttgttttc tttcttgaag
     3541 atataggcat acgcctcgat ggtcgcgcgg gtaaagccgt cgacgacctt aggattggcc
     3601 gaggcatagt cttttttcac cgtcaaaccg gtgttctggt attgcagctt cgccgcgtgc
     3661 atgttgagca gattgtaaaa acccttagca gtcatcactt tggtggcaac gcgttgcagc
     3721 atgccggcat cgatggcttt gttttccagc gccaccatgc ggtcgggatc gacgccggtt
     3781 tgaatcaagg taatcttgtc gcgcttgaga tcgaggccca agtgttcgag cgcgatcagc
     3841 gcggagatat gcgacgagcc gccgaagcgc gccgtgccga ttttcttacc cttgagatct
     3901 tcagcacggc gcatgcttgg ctggctgata accgagaacg gtgaagtgtt gctcagcccc
     3961 aaaaagaaac tcaacttctc tcccgtggcg gcgagactga ccagcgacgc aggcgcattg
     4021 gcggtgatct gaatttcgcc ggcttgaaac atttgcgcca ccagcgagcc gctattgacc
     4081 agaatcagtt gtacatcgag accatattta tcatagagtc tcttgtcctt ggcgacaaac
     4141 aacggtgcca tatgcggcgt cggcgtcgat aacgctacgc gcacctgctg gagcttctgc
     4201 gcgtaggctg ccgcactgct gctaaccacc gtcattagaa ctgcaagtaa cgtgatcaat
     4261 tgtttcaccg gaactcctcg cgttgaccta caactataat aacaaatgaa tctacccggc
     4321 gacgtttggg agcaatccgt ggccaggcgc gtcgagtgct gcgcgacgcc acggccaagt
     4381 acgcattacc aggtcggcga gaaacagcaa caggccaagg ctcgccaacg gccaccaggt
     4441 ctcttgcccc ttggcgcctt tgcctgggac cggcgtgtaa aggcgcttta aaccggcatg
     4501 aaatttctcc ggatcgagca tctcgccgcc ggtttcctcg gctagccggc tgagcagcgc
     4561 aaggttgggt ttcaattcgc ggtactcctt ggggtatggt gctatgtaag gcaccgtcgc
     4621 aactggcagg ggcgccgcgc cgctgctatt ttcagcgtaa agcgtgacga aatgaatgcc
     4681 gcgttgggct ggtgtgaacg tcccctcgta gcgcccgggg gcgctttgct ggagcgctcg
     4741 ctgcaggctg gtatcattgg gaaccgctat tgtcgccttc agtttgagat ggttcaaaaa
     4801 tttgccctct tgagaaacca agtcggtgac gacttttact gtctcaccgg cggcgtgaaa
     4861 atcaacgcgc attttgccgt cgagcacctt gcgcatggtg tcgcggctga tctgactcgc
     4921 ccactgggga aacacgttcc acgccaccca ctctctaccc cagcgcccgc tcaggtcgga
     4981 tgtgaaggcc attactcggc cgagaccgta gcgccaggac gccagcagcg gatccttatc
     5041 ggcgcgcatg agcaactcgg cgcgcggctt gggataggtc agcacataac cgcgcagtgt
     5101 cggcaaattg cttggcgcga tgcctttgag cgctccggta gcagtgacaa tgctcgccgt
     5161 gaccggcttc tcgattaata aatcgcgtga gatcaacaat gtctcggtgg taaagatctg
     5221 cggaatcgtt tgcggatcga gcgcgacata gccgcgtcct ttaccgtctt tagcgatttc
     5281 cgccatcagt ttgacgtcgg catcgttgcc gatcgagacc gtcgatacag tgatgccgtc
     5341 gtgcgccatc ttggctacca gggattgaaa gtcggccttg tcagtaagac cgtcggagag
     5401 cgcgatcacg tgtttgatag aagccgtttt agcggcgata ccgcgatagg cttcgagcat
     5461 tgctttgtac aagtcggtgc cgccgtcgga ctgcaaattg gccagctttt cggtgatcca
     5521 ctcgcctttg ccgacctggc gaaacggcaa cgcccaatcc caattcgcgt cgaaagcgag
     5581 gatgccgacc tgatccgtcg gattcatgat gtcggcggca gcgatggcag ccgccttggc
     5641 gagatcgagc ttggtgctgc cttcggcgcc ggagcccatg ctgcccgact tgtcgattac
     5701 gaagagcagc gccacatggg gcatttccaa tttggccggg ggacgcatgt cgacaggcaa
     5761 gacacgctcc agcggcgtac ggtagtagcc gccggctcca tagctctgcg agccgccgat
     5821 cacgagtaac ccgccgccaa aatcgcgcac gtacttttcg atgctctcca tcttggcgtg
     5881 cgtcagttga aacgccggca cgttgtcgag caccaacaga tcgaaggcgg cgaggtcagc
     5941 gagagacaag ccagctgcct ccggcgtcgc ttcgacgact gcgtagcctt gaatctgcag
     6001 cacacgtgca agaactcgct gactgtcttt ctgcgccgac agcaatagca cccgcggtgg
     6061 tcctttaacg tcgacaacac cttggagcag gttattttcc gccaaggtat cttccacgga
     6121 ctcgatcaag acttcgtagc ggtgattgcc ccgctcgttg agcgcatcgc ggaacgagat
     6181 atcgttgcta ccggcgttca aacgcagctc gcgctcggcg ctgagcacgc cgtcgcgcag
     6241 caaacgcagc cgcgccggcg cctcgcgcac gctttcgacg acaacacgaa tctcgaagct
     6301 ctcggcgcta tcgacttggc gcggcagaat taaatccgcc aaatagattt cgttgcgacc
     6361 gcgcgacaag ccaacgggca gcgtccatac ctgcacgcct tgactgcgca ggagcggcat
     6421 cacccgcgcg gtctcgccac ggttttgatt gccgtcggat aaaagcaaaa tgcgcccctg
     6481 gcgcccatca cctacctgcg cggctgcgcc ttgcagggcg gcttgaatat cggtctgctc
     6541 acgatcgagc cgcgcggaaa actccacagg cgcaatctcg tcgcgcggcg gcaattccca
     6601 ttcgggagcg ctgccaaagc tcatcagccc agtgcgcgtc gtcgtttttt tgaaggcggc
     6661 ggcaacttcc atgacctcgc gcgctttttc ccttccctcc tggccgacac tgcgcgacag
     6721 atcgacggca aagatcacat cgagcgccgc gcttggctga aaccaacgcg gattgaacag
     6781 cgccacagcg agcagcgcca gtgccccggt gcgcaaggcc agcgggtaca cggacacacc
     6841 ttggcgatag gccaaaaaaa tctccgcgcc gagcaacaac agacctgcgc cgagcaacat
     6901 cggccataaa gaaaagccag tctcggaacg caccgccgca gcggtctttt gcatcggagc
     6961 gttggcgttc aagcgcgaga cgatctgcga ctcgtcttca ttgagcagat taacggcgaa
     7021 ttcgccttgc cgctggccgt tgctataacg ataaaacccc gcctcgaaag tgtcggcaaa
     7081 cacgagcgac ccgctggtgc tggaaaaggt ttctgtcctg ccgctcggcg tggtgacggc
     7141 cgcctctgca ttgatttgcg ccgtgccgat cttaatcggc gcgccggctt gcccgctccg
     7201 gctggggaat tcgagcggct gcggctggaa ccattcaaaa atattatgaa acaaaaccgg
     7261 aaaggcgacg cgcaacggca gatctgactg cagcaaatca aagccaaaat agaccagccg
     7321 cagtttgtct tgttcgagag catagatcag tggtccctgc gctatgcgcg cgagcgcgac
     7381 gccagcgccg gaatcgcgca ggggcgtggc ttggcgcact tgcatttccg ccaagttgag
     7441 tccggcaacc agcgggtgat ttattaacgg caaaagcagc cgcgggtgtt cgactttggc
     7501 ctgacgcgcg agcgccgttt gcgccggcag cgtatcgatt aatatgagat tgccctgcac
     7561 caatgccggg ggcgcgacgc gatcgcagat tacgacatca aattggctcc gttgtgcagc
     7621 ggcgtggtca tcccaaacct tcagcgaggt cagctcgaga ttagcgaagg aacgaaatag
     7681 cttatccaaa tagggattgc cggggccgac gtagagcacg cgtagcggcg tgtcggccaa
     7741 agcaagatag gcgcgattgt cggtgctgaa atcgtcggta aactctagtt gcgccaccag
     7801 cctccccgcc agcgcaccgt ccaagggata gatgagcacg cgccggtcgt tggcgccgat
     7861 gtcgatttct tcgcgcagaa ttggtttatc gtcgagcgtt agcgccagcg gcaggtgcac
     7921 cgccttcgaa gtaaagttac gcacatgcac caggatctcc gccgctcccg gggattccgg
     7981 ctgacggcgc acggcaaaac cgacaatgcc aacattctcg ctgccgccgt cgactttgac
     8041 caactgcaac tgcgacgcgg ctgtagcgaa ttcctcagcg ccggcgaaag ctccgtcgct
     8101 gattacagtt acatggtcgg tactgccacg cttgagaaac ccatgggcga agagtatcgc
     8161 ttctttcatt ttccccggag cgtcggtggc cgccaagttg cgcgccagat cgcgcagccg
     8221 ccgtttgtcg gagctaaacg gcagcaagag ccgtggttca gcaccggcgc cgataaccag
     8281 cattttctga tcggccgaaa ggccatcgat cagtgaatta aactcacggc gcagtccgtc
     8341 gaaacggctg gcggttttgc ccttggcctt catgctcgcg ctcaagtcga gcacgacgat
     8401 cacgtccccc gaacgggaag cgatgtggag cagcgacggg tcggcgagag cgacaatcag
     8461 cgcgaccgcg gcgagcaatt gcagaatcag caataggttc ttgcggagga gccaacccaa
     8521 gcgcgtgccc aacggccgct cgcgcaacac ccgctgccaa atgaacatcg tggtagtcgc
     8581 gatctttaga ccgcgcggct tgaggctatg aagaaatagg ataagcggga tcgccccgat
     8641 gagtgaaaac agtgcggctg gcaatcccca gagcatcgtt caggccacgc tgctcacccg
     8701 gcgcagcgtt tgtaacacga actcgtcgaa gggtatttga gtcgtcgtgc ggagatagtc
     8761 tattttgtga ctggtacaaa gggattcaat ctcctggaaa aagatttcca gttcccgctc
     8821 gaaacggcgc accagatcgg tgtcgacaaa gaacttgcgt tcgccccccg cctcgacgtc
     8881 caccagcgcg acgtcgcccc aggcgtgagg ttttagctcc gcctggtcga ccacgtggac
     8941 caacaaaact tgatagtttt taccagagag ctcttccagc gcggcgcgcc agccgctggg
     9001 gtcgaaaaaa tcgctgacga tgacgaccaa ccccgtatgc gggaaaagag tcgtgaagga
     9061 atgaatcgcg gcgcgcaagt tggtctccgc cgtgcaggat agtttggcga gaaagttaaa
     9121 aaggctgagg atctgcttac ggccgcgccc caaagtcagc ggcttgtgca acactgaaga
     9181 gaatgccgcg ccaccgaccc ggtcaagatt tttgagcccg atgtagccca acgccgcggc
     9241 gactttcttg gcataatcga tcttg
//
LOCUS       DATLCH010000217         4599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4291061, whole genome shotgun sequence.
ACCESSION   DATLCH010000217 DATLCH010000000
VERSION     DATLCH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4291061"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..783
                     /locus_tag="VMZ02_06420"
     CDS             232..783
                     /locus_tag="VMZ02_06420"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HUR71601.1"
                     /translation="MIPKKCFFTKGVGVHKEKLASFELALRQAGLAYCNLVLVSSIFP
                     PYCKRLSKEEGAKLLRPGEVVFSVYDRESTNEPNRLVAASVGVAIPSDESQYGYLSEH
                     HSFGETDEKAGDYAEDLAASMLATTLGIEFNPDTAWDEREQLFKMSEKIVRTSNITQS
                     AIGNKDGLWTTVFAACAFINDDN"
     gene            complement(947..1714)
                     /gene="tatC"
                     /locus_tag="VMZ02_06425"
     CDS             complement(947..1714)
                     /gene="tatC"
                     /locus_tag="VMZ02_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HUR71602.1"
                     /translation="MPDGKGGPADAQMPFTSHLGELRSRLIKSVLAVGVAFLGCFYFV
                     DAIFAVLAAPLRRLKIDGLILIGTAVTEAFFTKMKIGLFAALGVALPVLLWQAWQFVA
                     PGLYEHEKRYTRSFVFAGSLFFCAGAAFCYVILIQQGLSFLLHRYEVIGVKPMLQVGD
                     YLSTVSRMVLLSGAMFELPVLAFFTARVGLIDHRFLIRHSRYALIAIALVAAILTPPD
                     LLSQILLMVPLCGLYLVSIAVAYLARFRMNRLDRAAD"
     gene            complement(1834..1995)
                     /gene="tatA"
                     /locus_tag="VMZ02_06430"
                     /pseudo
     CDS             complement(1834..1995)
                     /gene="tatA"
                     /locus_tag="VMZ02_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            2281..3309
                     /locus_tag="VMZ02_06435"
     CDS             2281..3309
                     /locus_tag="VMZ02_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HUR71603.1"
                     /translation="MAQTAVVIDKEYLKHQPGDGHPERPERVKVLLDLAESLDPAKFA
                     LLPPLPAQRADIEATHGTDHVKLIESTARHNRYALDGDTITCRDSFAVSLLAVGGFIR
                     LLDAIAANESRNGFALVRPPGHHALRDRAMGFCLFNTMVIGAEYLKRVHNVRKILIMD
                     WDVHHGNGTQDAFYADPSVMFISTHQYPYYPGSGAANETGIAAGEGFTINIPLPAGCA
                     DAEYAQVFRDIIEPAAEKFVPDWILVSAGFDPHRRDPLGGMGVTEQGFALMSRRLLAL
                     AEKFTDAKIAFLLEGGYDLAGLRGSVAAVLETMQVGTAPAPDALDPNASRIAPLVRHI
                     QQLHERYQ"
     gene            3393..>4599
                     /gene="fabF"
                     /locus_tag="VMZ02_06440"
     CDS             3393..>4599
                     /gene="fabF"
                     /locus_tag="VMZ02_06440"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HUR71604.1"
                     /translation="MNTTEARRVVVTGMGLVTPLGTGVDKNWDALMAGRSGIRKISRF
                     NAEPFASRIAGEVPDFRAEDYIEPKEIKKMDLFIQYAMGATAQAMADSGLKIDGEFAE
                     NVGVIIGVGMNGLDTLEVTKEALMAGGPRKISPFFIPKVISNLAPGHVAIRYGAKGIN
                     FTPTSACASGTHAIGEAYHMIRRGLQDAVIAGGAEAAITPLGVGGFSAMKALSTRNDE
                     PERASRPFDKERDGFIIGEGSGILILEEREHALQRGATIYAEVIGYAANGDAHHMTAP
                     APNGEGAARCMRLALKDAGIAPSDVSYINAHGTSTEYNDANETTAIKTVFGEAVAKLA
                     VSSTKSMTGHLLGAAGAIEAVYSALALQHGMLPPTINYENPDPACDLDYVPNQARKAD
                     VKVVLSNSFG"
BASE COUNT         1046 a         1260 c         1306 g          987 t
ORIGIN      
        1 tccgaaggcg atcaagtcga aaaaggccag ggactggtaa tcgtcgaggc gatgaagatg
       61 gaaaacgaag tgcgtagtcc catcgccggt gaaatcaaag agatcaaagt caaaccgggc
      121 gacaccgtcg aaggcggcgc gctgctagtg gtagtcgagt agaactattt cacgagatta
      181 tcgcggagcg attcttgtca ggttaaatct ttcgtgttat agcagcgcac catgatcccg
      241 aagaaatgtt tttttaccaa aggtgtcggc gtccataaag aaaagctggc gtcgttcgag
      301 ttggccttgc gtcaggccgg actggcttac tgtaatctcg tgttggtatc gagtatcttt
      361 ccgccttact gcaaacggct ttccaaggaa gaaggggcga agctccttcg acccggtgag
      421 gtggtgttca gcgtctacga tcgggagagc accaacgagc ccaaccgcct agtggcagcg
      481 tccgtcggtg tggcgattcc gtcggacgaa agccaatacg gttatctgtc ggagcaccat
      541 tcatttgggg aaaccgatga gaaggcgggc gactatgccg aggatcttgc cgccagcatg
      601 ttggcgacga ctctagggat cgagttcaat cccgacacgg cttgggacga acgcgaacag
      661 ctcttcaaaa tgtccgaaaa aatcgttcgt acttccaaca tcacacagtc cgccatcggc
      721 aacaaagacg gcttatggac aacggtgttc gccgcctgtg cgtttatcaa cgacgataat
      781 tgaaagttta ggcgtcatcg ggtaatcagg ctgggcgcta ccttcgggtg cgcccttttt
      841 gtttatgccg tactttgcac ttcgcgaagt tgctatttat ttgcgttgca gagcacgtgc
      901 gaccaggcgg cgagcggatt tgcagccatg gttgcctaat ccgcggttag tccgccgccc
      961 ggtccaaccg gttcattcgg aagcgcgcta agtaagcgac tgcgatactc acgagataaa
     1021 gaccacaaag cggaaccatc aacaaaatct gcgagagcaa gtcggggggt gtcagaatcg
     1081 ccgcgacgag cgcgatggca atcagcgcgt agcggctgtg gcggatcagg aaacgatgat
     1141 cgatcaggcc aacccgcgcg gtgaaaaacg ctaggaccgg cagttcgaac atggcaccgc
     1201 ttagcagaac catccgtgat accgtcgata agtagtcgcc tacttgcaac atcggcttga
     1261 cgccgatgac ctcgtaacgg tgtaacagga agctcaatcc ttgttgaatc agtatgacgt
     1321 agcaaaacgc tgcgccggcg cagaagaaca gcgagccggc aaagacgaag ctgcgtgtgt
     1381 agcgtttttc atgttcataa aggccgggag caacgaactg ccatgcctgc cacagtagaa
     1441 ccggcagagc gacacccagc gcggcgaata agccgatttt catcttggtg aagaatgctt
     1501 ctgttaccgc ggtgccgatc aaaatcagtc cgtcgatttt gaggcggcgc agtggtgcgg
     1561 cgagaactgc aaagattgca tcgacaaaat aaaaacagcc gaggaatgcg acgccaacgg
     1621 cgagaacgga tttgattagg cgtgagcgca actctcccag atgggaggtg aagggcattt
     1681 gagcatcggc gggccccccc ttgccatccg gcatcgtcac tttttctcgg acgtatcggg
     1741 attgatttcg gctggccctg tgcccgaggc ttgagcaact cgggactcct ctggtgttgg
     1801 aatcggcgtg ggttcagggt tgtctatatc cacttcgact ttgcgaaatt catctttgag
     1861 ctcgtcggtg gcccggcgaa actcggccaa ccccttaccc aaagttttcg ctagttcagg
     1921 tagttttttt gggccgagga cgatcaacgc cagtcccaaa atcaacaaca attccggcat
     1981 gccgatccca aacatgtagc taaactaagg gatttcactc ggctccgcaa gattgcgccc
     2041 gttgtcctgg ttattttgag atcgacgcct tcggagtacg gcgatatcac ccgccttgcc
     2101 tccgagggat gccaagcttt agcgcttcaa cgaaaagatc gccatcgatc ctttgacctc
     2161 gtcgaacaaa tcgaatcggc gatgaagacg gcagttttga cgccgagcgc tcgatgtcca
     2221 ttgaaaacta atagatcagt ccgctttgca gcgtctcggc gtcggtgtta ggttccgctt
     2281 atggctcaaa cagccgtcgt catcgataag gagtacttga aacaccagcc gggtgatggt
     2341 catccggagc ggcccgagcg ggttaaagta ctgctggatt tagccgagag tctcgatccg
     2401 gccaagttcg cattgttgcc gccactgccg gctcagcgcg ccgatatcga agcgacgcat
     2461 ggcacagatc atgtgaagtt gatcgaatcg actgcacgac acaatcgcta cgcgttggac
     2521 ggcgacacga ttacttgccg cgattccttc gcggtcagtt tgctggcggt gggaggattc
     2581 attcgtttgc tcgacgccat cgccgccaat gagagtcgca acggattcgc gctagtgcgg
     2641 cctccaggcc accacgctct gcgcgaccgc gccatgggct tttgtctttt caacacgatg
     2701 gtgatcggcg cggaatattt gaagcgtgtc cataacgtca ggaagattct catcatggat
     2761 tgggacgtgc accacggtaa tggcacacag gatgcctttt acgccgatcc ctcagtcatg
     2821 tttatctcaa cccatcagta tccgtactat cccggcagcg gtgctgccaa cgaaaccggt
     2881 atcgctgccg gcgagggttt taccatcaat ataccgctcc ctgcgggctg cgccgacgcc
     2941 gaatatgcgc aggtgtttcg agatatcatc gagccggccg cggaaaagtt tgtccccgat
     3001 tggattctgg tctccgcggg cttcgatccg caccggcgtg atccactggg cggcatgggc
     3061 gtcaccgaac aaggcttcgc actgatgtcg cgccgtttgc tcgcactcgc cgaaaaattc
     3121 accgatgcca aaatcgcttt tctgctcgag ggtggttatg atctcgccgg cttgcgcgga
     3181 tcggtggcgg cggtgctcga gaccatgcaa gtaggcaccg cccccgcgcc cgacgcactc
     3241 gatccgaacg ccagtcgaat cgccccgctc gttcgtcaca ttcaacagct tcacgaacga
     3301 taccaataac ctaaattcct attgattcac cgggaactct cctttatctt gaagtttaag
     3361 gttcaaattc atcaaaagat tggttaggca tcgtgaatac aaccgaagca cgcagagttg
     3421 ttgtgaccgg aatgggtctg gtcacgccgt tgggaaccgg cgtcgacaag aactgggacg
     3481 ctttaatggc cggacgctcc ggcattcgta agatcagccg ctttaatgcc gagccatttg
     3541 cttcgcgcat cgccggcgaa gtgcccgact tcagggccga ggactatatc gagccgaaag
     3601 agatcaagaa aatggatctc ttcattcaat acgctatggg tgccacggca caagccatgg
     3661 cggacagtgg tcttaaaatc gacggcgaat ttgccgagaa cgtcggtgtc attatcggcg
     3721 tcggcatgaa tggtctcgat acgctcgaag tcaccaaaga ggcgttgatg gctggcggac
     3781 cacgtaagat ctcgccgttt ttcattccca aagtgatttc caatttggcc ccgggtcatg
     3841 tggcgatccg ttacggcgcc aagggcatca actttacgcc gacgtcggct tgcgcttcag
     3901 gcacacatgc gatcggcgaa gcttatcata tgattcgccg cggtttgcag gatgcagtga
     3961 tcgccggcgg cgccgaagca gcgatcaccc cgttgggcgt gggcggtttc tccgccatga
     4021 aagccttgtc gacgcgcaac gacgagcccg agcgcgccag ccggccgttc gataaagagc
     4081 gcgatggttt catcatcggc gaaggctccg gtattttaat tctcgaagaa cgagaacatg
     4141 cgctccaacg cggcgcaaca atttacgccg aagtcatcgg ttacgccgcc aacggcgatg
     4201 ctcatcacat gaccgcgccg gcgcccaatg gcgaaggcgc ggcgcgctgc atgcgcctgg
     4261 cgttgaaaga cgccggcatc gcgccgagcg atgtcagtta tatcaacgct catggcacct
     4321 cgacggaata caacgatgcc aacgagacca cggcgatcaa gacggtgttc ggcgaagcgg
     4381 tggcgaagct ggcggtcagc tcgactaagt cgatgaccgg tcatttgctc ggcgccgcgg
     4441 gagcgatcga agccgtttac tcggcgctgg cgctgcagca tggcatgctg ccgccgacca
     4501 tcaattatga aaatcccgat ccagcctgcg atctggatta tgttcccaat caagcccgca
     4561 aggctgacgt gaaagtggtc ttgtccaact ccttcggtt
//
LOCUS       DATLCH010000218         5418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5801199, whole genome shotgun sequence.
ACCESSION   DATLCH010000218 DATLCH010000000
VERSION     DATLCH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5801199"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VMZ02_06445"
     CDS             <1..970
                     /locus_tag="VMZ02_06445"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR71605.1"
                     /translation="YNCSYHRMEVKDRNHTGFLMTLQHMWRIFRGYEEAGEECPVATV
                     IGHHPAYQMGACYGGRFEISEYDIIGGYMQEPLRLTPSETWGERFLVPADAEIVIEGA
                     LLPGKRMVEGPFGEVNGYMGVQGFQQSAHYEVRAITRRKGAMHTSILTPEGDKPWMDL
                     AREGAYLRRAREAVPNVVAVCTSGRHALLNVFIAMKKLSEGDPGRAAAAVLTWDWCKN
                     VFVFDEDINVYDPTEILWAIATRVQPHLQVSIIKEMMRGSLIDPSMVSPRKTSAMIVD
                     ATKPLDRPFSPVSKCPDDAMARVKLEDFVPGEVLQHIPIDRTTYWA"
     gene            1236..2222
                     /locus_tag="VMZ02_06450"
     CDS             1236..2222
                     /locus_tag="VMZ02_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HUR71606.1"
                     /translation="MSGIFISYRRDDSAPYAGRLCDRLGAVFGAEQVFMDVDDIPPGA
                     DFSAHIGAKIGGCDALLAVIGKQWLTARNAEDQLRLSDPDDLVSREIALALQRGVAVI
                     PVLVEGAGMPKAAELRGDLKPLAQRNALTLSDQNFHGDVAKLIKALELLPGLRKQVSR
                     TKDDWRSDMRQRLRRRLVWKIPLIIVLVGFAVWWQWRQQVTNGPAPNVTMGNSSIAAK
                     LTGRWRGTVTYPWGAKYQEEFFFTPEGNTLFGTASFIGIKRGIEEGRIAGDMITFKVR
                     FEETSSASTRIGTNRYEGQLEGDMLVLKFFDEAGSPPVEIKLTRVSDAANHP"
     gene            2292..3404
                     /locus_tag="VMZ02_06455"
     CDS             2292..3404
                     /locus_tag="VMZ02_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HUR71607.1"
                     /translation="MDQEIIARLRKRISAAGLDALIAISPENVTYVSGFVVPSQSLMR
                     WRHAACVVTADGKISMVAIDMEATTVKAHAGIDDLCIYREFSDDPMVKLAETLKAAKL
                     DRAKVGIEMEFLPAKDFATLQKNLSDVNWVAADAIFNKARQIKTPGELALLRSLSKLT
                     DNAIGTALRSAKIGMSEMELAGTLLTTLFAGGAESYKLMIIASGERSEFPNVGPSERK
                     LKHGDIIRMEIFGQKRGYLTGVCRTAVVGEATTEQYNIWSNLIECKYLVMDLIKPGAS
                     CPEIYRRFLEKFGQLGFDPISFVAHGIGLHLHEEPYMGRYGDEIVEAGMVGAFEPLVY
                     IPGRFGMQNKDMFCVTDRGCELLSDVTPTDTLLRVG"
     gene            3470..4624
                     /locus_tag="VMZ02_06460"
     CDS             3470..4624
                     /locus_tag="VMZ02_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HUR71608.1"
                     /translation="MKITNIETIPIRLPTRRVHQWASLTTPIGVYVIVKLYTDDGLIG
                     LGEAPVLKDWGGDHGKYFGETPQTTAHILGDILAPALAGQDPGRFETIHALMDKAVKG
                     YPYCKAAIDAALYDVVGKALNVPAYQLLGGLFRERIPIAHSLGLMEIEKAVNEALQAK
                     SEGVKTIKLKGGVEQKRDVELVRQIRQAIGSELNICVDANQGYPTAKAAVKIIKAMEE
                     FNLLYMEQPVEGIDQMAEVARRVDTPIMADESAWTAQDVLEIIHKKAADVISIYTTKP
                     GGMFKAKKVAALAEAAGMKCNVNGSVETGVGNAANIHLAASTGVVTYGCVVPVSTPKG
                     KGTGGIAGIYYHDDVIREAFNYADGDVIVSSKPGLGVELDEDKVRHYRID"
     gene            4799..>5418
                     /locus_tag="VMZ02_06465"
     CDS             4799..>5418
                     /locus_tag="VMZ02_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HUR71609.1"
                     /translation="MTIEKIAILGAGNGGCAAAADLTLRGFQIRLFSRSESTTVKLSK
                     VGEIELIEDGVGKKAAPFFISPHLPPVVQGVDLIVVTTPAVGHEYLAKGIAEYLSDGQ
                     KILLNPGHTGGALHFAHALRQAGCRADIQLCETVTLTYICRMPQFGRVEVYRRTTNLR
                     CAAFPGKYTATLIPEIQRVFPNVVPAANVMETGFANINAIMHPAGML"
BASE COUNT         1246 a         1430 c         1591 g         1151 t
ORIGIN      
        1 ttacaactgt tcttatcatc gcatggaagt taaggatcgc aaccacactg gtttcttgat
       61 gacgctgcaa catatgtggc gaatttttcg cggctacgaa gaggccggtg aagaatgtcc
      121 ggtggccacc gtgatcggcc accatccggc gtatcagatg ggcgcctgct acggcggccg
      181 gttcgagatc agcgaatacg atattatcgg cggctacatg caagagccgc tgcgtctcac
      241 gccttcggaa acttggggcg aacgctttct cgtgccggcg gacgcggaaa tcgtcatcga
      301 aggcgcattg cttcccggca agcgcatggt cgaaggcccg ttcggcgaag tgaacggtta
      361 catgggcgtg caaggttttc agcagtcggc ccactatgaa gtccgcgcga tcactcggcg
      421 caagggagcc atgcacactt ccattctcac gccggagggg gacaagccat ggatggactt
      481 ggcgcgcgaa ggcgcatatt tgcgccgggc gcgcgaggcc gttcccaatg tcgtcgcggt
      541 ctgcaccagc ggacggcatg ctctgctcaa tgtgtttatc gcgatgaaga aactctctga
      601 gggcgaccca ggccgcgccg cggcagcggt attgacctgg gactggtgca aaaacgtttt
      661 tgtttttgac gaagatataa acgtttacga tccaacggaa atcctctggg ccatagccac
      721 tcgcgtccag ccgcatctgc aagtttcgat catcaaggaa atgatgcgcg gcagcttgat
      781 cgatccttcc atggtgagtc cgcgcaagac ttcggcgatg atcgtcgatg ccacgaaacc
      841 cttggaccgc ccgttctcac cggtttcaaa atgtcccgac gacgccatgg cgcgagttaa
      901 attagaagat tttgtgccgg gtgaagtctt gcaacatata cctatcgaca gaactactta
      961 ttgggcatag cttaatcgtt caaggttcaa gggttcgaaa gttcagggtc ggatcgaccc
     1021 catgcgcgcg atccgctaac cttgaaccgc tccttctaac taaacccatt cggcgaacac
     1081 ggaagagtaa aaacgaaaaa ggctatgcta ttaacctgct agaggtcttt aagaaacagt
     1141 agcggctcgg aacgtccacg gaatatagcg tgctcgatgt tttcccaacg cgccagcggc
     1201 gcgctcgcta aagcgaacct aaaaccaaaa accacatgtc gggcatcttt atcagttatc
     1261 ggcgcgacga tagcgcgcca tatgcgggcc ggttgtgcga ccggttgggc gcggtatttg
     1321 gcgccgagca agtgttcatg gatgtcgacg acattccgcc tggagccgac ttcagcgctc
     1381 acatcggcgc taagattggc ggctgcgatg cattgctagc ggtgatcggt aagcagtggc
     1441 tcacggcgcg taatgccgag gaccaattgc gcttaagcga tcccgacgat ctggtcagcc
     1501 gcgaaatagc tctggcctta cagcgcggag tcgccgttat tcctgtgttg gtcgaaggtg
     1561 caggcatgcc caaagccgcc gagttgcgcg gcgatctaaa accgttggcg cagcgcaacg
     1621 cgctgacgct cagtgatcaa aattttcatg gcgatgttgc gaagttgatc aaagcgctcg
     1681 aattgctgcc gggcttacgc aagcaggttt cccggacgaa ggatgattgg agatccgaca
     1741 tgcgccagcg cttgcgccgg cggctggtgt ggaagattcc attaatcatc gtcctggtcg
     1801 gctttgccgt ctggtggcaa tggcgtcagc aagtgacaaa cggtcccgcg ccgaatgtga
     1861 cgatgggcaa cagttcaatc gctgcaaagc tgaccggacg atggcgcggc acagtaacct
     1921 atccatgggg tgccaagtat caagaggagt ttttctttac tccggagggc aacacgctct
     1981 tcggtaccgc gtctttcatc ggtatcaagc gcggtatcga agaaggccgt attgccggtg
     2041 acatgattac ctttaaggtg agattcgagg aaacgtccag cgctagcacg agaatcggaa
     2101 cgaatcgcta cgaaggccaa cttgagggcg acatgctagt gctaaagttc ttcgatgagg
     2161 cgggcagtcc gcctgtagag atcaaattga ctcgggtaag cgatgctgcg aaccatccat
     2221 gagtccgcgg tctccctgac gtattccctt gaccgtctcc gaaccagccg atattctccg
     2281 aaacaatttc catggaccaa gaaatcattg caagactgag aaagcgcatc agcgccgcag
     2341 gtctggacgc cctcatcgcc atctcgccgg aaaatgttac ctacgtgtct ggtttcgtag
     2401 tgccgtcgca gtcgttgatg aggtggcgtc atgcggcttg cgtcgttacc gcggacggga
     2461 agatctccat ggtcgcgatc gacatggagg cgacgacggt gaaggcccat gccggcatcg
     2521 acgatctgtg catctatcgc gaatttagcg atgatccgat ggttaaactg gcggaaactt
     2581 taaaagctgc aaaacttgat cgcgctaaag tcggcatcga gatggagttc ttgccggcta
     2641 aggacttcgc gacgcttcag aaaaatctgt cggatgtgaa ctgggttgct gccgatgcga
     2701 tcttcaacaa ggcccgccag attaaaacgc ccggcgaact cgcgttgctg cgctcgctca
     2761 gcaagctcac cgacaatgcc atcggcacgg cgctgcgctc ggcgaaaatc ggtatgagtg
     2821 aaatggagtt ggcagggaca ttgctgacga cactatttgc cggcggcgcc gagagctaca
     2881 agttgatgat tatcgcctcg ggcgaacgga gcgagtttcc caacgtcggc ccgagcgaac
     2941 gcaagctcaa acacggcgat atcattcgga tggaaatatt cggccagaag cgcggctacc
     3001 tcaccggcgt ttgccgcaca gcggtggtcg gtgaagcgac caccgagcag tacaacattt
     3061 ggtcaaattt gatcgaatgt aaatatttgg tcatggactt gatcaaacca ggtgcgagct
     3121 gtccggagat ctatcgaagg tttcttgaaa aattcggcca gttgggtttc gacccgatca
     3181 gctttgttgc ccatggcatc gggcttcatc ttcatgaaga gccgtacatg gggcgttatg
     3241 gcgatgagat cgttgaagct gggatggtcg gcgcgttcga gccgttggtt tacatccccg
     3301 gtcgcttcgg tatgcagaac aaagatatgt tttgcgtgac tgaccgaggc tgcgaactgc
     3361 tttccgacgt gacgccgacg gacacgctgt tgagagtcgg ttaataaaaa ccctcacgct
     3421 cgccctctcc catcggatgg gcgagggaat gtggaattgt tgaaattaga tgaagattac
     3481 aaacatcgaa accattccta ttcgcttacc gacacgccgg gtccaccagt gggcgagcct
     3541 gacaacgccg attggcgtct acgtgatcgt taaactttac acggatgacg ggttgattgg
     3601 tctcggtgaa gctccggtgc tcaaagattg gggcggtgat catggcaagt atttcggcga
     3661 gacgccgcag accacggccc atatcctagg cgacattctc gcgccggcgc tagcaggcca
     3721 agaccccggc cgttttgaaa cgatccacgc gctgatggac aaggcagtga agggttatcc
     3781 gtactgtaaa gcagccatcg atgcggcgct ctacgatgta gttggcaaag ctttgaacgt
     3841 gccggcttat caattgctcg gcggcttgtt tcgcgagcgg attccgatcg cccatagttt
     3901 ggggttgatg gaaatcgaaa aagccgtcaa cgaagcgctc caagcaaaat cggaaggcgt
     3961 gaaaaccatc aagctcaaag gcggcgttga gcagaagcgc gatgtcgaac tggtcagaca
     4021 gattcgtcaa gccatcgggt cggaattgaa catctgcgtc gatgccaatc agggttatcc
     4081 gaccgcaaag gcggcggtga aaatcattaa agcaatggaa gaatttaatc tgctctacat
     4141 ggaacagccg gtggaaggga tcgatcagat ggcggaggta gcgcgccgcg tcgatacgcc
     4201 gatcatggcc gacgaaagcg catggaccgc gcaggatgtc ctcgagatca ttcacaagaa
     4261 agcggcggac gtcatttcga tctataccac taaacccggc ggcatgttca aagccaagaa
     4321 agttgcggcg ctggccgagg cggcaggcat gaagtgcaac gtcaatggtt cggtggaaac
     4381 cggggtcggc aacgcggcca atattcatct cgctgcttca accggcgtgg tgacctacgg
     4441 ctgtgttgtg ccggtgtcga cgcccaaagg caaaggcaca ggcggcatag ccggcattta
     4501 ctatcacgac gatgttatca gggaagcttt caactatgcc gacggcgacg tgatcgtgtc
     4561 gtcgaaaccc ggactgggtg tcgaactcga cgaggataaa gtcaggcatt atcggatcga
     4621 ttagattgat ggattctgtc caggcatgag cagcgccatt catcgcaatg cgaaaagggt
     4681 catccgtcat accggcggaa gccggtatcc attcttgttg ccaacgcccc gccctatctg
     4741 gataccggcc ttcgccggta tgacaaaacc gcgcgttggc tcgtgaaccg cagcgaatat
     4801 gaccattgaa aaaattgcga tcctcggcgc cggcaatggc ggttgcgcgg cggcggcgga
     4861 tctcacgtta cgcggttttc aaatccggct gttctcgcgc tcggaaagca ccaccgtcaa
     4921 actaagcaaa gtcggcgaaa tcgagttgat cgaggatgga gtcggcaaga aggcggcgcc
     4981 gtttttcatc agtccccatc tgccgccggt ggttcagggc gtcgatctga tcgtggttac
     5041 gacgccggcg gttgggcatg agtacttggc gaaggggatt gccgagtatt tatcggacgg
     5101 ccaaaagatt ctgctcaatc ccggccatac cggcggcgcg ctccacttcg cgcacgcgtt
     5161 gcgccaggct ggatgcagag ctgatatcca gctttgcgag acggtgacgc tgacctacat
     5221 ttgccggatg ccgcaatttg gccgggtgga agtgtaccgg cgcacgacca atttgcgctg
     5281 cgcagcgttt cccggcaaat atacggcaac cctaatcccg gagattcaac gcgtttttcc
     5341 caatgtcgtg ccggctgcga acgttatgga aaccggcttc gcgaacatca acgcgatcat
     5401 gcatccggcg ggcatgct
//
LOCUS       DATLCH010000219         4316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5061721, whole genome shotgun sequence.
ACCESSION   DATLCH010000219 DATLCH010000000
VERSION     DATLCH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5061721"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1709)
                     /gene="groL"
                     /locus_tag="VMZ02_06470"
     CDS             complement(45..1709)
                     /gene="groL"
                     /locus_tag="VMZ02_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055846.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HUR71610.1"
                     /translation="MAAKIVKFNRDARDAILRGVNILADAVTVTLGPKGRNVVLDKSF
                     GAPNVTKDGVTVAKEVELEDKFENMGAQMVKEVASKTSDVAGDGTTTATVLARQIFAE
                     GVKLVAAGHDPMTLKRGIDKAVSAIIGELKILSKPTKDPKEIAQVGTIAANNDPTIGA
                     VISEAMNKVGKEGVITVEEAKGLETTLNVVEGMQFDRGYLSPYFVTDPERMECVLEDA
                     YLLIHEKKISTMKDILPILEQIAKSGKPFIIIAEDIEGEALATLVVNKIRGTLRCVAV
                     KAPGFGDRRKAMLEDIAILTGGKLIAEELGIKIDNIALTDLGRAKRLVVDKDNTTIVD
                     GAGKKADLEGRIKQIRAQIDETTSDYDREKLQERLAKLIGGVAVINVGAATEIEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRSSAALDKLRVSEEEKWGVNIIKRVCEEPL
                     RRIAINAGVEGSIALQKVKEGKGAFGFNAASEEYEDLLKAGVIDSAKVVRTALQNAAS
                     VASMLLTTEAMVAEKPEEKSAMPPMPGGGMGGMGGMGGMGGMGGMM"
     gene            complement(1738..2028)
                     /gene="groES"
                     /locus_tag="VMZ02_06475"
     CDS             complement(1738..2028)
                     /gene="groES"
                     /locus_tag="VMZ02_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HUR71611.1"
                     /translation="MKIRPLQDRVIVKRIEEEEKSKGGIIIPDTAKEKPQEGKVLAVG
                     KGKVNDDGKITPLDVKVNDRILFGKYSGTEINVDGEEHLIMREDDILGVIEK"
     gene            complement(2213..2488)
                     /locus_tag="VMZ02_06480"
     CDS             complement(2213..2488)
                     /locus_tag="VMZ02_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HUR71612.1"
                     /translation="MSRVSESRISHLAHLVLDSLTKSKLAEFPADGRSLVETKQVLQE
                     FFRFEDQIDEAVRQKIATLSRHIPPGSREWDVIYRKYYEEEFRKRRR"
     gene            complement(2485..2760)
                     /locus_tag="VMZ02_06485"
     CDS             complement(2485..2760)
                     /locus_tag="VMZ02_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HUR71613.1"
                     /translation="MKIKPEQLDRLATQIVAAYRKKDLIISKATDAELKAKVVETITR
                     NFAEEEVIEEEARKMLASFAQVSRDMDPFKMFLLAKQKLAAKKGFIL"
     gene            3035..3943
                     /locus_tag="VMZ02_06490"
     CDS             3035..3943
                     /locus_tag="VMZ02_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HUR71614.1"
                     /translation="MAVHKPSRLVAGARIGVIAPAGCVDRESLLAGVAAIETQGYAVE
                     LAQGVFDSKGYLAGEAAGRARDLASFFQRGDIAAIFCARGGFGSIQTLDFLSDDIKKH
                     PKIFVGYSDITILLDWLGTRCGMVTFHGPMVAMDFARRLSNRSSEHFWGMLSGRNRTW
                     KLPLGEIIRGGCVEAEMTGGCLSMLVTTLGTPYEIDTRGRILFLEDIGERPYRVERML
                     TQLKMAGKLDHLAGVVFGDFTNCHGDGPRDVASVLGDLFAKAPYPVVMGLPAGHGEEN
                     LALPFGVKMRLDGQGATLEMLEPPVL"
     gene            4014..>4316
                     /locus_tag="VMZ02_06495"
     CDS             4014..>4316
                     /locus_tag="VMZ02_06495"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HUR71615.1"
                     /translation="MNFQTVENAFNEAVAQGVFPGAVVLVCKDEQIVFEKAFGYRSLL
                     PQKSPMQIDTIFDLASLTKPMATTVGIMLLVREKKLRTDDKLTRIVPMFGVFGKSAT"
BASE COUNT          855 a         1130 c         1171 g         1160 t
ORIGIN      
        1 ggtcagtcga ctcgcgtaac gggctgggca actcaaatcc cgagttacat catgccgccc
       61 attccaccca tgccgcccat tccacccatg ccgcccattc caccaccggg catcggaggc
      121 attgctgact tttcctccgg cttttccgca accatggctt cggtggttag taacatactg
      181 gcgaccgacg cggcattctg caacgccgta cggacgacct tcgccgaatc aatgacaccg
      241 gccttgagca ggtcttcgta ctcttccgat gcggcgttga agccgaatgc gcctttgcct
      301 tctttgactt tttgtaacgc aatcgaacct tcaacaccgg cattgatggc aatgcggcgg
      361 agcggttctt cacagacgcg tttgatgatg ttgacgcccc acttctcttc ttctgagacg
      421 cgcagcttgt cgagcgcggc tgaagagcgt agcagtgcaa ctccgccgcc aggcacgatg
      481 ccttcttcca ccgcagcgcg ggtcgcgtgc aaggcgtctt ccacgcgggc ctttttttct
      541 ttcatctcga tttcggtagc tgcgccgacg ttgatcacgg ccacgccgcc gatcaactta
      601 gctaggcgct cttggagctt ttcgcggtca taatcggatg tggtctcgtc gatctgagca
      661 cgaatctgct tgatccggcc ttccagatcg gcctttttgc cggcgccatc gacgatggtc
      721 gtattatctt tgtcgacgac gagccgttta gcgcggccca aatcggtcaa ggcgatgttg
      781 tcgattttta tgccgagctc ttcggcgatc agtttgccgc cggttagaat ggcaatatct
      841 tcgagcatcg ctttgcggcg gtcgccgaat cccggggcct taaccgcgac gcaacgcaac
      901 gtaccacgga tcttgttgac gaccaaagtc gccagggctt cgccttcaat gtcttcggcg
      961 atgatgataa aaggcttgcc ggatttcgcg atttgctcaa ggattggcag aatatctttc
     1021 atggtgctga tcttcttttc gtggatcagc aagtatgcat cctcgagcac gcattccatt
     1081 ctttccggat cggtaacgaa gtacggggag aggtagccac ggtcgaactg catgccctcg
     1141 acgacgttga gggttgtttc taagcctttt gcctcttcga cggtgatgac accttccttg
     1201 cccaccttgt tcattgcttc ggagatgacc gcaccgatgg tgggatcgtt attggcggcg
     1261 atagtgccga cctgggcgat ctctttgggg tccttggtgg gctttgacag aatcttgagt
     1321 tcgccaatta tcgccgagac tgccttgtcg ataccacgct tgagggtcat gggatcgtgg
     1381 ccagcggcta ccaatttgac gccttcggca aaaatttgtc gggccagtac ggtcgcggtc
     1441 gtggtgccgt caccggcgac gtccgaagtc ttgctggcga cctctttaac catctgcgcg
     1501 cccatgtttt caaacttatc ttcgagctcg acttccttgg cgacggtcac gccgtctttg
     1561 gtgacattgg gagcaccgaa ggatttatcc aatacgacat tgcgaccctt gggaccaagg
     1621 gtcacggtaa ctgcgtcggc gagaatgttc acgcctctca ggatcgcgtc acgcgcgtcg
     1681 cgattaaatt tgactatttt agctgccatc gtttcctcct cagattatct gcttaattta
     1741 cttttcgata acgccgagga tgtcgtcctc gcgcatgatc agatgctctt cgccgtcaac
     1801 attgatttcc gtgccggagt attttccgaa taggatgcgg tcattcactt tgacatcgag
     1861 aggggtaatc ttaccgtcgt cgtttacttt accttttccg acggcgagca ctttcccttc
     1921 ctgcggcttc tctttcgccg tatcggggat gatgattcca cccttacttt tctcctcctc
     1981 ctcgatccgt tttacaatga cccggtcctg caaaggccta attttcactt ctcgcctcct
     2041 ttcaagtgtt tgaatttaac tgcctgttta ttaagtagtt tgctgctaat cggagcggcc
     2101 gaccgcgaag ccggcgacaa actagagcgg ggtgaatcta agcatcaatc gggcgctgtc
     2161 aaggtttttg tggcaaattt cttaaagaaa tcgtgcagtc ggcgacatct ccctaacggc
     2221 gacgcttgcg aaattcttct tcgtagtatt tgcggtaaat gacatcccat tcccggctac
     2281 cgggcggtat gtggcgggat agcgtagcga ttttttgacg taccgcctcg tcgatctggt
     2341 cttcaaaacg aaagaattcc tgcaagactt gtttagtttc gaccaaggaa cggccgtcgg
     2401 cagggaactc cgcgagcttt gacttcgtga gactatcaag caccaaatga gctaagtggg
     2461 aaatccggct ctccgagacc cggctcatag aatgaagcct ttcttcgcgg ccagtttctg
     2521 cttggcaagt agaaacatct tgaatggatc catatctctg ctgacctggg cgaaactggc
     2581 gagcatcttg cgcgcctctt cctcgatgac ctcctcctcg gcaaagttac gggtgatcgt
     2641 ttctacaact ttcgctttga gttcggcgtc ggtggctttg ctgattatca gatccttttt
     2701 ccgatatgcg gcgacgatct gtgttgctag tcgatcgagc tgttcgggct ttattttcat
     2761 ggtctgtgaa taagtctgtg gataaatcgt tagccatctg tgggctaaga ttttcttcac
     2821 atcgcgcttt aaggtttagc gagcttgcga tttgaagtca aattagattg gcaatgcttt
     2881 catcggtgtg agaattacat aaattcttgt acatcagggt gttttttgag aagatcgcca
     2941 atcaatggca attagattag ctcgccgcga gattatccac aggtgcaaat tcatttcgcg
     3001 atgattacca gcttttggct tgtggaaaac tgctatggct gtgcataaac catcgcggct
     3061 cgtagccggg gcgcgcatcg gcgtcattgc gccggccggt tgcgttgacc gtgaatcgct
     3121 tttggctggg gttgccgcga tcgagactca aggctacgcc gtggaactcg cccagggagt
     3181 ttttgatagc aaaggatacc ttgccggcga ggccgccggc cgggcccgtg atttggcgag
     3241 ttttttccag cgtggcgaca ttgcggcgat tttctgcgct cgcggcggct tcggatcgat
     3301 tcagactctc gatttcttga gcgatgacat aaaaaaacat ccgaaaatat ttgtcggcta
     3361 cagcgatatc actatactgc tcgattggtt gggcacccgc tgtggcatgg tgacgttcca
     3421 tggtcccatg gtcgcgatgg actttgcgcg ccggctatcg aatcgcagca gcgaacattt
     3481 ttggggtatg ttgagcgggc gtaatcggac ttggaagctg ccgttgggcg aaattattcg
     3541 cggcggctgt gtcgaggctg aaatgaccgg cggctgttta tcgatgctgg tcaccaccct
     3601 aggaacgccc tacgagatcg atacccgagg tcgtattctc tttctagagg acattggcga
     3661 aaggccttat cgcgtcgaac ggatgctgac ccaacttaag atggcgggta agcttgacca
     3721 tctggcgggt gtagtgtttg gtgatttcac gaattgccac ggtgacggac cgcgcgatgt
     3781 ggcgtcggtg ctcggggatc tatttgctaa agcaccttat ccggtggtga tggggctacc
     3841 agcgggtcat ggcgaggaga acttggcgtt gccatttggc gtcaaaatgc gtttggacgg
     3901 ccaaggcgcg acattagaaa tgttggaacc gccagtcttg taggttactt cgcggctgac
     3961 tatgcagcgc aactcggcgt tgcgtgatac ccacaagtta gtattccatg ggcatgaatt
     4021 ttcaaacagt cgagaacgct tttaacgaag cggtcgctca aggggttttt ccgggtgcgg
     4081 tggtgctggt gtgcaaggac gagcagatcg ttttcgagaa agcgttcggc tatcgttcct
     4141 tgttgccgca aaaaagtcct atgcagatcg atactatctt tgatctcgca tcgctgacaa
     4201 aacccatggc taccacggta gggataatgt tgctcgtgcg cgaaaagaag ttgcgcacgg
     4261 acgataagct cacccgtatc gttcccatgt tcggcgtttt cggcaaaagc gcgacc
//
LOCUS       DATLCH010000220         5426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2829213, whole genome shotgun sequence.
ACCESSION   DATLCH010000220 DATLCH010000000
VERSION     DATLCH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829213"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VMZ02_06500"
     CDS             complement(<1..1741)
                     /locus_tag="VMZ02_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR71616.1"
                     /translation="MITFWKLWVWRMAYRDSRRGFLRLLLSMSCVVLAVALVVLAFSF
                     RENLLASIQSQSKSLLGADLAIDSREPFSKDDENLVHSLGGDQARQVGFTSMVTFSAN
                     GDSRLVQVRAIGGGFPYYGALETVPPLSLDSFHSGANALVDENVLLQFNAKVGDGIKL
                     GDQEFRIIGGLRKIPGESLAFSLISPRVYIPLAYLDRTKLIQKGSVVRYRVFFKFAAE
                     TQVDRIVKEIAPQLQRLHLEADTVSRRTASIAANMENLSRFLRLAVFVAVLLAGIGVA
                     SVIYVYAKEKAQAAALLRCIGARPAETVSIYLLQLLFLTIICSAAGVGLGVWAQSYLP
                     AVLQDFLPVAPVIEISPLGIVAGVVIGMTTAFLFALLPLISLRRISPLMALRASYDEE
                     PRAKDPLVVFIVSLIALLVLAFASATLGNWRQGAVFAGAVLLVFILLAILARIGALLM
                     RHLAPRFLAFSWRQGLANLHRPSNQTTSVMLAIGLGTFLLVALHGAQDMLLRQVDRRL
                     GQNEPNLVLFDVQNDQRKAIGELLSRFEISRHAEVPIVTMRLAAVKGRRVEAIRADES
                     AKIPGWALRREYRS"
     gene            complement(1750..2460)
                     /locus_tag="VMZ02_06505"
     CDS             complement(1750..2460)
                     /locus_tag="VMZ02_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR71617.1"
                     /translation="MSTDNILTVEHLNKEFKSGDDTVHVLTDVSFAIEGGTSCAVIGP
                     SGSGKTTLIAICAGLERPSQGAVEFDGVALHKASEEKLARIRNQSIGFIFQSFQLLPS
                     LTALENVMVPAEISGERMVRASALELLGRVGLRNRMYHYPMQLSGGEQQRVAIARAFM
                     NQPKILFADEPTGNLDAETAESIVKLIFNLNAMNRTTLILVTHDRELTEQADRVITLK
                     AGRLASDVTKAGPSRSST"
     gene            2618..3208
                     /locus_tag="VMZ02_06510"
     CDS             2618..3208
                     /locus_tag="VMZ02_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HUR71618.1"
                     /translation="MLTGAAPGPKNILFLGDSITAGYGLEPSQSYPALIQEKIDEKKW
                     PFRAVNAGQSGDTSAGGLNRMEWLLRSPVDVLVLELGGNDALRGLPPETTRQNLQAII
                     ELTRKKYPQAKIILAGMKVPPNMGAGYAGKFNSIYPELAKKHNALLIPFVLDNVGGIR
                     ELNLPDGIHPTAKGHEIVAANVWKVLEPVLQSLHGK"
     gene            3393..3683
                     /locus_tag="VMZ02_06515"
     CDS             3393..3683
                     /locus_tag="VMZ02_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HUR71619.1"
                     /translation="MAVRLIVTITAAPGKGAQLGALYKSRCEDIVKEPGCEQFEVFQS
                     VVNPDHLVILERWTDQAALDVHSKVNATRAPIPGELRVGITEREDYTYNRTR"
     gene            3755..4606
                     /locus_tag="VMZ02_06520"
     CDS             3755..4606
                     /locus_tag="VMZ02_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HUR71620.1"
                     /translation="MAESLESFKSFIGKSESATDVVTASTMVKFAATLGLENPPLEKG
                     SAIPPGWYGGLFPASHRPDKMRTDGQASGGGFMPAIPLPRRRIGGTRVSFHEPLRIGD
                     EVTRKTEIADLKIEDGGEQVTIIERNSMSTARGLCVVEERDMVMLSEARGDAAPKTSP
                     AIPSSAKWNQTVEPNPPLLFRFSAIRFNSHRIHYDRDYVTKIEKLPGLVVQSSLISQL
                     LMEMCRREMPAKRMTNFDFQNLRSVYDSDGKFTLNGNPSADGRDATLWAVDGKGNPAM
                     LVTAKFA"
     gene            complement(4875..>5426)
                     /locus_tag="VMZ02_06525"
     CDS             complement(4875..>5426)
                     /locus_tag="VMZ02_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUR71621.1"
                     /translation="IGGQIVQAMAIERPEMVASLTISTTGPGVRRLDGTLREVGPEAL
                     DEIHKIGFEKYILEHIDNDQMAYNPEFYRSHRQQAAELARALWSGQSTVEQYRAHELA
                     RLTWDTLAEAPKVKVPTLILCGADDGVERRGSTPEATARRLAPLIPGAELALIPGVRH
                     MTFWDGDGALKTLQEFIGRHPIG"
BASE COUNT         1335 a         1573 c         1437 g         1081 t
ORIGIN      
        1 tcgaacgata ctcgcgtctc aacgcccagc cgggaatttt tgcgctttcg tcggcgcgga
       61 ttgcttcgac gcggcgccct ttgacggcgg ccaggcgcat ggtgacgatc ggcacttccg
      121 catgacgcga aatttcgaag cgggacagca attctccaat agctttacgt tgatcgttct
      181 gcacatcgaa tagcactaga ttgggctcat tttggccaag gcgccgatca acctgacgca
      241 acagcatatc ttgcgcacca tgcaaagcca ccaagagaaa tgtcccaaga ccgatcgcca
      301 acatcaccga tgtggtttgg ttgctcggac gatgtaaatt ggccagcccc tggcgccaag
      361 aaaaagccag aaagcgcggc gccaaatggc gcatgagcag cgcgccaatg cgcgccagta
      421 tggcgagtag aataaacacc aaaagcactg cgccggcaaa taccgcgccc tgtcgccagt
      481 tgccgagagt cgcactcgcg aaagccaata ccagtagagc gatcaaagac acgatgaaaa
      541 ccaccagcgg atccttggcg cgcggctctt catcgtagga ggcccgcaac gccataagcg
      601 gcgagatgcg acgcaaagat atcaaaggta agagggcgaa caggaatgcc gttgtcatac
      661 cgatgacaac gccggcaacg attcccagcg gtgagatctc gatcaccggt gccaccggga
      721 gaaaatcctg cagcactgcc gggagataag actgcgccca gacccctaag cccactcccg
      781 cggctgagca aatgatggtt aaaaaaagta gttgcaacag ataaatcgaa acggtttccg
      841 ccggccgggc gccaatacaa cgtaacaatg ccgcagcttg cgccttttcc ttcgcgtaaa
      901 cgtaaataac gctggcgaca ccgataccgg caagcagcac cgcgacaaac accgccaagc
      961 gcaaaaagcg cgaaagattc tccatgttgg cagcgatcga tgcggtgcgt ctgctgaccg
     1021 tatcggcctc cagatgcaag cgttgcagtt gcggcgcgat ctctttgacg atgcgatcga
     1081 cctgggtttc ggcggcaaac ttaaagaaga cgcggtagcg caccacactt cccttttgaa
     1141 tcagcttggt gcgatcaaga tacgcaagcg gaatgtaaac tctagggcta atcagcgaga
     1201 acgccaaaga ctcgcccgga atcttgcgca aaccgccaat aatgcgaaac tcctgatcac
     1261 cgagtttgat accgtctcca accttggcat taaactgcaa caggacattc tcgtccacta
     1321 acgcattggc gccggaatga aacgagtcga gcgatagcgg cggcacggtc tccaaagcac
     1381 catagtaagg aaatcccccg ccgatggccc gtacctgcac cagacgcgag tcgccattgg
     1441 ccgaaaacgt gaccatcgag gtaaagccga cttggcgcgc ctgatcgccg ccgagagagt
     1501 gaaccaagtt ttcatcgtcc ttggaaaaag gttcgcggct atcgatagcc agatcggcac
     1561 ccaaaagact ctttgactgt gattgaatgc tggcgagcaa gttctcgcga aacgaaaacg
     1621 ccaagactac caatgccacg gccagaacca cacacgacat cgacagtaac aagcgcaaga
     1681 aaccgcgccg gctgtcgcga taggccatgc gccacaccca gagtttccag aacgtaatca
     1741 tctatatgct caagtcgagc tgcgcgaagg cccggccttg gttacgtcgg acgccaagcg
     1801 cccagccttg agagtgatca cacggtcggc ttgttcggtg agctcgcgat catgggtaac
     1861 cagaatcagc gtcgttcgat tcatggcgtt caaattgaat attagtttaa caatactctc
     1921 ggcggtctcg gcatcgagat ttccggtggg ctcatcggca aacaagatct tcggctgatt
     1981 cataaatgcc ctggcgatcg caacgcgctg ctgctcgcca ccggaaagtt gcatggggta
     2041 atgatacatg cgattacgca ggccgactcg gccgagcagc tccaaggcac tggcgcgcac
     2101 catgcgctcg ccgctgatct cggcgggcac catgacgttt tccagagccg ttagtgacgg
     2161 cagcagttga aaactttgga agataaaacc gatggactgg ttacgaatac gcgctaattt
     2221 ctcttcgcta gccttatgca gagcgacacc gtcgaactcc accgcgccct ggctgggccg
     2281 ctccagaccg gcgcagatgg cgatcaaagt agttttgccg ctgcccgaag gtccaatgac
     2341 agcacagctt gtgccgcctt cgatcgcaaa ggacacatcg gtcaagacat gaacggtgtc
     2401 gtcgccactc ttaaattcct tatttaaatg ctctactgtt aaaatgttat cggtgctcat
     2461 gcgagatgca gttacacaga aattaggatt aaaacgcttc ccatcaaatc gactgtaaat
     2521 caaatacagc aaatctagaa cttataagat gacgacacaa tacagttttc cacgattgaa
     2581 cttgtggcgg tacggactat atcttagttt ttttttaatg ctcactggcg cggcacccgg
     2641 accaaagaat attctgttcc tcggcgacag catcaccgcc ggttacggcc tcgaaccctc
     2701 tcagtcctat cccgctctaa tccaggaaaa gatcgatgaa aaaaagtggc cgtttcgagc
     2761 cgttaatgcc ggccagagcg gcgacactag tgctggcggg ttgaaccgaa tggaatggct
     2821 tctacgctct ccagtagacg tcctggtttt agaactcgga gggaacgacg ccctgcgcgg
     2881 ccttcctccg gaaacgaccc gccagaacct tcaagcgatc attgaactca cgcgaaaaaa
     2941 atacccgcag gctaaaatca tattggccgg catgaaagtg ccgcctaata tgggcgcggg
     3001 ttacgctgga aaattcaact cgatctaccc tgaactggcg aaaaagcaca atgctctgct
     3061 catccctttt gttcttgata acgtcggcgg cattcgcgaa ttgaatttgc ccgacggcat
     3121 tcacccaacc gccaagggtc atgaaattgt cgctgccaat gtttggaaag ttttggagcc
     3181 ggttctgcaa agtttgcacg gcaagtaatg ccagtttgaa aacattcgaa cctcgcgaat
     3241 gattcccgcc tcaggatgtc aagtcctata agggtcgcca gctgaatcgt catatacttc
     3301 gttacttgtc ttgacaccat caccttacct ggacgatagt ttccgctatc acgatcaaca
     3361 acgcgcccag acacagcgcg acaaggagac ccatggcagt tcgactgatt gtcacaatca
     3421 cagccgcgcc cggcaaaggc gcgcaactgg gcgcactata caaatcccgc tgcgaagaca
     3481 ttgtcaaaga acccggttgc gaacagttcg aagttttcca aagtgtcgtc aaccccgacc
     3541 acctggtcat tctcgaacgc tggaccgacc aggcggcttt ggacgtgcac tcgaaggtta
     3601 atgcaacgcg cgcgcctatc cctggagagc tgcgcgtcgg cataaccgaa cgcgaggatt
     3661 acacgtacaa ccggacacgc tagcgtctcg cgaaacggca attatcgtaa caggcgcaga
     3721 attcgctgcg ctacaatcaa tcccgaggtg aacaatggcg gaatcacttg aaagctttaa
     3781 aagtttcatc ggcaagagcg agagcgcgac ggatgttgtc acggcttcaa cgatggtgaa
     3841 attcgccgca acgttgggac tggagaaccc gccgttggaa aaaggctcgg caatcccgcc
     3901 cggatggtac ggggggttat ttccggcgtc gcaccggccg gacaagatgc gcaccgacgg
     3961 acaagcgtcg ggcggcggct tcatgccggc gatcccgctg ccgcgccgaa gaatcggcgg
     4021 gacgcgcgtg tcgtttcatg aacccctgcg aatcggtgat gaggttacgc gcaaaaccga
     4081 gatcgctgac ctgaagattg aggatggcgg tgaacaagtg accatcatcg agcgcaacag
     4141 catgtccacc gcgcgcggct tatgcgtcgt tgaagaacgc gacatggtca tgttgagcga
     4201 agcgcgcggc gatgctgcac ccaaaacctc gccagccatt ccaagctcag cgaaatggaa
     4261 tcaaacggtg gaacccaatc cgccgctgct gttccgcttc tcggcgattc gttttaacag
     4321 ccaccgcatt cactacgatc gcgattacgt caccaagatc gaaaaactgc cggggctggt
     4381 ggtgcaaagt tcactgatct cccagctgct gatggaaatg tgccgaaggg agatgccggc
     4441 caagcgcatg accaacttcg attttcaaaa cctccgctca gtctacgaca gcgacggtaa
     4501 gtttactctg aacggcaatc cgagcgccga cgggcgggac gccacgctgt gggcggtgga
     4561 tgggaaaggc aatccggcaa tgttagtaac cgcgaaattc gcctaaaagc gaccgctcga
     4621 aagagggtat gggcgcagca tgctgcgccc ttttttttcc gcccttgtgc tctgcttcga
     4681 agatacgtcc aggaaaatct tggcgccgaa tcggatcgag gttccgtcat accggcgaaa
     4741 gccggtatcc agcccctcct cgcgtcgatg ccggcctgcg cgtaggaaca ccaggtggag
     4801 caggttgcga gttcatcctt tgttgacgag cgtaagctaa tcaacacttt gtggttaaat
     4861 ctccgaatct atgtctaccc aatcggatgg cgcccaatga actcctgcaa agttttcagc
     4921 gcgccatcac catcccagaa ggtcatatgc cttacgcccg ggattagcgc caattcggcg
     4981 cctgggatta gcggcgcaag tcgccgcgcc gtcgcttcgg gcgtgctgcc gcgccgttcg
     5041 acaccgtcgt cggcgccgca cagaatcagc gtcggcactt ttaccttggg cgcttccgcc
     5101 agcgtgtccc aggtaagccg cgccaattcg tgagcgcgat actgctccac cgtgctctgt
     5161 cccgaccata gcgcccgcgc caactctgcc gcctgctgcc gatgactgcg gtagaattcg
     5221 gggttatacg ccatctggtc gttgtcgata tgttcgagga tatatttctc gaaaccaatc
     5281 ttgtgaattt catccaacgc ttccggtccc acctcgcgca gcgtgccatc gaggcggcgc
     5341 acgcccggtc cggtggtgga tatcgttaga ctcgcgacca tctccggccg ctcgatcgcc
     5401 atcgcctgaa cgatctgccc tccaat
//
LOCUS       DATLCH010000221         5862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2832118, whole genome shotgun sequence.
ACCESSION   DATLCH010000221 DATLCH010000000
VERSION     DATLCH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2832118"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VMZ02_06530"
     CDS             complement(<1..334)
                     /locus_tag="VMZ02_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HUR71622.1"
                     /translation="MKLLIIGSGGREHALVWKLSQSPRVTKLFCAPGSAAIGELAECV
                     AINPEQLDRLAEFADKEKIDLTVVGPELPLTLGIVDLFESRGLKIFGPNKAAAQLEGS
                     KAFTKQILQ"
     gene            complement(405..1979)
                     /gene="purH"
                     /locus_tag="VMZ02_06535"
     CDS             complement(405..1979)
                     /gene="purH"
                     /locus_tag="VMZ02_06535"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HUR71623.1"
                     /translation="MGKIQRALISVSDKNGIVEFSRALVAMGVELVSTGGTAALLRDN
                     KIPVKDVSELTDFPEMMDGRVKTLHPKIHGGILALRDNPEHVAKMREHGIQPIDLVVV
                     NLYPFEATVARGAAFEEVVENIDIGGPSRVRAAAKNHQHVGVVVDPADYDTIISELKS
                     NHGALLPATHFRLFCKAFQHTAHYDGAISNYFSSLDEEKKPQRWGQTVNIQVNKLQDM
                     RYGENPHQSAAFYGTAGDAGPAISRAKQFQGKELSFNNILDADAALSTVLEFSDIATV
                     AIKHNNPCGVALSTKSLADSFRKAKACDPVSIFGGVLAFNRPVDEETALELKEIFLEI
                     VIAPSFTPQAKAVLSSQKRLLNIRLLECDMSEPQTGGYDLRRVRGGMLIQDWDTGKID
                     VRACKVVTERKPTDDEYRALDFAWRVCRHVKSNTIVFASPDQVLGVGAGQMSRIDSTK
                     IAILRAATHGLDLKGSAVASDAFYPFRDGLDEAAKGGAKSIIQPGGSIKDDEVIAAAN
                     EHGIAMIFTGMRHFRH"
     gene            complement(1993..3126)
                     /gene="alr"
                     /locus_tag="VMZ02_06540"
     CDS             complement(1993..3126)
                     /gene="alr"
                     /locus_tag="VMZ02_06540"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HUR71624.1"
                     /translation="MTNTPPGRPTLCFIDHQALRWNFKQIRTLVGPRVKVLSMVKANA
                     YGHGAQAVAKTLAAAGSDAFGVATVEEGSELRRARITQPILVVAGFYVEQLEQFIKNR
                     LTPVVHEVATLKELDKQLQRLKTTLGVEIEVDTGMGRIGFPAAEIRAWLPALAALKAI
                     KLTGVFSHFSDAERADERVTQKQLSSFHEVLNRLAEAKMTHRSSHMAKSAALITEPAA
                     HFSMVRPGLILYGMYPAPALQENIDLKPVLSWKTRIIQLKRVEAGTSVGYGRTFTAKR
                     NSLIATLPVGYADGYRRLFSNRAHVLVRGKRAPVIGRVSMDLTTIDVTDIRNIQQGDE
                     VVLLGIQGAAEIGADEIAGWANTISYEIFTSISARVPRIHYHN"
     gene            complement(3156..3500)
                     /gene="metG"
                     /locus_tag="VMZ02_06545"
     CDS             complement(3156..3500)
                     /gene="metG"
                     /locus_tag="VMZ02_06545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HUR71625.1"
                     /translation="METSEISTTISIEDFRKIELKVATIKAAEPHPNADKLMVLQIDL
                     GTEQRQICAGIRSSYTSEELVGKQIVVVTNLATARLRGMDSQGMLLAASSGDRVIVMT
                     PDQPALAGSIVK"
     gene            3609..4034
                     /locus_tag="VMZ02_06550"
     CDS             3609..4034
                     /locus_tag="VMZ02_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HUR71626.1"
                     /translation="MAKSLSGTKSHENLKGAFAGESQANRRYLYFARVADIEGYPDVG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPCTGVPIGTTAKNLKSAVEGETYEYTEMYPGF
                     AKTARDEGLPELAEWFETLAKAEKSHAGRFAKGLDTLGS"
     gene            4111..4692
                     /locus_tag="VMZ02_06555"
     CDS             4111..4692
                     /locus_tag="VMZ02_06555"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HUR71627.1"
                     /translation="MKKIALDDILGFAAYEQARDEFRREIIEKKKLRRVAVGENISLV
                     FENRDTVIFQIQEMLRAERIVDLDKIREEISVYNELVPNPGELSATLFIEIEDQNHLR
                     DELLKFRGIEESLLMTVGDHRIPGRFEAGRSKEDKISAVQYVRFLFTGEARQALIAGV
                     PAEVVIDHPNYRVSKVLGAAVQESLARDLADSL"
     gene            4689..5081
                     /locus_tag="VMZ02_06560"
     CDS             4689..5081
                     /locus_tag="VMZ02_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HUR71628.1"
                     /translation="MTSMRTSARKTKQLSLVWQAIKDDKSHPTADQIYARVRRKLPNI
                     SLGTVYRNLQKLVGSKKLQVLMLGRSQHFDPLVQRHQHFICEVCDRVFDVLVDAQREI
                     RPAKLPEEGFTVTSHQLAFYGTCKTCSS"
     gene            5185..>5862
                     /locus_tag="VMZ02_06565"
     CDS             5185..>5862
                     /locus_tag="VMZ02_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR71629.1"
                     /translation="MKRRGFIGALTSFLLLPLREMVEAVCNPSVVHFFHHGRQLVQTR
                     GNNGKSLVLGSYFLQWYGHSSFLIHSGSQTKVVADPNFNVTPGIQADAVTISNDHFTH
                     NNTGAVTGNPVILRGITFKQTWQPIRTSIKDITIVNIPSQRSANWGAIANSIFVYEMG
                     SLCLAHLGNIGHLLTEEQAKVLQRIDVMMVPIDAMTNLGFEDVMKVIDQVKPPIVIPM
                     HYDVARQA"
BASE COUNT         1301 a         1569 c         1653 g         1339 t
ORIGIN      
        1 cttggaggat ttgcttggta aaagctttgc tgccttcgag ttgggcggca gctttgtttg
       61 gtccgaaaat cttcagaccg cgcgattcaa agagatcgac gatgccgagg gtcagcggca
      121 attccggtcc gactacggtc aaatcgattt tttctttatc cgcgaactcg gcgagacgat
      181 cgagttgctc tgggttgatg gctacgcatt cggctaattc gccgatggcg gcgctgcccg
      241 gcgcgcagaa gagtttcgtt acgcgcgggc tttggctgag cttccagacc agcgcatgtt
      301 cgcggccgcc gctgccgatg attagcaatt tcatgaagcg tcgaaagttg acgatcgagg
      361 acggagtatc ccaatcatct ataactcacc gccggagagg ctacctaatg tctaaaatgc
      421 ctcatccctg taaaaatcat tgcgatgcca tgttcgttag cggcggcaat gacttcgtcg
      481 tccttgatcg agccgcccgg ttgaatgatc gatttggcgc cgcctttggc agcctcatcc
      541 aagccatcgc gaaatggata aaaagcatcg gaagccaccg ccgatccttt gagatcgagt
      601 ccatgggtgg cggcgcgcag gatggcaatc ttggtcgagt caatgcggct catctggccg
      661 gcgcctacgc cgagcacctg gtccggggaa gcgaaaacaa tggtgttgga ttttacgtga
      721 cggcatacgc gccaggcgaa gtcgagggcg cgatactcgt cgtccgtggg tttacgttcg
      781 gtgacgactt tacaggcgcg cacatcgatt ttgccggtat cccaatcttg aatcaacatg
      841 ccgccacgca cgcggcgaag atcataaccg ccggtctgcg gctcgctcat gtcgcattcg
      901 agcaagcgaa tgttgagcag tcgtttctgc gaagacagca cggccttcgc ttgcggggta
      961 aagctcggcg cgatgacaat ctcgagaaaa atttctttga gctccaaggc ggtctcttcg
     1021 tccaccggtc gattgaaggc caagacgccg ccaaaaatgg aaaccggatc gcaggccttg
     1081 gctttgcgaa acgagtcggc gagggatttt gtcgataggg cgacaccgca aggattgtta
     1141 tgctttatcg ccacggtggc gatatcggag aattctaaca ccgtgctcaa ggcggcatcg
     1201 gcgtcgagga tattgttgaa ggaaagttct ttgccttgga actgtttggc gcgcgagatc
     1261 gctgggccgg cgtcacctgc ggtgccgtaa aacgcggcgc tctgatgggg attctcgccg
     1321 tagcgcatgt cttgcaattt attgacttgg atgtttaccg tttgacccca gcgctgcggc
     1381 ttcttttctt catcgaggga tgaaaaatag ttggaaatgg cgccatcgta atgcgccgtg
     1441 tgctggaatg ccttgcagaa aagccgaaag tgcgtcgctg gcaaaagcgc gccgtggttg
     1501 gatttcaatt cgctgataat agtatcgtaa tctgccgggt cgaccacaac accgacatgc
     1561 tgatggttct tcgctgcggc gcggaccctg ctcgggccgc cgatgtcgat gttttcgacg
     1621 acttcttcga atgcggcgcc acgagccacg gtggcctcga agggatacag attcaccact
     1681 acgagatcga taggctggat gccatgctct cgcatcttgg cgacatgttc aggattgtcg
     1741 cgcagcgcca gaatgccgcc gtgaattttt ggatgcaggg ttttgacccg accatccatc
     1801 atttccggga agtcggtgag ctcagaaaca tctttgacgg gaatcttatt gtcgcgcagc
     1861 aacgctgcgg tccccccggt ggagacaagc tcgactccca tcgcgacgag cgcgcgggag
     1921 aattcgacga tgccgttttt gtcagaaacg ctaatgaggg ctctttgaat cttgcccact
     1981 gcaggattcc tcctaattgt ggtagtggat gcgcggaacg cgagcgctga tggaagtgaa
     2041 aatttcgtat gagatcgtgt ttgcccaacc ggcgatttcg tcggcgccga tttccgcagc
     2101 gccctgaatg ccgaggagaa caacctcgtc tccctgctga atatttcgta tatcggtaac
     2161 atcgatggta gtcaaatcca tgctcactct gccgatcacc ggcgcgcgtt tgccgcgcac
     2221 caggacatgc gcgcgattgg aaaaaagccg gcggtaaccg tcggcgtagc ccaccggcag
     2281 cgtggcgatc aggctgttcc gtttcgccgt gaatgtgcgg ccatagccaa cgctggttcc
     2341 ggcctcgacg cgcttcaatt gaatgatccg ggttttccaa cttagcaccg gcttgagatc
     2401 gatgttctct tgcagcgccg gggcaggata catcccgtag agaatcagcc cgggacgcac
     2461 catagaaaaa tgcgctgccg gttcggtgat gagggcggcg cttttagcca tgtggctgct
     2521 ccgatgggtc attttcgcct cggctaagcg gtttaaaact tcgtgaaaac tgctcagttg
     2581 tttttgcgtg acgcgctcgt cggcgcgctc ggcatcggaa aaatgcgaaa acactccagt
     2641 cagctttatc gctttcaggg cggcgagcgc cggcagccag gctctaattt cggctgccgg
     2701 aaacccgata cggcccatgc cggtgtcgac ttcgatttcg acgccaaggg tcgttttcaa
     2761 tcgttgcaac tgcttgtcca attctttcaa agtggcaacc tcatgaacca ccggcgtcag
     2821 ccggttcttt ataaactgtt ccaactgttc gacgtagaac ccggcgacca ccaagatcgg
     2881 ctgtgtaatg cgcgctcgcc tgagctcgct tccttcctcg accgttgcta cgccgaaagc
     2941 atcgctgccg gcggcggcta aagttttggc cacagcctgg gcgccatggc cataagcgtt
     3001 tgctttaacc atggatagga ctttgacccg cggaccgacc agagtgcgaa tctgtttgaa
     3061 gttccagcgc aacgcctggt gatcgatgaa gcagagggtg ggccgccctg gtggggtgtt
     3121 ggtcatgaaa acagcggaac gatccagctg gaggcttact taacgatgga tccggcaagt
     3181 gccggttggt ccggtgtcat aacgatcacg cggtcgccgc tggacgcagc gagcaacatg
     3241 ccttggctat ccatgccgcg caatcttgca gtcgccaaat tggtgaccac aacgatctgt
     3301 ttcccgacca gttcctccga cgtgtaggag cttctgatac cagcgcagat ttgccgttgt
     3361 tccgtgccga gatcgatttg caggaccatg agcttatctg cgttgggatg cggttcggca
     3421 gctttgattg tagctacttt cagctcgatc tttctaaagt cctcgatgga gatggtcgtg
     3481 ctaatttcag aagtttccat tgggcaaaaa tatcagagtt ggagcgctaa aaaaagtaaa
     3541 tcgttggatt gacactccag cactccttcc ataatagtaa cgattatcaa ttaagaggag
     3601 ggagctttat ggcaaagagt ttatctggca cgaagtctca tgaaaacctg aagggcgcct
     3661 tcgcgggcga gtcccaggcg aatcggcgct acctgtattt tgcacgggtc gcggatatcg
     3721 aagggtatcc cgatgtcggt ggcttgtttc gcgacacttc cgaagccgag actggtcacg
     3781 ctttcgggca tctcgacttt ctcaaagaag tgggtgatcc ctgcaccggt gtgccaatcg
     3841 gtacaacggc gaaaaattta aagtccgcgg ttgaaggcga gacttacgaa tacacggaaa
     3901 tgtatccggg cttcgccaag actgcgcgcg acgaggggtt gccagaacta gccgaatggt
     3961 ttgaaacttt ggccaaggca gaaaagtctc atgccggacg gttcgccaag ggcctcgaca
     4021 ccctcggcag ttaatgctgg gctgacccta gtgtgacggc ggcggatagc gccggttatc
     4081 ggctatccgc cgctctagtc cttgcaggtc atgaaaaaaa tcgctctaga cgatatcctg
     4141 ggctttgccg cctacgaaca ggcgcgcgac gagtttcgca gggaaataat tgaaaagaaa
     4201 aagctccgtc gggtggccgt gggcgagaac atttcgctgg tgttcgaaaa tcgtgacacg
     4261 gtgatttttc agatccaaga gatgttgcgc gccgaacgaa tcgtcgacct cgataaaatt
     4321 cgcgaggaga ttagcgtcta caacgaactt gtccccaatc ccggcgagct cagcgccact
     4381 ctattcatcg aaatcgaaga ccagaaccat ttacgcgatg aactgctaaa attccgcggc
     4441 attgaggaat ccctgctgat gacggtcggc gaccacagaa tccctggacg attcgaagcg
     4501 ggacgaagta aagaggataa aataagcgcg gtgcaatatg tgcgctttct attcactggg
     4561 gaagctcgac aagcgctcat cgccggtgtg ccggctgaag ttgtcatcga tcatccgaat
     4621 taccgtgtca gcaaagttct tggcgccgca gtacaggagt ctttggcgcg ggatttggcg
     4681 gattcgctat gacctccatg cggacttcgg cgcgcaagac caaacaatta tcgctggtct
     4741 ggcaagccat caaagatgac aaatcgcatc caaccgcgga tcagatctat gcgcgagtac
     4801 gccgcaagct gcccaacatc agcctcggca cggtgtatcg caatctgcaa aagttagtcg
     4861 gatcaaaaaa gctgcaggta ttgatgctcg gacgctccca acacttcgac ccgctggtgc
     4921 agcgccatca gcacttcatc tgcgaagtgt gcgatcgcgt cttcgatgtg ctggtcgacg
     4981 ctcaacgaga aattcgcccg gcaaagcttc ccgaagaggg cttcaccgtg acatcgcatc
     5041 agttggcctt ctacggcacg tgcaagacct gctcttctta aagtgcgacc gggttgtgga
     5101 tggcctgcgg cgatcgtgtt ctcatccgcg acgatgacta atgcacgagc atttaagatt
     5161 acactgcagc catgaggagg agaaatgaag cgacgtggtt ttattggcgc cctcacgagc
     5221 tttttgctcc tgccgctgcg ggaaatggtc gaagccgtgt gcaatccgtc ggtcgtgcat
     5281 ttctttcatc atgggcgcca gctggttcag acgcgcggca ataacggcaa gagcctggtt
     5341 cttggttctt atttccttca gtggtacggc cattcgagtt ttttaatcca ctcgggcagc
     5401 caaaccaaag tcgtcgccga tcccaacttc aacgtcacgc cgggaatcca agccgatgcg
     5461 gtgaccatta gcaacgatca cttcactcac aataacaccg gtgccgtaac cggcaatccg
     5521 gtgattctcc ggggcattac cttcaagcag acctggcagc ccatccgcac cagcataaaa
     5581 gacatcacca tcgtcaacat tcccagccag cgtagcgcca attggggcgc gatcgctaat
     5641 tcgatcttcg tgtacgagat gggtagccta tgtctcgcgc acctgggcaa catcggtcac
     5701 ctgcttacag aagagcaggc gaaagtgcta cagcggatcg atgttatgat ggtcccgatc
     5761 gacgcgatga ccaatctcgg ttttgaagac gtcatgaaag tcatagatca ggtaaaaccg
     5821 cccatcgtga ttccgatgca ttacgatgtc gcgcgccaag cc
//
LOCUS       DATLCH010000222         2993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4301359, whole genome shotgun sequence.
ACCESSION   DATLCH010000222 DATLCH010000000
VERSION     DATLCH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4301359"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VMZ02_06570"
     CDS             complement(<1..549)
                     /locus_tag="VMZ02_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71630.1"
                     /translation="MKACAIDVHHHYVPESLLEEAKKRGQHLGVTLTEKDGNRSLAFA
                     GGPPFQLHPELPAIDQRLKMMEDSRLAMAALEAHTATLGYRLTGEQGENWCNVYNDGI
                     AELVKKYPDRFVGMASVPLQDPPRAAKVLERAVRDLNFRGGYIGTNVNGNYYNSEEFD
                     PFWAKAQELDVLVVMHPEDVAGS"
     gene            complement(657..2321)
                     /locus_tag="VMZ02_06575"
     CDS             complement(657..2321)
                     /locus_tag="VMZ02_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71631.1"
                     /translation="MAHDVVIKNGNVIDGSGSPAFQADVAIDGDKITAIGKNLGDGKK
                     EIDAKGHVVAPGFVDSHTHMDAFVVQYPNGNPVVNYGVTSIVIGDCGASCAPVPPKPE
                     PRKVLVEYLRRVLDKYVDDKDWRWNTFGEYLNYLEGKIAINCLPLMPHSPVRLTVMGE
                     AAYQRAANPEELNAMKAMVREGMELGAIGFSTSPRGGPAIHAGTPSTFAEHEEIIELA
                     NVAAEYKGCFQFNGFQMVLQPESGVPEMLTKIKTLQIGNEFRVRPGAKAAGMESIRYM
                     EEAQKRGQDIYGVVIPYQHIRRFNLDDCFLFNGLPTWEAIKNSSDLKAKLSDKDTRRK
                     IEQERIACQGKLEFPEWHGWDRVVFEHLEKPELKKLEQKNMAEVARLTEKQPVDAFFD
                     IWIEDNFNSQILYHGLANAHPELLAEMIKSDTSLIGTDVGAHLDRFFWHGAPTKILGY
                     WRREKNLMSLEAAVHKITGYPAKKLRLNRGLVREGLPADITVFDADKIDDLVSKKLPG
                     MVDAQEVQRHPPGMKAVLVNGSTVVEDGHCLDVFPGKVRRQQLCLN"
     gene            complement(2541..>2993)
                     /locus_tag="VMZ02_06580"
     CDS             complement(2541..>2993)
                     /locus_tag="VMZ02_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HUR71632.1"
                     /translation="SRLDVTFSMVWVLILANIIVTALCFLILNHLAALTFIRGSLLIP
                     FLLFLVFIGSFTANNDLADIIVTLLFGVIGYCMVKFGWPRPPLILGLTLGKIAENYLW
                     ISTAAYGAKWLLFPTVLGLIALTLFTIAYPTIKERMGKAKYERPEDAV"
BASE COUNT          637 a          813 c          863 g          680 t
ORIGIN      
        1 agacccggcg acatcttcgg ggtgcatgac caccaagacg tcgagttcct gggctttggc
       61 ccagaacgga tcgaactcct cgctgttgta gtaattgccg ttgacgttgg tgccgatgta
      121 accgccgcgg aagtttaagt cacgcactgc gcgttccaat actttcgcgg cacgcggcgg
      181 atcttgcagc ggcaccgagg ccatgccgac aaagcgatcg gggtacttct ttaccaactc
      241 ggcgatgccg tcgttgtata cgttgcacca gttctcgcct tgctcgcccg taagccgata
      301 tcctagagtc gccgtgtgcg cttccagcgc tgccatggca agccgcgaat cttccatcat
      361 cttgagccgc tgatcgatgg ccggcagttc ggggtgcaat tggaatggcg gtccaccggc
      421 gaaggcgagc gagcgattgc cgtctttttc ggtcagtgtg acaccgaggt gctggccgcg
      481 cttttttgcc tcttcgagta gactctccgg cacataatgg tggtgaacgt cgatggcaca
      541 ggctttcatg gttatcgtca tccttcgttg agataaaatt aaaaatccct caccctaacc
      601 ctctcccatc gcgggagagg gttagttgaa gggacgaaag aactgtcggt tgaagtttag
      661 ttcaaacaga gttgctgacg gcgcactttg ccggggaaga cgtcgagaca atggccgtct
      721 tcgacgaccg tgctgccgtt aaccaggacg gctttcattc cgggcggatg ccgctgcact
      781 tcctgagcgt cgaccatgcc gggaagcttt ttgctgacca gatcgtcgat cttatcggca
      841 tcgaagaccg tgatgtcagc gggcaatcct tcgcgcacta gaccgcggtt gaggcgcaat
      901 ttcttcgccg ggtaaccggt aatcttgtgc acggccgctt ccaaactcat gagatttttc
      961 tcgcggcgcc agtagccgag gatcttggtc ggcgcgccat gccagaagaa gcgatcgaga
     1021 tgagcgccga cgtcggtgcc aatcaagcta gtgtcggact tgatcatctc ggcaagcagc
     1081 tcgggatgcg cattggcgag tccatgatag agaatctgcg agttgaagtt gtcttcgatc
     1141 cagatgtcga agaatgcgtc caccggctgc ttctctgtaa ggcgggcgac ttcggccatg
     1201 ttcttctgct cgagtttctt gagttcgggc ttttccaaat gctcaaacac gacgcggtcc
     1261 cagccatgcc attcgggaaa ctcgagcttg ccctggcagg cgatgcgctc ttgctcgatt
     1321 ttacggcgcg tatctttgtc gctcagcttg gccttgagat cactcgagtt cttaatcgct
     1381 tcccatgtcg gcagaccatt gaataggaaa cagtcgtcca ggttgaagcg acgaatatgc
     1441 tgatagggaa taaccacgcc gtaaatatcc tggccgcgct tctgcgcttc ttccatgtag
     1501 cggatcgact ccatgccggc ggctttggcg cccggacgga cacggaattc gttgccgatt
     1561 tgcagcgttt taatcttggt caacatctcg ggcacgccgg actccggctg caggaccatc
     1621 tgaaagccgt tgaattgaaa gcagcctttg tactccgccg cgacattggc gagctcgatg
     1681 atttcttcat gctcggcaaa agtgctcggt gtgccggcgt ggatcgccgg gccgccgcgt
     1741 gggctggtcg aaaaaccgat ggcgcccaac tccatgcctt cgcgcaccat cgccttcatg
     1801 gcgtttagtt cttcagggtt ggcggcgcgc tggtaggcgg cctcgcccat gacggttagc
     1861 cggaccggag aatggggcat cagcggcaaa cagttgatgg cgattttgcc ctcgagatag
     1921 ttcaaatatt cgccgaaggt attccagcgc caatccttgt cgtcgacata tttatcaagc
     1981 acgcggcgca ggtattcgac caggactttg cgcggctcgg gtttgggcgg caccggcgcg
     2041 caggaagcgc cgcagtcacc gatgacgatg ctggtgacac cgtagttcac caccggattg
     2101 ccgttgggat actgcacgac aaacgcatcc atgtgggtat gcgaatcgac aaatccaggc
     2161 gcaacgacat gtcccttggc gtcgatctct tttttgccat caccgagatt tttaccgatg
     2221 gcggtaattt tgtcgccatc aatggcaaca tccgcttgga aagccggact gccgctaccg
     2281 tcgataacat tgccgttctt aatgactaca tcgtgggcca taattgggtg tcctcccttg
     2341 gatctcttag atgctttgct tatcccactg aaatcagtgg tgtgtcaacc gcttatgtgg
     2401 atttgacggc cggcatcctg cgacagtaat gcaggcgatg aagcgcgctg gaaaacgcct
     2461 ggcgtctcga gtcctgccga actcgagacg ccaatcgcaa acgctaatga atgctaaata
     2521 agatcgcgag atgacgccgg ctaaaccgcg tcttccggac gctcgtactt ggctttgccc
     2581 atgcgctctt taatggttgg gtaggcgatg gtgaagagtg tcagggcgat aaggcccagg
     2641 acggtcggga ataacagcca tttagcgccg tacgcggcgg tcgagatcca cagataattc
     2701 tcggcaattt tcccgagggt caggcctagt attagcggcg gccgcggcca accgaacttg
     2761 accatgcaat aaccgatcac gccaaaaagc aacgtgacga tgatgtcggc aagatcgttg
     2821 ttggcggtga aagatccgat gaaaacaaga aacaagagaa acgggatcag caacgagccg
     2881 cggataaacg tcagcgccgc gaggtggttc aaaatcagga agcacaacgc ggtgacgata
     2941 atgttggcga ggatgagtac ccaaaccatc gagaaggtga catcgagtcg gga
//
LOCUS       DATLCH010000223         5600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4302357, whole genome shotgun sequence.
ACCESSION   DATLCH010000223 DATLCH010000000
VERSION     DATLCH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4302357"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..823)
                     /locus_tag="VMZ02_06585"
     CDS             complement(314..823)
                     /locus_tag="VMZ02_06585"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HUR71633.1"
                     /translation="MDNELRQQVEDFYYLEAELLDERKLRQWFELLTEDIRYWMPLRY
                     NPLERPENITDEVSPPGQAYYFDDDWKSLKIRVERAYSKIAWAEVPPSRTRHLITNVR
                     IKKNDSAEVEAHCNFLVYRTRQESDKDIFVGTRQDILRRAGTTFKIARRTIILDQAVL
                     DAKNISVFL"
     gene            complement(816..2099)
                     /locus_tag="VMZ02_06590"
     CDS             complement(816..2099)
                     /locus_tag="VMZ02_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HUR71634.1"
                     /translation="MDIKSLVDPEGGLIDRRIFADRDIYDLERERIFARCWLYLGHEC
                     EIPNRGDFVTRYMGEDPVILARDLNGELQAHLNMCRHRGNRVCRADRGNTKLFQCSYH
                     GWSYTTDGKLALVPMVDSFGKLDRSQWGLVSVAQVDSYKGLIFATFDKDAPTLREYLG
                     DMAWYLDILLDRREGGTEVSGPQRWVVNANWKTAAENFGGDGYHIASTHGSARELGID
                     TTTSKTRQWNKGCQIACDNGHILVSWLAPPDGGPWFAQPDDELVDYMKEHEAEIEKRL
                     GTKRARQIVPSAGTVFPNLSVHWLTRTIRVWQPRGPDKMEIWSWAIVDKAASPKIKDV
                     MRFVSQYRFSPSGVFEQDDMDNWIQVTGAARSLIGQRFPANYQMSGNEAPIEIDLPGR
                     VKSRFSDNNQLSLYMHWAKMLEAKSWSAVSGAIHG"
     gene            complement(2212..2361)
                     /locus_tag="VMZ02_06595"
     CDS             complement(2212..2361)
                     /locus_tag="VMZ02_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71635.1"
                     /translation="MAVLPGRLIASGQRRGKMFLRLFGRSFAVGMNRFLAVLEMTKTA
                     VVITK"
     gene            2360..3889
                     /locus_tag="VMZ02_06600"
     CDS             2360..3889
                     /locus_tag="VMZ02_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71636.1"
                     /translation="MSTESKITRRRFLQRSSSVGLGALIGLTANRSALAASKERAVIY
                     QGVSLDSLHPYGYSGGGINGIWLHLIEPLIVMDYTRQKYVGMLADSWEFQGRRWIFQL
                     RRNIRFHNGAPLTSKDVAYSIERMKTDKRSLQGGDIQELEVETPDDVTVVFTTKQPLS
                     LLLDRLDTRYIISKAAAEKYGDQADNYAIGTGPYKFVSWQRGGNLVLARNDDYWGAKG
                     EIKEVILKGVKEEAARVAGLLSGQADVVSNLPIEEIDRVAKHPRTRVEKVPGFRMYFV
                     AMNVTHKPFDNRFVRQAINHAIDPAALMKHIFSGNGEVLNGPLAANMTGFDPAMKRFA
                     YDPKKSRELLTKAGYPNGFETKLHFSPDRHLKGREVCEVIGNQLGKVGIKVELIGQEY
                     AVYWGKDGVNGGKLPFYYAGRTASDADTFYDQYFHTGITKRIGYSNPEVDKLIEEQQR
                     TADQKKRVTILQQAGRLLMDDAPFVPLYTLSEIYGVARNVIWKGTPDNRILAVEMKIR
                     G"
     gene            complement(3948..5441)
                     /locus_tag="VMZ02_06605"
     CDS             complement(3948..5441)
                     /locus_tag="VMZ02_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HUR71637.1"
                     /translation="MIRFSPLPNDAHIIPWREWSDEAFALARTEDKPVALFLGAFWCR
                     FCQRMDEQAFSDTEVIALLNAYFIPLRVDYMQRPDVDARYNLNGWPTIAFMTPNGQLL
                     AAGNYMPADQFKEWLIDVYMTYEQRKEELRGHEQIADQSQAQARQLVDAQLKANLAHI
                     TDVVMALADRTHGGYDRGQKFIHPQVNDFLLARYEATDDRQYLDQVCLTLERMRAGEL
                     YDHQGGAYFRTSSNPDWSHPHREKLLLEEAGLLSNCLTVFRITQRDEYRRMAEEIIQY
                     LDAKLYDPGNGAFYGCEDWLRHEPPTTDGDEFFTIIDRCIYTDANALASAAYLDAALL
                     LDQTRHHDRGLAVLEFLWQHCRSDSGAMVHYFDSAPQVPAWLQDQAQMGMALLRAYEV
                     TGDIDFLARARALAEFILTELKNPFGGFYDICAQDCASLTPRLTLIEQNGAAASFFLR
                     LAHDSNDEQYRAAAEHALSAFVGNFDAFGVHVAPFGRALDQLRQPSK"
BASE COUNT         1259 a         1608 c         1536 g         1197 t
ORIGIN      
        1 cggccggaat ccaggtttct ttgaccttac aacgctgtcg aggatggagc cggcctgcgc
       61 cggcatgaca aagattcacc tcgcttcaag gggagggatt tcaaccatcc ccaaaacggg
      121 gtagtcgttc agcttgagtc gggcggacgt tttcaatctg cgaaagattt ctcgcttccc
      181 tcgaaatgac agcagaatag tcgccttgtc atctcgaacg aagtgagaga tctatatcta
      241 actgaaccac taccaaaagg gacattaccc agggatttga cgggagatgc tcgcggagtg
      301 ctgattccaa gcgctagaga aacacgctga tattcttcgc atccagcacc gcttgatcga
      361 ggatgatcgt gcgtcgggcg atcttgaagg tcgtgccggc gcgccgcagg atatcctggc
      421 gtgtgccgac gaaaatatct ttgtcgcttt cctggcgcgt gcggtagacg agaaagttgc
      481 aatgggcctc gacttcggcg ctgtcatttt tttttatccg gacgttggta atcaggtgac
      541 gcgtgcgcga cggcggcact tcggcccagg cgatcttcga ataggcgcgc tcgacgcgaa
      601 tttttaaact tttccaatca tcgtcgaagt aataggcttg gccgggcggc gacacttcgt
      661 cggtgatatt ttccggccgc tccaatgggt tgtaccgaag cggcatccag tagcgaatat
      721 cttcggtcaa tagctcgaac cactggcgca gcttgcgctc gtcgagcagt tcggcttcga
      781 ggtagtagaa gtcttcgacc tgttggcgca attcgttatc catggattgc gcccgaaact
      841 gcgctccaac ttttcgcttc gagcatcttc gcccagtgca tgtacaggct cagttgattg
      901 ttgtcgctga aacggctttt cactcgtccg ggcaggtcga tttctatcgg cgcctcattg
      961 ccgctcatct gatagttggc cgggaaacgc tggccgatca aacttctcgc cgcgccggtt
     1021 acttggatcc agttgtccat gtcgtcttgc tcgaagacgc ccgacggact gaaacgatac
     1081 tgcgagacga agcgcatcac atctttgatt ttcggcgagg cggccttatc gacgatcgcc
     1141 cagctccaga tttccatctt gtccggtccg cgcggctgcc agacgcgaat cgtccgggtc
     1201 agccagtgca ccgacaaatt ggggaatacc gttcctgctg acgggacaat ttgccgcgcc
     1261 cgcttggttc ccaagcgttt ttcgatttcg gcttcatgct ctttcatgta atcgactagt
     1321 tcgtcgtccg gttgggcaaa ccacggtccg ccatccggcg gcgccaacca ggaaaccaga
     1381 atatgaccgt tatcgcaggc aatctgacaa cccttgttcc attgccgtgt tttgctggtc
     1441 gtcgtgtcga tgcccaattc ccgcgccgat ccgtgcgtcg acgcgatgtg gtagccgtcg
     1501 ccgccgaagt tttccgcggc ggttttccag ttggcgttga ccacccagcg ctgcggcccg
     1561 ctgacttctg tgccgccttc gcggcgatcg agcaggatgt ccaaatacca ggccatgtcg
     1621 ccgagatatt cgcgcagggt tggcgcgtcc ttgtcgaaag tagcaaagat cagccctttg
     1681 taactatcca cctgcgccac cgagactaag ccccactgcg agcgatcgag tttgccaaag
     1741 ctgtcgacca tcggcaccaa agcgagttta ccgtcggtcg tgtaggacca gccatgatat
     1801 gagcattgaa atagcttggt attgccgcga tcggcgcggc agacgcgatt gccgcgatgg
     1861 cggcacatgt tcaagtgagc ctgcagctcg ccgtttaagt cgcgcgccaa aatcaccggg
     1921 tcttcgccca tatagcgcgt gacgaaatca ccccggttgg gaatctcaca ctcgtgacca
     1981 agataaagcc aacagcgcgc gaagatgcgc tcgcgttcca agtcatagat atcgcggtcg
     2041 gcaaagattc gccgatcgat gagaccgcct tccgggtcga ccaatgattt gatgtccatg
     2101 gttaatcctc gctcgccggc acacgcttct ggtacggcag ttaataagct gcgctctctc
     2161 ctgtcaagca atgttattgc gacctctgct aagtgaattc ggaaagatcc ctcacttggt
     2221 gatgacaacc gcggtttttg tcatttcgag cacagcgaga aatctattca ttcccacggc
     2281 aaaagaccgg ccgaataaac gcaaaaacat tttgccgcgc cgctgccctg atgctataag
     2341 cctgccaggg agaacagcca tgagcacaga atcgaaaatc acccggcggc gctttctaca
     2401 acgcagctcc agcgttggtc tcggcgcatt gatcggactg acggctaatc gcagcgcgct
     2461 ggcagcctcc aaggagcggg ccgtgatcta ccaaggcgtt agtctcgact cgctgcatcc
     2521 ctacggctat agcggcggcg gcatcaacgg catttggctg cacctgatcg agccgctgat
     2581 cgtcatggac tacacacggc aaaaatatgt cggcatgctc gccgactcat gggaattcca
     2641 ggggcgaaga tggatcttcc aactcaggcg caatattcgt tttcacaacg gcgcgccgct
     2701 gacctccaaa gacgtggcat attccatcga gcgcatgaaa accgacaagc gcagcctaca
     2761 aggcggtgat atccaagagc tggaagttga aactcccgac gacgtcaccg tagtcttcac
     2821 caccaaacag ccactctcgc tcctgctcga ccgcttggac acgcgctaca tcatcagcaa
     2881 agccgccgcc gaaaaatacg gcgatcaggc ggataactac gccatcggca ccggaccgta
     2941 taaatttgtc agctggcagc gcggcggcaa tttggtcctg gcgcgcaacg acgactactg
     3001 gggcgccaag ggggaaatca aagaagtcat attgaaaggc gttaaagaag aagcagcgcg
     3061 ggtcgccggt ttgctctcag gccaagccga cgtagtcagc aatctgccca tcgaggaaat
     3121 tgatcgagta gccaaacatc cgcgtacgcg agtggaaaag gttcccgggt ttcgcatgta
     3181 tttcgtcgcc atgaacgtta cccataagcc attcgacaat aggttcgtgc gtcaggcgat
     3241 caatcatgcc atcgaccccg cggcgctgat gaaacatatc ttttccggca acggtgaagt
     3301 gctcaacggc ccgctggcag cgaacatgac cggcttcgat ccggcaatga aacgctttgc
     3361 ctacgatccg aaaaaatccc gcgaactgct gaccaaagcc ggttatccca acggcttcga
     3421 aaccaagctg catttttcac ccgaccgcca tctcaagggc cgggaagtct gcgaggtgat
     3481 cggcaaccag ctgggaaagg tcggcatcaa ggtcgaactg attggccagg aatacgccgt
     3541 gtactggggc aaggacggag tcaacggcgg caagctgccg ttttattatg ccggccgcac
     3601 ggcttccgat gccgacactt tctacgatca atattttcac accggcatca ccaaacgcat
     3661 cggctacagc aaccccgaag tcgacaagct tatcgaagag cagcagcgca ccgccgatca
     3721 gaaaaagcgc gtcacgatct tgcagcaagc cgggcgcttg ctcatggacg acgctccctt
     3781 cgtgccgctc tacacactgt cggaaatcta cggcgtggcg cgcaacgtga tctggaaagg
     3841 cacgcccgac aaccgcatcc tggcggttga gatgaagatc aggggatgac gatatgtgaa
     3901 gttgcagtac cggacgatgg gctcttaggc tgtctcgaca ctaacattca cttacttggc
     3961 tgtcggagtt ggtccagcgc tcggccgaac ggcgcgacgt ggacgccgaa tgcgtcaaag
     4021 ttaccgacga atgcacttaa ggcatgctcg gcggccgctc gatactgctc atcgttgctg
     4081 tcgtgagcca agcgcagaaa gaatgacgct gcagcgccgt tttgttcgat cagcgtcaaa
     4141 cgaggcgtga gcgacgcgca atcctgcgcg cagatgtcgt agaaaccgcc gaagggattt
     4201 ttcaactcag ttagaataaa ctccgccagt gcgcgcgcgc gggccaagaa atcaatgtcg
     4261 ccagtcactt cgtaggcgcg caacaaagcc atccccatct gcgcctgatc ctgaagccag
     4321 gccggcacct gcggcgccga atcaaagtaa tgaaccattg cgccgctatc agaccggcag
     4381 tgttgccaga gaaattccaa aacggccaag ccccgatcgt gatgccgtgt ttggtccagc
     4441 aataaagccg catcgagata tgcggcgctg gcaagcgcat tggcgtcggt gtagatgcac
     4501 cgatcgatga tcgtgaaaaa ctcatccccg tcagtggttg gcggctcatg gcgcaaccaa
     4561 tcttcgcaac cgtaaaaagc cccattcccc gggtcataga gcttagcgtc caagtattga
     4621 atgatttcct ccgccatgcg ccgatattcg tctcgctgag taatgcgaaa aaccgtcagg
     4681 cagttgctta gcagcccggc ctcttccagg agtaatttct cccgatgcgg atggctccaa
     4741 tcggggttgg acgaagtgcg aaagtaggca ccgccttgat gatcgtagag ctccccggcg
     4801 cgcatgcgtt caagcgttaa gcacacctga tcgaggtact gccgatcgtc ggtcgcctca
     4861 tagcgcgcca gcagaaaatc attaacctgc ggatgaataa atttctggcc gcggtcgtaa
     4921 ccgccatggg tgcggtccgc cagcgccatc acgacgtcgg tgatgtgcgc taaattcgct
     4981 ttcaattgtg catcgacaag ttggcgcgcc tgcgcttgtg actggtcggc gatttgctcg
     5041 tggccgcgca actcttcttt gcgctgctcg taggtcatgt acacgtcgat gagccactct
     5101 ttgaactgat ccgccggcat atagttgcca gcggcgagca actgaccgtt gggtgtcatg
     5161 aacgcgatgg tcggccaacc gttcagattg taacgcgcgt cgacgtcggg acgctgcata
     5221 taatcgacgc gtaacggaat gaaataggca ttgagcagcg ctatgacttc ggtatcggag
     5281 aaggcctgct catccatgcg ctggcagaac cggcaccaaa acgccccgag aaaaagcgcc
     5341 accggtttat cttccgtccg cgccaatgca aacgcctcgt cgctccactc ccgccacggg
     5401 atgatgtggg catcgttcgg aagcggcgag aaccgaatca tggcgcgatt gtgaaccgaa
     5461 tttacagcgg ccacaagttg tgatcgaaga cgtacaattg ttcttatttc ctccgcgtct
     5521 cggcagactg tatcgcaatt gtcatttcga ggcgaagccg agaaatctgt tcgtcgtatg
     5581 tggctgaaat tgcaagatcc
//
LOCUS       DATLCH010000224         6919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4303578, whole genome shotgun sequence.
ACCESSION   DATLCH010000224 DATLCH010000000
VERSION     DATLCH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4303578"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..2378
                     /locus_tag="VMZ02_06610"
     CDS             378..2378
                     /locus_tag="VMZ02_06610"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HUR71638.1"
                     /translation="MTTTYILKELSEPIRDSSGLDYRKELNEAQYEAATAIEGPLLII
                     AGAGTGKTRTLVYRVAHLIDQGIDPRSILLMTFTRRSAEEMIRRASLLMDGRCSQIAG
                     GTFHSFANLVLRVHGRHVNLASSFTIMDRPDSEDIIQLIRCDMGLNTKEKRFPRKQTV
                     AEILSMSLNKKIPLGDLLELEYPHLVEVNEEIGEIQRRYVDYKRSKSLIDYDDLLTKL
                     RDLLADHPDVRARLSNTYRFIMVDEYQDTNHLQAEIIRLLAATHDNVAVVGDDAQSIY
                     SFRGANFRNIMDFPKHFPGARIIKLEENYRSTQPILNLTNEIISRAKEGYEKRLFTRK
                     SVGTRPKLVQAGSEQMQSQFVCQKVLELREQGVPLWDIAVLFRSSFHSFDLEIELARH
                     NIPFVKRGGFQFMEAAHVKDLLAHLRIIANPQDAVSWNRVLMLLEGIGPGASQKIIKW
                     LLDGGSAVDRLRSYNAKGKIAQGLRTLAQVLEEAGQVELPAEQAQYLMQYYLPILKQN
                     YPDDHPKRLRDMEHFQGMTERYRSLERLLSDMALEPPNDSIGGVLAVDPDEGPLILST
                     IHSAKGLEFHSVFIIWALEGRFPSLYNSNSQEDLEEERRLLYVAATRAKQNLFISYPT
                     RVFDKTLRMVLSQPSQFIDGMSSRMLEPVALVPEGSYWNWES"
     gene            2360..3895
                     /locus_tag="VMZ02_06615"
     CDS             2360..3895
                     /locus_tag="VMZ02_06615"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HUR71639.1"
                     /translation="MELGELMVHFMHYVVVFVSGAVLMSLEIAGSRILAPNFGSSIFV
                     WGSLISVVMTALSIGYYWGGWLSAREPSYGKLAILLVIPGIVIFFLPFLYPAINDWIA
                     SIDLGNRLSPLFACGVLFLVPGVFLGTVSPYVIRLAATKLSTVGSTAGTLYAVSTAGS
                     IFGTLITAFYLIPVLGVSNIIHSLGVTLVVLSLIVVPLLRFQRAAVSRAVTASWLFLG
                     SFGYVSVASAPVAWAKTLLQKDTFYHRIRVEEDEEARYMYFDRTLQSAMSLKDPTALR
                     LVYSRYTSIAMTFRPDAKRMLLIGLGGGSIPKKFNKEFPNLVIDAIDLDPEVIRIAKD
                     HFNVGENKNLQLHAQDGRLFLARTQHQYDIIMLDAYFTDSMPFHLATKQFFELAQKKL
                     APNGLVVANLISAVTGPSGKIARSFVRTQRQVFPQTYIFASRRPDNVSLDTIQNVIVV
                     ATRDKQRVDIKDIVKRAMALDKGLFPDPVQDLAVAYFDRPLPEDVPILTDDFAPTDNL
                     LNP"
     gene            3924..4511
                     /locus_tag="VMZ02_06620"
     CDS             3924..4511
                     /locus_tag="VMZ02_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HUR71640.1"
                     /translation="MPPSAPERLVRVKALVDQPFRTRNPSWDREARGLIEAASDYYEE
                     EFGIRLITQAVSVWPEDERVPSTPTLLARLQKQFPFASADGSYDIVIAFTAENVSRIL
                     TAGRPRVDRIGNCSQGLARYIVLPTRKIFRYSGQSADLDPDVITLVHEIGHIFGAEHV
                     EDINSIMNENFDYRSQFDAKNRTIIKANRACAFAK"
     gene            4608..5366
                     /locus_tag="VMZ02_06625"
     CDS             4608..5366
                     /locus_tag="VMZ02_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HUR71641.1"
                     /translation="MLSNIRIVMVRPRGSGNIGSVARAMKNFGGADLAIVGTARTQSF
                     WAKAMAVHGRDVLSEAKCYDTIRAAIADCTLVVGTTCRAGLYRAHSQSPREIAPSIAA
                     AAKQGKIALIFGPEDHGLSNKDLEHCQLLVDIPSHPDYRSLNVAQAAVVCLYELYVAT
                     LTTPADGAIQRAEAESIERLFDIMRHALLKIGFLDSENPEHMLLAFRRIFGRAGLEDK
                     DVRILTGMFRQIEWYAKEGWKIVEQKEKQGIKIK"
     gene            5487..6125
                     /locus_tag="VMZ02_06630"
     CDS             5487..6125
                     /locus_tag="VMZ02_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HUR71642.1"
                     /translation="MNISDFNLKKTALLFFDILNGYVPAPAPGQPRVLKPWIENAVKL
                     SKAGRAAGLPVFFAKGNHRPDNATTALLLTDTNNSLTPWPNGEVTKTKMHVIAGDKSS
                     DVLADLGPLPDDYYIVKYRWSAFFQTYLDLALRSRGIDTLIVSGGSTDVGVTSTLYSA
                     RDLDYNTIVISDACGTSHDQRAHDMLMELVFPRMSRVRTTDQVLDMVAKARN"
     gene            6457..>6919
                     /locus_tag="VMZ02_06635"
     CDS             6457..>6919
                     /locus_tag="VMZ02_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71643.1"
                     /translation="MTSNGTAPVGISKNIKQIGRIDLPGGGSVVVENGYAYVGHMDPP
                     HGTSIIDVRDAKNPKIVAHLEIAEGLHSHKVRVSGDIMLVNYERYKAKEQLQSGLKVF
                     DISDKSKPREIAMYKTPGKGVHRFTYDGRYAYISPTVDGYQGNIAMMLDMQN"
BASE COUNT         1604 a         2024 c         1791 g         1500 t
ORIGIN      
        1 atagtttata gaatgatcaa cttagccatt cacaatctct gctgcgcaag gttcatacca
       61 gctgcgaata accaggctaa actgccgaga aaaaagcact aattttttta tcggcgtccc
      121 atcacaaatg acgacgcgag ggcgatgtat gagataccct acttggccgt ctgcgctgca
      181 ggccaattta actctgcgac aatggcaaat taaactgcgt cgagggatat ttttcgaatt
      241 gctttcccgt tcgcggcacc gtgccgtcga gcacccaagc gcgtaaaaaa tcgacacaga
      301 gtttactcgt gcaatttgct cggaacgctt gccggtgtga aagaaaatag gtactttacc
      361 gtgcaaccgg agcttacttg acgaccacct atattcttaa agagttaagc gaaccgattc
      421 gcgacagctc agggctcgac taccgcaagg agttaaacga ggcgcagtat gaggcggcga
      481 ccgctatcga agggccgctg ttgatcatcg ccggcgcggg taccggtaag acccgcacgc
      541 tggtctatcg agtcgctcat ctcatcgatc aaggcatcga tccaaggtcg atcctgttga
      601 tgaccttcac ccggcgttct gcggaggaaa tgatccgccg ggcaagcttg ttgatggacg
      661 ggcggtgcag ccagatcgcc gggggaacgt tccactcgtt tgctaatctg gtgctgcgcg
      721 ttcacggtcg acatgtcaac ctcgcctcgt cgttcaccat catggaccgg cccgacagcg
      781 aggacatcat ccaactgatc cgctgcgata tggggcttaa caccaaagag aaacgctttc
      841 cgcgcaaaca gaccgtggca gagatcttga gcatgtcgct caacaagaaa attccgttgg
      901 gcgatctgct cgaactcgaa tatccccatc tagtcgaagt gaatgaggag attggtgaga
      961 tccagcggcg ctatgtcgat tacaaaagaa gcaaatcatt gatcgattac gacgatctgt
     1021 tgaccaaact gcgcgacctg ctggcggacc atcctgacgt ccgcgcccgg ctctccaaca
     1081 cctatcgctt tatcatggtc gacgaatatc aggacaccaa tcacttgcag gcagagatca
     1141 ttcggctctt ggccgccaca cacgacaacg tcgcggtggt cggcgacgac gctcagagta
     1201 tttattcgtt tcgtggcgcc aattttcgca atatcatgga tttccccaaa cactttcccg
     1261 gcgcccggat cataaagctg gaagagaact atcgcagcac ccagccgatc ttgaacctga
     1321 ccaatgaaat catcagccgc gccaaggaag gctacgaaaa gcgcttattc acgcgcaaaa
     1381 gcgtcggcac ccgtcccaag ctcgtccaag cgggcagcga acagatgcaa tcgcagttcg
     1441 tttgtcaaaa agttttggag ctgcgcgagc aaggcgtacc gctgtgggac atcgccgttc
     1501 tgttccgctc gagctttcac tccttcgatc tggaaataga gttagcgcgt cataatattc
     1561 cattcgtcaa acgcggcggc tttcaattca tggaagcggc ccacgtcaaa gacctgctgg
     1621 cccatttgcg gatcatcgcc aatccgcaag acgcggtatc ctggaaccgc gtcctaatgc
     1681 tgctcgaagg catcggtccc ggcgctagtc agaagatcat caaatggctg cttgacggcg
     1741 gcagcgccgt cgaccggctg cgcagctaca atgccaaggg caaaatcgcc caagggctga
     1801 gaacgctcgc gcaagtcctg gaggaagccg gccaggtcga actgccggcc gaacaggcgc
     1861 agtatttaat gcagtactat ctgccgatcc tgaaacaaaa ctatcccgac gatcatccca
     1921 agcgtctaag ggacatggaa cacttccaag gcatgaccga acggtatcgc agcctggaac
     1981 gattattgag cgatatggcg ttggagccgc ccaacgatag cattggcggc gtgctggcag
     2041 tggatcccga cgaaggaccg ctgattctct cgacgatcca ctcggccaaa ggattggagt
     2101 tccattcggt attcatcatc tgggctctcg aaggacgttt tccatcgctc tacaactcca
     2161 actctcagga agatctggaa gaagagcgcc gcctgctcta tgtcgccgca acccgcgcga
     2221 aacagaattt gtttatttcc taccccactc gagtgttcga caaaaccctg cgcatggtgt
     2281 tgtcgcaacc gtcgcagttc atcgacggca tgtcatcgcg aatgctcgag ccggtcgccc
     2341 tcgtgcccga gggatcttat tggaactggg agagttgatg gtgcacttca tgcattacgt
     2401 ggtggtcttc gtcagcggcg cggtcctgat gtcactggag atcgctggca gccggattct
     2461 agcgccgaat ttcggcagtt cgatctttgt ctggggcagc ttgatatcgg tggtgatgac
     2521 ggcgctgagc atcggctatt actggggcgg ctggctgtcg gcgcgcgagc cctcctacgg
     2581 gaaactcgcc attctcctgg tgatccccgg catcgtaatt ttcttcctgc ctttcctcta
     2641 tccagcgatc aatgactgga tcgccagtat cgatctcggc aatcgtttga gcccgctgtt
     2701 cgcttgcggc gtgctcttcc ttgtgccggg agtctttctc ggcaccgtct caccgtatgt
     2761 cattcggttg gcagcgacta aattgagcac cgtcggcagc accgccggca cgctctacgc
     2821 ggtctccaca gcgggcagca ttttcggcac gctcattacc gctttttatt taattccggt
     2881 attgggcgtc agcaatatta ttcattcttt gggggtaaca ctggtggttc tatcgttaat
     2941 tgtagtacct ttgctgcgat ttcaacgtgc cgccgtctcg cgcgctgtca cggcgagctg
     3001 gctattcctg gggtcgtttg gttacgtcag cgtggcatcg gcgccggttg catgggcgaa
     3061 aaccttgttg caaaaagaca ccttctatca tcgcatacgc gtcgaagagg acgaagaagc
     3121 gcgctacatg tacttcgatc gcacgctgca gagcgccatg agcctgaaag atccgacggc
     3181 gcttcggctg gtctattcgc gctacacgtc cattgccatg acgtttcgtc ccgatgccaa
     3241 acgcatgctg ctgatcggtc ttggcggcgg ttcgattcct aaaaagttca acaaggagtt
     3301 tcccaatctc gtcatcgatg ccattgatct cgacccggag gtaatccgga ttgccaaaga
     3361 tcatttcaac gtcggcgaaa acaagaatct tcagctgcac gctcaagacg gccggttatt
     3421 cttggcgcgc acccagcacc aatacgacat cattatgctg gatgcctact tcactgattc
     3481 gatgccgttt catctggcca ctaaacagtt cttcgagcta gcacagaaaa aactcgcgcc
     3541 taacggccta gtcgtcgcta accttatcag cgcggttacc ggcccgtcgg gcaagatcgc
     3601 gcgttcgttc gtccgcaccc aacgacaagt ctttccgcag acttatattt ttgcatcacg
     3661 ccggcccgac aacgtcagcc tggacacgat ccaaaacgtc atcgtcgtcg ccacccgaga
     3721 caagcagcgc gtcgacatca aggacatcgt caaacgcgcc atggcgcttg ataaaggtct
     3781 atttcccgat cccgtccaag acctcgccgt cgcttatttc gatcgtcccc tgcccgaaga
     3841 tgtaccgatt ctaaccgatg attttgcgcc gacggacaat ctgctcaatc cttagcgcgc
     3901 tcgtcatcac cggctgctcg ctcttgccac cgtcagcacc ggagcgcctc gtccgcgtca
     3961 aggcgctggt ggaccagccg ttcagaacca gaaatcccag ttgggaccgt gaggcgcgcg
     4021 gtctgataga ggctgcgtcg gactactatg aagaggagtt cggcatccgg ctgatcacgc
     4081 aagcggtctc tgtctggccg gaggacgaaa gggtgccctc tacgcccacc ctgctggcgc
     4141 gcttgcagaa acaatttcca tttgcgtcgg ccgatggaag ctatgacatc gtcattgctt
     4201 tcaccgccga gaatgtcagc cgcatcctca ccgccggccg gccgcgtgtc gatcgcatcg
     4261 gcaactgcag ccaaggcctg gcgcgttata ttgtattgcc gacccgaaaa atttttcgct
     4321 acagcggaca gtccgcggat ctcgaccccg acgtcatcac gttggtccac gagatcggcc
     4381 atatcttcgg cgccgaacat gtcgaggaca tcaactcgat catgaacgaa aacttcgact
     4441 accgcagtca attcgacgcc aagaaccgca cgattatcaa agctaaccgc gcctgcgcgt
     4501 ttgccaaatg aacaaatagg caataggcat taggcaacag gcaatagtcg aatccggaac
     4561 tcggattttt ttcttgctgt tgcctagtgc ctgatgccta ttgccttatg ttgagcaaca
     4621 tccgcatagt catggttcgc ccgcgcggct cgggcaatat cggctcggtg gcgcgcgcaa
     4681 tgaaaaactt cggcggcgct gacttggcca tcgtcggcac agcgcgcacc cagagctttt
     4741 gggccaaggc aatggcagtg cacggccgcg acgtgctcag cgaagccaaa tgctacgata
     4801 ccattcgcgc cgccatcgcc gactgcacgc tggtcgttgg caccacctgc cgcgccggcc
     4861 tctatcgcgc ccatagccaa tcgccacgag aaatcgctcc gagtatcgcc gctgccgcta
     4921 aacaggggaa aattgcactc atcttcggcc ccgaagacca tggcctcagc aacaaagatc
     4981 tcgaacactg ccagctctta gtggatatcc cgtcccatcc tgactatcga tcgctcaatg
     5041 tcgcacaagc tgcggtggtg tgtttgtacg aactctacgt tgccacattg acgacccccg
     5101 cggatggcgc gatccaacgc gccgaggccg agagcataga gcggctattc gatatcatgc
     5161 gccatgcgct gctcaaaatc ggctttctcg actcggaaaa tcccgaacat atgctgctcg
     5221 ccttccggcg catctttggc cgtgccggcc tggaagacaa agacgtgaga atattgaccg
     5281 gcatgtttcg ccagatcgaa tggtatgcca aggaaggctg gaaaatcgtc gaacaaaaag
     5341 aaaagcaggg gatcaagatc aagtagatct attcagggcg atggccgcaa agaacgcaaa
     5401 gttcgcaaag ccggagagtt ttacagctgg tgaaattccc gtgatctata cgacggctga
     5461 aattgacagt tccaacagga ggacaaatga atatcagtga cttcaatttg aagaaaacag
     5521 ctttactatt tttcgacatt ctcaacggct atgtgcccgc gcctgccccc ggtcagccgc
     5581 gggtgttaaa accttggata gagaatgccg tgaagctcag caaagccggc cgcgccgccg
     5641 gattgccggt attctttgcc aagggcaatc accggcccga caacgccacc accgcgctgc
     5701 tgctcaccga caccaacaac tcgctcacac cctggcccaa tggtgaagtc acgaaaacca
     5761 aaatgcatgt catcgcagga gacaaaagct cagacgtgct cgccgacttg gggccattgc
     5821 cggacgacta ctacatcgtc aagtatcgct ggagcgcttt cttccagacc tatctcgatc
     5881 tcgcgttgcg cagccgcggc atcgacacgc tcatcgtctc gggcggttcc accgatgtcg
     5941 gcgtcacctc gactttgtat tccgcccgcg acctggacta taacaccatc gttatcagcg
     6001 acgcctgcgg cacgtcgcac gatcaacgcg cgcatgacat gctcatggag ctggtcttcc
     6061 cgcgcatgtc acgcgtgcgc acaacggatc aagtgctcga catggtggcg aaggcacgca
     6121 attagaatga caaactgttc accacgacgt gcgccgtcaa cagaggatga aatcgcaagg
     6181 aacctcactg cacatgaatt tttaccttac tcgtcctgcc ggcgcaggcc ggcatccagg
     6241 cgaggaggga tggataccgg ctttcgccgg tatgacggaa cctcggtcca tttcatcggc
     6301 gagatcttct ctgcacatgt attttcgaac gaggcacgta cgacgcgaag ttcggaatgt
     6361 tattttccca agaacttcgt gttcttcgcg ttttcgtggt gacatgcaat ttttatttgc
     6421 agcgtaccaa taacactttc attgaaggag tcatcgatga catcaaacgg caccgccccc
     6481 gtcggcatca gcaagaacat caaacagatc ggtcgtatag atctgcccgg cggcggctct
     6541 gtcgtcgtcg agaatggcta cgcctacgtc ggccacatgg acccgccgca cggcacgtcg
     6601 atcatcgacg ttagagatgc aaaaaatccc aaaatcgtag cccatcttga aatcgctgaa
     6661 ggactgcact cgcacaaggt gcgcgtcagc ggcgatatca tgctggtcaa ttacgagcgc
     6721 tacaaagcta aagaacaact gcaatccgga ctcaaggtct tcgacatctc ggataaatca
     6781 aagccgcgcg aaatcgccat gtacaaaact ccggggaaag gcgtgcatcg cttcacgtac
     6841 gacggccgtt acgcctacat ttcgccgacc gtcgacggct atcaaggcaa catcgccatg
     6901 atgctcgata tgcaaaatc
//
LOCUS       DATLCH010000225         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1348805, whole genome shotgun sequence.
ACCESSION   DATLCH010000225 DATLCH010000000
VERSION     DATLCH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348805"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VMZ02_06640"
     CDS             <1..665
                     /locus_tag="VMZ02_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71644.1"
                     /translation="TLILVGALLILARGRWENMVGAASAPPPSMGFFVTSAKSKTANL
                     GGLAGADKICQDLASAVGVGEKTWRAYLSADRDPANNNNPTNARDRIGTGPWFNANGL
                     MVGNDLADLHARRGNSILLVDERGQMVPGNWPNSPRPNEHDVLTGSTAEGMLMVGKTC
                     NSWTSESPDLRAQIGHIDGIGLGGNTAGASASWNSAHESRSCADTAPGGGAGRFYCFA
                     VK"
     gene            complement(1072..2100)
                     /locus_tag="VMZ02_06645"
     CDS             complement(1072..2100)
                     /locus_tag="VMZ02_06645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HUR71645.1"
                     /translation="MSNSHPIRFGIRIPNSGPLASPQSMLLIADEVEVLGFDSVWVHD
                     HLTWSDEIHRTHISSGSDDSNSNNSSPDFFEAMTSLAFIAGQVRKVRLGIACVVVPCR
                     NPLLSAKQIATLDVLCGGRLDIGVGIGSPSTIKSREYEVLGVNRKQRGKIADDHLRAM
                     KTIWSNHPASYDGQFVKFENAEICPKPLQKPNPPLWVGGWTEAAMKRTAEFGDGWLPA
                     WLLPEDIGKRFEEVKALAKTHGRDPEQIQLGIEVYGCIDEDGAKARRDGYGTLAIGQS
                     TFERMMSVEQLAAVSLIGSPEEIRKQVKAYSDAGVSHFEIKFIYPSLKRHSEMLQLFA
                     REVLPLFR"
     gene            2201..>2773
                     /locus_tag="VMZ02_06650"
     CDS             2201..>2773
                     /locus_tag="VMZ02_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71646.1"
                     /translation="MKLSRYSILFLCTAVATMTSIGMSTFSLFLPPIEKEFGLSRSMV
                     TLPYMVAMLGWAAGALTFGKLADDRGSRMVVLIGIALMALGFAGMGLSHNLWQLSLSY
                     GVCVGMAMGACSLVIMSLLVSKHFASNRGLAVAVIQTAPPMSPLLFAPIIYFLIRSYD
                     WRTAALLVSALLFLIAWPLAWIGARDPEGSQ"
BASE COUNT          557 a          730 c          830 g          656 t
ORIGIN      
        1 taactttgat tctggtaggt gcgctgttga tccttgccag aggccgttgg gaaaacatgg
       61 tcggtgccgc gagcgccccg ccgccgagca tggggttttt cgtaacgagt gccaaaagca
      121 agactgcgaa tctcggcggc ctcgctggcg cggacaaaat ctgccaagac ttagcgagtg
      181 ccgtcggggt gggcgaaaaa acctggcgag cctatttgag cgcggaccgc gatcctgcca
      241 acaataacaa tccaaccaat gcgcgtgacc ggatcggcac tggcccgtgg tttaacgcga
      301 acggcctaat ggtgggcaac gatctcgctg atcttcatgc gcgccgcggg aattccatct
      361 tgcttgtcga cgagcgcggc cagatggtcc ctggcaattg gccgaactcg cctagaccca
      421 acgaacacga tgtcctaact ggatcgacgg ccgaaggcat gctcatggtt ggaaaaacct
      481 gcaacagttg gacctcggaa tctcccgacc tgcgggcgca gataggccac atcgacggga
      541 tcgggctcgg cggcaatacc gcgggagcat cggcgtcgtg gaactcggct catgaaagta
      601 gaagctgcgc cgataccgct ccgggcggtg gcgccggaag attttactgt tttgcagtga
      661 agtagcctac attgacgccg aatgcctcaa gtcaggctgt taatccgatc cgagatcatc
      721 gcttcttcga tccggagctc gctatgcggt caacttttta tcgcggcgca cttgtactgg
      781 agtacagctg acgaatcgtt ggcactaatt cgacgtcaga aaaatgagca gattcaatgt
      841 cctctcgggg acagttgaaa tccttcgctc cttcgaaacc ttggggtcag gctgctagga
      901 aaagaagatt tgtcatgccg gcggaggccg gcatccaggt gtttttgccg agcaaagatg
      961 gattccggcc tgcgccggaa tgacgaatgg agagtcggct ttcagtcgac aaattcagac
     1021 tcctgggctt aaagccaagg atcattcagt agataaattt tgtttggcga tctaacgaaa
     1081 cagcggcaaa acttcgcgag caaatagctg caacatttcc gagtgccttt ttaaactcgg
     1141 gtagataaat ttgatttcga agtgcgaaac cccggcgtcg ctataggctt tgacctgctt
     1201 gcgaatttct tccggcgagc cgattagact gacggccgct agttgctcga cgctcatcat
     1261 cctttcaaac gtgctttgtc cgatggccaa cgtgccataa ccgtctcggc gcgccttggc
     1321 gccgtcttcg tcgatgcagc cgtagacttc gatgccgagc tggatttgct caggatctcg
     1381 gccgtgggtt tttgccagtg cttttacttc ctcgaatctt tttccgatat cttctggcag
     1441 gagccaggcc ggcaaccagc cgtcgccaaa ctccgcggtt cgtttcatgg ccgcttcggt
     1501 ccaaccgccg acccagagcg gcggattggg tttttgtaaa ggcttgggac agatctcggc
     1561 gttttcgaac ttgacgaatt ggccgtcgta gctagcgggg tgattggacc aaatggtttt
     1621 catggcgcgc agatggtcgt cggcgatttt gccgcgttgt tttcggttga cgccaaggac
     1681 ttcgtattcg cgcgatttaa ttgtcgaagg tgagccgatg ccgacgccga tgtcgagtcg
     1741 cccgccgcac agcacgtcga gggtggcgat ctgtttggcg gagaggagcg gattgcggca
     1801 ggggacgaca acgcaggcga tgccgagacg aactttgcga acttgaccgg cgataaacgc
     1861 cagtgatgtc atcgcttcaa agaagtccgg gcttgagttg ttgctgttgg agtcgtcaga
     1921 gcccgaggag atatgcgtgc gatgaatttc gtcggaccaa gtcaggtgat cgtgcaccca
     1981 taccgagtca aatcccaaca cctcgacttc gtcagcaatt agaagcatcg actgcggcga
     2041 tgccagcggg ccggagttgg gaatgcgaat gccgaatctg attgggtgag agttgctcat
     2101 ggatgggatt ccttctaacg gtaagtaata cgagtaaaac ccgcttgcaa tcggatgcta
     2161 aacattgcgg cgcaggtgcg gcactggcat agtcgtccgg atgaagctct cgcgctattc
     2221 gattcttttt ctctgcaccg ccgtagcgac catgacttcc atcggcatga gcacgttcag
     2281 tttgtttctg ccaccgatcg agaaagagtt cggcttgtcg cgctctatgg tgacgctgcc
     2341 ttacatggtc gcgatgctcg gatgggccgc gggcgcgctg acgttcggca aattggccga
     2401 cgaccgcggc tcacgaatgg tggttttgat cggcattgcg ctgatggcgc taggctttgc
     2461 cggcatggga ctgtcgcaca atttgtggca gctatcgctc tcgtatgggg tttgcgtcgg
     2521 catggccatg ggcgcgtgca gtttggtgat catgtcgctt ttggtctcga agcattttgc
     2581 cagcaatcgc ggtctcgccg tggcggtgat tcaaaccgcg ccgccgatga gcccgttgct
     2641 gtttgcgccg attatttatt tcctgattcg ctcctacgat tggcgcaccg cggcgttgct
     2701 ggtcagcgca ttgttattct tgatcgcttg gccgctggca tggatcggcg cgcgcgatcc
     2761 tgaaggctcg cag
//
LOCUS       DATLCH010000226         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2846522, whole genome shotgun sequence.
ACCESSION   DATLCH010000226 DATLCH010000000
VERSION     DATLCH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846522"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..2139
                     /locus_tag="VMZ02_06655"
     CDS             277..2139
                     /locus_tag="VMZ02_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HUR71647.1"
                     /translation="MKTAEIKPRSRKTNRSAKNTAIDRPANLGTPQMKWGSDVVAETT
                     RRLDLKYIALVPGASYRGFHDSIVNYLGNENPQMVICLHEEHAVSIADGYGKVTETPM
                     AVALHANVGLLHAAMPIFNAWCDRTPMIIFGATGPVDAHLRRPWIDWIHTAKDQGAVI
                     RDYTKWDDQPASAQAAVESVLRANQITRTAPRGPVYICLDAGLQESKLTEEIVIPDAS
                     RFAPGPTPAVSPASIQQTLKAVERAKFPLILAGRVSRDQGDWDRRVRLAEALGAAVLT
                     SSNDPSAFPTTHPLHFAAPCVRPNKAAAALIEKADVVISLDWLDLAGVLRLSLGKSQT
                     QEPAAKTIIHCSVDNYRTNGWSMDHQALPAVDIPVLAEPDQFVGQLLDALGAVKAQKL
                     TMRNEMKTLSHWNDTKFAKFSRNDEMTMWDMAMTMRDYAKGKAVTFARLPIGWPGEAY
                     EFDSPLSFMGNDGGGAVGTGPGHSIGTALALKDSGRLTIGVIGDGDYLMGVSALWTAS
                     HFNIPVMIVVADNRSYFNDELHQERVANMRNRPPQNRWIGQQIDNPRVDLVAMARAQG
                     FDSEAPVTTADELMKALKKGAAIVANGGRYFIDSVVQPGYADSGPDQRASLGKK"
BASE COUNT          535 a          615 c          662 g          512 t
ORIGIN      
        1 gcaagtatgc caaaccttaa tggtcctcgt tagtagttag tcctcgctaa aaagaagcgg
       61 ttacgacgca tcgtagccgg ccgcggatca gttacggaaa tttccgctag actcaggcgc
      121 tgcgttcaat atccgtagaa atcgtttcac accagaattt gcggctgcga ataattgtcc
      181 agcggcgaca gttgtgatag ctcctggttg gcaattaatt cgagatgagc ccggtccaac
      241 tgttcgcctt gccaacatta ttgaaggaga tcgctggtga aaactgcaga gatcaagccg
      301 agaagccgaa aaacaaatcg ttcagcgaaa aatacagcca tcgaccgccc ggcaaatctc
      361 ggcacgccgc aaatgaagtg gggcagcgac gtcgtcgcgg agaccacacg ccgtctcgat
      421 ctcaaataca ttgcgcttgt gcccggcgcg agctaccgcg gctttcatga cagcatcgtc
      481 aattacctcg gcaatgaaaa tccccagatg gtgatctgtc tgcatgaaga acatgcggtc
      541 tcaatcgccg atggttacgg caaggtcact gaaacaccga tggcagtcgc gttgcacgcc
      601 aacgtcggat tgctgcatgc agccatgccg atcttcaacg cctggtgcga tcgcacaccg
      661 atgattattt tcggcgccac cggtccagtg gatgctcatc tacgccggcc atggatcgac
      721 tggattcata cggccaagga tcagggcgcg gtaattcgcg actatacgaa gtgggacgat
      781 cagccggcct cggcgcaggc ggcagtcgag tcggtgttgc gcgcgaatca aatcacgcgt
      841 acagcgccgc gcgggccggt ttatatctgc ctcgatgccg gtctgcagga gtcgaagctt
      901 accgaagaga ttgtcattcc tgatgcgtcg cggtttgcgc cggggccgac accagcggtt
      961 tcaccggcgt cgattcagca gacgttaaaa gccgtagagc gggcgaagtt tccattgatc
     1021 ttggccggac gcgtgtcgcg tgatcaaggc gattgggatc gccgtgtgcg cttggccgag
     1081 gcgctcggcg ccgcggtatt gacaagcagc aatgatccct cggcatttcc gaccacccat
     1141 ccgctgcact ttgccgctcc ttgtgtgcgg cccaacaagg cggcggcggc attgatcgaa
     1201 aaagcagacg tggtcatcag tttagactgg ctcgatctgg cgggtgtgct gcgcttgagt
     1261 ttgggcaagt cgcagacgca agagccggca gcgaaaacta tcatacattg ctccgtcgac
     1321 aattatcgca ccaatggctg gagcatggat catcaagcgc tcccggcagt ggatattccc
     1381 gtacttgccg agccggatca gtttgtcggg caactgctcg acgcactcgg cgctgtaaaa
     1441 gctcagaagc tcacgatgcg caatgagatg aaaacgctca gtcattggaa tgataccaaa
     1501 tttgccaagt tctccagaaa tgatgagatg acgatgtggg acatggcgat gacgatgcgt
     1561 gattacgcca aaggaaaagc tgtgacgttt gctcgtctgc ccatcggctg gccaggggag
     1621 gcctatgagt tcgacagtcc gttatcattc atgggcaacg acggcggcgg cgcggttggc
     1681 accgggcccg gccacagcat cggcacggcg ctggcgctca aagattcagg ccggctgacc
     1741 atcggagtca tcggcgatgg tgactaccta atgggcgtga gcgctttgtg gaccgcctcc
     1801 cattttaata ttcccgtgat gatcgtcgtg gctgacaacc ggtcttattt caacgacgaa
     1861 ctacatcaag agcgcgtcgc taatatgcgc aatcgtccgc cgcagaatcg ttggataggt
     1921 cagcagatcg acaatcctcg cgtcgatttg gttgccatgg cgcgggcgca agggttcgac
     1981 agcgaagcgc cggtgactac ggccgacgaa ttaatgaaag cgctcaagaa gggcgccgcc
     2041 attgttgcca acggcgggcg gtactttatc gattccgtcg tgcaacctgg ttatgccgac
     2101 agcggtcctg atcagcgggc gagtttgggt aagaaataat ccgccagtta atcatcgcaa
     2161 gtccaatcat agcagttttg agttcgccga cgctccgtcc gcgcagcgtc tggttaaagt
     2221 aattcaattg ttgtgaataa agtttgttgc tagtacttga taaatcagcg gaatattact
     2281 tgacatatgt aatatttact tttaaagatt tgttatctct tcac
//
LOCUS       DATLCH010000227         7034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9997503, whole genome shotgun sequence.
ACCESSION   DATLCH010000227 DATLCH010000000
VERSION     DATLCH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9997503"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VMZ02_06660"
     CDS             complement(<1..507)
                     /locus_tag="VMZ02_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71648.1"
                     /translation="MPDARRLAIHGVEQHTMYRSKIVLASFLVVVAVVGAYLLIRSMN
                     TQSNRPLTAVDGYLRATYARDFAQVYEHLSSVDRHERPLQNFVNSQGPYSGFTLQVAR
                     QLAGFMEVWLIDRQDRNGRSVIKIGYRAPAPAELNNLLFNWDQERLNNLSNDQQKQLL
                     AELDRRSKS"
     gene            complement(518..1039)
                     /locus_tag="VMZ02_06665"
     CDS             complement(518..1039)
                     /locus_tag="VMZ02_06665"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HUR71649.1"
                     /translation="MLTRLILGYAIFMAMTANPSWAQHAHPAPHNIAPAIGSNAIPFD
                     LKTLEGKTAALESFHGKPLVINFFASWCDPCREEMPLINGLAAKASGSYQVLGIAVED
                     TRGAVTEYAKEAKLVFPIALDLNSTVKRAYRIFGPPATFFVDGEGIIRDIVLGPMTAD
                     RAKDAAKKVGIQR"
     gene            complement(1081..2406)
                     /locus_tag="VMZ02_06670"
     CDS             complement(1081..2406)
                     /locus_tag="VMZ02_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71650.1"
                     /translation="MKWAVDRWSSFCIFIICTGCGAELAQPTATTRSAQLKFTPEISL
                     GRSEANPSTPFLRYSPDGKLYAVWTEDHETPWPSGKPHPGHGPMSGDGAPSPMRNAVI
                     AASNDGGNTWTSAKRINSDIEAVQGEENGPKLAFDRNGKIFAVWSIPGAKGDKTRANI
                     RFAMEAGKGGFTPARTLNEVQGAGRFPIIETMPDNNLLIAWIDRRMDNPKPRQLYLMK
                     IDSRGRALTTNYQVGEGLCECCKLSVNFADAGQTVYIVDREVDAKKIRNHVLRKSIDG
                     GASFNVPVEISNDGWQVPSCPHSGPSIGRDSRGQLHVSWFTQGRSEAEAGIYYSMSND
                     GGKSFAPRTLIDANTAPETLYNNLVVGADDTIYFAWSNLDTNNRAQIYMRTLGADGKS
                     WGTIQQVSNAKGNANRATLALKGTQLNVAWTETDGEDSRVVLRSAEVSR"
     gene            2543..2836
                     /locus_tag="VMZ02_06675"
     CDS             2543..2836
                     /locus_tag="VMZ02_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71651.1"
                     /translation="MRKGRGVERLSYRDGLVTLNRKSTFDDDDGECVDDGGMGILRNR
                     QHEAVGWLWSWTCLMVAVFMLCPVGMLVTVFGSEGMFGYGESLEEMVHPMRCG"
     gene            complement(2976..3395)
                     /locus_tag="VMZ02_06680"
     CDS             complement(2976..3395)
                     /locus_tag="VMZ02_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71652.1"
                     /translation="MATRINVIIVLILALTLLPKIAQAQHQHGKEQTQAKTAPATAST
                     PPLQPAEGASIKILAPTKGQILKGDKIEIQYQLAKGKRGHHAHAYIDGELMGMFESTK
                     GTLNGVKPGKHVLELRVVASDHQTELDARDTVEFTTK"
     gene            complement(3521..4321)
                     /locus_tag="VMZ02_06685"
     CDS             complement(3521..4321)
                     /locus_tag="VMZ02_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HUR71653.1"
                     /translation="MSVSTFYIPFYLSCAIHIVVILLASIIIQDRPLRRQDYLQIGLV
                     DLPPTDTTAPKEKIAAPSESKKIQPTPPVVKYEKPQESPLLTSVPITNPDPLEPSIPP
                     AKQDATKTVDAKSLAPSARVEAGGSAAGGGNLFGTGDVGIVPGAGSAGGGGGTAAAGL
                     GRSSGAAGLPAQTVLRTNREAKPIQTVRAAYPMMALRAGLESDVSLRIEVDLEGRVTR
                     AEIVKSGGSGFDEEALKAVKQSRFEPAQRDGQNIPAEFTFVYRFRLQR"
     gene            complement(4509..5759)
                     /locus_tag="VMZ02_06690"
     CDS             complement(4509..5759)
                     /locus_tag="VMZ02_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71654.1"
                     /translation="MEHSDHNTPGAEPSGKTAIFSIVCGAHFLNHFQSSMLGVLYPLM
                     MRDLGIGFVAIGAITAVYGFVGNFLQAFFGFIVPYVRRGVVLGVGNILLGISVVATGF
                     ASSYAFIMVTRVLGGVGTSPQHPVGSTALASYFGKARGRALAFHSTAANLGSLVAPIF
                     AAVLVAHIGWRAIFWIVGIPSILLGFACLALRDTIRLNPAHIQKSKLTPVGLDAYKRC
                     LKNKNILLISLVLMVGGAGLGHGINETYIVPHFINDLKVDMAYAAFLFTLIQVGGLLG
                     PMAWGWISDRFHRTRTLQMSLLLSALSTVWLSLQDHVSIGLFANLILYGAVVTSRQTL
                     TQALLTDVAEEDILDAAFSLYYCIGFIMSPFWTIFAGWLMSTSGFQTTFGVVSCSYFL
                     GMTLLLFFREPVRKLIPLRPEPAH"
     gene            complement(5827..>7034)
                     /locus_tag="VMZ02_06695"
     CDS             complement(5827..>7034)
                     /locus_tag="VMZ02_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HUR71655.1"
                     /translation="TYNYTDANQSFTAQTWSSWYKLRLWSNFSLFLNNPVNGDGIEQN
                     DRRFLAGNNITYRRSYFLWGLATETLLGFQSRFDHIKLGLFNQEDRRRLSTTNDNGIQ
                     QTNLGWFAHQEIRLAHWVRGQLGMRLDNFWFKVTQDGPATEPISGAASKSMINPKMNW
                     IFTPFTDNDIARQTNLFVNMGGGFHSNDARIVVQDAAKALARYWSGELGVRTKLLNKV
                     DFSMSVWNSYLSSELVFVGDDGTFEPSGASRRQGIESEVRYDILPWLSLDSDISYTWA
                     KFVNGDKVPLAPRFLAFTGLTARHDSGLQARLQMRHIGPRYGIEDGSILTPTSTIFDL
                     FLKYTWKRYDFFMQFQNLANTKWRSAEHVFESRTPNELAAGLPGQNGSNYTPGDPFNV
                     RAGMTIHMW"
BASE COUNT         1550 a         1895 c         1957 g         1632 t
ORIGIN      
        1 gcttttgctg cgacggtcga gttccgccaa gagttgtttc tgttggtcgt ttgagagatt
       61 gttcaaccgt tcctggtccc aattgaacaa caaattattg agttcggccg gcgcgggtgc
      121 gcggtagcca atttttataa cgctacgccc attccggtct tgccgatcga tcagccagac
      181 ttccatgaag cccgcgagtt gccgcgccac ttggagtgta aagccgctgt aaggcccctg
      241 actgttcaca aagttctgca gcgggcgctc atggcggtcc acagacgaga ggtgctcgta
      301 cacctgagcg aagtcacgtg cgtaagtcgc acgcaggtag ccgtccacgg ccgtgagagg
      361 acgattcgat tgcgtattca tagatcgaat caacaaatag gcgcccacga ccgctacgac
      421 gactaggaag gaggcgagga cgattttgct acgatacata gtatgttgct cgacgccgtg
      481 aatcgctaac cggcgtgcgt ctggcatcaa gctgcattca acgctgaatg cctaccttct
      541 tcgccgcgtc tttggcgcga tccgcggtca tcggcccgag gacgatgtcg cgaatgatcc
      601 cctctccgtc gacaaaaaag gtcgccggtg ggccgaaaat tcggtacgcg cgctttacgg
      661 tgctgttcag gtcgagcgcg atgggaaaaa ccagcttggc ctcttttgca tactccgtga
      721 ccgcgccacg agtgtcttcc acggcgatgc cgagcacttg ataactgccg ctcgccttgg
      781 ccgccagtcc gtttatgagc ggcatttctt cgcggcaagg gtcacaccaa ctggcgaaga
      841 aatttataac tagtggcttg ccgtggaaac tttctaacgc cgccgttttc ccttccaagg
      901 ttttcaaatc aaatggaatg gcattgctgc cgatagcggg agcaatgttg tgcggtgccg
      961 gatgcgcatg ttgcgcccaa ctcgggtttg ccgtcatcgc catgaagatt gcgtacccta
     1021 agatcaatct agtcaacatg agtattcgcg gtttcgttaa ttttttagtg cgtcgcgagt
     1081 tcatcggctc acctccgcac ttctgagcac cacgcgcgag tcttcgccgt cggtttcggt
     1141 ccacgcgaca ttcaactgag ttcccttgag cgcaagcgtc gcgcggttgg cgttgccttt
     1201 ggcgttactg acttgctgga tcgtgcccca ggatttgccg tcggccccaa gcgtgcgcat
     1261 ataaatctgc gcccgattgt tggtatcgag attgctccag gcaaaataaa tcgtgtcgtc
     1321 cgcgccgacc acaagattgt tataaagagt ttccggcgcg gtgttggcat cgatcaacgt
     1381 tcgcggcgcg aaactcttcc cgccgtcgtt cgacatactg tagtagatcc cggcttctgc
     1441 ttcggagcgg ccctgcgtaa accaactgac gtgcagttgc ccacgactat cgcggccgat
     1501 gctcggaccc gaatgcgggc aggacggcac ttgccagccg tcgttgctga tttctaccgg
     1561 cacgttaaag ctggcgccac cgtcgatcga tttccgcagt acgtggttgc gaattttctt
     1621 tgcgtcgact tcgcggtcga ctatataaac tgtttggccg gcatcggcga aattgacgct
     1681 caatttacaa cactcgcaca ggccttcgcc gacttgataa ttcgtcgtta gcgctcggcc
     1741 tcgggaatca atcttcatca gatacaactg gcgcggttta ggattgtcca tgcggcggtc
     1801 aatccaggcg atcaatagat tgttatcagg catggtttcg ataattggga agcggccggc
     1861 gccttgaact tcgttcaagg tgcgcgccgg tgtgaagccg cctttgccgg cttccatggc
     1921 aaagcgaatg ttggcccggg tcttgtcgcc tttggcgcca ggaatcgacc aaacggcaaa
     1981 tattttgcca ttgcgatcaa aagccaattt cgggccgttt tcttcgcctt ggacagcttc
     2041 gatgtcgctg ttgattcgtt tcgccgaggt ccaggtgttg cccccatcat tcgatgcggc
     2101 gatgactgca ttccgcatcg gcgacggtgc accgtcgccg ctcatcggac catgaccggg
     2161 atgcggtttt ccgcttggcc agggcgtctc gtggtcttca gtccaaacgg catacaactt
     2221 accgtcgggg ctgtagcgca agaatggcgt cgaaggattc gcttccgacc tgccaagact
     2281 gatctcaggg gtaaacttca gctgtgccga tcgagttgtt gccgtcggct gcgcaagttc
     2341 cgcgccgcaa ccagtgcaga tgataaaaat acaaaatgaa gaccatctat cgacagccca
     2401 cttcatggga gactcctgcc taagtccctt aaggtttcca catataaggt tgttttcgcc
     2461 acatccctgt gacgaaaaca aacctcacta gttagcgaaa ttacttcgcc gtgatggaaa
     2521 cttaagctag cggcggtgcg cgttgagaaa agggcgaggg gttgaacgat tgagttaccg
     2581 cgatggcttg gtcaccctga accgcaaatc gacgttcgac gacgacgacg gcgaatgcgt
     2641 cgacgacgga ggcatgggca ttttgcgcaa tcgacagcac gaagcagtcg ggtggctgtg
     2701 gagttggacg tgtttgatgg tcgctgtctt catgctctgc cccgtgggca tgctcgtgac
     2761 tgtgtttggc agcgagggaa tgttcggcta cggggaatcg ctcgaagaaa tggtgcaccc
     2821 gatgaggtgc ggatgaagcc agaaaaaaac caaaaaccga gacgatgctc aaatataagc
     2881 cgcgtgcgct tgcgcaaact ggttttcggg ccttcatgtt tgtgcaaccg atggtaatcg
     2941 gatcgtgccg gcagtgcaac tcatgcgcag atcggctatt tcgtcgtgaa ctcgaccgtg
     3001 tcgcgagcgt ccaattcggt ctggtgatcg cttgcaacga cccgcaattc caagacatgc
     3061 tttcctggct tgaccccgtt caacgtacct ttagtgctct cgaacatgcc catcaattcg
     3121 ccgtcgatat aggcgtgggc gtggtgaccg cgtttaccct tagcgagttg gtactgaatc
     3181 tcgattttgt cgcctttcag tatctggcct ttggttggcg cgaggatttt aatgctggcg
     3241 ccttcggcgg gctgaagcgg gggagtcgat gccgtcgccg gcgcggtttt ggcttgtgtc
     3301 tgttctttgc catgttgatg ctgtgcctgc gcaatttttg gtaagagagt cagagctaat
     3361 atcaaaacaa taatgacatt gattcttgtc gccattctaa cattcctcta accgcgattt
     3421 caccatattt gacccattgg cgaaagccgc tcagtgactt tacccttaga cgttggagaa
     3481 gcccgcgccc cgatcgggcc taaaaaatgt ttttcgccgg tcaacgctgt aggcggaagc
     3541 gataaacgaa agtaaactca gcgggaatgt tttggccgtc gcgctgagcg ggttcaaagc
     3601 gcgattgttt cacagctttg agggcttctt cgtcgaaccc actgccgccg cttttgacga
     3661 tttcggccct ggtcaccctt ccttccaggt cgacctctat tctcaatgac acgtcgcttt
     3721 cgagtccggc gcgcagtgcc atcattggat aagccgcgcg tacggtttga atcggcttgg
     3781 cttcgcgatt ggttcgcaat acagtctgcg ctggcaaacc cgccgccccg gaactgcggc
     3841 ccaaccccgc agccgccgtg ccacctccgc cgccggcact tccggcgcct ggtactatgc
     3901 caacatcgcc agttccaaat aggtttccgc cgcccgctgc gctgccgccg gcttcgacgc
     3961 gagcgctggg agcaagcgat tttgcgtcaa ctgtttttgt tgcatcttgc ttcgccggag
     4021 gaatcgacgg ctctagcgga tccgggttcg ttatcggaac gctggtaagc agtgggctct
     4081 cctgcggttt ctcatattta accaccggcg gcgttggctg tatctttttg ctttcggacg
     4141 gggcggctat tttctctttc ggcgccgtcg tgtccgtggg cggtaaatcg accaggccga
     4201 tctgcagata gtcctgccgg cgcagcggac gatcttgaat gatgatcgag gcgagcagaa
     4261 tcaccacaat gtgaatagcg caggacaaat agaaggggat gtaaaacgtg ctaacgctca
     4321 cggcccggca ttccttcagc tactgtacac ttctcggttg gcggttctgt tccgaaaagc
     4381 caagattcaa tgacgaccgg tcaacgcacg gctcgagccg gcgagcagcg cgccatgacc
     4441 agtccatctt tgatgatgat atggagttaa acattagtca tttaagaacc gacgcagttg
     4501 gcagtctatc aatgcgccgg ctctggtctc agagggatga gtttccttac tggttcgcgg
     4561 aagaacagaa gcaacgtcat gccgaggaaa taggaacatg agacaacgcc gaaggtggtt
     4621 tgaaaacccg aggtgctcat cagccaaccg gcgaagatcg tccaaaacgg cgacatgatg
     4681 aaaccaatgc agtagtacag actgaacgcg gcatcgagga tgtcttcttc agcgacatcg
     4741 gtgagcagcg cttgggtcaa ggtctgccgc gaggtgacca ccgctccgta aagaatcaga
     4801 ttggcgaaca aaccgatcga tacatgatct tgcaggctca gccagaccgt cgacaacgcc
     4861 gatagcaaca gtgacatttg cagggttcgc gtgcgatgaa accgatcaga aatccagccc
     4921 catgccatgg ggccgagcag tccgccgact tgaatcaacg tgaacaagaa agcggcgtac
     4981 gccatatcca ctttcagatc gttaatgaaa tgcggcacga tgtaggtttc gttgataccg
     5041 tggcctaagc ccgcgccgcc gaccatcaat actaaagaga tcagcaaaat gtttttgttc
     5101 ttgaggcagc gcttgtacgc gtccagtcct acgggggtta attttgattt ctggatatgc
     5161 gctgggttca atcgaatcgt gtctcgcaag gcgaggcaag cgaagcccag aagaatgctg
     5221 ggaataccaa caatccaaaa aattgcccgc caaccgatat gcgctaccag caccgcagcg
     5281 aagattggcg cgaccaggct gccgagattg gctgcggtgg agtgaaacgc caaggcgcgc
     5341 ccgcgggcct tgccaaaata ggaggccaac gctgttgagc cgacgggatg ctgcggactc
     5401 gtgccaacgc cgccgagcac gcgggtcacc attatgaaag cgtaactcga agcaaagccg
     5461 gtggcgacga cggaaattcc cagtaaaata ttaccgaccc ctaagacgac gccgcggcgt
     5521 acgtaaggca cgatgaatcc aaaaaacgcc tggagaaaat taccgacaaa tccgtaaacc
     5581 gcggtgatcg cgccaatggc gacaaagccg atgccgagat cgcgcatcat cagcggatag
     5641 agcacgccca gcatggacga ctgaaaatga ttcaaaaaat gggcgccgca gacaatcgag
     5701 aatatggctg tcttgcctga aggttcggcg ccgggcgtat tatgatcgct gtgctccata
     5761 aagtttgttc tcatctcggg tcattgaatt agccgatcgc gctattttgc tttcatgcag
     5821 cgatgcttac cacatgtgaa tcgtcatccc cgctctaacg ttaaagggat cgccgggcgt
     5881 ataatttgag ccattctgcc cgggcagtcc ggcggcgagt tcgttaggtg tgcgcgactc
     5941 gaaaacatgc tcggccgagc gccatttggt attggccagg ttttgaaact gcatgaagaa
     6001 gtcatagcgc ttccaagtat attttaggaa caaatcgaaa atcgtcgagg tcggcgtcag
     6061 gatgctgccg tcttcgatgc cgtatcgagg gccgatgtgg cgcatctgca gtctcgcctg
     6121 gaggccggag tcgtggcgcg cggtgaggcc ggtgaaagcg agaaaacgcg gcgctagggg
     6181 aaccttgtcg ccgttaacaa acttcgccca agtgtaggaa atatccgaat ctaaggataa
     6241 ccagggcagg atatcgtaac gaacttcgct ttctatgccc tggcgtctgc tcgccccgct
     6301 gggctcgaaa gttccatcgt cgccgacaaa tacaagttcg ctggacaggt aggaattcca
     6361 aacagacatg ctgaaatcca ctttgttcaa tagctttgtc ctcactccca gctctcccga
     6421 ccaatagcgc gcgagcgcct tggctgcatc ctgcacaacg atgcgcgcgt cgttgctgtg
     6481 gaagccaccg cccatattga cgaacaaatt ggtttgacga gcgatgtcat tgtcggtgaa
     6541 cggcgtaaaa atccagttca ttttgggatt gatcatcgac ttcgaagcgg cgccggaaat
     6601 cggctcagtc gccggtccat cttgcgttac cttgaaccag aagttgtcga ggcgcattcc
     6661 caattgaccc cgcacccagt gagccagacg aatttcttgg tgcgcgaacc agccgagatt
     6721 ggtttgctga atgccgttat cattggtggt gctcaggcgg cgccgatctt cctggttaaa
     6781 taagcctaac tttatgtgat caaaccggct ttgaaagccg agtagtgttt cagtcgccaa
     6841 gccccaaaga aaatagctgc gccggtaggt gatgttattg ccggctagga aacgtctgtc
     6901 gttctgctcg atgccgtctc cgttgaccgg attattgagg aacaagctga aattcgacca
     6961 cagacgcagc ttgtaccatg aagaccaggt ttgggcggta aaactttgat tggcatccgt
     7021 atagttataa gtaa
//
LOCUS       DATLCH010000228         4961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7019950, whole genome shotgun sequence.
ACCESSION   DATLCH010000228 DATLCH010000000
VERSION     DATLCH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7019950"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VMZ02_06700"
     CDS             complement(<1..257)
                     /locus_tag="VMZ02_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71656.1"
                     /translation="MPYPSNIISAVDYVVEPSTVWTERLSESQFGKRIPHIERGTDGA
                     DRWIVDGRSFALAQTADVGALMSDRVTTTRWDDVPKAACDPL"
     gene            complement(379..1530)
                     /locus_tag="VMZ02_06705"
     CDS             complement(379..1530)
                     /locus_tag="VMZ02_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71657.1"
                     /translation="MEIRHGLISADSHVVTERNAFVDRMSKTVFGARIPQIKEVENQG
                     IPVERWFVNGKPLRTRGVANCPAVMGDPNRNLYPQRWEEVPAKAYVAADRLQALDSDG
                     VDAEVLFPNDPSSFHQYNDPDFEIACVQAYNDSLGELTRASERFISLAMIPLLCGMDT
                     IVAEVRRAQSKGHRGIVMFALPSLMVESLPHIGEPYWYPLWEICQELAMPVHFHASAG
                     MARKLTFPEWEGYTPYQQHAAFTVPTSSWPAQIVPQLIFSGIAYKFPRSKWVFAETGI
                     GSVSCVQSACDHEWERRQLWRHRLERRPSELIREQVYVNFWYEQAGIELRHVVGVDNI
                     MWESDYPHIASTYPDSRQFVERSLAGVAAEERKKMLYGNAQALYRLADH"
     gene            complement(1834..2514)
                     /locus_tag="VMZ02_06710"
     CDS             complement(1834..2514)
                     /locus_tag="VMZ02_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71658.1"
                     /translation="MSKWTDVERQLNVMVNQWYDTAEIKRMIAVPLTPARLAFRHQHM
                     SFFSNNRRDCWAAVACRSPLDVKRAIWEHEKDELIFDPRMGKAHVTEDDLGDKTESDM
                     IPGARAAFYAWLHCAQEKPWLEGLACSHILERRNNDQIVSGGTLTQRLVKRQEAELGI
                     KKQDQDINVQVHLVADVEHTDLLSEVFTRYVVDDLTAQQILRGAEESLAIERSFHGAI
                     ASAMAEME"
     gene            complement(2695..2940)
                     /locus_tag="VMZ02_06715"
     CDS             complement(2695..2940)
                     /locus_tag="VMZ02_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71659.1"
                     /translation="MPINNEEKSRYKISDSHRNQNFVGVMKRDRDTYSWTWKGHIDFA
                     DGHYFEFASQRSFETATEAEAYMRRYACDRIDSRMNV"
     gene            complement(3034..3693)
                     /locus_tag="VMZ02_06720"
     CDS             complement(3034..3693)
                     /locus_tag="VMZ02_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUR71660.1"
                     /translation="MPGYSVATSTYRIGGEDYQIRSLSDRRQFWDPDGQAERSGISSA
                     AWPIFGVVWPAGIALAQEMSHFAFAGLKILEVGCGIGLSSLVLQRHGADITASDYHPL
                     AEEFLRFNSDLNDLPPLKFQHAAWAGANPRLGRFDLIVGSDLLYERGHPALLSAFLTC
                     HTEAAAQVILADPGRNRCGQFSRKMAIEGYTQSEQRLDLTGDGSASPRGRMMSFVRGE
                     R"
     gene            complement(3755..4252)
                     /locus_tag="VMZ02_06725"
     CDS             complement(3755..4252)
                     /locus_tag="VMZ02_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR71661.1"
                     /translation="MIKLEPVGPANTVVFKDIRLRALQESPTAFSAKYADKAKLTESD
                     WLDRAAQWSSDKSAAYLAIDSGAPVGIAAGILDGNNPLHANLMSMWVAPTHRRLGIGR
                     ILVDAIISWARVHNVLHLHLMVTSNNDRAMQFYQRLGFTRTGRSQTYRNDASLLDYEM
                     RRPLL"
     gene            complement(4276..4740)
                     /locus_tag="VMZ02_06730"
     CDS             complement(4276..4740)
                     /locus_tag="VMZ02_06730"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HUR71662.1"
                     /translation="MPVDFVKKVFHFDSPTEKYHADACVVSCFDARFELATRKFMKKR
                     GVLMADPLKIAGGAKVFASPADESARIFALNQVQTSMRLHGTNLVLLMVHSDCSAYGG
                     LAAFDRDQEREAIHHESELRRAGDYLGRNIPSIEINCYFLRFTGVWAVEALD"
     gene            complement(4730..>4961)
                     /locus_tag="VMZ02_06735"
     CDS             complement(4730..>4961)
                     /locus_tag="VMZ02_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71663.1"
                     /translation="RRARMLDKASDAQMSIGDRSNSATFNRMQRKVQRAEAISQVKAE
                     MAGDNIDDKFAALEKQDEIEKLLNDIKARRAG"
BASE COUNT         1064 a         1360 c         1414 g         1123 t
ORIGIN      
        1 agcggatcac aggcggcctt ggggacgtca tcccaccggg tcgtcgtaac gcggtcgctc
       61 atcaaggcgc caacgtcagc cgtttgcgcc agcgcaaatg agcggccgtc aacgatccaa
      121 cggtcagcgc catcggtgcc gcgttcgatg tgaggaatgc gcttgccgaa ctgtgattcc
      181 gacaatcgct cggtccaaac cgtcgatggt tcgacgacat aatcgacagc gctgatgatg
      241 ttcgatgggt atggcataac gctatgcagg ggacgggctt atatttctca cctagttcgc
      301 tatgacaagc aaaaatatag ttgtcatttc gaccacaggg agaaatctat tcttcgacgt
      361 tccttggcaa tcttcatctc aatgatcagc aagtcgatac aacgcctggg cgttgccata
      421 caacattttc tttcgctcct ctgccgcgac cccggcaagg gagcgttcga cgaattgccg
      481 tgagtcggga taggtcgagg caatatgcgg gtagtcggac tcccacatga tgttgtcgac
      541 gccgacgaca tggcgcaact cgatgccggc ttgctcgtac caaaaattta catacacctg
      601 ttcgcgaatc agttcactcg gccgacgctc caagcgatga cgccataatt ggcgccgttc
      661 ccattcatga tcgcaggccg attggacaca actcaccgaa ccgattccgg tttcggcaaa
      721 gacccatttg gaccggggaa acttgtaggc gatgccggag aatattaact gcggcacgat
      781 ctgcgccggc cacgacgaag tcggtacagt aaacgccgcg tgctgttgat acggcgtgta
      841 accctcccac tcgggaaagg tcaacttgcg cgccatgccc gcggaggcat gaaagtggac
      901 cggcatcgcc aactcctggc aaatttccca cagtggatac cagtagggtt cgccgatatg
      961 cggcagcgac tcgaccatta aactcggcag ggcgaacata acgatgccgc gatggccttt
     1021 gctctgggcg cgtctgactt cggccacgat ggtgtccatg ccgcaaagca ggggaatcat
     1081 tgccaaggaa atgaagcgct cactggcacg agtgagttcg ccgagtgagt cgttataggc
     1141 ctgcacgcag gcgatctcga aatccgggtc gttgtactgg tgaaacgaac tgggatcatt
     1201 gggaaacagc acttcggcgt ctacgccgtc cgagtcgagg gcttggagcc ggtcggcggc
     1261 aacataagcc ttggccggca cttcttccca gcgctgcgga tatagattgc ggttgggatc
     1321 gcccatcacc gccgggcaat tggcgacgcc gcgcgtgcgc aggggtttgc cgttaacgaa
     1381 ccagcgctcg acagggatac cctggttttc aacttctttg atttgcggta tgcgcgcgcc
     1441 gaacacggtt ttcgacatac ggtcgacaaa ggcgttgcgc tcggtaacga cgtgggagtc
     1501 ggcgctaatc aggccgtggc ggatttccat tgcggtcctc ggtgagtaga ttaattttgt
     1561 gcattccgat ttccttcccc tcgatggaag agcctacgaa gggttgtctt gaattcatcg
     1621 tggaccgcaa ctcaattgga cactgtatcg caattgtcat ttcgagccgg aggcgagaaa
     1681 tttgcgtttg tatgtggacg agaatgcgag atctgtcgct gactttggag atgactatgc
     1741 gcacgattcc agaatgcggc acagtctctc aagggcgaag atgttcgctc ggccgctgct
     1801 ggtaagtgac aactatgaac cgccaagcgc ttgtcactcc atctccgcca tcgcgctggc
     1861 gatggcgccg tgaaagctgc gctcgatggc aagcgactcc tcggcgccgc gcaggatttg
     1921 ttgcgcagtc aggtcatcga ccacataacg tgtgaagact tcagacagta ggtcggtgtg
     1981 ctctacatcg gcgacaagat ggacttgaac gttgatgtct tggtcctgct tcttaatacc
     2041 tagttcggct tcctggcgct tgacgagccg ttgggtgagc gtaccgccgc tgacgatctg
     2101 atcgttattg cgccgttcca aaatgtgtga gcaggccagt ccttcgagcc agggtttctc
     2161 ttgggcgcag tggagccaag cgtaaaatgc cgcgcgcgcg cccgggatca tgtcggattc
     2221 agttttgtcg cctaaatcgt cctcggtgac atgcgccttg cccatgcgcg gatcgaagat
     2281 caattcgtcc ttttcgtgtt cccagatggc acgtttgacg tcgagcggag atctgcaggc
     2341 cacggcggcc caacaatcgc gccggttgtt gctgaaaaaa ctcatatgct ggtggcgaaa
     2401 ggcgagccgg gcaggagtga gcggcacggc gatcatgcgc ttgatctccg ctgtgtcgta
     2461 ccactggttt accatgacgt taagctggcg ttcgacgtca gtccatttgc tcatcttatt
     2521 attcccaaac gaaattcact tgtcgcgcca cggcgcgcgc taaccggaat tgttaaccgg
     2581 aatatatgtc ggtacggaaa gtactgtcaa tcaggaccgg gcgagagatt ccggatgctg
     2641 ccgaggaact ggcgagatta taaccggggt gttttgttgt gaagcggcgc cgtcctagac
     2701 gttcatacga ctatcaatgc ggtcgcacgc gtaacggcgc atgtaagctt cggcttcggt
     2761 ggcggtttcg aaactgcgct ggctggcaaa ctcaaaatag tgcccatcgg caaaatcgat
     2821 gtggcctttc cacgtccacg agtaggtatc gcgatcccgt ttcatcacac cgacaaagtt
     2881 ctggttacgg tgcgaatcgc taattttgta acgggatttc tcttcgttat tgataggcat
     2941 ggtcctcagt gtagtgcttg gcacgcgcga tagcaatcaa ctatttgcaa caattgaact
     3001 cgaaaagtaa caattaccgc aacagcgtgg catttatctt tcgccgcgaa caaaactcat
     3061 catccgcccg cgcggtgacg cactcccgtc gccagtcagg tccagccgct gttcgctctg
     3121 cgtgtagcct tcgatcgcca tttttctgct gaattggccg cagcggttgc ggccggggtc
     3181 ggcaaggatg acttgggcgg cagcttcggt atggcaggtg agaaatgccg acagtagggc
     3241 aggatgccca cgttcgtaga gaaggtcgct gccgacgatc agatcgaaac ggccaagccg
     3301 gggattggct ccggcccagg cggcgtgttg gaatttaagc ggcggcaagt cgtttaaatc
     3361 actgttgaag cgaagaaact cttccgccaa tggatgataa tcgctggcgg taatatcggc
     3421 tccgtggcgc tgcaatacca aactcgacaa tccgatgccg cagccgacct cgaggatctt
     3481 caacccggca aaggcaaaat gactcatctc ttgcgccaac gcgatgccgg ccggccatac
     3541 gacaccgaag atcggccagg ctgccgatga aatgccgctg cgctccgctt gaccatcggg
     3601 atcccagaac tgccggcggt cgctcaacga gcgaatctga tagtcctctc caccgatgcg
     3661 gtaggtcgag gtcgcgaccg agtatcccgg catgcgaata ttccttagcg tttttgtaac
     3721 gccgctagca gttgcacaga cgataactcg gaaatcagag aagaggccgg cgcatttcgt
     3781 agtcgagcag cgacgcatca ttgcgatagg tttggcttcg accggtgcgc gtaaagccaa
     3841 gacgctggta aaattgcata gcgcgatcgt tgttgctggt caccatcaaa tgtaagtgta
     3901 gcacattatg tacacgcgcc catgaaataa tcgcatcgac taagatgcgg ccaattccca
     3961 ggcggcggtg cgtcggcgcc acccacatgg acatcaggtt ggcgtgcaga ggattgttac
     4021 cgtcgaggat tcccgccgca atgccgaccg gagcaccgct gtcgatcgcc aaataggccg
     4081 cggacttgtc gctgctccac tgggccgcac ggtcaagcca gtcgctctcc gtaagtttgg
     4141 ccttgtcggc gtatttagca ctgaatgccg tcggcgactc ctgtaatgca cgcaggcgaa
     4201 tgtctttgaa gacaaccgtg ttggctggac cgacgggttc aagtttgatc atcttccgtt
     4261 gcgagcgcgc aattatcaat ctaacgcttc gacagcccaa acgcctgtaa acctgagaaa
     4321 atagcagtta atctcaatcg atgggatatt tcttcccaaa tagtcgccag cccggcgtaa
     4381 ctccgactcg tggtggatgg cttcgcgctc ttgatcgcga tcaaaggcgg ccaatccgcc
     4441 gtaggcactg caatcgctgt ggaccatcag tagcacaagg ttggttccgt ggaggcgcat
     4501 tgaggtttgt acttggttaa gcgcgaagat acgcgcggac tcatctgccg gagaggcaaa
     4561 gacttttgct cctccggcga ttttcagtgg gtcagccatc aggacgccgc gctttttcat
     4621 gaattttcgt gtcgctagct cgaagcgggc gtcgaagcac gaaaccacgc aggcgtcggc
     4681 gtgatatttt tcggtcggcg agtcgaagtg aaagaccttc ttcacgaaat caaccggcac
     4741 gccgagcttt tatatcgttg agcaacttct cgatttcgtc ctgtttttcc aatgcggcaa
     4801 acttgtcgtc gatgttgtcg ccggccatct cggctttcac ctggctgatc gcttcggcgc
     4861 gctggacttt tctctgcatc cgattgaaag tcgcgctatt ggagcgatcg ccgatcgaca
     4921 tctgcgcgtc gctcgccttg tccaacatac gcgcccgccg g
//
LOCUS       DATLCH010000229         4019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1368803, whole genome shotgun sequence.
ACCESSION   DATLCH010000229 DATLCH010000000
VERSION     DATLCH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368803"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VMZ02_06740"
     CDS             <1..373
                     /locus_tag="VMZ02_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71664.1"
                     /translation="RFIDGEHHTADIETNHYDLVLASNFCRFESPKGNQELFAKAFSA
                     LKPGGVFVVNDFVPNEERTEPTFALRFSVYTLTHTPEGECWTLSQYADWLKQAGFAAI
                     KTYGDIPKTLPGTTLILATKG"
     gene            complement(439..1347)
                     /locus_tag="VMZ02_06745"
     CDS             complement(439..1347)
                     /locus_tag="VMZ02_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71665.1"
                     /translation="MVAEIRYGVETIDHIAIPVRDLATNQSFYTETLGLTFKTTRRNP
                     DGTPRQTYVLAGANIIGLHLPGINAAPAASAAPRIGIGVTSEEFDQVQQNLLLAKHRF
                     KGPIQHSPEAVISRSIYFDDPDGNHLELCVRRVEPFNQCISHTVFETCNMTKALTFYS
                     EVLGTAVPIRFGDEMLIPTRNGQMIGLLEVAELSDRSKKHGRGCHMAMDVPQEDFASM
                     VALVERYGGRTQGDKRADEGLRPAGEQSIYLFDPDNNRLQITAHAANHKEELLDDAEK
                     WRRIIAARKKQGKGLTRWESGGKKLL"
     gene            complement(1451..2680)
                     /locus_tag="VMZ02_06750"
     CDS             complement(1451..2680)
                     /locus_tag="VMZ02_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HUR71666.1"
                     /translation="MINSGERGFGLGKFSGAERERRWSRVRELMARAHLDAIIGFPNQ
                     SHWDQFQADIRYLTHIGGHQTEVAMVFPLQGEPTAFVRGSNEIEWWRIAQDWVADIRP
                     SRREWSPPMIARMRELKMDRNRIGVSGLSGLLRAPEGTVVSGMLDRVRGAFPNAQFEN
                     ATELLQDARSIKGSEEIAWMERAAEILDEVVAAILGKARPGVMENDMVATIWQTIIAN
                     GGDYPSMTHWGAGQGVPWACRIAPHRPLQAGDMINTELEAKYGGYISQTVQAACLGQI
                     PTDLKRAFDASVNVFDELVNFMEPGVTYRAVVELYQKLVREAGFIPKGMLLHGRGIGE
                     DRPQITGEVAGDMYTSATYTQHLDLPLREGNVFVLKPGAMPGDAPDSIRCGDTVVIEK
                     TGARRLGKRRFTFPEIG"
     gene            2865..>4019
                     /locus_tag="VMZ02_06755"
     CDS             2865..>4019
                     /locus_tag="VMZ02_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HUR71667.1"
                     /translation="MQYLAICEDDLLFQFMRNVATEQGDVRYLVPNEKVAQRIKRQRG
                     KVAHGDLLKEETYKKIKLHHVDQAIVFVRDVDEQDRVCRLLRTLDKNVSIVSLISEKN
                     GKKPAKEPDDPLLRHLQLGDIFAEFCATQLLDTLNVKKVERIRSLFQEGQRIHILLQH
                     DPDPDAIGSALALRELLGRNRTTTPIVTFGSVTRPENLAMIELLEIQIDQITPDDLHK
                     DGARLALVDVQPPYFEQPIGRVDLVVDHHPKRTNFKARFADLRSGYGSTATIFTEYLR
                     AAGMEPSQRLATALLYGIKTDTLFLERGSNLADLSAFTYLYPIANKAMITRIERPALP
                     REDLEAMGRALTHLQIDGGVAVIHLGEVNREDVIPQMAEFCLQIQGVDWGV"
BASE COUNT          845 a         1127 c         1114 g          933 t
ORIGIN      
        1 tcgctttatc gacggtgaac accacactgc cgacatcgaa acgaatcatt acgatttggt
       61 tctcgccagc aacttctgcc gcttcgaatc gcccaaaggc aatcaggaac tgtttgccaa
      121 agcgttttct gcgttgaagc cgggcggagt cttcgttgta aatgattttg tgccaaacga
      181 agagcgcacc gaaccgactt tcgcgttgcg tttttccgta tacacgctga cgcacacgcc
      241 ggaaggcgag tgctggacct tgtcgcagta tgccgattgg ttgaagcagg cgggattcgc
      301 ggcgatcaaa acttacggcg acattcccaa gaccttgccg ggaacaacct taattctcgc
      361 aaccaagggt taattccgag ttgcacgaca aagcagagac ctgattcctg cgctggtatg
      421 aatccgtcgc tgaaagtttt agagcagttt cttaccgccg ctttcccagc gtgtgaggcc
      481 tttgccctgt tttttgcggg cggcgatgat gcggcgccat ttttctgcgt cgtcgagtag
      541 ctcttccttg tggttggctg cgtgggcggt gatttgcagt cgattattat cgggatcgaa
      601 aaggtagata ctctgctcac ctgctggtct tagaccttcg tcggcgcgct tatcgccttg
      661 ggtcctaccg ccatagcgtt caaccagcgc gaccatggaa gcgaaatctt cctgcggtac
      721 gtccattgcc atatgacagc cgcgaccgtg tttcttgctg cggtcggata attccgcgac
      781 ttccagaaga ccgatcattt gaccgttgcg cgttggtatc agcatctcat cgccaaaccg
      841 gatcggcaca gccgtgccga gcacttcaga gtaaaacgtg agtgcctttg tcatattaca
      901 ggtttcgaaa actgtgtgac tgatacattg gttgaaaggc tcgacccggc gcacgcacag
      961 ttctaaatga ttaccgtcgg gatcatcgaa atagatagag cggctaatca ccgcttcagg
     1021 cgaatgttgg attgggcctt tgaatcgatg ctttgccaaa agtagatttt gttgaacctg
     1081 gtcgaactcc tcgctcgtga cgccgattcc tatgcgaggc gcggcgctgg cggcaggagc
     1141 agcgttgatg cccggcaagt gcaggccgat tatatttgct ccggcaagaa cataggtttg
     1201 ccgcggagtg ccgtccgggt tacggcgggt ggttttgaaa gttagcccca aagtttcagt
     1261 ataaaaactt tggttggtcg ccagatcgcg caccgggatg gcgatgtgat cgatggtttc
     1321 gacgccgtaa cgaatttctg cgaccattcg actacctccg aggtttaggt caattttccg
     1381 ggaacgctaa ctcgattaac tctgcagcgt caacccttta gaaacttttt atcccggcaa
     1441 gggtgattct ttagccgatt tctggaaacg tgaatcgtct tttgccgagc cgccgcgcgc
     1501 cagttttctc gatcactacg gtgtcaccgc agcggatcga gtcgggcgcg tcgccgggca
     1561 tggcgccggg cttgagcacg aagacattcc cctcgcgcag cggcagatcc aagtgttgcg
     1621 tatacgtcgc gctggtatac atgtcgccgg cgacttcgcc ggtgatctgt ggtcggtctt
     1681 cgccgatacc gcggccatga agcagcatgc ctttgggaat gaaaccggcc tcgcgcacga
     1741 gcttctggta aagctcgaca acggcgcggt aagttacgcc gggttccata aaattcacca
     1801 gttcgtcaaa gacgtttaca ctggcgtcga aggcgcgctt caaatcggtg gggatctggc
     1861 ctaaacaagc ggcttgcacg gtctgggaaa tatagccgcc gtacttcgct tccagttccg
     1921 tgttgatcat gtcgccggct tgcagcggcc gatggggggc gatgcggcag gcccacggca
     1981 caccctgacc cgcaccccag tgggtcatcg acggataatc gccgccattg gcaataatcg
     2041 tctgccaaat cgtcgcgacc atatcatttt ccatgacgcc gggccgggct ttgccgagga
     2101 tcgcggcgac gacttcatcg aggatctccg cagcgcgttc catccaggca atttcttcgc
     2161 tccctttgat cgagcgggcg tcctgtaata attcggtggc gttctcgaac tgagcattgg
     2221 ggaacgcgcc acgcacacgg tcaagcatgc cgctgacgac ggtgccttcg ggtgcgcgca
     2281 agagaccgct taagccgctg acgccgattc tgttccggtc catcttcaat tctctcatgc
     2341 gcgcgatcat cggcgggctc cattcgcgcc gcgacgggcg aatatcggcg acccagtctt
     2401 gcgcaatgcg ccaccactcg atttcattgc tgccgcggac aaacgctgtc ggctcgcctt
     2461 gcagtggaaa caccatggcg acttcggttt gatgaccgcc gatgtgagtc aggtaacgaa
     2521 tgtccgcttg aaactgatcc caatggctct ggttgggaaa gccgatgata gcgtcgagat
     2581 gcgcgcgcgc cataagctcg cggacccgcg accagcgccg ctcgcgttcc gctccgctga
     2641 atttacctag gccaaaacct ctttcgccgc tgtttatcat gctttaggtg ctctctttct
     2701 ttcgattatt cgttatctta taggcgggtg gtctccgccg atcaatggtt tgcctagctt
     2761 tgatctgagc taggtgctag agtcccgccg tgccaacgaa ccgccaagcc gcttacttcg
     2821 ccgcgcgagt cggtcgcatc taaatcccgc tcacgggatg cgctatgcaa tatctggcta
     2881 tctgcgagga cgatctgttg tttcagttca tgcgcaatgt cgccaccgaa cagggcgacg
     2941 tgcgttatct ggtgccgaac gaaaaagtcg cccagcgcat caaacggcaa cgcggcaagg
     3001 tcgctcatgg cgatctcctc aaagaggaga cctacaagaa aattaaactg caccatgtcg
     3061 atcaagccat cgtttttgtt cgcgacgtcg acgaacaaga ccgggtatgc cgcctattgc
     3121 gcactctcga caaaaacgta tcgatcgttt cattgatttc ggaaaagaac ggcaagaagc
     3181 cggcgaaaga acctgatgat ccgttactgc gacacttgca gcttggcgat atttttgccg
     3241 agttttgcgc cactcaactg ctcgacacgc tcaacgttaa gaaggtcgaa cgcattcgct
     3301 cgctgttcca agaaggccaa aggatccaca tccttttgca gcatgatccg gatccggacg
     3361 ccatcggtag cgcattggca ttgcgcgagt tgctcggacg caaccgcacg accacaccga
     3421 tcgtcacctt cggttcggta acacggccgg aaaacttggc gatgatcgaa ctgttggaaa
     3481 ttcaaatcga tcaaatcact ccagacgatc tacacaaaga tggcgcgcgc ctcgccctgg
     3541 tcgacgttca accgccgtac tttgagcagc ctatcggacg ggtcgatttg gtcgtcgacc
     3601 atcatccgaa gcggaccaat ttcaaggcgc gcttcgccga cttgcgatcc ggttacgggt
     3661 cgactgcgac gatatttacc gagtatttgc gtgccgcggg catggagcca tcgcaacgtt
     3721 tggcgacggc gctgctctac ggcatcaaga ccgatacatt gtttctcgaa aggggcagca
     3781 acttggcgga cctgagcgcg tttacctacc tgtatccgat cgccaacaaa gcgatgatta
     3841 cacgtatcga acggccggcg ttgccgcgtg aagacctcga agcaatgggc cgggcgttga
     3901 cccacctgca aatcgatggc ggtgtggcgg tgattcactt gggcgaggtc aaccgcgaag
     3961 acgtgatacc gcaaatggcc gagttctgtc tgcagatcca aggcgtcgac tggggtgtg
//
LOCUS       DATLCH010000230         3280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5836183, whole genome shotgun sequence.
ACCESSION   DATLCH010000230 DATLCH010000000
VERSION     DATLCH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5836183"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..873
                     /locus_tag="VMZ02_06760"
     CDS             250..873
                     /locus_tag="VMZ02_06760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR71668.1"
                     /translation="MGVKEVPSDHDFFDPAYVKHWTESISRYRPERKRLFEVFAAEAA
                     RIKNGALSIFELGCGPGFLAEALLNNCPNARYTLVDFSPQMLDLSRSRLAKFKDRTVF
                     IQADFKKADWIVGIGAGFDVVVSLQAVHELRHATRIPKLYAQLHSLLAPGGTILICDH
                     VNSSSSHRAAHFMTVEEHLSTFKKAGFINAREICAAADLSLMAAEKP"
     gene            976..1371
                     /locus_tag="VMZ02_06765"
     CDS             976..1371
                     /locus_tag="VMZ02_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71669.1"
                     /translation="MENTKTDPIVIEQPGMRMTIEHRSGLGGNDEGLTFDVTTAESAD
                     AGKRILRFDCFYKNPHYHVGASGGEAAAYKMKDEGIDDPVRWTLEQLKTRFPAMVRQA
                     GYEEIARTIDQQAITDQLTRLEQKIIDKY"
     gene            complement(1755..2756)
                     /locus_tag="VMZ02_06770"
     CDS             complement(1755..2756)
                     /locus_tag="VMZ02_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71670.1"
                     /translation="MALKKLAAGLILIPLSVIDICQLTFALDRVTVGLSSTSSVNGAI
                     WVAEERGLFKKHGIDVNVTNVGGGAARAISAMVGGDIQFATAGGAAAISASLAGADVV
                     MVASILNKGVQKLIARPPIKNPKEIIGQKIGIVGFGSASHTVLQLLLQRWNIGIDKVQ
                     LVTWESSPSMLIALDKGTIDGGVFTEPTVFMAEEKGYNVLADLVDMDIYYLQNALAVT
                     RSHLRSHNDPATRFVKGYVEGIAYFKRNKKESLEVLKKKLRTEPAQEKYLEKSYDLLA
                     STYYNKVPYPSIPGVKTVLEFLGKDNAKAKGADPNSFVDSSIVKDLDQSGFINKLYD"
     gene            complement(2957..>3280)
                     /locus_tag="VMZ02_06775"
     CDS             complement(2957..>3280)
                     /locus_tag="VMZ02_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71671.1"
                     /translation="DVLTMNRHGRRWMRYAIGGVEAIPEESKDQYTQPTRIKWDKVIL
                     LGDVIAGNHPGRTSDRQITGLVLRGDGVQFTAVGHLIYHRCKELGLGTEIPTELFLQD
                     EKYIP"
BASE COUNT          765 a          898 c          884 g          733 t
ORIGIN      
        1 tgtaaaagtt gtcagccctg gcacaggtga gtcacgggat ctcgaccggc ggtttgagaa
       61 tctaaagcgg ccgcatgcgt gcgacctcgg ttgtccgttg cgacgccggc gcgctgattt
      121 ttggctagct cgcggcacaa taggccgcgc agaaattttt ctgaactgcc caactgatcc
      181 gagccattgc cgcgccgcga cgattgtaac gtcggcaatc cgcggcgaac gagaaacaag
      241 gtgacggcga tgggcgtgaa agaagtgccg agcgatcacg atttcttcga tccggcctac
      301 gtcaagcact ggaccgagag cattagcaga tatcggccgg agcgaaagcg actgttcgag
      361 gtatttgccg ccgaagccgc gcgcatcaag aacggcgcac tatcgatctt cgagctcggc
      421 tgcggaccgg gatttctcgc cgaggcactg ctgaacaatt gcccgaatgc gcgctacacg
      481 ctggtcgact tctcgccgca gatgctcgac ctcagccgga gtcgcctggc aaaatttaaa
      541 gaccggacgg ttttcattca ggcggatttc aaaaaagccg attggatagt ggggattggc
      601 gcgggcttcg atgtcgtcgt ctcactccag gccgtgcacg agctgcgcca cgcgacccgc
      661 atcccgaagc tttacgctca gctccacagc ctgctcgctc ctggaggaac cattttgatt
      721 tgcgatcacg tcaattcgtc ctccagtcat cgagccgccc atttcatgac ggtcgaagag
      781 catttgtcga ccttcaagaa ggctggtttc atcaacgcca gagaaatctg cgccgccgcc
      841 gacttgagcc tgatggcggc ggagaagccg taagaaaaag ccgaagcgtc cgcctgtcgc
      901 tcgaggcagg ctgccgtttg acacacggaa agcgcttctg cgaagatcgc gcaatctata
      961 aggaggaccc gacaaatgga gaataccaag accgatccta ttgtgatcga gcaaccaggg
     1021 atgcgcatga ccattgagca ccgcagcggc ctcggcggca acgatgaagg attgacgttc
     1081 gatgtcacga cagccgaatc agcggacgcg ggcaagagaa tcctgcgctt cgattgcttc
     1141 tacaagaatc ctcactatca cgtcggcgcc tcgggtgggg aagccgctgc ctacaaaatg
     1201 aaagacgaag gcatcgacga cccggtccgc tggacactgg aacagctcaa gacccgcttc
     1261 cccgctatgg tcagacaagc tggctacgaa gaaatcgcca ggacgatcga tcagcaggcg
     1321 attaccgatc agctcacacg actggagcaa aagataatcg acaagtacta atcgtcctga
     1381 acgatcgggt cgcgtcttgc attgcgacaa tgtgcgattg cgagacccgg cccgatcgaa
     1441 tggcgccacg atcacgcaca cgagcgttct tctgggaaaa caacaccgag gttctgaggg
     1501 gtcgaccggg atggtgccat attctcatca gaatacgtgg ctctgtaagc agacgagacc
     1561 tgaggatgat caatctcgat ctatggaaag gagtgacggg gtcatgtctt gcattgacac
     1621 atcttttgct tagtcgcgta ccgaaccggc gcattagtcg gcatgtctga gaaactttcc
     1681 gcgtgttagc tttacggcgt ctgatcggac aactcatgtg gttcgcttga aagcgcggca
     1741 acggctgtca cgcatcagtc atagagcttg ttgataaacc cggattgatc gagatccttg
     1801 acgatgctcg aatctacgaa tgagttggga tcggcccctt tggccttggc attgtccttg
     1861 ccgagaaact ccaagacagt tttgacgccc ggaatggatg ggtagggcac tttgttatag
     1921 taggttgaag ccaatagatc gtaagatttt tccagatatt tttcttgcgc aggctcggtg
     1981 cgaagtttct tcttgagaac ttcgagactc tccttcttat tccttttgaa ataggcgatt
     2041 ccttcaacat atccttttac aaagcgggtc gcgggatcat tgtgacttcg gagatgacta
     2101 cgcgtaaccg ctaacgcgtt ttgcaaatag taaatatcca tatcgaccaa atcggcgagg
     2161 acgttgtacc ccttttcctc cgccatgaac accgtcggct cggtaaagac tccgccgtcg
     2221 attgtccctt tatcgagagc gatcagcatt gacggtgaag actcccaggt gaccagttgc
     2281 actttatcta tgccgatatt ccaccgctgc agtaataact ggagaacggt atgagaggca
     2341 gagccaaaac caacgatccc aattttttgg ccgataatct ctttggggtt ctttatggga
     2401 ggacgagcga tcagtttctg cacgccctta ttcaatatcg atgcgaccat gacgacatcg
     2461 gcgccggcca atgaagcgct gattgccgcg gcgccgcctg cggttgcaaa ctgaatgtcg
     2521 ccgccgacca tcgcgctgat ggcgcgtgcg gcgccgccgc caacgttggt gacattgacg
     2581 tcgatcccgt gttttttgaa taaaccgcgt tcttcggcaa cccaaatggc gccgttaacg
     2641 gaactcgtcg aactcagtcc gacggtcacc cggtccaaag caaacgtgag ttggcaaata
     2701 tcaatgacgg acagaggaat tagaattaaa cccgctgcga gttttttaag cgccatgcgc
     2761 cctccgcatc gacagtgccg ttatttgccc ggttgcgcgg gatcgtagca aacgtgcgaa
     2821 aacttgaaaa gccgctttaa atcgcaaagc cacagtggcg tgtcgtttcg cgagttggct
     2881 cagcactttt ttcacgatct ccgtcaggga gcgccaacgc cgcaatccta tctccagaag
     2941 tgtgattcaa gttggatcac gggatgtatt tttcatcttg caagaaaagc tctgttggga
     3001 tttcagttcc taagcccaat tccttgcagc gatgataaat aagatggcca acagcggtaa
     3061 actgcacgcc gtcgccacgc aagacaagcc ctgtgatctg gcggtcggaa gtgcgtcctg
     3121 ggtggttccc cgcgatcaca tcgcccaaca gtatgacctt atcccacttg atacgcgtcg
     3181 gttgcgtata ttgatccttg gactcttctg ggatcgcttc cactccgccg atcgcatacc
     3241 tcatccaacg cctgccgtgg cgattcatcg tcagcacatc
//
LOCUS       DATLCH010000231         5554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5839030, whole genome shotgun sequence.
ACCESSION   DATLCH010000231 DATLCH010000000
VERSION     DATLCH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5839030"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VMZ02_06780"
     CDS             <1..599
                     /locus_tag="VMZ02_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71672.1"
                     /translation="VTSLVVLVLVLCLDGYLSSIERVTVAKIIGEPWITLSMLATWAG
                     MWALASRLVVNRYNFAEHVTIACLALLTISALGLFAEWSEFFLPRIPMLWIAGLFGYG
                     LVLAGLVYGHLGAASALRRRSRLSAAAAISLVVVGTSAISDFAARSNFSTIMEYSGVL
                     KPIDASWVPVTSVDDFVGSSEKLKKELDRLTKKAIAQQ"
     gene            716..1720
                     /locus_tag="VMZ02_06785"
     CDS             716..1720
                     /locus_tag="VMZ02_06785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR71673.1"
                     /translation="MIYDVEINSAAHYPAAEVPGLIELAEQAGFGAFWKGESNSTDPF
                     VMLAAVASRTKKIKLGTAIYHIYGRSPVTLGIQSATLQDLSGGRLLLGLGVANKAIAG
                     WHGGVFDRPLKRAREYIEVTRKVAAGERVEYEGEIYNTGKRFQLSWKPSFPNVPVYLA
                     GLGPQMTKLVGKIADGVVINMATPAKVREIAARVREGAVEAGRDPSKIEIIAKSRVSL
                     NADKNAARAKLRQVLTFYNIADHYSDMLRGMGFDKEVSAIHDAFQKGGFKAAMGALTD
                     EYMDKLPVVPTSDVREIKEKMKAFEEAGVTRMVIPYVPVTEPVVDDARRFFEAWGNSA
                     "
     gene            complement(2197..3228)
                     /locus_tag="VMZ02_06790"
     CDS             complement(2197..3228)
                     /locus_tag="VMZ02_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71674.1"
                     /translation="MKTCAARIAGRTIRSAIFIIFALTCTQLPTRLTAQTRSYLAGSV
                     TSESMTALWVARERGLFKKHGLDMQYILMPRNPLAIAALLAGEIDAAIVGPGHVINAA
                     SGGADAVGIANLSQKLDYRLIARPEIKSKEDLRGKRIAISGPGSTSHLVTMLALQGLG
                     VDATQARIAYLTIPGTEMNRRLALETNGVDATGLRGAVGDLYGNKGYTVLYNFKTTGV
                     TLPQSMLTTTRRIAAAKPQVIDAYLKTMIEAIAVTVDPANREMVTRLLASNLRLSPAD
                     AEESYQAVINFYERVPHTNVEGMRRLHKLLLQINPKMGDVRVENTIDNSFLSKLESSG
                     YIQSVYKKN"
     gene            complement(3238..3630)
                     /locus_tag="VMZ02_06795"
     CDS             complement(3238..3630)
                     /locus_tag="VMZ02_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71675.1"
                     /translation="MGKLRHLGIRTEDTGKLASFYVDVFDMKVIHKSKEEGGTIFLSD
                     GYFNLAIISNKDQNSPNGLYHFGFEVENGEAIVEKMKKVNPNKLPKPRPNGRPYAETR
                     GSDPDGNYFDISEHGFLDVQPVGEKNPS"
     gene            complement(3862..4065)
                     /locus_tag="VMZ02_06800"
     CDS             complement(3862..4065)
                     /locus_tag="VMZ02_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71676.1"
                     /translation="MLARLIFCGALCVISGCATERTVMINSRGEEAVCETAGSGFFGA
                     ISVNHQQQQCIADAEKRGYGVKK"
     gene            complement(4188..5141)
                     /locus_tag="VMZ02_06805"
     CDS             complement(4188..5141)
                     /locus_tag="VMZ02_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71677.1"
                     /translation="MKNILLSAFFALALTVTGAFSTAHAEVVNIGISTVGLYELPTEI
                     SKRKGFYQEEGLDVRKIVVRSPLHVPALLAGELDYSTITGLILGASVQGMPLKSVMGW
                     FDRPLHMLIARANIKRLTDLKDKRVAVSGFGSIPHVMLREALAGAGMNPDKDIIVLAL
                     GGSGDRLAALAAGIVDATPLDVAYIQRTEKLGYNNLLYLGDVVNLRLGGFAVSNDKIQ
                     RNPEQITRVVRATLKGVRFMKINKPETMAIMRDYLKISGDYVEKIYLFALKSLNEDGL
                     VAKNSLDNEIRLAREQLKVKDEVPESRVAEWKFIKDVLAKR"
     gene            complement(5333..>5554)
                     /locus_tag="VMZ02_06810"
     CDS             complement(5333..>5554)
                     /locus_tag="VMZ02_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71678.1"
                     /translation="YKRMENPRIVPLAWFRSFLEEQEEIFGSDPWIYGLGEANRKNLE
                     TLMQYSHEQGLIGRKMALGELFNDPTALR"
BASE COUNT         1169 a         1478 c         1617 g         1290 t
ORIGIN      
        1 ccgtcacttc gctcgtggtt ttggtgctgg tgctctgttt ggatggttat ctaagcagta
       61 tcgagcgcgt tactgtggcc aaaataatcg gcgagccgtg gatcactctc tccatgctgg
      121 caacctgggc gggcatgtgg gcgctcgcca gccggcttgt cgtcaaccgt tacaattttg
      181 ccgagcatgt aacgatcgcc tgcctcgctt tgttaacgat cagcgctctc ggtctgtttg
      241 ctgagtggtc tgagtttttc ttgccacgga ttcccatgct ctggatcgcc ggtttatttg
      301 gttatggtct ggtgctcgca ggactggtgt acggtcatct gggcgccgcc tcggcgttgc
      361 gccgccgttc ccgactttcg gccgcggcgg cgattagtct ggttgtcgtg ggtaccagcg
      421 cgattagcga tttcgcggcg cgcagtaact tctcgacgat catggaatat agcggcgtgt
      481 tgaaaccgat cgatgcgtct tgggtgccgg tgacctcggt cgatgatttc gtcggcagtt
      541 cggagaaact gaaaaaagag ctggatcgat tgactaagaa agctatagcg cagcagtgag
      601 ttcaaggccc gacaagattt gacataagtg aacccatctg gctagtgtac ccggtaattt
      661 cgttcagggt taagcgccta aaaaaaattt aaagaatacg ttaggacggg atcttatgat
      721 ttatgacgtc gagatcaatt ccgcggcgca ttatccagcg gcggaagtgc cggggctcat
      781 cgagttggcc gagcaagcag gtttcggcgc cttttggaaa ggcgaatcga atagcaccga
      841 tccgtttgtc atgctcgcgg cagtggcgag ccggaccaag aagataaaac tgggcacggc
      901 gatttaccac atttacggcc gcagcccggt aacacttggg attcaatcgg cgaccctgca
      961 agatttatcc ggcggccgtt tgctgctcgg tcttggcgta gccaataagg cgattgccgg
     1021 ctggcatggg ggcgtcttcg accggccgct caaacgagcg cgtgaatata ttgaagtgac
     1081 gcggaaagtc gcagccggtg agcgcgtcga atacgagggc gagatttaca atacgggcaa
     1141 gcgctttcaa ctctcctgga agccgagttt tcccaatgtg ccggtttatc tcgccggctt
     1201 aggcccgcag atgaccaagc tcgtcggcaa gatcgccgat ggcgtggtga tcaacatggc
     1261 gacgccagcc aaggtgcgtg agatcgccgc gcgggtgcgc gagggcgccg tcgaagccgg
     1321 ccgtgatccg agcaagatcg aaatcatcgc caagtcgcgc gtctcgctca atgccgataa
     1381 gaatgccgca cgcgccaagc tgcgccaggt gctgaccttc tacaatatcg ccgatcatta
     1441 cagcgacatg ctgcgtggca tgggtttcga caaggaagta agcgcgattc acgacgcgtt
     1501 tcaaaagggc ggcttcaaag ccgccatggg cgcgctcacc gacgagtaca tggacaagtt
     1561 gccggtcgtg ccgaccagcg atgtgcgcga gatcaaagaa aagatgaaag cgttcgaaga
     1621 ggccggcgtc acccggatgg tcattcccta cgtgccggtg accgaaccgg tggtcgacga
     1681 tgcccggcgc ttttttgagg catggggaaa tagcgcctag cggcgctgtt caacgttcaa
     1741 gggactggcc ggcgccctga actttgaact tttgaacctt gaactttgaa cgaataattc
     1801 tacggaggaa aaatttctat gtgtgatttt gctgcggacg ggaaggctgc cgaagaactg
     1861 atgctcgggc ggacgttgac ggtatcgcgc gcgcgagctt aatgccaagg attactttta
     1921 tcagatgctc taggacaacc cggagatgct gaagatctat ccgggtatcg agaatgagct
     1981 gctcgacggc gccatcgatg tgcttacaga gcggttagat aatgtccctt tcggaatggg
     2041 gggaaatccc gctttcaggc gaggaaagga tttgccatgc cggcgaaggc cggcatccat
     2101 gcttcggttt cgttagtagg ccacgaaaac ctggattccg gcctacgccg gaatgacgga
     2161 aggaagagtc gacttctagt tggagacgtg agtcgttcag ttcttcttgt aaacgctctg
     2221 gatatacccg gaactttcga gcttgctcag gaaggaatta tcgatggtat tttcgacgcg
     2281 cacatcgccc attttcggat tgatttgcag caacagcttg tgcagccgcc gcatgccctc
     2341 aacgttagta tgcggcaccc gctcatagaa atttatcacc gcctgataag attcttccgc
     2401 gtcggccggg cttaagcgca gattcgatgc cagcaggcgg gtgaccatct ccctgttggc
     2461 gggatcgacg gtaacggcga tcgcttcaat catggttttc aagtaggcat cgatcacctg
     2521 gggcttggcc gccgcgatgc gccgcgtggt ggtcagcata ctttggggca gtgtgacgcc
     2581 ggtggttttg aagttgtaaa gcacggtgta gcccttgttg ccgtagaggt ctccgaccgc
     2641 gcctctcaga ccggtggcat ccaccccgtt ggtttccaac gccaagcgcc ggttcatttc
     2701 agttccagga atagttaaat aggcgattcg cgcttgcgtg gcatcgacgc cgaggccctg
     2761 cagcgctagc atcgtcacca gatgcgacgt cgagccggga ccgctgatgg cgatgcgctt
     2821 gccgcgcaaa tcttccttgg attttatttc cggtctggcg atcagacgat agtcaagctt
     2881 ctgtgacagg ttggcgatgc cgacggcgtc ggcaccgccg ctggcggcgt tgatcacgtg
     2941 gcccgggccg acaatggcgg catcgatttc gcccgctagc aacgcggcaa tcgccaacgg
     3001 gttacgcggc atgagaatat actgcatatc caagccgtgc ttcttgaaca aaccccgttc
     3061 ccgcgcaacc caaagagccg tcatgctttc gctggtaacg ctgcccgcca agtaactccg
     3121 cgtctgcgcc gtcaagcggg ttggcagctg cgtgcacgtg agagcgaaaa tgatgaaaat
     3181 tgccgaccgg atcgtccggc cggcgattct cgccgcgcaa gttttcaccg tcgttgctta
     3241 gcttgggttt ttctcgccaa ccggttgtac atcgagaaaa ccatgctcgg agatgtcgaa
     3301 atagttgccg tcgggatccg atccgcgggt ctccgcatac ggtcttccat tgggccgcgg
     3361 cttaggcaat ttgtttggat tgaccttctt cattttttct acaatggcct caccgttttc
     3421 gacctcgaag ccaaagtgat aaaggccgtt gggcgaattt tgatctttgt tcgagatgat
     3481 cgctaggttg aaataaccat cggaaaggaa gatggttccg ccttcctctt tactcttgtg
     3541 gatcaccttc atgtcgaaga cgtcgacata aaacgacgcc aacttgccgg tgtcttcggt
     3601 gcgaatacct agatgccgaa gttttcccat ggtgccctcc ttgtgatcaa gtaaacgttg
     3661 atcttggtcg cccagttaac gtgagcgaac gctataagat cgttcgttgc cttgtcaagg
     3721 cgatatcgtc gatgtgactt tggcgtgcag aatgcacacc actttcatgg ctactggcaa
     3781 acgacaggga aagaaaatga atcgagggca gcgctaaaga atacaatccg cggaagcgct
     3841 tcctatgtgc cacgctgaag gctacttttt gactccgtag ccgcgcttct cggcgtcggc
     3901 gatacactgt tgctgctgat gattaaccga tattgcgccg aaaaagccgg agccggcggt
     3961 ttcgcagacg gcctcctcgc cgcggctgtt gatcatcacg gtccgttcgg tagcgcagcc
     4021 ggatatcacg caaagagcgc cacaaaagat caatcgagca agcatgtttt tagtatagcg
     4081 ctaagcggat tgtcggcaat gggttctttt ttgacgcaag gtccgggaga gcaaagaacg
     4141 gaacgcgctg acgcacccga ctgcaacaag tagaaggcgg ccaatattta tcgtttggcg
     4201 agaacgtcct tgataaattt ccactctgcg accctggatt cggggacttc gtctttaact
     4261 ttgagctgtt cccgcgccag gcgaatctcg ttgtccaaac tgttcttcgc gaccaacccg
     4321 tcctcgttga gagatttcag cgcgaacagg tagattttct cgacgtagtc gccgctgatt
     4381 ttaaggtaat cgcgcataat cgccatggtc tcgggcttgt tgattttcat gaagcgcacg
     4441 cctttcagcg ttgcccggac gacacgggtg atttgctcgg gattgcgctg aattttatcg
     4501 ttactgacgg cgaagccgcc gaggcggagg tttacgacgt cgccgagata gagcagattg
     4561 ttatagccga gcttttcggt gcgctggatg tatgccacgt cgagcggcgt agcatcgacg
     4621 ataccggccg ccaacgccgc taggcgatcg cccgagccgc cgagggcgag cacgataatg
     4681 tctttgtctg gattcatgcc ggcgccggca agagcctcgc gaagcatcac atggggaatc
     4741 gagccaaagc cgctgacggc gacgcgcttg tccttgagat cggtgagccg ctttatattg
     4801 gcgcgggcga tcaacatgtg cagcggtctg tcgaaccagc ccatcaccga tttgagcggc
     4861 atgccctgga cgctcgcgcc caagataagc ccggtgatgg tggaatagtc caactcgccg
     4921 gcgagcaacg ccgggacgtg cagcggcgac cgcacgacga tcttgcgcac atcgagccct
     4981 tcttcctggt aaaagccctt gcgcttggaa atttccgtcg gcagctcgta aagcccgaca
     5041 gtggaaatgc cgatgttgac gacctcggcg tgggcggtgc taaaggcgcc ggtgacggtc
     5101 agcgcgagag caaagaacgc ggacaagagt atgtttttca tttcttagtt tttccgctga
     5161 aatgttgtcg actgcagttt attcgcgggt cgttccaatg tgcgcgacct attctcgact
     5221 cgttaattat tcaccgagac gtcgcgcaac tcgccagtcg cgcacgacac cgcaataacg
     5281 gataaacatc tggcaattgg gccatcacgg actgtgccgt ctctgctttg gattatcgca
     5341 gcgccgtcgg atcattaaac aactctccta gcgccatctt tctgccgatg agcccttgct
     5401 cgtgagagta ctgcatcaat gtctcgaggt tcttgcgatt ggcctcgccg aggccgtaga
     5461 tccatgggtc gctgccgaag atttcttcct gctcctcgag aaaagagcgg aaccacgcca
     5521 acggaacgat gcgcgggttc tccatacgtt tgta
//
LOCUS       DATLCH010000232         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3310034, whole genome shotgun sequence.
ACCESSION   DATLCH010000232 DATLCH010000000
VERSION     DATLCH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3310034"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..219)
                     /locus_tag="VMZ02_06815"
     CDS             complement(88..219)
                     /locus_tag="VMZ02_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71679.1"
                     /translation="MIDDLILLALVVVLVPLAAIVTFRLLGKTKRTRRPLNSFRIER"
     gene            complement(435..854)
                     /locus_tag="VMZ02_06820"
     CDS             complement(435..854)
                     /locus_tag="VMZ02_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HUR71680.1"
                     /translation="MATLISAEKEKQLAERMISLGVREADIDEQFVRSSGAGGQNVNK
                     VASCVVLHHRPTGIRVKCQQERSQALNRFLARRLLLDKIDLQQRGAASAEAQRIAKIR
                     RQKRKRSHRAKMRMLDDKRHQSEKKGRRSIVRPGPDE"
     gene            complement(875..1957)
                     /locus_tag="VMZ02_06825"
     CDS             complement(875..1957)
                     /locus_tag="VMZ02_06825"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71681.1"
                     /translation="MNEQTAVIDADAHVVETERVWDYLEGAEKKFRPTLMTSPENPKR
                     QIWVLDGENLGSKFPSPDEQESQAHLKRFGREVATPVAARELSNVKQRLKHMDELGID
                     VQVLFNSLWITPLTSRADVEIALSWAWNRWLADVWKMGEDRLRWTCVVPALSPHEAVA
                     QIRFAKEHGAVGVCMRPFERDRIMTDPFYYPIYEEAQRLDIPVTVHLANGNPELFRIM
                     TNEPGGGFSTFRVPTVTSCYALIMSEIPQVFPKLRWGFIEVSSQWVPWVIHEAIRRSL
                     GSARPISRDCFRDYHIYVSTQSDDDFTYILSYAGEGNLVIGTDYGHGDTSSELNAIKR
                     FQALTELSSQAKRKILFDNPKALYGL"
     gene            2129..>2801
                     /locus_tag="VMZ02_06830"
     CDS             2129..>2801
                     /locus_tag="VMZ02_06830"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HUR71682.1"
                     /translation="MNEFANLRQQMGGILTALAVGIPAGFLFAWMHTPIPWMLGPMIA
                     VASLNLIGVTMHSPPYARQLGQVILGSAVSLYFTPPVVAALGGNLPAILAATVAVFIV
                     GIFGALTLSKASGVDWKSTFFASIPGGAMAMAVLADRYGAQMSPVAVAHSLRVSVVVI
                     IIPLVLTYGGIPLEAAAYKPNLPLVPAILAIWLTVSYFLGELSERWHFHNGCLLVPIF
                     FGAALT"
BASE COUNT          622 a          780 c          733 g          666 t
ORIGIN      
        1 tcaagacctg ctcgagcagc tgaaaaagag caaatagaca ttcgtccaat aggacgatca
       61 agaaacttac gcgtcgttat ggcgaggtta cctctcgata cggaaactgt tgagcggtct
      121 tctcgttctt ttggttttac cgagtaggcg aaacgtaaca attgccgcca gcggcaccag
      181 caccaccacc aatgccaata gaatcaagtc atcaatcatg tcgcacctct cctcgcgctg
      241 aataacagca actaaaaggc catacaccgg attattaaat ttcaattagt taaagaaatt
      301 gctgacaacc aagctgctgt caaaagtaga caaatatgac aaggctgtca aatggaatga
      361 cgatcgtgcg ccgacatgcg aaacaatgcg ccgcctatga attcttcagc agcgtaaaca
      421 tacggagtaa aaatttattc atcgggaccc ggccgcacga tcgacctgcg gcccttcttc
      481 tccgactggt ggcgcttgtc atcgagcatg cgcattttgg cgcggtgcga acgcttgcgt
      541 ttttgccggc gaatcttcgc gatacgttgc gcttcggcac tggcggcgcc gcgctgctgt
      601 aaatctattt tatcgagcaa gagtcgacgc gctaaaaagc gattgagcgc ttgggaacgc
      661 tcctgttggc atttaacccg gatccctgtc ggtcggtgat ggagtacgac gcaggatgca
      721 actttgttga cattctggcc gccggcgccc gaagaccgga caaactgctc atcgatatcg
      781 gcttcgcgca cgccaagaga gatcatgcgc tcagccagct gcttttcttt ttcggccgat
      841 attaatgtcg ccacggttac acaaattcgg aaagttataa cccgtaaagc gctttgggat
      901 tatcgaagag aattttgcgt ttggcctgtg agctcagttc cgtcaatgcc tggaaccgtt
      961 taatagcatt gagttcactg gaagtatccc catggccata gtcggtgccg atgaccaaat
     1021 tcccttctcc cgcgtaggac aaaatgtacg tgaaatcatc atcgctctgg gtcgacacat
     1081 agatatggta gtcgcggaaa caatcgcgcg aaatcggccg cgcactgccc agggaccgtc
     1141 ggatcgcctc atgaatcacc cagggaaccc attggctcga cacttcgata aagccccaac
     1201 ggagtttcgg aaagacttga gggatttcac tcatgatcaa tgcatagcag gaggttaccg
     1261 tcggcacgcg gaacgtcgaa aaaccgccgc cgggttcgtt ggtcataatc ctaaagagtt
     1321 ccgggttgcc gttagccaaa tgcaccgtca ctggaatatc aaggcgctgc gcctcctcgt
     1381 atatcggata gtaaaacgga tcggtcatga tccggtcgcg ttcgaagggt cgcatgcaca
     1441 cgcccactgc gccgtgttct ttggcgaagc gaatttgcgc caccgcctcg tggggcgata
     1501 acgccggcac cacgcaggtc caacgtaaac ggtcttcgcc catcttccaa acgtcagcca
     1561 accagcggtt ccacgcccag ctgagagcga tttcgacgtc ggcccggctg gtgagcggcg
     1621 tgatccacag ggagttgaac aacacttgaa cgtcgattcc aagctcgtcc atatgcttaa
     1681 gccgctgctt gacattggac agctcacggg ccgccaccgg cgttgccact tcacggccga
     1741 agcgtttgag atgcgcttgt gattcttgtt catccgggga aggaaatttg ctgccaagat
     1801 tctcaccgtc gagcacccaa atctgccgtt tgggattttc cggcgacgtc attaatgttg
     1861 gacgaaactt cttttcggcg ccctcgaggt aatcccaaac ccgttcagtc tcgaccacat
     1921 gggcatcggc atcgattacg gcggtttgct cgttcatctt cccagctcct gacgcaaatt
     1981 aactatccga cctgcaatcg tctgtccacc gcgggccaaa gtaaaatttc tcaaccccgt
     2041 cgtaatcaca ggacataccg gttataaccg ttaagctcag ctgacagcgc ttattactga
     2101 agttgtcagc gccaagccta acccaaccat gaacgagttc gccaacctgc gccaacaaat
     2161 gggcggcatt ctgacggcat tggcagttgg cattccggct ggctttctat tcgcctggat
     2221 gcacacaccg ataccatgga tgctcggtcc aatgattgcg gtggcgagcc tgaatctcat
     2281 cggcgtgaca atgcattcgc cgccctatgc gcgccagttg ggtcaggtga tcttgggctc
     2341 ggcggtgtcg ctttatttta cgccgccagt ggtggctgct ctcggcggca atctgcccgc
     2401 catattggcg gccaccgtgg cggtgtttat cgtcggcatc ttcggcgcgc tgactttaag
     2461 caaagcttcc ggtgtcgatt ggaagtcgac attttttgca tcaattccag gcggtgccat
     2521 ggccatggcc gtgctcgccg atcgctatgg cgcgcaaatg tcgccggtgg cggtggcgca
     2581 cagtctgcgc gtctcggtcg tcgttatcat cattccgctg gtgttgacct acggtggaat
     2641 cccattggaa gcggcggcct ataagcccaa cctaccgttg gttccggcga tcttggcgat
     2701 ttggctaacg gtcagctatt ttctcggcga gctttcggag cgctggcatt ttcataacgg
     2761 ttgcctgctt gtgccgattt tttttggtgc tgccctgacg a
//
LOCUS       DATLCH010000233        11710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9575925, whole genome shotgun sequence.
ACCESSION   DATLCH010000233 DATLCH010000000
VERSION     DATLCH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9575925"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VMZ02_06835"
     CDS             complement(<1..271)
                     /locus_tag="VMZ02_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71683.1"
                     /translation="MDCLKKLFRPSATAAFCALLLLSLGDPVWPSHHYTAKQMEALAS
                     RVGQTFWSKPVDGKGPSFVAAPAANAKPIRVDAIESLQITALVGQA"
     gene            complement(460..536)
                     /locus_tag="VMZ02_06840"
     tRNA            complement(460..536)
                     /locus_tag="VMZ02_06840"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(500..502),aa:Met,seq:cat)
     gene            598..1020
                     /locus_tag="VMZ02_06845"
     CDS             598..1020
                     /locus_tag="VMZ02_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HUR71684.1"
                     /translation="MSQKFRNPFPTVDIIIEIDDGIVLIERKNPPPGWALPGGFVDYG
                     ETVERAAVREAKEETCLDVRLTEQFYTYSDPQRDPRHHTLAVVFIATAQGTPRGADDA
                     RVAEIFYEGGLPSPIAFDHSAILRDYFHFKKTGQRPKP"
     gene            complement(1017..2213)
                     /locus_tag="VMZ02_06850"
     CDS             complement(1017..2213)
                     /locus_tag="VMZ02_06850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HUR71685.1"
                     /translation="MTFLAERTKLIKPSVTLAIAAKAGKLRSEGIDIVNFSAGEPDFD
                     TPEHIKDAAIEALRRGMTKYTDVKGIEPLREAIVQKYQKEYGLSYRKEDVVVSCGAKH
                     SLYNLLQAAVNPGDEVLIPAPYWVSYADMALLAGGVPKLIPTDESTGFRIRPEQLQAG
                     LTAKTKVFFLNSPCNPTGATYSKDELLAIAAILKKHSCLIFADDIYEKIIYDGFEMHN
                     IVALCGDLRERTIIINGVSKTYAMTGWRIGYAIGASDAIAAAGKIQSQSTSNPTSIAQ
                     WAALEALRGGQESVMMMVREFQKRRDVIVERLNTIPGIRCLKPEGAFYVFPNIAGLIG
                     KSANGKKLTSPCDVADYLLDEAKVAGVPGEDFGSQRHIRFSYATSLADIEKGCRRIAD
                     AVAQLT"
     gene            complement(2227..2718)
                     /gene="coaD"
                     /locus_tag="VMZ02_06855"
     CDS             complement(2227..2718)
                     /gene="coaD"
                     /locus_tag="VMZ02_06855"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HUR71686.1"
                     /translation="MANNDLAIYPGSFDPITNGHVDLVNRTLRVFDKVVIAIATNPDK
                     DRCLFTVEERLQLINEVFARADGRVSADSFQGLLVNYAERRGATVVIRGLRAISDFEY
                     EFQMAMMNHRLKPKLETFFMMTGESEFYTSSRLVKEVVSLGGNVAGLVPDNVLKKLKE
                     KFK"
     gene            complement(2744..3301)
                     /gene="rsmD"
                     /locus_tag="VMZ02_06860"
     CDS             complement(2744..3301)
                     /gene="rsmD"
                     /locus_tag="VMZ02_06860"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HUR71687.1"
                     /translation="MKVIGGAARGRKLKVPKGLAVRPTAARVKESLFNILQHDFSGLR
                     VLDLFAGSGNVSIEALSRGALAAVLVDESARTAAVIRDNLMKLDLSSRAQVWIAPVRR
                     SLRKLDKAGEKFDLIFLDPPYEKGLVATTINDIAKSSLLSPAGVVVVEHSGRETVKPS
                     YGDLVLNDQRRYGDTCLSFFQVRKS"
     gene            complement(3324..4364)
                     /locus_tag="VMZ02_06865"
     CDS             complement(3324..4364)
                     /locus_tag="VMZ02_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUR71688.1"
                     /translation="MKAIRIHHHGGVEQLRYEETNDPILQSPEYVIVRLKAAALNLAD
                     IQVREGITDSKRLCLPRIPGSDGAGVIVALGSAVKELKIGDAVCFYPQSGCTTCWACR
                     SDQEHLCEQPRRLGEQVDGTYAQYLQVHKQNCFKIPTTLAYEEAAAIPVAYLSAWRML
                     LTNAALKPGESVLIRGIGDCVATAALQLAVGFGAYTIVTADTTEKLSQALRLGAQHGI
                     NEGDHDIAAEVRRVTGKRGLDVVVDCVGGGSWANSVATLNRDGRLVTCGASAGAQPLT
                     DLRRIFWHHLKVFGSTLGTRGELSRLIDFFESTGTKPVIDSFYDLADAGQAQQRLAKG
                     RYFGKIVLRTNS"
     gene            complement(4475..4822)
                     /locus_tag="VMZ02_06870"
     CDS             complement(4475..4822)
                     /locus_tag="VMZ02_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HUR71689.1"
                     /translation="MVNPQSCLDLKLSNGAIEQIKKLLTRDNRADHGLRVSVTDGGCS
                     GHSYKLDFEREQKAGDTVLDQQGLKIFVDAASVPFLQGMIIDYESGLYGGGFKFSNPN
                     ATATCGCGTSFSA"
     gene            complement(4926..5852)
                     /gene="ftsY"
                     /locus_tag="VMZ02_06875"
     CDS             complement(4926..5852)
                     /gene="ftsY"
                     /locus_tag="VMZ02_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HUR71690.1"
                     /translation="MAVGAFFQRLKEGLSKSRETWVQKIGTIFQNRQWDEKSLDEMEE
                     SLLTADVGVKATDKLMQALRRQSPGDIEDLSQDMMAKLQAAMIDILRGSVAPPKRAPL
                     SVRPWVIIFLGVNGVGKTTTIGKLAAQYRADGKKVLLVAGDTFRAAAIEQLDAWGKRV
                     GVDVVKHKAGADPSAVVFDGMQAAKSRNVDVLLIDTAGRLHTKVHLVDELKKIRRVIT
                     RELPDAPHETLLVLDATTGQNGLQQARVFKEATDISGIVLTKLDGTAKGGVIIGIYEE
                     LGVPVQFIGVGEDVEDLQPFEPESFVEALFEK"
     gene            complement(5927..9481)
                     /gene="smc"
                     /locus_tag="VMZ02_06880"
     CDS             complement(5927..9481)
                     /gene="smc"
                     /locus_tag="VMZ02_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HUR71691.1"
                     /translation="MRLKRLEMVGFKSFAEKTFIEFNPGVTAVVGPNGCGKSNIVDAL
                     RWAMGEQSARHLRGHQMEDVVFAGSDTLPPTGMAEVSITFDNEEGRSPAEYSHLAEIM
                     VTRRLFRSGESEYAINKTTCRLKDVVELFLGSGVGSKAYSIVEQGKVDELVNSKPEER
                     RGLIEEAAGTSMYRSRKAVAEKKLERTQQNLLRVSDIVREVERQIRTMELQAKKAERY
                     RALRSELREKDFAFTTLQRDALHDDIAQQEAQLAKVEDRLAEHLAMLHSKEAENESVR
                     LSLLEADKAIGAQQEIVFQQRTQIQTDEQKCDFYRRDLEQLEQAENEAKIALATITER
                     LKTLLQEIDELTKAKESFIQLSLFEESFLRERETELSGLQAQIHALQDNIDREKTALI
                     DGANQIAYLKNDNLAKDKRQGEIAAELSRCENESAQATLSLTTCTERRQETGQALNTC
                     VEELRERALESASVSASIQTLSRVREEQQNRIEALKSEIQENRSRLLSLEDLQRNYEG
                     YHEGVRAIMLKKQQAASPNGIYGLVAEVIEAPESYEKALTAVLGDRLQYVIVKGHEEG
                     VESIEFLKQEASGRGSFIPLQLRHRQEKALPLNEAEVIAPLLGLVSIKDGYREIAEYL
                     LSDVIVVPNLAAGLALWNRNGYYCTIVTPDGEVIDPMGTVTGGSTTPLEGNLLTQRRR
                     IRELGDTLANLAAKLPHEETEFEKLRAELEQAETRKTILTNEIHRLELARVRLEHETR
                     AAGDEYERLSRTLEALGQEQQDLAAALQSLRDESQRNYDMIQVRTADRLAGEQSLAEK
                     QARFGELRGSVESAESAVTQSRIRNAALGEKRENTHANLNNRLKQRAETEQEIASWQA
                     RGTDCQRQRSEIAEALREVEVSLCGAKEELQALESRLQADRQSYRTISMQLAEIGETI
                     KELRPLGEACQEERSRIQLTLAEKRLNLQHLADNLREKYDTDLDSLSAETGDIPPSRD
                     QLLLEIEDLRGRLERMGEVNLAAIGEYEELTSRFQFMGRQKEDLEKSIADLQQTIVKL
                     NRICRLRFKESFEAINEKFEMIFPRLFRGGKAKLVLTDENDFLETGVDIIVQPPGKKL
                     QSITLLSGGEKALTAVSLLFAIFLTKPSPFCFLDEVDAPLDDANLDRFNEMIREMSSH
                     SQFVLVTHNKKTMESAEMLYGITMAEPGVSKLVSVRMS"
     gene            complement(9702..10334)
                     /locus_tag="VMZ02_06885"
     CDS             complement(9702..10334)
                     /locus_tag="VMZ02_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HUR71692.1"
                     /translation="MSCSATAPGYSILHTKVLILNRSYLPIHVTSVKRAFVLLYQGIA
                     KAVNEQYQTFDFDSWSDLSISAHDETVGMVNRVIRVPRVILLVAYDRVPKRQVRFSRF
                     NIYARDKLTCQYCHRRLPRQELNLDHVIPRSQGGVSTWENVVCSCHECNRRKGGRTPH
                     QAGMLLARAPLRPQWTPFMQETFSLSRYKEWTPFLNTVDASYWNTELLQN"
     gene            10579..>11710
                     /locus_tag="VMZ02_06890"
     CDS             10579..>11710
                     /locus_tag="VMZ02_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HUR71693.1"
                     /translation="MKQEIIINSTPQESRIAIVEDGLLAEFFVERKEEMGVAGNIYKG
                     KVARVLPGMQAAFIEIGMDKAGFLHASDFSTPDDVHVISPANDDVEFEEAPPKSNPSR
                     YLPLEKQLSRGEEVLVQVAKDPLGTKGARVTSHISLPGRYMVFMPGNKHIGISRRIES
                     DEERKRLKEIAQSLGTDEGGFILRTACEGLSKREIQRDLGFLQKLWKSIQKKAETAPA
                     PSLIHQDLDLIARAIRDYFSADTEQVLIDSPKDHRRIVDFVRHFMPRLKSKINLYSDK
                     EPLFEQLGIEEKITKALVPRAWLRSGGYIIIERTEALTAVDVNTGRFVGKRNQEETIL
                     KTNLEAVQEVVRQLRLRNAGGLIIIDFIDMDKEANRKKVYEAL"
BASE COUNT         2536 a         3143 c         3223 g         2808 t
ORIGIN      
        1 cggcttgtcc caccagcgcc gtaatctgaa gcgattcgat cgcgtcgaca cgaatcggct
       61 tggcattagc cgccggagca gcgacaaaac tgggaccttt gccgtccacc ggtttactcc
      121 aaaatgtctg gccgacccgg cttgccagcg cttccatctg cttggcggta tagtgatgtg
      181 acggccaaac gggatcgccc aagctgagca gcagcaacgc acaaaaagcc gcggtggcag
      241 aagggcgaaa tagttttttt aagcaatcca ttgaaccggg ttacttcttc tactttgacg
      301 acgccggagg aaaaggcttt cgctgagaaa tttatcagct ccgcgcggcg atccaaatct
      361 atacggaggt tatgaaaaag tccaaaccga cagaaggagt catgcggaga tgcagtgatt
      421 gggcaaataa aaaggggccg ttaggcccct taagtatctt ggttgcgggg ccgggatttg
      481 aacccgggac ctcaaggtta tgagccttgc gagctgccag actgctccac cccgcgataa
      541 aactatcgag tgctctttct accttgcggt ttccgtgtag tcaaggtagg gaagcgcatg
      601 agtcaaaaat tccgcaatcc ttttccgact gtagacatta tcatcgagat cgatgatggg
      661 atcgttctga tcgagcgaaa aaatccgccg ccgggctggg cgcttcctgg cggattcgtc
      721 gattacggtg agaccgtgga gcgcgcagcg gtgcgtgaag ccaaggaaga aacctgttta
      781 gatgtgcgat tgaccgagca gttctacacg tactccgatc cacagcgcga cccgcggcat
      841 cacactttag cagtggtgtt tatcgcgacg gcacagggca caccgcgggg ggccgacgac
      901 gcgcgagtgg cagaaatttt ttacgaaggc ggtttgccaa gcccgatcgc cttcgaccac
      961 tcggccattc tgcgggatta cttccacttc aagaaaaccg gccagcggcc gaagccttaa
     1021 gtgagttgcg ccaccgcatc ggcgatacgc ctgcagccct tttcgatatc ggccagggat
     1081 gtcgcgtagg agaatcggat gtgccgttgg gagccgaaat cttctccagg aacgccagcg
     1141 actttagctt cgtcgagtag gtaatcggcg acgtcgcacg gcgaagtcag tttcttacca
     1201 ttagccgact taccgatcag acccgcgata ttgggaaaga cgtagaaggc tccttcaggt
     1261 ttgaggcaac gaattcctgg gatggtgttg agacgctcga caatcacgtc gcggcgtttt
     1321 tggaactcac gtaccatcat cattaccgat tcctggccac cgcgcagcgc ttccaatgcc
     1381 gcccactggg caatcgaggt ggggttcgag gtactctgac tctgaatctt gccggcggcg
     1441 gcgattgcgt cgctcgcgcc gatggcataa ccgatgcgcc agccggtcat ggcgtaggtt
     1501 ttcgaaacgc cattgatgat gattgtgcgc tcgcgcagat cgccacaaag cgcgacgatg
     1561 ttatgcatct cgaagccgtc gtagataatt ttctcgtaaa tatcgtcggc gaaaatcaga
     1621 cacgaatgtt tttttagaat cgccgcgatt gccagcagct cgtccttgct ataagtcgcc
     1681 cctgtgggat tgcacggact atttaggaaa aacactttgg tctttgctgt tagcccggcc
     1741 tgcagctgtt ctggtctgat gcgaaagccg gtcgactcgt cggtcggaat tagtttgggt
     1801 acgccgcctg ccaacagagc catgtcggcg taactcaccc aatacggcgc gggaatcaac
     1861 acttcgtcgc cgggattgac cgcggcttgg agcaagttgt aaagcgagtg cttggcgccg
     1921 cagctgacca cgacgtcttc cttgcggtag ctgagaccgt attctttttg atatttctgc
     1981 acgatggcct cgcgcagcgg ctcaatgcct ttaacatcgg tgtacttggt catgcctcta
     2041 cgcagcgctt cgatggccgc gtctttgata tgctcagggg tgtcgaaatc cggctcgccg
     2101 gcggaaaaat tgacgatatc gatgccttcc gatcgcagct tgccggcttt ggccgcgatc
     2161 gccaacgtaa ccgagggttt aattaatttg gttcgttcag ccagaaacgt catcgccaga
     2221 tccttgttat ttaaatttct ctttgagttt ttttaggaca ttgtccggca cgagaccggc
     2281 gacgttgccg cctagactga cgacttcttt gaccagccgt gagctggtat agaactccga
     2341 ctcaccggtc atcatgaaaa atgtttcgag ctttggtttg agccggtggt tcatcattgc
     2401 catctgaaat tcatattcaa agtccgaaat cgcccgtagg ccgcggatga ctactgtggc
     2461 acctcggcgc tcggcatagt tgaccagtaa accttgaaag gaatcggcgc tgacacggcc
     2521 atcggcgcga gcgaaaactt cgttgattag ctgcaagcgt tcttccacgg tgaaaagaca
     2581 ccgatcttta tccggattcg ttgctatggc gatgacgact ttgtcaaaaa ctcgtagagt
     2641 gcggttcact aaatcgacat gaccgttggt aattggatcg aaggagcccg gatagatcgc
     2701 gagatcgttg ttagccatta tgattgtcct ctcgcagtcg aattcacgat tttcgtactt
     2761 ggaagaatga taggcaagta tcaccataac ggcgctgatc attcaacacg agatcgccat
     2821 agctcggttt aactgtttcc cgtccgctat gttcgacgac gacgacaccc gcaggactaa
     2881 gcagggaact cttggcgatg tcattgatgg tcgttgcaac caggcctttt tcgtagggag
     2941 ggtcgagaaa gatcaggtcg aatttttcgc cggctttgtc gagcttgcgt aacgagcgcc
     3001 ggactggagc gatccagact tgggcgcgag aagacaaatc cagcttcatt aagttatcgc
     3061 ggatgacggc cgcagtgcga gcggactcgt cgaccaacac cgccgcgaga gcaccacggc
     3121 tgagcgcttc gatgctgacg ttgccgctgc cggcgaataa atcgagcacc cgcagcccgg
     3181 aaaaatcatg ttgcaaaata ttgaatagcg actctttgac ccgtgccgca gtcggccgca
     3241 cggccaggcc cttgggtacc ttgagtttac ggccgcgtgc cgctccgccg attactttca
     3301 ttgccggtca atacgcttcg cagttaacta tttgtgcgca acacaatctt gccgaaatac
     3361 ctgcctttcg ccaaacgctg ttgtgcctgg ccggcatctg ccagatcata aaagctatcg
     3421 ataactggtt tggtgccggt agactcaaaa aagtcgatca gtcgagataa ttcaccgcgg
     3481 gtccctagcg ttgagccgaa gactttcaga tgatgccaaa aaattcgccg caaatccgtc
     3541 aacggttgtg cgccggcgct ggcgccgcag gtcactagcc gtccatctcg gttgagggta
     3601 gcgacactat ttgcccagct tccgccgccg acacagtcaa cgacgacgtc caggccgcgt
     3661 ttgcccgtaa cgcgccgcac ctccgccgcg atatcgtgat cgccttcatt gattccatgt
     3721 tgtgccccta gtcgtaaggc ttgggaaagt ttttcagtag tatcagcggt gacgatggtg
     3781 taggcaccga atcccactgc cagttgcagg gcggccgttg cgacacaatc gccgatacca
     3841 cgaatcaaca ccgattctcc aggtttgagt gctgcgtttg ttagcagcat gcgccaagcg
     3901 gacagatagg cgacgggaat ggcggcggct tcttcatagg cgagtgtcgt gggaatctta
     3961 aaacagtttt gcttgtgaac ctgaagatac tgggcgtacg ttccgtcgac ctgctctcca
     4021 aggcgccgcg gttgctcgca aaggtgttcc tggtcgctgc ggcaagccca gcacgtggtg
     4081 caaccgcttt gcgggtaaaa acaaaccgcg tcgccgatct tcaattcttt gactgcactg
     4141 ccaagcgcca cgatcacacc ggcgccgtcg ctgccgggga tacgcggcaa gcacaatctc
     4201 ttcgagtcgg tgatgccctc acgaacctgg atgtcggcca aattgagcgc ggcagctttc
     4261 aggcggacga tcacatactc gggtgactgg agaatgggat cgttggtctc ttcgtaacgc
     4321 agttgttcga cgccgccgtg atgatgaatg cgtatcgctt tcaagaaaat tcctggacga
     4381 gcatttaatt cgatagcaga aagatcagag tcaaaaacga atctgccagg ccgaagatta
     4441 accgccaagc aaacttattt aggcgtaagg agaattaggc cgaaaaggac gtgccgcaac
     4501 cgcaagtcgc ggtagcgttg ggattgctga acttgaatcc gccgccataa agaccggact
     4561 cgtaatcaat gatcatcccc tggagaaacg gcacgctggc ggcatcgacg aagatcttca
     4621 gcccttgctg gtcgagcacc gtgtcgccgg ctttttgttc tctctcgaag tcgagcttgt
     4681 aagaatgtcc ggagcagccc ccgtcggtga cgctgacgcg caaaccgtga tcggcgcgat
     4741 tgtctcgtgt gaggagtttc ttgatctgct caatggcgcc gttgctgagt ttcagatcca
     4801 ggcaggattg tggattaacc atctccataa ggtggttcta agttaatgcc ggcctacagg
     4861 taagtcaagg tcggttcgtg ttgcgcgggc agcggatctt gtcatcaggg gcggtgaacc
     4921 gcgatttatt tttcaaacag cgcctcgaca aagctttcgg gttcgaacgg ctggagatcc
     4981 tcgacatcct cgccgacacc gataaattgc actggaacac cgagttcctc gtaaatgccg
     5041 ataatcacgc cgcccttggc tgtaccgtcg agtttggtga gcacgatgcc gctaatatcg
     5101 gttgcttctt tgaagacgcg ggcttgctgt aaaccattct gacccgtcgt ggcatcgagc
     5161 accagcagcg tttcatgggg cgcatcgggc aactcgcgag tgatcacccg gcggattttc
     5221 ttgagttcgt cgaccaagtg tactttcgtg tgcaggcgcc cggcggtgtc gatgagcaaa
     5281 acatcgacat tgcggctctt ggccgcttgc atgccgtcga acaccacggc ggagggatcg
     5341 gcgccggctt tatgtttgac aacgtcgaca cccactcgtt tgccccaagc atcaagctgt
     5401 tcgatggcgg cggcgcggaa ggtatctccg gcgaccagta aaactttttt gccgtcggcg
     5461 cggtactgtg cggcgagctt gccgatggtg gtggtcttac cgacgccgtt gacgccaagg
     5521 aaaatgatca cccatgggcg caccgatagc ggcgctcgtt tgggtggggc aactgagccg
     5581 cgcaaaatgt cgatcatggc cgcttgcaac ttcgccatca tatcttgcga caggtcttcg
     5641 atatcgcccg gtgactgacg gcgcaatgcc tgcattaact tgtcggtggc tttgacgccg
     5701 acatcggcgg taaggagact ttcttccatc tcgtcgaggg atttttcgtc ccactggcgg
     5761 ttttgaaaaa tagttccgat cttttgaacc caagtctcac gacttttgct caatccttct
     5821 ttaaggcgtt gaaaaaatgc tccgactgcc atggagtgat actctgcgaa gctaagaaga
     5881 gagtcaaacg gcgggtcgca aatggcggga acgatgcgtg ggaagcttaa ctcatgcgca
     5941 ccgagacgag ttttgataca cccggttcgg ccatggtaat gccatacaac atttcggcag
     6001 actccatggt ctttttgttg tgcgtcacca gcacaaactg cgagtggctg ctcatctcgc
     6061 gaatcatttc attgaaacgg tcgagattgg cgtcatcgag aggcgcatcc acctcgtcga
     6121 gaaagcagaa ggggcttggt ttggttaaga agatggcaaa caacaaactg actgcagtga
     6181 gcgccttttc accccctgaa aggagcgtga tcgattggag ctttttaccc ggcggttgga
     6241 cgatgatatc gacgccggtt tcgagaaagt cattttcatc ggtgagtacg agtttagctt
     6301 taccgccgcg gaacaagcgc gggaatatca tctcgaactt ctcgttgatc gcttcgaagc
     6361 tctctttgaa gcgcagccgg cagatacggt tcaacttgac gattgtttgc tgcaaatcgg
     6421 cgatggactt ctccaaatct tccttttgcc ggcccataaa ctggaaccgg ctagtcagct
     6481 cttcgtactc accgatggca gctagattaa cctcgcccat acgttcaagc cggccgcgca
     6541 gatcttcgat ttcgagcagc agttgatcgc gtgacggtgg tatatcgccg gtttcggcgg
     6601 agagtgagtc caagtcggtg tcgtattttt cgcgcaggtt gtccgccagg tgctgcaagt
     6661 taagacgttt ttccgccagc gttagctgaa tccggctgcg ctcttcctgg caagcttcgc
     6721 caagcgggcg caactccttg atggtttcgc cgatctcggc cagctgcata gaaatagtgc
     6781 ggtagctttg ccggtcggcc tgtaggcgac tctccaacgc ctggagttcc tccttggcgc
     6841 cgcaaagtga cacttcgact tcccgcaacg cctcggcgat ctcactgcgc tggcgttggc
     6901 aatccgtacc gcgcgcctgc cagctggcga tctcttgttc agtctccgct cgctgtttca
     6961 ggcgattgtt aagattggca tgagtgttct cacgcttttc acctaaagcg gcattgcgta
     7021 ttcgtgattg tgtcaccgcg gattcggcgc tttcaacgga accacgtaat tcaccaaagc
     7081 gggcttgttt ttccgccaga gactgttcgc cggccaatct atcggccgtg cgcacttgga
     7141 tcatgtcgta attgcgctgc gactcgtcgc gtagactctg taatgcggcc gcgagatcct
     7201 gttgttcctg gcccagcgct tccagagtgc ggcttaagcg ctcgtattcg tcacccgcgg
     7261 ctcgggtttc gtgctccaac cgcacccgcg cgagttcgag gcgatggatc tcgttggtta
     7321 aaatagtttt gcgggtttct gcctgctcga gttcggctct gagtttctcg aattcagtct
     7381 cctcgtgggg gagttttgca gccaggttcg cgagcgtatc gccgagctca cgaatacgcc
     7441 gcctttgagt gagcagattg ccttcgaggg gggttgtgct gccgcccgta acagtgccca
     7501 tcggatcgat cacttcgcca tcgggggtta cgatcgtgca gtaataacca ttgcggttcc
     7561 aaagcgccaa gcccgcagcc aggtttggca caacaataac gtcagagagc aaatattcgg
     7621 cgatttcgcg atagccgtct ttgatcgata ccaaacccaa tagtggcgcg atgacttcag
     7681 cttcgtttaa gggcaaggcc ttttcctgac gatgacgcag ctgcagcgga ataaagctgc
     7741 cgcgacccga tgcttcttgt ttaaggaatt cgatggattc gacgccttcc tcatggccct
     7801 taacaatgac gtattgaaga cggtcaccca aaacggctgt caatgctttt tcgtagctct
     7861 ccggcgcctc gatcacttca gcgacgagcc cataaatacc gttgggcgag gcggcctgct
     7921 gctttttaag cataatggcg cgcacaccct catgataccc ctcgtaattg cgttggaggt
     7981 cctccagaga gagcaatcgg gaccgattct cttgaatttc cgatttgagg gcctcgatgc
     8041 gattttgttg ttcctcgcgc actcggctga gagtttggat cgaagcgctc accgaggcag
     8101 attcaagcgc gcgctcgcgc aattcctcga cgcaggtgtt caacgcctgg ccagtctcct
     8161 ggcgccgctc ggtgcaagtg gtcagcgata atgtcgcctg cgctgattcg ttttcgcagc
     8221 gcgacagctc ggcggcgatt tccccctgac gtttgtcttt cgccaagttg tcatttttta
     8281 gatatgcgat ttgattcgcg ccatcgatca gcgcggtttt ctcgcggtcg atgttgtcct
     8341 gtagagcgtg gatttgcgcc tgcagaccgc tcaattcggt ttcacgctcg cgcaaaaaag
     8401 attcttcaaa cagcgacaac tgaataaaac tttctttggc tttagttagt tcgtcgatct
     8461 cctgaagaag agtcttcagt cgctcggtta tcgtcgctaa agcaatcttg gcttcgtttt
     8521 ccgcttgttc gagttgttcg agatcgcgcc ggtagaaatc gcatttctgt tcgtcagttt
     8581 ggatctgtgt gcgttgttgg aaaactattt cttgttgcgc cccgatagcc ttgtcggctt
     8641 cgagcagcga taacctcacg gactcgtttt cggcttcctt gctatgaagc atcgccagat
     8701 gttcggcgag acggtcttcg accttggcga gttgtgcctc ctgctgagcg atatcgtcat
     8761 gcaaagcgtc gcgctgaaga gtcgtaaagg caaaatcttt ttcgcgtagt tcagatctga
     8821 gggcgcggta gcgttcggcc ttcttcgcct gtaactccat cgtacggatc tgtctttcga
     8881 cctcgcgaac aatatcgctg acacgcaaaa ggttttgttg ggtacgttcg agttttttct
     8941 cggcgacggc tttgcggctt cgatacatgc tggtcccagc cgcttcttca ataaggccgc
     9001 gccgctcttc cggcttggag ttgactaact catcgacttt gccttgttcg acaatggaat
     9061 atgccttgct gcccacgccg ctgcccaaaa acagttcgac tacgtctttc aaacggcagg
     9121 tggttttgtt gatggcgtac tcggattcgc ccgaacggaa tagccggcgg gttaccatga
     9181 tctcggccaa atgactgtat tccgccggac tgcgaccctc ttcgttgtcg aaggtgatcg
     9241 aaacctcagc cataccggtg ggcggtaagg tatcgctacc ggcgaagacg acgtcctcca
     9301 tttggtgacc acgcaaatgg cgcgcgctct gttcccccat cgcccaacgc agggcatcga
     9361 caatgttcga tttgccacag ccatttgggc ccacgaccgc agtcacaccc ggattaaact
     9421 ctatgaaagt tttctcggcg aaagacttaa aaccgaccat ttcaaggcgt ttgaggcgca
     9481 tctttaattc tattttaaaa tactttttgg cgaatgcagg aagtcggaat tgtcgttaat
     9541 atttcacagc ccattcaagc tgtcaagaat aaatcacaag ataagggaat ctgtggataa
     9601 cttaccacta tatgttgaat gaacgcgttc ccgcgccgaa tcccgggcac gccctgtggg
     9661 taagttacat taccggtggt tttgtcgaga tggtcgctag tttagttttg caggagctcg
     9721 gtattccaat acgaggcgtc gacggtgttc agaaagggcg tccattcctt ataacgtgac
     9781 aaactaaacg tctcctgcat gaatggcgtc cattgagggc gcaagggagc acgcgctagg
     9841 agcataccgg cctgatgcgg tgtgcgcccg ccttttcggc gattgcactc atggcacgag
     9901 caaaccacgt tctcccaggt tgacactccg ccttgagaac gtggaatgac atgatcgaga
     9961 ttgagttcct ggcgcggcag ccgccggtgg caatattggc aggttaactt atcccgggcg
    10021 taaatattga agcggctgaa acgcacttgg cgtttgggaa cccggtcgta ggcgactaag
    10081 agaatgactc ggggcacacg aataacgcga ttgaccatgc cgacggtctc gtcatgggcg
    10141 gaaatcgata ggtcgctcca gctgtcgaaa tcaaaggttt ggtactgctc gttcactgct
    10201 ttggcgatac cctggtagag tagcacgaaa gcacgtttca ccgacgtcac atgaatcggc
    10261 agataggacc gattgaggat caggaccttg gtgtgaagga tcgaataacc tggcgcggtg
    10321 gctgaacaac tcataatgag cttgcatcgt gaatgctgat cgagtcgccg gtactcttgc
    10381 gaaagtaccg gtctccggcg cgaatgtcaa tttgaccggg acgagtggtc agcttcaact
    10441 tgatctcacc cgggatttct tttataacgc acctagctac tttctttttt tgctattcgt
    10501 cggttgcgct attctctgcg cggcccgctc cgtactgcac ccaccggcat cgccaaaagt
    10561 ttagtgtcaa cgtaaaatat gaaacaagaa atcattatta actccacacc gcaggaaagc
    10621 cgcatcgcga tcgtcgaaga cggtctactg gccgagttct tcgtcgaacg aaaagaagaa
    10681 atgggtgtcg cggggaatat ttacaagggc aaggtcgctc gggttttgcc tgggatgcaa
    10741 gctgccttca tcgaaattgg catggacaag gcgggatttc tccacgcctc ggatttctcc
    10801 acccccgatg atgttcatgt catcagcccg gccaacgacg atgtcgaatt cgaggaggcg
    10861 ccgccgaagt ccaatccgtc gcgctacttg cccctggaga agcagctttc ccgtggtgaa
    10921 gaagtgctcg tgcaggtagc caaggatcct ctcggcacta aaggcgcccg cgtcacgtcg
    10981 catatttcgc tgcccggacg ctacatggtt tttatgccgg gcaataagca tatcggtatc
    11041 tcgcggcgca tcgaaagtga tgaggaacgc aagcggctca aggaaatcgc ccagtccttg
    11101 gggaccgacg aaggcggctt tattctgcgc acggcgtgtg agggactgag taagcgcgag
    11161 attcaacgcg acttaggttt cctgcagaag ctctggaaaa gcattcagaa gaaagccgaa
    11221 accgcgccgg cgccgtcgtt gatccatcag gatctcgatc tgattgcgcg cgcgattcgc
    11281 gactatttca gcgccgatac cgaacaggtg ctgatcgatt cgcccaagga tcatcggcgc
    11341 atcgtcgatt ttgtccgcca ttttatgccg cgcttgaagt cgaaaattaa tctttactcc
    11401 gacaaggagc cgctgttcga gcagctcggg atcgaagaaa aaataaccaa ggcgctcgtt
    11461 ccgcgcgcct ggttgcgttc gggcggctac atcatcatcg agcggaccga ggcgttaacc
    11521 gcggtggacg tcaacaccgg ccgcttcgtc ggcaagcgta atcaagaaga aacgatcctc
    11581 aagaccaacc tcgaagcggt gcaggaagtg gtgcgtcaac tgcggctgcg aaatgccggc
    11641 gggctcatca tcatcgactt catcgacatg gataaagagg ccaaccgcaa gaaagtctac
    11701 gaagctcttc
//
LOCUS       DATLCH010000234         5188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5108138, whole genome shotgun sequence.
ACCESSION   DATLCH010000234 DATLCH010000000
VERSION     DATLCH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5108138"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..2692)
                     /gene="ptsP"
                     /locus_tag="VMZ02_06895"
     CDS             complement(293..2692)
                     /gene="ptsP"
                     /locus_tag="VMZ02_06895"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HUR71694.1"
                     /translation="MAKNTKIARTTHPHLGILADISTLITHSHDLQETLDRLVSTVAE
                     RMQTEVCSLYIFDAKKNRLTLCATTGLDPEAVGRVSMGTGEGLTGLVIEQMKPVMVVD
                     ALAHKRYKYFPETHEEHFHSFLGVPLFEKKAPLGVLVVQTSRRREFSRDEIRLLTTIS
                     GQAASIIVQARLADNLRSKEEERKEYQKRMNEAMRKLRSYENVPPEKTSKAKQKWRGR
                     LTGLAASPGFGRGQAFVLEPRMELNAIRKKKSRNPRHEIERFRGAVERGIDQINVVKR
                     RMSNLISAEEGAIFDVYRLIFEDPQIIHQVEQQIRKGHYVAEYAVRVVFERYMDSIAK
                     LDNSYLRERTADVKDAAQRLLETLSGGEGQQYDIPDDAILVAQDLSPADLSMLEGDKF
                     KGIVLATGGVTSHASILAKSFEIPSIVAVEDLTERVRHGDLLIVDGNSGGVHVNPSAE
                     VIREYDRLEKDYADLNRELGGLKDLPAQTTDGHRVSICANVGLLSDVAFAHLHGAQGI
                     GLYRTEIPFLSHRDFPSEEEQYALYKRVVEGMMNKPVTIRTLDIGADKYPTYMRSVAS
                     EPNPFLGWRSIRISLEVEEIFKTQLRAILRVGDLGMVRMLVPMISSLEEIQKVKDLLA
                     EARAELEREGTPYDRQMELGIMVEVPSAVQLAERFLREVDFLSIGTNDLIQYLLAVDR
                     SNRKVANLYEPLHPAVLMALKATIDAGKKVGKRVSMCGEMAGDPLCALVLLGMGLEEF
                     SMGALYLPVIKKAIRSISYQAAKTTAQMVLAMDTVGEIKRYLFEQIRELEMVELLEMY
                     R"
     gene            2836..3246
                     /locus_tag="VMZ02_06900"
     CDS             2836..3246
                     /locus_tag="VMZ02_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71695.1"
                     /translation="MSQIAGLRGMRHIALKVRDIARAKAFYQDVLGMKVVWEPDDKNV
                     YLSSGCDNLALHEAAEISPMSARQGQLDHLGFIVDSIERVRELEQEFLGQSIPIVHPF
                     KIHRDGSASFYCADPDGIIIQMLYEPQLSVQTIT"
     gene            3670..>5188
                     /locus_tag="VMZ02_06905"
     CDS             3670..>5188
                     /locus_tag="VMZ02_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71696.1"
                     /translation="MYDLLIKGGRLYDGSGLPSYHGDVAIADGKIVDTGRVSGSAKRT
                     LSVDGLAVAPGFIDPHTHLDAQLFWDPMGTSSCFHGVTSVVVGNCGLSLAPAKPEDRE
                     AVIKSFVRVEAISRRVLEEGIQWRWRSTAEYLDVLGAHLGINVAALVGHIAVRHYVMA
                     EEAVERGATPDEIAKMKQLVRQGMEAGAVGFSTNQNPRHVREDKKPVASRLSTDEELG
                     SLLDVLGEMNAGVVQLSGGGTDARGRIDYATGLARRTGRPVLWQSISHSWTRPDHWRG
                     MLDSTAKVFHEEGLPIYAMTQAKPFEMRYSLLDAQCFDEFPTWKTAMFSPVETRKQMF
                     ADSDCRKKLRAEAIDDKSASVFPRRWDVVNIDKVKLAKNKSFERQSVEEVARRQGKDG
                     LDCFLDLSLEENLETRFSHITTQGDPKAVCEILKHPAVVIGQSDAGAHMGYDARFGYC
                     TAFLGRWVRDYGIMTLEEAVSKLTFRVASLFGLSDRGLVRPGFAADLAIFDPATIN"
BASE COUNT         1108 a         1394 c         1486 g         1200 t
ORIGIN      
        1 cttcggctga cctttgaaat caccgaactc gatctccggg gaatgaagtt cgccgagttt
       61 ggtttcgaag ttgtaatcac ccagcagtgc cgcatcgtag ccgatgtctg tctgccactg
      121 atggaaatag tcgatccagt catcgaaagt tgagattttg atcatgggag gtccctttca
      181 atttagccgt aagttcgaac gatcgttcga attgtttata gcggtttgcc ggggctaaat
      241 caatgcccct taaacttgaa tgggctgaag ccgcgagcag ggggtcttcg gcctagcgat
      301 acatttctag cagttcgacc atttccaatt cgcgaatttg ttcaaaaaga tagcgtttga
      361 tctcaccgac ggtatccatc gcgagaacca tctgtgccgt cgttttggcc gcctgataac
      421 tgattgagcg aatggctttt ttaatcaccg gcagatacag agcccccatg ctgaattctt
      481 ccaagcccat gcccaaaagt accagcgcgc acaacggatc gccggccatc tcgccgcaca
      541 tgctcacgcg tttgcccact ttcttcccgg catcgatggt ggcttttaat gccatgagaa
      601 cggccggatg gagcggttcg tatagattag cgactttgcg gttactgcgg tcgacggcga
      661 ggagatactg aatcaagtca ttggtgccga tactcaggaa atccacttcg cgcagaaacc
      721 gttccgcaag ttgcaccgcc gacggcacct cgaccataat gccgagctcc atctgccgat
      781 cgtagggcgt gccttcgcgt tctagttcgg ccctggcctc agcgagtaga tctttgactt
      841 tctgaatctc ttcgaggctc gaaatcatgg gcacgagcat gcgcaccatg ccaagatcgc
      901 cgacgcgcaa aatggcgcgc aattgtgtct tgaaaatctc ttcaacttcg agcgagatac
      961 ggatcgaacg ccagcccaag aacggattgg gctcgctagc gacgctgcgc atgtaggtcg
     1021 gatacttatc ggcgcctatg tcgagggtgc gaatggtgac cggcttattc atcattccct
     1081 cgacgacgcg cttataaagc gcgtattgct cttcttcgct ggggaaatcg cggtgtgata
     1141 aaaacggaat ctcggtccgg tacaggccaa ttccctgggc gccgtggagg tgcgcaaagg
     1201 cgacgtcgct tagcaggccg acgttggcgc agatcgacac tcggtgccca tcggttgttt
     1261 gcgccgggag atctttcaat ccgccgagct cacgattgag atcggcatag tctttttcca
     1321 accgatcata ctcgcgaatc acttcggcag acggattgac atggacgccg ccggagttgc
     1381 cgtcgacgat tagcagatcg ccatggcgga ccctttcggt caaatcctca accgcgacaa
     1441 tgctgggaat ttcgaacgac ttggccaata tcgacgcatg ggaggtaacg ccgccggttg
     1501 ctaaaacgat acctttgaac ttatcgcctt cgagcatact caaatcggcg ggagagagat
     1561 cctgcgccac cagaatcgca tcgtcaggga tatcgtactg ttgaccctct ccgccggata
     1621 gggtttccaa tagccgctgt gcggcatctt taacgtcggc cgttcgttcg cgcaggtagc
     1681 tgttgtcgag cttggcgatg gaatccatgt agcgttcgaa gaccacgcgg acggcgtatt
     1741 ccgcgacata gtggcctttg cgaatctgct gttcgacctg gtgaatgatc tgcgggtctt
     1801 cgaaaattag ccgatagacg tcgaaaatcg caccttcttc ggccgaaatc agattgctca
     1861 tgcgccgctt aacgacgttg atctgatcga tgccgcgctc cactgctccg cgaaagcgct
     1921 cgatctcgtg ccgcggatta cgcgactttt tttttctgat cgcgttaagt tccatgcgtg
     1981 gctcaaggac aaacgcttgg ccgcggccga agccaggaga tgccgctaac ccagtcaagc
     2041 gaccgcgcca tttctgcttg gcttttgagg ttttttccgg cggcacgttt tcataggagc
     2101 gcagcttgcg catcgcctcg ttcatgcgct tctgatattc cttacgctcc tcttccttgg
     2161 aacgcaggtt gtcggcgagc cgagcctgga cgattatact ggccgcctgt ccggaaatgg
     2221 ttgtcagaag acgaatttca tcccgagaaa attcgcgacg ccgagatgtc tgcacaacca
     2281 agacgccgag cggcgccttc ttttcgaaca gcggtacgcc aagaaacgag tggaaatgct
     2341 cttcgtgggt ttccgggaaa tacttgtaac gtttgtgcgc cagggcgtcg acgaccatta
     2401 ctggcttcat ttgctcgatg actagaccgg tcagtccctc gccggtgccc atggatactc
     2461 tgcccaccgc ctcgggatcg aggcccgtgg ttgcgcacag ggttagacgg ttctttttgg
     2521 cgtcgaaaat gtatagcgag cacacttcgg tctgcatgcg ttcggcgacg gtggacacca
     2581 gccgatccaa agtctcttgc agatcgtgcg aatgagtaat cagcgtgctg atgtccgcga
     2641 gtatccccag gtgagggtgc gtggtgcggg caatcttggt attttttgcc atggcagctg
     2701 attgatgatg aagttcgagt tctctataaa acacaaattt cgcgaattgg ccaggtagcc
     2761 aagcggtcac agctcggtag ttcgccgtgg attaagcgca actgcattat cctatcgatg
     2821 tgatatattg ttgttatgag ccagatcgct ggtttgcgag gcatgcgtca tatcgcgcta
     2881 aaggtgcgcg acatcgcgcg cgcgaaagcg ttttaccagg acgtactcgg catgaaggtg
     2941 gtctgggagc cggatgataa gaatgtttat ctatcatcgg gctgtgacaa cctggcgctc
     3001 catgaagccg ccgaaatttc accgatgtcg gctcgtcaag ggcagctcga tcatctcgga
     3061 ttcatcgtcg atagcatcga gcgggtgcgt gagttggagc aggaatttct cggtcaaagc
     3121 attcccatcg tccatccttt caagattcat cgcgacggca gcgcgtcatt ttattgcgct
     3181 gatcccgatg gcatcatcat tcaaatgctg tacgagccgc aattgagcgt acaaaccatc
     3241 acgtagctcg gccatcggcc ttagctacgt ttgcctgatg aaagctttcc tgccgcacta
     3301 tgcgtcgcga ggtttgctgc agcgattagg taaactccag gccgacaaag gcatggcgtg
     3361 taccgacgca catggatcat gcagacggac cgcatgttta agccaagcca ggatcgaagc
     3421 cttgcggcag cagccactcg atgattccat tcattcacgg agcagcatcc cgacagcaga
     3481 gggcatctga aggtcgcatg gctctcgtct ggacagtagc agcgcattta gttactcccg
     3541 cctgcgattc cgtactacca taacggcctt gaccgctcgt aaagacatcg actaaggttc
     3601 actgcaaatg ctggctggta gtggcgggcg tgaactgcac taatttgcca actcggaaag
     3661 gaaagcacga tgtacgatct tttgatcaaa ggcggacggc tttacgacgg ttcggggctg
     3721 ccgagctatc acggtgatgt tgccatcgcc gacgggaaga ttgtcgatac cgggcgagtc
     3781 agcggcagtg ccaaacgcac gttgagcgtc gacggtctgg ccgtggctcc gggattcatc
     3841 gatccgcaca cgcatctgga tgcgcagctt ttctgggacc caatgggaac atcgtcctgt
     3901 tttcacgggg tgacttcggt ggtagttggc aactgcggtt tgagcctggc gccggctaaa
     3961 cccgaggacc gcgaggcggt gataaaaagt tttgtccgcg tcgaagcgat cagccgccgc
     4021 gtgcttgagg aaggcatcca gtggcgctgg aggtcgacgg cggaatactt ggatgtcctg
     4081 ggcgcgcacc tgggtatcaa tgttgccgca ttggttggac atatcgctgt gcgccattac
     4141 gtcatggccg aagaggcagt ggagcgggga gcgacgccgg atgaaatcgc aaaaatgaaa
     4201 cagctcgtgc gccaaggcat ggaagccggc gccgtcggat tttccaccaa tcagaacccg
     4261 cgtcacgttc gcgaagacaa gaagccggtc gccagccgtc tttccaccga tgaagaatta
     4321 gggtctttgc tcgacgtcct gggcgaaatg aacgcgggtg tcgtgcagtt gagcggcggc
     4381 ggtaccgacg cgcgcggacg cattgattac gccactggat tggctcggcg taccggccgg
     4441 ccggtgctct ggcaaagtat cagtcatagc tggacgcggc ccgatcattg gcgcggcatg
     4501 ctggactcga cggcaaaagt tttccacgaa gagggtttgc cgatctacgc catgacccag
     4561 gccaaaccgt tcgagatgcg ctacagcttg ctcgacgccc aatgcttcga tgagtttcct
     4621 acctggaaga cggcgatgtt cagcccggtg gaaacgcgca agcagatgtt cgccgacagc
     4681 gactgtcgca agaagctgcg ggccgaagcg atcgacgaca aatcggcgtc ggtgtttccg
     4741 cggcgctggg atgtggtcaa tatcgataag gtgaaactcg cgaaaaataa atcctttgaa
     4801 cggcagtccg ttgaagaagt tgccaggcgc caaggcaaag acggtctgga ttgttttctc
     4861 gatctgtcgc tggaggaaaa tctcgaaacc cgtttctcgc atatcaccac ccagggcgat
     4921 cccaaggcag tgtgcgagat tctcaaacat ccggcggtgg tgatcggcca gtccgatgcc
     4981 ggcgctcaca tgggctatga tgccaggttc ggttactgta cggcattctt gggccgctgg
     5041 gtgcgcgatt acggcatcat gacgttggaa gaagcggttt cgaagttgac cttccgtgtt
     5101 gcctcgctgt ttgggcttag cgatcgcggt ttagtccggc ccggctttgc cgctgatctg
     5161 gcgatcttcg atccggcaac catcaaca
//
LOCUS       DATLCH010000235         3393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7766507, whole genome shotgun sequence.
ACCESSION   DATLCH010000235 DATLCH010000000
VERSION     DATLCH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7766507"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VMZ02_06910"
     CDS             complement(<1..191)
                     /locus_tag="VMZ02_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71697.1"
                     /translation="MRQLKVVARMVNALGLVCEKIIVFVLVWILAAYATAAETSVNSD
                     ALPMFGQPKIARSDSQKQA"
     gene            291..1061
                     /locus_tag="VMZ02_06915"
     CDS             291..1061
                     /locus_tag="VMZ02_06915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HUR71698.1"
                     /translation="MIQGLKDKVVIVTGGGHGIGRAYCHGFAEAGAKVVVADIDAPAA
                     EKVAGEVVKQFDGKALAAKVDVANEQSTKDMAKLTLDNFSRIDVLVNNAAIFSTIPMN
                     RGGIDTIDPAEWDRMMTVNLKGLFFCTRAVLPAMRSQKSGKIINISSGTALNGSAGRI
                     HYVTSKAGVIGFTRTLAREVGEDNINVNAIAPGSTLSEENPSEEVLKMRGARLADRAL
                     KRVQLPKDLVGTMLFLASPLSDFMTGQTVAVDGGVCFL"
     gene            1071..1892
                     /locus_tag="VMZ02_06920"
     CDS             1071..1892
                     /locus_tag="VMZ02_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71699.1"
                     /translation="MAAYQHRTIYVGDIRTHFLEAGTGPDLILLHGGEYGASAEITWR
                     HNIDLLAKTFHVIAPDTLGWGQTEKLYSFSDPAGLRIRHLQRLLEALGIGKAFFVGNS
                     AGGGLILRASVRQPPPLQISKMVTICGNASVFKTNSQADLENYTPSLENMKKIVALLY
                     HDKKWQSDENIRERYESSILPGAWEALSAARLRSPVHQVRSTTDEFTKQLSQLTTPLL
                     LMSCEHDPLNQSDWDVNFQRIVPGAKVHRFKHSAHEPQIEETDEFNRVLTEFLLG"
     gene            1903..2910
                     /locus_tag="VMZ02_06925"
     CDS             1903..2910
                     /locus_tag="VMZ02_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71700.1"
                     /translation="MKIMPICRVTALGLICHCIVLLASASAQAQLTRLRTSYSALTAN
                     MGAYWLAKDTKLFEKHGLDVDVVLIENGTITIQALIAGETDLAMGGGTVAVSSALGGS
                     DLVSISSIESRLPYGLLAQKEIKSIDQLKGKRHAISRFGSVADLGSRLILQKFGLVPD
                     KDVTLLQIGGTSTRLAALSKRTVDSTILTPEFFLVAKKAGFTILVDPTQYKIDFPQLE
                     VITTRTFLKSRPDVATRYMRAIVEGIHNYKNDREGSIRALSKYLRINDREALDEVYRI
                     YYDIYQPVPHPSPAAIQTQLSWMAEKDPRAKAAKPEQFIDGSIMREIEKSGFAAKLYQ
                     K"
     gene            complement(2939..>3393)
                     /locus_tag="VMZ02_06930"
     CDS             complement(2939..>3393)
                     /locus_tag="VMZ02_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71701.1"
                     /translation="KQIDGQVMPVPWDYRSRKLGLNILARAEDLFTYPISGLITQTRK
                     IKERPDEIRRVIKAGIKANRYIHANRDGTIEVLMSTYRLDKEAATALYASLVKGFNDD
                     GNLPEDGLRRLIDDVKSVTKVDREIAFSEVSDISILREAQRDLGIRTK"
BASE COUNT          813 a          974 c          868 g          738 t
ORIGIN      
        1 tcggcttgct tctggctgtc cgatcgggca attttcggtt ggccaaacat tggcaatgcg
       61 tccgagttca ccgacgtttc cgccgccgtc gcatatgccg ctaggatcca taccaggaca
      121 aacacgataa tcttttcgca aaccaaaccg agcgcattta ccatccgagc gaccaccttt
      181 aattgacgca tagtctctga attgatgatt agacgcgcca agtcaccttg acacctaaaa
      241 acatctaagg ctattttgac ggcaaatcaa attaatggag taaccttttt atgattcaag
      301 gactcaaaga caaagttgtc atcgtcacag ggggcggcca tggcatcggc agagcctatt
      361 gtcacggttt tgccgaagcc ggagcgaaag tcgtcgttgc cgatatcgac gctcctgccg
      421 ccgaaaaagt cgccggcgaa gtagtcaaac agtttgacgg caaagccttg gcagctaagg
      481 ttgacgtcgc caacgaacag tcgaccaaag atatggccaa attaaccctc gacaatttca
      541 gccgcatcga tgtgctggtc aacaacgcgg cgattttctc gactattccg atgaaccgcg
      601 gcggcatcga caccatcgat ccggcggaat gggatcgcat gatgacggtt aatctcaaag
      661 gtttattttt ctgcacccgc gccgtactac cggcgatgcg cagccagaag agcggtaaga
      721 tcatcaacat ctcgtcgggc acggcgctca atggcagcgc cggacgcatc cactatgtca
      781 cctcgaaagc aggcgtcatt ggctttaccc gcacgctggc gcgtgaagtc ggcgaagaca
      841 acatcaacgt caacgccatc gctcccggat cgaccctgtc ggaggagaat cccagcgaag
      901 aagttctcaa gatgcgtggc gcgcgcttgg ccgaccgcgc gttgaagcgc gtacagttgc
      961 ccaaggatct ggtcggcacc atgttattcc tcgcttcacc cttgagcgac ttcatgaccg
     1021 gacagaccgt tgccgtcgac ggtggcgtgt gtttcctata actgaattcc atggccgctt
     1081 atcaacaccg gacgatctac gtcggcgaca ttagaacaca ctttctcgaa gccggcaccg
     1141 gccctgatct gatattgctt cacggcggag aatacggcgc ctcggcagaa atcacctggc
     1201 gccacaacat tgacctattg gcaaagacgt ttcacgtcat cgctcccgac acgttgggtt
     1261 gggggcaaac cgagaagctt tacagcttca gcgatcctgc cggcctgcgc atccgacatt
     1321 tgcaaaggct tctggaagcg ttgggcatag gtaaagcctt tttcgttggc aattccgccg
     1381 gcggcggctt gatactgcgc gcatcggtgc gccaaccgcc acccctgcag atcagtaaga
     1441 tggtcaccat ctgcggcaac gccagtgtat tcaagaccaa ctcccaggcc gatttggaaa
     1501 actacacgcc ctccctggaa aacatgaaaa agatcgtcgc cctgctctat cacgataaaa
     1561 aatggcagag cgatgagaat atccgcgagc gttatgaaag ttcgattttg ccgggcgctt
     1621 gggaagcgct ctcggcggcg cgcctgcgca gcccggtgca tcaggtgcgc tcgaccactg
     1681 atgaattcac caaacaattg tcccagctga ccaccccgct gctgctcatg tcctgtgagc
     1741 atgacccgct caaccagagc gactgggatg tcaattttca aagaattgtt cccggcgcca
     1801 aagtgcatcg cttcaagcat tcggcccacg agccacaaat cgaagagacc gatgagttca
     1861 atcgggtgct gacggaattc ttattagggt gaggtgcctg tcatgaaaat catgccgatc
     1921 tgccgagtca cagcactcgg gctgatctgt cattgcattg tgctgctggc ttcggcgagc
     1981 gcccaagctc aactcacccg actgaggacc tcttatagcg cactgacggc caacatgggc
     2041 gcttactggc tcgccaagga taccaagctg ttcgaaaagc atggattgga tgtagatgtc
     2101 gtgctgatcg agaacggcac gattaccatt caagcgttga tcgccggcga aaccgatctc
     2161 gccatgggcg gcggcactgt tgctgtgagt agcgcgctcg gcggtagcga tctggtatcc
     2221 atttcgagca tcgaaagccg cctgccctac ggcttgctcg ctcaaaaaga aatcaaatcc
     2281 atcgaccagt tgaagggcaa acggcacgca atttcacgct tcggcagtgt cgccgatctc
     2341 ggttcgcgct tgatcctgca aaagttcggc ttggttcccg ataaggacgt cacgctactg
     2401 caaatcggcg gcaccagcac gcggctggcg gcgctgagca aacgaacggt cgactcgacg
     2461 attctaacgc cggaattttt tctggtcgcc aaaaaggccg gattcacaat actcgtcgat
     2521 ccgacacagt acaaaatcga ctttccccag ctcgaagtca taaccacaag aacgtttctc
     2581 aaaagccggc ctgacgtagc gacacgttat atgcgcgcca tcgtcgaagg tattcacaac
     2641 tacaaaaacg accgcgaagg cagtatccgc gccttgagca aatatctgcg catcaatgat
     2701 cgcgaagcct tggacgaggt ctatcgaatt tactacgaca tctatcagcc cgtgccgcat
     2761 ccatcaccgg cggcaattca aacccagctg tcgtggatgg cagaaaaaga tccgcgcgcc
     2821 aaagcggcca aaccggaaca attcatcgac ggctcgataa tgcgtgagat cgagaaaagc
     2881 ggcttcgcgg cgaagcttta tcaaaagtag atccgcccgg gcattttacg ggccggtgtt
     2941 acttcgtcct aatgccaaga tctctctgcg cctcccgtag aattgaaata tcggaaactt
     3001 cgctgaatgc gatttcccga tcgactttgg tcaccgactt gacgtcgtcg ataagcctgc
     3061 gcaacccgtc ttccggcaga ttgccgtcgt cgttaaagcc tttcacaagc gaagcgtaca
     3121 gtgccgtggc ggcctcctta tcgagccggt acgtgctcat taacacttcg atggttccgt
     3181 cacgattggc atgaatgtag cgattagcct tgatcccggc cttgatcact cggcgaatct
     3241 catccggccg ttcctttatt tttcgcgtct gagtgatcag tccgctgatc ggataggtaa
     3301 ataaatcttc ggctcgcgct aaaatgttca aaccgagctt gcggctacga taatcccacg
     3361 gaaccggcat tacctggcca tcgatctgtt tct
//
LOCUS       DATLCH010000236         6775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_644011, whole genome shotgun sequence.
ACCESSION   DATLCH010000236 DATLCH010000000
VERSION     DATLCH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644011"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VMZ02_06935"
     CDS             <1..468
                     /locus_tag="VMZ02_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HUR71702.1"
                     /translation="NVIAVMNAGVIVQEGAPRDIYLQPKTQFVANFIGSTNQMDGQVS
                     KLEGDGTATVRTDDGEISCGILDGLTAGSKVVVVVRPESLNLHLQKPANLANVIEGKI
                     GAAMFLGEYVDCTIELGKNILQTHQRYTLQVRRGDPVWLELPAAECMALPANR"
     gene            587..1585
                     /locus_tag="VMZ02_06940"
     CDS             587..1585
                     /locus_tag="VMZ02_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71703.1"
                     /translation="MLNKLLTLIASTALFFAAVNTNLGAQEKIKVVYSSADATNFVWY
                     AAQDGGFYKKHGLEVELIFVPSSTTAVSSMIAGDIQIGNNSGGTVASAVVSGANLVLT
                     GCYINTLPYELVVHESIKSADELRGKSIGISRVGSASDSAARVLIKGLGLEPVKDVPI
                     LQVGGTAERAAAFRGGRIVGFPSPPGVIHLAQGMPHRILISTADFQKRYEFPFICSST
                     TKTFLAARRDTVRRVTMALIEATQFLKTRKEESKRFIGKFARQNNPRYLEDSYNAMAK
                     LHDRIPVVGRVGAEEQIKESLSRKPGATMKAEDWIDDSIVRELDKSGFIDKVYGQK"
     gene            1749..2339
                     /locus_tag="VMZ02_06945"
     CDS             1749..2339
                     /locus_tag="VMZ02_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HUR71704.1"
                     /translation="MIVAVVLSAGESSRMGRPKALLPIEGETFIERIVSALKQGGIER
                     TLVVLGFNAEDLQRKIAHLPVEILLNPNYKLGQLSSLQVAIRHLDADQNCEGMMVHLV
                     DHPYIDSKLVSLMLQRFESSKSPIVVPRHHGKRGHPVIFSRALFGELLGAPFDQGAKA
                     VVNAHRQETLEIDTDDAGITLDIDTPELYRQHVKGE"
     gene            2340..3311
                     /locus_tag="VMZ02_06950"
     CDS             2340..3311
                     /locus_tag="VMZ02_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71705.1"
                     /translation="MAKLQLTLACGDYDFLRPLINGEIQPQGIELNVLTMASPERHGR
                     MLKHEEFDVCELSLVGYLVSRDKGCGYNAIPVFPHRRFRHQYMVKRTGCGIEKPADLN
                     GKRIALDTLQNSAGLWMRGILQDHYDVDLKTIEWWCQEEEDIPFEPAAWMNVKRVGHG
                     KNIDQMLVNGELEASLYPETLPAIRSGNPKVALLFPDPKTAEIEYYKNGGHFPIMHTV
                     VVKNPILEKHPWVGISLVQAFERAKQICYARNSDPRSFALVWVQDLIREQKEIFGADP
                     WPYNLEDNRKALEAVIRYEFEQGMIKKNPRVEELFFPPSLERIQHYV"
     gene            complement(3573..5180)
                     /locus_tag="VMZ02_06955"
     CDS             complement(3573..5180)
                     /locus_tag="VMZ02_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUR71706.1"
                     /translation="MDKLPNFLFIITDQHRADHLSCYGNRIVKTPAIDRLAQAGTRFD
                     NFYVATPICMPNRATLMTGRMPSLHGARQNGIPLSLTATTFVDILRAAGYDTALIGKC
                     HLQSISGARPTIGMPEADPDRVQPPEHLREADKSWLSHGRYDQELRSTWQNDPSFELA
                     LPYYGFNQVQLAVGHGDEVVGHYDRWLNQRKPDADKLRGRKNQLPGNCYVAPQAWRTA
                     IPEELYPSAFVAEQTIKYLENYSRSDRSQPFFLQCSFPDPHHPFTPPGRYWDMYNPQE
                     IPLPQSFNPGERPIVPHLRRLYQERAAEQANKDGQRTFAVTEREVREATALTYGMISM
                     IDDAVASILGWLKTLRLDNDTVVIFTSDHGDFMGDHQLLLKGALHYRGLVRVPFIWSD
                     PANESTGDLNSGLCGTIDLAPTILHRAGLAAPNGMQGKSLIEAMKGAATGHDSMLIEE
                     HQRRGYMGLANNFRARSLITKQHRLTLYEGVGWGELYDFSSDPHELDNLWNAPSAQPL
                     RQDLTEQLARKMMEMTDTSPLATHHGP"
     gene            5296..5811
                     /locus_tag="VMZ02_06960"
     CDS             5296..5811
                     /locus_tag="VMZ02_06960"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HUR71707.1"
                     /translation="MIDSAVLIPVYRSQIGELHIVMILRKPGGVHGGQIAFPGGKHDP
                     EDDTMLDTALREAREELGLIVPREDILAELPMEQTRTTGYRVFPYVARIALPEQWQIA
                     EREVAAIVDVRLSELVAPGAHDRMIERFPTWQKAERVSFYRIGEYRLWGLSYRILQPI
                     IPRLVAGDWNV"
     gene            5935..>6775
                     /locus_tag="VMZ02_06965"
     CDS             5935..>6775
                     /locus_tag="VMZ02_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR71708.1"
                     /translation="MDFILQQDSPEISAFRKEVREWLAQNMKGSEHLRWSGNWSTREN
                     DEEYKFRRALAGKLGQKRWLFPTYPVKYGGAGLSLDHQFVVETEIDRYGLSLGSVFYT
                     LARLVAPAVMKFGTEEQKMEFLPPMARGELAVWQVLTEPQGGSDVANCQTKAIRDGDH
                     YVVNGQKVLVGHHLPPDYLWTFVCTNPAGKRHENLGWLYIPANLPGITLQHMHLMMGI
                     KNAVFFDNVRVPAKYMVGGENNGWKVANSYLELEHGGGGSVAEQPLFKRIIEHCQKTD
                     FDGE"
BASE COUNT         1496 a         1771 c         2001 g         1507 t
ORIGIN      
        1 aacgtcatcg ccgtaatgaa tgctggtgtg attgttcagg agggcgcacc gcgggatatc
       61 tatttgcaac cgaaaaccca atttgtcgcc aactttatcg gttcgaccaa tcagatggat
      121 ggccaggtga gcaaactgga aggagacggc accgcgacgg ttcgcactga cgacggcgag
      181 atatcttgcg gtatcctcga cggcttgact gcgggcagca aggtggttgt ggtggtgcgg
      241 cctgagagcc tcaatcttca tctgcaaaag ccagccaatt tggcaaatgt tattgaaggc
      301 aaaatcggcg ctgcgatgtt tttgggtgaa tacgtcgact gcaccatcga gcttggcaag
      361 aacattttgc agactcatca gcgctacaca ttgcaagtcc gccgaggcga tccggtctgg
      421 cttgaactgc ccgccgccga gtgcatggcc ctgccggcca atcgctaatc gatgcttcgg
      481 caatctcctc atcattgttc cgctgtcaaa tcgttctatc gagacgttga atcccaatac
      541 taaaattcta tgcgccggcg atcggctcag gaagattaat ctatcgatgc ttaacaaatt
      601 gctgactcta atcgcgtcga cggcattgtt tttcgctgct gtgaatacga atttgggcgc
      661 tcaggaaaag atcaaggtgg tgtacagctc ggcggatgcg acgaatttcg tctggtatgc
      721 cgcccaggat ggcggctttt ataaaaagca tggtttggaa gtggagctga tcttcgtgcc
      781 gagttcgacc acggcagtct ccagcatgat cgccggcgat attcagatcg gcaataattc
      841 cgggggcacg gtagcgagcg cggtggtcag tggcgccaat ctggtgctga cgggttgtta
      901 catcaacacg ctaccgtatg agttagtggt ccatgaatcg atcaaatcgg ccgatgagtt
      961 gcgcggcaag agcatcggca tcagccgcgt tggcagcgcc tcggattcgg cggcgcgggt
     1021 attgatcaag gggcttggtc tcgagccggt gaaggacgtg ccgatcctcc aggttggcgg
     1081 cacggccgag cgcgccgcag cgtttcgcgg cggacgcatc gtcggttttc cttcgcctcc
     1141 aggtgttatc catttggcgc aaggcatgcc gcatcggatc cttatcagca cggcggactt
     1201 tcagaagcgc tacgagttcc cgttcatctg ctcgtcgacc accaaaacct ttcttgccgc
     1261 tcgccgcgac accgtgcgcc gggtgaccat ggcgctgatc gaagcgacac aatttctgaa
     1321 gacccgcaag gaagagagca aacggtttat cggtaagttc gcgcgccaga ataatccaag
     1381 atatttggaa gattcctaca atgccatggc caagctccat gaccgcatac ccgtggtcgg
     1441 gcgcgtcggc gcagaggagc aaattaaaga atcgctaagt cgcaagcccg gcgccacgat
     1501 gaaggccgag gactggatcg atgacagtat cgtgcgcgag ctcgacaaga gcggtttcat
     1561 cgacaaagtc tacggtcaga aataatgcta agcagcttcg agttgctctt tatcgctaag
     1621 aaaatccccc tcaatccccc tttttcaaaa ggggacggtg ttggacgtgg agtttggttt
     1681 ttccccctct ttggaaaaga ggggttaggg gagattttcg tttgcggtaa tcagctccaa
     1741 atcaccccat gatcgtcgcc gtagtgctat ccgccggtga atcgagccgc atgggccgcc
     1801 ctaaggcgtt gttgccgatc gagggcgaga cttttatcga acggattgtc agcgcactta
     1861 aacaaggcgg tatcgaacgc acccttgtgg tcttgggatt caatgccgag gacttgcagc
     1921 gtaagatcgc tcatctgcct gtcgaaattc tgctcaatcc caactacaaa ctcggacaac
     1981 tgtcatcgct gcaggtggca attcgtcacc tagatgcgga tcagaattgc gaaggcatga
     2041 tggttcacct ggtcgatcat ccatacatcg attcaaaact ggtcagtctc atgctgcagc
     2101 gctttgaatc atcgaagagc ccgatcgttg tgccgcgcca ccacggcaaa cggggccatc
     2161 cggtgatatt ttcgcgcgca ctgttcggtg aattgctcgg cgcgccgttc gaccagggcg
     2221 ccaaagctgt ggtcaacgcc catcgccaag agacactgga gatcgacacc gacgacgcgg
     2281 gcataactct cgatatcgac acgccggaac tctaccggca acatgtgaaa ggcgagtaga
     2341 tggcaaaact gcaactgaca ttggcttgcg gcgactacga tttcttacgc ccgctcatca
     2401 atggcgaaat acaacctcag ggtatcgagc tcaacgtttt gacgatggcg tcgccggagc
     2461 gccacggccg catgctgaag cacgaagagt tcgacgtttg cgagctgtcg ctcgtgggtt
     2521 atctagtctc acgcgacaaa ggctgtggtt acaatgccat tccagttttc ccgcatcgcc
     2581 gttttcgtca tcagtacatg gtcaagcgca ctggctgcgg tatcgagaag ccggccgatc
     2641 tgaatggcaa gcgaatcgcg ctcgatacct tgcagaactc tgccggtctg tggatgcgcg
     2701 gcatcctcca ggaccattac gatgtcgact taaagaccat cgaatggtgg tgccaagagg
     2761 aagaagatat cccattcgaa ccggctgctt ggatgaacgt gaagcgggtg gggcacggca
     2821 aaaatatcga tcagatgttg gtcaacggcg aattggaagc ctcgctgtat cccgaaaccc
     2881 tgccggcaat acgctcgggc aatcccaaag tggcattgct gttccccgat cccaagacag
     2941 ccgagatcga atactacaaa aatggcggcc attttccgat catgcatacc gtcgtggtca
     3001 agaaccctat actggaaaaa catccgtggg tgggtatcag tctggtgcag gccttcgagc
     3061 gcgccaaaca aatctgttac gcgcgcaaca gcgacccgcg cagcttcgcg ttggtctggg
     3121 tgcaggatct gattcgcgag cagaaagaaa tattcggtgc cgatccctgg ccttacaacc
     3181 ttgaggataa ccgcaaagct ttggaagcgg tgattcggta cgaattcgag cagggcatga
     3241 tcaaaaaaaa tccccgcgtc gaagaactgt ttttcccgcc gagtttggag cgcatacagc
     3301 attacgtcta agcgctccgc gcgattatta cgaagttcca gacgttccaa tcgttcaagt
     3361 cggtcaaaac gtttctgcag cttcgggaag caggcacgcg cgactgaatg cctctagctg
     3421 caggccagcg gactacaggt cgcgcgcgaa agtcgagttt tcctgtttgt cattccggcg
     3481 taaggccgga atgcagcttt tgaccaggac taaacgccgg cttcgtgaga tacttcacaa
     3541 gatatgctcc aactcgaagc gactcccacg catcaggggc cgtgatgcgt tgccagagga
     3601 ctcgtatcgg tcatttccat cattttgcgc gcgagttgtt cggtcaaatc ctgccttagc
     3661 ggttgggcgc tgggcgcatt ccagagattg tcgagttcat gaggatcgct ggagaaatcg
     3721 taaagctctc cccagccgac tccttcgtac aacgtcaagc ggtgttgttt ggtgatcaga
     3781 ctgcgggcgc gaaagttgtt cgcgaggccc atatagccgc gccgctggtg ttcttcgatg
     3841 agcatcgagt cgtgtccggt tgcagcgcct ttcatcgctt cgattaaact tttgccttgc
     3901 atgccgttgg gtgcggccag accggcgcga tgtaaaatcg tcggcgcaag atcgattgtg
     3961 ccgcacagcc cggaattgag atcgccggtg ctttcattgg ctggatcgct ccagatgaac
     4021 ggcacacgaa ccaggccgcg ataatgcagc gcgcctttga gcaacaactg gtgatcgccc
     4081 atgaagtcac cgtggtcgct ggtgaatatc accaccgtgt cgttgtcgag ccgcagcgtt
     4141 ttaagccagc ccagaatgct cgcgacggcg tcgtcaatca ttgagatcat gccgtaggta
     4201 agcgcagtgg cttcgcggac ttcccgttcg gtgaccgcaa aggtgcgctg accgtccttg
     4261 ttggcctgct cagcggcgcg ttcttggtac agccggcgca ggtgcggcac gatgggccgc
     4321 tcgccgggat taaacgattg tggcaaagga atctcttgcg ggttgtacat atcccaatag
     4381 cgccccggcg gcgtaaacgg atggtgcggg tcgggaaacg aacactgcag gaagaacggc
     4441 tgggagcggt cgctgcgcga ataattttcc aagtacttga tggtctgctc ggcgacgaaa
     4501 gcgctgggat agagttcttc cggaatcgcc gtgcgccagg cttgcggggc gacatagcaa
     4561 ttacccggca gttggttctt gcggccgcgc agtttgtcgg catcaggctt acgctgattg
     4621 agccagcgat cgtagtgtcc gaccacttcg tcaccatggc cgacggcgag ctgaacctgg
     4681 ttaaaaccgt aatacgggag cgcgagttcg aaggaaggat cgttctgcca ggtcgagcgc
     4741 agttcttgat cgtagcggcc atggctgagc caactcttgt cggcttcgcg taaatgctcc
     4801 ggcggttgca ctctgtcggg atcggcttca ggcatgccaa tggtcgggcg agcgccactg
     4861 atgctttgca ggtgacattt gccgatcagc gcggtgtcgt agccagccgc gcgcaagata
     4921 tcgacgaagg tggttgcggt gagtgaaagc ggaatgccat tttgccgcgc gccgtgaagg
     4981 gacggcatgc gcccggtcat caacgttgcc cggttgggca tgcaaatcgg cgtggcgacg
     5041 taaaaattgt cgaagcgggt gccggcttgc gctaaacggt caatggccgg agtcttgacg
     5101 atgcgattgc cgtaacaact caaatgatcg gcgcggtgct gatcggtgat gatgaataaa
     5161 aaatttggca gcttgtccat tttgtatcgc ctgtctaata tcacaactca tcgactgtgc
     5221 aattagtaaa atcgcgccgt tctttgccac aagcaggcca agtgtagtag aagcggagtt
     5281 aaggtaaatt ttttgatgat cgactccgcg gtgctgattc cggtctatcg ctctcaaatt
     5341 ggcgaactgc atatcgtcat gattttgcgt aagcccggcg gcgtgcatgg cgggcagatc
     5401 gcctttcccg gcggcaagca cgatccggaa gacgacacca tgctcgatac tgcgctgcgc
     5461 gaggcgcgcg aagagctcgg cttgatcgtg ccgcgtgagg atatcttggc tgagctgccg
     5521 atggagcaga cacgcaccac cggctatcgg gtttttccgt acgtcgcccg catcgccctg
     5581 ccggagcagt ggcagatcgc cgagcgcgag gtcgccgcca tcgttgacgt acggctgagc
     5641 gaattggtcg cgccgggagc gcacgataga atgatcgagc gcttcccgac ctggcagaaa
     5701 gccgagcggg tgtcgtttta ccgcatcggc gaatatcggc tatggggatt gtcgtatcgc
     5761 attctgcagc cgatcattcc gcgtttggtg gcaggcgatt ggaatgttta atcgggatgt
     5821 gatttgtgat cgctgaattc caacgaggat ctcgcggcac gaatagggat tatgtgcata
     5881 tcgcgttgtc gtgtatttat ctgattgcag gaagtttgta ggaggaaaca agacatggat
     5941 tttattttac agcaggatag cccggagatc agcgcatttc gtaaagaggt gcgcgagtgg
     6001 ttggcgcaaa acatgaaagg cagcgagcat ctgcgctggt cgggcaactg gtcaacccgc
     6061 gaaaacgatg aagagtataa gttccgccgt gctctggccg gtaaactggg ccagaaacgc
     6121 tggttatttc caacctatcc ggtgaaatat ggcggcgctg gtttaagctt ggatcatcag
     6181 ttcgtcgtcg aaacagaaat cgaccggtat ggtctctcgt tggggtcggt attctacacg
     6241 ctggcgcggt tggtggcgcc ggccgtgatg aagttcggca ctgaagagca aaagatggag
     6301 ttcttgccgc cgatggcgcg cggcgaattg gcggtctggc aagtcttgac cgagccgcaa
     6361 ggcggcagcg atgtcgccaa ctgccagacc aaagcgattc gcgatggtga tcactatgtc
     6421 gtcaatggcc agaaggtgtt ggtcggacat catttgccgc cggactatct ctggacgttt
     6481 gtttgcacca accctgccgg caaacgccac gagaatctcg gctggcttta catcccggca
     6541 aacttgccgg gcatcacact gcagcacatg catttgatga tgggcatcaa gaacgcggtg
     6601 ttcttcgaca atgtccgggt gcctgcgaaa tatatggttg gcggcgagaa caatggctgg
     6661 aaagtggcaa acagctatct ggaactcgaa catggcggcg gcggcagcgt ggctgagcag
     6721 ccgctgttca agcgcatcat tgagcattgc cagaagaccg acttcgacgg cgaga
//
LOCUS       DATLCH010000237         4140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10760081, whole genome shotgun sequence.
ACCESSION   DATLCH010000237 DATLCH010000000
VERSION     DATLCH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10760081"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VMZ02_06970"
     CDS             complement(<1..1301)
                     /locus_tag="VMZ02_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HUR71709.1"
                     /translation="MQTELKRQIGLFSAVMLIAGDMIGTGIFISTGAIAETLPTPGGV
                     LLVWLFGGLLALAGALTCAELAASLPYAGGDYIYIREAYGKLMGFLSGWSSFLVTFSG
                     AIAFLSVILNGFMAFFFPVLGSEDVLFSLALPVIPITVKIGTLFSMGTVLVLSGLHCL
                     GVRQGTVTQNILTILKIGALLGIILLGIFFGDGNSSHFEPLFDWEKIGNSSLFGAAFI
                     PVIFAYSGWNAVTYIAGEVKDPEQNLPRALMYGVSIVIALYLAINAVYIYAVPVTEMK
                     GALRMSEVATTALFGYKTSAWITAIITVSILGALNVVTMIGPRIYYAMAKDGVFFHQL
                     TYVHPNFGTPTKAILLQALWSCLLILTNTWGTLFTYVSVVITLFSAFTVGSVIVLYLA
                     IIAVYIYAVPVTEMKGALRMSEVATTALFGLKTSAWITAIIT"
     gene            1554..2219
                     /locus_tag="VMZ02_06975"
     CDS             1554..2219
                     /locus_tag="VMZ02_06975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR71710.1"
                     /translation="MKLFVTLVGLLLAVTAQAQDAAKRDQHQMHRLHTDPAAYINALD
                     DPKRDEYQKPHQVLTALNIKPGEVIADIGAGTGYFTFRLAHFVGDKGKVYAVDVSPEM
                     IRHINRRTRDSKTTNVISILADNDDPLLPERSVNRIFVCDVWHHVENQTKYLAALKKI
                     LRPGGEVVMIDFHKKELPFGPPLQMKIAREDLVKQMESSSFRLSKEYTFLQYQYFLVF
                     TPK"
     gene            complement(2230..3327)
                     /locus_tag="VMZ02_06980"
     CDS             complement(2230..3327)
                     /locus_tag="VMZ02_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71711.1"
                     /translation="MVTESRRIIDADGHVAENSAAIIARMPQAYRDKAREQPFNPFPP
                     FDHLHAGHLVDMPPGAFNRKAGPKEWLAFLDEVGIEATVLYPTAGVASANIVNPDWAI
                     DATRAYNEWLHETYLQASPRFRGLALLPQALINSQAAGDELRRAVTELGMCGAVLPSN
                     SLHAPPLGTPTYFPVYEAANELGCCVAVHGGVHGNMGMDGLDPYAAVHAIGHPLGQMV
                     SLASLIFNGVMDRFPKVRFGFLEGGVAWFLFCLERFDRSYDTHVMRDLRGSYLKLQSG
                     EKVSDYIIRHVKDRRIFIGCEGGEPLIGKAVQMVGNEPFMYSSDFPHEVNAETCKKEL
                     HELIDNRELSVADKDVILYKNAIRFYGIAGD"
     gene            3725..>4140
                     /locus_tag="VMZ02_06985"
     CDS             3725..>4140
                     /locus_tag="VMZ02_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71712.1"
                     /translation="MKIMVVGSSGYIGRHVVKNLAAQGAEVIAFDLVPAATQWSVPGV
                     PFIQGNMTNLEEVMSAVLENGVQRIIALGYYMTPLLSPECRDLLNAARVNIIGVTNLF
                     EAARLAKIPRVVFASTVGIFGYQDGYGPEPIDENTEP"
BASE COUNT         1039 a         1201 c         1060 g          840 t
ORIGIN      
        1 gtaatgatcg cggtaatcca agccgaggtc ttgagcccga acagcgccgt ggtcgcgact
       61 tccgacatgc gcagcgctcc cttcatctca gtgaccggaa ccgcataaat ataaacggcg
      121 ataatcgcca aatagagcac gatcaccgag cccaccgtga aagcggaaaa cagcgtgatc
      181 acgacactga cgtaggtgaa caacgtgccc caagtgttgg tgagaatcag caagcaactc
      241 catagcgctt gcagcagaat tgccttggtt ggagtgccga aattcggatg gacgtaggtg
      301 agctggtgga agaacacccc gtccttggcc atcgcgtaat atatccgcgg ccctatcatg
      361 gtcaccacgt ttagggcgcc gagaatggaa acggtgataa tcgccgtgat ccacgccgaa
      421 gttttgtagc cgaaaagcgc cgtggtcgcg acttccgaca tgcgcagtgc gcctttcatt
      481 tcggtcacag gcaccgcgta gatataaacc gcgttgattg ccaagtagag cgcaatgacg
      541 atcgacacgc cgtacatcag cgcccgcggc aagttctgtt cggggtcctt aacttcgccg
      601 gcgatatagg tcaccgcatt ccaccctgag taggcgaaga ttaccggtat gaacgcggcg
      661 ccgaacaaac tggagttgcc gattttttcc cagtcgaaaa gcggctcgaa atggctcgag
      721 ttgccgtcgc cgaaaaaaat gcccaggaga atgattccca agagcgcgcc gattttcaaa
      781 atcgttaaaa tattttgcgt taccgttccc tgtctaacac cgagacagtg tagtccggac
      841 aaaaccaaca cggtgcccat cgagaacagc gtgccgattt ttacagtgat cggtatcacc
      901 ggcagcgcta acgaaaagag cacgtcttcc gagccgagca cgggaaagaa aaatgccatg
      961 aagccgttca atatcaccga aagaaatgcg atggcgccgg aaaaggtgac taggaacgaa
     1021 gaccagccgg agagaaaccc catcagcttg ccgtaagcct cacggatata aatgtagtcg
     1081 cctccggcgt agggcaaact ggccgccaat tcagcacagg tgagcgcgcc ggcaagcgcg
     1141 agcaacccgc cgaagagcca aacaagcagt accccgcccg gtgtcggcaa ggtctcggca
     1201 atggcgccgg tggagataaa aattcctgtc ccgatcatgt ccccggcaat cagcatcacc
     1261 gctgaaaata aaccaatttg ccgtttaagc tcagtttgca tagcgactag gaatacaggg
     1321 aaacgagaaa acaggcaaat cttttccgtc gcaagagtgg ctagttcgca agatcccact
     1381 cctacttcgg aatgacggta atttatcatg taagccgagc gagaaatcta tgcgaacgag
     1441 ccacagaacc ttttgaaagg gccacgacgg tccgccacgc cgctcttaat cacttaccgt
     1501 ggaaattcta gtgctaattc cggcgcactg tcggcgattt tgaaggagaa gttatgaagc
     1561 tgttcgtgac gttggtcggc ttgttgctcg cggttaccgc ccaagcccag gacgcggcga
     1621 agcgcgatca gcaccaaatg caccgattgc atactgatcc cgccgcttac ataaatgccc
     1681 tggacgatcc caagcgcgac gaatatcaaa aacctcatca agtcttaacc gccctgaata
     1741 taaaacccgg cgaagtaatc gcggacatcg gcgccggcac aggctatttc acatttcgcc
     1801 tggcccattt tgtcggagac aagggcaaag tctacgcggt cgacgtcagc cccgagatga
     1861 tccgccacat caaccggcgc actcgcgatt cgaagaccac caatgtcatt tccatcctcg
     1921 ccgacaatga cgatccgctg ctgccggagc gctcggtcaa tcgcattttc gtctgtgatg
     1981 tctggcatca tgtcgagaat cagacaaaat atctcgcagc gctgaaaaag atactcagac
     2041 caggcggcga ggtcgtcatg atcgatttcc acaagaaaga attgcctttc ggccctccgc
     2101 tccaaatgaa aatcgcccgc gaggatctcg tcaagcaaat ggaatcgagt agcttccggc
     2161 taagcaagga atatacgttt ttacagtatc aatacttcct ggtgttcacg cccaaataat
     2221 cgtcacgggt taatctcccg caatgccata gaagcggatt gcgtttttgt agaggatcac
     2281 gtctttatcc gctactgaca gttctcgatt gtcgatcagc tcgtgcaact ctttcttgca
     2341 agtttcggca ttcacctcat ggggaaagtc gctggaatac ataaacggct cgttgccgac
     2401 catctgcacg gctttgccga ttagcggctc gccgccttcg cagccgatga agatacgcct
     2461 gtctttaaca tggcgtataa tataatcgct gactttctcg ccggattgca gtttgagata
     2521 actgccgcgc agatcgcgca tgacatgagt gtcgtaggag cggtcgaacc tttccaagca
     2581 gaatagaaac cacgccactc cgccctcgag aaaaccgaag cgaactttcg ggaatctatc
     2641 cattacacca ttgaaaatca gcgacgccag cgaaaccatt tgtcccagcg gatggccgat
     2701 ggcgtgcacc gccgcatatg ggtcgagacc atccataccc atgttgccgt gcacaccgcc
     2761 gtgaacggca acacagcaac cgagttcgtt ggccgcttca tacactggaa aataagtcgg
     2821 cgtgcccagc ggcggcgcat gcaaactgtt cgacggcagc accgcgccgc acattccgag
     2881 ctcggttacg gcacgccgca attcatcgcc cgcagcctga gaattgataa gtgcttgcgg
     2941 caagagcgcc aagccgcgaa agcgtggact cgcctgtaaa taggtctcgt gcagccattc
     3001 gttgtaggcc cgcgtggcat cgatggccca gtccgggttg acgatattcg ccgacgccac
     3061 gccagcggtc ggatagagca cggtcgcttc gatgccgact tcgtccaaaa acgccaacca
     3121 ttccttcggc cctgccttgc gattgaatgc acctggcggc atatcgacga gatggccggc
     3181 gtgcaaatga tcgaaaggcg ggaacggatt gaacggctgc tcgcgcgcct tgtcccgata
     3241 ggcctgcggc atccgcgcga tgatcgccgc cgaattctcg gcgacatgac cgtcggcgtc
     3301 gatgatccgt ctggattctg tgaccatatt taccaattcc ttccctatac gattggctca
     3361 cactccactc accaaacctt cccagccttt cttacatcct tcccctcgac ggggaaggac
     3421 cgaggaaggg gtgcccaggt ttttcaaaaa cgtgcgcagc atccccatcc taccccatca
     3481 gaggctgtgt cgtgttgcgc aaatgcctaa cctgtcatcc cgagcgcagc gagggatctc
     3541 atgttttcag ccaaatacga agaaaagatt tctcggcttc accgcgaaat gacagttgcg
     3601 acacagtctc tcaagacgac tcactgaata ccaaagccat tgagcaatac gcaacgcatt
     3661 aaacattgcg ttattcgacc acagcatcgt atagatcaac cagtcttatt tggaggcagt
     3721 gcccatgaag atcatggtcg tcggcagcag cggctacatc ggccgccacg tcgtaaagaa
     3781 tttagctgcg caaggcgcgg aagtcatcgc cttcgatctc gtgcccgcag ccacgcaatg
     3841 gtcggtgccc ggagtgccat tcattcaagg caacatgact aacctcgaag aggtgatgtc
     3901 ggcagtcctg gaaaatggcg tccaacgcat catcgccctc ggttactata tgacgccgct
     3961 gctctcgcct gaatgccgcg acctgctgaa cgccgcccgc gtcaacatca tcggcgtgac
     4021 caacctattc gaagccgcgc gcttggcaaa aattccgcgc gtcgtcttcg ccagcacagt
     4081 cggcatcttc ggctaccaag acggctacgg tccggagccg atcgatgaaa acaccgaacc
//
LOCUS       DATLCH010000238         6989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4348325, whole genome shotgun sequence.
ACCESSION   DATLCH010000238 DATLCH010000000
VERSION     DATLCH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4348325"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1166)
                     /locus_tag="VMZ02_06990"
     CDS             complement(72..1166)
                     /locus_tag="VMZ02_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUR71713.1"
                     /translation="MMPDLRKRGSSPYWKKPKQGFSCVPAIWIVLVLSGQGSVQNVHG
                     ASEILAEVDGLAITSDAIEKPLAAQLSKLEEQIYELKRQKLDALINEKLLANEAAKRN
                     LTVPVLLDAEVTSKVGLITEQEIEKFYEDNKAQIKSEQAQVHEQIRAFLQNQKLATKR
                     EEFLKSLSARAKVLVHLKAPPIQRIAVSVQGAPFKGGTNAPVTIVEFSDFHCPFCQRV
                     LSTLAQLKSRYGENIKLVFRDFPIESLHPGAIKAHEAARCANEQGQFWAYHDKLFASP
                     PSSNPDVLKELAKEVGLDTSTFATCLSSGKYQAAIKEDMAEGSRIGVDGTPAFFINGR
                     QIIGAQPLDGFVRVIDDELAREGSIQSEAR"
     gene            1324..2262
                     /locus_tag="VMZ02_06995"
     CDS             1324..2262
                     /locus_tag="VMZ02_06995"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HUR71714.1"
                     /translation="MILVAVKDATVESLIQRYAGHLCNERNMSPHTSRNYLSDLAQFQ
                     KFLIERELALNKHGRVDAGKVDIHIVRAYLASLAADRKKSSIGRKLAVLKGFFRYLVT
                     TKQLAKDPLLLINSPKQEKPLPKFLTVDDVFHLLEAGKLENGLDLRDQAVLEVFYSTG
                     IRVSELVGLDWADIDRQLGIIRVVGKGSKERIVPIGLVALKALESYAETVRLRWAVVC
                     RGETPVFLNHRGRRITTRSVARIVEKHLKLAGIEVKMGPHGLRHSFATHLLNGGADLR
                     VIQELLGHVSLSTTQRYTHLDLDQLTAIYDKAHPRA"
     gene            2370..2900
                     /gene="hslV"
                     /locus_tag="VMZ02_07000"
     CDS             2370..2900
                     /gene="hslV"
                     /locus_tag="VMZ02_07000"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HUR71715.1"
                     /translation="MFEGTTILAIRHGGKVVVVGDGQVSLGQTVMKRGARKVRKLHHD
                     KVIAGFAGATADAFTLFEKFEAKLEQFNGNLKRAAVELAKDWRTDRILRRLEALLIIA
                     DVTDLLVISGAGDVIEPDDGVIAIGSGGNYALAAARVLVKHTQLDARVIAEEAMRVAA
                     GICVYTNEQLTFEELP"
     gene            3045..4424
                     /gene="hslU"
                     /locus_tag="VMZ02_07005"
     CDS             3045..4424
                     /gene="hslU"
                     /locus_tag="VMZ02_07005"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HUR71716.1"
                     /translation="MNDVSKLSDDAGTAPAPVMTPREIVSELDRYIVGQKDAKRAVAI
                     ALRNRWRRQLVPEELRDEIAPKNIIMIGPTGVGKTEISRRLAKLAQAPFIKVEASKFT
                     EVGYVGRDVESIIRDLTDLAINMVKEEEKQKVEIRAREVAEERVLDLLLPAGTPVDGE
                     AIDAARLQGTRDKLKKMLRDGKMDDRFVEIEISQSVMPMIEVLTPQGMEGMEFNLKDM
                     FSNMMPKKTKKKTAKIPEALEILTQEEAAKLVDMDKVTGEATRRVEQSGIVFLDEIDK
                     IAGRESVHGPDVSRQGVQRDLLPIVEGCTVNTKYGMVRTDHILFVASGAFHTAKPSDL
                     IPEFQGRFPIRVELSSLGKSDFVRILTEPKNALIKQYEALLETEGVHLKFADDAIQEI
                     AAIAADVNERMENIGARRLHTILERLLDEVSFAAPEMHGQEIAIDAAYVRERLTPILQ
                     SEDLSRYIL"
     gene            4439..5323
                     /gene="argB"
                     /locus_tag="VMZ02_07010"
     CDS             4439..5323
                     /gene="argB"
                     /locus_tag="VMZ02_07010"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HUR71717.1"
                     /translation="MDDFIGKAEVLMEALPYFQRFYGKTFVIKYGGHAMENEELKASF
                     AQDIVLLKYVGINPVVVHGGGPQIDAVLVKMGVKSHYVRGMRVTDQETLDIVEMVLVG
                     KVNKEIVNLINQHGGMAVGLSGKDGGLILAKKMNVTVASPNGESPEIIDIGMVGEIIG
                     INPLVIESLDQNKFIPVIAPVGVGKKGETYNINADLVAGEIAEALHAEKFLLMTDVEG
                     VKDKKGALMSTLTEKQARKMIQDGVVGSGMIPKVECCINALKGGVEKTHIIDGRVKHA
                     VLLEIFTKQGIGTEVVRK"
     gene            5324..6511
                     /locus_tag="VMZ02_07015"
     CDS             5324..6511
                     /locus_tag="VMZ02_07015"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HUR71718.1"
                     /translation="MKNRDVAALTDKFIAGTYARTPLALVKGRGVKVWDADGKEYLDF
                     LAGIAVNSFGHCHPAIVKAIKQQAQVLMHVSNLYHMQPHAELARELCRNSFADRVFFC
                     NSGAEANEAAFKLARRYGQEKLGGKYEIISTHNSFHGRTLATLTATGQEKVRAGYDPL
                     PLGFRQVPFNKLEAMEAAIDEKKTVAILIEPIQAEGGVNVPHETYMLGLREICDQRRI
                     LLIFDEVQTGMGRTGKLFGYENFGVKPDIMTLAKALGGGLPLGAMLAREDVASSFNPG
                     SHASTFGGNPVSCAAGVAVMQLLKSGVLKNCVQMGKYFVKGLEALKKRFPFIREIRGK
                     GLLIGVELEIDGSKIVESCMLEGLLMNCTAAKVLRFVPPLTIKKNEIDRGLASLEKVL
                     ARQ"
     gene            6528..>6989
                     /locus_tag="VMZ02_07020"
     CDS             6528..>6989
                     /locus_tag="VMZ02_07020"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HUR71719.1"
                     /translation="MKKRDLLSFDGMTRREFDGLLQLAGELKRKQKRGIAHRLLAGKG
                     MALVFEKPSLRTRSTFAVGMTQLGGSSVYLGPSEVGLGTRESPADCARNLDRWFDLVT
                     VRTFAHKIIEEMAKYSSVPVINALTDTYHPCQVLADCQTLIEHKGRLDGLKI"
BASE COUNT         1627 a         1818 c         2022 g         1522 t
ORIGIN      
        1 cgatttcaat atgaccgaaa gaatgtttca gagaagtgca agaggcacgc ctcaactacg
       61 gattctaatc tctagcgggc ctcgctttgt atcgagccct cgcgcgctaa ttcgtcatcg
      121 atgacacgca cgaacccgtc caatggttga gcgccgataa tctgccggcc attaataaaa
      181 aacgccggcg tgccgtcaac gccgatgcgg ctgccctcgg ccatatcttc cttgatcgcg
      241 gcttggtatt ttccgctgct caggcaggtt gcaaaagtgc tagtgtcgag gcccacttcc
      301 ttggccaatt cctttaagac gtcgggattc gaactaggcg gactggcaaa cagcttgtca
      361 tgataggccc aaaattgtcc ttgctcgttg gcgcagcgcg ccgcttcgtg agcttttata
      421 gctcccggat gcaggctctc gatcgggaaa tcacgaaaga caagctttat gttttcaccg
      481 taacgcgact taagctgcgc gagcgtagaa aggacccgtt gacaaaacgg acagtgaaag
      541 tcggagaact ctacaattgt cacaggggcg ttcgtgccgc ccttgaaggg cgccccttgc
      601 accgacactg cgatacgttg gatcggcggc gccttgaggt ggaccaagac cttggctctt
      661 gcgctgagtg atttaaggaa ctcttcacgc ttggttgcca gcttttgatt ttgtaaaaaa
      721 gctctgatct gttcgtggac ttgtgcctgc tcgctcttta tttgcgcctt attatcctcg
      781 tagaactttt cgatctcctg ctcggtgatc agcccgacct tgctcgtgac ctcggcgtct
      841 agcaacacgg gcaccgtgag gtttcgctta gctgcttcat ttgccagcag cttttcattg
      901 atcagtgcgt caagtttctg gcgttttaac tcgtagatct gctcttcgag tttgctcaat
      961 tgcgcagcaa gtggcttttc gattgcgtcg gaagtgatgg caaggccatc aacttccgcc
     1021 aatatctcac tggcgccgtg gacattctgc accgacccct gtccagaaag cacgagcaca
     1081 atccaaatag ccggcacgca agaaaatccc tgcttcggct ttttccaata cggcgaggaa
     1141 ccgcgcttgc gcaaatcagg catcatagtt cggctgaatc ttggctgaca gaacaactaa
     1201 taatctagga tctgttgtag cgcagcggct cacggcaaaa aagagcgaag gtgttttcat
     1261 ctttttctgg gaaccatgtg cgagtttagt atacaatcgc tgattttcgt tcacagagca
     1321 ggcatgatat tggtagcagt gaaagatgcg actgtggaat cgctaattca acgatacgcc
     1381 ggtcaccttt gcaatgagcg caacatgtcg ccgcacacgt cgcgaaacta tctaagcgac
     1441 ttggcgcagt ttcaaaaatt cctgatcgaa cgggagctgg cgttgaataa gcacggtagg
     1501 gtggacgcag gcaaagtcga cattcatatc gtgcgagcgt atttagcctc cttggcggcg
     1561 gatcgtaaga aaagttcgat cggccgcaag ctggcggtgt taaaaggctt ctttcgctat
     1621 ttggtgacga ctaaacaact cgcgaaggat ccgttgttgt tgatcaactc gccgaaacag
     1681 gaaaagccct tgcccaagtt tctcaccgtc gatgatgtct ttcatttgct cgaagcgggt
     1741 aaacttgaga acggtctcga tctgcgcgac caggcggtgt tggaagtatt ttattccacg
     1801 gggattcgcg ttagcgaact ggttggactt gattgggcag atatcgacag gcagctggga
     1861 atcattcgcg ttgtcggcaa aggttctaaa gagcgcatcg tgccgatcgg cctggtggct
     1921 ttgaaagctc tagaatccta tgccgaaacg gtacggctcc ggtgggcggt ggtctgccgt
     1981 ggcgaaacgc cggttttcct taaccaccgt ggtcggcgta ttacgacgcg cagcgtcgcg
     2041 cgcattgtcg agaaacacct caaacttgcc ggcattgagg tcaaaatggg ccctcatggc
     2101 ctgcgtcata gcttcgctac tcatttactg aacggcggcg cagacctgag agtgatccaa
     2161 gagttgctcg gccatgtcag cctatcgacg acgcagcgct atacccattt agacttggat
     2221 caactgacgg caatttacga caaggcgcac ccgcgagcgt gaaggaaaag gcatgaggct
     2281 cgaggcgcga ggcatgagta agatgaagac cgctccgagt actctgactc aagcctcgcg
     2341 gctcatgcct cgcgccaagg aattttcaaa tgtttgaagg aacgacaatt ctagcgatcc
     2401 gccatggcgg taaagtagtc gtcgtcggcg atggtcaggt aagtttgggc cagaccgtaa
     2461 tgaaacgcgg cgcgcgcaag gtgcgcaagt tgcatcacga caaggtgatc gccggtttcg
     2521 cgggcgcgac tgcagatgcg tttacgctgt tcgaaaagtt tgaagccaag ctcgaacagt
     2581 ttaacggaaa cctcaagcgt gcagcggtgg agctggccaa agactggcga acggatcgga
     2641 ttctgcggcg gctggaggca ttgctgatca tcgccgacgt gaccgatctg ctggtgatct
     2701 ctggtgccgg cgatgtcatc gaacccgatg acggcgtgat cgccatcgga tcgggtggca
     2761 actatgcgct ggcggcggcg cgcgtgctag tcaagcacac tcagctcgac gcacgggtga
     2821 ttgctgaaga agcaatgcgg gttgccgccg gcatctgcgt ttacaccaac gagcagctga
     2881 ccttcgaaga actgccctag tcgctcggcg cgcacggcga ctcgcgttca aactacgatt
     2941 ccaattcatg ccgggatgac ctgttactga ggcaccattc accttggctt ctggcaggta
     3001 aatcattgca tttcctggcg gaattgttag gagtgtttat tcccatgaac gacgtttcaa
     3061 aattgtctga cgatgccgga acggcaccgg cgccggtgat gacgccgcgc gagattgttt
     3121 ccgagcttga ccgctatatt gtcgggcaaa aagacgctaa acgcgccgtc gccattgcct
     3181 tgcgcaaccg ctggcggcgc caattggtgc cggaggaatt gcgcgacgag atcgcgccga
     3241 agaacatcat catgatcggc ccaaccgggg ttggcaagac cgaaatatcc cgccgcttgg
     3301 cgaagctcgc gcaggctcca ttcatcaaag tagaagcctc taaatttact gaggtcggct
     3361 atgtcggacg cgatgtcgaa tcgatcatcc gcgacttgac cgatctggcc atcaacatgg
     3421 tcaaagagga ggagaagcag aaagtcgaga ttcgggcacg cgaagtggct gaggagcgcg
     3481 tactggattt gctgttaccc gccggcacgc cggtcgacgg cgaagcgatc gacgccgccc
     3541 gtctgcaagg cactcgagat aagctcaaga agatgctgcg cgacggcaag atggacgacc
     3601 gcttcgtcga gatcgaaatt tcgcagtcgg tgatgccaat gatcgaggtg ctgacgccgc
     3661 aggggatgga aggcatggag ttcaatctca aagacatgtt ctccaacatg atgccgaaaa
     3721 aaaccaagaa aaagacggcc aagatacctg aggcgctaga gattctgacc caggaggaag
     3781 cggcgaagct ggtcgacatg gataaggtta ccggggaggc aacccggcgc gtcgagcagt
     3841 ccggtatcgt ttttctcgac gagatcgaca agatcgccgg gcgcgagtcg gtgcacggac
     3901 cggatgtctc ccgtcagggc gtgcagcgcg acctgttgcc catagtcgaa ggctgtaccg
     3961 tcaacaccaa atacggcatg gtccgtaccg atcacatttt gtttgtcgct tccggcgcct
     4021 ttcacacggc taagccgtcg gacttgattc ctgagttcca ggggcgtttc ccaattcgcg
     4081 tggagctcag ttctctcggt aagtccgact ttgtgcgcat cctcaccgaa ccgaaaaacg
     4141 cgctgataaa acagtatgaa gcgctgctcg aaaccgaagg cgtgcatctc aagttcgccg
     4201 acgatgcgat acaagagatc gccgccattg ctgccgacgt caacgaacgt atggaaaata
     4261 ttggcgcgcg ccggctacat acgattcttg aaagacttct tgacgaagtc tcgtttgccg
     4321 cgccggaaat gcacggtcag gagatcgcta ttgacgccgc ctacgtgcgc gaacggctga
     4381 caccgatctt gcagagcgaa gatttgtcgc gctacatcct ctaaattacg gacccgagat
     4441 ggacgacttt atcggcaagg ccgaggtgtt gatggaggcg ctgccatatt ttcagcgctt
     4501 ttacggcaag accttcgtca taaaatatgg cggccacgcc atggagaatg aggagctcaa
     4561 agcgagcttc gcccaagata tcgtcctgct caagtacgtc ggcattaatc ccgtggtggt
     4621 tcatggtggt ggtccacaaa tcgatgccgt gctcgtgaag atgggcgtca agagccacta
     4681 tgtacgcggc atgcgcgtca ccgatcagga gacgctcgat atcgtcgaga tggttctagt
     4741 cggcaaggtg aacaaagaga tcgtcaacct gatcaaccaa catggcggaa tggcggtggg
     4801 gttgagcggt aaggacggcg ggctgattct tgctaaaaag atgaatgtca ccgtcgcttc
     4861 gcccaatggc gaatcgcccg agatcatcga tattggcatg gtcggcgaaa tcatcggcat
     4921 taatccgctg gtgatcgagt ccctcgatca aaataagttt atcccggtga tcgcgccggt
     4981 cggggtcggc aagaagggcg agacctacaa tataaatgcc gatttagttg ccggcgagat
     5041 cgccgaggcg ttgcacgcgg aaaaattctt gctcatgacc gacgtcgaag gggttaaaga
     5101 caagaagggc gccctgatga gcacgctcac cgagaagcag gcgcgcaaga tgatccagga
     5161 cggcgtagtc ggctctggga tgattcccaa agtcgagtgt tgcattaacg cgctcaaagg
     5221 cggcgtcgaa aaaacccata ttatcgacgg ccgggtaaag cacgcggtgc ttctggaaat
     5281 ttttaccaag caaggcatcg gcaccgaggt cgtgagaaag taaatgaaga atagagacgt
     5341 ggctgcgctg accgacaagt tcattgccgg gacctatgcg cgcacgccgc tggcgctggt
     5401 taagggccgc ggtgtcaagg tctgggacgc cgacggcaaa gagtatctcg actttctcgc
     5461 cggcatcgcg gtcaatagtt tcggtcattg ccatccagcg atcgtcaagg cgatcaaaca
     5521 gcaagcgcaa gttctgatgc atgtgtcgaa cctttaccac atgcagccgc acgcggagct
     5581 cgcccgggaa ctgtgccgaa attcgtttgc cgaccgagta ttcttctgta acagcggtgc
     5641 cgaggccaac gaagcagctt tcaagctggc gcgccgttac gggcaggaaa agctcggcgg
     5701 caagtacgag attatctcga cgcacaactc gtttcacggc cgcacgctgg cgaccctcac
     5761 ggcgacgggc caggaaaagg ttcgcgcagg ctacgatccg ttgccccttg gctttcgcca
     5821 ggtgccgttc aacaagcttg aggcgatgga agcggccatc gacgaaaaga aaaccgtggc
     5881 gattttaatc gagccgatcc aggcggaggg cggcgtcaac gtaccgcacg agacttacat
     5941 gttgggcctg cgcgagatct gcgatcaacg acgtattctg ctgatttttg acgaggtgca
     6001 gaccggcatg ggccgcacgg gaaagctttt cgggtatgaa aacttcgggg tcaaacccga
     6061 tattatgacc ctggccaagg cgctgggcgg cggtttgccg ctaggtgcca tgttggcgcg
     6121 cgaagacgtc gcgagcagct ttaatccggg aagtcatgca tcgacattcg gcggcaatcc
     6181 agtctcgtgc gctgctggag tggcggttat gcagctgctc aagagcggcg tcctcaagaa
     6241 ctgtgtgcaa atgggcaaat acttcgtcaa aggtctggag gcactaaaaa aacgattccc
     6301 ttttattcgc gagattcgtg gcaagggctt gctcataggc gtcgagcttg agattgacgg
     6361 cagcaaaatc gtcgaatctt gcatgctcga ggggttgctg atgaattgca ccgcggcaaa
     6421 agtgctccgc ttcgtaccgc ccctgacgat caagaaaaat gaaatcgatc gcggcttagc
     6481 gagcctagaa aaagtgctgg ctcgccaata gttcaattta ccggttcatg aagaagcgcg
     6541 atctacttag tttcgacggc atgacccggc gagagtttga cgggctttta cagctcgccg
     6601 gcgaactcaa gcgcaaacaa aagcgcggca tcgctcatcg gctcttggcc ggcaaaggca
     6661 tggcgttggt gtttgagaag ccgagtctgc gtacgcgctc gacctttgct gtaggcatga
     6721 cgcaattggg gggcagctcg gtctatttag gtccatcgga ggttggcttg ggtacgcggg
     6781 aaagcccggc agactgcgcg cgaaatctcg accgatggtt cgatctcgtc acagtgcgca
     6841 ccttcgcgca taaaattatc gaagaaatgg cgaagtactc ttcggtaccg gtgatcaacg
     6901 cgctcactga tacctatcac ccttgtcagg tgttagccga ctgtcaaaca ttgattgaac
     6961 acaagggccg gttagacggt ttgaaaatt
//
LOCUS       DATLCH010000239        12979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2882916, whole genome shotgun sequence.
ACCESSION   DATLCH010000239 DATLCH010000000
VERSION     DATLCH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12979
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882916"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VMZ02_07025"
     CDS             <1..979
                     /locus_tag="VMZ02_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HUR71720.1"
                     /translation="IDKFLDDAIEVDVDALGDGDDYVIGGIMEHIERAGVHSGDSACS
                     LPPKTISREVQDEIRRHTVALARALNVRGLMNVQFAVQGSNVFVLEVNPRASRTVPFV
                     SKAIGIPLAKMAAKIMVGRKLKDLGLTREIVPTHISVKEAVFPFNKFPGVDTLLGPEM
                     KSTGEVMGIDTSFGIAFAKAQLAGGVRLPKHGKVFISVRDEDKAAVTPVAKTLDSAGF
                     DIVATKGTAAYFGSHGVSCEVVKKVQEGGPNIADRIKAGDIALVINTPEDARSHADSF
                     YIRRCALDYSVPYFTTIAGAEAAAEGIVYLQKHDFDVKPLQEYGVPVLP"
     gene            988..3201
                     /gene="recG"
                     /locus_tag="VMZ02_07030"
     CDS             988..3201
                     /gene="recG"
                     /locus_tag="VMZ02_07030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HUR71721.1"
                     /translation="MKPAVQSSENKPVAAHPQLSVRDCLRALSTSVQFLKGVGPKRAA
                     QLEAAGLATVEDVLYHLPFRYEDRRQLKKINQAAIGQEESFAGQLVMLQNRFNARRRR
                     QMLSAVLRDETGTLDLMWYRAPSYLAKGLAQGQNLLVHGKVEQGTQGRLRIVHPEFEV
                     LDDGEESDPTRVARILPVYLRPGGIPLSFMRKLATQALQQYRYSLPPGLPRTVASRMQ
                     LASIPEALSQLHGPSIDTSVSALNEANSPAHRSVIFDELFYLQLGLGLRKHSRAQSKA
                     QQVNIAGGRLVTRMLELLPFKLTNAQQRVFGEIAADMAGAQPMQRLVQGDVGSGKTMV
                     AWLASLRVIEQGYQALWMAPTEILAEQHFRGLSRYAESLGVTVAILTAATPAKARKEL
                     LARIESGEIQFVVGTHAVIQEDVHAPCMGLGVIDEQHRFGVAQRLSLRRLVAGKESLS
                     SVTHEPHMLLMSATPIPRSLAIVLYGDMEVSFVDEMPPGRTPVETRVFTERERKQVYS
                     IVLEQLRQGHQAFVVLPLVEPSEQLEQVKDATQMAEKMRETLFKDFGVGLVHGRMKVE
                     ERDQTMRAFRDGKLGILVATTVIEVGIDIANATVMVIEHAERFGLSQLHQLRGRVGRG
                     QAPGYCLLVNRGTASAVAVERLRVMAKEKDGFKIAEADLKLRGPGELLGTRQSGLADF
                     RLASLTRDTQLLIAARQEALAWLEKDPQLKMKDSAGMRDILKHRWGKRLQLGSVG"
     gene            3310..4488
                     /gene="alaC"
                     /locus_tag="VMZ02_07035"
     CDS             3310..4488
                     /gene="alaC"
                     /locus_tag="VMZ02_07035"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HUR71722.1"
                     /translation="MEFQRIKRLPPYVFSIIDGMKMEARHRGEDIVDFGMGNPDLPTP
                     PHVVAKLIEAASKPANHRYSVSRGIYKLRVAITKWYQRRYGVTIDPDSEAIVTIGAKE
                     GLSHLAWATIDPGDVVLCPSPTYPIHQYAVVLAGGDLRCIPLTTSEEFFGNLDEAIRQ
                     TWPKPKMLVISFPHNPTTQVVELDFFEKVVKTAKEHDFLVVHDLAYADLTFDGYQAPS
                     FLQAAGAKDVGVEFFSLSKSYNMPGWRVGFCVGNKEIVQALARIKSYLDYGIFQPIQI
                     AAIQALEGPQDCVGEICSMYQKRRDSLLDGLDRAGWHIPRPKGTMFVWAEIPGPAKKM
                     GSVEFCKYLLQEAQVAVSPGIGFGQYGDEHVRFALIENEHRTKQAVRKIKRALGKLLD
                     "
     gene            4775..6163
                     /locus_tag="VMZ02_07040"
     CDS             4775..6163
                     /locus_tag="VMZ02_07040"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HUR71723.1"
                     /translation="MANSLRKVGVGLLGSGVVGEAIQDILFRELKGQVGNGIELELRR
                     IYTRNPRGKKWFGKRKALFTSRPEEVLDDPRIDIVIEALGFQAATQLPQFRDYIMRAF
                     RNGKSVVTSDKAVLARYGKEIWAAAKSTGQQLRFEACVGGGIPVIRSLSESFAVEQPE
                     AVFGIVNGTCNYVLSQMEKSGKPYAEALREAQQKGYAETNPASDVNGSDAEAKLLLLS
                     MVAFGLQIKPGKIWRKGIEAIQPVDFRYAQRIGASTIKQMAVARRRGPSVQVFVSPAL
                     VPGDNFLAGVAGATNAICFAGKSSGSGRGTRDCDYVLVGPGAGGGPTAVAVLGDVCEL
                     ARGTRSFSGAPSLIAPGTLKLQREDDIDVPFYVRFVVKDRAGIVGDIGKAFGRIGVNI
                     SEIWQLRHVEEELRGLMQSYRLKQKPGEILPFVMTLERTTLRHMRRALDSIRNRDYIL
                     VDPVWFPIWNSN"
     gene            6265..7485
                     /locus_tag="VMZ02_07045"
     CDS             6265..7485
                     /locus_tag="VMZ02_07045"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF003242.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HUR71724.1"
                     /translation="MKYIILQGDGMVDYPIDALGGRTPLEAARTPNMDWLAQHGVYGI
                     AHVIPKGFPPGSDVGNMSIMGYDPAIYHTGRSPLEAASMGVALGPKDIAFRCNLVTIA
                     GSGADATMEDFTSGHISTAEAAEIIRDLDRELGDNGCEFFPGVSYRHLMVWRDGLEKM
                     ETTPPHDITDQKIFPYMPKGEGADRLLQLMNASQAILAQHPVNLKRKAEGHRQVTTMW
                     LWGQGRAPQLPPLTERFGITGGVISAVDIIHGLGVYAGLERIDVPGITGFLDTNYIGK
                     GEYGVRSLEKNDLVFIHVEATDEAGHMGDVKAKIQALEDFDEKVVGTVLKGMAQRQDY
                     RILLMPDHPTACALKTHVSEPVPFVLYSPQEERDNGAVGYNEKDALKTGVVAEQAYRL
                     MESLIEGRPTWTEI"
     gene            7470..8420
                     /gene="glpX"
                     /locus_tag="VMZ02_07050"
     CDS             7470..8420
                     /gene="glpX"
                     /locus_tag="VMZ02_07050"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HUR71725.1"
                     /translation="MDRNLALEAVRVTEAAALNCARLTGCGDEKAADQAAVDAMRKAF
                     DALSIEGTVVIGEGERDEAPMLYIGEKVGSGGPRVDIALDPLEGTTICATGGPNALAV
                     LAMADGGNLLHCPDTYMAKIACGPAGKGVIDLDKSPTENLHALAEAKHCKIEDLMVII
                     LARPRHDAIIAEVRKAGARIRLIGDGDVAAAIATTKPETGIDLLIGIGGAPEGVLAAA
                     ALRCVGGEFQGRLAPRNNEEIERAKKMGVADIKKKFRIDELAAGDVMFSATGVTDGDY
                     LRGVHFFAGGATTQSVVMRSKTKTIRVIDATHYFDHKPMY"
     gene            8483..8806
                     /locus_tag="VMZ02_07055"
     CDS             8483..8806
                     /locus_tag="VMZ02_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71726.1"
                     /translation="MRKWVEVNVGTLSMAQKKKAKKTSRLQTSLFYILVPVVIWVLSF
                     IAWLYWDNLTKVFSTDSGREQSAPRATRKLDNADKSTAPTKRSPEKILDEDRKKLEDI
                     LKQRQ"
     gene            8933..10096
                     /locus_tag="VMZ02_07060"
     CDS             8933..10096
                     /locus_tag="VMZ02_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HUR71727.1"
                     /translation="MNFWHDIPKPIIGLSPMDGVTDASFRWIAARHGGPDITVTEFTN
                     VDAVIHAPHTLIRDFTYSDLERPIIAQIYGNKPELFYKAAHVACELGFDGLDINMGCP
                     AKNVAASGSGAALIRTPELARQIIQQTRRGIDDWVAGQSLNDLGLRQKLRETVTSSNQ
                     QRSGWDTPPARQRIPLSVKTRIGYDEIVIEDWIANLLEEKPVAISIHGRTLKQGYKGD
                     ADWDAIARAVEIARPTQTLILGNGDLRDMGDVYRRVRQCGVDGVLLGRAAEGNPWIFK
                     AKAQLKQALRDSVPPAARESVDLAERFKVIVEHSHHFETYVQERNFVGMRKHLIWYCR
                     DFRGAAELRTKMVRVNGTADVIQCLRDYQAGCEAQASVARDAPQAEPSLFPVP"
     gene            10114..10743
                     /locus_tag="VMZ02_07065"
     CDS             10114..10743
                     /locus_tag="VMZ02_07065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HUR71728.1"
                     /translation="MRLILASTSPRRREILALLGVPFEVIAPDYTEVVSTHLNIEDEV
                     LEFAIGKARSVAVHHSSAIVIGSDTMIFLEGAKIGKPSDATDAKRILRLLSGKTHLIY
                     TSVAILDGCGGDGLQITSKVSVDMHRYDDEVIDNYLACNESMDKAGAYSIQGEGSKLI
                     AAIDGDFLAAVGLPLRPIAEYLRHRRIEPAVDIDGLYGAKEFLNWRQFK"
     gene            10780..11967
                     /locus_tag="VMZ02_07070"
     CDS             10780..11967
                     /locus_tag="VMZ02_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUR71729.1"
                     /translation="MASVYETTIIVLCGFWTLGFLYLFWARLRVPQFHRLQFSEAKDW
                     PLLSVIIPACNEAANIESAVESLLGQNYPNLEVILINDRSSDGTAEIIDRLALGDGRI
                     RSLHVGTLPDGWLGKVHALDRGVQLAEGDWLLFTDADVHFAAGTLRRAVGWASDNRID
                     HLALAPLAVQESFLLDIVVHTFMLLFLIGTRAAGINRPTSRAFAGVGAFNLVRRSAFQ
                     RTPGFSWLRLEPCDDVGLGLMIKRTGGWSFFAFAQKHLTVVWYPSVAAMFKGLEKNLF
                     GSTANYHWWLMLINTFGLFALALAPGLAVVGGMTKTQNILLGAGLGAMTMHWMFSLCA
                     GRQSGRSRLSMLFAPVGLMMIGAMMVHAAWKCLSHGGIDWRGTRYPLEQLRAGQRVKL
                     LSS"
     gene            12142..12339
                     /locus_tag="VMZ02_07075"
     CDS             12142..12339
                     /locus_tag="VMZ02_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71730.1"
                     /translation="MKQPNNMTQGGQNWQWPEKTWFRERLNAMKAQENQQRRRPIVNS
                     QQPTSTIPSPEAEPADLLKAE"
     gene            complement(12472..>12979)
                     /locus_tag="VMZ02_07080"
     CDS             complement(12472..>12979)
                     /locus_tag="VMZ02_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71731.1"
                     /translation="DAMSGLNMAVGVHVGNGSPHNIDMVSQYNGGGSFWKFRIPVIGA
                     FHSIIMGEIPRQFPKLRFHWAEAAANWVPYVVKDLQRRWATQKKDLPNNPLKEFRQYV
                     SCQTDDDVDYVLKYSGEDNLTIGTDYGHNDQSTEVEALANLKKTGMITDGQYDKISYH
                     NPKALFAL"
BASE COUNT         2855 a         3523 c         3758 g         2843 t
ORIGIN      
        1 gatcgacaag tttctcgacg atgccatcga agtggatgtc gatgcgctcg gcgacggcga
       61 cgattatgtt atcggcggca ttatggagca tatcgagcgc gccggagtgc actccggcga
      121 tagcgcatgc agtctgccgc cgaaaaccat cagccgggaa gttcaagatg aaatccgccg
      181 ccataccgtc gcgctcgcta gggcgctcaa cgtgcgcggt ttgatgaacg tgcagttcgc
      241 cgtgcaaggt tccaatgtct tcgtgttgga agtaaacccg cgggcttccc gcaccgtgcc
      301 gttcgtcagc aaagcgatcg gcataccgct ggcgaagatg gcggctaaga tcatggtcgg
      361 cagaaagctc aaggatctcg gcctgacacg ggagatcgtg ccgacgcata tctcggtgaa
      421 ggaagcggtt ttcccgttca ataaatttcc cggcgttgac accttactgg gtcccgagat
      481 gaagtcgacc ggcgaagtta tgggcatcga cacgtcattc gggattgcct tcgcgaaagc
      541 gcagcttgct ggcggtgtac gcctgcccaa gcacggtaaa gtctttatta gtgtacgcga
      601 cgaagacaag gcggccgtga cgccggtagc gaagaccctc gatagcgccg gtttcgatat
      661 cgttgccacc aagggaaccg ccgcatattt cggatcgcat ggagtttcct gtgaagttgt
      721 caagaaagtt caagagggcg gaccgaacat cgcagatcgg attaaggctg gcgatatcgc
      781 gctggtaatc aatactccgg aagacgcccg atcccacgcc gactctttct atattcgtcg
      841 ctgtgccttg gactattcgg tcccttactt taccaccatt gccggcgccg aagccgctgc
      901 tgaaggcatc gtctatttgc aaaaacacga cttcgacgtc aagccactgc aagagtatgg
      961 cgtgcccgtc ttgccgtagg cacgataatg aagccggcgg tccagtcctc agaaaataaa
     1021 cccgttgccg cgcatcctca gctcagcgtg cgtgactgct tgcgcgcgct gtctacttca
     1081 gtgcaatttc tcaaaggcgt cggacccaag cgtgcagcgc agctcgaagc agccggtttg
     1141 gcgaccgtcg aagatgttct ctatcacttg ccatttcgtt acgaagaccg gcgtcagtta
     1201 aagaaaataa accaggcggc gatcggacaa gaagagagtt ttgccggcca gttggtgatg
     1261 ctgcaaaatc gcttcaatgc gcgccggcgc aggcagatgt tgtcggcggt gctgcgcgac
     1321 gagaccggta ctctcgatct gatgtggtat cgggcgccga gctatttggc gaaagggctt
     1381 gcgcaagggc agaatctctt agtccatggc aaggtcgagc aggggaccca aggacgtctg
     1441 cgcattgttc atccggagtt tgaagttctc gatgacggcg aagagagcga ccccacgcgc
     1501 gttgcacgca tcttgccggt gtatttgcgg cccggcggta ttccgctgtc gttcatgcgc
     1561 aagctggcga ctcaagcgct ccaacagtac aggtactcct taccgccggg gctgccacgg
     1621 accgtggcaa gccgtatgca attggcatca ataccggagg ccttgagtca gttacatggc
     1681 ccatccattg acaccagcgt aagcgctttg aacgaggcca attcgccggc gcaccggagt
     1741 gtgattttcg acgaattgtt ctatttgcag ctgggcttgg gcctgcgcaa acattcacgg
     1801 gcgcagagca aggcgcagca ggtcaatatt gctggtggca gactggttac acgcatgctc
     1861 gaactgttgc cattcaaatt aaccaacgcg cagcagaggg tctttggcga gatcgcggcc
     1921 gacatggccg gcgcacagcc gatgcagcgc ttggttcagg gcgatgtcgg ttcaggcaaa
     1981 accatggtgg cctggttggc gtcgctgcgg gtcatcgaac agggatatca ggcactctgg
     2041 atggcgccaa cggaaatcct tgccgaacag cattttcgcg gcttgtcgcg ctatgccgaa
     2101 tctttagggg tgaccgtggc gatcttgacc gccgccacac cggccaaggc gcgcaaggaa
     2161 ttgctggcgc gcatcgagag cggcgaaatc cagtttgtcg tcggcactca cgcggtcatt
     2221 caagaggatg ttcatgcgcc atgcatgggt ctgggtgtca tcgatgaaca gcaccgcttc
     2281 ggcgttgctc aacgcttgtc gctgcgccgg ttagtcgcgg gcaaggagtc tctgtcttcg
     2341 gtaacccatg agccgcatat gctgttaatg agcgccacgc cgatcccacg cagcctagcg
     2401 atagtgctgt acggtgacat ggaagtatcc ttcgtcgacg agatgccgcc cggccgcacg
     2461 ccggtcgaaa ccagggtttt caccgaacgc gaacgcaagc aggtctattc catcgttctc
     2521 gagcaactga ggcaagggca tcaagccttt gtcgtattgc cactggttga accgtcggaa
     2581 caattggagc aagtcaaaga cgccacccag atggcggaga agatgcgcga aacgctgttt
     2641 aaagacttcg gcgtcggctt ggtccatggt cgtatgaagg tggaggagcg tgatcaaacc
     2701 atgcgggcat ttcgcgatgg caagttgggc atcctcgtcg ccaccaccgt tatcgaagtt
     2761 ggtatcgaca ttgccaacgc tacggtaatg gtgatcgagc atgccgaacg cttcggctta
     2821 tcgcagctgc atcaactgcg cggccgggtc ggccgcggcc aagcgccggg atactgcctg
     2881 ctcgtcaatc gcggcaccgc aagtgccgtt gcggtcgagc gactgcgcgt catggctaaa
     2941 gagaaagacg gtttcaaaat cgccgaagcc gatttgaaac tacgcggtcc gggtgagctt
     3001 ctcggcaccc ggcagtcggg tttagctgac tttcgtttgg ccagtctcac ccgcgacacg
     3061 caattgctca tcgccgcgcg tcaggaagcc ctcgcgtggt tggagaaaga tccccagctc
     3121 aaaatgaaag actcggccgg catgcgcgat attcttaaac atcgctgggg taagcgattg
     3181 caattgggct cggtcggata acgctggtca attccttgtg cttcaaagtt tctcatttgc
     3241 acccgcggta gcgatgcctt ataaatataa aaacttttag ttctctaaat caaaaagagg
     3301 ttacggcgag tggaatttca gcggattaag cggctgccgc cctacgtctt cagcattatc
     3361 gatggcatga agatggaggc gcgacatcgg ggcgaagata tcgtcgactt cggcatgggc
     3421 aatcccgatt tgcccactcc acctcatgtg gtggcaaagt tgatcgaggc cgcatcgaaa
     3481 cccgccaatc accgctattc ggtctcgcgc ggcatctata agctacgggt cgccatcacc
     3541 aaatggtacc agcggcgcta cggcgtgacc atcgatccag acagcgaggc gatcgtaacg
     3601 atcggcgcta aagaagggct gtcgcatctt gcttgggcca ccatcgatcc cggcgacgtg
     3661 gttctctgtc cgagtccgac ctatccaatt caccagtatg cggttgtgtt agcaggcggc
     3721 gatctgcgtt gcattccgct gactaccagc gaggagtttt tcggcaatct cgacgaggcc
     3781 atccgccaga cctggcccaa gcccaagatg ctggtcatca gctttccgca taatccgacc
     3841 acccaggtgg ttgaactcga ttttttcgag aaggtcgtca aaacggcgaa ggaacacgac
     3901 ttcttggttg tccacgatct tgcctatgcg gatctgactt tcgacggcta ccaggcaccg
     3961 agctttctgc aagcggcggg agccaaggat gtcggcgtag agtttttttc gttatccaag
     4021 agttacaaca tgccgggttg gcgggtaggg ttttgcgttg gcaataagga gatcgtccag
     4081 gccttagcgc ggatcaagag ttatttggat tatggaattt ttcaaccgat ccagattgcc
     4141 gccatccagg ccctcgaagg tccgcaggat tgcgtcggtg aaatttgctc gatgtatcaa
     4201 aaacggcgtg actcgctgct cgacggccta gaccgtgcgg gttggcatat cccgcgcccc
     4261 aagggcacga tgtttgtgtg ggcggaaatc cccggacccg cgaagaagat gggctccgtc
     4321 gagttttgca aatatttgct tcaggaagcc caggtggcgg tctcgccggg cattggcttt
     4381 ggtcagtacg gcgacgaaca tgtgcgcttc gcattgattg aaaacgagca tcgtaccaag
     4441 caggccgtgc gaaaaatcaa gagagcgctc ggcaagctgc tcgattaata gttcacgaag
     4501 tctcatctcc ttcgtgcctc cttagaaaat agttgtgcag ccacgatcgg gcgacgaatt
     4561 gaacaaacgt ttcgttatac cgacgaaagc ggtatccctt ctgcccttga cgccggtttt
     4621 cgccggccta ccgactaagg gaaagaacgc tgttcgaagc gggttctttg caattccttc
     4681 tctgtgacga gcgtaagcta atgaacacct cgtggtgcaa aacacaagtc gacactcaca
     4741 cgaagctctg aagaactgac gcgagtaaaa cagcatggcg aattctctac gcaaagtcgg
     4801 cgtcggactg ctcggctcgg gagtggtcgg cgaagcgatt caggatattt tattccgcga
     4861 gctcaaggga caggtcggca acggcatcga gcttgagctc cggcggattt acacgcgcaa
     4921 tccccgtggc aaaaaatggt tcggcaagcg caaagcgttg ttcaccagtc ggcctgaaga
     4981 agtactcgac gatccgcgca tcgatattgt gatcgaagcc ttgggcttcc aagcagcaac
     5041 gcagctgccg cagtttcgcg attacataat gcgcgccttc cgcaacggca agtccgtggt
     5101 gacctccgac aaggccgtgt tggcacgcta cggcaaggag atctgggcgg cggcgaaaag
     5161 caccggacag caattgcgtt tcgaagcctg tgtcggcggc ggcattccgg tgatccgttc
     5221 gctgagcgag agctttgctg tcgaacaacc cgaagcggtg ttcgggatcg tcaatggcac
     5281 ctgcaattat gttctctcgc agatggaaaa gagcggaaag ccgtacgccg aagcgttgcg
     5341 cgaagctcag caaaaaggct acgccgaaac caaccctgcg tctgatgtca acggcagcga
     5401 cgctgaagcc aagctgctat tgctcagcat ggtcgcgttc ggtttgcaga tcaaaccggg
     5461 aaagatttgg cgcaaaggca ttgaagcaat tcagcccgtc gattttcgct atgcccaacg
     5521 catcggcgcc agcaccatca agcaaatggc agtggcgcgc cggcgcggtc cgtctgtgca
     5581 ggtctttgtc tcgccggctt tggtgccggg agataatttt ctcgccggcg tcgccggcgc
     5641 gacaaacgct atctgtttcg ccggcaagag cagtggcagc ggacgcggca cacgggattg
     5701 cgattatgtt ctagtggggc ccggcgccgg cggcggtcct acagccgtgg ccgtgctcgg
     5761 agacgtctgt gagctggcgc gcggcacgcg cagcttcagc ggcgcgccaa gtttgatcgc
     5821 gccgggcacg cttaagctgc aacgtgaaga cgatatcgat gtgccgtttt atgtccgctt
     5881 cgtggtcaaa gaccgcgccg gcatagtcgg cgatattggc aaggccttcg gccggatcgg
     5941 cgtaaatatt tccgagattt ggcagctgcg ccatgtcgaa gaggagctgc gcggtttgat
     6001 gcaaagttac cggctcaaac aaaagccggg cgagattttg cccttcgtca tgaccttgga
     6061 gcgcacgact ttgcggcaca tgcgccgggc gctggattct attcgcaatc gtgactatat
     6121 cttagtcgat cccgtgtggt ttccgatctg gaattccaac tagttatccg agatatgaga
     6181 ctgccggtga aactctgtcg tgaaaccctc ttcagacgaa accccacaga agcaacagta
     6241 gatgaactta aggtggttcg atcgatgaaa tacataattc ttcaaggcga tggcatggtt
     6301 gactatccca tcgatgctct cggcggcagg accccgttgg aagcggcgcg tacgcccaac
     6361 atggactggc tggcgcaaca tggcgtctat ggcatcgccc atgtcattcc taaaggtttt
     6421 ccgcctggca gtgatgtcgg taacatgagc atcatgggct acgacccggc aatttatcat
     6481 accggccgtt ctcccctcga ggccgccagt atgggtgtgg ctcttggccc aaaagatatc
     6541 gcttttcgtt gcaacctggt caccatcgcc ggtagcggtg ccgatgcgac gatggaagat
     6601 ttcacttccg gtcacatctc caccgcagag gcggcggaaa tcatccgtga cctggatcgt
     6661 gaactcggtg acaatggctg cgaatttttc cctggcgtga gctatcgcca cctgatggtt
     6721 tggcgcgacg ggttggagaa aatggaaact acgccgccgc atgatatcac tgatcagaaa
     6781 attttccctt acatgcccaa aggagaaggt gccgaccgcc tcctccaatt aatgaatgct
     6841 tcgcaagcaa tattggccca gcatccggtg aatctaaaac gcaaggccga aggccatcga
     6901 caagtgacga cgatgtggct gtggggacag gggagggcgc cgcaattgcc gccgctgacc
     6961 gaacgtttcg ggattacagg cggcgtcatt tcggcggtcg atattattca cggtctgggc
     7021 gtctacgccg gtttggaacg catcgatgtt cccggcatca ccgggtttct cgatacgaat
     7081 tacatcggca agggcgagta tggtgtgcgc tcattggaaa agaacgattt agtttttatc
     7141 catgtcgagg ccaccgatga ggccggccat atgggggacg tcaaggcgaa gatacaggcg
     7201 ctggaagact tcgacgagaa ggttgtcggc acggttctta aggggatggc gcaacgtcaa
     7261 gactatcgca ttttgctcat gcccgatcat ccgactgctt gcgcgctcaa aactcatgtt
     7321 tccgaaccgg ttccgttcgt gttgtattcg ccgcaagagg agcgcgacaa cggcgccgtc
     7381 ggttataatg aaaaagatgc cctgaagaca ggagtcgtcg ccgaacaagc gtaccgactc
     7441 atggagtcgt tgatcgaagg gagaccgaca tggaccgaaa tctagctcta gaagcagtgc
     7501 gtgtcaccga agcggcagcg ctcaactgcg ctaggctgac gggttgcggc gacgagaaag
     7561 cggcagatca agctgcggtc gacgccatgc gcaaggcctt cgatgcacta tccatcgaag
     7621 ggacggtggt gatcggcgag ggtgaacgcg acgaggcgcc gatgctatat atcggcgaaa
     7681 aagtcggctc cggaggcccg cgggtcgata tcgcgcttga ccccttggag ggcacgacca
     7741 tctgcgctac gggcggccct aacgccctgg ccgtgctcgc gatggccgac ggcggcaatc
     7801 tcttgcactg cccagatact tatatggcga agatcgcctg cggcccggcg ggcaaggggg
     7861 tcatcgattt agataaatca ccgaccgaga atcttcatgc cttggcagaa gccaaacatt
     7921 gcaagataga agatttgatg gtgattatcc tcgcccgacc gcgtcacgac gcgatcattg
     7981 ccgaagtgcg caaagcgggg gcgcgtattc gcttgatcgg cgatggcgat gttgccgcgg
     8041 ccatcgcgac caccaaaccg gaaaccggca tcgacctact gatcggcatc ggcggggcac
     8101 ccgaaggcgt attggcggcg gcggcgctgc gctgtgtcgg cggcgagttt caagggcgat
     8161 tggcaccgcg caacaatgag gagatcgagc gggcaaaaaa aatgggcgtc gccgatataa
     8221 agaagaaatt tagaatcgac gagttagccg cgggcgatgt tatgttttcc gccactgggg
     8281 taaccgatgg cgattatctg cgcggcgtcc atttctttgc cggcggcgcg actacgcaat
     8341 ccgtggtcat gcgctccaag actaaaacca ttcgggtgat cgatgcgacg cactatttcg
     8401 atcacaaacc aatgtactga tcgaatcgcc aaacccaata caattcgtca gcgcacagac
     8461 aacgacggtc agcctcgctc acttgcgcaa gtgggttgag gttaacgtcg gcacgctcag
     8521 catggcccag aaaaagaagg caaaaaaaac cagtcggctc caaactagct tgttctatat
     8581 tctcgttccc gtcgtcatct gggttctatc ctttatcgca tggctgtact gggacaatct
     8641 cacaaaggtg tttagcacgg actcgggcag agagcagagc gctccgcgcg ccacccgcaa
     8701 gctcgacaac gctgacaaga gcacggcgcc aaccaaacgg tctccggaaa aaatccttga
     8761 tgaggaccgc aaaaaactcg aagatatttt gaagcagcgc cagtagtctt tcactgtgtg
     8821 tagtgactat cttagcaacg ctaagcacgt cactggaata ccttgttgat ggtccttggt
     8881 cggtaaatga cgatggattt tcttcgaagg cgatgtaaga gtaaatcacg ttatgaactt
     8941 ttggcacgac atacccaagc cgatcatcgg cttgtcgcct atggatggcg ttacggatgc
     9001 gtcctttcgc tggatcgccg cacgacatgg cggcccggat atcaccgtca ccgagttcac
     9061 caacgttgac gcggtgatcc atgcgccgca cacgttgatt cgagatttta cctattccga
     9121 cctcgaacga ccgattatcg cgcagatcta cggcaacaag ccggaacttt tttataaagc
     9181 ggcgcatgtc gcctgtgaac taggtttcga tggactggat atcaacatgg gctgtccggc
     9241 caagaatgtc gcagcatcgg gcagtggtgc tgccctaatc cgtacgccgg agttggcgcg
     9301 acagatcatc caacagacac ggcgaggcat cgatgattgg gtggccggtc agagcctcaa
     9361 cgatttgggt ttgcgtcaga agctccgcga gacggtcaca tcctccaatc aacagcgtag
     9421 cggctgggat accccgccgg cacgccaacg aataccgctg tcggttaaga cccgcatcgg
     9481 ctacgacgaa atagttatcg aggactggat cgccaacttg cttgaagaga agccggtggc
     9541 gatctcgatc catggacgca cgctcaagca gggctacaag ggcgatgctg attgggacgc
     9601 catagctcgc gcggtggaga tcgcccggcc gacgcaaacg ctcattctgg gtaacggtga
     9661 tctgcgcgat atgggagatg tttatcgccg cgttcgtcaa tgcggtgtcg acggagttct
     9721 tctcggccgg gctgctgaag gaaatccttg gatttttaaa gccaaggcgc agttgaaaca
     9781 ggctttgcgc gattcagtgc cgccggcggc cagggaatcc gtcgacctag ccgaacggtt
     9841 taaagttatc gtcgagcact cgcatcattt tgaaacctac gtccaggaac gcaacttcgt
     9901 cggaatgcgc aaacacttga tctggtattg ccgcgatttt cgcggtgccg cagagttgcg
     9961 caccaaaatg gtgcgcgtca atggaaccgc cgatgtcatc caatgtctac gcgattacca
    10021 agcggggtgt gaagcgcagg cgagcgttgc gcgggacgcg cctcaagcgg agccaagcct
    10081 atttcccgtg ccctgatgcc ttgtgaatcc taaatgcgac tgattctcgc ttcgacttca
    10141 ccccgacgtc gggagatttt ggcgctgttg ggcgtgccgt tcgaggtgat cgctcccgat
    10201 tataccgaag tcgtttctac tcacttgaac atcgaagacg aagttctcga atttgccatc
    10261 ggcaaggcgc gatcggtcgc tgtccatcat tcatcggcga tcgtcatcgg cagcgatacg
    10321 atgatctttc tcgagggcgc caagatcggc aaaccgagtg atgccaccga tgccaaaaga
    10381 attttacgtc tgttgtcggg caagactcat ttgatctaca cgagtgtggc gattctcgat
    10441 ggctgcggcg gtgacggatt gcaaataacc tccaaagttt ccgtcgacat gcatcgctat
    10501 gatgacgagg tcatcgataa ctatctcgcg tgcaatgaat cgatggacaa agccggtgcg
    10561 tactcgatcc agggcgaggg gagtaagctc atcgccgcca tcgatggcga cttcctcgcg
    10621 gccgtgggtt tgccgctgcg gcctattgct gaatacttgc gccatcggcg tatcgagccg
    10681 gcagtggata tcgacggtct ttacggcgct aaagaattcc tcaactggcg ccagttcaag
    10741 tgaacattcc gctgcgcgcc gacttcactc ctgtgaacta tggcttcagt atacgagact
    10801 acaattattg ttctctgtgg attttggact ctaggatttc tctatctatt ctgggcacgc
    10861 cttcgggtac cgcagtttca tcggcttcaa ttcagcgaag cgaaagattg gcctttgctc
    10921 agcgtgataa ttcccgcgtg caatgaggcg gctaacatcg agagtgccgt cgagtctctg
    10981 cttggacaaa actatcccaa tctcgaagtg attctgatca acgaccgttc cagcgatggc
    11041 acggcagaaa ttatcgatcg cttggcgctg ggcgacggac gtattcgctc cttgcatgtc
    11101 ggaactttgc ccgatggttg gctcggtaaa gtccatgctc ttgaccgcgg tgtccagctt
    11161 gccgaaggcg attggctatt attcaccgat gctgatgtgc attttgctgc cggcacactg
    11221 cgccgggcag tcggctgggc gagcgacaat cgtatcgacc atctcgccct tgccccattg
    11281 gcggtgcagg agagtttcct gctcgatatc gtcgtgcaca cgttcatgtt gctgtttttg
    11341 atcggcacca gagcggcggg gattaaccgg ccgactagtc gtgcctttgc cggcgtcgga
    11401 gcgttcaatc tagtgcgccg ctcagctttt cagcgcaccc cgggtttttc atggctccgc
    11461 ctcgagccct gcgatgacgt tggtttaggg ttgatgatca agcgcaccgg cgggtggtct
    11521 ttttttgctt tcgcgcaaaa acatctaacg gttgtttggt acccatcggt tgcggcgatg
    11581 ttcaagggtc tcgagaagaa tctcttcggc tcgaccgcga attatcactg gtggttaatg
    11641 ctaattaata ccttcgggct ctttgcgttg gcactggccc cgggtcttgc tgtcgttggc
    11701 ggtatgacaa aaacccagaa tatattgctt ggcgcaggac ttggcgccat gacgatgcac
    11761 tggatgttca gcttgtgcgc tggaaggcaa tccgggcgca gccgcttaag catgctgttt
    11821 gctccggtgg ggctcatgat gattggcgcg atgatggttc atgccgcatg gaaatgccta
    11881 tctcacggcg gcatcgattg gcgcggtacc cgctacccgc tggagcaatt gcgcgccggc
    11941 cagcgcgtaa agcttctctc gagctaatcg gacgttcccg ggcgatgaca taattgtata
    12001 gcggggtaat tagcggcccc aaaactgcgc gggcgtcggg aatattcgtc gatttactag
    12061 cgtcaaggct cccgaaatta ctttcaaagt attggcttgc ggctggtcta tcgattgctc
    12121 actaagacca acaggattgc catgaaacag ccaaacaata tgactcaagg cgggcaaaac
    12181 tggcagtggc cggagaagac ctggtttcgt gaacggttga atgccatgaa ggcgcaggag
    12241 aatcagcaac gacggcgccc aatcgtgaat tcccaacaac caacgagcac gattccttcc
    12301 cccgaagccg agccagccga cttgctcaaa gctgaatagg cggctcgcaa ttcagcgagc
    12361 taagttccgg gccgcagggt gtaagcctcg gcccgcgcaa ccgccaatct cccgcaatag
    12421 ttcccatcgc gaaacgcagt aactctgcgc accgctgccc tgaatttcgc attacagggc
    12481 aaacaacgcc ttggggttgt gataactgat cttgtcgtac tgaccgtcgg taatcatacc
    12541 ggtcttcttc aaattggcca gcgcctcgac ttcagtcgat tgatcgttgt ggccgtagtc
    12601 cgtgccaatt gtaaggttgt cctcgcccga atatttaagc acgtagtcga catcgtcgtc
    12661 ggtttggcag gaaacatact ggcggaattc tttgagcggg ttgttcggca gatctttttt
    12721 ctgcgtcgcc cagcgccgct gtaaatcctt tacgacatag ggtacccagt tcgccgccgc
    12781 ttcagcccaa tgaaaacgca gcttgggaaa ctggcgcgga atttcgccca tgatgatcga
    12841 atgaaaagcg ccaatcaccg gaatgcgaaa tttccagaag ctgccgccgc cgttgtactg
    12901 cgaaaccata tcgatgttgt gcgggctgcc gttgccgaca tgcacaccga cagccatatt
    12961 cagtccgctc atcgcgtcg
//
LOCUS       DATLCH010000240         7269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5860562, whole genome shotgun sequence.
ACCESSION   DATLCH010000240 DATLCH010000000
VERSION     DATLCH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5860562"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VMZ02_07085"
     CDS             complement(<1..248)
                     /locus_tag="VMZ02_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71732.1"
                     /translation="MLLINNQQVEKLFSMKDCLAALEDGYDDLMIGDAVYRPRLDLWM
                     PCERPDGYWRWGTMEGASRKIGVFAIRMKSDVVYWPGG"
     gene            636..1547
                     /locus_tag="VMZ02_07090"
     CDS             636..1547
                     /locus_tag="VMZ02_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR71733.1"
                     /translation="MAQNRVPSDEQPSDATGLHQRALGFFKQNDHESALEDLHTAIRV
                     NPHYTPAYFLRGQIFAKKKQFAAAITDFTSVIAQDASGATAFHQRALVRQASRDHRQA
                     LEDFDRALALEPHNALILRDRAIAQRDAGNYDQAVDDYEEATRMGFSAVDPHPLAELL
                     FFQGRFHQSAQTLQQVVRTRPDNRHAFLWRYLALGKANDERGAARELSEHAARTSPDK
                     QWPAAVLDFYLGRIDEGSLYVAAEAADDLLKTEQQCQANFYIAEAKLLKGAKEDALSR
                     LQTAKSQCSTNSPFFHGANAELKRFGQ"
     gene            1754..2665
                     /locus_tag="VMZ02_07095"
     CDS             1754..2665
                     /locus_tag="VMZ02_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HUR71734.1"
                     /translation="MKILRFNDDQIGVIKNDNMVVDVSSTISARKAKGPQRVMEEIIE
                     GWRKYRKRFEKVVAEQDGVPLASVQLRSPLARPGKCIAAFSNYIDRPERTADNFPNEY
                     FYKSPDLVGPDGEVELRDIPSCVVYQPEAEFAFVMGKTAKDVPEKKALDYVFGYVPMF
                     DISTRGMVRRTQFVGKGQGSHGCCGPWIITKDEIPDPHNVNVKSWTNGEPRQNYNTSA
                     MAHKIPNQIAWLTKLLTLSPGDVVLTGTFHEGLAPVNSGDTIEIEFADMGRAKFKVVG
                     NSPRKDVPWLPGKNQPTPPPGGGMHIV"
     gene            complement(2755..3552)
                     /locus_tag="VMZ02_07100"
     CDS             complement(2755..3552)
                     /locus_tag="VMZ02_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR71735.1"
                     /translation="MASRHDGAAGGVMRLGRFNGIAFLLALLALWELLAQAQLINALI
                     IPPPSKILGIFFDLLWSGQIPLQILVSMKRALAGYLLAAAVFIPLGIFMGLSPTVYRF
                     FEVIVEMLRPVPPPVMIPVALLFFGLGDEMKVFVIFFSCAWPILLNTIDGVRGVDWVL
                     LNTARSFGLSRGKIIWQVILPACSPQITTGLRVSLPIMLILVVISEMVGSTDGIGYFV
                     LDSQRRFKVAQMYAGMLALALLGYTLNQLFNFLQRLLLPWQRGRQLE"
     gene            complement(3516..4181)
                     /locus_tag="VMZ02_07105"
     CDS             complement(3516..4181)
                     /locus_tag="VMZ02_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR71736.1"
                     /translation="MSRILGALGRALAAGSITQHGLVTLGRFAEGYLLSAVLAVSLGA
                     LLGYFRAFHSLLATLIELLRPMPSVAIIPVAILLLGIGDAMIVSVTIYASVWPILINT
                     IDGVRNIDHTLIDTGRTFGLSRWRILWEIILPGASPYIVTGLRIGLSIALILVTTAEM
                     IAGSKGLGFFILDEERAMNSANMYAGIVVVALLGYALNGLFLLAETYAMKWRHGMMAR
                     QAA"
     gene            complement(4292..5068)
                     /locus_tag="VMZ02_07110"
     CDS             complement(4292..5068)
                     /locus_tag="VMZ02_07110"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR71737.1"
                     /translation="MPILDVQSLSKSYRQAGGESTLAIGNITCQVESGEFVSFVGPSG
                     CGKTTLLMSIAGLMAPSSGRVVVNGREVHGPPRNLVLLFQEYNKSLFGWRSVLGNVRF
                     GLEARGDRSAAAENEARKLIDLVGLKGFERHYPWELSGGMQQRVAIARALAYEPEVLL
                     MDEPFGSLDALTRLELEDTLLRLWAELKTTIVFITHDIEEAIYLSDRIWILSRRPSQI
                     MQELKIEFSRPRHQVTTRAEARFMELRNDIYRQIGNQGIS"
     gene            complement(5154..6116)
                     /locus_tag="VMZ02_07115"
     CDS             complement(5154..6116)
                     /locus_tag="VMZ02_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71738.1"
                     /translation="MKVSRRNFLTKITVGIAGLSLAPDFAWAQVKGKLGYMKIVDTTP
                     MFMAMEKGFFKAEGIELETVPMAGGAVIVQGVTSGDLQFGWTNVISLYQAHVEGFDFK
                     FIAGGATNVRGSNETHAIIVGKDSPIRSAKDLEGKTVAVNTLNNIVHLMALAWVDKNG
                     GNSSKVKFVEIPFPQMEPSLVAGKVDAISVQEPFAAVAARRPETRVLANPWGDVLPRF
                     LIATWFASDKWIQKNRAVAQGFVRAMNRGVDAVNTDKDGARAAMIKWAGLNPDFAGKI
                     GLPQFDKTIAEKDLQVTMDLTYKYKMISKPLKAKDVLSDLATKA"
     gene            complement(6222..>7269)
                     /locus_tag="VMZ02_07120"
     CDS             complement(6222..>7269)
                     /locus_tag="VMZ02_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HUR71739.1"
                     /translation="LSVHGYSIGAVTSNGDASDALQGLRVGLHLTENMAYGFHLFSWL
                     QRLGVYPTRFSGGMYQDISRMLPFLPFPKHASLDGNVEDTVAAVKHMMGRLAPPFFLF
                     VHIQEPHWPYQIREGSRRVPGGNVARSQLSVSAPYRSELQPLVDTYRDSYERTIKTLD
                     AELGKFLEETSKVENSMVILTSDHGESFERGFFLHGDELYESSTWAPLLIRYPGQKNG
                     ERVSGLTQSLDLAPTILDAVGIPRPDWMEGQPLKFGASPVKAETIAINYKYSYDGVVH
                     HLPTKIAIWWDKYKLIAPCQSPEALLYDLQRDPDELVDIAAGESGIVDDLKRRLKARL
                     SRQSSEPRVSCPSL"
BASE COUNT         1766 a         2095 c         1908 g         1500 t
ORIGIN      
        1 tcgccgccgg gccagtaaac cacatccgat ttcatgcgga tggcgaacac accgatcttg
       61 cggctagcac cttccatggt gccccagcgc cagtagccat cgggccgttc acagggcatc
      121 cataagtcca agcgcggacg gtagacagcg tcgccgatca ttagatcgtc ataaccgtct
      181 tcgagcgcag cgagacaatc tttcatcgaa aacagttttt caacttgctg attattgatc
      241 agtaacatta accctccgca ttgacgacgt tgtcggaaca aaactcgacg ctctaattct
      301 atacgctgca gtaccgccga cattcaagtc attgcgatca cgcaagctaa tccctgcagg
      361 agcacagagt cattgctaca aatttgccca gccgtcgtgt aagttgtcac gctgcccatg
      421 actacccaca ccgaaatcgg cagaatttgg caggtttgaa gaatcggatc tgacgaatcc
      481 ccagttgcgg gccagaacca gcgtggcacc gatgttgctc gctcacagaa ccccggtcca
      541 atccaacttt tttaatgagc acgatgcaca actttctatc gctccttgta ttctctgctt
      601 cacttcagct gagccagatc gtattcgctg ggggcatggc gcaaaaccgg gtgccgagcg
      661 acgaacaacc aagcgatgcc accggcctgc accagcgcgc gctggggttt tttaaacaaa
      721 acgatcacga gagtgcttta gaagatctcc acacggcgat tcgagtcaat cctcactata
      781 cacccgccta ttttctgcgc ggccagatct tcgccaagaa aaaacagttc gcggcggcga
      841 ttactgactt tactagcgtg attgctcagg acgcatccgg cgccactgct tttcaccagc
      901 gcgcgcttgt gcgccaggca agtcgcgacc atcgtcaggc actcgaagac ttcgataggg
      961 cgcttgcgct cgaaccgcat aatgctctga tcttgcgtga tcgcgccatc gcccagcgcg
     1021 acgccggcaa ctacgatcaa gccgtggacg actacgaaga ggcgacgcgc atggggttta
     1081 gtgccgtcga tcctcatccg cttgccgaac tgctattttt ccaaggccgt tttcaccaaa
     1141 gcgcccaaac gctgcagcaa gtggtccgca ccaggcccga caaccgtcac gctttcctct
     1201 ggcgttatct cgccctaggt aaagccaacg acgagcgtgg cgcagcacgc gaactcagcg
     1261 agcatgcagc acggacttcg ccagataagc aatggcctgc cgcggtgttg gatttctatc
     1321 ttggcaggat cgacgaagga tcgctttacg tcgccgccga agcagctgac gatctgctga
     1381 aaacagagca gcagtgccaa gccaattttt atatcgctga agccaaactt ctcaaaggcg
     1441 ccaaagagga tgccctttcc cggctgcaaa cagccaagag tcaatgttcg accaacagtc
     1501 cgttttttca tggcgccaat gccgaattga aacggttcgg tcagtaggcc ttataccttc
     1561 ccattaacaa tcgtcgtgcg gctctactgc ggtggcggtt gtcatccaaa gctcctcctg
     1621 gttcgtgtct gacactcgga tttctaaatg gccgcgctca ctccggttca atacgcggac
     1681 gacgattttg agtttgacaa gcccatccgg ttgtcgcaaa gataggccga aatttggccg
     1741 agaggagact aacttgaaga ttcttcgttt taacgacgac cagatcggcg tcatcaagaa
     1801 cgacaacatg gtcgtggatg tcagcagcac catcagtgcg cgcaaagcca aagggccgca
     1861 gcgggtcatg gaagagatca ttgaaggctg gcggaaatat cgaaaacggt ttgaaaaagt
     1921 tgtcgctgag caggacggag tgccgctcgc aagcgtgcaa ttgcgcagtc ccctagcgcg
     1981 gccaggcaaa tgcatcgccg ccttcagcaa ctacatcgac cggccagagc gcactgccga
     2041 taattttccc aatgaatact tttataaatc tccggaccta gttggccccg acggcgaagt
     2101 cgagctgcgt gacattcctt cttgcgtggt ttatcaaccg gaggccgagt ttgcctttgt
     2161 catgggtaaa accgccaagg acgtgcctga gaaaaaagct ctcgattacg tgttcggcta
     2221 tgtgccgatg ttcgacatat cgacgcgcgg catggtgcgg cgtacgcagt ttgttggcaa
     2281 aggccaaggc tcccatggct gctgcggccc atggataatc accaaggacg aaatccccga
     2341 tccgcacaat gtcaacgtca aatcttggac caatggcgag cctcggcaaa actacaacac
     2401 cagcgccatg gctcataaga ttcccaacca gatcgcctgg cttaccaaac tgctcacgct
     2461 cagccccggc gatgtcgtcc tcaccggaac gtttcacgaa ggtctcgcgc ctgttaactc
     2521 gggcgacacc atcgaaatcg agttcgctga catggggcgc gccaagttca aagtcgtcgg
     2581 caacagtccg cgcaaagacg tgccctggtt gcccggcaaa aatcagccga ccccacctcc
     2641 cggcggcggc atgcacatcg tctagagaaa agaaggagtg atggagtagt cgactggtgg
     2701 gtttccgaca accattattc catcactcca atactccata aattactcca tcaatcattc
     2761 caattgcctg ccgcgctgcc acggcagcag caaccgctgc aaaaagttga acagctgatt
     2821 gagcgtgtag ccgagtaacg ccagggcgag catgccggcg tacatctggg cgaccttgaa
     2881 gcggcgttgc gaatcgagga cgaagtagcc gataccgtct gtgctaccga ccatctccga
     2941 gatcaccacg agaatcagca tgatcggcaa acttactcgc agcccggtcg ttatctgcgg
     3001 cgagcacgca ggtaaaatca cttgccaaat aattttaccg cgcgacaggc caaagcttcg
     3061 cgcggtgttt agcagcaccc agtcgactcc gcgcacaccg tctatggtgt tgagcagaat
     3121 cggccaggcg cacgagaaaa aaatcacgaa gaccttcatc tcgtcgccca agccgaaaaa
     3181 caagagcgcc accggaatca tgaccggcgg cggcactggc cgaagcatct cgacgatgac
     3241 ttcgaagaac cggtaaaccg tcggcgacag tcccatgaaa attcccaatg gaataaacac
     3301 tgcggcggcg agtagatagc ccgccaacgc tcgcttcatg ctgaccagga tctgcagggg
     3361 gatctgcccc gaccacagca gatcgaaaaa tattccgagt attttcgacg gcggcggaat
     3421 gatcagcgcg tttatcaatt gcgcctgagc aagcaactcc cagagcgcga gaagcgcgag
     3481 tagaaacgcg atgccgttaa accgtcccag ccgcatcacg ccgcctgccg cgccatcatg
     3541 ccgtgacgcc acttcatcgc gtaagtttcg gccagcagga acagtccatt gagcgcatag
     3601 cccaacaaag cgacgacgac tatgccagcg tacatattgg cactattcat cgctcgctct
     3661 tcatcgagga tgaaaaagcc cagaccttta cttccggcga tcatctcggc cgtggtgacg
     3721 aggatcagcg cgatcgacag accgatacgc agtccggtga cgatataggg cgacgcgccg
     3781 ggtagaatga tttcccagag gatgcgccag cggctaagac cgaaggtgcg gccggtatcg
     3841 atcagcgtgt ggtcgatgtt acgcacgcca tcgatggtat tgatgagaat tggccagacg
     3901 ctggcgtaga tcgtgacgct gacgatcatg gcgtcgccga tgccgagcaa gagaatcgcc
     3961 accggaataa tcgccaccga cggcatcggc cgcaacaatt cgatcaacgt cgccagcaag
     4021 ctatgaaacg cacgaaaata accgagcagc gcgcccaatg acaccgccaa cactgcggaa
     4081 agcaaataac cttcggcaaa gcggcctagg gtgaccagcc catgttgagt aatcgaaccg
     4141 gcagcaagcg cacggccgag ggcgcctaga atccgactca ctggcggtaa atagagttcg
     4201 agcttagggg ttgcgcgcgc gactagttcc cagacgagaa gcaacgaaac gaagcttgac
     4261 cagcccagcc agttaacacg ctgcaaaaga ttcacgatat tccttgattg ccgatctggc
     4321 ggtaaatatc gttgcgtagc tccatgaagc gcgcttcggc gcgggttgtt acttgatggc
     4381 gcggccggga aaattcgatt tttagttctt gcatgatctg cgacggccgt cggctcaata
     4441 tccagatgcg gtcggaaaga tagatcgctt cttcgatatc gtgggtgatg aagacgatgg
     4501 tggttttgag ttccgcccag aggcgcagca gggtgtcttc gagttcgagc cgtgtcaaag
     4561 catcgagcga gccgaacggc tcatccatca acaacacttc gggctcatag gcgagtgccc
     4621 gggcgatcgc cacacgctgc tgcatgccgc cggagagctc ccagggatag tggcgttcaa
     4681 accctttcaa gccgaccaga tcgatcagct tgcgagcttc gttctcagct gccgccgacc
     4741 gatcgccgcg cgcttccaaa ccgaatcgca cattaccaag caccgaccgc caaccgaaca
     4801 gcgatttatt gtactcctga aacagcaaca ccaagttgcg cggcgggccg tgaacttcgc
     4861 ggccattgac cacaacccgg ccggaactcg gcgccatcag cccggcaata gacatcaaca
     4921 gcgttgtctt accgcagccc gacggaccga cgaaagaaac gaactcgccg ctctcgacct
     4981 ggcaggtgat gttaccgatc gccaacgtag attcgccgcc ggcctgccgg tagcttttcg
     5041 aaagggattg aacgtcgaga ataggcactg gaagagattt gggatgcttt tagttgcgca
     5101 tttaccagta gctattttcg cgaacccaac actcctttat tccagtccga tcctcatgct
     5161 ttcgttgcga gatcgctcag cacgtcctta gctttgagcg gcttggaaat catcttgtat
     5221 ttgtaggtca gatccatggt gacctggaga tctttttcgg caatcgtttt gtcgaattgc
     5281 ggcaagccga tcttgccggc gaagtcgggg ttgaggccgg cccatttgat catcgccgcg
     5341 cgggcgccgt ctttatcggt attaacggca tcgacgccgc gattcatcgc acgcacgaag
     5401 ccctgcgcca ccgcgcgatt tttttggatc catttatctg aagcgaacca agtggcgatc
     5461 agaaagcgcg gcagcacgtc gccccaagga ttggccaaaa cgcgcgtttc aggacggcgc
     5521 gccgccaccg cggcgaacgg ctcctgcacg ctgatggcgt caactttgcc ggcaaccagg
     5581 gaaggctcca tttgcgggaa aggaatctcg acgaacttga ccttgctcga attgccgccg
     5641 ttcttgtcaa cccaggcgag cgccatcaaa tggacgatat tgttgagcgt gttaaccgcg
     5701 acggtcttgc cttcaaggtc cttggcactt ctgatcggcg aatccttgcc gacaataatt
     5761 gcgtgggttt cattgctgcc gcgcacattg gtcgcgccgc cggcgatgaa cttgaagtcg
     5821 aagccctcga catgagcctg atagagcgag atcacgttag tccagccgaa ttgcagatcg
     5881 cccgaagtca ccccctggac aatcactgcg ccgccggcca tcggtacggt ctctaattcg
     5941 atgccttcgg ctttaaaaaa gcctttttcc atcgccatga acatcggcgt ggtatcgacg
     6001 atcttcatgt aacccaactt ccccttgacc tgcgcccagg cgaaatccgg cgccagagac
     6061 aaaccggcaa taccaacggt gattttggta aggaagttac gacgtgagac tttcatgcag
     6121 cggctccttg tcgacatggt aagatcggtg acagcacatc gatgaattct tattacaacc
     6181 gcaaaaataa ttaaaggagc tggatcgacc cgacagtcac gttagagact tgggcagctc
     6241 actcgaggct cagacgattg gcgcgaaagg cgagctttca gccgccgctt gagatcatcg
     6301 acaattcccg attcgcccgc ggcgatgtcg acgagttcat ccgggtcgcg ttgcaaatcg
     6361 tagagtaacg cttcagggct ctggcaagga gcgatcaact tgtatttatc ccaccatatg
     6421 gcgattttag tcggcaaatg atgaaccacg ccatcgtagg aatatttgta gttgattgct
     6481 atggtttccg cctttacggg tgaagcgcca aacttgagcg gctgcccttc catccaatcc
     6541 ggcctgggaa tccccacagc atcaagaatc gtcggtgcga gatcgagaga ctgggtcagg
     6601 ccgcttaccc gctcgccatt tttctgtcca gggtagcgaa ttagcagcgg cgcccaagta
     6661 gaactttcgt agagttcatc cccatgcaaa aaaaagccgc gttcaaatga ttctccgtga
     6721 tcactggtta gaatcaccat agagttttcg actttggacg tttcttctaa aaattttccg
     6781 agttcggcgt ccaatgtttt aatagttctc tcataggagt cgcgataagt gtcgaccagc
     6841 ggctgaagct cggagcggta tggcgcggaa actgagagct gcgagcgagc tacattgccc
     6901 ccgggaactc gccgcgagcc ttcacgaatc tgataaggcc aatgaggttc ctggatatga
     6961 acaaacaaaa aaaacggcgg cgctaagcgc cccatcatat gcttcacggc cgcaaccgtg
     7021 tcctcgacgt tgccatcgag tgaagcgtgt ttcggaaaag ggagaaaagg taacattcgc
     7081 gaaatatcct gatacatccc tccactaaaa cgggtcggat agactccgag ccgctgcaac
     7141 cacgagaaca agtgaaatcc ataggccatg ttttcagtga ggtgcaatcc cactctcaag
     7201 ccctgcaagg catcagaggc atcgccatta ctcgtcactg caccgatcga gtaaccgtga
     7261 acgcttaaa
//
LOCUS       DATLCH010000241         4241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2889842, whole genome shotgun sequence.
ACCESSION   DATLCH010000241 DATLCH010000000
VERSION     DATLCH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2889842"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..2988
                     /locus_tag="VMZ02_07125"
     CDS             112..2988
                     /locus_tag="VMZ02_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HUR71740.1"
                     /translation="MADLPKGSTITLFDRKGTILTHFPNSHGSVGRTVPSIANPAATA
                     GSVNKIANGVSLDGQERLFGFRSVGEVSQDPWAVIQVGLLKEVALADAEWYFKRNLVA
                     LSLAGVLALLIAWYVGDNLFVRQLKSLARTTAMIGAGNFSARTGLSRRNNELDRFARS
                     LDSMAELLEQRDRETRIAKERSRRQLERINALHEIDMAISSTLDVRAVLRLLLEKVEL
                     VLPGAAATIRLFDQRMGLLKPLICHNVDEIAWLAESPSDLTGLAKIVLENRISLTVAN
                     VQSDPRATEGELDRKHGFVSYLGVPLVVARELLGVIGFYTREEHAFNDDEIEYLTALA
                     GQSAVAIHNARLFEETRRREREASALHAIAASASQSLDLDVILSEAVAKISANFAFDS
                     SHFLLFNPETSLLELRATYLGTGTSPLDDALWRDQKSFMKDIAESGEVISIEDVLTDP
                     RCHEVWVLNSQNLNDLRFLTIFPVKTKLKRWGLVLFAGREPRRLSDDEIRLLESMTHQ
                     IGVAVENAMLYEQTVAKAKELATLYSFTALASQSLDLQLLLRQTTEKLLEMFRFDAVR
                     VFLREGNSDAVVLVTKLGFPTGFSMRDRYQIGEGRIGGVFNSGNPMFVPDMNSDSSYW
                     SAAQNDEMLTLGFRSSFLMPINAAGKCLGVINLLCRRLYQFSPSDVQLIEAAASHLGT
                     AVGNANLYFQIQQKSHELEKANKAKDEFLGVISHELRTPLSIIKGYADILKHRVFGDV
                     TGEQVAALEKIMIQASALTDMINDVLQVTAIGAMKTRLVCADLDLVSFLSELRESYQF
                     ANDASIQVSWIFNADLPAVRTDAEKLKAILQNLINNSLKFTETGSVKIAVKQLALGDG
                     IELTVRDTGIGIPADKIDTIFGMFQQADNSSTRGYGGVGLGLYIVKSYTELLGGHVSV
                     WSEIGEGTCFTVTLPLNLDTKCVASGSAAVPSQAFPEVEPAAIT"
     gene            complement(3040..3933)
                     /locus_tag="VMZ02_07130"
     CDS             complement(3040..3933)
                     /locus_tag="VMZ02_07130"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71741.1"
                     /translation="MPRTKIRIATAAPSLSYFPIYAAVQKGFFARRGFDVEMIQMAPS
                     LTAAALLNRSVDYTIIPSTIATAAARGAPAKVIHFASVKLQHTLVVRADVNSVSDLAG
                     KKIAGSGFGNLVAYEIQYLIERYKLGPKTTIVNAPSSIDRLIAVQKGLAEAAIIAAPA
                     DIKGEEMGLKRLIQMGSLLQIPQAGLAATDDKLKTNRGEVIEVLKAAIDGLEYTANQR
                     DDAIALIGKWMALTPPQALKAYESVKETYSRDGVPTPEQSKAYIAMLAATAGLNADLP
                     AATIFDFSLSTAAAKDLLSKR"
BASE COUNT          965 a         1079 c         1184 g         1013 t
ORIGIN      
        1 gaacgttccg gcagagccgg cttgaatgtt ggttttccag ttcgtcagaa cgatagcgtg
       61 ggccttgcat atgttgagtt ggacttggaa tggctcgctc agattgcaaa aatggcagac
      121 ttgcctaaag gctcgacgat caccctattt gaccggaaag gcaccatcct aacgcacttt
      181 ccaaatagcc atggctcggt gggacgcaca gtgcccagca ttgcaaatcc cgctgcgact
      241 gccggcagcg ttaataaaat cgcgaatgga gtgtcgctcg atgggcagga gcggcttttc
      301 ggctttcggt cagtaggtga agtgtcgcaa gatccctggg ccgtgattca agtcggattg
      361 ctcaaggaag tcgccctcgc cgatgccgaa tggtacttca agcgtaatct cgtggcatta
      421 tcattggccg gggtattggc tttactgatc gcctggtatg tcggggataa tctattcgtg
      481 cgccagctca agtccctggc cagaaccaca gccatgatcg gtgcgggaaa ctttagcgct
      541 cgtaccggac tgagtcggcg caacaatgaa ttggaccgct ttgcgcgctc gctggactcc
      601 atggccgaat tgctcgagca gcgcgatcgt gaaaccagaa tcgccaagga gcggtcgcgc
      661 cgccaactcg aacgaatcaa tgccttgcac gaaatcgaca tggcgatttc atccactctt
      721 gatgtgcgcg ccgttctgcg tttgctgttg gaaaaggtcg agctggtgct tcccggcgcc
      781 gcggcgacaa ttcgattgtt tgaccaacga atggggcttc tgaagccgct catttgccac
      841 aatgtcgatg aaatagcctg gcttgctgaa tcgccgtcgg acctgaccgg cttggccaaa
      901 atcgttctgg agaatcgtat ttcgttaaca gttgccaatg tccaatccga tccgcgggca
      961 acggaaggtg aactcgacag aaaacacgga tttgtctcat acctgggagt tcccctggtt
     1021 gttgctcgcg aattgctcgg cgtaatcggg ttctataccc gggaggaaca cgccttcaac
     1081 gacgatgaga tcgaatacct tacggctctc gctggacagt ccgcggtggc aattcataat
     1141 gcccgattgt ttgaagaaac gcgccgacgc gaacgcgaag catccgcatt acatgccatc
     1201 gcggcctcag cttcgcagtc gttggatctc gacgtgatcc tcagcgaggc ggttgccaaa
     1261 atttcggcca atttcgcgtt cgactcaagc cattttctac ttttcaatcc agagacctct
     1321 ctgttggagt tgcgagcgac atatttgggg acgggaacaa gtccgctcga tgacgcgctt
     1381 tggcgagacc agaaatcgtt catgaaggac atcgccgaat cgggggaagt gatatccatt
     1441 gaggatgtgc tgaccgatcc acgttgtcac gaggtgtggg tactcaatag tcagaattta
     1501 aacgatctgc gttttctgac cattttcccg gtgaagacaa aactcaagcg ctggggtctg
     1561 gtcctctttg ccggacgcga gccgaggcgg ctgagcgacg atgagatccg gttgctcgag
     1621 tcgatgaccc atcagatcgg cgtcgccgta gagaacgcga tgctgtacga acagaccgtc
     1681 gccaaagcca aggagctggc gacgctgtat tcgttcaccg ctctcgctag ccagtcgctg
     1741 gaccttcagt tgctgcttcg gcagacaacg gaaaaacttc tcgaaatgtt taggttcgat
     1801 gcagttcgag tatttctgcg cgaaggcaac agcgacgcag tcgtgctagt tacaaaattg
     1861 ggctttccga caggattctc gatgcgcgat cgctatcaga ttggcgaggg ccgcattggc
     1921 ggggttttta acagcggtaa cccgatgttt gttccggata tgaatagcga ctcttcctat
     1981 tggagcgcgg cgcagaacga tgagatgctc acactaggat tccgcagttc ttttttgatg
     2041 ccgatcaatg cggcgggaaa atgtttggga gtcataaacc ttctttgtcg ccggctttat
     2101 caattttcgc ctagcgatgt tcagctgatc gaagcagctg cctctcatct ggggactgcg
     2161 gtcggcaatg ccaaccttta tttccaaatt cagcaaaaat cacacgaact cgagaaggct
     2221 aacaaagcca aggacgagtt tctcggtgtg atttcccacg aattgcgtac gccgctcagt
     2281 atcatcaagg gctatgccga catactcaaa cacagggttt tcggcgacgt gactggcgaa
     2341 caggtggcgg cgttagaaaa aattatgatt caagcaagcg cgctgaccga tatgatcaac
     2401 gacgtgttgc aggtgacagc gatcggagca atgaagacac gattggtttg tgcggatctc
     2461 gatctggttt catttctgag cgagcttcgc gagagttatc aatttgccaa cgatgcgtcc
     2521 attcaggtca gctggatctt caacgcggat ttgccggccg ttcgtacaga cgccgagaaa
     2581 ttgaaggcga tactacagaa cctaattaat aattctctca agtttactga gacaggatcg
     2641 gttaaaatcg cggtgaaaca actcgctttg ggcgacggca ttgaattgac ggtgagggat
     2701 accggcatcg gcattcccgc cgataagatt gacacaatct tcggcatgtt tcagcaagcc
     2761 gataactctt ctactcgcgg atatggcggc gtcggactcg gattatatat cgtaaagagt
     2821 tataccgaac tgcttggcgg tcatgtctcg gtatggagcg aaatcggcga gggcacatgt
     2881 ttcaccgtta ctctgccgct caatctcgat accaaatgcg ttgcctcggg ttctgccgcg
     2941 gttccgagcc aggcgtttcc tgaggtcgag cccgctgcga taacttagaa gctcatttct
     3001 cacgatcggg cccaagggag tccttcccgt caccgctaat catcgtttgc tcaacaaatc
     3061 tttggcggcc gccgtggaca gcgaaaaatc gaagatcgtc gctgccggta gatcggcgtt
     3121 tagtcccgcg gtggcagcga gcatggcgat gtaggctttg ctctgttccg gcgtcggcac
     3181 accgtctcgc gagtaggtct ctttgaccga ctcgtaggcc ttcaacgcct ggggcggagt
     3241 gagtgccatc catttgccga tcaaagcaat ggcgtcgtcc cgctgatttg cggtgtattc
     3301 cagaccatcg atcgccgcct tgagtacttc gattacttcg ccgcgattgg tttttaactt
     3361 gtcatcggta gcggcaagac cggcctgggg aatctgcagc aggctgccca tttgaatcaa
     3421 gcgtttgagc cccatttctt cgcccttgat atctgccgga gcggcgatga tggcggcttc
     3481 ggcgaggcct ttctgcacag cgatcaagcg gtcgatggac gagggcgcat ttacgatggt
     3541 ggttttggga cctagtttgt agcgctcgat gagatattga atttcatacg cgaccagatt
     3601 gccgaagccg ctaccggcga tttttttacc cgccaaatca ctcaccgaat tgacatcggc
     3661 gcgcaccacc aaggtgtgtt gcagtttcac cgaggcgaaa tggataactt tcgccggcgc
     3721 gccacgggcg gcggcggtgg cgatggtgct gggaatgatc gtgtaatcga cggaccggtt
     3781 gagcaacgcc gcggcggtca aactaggcgc catctgaatc atctctacat cgaagccgcg
     3841 cctcgcaaag aagccctttt gcaccgcggc gtaaatcgga aaataactta gcgagggagc
     3901 ggcagtggct atgcggattt ttgttcgtgg cataatatcc gctgcaagga gcggtaatgt
     3961 gattggcgga gcggtcaaga tcaattgagt gaagagacag catagaatgg ccaatcgttt
     4021 catatgatct ccagaggtct ttctaaaaag tcgattgttt gatttctttc ggctaactgt
     4081 cgtcgtaaaa cgattcgaac aagaccctgt tcgcgcggcg cagtttcgcg gcaaagaatc
     4141 gcgacatagc gttccactaa tcagaggaac gatgaacgcg gtaaatcttt gtggacccag
     4201 cagatttggc caaaaaaagt tcgagcaaat atactcggca a
//
LOCUS       DATLCH010000242         5212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9595803, whole genome shotgun sequence.
ACCESSION   DATLCH010000242 DATLCH010000000
VERSION     DATLCH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9595803"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VMZ02_07135"
     CDS             <1..207
                     /locus_tag="VMZ02_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR71742.1"
                     /translation="DYFRRMEGVDVDNLYSLVIERIERPLIELTLKKTKGNQIKAAEI
                     LGINRNTLRKKISDLGIELKKDPG"
     gene            212..829
                     /gene="thiE"
                     /locus_tag="VMZ02_07140"
     CDS             212..829
                     /gene="thiE"
                     /locus_tag="VMZ02_07140"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HUR71743.1"
                     /translation="MAVSLPALYAILDPEQIGQRAVEDVLSELLHAGVKMLQLRAKAL
                     GGRDFLALAKLARSQTETYNCRLIVNDRVDVAMLCNADGVHLGQEDLPLEVGRRLLGN
                     QIIGISTHNIEQARQAQDGGADYIGFGPMFGSQTKDTGYSARGIDMLTEIRATVTIPI
                     VAIGGINEQNVSQVWRAGADSAAIISDILASRDITAKIDRILSQA"
     gene            951..1718
                     /locus_tag="VMZ02_07145"
     CDS             951..1718
                     /locus_tag="VMZ02_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HUR71744.1"
                     /translation="MNEPIPRRVPKKTLKGQWNLVRFRFQENFYALRGRKIVHLLHIG
                     KTGGTAVKHALHTDLVTSQYLVRRHGHQTRLDQIPTGQSVVFFLRDPQSRFISAFYSR
                     LRQGRPRYNNPWKAHEIVIFNRFPTPNRLALGLSSDDDEVRGQAEQAMQSISHVRDSY
                     FRWFGSEEYFLSRLADVFFIGFQETLNDDFEELKIKLGLPNSLTLPNDDVLAHRSPRE
                     LDRTLDEEAVRNLRRWYAADFKFVELCRSQAQQINAR"
     gene            1797..2261
                     /locus_tag="VMZ02_07150"
     CDS             1797..2261
                     /locus_tag="VMZ02_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HUR71745.1"
                     /translation="MSFSTTPDHRYFEDYMPGAVHEFGAVGVDEQEVLDFGRRFVPLA
                     YHVDKEAAKKSIYGGVIASGWHTAALMMRVYTENYLSSVANLGSPGGDELRWDKPVFP
                     GDVLSVRATVLETRRSESRPDRGIVRTFIEVLNQKRDVVMTMKMVNFVRCRI"
     gene            complement(2304..3167)
                     /locus_tag="VMZ02_07155"
     CDS             complement(2304..3167)
                     /locus_tag="VMZ02_07155"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HUR71746.1"
                     /translation="MQLRLINNLSLLAVCLFTASLTIIAAVHAANAPALLLANELGPQ
                     IDPAKYLVSEKFDGVRALWDGRELRFRSGRSVNAPTWFIAKLPAQQLDGELWLGRGQF
                     ENLSAIVRKTEAQDDEWRQIKYMIFELPNAPGTFAARAQRVREIVAETHWPQLVAVEQ
                     FRVADRVALKKRLEEVVRGGGEGLMLHRADAPYITGRSDALLKLKPLQDTEAVVIEHI
                     PGKGKYVGMMGALRVRTPEGKVFLIGTGFIDEVRKKPPPVGTTITYTYRGMTSTGVPR
                     FASYLRVRQEF"
     gene            complement(3235..4614)
                     /gene="dnaB"
                     /locus_tag="VMZ02_07160"
     CDS             complement(3235..4614)
                     /gene="dnaB"
                     /locus_tag="VMZ02_07160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HUR71747.1"
                     /translation="MAALEDNLRKIPPQNLDAESSVLGGILIENEAVNVVLETLRPED
                     FYRESHRRIMRAMIEITDRSEPVDLITLSDYLKSKNELESVGGTAYLASLADFVPTAA
                     NIAYYVRIVHEKAILRNLISTATEIATRGYDEQGNVDEFLDSAEKIIFDISEKKIKQS
                     FVAVGDMITDTLKTIDKLYQRKEMVTGVPTGYEDLDKITAGLQPSDLIIVAGRPGMGK
                     TAFALNIAANAAYAGIGAAVFSLEMGKEQLVLRMLCSEARVNSSKVRSGYLGERDFPQ
                     LAKAAGKLHEAPIYIDDTPAISVLELRAKARRLLRDRSKRVGLIVVDYLQLMRGMGQA
                     NNREQEISEISRSLKALAKELRVPVIALSQLNRRVEDRSERRPQMSDLRESGAIEQDA
                     DVIMFIYRDEVYNKNNDDVKGKAEVIIAKQRNGPTGSIDLTFIGEFTRFENYSERDEY
                     GGRDEMDEA"
     gene            complement(4908..>5212)
                     /gene="queF"
                     /locus_tag="VMZ02_07165"
     CDS             complement(4908..>5212)
                     /gene="queF"
                     /locus_tag="VMZ02_07165"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HUR71748.1"
                     /translation="RDFEINMECPEFTCICPKTGQPDFATIHITYVPDKLCIELKSLK
                     LYLWSYRNEGTFHEAVTNKILDDLVSACQPRKMTVLGDFNVRGGIHTTVTATYQQS"
BASE COUNT         1215 a         1429 c         1388 g         1180 t
ORIGIN      
        1 gactattttc gacgcatgga aggcgtcgac gtcgacaatc tttattcact ggtgatcgaa
       61 agaatcgagc ggccactgat cgaactcaca ctcaagaaaa ccaaaggcaa ccaaatcaag
      121 gcggcggaga ttctcggaat caaccgcaac accttgcgca agaagatttc cgatctcggt
      181 attgagctca agaaagaccc tgggtagccg aatggccgtt tctctgccgg cgctctacgc
      241 cattctggat ccggaacaga tcggacagcg cgctgtcgaa gacgtattga gcgagctgtt
      301 acacgccggc gtcaagatgc ttcagctgcg cgctaaagca ctcggcggga gagattttct
      361 ggcattggct aaattggctc gtagccaaac ggaaacctac aactgtcgct taatcgtcaa
      421 cgatcgcgtc gatgttgcga tgctgtgtaa cgccgacggc gttcacttag gccaggaaga
      481 cctgccgctc gaagtcggtc gcagactgct gggtaaccag attatcggta tctccacaca
      541 caacatcgag caagcgcgtc aagcgcaaga cggcggcgcc gattacatcg gcttcggccc
      601 catgttcggt agtcaaacca aagacaccgg ttacagcgcc cgcgggatcg acatgctcac
      661 ggaaatccgc gcgacggtta cgattccaat agtcgcgatt ggtggcatta acgagcaaaa
      721 cgtcagtcaa gtttggcgcg ccggggcgga ctcagccgcg atcatcagcg atattttggc
      781 aagtcgagat attacggcga agattgacag aattctgagt caggcttgaa cccaaatcgc
      841 atagatttga ccagaagggt ttgcaaactc ttgacagaaa tcggcgtgct gtcccatata
      901 gtagcaagaa tattgcggca gtcggcgaat aatatgcagc taaggacatc atgaacgaac
      961 cgatacctcg ccgggttcct aaaaagaccc tcaagggaca gtggaactta gtccggtttc
     1021 gctttcagga aaacttctac gccctgcgcg gtcgcaaaat cgtgcacctt cttcacatcg
     1081 gcaaaaccgg cggtaccgcg gtgaaacatg ctctacatac ggatttagtt acgtctcaat
     1141 atctcgttcg gcggcacggc catcagactc gactagatca aattcctacg gggcaaagtg
     1201 tcgtattttt tttgcgcgat ccgcagtcgc ggtttatcag cgccttctat agccgacttc
     1261 gccaaggtcg gccacgctac aacaatcctt ggaaagccca cgagatagtc atatttaata
     1321 gatttcctac tcctaaccgt ctagcccttg ggctttccag cgacgatgac gaggtgcgag
     1381 gtcaggcgga gcaagctatg caatcgatct cccatgtgcg tgattcctac tttcgttggt
     1441 ttggcagcga ggagtatttt ttatcccgtt tggcggatgt atttttcatt ggctttcagg
     1501 aaactctcaa cgacgacttt gaagaactga aaattaaact cggattaccg aatagcctca
     1561 cgctcccaaa cgacgatgtt ctcgcgcacc gctctccccg ggaacttgac cgaaccctcg
     1621 acgaggaagc ggtccgcaat ctcagacgtt ggtatgctgc agacttcaag ttcgtcgaac
     1681 tttgccggtc acaggcacaa caaataaatg ccagataaca ttccctggaa ccatcacgtc
     1741 tcgaagctaa cgccaccgat caattgccgg tatttaacgc ccgagcgagg agcaccttga
     1801 gtttttccac cactccagac catcgctatt tcgaagacta catgcccggc gcggtgcacg
     1861 agttcggggc ggtcggcgtc gacgagcagg aagttctcga tttcggcaga cgctttgtcc
     1921 cgctcgccta ccacgtcgat aaagaagcgg cgaaaaaaag tatttatggc ggcgtcattg
     1981 ccagcggctg gcacaccgct gcgctgatga tgcgcgtcta cacggaaaat tatttatcga
     2041 gcgtggccaa tctcggctca cccggcggcg acgaattgcg ctgggacaaa ccggtttttc
     2101 ccggtgacgt tctctccgta cgagcgaccg tcttggaaac ccgccggtcc gaatctcgcc
     2161 ccgaccgagg catcgtccgc acgttcatcg aagtgctcaa tcagaaacgc gacgtggtga
     2221 tgacgatgaa gatggtgaac tttgtgcgct gccggatctg agcgtcgccg acaaccggga
     2281 ggcctgggcg ctgaccgact tcgtcaaaac tcctgacgca cacgtaaata actggcaaaa
     2341 cgtggcacgc cagtgctggt catcccgcga taggtatacg tgatagtggt tcccaccggc
     2401 ggcggcttct ttctgacttc atcgataaag ccggtgccga tcaaaaatac tttgccctcc
     2461 ggagtacgaa ctcggagggc gcccatcata ccgacatatt tacctttgcc gggaatatgc
     2521 tcgatcacca ccgcttcggt gtcttgcaat ggcttcaatt tgagcagcgc gtcgctgcga
     2581 ccggttatgt agggtgcatc ggcgcgatgc agcatcagtc cttcgccacc gccgcgcacc
     2641 acctcctcca atctcttctt caacgcgacg cgatcggcga cgcgaaattg ttcgacggcg
     2701 accaactgcg gccagtgagt ctcggcaaca atttctcgaa cgcgctgagc gcgcgcggca
     2761 aacgttccag gcgcgttggg cagttcgaag atcatgtact tgatctggcg ccattcgtcg
     2821 tcttgcgctt cggtcttgcg cactatggcg gaaagattct cgaactggcc gcgcccgagc
     2881 cagagttcgc cgtcaagctg ttgagccggc aacttggcga taaaccaggt cggcgcattg
     2941 accgagcggc cgctgcggaa gcgcaactcc ctcccgtccc atagagcgcg cacgccatcg
     3001 aacttttcgc tgacgagata cttggccgga tcgatttgag gtccgagctc attggcaagg
     3061 agtaacgctg gcgcattcgc ggcgtgaacg gcggcgatga tcgtgagtga tgccgtgaaa
     3121 aggcaaaccg ccaacaatga gagattgtta atcaacctga gctgcatcaa taatgcacgt
     3181 gactctgcat aggaaatctg aagttgcaat gcggatggcg tttttaccat gccgttacgc
     3241 ctcatccatc tcatcccgcc caccatactc gtcgcgctcg ctgtagttct cgaagcgcgt
     3301 aaattctccg atgaatgtta gatctatcga acctgtgggg ccattgcgct gtttggctat
     3361 gatcacttcc gctttgcctt ttacgtcgtc gttgttcttg ttgtagacct cgtctcggta
     3421 aatgaacatg ataacgtcgg cgtcctgctc gatggcccca gactctctca agtcggacat
     3481 ctgcgggcgc cgctcggagc ggtcttcgac gcggcggttg agttgtgata gcgcgatcac
     3541 tggaacacgc agttctttgg cgagagcttt gagcgagcga gaaatttccg aaatctcctg
     3601 ctcgcggttg ttggcctggc ccattccgcg catgagctgc aaataatcga ccacgataag
     3661 tccgactcgc ttcgagcgat cacgcaacaa ccggcgcgct ttggcgcgca actcaagcac
     3721 tgaaatagcc ggggtgtcgt cgatatagat cggcgcctcg tgcaatttgc ccgcggcctt
     3781 ggccaattgc gggaagtcgc gctcgcccag atagcccgag cgcacttttg agctattgac
     3841 ccgcgcttcg gaacagagca tgcgcaagac taactgctcc ttgcccattt caagcgagaa
     3901 taccgcggcg ccgatcccgg catacgcggc gttggcggcg atgttgagcg caaacgcggt
     3961 ctttcccata ccgggccgtc cggcaacgat aatcaagtct gaaggttgca agccggcggt
     4021 gatcttgtcc aggtcttcgt agccggtggg cacaccggtg accatttcct tccgctgata
     4081 aagcttatcg atggtcttga gcgtgtcggt gatcatgtct ccgaccgcaa cgaaggattg
     4141 ttttattttc ttttccgaga tgtcgaagat gattttttcc gcactgtcga gaaactcatc
     4201 gacattgcct tgctcatcgt aaccgcgggt ggcgatctcg gttgccgtgg aaattaaatt
     4261 gcgcagaatc gctttttcat gaacgatgcg cacatagtac gcaatgttgg ccgccgtcgg
     4321 cacgaaatcg gccaacgacg ccaaataggc ggtgccgccc accgactcga gctcgttttt
     4381 acttttgaga tagtcgctca aagtaatcaa atccaccggc tcgctgcgat cggtgatctc
     4441 gatcatcgcg cgcatgatgc ggcgatgcga ctcacggtaa aaatcctcgg ggcgcaacgt
     4501 ctcgagcacc acgttgaccg cctcgttctc gattaaaata ccgcccaaca ccgaggactc
     4561 cgcgtcaagg ttttgcggcg gaatcttgcg cagattatct tccaaggcag ccatggattg
     4621 tttctgaacc gcgagaacga ataagaccga tttaccagag tagaaaaaat tttgttccag
     4681 gaatctaagt ttataaaaac caaaatgcaa gaaaaatttt gccgaatcgg caaccgcgct
     4741 tccgaaaaaa ccgcgcttag attttgtgca attcggtcaa aaaaaaaatt tagcgacgca
     4801 gcggggcgct agtgaaaaac ctgatcacag tttgtgcaca acttactaac agtctggcgg
     4861 gaaacagaaa ttttttgccc agagcagccg acggcaaaag ccgccggcta gctttgctgg
     4921 taggttgccg tcacggtagt gtggatgccg ccgcgcacat tgaaatcgcc gagcacggtc
     4981 attttgcgcg gctgacaggc gctaaccagg tcgtcgagaa ttttattggt gacagcttcg
     5041 tgaaatgtgc cttcgttgcg gtaggaccat agatagagct tgagtgattt tagttcgata
     5101 caaagcttat ccgggacata ggtgatgtga atcgtggcaa aatcgggctg gccggtcttc
     5161 gggcagatac aggtgaattc ggggcactcc atattgattt caaagtcgcg ct
//
LOCUS       DATLCH010000243         6895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5863948, whole genome shotgun sequence.
ACCESSION   DATLCH010000243 DATLCH010000000
VERSION     DATLCH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5863948"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VMZ02_07170"
     CDS             complement(<1..325)
                     /locus_tag="VMZ02_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71749.1"
                     /translation="MKRLTAFILWGIIAQGCEPAVTQFPPGSVQNTGGSATPQQAQID
                     KDRRWSHAVESAQKAAFLGGIFGGPFGGPASVGMGAIGFIYGMFTAEAKIAEEKAEAQ
                     VRYQKE"
     gene            complement(395..1558)
                     /locus_tag="VMZ02_07175"
     CDS             complement(395..1558)
                     /locus_tag="VMZ02_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71750.1"
                     /translation="MAATMILFLITVLLLQSSSPTYAQHPIASFFYDERGSVVRQEQD
                     TNGDGKMDRWTYYNAQGRVERVEQDVNFDGKPDTFVVYEAGRLVRQEIASKNDGQIDT
                     WFFYNSNGEIERRGQDPGRSGKPTVWVYYQNGQAVRSEEDTKSSGKTDRTVHLRDGQI
                     NRVDEDTNGDGKPDSFSYYVNGQLARKEFDRKFNGRIDLWGYYQSGRLVRQEEDLRGD
                     GKISRIVHFDGEQVLRREEDTGKRGRMDMIESFVQGKIVKRLRDSKASGKWDTVYYFE
                     NNELTREERDTNGDGFFDLRILYEKGQIRAQDADTNGDRRVDVWVNFINGERVEQLED
                     QNGQGKITARYVFKGGQVVGQEAVSDGDAPRVSPPFGSVTEEYQAMAIAVPPA"
     gene            1788..3536
                     /locus_tag="VMZ02_07180"
     CDS             1788..3536
                     /locus_tag="VMZ02_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUR71751.1"
                     /translation="MPPTSPKISDQARLPSRQDPSDRWFPFFLFAFVLLLLFYRLGDA
                     ALFEPDEGRNAEKAREILLLNDWITPHENFHAVLDKPMFFYWLIALSYKLFGVSEWAA
                     RLPSALAAFSCVLVVYLFVRRWWGEWEARWSVLALITTAGVFVFSRIVIFDMTLTAGI
                     TLALSAFYQTARAPESKASWGVCAILYLALAAATLTKGLVGLVVPGMVFFFYLLLSKS
                     WSVLRRIRLIPGTALFLLVVAPWYILAEIHNPGYLRYYLWEEHFGRFATTKFDRFSPW
                     YFYLYVVPLGLLPWTFLLPAAIKNYWRRKLDDRTLWLILWAGLPILFFSFSKAKLPHY
                     ILPSLPALAILVGVALPRALCESQDRCRFGFAAGWLVLSSAFLYSAAGVIRPEILPEI
                     ARGRFDSIVTLFWISSAISLVLAYLAGKRRIWTKVSPKYIFFAQAAGMLIFVITLSEM
                     MVLISPARSAKEIARKALPFLSPTTQVVSYDTYAEALPFYLKAEKPLWVVTHNHKKRT
                     FLGNFYAITGQPEPITRWGKALLDFDEFSAIWQSSDIPLLILVKEKNLRGLERRVGTA
                     LNRVAEDDEYLILKRP"
     gene            3615..4439
                     /locus_tag="VMZ02_07185"
     CDS             3615..4439
                     /locus_tag="VMZ02_07185"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HUR71752.1"
                     /translation="MNNFVIAAVLGVVEGLTEFLPVSSTGHLIIAGHLLGFVGEKAAS
                     FEVAIQLGAILSVVWLYWRRFVALLPGMAQWPLAAESTLIGWPGLQRIALATVPALTV
                     GYLARHVIKAQLFNPEAVTLALATGGVAILLVERLTARRYATPVDAVTLGQALGVGLF
                     QILALWPGTSRAAATIIGGMILGLERKAAAEFSFLIAVPVLFVASGYELLKMRDQIVA
                     DDAISLTIGFVISFVVALLAIKVFVSYLSRGKLAPFAWYRIVVAPIFYFLTRGAAL"
     gene            complement(4486..5217)
                     /locus_tag="VMZ02_07190"
     CDS             complement(4486..5217)
                     /locus_tag="VMZ02_07190"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUR71753.1"
                     /translation="MSDQRRRLAIRGAIFISLILLLFFFSRYIPRPFSAFAPPGAELD
                     DFEGIYSSSDKLGKFLQTLGPYSPAVFVLLQILQVIAAPFPGELTGVAGGFVYGETIG
                     FILSTIGLSIGSWVAFELASILGRPFVERFIKRDVLEKFNFLTTKTGVVICFVLFLFP
                     GFPKDYLCYVLGLSRMSVTTFLLVSVIGRIPGTYLLTMQGATIRNQQYHSFAIFMAVT
                     LVAIVVGYIYRNQIFNWIKHRHDHD"
     gene            5357..6889
                     /locus_tag="VMZ02_07195"
     CDS             5357..6889
                     /locus_tag="VMZ02_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HUR71754.1"
                     /translation="MFADLKCGTLAELESGRSWSSQEVAVRVASRADVMRSHGLGRGD
                     RLFILYGNKLEFFVDLLAAWHAGAGVVPVDNRLTPFEVENLARAVTPKIALVDATVPS
                     GLGDALAAMGVKVLSSAEPTETIPAVLAHSLGCAARLDDEALILFTSGTTGVPKGVVH
                     THRSLRARWFSLRQSLGIECFQRTLCLLPTHFGHGLICNSLFPWLFGQDLVIASPFSP
                     YLLLRLGPIIDEHNITFMSSVPSMWGLSLKGSKPPLGKSLRRIHCGSAPLSAHQWRQI
                     REWSGIRAVYNAYGITETASWVAGTSIENFEPEDGLVGEPWGAAIKILKSPERQAPFA
                     DANECKVSETGYVWINTPALMQGYFGQPELTDQVISGGWFATGDIGLVDDRGRFYLKG
                     RERDEINKGGAKVYPTDVDAVVERFEHAIAVCTFAIADPAYGQNVGMAVVLSRHDDAT
                     LRQLHAWLKEHLAEFKIPQRWYVVDELPLNSRGKVSRNTVQELCALLKPVDLVGLLRR
                     VS"
BASE COUNT         1459 a         1789 c         1874 g         1773 t
ORIGIN      
        1 attccttttg gtaacgcacc tgggcttccg ccttttcctc ggcgatcttc gcttcggcgg
       61 taaacatgcc gtaaataaaa ccgatcgcgc ccattccgac actagctggc ccgccgaacg
      121 ggccgccgaa gattccgccg agaaaggcgg ctttttgggc actctctacg gcgtgagacc
      181 agcgtcgatc tttatcgatt tgcgcttgtt gtggggtggc actgccaccg gtattttgca
      241 cggaaccggg aggaaactga gtaaccgcag gctcgcatcc ttgagcgatt atgccccaca
      301 gtataaatgc agtgagcctt ttcatgaaac tattccaacg tcagcaatcc tactgatttc
      361 gccacccctg agcaagtcag atatcgagta taagctacgc gggtggtacg gcaatagcca
      421 tagcctggta ctcttccgtt acactgccga acgggggcga gactcgcggt gcgtctccgt
      481 cgctcaccgc ctcctgaccg acgacctggc cgcctttgaa aacatagcgg gcggttatct
      541 taccttgacc gttttgatct tccagctgtt cgacgcgctc accattgata aaatttaccc
      601 agacatcgac gcgccggtcg ccattggtat cggcgtcctg agcgcgaatt tggccctttt
      661 cgtagagaat ccgcagatcg aaaaagccat cgccgttggt atcacgctcc tcgcgcgtca
      721 attcgttatt ttcaaaatag tacacggtgt cccatttacc gcttgctttg ctgtcgcgta
      781 gtcgcttgac aatttttccc tgaacgaaag actcgatcat gtccatgcgg ccgcgtttgc
      841 cagtatcttc ttcgcggcgc aagacctgct cgccgtcaaa atgcacgatg cggctaattt
      901 taccgtcgcc gcgcaagtct tcttcctgcc tgaccaagcg gccagactga tagtatcccc
      961 ataggtcaat tctgccgttg aatttacgat cgaactcctt gcgcgcgagc tggccattta
     1021 cataataaga aaaactgtca ggcttgccgt cgccattggt gtcttcatca actcggttga
     1081 tttggccgtc gcgcaaatgg accgttcgat cggttttgcc gctggacttg gtatcctcct
     1141 cgctgcgcac cgcctggccg ttctgataat aaacccaaac tgtcggctta cccgaacggc
     1201 cgggatcctg gccgcgccgc tcgatttccc cattcgagtt gtagaaaaac caggtgtcga
     1261 tctgaccatc gttcttactg gcgatctctt ggcgaaccag tcggcccgct tcatagacaa
     1321 cgaaggtatc gggcttgccg tcgaagttaa catcctgctc tacccgttcg acccggcctt
     1381 gcgcgttgta gtaggtccaa cgatccattt taccatcgcc attggtgtcc tgttcctggc
     1441 ggacgacgct gccgcgttcg tcgtaaaaaa aactggcgat agggtgctgg gcgtaagtgg
     1501 gtgacgatga ctgaagcagc aatactgtga tcagaaatag gatcatcgtg gcggccatgt
     1561 caaagtaccg cgttgccgga gactaacgat caggagggtc aagctacgcc aggggtttag
     1621 cgatgtcaat tgacgcatct tgctgcggag tcgtgcaaac tataactttt tgatcgggcc
     1681 atatctgcga tgccgtagcg cgctcgaggg ccttaccttg ataatttagc gacgtcaggt
     1741 aaattcaacc aaagctaaac tgaccatcga actcccccgg tctttttgtg ccaccaacat
     1801 cacctaaaat ttccgatcag gctcgcctgc ctagccggca agatccgtcg gatcgctggt
     1861 ttccgttctt cttgttcgcg tttgtattgt tgctactttt ttatcggctc ggcgatgccg
     1921 ctctgttcga accggacgaa ggtcggaatg ccgaaaaagc acgtgagatt ctgttgttga
     1981 acgactggat cacgccccac gaaaatttcc acgcggtact cgataagcca atgttttttt
     2041 attggttgat cgcgctgtca tataaattgt tcggcgtgtc cgaatgggca gccagactac
     2101 cgtcggcgct tgccgccttc agctgtgtcc tcgtagtcta cttatttgtg cgccgctggt
     2161 ggggtgagtg ggaagctcgt tggagcgtgt tggcactgat caccaccgcg ggagtttttg
     2221 tcttttcgcg catcgtaatt ttcgacatga ctttaaccgc tgggatcact ttggcgttgt
     2281 cggcttttta ccaaaccgcc cgcgcacccg agtccaaggc gagttggggc gtgtgcgcaa
     2341 ttttatatct cgctctggcc gccgcaacgc tcaccaaagg gcttgtcggt ctggtcgtgc
     2401 caggcatggt ctttttcttt tatttactgc tgagcaaaag ctggtccgtc ctccggagga
     2461 ttcgcttgat acctggaaca gcgttgttcc tgttggtcgt cgcaccatgg tatatcctag
     2521 ccgaaataca taatcccggt tatctacgct actatctttg ggaagagcat tttggtcgat
     2581 ttgctacgac gaaatttgac cgtttcagtc cttggtactt ttatctttac gtagtgccgc
     2641 ttggtttgct tccttggacg tttttgttgc cggctgcaat taagaattat tggcgccgca
     2701 aactcgacga tcgaacgctc tggttgattc tgtgggcggg tttgccgatt ttgtttttct
     2761 cgttttccaa ggccaaattg ccgcattaca ttttgccgag tttaccggca ttagcgatcc
     2821 tagtcggcgt cgcgctgccg cgggcattgt gcgaatcgca agatcgctgc cgcttcggct
     2881 tcgccgcagg ttggctagtt ttgagttctg cgttcctcta ttcggccgcc ggcgtgattc
     2941 gtccggagat attgccggag atcgcgcgag gccgcttcga tagtattgtc accttgtttt
     3001 ggatcagttc ggctatctcg ctcgttttag cctatctcgc cggcaaacgc aggatctgga
     3061 cgaaggtcag ccctaaatat atttttttcg ctcaggctgc aggcatgttg atttttgtga
     3121 ttacgctcag cgaaatgatg gtgctgatat ctccggcgag atcggccaag gaaattgccc
     3181 gcaaggcgct gccgttccta tctccaacga cgcaggtagt ttcgtatgac acctacgcgg
     3241 aagcgctgcc attctatcta aaagccgaga agccgctctg ggtggtaact cacaatcata
     3301 aaaagcgtac atttctcggc aatttttacg caattacggg gcagcccgaa ccaatcactc
     3361 gctggggtaa agctttgtta gactttgacg agtttagcgc tatctggcaa agctccgata
     3421 ttccgttgct gattcttgtg aaagaaaaaa atctgcgcgg attggaacgg cgcgtcggca
     3481 cggctctcaa cagagtcgcg gaggacgacg agtatctgat ccttaaaaga ccctaataac
     3541 tattacgaga tcttttctcg tcgcgtgcag cgcagtcagc cttcacaatt agtccaatag
     3601 tggaaaagag ttgcatgaac aatttcgtca ttgccgcggt attgggggta gttgagggac
     3661 ttaccgagtt tttacccgta tcttccacag gccatctaat catcgcaggc catctgcttg
     3721 gtttcgttgg cgagaaagct gcaagctttg aggtggcgat tcagttgggc gctatcttat
     3781 cggtggtttg gctctattgg cgcaggtttg ttgcattgct gccgggtatg gcgcaatggc
     3841 cgttggcggc tgagtcgacc ttaattggct ggccgggcct ccagcgcatc gctttggcga
     3901 ccgtgcccgc tttaaccgtg ggctacctgg cgcgtcatgt catcaaagct caacttttca
     3961 atcccgaagc ggtgacactg gcgttggcga ctggcggcgt cgcgattctt ctagtcgagc
     4021 gtttaaccgc gcgccgatac gctactccag tggacgcggt gactctcggg caggctctag
     4081 gcgtcggact atttcaaatc ttggccctct ggccgggtac gtcgcgggcg gcagcaacca
     4141 taataggcgg aatgattctt ggacttgagc gcaaggcggc ggcggagttt tcgtttctga
     4201 tcgccgtccc cgtattgttc gtcgcttcag ggtatgagct tcttaagatg cgcgatcaaa
     4261 ttgttgccga cgatgcaatt agcctgacaa tcggattcgt gatttcgttc gtcgtcgcat
     4321 tgctagcgat taaagtattc gttagctact tgagccgtgg caagttggcg ccgtttgcct
     4381 ggtaccggat cgttgttgcg ccgatattct attttctcac caggggtgcg gctttgtgaa
     4441 tacgctgctt atttcagccg aagtgctaaa gcatcatcgc cacctttaat cgtgatcgtg
     4501 acgatgcttg atccagttaa aaatttgatt gcgatatata taaccgacga ctattgcgac
     4561 caaggtcact gccatgaaga tagcgaaaga gtggtactgc tgatttctaa tcgtcgcgcc
     4621 ttgcatcgtg agaagataag tcccggggat gcggccgata accgacacca ataagaaagt
     4681 agttacgctc atacggctca aaccgagcac gtaacaaaga taatccttag gaaaaccggg
     4741 aaacaggaac agcacgaagc agatgaccac gccggttttg gtggtgagga aattgaattt
     4801 ttcgagaacg tcgcgcttga tgaatcgttc aacaaacggt cgcccgagta tacttgccag
     4861 ctcgaatgcc acccaggatc cgattgacag gccaatggtg gatagaatga agccaatggt
     4921 ctcaccatag acaaagccac cggccactcc ggtgagctcg ccaggaaagg gagcggcgat
     4981 cacttgaaga atttgcagta ggacaaaaac cgccggcgaa taagggccta atgtttggag
     5041 aaactttccc agcttatcgc tgctggaata aatgccctcg aagtcgtcga gttcggcgcc
     5101 cggaggcgcg aaggcgctaa atggtcgcgg aatgtaacgg ctgaaaaaaa acagcagcaa
     5161 tatgagactg atgaagatgg ctccgcggat ggcgagccga cggcgctgat cgctcacccg
     5221 gcgctttata gcaactatta attctaaaga cgagagccac gcccgcgact tacactgcgc
     5281 tatcggagtc attgccttgc ttggttgagt cagggttgct acaaaacaca gttgaagttc
     5341 tgaaaatttc acattcatgt ttgccgattt gaaatgcggt actctagctg aactggaatc
     5401 gggccgcagt tggagcagcc aggaggtcgc tgttcgcgta gccagccgtg ccgacgtcat
     5461 gcgttcacat ggattgggcc gcggcgatcg cttgtttatc ctctatggca ataaactcga
     5521 attctttgtc gaccttctgg cggcatggca tgctggtgcg ggcgtggtgc cggtggacaa
     5581 tcgcttaacg cctttcgagg tggaaaactt agcacgggcg gtcactccaa aaatcgctct
     5641 agtcgatgcc acggtgccgt ccggactagg agacgcactt gcggcgatgg gagttaaagt
     5701 tctcagttcc gctgaaccaa cggagacgat tccagcggtg ttagcccata gcctagggtg
     5761 cgccgcacgg cttgacgacg aggctttgat cctgtttacc tctggcacta ccggcgtgcc
     5821 caaaggtgtg gttcatacgc atcggtcatt gcgcgcgcgc tggttcagtc tccgccagag
     5881 cttaggtatc gagtgttttc agcgcacgct ttgccttttg ccgacccatt tcggtcacgg
     5941 cttgatctgc aacagcttgt tcccatggct tttcggtcaa gatctcgtca tagcttcgcc
     6001 attcagtccg tacctgttgt tacgtctcgg gccgatcatc gatgagcata acatcacatt
     6061 tatgtcctct gtgccgtcga tgtggggctt gagtttaaaa ggtagcaagc cgccgcttgg
     6121 taaatcgctc cggcgcattc actgcggttc tgcgccgcta tcggcgcatc agtggcggca
     6181 aattcgagag tggtcgggaa ttcgtgcggt ttacaacgcc tatggtatca ccgaaaccgc
     6241 cagttgggtg gctggcacga gcatagaaaa cttcgagccg gaagatgggc tggtcggtga
     6301 accctggggc gcggcgatca agattctaaa gagtcccgaa cgtcaggcgc cgtttgccga
     6361 tgcgaacgag tgtaaagtta gcgagactgg atacgtctgg atcaatacgc cggccttgat
     6421 gcaaggctat ttcggccagc ccgagctcac cgatcaggtg atttccggcg gctggttcgc
     6481 gacaggtgat atcggattgg tcgatgaccg gggcagattt tatctaaaag ggcgtgagcg
     6541 agatgaaatt aataagggtg gagccaaagt ttatccgact gatgtcgatg cggtcgtcga
     6601 aagattcgag catgccatcg ctgtatgtac ttttgcgatc gctgatcccg cctatggtca
     6661 aaatgtcggt atggcggtgg tgttgagccg ccacgatgac gctactctac gtcagcttca
     6721 tgcatggctc aaagaacatt tagcagaatt caaaataccc cagcgctggt acgtcgtaga
     6781 cgagctgcca ctaaattcgc ggggaaaggt gagccgcaac acggtgcagg agctttgcgc
     6841 gcttctaaag cccgtggatt tggttggatt gttgcggcgc gtctcatgag ccgaa
//
LOCUS       DATLCH010000244         8533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7774599, whole genome shotgun sequence.
ACCESSION   DATLCH010000244 DATLCH010000000
VERSION     DATLCH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7774599"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VMZ02_07200"
     CDS             complement(<1..947)
                     /locus_tag="VMZ02_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71755.1"
                     /translation="MNRSLALIALCLYIAGSCMLAYFFPQSVLAFLLVEGSLLLLYYT
                     PVSGRVKSSFAALVLLVLLPILGFTNAYYFEIAIQIGIFVALALGLNIVVGFVGLLNL
                     GFVAFYAIGAYLWAVFGSPQANQFIAGGWFPLSPNWFFVFLILSIAVGALTGILLGLP
                     VLRLRGDYLAIVTLGFAEVVRALVNNLDKPINLTNGPKGIAPISKPPLFFKPVLEFFG
                     VQVADAALYPLYFYFLVLLLVFVIILVTRRLENSRVGRAWKAIREDEIAASAMGVPVV
                     RMKLLGFAVGASFASAVGVLFAAKQVFINPESFTFMESIG"
     gene            complement(944..1912)
                     /locus_tag="VMZ02_07205"
     CDS             complement(944..1912)
                     /locus_tag="VMZ02_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HUR71756.1"
                     /translation="MEIALEILPQVLLDGIVLGFMYALIALGYTMVYGVLEFINFAHS
                     EIFVLGAFVGVEVLLLLQSAGLLATLHPAVIVLLVIVTGMTISGLAAMTLERIAYRPL
                     MGGPRLVALISAIGASFFLQDGLRLFESLWRNTFYLNYPSLDFLDHNLPISASLVIPV
                     KSLLVIVTAILTLLGLHFFVNHTRMGMAIRAVAQDPDTASLMGVPVNRVIALTFFVGG
                     ALGGLAGVLFGIHYSLVNPYSGFVPGMKAFTAAVLGGIGNIPGAMLGGMLLGLLEAFA
                     ASYLSLLTDGAFGAEYKDVVAFVVLIVILIFRPKGLLGEAVRESKA"
     gene            2075..3646
                     /locus_tag="VMZ02_07210"
     CDS             2075..3646
                     /locus_tag="VMZ02_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HUR71757.1"
                     /translation="MSDAVTTPALALPAGGRKLKRGLLIGGGTIAALLVLAITLPHFV
                     DLGLFKQTYLPLIEDTLGRRVDVGEVHLRLVPTPSIQISKLRVSDNQSFAGNTFFAAQ
                     QIELKLRLLPLLRGRFEVIELVLDKPVFNLLKQADGSFNYSDIAAKKSSSSPRRQPRK
                     KLDGAGKGIEAMPLALPGRMRIHQGELNLVAKGQTPVNIRGIELALKDISGDSPFPFR
                     ASFQYPGLKSVALEGELDYQEEKALLEFKNNRLKIHDLTLPVQGSISNLAATPKVNLR
                     LNSDRVDAKPVFEVLSVFGLAPSDTEISGPMALNMTVAGPSNALVTEIRGVFNDVYVK
                     GKRALKGNLTGEVNIRLPLGGGRVSQRLQGNGKLVARDGELTNVNLIKKVQRVTGMIG
                     LTPDQQREATTFKTMSGDFTIGGGITDFSRLYLVNPQMEVTGNGTMTLEHPTLDILLN
                     TTLSPIASARTGRGRSVTAFKDGQGRIVVPLKVTGPVENPTVNLNAEKLGETGLPKTM
                     EKGFTSFFKNLFRGR"
     gene            complement(3685..4998)
                     /locus_tag="VMZ02_07215"
     CDS             complement(3685..4998)
                     /locus_tag="VMZ02_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HUR71758.1"
                     /translation="MVEQVMGTEFDELTRFKRGIHSAVKIVVMVWQDFFQNLVKLQAM
                     ALAFKTLLSLAPLLAVIFAILKAFGVHNRMEPALAQGLAPLGDKGREITVYLIGFVDK
                     MSAGALGTVGLVTLFVTVLSLMDTIEEGFNHIWRVRAARTLTRKFSDYLSALLVGPVL
                     VFAAVTITATLQNSAFVRGLLSFEALGKVLLILLWLVPYLTLWGALTFVYIFIPNTRV
                     RLRSAAVGGLVAAILWQSVGWGFARFVVSSTQYYAIYSSFAVLLLFLLWLHIGWVIVL
                     LGAQVAYAHQHIYFFQGDRQSLAQTPAGREKLALHAIHLVGRNFYYGLDPMTITDVAI
                     HMQIPAGMVKEFLEMFAETRLVLSVNDGSTYVLGRDPETITIKDVLDCVRNSGKKTKV
                     SENRSPEEDEIDELLATIDRSMTQALDGKSLQSLILSLPPPGKQV"
     gene            5308..6510
                     /locus_tag="VMZ02_07220"
                     /pseudo
     CDS             5308..6510
                     /locus_tag="VMZ02_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(6556..7146)
                     /locus_tag="VMZ02_07225"
     CDS             complement(6556..7146)
                     /locus_tag="VMZ02_07225"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HUR71759.1"
                     /translation="MTIDFTGRLLLLGLICANLGCTANSRSFDGNTLTLPRQVFLVRH
                     AEREWDGEDPPLVAAGVARAQALAATLRDAGISRIVTTQWRRTRDTVEPLARLLRITP
                     QVIPVFDGKARENVEQVAAAVRGYKDESVLVVGHITVTGVIAAIGGPSLPTICDNVFS
                     DLFLFKPAAGEAGLVHLHYGAAEDISPRCRLTPVSD"
     gene            complement(7195..7590)
                     /locus_tag="VMZ02_07230"
     CDS             complement(7195..7590)
                     /locus_tag="VMZ02_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HUR71760.1"
                     /translation="MEQKYFFSVLIIIGAVGVRFVMHFVDKGRIKDEVETKLGRVVSI
                     TWNPFGRGWFFEKGERHYDVTYVDRSGETKTTSCKTSLFTGVYWAEGPRAIDPPPRIM
                     SRHLCSKCGYSIQIEWRVCPNCGKPTEFA"
     gene            complement(7652..>8533)
                     /locus_tag="VMZ02_07235"
     CDS             complement(7652..>8533)
                     /locus_tag="VMZ02_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71761.1"
                     /translation="NWYIAKERQLFKKYGLDADLVFIPSSTTTIAAVLAGSVSVGNIS
                     GGATANAAVSGASVVAVGCFINTLPYDLVVHESIRSAQQLKGKAIGISRVGSSSDVAA
                     RAFLKELKLEPDKDVAILQVGGSTERAVAFRTGRIIAFPAPPGTIHLAHGMPHRVLIS
                     MADFPKGFPFPYVCPTASKSFVRQNRDTMKRLLMALIDGVHFYKTRKDESKKIMAKFT
                     RHSNEAFLEDAYQSSNKLFEQVPLVNREGMDVQVKDALSRKPGSALKVDDIVDDSLVL
                     ELEKEGFISRVYKQSVR"
BASE COUNT         2050 a         2440 c         2298 g         1745 t
ORIGIN      
        1 ccgatggatt ccatgaaggt aaagctctcg ggattgataa acacctgctt ggcggcgaac
       61 aggacaccaa cagcggaggc gaacgaggcg cctacggcga agccgagcag cttcatacgg
      121 actaccggaa ctcccatggc cgatgccgcg atctcatctt cgcggatcgc tttccaggcg
      181 cgcccgacac gcgagttctc caatctccgg gtgacaagaa taatgacaaa aaccagcagc
      241 aaaaccagga agtaaaaata cagcggataa agtgcggcgt cggcaacctg gacaccgaag
      301 aattccaata ccggcttaaa aaacagcggc ggcttggaaa tcggtgcgat ccctttaggt
      361 ccattggtca gattgatcgg cttatctaag ttgttgacta aagcgcgcac cacttcggcg
      421 aaaccaagcg tgacgatggc gagataatcg ccgcgcagcc tgagcaccgg taagcctaac
      481 agaatcccag tcagggcgcc gacagcgata ctgagtatca aaaaaacgaa aaaccagttg
      541 ggcgataacg gaaaccaccc gccggcaata aactgatttg cctggggcga gccgaatact
      601 gcccacaagt aggcgccgat ggcataaaat gcaacgaagc cgagattgag caaaccgacg
      661 aagccaacaa cgatgttgag tccgagggcg agagcgacga agatgccgat ctgaatcgcg
      721 atctcgaagt aatacgcatt tgtgaaaccc aagatcggca gcaataccaa cagtactaac
      781 gccgcgaaag agcttttcac tcggccgctc accggggtgt agtagagcag caacagtgac
      841 ccctcgacca acaaaaaggc caggacggac tgggggaaga aataggcgag catgcagctg
      901 cctgcgatgt agagacagag agcaatcaac gccaaagacc ggttcacgcc ttgctctccc
      961 gcacagcctc gccaagcagg cctttggggc gaaagattaa gatcacgatc aataccacga
     1021 atgccaccac gtctttgtat tcggcgccga aggcaccgtc ggtgaggagc gaaagatagg
     1081 acgccgcaaa cgcttccagt aaaccgagca gcataccccc gagcatggcg ccgggaatgt
     1141 taccgatacc gccgagcacg gccgcggtaa aggctttcat tccggggaca aacccggagt
     1201 agggattgac caggctataa tggatgccga acaatacacc tgccaggccg cccaatgcgc
     1261 cacccacgaa gaaagttagc gcgatgacac ggttgaccgg cacgcccatg agcgatgcgg
     1321 tgtcaggatc ctgggcaacg gcgcgaatcg ccatgcccat gcgcgtgtgg ttgacgaaaa
     1381 aatgcaatcc aagcagcgtt aagatcgccg tgacaatcac cagcagcgat ttcacgggga
     1441 taaccagtga tgccgagatc ggcaaattat gatcgaggaa gtcgaggctc ggatagttga
     1501 gataaaatgt attgcgccac aggctctcga acaatcgtaa gccatcctgc agaaaaaaag
     1561 acgcaccgat ggccgagatc aatgcgacca gcctggggcc acccatgagc ggccggtagg
     1621 cgatacgttc cagagtcatc gcggccaggc cgctgatcgt catgccggtg acgatgacca
     1681 gcaacacgat caccgctgga tggagcgttg ccagtagccc ggcgctttgc agaagcagga
     1741 gcacctcgac gcctacgaaa gcgcctaaga cgaagatttc gctatgggca aaattgataa
     1801 actccaaaac cccatacacc atcgtatagc ccagggcgat gagcgcatac atgaaaccca
     1861 gcacgatacc gtcgagcaaa acctgaggga gaatttccag tgcgatttcc aagtggttgc
     1921 gaacccttca gggacggcat gctaacaaat agttgtttgg ctagctaggt gttttgcagc
     1981 aaagtcggct gcaaagccat gcggcaacgc aagcggttat ttggattagc cggatattga
     2041 cgtataatgg ctccgatccc atcaacacgc cattatgtca gacgcggtaa ccacaccagc
     2101 actggcgctc cccgccggtg gcaggaaatt gaaaagaggc ctgctcattg gtggcggcac
     2161 aattgcagcc ctgctcgtcc tcgcgattac gctgccgcac ttcgtcgact tgggattgtt
     2221 taaacaaacc tatttgccgc ttatcgaaga tacgctcggc cgccgcgtcg acgtcggtga
     2281 agtgcatctc cgcctggtac ccacaccgtc gattcaaata tccaaactgc gcgtctccga
     2341 taatcagtcc tttgccggca acacgttttt cgccgcgcag cagatcgagc tcaaactacg
     2401 gctgttgcct ttgctgcgcg gccgcttcga agttatcgag ctggtgctcg acaagccggt
     2461 ttttaatttg ttgaagcagg ccgacggcag tttcaactac tctgacatcg ccgcaaagaa
     2521 atcgtcatcg tcgccgcgtc gccaacccag gaagaagctt gacggcgctg gcaaaggcat
     2581 agaagccatg ccactggcgc tgcccggtcg catgcgcatt catcaaggcg agctgaacct
     2641 cgttgccaaa ggccaaacgc cggttaacat cagaggaatt gagttggccc taaaagacat
     2701 ttccggcgac tcaccatttc cgttccgcgc atcgttccag tatcccggac tcaaaagtgt
     2761 cgccctggaa ggcgagttgg attatcaaga agaaaaagcg ctgctcgaat tcaaaaacaa
     2821 tcgtttgaag atccacgatc taacgttgcc ggtccaaggc agtattagca atctcgcagc
     2881 gacaccgaaa gtcaacctga gattaaatag cgaccgagtc gatgccaagc cggttttcga
     2941 ggtcctttca gtattcggcc ttgctccaag tgacacggaa atctccggtc ccatggcatt
     3001 gaacatgacc gttgccgggc cctcgaatgc cttggttacc gagatacgcg gagttttcaa
     3061 cgacgtttat gttaaaggca aacgcgcttt gaaaggcaac ttgacaggcg aagtaaacat
     3121 ccgattacct ctaggcggcg gacgagtgag tcagcgtttg caaggtaacg gcaaattggt
     3181 ggcgcgcgat ggcgagctga ccaatgtcaa tttgatcaaa aaagtccaaa gggtcaccgg
     3241 catgattggg ttgacaccgg accagcagcg cgaagcgacg actttcaaaa cgatgtccgg
     3301 cgatttcacc attggcggcg gcatcaccga tttctcgcgt ctctatttag tcaatccgca
     3361 gatggaggtc acgggcaacg gcaccatgac gttggaacat ccgacgctcg acatcctgct
     3421 caataccacc ttgtcgccaa tagcatcggc gcgcaccggg cgcggccgca gcgtgacggc
     3481 atttaaagac ggccagggac gtatagtcgt tcccctaaaa gtaaccgggc cggtggaaaa
     3541 tccaactgtg aacttgaacg cggagaagct cggcgagacc ggtctgccca aaaccatgga
     3601 gaagggcttc acgtcgtttt tcaaaaatct ttttcgcggc cgctgattcc ttaagaagct
     3661 gcgagagcaa gactgacagt gcgatcaaac ctgttttccc ggcggcggta ggcttaaaat
     3721 caaactctgc aaacttttcc cgtccaacgc ttgcgtcatg gaacgatcga tggtggcgag
     3781 cagttcgtca atctcgtcct cctccggact ccgattctcc gacaccttgg ttttcttgcc
     3841 cgagtttctg acacaatcaa gaacgtcttt gatggtgatg gtttccggat cgcggcctaa
     3901 aacataagta ctgccatcgt tcaccgacag caccaatcgg gtttctgcga acatttccag
     3961 gaattccttg accatcccag caggtatttg catgtggatc gccacatcgg tgatcgtcat
     4021 gggatctaat ccgtagtaga aattgcgccc gacgaggtga atggcgtgca gggccagctt
     4081 ctcgcgcccg gccggggtct gtgccaacga ttgacggtcg ccttgaaaga aatagatatg
     4141 ctggtgcgcg taagcgacct gagcgccgag caaaacgatc acccagccga tgtgaagcca
     4201 taggagaaac agcaacaaca ccgcaaaact cgaatagatc gcgtagtact gagtcgacga
     4261 cacgacgaac ctggcgaacc cccagccgac agactgccag agaattgccg cgactagtcc
     4321 gccgaccgca gcggaacgca gtcgcacccg cgtgttggga atgaaaatgt aaacaaaagt
     4381 aagtgcgccc cacagcgtta gataaggtac caaccacaat aatatcaaca gcaccttgcc
     4441 gagagcttca aaggacaaca acccacgcac gaaagcggag ttctgcaatg tcgctgtgat
     4501 cgtcaccgcg gcgaatacca acaccgggcc aacaagtagc gcgctcaaat aatcgctgaa
     4561 cttgcgtgtc agcgtgcgcg ccgcccgcac tcgccaaatg tgattgaatc cttcttcgat
     4621 ggtgtccatc aacgacagca cggtgacaaa tagcgtaacc aggcccaccg tgccgagtgc
     4681 ccccgcgctc attttgtcga cgaaaccaat gaggtaaacg gtgatctcgc ggcctttgtc
     4741 gcccagcgga gccaagccct gagcgagcgc cggctccata cggttatgca caccgaaggc
     4801 ttttaagatc gcgaaaatta ccgccaaaag cggcgccaat gacagcaggg tcttgaacgc
     4861 cagcgccatc gcctggagct tcaccaagtt ttgaaaaaaa tcctgccaca ccatgacgac
     4921 gattttgaca gcggaatgaa tcccacgctt gaatcgtgtc agttcatcga attcggttcc
     4981 catcacttgc tcgaccatgc gattcctcat ctgtcgaatc gatcgatttg cattaggagt
     5041 agactggcgg gtgggcataa attactgtca ttaggaataa acgcgagtca aacattatca
     5101 gctttgccct gcgccgacaa agcaatcgcg ctaattcgtt gacaaggcga aacatcgctc
     5161 gctatgctgc gccacaacat ggcatcatcc acggccgcat cgcctcacgg caaattccga
     5221 cgcgacctaa atctccccgg cttggttgcg ctgacttttt tttgcgtcgc tggcggcgcc
     5281 tagggacttg aggatggggt cggtgccgcc gggccattgc ccatgcttat cggcgtagcg
     5341 attcttccct ggctttggag cttgccgaca gcgttgatga ctgcggaatt atcgaccgcc
     5401 atgcccgagg atggcggcta cgtcgtctgg gtggaaaagg cgttcggccg cttctggggc
     5461 tttcaggagg gctggttgag ctggctgtgc agcttcgccg acaacgcgct ctacccggtg
     5521 atgttcgtcg attacctcgc ctatcttcgc ggcgaaatgg cgccgctgga gcgctggttg
     5581 atcgccgcga cgctcgtcag cgtcatcact tggctcaatc tccgcggtat ccgtttggtc
     5641 ggaatgcttt cggttgtttt caccgggctc gtgctcgcgc cttttgtcgt tatgctggta
     5701 atcggcttgc cgcaagtttc acccgccaac tggctgcaac gaactggcag caccgattgg
     5761 cctcttttgc tgagcatatt gttatggaac acctcgggct tcgacaacgc cggctgttgc
     5821 gccggtgagg tcaaggcgcc caatcgcgcg tacccgcgcg ccatggctct aaccgtgatc
     5881 ttagtcacgg cagcttactt gctgccgctg gcggtgggca cggggatcga cgccgattgg
     5941 cagcagtgga aagaaggcta ttttcctaag atcgcagcgc gaatcggcgg cccatggctc
     6001 ggcacctggc taaccgtggc ggcattggtc agtgccgctg gcatgttcag cgcgctactg
     6061 tgtacctcct cgcgcgtgcc attggccatg gcgcaacgca ggatgttacc gcgggcattg
     6121 gcggctactc atgcgcgcca cgccacgcca tggtgcgcga ttcttctcaa cagcgccggt
     6181 gttgccctgc tgatcccgtt ttcatttcag gaactgatcg aagtcgatat gtgtctctac
     6241 gccgcggcat tgcttttgga gttcgctgcg ctggtctggc tccggattaa acaacccgag
     6301 ttggcacggc cgtatagaat cccctacggc acgggtggcg tgatcgccct gtgcattccg
     6361 ccggtgttac tctgcatgct cagcatcgtg ctagcaaacc cagcgacaaa atacgtcggc
     6421 ctcggcggtg tcgctatcgg actactaata tatcgctggc agctgcggac cactgcgacg
     6481 acagagatcg cagccgcacc gattgtgtga gctatctgga aaattaacat gttagttagt
     6541 gaacgttgtg ccagcttaat cgctcactgg cgtaagtcgg cagcgtggcg aaatgtcttc
     6601 ggcagcgccg taatggagat gaaccaatcc tgcttctccg gccgccggct tgaacaaaaa
     6661 cagatcggaa aacacattgt cgcaaatagt cggcaaactt ggcccgccga tagcggcgat
     6721 aacgccggta accgtgatgt gtccgacgac aagcacggat tcgtccttgt agccgcgcac
     6781 cgccgccgcg acctgttcga cattttcccg cgcctttccg tcaaaaactg gaataacctg
     6841 gggtgttatc cgcaataatc tagctagagg ctctaccgta tctcgcgtgc gccgccattg
     6901 cgtcgtgacg atccgcgaaa tgccggcatc gcgcagcgtc gccgccagcg cttgagcacg
     6961 ggccacaccg gcagcgacca gcggcggatc ctcgccgtcc cactcgcgct ccgcatggcg
     7021 cacgagaaac acttggcgcg gcagcgtcag tgtgttgccg tcaaacgaac gcgagttcgc
     7081 agtgcaacca aggttggcac aaatcaaccc gagcagcaga agtcgtccag taaaatcgat
     7141 tgtcataaat cgttttgatt ttgcatcgag cgcatcctct gaaagagctt gaagtcacgc
     7201 aaactccgtg ggcttgccgc agttggggca aacacgccat tcgatctgaa tagaatagcc
     7261 gcatttcgaa cacaggtggc gcgacataat tcgcggtggc gggtcaattg cccggggccc
     7321 ttccgcccaa taaactcctg tgaacagact cgtcttacaa ctggttgttt tggtctcgcc
     7381 ggaacggtca acgtaagtga cgtcgtagtg gcgctcacct ttttcaaaga accatccccg
     7441 tccaaaagga ttccacgtga tcgagacgac tcggccgagc ttcgtctcga cttcgtcttt
     7501 gatacggccc ttgtcgacaa aatgcatgac gaaacgcacg ccgactgctc cgatgatgat
     7561 gagaaccgaa aaaaaatact tctgctccat tacgctccga tctcgtcagc tatcgtcctg
     7621 tgcccggtcc tgccacgaac tcacgaccca atcagcggac tgactgcttg tagactcgac
     7681 tgatgaagcc ttctttttcc aactccagaa ctaaactgtc gtcgacaata tcatccactt
     7741 tcagagcgct gcctggtttg cgcgaaagcg catctttgac ttgcacatcc atgccttcac
     7801 ggttgaccaa cggcacctgc tcgaacagct tgttgctgct ctgataggca tcttcgagaa
     7861 atgcctcgtt gctgtggcgc gtgaacttcg ccatgatctt tttgctctca tccttgcggg
     7921 tcttgtaaaa atgcacgcca tcgatgagcg ccatcaaaag ccgcttcata gtgtcgcggt
     7981 tctgtcgaac aaaacttttc gacgccgtcg gacagacata ggggaacgga aagcccttgg
     8041 gaaaatccgc catgctgatc aaaacgcggt gcggcatacc gtgagcgagg tgaatcgttc
     8101 ccggcggcgc gggaaaagcg attattcggc cggtgcgaaa tgccactgcg cgctcggtcg
     8161 agccgcctac ttgcaggatc gccacatcct tgtccggctc gagctttaat tctttgagga
     8221 aagcccgcgc cgccacgtcg gacgaactgc ctactcggct gatgccgatc gccttgcctt
     8281 tcagttgctg tgccgagcgt atcgattcgt gcacgactaa gtcgtatggc aaggtattga
     8341 tgaaacaacc gaccgccacc acgctcgcgc cgctcactgc cgcattggcg gtcgccccgc
     8401 cggaaatatt gccaacgctg acagacccag ccaacactgc ggcgatggtc gtcgtcgatg
     8461 atggaatgaa gaccaaatcg gcgtcaagac cgtatttctt gaagagttgg cgctccttag
     8521 cgatatacca gtt
//
LOCUS       DATLCH010000245         4906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9601146, whole genome shotgun sequence.
ACCESSION   DATLCH010000245 DATLCH010000000
VERSION     DATLCH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9601146"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VMZ02_07240"
     CDS             <1..270
                     /locus_tag="VMZ02_07240"
                     /inference="COORDINATES: protein motif:HMM:NF014640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HUR71762.1"
                     /translation="ENQQRVDELSIDLLRRFQDRVRQSKDLLAERAGRLGALSPLAVL
                     DRGYSIAHKLPEELIVRSSAQLKVGDLLRVSLAQGKAICRVEEKE"
     gene            272..1192
                     /locus_tag="VMZ02_07245"
     CDS             272..1192
                     /locus_tag="VMZ02_07245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HUR71763.1"
                     /translation="MKALAVSFMLVFLHAFVPLSFAAEVAKILWPKTLEAFQGDLVEV
                     QVGGDDLLEVNGRLGKETIYFHSSQQGVFSALLGIDVEAKPTVAKVILKATTHGGAHL
                     QREVPLKIKARAFQKESFTVAPSFDQMTPETLAEIRREQAAFAQAFANATAERYWSLP
                     FVRPVPQQASASSFGSRRIINGVPRTPHSGTDLSAPAGTEVVATNHGKVVLVGNYFYA
                     GGTVLIDHGGGLYSMYFHLSEIKVGEGVMVQRGDVLALSGGTGRVTGPHLHWGMRANN
                     SRVDPLDLLRKFSSQTESIGPENASANGEK"
     gene            1194..1448
                     /gene="xseB"
                     /locus_tag="VMZ02_07250"
     CDS             1194..1448
                     /gene="xseB"
                     /locus_tag="VMZ02_07250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HUR71764.1"
                     /translation="MAKKESTEQNFEQALAELEEVVEQLESGALPLDNSLAAFEKGVG
                     LVKFCNQKLNEVEKKVELLLKDKDGRLQLTTLGEIDDIGE"
     gene            complement(1466..2155)
                     /locus_tag="VMZ02_07255"
     CDS             complement(1466..2155)
                     /locus_tag="VMZ02_07255"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HUR71765.1"
                     /translation="MSFTYLLINLGIIAGPLWLSRDRDVAYQRNFPALAGATIIVGTL
                     YILWDLFATAQGDWSFNPLYVTGVNIKNLPVEEICFFVTVPYACLFIYEILARSAQEK
                     RLCLPTAVLWAAVGALLAGAWLFANQSYTAKALSSCALFLGFALWLDRPLLNSRHYWL
                     WLAICLIPFAIVNTILTALPVVQYNSSAIWGVRIGTIPVEDFFYNYSLLSFYLLSYRL
                     IAQHLGRSRSA"
     gene            complement(2171..3691)
                     /locus_tag="VMZ02_07260"
     CDS             complement(2171..3691)
                     /locus_tag="VMZ02_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR71766.1"
                     /translation="MSFFSHHVKSYIKRRLHGYREVVNDVDITRPKQLAGHKAVAVVG
                     AGLAGIAAAALLAERGFAVTLFDRNPYLGGKTAGWQVTFADGTTTSVDHGFHAFFRHY
                     YNLQAFLEKIGARQYLSRIDDYLVLGADGQRFGFKDVATTPVLNIISLGLNKFYRFRD
                     VLLNPGSRRMGEFIKYDEIKTYGAWDHVSFAQFAEEAKLPSALRLVFNTFARAFFAPA
                     EKLSSAELMKSFHFFYLSHDEGLLYDYFITDYERAFVRPVSDYLAQRHVRLKLNHPIT
                     HTTREAGRFIAGGESFDAIILACDVRGSREICRNSEWLQRESPSDYDKLINLEASGGY
                     AVYRLWLDRQTDPALPVFVITEKRRVLDSVTFYHRFEANSLQWAKECGGGVYELHCYA
                     LPEDIDTEESLQAAFLDELHFYFPDLKGARILRQHLQMKRDFTAFHTGLHEHRPSTTT
                     GVANFFLAGDWVKIHTPAMLMEGAFTSGLIAANHLLAQEGLQEEPIHSVPRRGILG"
     gene            complement(3688..4794)
                     /locus_tag="VMZ02_07265"
     CDS             complement(3688..4794)
                     /locus_tag="VMZ02_07265"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HUR71767.1"
                     /translation="MNDPAILEKCGQDNCATPIRRVGINLNLWYPVALSSEVGSRPVG
                     RELWHQPIVLFRTSNGRLGALEDRCAHRFVRLSHGRLIDGGEDSLECAYHGWRFDISG
                     RCVHIPHLAGKPVLPNCSVRAFPVLEKYGFVWIYPGEPSRAHEVAPTAMTEWDDPNEI
                     RSVARFSCRAHFSYLIENLMDMYHGKLHAEHQVWIANELTKVEQSDRHVSATYNATTF
                     YRVTSLSSILQLILPALRKPSAAPLTVTYEYPNWRSTLGEDFKIYCLITPVHERLTEA
                     HLIHYTSLSKFRALTTAPMVVRLKVKSLLNNCAKRLLDNLAREDVDMIEEEQTVFDMQ
                     PERQPFEVNRTLLRVQELIRRQESAAQPIGQDRP"
BASE COUNT         1147 a         1263 c         1441 g         1055 t
ORIGIN      
        1 gagaatcagc agcgtgtcga tgaattgtcc atcgatcttc tgcgccgctt ccaagatcgc
       61 gtgcgtcagt caaaggacct tttggcagag cgcgcgggaa gattgggcgc tctcagccct
      121 ttagcggtgc ttgatcgcgg ctacagcatt gcgcacaagc tgcccgagga gcttattgtg
      181 aggagttcgg cgcaactgaa agtgggcgat ttgttgcggg tcagcttggc ccaaggtaaa
      241 gcgatttgcc gcgtcgaaga gaaagaatga aatgaaagcg ctggccgtgt cgtttatgct
      301 cgtgtttctg catgcgttcg tgccgctatc gttcgctgcc gaagtggcga aaattctctg
      361 gcccaagaca ctcgaagcct tccaaggaga ccttgtggag gttcaagtcg gtggtgacga
      421 tttgcttgaa gtgaacggcc gtttgggcaa ggaaacaatt tatttccatt cgagtcagca
      481 gggagtcttt tccgcgttgc tgggcatcga cgtcgaagca aaaccgacag tcgcaaaagt
      541 aattttgaag gcgaccaccc atggcggtgc gcaccttcag agggaggttc cgctcaagat
      601 caaagcgaga gccttccaaa aggagtcttt caccgtggcg cccagtttcg atcagatgac
      661 gccggaaacc ttggccgaaa ttcgccgcga acaggcggcg tttgcgcagg cttttgccaa
      721 tgcgacagcg gaacggtatt ggagtttgcc gtttgttcgt cccgtgcctc agcaggcctc
      781 ggcctcgtcc ttcgggtccc ggcgcatcat aaacggcgtg ccgaggacac cgcatagcgg
      841 gaccgatcta tccgcgcccg cgggaaccga ggttgtcgct accaaccacg gcaaggtggt
      901 gctcgtcggt aattactttt atgccggtgg caccgtcctt atcgatcacg gcggcggctt
      961 gtactcgatg tattttcatc tgtcggagat caaagtagga gagggcgtta tggtgcaacg
     1021 cggcgatgtg ctggcactct ccggtggcac cggacgggtc acggggccgc atctgcactg
     1081 gggtatgcgt gccaataaca gccgtgtcga tccgttagat ttattgcgta aattttccag
     1141 tcaaacggaa tcgatcggtc ccgagaacgc ttcggccaat ggagagaagt gacatggcaa
     1201 agaaagaatc gactgaacag aattttgaac aggctttggc agaattggaa gaggtagtcg
     1261 aacagctgga atccggcgcg cttcctctcg acaactctct ggccgctttt gaaaagggcg
     1321 tgggcctggt gaagttttgc aatcaaaaat tgaacgaggt tgagaaaaag gtggaactct
     1381 tgctgaaaga caaagacggt cggctccagc tgacaaccct gggggaaatc gatgacattg
     1441 gcgagtaact attgccgatt tgctgctaag cggatcttga cctgcccagg tgctgggcga
     1501 tgagtcggta actcaaaaga taaaacgaaa gtagcgaata attataaaag aagtcctcaa
     1561 cgggaatagt gcctatgcgc accccccata tcgcactact gttgtattga accaccggca
     1621 gcgcggtaag aattgtattg acgatcgcga acggaatcag gcaaatggcg agccagagcc
     1681 aatagtgacg gctgttgagc aggggccggt ccaaccatag agcaaatcct agaaacagag
     1741 cacaggagct caaggctttc gcagtgtaac tttggttcgc gaatagccaa gcgccggcaa
     1801 gtagagcgcc gactgcggcc cacagcacag cggttggcaa gcacaagcgt ttttcctgtg
     1861 ccgacctcgc gaggatttca taaataaaca agcaggcata aggcaccgtg acgaaaaagc
     1921 agatctcttc cacgggaaga ttcttgatgt tgaccccggt gacataaagc ggattgaaac
     1981 tccaatcgcc ctgcgccgtg gcgaacagat cccagaggat atacagcgtg ccaacgatga
     2041 tcgtcgcgcc tgccagcgct ggaaaattgc gctggtaggc gacatcgcgg tcgcggctca
     2101 gccaaagcgg accggcgata atgcctaagt tgatcaacaa gtaggtgaac gacatgaacc
     2161 tagaacgttt ctaacctaaa attccacggc gcggcaccga gtggatcggt tcttcttgta
     2221 ggccttcctg ggcaagcagg tgattcgctg cgatcagacc ggaggtaaat gcgccctcca
     2281 tgagcatcgc cggcgtgtgt atcttgaccc agtcgccggc taaaaaaaaa tttgccactc
     2341 cagtcgtcgt cgatggtcgg tgttcgtgca agccggtgtg aaatgccgtg aaatcgcgct
     2401 tcatttgcag atgctggcgt aagatacggg cgcccttcaa gtcgggaaaa taaaagtgca
     2461 attcgtcgag aaaagcggct tggagcgatt cttcagtgtc gatatcctcc ggtaaggcat
     2521 agcaatgaag ctcgtaaacg ccgccgccgc actctttggc ccactgtagg gagtttgctt
     2581 caaagcgatg gtaaaaggtg actgagtcga gcacccggcg tttctcggtg atgacgaaca
     2641 ccggtagtgc gggatcggtc tgccggtcta gccacagccg atagaccgca taaccgccgg
     2701 atgcttcgag gttgatcagc ttgtcatagt cggatggaga ctcgcgctgc agccattccg
     2761 agttgcggca aatttcgcgg ctaccacgaa catcgcaggc gaggataatc gcatcgaagg
     2821 attccccgcc ggcgataaat cgtcccgcct ctcgtgtcgt atgggtgatc gggtggttga
     2881 gcttcaagcg cacatgccgc tgcgccagat agtcgctcac gggtcggacg aaagcgcgct
     2941 catagtcggt gatgaaatag tcataaagca atccctcatc atggctcaag taaaaaaaat
     3001 ggaagctctt catcagctcc gcgctgctga gcttttcggc cggggcaaaa aaggctctgg
     3061 caaaggtgtt gaacaccaag cgcaatgctg aaggcagttt ggcttcttca gcgaactgcg
     3121 caaagctaac atgatcccaa gcgccgtagg ttttaatttc gtcgtatttg ataaactcac
     3181 ccatgcgtcg actgccggga ttcaacagca catcgcggaa tcggtaaaat ttattcagac
     3241 ccaaggagat gatgttgaga acgggcgtgg tggcaacgtc tttaaagccg aaacgctggc
     3301 cgtctgcgcc caggaccaga tagtcgtcaa tccggctcaa gtattgccgc gcaccaattt
     3361 tctccaggaa cgcctgcaga ttgtaatagt gacgaaaaaa ggcgtgaaag ccatgatcaa
     3421 ccgacgtcgt ggtgccatcc gcaaaggtca cttgccaacc ggcggtcttg ccgccgagat
     3481 aggggttgcg gtcaaacaga gtgaccgcga agccgcgttc ggccagtagg gccgccgcgg
     3541 cgatgcccgc caggccggcg ccgaccacgg ctacggcttt atgacccgcc aactgctttg
     3601 gtctcgtaat atcgacatcg ttgaccacct cgcggtagcc gtgcagtcgg cgcttgatgt
     3661 agcttttaac gtgatgcgag aaaaaactca cggtctgtcc tgtccaatgg gctgcgccgc
     3721 gctttcctgg cggcgaatca gctcttggac gcgcaagagc gtgcgattga cctcaaaggg
     3781 ttgacgctcg ggctgcatgt cgaaaaccgt ttgctcttct tcgatcatgt cgacatcttc
     3841 gcgtgccaaa ttatcgagca ggcgcttggc gcaattgttg agaagactct tgaccttgag
     3901 gcgcacgacc atgggtgcgg tggttaaggc tctaaacttg ctcaacgacg tgtagtggat
     3961 cagatgggcc tcggtcagtc gttcatgcac cggggtgatg aggcaataga ttttgaaatc
     4021 ttcacccaac gtcgagcgcc aattgggata ctcgtaagtg accgtcagcg gcgcggcgga
     4081 gggcttgcgc agtgcgggga gaattaattg taagatcgag ctcagactgg tgacgcggta
     4141 aaatgtcgtg gcgttgtagg tcgcgctcac gtgacggtca ctctgttcga ctttcgtgag
     4201 ctcattggct atccagactt gatgctcggc gtgaagcttg ccgtggtaca tgtccatgag
     4261 attctcgatt agataggaga agtgggcgcg acagctgaag cgcgcaacac tacgaatctc
     4321 gttcggatcg tcccattccg tcattgccgt cggggcgact tcatgggcgc ggctcggttc
     4381 tccgggatat atccagacga atccgtactt ttccagcacg ggaaaggcgc gaaccgagca
     4441 gttcggcagg accggcttcc cggccaagtg cggaatgtgg acgcagcgcc cagagatatc
     4501 gaaacgccag ccatgataag cgcactcgag cgaatcttca ccgccatcga tcaatcggcc
     4561 atggctcaag cgcacgaaac gatgggcgca gcggtcttca agcgcgccga ggcgaccgtt
     4621 gctggtgcga aacaggacaa tcggttgatg ccaaagttca cgccctacgg gtcggcttcc
     4681 aacctccgac gagagcgcga cggggtacca gagattgaga ttgatgccaa ctcggcgaat
     4741 cggtgttgcg caattgtcct gaccgcattt ctctaaaatg gcggggtcgt tcattttgat
     4801 gatgtctctt gcgtagccaa ggaaatggga cttcagctca cggcgccggc gtggcgagcg
     4861 gccttgacag ttcattgacg cactgctcga tatttgcaat cagtgt
//
LOCUS       DATLCH010000246         5756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5875277, whole genome shotgun sequence.
ACCESSION   DATLCH010000246 DATLCH010000000
VERSION     DATLCH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5875277"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VMZ02_07270"
     CDS             complement(<1..946)
                     /locus_tag="VMZ02_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71768.1"
                     /translation="MTQILLFTLLFLSSAAHAQDAKLIEAARKDGKVIVYGTMQSDIF
                     ELLQKLFQKKTGLTVDYWRTSATKVMERALTEARAGKALFDLVMSTEDTQRIMLKEGI
                     LAKYDSPMARDFPKDAVEPQLGPRARNHIVGVMYNKNLIKPADAPKTLEDLVKPQFRG
                     KLVMADPTLHTTTAQWLANLPKLMGKDRSEKFIRELAAMKPVLVESFGPAADRITTGE
                     TPIGITFVRFVLTYNKQGASLDYVRDYTMLGDGQYISLAAKAPRPNAGKAFIDFFLEE
                     ESMKIQADTGEFVNRRGVYPPLQDADKIKFVQMYSFGKE"
     gene            complement(1058..2098)
                     /locus_tag="VMZ02_07275"
     CDS             complement(1058..2098)
                     /locus_tag="VMZ02_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71769.1"
                     /translation="MLKPKVIDADGHIYENHEEIEEYFEGKYRGMRRARAYALFPSLD
                     GWPRGLGAGRPDKVTETKPEDVIKFVETLGVDYTVLYPTAALAIGLVQDPDWSCSISR
                     AYNNWFNARYIKRDSRLKGVAVLPIQDPLGAAAELRYAVEKLDMVGAFLPSAMVMSKA
                     LGHRDFRPIFAAAQDLGVPLGIHGAPSRGFGFDFFEDQGQVHALEHPFPLMIQFTSII
                     CDGVLEQFPKLKIAFLEAGCGWLPYMMDRLDYEYETRGARSFPNIKKKPSDYVKDCPV
                     YVTCEPEETSLAYVAQVLGDDRIMFPSDYPHERVYGEFLKDIPELLRRPELSDATKQK
                     ILYDNAKEFYRI"
     gene            2377..3462
                     /locus_tag="VMZ02_07280"
     CDS             2377..3462
                     /locus_tag="VMZ02_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71770.1"
                     /translation="MARNGIPVVDGDGHVMEDWDALLQYMPDPYIKSGRFKGRIFPPL
                     DHLHSATLYQLVPGAFKAVREDGWLEFMEDVGIERAVLYSTGGLAFGKVITREFAIDV
                     ARAWNNWFADTYLKKSPRFQGLALVPLQELPEAIKEMHRAVTELKFCGVMLPSTGFKG
                     HLGDKEYWPFYAEASKLGCCIGVHGGAHEGLGMDWLTPYAPINGLGHAFSQMVAFAGI
                     VFNGIFEKYPNVRIGFMEAGVSWLQTCLERFDRGWETHIQYDPRSEYLQLRPGEKVSD
                     YIRRHVEAGRIFVGCEGTEKGLHHLIEAVGNKPFMFSSDFPHEVNNEYCKHEIEEIID
                     NDHLCDADKHAILHGNAERFYGLKPLG"
     gene            3509..3796
                     /locus_tag="VMZ02_07285"
     CDS             3509..3796
                     /locus_tag="VMZ02_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71771.1"
                     /translation="MMENLIEIVNPLGVPRAQVSKLAPRRHRSLTGRRVGLLDNNKPN
                     ADKFLQLIGEMLQARYAELTLLAKCKMTRMESDGLVQLAESCDVVINAFAD"
     gene            3866..5128
                     /locus_tag="VMZ02_07290"
     CDS             3866..5128
                     /locus_tag="VMZ02_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HUR71772.1"
                     /translation="MVSSEFWTLALAESQARGVKDLPLIKVPHPVGTISLEALHAVAE
                     SVVDAIAGKLMRGSDSTSAGNRMASQQDEGSAAASLTVPNDPAEMFSFFCDRGWTDGL
                     PVLPPTLAAVQKMIAAGGNKPDVVLGIIPPLNGVATVEKVAANAVMAGCLPNYFPLVL
                     AAVRAMARPGYNLDGVQTTTGNIAPLAIVNGPCRNQLAINYGANVLGQGWRANATIGR
                     AIRFVMTNIGGARPGSFDKSTVGQPAKYTFCFAENEEASPWQPLHVERGLARERDTVH
                     MFGASGVYSAVDMASQSATGLLKTYSLTMTGGLASGVTSTEVLWVICPEHAAILERDG
                     FSKDKLRVELFARARIPYDNIADENLALLAKRRPLWFKSGALREIGVVDRPEDIWLVV
                     AGGAGAKSAYIPGRTGTRMQTAAVEGFD"
     gene            5128..>5756
                     /locus_tag="VMZ02_07295"
     CDS             5128..>5756
                     /locus_tag="VMZ02_07295"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71773.1"
                     /translation="MLYGNDGFIIRIRQITITLIYASMLVGPVLTYGQSKSIIIGEGV
                     RGAMYLPAYIAEEKGFFKRRELDTRMVTFSRSNDINALVSGDIQFDLTAPDKVVHSAL
                     GGFPVKMVMGTARGLNLALTVHPSIRSAADLKGKSVAITGFSGLPYTGLLLCLRELGM
                     TKDQVVPLNIGGKTARFEALLGNRVPAAILDPPYTTLAAKEGYRLLVDLA"
BASE COUNT         1226 a         1502 c         1693 g         1335 t
ORIGIN      
        1 cttccttgcc gaaactatac atctggacga acttgatctt gtcggcatcc tggagcggcg
       61 gataaactcc ccggcgattg acgaactcgc cggtgtcggc ctgaatcttc atgctttcct
      121 cttcgaggaa aaaatcgata aacgcttttc ctgcattggg tctcggtgct ttggcggcaa
      181 ggctaatata ctgtccgtcg cccagcatcg tgtagtcacg cacataatcg agactcgctc
      241 cctgtttgtt gtaggtgagc acaaagcgca cgaaggtgat gccgatgggc gtctcgccgg
      301 tggtgattcg atccgccgcc ggaccgaagg attcgacgag caccggcttc attgccgcta
      361 gttcgcggat gaatttctcg gacctgtctt tgcccatgag cttgggcaaa ttcgccagcc
      421 attgagcggt cgtagtatgc aatgtcgggt cggccatgac cagcttgccg cggaattgtg
      481 gcttgaccaa atcttccaat gtctttggcg cgtcggcggg cttgatcaaa tttttgttat
      541 acatcacacc gacgatgtga ttacgggcgc gcggtccgag ctgcggttcg acggcgtctt
      601 ttggaaaatc cctcgccatc ggagaatcat actttgccag gatgccttcc ttgagcatga
      661 tgcgttgggt gtcttcggtc gacatcacta gatcgaaaag cgccttgccg gcgcgcgctt
      721 cagtcaatgc gcgttccatc accttggttg ccgaggtgcg ccaataatcg acggtgagac
      781 cggttttctt ttgaaagagc ttctgcagga gttcgaaaat atcggattgc atggtgccgt
      841 agacgatcac cttgccgtcc tttctcgcag cttcgatgag tttggcgtcc tgggcgtggg
      901 cggcactact gagaaataat agagtgaata aaagtatttg cgtcatggag agtgaacctc
      961 gtattgccga tttcttgtgg caacctcggc ggtcgccaat ctgaacatct gtagaagcga
     1021 gacccgggcc tccgtctact cgggccatgc cgcgggctca gattcggtaa aattctttgg
     1081 cgttgtcgta gaggattttc tgtttggttg cgtctgacag ttccggccgt ctgagcagct
     1141 ccggtatgtc cttcaagaat tcaccataga cgcgctcatg cggatagtcg gaggggaaca
     1201 tgatgcggtc gtcaccgagc acttgtgcga cgtatgccaa agaagtctct tccggctcgc
     1261 aggtgacata gaccgggcaa tcctttacgt agtcgctggg cttctttttg atattgggga
     1321 aagaccgcgc gccgcgagtt tcgtattcat aatccaaacg atccatcatg taaggcaacc
     1381 agccgcagcc ggcttcgaga aaagcgattt tcaatttcgg aaattgttcg aggacgccgt
     1441 cgcagattat gctggtgaac tgaatcatca acggaaaggg atgttccagg gcatgaactt
     1501 gtccttggtc ttcgaagaaa tcaaaaccga aaccgcggct gggtgcgccg tggatgccga
     1561 gcggcacgcc gagatcttgc gccgcagcga aaatcggtcg aaaatcccga tggccgaggg
     1621 ctttgctcat caccattgcc gacggcagaa acgcgccgac catatcgagt ttctcgacgg
     1681 cgtagcgcaa ttccgcggcc gcgcccagtg gatcttggat cggcaggacg gcgacaccct
     1741 tcaagcgcga gtcacgtttg atgtagcggg cattgaacca gttgttgtaa gcgcgcgaga
     1801 tcgagcagga ccaatccgga tcttgcacca agccgattgc taaggcggct gtcggataaa
     1861 gcacggtgta atcgacgccg agggtttcaa cgaatttaat cacatcttcc ggtttcgttt
     1921 cagtaacctt atccggacga ccggcgccga gaccacgcgg ccaaccgtcg agtgatggaa
     1981 acagtgcata ggcgcgggcg cggcgcatgc cacggtattt accttcaaaa tattcttcta
     2041 tttcttcatg gttttcgtaa atatggccgt cggcgtcgat aacttttggt ttcagcataa
     2101 aagatattcc ttctggtcgt ttctaatcgg aatgcgcgag gttaggctgc gcctcggttt
     2161 gctgtcaaga ggattgatct gatgttgggc aacaactcaa ttgaaggctg agaagacgaa
     2221 gcagagatac ccgcacacaa tacaacgccg gcgtaattca ttatgccatc gcatcactct
     2281 ctttgggatt tgccctgctc tgtacgcatt gattgcggca gtggttttgg gttagacaat
     2341 aggaaactag cttctagcta atggaggaaa tagtttatgg cccgcaacgg aatacccgtc
     2401 gtcgatggcg acggccacgt gatggaagac tgggacgcgc ttttacaata catgccggat
     2461 ccttacatta agagcggccg cttcaagggt aggatcttcc caccgctcga tcatctgcac
     2521 tcggcaacgt tgtatcaatt ggtgccggga gctttcaaag ccgtgcgcga ggacggctgg
     2581 ttggaattca tggaagatgt cggcatcgag cgcgccgtgc tctacagcac cggcggcttg
     2641 gctttcggca aagtaatcac ccgcgaattt gccatcgacg tggcgcgggc ctggaacaac
     2701 tggttcgccg atacgtattt gaaaaaaagc ccccgttttc aggggttggc gttagtgccg
     2761 ctgcaggaat tgccggaagc tatcaaagaa atgcaccgcg cggtcacgga gcttaagttt
     2821 tgtggcgtca tgctgccgtc gactggcttc aaagggcatc ttggcgataa agaatattgg
     2881 ccgttctacg ctgaagctag caagctcggt tgctgtattg gagtccatgg cggcgctcac
     2941 gaaggattgg gcatggattg gcttacgccg tacgcgccga tcaacggctt gggccatgcg
     3001 ttcagccaga tggtggcgtt tgccggcatc gtcttcaacg gtattttcga gaagtatccc
     3061 aatgttcgca tcggcttcat ggaagctggc gtgtcttggc tgcagacttg tctagagaga
     3121 ttcgaccgcg gttgggagac tcacattcaa tatgatccac gcagcgagta cctccagctc
     3181 aggcccggcg aaaaggttag cgattacatt cgccgccatg tcgaagcggg gcgcatcttc
     3241 gtcggctgcg agggcaccga aaaaggcctg caccatttga ttgaagcggt cggcaataag
     3301 ccgtttatgt tctcgagcga ctttccgcat gaggtcaata acgaatattg caagcatgag
     3361 atcgaagaga ttattgataa cgatcatctc tgcgacgcag acaagcacgc cattttgcat
     3421 ggcaacgctg agcgttttta tggactcaag ccactgggat gacccgtggg tccgcggaag
     3481 cgcacgtttg ggctgcgaaa ggtgaccgat gatggaaaat ctgatcgaaa tcgtcaatcc
     3541 gctcggggtc cctcgcgcgc aagtgagcaa actggcgccg cgccgccacc gcagtctgac
     3601 cggccgtcgc gtcggcttgc tggacaacaa taagcccaat gcggataagt ttcttcagct
     3661 gatcggcgaa atgctgcaag cgcgctacgc cgagctaacg ctgcttgcca aatgcaagat
     3721 gacgcgcatg gagtccgacg gtttggtgca gctggccgaa agttgcgacg ttgtcatcaa
     3781 cgcctttgcc gattgaggct cgtgcacgtc gtggagtatc cacgactccg tcaaactcga
     3841 agctgacgga atccccactg cgactgtggt cagcagcgaa ttctggacgt tggcacttgc
     3901 ggagtcgcaa gcccgtggtg tgaaagatct gcctctgatc aaggtgcccc atccagtggg
     3961 gacgatatcg cttgaggctt tacacgcggt tgccgaatcg gtggtcgatg cgattgccgg
     4021 caagctaatg cgaggcagcg attcgacttc agctggtaat cgcatggctt cgcagcagga
     4081 cgaaggcagt gccgccgcca gtttgacggt ccccaatgat cccgccgaga tgtttagctt
     4141 cttttgcgat cgtggttgga cagatggctt acccgtgttg ccgccgacgc tagcggcagt
     4201 gcaaaaaatg attgctgccg gcggtaataa accagatgtg gttttgggga tcattccgcc
     4261 gctgaatggc gttgctacgg ttgaaaaggt tgcagccaat gccgtgatgg cgggctgtct
     4321 gccgaactat tttcccctcg tgctcgcggc ggtgcgtgcg atggcacggc cgggttacaa
     4381 cctcgacggc gtgcagacga ccactggcaa catcgctccc ctggcgatcg ttaatggacc
     4441 gtgccgtaat cagttagcga tcaactacgg tgccaatgtc ctcggccaag gctggcgcgc
     4501 gaacgctacc atcggccggg ccattcgttt cgtcatgacc aatatcggcg gcgctcgacc
     4561 gggcagcttc gataaatcga ctgtgggcca gccggcaaaa tacacgtttt gcttcgccga
     4621 gaatgaagaa gcgagcccgt ggcagccgct tcatgtcgag cgcggcctgg cgcgagaacg
     4681 cgatacggtt catatgttcg gcgccagcgg cgtttatagc gcggtcgata tggcgagcca
     4741 gtcagcgacc gggttgctca agacgtattc tctgacgatg accggcggct tggcgagcgg
     4801 cgtgacttca accgaagtcc tctgggtgat ctgcccggaa catgcggcca tcctagaacg
     4861 agacggtttt tcaaaggaca aattgcgcgt cgagttgttc gcccgcgcac gcattccata
     4921 cgacaacatc gccgatgaaa atttagcact gctcgccaaa cgccggccgc tttggttcaa
     4981 atctggtgcg ctgcgcgaaa ttggcgtggt cgatagaccg gaggatattt ggttggtggt
     5041 tgccggcggc gccggcgcca agtcggcata tatacccgga cggacgggaa cgcggatgca
     5101 gacagcggcc gtggagggat ttgactgatg ctgtatggca atgatggatt tattattcgt
     5161 attcggcaga tcacgataac cttaatttat gcctctatgt tagtggggcc ggtgctcacc
     5221 tatgggcaaa gcaaatcgat catcatcggt gaaggtgtgc gcggagcgat gtatctaccg
     5281 gcgtacatcg ccgaggaaaa aggcttcttc aagcggcgcg agttggatac ccgaatggtg
     5341 acattcagcc gtagcaacga tatcaacgcg ttggtgtcgg gcgatataca attcgacttg
     5401 acggcgccgg acaaagtcgt ccatagcgcc ttgggaggct ttccggtgaa gatggtgatg
     5461 ggcacggcgc gcggattgaa tcttgccttg acagtgcatc cgtcgatccg ctcggcggcc
     5521 gatctaaagg gcaagtctgt ggcgatcacc ggcttttccg gattgcccta cactgggcta
     5581 ctgctttgtt tgcgtgagct cggcatgacc aaggatcagg tcgttccgct caatatcggc
     5641 ggtaagacgg cgcgcttcga agccttgctc ggcaatcgtg tacccgcggc catcctcgat
     5701 ccgccctaca ccacgctcgc ggctaaggaa ggctaccggt tgctggtgga tttggc
//
LOCUS       DATLCH010000247         4052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5138097, whole genome shotgun sequence.
ACCESSION   DATLCH010000247 DATLCH010000000
VERSION     DATLCH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5138097"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VMZ02_07300"
     CDS             complement(<1..777)
                     /locus_tag="VMZ02_07300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71774.1"
                     /translation="MNTAQDFFKSIGALGKERIWLLGVLTVGHFVIHWFQQFFPVLLP
                     SIKSGLNLSNVEVGALTSAQQVVVGLGQLPLGMVADAMVRHRATILALSLIAMGAAYF
                     LLALPAFSWALLGSAMIGFGTALWHPTAAASLSNQFPERRATALSIHGTGATISDTIT
                     PLLVGILLANLSWQAATQLQLFPGVLFAFLLWRALAGVFRDSAAPRQRIAAQFRGVAT
                     IIKNPAFLGLSVATGLLSMSRLIILTFLPIYLQEHLHYSSV"
     gene            1009..2007
                     /locus_tag="VMZ02_07305"
     CDS             1009..2007
                     /locus_tag="VMZ02_07305"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71775.1"
                     /translation="MKLSIFAYLAFVIFLAIYHDSPAQIAASPTKVVMTIGAISEREG
                     VVYVAQDQGFFRKHGLDLTLVQVRNGAVGIAALSSGESQVHWGSVSAANLGAIAEGTD
                     LVFLAGFINKLTGAFVVNPKIKSPAELKGRNIGVNGLSGGAWIFAMLTLDYWGLVPER
                     DNIQFRALGDQGVIAQGLAAGAVDAAFLGYTFGKALESKGFRILADVERLPIPYQGSG
                     IMARRSFALSSPAIVENVLRGLLDSLAFIRDPANKAAVLKSLAKGLRLKRVEEAEEGY
                     QIMVGGLYEKKIYPSVDGIRNVIRLLGKTNEKIRRLKAEDLVNDSSVKKLEKEGRF"
     gene            complement(2210..2854)
                     /locus_tag="VMZ02_07310"
     CDS             complement(2210..2854)
                     /locus_tag="VMZ02_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HUR71776.1"
                     /translation="MAYSLVHQVPSFVEVSMNEVPLRLVIWLIFSLFALPACDRDKEI
                     QAMEPFEIAQNAAKVAETVRQKAHATEKAEGKQDLVKDKVTVKTSTTGEPVIEQTGEA
                     SSYGRGFEGKKTANGEKFDKNDLTAAHPTLPLGTKATVTNLENGKSVDVRINDRGPYV
                     KGRDIDVSQGAAKELDMDKNGVAPVKIEAKVPPSDISEGGGSSPAVKETAKPKK"
     gene            3222..3431
                     /locus_tag="VMZ02_07315"
     CDS             3222..3431
                     /locus_tag="VMZ02_07315"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HUR71777.1"
                     /translation="MEEHIYKQVELTGSSHASMEAAVQNAITKAGKTLHNIHWFQVID
                     TRGYIADNKVDYWQVTIKLGFRLDD"
BASE COUNT          960 a         1087 c         1074 g          931 t
ORIGIN      
        1 gacgctcgag taatgcaaat gctcctgtag gtagatgggc aaaaaagtca gtatgatcag
       61 gcgcgacatg gaaagtaagc cggtggcaac cgacagtcct aaaaaggccg gattcttgat
      121 gatggtagcg acaccgcgaa attgcgcggc gatgcgctgg cgcggggcgg cgctatccct
      181 gaacactcct gccagagcgc gccagagcag aaaagcaaat aagactccag gaaacagttg
      241 cagctgcgtg gcggcttgcc acgacagatt ggccagcaga atgccgacta ggagcggcgt
      301 gatcgtatcg ctgatcgtcg caccggtgcc gtgaatcgaa agtgccgtcg cgcgacgttc
      361 cggaaattga ttggatagcg atgccgcggc agtcggatgc cacagggcgg tgccgaatcc
      421 gatcatcgcc gagccgagca acgcccagct aaaagccggc aatgccaaaa gaaaataggc
      481 agcgcccatg gcaatcagcg agagcgcgag aatcgtcgcc cggtggcgca ccatcgcgtc
      541 ggcaaccatg cccaagggca actgcccgag accgaccacc acctgctgcg ccgacgtcaa
      601 ggcgccgact tcgacgttgg acagattcag tcccgatttg atcgacggca acagcactgg
      661 aaaaaactgc tgaaaccaat gaatgacaaa atgaccgacg gtgagaacgc cgaggagcca
      721 gatgcgttcc ttgccgagcg cgccgatgga tttaaaaaaa tcttgtgcgg tattcatttc
      781 tactaacaag ccgatagcgc gatcgagccg ttacggagcg aaaaggaaag ccggtccgca
      841 tgaatcttcc atcaatagcg tagacgacga cgggttggaa ggattttacc taagcgactg
      901 cgcctagttt cttttccgtg cgagcatcag gcgcgcacgc attgcacacc gcaacggatg
      961 cagctatgga ctgatagatc tcgcattcgc gctaaggagc tctgcaacat gaagctcagt
     1021 atattcgcat acctcgcttt cgtgatcttc ctggcgattt accatgattc tccggcgcaa
     1081 atcgccgcct cgccgaccaa ggtggtgatg accatcggcg cgatcagcga acgagagggc
     1141 gtcgtttatg ttgcccagga tcaaggcttc tttcgcaagc atgggctcga tcttactctc
     1201 gtgcaggtgc gcaacggcgc ggtcggcatc gcggcgttga gctcgggaga atcgcaggtt
     1261 cactggggct cggtttcggc cgccaacctc ggcgccatcg ccgaaggaac cgacctggtt
     1321 tttctcgccg gctttatcaa taagctgacc ggcgcgtttg tcgtcaatcc caagatcaaa
     1381 agtccggcgg agttgaaggg taggaatatc ggcgtcaatg gtttaagcgg cggcgcatgg
     1441 atcttcgcca tgctgacgtt ggattactgg gggttggtgc ccgagcgcga caacatccaa
     1501 ttccgcgcgc tcggcgatca aggggtgatc gcgcaaggac ttgctgcagg cgccgtcgat
     1561 gcggccttcc tgggttacac cttcggcaaa gcactggaga gcaaaggctt tcgcattcta
     1621 gccgatgtcg agagactacc gatcccgtac caaggttcag gaattatggc gcggcgcagt
     1681 ttcgcgctgt cctcgccggc gattgttgaa aatgtactca gaggtctgct cgacagcctt
     1741 gcctttattc gcgatccagc gaacaaagcc gcggtgttga aaagcctcgc caaaggactg
     1801 cgcctcaagc gtgtcgaaga agcggaggaa ggctatcaaa tcatggtcgg cggactctat
     1861 gagaaaaaga tttatcccag cgtcgacggt atccgcaacg tcattcgctt gctcggcaaa
     1921 accaacgaaa agattcgtcg cttgaaagca gaggacctcg tcaacgatag ttcggtgaag
     1981 aagctcgaaa aagaaggccg gttttagcgc gctggcaatc tgctgtggtc cggaagtcat
     2041 caatgctcga cgctggccgc cggccactat aattatttgg ttcgcgtaat cgagtggtga
     2101 aatttgctaa gaaacgcttt tcttatgagt taaggcctta gaaaaaggga ggcaccgtcc
     2161 atcggaggta attggccttc attcatccac tcggttacgc gaagagccat tattttttag
     2221 gcttcgcggt ttcctttaca gccggtgaac ttccgccgcc ttcagatatg tcactgggag
     2281 gtaccttcgc ctcgattttt accggggcca cgccgttttt atccatatct aattctttgg
     2341 ctgcgccctg agaaacatca atatcccgtc ctttcacata aggaccgcgg tcgttaatgc
     2401 gcacgtctac ggatttgccg ttttccaaat tagtcactgt ggctttggtc cccagaggta
     2461 atgtcgggtg cgctgccgta agatcgtttt tgtcgaattt ctctccattg gcggtctttt
     2521 ttccttcgaa gccccgcccg tagctcgacg cttcgccagt ctgttcaatt accggctctc
     2581 ccgttgtcga tgtctttact gtcaccttgt ctttgaccag atcctgtttg ccctcggcct
     2641 tttcggtggc gtgcgccttt tgccgcacgg tttcggcaac ttttgcagcg ttttgcgcga
     2701 tctcaaacgg ctccatcgcc tgaatctcct tgtctcgatc gcacgccggg agagcaaaga
     2761 gcgaaaaaat gagccagatt accagtcgaa gcggtacttc attcatactg acctccacga
     2821 agctgggtac ttgatgaacc aaggaatatg ccaacaaaat tatctgcaaa actcacgtag
     2881 ctaacgctca tgtctcaaca gtgagacatc gggaatgatg caggcgtcac cttcgggact
     2941 caccgaggca aaacaaaaac accgctgagg tgccgggtcg gcaagaattt gtctctattt
     3001 ctttcaccaa agttatttca ccaagattta accgctaata aagttttaat gcgccctggt
     3061 catacttact ttccgcaagc gaactggcct gcacggtagc gtgcagctct caacaattaa
     3121 attttttata ttgactgacg attttgtcat gcttgaataa accaagtgac gcttgttggc
     3181 tgcgcaaagg ttgtttcgac gtaaactcaa gggaggcttc aatggaagag catatttata
     3241 aacaggtcga actcacaggc tcctcgcacg cttccatgga agcggcggtt caaaacgcca
     3301 tcaccaaggc cggcaagacg cttcataaca ttcactggtt tcaggtgatc gatacacgcg
     3361 gttatatcgc cgacaacaag gtcgactact ggcaggtaac gatcaaactg ggctttcgcc
     3421 tggacgacta aacttttctg tcgcggtgcg ctgcgatggt tgggtgtcct aggtttcatt
     3481 agttggcgct agcggtgtcg acaaccgagc gtgcgagcgc tgcgcgtcga tgagttttat
     3541 tagtccgcat gtggtccagg gaagtcgcct accacatcag acgctgacat tataaatccc
     3601 taaatcagag caatctcgcc gtagaacgct cctttcaaat gatttttggc gccggcgcga
     3661 atagcaatcg cgcatgatgc ccccctccac cgccggtgaa agttgccagt aatgcccccg
     3721 agccccttcc gttttcgtcg agagtcttgc caactggctg ctcgtaaaaa ctagaacgac
     3781 ggcaggatcc caggcaattg ccatgggata atgacctgat tccatcgatg ctttacaacc
     3841 cgaaaacctc agcgtacatt ttttctcgat gtcgcttgca aaaatgctta agcattcgcg
     3901 cggcggcggt ttcacaacat tccacaatca accattttat ctctgctgca ttcgtcagca
     3961 caattctcaa gcgaacctat gaacgatatc tttatcagtt atgacagtgc ggaccgggcg
     4021 acagcgcaga agtatgccga cgcgttagaa tc
//
LOCUS       DATLCH010000248         3222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8532801, whole genome shotgun sequence.
ACCESSION   DATLCH010000248 DATLCH010000000
VERSION     DATLCH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8532801"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VMZ02_07320"
     CDS             complement(<1..637)
                     /locus_tag="VMZ02_07320"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HUR71778.1"
                     /translation="MNIAADRRAFLGSIGALVILAWMTLWIWDRSPYARYLDHGSLGE
                     IGTASLLIPVMLYLVGWTLMTIAMMLPTTVPLLEIFHRLIAQRPERSQLMALLIAGYL
                     GVWAAFGIAAHLADWVLHELVERNPWLEANAYVIGAGTLLVAGAFQFSRLKYRCLDKC
                     RAPLGFVMSYWRGANNRRNALLLGINHGLFCVGCCWALMLLMFGVGVGNIGW"
     gene            complement(739..1341)
                     /locus_tag="VMZ02_07325"
     CDS             complement(739..1341)
                     /locus_tag="VMZ02_07325"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HUR71779.1"
                     /translation="MAYKLEGSLLEVCNCEVLCPCWVGVDPDNGTCDTALAHHFEKGE
                     IDGVDVSGLTMGFCAHVPGNILKGNWRVVVHIDERATKEQEQALLSVYTGKQGGPVAD
                     LVKLIGEVVAVERSPITFEVEEGKGTLRIGTVADCEMEPFRGPNGEVTTLRDSIFSTI
                     PGAPAYVARATKYKMNNPALKQVIDLKDHNAIQGKFIFQS"
     gene            complement(1460..2836)
                     /locus_tag="VMZ02_07330"
     CDS             complement(1460..2836)
                     /locus_tag="VMZ02_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HUR71780.1"
                     /translation="MASALSLSDAITEFSKTFTGQILQPSDPAYDEVRKVHNGLVDKR
                     PALITRCRGVADVIDAVNLGRKLNLEVAVRGGGHNVAGRSTTEGGLMIDLSLMKGIHV
                     NPKARAARAQGGLTWNEFNRETQLYGLATTGGVVSTTGIAGLTLGGGLGWLMSKHGLA
                     LDNLLSVDLVLSDGRILTASTQDNADLFWAVRGGGGNFGVATSFEYRLHPVGPIVTGG
                     LIAYPFSAAWDVLRFFRDVTASLPDEFSVFAGLIHAPDGSGTKLVALVVCHCGESGDA
                     ARGAQPIKKFGAPLMDVIGPMPYVAVNAMLDAAYPRGALNYWKSNFLASLSDEAIRTM
                     IDCFAKCPTPMGQLLLEHFHGAVTRVGPTETAFPHRAPGYNAVVISEWMQPKDSNACI
                     AWARDSFTAMQPFMGSGRYMNYLGDDETGDPAAAAYGPNYQRLQQVKANYDPENFFHV
                     NQNIQPSS"
BASE COUNT          756 a          971 c          832 g          663 t
ORIGIN      
        1 tccagccgat atttccgacg cccacgccaa acattagaag catcagtgcc caacagcagc
       61 cgacgcaaaa cagaccgtga ttaataccga gcaatagagc attgcggcga ttgttagcgc
      121 cgcgccagta gctcatgacg aagccgagag gagcgcggca tttgtccagg cagcgatatt
      181 tcaggcggct gaattgaaac gcgccagcga ccaacaaagt gcccgcgccg ataacatagg
      241 cgttcgcttc aagccatgga ttgcgctcga cgagttcatg caagacccaa tcagccaaat
      301 gcgccgcgat accgaacgct gcccacacac ccagataacc ggcgatcagt agagccatca
      361 gttgcgaccg ttccggtcgt tgcgcgatca agcgatggaa tatttccaat agtggaacgg
      421 tggttggcaa catcatggcg atcgtcatca gcgtccaacc gacgagataa agcattacag
      481 gtatgagcag cgaagctgtg ccgatctcgc ccaaactgcc gtgatcgaga taacgagcat
      541 aaggcgatcg atcccagatc caaagagtca tccacgctag tatgaccagt gcgccgatgg
      601 acccgagaaa agctctacga tcggcagcaa tgttcacaaa tactccggca caatgccgtt
      661 ccgatgtttg tcggcgtcgc tagactaaaa agtacgggcc cagcgacggc aatcttttcc
      721 caccaactca cccctgcctt agctttggaa aataaatttc ccctgaatcg cgttgtgatc
      781 cttgaggtcg atcacttgct tcaatgccgg attgttcatc ttgtacttgg tcgccctcgc
      841 cacgtatgcc ggcgcgccgg gaatggtcga gaatatcgaa tcgcgcagtg tcgttacttc
      901 gccgttcgga ccacggaaag gttccatttc gcagtcggcg acagtgccga tccgcagggt
      961 tcccttgcct tcttcgacct cgaaagtgat cggcgagcgc tccacggcaa cgacttcgcc
     1021 gatcaacttc accaggtccg cgaccggccc gccttgttta cccgtataaa cactcagcag
     1081 cgcttgttcc tgctccttgg tcgcgcgctc atcgatatga acgaccacgc gccagttacc
     1141 tttgagtata ttgccgggaa cgtgggcgca aaaacccatc gtaagacctg acacatcgac
     1201 accgtcgatt tcacctttct caaaatggtg cgccagcgcg gtgtcgcaag tcccattatc
     1261 cggatcgacg ccaacccagc acggacacag cacctcgcaa ttgcagacct ctaaaagact
     1321 cccttcgagt ttgtaggcca tgatcgtctc cctcctaaaa gtttttcggc gaacacttac
     1381 caaagcgcat gaagactata atccttttcc attcctcgct tgaagtgctt aggctagagc
     1441 cacaaaaagc caaccctgct caagatgacg gctgaatgtt ctggttcaca tgaaaaaagt
     1501 tttccggatc gtagttcgcc ttgacctgct ggagccgctg ataattcggc ccatacgcag
     1561 cagcggcggg gtcaccggtt tcgtcgtccc cgagatagtt catataacgg ccggagccca
     1621 tgaacggctg catcgcggta aaagagtcgc gcgcccaggc aatgcaagca ttgctgtctt
     1681 tcggctgcat ccattcagaa attacgaccg cattgtagcc cggcgcgcga tgcggaaatg
     1741 cggtttccgt cgggccgacg cgcgtaaccg caccgtgaaa atgttcgagg agcaattgac
     1801 ccatcggcgt cgggcacttt gcaaagcaat cgatcatcgt gcgaatcgcc tcgtcgctca
     1861 gactcgcgag gaaattcgac ttccaatagt tgagggcgcc gcggggatag gcggcatcga
     1921 gcatcgcgtt caccgcgacg tagggcattg gtccaatgac atccatcagg ggcgcgccaa
     1981 acttcttgat tggctgcgcg cctctcgcgg cgtcgccgga ctctccacag tggcaaacca
     2041 ccagagccac gagcttcgtt cccgatccat ctggcgcatg gatgaggccg gcgaataccg
     2101 agaactcgtc ggggagcgat gccgtgacgt cgcggaagaa ccgcagcaca tcccaagctg
     2161 cgctgaacgg gtaggcgatc agaccgcccg tgacgatggg cccgaccgga tgcaagcgat
     2221 attcgaacga agtagcgaca ccgaagttgc cgcctccgcc acgcacagcc cagaacaaat
     2281 cagcgttgtc ttgcgtgctc gccgtgagta ttctgccgtc gctcaaaacg agatcgaccg
     2341 aaagcagatt gtcgagagcg aggccatgct tgctcatcag ccaaccgagc cctccgccga
     2401 gcgtaaggcc agcgatgccc gtggtcgaaa ccacgcctcc ggtagtggcc agaccgtaga
     2461 gctgcgtttc acgattgaac tcgttccacg tgagaccgcc ctgcgcgcgc gcggcgcggg
     2521 ctttcggatt cacgtgaatg cctttcatta gcgaaagatc gatcattaac ccgccctcag
     2581 tggtcgagcg gcctgccaca ttgtgaccgc caccccgaac ggcgacttcg aggttcagct
     2641 tgcgacccag gtttacggca tcaataacgt ccgccacccc acgacagcgc gtgatcagcg
     2701 ccggccgttt gtccaccagg ccgttgtgga ctttcctgac ttcgtcatag gccggatcgc
     2761 tcggctgcag gatctgcccc gtaaacgttt tagaaaactc ggtgatcgcg tcactcaatg
     2821 ataatgctga tgccacgaat gtctcctcgt tggctaattg aagaaaaaag cgcgctatgc
     2881 attttgccgc cggcaacgca tcaagtgaac cgccgacttg gtgacatacg ctcgaacata
     2941 cttaccgcga cactccggaa attggctaaa agaaaattta atatccaccg cgtctcccag
     3001 cttgtcaatg ctccgcctac cgtaaaccga cctacggcaa ttcgcgcttc cggcatcagc
     3061 ggcctaatca atccaccatc gattatttgc cagtcaatag agaaacctga ttgtgcccat
     3121 cacgaaaaat aatttcggat aaagtccgat tctgtgaact gcgcgcgggt atttcggtta
     3181 ctgtttgcat ggcctcgctc tgcggttgac gctgtaaacc at
//
LOCUS       DATLCH010000249         6568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10776130, whole genome shotgun sequence.
ACCESSION   DATLCH010000249 DATLCH010000000
VERSION     DATLCH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10776130"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VMZ02_07335"
     CDS             complement(<1..1047)
                     /locus_tag="VMZ02_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71781.1"
                     /translation="MSKPAEQTDNVMDNIPFYDKWQLGEGIPIIKTFFVQDLKKVDLK
                     PWARTGGSGAFINMEGAEGATGAYVSEIPAGKKSNPLRHLYEDLHFVLKGRGATTIWN
                     DKGAKQTFEWHDGSLFALPLNCTYQHFNGSGDEPARFFSVNSAPIVFNLFHHEDFVFN
                     TPRDFSDRFDGDPEYFSGKGKAYPGRVWDTNFIADARSFALEDWVERGGGGKNVMLEM
                     ANGSMAAHISEFPVGTYKKAHRHGPGFNVIIIKGKGFSMFWRQGEEPKRYDWQDGSVF
                     TPPAMMWHQHFNTGATGARYLPPRLGGIKYSLGEGFGDITKVDKDVKAGGNQIEYYDE
                     NPNIRKMFEEELAKS"
     gene            1265..1480
                     /locus_tag="VMZ02_07340"
     CDS             1265..1480
                     /locus_tag="VMZ02_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HUR71782.1"
                     /translation="MATTIEAPMVGKVLKIEKQVGDHVEEDDVVIVMEAMKMEIPVVA
                     PTAGTIREVNVSPGQAVEAEQVLAVIE"
     gene            1528..1674
                     /locus_tag="VMZ02_07345"
     CDS             1528..1674
                     /locus_tag="VMZ02_07345"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HUR71783.1"
                     /translation="MDTWTFGWTLALIGMGGTMLVLWLMSLMILLIRKIFPYQADPSA
                     AKKG"
     gene            1684..2832
                     /locus_tag="VMZ02_07350"
     CDS             1684..2832
                     /locus_tag="VMZ02_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HUR71784.1"
                     /translation="MESALVSGVQGLFAGVVSVTPGHVIMLAIAVVLLYLGIKKGYEP
                     LLLVPIGFGAILVNIPLADMMGKDGFLRFFYDAGVLTEVFPLLIFIGIGAMTDFQPLL
                     QNPKIILLGAAGQFGIFLTLLLALALGFDKLDAVAVAVIGACDGPTAIYVSSKFAPHM
                     LGAVSVAAYSYMSLVPLIQPPIMRAFTTEKERKIVMGAAKLEPISKTTKIVFPIAVTI
                     FASILAPKGAPLIGTVMLGNLLRESGVVERLKSASENEIANIVTLLLGLCIGATMEAD
                     KFLKAQTLLILGLGFIAISLDTVVGLLFGKLMCLMTGGKINPLIGAAGISAFPMAARV
                     VQTEGQKYNKKNYLLMHAMSANAGGQIGSVIAAAVMLSVLQGMGIVTG"
     gene            2846..4399
                     /locus_tag="VMZ02_07355"
     CDS             2846..4399
                     /locus_tag="VMZ02_07355"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HUR71785.1"
                     /translation="MSKSRQELENKLAGLEQKIVAGDPKTTAKLHDQGNLTARERIEK
                     LLDPATFVEEFMLAETPCMDFGMSERKQPSDGVVTGFGKIDGRPVYVYSQDRSILAGS
                     VGAAHAEKIAYVIQSARKLGMPVIGLNDSAGARIQEGLSVTSAIGKIFFENSIASGCV
                     PQISAIMGPCIGVGSYSPALTDFILQVQNSGQMFITGPAVIKEVLGETVTMEELGGAK
                     LHSEVSGVTDLVAKNDEECLAQIRRLVGFLPANFEEKPPRKRTTDDPNRTLDNLESIV
                     PEDMKRAYNIVQVIKAICDDGDFFEMKAKFARNLVIGFGRLDGHPVGFVANQPMFLAG
                     SLDVDAADKAARFIRFCDAFNVPIITLMDVPGFFPGKQQEEKGIIRHGAKMLYAYAEA
                     TVPKITIVLRKGYGGAKQALCTREMGADQLFVWPGVELAVMGGGGAVNVLYKKEIDQS
                     SDPAKTRAEKLAEYQERFSGPFEALSKQFAHAAIRPAETRKRLIQCLEILAGKKEDRP
                     RKKHGLMPV"
     gene            complement(4386..>6568)
                     /locus_tag="VMZ02_07360"
     CDS             complement(4386..>6568)
                     /locus_tag="VMZ02_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HUR71786.1"
                     /translation="KDFVIGDFQLLGNWQRRALMFGYPLPNDSGAVQHTVIAALDVKW
                     IAQLAVETALPDGMTLTILDRNGTILTRVPDQAEWTGKHMPDAPLLELKQLRNQPTKE
                     IDGLDGVTRLYAFNTIGGQGERGQLHIIVGLSRSIAYREANYALLRSLFWIVVVTALT
                     SGVAWFVGSNSVVEFLKRRAIAEEARGRLAAIVDSSEDGIIGMTLDGVITNWNGGAEQ
                     IYGYDAATILGQNIALLTPAENRAEIPELMQFVNLGKGINRYESERICRDGRRIAVSA
                     SLSPIRDAQANVRGAATITRDITLLRKGEQQLLLHTRQLEALQSIAQGTAETLSLAEM
                     VPHSLEKLVALVPCDYSVAYFIDAEGVVNSYSANRAAETNSLHGGLAGDLSATAVQCT
                     GEWFVADVTVVPELTLLWAQHGIRSMAMLPMTRKDRCRTTLVLLYNESRSFGLEETQF
                     LKALARPIALGVENGQLYESSLSLIDGLADEIEERKKAEKQLADFNAMVVHDLRSPLS
                     NIVSMAESVKEGVFGPINDMQRNWLGKIETNCRTLIDQVSDFLDFARIDAGKLNLKGG
                     RLNLEAEIDEILAEHSIQAEKRNIQLTAKIGQQLPVLWADSRRIGQVLTNFMSNALKF
                     TDDGGIIEVAACRTDGSVVVSVRDSGVGIDAEEQKHIFQMYSQVSSSEKSQKRGSGIG
                     LVICKKIIEAHGGRIWVESGLGKGSSFYFSLPTRLDKDEWLTPA"
BASE COUNT         1506 a         1804 c         1739 g         1519 t
ORIGIN      
        1 ggacttggcc agctcttctt cgaacatttt tcggatgttt ggattctcgt cgtaatactc
       61 gatttggttt cccccagctt tgacgtcctt gtcaaccttg gtgatatcgc cgaagccttc
      121 gccaaggcta tatttgattc cgcccaaacg cggcggcaag tagcgtgcgc ccgtcgcgcc
      181 agtattgaaa tgctggtgcc acatcatcgc cggcggggta aaaacactgc cgtcctgcca
      241 atcataccgc ttgggctcct cgccctgacg ccagaacatc gagaagcctt tacctttgat
      301 aatgatgacg ttaaaaccgg gaccgtggcg gtgggctttt ttgtaggtac ccaccggaaa
      361 ttcggagata tgcgcagcca tggagccatt ggccatttcc aacatgacgt tcttgccgcc
      421 gccaccgcgc tcgacccaat cttccaacgc gaagctccgc gcgtcggcga tgaagttggt
      481 gtcccacacg cggccggggt aggctttacc tttgccgcta aaatattccg ggtcgccatc
      541 gaagcgatcg gaaaaatcgc gcggtgtgtt gaagacaaag tcctcatgat gaaacaagtt
      601 gaaaacaatc ggtgcgctgt tgactgagaa aaatcgcgcc ggctcgtcac cggaaccgtt
      661 gaagtgctga tatgtgcaat taagcggaag ggcgaacaaa ctgccgtcat gccactcaaa
      721 ggtctgcttg gcgcccttgt cgttccaaat ggtcgtcgcg ccccggcctt tgaggacaaa
      781 gtgcaggtcc tcatagaggt ggcgcaacgg atttgatttt ttgccggcag gaatctcgct
      841 tacataggcg ccagtggccc cctcggcacc ctccatattg atgaacgcgc cgctgccgcc
      901 ggtgcgtgcc cagggtttaa gatcgacttt cttgaggtct tggacgaaaa aggtcttgat
      961 gatcgggatc ccctcaccca actgccactt atcgtaaaag ggaatattat ccatcacgtt
     1021 atcggtttgt tctgctggtt tagacataac tttcctccgc gaaacttgca ggtttaatat
     1081 cgcatcgatt cagcgaaagc aaggtgactc gtgcctcata cgtcggtcga gaaaacgctt
     1141 gaaaacgcgt cgatgaatct ggctatagca ttgactttga agagcaaact ctactaaatg
     1201 gccactacgt aatcgatgcc tcagcatcgt taaaggactg cgacgagagt aaaggagagc
     1261 tcgcttggca acgacgatcg aagccccgat ggtgggtaaa gttctgaaaa tcgaaaaaca
     1321 agttggcgac cacgttgaag aggacgatgt ggtcattgtc atggaagcca tgaaaatgga
     1381 aattcccgtc gtcgctccca ccgccgggac gataagagaa gttaacgttt ctccgggcca
     1441 agccgtcgaa gccgagcagg tgctcgccgt catcgaataa ctgcagattt catccttctc
     1501 agtattctaa tccccaagga gattttcatg gacacctgga cgttcggctg gacgctggcg
     1561 ttgatcggta tgggcgggac catgttggtc ctctggctca tgagtctaat gattttgctc
     1621 attagaaaaa ttttcccgta tcaagccgac ccctcggcag cgaagaaagg ttaatatcag
     1681 ttaatggaat ccgcattagt ttccggcgtc caaggcttat ttgccggtgt cgtgagcgtc
     1741 accccgggcc acgtcatcat gctcgccatc gctgtggtgc tgctctatct gggcatcaaa
     1801 aaaggctacg aacctctgct gctggttccg atcggtttcg gcgcaatcct cgtcaacatt
     1861 ccgctcgccg acatgatggg taaggacggt tttctgcgat ttttttacga tgccggcgtc
     1921 ctgacagaag tgtttccatt actgattttt atcggcatcg gcgcgatgac cgatttccaa
     1981 ccgttgttgc agaatcctaa aattattcta ctcggcgccg ccggccagtt tggcattttc
     2041 ttgaccttgc ttctcgcgct ggctctggga ttcgacaagc tcgatgcagt cgcagtggct
     2101 gtcatcgggg catgcgatgg accaacggcg atttacgtca gctcgaaatt tgcaccgcac
     2161 atgctcggtg ctgtatctgt cgcggcttat tcgtatatgt ctttggtacc tttgatccag
     2221 ccgccgatca tgcgcgcttt cacgacggag aaggaacgga agatcgtcat gggcgcagct
     2281 aaattggaac cgatctccaa aactaccaaa atcgtctttc cgatcgcggt aaccattttc
     2341 gcctcgatct tagctcccaa aggcgcgccg ctcatcggta ccgtaatgct tggcaattta
     2401 ctgcgcgaaa gcggcgtggt ggagcggctt aagagcgcct cggaaaacga gatcgccaat
     2461 attgttacgt tgctcctcgg attgtgtatc ggcgccacaa tggaagcgga taaatttttg
     2521 aaggcgcaga cgctgttgat tttaggtctt ggctttatcg cgatcagttt ggacactgtc
     2581 gttggactgc tgttcggcaa gttaatgtgc ttgatgaccg gcggcaagat taatccgctg
     2641 atcggcgctg cgggcatatc ggcgtttccc atggctgccc gcgtggtcca aaccgaaggc
     2701 cagaaataca ataagaaaaa ctacctgttg atgcacgcca tgagcgccaa cgccggaggt
     2761 cagatcggtt cggtcattgc cgcggcagtt atgctttcgg tgttgcaagg aatgggaata
     2821 gtcactggat aattaggcgg cgcccatgtc gaagtcgaga caagagctgg aaaacaaact
     2881 cgctggtctg gaacagaaaa tcgttgcagg cgatcccaag accacggcga aactccatga
     2941 ccagggcaat ctcaccgctc gtgagcgcat cgagaaattg ttagacccag ccaccttcgt
     3001 cgaagaattt atgctagcgg aaacgccgtg catggacttc ggcatgtccg aacgcaagca
     3061 accatccgac ggggttgtca ccggttttgg taagatcgac gggcggccgg tttacgtcta
     3121 ttctcaagac cgctcgatcc tcgccggtag cgtcggcgca gcccacgcgg aaaaaatcgc
     3181 gtacgtgatt cagagtgccc gcaagctcgg catgccagtg attggcctca acgactcagc
     3241 cggcgcacgc atccaggaag ggctgagcgt tacttcggcc attggcaaaa tctttttcga
     3301 aaacagcatc gcctccggct gcgttccgca aatctccgcg atcatggggc cctgcatcgg
     3361 cgttggttcc tattcgcccg cgcttaccga cttcatttta caggtgcaga actccggtca
     3421 aatgttcatc accgggccgg ccgtgatcaa agaagtgttg ggtgaaaccg taaccatgga
     3481 agagctcggc ggcgccaagc ttcactcgga ggtctcaggc gttaccgacc tagtggcgaa
     3541 aaacgatgag gaatgcctgg cgcagattcg ccggctggtc ggtttcttac cggctaactt
     3601 cgaagagaaa ccgccgcgca aaaggactac cgacgatcct aaccgtactc tagacaatct
     3661 agaaagcatc gtgccagagg acatgaaacg cgcctacaac atcgtccagg taatcaaagc
     3721 tatctgtgac gatggcgatt ttttcgaaat gaaggccaaa tttgcgcgca atcttgtcat
     3781 cggatttggc cgtctcgatg gacatccggt gggcttcgtt gccaaccagc cgatgttttt
     3841 agccggcagc ttggatgtcg atgctgccga taaagcggcg cgttttattc gtttctgtga
     3901 tgctttcaac gtgccgatca ttaccttgat ggatgtaccc ggcttctttc cgggaaaaca
     3961 gcaggaagaa aagggcatta tccgccacgg cgccaaaatg ctctatgcct acgccgaagc
     4021 gaccgtgccg aaaatcacca tcgtgctaag aaaaggatac ggcggcgcaa aacaggcgct
     4081 gtgcacccgc gagatgggcg ccgatcagtt attcgtctgg ccgggagtcg aactcgcggt
     4141 gatgggcggg ggcggcgcgg tgaatgtgct gtataagaag gagatcgatc aatccagcga
     4201 tccggcaaaa actcgcgccg aaaaactcgc ggaataccaa gaacgcttca gcggtccgtt
     4261 tgaggccctc tcgaaacaat tcgcccatgc cgcaatccga ccggcggaaa cgcgcaagcg
     4321 gctgattcaa tgcctcgaaa ttttggctgg taagaaagaa gatcgaccgc gcaagaaaca
     4381 cggtctcatg ccggtgtaag ccattcgtcc ttgtcaagtc gcgtcggcag ggaaaaataa
     4441 aagctgctgc ctttgcccag gccactctcg acccagatac gcccgccatg ggcttcaatg
     4501 atttttttgc aaatcaccag cccgataccg ctgccgcgct tttgactttt ttcactgctc
     4561 gacacttgac tatacatttg aaaaatatgt ttctgctcct cagcgtcgat gccgacgccg
     4621 gaatcgcgca ctgaaacgac aaccgagccg tcggttcggc atgcggccac ttcgatgatg
     4681 ccgccatcgt cggtgaactt tagcgcattg ctcatgaaat tggtcagcac ctgtccaatc
     4741 cggcgcgaat cggcccaaag caccggcagt tgctggccaa tttttgcggt caattgaata
     4801 ttgcgctttt cagcttggat cgagtgttcc gcaaggatct catcgatttc cgcttctaaa
     4861 ttcagcctgc cgcctttaag attcaacttg ccggcatcga ttcgtgcgaa gtccaaaaag
     4921 tcgctcacct gatcgatgag agtgcggcaa ttggtttcga ttttgcccaa ccagttgcgt
     4981 tgcatatcgt taattggacc gaacactcct tctttgaccg actcggccat cgagactatg
     5041 ttcgaaagcg gcgaacgcag atcgtgaacc accatggcgt tgaaatcggc cagctgcttc
     5101 tcagccttct tccgctcttc aatttcatcc gccaatccat cgatgagact aagcgatgat
     5161 tcatagagtt ggccgttctc tactccaagc gcgatgggtc gcgccaacgc cttgaggaac
     5221 tgagtctcct ccaacccgaa cgagcgcgac tcattgtata acaacaccaa cgttgttcgg
     5281 cagcgatcct tgcgtgtcat cggtaacatc gccatcgacc tgatgccatg ctgggcccac
     5341 agtagcgtta gctccggcac caccgtaacg tcagcgacaa accattcgcc ggtacactgg
     5401 accgcggtcg cgcttaagtc accggccagc ccgccgtgca gtgaattagt ttcagcggcg
     5461 cggttcgccg agtaagaatt gaccaccccc tctgcatcga tgaaataagc gacactataa
     5521 tcgcacggca ccaatgctac gagtttctca agagagtgtg gaaccatttc tgccagcgaa
     5581 agcgtttcag ccgtgccttg agcgatcgac tgaagcgctt ccaattggcg cgtgtgcagg
     5641 agcagttgct gttcaccctt gcgcaatagt gtgatgtccc gggtaatcgt cgcggcgccg
     5701 cggacgtttg cctgcgcatc gcgaattggc gataacgagg cggaaaccgc aatgcgccgg
     5761 ccgtcccggc aaatccgttc gctttcgtaa cgattgatgc ccttgcccaa gttcacaaac
     5821 tgcatcagtt ctggaatctc agcgcgattc tccgctggtg tcagcagcgc gatattttga
     5881 ccgagaatgg tggcggcatc gtagccgtaa atttgttcgg cgccgccatt ccaattagtg
     5941 ataacaccgt cgagcgtcat gccgataatg ccatcttctg aggaatcgac gatggcggct
     6001 aatcgcccac gggcctcctc ggcgatggcg cgtcgtttta agaactcgac aaccgaattg
     6061 ctgccgacga accaggcaac cccgctggtc agcgccgtga cgacgacaat ccaaaacaaa
     6121 ctccgcagta gcgcatagtt cgcttcccgg tatgcgatac tccgggacaa accaacgata
     6181 atatgaagct ggccgcgctc gccttgtccg ccgatggtat tgaatgcata gaggcgagtg
     6241 actccatcta aaccatctat ttccttggtg ggttgattac gcagctgttt caattctaaa
     6301 agcggcgcat cgggcatatg cttgccggtc cattccgctt gatccgggac tcgcgtcaga
     6361 atcgttccgt tacgatcgag aatcgtcagc gtcataccat cgggcagggc ggtctcgaca
     6421 gcgagctgcg cgatccattt aacgtccagg gcggcaatga cggtgtgctg aacggcgccg
     6481 gaatcattcg gcaacggata tccaaacatc aacgcccggc gctgccaatt accgagcagt
     6541 tgaaaatccc caatgacgaa gtccttag
//
LOCUS       DATLCH010000250         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10026642, whole genome shotgun sequence.
ACCESSION   DATLCH010000250 DATLCH010000000
VERSION     DATLCH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10026642"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VMZ02_07365"
     CDS             <1..180
                     /locus_tag="VMZ02_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71787.1"
                     /translation="MLPYVAGFVGEERLVFATDYNHSDSKFPHTVEEVMERKDLTDGL
                     KAKIMGENAAKLYSL"
     gene            complement(390..1388)
                     /locus_tag="VMZ02_07370"
     CDS             complement(390..1388)
                     /locus_tag="VMZ02_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71788.1"
                     /translation="MTPEFAILIRLVSSFRLDPSYLISIGWIVLTFAANVSAQDKLRI
                     SPSSPGLASWPVHLAAKEGFFTREGLTSEIIVMRTNTGIAALVTGSIDFTTAGGSAMR
                     AAVNGAPLKMILNITKKADLWIVAQKNILRVEDLRGKMVGVGGNWGTQFYQVLEALKP
                     SGVDKDVQLVSTGDVANGFPTLQQGSMPAVALTPPYSILAKRMGYRDLVKTSDVIPVS
                     PTTGLVTTKEKLDKEAPKIRRTIRALFRAVDFARTRKNETVQFIMNQYKMDKDVSEAV
                     YDAIMETLNPTLSLSDQEVQVELNRIAEQTKTKITMKPSELADFSLTRQVAQEFGR"
     gene            complement(1415..>2083)
                     /locus_tag="VMZ02_07375"
     CDS             complement(1415..>2083)
                     /locus_tag="VMZ02_07375"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71789.1"
                     /translation="CKKGEARLEWIAQVPVIDIPSTVAEIEFARANGACGIFLRGMID
                     DKRLSNPAFFPIYAAAEKAALPVCVHASTGNFDWMEMFDGESGFMRFKAPVLSGFHAI
                     VHDGIPKKFPKLKFAFVEVRAQWIPYMAIEIARRFERADNSVARNLIRDNHVWVTCQT
                     DDDLPQVLKYSGEDNIIIGSDYGHNDTSSEIEALRTIKTKGEVEPRIIDKILYDNAKV
                     LYGL"
BASE COUNT          457 a          570 c          568 g          488 t
ORIGIN      
        1 atgctgccct acgtcgccgg tttcgtcggc gaagaacgtc tagtcttcgc caccgactac
       61 aaccacagcg attcgaaatt cccccacacc gtcgaagaag tgatggagcg caaagatttg
      121 accgacggct tgaaggcaaa aattatgggc gagaacgccg cgaagttgta tagcttataa
      181 ctttatccaa ctctgtgaat ccctaacttc cttgtcccta tagaggccgg ggcgcgctgg
      241 tcatttcgag ccggagcgag aaatcttccg tttgtatgtg gtcgaacatg ccagatctct
      301 cgctcacgct cgtgatgaca atatgcacga ttgccgaatt gcgagtcacg ccccaaacgc
      361 ggcaaggaac taagcgagtg ctctgacgtc taccttccga actcctgcgc tacttgccgc
      421 gtcagcgaga aatccgccag ctccgacggc ttcatcgtaa tcttcgtctt cgtctgctcg
      481 gcgattcgat tgagttcgac ctgcacttct tgatcgctta acgacagtgt cggattgagc
      541 gtttccatga tcgcgtcgta gacagcttcg gaaacatctt tgtccatctt gtattgattc
      601 atgatgaact gcacggtctc gttcttccga gtccgagcga aatcgaccgc gcggaatagc
      661 gcgcggatgg tccgacgaat cttcggcgcc tctttgtcga gcttttcctt cgtagtcacc
      721 aacccagtcg tgggcgacac cggaataaca tcgctggttt tcaccagatc gcgatagccc
      781 atgcgcttgg cgagaatgct atacggcggc gtcagcgcga ccgccggcat gcttccctgc
      841 tgcaaggtag ggaaaccgtt tgcgacatcg ccggtggaga ccagctgcac gtccttatcg
      901 acaccggacg gtttgagcgc ttcgaggact tgatagaatt gcgttcccca gttgccgccg
      961 acgccgacca ttttgccgcg cagatcttcg actcgtagaa tatttttctg cgcgacgatc
     1021 cacagatcgg cctttttggt gatgttgaga atcattttga gcggcgcgcc atttaccgcg
     1081 gcgcgcatgg ccgaaccgcc tgcggtggtg aaatcaatgg agcctgtcac caacgcggcg
     1141 atgccggtgt tggtgcgcat gacgatgatc tccgaggtca acccttctcg ggtgaagaat
     1201 ccttccttgg ccgccaaatg caccggccag gaagcaagac cgggtgagga tggagagatg
     1261 cgcagtttat cttgcgcgga aacgttagcg gcaaaagtga gaacgatcca accgatggaa
     1321 attaagtagg aaggatcaag gcggaaggat gaaacgagcc gtattaatat ggcaaactct
     1381 ggagtcatcc ttgatccttc atcctttggc ctttttaaag tccataaagc actttggcat
     1441 tatcgtaaag aatcttgtcg atgatgcgcg gttcgacttc gcccttggtc ttgatcgtgc
     1501 gcaaagcttc gatctcagac gaggtgtcgt tgtggccgta gtcggaaccg ataatgatgt
     1561 tatcctcgcc ggaatacttc agcacctgcg gcaggtcgtc gtcggtttgg caagtcaccc
     1621 agacatggtt gtcgcggatc aagttgcgcg ctactgaatt gtcggcgcgc tcgaaacgcc
     1681 gggctatctc gatggccatg tagggtatcc actgggcgcg cacttcgaca aacgcaaatt
     1741 ttaatttggg aaattttttc ggaataccat cgtggacaat tgcgtggaaa cctgacagca
     1801 ccggcgcttt gaaacgcatg aagccgctct cgccgtcgaa catctccatc caatcgaagt
     1861 tgccggtcga tgcatgcacg caaaccggca gcgcggcttt ttccgcggcg gcgtaaatgg
     1921 gaaagaaggc ggggttgctc aagcgcttat cgtcgatcat gccgcgcaag aaaatgccgc
     1981 aggcgccgtt ggccctggca aattcgatct ccgccaccgt gctcggaatg tcgataaccg
     2041 gaacctgggc aatccattcg agacgggctt cgcccttctt gca
//
LOCUS       DATLCH010000251         4665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10778510, whole genome shotgun sequence.
ACCESSION   DATLCH010000251 DATLCH010000000
VERSION     DATLCH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10778510"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VMZ02_07380"
     CDS             <1..500
                     /locus_tag="VMZ02_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71790.1"
                     /translation="GPITNLNITPVATPAEAVRGVDIVLTATNSSVPVFDGNLLEPGQ
                     HVTTIVGSNVGLVKGGFTAAKRREIDDATLSKSHVHGIVSIQQAVQDEQADIFDPVNR
                     GVIKWEQLIEIGSILAGNSEGRTRLDQITFYKNNAGQGVADVALGAKVLENVRAKKAG
                     QILDY"
     gene            complement(725..2164)
                     /locus_tag="VMZ02_07385"
     CDS             complement(725..2164)
                     /locus_tag="VMZ02_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71791.1"
                     /translation="MAQTTLTPNLQRLALTNVCLGQFLSALDSRSVNVALPTISLHFD
                     TSMAAVQWIPLAYQLTIVGLVLSMARLGDKLGRKGLYTLGFIMLGIGSAACGLSPYLW
                     LIVCFRALEGIGGALVLANGRAIASALYAKEGRGRALGMMSMSFHLGYITGPSVGGFL
                     VDTIGWRWIFFMNLPVALAAATMAWKILPETVIEKRDYSIDPIGMVTLLMTVVAMIVG
                     LQQVAKNGFAWPALLAGVVAVASLALLLYFERRDHSPLLDLSLFKVRLLTAGLLSHFF
                     VSLSHTSTFFLLPFYLQGIAGYTPTQVGFTVICFSLVIVVLAPIGGWLADRLGSRMLC
                     TVGSALTMLSMIGFSRLDGSAGQIGVVIPLMLLGLGWSLFQSPNLSGMFNAVEPRYVG
                     AVSGLSLTSANIANAMGVAVGSVLFMRWLNYYGLASGSVPPYTQWGGNAEIYVKAFQG
                     SWTIIACLAGIAIITSALRGVDRRQRSDG"
     gene            2529..3338
                     /locus_tag="VMZ02_07390"
     CDS             2529..3338
                     /locus_tag="VMZ02_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HUR71792.1"
                     /translation="MSKIAIAQMQSGTAKAENLKKALKLIGEAKSQGAELIAFPEFLM
                     AFSPSSQSAAELTAVAEAADGPFVAALRESAREAKLGVLATIYETSPVTHRVYDSAVW
                     IDAAGTILSVYRKLHLYDAFGFKESDKFYPGADIAPLISFGDAHCATMICYDLRFPEL
                     ARLLTLEGANILFAPSGWVQGDLKVDHWQTMIKARALENGCFVIAPNQVGNIYTGHSL
                     AVDPLGRTSVDLQEEEGLALVDVDFGLVKEVREKLPLLQNRRTDVYDKHRR"
     gene            3530..4108
                     /gene="pdxH"
                     /locus_tag="VMZ02_07395"
     CDS             3530..4108
                     /gene="pdxH"
                     /locus_tag="VMZ02_07395"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HUR71793.1"
                     /translation="MSHNPIQLFTDLLTQAEKLGIELPNAFALATSGDNQKNTVRMVL
                     LKDIDEHGLVFYTNLQSRKARQMTETPHASACFWWPQLKRQVRFEGRVELVSEQEADD
                     YFASRPRGSQIAAWASPQSSELASRQQLLDAVAAMTEKFKDQRVPRPPYWSGYRLRPE
                     RIEFWQEQPDRLHERDVYTRQTDGWKFALLAP"
     gene            4183..>4665
                     /locus_tag="VMZ02_07400"
     CDS             4183..>4665
                     /locus_tag="VMZ02_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HUR71794.1"
                     /translation="MNLPHLESSLNRLLTTASRATASILERALSGHDLSIDETTTLFE
                     ADGADLLAMLAAADQLRAQTVGDVVSYVINRNINFTNVCVKQCGFCAFSRGHLAEEGY
                     FLPIEEIIRRATEARDLGASEVCIQAGLAPGMDGWHYVKLCRAVKEALPALHIHGFSP
                     E"
BASE COUNT         1130 a         1309 c         1303 g          923 t
ORIGIN      
        1 tgggaccgat caccaatttg aatatcacgc cggtggcgac acccgccgaa gcagtgcgcg
       61 gcgtcgatat tgtgctgaca gcgaccaact cgagcgtgcc ggtgttcgat ggcaacctat
      121 tggaaccggg gcagcatgtc acgacgatcg tcggcagcaa cgttggcctg gtcaaaggcg
      181 gcttcaccgc cgccaagcgg cgcgagatcg acgacgcaac attatcgaaa agtcatgtcc
      241 acgggatcgt gtcgattcag caggccgtgc aggacgagca agcggatatt ttcgacccag
      301 tcaatcgcgg cgtcatcaag tgggaacagc taatcgaaat aggttccatc ctcgccggca
      361 acagcgaagg ccgcacccgc ctcgaccaga tcaccttcta caaaaacaac gctgggcaag
      421 gcgtcgccga cgtcgcgctg ggagcgaagg tgttggagaa tgttagggcc aagaaggccg
      481 gacagatact ggactattaa gtcgccgttg gttttttaat tacgcgaggt cgcgcacatt
      541 gacgatttca cctggttgaa actggaaatg gccgagtatg acttccggaa cttcagcgaa
      601 gaattccgca tttaccgcgc actggctaat gaatcaaacg ccataaacaa ttaagcaact
      661 ctggaacgac tcctgccccg aaatactcgc ttccatttac agcattaaaa gtgtccggaa
      721 gcgatcaccc gtcgctgcgc tgccgccgat ccacgccgcg cagcgctgaa gtgatgatgg
      781 cgatccccgc taagcaggcg ataatcgtcc acgaaccctg aaacgccttg acgtagattt
      841 cggcgttccc gccccactgc gtgtatggcg ggacgctgcc gctcgcgagg ccgtaataat
      901 ttaaccagcg catgaacagc acgctgccga ccgccacgcc catggcgttg gcgatgttgg
      961 cggaggtcaa cgacaggccg ctcaccgcgc cgacgtagcg cggttcgact gcattgaaca
     1021 tgccgctcaa gttgggcgac tggaacagcg accagcctaa ccctaacagc atgagcggga
     1081 tcaccacacc gatttggccg gcggagccat ccaagcgtga gaatccgatc atgctcagca
     1141 tggtgagtgc agagccgaca gtgcaaagca tgcgcgagcc aagccggtcg gcgagccagc
     1201 cgccgatcgg agcgagaact acgataacca gagaaaagca gatcacggta aaaccgacct
     1261 gcgtcggtgt gtagccggca atcccctgga gataaaacgg caacaagaaa aacgtcgagg
     1321 tgtgcgacag cgacacgaag aagtggctca gcagtccagc ggtgagcaag cgaactttaa
     1381 ataacgagag atcgagcagc ggtgagtggt cgcggcgctc aaaataaagc agcaacgcga
     1441 gcgacgcaac agccacgacc cccgcaagca aagccggcca ggcaaaaccg ttcttggcga
     1501 cctgctgcaa gccgacaatc attgcgacca cagtcatgag cagagtcacc atgccgatgg
     1561 gatcgatcga ataatcgcgc ttctcgatca ccgtctcggg gagaatcttc cacgccatgg
     1621 tcgccgcggc gagagccacc ggcaagttca tgaaaaaaat ccagcgccag ccgatggtgt
     1681 cgaccaagaa gccgcccacc gacggcccgg tgatgtagcc cagatggaac gacatcgaca
     1741 tcatgccaag ggcgcggccg cgcccttctt tggcatacag tgccgaagcg atggctcggc
     1801 cgttggccaa taccagcgca ccgccgatac cttcgagcgc gcgaaaacag acgatcagcc
     1861 acaggtacgg actcaggccg caggccgccg aaccgatgcc gagcatgata aagcccaagg
     1921 tatagagtcc cttgcgcccg agcttgtcgc ccaagcgtgc catgctcaag accagcccta
     1981 cgatggtcaa ctgataagcc aacgggatcc actgcaccgc cgccattgaa gtatcgaaat
     2041 gcagcgagat agtcggcaag gcgacgttca ccgagcgcga atcaagggcg ctgagaaatt
     2101 gacccaggca aacattggtg agcgcgagtc gctgtagatt cggagtcagg gttgtttgcg
     2161 ccatttcgtt agtatgcgga gcgtgagttt ctcgattatt aaaatggttc catttcacgg
     2221 tagtactgca ataccgaatc aagtagattt ctcgcttcgc cagactgtgt ggtaattgtc
     2281 atttcgacgc gaagcggaga attgtttttc tatgtgtaca aaatgcaaga tctctcacgc
     2341 cgttcgagat gacagatcag agagacgctg agttttcaca cagtctgtcg ctcgaaatga
     2401 caaaatccgt cacccggcac gaagagaggg atctttccga gtggaaccag cacccgttca
     2461 acgattgacg cagttaacct acttggatag cttgactggc aaagataatc caggctgaga
     2521 gataaatcat gagcaagatc gccatcgccc aaatgcaatc cggcaccgcc aaggctgaaa
     2581 atctcaagaa ggcgttgaaa ctgatcggcg aagccaaaag ccaaggcgcc gaactgatcg
     2641 cttttccgga atttctcatg gcgttttcgc cgtcgagcca gagcgcggcg gaattgaccg
     2701 cagtggccga ggccgccgac gggccatttg tcgcggcgct gcgtgaatct gccagggaag
     2761 cgaagctcgg tgttttggcg acgatttatg aaaccagtcc ggtaactcat cgagtttacg
     2821 atagcgccgt ctggatcgac gccgccggca caatcctgtc ggtttaccgc aagcttcatc
     2881 tctacgacgc cttcggcttc aaagagtcgg acaagtttta tcctggtgcc gacattgcgc
     2941 cgctgatcag cttcggcgac gcgcactgcg cgacgatgat ttgttacgac ctgcgctttc
     3001 cggagttggc aaggctgctc accctcgagg gcgcgaatat tttgtttgcg ccgtcaggct
     3061 gggttcaagg cgacctcaaa gtcgaccact ggcagaccat gatcaaagcg cgcgccctgg
     3121 aaaacggctg cttcgtgata gcaccgaacc aagtcggtaa tatctacacc ggccacagcc
     3181 tggcagtaga cccgcttggg cgtacctcgg tcgatctgca agaagaagaa ggtctggcgc
     3241 tagtcgatgt cgattttggg ctcgttaaag aagtccgcga gaagcttccg ttgctacaaa
     3301 atcgccgcac ggatgtttac gataagcacc gacggtagtc gttttcttcc ggtaagatta
     3361 aaatccctcg ccgcttcatt atgaccgata ggacttgtat taaattcgtc atgccggcgg
     3421 aagccggcat catcatcgtt cgttttaagt cccaaatcag aagttggatt ccggcgtgcg
     3481 ccggaatgac ggactagaat aaaaaatcaa ggaattgcct gcaccggtta tgagccacaa
     3541 cccaatacaa ctatttacag atctgttaac acaggccgag aaacttggca tcgagctccc
     3601 gaacgcattc gcgttggcta cttcgggcga taatcagaag aacaccgtgc gtatggtttt
     3661 gttaaaagac atcgatgaac atgggttggt cttttatacg aacctgcaga gccgcaaagc
     3721 gcgacagatg accgaaaccc cgcatgcatc ggcgtgtttc tggtggccgc aactgaagcg
     3781 ccaggtccgc ttcgaaggcc gggtggaact ggtcagcgaa caggaagccg atgactactt
     3841 tgccagccgg ccgcgcggca gtcagatcgc cgcttgggca tcgccgcaga gcagcgagtt
     3901 ggcttcgcgc caacaactgc tcgacgcggt ggcagccatg accgaaaagt tcaaagatca
     3961 gcgggtgccg cggccgccgt attggtccgg ttaccggctg cggcccgaac gcatagagtt
     4021 ctggcaagaa caacccgatc gtttgcacga gcgtgatgtc tatacgcgcc aaaccgacgg
     4081 ctggaaattt gcgctgttgg cgccttaacc tgagggttac gccatagctc ggttcccgac
     4141 ttcgtcgttg agtttcccca agtgaaaggc tagagtcgcg ccatgaattt gccgcatctt
     4201 gaatcgtctc ttaatcggct gctcacgact gcatcgcgcg caactgcgtc gattctcgag
     4261 cgcgcgcttt ccgggcacga tcttagtatc gatgagacga caacgctttt tgaagccgat
     4321 ggtgccgatc ttttagcaat gctcgcggcc gccgatcaac tgcgcgcgca aaccgtcggc
     4381 gatgtggtca gctatgtcat taatcgcaac attaatttta ctaacgtgtg cgtcaagcaa
     4441 tgtggcttct gtgcctttag ccgcggccat ctcgccgaag aaggctattt tttgccgatc
     4501 gaagaaatta tccggcgtgc cacggaagcg cgcgatctag gtgccagcga agtttgcatt
     4561 caggccggac tcgctcccgg tatggacggt tggcactatg tcaaactttg tcgcgccgtc
     4621 aaagaagctt tgcccgcgct gcatattcac ggcttttctc ccgag
//
LOCUS       DATLCH010000252         3468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2171464, whole genome shotgun sequence.
ACCESSION   DATLCH010000252 DATLCH010000000
VERSION     DATLCH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2171464"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VMZ02_07405"
     CDS             complement(<1..453)
                     /locus_tag="VMZ02_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HUR71795.1"
                     /translation="MITTVIFDLDGLLADTEPLHCQAYQLALREHGVHLTETDYGEHW
                     VRCGRGIHEWIDEHRHELDPGALRVRKAGHYQELLRSSLRPMNGAEALLNRLSGKMRM
                     GLASSSYRDAVDGVIYGLGISQFFEAVVSGLDVARVKPAPDIFLETAIR"
     gene            complement(593..1108)
                     /locus_tag="VMZ02_07410"
     CDS             complement(593..1108)
                     /locus_tag="VMZ02_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71796.1"
                     /translation="MNKVTGFIFGGAALATIALVSAPMAAAPQGGGASSGPPSVHRGP
                     MGGRFGGSAPLISIALKHKTELNLGTDQVANLEKIKSHYESQVTPLRQQVQAIEKEIA
                     SLAQQSPANLIQIKSKLQEAEKLRSELRYLQIEAIENGRTVLTPQQRDQLKTLVSSRQ
                     ENFRQMHKQSS"
     gene            1339..2292
                     /locus_tag="VMZ02_07415"
     CDS             1339..2292
                     /locus_tag="VMZ02_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71797.1"
                     /translation="MARTLSSRPHFAYLHHISLPCRNLAESKRFYIEVLGGELYHDTP
                     GFAEVMLAGMIVGLSEQSEGWTGWDAEYPHYGFNMESADFALAQSWLKGCGVPTHGWT
                     RNYKTALLYFRDPSGNLLELYCESGFGEVANLALGPRQGGVPLPLGALNYLWNGKVAH
                     SKLPRPRLASFAHLSVPAQNIEQAKRFFVEVMGGEPLATSDPETFTEVRVAGAIVGLS
                     TRSGVWTGRTAEYPHYAFYADGDNFLPLIDLLKRNGVTTPGPWTRDGKKGLMYFRDPS
                     GNLFEIYCGKDIPQAAQFPRGIKQGGSYATDYSKLFYDWQG"
     gene            2944..3405
                     /locus_tag="VMZ02_07420"
     CDS             2944..3405
                     /locus_tag="VMZ02_07420"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUR71798.1"
                     /translation="MEIENRLKALGIELPKVPTPAANYIGYVRVGNLLFIGGNIGRIN
                     GAIKYRGKVGAEVTLEQAYDAARNCALNHLAIMKAALGDLDRVERIVKVLGYVNVAPG
                     FIDMPKVVNGESDLLVQLFGESGRHTRAAIGVASLSNDAPVENEVTVQVKD"
BASE COUNT          742 a          899 c         1015 g          812 t
ORIGIN      
        1 acgaatggcg gtttcgagaa aaatgtccgg cgccggctta actcgggcaa cgtccagacc
       61 acttacaacg gcttcgaaga actgggaaat cccaagaccg taaatgacgc catcgacggc
      121 gtcgcgatag gaagaggaag ccagacccat gcgcatcttg ccggacaatc tatttaacag
      181 cgcttcggca ccgttcatgg gacgaagcga ggagcgcagc aactcctggt agtgacccgc
      241 cttgcggaca cggagcgcgc ccgggtcgag ttcatgacga tgctcgtcga tccattcatg
      301 tattccccgg ccgcagcgga cccagtgctc gccgtagtcc gtttcggtga gatgaactcc
      361 gtgttcacgc aatgccagct gataagcctg acaatggagc ggttcggtgt cggctagcag
      421 gccatcgaga tcgaaaataa ccgtggtgat catctttgtc gctgtggata attttactga
      481 agtttcgctg agattgcatc agctggcatg aaacgctggt ggtactctaa aaaaaatgcg
      541 gccaagcttc gctaaacttg gccgcatttt gacgatatcc gttgatcagc ttttacgagg
      601 attgtttatg catctggcgg aaattctcct ggcgcgagct caccaaggtc tttagttggt
      661 cgcgctgttg aggagtgagc accgtcctgc cgttctcgat ggcttcgatt tgcaagtacc
      721 gtagttctga gcggagtttc tccgcttcct ggagtttcga cttgatctgg atcaagttgg
      781 ccggcgactg ctgggcaagg ctggcgattt ccttttcgat ggcttggacc tgctggcgca
      841 gcggggttac ctgactttcg tagtggcttt taatcttttc caggttcgcg acctgatcgg
      901 tgcccaggtt taactcagtc ttatgtttga gcgcgatgct aatcaacggc gcgctgccgc
      961 caaacctgcc ccccataggg ccgcggtgta ccgagggtgg gccactggag gcgccgcctc
     1021 cctgcggggc tgccgccatt ggggcggaga ccagcgcgat tgtcgccagc gctgcaccgc
     1081 cgaatataaa accagtcact ttgttcatat accctccttc agggtgtgag attttgctag
     1141 attagacgat gcgcatcgac aaaggtttac caagctgcca agaagtagtc gcggctggca
     1201 ggccaaacac gtcgtgcgac agatccgact ggcgttaggc aatatatttc cgaccggaag
     1261 gaaatacttg ttggcgtcga acgtatggtg taatagtccg ttcagctaaa cactaggaga
     1321 cgaaccttgg aaactcaggt ggctcgcacc ctttctagcc gtccgcattt cgcctactta
     1381 catcatataa gtttgccctg ccgcaatctc gccgaatcca aacgcttcta catcgaagta
     1441 ttgggcggcg agctgtatca cgacacgccc ggctttgccg aggtgatgct ggcaggcatg
     1501 attgttggtc tgtcggaaca gtcggaaggt tggaccggct gggatgccga atatccgcac
     1561 tacggtttca atatggagtc ggcggatttc gcgctagcgc aatcgtggct caagggctgc
     1621 ggcgtgccca cccatggctg gacgcgcaat tataaaaccg cgctgctgta ttttcgcgac
     1681 ccgtcgggaa acttgctcga actgtactgc gaaagcggtt tcggtgaggt cgcgaacttg
     1741 gccttggggc cacggcaggg tggagtgcca ctgccgctcg gtgccctcaa ctatctctgg
     1801 aacggcaagg ttgcgcattc gaaattgcct cggccgcgct tggccagctt cgcgcatttg
     1861 agcgtgccgg cgcaaaatat cgaacaggcg aagcgttttt ttgtcgaagt catgggcggc
     1921 gagccgttgg ccaccagcga ccccgaaact tttaccgaag tgcgcgtcgc cggagcgatc
     1981 gtcggactat ccacgcggag tggagtgtgg acaggacgca cggccgagta cccgcattac
     2041 gcgttttatg ccgatgggga taattttttg ccgctgatcg atttgctcaa gcgcaatggc
     2101 gtgacgaccc cgggaccttg gacacgcgac ggcaagaagg ggctcatgta ttttcgcgat
     2161 ccctcgggaa atttgtttga gatttactgt ggcaaggaca taccccaggc cgcgcagttt
     2221 ccccgcggca tcaaacaagg cggcagctat gcaaccgatt actcaaagct gttttacgat
     2281 tggcagggct gaggtgtttc tcgcctgaga atcgaccgct gatttggaga ttagcgatct
     2341 acggagcata ttgttttcaa aataagccta aggtgtggct ggccgatgtt gagtttgaag
     2401 taccggcgag tcggtcgatg cctggctccg gccttagata acgacaaaac gtctggcaag
     2461 atcggtcgga cgacctgcgc tccgttgaac tcgttgccgg tatcggctta acgcgtcggc
     2521 cgggcgtgaa tcgttgtccc ggtacgatgc gacgatatcc tccaggagat cgatcaagga
     2581 cttaggttgg ttgccgcatg gactctgaat atcgctcata agctcacctc agcgcaaaac
     2641 tcgtgcacat tttgacctga tgagtttgct cttttgagac gattcttcct gaccgattgc
     2701 ggaactctgg atgacgcaga gtcggattag tgggtttggg tagtcgagcg ttgaatttct
     2761 ggcgcacaac tctggttccg cctcgactat ttttgttggc gcgctcaaat gctgcggagg
     2821 atttgcccac tgctgtaaag aatccaagtt attctcgcgg ttggagaaaa atgcgggagt
     2881 actgggttag attattttct tgcctgatgg tattgcggat ttaggcaaaa cgcggaggct
     2941 tccatggaaa tcgaaaatcg gctcaaagct ctgggcatcg aactgccgaa ggtaccgaca
     3001 ccggcggcca attacatcgg ctatgtgcgg gtcggtaatc tcctgttcat cggcggcaac
     3061 atcggccgga tcaacggcgc aatcaaatac cgaggtaaag taggggccga ggttaccctt
     3121 gaacaggctt atgacgcggc acgcaattgc gcgctcaatc atctagcaat catgaaggca
     3181 gcactgggcg atctcgacag ggtcgagcgc atcgtcaaag tcctgggcta tgtcaacgtc
     3241 gccccgggat ttatcgatat gccgaaggtc gtcaacgggg agtctgatct gctggttcag
     3301 ctattcggtg agagcggccg gcacactcga gcggcgatcg gcgtggcctc cttgagtaat
     3361 gatgcgccgg tggaaaacga agtgacggtt caagtcaagg actgaccgcg agtcaacagc
     3421 tggttagctg cgcaactacg acgcaaattg ccgccgctat ttttgcaa
//
LOCUS       DATLCH010000253        11449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2921059, whole genome shotgun sequence.
ACCESSION   DATLCH010000253 DATLCH010000000
VERSION     DATLCH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2921059"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..421
                     /locus_tag="VMZ02_07425"
     CDS             80..421
                     /locus_tag="VMZ02_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71799.1"
                     /translation="MTRIAVGYSGISVDQLVIWIARDAGIFAKNNLDVQAVYFSGGTI
                     SVTAMIAGDTPLIQASGPGIVSDEKKLPAKQYPSAEGIKTILDELALKEPKASAAQAE
                     RFYRCALCRGI"
     gene            complement(538..3672)
                     /locus_tag="VMZ02_07430"
     CDS             complement(538..3672)
                     /locus_tag="VMZ02_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71800.1"
                     /translation="MRTREKLLIAVVVAALILGSIIVYSMQGNEIEANYTRWLFGRNL
                     RQFISSPIAFVFGNSESGESSSVWFYAVLGGFGLVVMVFVLKMFRDGQIMDLKERVEA
                     LDAAKQEAEHMLQDAVWKGKTARQAKDSVTRDLEDSIERMETMILELTAKEQALKARD
                     DELRAIKSSSSTPRPILVNADAGDQALRAETMRLAEALHARDGEIKELRQQLSGKARL
                     WENQLQTKEDLLKRRAAELEIARSETNELSAQVKELEAASRRAEELLQKELQNKKEVL
                     EASDAANRNIEKRLQETIRSLESQAGDRERLLKSHESELAALNTELHLSALAKEQAES
                     QLAAVRADLEKEHDARESTVKELGSRLGNNIRDLQNQIEERDMLVQARDGEIHSLKAE
                     VHALSAKFDEAQRVSQQAETALNAALSKGQSISDEKAATVRALEERHSKSLRQLESQL
                     GEKEQVLQVREGEIQSLTLTVQSLTKKLDEEVTARANLEKSLTQELRKEKEIRQAKES
                     GNKELEARYTAELNVLQQQLTERDGLLQTRGTEIEALQTQLASLAEQLSKVGSAKERA
                     AGLLQEKLRKEKAILSASDSALKELEANFGSKISALEQQLAERQKLVGSRDAEVTALR
                     AELIAVQQKASEWSAAKRQAEEMLEEAFKEKASLLSTKNSAVQEMQAELLAKQQALEA
                     QLKDREKNLRNRELELQIAKRQLTEMDAISDQTARKFQADAKAKDHLLRARESALKEL
                     EKRSADRLRALETQLAEKQGLVDSRRGEVDSLRSKVKDLTGQLDLLSNTKEESVRALD
                     RELRQHKEALQAKEAALAELQNQFKGQIQALEAKLSEQQNLMTTREGEVDALMNKVRE
                     ISDKYSSLVSEKERSDRVLQEELREKTALLQAKESSVDDVEERLSGKLEFLERQLAEK
                     QNLLESGSEEMTAMRQQIAVLHEKLQETEVAKIHTERLLEEARAQGPHLPAVIRPDDD
                     ITAGDDTNGLDTLLSEREELLKARDSLIQNLMAELKEKKTQLARHEIEVWQGIERRGV
                     WKHRLSKVGIRLKD"
     gene            complement(3919..4989)
                     /locus_tag="VMZ02_07435"
     CDS             complement(3919..4989)
                     /locus_tag="VMZ02_07435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HUR71801.1"
                     /translation="MATPAQPVNLFEFEDAAKSLLAKEEYDYIAGGATDEISVDRNRR
                     AYASWAFRPRVLRDVSKLDLSTTVLGSRVELPVLIAPCGGHKRAHPEGEIATYRAAAS
                     CGTVLAVSANANTSFEDLAKAANGTLWVQLYPFRDKSMTQNWLDRAKANGYKAIVVTL
                     DSQWPPKRERNIRNRYQRTRGANYPAAGEHPDAPRPAGSGAGSDPAATWKDLEWIKAA
                     TSLPVVAKGIMTGEDVELCADVGADGVIVSNHGGRHLDNTLATVEVLSEAVAAAKGKM
                     EVLLDGGIRRGADVVKAIALGAKAVFIGRPLFWGLAVGGEQGVVRVLNLLREEIEITM
                     AKCGRPTIASIDDSTIVKAPPL"
     gene            complement(5028..5279)
                     /locus_tag="VMZ02_07440"
     CDS             complement(5028..5279)
                     /locus_tag="VMZ02_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71802.1"
                     /translation="MAEESIEAKLARLEAENQALKKQVDRKPGELRLKVSEKGGLSIY
                     GLGRFPVTLYKEQWNRLLEHADEIREFLKNNDQQLKAKQ"
     gene            5454..6539
                     /locus_tag="VMZ02_07445"
     CDS             5454..6539
                     /locus_tag="VMZ02_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71803.1"
                     /translation="MSANLGTRIIDGDGHILEDNAGIIAHMEGAYREIAGRKGTCFPP
                     LDHLHSGRAVETPPQRDGRAPVGPAGWLDFLDDIGIEWTVMYPTLALSYGKIVSRDYA
                     VAVCKAYNDWMSETYLKYNPRFKGMAIIPMQDPEEAAKELRRVVTELGMLGGMMPSNG
                     LAQPLGAKQFWPVYEEANRLGCCLAVHGGAHDRFGMDHMNMYVPVHALGHPWGLTINC
                     ADILYNGIYERFPRVRIAFLEGGIAWLLLLLERLHASHETHFQHIPEGEFGVREEQDP
                     AKALKKLIKDGRIYLGIETEELTMPFAIKVVGNSPFLYSSDFPHEVTHESCKHDLGEL
                     MESDEITSDDKAAMLWRNAETFYKLPL"
     gene            6656..7738
                     /locus_tag="VMZ02_07450"
     CDS             6656..7738
                     /locus_tag="VMZ02_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUR71804.1"
                     /translation="METCNAAVITEHNKPLTIQKVQIPNLDPGSLLIKITASTLCGTD
                     VHRWHGPLGDKDTLPIITGHEPCGTVQEIAGERTDILGNPVKRGDRVVWSYVACGSCY
                     YCTVACQPCICRGRASWGHNRSDQHPYLLGSCAEYMYVPKECLIIKVPEEVTSASAAA
                     AACAYRTVMHGYDRLGAIKSHETIVIQGSGPLGNFATAVAKDHGAKKVLVIGAPANRL
                     EVTKKMGADAVLNLEEVTDEKQRRQWVKDHTDGRGADVVVQVANNMAVPEGLTLLRDG
                     GRYLNIGAGGKANIAVEKIPQQMLFITIRSGEPRHWLQAIDFLASRRKIFPFEEMISR
                     SYKLDQINEAMEAMANYSVVKAVINF"
     gene            complement(7806..8564)
                     /locus_tag="VMZ02_07455"
     CDS             complement(7806..8564)
                     /locus_tag="VMZ02_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71805.1"
                     /translation="MPGKTHDLTSQTALITGGGSGIGRAMAEALGKAGARVWLASRDA
                     VMIDRAVQELRSVGVVAEGAQLDVTNRAQVENLTEHIVKRDGKIDILLNSAGVMIKHP
                     VLEMPEEIWDRVISVNLTGTFLCTQAVARGMIARGYGRIIHFTSGHVKGNATNSAYAA
                     SKGGVEAFTRSFAAELKQLNVDVTINAVEPGGTDTPMWRRGKSAEYIEQARQSGRIRP
                     ADSMASTIVYLASRASAAVNGQVIERGAKPPPGS"
     gene            complement(8665..9693)
                     /locus_tag="VMZ02_07460"
     CDS             complement(8665..9693)
                     /locus_tag="VMZ02_07460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71806.1"
                     /translation="MVKVFTALLISFITVSKLSAQTPFYQGKTVQVRVGFAAGGAFDV
                     WARVIANHLGRFVPGNPTFVVQNMTGGGSMIAANYIYGVAKPDGLTLGVVSPAMYIEQ
                     ATGKKEVQYDWSKFSFIGSPEKTDRIFYMRADTGIKTLEDMRTVADTPRCGSTGIGSA
                     AYYFPRLLQDSFGLKLTIVPGYPGASDVNLAIEKGEVHCFSGTLQAYFGSEPLRTWSK
                     TGFARVLVQGGNKRDGRMANVPTLWELMDKNKTSEIYRRLARILLIPDEMGRPLFGPP
                     GMPPDRLKILRDGFTKMMMDPELLADARKKGLDAMPMSGEELDALIKEVGLPSADVVQ
                     RLKPLMEN"
     gene            10301..>11449
                     /locus_tag="VMZ02_07465"
     CDS             10301..>11449
                     /locus_tag="VMZ02_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HUR71807.1"
                     /translation="MTIKTQTLLMVTLLLVVAVFATAGVMVWGSRRGLLTESESQGLV
                     IARLLARSAAFGAQVTSDVDKAIGEQMIVEATIAAHLVALGEAAGVGQKEINRRLKEI
                     ADDTVLDEIWITDSKGHAYLRNNTEVDFTFSPDRERQPQAHVFWPLLTGKDKAVVQET
                     RQREIDTQVFKYVGVAGVDKPRIVQVGYRATMLRQLQQRMGLTRLVNQLVSEGSVIAI
                     RVLDKNMITVEYAEKFREQKLPEPSETDLADWRKLVSAGRTDSSLEGSLLNVIAPIGE
                     EGGELKGGAILVTLPTDHVQAAIQNRIRIAMVVSGLVLLLGSMIAILGAKTISRPIAE
                     LTEMTRRIAGGDFTQRIAISAKNEIGVLAGSFNEMTRRLNESIEHLKET"
BASE COUNT         2354 a         3282 c         3099 g         2714 t
ORIGIN      
        1 cttgcttagt cgatgaaatt taatctgctt accagggtgt tgctactcac cctggcggca
       61 cagttcggcc gcggcacaaa tgacccggat cgccgtcggc tacagtggta tcagcgtcga
      121 ccagctggtc atctggattg cgcgcgacgc cggtatattc gctaaaaaca atctcgacgt
      181 tcaggcggtg tacttttccg gtgggacgat ttcggtcacg gcgatgatag ccggcgacac
      241 tccgcttatt caagcctcgg gtccaggcat cgtcagcgat gaaaaaaagt tgccggccaa
      301 gcaatacccg agcgccgagg ggatcaaaac tattcttgac gagcttgctc taaaagagcc
      361 caaggcgagc gccgcccaag ccgagcgatt ttatcgatgc gcgctttgtc gaggaatttg
      421 atcgcagcgg ttacatcgag cggctatacg gtaggaataa ataatctctg tggcaatttg
      481 cgataaaggt gcagcacaag cgcagcgccg cgtaccactc acgacgaaac gctcgcgtta
      541 atccttcagc cgaatcccca ccttggacaa gcggtgtttc catacgccgc ggcgctcgat
      601 gccttgccaa acttcgattt cgtggcgcgc gagctgggtc ttcttttctt ttaattccgc
      661 catgagattt tgaatcaggc tatcgcgggc tttaaggagc tcttcgcgtt cactgagcaa
      721 ggtgtccaaa ccgttggtat cgtcgcctgc ggtgatatcg tcgtcggggc gaatcactgc
      781 cggcagatgc ggtccttgag cgcgcgcttc ttcgagcaat cgttccgtgt ggatcttggc
      841 cacctcggtt tcctggagtt tctcgtgcag cacagcgatc tgctgtctca tagcggtcat
      901 ctcctcgctg ccgctttcga gcaggttttg tttttccgca agctgccgtt ccaagaactc
      961 aagcttgccg ctcaagcgtt cctcgacgtc atcgacagaa gactccttgg cctgcaataa
     1021 cgccgtcttt tcgcgcagct cctcctgcaa gacacgatcg ctgcgctcct tttcgcttac
     1081 cagcgaactg tacttgtccg atatctcgcg tactttgttc atcagcgcgt cgacttcgcc
     1141 ttcgcgcgtc gtcataagat tctgctgttc gctgagtttg gcttcgagcg cttggatctg
     1201 ccctttgaat tgattttgaa gctctgcgag cgcagcttcc ttggcctgca gggcttcttt
     1261 gtgctggcgc aattcgcggt cgagagcgcg cacagactcc tctttggtat tgctcaacag
     1321 gtcgagctgg ccggttaaat ccttaacttt cgaacgcagc gagtcgactt caccacggcg
     1381 agaatccacc aatccctgct tttcagctaa ctgggtttca agcgcacgca aacgatctgc
     1441 gctgcgtttt tccagctctt taagcgccga ctcacgggct cgcaagagat ggtcctttgc
     1501 tttggcgtcg gcttggaact tccgcgccgt ctgatcgcta atcgcatcca tctctgtcag
     1561 ttgccgcttc gcgatttgga gttctagctc gcggttcctg agattttttt ctctgtcctt
     1621 gagttgggct tcgagtgctt gctgtttcgc cagcaattcg gcttgcatct cctgtaccgc
     1681 gctattcttg gtgctaagca gcgacgcctt ttccttgaat gcctcttcga gcatttcctc
     1741 ggcctgacgt ttggccgcgg accattcgct ggccttttgc tgcaccgcaa tcaactccgc
     1801 tcgcaaggca gtgacctccg cgtcgcggct gccgacgagc ttctggcgtt cggccaattg
     1861 ctgctcgaga gcgctgatct tagaaccgaa gttggcctcc aattccttga gagccgaatc
     1921 actggcgctc aggatagctt tttccttgcg cagcttttcc tggagcagac cggcagcgcg
     1981 ctcttttgcc gagccaactt tgctgagttg ctcggccaac gatgcgagtt gggtctggag
     2041 tgcttcgatt tcagtcccgc gagtttgcag caaaccgtca cgctcggtga gttgctgctg
     2101 caaaacgttc agttcggcgg tgtaccgagc ttccaactcc ttgttgcccg actccttcgc
     2161 ttgtcgtatc tccttctcct tgcgcaactc ctgcgtcaag ctcttctcca agttggcgcg
     2221 tgccgtcact tcctcgtcga gttttttggt gaggctttgc acggtcaacg tcaaggattg
     2281 aatctcgcct tctcgaactt gtagaacctg ctccttttcc cctaattgac tttccaactg
     2341 acgcaacgat ttactgtgac gctcttccaa cgcgcgcacc gtcgcagctt tttcatcgct
     2401 gatactctgg cctttactga gcgccgcgtt gagagcagtc tcggcttgtt ggctgaccct
     2461 ttgggcttcg tcgaatttcg cactgagcgc gtgaacttcc gccttgagcg agtggatctc
     2521 accgtcgcgt gcctggacaa gcatatcccg ctcttcgatc tgattctgca gatcgcggat
     2581 gttgttgccg agacgagatc ccagttcctt aacggtgctt tcccgtgcat catgttcctt
     2641 ctccaggtcg gcgcgaacgg ctgccaattg ggattccgcc tgttcctttg ccaaggccga
     2701 taaatgcaac tcggtgttga gagcagcgag ctcactctca tgactcttga gcagacgctc
     2761 acgatcgcca gcttgcgatt cgaggctgcg aatggtttct tgcaaacgtt tctcgatatt
     2821 acgattcgcg gcatcgctag cctcgagaac ttcttttttg ttctgtagct ctttctgtag
     2881 cagttcctcg gcgcgccgcg acgcggcttc caactctttg acctgagcgc tcagttcgtt
     2941 cgtttcactg cgggcaattt ctaactccgc ggcgcgccgc ttgagcagat cctccttggt
     3001 ttgcagttgg ttttcccaga gccgcgcttt gccactcaac tgttgccgca gttccttgat
     3061 ttcgccatcg cgggcgtgga gcgcttcggc gagtcgcatc gtctccgctc gcagggcttg
     3121 atcgccggca tcggcgttga cgaggatcgg tctcggcgtc gagctactag atttgatcgc
     3181 gcgcagctcg tcgtcgcgcg ctttgagcgc ctgctctttt gccgtgagct cgaggatcat
     3241 cgtttccatg cgctcgatgc tgtcctcgag atcgcgcgtg acactgtctt tggcttggcg
     3301 cgcagtctta cctttccaga ccgcatcctg gagcatgtgt tcggcttctt gtttcgccgc
     3361 atccaatgcc tcgacccgct ctttcaggtc catgatttgg ccatctcgga acatctttag
     3421 aacgaagacc atgacgacca atccgaagcc tccgagcacg gcgtaaaacc agacactgct
     3481 ggactctccg gattcagaat taccgaatac aaaagcgatt ggggaggaga taaattggcg
     3541 cagattgcgt ccaaacagcc agcgggtgta attcgcttct atttcgttac cttgcattga
     3601 ataaacaatg atgctcccaa gtatcagggc agcaacgaca acggcgataa ggagtttttc
     3661 ccgggtccgc atagcttcct acattcgttt agtcggcgaa attgatgttt tcgagattgc
     3721 ggtattgtaa cgtctgagaa ggagtttgcc aaaggaaatt gcgctgagaa tcagcccaac
     3781 ttccggtggc aaggaaggaa acgaccggat tttaccaaaa ctactgcggg tgaaggcgcc
     3841 gtattaccgg tgttattgcc gcggaagggc gactcgtcac cgttcggaag cggaatttgt
     3901 cgctgctctg acgaatcgtc aaagcggcgg tgctttaacg atcgtcgagt catcgatgct
     3961 ggcaatggtc ggccggccgc acttggccat ggttatctcg atctcttcac gaagtaaatt
     4021 aagcacgcgt acgactccct gctcgccgcc aacggcgagt ccccaaaaca gcggccggcc
     4081 aatgaatacg gctttggctc ccagagcgat cgccttgacg acatcggcgc cgcggcgaat
     4141 accgccgtcg agcagcactt ccattttccc cttggccgcc gccaccgcct cgctcaacac
     4201 ttcgaccgtc gccaaggtat tatcgaggtg ccgaccgcca tgattggaaa cgatcacgcc
     4261 gtcggcgccg acgtcggcgc agagttcgac gtcttcaccg gtcatgattc ccttggcaac
     4321 gaccggcaag ctggtcgcgg cttttatcca ctccagatcc ttccaagtgg cggctggatc
     4381 ggaaccggcg ccactgccag ccggacgcgg cgcatcggga tgttcgccag cggcgggata
     4441 attggcgcca cgcgtgcgct gataacgatt acggatattg cgttcacgtt tgggcggcca
     4501 ctgtgaatca agcgtcacga caatcgcctt gtaaccgttc gccttggcac ggtcgagcca
     4561 attttgcgtc atcgatttat ccctgaaggg atagagctgc acccataacg tcccattcgc
     4621 ggctttggcg agatcttcaa aagaagtatt ggcgttggcg ctcaccgcta gcacggtacc
     4681 gcaggaggct gccgctcggt aggtggcaat ctcgccttca gggtgggcac gtttgtgacc
     4741 gccacacggc gcaatcaata ccggcaactc aactcggctt cccaaaaccg tcgtcgacaa
     4801 atcgagctta ctgacatcgc gcaaaacccg cggccgaaac gcccacgatg cgtaggcgcg
     4861 gcggttacga tccacactga tctcatcggt cgcgcctcct gcgatgtagt cgtattcctc
     4921 tttcgccagc aacgatttgg cggcatcttc gaactcaaac agattgacgg gttgcgcagg
     4981 ggttgccata aaaactccaa atgatttgag cgcgagagat gattagctta ttgcttggcc
     5041 ttgagttgct gatcattgtt cttgagaaat tcgcggatct catcggcgtg ctcgagaagt
     5101 ctattccact gttctttata aagcgtcacg ggaaatcggc ccaagccgta aattgatagc
     5161 ccgccctttt cgctgacctt caagcgtagt tctccgggct tgcgatcgac ctgtttcttg
     5221 agtgcctgat tttcggcttc gaggcgtgcc agctttgctt cgatggactc ttcagccata
     5281 gatcctccgt cattcgttgg cagatattgt tatcggaaac ttctatcgca aagttcgcga
     5341 caacgcgagt ccaatgcgag cgcaaccggc ttgaaccgca tcctaaagtc ggattataag
     5401 atagacgatg gatttgtaat caatcagcga aaatattcag ggggaattta tccatgagcg
     5461 caaaccttgg cacgcgaata atcgacggcg acggtcatat attggaagac aacgcgggca
     5521 tcatcgccca tatggaaggt gcataccggg aaatcgccgg gcgcaaaggc acctgctttc
     5581 cgccgctgga tcaccttcat tccgggcgcg ctgttgaaac cccgccgcaa cgtgatggac
     5641 gggcgccggt tggcccggca ggctggctcg attttcttga cgatatcggc attgaatgga
     5701 ccgtcatgta tcccactctg gcgttgtcct acggcaagat cgtcagccgc gactacgctg
     5761 tcgcggtatg taaagcctac aacgattgga tgtcggagac gtatttgaaa tacaatccgc
     5821 gcttcaaagg tatggcgatc attccaatgc aggacccgga agaggccgcc aaggagctgc
     5881 gtcgtgtggt taccgaattg ggcatgctcg gcggcatgat gccatccaac ggtctcgctc
     5941 agccactcgg cgccaaacag ttttggccag tctatgaaga agccaaccgc ctgggctgct
     6001 gcctcgccgt tcatggcggc gcccatgatc gtttcggcat ggatcacatg aacatgtacg
     6061 tgcccgtgca tgcgctcggc catccttggg gcctgacgat caattgcgcc gacattctct
     6121 acaatggcat ctacgagcgc ttcccacgcg tgcgcatcgc cttcctcgaa ggcggcatcg
     6181 cctggctgct gctgctgctc gaacggctcc acgcttcgca tgaaacccac tttcaacata
     6241 ttcccgaagg tgagttcggt gttcgcgagg aacaggatcc cgccaaagcg ctaaagaaac
     6301 tcatcaagga cggccgcatc tacctcggca tcgaaaccga agagctgacc atgcccttcg
     6361 caatcaaagt cgtcggtaat tcgccgttcc tctactcgtc agactttccc catgaagtca
     6421 cccacgagag ctgcaagcat gacttgggcg agttgatgga aagtgacgag atcacctcgg
     6481 acgacaaagc cgccatgctt tggcgcaacg cggaaacgtt ctacaaattg ccgctgtaga
     6541 tcgcgctatc cgattagaac cgctggaacc tttggaaccg ccggaacggt gctaatcgtt
     6601 ccagcgggct ccaaatgttt tcaaagctaa ccaccgcacg aggaaagaaa gatttatgga
     6661 aacttgcaat gccgccgtca ttaccgaaca taacaagccc ctgacgattc aaaaggtgca
     6721 gatccccaac ctcgatccgg gctcgttgct gatcaagatc accgcctcga cgttgtgtgg
     6781 caccgatgtg catcgttggc acggcccatt gggcgacaag gacaccctgc cgattattac
     6841 cggccacgag ccctgcggca ccgtccaaga aatcgccggc gaacgcaccg acattttggg
     6901 caacccggtg aaacgcggcg atcgcgtggt gtggagctat gtcgcctgcg gttcctgtta
     6961 ttattgcacc gtcgcctgtc agccttgcat ttgccgcggc cgcgcttcgt ggggccataa
     7021 ccgcagcgat caacatccct atctgctcgg cagctgcgcc gagtacatgt atgtgccaaa
     7081 agaatgcttg attatcaaag tccccgaaga agtgacctcg gcgtcggcag ctgccgcagc
     7141 ctgtgcgtat cgcacggtca tgcacggcta tgaccggctt ggcgcgatca aaagtcatga
     7201 aacaatcgtg attcaaggca gtgggccgct cggcaacttc gccaccgcgg tcgccaaaga
     7261 tcacggcgct aagaaagtct tggtcattgg cgctcccgcc aatcgtttgg aagtcaccaa
     7321 gaaaatgggc gccgacgcgg ttttgaatct cgaagaagtg accgatgaaa agcagcggcg
     7381 tcaatgggtc aaggaccaca ccgacggacg cggcgccgat gtcgttgttc aggtcgccaa
     7441 caacatggcg gtgccggaag ggttgacgct gctgcgcgac ggcggccgtt acttgaacat
     7501 aggcgccggc ggtaaagcga atatcgccgt ggagaaaatc ccgcaacaga tgttgttcat
     7561 cacaattcgc tcgggcgaac cgcgccattg gctgcaagcc attgattttc tcgcctcacg
     7621 gcgaaagatc tttccgttcg aggagatgat cagtaggagt tacaagcttg atcaaatcaa
     7681 tgaagccatg gaagcgatgg cgaactactc ggtggtcaaa gccgtgatca atttctgaac
     7741 aggcgggagc gaaaggcccg atcgtaattt acattcgccg ctaatcgatt ttattgtcgc
     7801 ttaaactatg aaccgggcgg cggcttggcc ccccgctcga tcacttgccc gttcaccgcc
     7861 gcgctcgcgc gactcgccaa gtatacgatc gtcgaagcca tggaatccgc cggacgtatc
     7921 cggccggatt gccgcgcttg ctcaatgtac tccgcgcttt tgccgcgacg ccacatcggc
     7981 gtatcggtgc cgcccggctc gacggcgttg atcgtcacat cgacgttgag ttgtttcaat
     8041 tccgcggcga agctgcgtgt gaacgcttcg acaccgcctt tggacgctgc ataagccgag
     8101 ttggtcgcgt tgcctttcac atggccggaa gtaaagtgaa tgatccggcc gtagccgcgc
     8161 gcgatcatgc cgcgtgccac cgcctgcgtg cacaaaaaag tccccgtcaa gttgacgctg
     8221 attactcggt cccaaatctc ctccggcatc tccagcaccg gatgttttat catcacgccg
     8281 gcactgttga gcaggatgtc gatcttgccg tcccgtttga cgatgtgctc ggtcagattt
     8341 tctacctgag cacgatttgt gacatcgagt tgcgcgccct ccgccacgac gccgacagaa
     8401 cgcaattcct gcaccgcgcg gtcgatcatc acagcgtccc gtgacgctaa ccaaaccctc
     8461 gcaccggctt tgccgagggc ttcggccatc gcccggccga ttccgctacc gcctccggta
     8521 atcagcgctg tttggctagt gagatcgtgg gtctttcctg gcatgtttaa tatttgtctc
     8581 agctgcgccg agcgcgtttg gcctggtttc tcgtttgaat aaatcgtgat tgcttgtccg
     8641 ttccttgcgg cgaacttcct gcggctagtt ttccataagc ggtttcaacc gttgcacgac
     8701 atcggccgac ggcaagccga cctccttgat cagtgcgtcg agttcctctc cgctcatcgg
     8761 catagcgtcg agtcccttct ttctcgcgtc ggcaagtagc tccgggtcca tcatcatttt
     8821 ggtgaaacca tcccgcaata ttttcaaacg atcaggcggc atgcccggcg gtccaaagag
     8881 cggccggccc atctcgtcgg gaatgaggag aattcgcgct aaacggcggt aaatctcact
     8941 ggtcttgttt ttatccatca attcccacag cgtcggcaca tttgccatgc ggccatcccg
     9001 cttgttaccg ccctgaacca gcacacgggc aaaaccggtc ttcgaccaag tgcgcagcgg
     9061 ctcgcttcca aagtaggctt gcagcgtacc actgaagcag tgcacctcgc ccttctcaat
     9121 ggcgagattg acatccgatg ctccaggata acctggcacg atcgtgagct tgagtccgaa
     9181 gctatcttgc aacagccgcg ggaaatagta ggccgcgctg ccgatgccgg tggagccgca
     9241 gcgcggtgta tccgccaccg tgcgcatatc ttcaagagtt ttgatgccgg tgtcggcgcg
     9301 catgtagaag attcgatcgg tcttctcagg cgagccgatg aaactaaact tggaccaatc
     9361 gtactgaact tcctttttcc cggtcgcttg ctcgatatac atcgccgggc tgacgacgcc
     9421 gagcgtcaat ccatccggtt tggccacgcc gtaaatgtaa ttcgccgcaa tcatcgaccc
     9481 gccaccagtc atattctgaa caacgaatgt tgggttcccc ggcacgaacc gtccgagatg
     9541 gttggcgatc acccgcgccc agacatcgaa cgctccaccg gcggcgaacc cgacacggac
     9601 ctgtacggtc ttgccctggt agaagggggt ctgggccgac aattttgaca ccgtaatgaa
     9661 actgattaac aaagctgtga aaactttcac catttaactt ctccgtgttc tatgcgaatt
     9721 ccgctggggc taagatcaat caaatatagt gaacgtgcaa ctggcaattc taaaatttaa
     9781 cacttcccga tctttgccta gaaccggcgt ccagctcgat cccaagtagc ttgaggtcgt
     9841 catcgaaatt atcggtgcat caaaaccggc gctctctgca ggaatattac tagtacaccg
     9901 gtccgtgtat cgaaatgaac gctcatgaga ttgattgaca actccttgct caaaagcgta
     9961 aaccttgcgc agtcgaggat ttttgaatcg gcaatattga tccaatcatt cggctagccc
    10021 ctacgaactc cgagggccaa cttcaacatg aaatttttct attagtgagc cttaggttca
    10081 tgccttctgt acgtgacgaa cctggttgaa cccattgtcc gtatgctgca ctggtcgggg
    10141 cacgctgcgg tgcggttcac agattctcgt gcgttagcac gtctcgcggt ccggccattg
    10201 ccggattcaa atgcatttcg atgatggaca atatggagat catcttattt tttcgatcgg
    10261 caatgtgctg cggacggact gagcggcgaa ggagaagttt gtgacgatca agacacaaac
    10321 gcttttgatg gtgactctgt tgctcgtcgt cgcggtcttt gccacggcag gagtcatggt
    10381 ttggggctcg cgccggggat tgctgacgga gagcgaaagt cagggtttgg ttatcgcgcg
    10441 gcttttggcc cgcagcgccg ccttcggcgc gcaagtgacc agcgatgtcg ataaagcgat
    10501 tggcgagcag atgatcgttg aagccaccat tgcagcgcat ttggtagctt tgggggaggc
    10561 ggcgggagtc ggccagaagg agatcaaccg gcgcttgaaa gaaatcgccg acgacacggt
    10621 gctggacgaa atctggatta ccgacagcaa aggtcatgct tacttaagga ataacacgga
    10681 agttgacttc acgtttagtc cggatcggga gcggcaacct caagcgcacg tcttctggcc
    10741 tttgctcacg ggtaaggaca aagctgtcgt ccaggaaacg cgccaacgcg aaatcgacac
    10801 gcaggttttc aagtacgtgg gggtcgcggg ggtggataag ccgcgcatcg tccaggttgg
    10861 ttatcgcgct accatgctgc gacaactgca gcagcgcatg gggttgactc ggctggtcaa
    10921 tcagttggtg tctgagggaa gcgtcattgc catccgcgtc ctcgacaaga acatgatcac
    10981 cgtagagtat gccgagaagt tccgcgagca gaagcttccg gagcctagcg agaccgactt
    11041 agcggattgg cgcaagctgg tgagcgcagg gagaaccgac agctctctgg aaggatcttt
    11101 gctcaacgtc atcgctccga tcggcgaaga aggaggagaa ctgaaaggtg gagcgattct
    11161 cgtcacgctc ccgacggatc acgtgcaggc tgccattcag aacaggattc gaatagccat
    11221 ggtcgtgagc gggttggtat tgttgctcgg gtcgatgatc gccattttgg gcgctaagac
    11281 gatttctcgg ccgattgccg agttgacgga gatgacgcga cggatcgccg gcggtgactt
    11341 cactcaaagg atcgccattt ccgcaaagaa cgaaatcggc gttctagccg gctcgttcaa
    11401 cgagatgacg cggcgcttga acgaatctat cgagcatctc aaggaaacc
//
LOCUS       DATLCH010000254        12468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2174591, whole genome shotgun sequence.
ACCESSION   DATLCH010000254 DATLCH010000000
VERSION     DATLCH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174591"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VMZ02_07470"
     CDS             complement(<1..617)
                     /locus_tag="VMZ02_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HUR71808.1"
                     /translation="MRRKKVCFVGYGSTEYSRKSEESLLGQYAIAIRAALNQTGLKKK
                     DINGLSVTTQASPDTAPHVAEQLGFELDWVLNADYGGASGVGAVSRAADAIECGHLDI
                     ALLLAGNSFDRSVAHQRQLEYQRGNFVDVYGYGGPNSLMALIQRLHMENFGTTLEQVG
                     KIAVAQRFNAINNPQALFREPMSMQDYLNSRMISDPVRLFDCVMP"
     gene            complement(722..1843)
                     /locus_tag="VMZ02_07475"
     CDS             complement(722..1843)
                     /locus_tag="VMZ02_07475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR71809.1"
                     /translation="MDLKLSEEEKLIKDTAAQFVDKELIAREGAFLKQAQPFLPPGAP
                     ERRELDAGIAADLANKARAVGLWALELPEAMGGSAMGHVAQVLIHREFGRSVLPFAPA
                     VIPAFMFETKYGQALAAGESSLGLAFDEAHQIGSLSHLHTLYRQEPEGAILQDSRMNI
                     FTPEADLFLFPAREQGTDRLGLFVIERDAPGLNILDAADLTTDSTIALLSLRHCRIAN
                     EQLLGYEYEAGAVIASEQLRIAARSLGIGMRCLTASIEHAKNRVTFGRRLAERQAIQW
                     MLADLSIEIRTTTWITLEAAWRADNDLPYFDEAALAKKRAARMAFQAADSAIQIHGGY
                     GVCKEFPFESFYREARLMRLLYGREEEMNRAAGEKFLRQ"
     gene            complement(1949..3145)
                     /locus_tag="VMZ02_07480"
     CDS             complement(1949..3145)
                     /locus_tag="VMZ02_07480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR71810.1"
                     /translation="MNFHLSAEEESLRESVAEFVRTELLPLEPEFAYAPDIFEGSRWK
                     SRVRSSADPEIKRYLEIMEGIEKRAEANGLWHLDVPKAYGGMEVSNVAMIAVTEELEK
                     SSIPFEFGNHVSNILFSCTGEQIDKYLWPCIRGEKTSAFGLSEPASGADPSMMQTTAT
                     PDGEYFEINGTKMFPTFADVADFVQLFARLPGTKGREGVTCFLIDTGTPGYRVARSIA
                     TIAGTEPCELVFEDCRVHKSQVLGEVGNGWALNQAWLGARRFQVGIRAHGMAQRVLTA
                     VAQVLRQNDKEAEKHAGSFGHLLGELEALKALTYSASWRADEKMDVRVDAAKVKLFGT
                     ELAHRVIDFAIEAVGPSALRKEHVIARAFRHARARRIIEGPSEVQRHIIQRELFRQGS
                     SLLEID"
     gene            3293..4315
                     /locus_tag="VMZ02_07485"
     CDS             3293..4315
                     /locus_tag="VMZ02_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71811.1"
                     /translation="MSAVNLANVNIIDADGHVLDRDANLRKFIPEPYCRRQGSLLPND
                     VWDASMYGKLITKSPDVPTRLRDMDTEHIDVSVLFPTGGFGVTQLPEKDYAAAFCRGY
                     NDWIASVCAESPRLKGVGLVPFQDVDAAAKETQRAIGELGLAGITVGTFGMKEHLGQP
                     MYWPIYEQLEKFNVPLLVHNSRQGPAGEIRFDTFLFRHTIGRPFETLLDCAALVYGGV
                     PEKFPKLRIAFLECGCGWVPYWMDRMDEEWEKRQAEAPLLKKKPSEYITAGNWFFAAE
                     PEESTVPFCLDKVGEDVILFASDYPHWDGGFPYMTATVKGRNDITEAQKNKIMRDNAA
                     RLYGWN"
     gene            4331..4825
                     /locus_tag="VMZ02_07490"
     CDS             4331..4825
                     /locus_tag="VMZ02_07490"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HUR71812.1"
                     /translation="MKFRASALFSLVVLIFFCVFVYEAKEWRMQARLYPFVIGIPMLI
                     FAIVQFVLDLKGVKAKESATDGAPMDFQFQQTDIPDDVVRKRTITMFAWMFGFFAMIW
                     LVGYVIAIPLMVFCYLKFQSNESWVLSTTLTVIAFVFFYFLFVRLLNLPFPDGALQTM
                     LGLV"
     gene            5007..6101
                     /locus_tag="VMZ02_07495"
     CDS             5007..6101
                     /locus_tag="VMZ02_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71813.1"
                     /translation="MKNKVVSPFKRSKVQGFNRILFGLLFLSLELLNPLNLEPIFAQA
                     NFYQGKTVRVVIGFTPGGFYDRWARLLFRYIPKYIPGNPNFVVQNMPGASSVIAANYV
                     YNLAKPDGLTMLVPINSLYLDQIVGRTEVKFDMRKFEFIGTQEKTPTMLYFRSDSPFK
                     TLNDIIQAKEPPKCGSTGTASTGYLLAKIMDEAFKAKMGTVTGYQGGSEIDIAVERGE
                     IVCRGMDIPPHFGREPFDSWHKKGFDRHILQSGPKHDPRMGDVPTLFELMDQFKTPDV
                     MRGAARIIMASGEFGRPMLAGPGNPPERIKMIRDAYAKAMRDPGLIEEAKKGQMDMEY
                     TSGEDLQTLMKELMNQPREVIERVKKILAD"
     gene            6109..7197
                     /locus_tag="VMZ02_07500"
     CDS             6109..7197
                     /locus_tag="VMZ02_07500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71814.1"
                     /translation="MKIKNQFKSSKVQGFNRILLGFLFLTLEVLNPLNLEQASAQSEP
                     FYKGKTVRIVVGLTPGGFYDRWARMLARFMPLYLPGNPNFIVQNMAGASSMIAMNHVY
                     GVAKADGLTLGMPNYGVYLDQLAGRQEVRYDITKMHYIGSPEKSDIVLYARADAPFKT
                     VQDMRKLPEPAKCGASGTASADYLIAKVLDDTLGVKINSVLGYAGGSEIDIAVEKGEV
                     LCRGMTIAPHFGREPFDSWHKKNFDRHIVQTAEKRDSRIGDVPTLYEIFEKENTADES
                     RRVADVILRGGDFGRPMLVGPGTPLDRVNMLREAYVKSMNNPELIAEAKKTRMDMEPV
                     TGEQLQALAKRVMNQPPAVLKRVKKLLE"
     gene            7249..8280
                     /locus_tag="VMZ02_07505"
     CDS             7249..8280
                     /locus_tag="VMZ02_07505"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71815.1"
                     /translation="MKAAIILLFLLGAGPAHLAAQTPYYQGKQVKVIVGFTTGGFYDR
                     WARILSRYMPKHIAGNPSFVVQNMPGAGGLTATNYVYSVAKPDGTTIGFPSNGLYLDQ
                     LVGRKEVQYDIRKFAWIGSPVKEPAIFYMRADTPFKTIQDVKNSKEPPKCGSTGTAST
                     DFILARLLEDTLPPLKITTVQGYPGGAEIDLAVEKGEVACRGMTASPFFGREPFLSWQ
                     KKNFVRVLLFTGDKRDPRMPDTPTLDEIFAKEKVPEAGRRVAEVILAAESFGRPIIGT
                     PGTPAERVSELRRAFEQAMKDPELLAEAQKQKMDVDPVSGENLEKLATRILDQPPEVL
                     ARVKKILGN"
     gene            8344..9372
                     /locus_tag="VMZ02_07510"
     CDS             8344..9372
                     /locus_tag="VMZ02_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71816.1"
                     /translation="MLKLLAFLSVFIVGQVAHAQTPFYQGKTITIVVGTKAGDAYDLY
                     PRMLAEFMPKYIPGNPNIIIQNVPGAASMIAANQVYNVSKPDGLTIGAIYPALYFEQL
                     TKKPEAKFDWTKFGWVGNPVTSNHLLYMRSDAPFKSMDDIKNAKEAPKCGSNGITSTG
                     YYLPKLMEETLGTKFDIVLGYQSGQDVDLAVERGEVVCRAFTITAYFAREPFISWRKR
                     GFVNALMQSGLKREARLKDAPTIYELMDKYKTNAAGRSLAKVVLSAGEFGRPLVVGPG
                     VPADRLKILRDAFDKSVTDPALIATAEKRRLEMDPASGAELEALAKEVMAATPDVVQR
                     MEKLLGGK"
     gene            9464..10489
                     /locus_tag="VMZ02_07515"
     CDS             9464..10489
                     /locus_tag="VMZ02_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71817.1"
                     /translation="MFSTLVALAALAWSSTLFAQTSFYQGKTITIAAGTKAGDVYDLY
                     PRLLAEYMPKHIPGGPNIIVQNIPGAASLIAANQVYNTGKPDGLSLAAIYPALYFDQL
                     IKRPEVKYDWAKWQWLGSPVKSNQLLYMRADTPYKSMDDIRKAATAPKCGSTGIASAG
                     YYIPKLLEEVLGSKFDVVSGYQSGQDIDLAVERGELHCRAFTITAYYAREPFISWRKR
                     NFTNVLVQTGSQRDARIKDSPTIYELMDKYKTSGTGRTLAKVILASGDLGRPIVAPPG
                     VPADRIKILRDAFDKTVADPAFLSEAEKRRLEIDPTKWDEMESLAKEVMTTPADVVAR
                     MRKLLGE"
     gene            10582..11610
                     /locus_tag="VMZ02_07520"
     CDS             10582..11610
                     /locus_tag="VMZ02_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71818.1"
                     /translation="MVPSIVILLSVLLWPSGGYAQTPFYKDKTIRLVAGTPAGSVYDL
                     YARMVAQFIGKYIPGNPNVIVQNMPGVASMVAANYIYTVAKPDGLTIGSIQPALYFDQ
                     LVGRKEVQFNWQKFTWIGNTTVSHLLLYMRGDTPYKTIDDIRKAAVPPKCGAEGTASS
                     AYYLPRLLEETIGAKFNVVLGYNSGTDVDLAVERGEVQCRAFTVAAFFAREPFITWRK
                     KGFVRVIVQTGKQRDSKLPDVPALQELMEQNKSAEASRRLAAVLLAANEFGRPIIASP
                     GIPADRVKILREAFLKTITDPDLLEDAKKKRLDLDPVSGEELESLAKEIMVQPPDVIE
                     RMKKLLGT"
     gene            11656..>12468
                     /locus_tag="VMZ02_07525"
     CDS             11656..>12468
                     /locus_tag="VMZ02_07525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR71819.1"
                     /translation="MIRAAFVRSVLFVLLALLFSTRLHAQTPFYQGKTIQIIVGAKAG
                     DTYDLYPRLVAEFMSKHIPGNPNIIIQNVAGAAGLIGTNQVYNLAKPDGLTLGAIYPA
                     LYFEQLAKRPEAKFDWTKFIWIGSTVSSNSYMYMRADTPFKTIEDVRTAATPPKCGAT
                     GVTSSAYYMPKLLEEAIGTKFEIVSGYVAGSDIDLAVERGEVQCRAFTINAFFSREPF
                     ITWRKKNFVRVLYQTGSKRDARLKDVPLLTELMDKYKTPENVRRLAKVVLAAD"
BASE COUNT         2876 a         3634 c         3316 g         2642 t
ORIGIN      
        1 cagggcatga cgcagtcgaa tagacgcacc gggtcggaaa tcatccgcga atttagatag
       61 tcctgcatcg acatcggctc gcgaaacagt gcctgcggat tgttgattgc gttgaagcgc
      121 tgcgccacgg cgatcttgcc gacctgctcc aaggtcgtgc cgaagttctc catgtgcagc
      181 cgttgaatca acgccatcag cgagttgggc ccgccgtagc cgtagacatc gacgaagttg
      241 ccgcgctgat attcgagctg gcgctggtgc gcaacgctgc ggtcaaagga gttacccgcc
      301 aacagcaaag cgatgtccag atggccgcat tcaatggcat ccgcggcccg gctgaccgcg
      361 ccgacgccgc tggcgccgcc gtaatcggcg ttgagcaccc aatcgagctc aaaccccaac
      421 tgctcggcga catggggtgc cgtgtcagga ctcgcttggg tggtaacgga caagccgttg
      481 atgtctttct tttttagtcc ggtctgattg agcgccgcgc gaatcgcgat cgcatactgc
      541 cccagtagcg actcctccga tttgcgcgaa tactcagtcg atccataacc gacaaaacaa
      601 acttttttac gtctcattct ttgctcgctc ctttcagtcg cttcgaggca ataggcagta
      661 ggcaacaggc attagtaaaa actctgatcc ggttttcata ctgttgccta ttgcctgttg
      721 cctactgcct taaaaacttc tcccccgccg cccggttcat ctcttcctcg cggccgtaca
      781 gcagccgcat caaacgcgcc tcgcgataaa aactttcgaa gggaaactct ttacagacgc
      841 cgtaaccgcc atgaatctga attgcgctat cggcggcttg gaaagccata cgcgcggcgc
      901 gttttttggc caatgccgcc tcgtcaaaat acggcaggtc gttgtcggcg cgccaggcgg
      961 cttccaacgt gatccaagtc gtcgtgcgaa tctcaatgga gagatctgcc agcatccatt
     1021 gtatcgcctg gcgctccgcc agccggcgcc cgaaggtgac gcgattcttg gcatgctcga
     1081 tggacgcggt caggcagcgc atgccaatgc ccaacgaacg ggcggcgatg cgcagttgtt
     1141 cactggcaat aaccgccccg gcctcatact cgtagccgag cagttgctca ttggcgatgc
     1201 ggcagtgccg caatgatagt agagcgatcg ttgagtcggt ggtgagatcg gccgcgtcga
     1261 gaatattgag gcccggggca tctcgttcaa taacaaagag acccaaacga tcggtgccct
     1321 gctcccgcgc cggaaacagg aacaagtcag cctcgggcgt gaagatattc atgcgcgagt
     1381 cttgcaggat cgcgccttcc ggctcctggc ggtacagcgt gtgcaggtga gacaagctgc
     1441 cgatttgatg cgcttcatca aaggctagac ccaacgacga ttcacccgcg gcgagggctt
     1501 gaccatattt agtttcaaac atgaaagccg gtatcaccgc aggtgcaaac ggcagcaccg
     1561 agcggccaaa ctcgcgatga atgagcacct gggcaacatg gcccattgcc gacccgccca
     1621 ttgcttccgg caattccaat gcccacaaac cgactgcgcg cgccttgttg gcaagatcgg
     1681 ccgcgatacc cgcgtcgagc tcacgccgct ccggcgcccc tggcggcaag aacggctgcg
     1741 cctgtttaag gaacgcgcct tcgcgggcaa tcagttcctt atcgacaaac tgcgccgcgg
     1801 tgtctttaat cagcttttct tcttcggaca actttaagtc catcggtcgg gcttttttct
     1861 ttccttgaac cttgaaccta tgaacccttg aacgaaaagt ttggttcaac gttcaaaagt
     1921 tcacgtgttc aatcgaacca acacttggct aatctatttc taataagcta cttccctggc
     1981 gaaacagctc gcgttgaatg atgtgacgct gcacttccga cggcccttca atgatgcgcc
     2041 ttgcgcgcgc atgacgaaaa gcgcgcgcga tcacatgctc tttgcgcagc gccgagggac
     2101 cgacggcttc aatcgcgaaa tcgatgactc gatgcgccag ctcggtgcca aacaatttaa
     2161 ctttagcggc atcgactcgc acgtccattt tctcgtcggc acgccacgac gccgagtaag
     2221 tcagtgcttt gagcgcttcc aactcgccga gcaaatgtcc gaagctgccg gcgtgcttct
     2281 cggcttcttt gtcgttttgc cgcagcacct gagcaaccgc cgtgagcaca cgctgcgcca
     2341 tgccgtgggc gcgaatcccg acttggaaac gacgcgcgcc aagccacgct tggttgagcg
     2401 cccaaccatt gcccacctca cccaagacct gcgatttgtg cactcgacag tcttcaaaca
     2461 ccaactcgca cggttctgtg ccggcgatcg tggcaatgct gcgcgccact cggtagcccg
     2521 gtgtgccggt gtcaatgaga aaacaggtca cgccttcacg tcccttggtt cccggtaagc
     2581 gggcaaacaa ctggacgaag tcggcgacat cggcaaaggt cggaaacatc ttggtgccat
     2641 tgatctcgaa atactcgcca tccggggtcg ccgtcgtctg catcatcgaa gggtccgcgc
     2701 ccgacgccgg ctcactgagt ccgaaggctg aagttttctc cccacggata cacggccaga
     2761 gatatttatc gatctgctcg cccgtgcagc taaacaaaat gttcgacaca tgattgccaa
     2821 attcgaacgg gatcgaactt ttctccaact cctcggtgac cgcaatcatg gcgacgttac
     2881 tgacttccat accgccatag gctttgggca catcgaggtg ccacaaacca ttggcctcgg
     2941 cgcgcttctc gatcccttcc atgatctcca ggtagcgttt gatctcggga tcggcgctcg
     3001 acctgacccg gctcttccag cggctacctt cgaagatgtc cggcgcatag gcgaactccg
     3061 gctcgagcgg cagcagttcg gtgcggacaa actccgccac cgattcgcgc aggctctctt
     3121 cttcagcaga caagtgaaaa ttcatcgatc aacgatctcc ctccgcccct ttatcacagc
     3181 ttgctcggac aaaaaaacag ttgccaacca ttgaccacgg attcacgcgt gtgctaagcc
     3241 gatcagctat cgaaactttg cgcgtgaacg aaacaaatat gatggaggtc cgatgtcggc
     3301 tgtaaatctg gccaacgtca atattatcga tgccgatggt catgttctgg atcgcgatgc
     3361 gaatctgcgc aagtttatcc ccgagcccta ctgtcggcgc cagggttctt tgctgcccaa
     3421 cgacgtttgg gacgccagca tgtacggcaa gctgatcacc aaatcccccg atgtgccgac
     3481 ccgtttgcgc gatatggata ccgaacatat cgacgtctcc gtgttgtttc cgaccggcgg
     3541 cttcggggtg acgcaactgc cggaaaaaga ttatgccgcc gcgttttgtc gcggctacaa
     3601 cgattggatc gccagtgtct gcgccgagag tccaagattg aaaggcgtcg gcctcgtgcc
     3661 gtttcaagat gtcgacgccg cggccaaaga aacccaacgg gcgatcggcg aactcggact
     3721 ggccggcatc accgtgggaa ccttcggcat gaaagaacat ctcggtcagc caatgtattg
     3781 gccgatctac gaacaacttg aaaaattcaa tgtgccgctg ttggtgcaca actcacgcca
     3841 aggtccggcc ggcgaaatcc gcttcgacac gttcttgttt cgtcatacta ttggacggcc
     3901 ctttgaaact ttgctagatt gcgctgcgct ggtttacggc ggcgttccgg aaaaatttcc
     3961 caaactccgc atcgcctttc tcgaatgcgg ctgcggttgg gtgccttact ggatggaccg
     4021 catggatgag gaatgggaaa agcgtcaagc cgaagcgccg ctgctaaaga aaaaaccgag
     4081 cgaatacatt accgcgggca attggttttt cgccgccgaa ccggaagaga gtacggtgcc
     4141 gttttgtttg gacaaggtcg gtgaagacgt cattttgttc gcttcggact atccccactg
     4201 ggatggcggt ttcccctata tgacggcgac cgtcaaaggt cgcaacgaca tcaccgaagc
     4261 gcaaaaaaac aaaatcatgc gtgacaacgc ggcgcggctt tacggctgga attaactaaa
     4321 ggagtctcat ttgaaatttc gagcgtcggc attgtttagc ctggtcgtgc tgattttttt
     4381 ctgcgttttc gtctatgaag ccaaagaatg gcgcatgcag gcgcggctct atcctttcgt
     4441 catcggcatt cccatgctga tctttgcgat tgttcagttc gtcctggatc tcaaaggcgt
     4501 caaggccaag gagtcggcga cggacggcgc gccgatggac ttccaatttc aacaaacaga
     4561 catccccgac gatgtggtgc gcaagcgcac cattacgatg ttcgcctgga tgtttggttt
     4621 ttttgcaatg atctggctcg tcggttacgt cattgcgatt ccgctgatgg tgttttgtta
     4681 tctcaagttc caatcgaacg aaagctgggt gctctccacc acgctgaccg taatcgcctt
     4741 cgtgtttttt tatttcctgt ttgtccggtt gctcaatttg ccgtttcccg atggcgccct
     4801 gcagaccatg ctcggcctcg tctaattctt gttttcgaat tcctatccgg tagaaccttc
     4861 ctcggcgcgg gatataattt ggcgccggct tgtctcacat cccgaagcta atatattgac
     4921 ggaacctcgc ccgcaggagg gtgaattcca tgaaaatctc cgaccgctca ccttgccgcc
     4981 attttcttaa gcgattgagg taatctatga aaaataaagt cgtaagcccg ttcaagcgtt
     5041 caaaggttca agggttcaat cggatcttat tcggcctgct ctttctaagc cttgaactct
     5101 tgaacccctt gaaccttgaa ccaatattcg cgcaagcgaa tttctatcaa ggcaagacgg
     5161 tgcgcgtggt catcggcttt actcctggcg gattctacga ccggtgggcc agactcctgt
     5221 tccgctacat accaaaatac atacccggca atcccaactt cgtcgtgcaa aatatgcccg
     5281 gcgccagctc cgtgattgcg gctaactatg tctacaatct ggccaaaccc gacggtctta
     5341 ccatgctcgt gccgatcaat agtctttacc tcgatcaaat cgtcggccgc acggaagtta
     5401 agttcgatat gcgcaaattc gaattcatcg ggacgcaaga aaagaccccg acgatgctct
     5461 attttcgctc cgacagtccg ttcaagacat tgaacgacat tatccaagcg aaagaaccgc
     5521 ccaagtgcgg ctcgaccggc acggccagca ccggttactt gctggcgaag atcatggatg
     5581 aagccttcaa agcgaagatg ggcaccgtaa ccggctatca aggcggcagc gaaatcgaca
     5641 tcgccgtcga acgcggcgag atcgtctgcc gcggcatgga catcccgccg cactttggcc
     5701 gcgagccctt cgatagctgg cacaagaagg gcttcgaccg gcatattcta cagagcggcc
     5761 ccaagcacga tccgcgcatg ggcgacgtac cgacgctttt cgagctgatg gatcagttta
     5821 aaactcccga cgtaatgcgc ggcgccgccc ggattatcat ggccagcggc gagtttggcc
     5881 gaccgatgct cgccggcccc ggtaatccgc ccgagcgaat caaaatgata cgcgacgcct
     5941 acgccaaagc gatgcgtgat cccggactga tcgaggaagc caagaagggg caaatggaca
     6001 tggagtacac ctcgggcgaa gacctgcaaa cattgatgaa ggaactgatg aatcagcccc
     6061 gtgaggtcat cgaacgggtc aagaaaattc tcgcagactg aggggcttat gaaaatcaaa
     6121 aaccagttca agagttcaaa ggttcaaggg ttcaatcgga tcctcctggg ctttcttttt
     6181 ctgacgcttg aagtcttgaa ccccttgaac cttgaacagg cgtcagcgca aagcgaaccg
     6241 ttttacaaag gcaaaaccgt ccgtatcgtc gtcggcctga cgcccggagg tttttacgat
     6301 cgttgggcgc gcatgctcgc gcgcttcatg ccgctttatc ttccgggaaa tcctaacttc
     6361 atcgtgcaaa acatggctgg cgcgagttcg atgatcgcga tgaaccacgt ctacggcgtc
     6421 gccaaagccg acggcctgac tctgggcatg cccaactacg gcgtctatct cgaccagctc
     6481 gccgggcgcc aggaagttcg ttacgatatt actaaaatgc attacatcgg ctcgcctgag
     6541 aaaagcgaca tcgtgctcta cgcgcgcgcc gatgcgccgt tcaaaacggt gcaagatatg
     6601 cgcaagctgc cggaaccggc caaatgcggc gccagcggca ccgcgagcgc ggattatctg
     6661 atcgccaaag ttctagacga cacgctcggc gtcaaaataa actcggtcct tggctacgcc
     6721 ggcggcagtg agatcgacat cgcagtcgag aaaggcgaag tgctctgccg cggcatgacc
     6781 attgcgccgc atttcggccg cgaacccttc gacagttggc acaagaaaaa tttcgaccgc
     6841 catatcgttc agaccgccga aaagcgcgac tcgcgcattg gcgacgtgcc gacgctgtac
     6901 gaaatttttg aaaaggaaaa cactgccgat gagagccgcc gggtcgccga tgtgattcta
     6961 cgcggcggcg attttggccg accgatgctg gtcggaccag gcacaccgct tgaccgggtg
     7021 aatatgctgc gcgaggctta cgtcaaatcg atgaataatc ccgagctcat cgcggaggcg
     7081 aaaaaaaccc gcatggacat ggaaccggtc accggtgaac agctgcaagc tctcgccaag
     7141 cgggtgatga atcagccccc cgccgtgctc aagcgcgtca aaaaacttct tgaataaaaa
     7201 tcatatcgtc acaagcgctg gtcagcagtc tcaagaaaag gagaactcat gaaagccgca
     7261 atcatattgc tcttcctcct cggcgccggg ccggcgcatt tggcagcgca gacgccatac
     7321 tatcaaggca agcaagttaa agtcattgtc ggcttcacta ccggcggttt ctatgaccgt
     7381 tgggcgcgga tactgtcgcg ctacatgccc aaacatattg ccggcaaccc ttcttttgtc
     7441 gtgcaaaaca tgcccggggc cggcgggctg accgcgacca attatgttta cagcgtggca
     7501 aagcccgacg gcacaaccat cggctttccg agcaacggac tctacctgga tcaattggtt
     7561 ggccgcaaag aagtgcagta cgacattcga aaatttgcct ggatcggctc gccggtgaaa
     7621 gaaccggcga tcttttatat gcgcgccgat acaccgttta aaaccattca ggatgtgaaa
     7681 aactctaagg aaccgcccaa gtgcggctcc accggcaccg cgagcaccga ttttatcttg
     7741 gcgcgcttgc tcgaggacac gctgccgccg ctgaagatca ccaccgtaca aggctatccg
     7801 ggcggcgcgg aaatcgatct agcggtggaa aaaggcgagg tcgcctgccg tggcatgacc
     7861 gcgtcgccat ttttcggccg cgaaccattt ctcagctggc aaaagaaaaa tttcgtccga
     7921 gtgctgctgt ttaccggcga caagcgcgat ccgcgcatgc ccgacactcc cacactcgat
     7981 gagatcttcg caaaagaaaa agtccccgaa gcgggacgtc gagtagcgga agtaattctc
     8041 gcagcagagt cctttggccg tccgattatc ggcacgccgg gcacgcccgc cgagagagtg
     8101 agtgaactgc gccgtgcgtt cgagcaggcg atgaaagatc ccgagttgtt agcagaagcg
     8161 caaaagcaaa agatggatgt cgatcccgtg tccggcgaaa atctcgagaa gctcgcgaca
     8221 agaatcctcg accaaccgcc cgaggtcttg gctcgggtga agaagatcct cggaaactag
     8281 ttgaataaac tcttgccgat gatagctttt agtcagcaaa ttgctttagg aggacgtttt
     8341 ttaatgttga aattactggc gtttttatcg gtctttatag ttggccaagt ggctcatgcg
     8401 cagacgccct tttatcaagg aaaaaccatt acaattgtcg ttggaaccaa ggccggcgac
     8461 gcctacgatt tgtatccgcg catgctcgcc gagttcatgc ccaaatatat tcccggcaat
     8521 ccaaatatta ttattcagaa tgtgcccggc gcagcgtcga tgatcgccgc caatcaagtt
     8581 tataacgtca gcaaaccgga cggtctgacc atcggcgcca tctaccctgc gctttatttc
     8641 gagcagctga cgaaaaaacc cgaagcgaag ttcgactgga ccaagttcgg ctgggtcggt
     8701 aatcccgtga catcgaatca tcttctttat atgcgctcgg atgctccgtt taagtcgatg
     8761 gatgatatca agaatgcgaa agaggcgccc aaatgcggct ccaacggtat cacctcgacg
     8821 ggttattacc tgccgaagct gatggaagaa accctgggca ccaaattcga tatcgtactg
     8881 ggttaccagt ccggccagga tgtcgacctc gcggtcgaac gcggcgaagt cgtgtgccgc
     8941 gcttttacca tcacggccta cttcgcccgg gagccgttca tttcctggcg caagcgcgga
     9001 ttcgtcaacg ccctgatgca gagcggttta aagcgtgaag cgcggctcaa ggacgccccg
     9061 acaatctatg aactgatgga taagtacaaa accaatgctg ccggcagaag cttggctaaa
     9121 gtcgtgctct ccgccggcga gtttggacgt ccgcttgtcg tcggccccgg cgttcccgcg
     9181 gatcgcttga aaatattgcg cgacgccttc gacaaatcgg tgacagatcc cgcgctcatc
     9241 gcgacagcgg aaaaacgccg cctggagatg gatcccgcgt ccggcgccga actcgaagca
     9301 ttggcgaaag aagtgatggc cgcgaccccg gacgtcgttc agcgtatgga gaaactcctg
     9361 ggcggcaaat aaaaattatg ccggacaatc cctcaagctg gttgcatcgc caccagcttg
     9421 agggatatgc tccggcgaat tttcggcggg aaggagaaac tttatgtttt caactctcgt
     9481 tgcgctggcc gctcttgcct ggagctcaac tcttttcgca caaacctctt tctatcaagg
     9541 caagaccatc accatcgccg ccggcaccaa agcgggcgat gtttacgatc tctatccgcg
     9601 actgctcgcc gaatacatgc ccaagcatat cccgggcggc cccaacatca ttgtgcaaaa
     9661 cattccggga gcggcttcgc tgatcgccgc caaccaagtc tacaataccg gtaaacctga
     9721 cggattgtct ctggcggcaa tctatccggc gctctatttc gatcagctga tcaagcgacc
     9781 ggaagtgaaa tacgactggg caaaatggca gtggctcggc agcccggtaa aatcgaacca
     9841 attgctctac atgcgcgccg acaccccgta caaatccatg gacgacatcc gtaaagcggc
     9901 gacggcgccc aagtgcggct ccaccggtat cgcttccgca ggctactaca tacccaagct
     9961 cttggaggaa gtgctcggct ccaagttcga tgtcgtatcg ggctaccaat ccgggcaaga
    10021 tatcgatctc gcggtggaac gaggcgagct ccactgccgg gcgtttacta tcaccgctta
    10081 ctatgcccgg gagccgttta tttcctggcg caagcggaat ttcaccaatg tgttggtcca
    10141 aaccggcagc cagcgcgatg cacgaatcaa agactccccg acgatttacg aattgatgga
    10201 caaatacaaa accagcggga cgggacgcac tctggcgaaa gtaatcctgg cgtcggggga
    10261 tttgggacgg cccatcgtcg ccccgccggg tgtgcctgcc gatcggatca aaatcttgcg
    10321 cgatgccttc gataaaacgg tcgccgaccc tgccttcctt tccgaagcgg aaaaacgccg
    10381 gctcgaaatc gatccgacaa aatgggatga gatggagtcg ctggcaaaag aagtcatgac
    10441 cacaccagcc gatgtggtgg cgcgcatgcg caagctcttg ggcgagtgag gacaattaat
    10501 agcttgacaa gcccgagggg gccggcgcgg cgtgcgattg gccgccggag atcgcataag
    10561 cgaaatagcg gaggccgctg catggttcca agcatcgtga ttttgctatc tgtgttactt
    10621 tggccaagtg gcggctatgc ccagactccc ttctataaag acaagaccat tagactcgtc
    10681 gccggcaccc cagcggggag cgtctatgac ctctacgcgc gcatggtcgc gcagttcatc
    10741 ggcaaataca ttcccggcaa tcccaatgtc atcgtgcaga acatgcccgg cgtcgcctcg
    10801 atggtcgccg ccaattacat ctacacggtc gccaagcctg atggtttgac catcggttcg
    10861 atccaaccag cgctctattt cgatcaactg gtcgggcgca aggaagttca gttcaattgg
    10921 caaaaattca cctggatcgg caacaccacg gtgtcgcact tattgctcta catgcgcggc
    10981 gacacaccgt ataagaccat agacgacatc cgcaaagcgg ccgtgccgcc caaatgcggc
    11041 gccgaaggga cggcatcgtc ggcctactac ctgccgaggc ttctggaaga aaccatcggc
    11101 gcgaaattca atgtagtcct cggttacaac tccggcaccg atgtcgattt ggccgtggaa
    11161 cgcggcgagg tgcagtgccg cgcctttacg gtcgccgcgt tcttcgcgcg cgagccgttt
    11221 atcacctggc gcaagaaagg ctttgttcgg gtcatcgttc agaccggcaa acaacgcgac
    11281 tccaagctgc ccgacgtacc cgcactccaa gaactgatgg agcaaaataa atccgccgaa
    11341 gcgagtcgcc gtctggcggc cgtgctctta gccgccaacg agttcggccg gccgatcatc
    11401 gcctcgcccg ggattccggc cgaccgcgtg aagattctcc gcgaggcatt tctgaaaacc
    11461 atcaccgatc ccgatctctt ggaggacgcc aaaaagaaac ggctcgacct cgatccggtt
    11521 tcgggcgaag aactggaaag cctcgccaag gagatcatgg tgcaaccgcc cgacgtcatc
    11581 gaacgcatga aaaaattatt gggcacgtag aatcaaccgt cagcactcag gtgaacaatt
    11641 aaaggagaaa cgcacatgat tagagccgca ttcgtccgtt cggtcctatt cgtactattg
    11701 gcgctattat tctcgacccg actgcatgcg cagacgccat tttatcaagg caagacgatc
    11761 cagatcatcg tcggcgccaa ggcgggcgac acctatgacc tttacccgcg tctggtcgcg
    11821 gaattcatga gcaagcatat tccaggcaat ccgaacatca tcattcaaaa cgtcgctggc
    11881 gctgcgggcc tcatcggcac caatcaggtt tacaatctcg ccaaacctga cggtctaacc
    11941 ctcggcgcga tttatcctgc gctctatttc gagcagctcg ccaaacggcc cgaagccaaa
    12001 ttcgactgga ccaagttcat ttggatcggc agcacggtga gctccaacag ttacatgtat
    12061 atgcgcgctg ataccccgtt taaaaccatc gaagatgtcc ggaccgcggc gactccgccg
    12121 aaatgcggtg caacgggcgt aacgtcgtcg gcctattaca tgccgaaact cctggaagag
    12181 gctataggca caaagttcga gattgtctcg ggttatgtcg ccgggtcgga tatcgacctc
    12241 gccgttgagc gcggcgaagt tcagtgccgg gcctttacga tcaacgcctt cttctctcgc
    12301 gagccattta tcacctggcg caagaaaaat ttcgtgcgcg tgctctatca gaccggcagc
    12361 aaacgcgatg cacggctcaa ggacgtgccg ctgctcactg aactgatgga caaatacaag
    12421 acccctgaaa acgtccggcg actagccaaa gtggtcctcg ccgccgat
//
LOCUS       DATLCH010000255         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10032950, whole genome shotgun sequence.
ACCESSION   DATLCH010000255 DATLCH010000000
VERSION     DATLCH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10032950"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1389)
                     /locus_tag="VMZ02_07530"
     CDS             complement(334..1389)
                     /locus_tag="VMZ02_07530"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUR71820.1"
                     /translation="MKAAVLYEVRQPLKIEDLEMPDVHDDDVLIKVVSCGVCHTDLKV
                     IEGRNRFTAPTILGHEVAGTVEAVGAQCKDFKVGDRVIIGMRYKCGRCRYCLSAHENL
                     CERRPAPPNLKKKDGTVVTRWNVGGFAQYVSVPAYMIFKIPDGISMEEASVVGCRVTT
                     AYNAVKHRGKLEPNESALVIGCGGIGLNTIQFLTCFGAYPIIAVDIADEKLAAAKDFG
                     ATHTINANADDPVKAVIKLTGGGVDKAFEAIGNAKTSDQIIQATRPGGTATIIGGLGS
                     GPFTISSGNFVTREITITGVSSRQANDVDEVFQMAQQGRIELKSLITQNYRYEQINEA
                     LHDLEHGKLRMGISLWN"
     gene            complement(1524..2225)
                     /locus_tag="VMZ02_07535"
     CDS             complement(1524..2225)
                     /locus_tag="VMZ02_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HUR71821.1"
                     /translation="MLTLALLIGAAPQFGFAQAPGASSPTKIGIIGSGNVGSTLGEFW
                     VKAGHEVLFSSRNPENLKELVAKIGPRARAGTVSEAIAFGEVILIAVPYGSMAQIAKD
                     YAKDLAGKIVLDTGNAVLARDGEIGKEAREKGVGLTTQRLLPGARVVRAFNTLGVGRL
                     RSNANRAETRMAIPMAGDDPEALRVASMLVRDAGFEPAVLGGLDRSRIFEQTNPLYGQ
                     ELSAPEMIERAKAIK"
BASE COUNT          498 a          709 c          634 g          562 t
ORIGIN      
        1 gtattgggcg ttaaaaatat gctgcacaac gccgggttcc tggttgtagg cttcttcgat
       61 tagatagcta ttgtcctcga tgacatcgat cggcctttcc tctgccgcgt tagcgctgct
      121 ggagacgagc cacacgagca cggcaactag tgcgccgcag cattttatat ttcgcattat
      181 gccttcgctg taatcatact gtcgaaagta gcgcaactcg agcttctgtt gaaccgccgg
      241 cttggactta tgcgaacgtg ctgaagtatc cgaagacgat ttgccccgtt cgaacatcag
      301 tgttctcgaa tcttgaagcg cgcgttcttc acgctaattc cacagcgaaa ttcccatcct
      361 caacttgcca tgctccaagt cgtggagcgc ctcgttgatt tgctcgtagc gatagttctg
      421 cgtgatcagg cttttcaatt caatgcgccc ttgttgggcc atttggaata cttcgtcaac
      481 gtcgttggct tgccggctcg acacaccggt aatggtgatt tcgcgggtta cgaagttgcc
      541 gctgctgatc gtgaacggcc cgctgcccaa accgccaatg atggtcgccg tgcccccagg
      601 ccgggttgcc tgaatgatct gatccgaggt cttggcgttg ccgatcgctt cgaaggcttt
      661 gtcgacgccg ccgccggtca gcttgatgac cgctttcacc ggatcatccg cgtttgcatt
      721 gatcgtgtgc gtcgcgccga agtctttcgc cgcggctaat ttctcgtcgg cgatatcaac
      781 cgcgatgatc ggatacgcgc cgaagcatgt gagaaattga atcgtattga ggccgatgcc
      841 gccgcagccg atcacgagag ccgactcatt gggctcgagt ttaccgcgat gcttgacggc
      901 gttgtacgcg gtggtcaccc ggcagccaac cacgctcgct tcttccatag aaataccgtc
      961 ggggattttg aatatcatgt acgccggcac cgagacgtac tgggcaaagc cgccgacatt
     1021 ccagcgggtc accacggtgc cgtctttctt ttttaaattc ggcggcgccg gccggcgctc
     1081 gcagaggttt tcgtgagcgc tcaagcaata gcggcagcgg ccgcacttgt agcgcatacc
     1141 gatgatcacc cggtcgccga ctttgaagtc cttgcactgg gcgccaacgg cttcgacggt
     1201 gcccgcgact tcgtggccga ggattgtcgg cgcggtaaat cgattacggc cctcgatgac
     1261 ttttaagtcg gtgtgacata caccgcagga cacgactttg atcagcacat cgtcatcgtg
     1321 cacatcaggc atttcgagat cttcgatctt tagaggttga cgcacttcat agagaacggc
     1381 ggctttcatg aggactcctt tcgggtcacg gcaaaagaaa gggcaaccac agggttgccc
     1441 cgacaaatcg tcttgcaaat aattacgctg ctagaccgcc tacggtcaag ggaaagaaca
     1501 cttgtgaatc ggctttgcct actttacttg atcgccttag cccgctcgat catctccggg
     1561 gctgagagct cctgaccgta gagcggattg gtttgctcaa atatcctgga acgatcgagt
     1621 ccacccagca ccgccggttc aaaaccggcg tcacgcacga gcatggatgc gactctcaac
     1681 gcttctggat cgtcgccggc catcgggatg gccatgcggg tttccgcgcg attggcattg
     1741 ctcctgagcc gaccgacgcc caaagtattg aaagcgcgta ctacacgcgc gccgggaagc
     1801 agtctttgcg tggtcaaacc gacgcccttc tcgcgcgcct ctttgccgat ctcaccatcg
     1861 cgcgccaaga ctgcgttgcc ggtgtccaaa acaattttcc cggccagatc cttggcgtaa
     1921 tcttttgcaa tctgtgccat cgagccgtag ggaacggcga tcagaatcac ttcgccaaat
     1981 gcgattgcct cgctcaccgt accggcgcgc gcacgcggac caatttttgc caccagctct
     2041 tttagattct ccggattgcg cgatgaaaac agtacttcat gccccgcctt cacccaaaac
     2101 tctcccagtg tgctgccgac gttgccggac ccgatgatgc cgatcttagt cggcgacgac
     2161 gcacccggcg cttgggcaaa gccaaactgc ggcgcagcgc cgatcagcag ggccaacgtc
     2221 aacatgagcg atagaccttc ttgaaatgag cgaggaatcc atctgcgcaa agcaagtctt
     2281 gtcataaccg gtatccccta tacacactac gaactccgct acggtttgaa cgtcttgaac
     2341 ggcttgaacc ttttgaacat gttcatcgcc gattaacgcg gatacagctc cttgataaac
     2401 cca
//
LOCUS       DATLCH010000256         9804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_691502, whole genome shotgun sequence.
ACCESSION   DATLCH010000256 DATLCH010000000
VERSION     DATLCH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691502"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1132)
                     /locus_tag="VMZ02_07540"
     CDS             complement(116..1132)
                     /locus_tag="VMZ02_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71822.1"
                     /translation="MLFGMALVVVGLFLQGVSHGAEIIGNDLIEKAKAEGEVVWYSTL
                     PLPETQAWAALFTKKYGIKINLFRAGATAMTQKLQTEIPAGRWGFDVVMNRASNLELY
                     RRKNWLQKMNYPERSRFLDGFMDPDDYWAAYYIQFWSVAYNTHMVKPNDLPKSYEDLA
                     SAKWKDKLTMDEQDVDLYATWLKLMGRERGREWFKKLVANGLRPRRGHTMEAQLVSAG
                     EFALGISAYASVIENIRAKGAPIDWVAFEPVPVNVLAWGIGARPPHPNAARLFLYWGL
                     SAEGLKAMNEISDRVPARRDVPIKNKRLERMMQAKLAPVDISIADRYTELNQEFLSLL
                     GLKY"
     gene            complement(1148..2305)
                     /locus_tag="VMZ02_07545"
     CDS             complement(1148..2305)
                     /locus_tag="VMZ02_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR71823.1"
                     /translation="MKSAYGLKIPEADPELTRVGAGTPCGELMRRYWQPVCLSADLKD
                     LPKKVKILGEELIAFRDGRGTAGLLFFRCSHRGSSLEYGRVEERGLRCCYHGWLYDVE
                     GNVLEMPLEPSPNAFLKQIQHPVYPIREFGGLVFAYMGPPERMPEFPLYDIWQRPGGT
                     LKARMGPRVGGPVDCNWLQAEENLMDALHTFWLHTLHSGPQFPSTAYGANPDDLRYEE
                     TQMGMRFVLARKLASGKWWELIWEMIMPLNVHLVYTDEPKSERVRAVTYCVPTDDTHQ
                     LGASVRWLADDESESLSGREQLAPAGRQDRSLDYIQRHPDDKEAVEGQGPIALHGLEH
                     LVSSDKGVLLFRKILRTAIHEVREGRDPKGILRDAASAGNVHTSAGSVVRD"
     gene            2451..3551
                     /locus_tag="VMZ02_07550"
     CDS             2451..3551
                     /locus_tag="VMZ02_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71824.1"
                     /translation="MAHSFPIIDADAHVIETERTWDYLNDAEAKYRPRLYSTPNDPTR
                     QYWVLEDKIAGFRFLTLNERELQEFADRAGRNFATPQAARELDDVELRLKHMDELGID
                     VQVLHNTFWIEQVTTKPQAEVAVCRSWNRWLADISKKSHGRLFYSCVIPAMDIDEAID
                     EIKFAKENGGVAVCMRPLEGDRHLSDPYFYPIYQAASDLDLAIAVHIANGNPENADLY
                     RQAPAGRFAQFRVPTVTACFDVIMSDLHQVFPKLRWGFIEASAQWVPWIARETAIRYR
                     AQGRAFPENVFGEYNIYVTCQTNDDVPYVVNCCGENRLVIGTDYGHTDPSSAITALNE
                     FQRMEGLEARVKEKILSHNAKALYNLSANGVH"
     gene            complement(3914..5200)
                     /locus_tag="VMZ02_07555"
     CDS             complement(3914..5200)
                     /locus_tag="VMZ02_07555"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HUR71825.1"
                     /translation="MLFQKQLRDFVNEIFAGLPMGAAFCAVLVLIALTFVPVSNPSSN
                     DETNSLLRPAKNSSEERIAVKLGRSESLQGFLRRIGLRPGSAQELLQKMYAAVDLRKM
                     PRDQTFNVIIGPQDQDIRAVEFVLQDHLVRVSASLAGWSVERQELAHVAGLNNVRFRI
                     TDNFVRSAVRAGVSNAQIAQLQRIFSAEVDLSTDLAAGDEVLLLLPEKQYLDGHIVQS
                     PMTAVRVVHGERLFEAFGFTGASGVLQYYDSDGQLLPRAFLAAPLAFERISSTFALAR
                     PDPATGALRPHEAIDFQAPHGTPVMSVGSGTVEFAGARGGYGLMVELKHAGGYTTSYA
                     HLSRIADGIEEGRRVKAGDALGEVGQTGYATGPHLHFEFARDGEKLDYLSTKLPSAES
                     LTGFRLLQFKLEQAKWRSALRGTAVRIVQTPVSTWQ"
     gene            complement(5487..6428)
                     /locus_tag="VMZ02_07560"
     CDS             complement(5487..6428)
                     /locus_tag="VMZ02_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HUR71826.1"
                     /translation="MAKYRENLPQLKGDLFLTDGGIETTLIFNEGLDLPYFAAFDLLK
                     HTAGQAALRKYFQSYSAIARKYSVGLILESATWRASSDWGEKLGYSHDELVAANRQAM
                     AILHEVRREYETEQTPMVISGCIGPRGDGYNPASFMSEAEAEYYHGMQTKLFRDVDAD
                     LVTAITMTYPEEAIGIVRAGQSVGMPVVISFTVETDGRLPNGQSLHDAIAQVDRATGN
                     APVYYMINCAHPSHFTDRLDNGAPWLRRIRGLRANASPKSHKELDEATSLDAGDPADL
                     ANRYRALMAQLSNLNVVGGCCGTDPRHIEAIYRAVRS"
     gene            complement(6451..7431)
                     /locus_tag="VMZ02_07565"
     CDS             complement(6451..7431)
                     /locus_tag="VMZ02_07565"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71827.1"
                     /translation="MPLVSIRLAALLLLFALAPNIEAQSTKLRAAYSAESSWSLATWI
                     ALEGGLYKKYGLDVDLVLIRSASTITSALIAGEAPMIQLGGNGTIQAGLQGADTVNIL
                     TLVPLIPQSLVVTPDINSAADLRGKRLGVSRFGALSDLVIRRYLRKLGLDPAKDVTIV
                     QIGGIPELVTAMKAGAISGGSISPPALTVAKKAGFRELSDFESLDYKYPAVTIATTRS
                     FIQRSRPIALNFIRGEIEGIHAIRTQKSLAINVLKKYMRINDPEILEDGYRYAVKFFQ
                     ARPYPTLDEARAVLEEYKSMPQAANAKPEQFIDLTLLQDLEKEKFFDKLK"
     gene            complement(7714..8721)
                     /locus_tag="VMZ02_07570"
     CDS             complement(7714..8721)
                     /locus_tag="VMZ02_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71828.1"
                     /translation="MREPAPALRRELMTMKRGLGIICLVVIWRFIASAYAAQSDPTKL
                     TVGYSPISAATLPFFIALEERLFQKHGLEVVPVFFGGSPLINAAIMAGEFPIGLTGGA
                     GIISSQLAGSDLTVIGSYLQVLTIDGMAKPEIKSINDLKGKKVAVSRIGASTYFAGLS
                     MLDSRGMKPTDVVFIQAGGVGESFAALVNGAVDAAMIGYPFGLKAKQAGFQILFRPPD
                     TEYGLYPNATVAARESWLKEPRNRKIAVTFLRAMNEGLHLAKTDAVASKKALRRFTRV
                     QDEATLQGTFEFFQPYFPTTLRTVEKAMANAIKFLDHPKAKQLDPKQTFNNSYVDDAL
                     K"
     gene            complement(8685..9779)
                     /locus_tag="VMZ02_07575"
     CDS             complement(8685..9779)
                     /locus_tag="VMZ02_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71829.1"
                     /translation="MPGVLDADTHIAESEAMWSFIEKEMYPRRPVLARIPDDTWYGER
                     NAFWLIDGEIFPKPAGKASFSLITPSAQKKESGRGDIHLAVRELTDPLARLRDMDKIG
                     VDVQVIYPTLFLVYLTDDAQLDVALCRAYNRFVANACAKAPDRLKWAVILPLRDIDAS
                     LEEMKWGKQQGAVGVFFRGMEGNLTLDNPQLHPIYALAEKLDLTLCIHTGSGSRHLMQ
                     LFDVERNHTFAHNRVLPVIAFRDLVANRIPELFPKLRFGFIEAAASRVPYIWHVLRRS
                     VRPDFKTKNPQDLFREYRFWIACEADEELPHLLNYIGEDHLVIGSDYGHNDPSKEPDF
                     VRNMRARKDVAPKIIEKILCENPRRLYAVS"
BASE COUNT         2231 a         2830 c         2605 g         2138 t
ORIGIN      
        1 tgatggtaag tccgtaatgc cggtgaaaac cggcatccat cttcgttttg actcgccagt
       61 cagaaactgg attccggcgt gcgccggaat gacagtaaaa aagaatagca ttccctcagt
      121 actttaaacc gagtaacgag agaaactcct gattcagttc cgtgtaacga tcggcgattg
      181 aaatatccac cggtgcaagt ttggcttgca tcatgcgctc gaggcgcttg tttttgatcg
      241 gcacgtcgcg gcgcgccggc acccgatcgg aaatttcgtt catcgctttg agcccttcgg
      301 cggataatcc ccaataaaga aacagccgtg ccgcgttcgg atgcggcggc ctagcgccga
      361 tgccccacgc caggacattc accggcactg gctcgaaagc gacccaatcg atgggcgcgc
      421 ccttggcgcg aatattttcg atcaccgaag cgtaggcgct aatgccgagg gcaaactcac
      481 cggcgctgac aagttgcgct tccatcgtat ggccgcgtcg cggccgcaat ccattggcga
      541 cgagcttttt aaaccattct cggccacgct cgcggcccat cagtttgagc caggtggcgt
      601 agagatcgac atcttgttcg tccatcgtca gcttgtcttt ccacttggca ctggccaaat
      661 cctcgtaact tttcggcaga tcgttgggct tgaccatatg ggtgttgtag gccaccgacc
      721 agaattgaat atagtaagcg gcccaataat cgtccggatc catgaagccg tcgagaaagc
      781 ggctgcgctc gggataattc atcttctgca gccaattttt gcgccggtat agttcgagat
      841 tgctggcacg attcatgaca acgtcaaatc cccagcgacc tgcagggatt tcggtttgca
      901 gcttttgggt catcgccgtg gcgcccgcgc gaaacaagtt gatcttgatg ccgtactttt
      961 tagtgaacag cgcggcccac gcctgggttt ccggcagcgg caaggtcgaa taccaaacca
     1021 cttcgccttc ggctttggcc ttctctatca gatcgttgcc gattatctcg gcaccgtgcg
     1081 acacgccctg aagaaacagt ccgacaacca ccagtgccat gccaaacagc attgattttc
     1141 ctccaactca gtcacgcacg acgctgccgg cgctggtgtg aacattgccg gcgctcgcgg
     1201 catcgcgcag gatgcctttc gggtctcgtc cctcgcgcac ttcgtgaatc gccgtgcgca
     1261 agatctttct aaacaaaagg acgcctttat ccgaactgac cagatgctcc aagccatgca
     1321 gcgcgatcgg cccctgcccc tcgactgctt ctttgtcgtc ggggtggcgt tgaatgtagt
     1381 cgaggctgcg gtcctgccgc ccggccggcg ccagctgctc gcgtcctgac aacgattcgc
     1441 tttcgtcatc ggcgagccaa cgcacgcttg cacccaattg atgcgtgtcg tcggtcggca
     1501 cgcaataagt cacggcgcgc acgcgctcgc tcttgggttc gtctgtataa accagatgga
     1561 cattgagcgg catgatcatt tcccagatga gttcccacca cttgccgcta gccagtttac
     1621 gcgcgagtac aaagcgcatg cccatctgcg tttcttcgta gcgcaagtca tcgggattgg
     1681 cgccgtaggc agtcgaggga aattgcggcc cgctatgcag cgtgtgcaac caaaacgtgt
     1741 gcagcgcgtc cattaaattc tcttcagcct gcaaccagtt gcaatccacc ggaccgccga
     1801 cccgcggccc catgcgcgct ttcagcgtac cgccagggcg ctgccaaatg tcataaagtg
     1861 gaaattccgg catgcgctcc ggcggcccca tgtaggcgaa gactaaaccg ccgaactcac
     1921 ggataggata aaccggatgt tggatttgtt tgagaaaggc attcggcgac ggctccagcg
     1981 gcatctccag gacattacct tcgacatcgt agagccagcc atgatagcag cagcgcagac
     2041 ctcgttcttc gacccggcca tattcaagcg acgaaccgcg atgactgcag cgaaaaaaca
     2101 gcaaacccgc tgtgccccgt ccatcgcgaa aggcgatcaa ctcttcgccg aggattttta
     2161 cttttttagg taaatctttg agatcagcgg aaagacagac cggttgccaa taacggcgca
     2221 tcagctcgcc acagggcgtg ccagctccga cccgcgtcaa ctcaggatcg gcttcgggaa
     2281 tcttgagacc gtaggcgctc ttcatctgct ggtcctctgg ctaaacgtca tcgcggcgat
     2341 catatgccgt cgtcagtgcc agtcaaggta atacttatgc agagcttgac agtgtcgcgc
     2401 gatccgcact agtttcggtc attcttttgc agccgaaggg agaccttcca atggctcatt
     2461 catttccaat catcgatgcc gacgcccatg tcatcgaaac cgaacgcacc tgggattacc
     2521 tgaatgacgc cgaggccaag tatcggccgc ggctctattc cacgccaaac gatccgaccc
     2581 gccagtattg ggtattggaa gataagattg ccggatttcg atttttgacg ttgaacgagc
     2641 gcgagctcca agaatttgcc gaccgcgccg gcagaaactt cgccacgccg caagcggcgc
     2701 gagaactgga cgatgtcgag ctgcgtctca aacacatgga cgagctaggc atcgacgttc
     2761 aggtgttgca caacactttc tggatcgaac aagtgacgac caaaccccaa gccgaggttg
     2821 ccgtgtgccg cagttggaat cgctggcttg ccgatatttc gaagaaaagt catggccgcc
     2881 tgttctactc ctgtgtcatt cccgcgatgg acatcgatga agcgattgac gaaatcaagt
     2941 ttgccaaaga aaacggcggc gtcgccgtgt gcatgcgccc cctcgaaggc gatcgccatc
     3001 tgtccgatcc ttatttttat ccgatctatc aagcagccag cgatctcgac ttggcgatcg
     3061 cggtgcatat cgccaatggc aatccggaaa acgctgacct ctatcgccag gcgcccgccg
     3121 gccgtttcgc gcagtttcgc gtgccgacag tgacggcctg tttcgacgtt attatgagcg
     3181 acctgcacca agtgtttccc aaactccgtt ggggcttcat cgaagcatcg gcacaatggg
     3241 taccctggat cgcccgcgag acagcgattc gttaccgcgc ccaaggacgc gcgttccctg
     3301 agaatgtctt cggcgaatac aacatttacg tcacctgcca aaccaacgac gacgtgcctt
     3361 acgtggtcaa ttgctgcggt gaaaaccggc tggttatcgg taccgactac ggccataccg
     3421 acccgtcgag cgcgataacc gcgctcaacg agtttcaacg aatggaaggt ttagaagcgc
     3481 gagtgaagga aaagattttg tcgcacaacg ccaaggcgct ctacaatttg tcggcaaacg
     3541 gggttcatta agtggtgagg aaaacaaaaa cttcaccacg aagcgcacga agtgtcggat
     3601 aattgtaatt ccgggcttcg tgcgctttgt gtcatccttc gaaaatacat gagcaggtac
     3661 agtctgggcg acgaatccac atgaagcttg tgtcataccg gcgaaagccg gtatccatcc
     3721 cctccggccc ctggatgccg gccttcggcg catgacgaat caaagggaag aaccatatga
     3781 tggaaagaag gctacaatct catagtcggt agccgagcgt atgcccctgg actagtcgtg
     3841 gtgagaatga atttctaatt gaactctccg tgtaacgctg cgcgctagac gtcgcgcgaa
     3901 ctcgaacgct gcgctattgc caggtcgaga caggcgtttg gacgatacgt acggccgtgc
     3961 cgcgcagtgc cgatcgccac ttcgcctgtt cgagtttgaa ttgtagcaat ctaaatcccg
     4021 tcagcgattc tgcactgggg agttttgtcg ataggtaatc gagtttctca ccgtcgcggg
     4081 cgaattcgaa gtgcagatga ggcccggtgg cataaccggt ttggccgact tcaccaagcg
     4141 catcaccggc tttgacgcgg cgaccttcct cgatcccgtc ggcaatgcgg gacagatgcg
     4201 cataactcgt tgtgtagccg ccggcatgtt tcaactcgac catgagtccg tatccgccac
     4261 gagcaccggc aaattccacc gtacccgatc cgacagacat cacaggcgtt ccgtgcggcg
     4321 cttgaaaatc gatcgcctca tgaggccgga gcgcaccagt ggccggatcg ggacgcgcta
     4381 aggcgaacgt cgatgaaatc cgctcgaacg caagtggcgc cgccaaaaag gcccgcggca
     4441 gcaattgccc gtcggagtca taatattgca gaacgccact agcgccggta aagccgaacg
     4501 cctcgaataa cctttcgcca tgcacgactc ggaccgcagt cattggactt tggacaatat
     4561 gtccatccag atactgtttc tccggcagga gtaacaatac ctcatcacct gctgccaagt
     4621 cggtcgacag atccacttcc gcactgaata ttcgttgcag ctgggctatc tgcgcattgg
     4681 acacgccggc acgaacggcg ctccggacaa aattatcggt gattcgaaac cgaacgttat
     4741 tcaatcccgc cacatgagcc agttcctgcc gttcaaccga ccagccggct aagctggcgc
     4801 tgacacgcac caggtggtcc tgcaaaacaa attcaacggc acggatatcc tggtcttgag
     4861 gaccgatgat tacattgaag gtctgatctc gcggcatctt gcgcaaatcg acagccgcat
     4921 acattttctg caacaactcc tgggcggagc cgggacgaag gccgatacga cgcaaaaagc
     4981 cctgaagcga ttcgcttcgg ccgagcttaa ctgcgatccg ctcttcgctg ctattcttcg
     5041 caggtcgcaa taacgaattg gtttcgtcgt tgctcgacgg attggacacc ggcacaaatg
     5101 tcagtgcgat caaaacgagc acggcgcaaa acgccgcccc catcggcagt cccgcgaaaa
     5161 tctcgttaac aaagtctcgt agctgttttt gaaaaagcat acgtaggaag gaaatctccc
     5221 gagatcaact ctttaaagtg taccttttag aacacaaact ggaagacaac ccgcaaagtt
     5281 ttttttcgaa atcgacgtaa acaaaattat gtgcagcgcg caaagcgcga tgataattaa
     5341 ccttgccgga caatgtgtga ctgtaaagga ccgggaacga cgccggcaaa cgccgctgag
     5401 acgacacaac gaaactgccg cgctttcgga gaattcttcc gagctaatca caaacttcgg
     5461 ccatagcatt ctcgctgccc cgagattcac gagcggaccg cccggtagat cgcttcaata
     5521 tgacgcggat cggttccaca gcagccgcca accacattca gatttgacaa ctgcgccatc
     5581 agggcacgat atcggttggc aagatcggcg ggatcgccgg catccagact cgtggcttca
     5641 tcgagttcct tgtggctctt gggcgatgca ttggctctga ggccgcggat tcgcctcagc
     5701 catggcgcgc cgttatcgag tcgatcggta aaatggctcg gatgcgcgca attgatcatg
     5761 tagtagactg gagcattgcc ggtggcgcga tccacctggg caatggcatc atgcaagctt
     5821 tgaccgttgg gcagccgtcc gtcggtttcc actgtgaatg aaatcaccac gggcattccg
     5881 accgactgtc ccgcgcggac aatgccgatc gcttcttcgg gataggtcat ggtaatcgcg
     5941 gtaaccagat ccgcgtcgac atcacgaaat agtttagtct gcatgccgtg atagtattcc
     6001 gcctcggctt cgctcataaa gctcgccgga ttataaccgt cgccgcgcgg cccgatgcag
     6061 ccgctgatca ccatcggcgt ctgctcggtc tcatattcgc gccgcacttc gtgcaaaatt
     6121 gccatcgcct gccggtttgc ggccacaagt tcgtcgtgag agtagccgag tttttcaccc
     6181 caatcggaac tcgcccgcca ggttgcgctt tccaatatca atccaacgga atacttgcgc
     6241 gcgattgcgc tgtagctttg aaaatatttg cgtagcgcgg cttgcccggc agtatgcttg
     6301 agcagatcga acgccgcgaa atacggaaga tcgagcccct cgttaaatat cagcgtggtt
     6361 tcgatgccac cgtcggtgag aaacaaatct cctttgagct gtggcaaatt ttctcggtac
     6421 ttcgccattc gaccacctta ataagaagcg tcatttgagt ttatcgaaaa atttttcttt
     6481 ttctaaatcc tgcaacaagg ttaaatcgat aaactgttcc ggttttgcgt tagctgcctg
     6541 cggcatgctc ttgtactctt cgaggacggc acgcgcttcg tcgagcgtcg gataaggccg
     6601 ggcttgaaaa aacttgaccg cataacgata gccgtcttcg aggatctccg gatcgttgat
     6661 gcgcatgtat ttttttaaaa cattgatggc aagactcttt tgcgtgcgga tggcgtgaat
     6721 accttcgatt tcgcctcgaa tgaaattgag cgcgattggc cggctgcgct gaatgaaact
     6781 tctcgtggtc gcgatcgtca ccgccggata tttgtaatcg aggctctcga agtcggagag
     6841 ctctcggaag ccagccttct tcgccaccgt caacgctggc ggtgaaattg acccgccgga
     6901 aattgcgccg gccttcatcg ccgtcaccaa ttcggggatg ccgccgatct gcacgatggt
     6961 gacatctttc gccggatcga gcccgagttt gcgaagatag cggcgaatga cgagatcgct
     7021 gagcgcgccg aaacggctca cgcccaaacg tttgccgcgc agatcggcgg cagaattgat
     7081 atcgggcgtc accaccaaac tttggggaat aagcggcacg agtgtaagta tgttgactgt
     7141 gtccgccccc tgcaaaccgg cttggatggt gccgttgccg ccgagttgaa tcatcggcgc
     7201 ttcgccggca atcaacgccg aggtgatcgt cgacgcgctg cgaatcagaa ctagatcgac
     7261 atcgaggcca tacttcttat acaagccgcc ttcgagagcg atccaggtcg caagcgacca
     7321 ggaactctcg gccgaatagg cggcacgcag cttggtcgat tgcgcctcga tattgggcgc
     7381 cagcgcgaac agcaggagca aagctgcaag acgaatgctt accagtggca tattccctcc
     7441 ttgttaaaac tcttcctccc aggtgacttc gatcaacgtc aactgaacgg ccctcggctc
     7501 aaaacagaca gatctaaatt catctagtgg aagtcgacac tttctttcgt cattccggcg
     7561 aaggccggaa tccagggttt cttttgcctt caaaaccgaa gatggatgcg gccttcgccg
     7621 gcatgacaaa ttctcttttc ttcggaagtc gtaggatttc aaccaatccc cgaaagcaac
     7681 atccatttaa tgcgtccatc gctcccaccg aacctacttc agcgcatcat caacataact
     7741 attgttgaac gtctgctttg ggtcgagttg tttagctttg ggatgatcga gaaatttgat
     7801 cgcgttggcc atcgcttttt cgacggtccg cagagtcgtc ggaaaataag gttgaaagaa
     7861 ttcaaaggtg ccctgaagcg tcgcctcgtc ttgaaccctg gtgaagcgcc gcaacgcttt
     7921 tttgctggca acggcatcgg tcttcgccaa atgcaaaccc tcgttcatcg cgcgcaaaaa
     7981 ggtcacggca atcttacggt tgcgcggctc cttcaaccaa gactcgcgcg cagctaccgt
     8041 ggcgttggga taaagcccgt actcggtatc gggcggtcga aacaggattt ggaatccagc
     8101 ctgtttcgct ttcagcccga acggataacc gatcatcgcg gcatcgaccg cgccgttgac
     8161 cagagctgcg aaagattcgc cgacaccgcc ggcctgaatg aagacgacat cggtcggctt
     8221 catgccgcgc gagtcgagca tcgacagccc ggcaaaatag gtcgacgcgc cgatccggct
     8281 gacggcgact ttcttgcctt tcaaatcgtt aatcgacttg atctcgggct tggccatgcc
     8341 gtcgatggtg agcacctgca aataactgcc gatgaccgtg agatcgctgc cagccaattg
     8401 gctggaaatt attcccgcgc cgccggtgag accgatggga aactcgccgg ccatgatcgc
     8461 ggcattgatc agcggcgagc caccgaaaaa caccggcacg acctccaagc catgtttttg
     8521 aaacagccgc tcttcgagag cgataaaaaa cggcagcgtt gccgccgaga tcggcgagta
     8581 gccgaccgtg agcttggtgg gatcggattg cgcggcatac gccgacgcta tgaaccgcca
     8641 aatgacgaca aggcagatta ttcccaagcc gcgtttcatc gtcatcagct cacggcgtaa
     8701 agccggcgcg ggttctcgca taaaatcttc tctataattt ttggcgcgac atctttccgc
     8761 gcgcgcatat tcctgacgaa atcgggctcc ttggacggat cgttgtggcc gtaatcggag
     8821 ccgatgacga gatggtcttc gccgatatag ttgagcagat ggggcagctc ttcgtcggct
     8881 tcgcaggcga tccagaagcg gtattcgcgg aacagatctt gcgggttctt ggttttgaaa
     8941 tccggccgca ccgagcggcg caacacatgc cagatgtacg gcacccggct cgccgccgcc
     9001 tcgatgaaac caaaacgcag cttgggaaac agctcaggta tccggttcgc caccagatcg
     9061 cgaaacgcga ttacgggcag gacgcgatta tgggcaaagg tgtgattgcg ctcgacgtcg
     9121 aagagctgca tcaagtggcg gctgcccgaa ccggtgtgaa tgcacagcgt caaatccaat
     9181 ttctctgcca acgcatagat gggatgaagc tgcgggttat cgagcgtcag gttgccctcc
     9241 atgccgcgaa aaaacactcc caccgcgcct tgctgcttgc cccatttcat ctcttccaac
     9301 gatgcatcga tatcgcgtag cggtagaatc accgcccact tgagccgatc cggcgctttg
     9361 gcgcaggcat tagcgacgaa gcgattgtaa gcccggcaaa gcgccacatc cagctgcgcg
     9421 tcgtcggtta gatagactag aaacagggtc ggataaatca cctggacgtc gacgccgatc
     9481 ttatccatat ccctcaagcg cgccagggga tcggtcaact cacgcaccgc cagatggatg
     9541 tcgccgcggc ctgattcttt tttctgcgct gacggcgtta tcaaactgaa actcgccttg
     9601 cccgcgggct tagggaaaat ctcgccatcg atcaaccaaa atgcattgcg ctcaccgtac
     9661 caggtatcgt cgggaatccg cgccagcacc ggtcggcgcg gatacatctc cttctcgata
     9721 aaactccaca tcgcttctga ttcggcgata tgggtgtcag catctaaaac tccgggcatc
     9781 gtaactccct aaataaaact cgtt
//
LOCUS       DATLCH010000257         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5158146, whole genome shotgun sequence.
ACCESSION   DATLCH010000257 DATLCH010000000
VERSION     DATLCH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5158146"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..912
                     /locus_tag="VMZ02_07580"
     CDS             364..912
                     /locus_tag="VMZ02_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HUR71830.1"
                     /translation="MRRLGGLFSSTLLVGLLLGTGTASALPGDGASEVQVSAGFNQTQ
                     GSDTGSLNVDLSYGYYLTPGWQLGFRQAINSTFVDNGRDFWLATTTPFLNYNFRLTNI
                     IVPYLGAFIGAAWNDRDITGTAGPQVGIKFFVHDNTFLNLGYRYDIFFSRIDTIDNNS
                     SRGNHVANLGVGFTWGGAPSKP"
     gene            989..2107
                     /locus_tag="VMZ02_07585"
     CDS             989..2107
                     /locus_tag="VMZ02_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUR71831.1"
                     /translation="MTDSKRRRPVRYAVIGLGHIAQVAVLPAFAHARTNSQLAALVSD
                     DPQKLKILGRRYHVDQLFSYKEYGDCLRSGVVDAVYIALPNHLHAEYTIAAARTGIHV
                     LCEKPMAVTEKDCRAMIQAAERGGIKLMIAYRLHFEAANMTAVRIVRSGKLGDPRYFA
                     SSFSMQVRPDNIRTDREKGGGTLYDIGIYCINAARYLFGAEPTEVFAFSSARKDDDRF
                     KEIDETTGALLRFRDGRLAEFTSSFGAADVSSYRMVGTKGDVRLDQAYEYVYPIDVYV
                     TIKGKTRKQSFRKRDQFAPELEYFSDCILNDRTPEPSGMEGLADVRVIEALYRSAQRG
                     RPVKIEAVRRTQRPSMKQTRQRPPVDKPELVHAKSAAQ"
     gene            complement(2304..2648)
                     /locus_tag="VMZ02_07590"
     CDS             complement(2304..2648)
                     /locus_tag="VMZ02_07590"
                     /inference="COORDINATES: protein motif:HMM:NF021125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HUR71832.1"
                     /translation="MALVRCVTEMGMGVDVHGLDYTNAAKRAVFDAIHHSSLGFPRLV
                     GKTADDMHVEVTIGVPKPEAVDKRAVLESLPHGGGHVNVVQGGLQVLNEKGDDGWLIA
                     NAIIVVSIDDGK"
     gene            complement(2833..>3648)
                     /locus_tag="VMZ02_07595"
     CDS             complement(2833..>3648)
                     /locus_tag="VMZ02_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71833.1"
                     /translation="PDVATPQQARAKVDETVAAGADYIKIWVDSGFGTRAKLTAEFCA
                     AVLEQARKHNKLTFGHAYELIDARMLVERGLNVLAHNVRDREIDGDVIALLKQRNVSV
                     IPTLIRDEFLFAYGDAPAWIDEPFFQRFVPPERLALLKTKIRDEQAKNPQRALFKAGF
                     EMNKVNLKKLSDAGVRIALGTDSGGAADRFFVQGYAEHREMELMVQSGLTPMQVIQSF
                     SQGASEALGIVKDFGTLAKGKAADLLVLEKNPLENITNMRSMQTIYLGGKKFD"
BASE COUNT          793 a         1033 c          978 g          844 t
ORIGIN      
        1 gttcggcgag gcaagggcgt tcctggtgtc tttctagcct ttacttctcg tagagccgta
       61 acgaaaccgc tcgcctctat ttccctggct tcgtttttac tgacgggaca actcgtcaga
      121 catcgattcc cagttctgag atgatttcga catgggcgcc ataataacaa gtcgttaacc
      181 gcacgatacc tcgcagtccc gcgcgtttac gcaggagttc gtgctgtgat gaacgtcgtc
      241 ttatgtggaa tagcatttgc gttttatttc gaccaatcga ttgaagtgga ccggtttact
      301 caattgcgtg gcggtttggc atacgagtga gatgaataaa cattaataca cggaggtgta
      361 ctcatgcggc gattaggcgg tttgttttca agcacgttat tggtgggatt actgctcggg
      421 accggtactg cgagcgcatt acccggcgac ggcgcgagcg aagtccaagt atcggcgggt
      481 tttaaccaaa cgcaaggttc agacacggga agtttgaacg ttgacctcag ctatggctat
      541 tacttaacgc ccggctggca actcggattt cgccaggcca tcaactctac gttcgtcgac
      601 aatggccgcg atttttggct ggcgaccact actcccttcc tcaattataa cttccggctc
      661 accaacatca tcgttcctta cctcggcgca ttcatcggtg cagcctggaa tgatcgagac
      721 atcaccggaa ccgctggacc gcaagtcggc atcaaatttt tcgttcatga taacaccttt
      781 ttgaacctcg gctaccgcta cgacatcttt ttctcgcgga tcgacaccat cgacaataat
      841 tccagccggg gaaatcatgt ggcgaacctc ggtgtcggct tcacctgggg cggcgctccg
      901 agcaaaccgt aagctctgag caaaattcaa cgacggcggc gatcgaaatg gacttcgatc
      961 gccgccgttc cggaatgcgg ctggagaaat gaccgactct aaacggcgcc gcccggtgcg
     1021 ttatgccgtg atcggattgg gacatatcgc ccaagtcgcc gtgctccctg cgttcgcgca
     1081 tgcgcgaaca aattcccaac tggcagcttt ggtctccgat gatccgcaga agctaaaaat
     1141 tctcggtcgg cgctatcacg tcgatcaact tttttcttac aaggaatacg gtgattgctt
     1201 gcgcagcggc gtcgtcgatg ccgtctatat tgctttgccc aatcatctcc atgccgaata
     1261 caccattgca gcggcacgga cgggcattca cgttttgtgc gagaagccca tggctgtaac
     1321 ggaaaaagat tgccgggcca tgattcaggc cgcggaacgt ggcggaatta aactgatgat
     1381 cgcttaccgg cttcattttg aggccgcgaa tatgacggcc gtgcgaattg tccgctcggg
     1441 aaaactcggt gatccgcgtt acttcgcgtc gtcgttttct atgcaggtac gtccagacaa
     1501 tattcgcacc gacagagaaa aaggcggcgg tacgctctac gatatcggca tctattgtat
     1561 caatgctgca cgctatctct tcggcgcgga accgactgag gtttttgcat tcagcagcgc
     1621 ccgcaaagat gacgatcggt tcaaagaaat cgacgaaacg accggtgcct tgctgcgttt
     1681 tcgcgacggt cgattggcgg agtttacctc aagtttcggg gccgcggatg tcagcagtta
     1741 tcgtatggtc ggtaccaagg gtgatgtgcg cctcgaccag gcttacgaat acgtttaccc
     1801 catcgacgtc tatgtgacga tcaagggcaa aacccgcaaa caaagctttc gcaagcgtga
     1861 tcagtttgca cctgagttgg aatatttctc ggactgtata ctaaacgacc gcacgccaga
     1921 accgtcggga atggaaggac tcgcagatgt gcgcgtcatc gaagcactct atcgttcggc
     1981 acaacgtggg agaccagtaa agattgaagc ggtgcggcga actcaaagac cgtcgatgaa
     2041 gcaaaccaga cagcggccgc ccgtcgacaa gcccgaactt gtgcacgcaa aaagtgcggc
     2101 gcagtaacgc ttgtttttat gatggccggt agccgacata cagccgctaa tgccgcaatg
     2161 gcttaggcga cgcgaggcgg tgcggccggg gcatacatcg gccgtaaaca gtgatccgtt
     2221 agaagaggga tagcgacaaa acttcccgtc gcctaaatac gagagctttc ccgagatccg
     2281 gaggaatgtg ccagtggagc gaactattta ccgtcgtcga tgctgacgac gataatagcg
     2341 ttggcgatca accaaccgtc gtcgcccttt tcatttaaaa cctgcaaacc gccttggacc
     2401 acattgacgt ggccgccgcc atgcggcagt gactccaaga cggcccgctt atcgaccgcc
     2461 tcgggcttgg gcacacctat ggttacttcc acgtgcatgt cgtcggcggt cttgcccacc
     2521 aatcgcggga atcccaaact gctgtgatga atcgcatcga aaaccgcgcg cttggcggcg
     2581 ttggtgtagt ccaacccgtg cacatccaca cccattccca tttcagtaac gcatcgtacc
     2641 aaggccatag ctgttcctcc atcgtgagct ggcgtcgaac cgattcttta ccggcgctca
     2701 ttagacaacg ggaggaatcc tccgtcaagc agtctgcctg atttgacaac tattctcgtc
     2761 gtcaatcgct ccgcgtgtct cgctgcttcg ctgtgcttca ccaaccgcag cgtggccacg
     2821 actcgggcaa gcctaatcga acttcttccc gcccagataa atcgtctgca tcgaacgcat
     2881 gttggtgata ttttccaggg gatttttttc cagcactaat aaatcggcgg ccttcccctt
     2941 ggctagggtg ccaaaatcct tgacgatccc gagggcctct gaagcgcctt gggaaaacga
     3001 ctggatcacc tgcatcggcg tcagccccga ttgcaccatc agctccatct cgcggtgctc
     3061 ggcatagccc tgcacgaaaa agcggtcggc ggccccgcca ctgtcggttc ccaacgcaat
     3121 gcgcactccg gcatcggaaa gttttttaag attgactttg ttcatttcga aacccgcctt
     3181 gaacaaagcg cgttgggggt tcttcgcctg ctcatcacgg atcttggttt ttagcagcgc
     3241 cagccgctcc ggcggcacga atctctggaa gaacggttcg tcgatccagg ccggcgcgtc
     3301 accgtaggcg aataggaact cgtcgcgtat caatgtcgga atgacgctga cgttgcgttg
     3361 cttgagcagc gcaatcacgt cgccgtcaat ctcgcgatcg cggacgttgt gggccagtac
     3421 gtttaagccg cgctcgacga gcatcctggc gtcgatcagc tcataggcgt gtccgaatgt
     3481 gagcttgtta tgcttgcgcg cctgctcgag aacggccgcg cagaattccg cagtgagctt
     3541 ggcgcgcgtg ccaaagccgc tgtctaccca tatttttatg tagtcggcgc ccgccgcaac
     3601 cgtctcatcg accttggcgc gcgcttgttg cggcgtcgcc acgtcggg
//
LOCUS       DATLCH010000258         8426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2924845, whole genome shotgun sequence.
ACCESSION   DATLCH010000258 DATLCH010000000
VERSION     DATLCH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2924845"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VMZ02_07600"
     CDS             complement(<1..583)
                     /locus_tag="VMZ02_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71834.1"
                     /translation="MPWQNNGDYYSFKQDAITQHAPTTSGVYGLFNSRHLIAIGSAAN
                     VRDALLHHQRQTDFRFSRFEPSGFTFEICAPDRRDTRAQELVREYRPISTPENPIGIA
                     TLYRSWRAPNARAFQEVSIDGETASNKIVTIATKLRAAKQKMPLHLHAERFGVVGALC
                     GLIFVALGLIGLVPYLKNTFATVVRNPPAIAESR"
     gene            898..1920
                     /locus_tag="VMZ02_07605"
     CDS             898..1920
                     /locus_tag="VMZ02_07605"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71835.1"
                     /translation="MANYKVIDADGHVRESNAGMREFLEPRWQRRNLFPNDEWDRDLR
                     GKLGQKPEGPKDQLAAMDEDGIDVMVMYPTAGLHVSALHEKDYATAVTRAYNDWLYHF
                     CKTDLARLKFVALLAPQDPHAAAEELIRAVKERGAVAGVLPTYLPQMPDFGDRFYDPI
                     YAAAENLGIGLGFHTGTSADSLGGQRFRKFLSAHTIDHPAEQMMAMIATIVGGVFERF
                     PKLSIAYLESGIGWVPYMMDRLDEEVEKRGADEAPYLTQLPSEYVKSGRVFFGVECEE
                     KTIPDAMRWGLEDTILYSSDYPHWDGDWPHTVKGIRKRTDLTDVVKKKMLHDNVARFY
                     RLESAV"
     gene            2119..2427
                     /locus_tag="VMZ02_07610"
     CDS             2119..2427
                     /locus_tag="VMZ02_07610"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HUR71836.1"
                     /translation="MIVVSNRIQVAKGHEADFEKRFEGRARLVENMPGFIRLEILRPV
                     KSDYYVVLTHWQDEASFRAWADSTEFKEAHNKRPPAEIFAGPNVFEMHEVIQHAEKPR
                     "
     gene            2543..3532
                     /locus_tag="VMZ02_07615"
     CDS             2543..3532
                     /locus_tag="VMZ02_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71837.1"
                     /translation="MKTTVSWIATIVLLMSSAAGVHAAVPLNQLLITFGSLSERETVI
                     FVAKDYGIFNKYGLDVRAVHVRNGAVALSALANGEAQFYMGAATGSTLGAIANGMDAV
                     FITSLIHTLEGTFVVNPAIKVPADLKGKNIGVTSLGGGIWMRSMLAFEHWGLEPQRDK
                     ITLRVIGDESLLAQAMPNKVIDGAYFNYTFAGVLERQGFRVLADLAKLNIPYQNTAFL
                     AQRRFLNSAPDTVERLLRALSDTITFVLEPKNKSLVLQSMIKGLRLARSEDAVGGYER
                     LGSMYQRRIFPNVDGIRSTIRFLGATNEKIRALKAEDLVDERFVKKLEQEGRF"
     gene            4656..5171
                     /locus_tag="VMZ02_07620"
     CDS             4656..5171
                     /locus_tag="VMZ02_07620"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HUR71838.1"
                     /translation="MNAITERTLRRDQRGQSIVEISLITPLLLIALYIPADFGVSFFM
                     GNLIQTAAREGARIGSGLQKSGSAPSLLFSSGEATTVKAEVISRLPAYLTNKSVTVKF
                     YTGTACMEFVEVTAQGQYKFFMYQLMRLFGANAPNSVTISRTTQMHYKYQPYQNNDYC
                     TGVTTYGPYSA"
     gene            5188..6213
                     /locus_tag="VMZ02_07625"
     CDS             5188..6213
                     /locus_tag="VMZ02_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HUR71839.1"
                     /translation="MQPVKNQQGYVVVFVTLMIVLLLIMVGMGLDTGHLAYVRSQGQP
                     AVDSAALAAASAIPSGNWSTVTDRAAKFNPGGTNPGTGNNYLDSPSNKINQKNVTLVK
                     YDNTSGEFTTTGVNINNANGARVALENNNPYGGAAAKSIKSPLFLAPLLNMFGQSTKN
                     TTDVSVSAVAVNQALPGLPIVAIESLCSKTGDVELDFQSGGKSTAGWETYQIINASTD
                     EVRGLFEKLPYCAGQPAIDTGYCGNVANGVNATVFNQNLKPMFKADPSRCYLIPVIPS
                     GGNLNQCQTISDWAKFCPAQGNNTADAFPSQGVLKGRVTCGQSPWTSRDSQCYAQRLV
                     RDKTSGM"
     gene            complement(6376..>8426)
                     /locus_tag="VMZ02_07630"
     CDS             complement(6376..>8426)
                     /locus_tag="VMZ02_07630"
                     /inference="COORDINATES: protein motif:HMM:NF028231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HUR71840.1"
                     /translation="YLPYKPKRRSKAQIAREAGLEPLADALLGDSNLDPQEEAAKYVN
                     DAAENGVPDIKAALEGARDILSERFAETADLLAKLRARMWEQGVVTSKVMKDKEMAEE
                     EKFRDYYTYCENIRSIPSHRALALFRGQSLGVLAIDLTLDEKHEILVPHPCVAMIAGH
                     FGIADRGRRADKWLADVCNWTWRVKAHPHISTELLMQLREAAEAEAIKIFGRNLHELL
                     LAAPAGPKAVLGLDPGIRTGCKVAVVDATGKLLETATIYPHQPRNEWEGSLATLARLT
                     VQHNVELISIGNGTASRETDKLAAEVIKLIAAKMPERKINKIVVSEAGASVYSASAFA
                     AAEFPELDVSLRGAVSIARRLQDPLAELVKIDPKAIGVGQYQHDVNQRALARNLDATV
                     EDCVNAVGVDLNTASVPLLTRVSGLNSLLAKNIVEFRNGNGPFRNRKSLTKVPRLGEK
                     TFEQAAGFLRINDGDNPLDRSAVHPEAYPVVERILARVGKGIGEVMGRSAVLKELQPE
                     QFTDEKFGVPTVRDILAELEKPGRDPRPEFKTANFQEGVETMADLQAGMILEGVVTNV
                     AAFGAFVDIGVHQDGLVHVSALANKFVKDPHEVVRPGQIVKVRVMEVDLKRQRIALTM
                     RLTDAPSDTLRSNQARPEANTAPSQRDKRPGLSSSQAGRQAQPTGLFAIALEKAKQKK
                     "
BASE COUNT         1906 a         2211 c         2337 g         1972 t
ORIGIN      
        1 gtcgcgactc ggcgattgcc ggcggatttc ggacaacggt tgcgaaggta tttttcagat
       61 agggcacgag cccgatcaaa cccaaagcga cgaaaatcag tccgcacaaa gcgccgacca
      121 cgccaaatct ctccgcgtgc aagtgcaacg gcattttttg tttcgctgcg cgcagcttgg
      181 tcgcaatcgt cacaatttta ttgcttgctg tctctccgtc gattgacact tcttggaatg
      241 cacgcgcgtt gggagcacgc cacgagcgat ataatgtggc gatgccgatg gggttttccg
      301 gtgtgctgat cggacggtat tctctaacca attcctgggc tctggtgtcg cgccgatccg
      361 gggcgcagat ttcaaatgtg aaaccactcg gttcgaagcg gctgaagcga aaatctgtct
      421 gccgctgatg atgcagcaga gcatcgcgaa cattcgccgc gctgccaatc gcgatcagat
      481 gacgagaatt aaatagaccg tacacacccg atgtggtcgg tgcatgctga gtaatcgcgt
      541 cttgtttgaa gctgtagtag tcgccgttat tctgccaggg catagagtct ccattcttct
      601 acattgcgga cccaacgata aaaatgttcc gctcggcgct tgctcccacg cgccggaaca
      661 gcccccgtcc aaaaattgcc gcaatgtaag tattcgaagt aataaagcag ttattgcgat
      721 cccaagcaag aaaaatgtcc aatttaggac cgtgtgcaat ggcaagcgtc gtaaaaattg
      781 ccgattgatc gcgcgcggat cagattgaca tcctcttttc cgcggtgtta tggctcgacc
      841 gaactgtttg gccgctgaaa atcgttgcag ttagcggcgc gaaaggagtt catcgatatg
      901 gcgaactata aagtgatcga tgccgatggc catgtgcgtg aatcgaacgc aggcatgcgc
      961 gagtttttgg aaccacgttg gcagcggcgc aatctttttc ctaacgacga gtgggaccgt
     1021 gatctgcgcg gtaagctcgg tcagaagccg gaggggccga aggatcagct cgcggcgatg
     1081 gacgaagatg gcattgacgt catggtgatg tatccgaccg cggggctgca tgtctcggca
     1141 ctgcacgaaa aagattacgc caccgctgtc acgagggctt ataatgactg gctctatcat
     1201 ttttgcaaaa ccgacttggc gcgactgaag ttcgtcgcgc tgttggcccc ccaagaccca
     1261 catgcggctg cggaagaatt gatccgcgcc gtgaaggagc gtggcgctgt ggctggcgtg
     1321 ttaccgacct acttacccca aatgccggat ttcggtgaca gattctacga tccgatttat
     1381 gctgccgctg agaatcttgg catcggtctc ggatttcaca caggcacctc cgctgattca
     1441 ttgggcggtc agagattccg caaatttctt tcggctcaca ccatcgatca tccagccgag
     1501 cagatgatgg cgatgatcgc aacgattgtc ggcggcgtgt tcgagcgttt ccccaagctc
     1561 agcattgctt atctcgagtc gggcatcggt tgggtgcctt atatgatgga ccgcctcgac
     1621 gaagaagtgg aaaaacgcgg cgccgacgaa gcgccttacc tgacccagct gccgagcgaa
     1681 tacgtcaaga gcggccgggt ctttttcggc gtagaatgtg aggagaagac cattcccgat
     1741 gccatgcgct ggggcttgga agatacgatt ctctattcgt ccgattatcc gcactgggac
     1801 ggcgattggc cgcacaccgt gaaaggcatt cgcaaacgca ccgatctgac cgatgtcgtg
     1861 aaaaagaaga tgctgcacga caacgtcgcg cgcttttatc gcttggagtc ggcagtatga
     1921 tacgtctgca agattcacca acagctaatg ctgaaaacaa ttctgccggc cagctgctaa
     1981 gcgactcttt cagaagtatt ttgcccctcg gaggaggtaa aaggatttac aagcaggaag
     2041 gtaatcaggt tgtagtatcg ccgcagagtt ggacaaattg acaactccaa accgtgcttt
     2101 gaacgaaagg aaattgtttt gattgttgtc tccaacagaa ttcaagtggc caagggtcat
     2161 gaagctgatt ttgaaaagcg cttcgaaggc cgggctagac tcgtcgaaaa catgccgggc
     2221 tttatccggc tggaaattct tcggccggta aaaagcgact actacgtcgt tctaacccat
     2281 tggcaagacg aagcttcgtt tcgcgcctgg gccgacagca cggaattcaa agaagcccac
     2341 aacaagcggc caccggcaga gatattcgcg gggcccaatg ttttcgagat gcacgaagtg
     2401 atccagcatg cggagaagcc gcgataatcg cgttcattcg gaatatttct cgcatgtact
     2461 cgtcttttcg aacatagcgt ctttgcgcct tcgcgcgaaa atatcctaag actttcctga
     2521 catcatacta ggagtgtgct gcatgaaaac gaccgtcagt tggattgcta caattgtact
     2581 actgatgtcg agtgctgccg gcgtccatgc tgcggtgccg ctcaaccagc tgctgatcac
     2641 gttcggcagc ttgagcgagc gcgaaacggt aatattcgtt gccaaagact atggcatttt
     2701 caacaagtat ggcttggatg ttcgcgccgt tcacgtccgc aacggggcgg tggccctgtc
     2761 ggcgctggcc aacggcgaag cgcagtttta catgggcgcg gcgaccggct caaccctcgg
     2821 cgcgattgcg aacggcatgg atgcggtgtt catcaccagt ttgattcaca ccctggaagg
     2881 caccttcgtt gtcaacccgg cgatcaaggt gccggccgat ttgaaaggca agaacatcgg
     2941 cgtcacgagc cttggcggcg gcatttggat gcgctcgatg ctcgccttcg aacactgggg
     3001 tctcgagcct cagcgcgaca agattacttt gcgcgtgatc ggtgacgagt cgttgctcgc
     3061 ccaggcgatg cccaacaaag tgattgacgg cgcctatttc aattacacat tcgccggtgt
     3121 tctcgaacgc cagggctttc gcgtgctcgc cgatctcgcc aaactaaaca tcccatatca
     3181 gaacaccgcg ttcttggctc agcgaagatt tttgaacagc gctcccgata ctgtcgaacg
     3241 attgctccgc gcgctgtccg acaccatcac ttttgtttta gagccgaaga ataaatcgtt
     3301 agtcctgcaa agcatgatca aaggcctgcg actggcgcgt tccgaagatg ccgtcggtgg
     3361 ttatgaacgc cttggcagca tgtaccagcg ccgcatattt cccaacgttg acggcattcg
     3421 ctcgacgatc cgctttctcg gcgccaccaa tgagaagatt cgcgcattaa aagccgagga
     3481 cttggttgat gaacgatttg ttaagaagct ggagcaggag gggcggtttt aggttgtgca
     3541 tgacagcaaa gcaataccga tcttaggacc gggaagttta actcacctcg aaacggtcac
     3601 ttgccgatca aaacatcgat tgaaagaaaa tagcccagca gatttctttc gtgctaggcg
     3661 attctgttgg gagaaagttt acgagaaata tcgacgcgat aaggcacgtt cagtagcggc
     3721 gctgctattc ctctgtcttg ggcatgacag ctttatcttt tcccgctgtt tcggttaatg
     3781 cacatctcgg tttgcgacaa tccacgaatg tttctccctt tgacgctcgt cctatgacga
     3841 tccgattgat ttcgatccaa ctcaggattc gctgaatcaa attctcaaga ctgtgcttcg
     3901 atccacctag gtagatagtg gtcaattttg tttcgcgcgg cgttgaatgc acattagcgg
     3961 cttacgccga acgacaaaag tgacaccgga atgacgatat cgtcactggg ttgtcaatac
     4021 ccagtaatgg ccgcgatctt gccatggtgc aggtgcacgc actgcgaccg ctttaggtca
     4081 aacggctaaa gaaaggagtt cgactgatgt tcgtccatgc gtattccccg cgccttgccg
     4141 gaaatgcttg caatcgttta tctcagtcgt caggccaggc tcttgtcgaa ttcacgttca
     4201 catttctaat attgctgatt gtcgcatgga tacctgctga ctttggcctt gcgttttata
     4261 ccggccagat tgctcagaat gctgcgcgtg aaggggccag aattgcggct actgaacgtt
     4321 cgctcgtaag cggaacaacc agctgtacca tgccttcgtg tacgggaaat atttttaccg
     4381 aaacggcagc aaggctttcg tcggcgctgc ttccggccgc taccatcaca ctgcagttgg
     4441 atcctgacac tggtaccaat tgcaaccgca tggtcacggt aaccgtttcc ggtacttaca
     4501 aattcgtctt ctaccaattt ctgcgctgga tggggatctc aggaaaccta aacaataagc
     4561 ctataacccg gcaaactcgc atgagatggg agccgcaaac gagttgctag ccgatggaat
     4621 tgaagagaac gaccgatgaa tgggaggaga agtttatgaa cgccataacc gagcgaacgc
     4681 tgcggagaga tcaacgcggt caaagtattg tggagatttc actcataact ccgcttttgc
     4741 tgattgcgtt atacattccc gccgatttcg gcgtttcatt ttttatgggc aaccttattc
     4801 agacggcggc tcgtgaggga gctcggatcg gcagtggact gcaaaagagt ggcagcgccc
     4861 ccagtctact tttcagctcg ggggaagcga ctacggtgaa agcggaagtg attagccgtt
     4921 tgcctgccta tctgacaaat aagtctgtca cggtaaaatt ttatacagga acggcgtgta
     4981 tggaatttgt cgaggtgacg gcgcagggcc agtacaaatt tttcatgtat cagttaatga
     5041 gattgtttgg cgccaacgcg ccgaactccg tgacgatctc gcggacgacg cagatgcact
     5101 acaaatatca gccgtatcag aacaatgact actgtacggg tgtgactact tacggaccgt
     5161 attcagctta ggtcgatgga ggctaatatg cagccggtca aaaatcagca aggctatgtc
     5221 gtcgtctttg tgacgttgat gattgttttg ctcttgatca tggtcggcat ggggttagat
     5281 accggacacc tggcctatgt tcgatctcaa ggacagccag ccgttgattc agcggcattg
     5341 gctgcggcct ctgcaattcc aagcgggaac tggtcgacgg ttacggatcg tgccgcaaaa
     5401 tttaatcccg gcggcaccaa tcccggcact ggaaacaatt atctcgacag tccgagcaat
     5461 aagatcaatc aaaagaacgt cacactggtg aaatacgaca acacgtcggg ggaattcacc
     5521 accactggtg tcaatattaa caacgccaac ggcgctcgag tagcattaga aaataacaat
     5581 ccgtacggcg gcgctgccgc aaaatcaata aaatcaccgc tgtttttggc gccgctgctc
     5641 aatatgtttg gtcaatccac gaaaaacact accgatgtga gtgtcagcgc ggttgccgtg
     5701 aaccaggcgc ttcccggtct accgattgtc gcgatcgaat ctctctgtag caaaaccggt
     5761 gatgtggagc tggatttcca atcgggaggc aagagcacgg ccggctggga aacttaccag
     5821 ataattaacg ccagtacgga tgaggttcgc ggactttttg agaagctgcc ttattgtgcg
     5881 ggccaaccgg caatcgatac tggatattgt ggaaatgtgg caaacggcgt caacgcaacc
     5941 gtgttcaatc agaatctaaa accgatgttc aaggcagatc catcgcgttg ctatttgatt
     6001 ccggtgatac cgagcggcgg taacctgaac cagtgccaaa ccatctcgga ttgggcgaaa
     6061 ttttgccccg ctcaaggaaa caataccgcc gacgcattcc ctagccaagg cgtgcttaaa
     6121 ggacgtgtca cttgcggcca aagtccgtgg acgtcgaggg acagtcaatg ttacgcccag
     6181 cgcctggtcc gcgataaaac atcggggatg tgaagtacac tacgcgaact tacgtgatgg
     6241 ctgtcccggc tcggtaacgg tttgtcgcaa ctgcacttcg ctgtgactac tagaaggaca
     6301 atctctcaca gtcgtctatg agctgatcaa agtacgtctt cccgcctcag cgacgcagca
     6361 attaacatag ggcgatcatt tcttctgctt ggctttttcc aatgcaatag cgaacagccc
     6421 tgtcggctgc gcttggcggc ctgcttggct gctggatagc cctggccgtt tgtctcgctg
     6481 gctgggtgcc gtattcgctt ccggacgcgc ttggttcgaa cgtagcgtat cgctgggggc
     6541 gtcggtgagt cgcatggtca aggcaatgcg ctggcgtttc aggtcgactt ccatgacgcg
     6601 caccttgaca atttggcctg gcctgacaac ctcgtgagga tctttaacga atttgttcgc
     6661 cagcgcggag acatggacca agccgtcctg gtgcacgccg atgtcgacga aagcgccgaa
     6721 ggcggcgacg ttggtaacga cgccttcgag aatcatgccg gcttgtaaat cggccatcgt
     6781 ctcgacgcct tcctgaaagt tcgcggtttt gaattccgga cgcggatcgc ggccgggttt
     6841 ttccagttct gccaaaatat ctctgactgt cggcacgccg aacttttcgt cggtaaactg
     6901 ttcaggttgg agttctttca atactgcgct tcggcccatc acttcgccaa tgcctttgcc
     6961 gacgcgcgcc aaaatccgct caacgacggg ataggcttcc ggatgaaccg cggaacgatc
     7021 gagcggattg tcaccatcat tgatgcgcag aaagccggcg gcttgctcga aggtcttctc
     7081 gcccagacgc ggcactttgg tgagcgactt gcgattgcgg aacgggccat tgccattgcg
     7141 gaactcgacg atattcttgg cgagcagact gttgagccct gacacgcggg tgagcagcgg
     7201 caccgatgcg gtattgagat cgacaccgac ggcgttgacg caatcctcga cggtagcgtc
     7261 gaggttgcgc gccagcgcac gctgattgac gtcgtgttgg tactggccaa cgccgatcgc
     7321 tttaggatcg atcttgacca actcggcgag cggatcttgc aagcggcggg cgatcgacac
     7381 cgcgccgcgc aggctgacgt cgagttcggg aaattccgcg gcggcgaatg cagaggctga
     7441 atagaccgag gcgccagctt cgctgacgac gattttattg attttgcgtt ccggcatttt
     7501 cgcggcgatt agcttgatca cttcggcggc caacttgtcg gtttcgcggc tagcggtgcc
     7561 gttaccgatg gaaatcaatt cgacgttatg ctgcaccgtc aggcgcgcca aggtcgccag
     7621 tgaaccctcc cactcattgc gcggctggtg cgggtaaatg gttgccgttt ccaggagctt
     7681 gcccgtagca tcgacgacgg cgactttgca gcccgtgcga atgccgggat cgaggccgag
     7741 caccgctttc ggtcccgccg gagcggcgag caggagttcg tgcaagttgc ggccaaatat
     7801 tttgatcgct tcggcttcgg cggcttcgcg caactgcatc aacagttcgg tgctgatgtg
     7861 cgggtgcgct ttgacgcgcc aagtccaatt gcatacgtca gcgagccact tgtcggcgcg
     7921 gcggccgcga tctgcaatgc caaaatgacc ggcgatcatg gccacgcagg ggtgcggcac
     7981 cagaatttcg tgtttttcgt cgagagtgag atcgatggct aaaacaccca agctttgccc
     8041 tcgaaatagc gctaacgccc ggtgcgatgg gatggagcga atgttttcgc agtaggtgta
     8101 ataatcgcga aatttttcct cttcggccat ttccttgtct ttcatcacct ttgaagtgac
     8161 gacgccttgt tcccacatcc gcgcgcgcaa tttggcgagc aaatcggcgg tctcagcaaa
     8221 acgctccgac aaaatatcgc gggcgccttc gagggcggct ttaatgtcgg gcacgccgtt
     8281 ctcggccgca tcgttgacat acttcgccgc ttcttcttgc gggtcgaggt tggaatcgcc
     8341 gagtagcgcg tcggccaacg gctcgagacc agcttcgcgg gcgatttgag ccttgctgcg
     8401 gcgcttgggt ttgtagggca aataga
//
LOCUS       DATLCH010000259         4812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8544605, whole genome shotgun sequence.
ACCESSION   DATLCH010000259 DATLCH010000000
VERSION     DATLCH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8544605"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VMZ02_07635"
     CDS             complement(<1..522)
                     /locus_tag="VMZ02_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71841.1"
                     /translation="MAERGVDQKEEKKSYGSVFVLGAAILAALSLWAFLDDNITRRPW
                     KNFQSKFYRLDYNKAKAAYDEEDKKLQADDKYKELSQKLAAFEASLASGDLGKRLKAL
                     ESEEARTTVKFQEVDQKVKDVKSELEEAWYEHDHAVQQKRNPQPYLDVIAALDKKKAA
                     LDQQLTPLRDRRNQ"
     gene            complement(533..700)
                     /locus_tag="VMZ02_07640"
     CDS             complement(533..700)
                     /locus_tag="VMZ02_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71842.1"
                     /translation="MFYVAKILQALGLADIGYALFVGIMGNSSMAEEIVLTLIGLGVF
                     SLGRALERKVA"
     gene            complement(701..1468)
                     /locus_tag="VMZ02_07645"
     CDS             complement(701..1468)
                     /locus_tag="VMZ02_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71843.1"
                     /translation="MADAAPQRKLPVDDKIHTWPHLVRMEFLVALFVMIGLTIWSLTI
                     DAPLEEPANPTRTPNPSKAPWYFLGLQEMLVYFDPWHAGVVLPTLIIVGLMVIPFIDT
                     NPRGNGYYCFKDRKWEILTFIFGFHVLWVVMIIIGTFFRGPGWNLFWPWQHWDPHKVV
                     ALTNVDLPYLLGIRSYGLAAAFGLLAIGAYFVGGMLLFYWFVIKVKGKEFLERWGMVR
                     FTITGVILVLMMSLPAKMFLRLAFNVKYVLVTPWINI"
     gene            complement(1493..2263)
                     /locus_tag="VMZ02_07650"
     CDS             complement(1493..2263)
                     /locus_tag="VMZ02_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HUR71844.1"
                     /translation="MSLFEKIKSDIVESQVWKAIFRHGYDDTPRNRVMMVTSNVFLHL
                     HPAKVRRHAVRMRFTWCMGGLTFLMFLITAITGIYLMFYYRPVAEYAYADMKYLEFDM
                     PFGMLMRNMHRWAAHAMIVFVWLHMFRVFLTGSYKPPREFNWIVGVLLLVLTLLLSFT
                     GYLLPWDQLAIWAVTVGSNMARATPLLGYEGPFAEFVGANPIYDARAFLFGGGEIGPH
                     TLLRFYILHCIFIPLVASILMAVHFWRIRRDGFSGPSL"
     gene            complement(2275..2793)
                     /locus_tag="VMZ02_07655"
     CDS             complement(2275..2793)
                     /locus_tag="VMZ02_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR71845.1"
                     /translation="MAEAEKKKSGIWSRRDFFTRLGWGGFGVFGGLSLLGFLRSAFPR
                     VLFQPPASFKAGLPSDYAVGEVSEKYKQDQRVWIVREETGVYAIFAKCTHLGCTPRWL
                     AAENKFKCPCHGSGFYKSGLNFEGPAPRPLDRFKLSVGDDGQLVVDKNKIFRGTAGLD
                     PDEQYPDSVLKA"
     gene            complement(2796..3146)
                     /locus_tag="VMZ02_07660"
     CDS             complement(2796..3146)
                     /locus_tag="VMZ02_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71846.1"
                     /translation="MTSDPPNLAPEAREQEIERLAQELGRLIQGADPERRHDLKELAI
                     ALMREEFIHKDEAPPGVGTTPAPFNPLGTGVLLFFLGGALSFIFGPVGLLLMLSGVGF
                     VIWGGLISWLRKAK"
     gene            complement(3214..3696)
                     /gene="ispF"
                     /locus_tag="VMZ02_07665"
     CDS             complement(3214..3696)
                     /gene="ispF"
                     /locus_tag="VMZ02_07665"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HUR71847.1"
                     /translation="MAKQFRIGQGFDVHRFRRGRKLILGGVELPSPTGLLGHSDADVV
                     LHSVINALLGAMGEGDIGTHFPDTDSRYKGISSAKLLKVVLAIMRRKRFKIVNIDVTL
                     IAQRPKLSPHYSAMRKSVAGLLKLAERDVSIKASTTEKLGWIGKGQGMAGSAVVLLTR
                     "
     gene            3879..4616
                     /locus_tag="VMZ02_07670"
     CDS             3879..4616
                     /locus_tag="VMZ02_07670"
                     /inference="COORDINATES: protein motif:HMM:NF023984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HUR71848.1"
                     /translation="MLIIICLLIFIALPLYTVLSGQAPQGRDFRTADRSSTGLAPPAE
                     STREPVVQVYFARALNWRGIFGVHTWIATKPANATEYTVHQVIGWRIYRDLPAVVSGP
                     GIPDGRWFGNEPTLITDLRGEIAATAIAKIAEAIAKYPYANEYTLWPGPNSNTFIAYI
                     GRAVPELRMDLPSTAIGKDYPINGSLLDQTPSGTGYQLSMLGLVGLTIAREEGFELNL
                     LGLTFGVDFYKPAIKLPFIGRLGFGQN"
BASE COUNT         1183 a         1378 c         1191 g         1060 t
ORIGIN      
        1 ctggttgcgc ctatcgcgca gaggagttag ctgctgatcc aaagccgcct tttttttgtc
       61 cagagcagcg atgacatcga gataaggctg tggattgcgc ttctgctgca ccgcgtgatc
      121 atgctcgtac caggcttcct caagctcgct ctttacatcc ttaacttttt gatcgacttc
      181 ctgaaacttt acggtcgtgc gcgcttcttc gctttccagc gctttgagcc ttttgccgag
      241 gtcgccgctt gccaagctgg cttcaaaggc cgcgagcttc tgagataatt ctttatactt
      301 gtcgtccgct tgaagtttct tgtcctcttc gtcgtaagcg gccttggcct tgttgtagtc
      361 aagccggtag aacttcgact gaaaattttt ccaaggtcgg cgcgtgatgt tgtcgtcaag
      421 gaaagcccac agggacaacg ctgccagtat ggccgcgccg aggacgaaaa cactgccgta
      481 ggacttcttc tcttcttttt ggtcgactcc ccgttccgcc atcccgactc ccttacgcta
      541 ccttgcgctc cagcgcacgg cccagtgaaa ataccccgag accaatgagc gtcaagacaa
      601 tttcttcagc catggaagaa ttacccatga tgccgacaaa caacgcatag ccgatgtccg
      661 caagccccaa ggcttgaaga attttcgcca cgtaaaacat ctagatattg atccacggcg
      721 taaccaggac gtatttgacg ttgaacgcca gacgcaaaaa catcttggcc ggcaacgaca
      781 tcatcagcac aagaatcacg ccggtgatgg taaatcgcac catgccccag cgctcaagaa
      841 actctttgcc tttgaccttg atgacgaacc agtagaacag caacatgcca ccgacgaaat
      901 acgcaccaat ggcaagcaat ccaaacgccg cggccaaacc gtaactgcga ataccgagca
      961 aatagggcaa gtccacattg gtcaatgcca cgaccttatg cggatcccag tgctgccatg
     1021 gccaaaaaag attccaaccc ggaccgcgga aaaaggtccc gatgataatc atgactaccc
     1081 acagaacgtg gaaaccgaag atgaaagtca aaatctccca cttgcggtct ttaaagcagt
     1141 aatagccgtt gccgcgcgga ttggtgtcta taaacggaat caccatcaga ccgacgatga
     1201 tcaaagttgg taaaacgacg ccggcatgcc atgggtcgaa gtaaaccagc atctcttgca
     1261 agccgagaaa gtaccacggc gctttcgatg gattgggcgt tcgagtcgga ttggcaggct
     1321 cttcgagagg cgcatcgatg gtcaacgacc agattgttag tccgatcatg acaaacaacg
     1381 cgaccagaaa ctccatgcgc accaaatgtg gccaggtgtg aattttatcg tcgaccggaa
     1441 gctttcgttg tggagcggcg tctgccatag atcagcctcg ttttcggatc gcctataaag
     1501 atggaccgga aaacccgtcg cgccggatac gccagaaatg cacggccatc aaaatgctcg
     1561 cgaccagcgg aataaaaatg caatgcagaa tgtaaaagcg caaaagcgtg tgcggtccga
     1621 tctcgccgcc gccgaacaga aacgcgcgcg catcgtaaat cgggttggcg ccgacaaatt
     1681 cggcaaacgg gccttcataa cccaagagcg gtgtcgcccg cgccatgttg gaaccgacgg
     1741 ttaccgccca gatcgccaac tgatcccaag gcagaaggta tccggtaaaa ctcagtaaca
     1801 acgttagaac gagcagcaaa acgccgacga tccaattgaa ctcgcgcggc ggtttgtagg
     1861 aaccggtgag aaatacccgg aacatgtgca accaaacaaa gacgatcatg gcgtgcgccg
     1921 cccagcggtg catgttgcgc atgagcatgc cgaaaggcat atcgaactcg agatatttca
     1981 tatcggcata agcgtattcc gccactggcc gataatagaa catcaggtaa atgccggtga
     2041 tcgcggtgat aagaaacatc aaaaaagtga gaccgcccat gcaccaggta aagcgcatgc
     2101 gcacagcatg gcggcgcact ttggccggat gaagatggag aaagacattg gacgtcacca
     2161 tcatgacgcg gttacgcggc gtgtcatcgt aaccatgacg aaagatcgcc ttccaaactt
     2221 ggctttcaac aatatcgctt ttaatttttt caaacaggct cattattttt tccgttaggc
     2281 tttgagaacg ctatccggat actgctcgtc tggatcgagg cctgcagtac cgcgaaagat
     2341 tttgttcttg tcgacgacaa gctgcccgtc atcaccgaca ctgagtttga agcgatcgag
     2401 cggacgcggc gccggtccct cgaaattcaa gccggacttg tagaatccac tgccgtggca
     2461 tggacacttg aatttgttct cggcggcgag ccaacgcggg gtgcagccca agtgtgtgca
     2521 tttagcaaag attgcgtata ccccggtttc ttcgcggaca atccaaacgc gctggtcttg
     2581 tttatacttt tcactgactt cgccaacggc gtagtccgat ggcaagcccg ctttgaagct
     2641 cgccggcggt tgaaacaaca cgcgcggaaa agctgaacgg agaaatccca ggaggctcag
     2701 cccgccaaag acgccgaatc cgccccagcc taagcgtgtg aaaaaatccc gccgcgacca
     2761 gataccgctt tttttctttt cagcttctgc cataactatt tcgctttcct aagccagctg
     2821 atcaatccac cccagattac aaagccgacg ccactcaaca tcagaagtag cccaaccgga
     2881 ccgaatatga acgacaatgc gccgcccaag aaaaacaaca aaaccccggt tcccaatgga
     2941 ttaaaaggag ccggcgtcgt tcctaccccc ggcggcgctt catccttgtg gatgaactcc
     3001 tcgcgcatga gcgcgatcgc cagttctttg agatcgtggc gccgctcagg atcggcgccc
     3061 tgaatcagac ggccaagctc ttgagccaac ctttcgatct cttgttcgcg cgcttccggc
     3121 gctagatttg gaggatcgga tgtcaccaaa gattagaatt gttgattatt atagaaaata
     3181 ttctttgtca acaatttggc ccttcttgag acgttaacga gtgagcaaaa ctaccgcgga
     3241 accagccata ccttgtcctt tgccaatcca tccgagcttc tcggtagtgc tggctttgat
     3301 gctcacgtcg cgctcggcca acttgagcag gccggcaacg cttttacgca tcgcggaata
     3361 atgcggcgac aacttcggtc gctgcgcgat caacgtgaca tcgatattga cgatcttgaa
     3421 acgcttgcgc cgcatgatcg ccagcaccac cttaaggagc ttggcactgg aaatcccctt
     3481 gtaacgcgaa tcagtgtcag gaaaatgcgt gccgatatcg ccttcgccca tggctcccag
     3541 taaagcattg atcaccgaat gtaacaccac atcggcatcg gaatgtccca gcaagccagt
     3601 cggcgaggga agctcgacgc cgccgagaat caatttacgg ccgcggcgaa aacggtgcac
     3661 gtcgaaacct tgaccgatac gaaactgttt tgccatcgct gttgagtcgc agcaacatca
     3721 tgtcgtgaac cgtcgcgaca ttcaactgga agtcgataat cgacacgaac ggcgccaata
     3781 aagttgggat gaaacgcgcg ttatgatgtt caggttggct tcatgttggc tattcgctat
     3841 aatccgcgat aaaaaatctt ggacccatgc catcgcggat gctaatcatt atctgtttgc
     3901 tgatctttat cgccctgccg ctttataccg tgttatccgg tcaggcgccc cagggccgcg
     3961 actttcgcac ggcggaccgt tcgagcacgg gtctcgcgcc acccgctgaa agcacccgcg
     4021 agccggttgt ccaggtttat ttcgcccgcg cgctcaattg gcgcggcatt tttggtgtcc
     4081 atacctggat cgctaccaaa ccggcaaacg cgacggaata taccgtgcat caagttattg
     4141 gctggcgcat ttaccgcgat ctcccggcgg tggtatccgg cccaggcatc cccgatggcc
     4201 gttggtttgg caacgagccg acgttgatta ctgatctgcg cggtgagata gccgctacgg
     4261 cgatcgcaaa aattgccgaa gctatcgcca aatatccata cgccaacgaa tacacgcttt
     4321 ggcccggccc taacagcaat acctttatcg cttacatcgg ccgtgcggtg cccgaactgc
     4381 gcatggattt gccctccacg gctatcggta aggactaccc aatcaacggt tcactgctgg
     4441 accagacccc gagcggtacg ggttatcagc tttctatgct cggactcgtc ggcctgacca
     4501 tcgcccgcga ggaaggcttc gagcttaatc ttctcggcct cacgttcggc gtcgattttt
     4561 acaagccggc gatcaagctt ccgttcatcg gacggctcgg cttcggccaa aattgatcac
     4621 attgtgagct ttgaacgacc ttaaatagtt gcccaatcgc ttgactaccc gcaagcaaac
     4681 cctcccgacc aacttgttgc gccgttggtt tcaaagcagc gtgtttttca tcgccatccg
     4741 tcctagcgcg ccttagccat gagaaactct tcaccgatct tcctcccttc ggtcgcagac
     4801 atactcttct ta
//
LOCUS       DATLCH010000260         8327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7794427, whole genome shotgun sequence.
ACCESSION   DATLCH010000260 DATLCH010000000
VERSION     DATLCH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7794427"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VMZ02_07675"
     CDS             complement(<1..726)
                     /locus_tag="VMZ02_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HUR71849.1"
                     /translation="MKHPAPLIGITSDISVAGRGENRESLYFLAQRYIKAVADSGAVP
                     VILPPMPSSGAIVALLARIDGLILSGGNFDIHPSYYGEIPIPALGAIKAERSAFELEI
                     TAVALKQDLPVLGVCGGAQAINVVQGGSLYQDIGAQVPNAQEHQQSKKAIKRGHRITL
                     QPHTRLHAIIRRTHTEVNTTHHQAVKTLGRGLVINAIASDGIIEGIESTGHTFVIGVQ
                     WHPEVLAPRQVHQRSIFAALIASA"
     gene            complement(732..2798)
                     /locus_tag="VMZ02_07680"
     CDS             complement(732..2798)
                     /locus_tag="VMZ02_07680"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HUR71850.1"
                     /translation="MNRDVRLFTLKTLMKQTIWYCAALILLCLGWARVDAQNDSGRSA
                     FRKAYALFNQGNTTEAKELFHKTLEANYRLSDYSLHYLALIAFKERNWEQSRQYLLQL
                     KQRYPQSVWQFRATLQRAKIDQAEKKLAQANEILRQLRADKGTPREIVDEASYLLAQN
                     FEAQGDVQRAYYGYQEVRDNSPTSSWAGLARKDQTRLRDKYPEQFALSTIAALGDEAD
                     RLARERQLNDAAELYKRILDSVTDNDQRLRYLTKLAAVRGRNEAIAVLEQIARNFPDS
                     SEAPKALYQIGQTLWNRHENAQALEYFNQLVQRYSSSAYADRATFAAADIHEYFGRNS
                     EAIQLYTTVQKQFPNSTVRDDAVWRLAWLHYRSGDWQAALTTFKALAAQSRSSTFDTA
                     AVYWQARSTEKLGDVDTAKQIYRQIFNSGEESYYQTLSQRALERLGNPVEEVKLSKPS
                     AGAEADPALGAEINFHLTRARELTALRFHGLAVTELDQVNRRANPKNRLQPLLMREYF
                     ANQAYGRSLNLASQLPISQSERNYYRYPLAYWELVQQKASERELDPHLILALIRQESL
                     FDARARSPATALGLMQMIPPTAARVAKQLGLPAPTQEKLFDPELNVNLGTQYLKDLMQ
                     RYSNNWFKAIAAYNAGEAAVDRWEREIATDDIEEFVERIPYIETRGYVKLVLRNHRIY
                     KRLYEP"
     gene            2946..3377
                     /locus_tag="VMZ02_07685"
     CDS             2946..3377
                     /locus_tag="VMZ02_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HUR71851.1"
                     /translation="MKIEGSYMFNAPRERVWQLLLDPKIMAQCMPGCEAMNEVAPDRY
                     ETVMKVGVASVKGTYKGKVAIKDKQPPAHYVLSGEGSGGPGFMQGDVIIDLEESNGQT
                     VLKYSTEPKIGGLIASVGQRMLNGVAKMMADQFFKKVESFV"
     gene            3421..4326
                     /locus_tag="VMZ02_07690"
     CDS             3421..4326
                     /locus_tag="VMZ02_07690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HUR71852.1"
                     /translation="MEPGVSVIIPTYNRWPVVGAAVQSVLAQSYEDFELIVVDDGSTD
                     QTAAQLAKFGVRLRYFYRDRGGVAAARNLGVRQSRGRLIAFLDSDDLWHPDKLKIQAQ
                     FMASSPEVQICQTDEIWLRNGVRVNPRIKHRKPSGEIFERSLELCLISPSAVMMSRDL
                     FDRSGGFDEMLPVCEDYDLWLRIARDYPVPLIEQPLVVKHGGHADQLSHSTWGMDRYR
                     VVALLKLLRSGISGKRRAAVMRVMQKKITVLAGGACKRGKAADAERYRATLSEFAGEI
                     DDVGERVPQFRRVQGIPCTDAEPMA"
     gene            4346..4819
                     /locus_tag="VMZ02_07695"
     CDS             4346..4819
                     /locus_tag="VMZ02_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71853.1"
                     /translation="MALAKLSLELKPSENYLRDLMDWSEEIALRDGIAIAEIFASKWV
                     YDIATNPRLGRADKVKRIRESLRRMRFPRLARIEDEIQQKIHLLKLQPEIRMTVPRGL
                     EGGRLQIDFSVTNQKDLKRLADKLAAVAETNVTGEIFTLLGGVAGGSEPVSGRQT"
     gene            4819..5916
                     /locus_tag="VMZ02_07700"
     CDS             4819..5916
                     /locus_tag="VMZ02_07700"
                     /inference="COORDINATES: protein motif:HMM:NF045247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71854.1"
                     /translation="MQNFLPEEVIVEHSSEASPIYKNLRRALPDVPFRFIDDYASANG
                     SANLPSDSFGSAKKKLFLAKHKGEFLKKCPGSEGQVCCNYFVINFASNCPMDCSYCYL
                     QEYLADNASLKVFSNVGDLIDEADATLRKHRGLFFRIGTGEITDSLALEPYTGMVREI
                     LPYFAEQPNVLLELKTKSDCVDDLLDLDSKGRVVVSWSMNPQRVIHADEHATASLTER
                     LNAARRVQDAGYRLGFHFDPMIEYPGWETDYHTMLEQTFAAIDWRKLSWVSLGVLRDT
                     PGLKRTMRQRFSRSQLLTGEQVLCPDGKLRYYQPLRVEMYRKMVGWIRHAAPLVKVYL
                     CMESREVWEQVFGYAPSCEKELGNQFGSLTI"
     gene            5930..6796
                     /gene="murI"
                     /locus_tag="VMZ02_07705"
     CDS             5930..6796
                     /gene="murI"
                     /locus_tag="VMZ02_07705"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HUR71855.1"
                     /translation="MGTSLVAKLMPSPRAKIGDLKSKIDKDSAIGVFDSGIGGLTVLH
                     QIIEALPQENTVYLGDTARSPYGTKSVETVMRYSFENSAFLVEKGVKLVVVACNTSTA
                     IALSRLRETLTVPVLGVIEPGVRRAIKSTKNKKIGVIGTEATIQSGAYTRALKAADSN
                     VEVYSRACPLFVPLVEEGWTDNSVVEMTVKAYLGSLKQSGIDTLVLGCTHYPLLKKAI
                     RKFLGNGVLLIDSAEETAIEVAATLKKRSRQRKSGRAVHSFFVTDAPERFVKVGRRFL
                     GENVESAVRIER"
     gene            6913..>8327
                     /locus_tag="VMZ02_07710"
     CDS             6913..>8327
                     /locus_tag="VMZ02_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71856.1"
                     /translation="MWDFIKKIVGTKNDREIKRIRPFVDVINNLEPEYQRLSDDELKA
                     KTEQFKGRLAEATATLKSELEDTQNRASVADPEEREELKTQAEELDKELRRKEAELLD
                     QLLPEAFAAVREASRRTIGLRHYDVQLIGGAVLHSGKIAEMKTGEGKTLVATLALYLN
                     ALTGRGVHLITVNDYLARRDVQWMGPIYHKLGLSAASIVHEVSYLFDPTYLTKDYRFL
                     NLRPISRQEAYQADITYGTNNEFGFDYLRDNMKFSLDEYVQRELNFVVVDEVDNILID
                     EARTPLIISGPAEESTDKYYLIDRVIPKLQRGAVIQGDPSQEDRAAIEAKGDYTVDEK
                     SRSVTLTETGVAKVERLLGVHNLYDPRQIDTLHHVQQALKAHAIFKRDVDYVIKDGEV
                     IIVDEFTGRLMPGRRWSDGLHQAVEAKERVHIREENQTLATITIQNYFRMYKKLAGMT
                     GTADTEAPEFQKIYKLDVTVVP"
BASE COUNT         1875 a         2118 c         2406 g         1928 t
ORIGIN      
        1 ggcgctggca attagggcgg caaaaatcga cctctggtgc acctggcgcg gcgcaagcac
       61 ttccgggtgc cactgcacgc caatgacgaa agtatggccg gtgctttcga tgccttcgat
      121 gatcccatca ctcgcaatgg cgttaatgac caagccgcgg cccaatgtct tcaccgcctg
      181 gtgatgtgtt gtattgacct cggtatgagt gcgtctgatg attgcgtgca atcgcgtgtg
      241 tggttggagc gtgatgcgat ggccgcgctt gatggctttt ttactttgct ggtgctcctg
      301 agcatttggc acctgcgcgc cgatatcttg gtaaaggctg ccgccctgca cgacgttgat
      361 cgcctgggcg ccgccgcaaa cgcccaaaac cgggaggtcc tgtttcaatg ccacggcggt
      421 gatctccagc tcgaaagcgc tgcgttccgc cttgatggcc ccgagagctg ggatcggtat
      481 ttcgccgtaa taacttggat ggatatcgaa gttgccgccg ctcaatatca agccgtcgat
      541 acgcgccagt agggcgacga tggccccgct cgatggcatc ggtgggagga taactggaac
      601 ggcgccggaa tcagccactg ctttgatata gcgttgggcc agaaaataga gcgactcgcg
      661 gttttctccc cggccggcaa ctgaaatatc agaagtgatg ccgatcagcg gagccgggtg
      721 tttcattatg gtcagggttc gtacaggcgt ttatagatgc ggtggttacg gaggacgagt
      781 ttaacatagc cacgagtttc gatgtagggg attctctcga cgaattcttc gatatcgtcc
      841 gtggcgattt cgcgttccca acggtcgact gcagcttcac cggcattata agcggcgatg
      901 gctttgaacc aattattcga gtagcgctgc atcaagtctt tcaaatattg cgtgccgagg
      961 ttgacgttaa gttcggggtc gaacagtttt tcttgtgtcg gtgccggcag accaagttgt
     1021 ttggcaacgc gagcggcggt cggaggaatc atttgcatca gccccaaggc agtcgccggc
     1081 gaacgggcgc gggcatcgaa tagactctct tggcgaatca gtgcgaggat caaatgtgga
     1141 tcgagctcgc gctccgaagc tttttgttgc acgagctccc aataggcaag aggataacga
     1201 taatagttgc gttcgctttg tgaaatcggc aactgactgg ctaaattaag gcttcgacca
     1261 tacgcttgat tggcgaagta ctcgcgcatc aataggggct gcaagcgatt cttcggattg
     1321 gcgcgccggt ttacttgatc gagttcggtt acggccaagc catggaatcg caaggcggtg
     1381 agttcgcgcg cccgcgtcaa gtggaagttt atctcagcgc ccaaggccgg gtcggcttcc
     1441 gcgcccgcgc tgggctttga cagcttcact tcttctacgg gattgccgag ccgttccagg
     1501 gcacgttggg acaaggtttg ataataggac tcctcaccgc tgttaaaaat ttgccggtaa
     1561 atctgtttgg cagtgtcgac atcgccgagc ttttccgtac tgcgcgcttg ccagtaaacc
     1621 gccgcggtgt caaacgtgct gctccgagac tgcgccgcca gcgccttaaa cgtcgtcaac
     1681 gcagcttgcc agtcgccgct acggtaatga agccaggcca agcgccagac tgcgtcgtca
     1741 cgaacagtgc tattgggaaa ttgtttttgc accgtggtgt agagctggat cgcttccgag
     1801 tttctgccga agtactcgtg aatgtccgcg gcggcgaagg tggctcgatc agcgtaggcg
     1861 ctgctagagt agcgctgcac cagctggttg aaatattcca atgcctgggc gttctcgtgg
     1921 cggttccaca gggtctgacc gatctgatag agagctttgg gcgcttcgct gctgtcggga
     1981 aaattacggg caatctgttc caataccgcg atcgcctcgt tgcggccacg cacggcggct
     2041 agtttagtta gataacggag gcgttggtcg ttgtcggtga cgctgtctaa tatacgttta
     2101 tagagttcgg cggcgtcatt gagttggcgc tcgcgtgcca accgatcggc ttcgtcgcca
     2161 agagctgcga ttgtgctcaa tgcgaattgc tcgggatatt tgtcgcgcaa gcgagtctga
     2221 tctttccgtg ctaagcccgc ccaacttgaa gtcggactgt tgtcgcgcac ttcctggtaa
     2281 ccgtagtacg cgcgctgtac gtctccttgt gcctcaaaat tttgtgccag caaataactc
     2341 gcttcgtcga caatctcgcg tggcgtcccc ttgtctgcgc gcagttggcg caggatctca
     2401 ttcgcttgag ctagcttttt ctcagcctgg tcaattttcg cccgctgcaa agtagcccgg
     2461 aattgccaaa ctgattgcgg gtaacgctgc ttcagctgca gcaagtattg gcgtgattgc
     2521 tcccaatttc tctctttgaa cgcgattaaa gccaagtaat gcaggctata gtcgctaagc
     2581 cggtaattgg cctccaaagt tttgtgaaaa agctccttgg cttctgttgt gttaccttgg
     2641 ttgaagagtg cgtaagcttt gcgaaatgcg ctgcggccac tgtcgttttg agcgtctact
     2701 cttgcccatc ccagacacag cagaatcaat gctgcgcaat accagattgt ctgcttcatc
     2761 agtgtcttga gtgtaaacaa gcgcacgtct cgattcaatc agatttttcc gaaaacgggg
     2821 tccgtgccgg gtgcttttaa tctgttgtgt tttagacgca tgaacgttat gtttttcacc
     2881 caactacatt gcggcccgag cggacggctc gacgtggata tcgcgactaa ggagagacgg
     2941 cgacattgaa aatcgaaggc agttacatgt tcaacgcgcc tcgggaacga gtctggcaat
     3001 tattacttga tccgaagatc atggctcaat gcatgccggg ttgcgaagcg atgaatgaag
     3061 tggcgccaga ccgttacgag accgtgatga aggtcggcgt ggcttcggtg aaagggacct
     3121 acaaaggcaa agtagccatt aaagacaaac agccgccggc gcattatgtt ttgagcggcg
     3181 agggctcggg cgggccgggt ttcatgcagg gtgatgtaat tatagatctc gaagagagca
     3241 atggtcagac agtgctcaaa tacagcacgg agccgaagat tggtgggctc atcgccagcg
     3301 tcggtcagcg tatgctcaat ggcgtcgcta aaatgatggc cgatcagttc tttaaaaaag
     3361 ttgagagttt cgtctgaatc ctcgcaccgt ctgtctcggc tgatctgtgc aaaaaactca
     3421 atggaacccg gcgtcagcgt tataattccg acctacaacc gctggccggt agtaggcgcg
     3481 gcggttcagt cggttttggc gcaaagctac gaagattttg agctaatcgt tgtagacgac
     3541 ggttccactg atcagaccgc cgcgcagttg gctaagttcg gggtacggtt gcgctacttt
     3601 tatcgagatc gaggcggggt ggcggctgcg cggaacttgg gcgtgaggca aagtcgcggt
     3661 cggctgatcg cgtttctcga ttcggacgat ctctggcatc cggacaaact caagatccag
     3721 gcacagttca tggcaagctc gccggaagtg cagatctgcc aaactgatga aatttggctt
     3781 cgcaacgggg tacgagtcaa tccacggata aagcatcgca agccgtcggg agaaattttt
     3841 gagcggagtc tcgagctttg cctgatcagc ccgtcggctg tgatgatgag ccgtgacttg
     3901 ttcgatcgga gcggcggctt cgacgagatg ctgccggtct gcgaagacta cgacctgtgg
     3961 ctgcgaatcg cccgtgatta cccggtgccg ttaattgaac aaccgttggt agtcaaacat
     4021 ggcggccatg ctgaccagct ttcccattca acgtggggta tggatcgtta tcgcgtggtt
     4081 gctttgctaa aacttttacg atccggtatc agcggcaagc ggcgagctgc agtgatgcgg
     4141 gtcatgcaaa agaaaatcac cgtgctcgcc ggaggggctt gcaagcgcgg caaagcggca
     4201 gacgccgagc gctaccgagc aacgcttagc gagtttgccg gggagattga cgatgtcgga
     4261 gagcgcgttc cgcagtttcg cagagtccaa gggattccat gcacagacgc tgagccgatg
     4321 gcttagttgg gaagtcgatg atcggatggc actagccaag ctttctttgg aactaaaacc
     4381 cagcgagaat tatctccgcg acttaatgga ttggagcgag gaaatcgcct tgcgtgatgg
     4441 cattgccatt gcggaaatct ttgcgagcaa gtgggtctac gacattgcca ccaacccccg
     4501 attgggtcgt gccgacaagg tgaagagaat acgggaaagt ttgcgccgga tgcgctttcc
     4561 gcgcttggct cggattgaag atgaaatcca acaaaaaatt catctgctca agctgcaacc
     4621 cgaaattcgc atgacagtgc cacggggctt agagggcggc agattgcaga ttgacttcag
     4681 tgtcaccaat cagaaagatt taaagcggct ggccgacaag ttggcggcgg tagccgaaac
     4741 gaatgtgacc ggtgaaatat tcacgctcct gggcggagtc gcgggcggtt ccgagccagt
     4801 ttcgggacgg cagacttgat gcaaaatttt ctgcctgaag aagtgattgt cgagcatagt
     4861 tccgaagcta gcccgattta taaaaatttg cgccgcgctt taccagacgt gccatttcgc
     4921 tttatcgatg actacgcttc cgcgaatggg agtgcgaacc tgccgagtga ttccttcggc
     4981 tcagccaaga aaaagctttt tttggccaaa cacaaaggcg agtttttgaa aaaatgtccc
     5041 gggagcgaag gccaggtctg ctgcaactac tttgtcatca atttcgccag caactgtcca
     5101 atggattgca gctactgcta tctccaggaa tatttggccg ataatgcgtc cttgaaagtc
     5161 ttcagcaatg tcggcgattt gatcgacgaa gccgacgcga ccttgagaaa acatcgcggg
     5221 ttgttttttc gcatcggtac cggggagatt accgacagtt tggccctgga gccctatacc
     5281 ggcatggtgc gcgagatttt gccgtacttt gccgagcaac cgaatgtttt gttggagctc
     5341 aaaaccaaaa gcgactgtgt ggacgatttg ctcgatctcg attccaaagg gcgcgtcgta
     5401 gtgagttggt cgatgaaccc tcagcgcgta atccatgccg atgagcacgc caccgcttcc
     5461 ctcacagagc gactgaatgc cgcgcgccgt gtacaagatg ctggctaccg gttgggcttt
     5521 cattttgacc caatgatcga gtatccgggt tgggagactg actaccatac tatgctggaa
     5581 caaactttcg cggcgatcga ctggcgcaag ctctcctggg tgagtctagg cgtgttacgc
     5641 gacacgccgg gtttgaagcg caccatgcgg caacgctttt cacgaagcca actgctgacc
     5701 ggtgagcaag tgctgtgtcc cgacggcaag ctgcgatatt accaaccctt acgagtggag
     5761 atgtaccgaa aaatggtcgg ttggattcgc cacgccgcac cgctggtgaa ggtctatctt
     5821 tgcatggaat cccgcgaagt ctgggaacag gtattcggct acgcgccctc ttgtgaaaaa
     5881 gagcttggca accaattcgg cagcttgact atctaatccg cagggtcgcg tggggacgtc
     5941 gctagtcgct aaactgatgc cttcaccgcg agccaagatc ggggatttga aatcgaaaat
     6001 cgacaaagat agcgccatcg gggtttttga ctctggcatt ggcggcctca cggtgttgca
     6061 ccagatcatt gaggcactgc cgcaggagaa tacggtgtat ttgggtgaca cggcgcgctc
     6121 cccctacggc accaagtcgg tggaaaccgt tatgcgctac tctttcgaga acagcgcatt
     6181 ccttgtcgaa aagggagtca agctggtggt cgtcgcttgc aatacgtcga cggccattgc
     6241 gcttagccgc ctgcgagaga ccctaaccgt gccggttctc ggtgtgatcg agcccggtgt
     6301 gcgaagggct attaagagta cgaaaaataa aaaaataggc gttatcggta ccgaagcgac
     6361 gatccagagc ggcgcctata cgcgtgcatt gaaagcggcc gattctaacg tcgaagtcta
     6421 cagccgggcc tgtccgctgt ttgtgccgct ggtggaagaa ggctggaccg acaactcggt
     6481 ggtcgagatg acggttaagg cttatcttgg cagtctcaag caaagcggca tcgatacctt
     6541 ggtattgggc tgcacccact atccactgct aaaaaaggcc atccgaaagt ttctcgggaa
     6601 cggcgtgctt ttgatcgatt ccgctgagga gaccgccatc gaagttgcgg cgacactcaa
     6661 gaaacggtcg aggcagcgaa aatccggccg ggcagttcat agtttctttg ttaccgacgc
     6721 gccggaacgc ttcgtcaaag tgggtcgccg ctttctcggc gagaacgtcg aatcggccgt
     6781 gcgcatcgag cgctaaggtt ccggcaacac cagatcacct ttaagtcgcg tgcagattcg
     6841 tgtattttag tgctatcctt agtagccaaa tggctcctcc gcttgcagca aatccgggag
     6901 taaagttcta agatgtggga ttttattaaa aagattgtcg gcaccaaaaa tgaccgtgag
     6961 attaagcgca tccgtccctt tgtcgacgta atcaacaatc tcgagccgga ataccagcgc
     7021 ctcagtgacg acgaactcaa ggcgaaaacc gagcagttca agggccggct tgccgaagct
     7081 acggcgactt taaaaagcga gttggaggat acgcaaaacc gcgcgagtgt cgccgatccc
     7141 gaggagcgcg aggaactcaa gacccaagcc gaagagctcg acaaagaact tcggcgaaaa
     7201 gaggcggagc tcctagatca gttgctgcct gaagcgtttg ctgcagtgcg cgaagcctcg
     7261 cgtcgcacca ttggcttgcg tcattacgat gtccagttga tcggcggggc ggtgcttcac
     7321 tccggcaaga tcgctgagat gaagaccggc gagggcaaga ctttggtcgc gactttggcg
     7381 ctttacctca acgcgcttac cggccgcggt gtgcacttaa ttaccgtaaa cgattacttg
     7441 gcgcggcgcg atgtccagtg gatgggaccg atttaccaca aactcggttt gagcgccgct
     7501 tcaatcgtgc acgaggtcag ttaccttttc gatcctacct atctgaccaa ggactaccgt
     7561 ttcttgaatc tgcggccgat ctcgcgccag gaagcttatc aggccgatat aacctacggc
     7621 accaataacg aattcggatt cgattatttg cgcgacaaca tgaagttttc gctggacgaa
     7681 tatgttcagc gggagctcaa tttcgtggtc gtcgatgaag tggacaatat tttaatcgac
     7741 gaggcgcgta caccgttgat tatctccgga ccggccgaag agtcgaccga taaatactat
     7801 ctgatcgatc gggtgattcc caaactgcaa cggggcgcgg tgatccaggg cgatccgtcg
     7861 caggaagatc gcgccgccat cgaagccaag ggcgattaca cggtcgatga gaaaagccgt
     7921 tcggtgacgt taaccgaaac tggtgtggcc aaggttgaac ggctcctggg tgttcataac
     7981 ctgtacgacc cgcggcaaat cgataccctg catcatgtac agcaggctct caaagctcac
     8041 gcgattttta agcgcgatgt cgattacgtc atcaaagacg gtgaagttat catcgtcgac
     8101 gaatttaccg gccgattaat gccggggcgg cgttggtccg atggtttgca ccaagcggta
     8161 gaagccaagg agcgcgttca tattcgcgag gaaaaccaaa cgctcgcgac catcacgatt
     8221 caaaactatt tccgcatgta caagaaactc gcgggcatga cgggcactgc cgacaccgaa
     8281 gctcccgagt ttcaaaaaat ttataaactc gacgttacgg tggtgcc
//
LOCUS       DATLCH010000261         3505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2933148, whole genome shotgun sequence.
ACCESSION   DATLCH010000261 DATLCH010000000
VERSION     DATLCH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2933148"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VMZ02_07715"
     CDS             <1..418
                     /locus_tag="VMZ02_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71857.1"
                     /translation="TVAMDKLIIGAYETSLANDELLVGVEIPVLSKSQRAAYLKFQLK
                     ERPTLGLALVLELEGENIKTASAVVGSVSAAPTRSSKANDLLTGARAQVEKQLGDAAE
                     ALAQAADPVDDLEGGADYKRHLIGVFLRRAFTKALS"
     gene            679..1125
                     /locus_tag="VMZ02_07720"
     CDS             679..1125
                     /locus_tag="VMZ02_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71858.1"
                     /translation="MAGEKTIGDITFRIRHTMMLVSDLDRSIDFYTRLLGMDVQRLRP
                     SPDKNERVGYLGYGTEDEYPGLELIETGGPGHVPAMPKWYGHVALYVSDLYKLSEKLK
                     AAGVKFTLGPQPNRPGSQDHVAFIQDPDGYLLELTERHSKSGAPLG"
     gene            complement(1143..2147)
                     /locus_tag="VMZ02_07725"
     CDS             complement(1143..2147)
                     /locus_tag="VMZ02_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71859.1"
                     /translation="MMTVLIAAISFFLSLSAPSHGADADAQRLLVEAARKEAKMIFYT
                     SVETEFARALTGAFEAKYPFIKTDIFRSTHDRIFSRMNIERQTSTYVADVVSVGEFET
                     YQMQKKGFLQPYKSPSAVVYPEGFKDPAGYWTDLYDNLIVTAYNTNRVKREELPKRWE
                     DLLHPRWKGRMVLDQNEDRWFANMLHFMGERKGVEFMQALAKQEVAIRSGRSLITQLM
                     SAGEFDLQIVAYWYRPYLMKKQGAPVDWLGLEPAIVALHPISIVEKAPHSGAARLFID
                     FALSDEGQRVFLKRGREPALPGLRPEGYPSHLKMIPSRVQLAEKLDDYSRQYRALFVR
                     "
     gene            complement(2266..2877)
                     /locus_tag="VMZ02_07730"
     CDS             complement(2266..2877)
                     /locus_tag="VMZ02_07730"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71860.1"
                     /translation="MSSKTIYVDLDDVLCETARHFLFIIEREFNKRIAYDQLTDFDVG
                     LSCGLRPAQAEELYRIIHRTDELLQIAPIDGAISTLHQWAADGYEIAIVTGRPPDSYE
                     SSLEWLSRYRVPYESFSMVDKYSRFATENTVAIHLDELAQREYCWAVEDSLPMAQFLA
                     TRMQVPVALIDCPWNRTSTDHVNVGRYGDWPTLAQQMAKIRPR"
     gene            complement(2874..3347)
                     /locus_tag="VMZ02_07735"
     CDS             complement(2874..3347)
                     /locus_tag="VMZ02_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71861.1"
                     /translation="MDKYIIRIDSTPERLIEAGPLVRGSWSREHDQLGREIRGEFLID
                     TGAYGAMIDLAVAETLALSPRGTKSVHGIHGYGTLQLYLGRVSLPAVDAAGNQTVYST
                     VLECTGVPSLREKSFEHRADVIGILGRVFLRNARITVDNPAGNLELLIANRTEES"
BASE COUNT          788 a         1009 c          951 g          757 t
ORIGIN      
        1 aaccgtggcc atggataaac taatcatcgg cgcctatgaa acttctctgg ctaacgacga
       61 gctgctggtc ggtgtcgaga ttcctgtgtt aagcaaaagc caacgtgccg cgtatctgaa
      121 attccagcta aaggaacggc ccactttggg attggccctg gtcctggaac tcgaaggcga
      181 aaatatcaaa acagcttccg ccgtggtcgg cagcgtcagt gcggcgccta ctcgatcttc
      241 caaggctaac gacttactta ccggtgcccg agcgcaagta gaaaaacaac tcggcgatgc
      301 cgcggaagct ttggctcaag ccgccgatcc ggtcgacgac ctcgaaggcg gtgccgacta
      361 caagcgccac ttgatcggcg tgttcctgcg gcgcgctttc accaaagcgc tgtcctaacc
      421 cgtctcccgc tactgccaac gcgtcaaagc cggcggtagc aaatagcagc ttgtgcagcc
      481 gtccaacttt atacgctgcg atttaccttc cgcgcccatg gcccggtcgc cagtgtcgcg
      541 tgcatcgtta aattttcact ggtgaaaaat agttcgtgct ccggctcgtg cttgattcca
      601 cgcgcgcgcc tgggctagtg tcggcacaaa gcgcgtcgct gtatcgagtt gaatttgcct
      661 aagcatcagg agaaaaccat ggccggagaa aaaaccatcg gcgatatcac ctttcgcata
      721 cgtcacacga tgatgctggt cagcgatctc gatcgcagca tcgattttta taccaggctg
      781 ttaggcatgg acgtgcagcg cctgcgccct tcgcctgaca agaacgagcg cgtgggctat
      841 ctcggttacg gcacggaaga cgaatatccc ggtttggaac tgatcgaaac cggcgggccg
      901 ggccatgtgc cggcaatgcc caagtggtac ggtcacgtgg cgctgtacgt ttccgatctc
      961 tataagttga gcgaaaagtt aaaagcggcc ggcgtgaagt tcaccctggg accgcagccc
     1021 aatcgtcccg gcagccaaga tcacgttgcc ttcatccagg accccgacgg ctatcttctc
     1081 gagttaaccg agcgccattc gaagagcggc gcgccccttg gttagaggag tttgccagcc
     1141 gctcagcgca caaacaaagc gcgatattgt cggctgtaat cgtcgagttt ctcggctagc
     1201 tgcacacggc tgggaatcat tttaagatga ctgggataac cctcgggtct aagaccaggc
     1261 agcgccggct cgcggccgcg ctttagaaag accctctgcc cttcgtcaga tagcgcaaag
     1321 tcgatgaaca aacgcgctgc gccggagtgc ggcgcttttt cgacgatact gattggatga
     1381 agcgcgacga tcgccggctc caacccgagc caatcgaccg gcgcgccctg ttttttcatc
     1441 agatacggcc ggtaccaata agcgacgatc tgcaagtcaa attcgccggc actcatcagt
     1501 tgggtgatca gcgagcggcc gctgcgaatg gcgacttctt gcttcgcaag cgcttgcatg
     1561 aactccacgc ctttcctctc gcccataaaa tggagcatgt tggcaaacca gcgatcttcg
     1621 ttctgatcaa gcaccatgcg acccttccag cgcggatgga gcaaatcctc ccagcgtttc
     1681 ggcagctcct cgcgtttcac tcggttggtg ttgtacgctg taacgattaa gttgtcgtag
     1741 agatcggtcc agtaaccggc ggggtctttg aacccttcgg gatagactac ggcactgggc
     1801 gatttgtatg gctggagaaa gccctttttc tgcatctggt aggtttcgaa ttcaccgacg
     1861 ctgacgacgt cggcaacgta ggtgctggtc tgccgctcga tgttcatgcg gctaaagatt
     1921 ctgtcatggg tggagcgaaa gatgtcggtc ttgatgaagg gatacttggc ctcgaaagcg
     1981 ccggtgagcg cgcgggcgaa ctcggtctca accgatgtgt agaaaatcat tttagcttct
     2041 ttgcgcgcgg cttcgacgag caaccgctgc gcatccgcgt cggcgccgtg gctgggtgcg
     2101 cttagactta ggaaaaaact aattgccgca atcagtactg tcatcatatt caattgcctc
     2161 cagcattcac gatgcacttt tacgcgaagc ggctctcacc actacataaa tcgatttcga
     2221 tggcccggtg ccataaaaaa taggcgccac aaaaaaactc agctgttagc gaggacgaat
     2281 cttcgccatc tgctgcgcca acgtcggcca atcgccgtaa cgtccgacgt tcacatgatc
     2341 ggtgctggtg cgattccacg gacagtcgat taacgccacg ggaacctgca tgcgagtcgc
     2401 gaggaattgc gccatcggca aggaatcttc caccgcccaa cagtattccc gttgggctaa
     2461 ctcgtcgaga tgtatcgcaa cagtattttc cgtcgcaaaa cgtgaatact tgtcgaccat
     2521 gctgaaggat tcgtatggca cgcgatagcg cgacagccac tctaaagaag actcgtaaga
     2581 atccggcggc cggccggtca caatagcgat ctcgtaaccg tcagccgccc attggtgaag
     2641 agtcgagatt gcgccgtcaa tcggagctat ctgtaaaagc tcatccgttc gatggatgat
     2701 acgatataac tcctcggcct gcgctggccg gaggccgcaa gacaaaccca cgtcgaagtc
     2761 ggtcagttgg tcgtaagcga tccgtttgtt gaattcacgc tcgataataa ataaaaaatg
     2821 gcgcgcggtt tcgcacaata cgtcgtcaag atcgacgtag atggttttcg agctcatgat
     2881 tcttcagtgc gattcgcgat tagcaattcg aggtttcccg caggattgtc gaccgtaatt
     2941 ctggcgttgc gcaagaacac tcggccgaga atgccgataa catcggcacg atgctcgaaa
     3001 cttttctcgc gcaacgatgg cacacctgta cactcgagca cggtggaata caccgtctga
     3061 ttgcccgctg catcgaccgc cggcagcgat acccgcccca ggtaaagttg tagcgtgcca
     3121 taaccgtgaa tgccgtgcac cgactttgtc cctcgcggcg agagtgctaa cgtctcggcc
     3181 acagcaagat cgatcatcgc gccgtaagcg ccggtgtcga taagaaactc gcctctgatc
     3241 tcgcggccca actggtcgtg ctcccggctc caactgccgc gcaccagcgg cccggcctcg
     3301 atgagcctct caggggtgct gtcgatcctg attatgtact tatccatcac gctcacgaaa
     3361 gcagccgcat cagcgcttgg aaacgaaatg actgtcgcga accgaaaacc gtaacagctc
     3421 atccttccac ttgccggcat aatcgacacc gatgcgcggg gtggcgtcga actcgggcgt
     3481 cgcttcaccg cggtcttcga gataa
//
LOCUS       DATLCH010000262         7057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_701314, whole genome shotgun sequence.
ACCESSION   DATLCH010000262 DATLCH010000000
VERSION     DATLCH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701314"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VMZ02_07740"
     CDS             complement(<1..1018)
                     /locus_tag="VMZ02_07740"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HUR71862.1"
                     /translation="MSTDQLRSLNILVVDDSAVVREVMNAVLSQEPGFKVMVAGDPLI
                     AMGKMKNQRPDVIILDLEMPRMDGLTFLRKIIREDSLPVIICSALTGADTSIGLQALE
                     LGAVDVVTKPRLGVRDYLHESAIILIDAVRAAARAKAFTSVRSTVSPTHRFNAATRPI
                     KKWASPRPLTSEKVVAIGASIGGTEALRIVLESMPSNGPGIVVVQHMPEEFTKAFAQR
                     LNQLCQIEVKEAANGDRIMPGRALIAPGNFHTTVRRSGAQYVVEVTDGPLVSRHRPSV
                     DVLFRSVAQSVGPNSVGVIMTGMGNDGAEGLLEMKRAGAYTIAEDEASCVVFGMPKEA
                     IERGA"
     gene            complement(1015..1959)
                     /locus_tag="VMZ02_07745"
     CDS             complement(1015..1959)
                     /locus_tag="VMZ02_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HUR71863.1"
                     /translation="MNQPTSAPTQPAVDNSTRPMSDREFALFQKLIYRNAGIHLTPVK
                     TALLEARLTKRLRELGLKSFGEYYRYILEVDHSEELVVLLDRISTNETHFFREPKQFE
                     FLVNEVFPAWKNRNRSANGSRYLRIWSAACSSGEEPYSLAMTLWDHFPPGSGWEFEIL
                     ATDISTRMLDIASSGIWPIAKAGEIPQEYLKKYMLKGTGEHIGNMKAGAELRSVIRFQ
                     RINLNDDDYPVAGNLDLIFCRNVLIYFDAPSRARVIEHLVDRLAPNGFLFVGHAESLT
                     GVTDRVRYVVPTIYTVTDPSHTSHRLAAPKHFAVRTTR"
     gene            complement(2095..3555)
                     /locus_tag="VMZ02_07750"
     CDS             complement(2095..3555)
                     /locus_tag="VMZ02_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HUR71864.1"
                     /translation="MTSYIERRTLWADIEKRGVETADLLAKNIARDLFTFDQYKINGT
                     VAAFGNDPAIKYIEVKDKSGTVKAFRGDTRDLTATWAIVRPVFFNDAAAVGSVTLWIS
                     TASLEALLSRGWWSILLRETTNQVFLLVILTFLIRREISLPLSLVTARLREIAQGDCN
                     LTKRIEFNANNEIAAVALSFNQFVDKLTPVITQVHRSADAVALASHQLSASAEVLARG
                     TSEQAASVEETTSSLEQMSASISQNVDNTRQMEQMALAGAKQVEACAKAVSESVEAMN
                     SIAERITIVEEIASQTNLLALNAAIEAARAGDRGKGFSVVASEVRKLAERSQRAAQEI
                     SSLTTSSLNVAERSGQLLNELVPAIGKTVELVQEVSTASREQGAGVYQVNQAMTQVER
                     VTQTSAAAAEELTSTAGQLAARAVELQTLMSSLQIYPEVSEPGQLAVPAAAQSLHNKW
                     QPSAKATSRHAPSLKSGAGNEMRPPDHDHDRDYQRF"
     gene            complement(3754..4992)
                     /locus_tag="VMZ02_07755"
     CDS             complement(3754..4992)
                     /locus_tag="VMZ02_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HUR71865.1"
                     /translation="MLRIFQRQLTIRNKFYLLSALYALGYAVCGALAVQSASLSIIIA
                     VGVGFTTVNCFAAWYLADSIARPLHNFANRFTNHNHGQSGLLRWVKAIDRRDELGEVA
                     RAYNTFADNLYESIEGVIGTADSLSEASREIAASAQNLTQATSEHAAAVQETTASLEE
                     MNASITQNAENSRQMEQMALKGSKDIAESSKAVAETVKAMKTIAEKISIIEEIAYQTN
                     MLALNAAIEAARAGEHGKGFAVVASEVRKLAERSQIAAGEISSLTSSSVRVAEHSGEV
                     LVELVPSIRKTAELVQEVATASREQAMGVAQVNQAMTQVDRVTQRNAAWAEELSSTAD
                     QMAAHAETLQRLMKVFRVDRSAPSSSATVNTSQASIRRVQENHRQIRPDQSSGERQSP
                     KTVDSTYGASFKPSQWTNEL"
     gene            complement(5194..5724)
                     /locus_tag="VMZ02_07760"
     CDS             complement(5194..5724)
                     /locus_tag="VMZ02_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HUR71866.1"
                     /translation="MTTMHDQESRIDTTQYLTFRLDGEEYAVGILQVREIIAYGTLTK
                     LPHTPSSIRGVINLRGNVVPVADLRIKFGLTAGRVTNRTCIVIVEANINENPTVMGVI
                     ADSVSQVIRMPEENILPAPAFGTRVCADYLLGMGRSGDKFILMLDIGKVLSADDPIPA
                     AREFNAAAPNTVETFK"
     gene            complement(5739..>7057)
                     /locus_tag="VMZ02_07765"
     CDS             complement(5739..>7057)
                     /locus_tag="VMZ02_07765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HUR71867.1"
                     /translation="NHQPGDSPRRSFGRRQADAKNWMQRAGTIRVDTKKLDRMLDLAG
                     EIAIAQGRLRQALEEAGKENPEALEAQDQVERLSLELQENIMKVRMVPVGPTFRQYIR
                     TVRDVAQDHGKCAMLSVMGEDVEVDVSIIEHLKDPLTHMIRNALDHGIEQPETRKTRG
                     KSPSGIISLKAFHEGANIVIQVSDDGAGLNRDKIVERAKLIGAVTEPEKLADPELYRM
                     ILEPGFSTADAVTDMSGRGIGMDVVRRNIEALRGSVGVESELGKGTTITVRLPLTLAI
                     IEGFAVGIGADTYILPLHAVSECLEMPVSEVAHNQAQGVIALRGEPLPYVRLRSWFEL
                     TSPRPPRENIVVVELDGAKTGLVVDSLQGARQTVIKSLGDQFKNLQGISGSAIMGNGR
                     VALIIDVSAVVREVIGSYGNGNVGRVFAKSDGNATRPTNVNSVSYH"
BASE COUNT         1463 a         1908 c         1959 g         1727 t
ORIGIN      
        1 ctgcgcctcg ctcgatggct tccttgggca ttccaaatac gacacaggag gcttcgtctt
       61 cggcgatcgt gtaggcaccg gcgcgtttca tttccaacag tccttcggca ccgtcattgc
      121 ccattcccgt catgatgacg ccgacggagt tgggcccaac actctgcgcc acagagcgaa
      181 atagtacatc gacgctcgga cgatgccgcg aaaccaaggg gccatcggtg acttcgacga
      241 catactgcgc gccgctgcgg cgcaccgtgg tgtggaaatt acccggcgcg ataagagcac
      301 gtcccggcat gatccgatcg ccgtttgcgg cttctttgac ctcgatttgg caaagttgat
      361 ttaatctttg ggcgaatgct ttggtaaatt cctcgggcat atgttggacc acgacaatgc
      421 ctggtccatt tgatggcata gactccagga ctatccgcaa tgcttcggta ccgccgatgg
      481 acgcgccgat ggcgacgact ttctcgctgg taagtggtcg cggggaagcc cacttcttta
      541 tcggtctagt tgcggcattg aaccggtgtg tcggactaac ggtggatcta acagatgtga
      601 aagccttggc gcgcgccgcc gcccgcaccg cgtcgatcaa aattatcgcg gattcatgca
      661 aatagtcgcg tacgccgagg cgcggcttag taacgacatc tacagcgcct agttcgagcg
      721 cttgcaagcc gatgctcgta tcggcgccag tcaatgccga acatatgatg accggaagcg
      781 aatcttctcg gattatcttg cgcaggaaag tcagaccatc catacgcggc atttcaaggt
      841 cgagaatgat gacatcggga cgctgattct tcatcttgcc catagcgatc aaggggtcgc
      901 ctgcgaccat gactttaaaa cccggttcct gagagaggac tgcgttcatg acttcgcgca
      961 cgaccgccga gtcgtcaacc acgagaatat ttaatgacct aagttgatcg gtactcatct
     1021 tgtagtgcgc accgcgaaat gtttgggtgc ggccagtctg tgcgaagtat gggatgggtc
     1081 ggtgaccgta taaattgtcg gcacgacata acgaactcga tccgtaactc ccgtcagact
     1141 ctcggcatgg ccaacgaaaa gaaatccatt aggtgcgagg cgatcgacca agtgctcgat
     1201 cacccgggcg cgcgacggcg cgtcgaagta gatcaggaca ttgcggcaaa aaatcagatc
     1261 gagatttccg gcgaccggat agtcgtcgtc attgaggttg attcgttgaa agcgaataac
     1321 cgagcgtagt tccgcgccgg ctttcatatt gccaatgtgc tctccggttc ccttgagcat
     1381 gtattttttt agatattcct gggggatctc accggctttg gcgatgggcc aaatcccgga
     1441 agatgctatg tctagcatgc gggtcgagat atctgttgcg agaatctcga attcccaccc
     1501 tgatcctggt ggaaaatgat cccacaacgt catcgcgagc gaatatggtt cttcgccact
     1561 cgaacatgca gcgctccaga tccggaggta gcggctgccg ttggctgacc ggttgcgatt
     1621 cttccacgcg ggaaagactt catttacgag aaattcaaat tgcttgggct cgcgaaagaa
     1681 atgggtttca ttggtggaaa tgcgatcgag taggacaacc agctcctcgc tgtgatcaac
     1741 ctccaaaata tagcggtagt actcaccgaa ggattttaag ccgagttcgc gcagacgttt
     1801 tgtcagccgc gcttctaaca aagctgtttt tactggagtg agatggatgc cggcgttgcg
     1861 gtaaattagc ttttgaaaca gcgcaaattc acgatccgac atcggacgag tggaattgtc
     1921 gacggctggc tgagttggtg ctgaggttgg ctgattcatg gtctcggctc ttgatacgaa
     1981 ctcatgcggg cagaacgttc ggtagattcg tacctcatga aagtttcagc ggtgcgatcc
     2041 ggcgatagcg caccgggccg ggtctctcct gggagcctcg cagtcgcctt tgcgttagaa
     2101 tcgttgataa tctcgatcat ggtcgtgatc tggcggtctc atttcattgc cggcgccgct
     2161 tttaagcgac ggagcgtggc gcgaagttgc tttcgccgac ggctgccact tattgtgcaa
     2221 agactgagct gcggcgggca cggccaattg cccgggctcg cttacctcag ggtagatttg
     2281 caacgaactc atcaacgtct gcagctctac ggctcgggca gcgagttgtc cggccgtact
     2341 cgttagctcc tcggccgctg cggcgcttgt ctgagtgact ctttccacct gtgtcattgc
     2401 ctgattcacc tgatagaccc cggcgccctg ctcgcgcgaa gcggtggaca cctcctgtac
     2461 taactcgacg gttttgccaa tggccggcac gagttcattc agcaactgtc cggagcgctc
     2521 cgcgacgtta agactagatg tcgttaagga gctaatttct tgagctgccc tttggctgcg
     2581 ttcggccaat ttcctgacct cggatgcgac cacggaaaac cctttaccac gatcacctgc
     2641 acgagcggcc tcaatggccg cgttaagggc aagcaggtta gtttgagaag cgatctcttc
     2701 aacgatggta attctctcgg cgatggaatt catcgcctca acggattcag aaacagcctt
     2761 agcgcatgct tcgacttgct ttgcgccggc cagagccatc tgttccattt gccgggtgtt
     2821 gtcgacattc tgactgatcg aggcactcat ttgctcgaga ctcgaagttg tctcctcgac
     2881 cgaggctgcc tgctcgctgg tgccacgcgc gagtacctct gcagatgctg agagttgatg
     2941 tgaagccagt gccacagcat ccgccgaccg gtggacttgg gtgataaccg gcgtaagttt
     3001 atcgacaaac tgattgaacg aaagcgcgac cgcggcgatt tcgttatttg cgttgaattc
     3061 gatccgtttg gtcaggttgc aatcgccttg ggcgatctct ctcaggcggg cggtgactaa
     3121 tgagagcggc agggaaatct ctcggcggat caaaaaggtc aaaatcacca gtaagaaaac
     3181 ttgatttgtc gtctcgcgca gcaagatgct ccaccagccg cgactgagca atgcttcaag
     3241 cgatgccgtc gagatccata aggttaccga gccaacagcc gccgcgtcat tgaaaaagac
     3301 cggacgaacg attgcccacg tagccgtcag gtctctagtg tccccgcgga aagctttgac
     3361 ggtccccgac ttatctttga cctcgatgta tttaatagcc ggatcattac cgaatgcggc
     3421 aaccgttccg ttgatcttgt attgatcgaa ggtgaacaaa tcgcgagcga tgtttttggc
     3481 taagaggtcg gctgtttcta cgccgcgttt ttcgatatcc gcccagagcg ttcggcgttc
     3541 tatgtagcta gtcacgatcg ttgaaatgac accgatgatc agcatcgcaa ggccggtaat
     3601 tagactaaaa cgaaaaacta aactgctctt gcgccgtcta cgcattggag cgaaatcgga
     3661 atagttcata aactttcact gcggcagtag tgcaagctag agacaaggca gccgctgatg
     3721 attttgcacc ttcatcgctg tgttcttttg tttctacaat tcgttggtcc actgggaagg
     3781 cttaaaactc gccccgtagg ttgaatcgac ggtctttggg gattgacgtt cgccgctaga
     3841 ttggtccggt cgtatttggc gatgattctc ctgaacgcga cgaatggagg cctggctagt
     3901 gttgacagtc gcgctggagc ttggcgcgga gcggtcgacg cggaacactt tcatgagtcg
     3961 ctgtagtgtc tcggcgtgcg cggccatttg atccgctgtg ctcgagagtt cttcggccca
     4021 ggccgcgttt cgctgggtta ctctatcaac ctgagtcatg gcttggttaa cctgggcgac
     4081 tcccatagcc tgttctctcg acgctgtcgc aacctcttgg accaattcag cggtcttcct
     4141 gatcgatggc accaactcta ccaagacttc tcccgagtgt tcggcaactc tcacgctcga
     4201 agacgtcaga gagctgatct ctccggcagc gatttggctt ctctccgcga gcttcctaac
     4261 ttcactggcg accaccgcga atcccttgcc atgctcgccg gcgcgcgctg cttcgatggc
     4321 ggcgttaaga gctaacatat tggtctgata ggcaatctcc tcgatgatgg aaatcttctc
     4381 ggcgatggtt ttcatagcct tgaccgtttc cgcaacggct ttgctgctct cggcaatgtc
     4441 tttggaaccc ttcagcgcca tctgttccat ctgccgcgaa ttctccgcgt tctgggtaat
     4501 agaagcattc atctcctcca aactcgcagt ggtttcttgc accgctgccg catgttcgct
     4561 ggtcgcttga gtgagattct gcgccgatgc ggcaatctct ctcgaagctt ccgatagcga
     4621 gtcggcggtg ccgataactc cttcaatcga ttcgtaaaga ttgtctgcga aggtgttgta
     4681 agcgcgggca acctcaccga gctcgtcacg acggtcgata gctttaaccc atcgcagcag
     4741 tccggactgg ccgtgattgt gattggtgaa acggttggca aaattgtgca acgggcgggc
     4801 gatgctatcc gccaaatacc aagcggcaaa acagttgacg gttgtaaagc caaccccgac
     4861 cgcgattatt atgctgagac tcgcgctctg tacggccagt gctccgcaaa ctgcgtagcc
     4921 aagggcgtaa agagcggaaa gcaggtaaaa tttatttcgg atcgtcagct gcctttgaaa
     4981 tatccgtaac atcgttacta ctctcctgtt gagtattgac tcgccgtctg agctctccgc
     5041 cgactctcaa ttagtcgtac attggtagtc gtacattggg ctgtttcaaa aagaccgagt
     5101 tttccctcgt gcgatatccg tcgattgctg ctggtaccaa tttcgcgtgg ccgtgctgca
     5161 ttggcgattg tcaggccgga tggtgtcatg ggcttactta aaggtttcca ccgtgttcgg
     5221 cgcggcagcg ttgaattccc gggccgccgg tatcggatcg tcagcactca agactttccc
     5281 gatgtcgagc attaagatga acttgtcgcc ggaccttccc atgccaagca aataatcagc
     5341 gcatactcgc gtgccgaacg ctggcgcggg caggatattc tcctcgggca ttctaattac
     5401 ttgactgacg gaatcggcaa taactcccat gacagtgggg ttctcgttga tattggcctc
     5461 gactatcaca atgcaggtgc gattggtcac tcggccggcg gtcaaaccaa attttatcct
     5521 aagatctgcg actgggacga cgttgccgcg taaattgatg acgccgcgga tcgacgatgg
     5581 agtgtgcggc agtttggtca aggtcccgta ggctatgatt tccctaactt gcaggatgcc
     5641 cacagcgtat tcttcgccgt ccagacggaa tgtcaggtat tgtgtcgtat cgatgcgact
     5701 ttcttgatcg tgcattgtag tcatctgcgg tcgtacgcct agtggtacga aaccgaatta
     5761 acattcgttg gtcgggtggc gttaccgtcg gatttggcga ataccctgcc aacattgccg
     5821 ttgccgtagg aaccaatcac ctcgcgcacc acggcggaaa catcgatgat tagcgccact
     5881 ctaccgttgc ccatgattgc cgaacccgag atgccctgaa gatttttgaa ttggtctccc
     5941 agggatttga tcacggtttg gcgggcaccc tggagtgaat cgacgaccaa gccggttttg
     6001 gcgccgtcga gttcgacgac gacgatgttt tcgcggggcg gccgagggct agtgagttca
     6061 aaccaactgc gcagacgaac gtagggcaac ggctcgccgc gtaaagcaat gacgccctgt
     6121 gcctggttat gcgcgacttc gctgaccggc atttcgagac actcactgac ggcgtgcagg
     6181 ggcagtatgt aagtatccgc accgataccg acggcgaagc cctcaattat cgccagcgtc
     6241 agcggcaagc ggactgtaat cgtcgtgcct ttgccgagtt cactctccac tcccactgag
     6301 ccgcgtagag cttcaatatt gcggcgcact acgtccatgc caatgccgcg gccggacatg
     6361 tccgtgaccg cgtcagcggt agaaaatcct ggttcgagaa tcatccggta gagctctggg
     6421 tcggcgagct tctccggctc cgtgacggcg ccgatcaatt tcgctcgttc aactattttg
     6481 tcgcgattga gtccagcgcc atcgtcgctc acctggatga cgatattagc gccctcatgg
     6541 aaggctttga gcgaaatgat gccgctggga cttttgccgc gggtcttgcg ggtttcaggc
     6601 tgctcgatgc cgtgatcgag agcattgcga atcatatgtg tgagcggatc tttgaggtgt
     6661 tcgataatgc tgacgtcgac ctcaacgtcc tcgcccatca cgctgagcat ggcgcatttg
     6721 ccgtgatctt gggcgacatc gcggacggtg cggatatatt gacgaaatgt cgggcccacc
     6781 ggaaccatgc gaactttcat gatgttttcc tgaagttcga gggaaagtcg ttcgacttgg
     6841 tcctgcgcct caagcgcttc agggttttcc ttgccagcct cttcgagggc ttgccgcaat
     6901 cgcccttggg cgattgctat ctcgccagcg aggtcaagca tgcgatcgag ttttttggta
     6961 tcgacgcgga ttgttccggc gcgctgcatc caattcttgg catcggcttg ccggcgtcca
     7021 aaggaccggc gcggcgaatc gcccggttgg tgattcg
//
LOCUS       DATLCH010000263         5401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10038499, whole genome shotgun sequence.
ACCESSION   DATLCH010000263 DATLCH010000000
VERSION     DATLCH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10038499"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VMZ02_07770"
     CDS             <1..369
                     /locus_tag="VMZ02_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71868.1"
                     /translation="ISGNMFNNDEFGDYVIYAAWPKYRVFIDGRTDMYGASRVREYLT
                     ISQAKDGWEELLEKYQLTWVFHEPTSMLSKILLERTDWRLIYSDKVANIFVKSLPQHE
                     HLIAKYGATQPYKETSSYTN"
     gene            459..1478
                     /locus_tag="VMZ02_07775"
     CDS             459..1478
                     /locus_tag="VMZ02_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HUR71869.1"
                     /translation="MDVNIGIIGLGYWGPNLLRNFHASPDSNVIICCDSQADRCRKFA
                     VQFPNFTYSQLAEDVINHPEVNAVAIATPVQTHYELAKQALLAGKHVLITKPMTDDLD
                     EARKLVELADKMKCILQVDHTFIFHPAVEKLKDIVTRGELGELYYFDSVRINLGLFQS
                     DVSVIWDLAPHDVSIMDYLIDRPVQWVQAIGVSHAGHKSESMAYITVQFAGNVLGHCH
                     VNWLAPMKTRLVMLGGSRRLAVYDDNLVTEKVKIYDKGITRSSIEGLYDALVQYRNGD
                     MYAPAIENSEALGREIKHFNHCVKHSSTPITDGKAGLRVVSILTAAQESSRNNGIRVI
                     PASVN"
     gene            1601..2287
                     /locus_tag="VMZ02_07780"
     CDS             1601..2287
                     /locus_tag="VMZ02_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HUR71870.1"
                     /translation="MTICVPAFNEEATIRQVVTDAATTLEQTSLPGEILVIDDCSTDG
                     TWKILSEMQKTVARLQLRRHETNQGIAITFAELYQWAGKGLVFLNSADGQWKMSTLLQ
                     LLPLAKRYEIVIARRPVKHYSFARQFVSWLFNAVPVAFFGIRTYDAGSVKLVHREIYD
                     IPIISHGVFGEAERIIRARRRGFRIGVRDVEHFPRISGQALGAKPRLVIEAMRDLLRC
                     WIDIIMRRRN"
     gene            2357..2836
                     /locus_tag="VMZ02_07785"
     CDS             2357..2836
                     /locus_tag="VMZ02_07785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HUR71871.1"
                     /translation="MHQNIAPDVKLGAGVKIYEFVNLYGCEIGTNTKIGTFVEIQKGA
                     RIGRNCKISSHSFICEGVQIDDEVFVGHHVVFINDRYPRATAAVGRLQTADDWQVVPT
                     LVERGASIGSGAVILCGVKIGAGAMIGAGSVVTKDVRPNTIIAGNPARLLRKSTRTR"
     gene            2833..3945
                     /locus_tag="VMZ02_07790"
     CDS             2833..3945
                     /locus_tag="VMZ02_07790"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HUR71872.1"
                     /translation="MNNRSGAIPFVDLSRQHIEIRPQIDAAIQRTLDRGTFASNQAVE
                     DFEENFAHYCATRFCVAVNSGTSALHLALKVAGVGTGDEVITTPFTFAATSWAISYVG
                     ARPVFVDVDARTCNIDAARVARAITPKTRAILPVHLYGQMADLKPIRALCDQHGLALI
                     EDAAQAHGAEDSGHRAGGYGDLGCFSFYPTKNLGAFGEAGAITTNNEKFAARLRSLRD
                     HAQTEKYRHDELGFNYRMDELQGAILNVKLAYLDGWNRARSAIAQSYTNKLAKTKLTL
                     PNESPGKKHVWHLFVVRSNQREHLCKALLEADIGRGLHYPIPLHLQPAYKFLGYNLGD
                     FPVAETVAKECLSLPLFPGLRESELDYVCQVLCGQQ"
     gene            complement(3988..>5401)
                     /locus_tag="VMZ02_07795"
     CDS             complement(3988..>5401)
                     /locus_tag="VMZ02_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71873.1"
                     /translation="LSVASIATLIVTADSGQLSIALPLIITEFGADLALVSWVALVYA
                     LITASLHLPCGRLSDLLGVGNLFLTGFILYGLSSIAAGSSQNAVQLIIFRAVQAAGSA
                     LIMANNFALVTALFPVAERGRAMGIAGGAISAIGYTMGPVIGGLLTHSFGWRSNFFVS
                     AVLAFCGFGAARLLLPAESFKPSEVKKEPFDLIGAISFACCISLILFGLTTAQKAGWQ
                     SPLVVLSLLTGIALLAFFIHWERRAGAPLLDLALFRIPAFTLGNGARWISFITMSISI
                     LLMPFYLQFALRLDPLRAGFLVAPTPLAMALLAPLTGWMSERFAAERLCAAGLTVSGV
                     AFVLMSFLSPVSAPVQIAVCLGLVGVGMGLFQTPNNNLLMTAVPRHRLGVGSAFLSIV
                     RSLGYSIGAAVATTIVGAQLAANGASSLQALTQQLAADGSAVGLAAFMRGFRFACLTA
                     AGLSFFGAAISAIRVSGEAR"
BASE COUNT         1400 a         1479 c         1429 g         1093 t
ORIGIN      
        1 atctcgggca acatgtttaa taatgatgaa tttggcgact atgttattta cgcagcatgg
       61 ccaaagtacc gtgtctttat tgacgggcga acggatatgt atggcgcctc acgggttagg
      121 gagtatttga cgattagcca agccaaggac ggttgggaag agctcctgga aaagtaccag
      181 ctaacctggg tgttccacga gcccacttca atgctgtcaa agattctcct ggagcgaacc
      241 gattggagac tgatttattc tgacaaggtc gcgaatatat ttgtaaaatc gttgccgcaa
      301 catgagcatc tgattgctaa gtacggcgcc acacagccct acaaagagac aagtagctat
      361 acaaattaaa gtcaagcaaa tataatcggc cgattgagac tgttccgaag ccctttcaca
      421 tataattcgc gcccgctaac aagcgaagga agccatactt ggacgttaat ataggaataa
      481 tcggcttggg ctactgggga cccaatcttc ttcggaattt tcatgcttcg cccgatagta
      541 atgtaatcat ttgttgcgac agccaagccg atcgatgccg gaaatttgcc gtacagtttc
      601 caaacttcac atactcgcag cttgccgaag acgtcatcaa tcaccccgaa gtgaatgccg
      661 ttgccatcgc caccccggtg cagacccatt acgagctggc gaaacaagcg ctgcttgccg
      721 ggaagcacgt cttaataacc aaaccgatga ctgacgacct cgatgaagcg agaaagttag
      781 tcgaacttgc cgataaaatg aaatgcatcc tgcaagttga ccataccttc atcttccatc
      841 cggcggttga gaaactcaaa gacatagtta ctcgcggcga actgggcgag ctctattatt
      901 tcgattcggt gcgcatcaat cttggactgt ttcaatccga tgtctcggtc atctgggatt
      961 tagcgcctca cgatgtttcg atcatggatt atcttatcga tcgaccggtg caatgggtcc
     1021 aagccatcgg cgtctcacat gccgggcaca aatccgagag tatggcatac ataacggtcc
     1081 agttcgccgg aaatgttctc ggtcattgcc atgtgaattg gttggcgccg atgaagactc
     1141 gtttagtcat gctcggaggt tcgcgaaggc tagccgtata cgacgacaat ctcgtcaccg
     1201 agaaggtcaa aatctacgac aagggcatta cccgaagcag tatcgaaggg ctttacgacg
     1261 cgctcgtgca atatcgcaat ggcgacatgt acgcaccggc tattgaaaac agcgaagcgc
     1321 taggacgcga gattaagcat ttcaatcact gtgtcaaaca ttcgagcacg cccataaccg
     1381 atgggaaagc gggactccgc gtcgtcagta ttctgacagc cgcacaggaa agcagccgca
     1441 acaacggcat acgggtcatt ccagcaagcg ttaactgagc cgcagcaacg ttttaatgca
     1501 ccagggtagc gagaattcgc agagttgcga tattgcccaa gtggacgacc gcctaccgag
     1561 acactccact cccgagtttc ctcagagcta tccaaagggt atgaccattt gcgtacccgc
     1621 gttcaacgaa gaagccacga tccgtcaggt cgtcacggac gcggccacga ctttagagca
     1681 gacatcattg ccgggggaaa ttttagtcat cgacgattgt tccaccgacg gcacttggaa
     1741 aattctctcg gaaatgcaaa aaactgtggc ccgattgcag cttcgacgcc atgaaaccaa
     1801 tcagggcatc gcaataacgt tcgccgaact ctatcagtgg gccggaaaag gactggtctt
     1861 tctcaactct gccgatggtc agtggaaaat gagtacgctc ctgcagcttt taccgttggc
     1921 gaaacgatat gaaatcgtca tcgcgcgccg gccggttaaa cactacagct tcgcccggca
     1981 attcgtatcc tggctgttta acgccgtgcc ggtggctttc tttggcatcc gcacttacga
     2041 cgccggcagt gttaaactcg tgcatcgcga gatttatgac attccaatca ttagtcacgg
     2101 cgtcttcggt gaagccgaaa gaatcatccg tgcgcgacgc aggggatttc gcattggtgt
     2161 ccgggacgtc gagcactttc cacgtatcag cggccaagca ttgggagcca agccacgcct
     2221 ggtaattgaa gccatgagag acctattacg gtgttggatc gatattatta tgcgacggcg
     2281 gaattaacgg ggtcgcggtt tgaaaatcaa aaaacctccg gtgagaagaa aaaatgcgcc
     2341 gcgcagcagc ggcgggatgc accaaaatat cgctcctgat gtgaaacttg gagccggtgt
     2401 gaaaatttat gaattcgtga atctctatgg ctgtgagatc ggtaccaaca ccaagatcgg
     2461 cacctttgtt gagattcaaa agggcgcgcg catcggccga aactgcaaaa tatcaagcca
     2521 tagttttatt tgcgaaggag tgcagatcga tgacgaggtt tttgtcggtc accatgtagt
     2581 cttcatcaac gaccgctatc caagagcaac cgcagccgtt ggcaggttgc agaccgcaga
     2641 cgactggcag gtcgtaccta cattggtcga gcgcggagct tcgatcggtt ccggggcagt
     2701 gattctctgc ggagtcaaga tcggcgctgg cgcgatgatc ggcgccggca gcgtagttac
     2761 aaaagacgtt cggcccaaca cgattatcgc cggcaacccg gcgcgccttc tgcgcaaatc
     2821 aacgaggaca cgatgaacaa tcgatctggc gccattccct ttgtcgatct aagtcgccag
     2881 cacattgaaa taaggcccca aatcgacgcg gccattcagc gcacactgga tcgagggaca
     2941 tttgcgtcga atcaagctgt ggaggatttc gaggagaact ttgcccatta ttgcgccacg
     3001 cgtttctgcg tggcggtaaa ttccggcacc agtgccctac accttgcgct aaaagtcgcc
     3061 ggggtgggca ccggcgacga agtcattacc acccctttta ctttcgcggc gacgtcatgg
     3121 gccatctcct atgtgggcgc gagaccggtt tttgtcgacg tcgatgcgcg aacttgcaac
     3181 atcgatgcag cacgagtagc acgtgccatc accccaaaga ctcgcgcaat acttcccgtc
     3241 cacctatacg gccaaatggc ggatctgaag cctattcgag cactctgcga tcaacatggc
     3301 ctggcgctga tcgaagacgc cgcacaggcc cacggagccg aagacagcgg gcatcgagcg
     3361 ggcggctatg gagatctcgg atgtttcagc ttttatccaa cgaaaaatct cggagccttc
     3421 ggggaagctg gcgcaatcac aacgaacaac gagaaatttg ccgcgcgtct gcgtagctta
     3481 cgcgatcatg ctcagaccga gaaataccgt cacgacgaac ttggatttaa ttaccgcatg
     3541 gacgagctac agggagcgat tctcaacgtt aagctcgctt atctcgacgg ctggaatcgg
     3601 gcgagatctg cgatagcgca atcgtatacg aacaaattgg ccaagaccaa actcaccctc
     3661 ccaaatgagt cgcccgggaa aaagcatgtg tggcaccttt ttgtggttcg ctctaatcag
     3721 cgggagcatc tttgtaaagc acttttggag gcggacattg gcaggggtct ccattatcct
     3781 ataccgcttc acctgcagcc ggcatacaag tttctcggtt acaatttagg tgacttcccg
     3841 gtcgccgaaa ctgtggccaa agaatgttta agcctccctc tgttccccgg tttaagggaa
     3901 tcggaacttg attacgtctg ccaggtcctt tgcgggcagc agtgacaatt ggcttcagca
     3961 ataattgtcg aatgatcgaa gcaatcatca tctggcttcg cccgacaccc gaatggcgct
     4021 gatcgccgcg ccaaaaaaac tcaaacccgc tgccgtcagg caagcgaaac ggaatccgcg
     4081 catgaacgcg gccaatccca ccgcgcttcc gtcggctgcc agttgctgcg tcaaagcttg
     4141 cagcgacgaa gcgccgttcg cggccaactg cgcaccgacg atggtcgtcg ccaccgccgc
     4201 gccgatcgaa taacccaagc tgcgcacgat cgatagaaac gccgaaccga cgccgagccg
     4261 gtggcgtggc accgcagtca tgagcagatt gttattgggc gtttggaaaa gtcccatccc
     4321 gacaccgacc agtccaaggc agacagcgat ctgaacgggt gcggacaccg gtgaaaggaa
     4381 actcatcagc acaaacgcga cgccacttac cgtcaatccg gccgcacata gccgttcggc
     4441 ggcaaacctt tccgacatcc agccggtcaa cggcgccaac agcgccattg ccaacggcgt
     4501 aggagcaacg agaaatcccg cgcgcagcgg atccaaacgg agcgcgaatt gcaaatagaa
     4561 aggcatcaac aggatgctga tgctcattgt aatgaagctg atccagcgcg cgccgttgcc
     4621 gagagtaaac gcagggatac gaaacagcgc cagatcgagc aaaggtgcgc ccgcgcgccg
     4681 ctcccaatgg atgaagaaag ccaacagcgc aatgcctgtg agcaaagaca aaaccaccag
     4741 cggcgactgc caaccggctt tctgcgccgt ggtgaggcca aacagtatta gcgaaatgca
     4801 gcaggcgaag gatatcgctc cgataaggtc gaaaggctcc tttttgactt ccgagggctt
     4861 gaaactttcc gccggcagca acaaacgcgc cgcgccgaag ccgcaaaacg cgagaactgc
     4921 ggagacaaaa aaattagagc gccagccgaa gctatgggtc aacaaaccgc cgatcaccgg
     4981 tcccatggtg taaccgatgg ccgagatcgc accgccagcg atgcccatgg cgcggccgcg
     5041 ttccgccacc gggaacaatg ccgtcaccag agcaaaatta ttggccatga tgagcgcgct
     5101 accggctgcc tgcacagcgc gaaagataat tagctgcacg gcgttctggg acgaacccgc
     5161 ggcaatcgaa ctcaatccgt aaagaatgaa tccggtcaga aacaaattgc cgacaccgag
     5221 cagatccgac agccgcccgc agggcaagtg aagcgacgcc gtgatcaagg catagacgag
     5281 tgcaacccag cttaccaatg ccagatcggc accgaactca gtgatgatca gcggcagtgc
     5341 gatgcttaac tgtccgctgt cggcagtgac aattaacgtg gcaatggaag ccaccgaaag
     5401 c
//
LOCUS       DATLCH010000264         4123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10789902, whole genome shotgun sequence.
ACCESSION   DATLCH010000264 DATLCH010000000
VERSION     DATLCH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10789902"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..538
                     /locus_tag="VMZ02_07800"
     CDS             86..538
                     /locus_tag="VMZ02_07800"
                     /inference="COORDINATES: protein motif:HMM:NF018391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HUR71874.1"
                     /translation="MVIYNLLCKKNHNFEGWFPSFEDFQKQADKKLISCPTCGTTKVE
                     KIPHACAVHVKKEQPPALPVKAKKTTPAPAAAPTAAEFKEMLLRVHHYVKENFEDVGP
                     RFAEEAKQIHKGVAEERPIHGTATLNEVKELAEEGVPCMPLPKPDLDS"
     gene            676..1758
                     /locus_tag="VMZ02_07805"
     CDS             676..1758
                     /locus_tag="VMZ02_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71875.1"
                     /translation="MLLINNKVVEQILDMKGCIEALDTGYRDLVNERANYRGRYDFFV
                     PNDDPKLMYRWGTMEGASRSFETFAIRMKSDMLEWPDGKTVEKYCVKPGTFCGFVMVF
                     STKNGEPLAIINDGIIQHMRVGACAGLGVKYLSREDSKTVGIFGSGGMARTYLLAFNE
                     VRKITKVKVYSPTKKNRETYADEMSKKLGLEIVAVDKPEEVFRGSDIVATCTDSIQVL
                     VSDPDLIESGMHLTCVKANEWSPDIVQKSDVVIRMGRPTLNLDVGQLRIGGEAAVVAG
                     NAEEIKRITSPKVDIFSQDYPVLTEIMSGKRTGRTDKNQVTFFANSGTQGLQFASTAG
                     YVVREAKKRGLGQEIPTDWLLQDIRD"
     gene            complement(1842..2483)
                     /locus_tag="VMZ02_07810"
     CDS             complement(1842..2483)
                     /locus_tag="VMZ02_07810"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HUR71876.1"
                     /translation="MSLKSIGTPRERCSACGSSLTLCMCAVLPRLAVRTKVVLVIHRH
                     ELLRSSNTGRLAVRALVNSDLRIRGESRAMLDLSDLLSSCYRSLLYYPSSDAVELTSE
                     LVAVDPRPIQLIVPDGTWRQARKIHSRHPELKGVLRVKISTPNDSTYQLRAQSRPEGM
                     ATLLAVAVALGIIEGEAVALQLMQLYQARVARTLGARGLLPRDSLWNGTSFPR"
     gene            2745..3968
                     /locus_tag="VMZ02_07815"
     CDS             2745..3968
                     /locus_tag="VMZ02_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HUR71877.1"
                     /translation="MKRSTDRILTTHTGSLPLPVQVRHALGAKRRGEPYDQSVIDTTL
                     TSAIGEVVRRQIEVGVDIVGDGELSKTAWNDYVVHRVNGLERKPRSRARSAEPWSIAQ
                     GIVRPRDSGLVRSDIENAPRHYRTDIAEFHDLARAQKDGQDNGGFAYVCTGPIGWDDF
                     AAVENDIARLGAAITTSQPHDVFMSALSPGCFVRFFRNEYYPNEESYMQAVADVMRQE
                     YQAIADAGFVLQLDCPDLASGANTDYAHLSVKQFRKVIEKHVECLNYTLKTIPREQVR
                     IHVCWGNYEGPHHRDIALSDIIDIVLKAKVTGITLESANPRHGHEWKIWQETKLPEGK
                     ILLPGVIDDISHFIEHPELVAERIVRFAKLVGKENIIACTDCGLRHLPDAGLAFAKLY
                     ALAEGARLASRELWG"
BASE COUNT          996 a         1211 c         1081 g          835 t
ORIGIN      
        1 gtaacgcggt gccgttacat tgcaattggc gccacccttg acaagccccg ctggcgatct
       61 tattgattgt gataacggta tacctatggt catctataat cttctttgta agaaaaacca
      121 caacttcgaa ggctggtttc ccagcttcga agactttcag aagcaggcgg acaagaaact
      181 catctcttgc cccacttgcg gcacgactaa agtcgaaaaa atccctcatg cttgcgctgt
      241 ccatgtaaaa aaagagcagc cgcccgcact cccggtcaag gcaaagaaaa ccacgcctgc
      301 tcctgcagcg gcgccgaccg ctgcggaatt caaagagatg ctgctgcgcg tacatcatta
      361 cgttaaagaa aatttcgagg acgtcggccc tcgcttcgcc gaggaagcca aacagattca
      421 caaaggcgtc gccgaagaac ggccgattca cggtaccgcg acgctcaacg aagtcaaaga
      481 actcgccgaa gaaggcgtac cctgcatgcc gctgcccaag cccgacctcg acagctaacc
      541 gtcacctcac tcttcatacg caacttgcaa gttgcaagca ccgccatcga gcgcgcgggc
      601 ttgtcattta tataacgctt gcaatcggaa atctggtacg aaataaaatc gtagaccgct
      661 gttagtggag gcttcatgct gctgatcaac aacaaagtcg tcgagcaaat actcgatatg
      721 aaaggctgta tcgaagccct cgacaccggc tatcgcgacc tggtaaacga acgcgccaat
      781 taccgcgggc gctacgactt cttcgttccc aacgacgacc cgaaactgat gtatcgctgg
      841 ggcaccatgg aaggcgcctc gcgctcgttc gagaccttcg ccatccgcat gaaatcggac
      901 atgctcgaat ggccggacgg caagactgtg gagaaatact gcgtcaagcc cggtacgttc
      961 tgcggcttcg tcatggtttt ttcgactaag aacggcgaac cactggcgat catcaatgac
     1021 ggcattattc aacatatgcg ggttggcgcc tgcgccggtc tcggagtgaa atatttatcc
     1081 cgtgaggatt ccaaaactgt cggcattttt ggttccggcg gcatggcacg cacctacctt
     1141 ttggccttca acgaagttcg caagatcacc aaggtaaagg tttacagccc gacgaagaag
     1201 aatcgcgaaa cctacgccga tgagatgagc aagaaactcg gcttggaaat cgttgccgtg
     1261 gacaaaccgg aagaggtttt tcgcggctcg gatatcgtcg ccacttgcac cgattcgatt
     1321 caggttctgg tcagtgatcc cgacttgatc gaatcgggca tgcatctcac ctgcgtcaaa
     1381 gccaatgagt ggagccccga catcgtgcaa aaatccgacg tagtgatccg catgggccgg
     1441 ccgacgctca atctcgacgt cgggcagctg cgaattggcg gtgaggccgc cgtggtcgcc
     1501 ggtaacgccg aagagattaa acgtatcacc agccccaagg tcgacatctt ctcccaagat
     1561 tatccggtgc tcaccgaaat catgtcaggg aaacgcaccg gtcgcaccga caaaaatcaa
     1621 gtcactttct tcgccaacag cggcacgcaa ggcctgcaat tcgcctccac ggccggctac
     1681 gtcgtgcgcg aagccaagaa acgcgggctc ggacaagaaa ttcccaccga ttggcttttg
     1741 caggatattc gcgactgacg aaaatggctc cgggtcgtcg atagtgactc attcgtcaat
     1801 cagggtgtcc gaatgattgt tcggttcgcc gtataaactt ttcaccgggg aaacgaagtc
     1861 ccattccaca gtgagtctct cggcagcagt ccgcgtgcgc caagcgtccg cgcgacgcgc
     1921 gcttgataaa gctgcatcag ctgcagcgcc accgcttcac cctcgataat ccccagcgcg
     1981 acggcaacag cgagcaatgt cgccatcccc tctggccggc tctgcgcgcg caactggtag
     2041 gtggaatcgt tgggcgtgct gatcttcact cgcagcacgc ctttaagttc ggggtgtcgc
     2101 gaatggattt tacgtgcttg gcgccaggtg ccgtcgggaa cgatcaattg aatcggacgg
     2161 gggtcgaccg caaccaattc ggatgtgagt tcgacagcgt cggagctggg atagtaaagc
     2221 aaagaccgat agcaggatga caggagatcg ctcaaatcga gcattgcccg gctttcgcca
     2281 cgaatacgca agtcgctatt gaccagcgcc ctgaccgcga gcctgcccgt gttgctgctg
     2341 cgcaacaatt cgtggcgatg gatcaccagc acaactttgg tgcgcaccgc caaacgtggc
     2401 aacaccgcgc acatacacag cgtcagtgaa gagccgcatg ccgagcaacg ctctcgtggt
     2461 gttccgatac tcttcaatga caaaattcct cgacaactta taacgtcccg gacaggcgaa
     2521 tgctatcgac aaatcggctg gctatcattg ttaatgttgt catgcccgcg agcctcggca
     2581 tccactgtct ctttcaagat gttcgccggt tttcaccggc acgatggact tttatcgtgc
     2641 ccgcagcctc tctgggcgag agcaaaaagc cccctgagac ataccgatta tttttgcttg
     2701 taaaactccc atcccgggaa taagtacaac gccaggagca ctcaatgaag cgcagcaccg
     2761 accgcattct caccacgcat accgggagcc tgccgctgcc ggtgcaggtg cgccacgcgc
     2821 tcggggccaa acgccgcggc gagccatatg atcagtccgt gatcgacaca acactgacga
     2881 gcgccattgg cgaggtagtg cggcgccaaa tcgaggtcgg cgtcgacatt gtcggcgatg
     2941 gcgagctcag taaaaccgcg tggaatgatt acgtcgtgca ccgcgtcaac ggcctggaga
     3001 gaaaaccacg ctcgcgcgcc cggagcgccg aaccttggag cattgctcag ggcatcgtcc
     3061 gcccgcgcga ctctggtctc gtgcgaagtg acatcgaaaa cgcgccgcgc cactaccgca
     3121 ccgatatcgc cgagtttcat gacctggcgc gcgcccaaaa ggatggccag gacaacggcg
     3181 gctttgccta tgtctgcacc ggtcccatcg gctgggacga ttttgccgca gtggaaaacg
     3241 acattgcccg actcggcgcg gcaatcacga catctcagcc tcatgacgtt ttcatgtcgg
     3301 ccctttcgcc cggctgcttc gtacgctttt ttagaaacga gtactatccc aacgaagaga
     3361 gctacatgca ggcggtcgcc gatgtcatgc gccaagaata ccaagccatt gccgacgccg
     3421 gcttcgtttt acaactcgat tgcccggatt tggcctccgg cgcgaatacc gactatgccc
     3481 acctgagcgt caagcaattt cgcaaagtca tcgagaaaca tgtcgagtgc ctcaactaca
     3541 cgctcaagac gatcccgcgc gaacaggtgc gcatccacgt atgctggggc aactacgaag
     3601 gaccgcacca tcgcgacatt gcgctcagcg acattatcga tatcgtgctg aaagccaaag
     3661 ttaccggcat caccctcgag tccgccaatc cacgccatgg ccatgaatgg aagatttggc
     3721 aggaaacgaa attacccgaa ggcaagatcc tcttaccggg ggtgatcgac gacatctctc
     3781 acttcattga acatcccgaa ctggtcgccg agcgcatcgt ccgcttcgcc aagctcgtcg
     3841 gcaaagaaaa tatcattgcc tgtaccgatt gtggcttgcg ccatcttccg gatgccggtc
     3901 tcgcttttgc caaactctat gcattggctg aaggcgcaag actggccagt cgagaattgt
     3961 ggggttagcg caggagtact cagctaaaga ttttgggtga tatgtgcggg cgtctcaacc
     4021 aagcctgctc ttttcgatcg caatttcgaa taagcaatgc ataacgtcaa aacaatattt
     4081 aggagaacat gccgatggca aagacaacac tcaatcccga tgg
//
LOCUS       DATLCH010000265         3142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5171699, whole genome shotgun sequence.
ACCESSION   DATLCH010000265 DATLCH010000000
VERSION     DATLCH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5171699"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="dxs"
                     /locus_tag="VMZ02_07820"
     CDS             <1..1188
                     /gene="dxs"
                     /locus_tag="VMZ02_07820"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HUR71878.1"
                     /translation="GFQYVGPIDGHDIAAMVATLENIKKVEGPTLVHVLTKKGKGFAL
                     AEKDPIKFHGVTPFHVLTGKAKKDKSPVPSYTDIFGRALVRLATDNPKIVGITAAMGS
                     GTGIDKLQREFPQRTYDVGIAEQHAVTFAAGMATEGYIPVVAIYSTFLQRGYDEILHD
                     VCLQNLHVVLALDRGGLVGADGPTHHGVFDFAYMRSVPNMVIMAPKDENELQHMLKTA
                     VEHAGPISLRYPRGEGWNVALDDEFHSLEIGKAELLRDGTDLVIAAIGHTVIPALRAA
                     QDLVSLGINAAVVNARFVKPLDSNLFRDLLIKVPRLITVEDHVISGGFGSALVEFLAD
                     EGITGVEVKRLGVPDRFIPHGTQDELKKICGFDKDAIAQAALQMMRRGKKKTRDGWER
                     GSA"
     gene            1167..1916
                     /locus_tag="VMZ02_07825"
     CDS             1167..1916
                     /locus_tag="VMZ02_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HUR71879.1"
                     /translation="MGKRQRLDLLLVERQLVASREDGRRRILAGEILVNQQPVTKAGN
                     LIDAAATISVKAAPRYVSRGGVKLEKALREFTIQVAGKSCIDIGASTGGFTDCLLAHG
                     AGKVIAVDVGYGQLDWQLRNDPRVLVLERTNIRYLTPANLPFVPELATIDASFISLKL
                     ILPTVRLLIAADATVIALIKPQFEVGKGKVGKGGVVRSAEEHNRVVAEISQASIDCGY
                     RVLGVTQSPLLGPQGNKEFLLYLKAGVALSA"
     gene            1947..2678
                     /locus_tag="VMZ02_07830"
     CDS             1947..2678
                     /locus_tag="VMZ02_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HUR71880.1"
                     /translation="MFPLKKIAHRGASGNYPENSRLAFEKAIAAGADMIELDCRLSVD
                     GHVVVFHDEKLARTTGARGKMQDKTLEQLRKLDIGKWHGPAFRGERILTLEEALEILH
                     GAVDLCLEIKTTAAVPAGIELKLLFILSHYDYLERTTISSFEYRSLARVRELAPEATL
                     GVLYGSGIHEDPFAAAERLNASSIHVQKQLARREFLDQAWQAGLDIYVWTVNDLREME
                     MFAALGVQGIITDYPEKLVRLGRGR"
     gene            2860..3054
                     /gene="rpsU"
                     /locus_tag="VMZ02_07835"
     CDS             2860..3054
                     /gene="rpsU"
                     /locus_tag="VMZ02_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HUR71881.1"
                     /translation="MTGVRVRDNESIESAIRRFKKLCEKAGILAELRKREHYEKPSVK
                     RKKKAIAAKKRALRRVRHSF"
BASE COUNT          710 a          799 c          920 g          713 t
ORIGIN      
        1 ggattccagt atgtcggccc gatcgatgga catgatatcg ccgccatggt cgccacgttg
       61 gagaatatca agaaagtcga agggccgacg ttggtgcacg tcctgaccaa gaagggcaag
      121 ggttttgcgc tcgccgaaaa agatccgata aagtttcacg gtgtgacgcc atttcacgtg
      181 ctgacgggca aagcgaaaaa agacaaaagc ccggtgccga gttacaccga tatatttggc
      241 cgggctctgg ttcgtctcgc cacagacaat cccaagatcg tcggtattac tgcggccatg
      301 ggcagcggca ctggcatcga caagctgcag cgcgagttcc cccagcgaac ctacgatgtc
      361 ggcatcgctg aacagcacgc cgtcactttc gccgccggta tggcgaccga gggttacatt
      421 cctgtggtgg cgatctattc gacttttttg caacgtggtt acgacgagat cctacacgat
      481 gtctgtttgc agaaccttca tgtggtgctg gcgctcgatc gcggcggctt ggtcggcgcc
      541 gacggtccga cgcaccatgg cgtctttgac tttgcttaca tgcgctcagt ccccaatatg
      601 gtcatcatgg cacccaagga cgagaatgaa ttgcagcata tgctcaagac cgcggtggag
      661 catgccgggc cgatatcgtt acgctatccg cgcggcgaag gttggaacgt agcgcttgat
      721 gatgaatttc atagccttga gatcggcaag gcagagctgt tgcgcgacgg caccgatcta
      781 gtcatagccg cgattggtca cactgtgata ccggcgctta gagcagccca agacttggta
      841 tccctcggta ttaatgcggc ggttgtcaat gcgcgtttcg tcaagccatt ggatagcaat
      901 ctgtttcgcg atctgttgat caaggtgccg cgtctcatca ccgtcgagga ccacgtgatc
      961 agtggcgggt ttggcagcgc gttggtggaa tttttggcgg acgaaggcat caccggagtg
     1021 gaagtcaaac gcctcggtgt acccgatcgt tttattcccc acggcacaca agacgagctc
     1081 aaaaagatct gtggcttcga taaagatgcc atcgcgcagg cggccttgca gatgatgcgc
     1141 cggggcaaaa agaaaactag agacggatgg gaaagaggca gcgcttagat ctgctcttgg
     1201 tcgagcgcca gcttgtggcg agccgggaag atgggcggcg gcgcattctc gccggtgaga
     1261 ttcttgtcaa ccagcagccg gtcaccaaag cgggcaacct gattgacgcg gcagcgacga
     1321 tcagcgtaaa agccgcgccc cgctatgtca gccgcggcgg tgtcaagctg gagaaagcgc
     1381 tgcgcgagtt cacgatccaa gttgccggta aaagttgcat cgatattggc gcctccactg
     1441 gaggttttac cgactgtctg ctcgctcatg gagctggcaa ggtgatcgct gtcgatgtcg
     1501 gttatggtca actcgactgg cagcttcgca acgatccccg ggtcttggtt ctcgaacgaa
     1561 ccaacattcg atacctcact ccagcgaatc tgccgtttgt tccggaactc gcaactatcg
     1621 acgcatcgtt tatatcgctg aaattgattc tgcccacggt tcgcctgctc attgctgccg
     1681 atgccactgt gattgcgctc attaaaccgc aattcgaagt cggcaaggga aaggtcggta
     1741 aaggtggagt cgtgcgctcg gccgaagagc ataaccgagt cgtcgcggaa atatcgcaag
     1801 catcgatcga ctgcggttat cgagtgcttg gggtgaccca atcgccgctt ttgggccccc
     1861 agggtaataa ggagttcctg ctctatctca aagctggagt cgcgctgtcg gcgtagcgtt
     1921 tgtgcggttc gcgggactct ctcctaatgt ttcctcttaa gaaaatcgcc catcggggtg
     1981 cctcagggaa ctatccggag aatagccgat tggctttcga aaaagccatt gcggcgggcg
     2041 cggatatgat cgagttggac tgccgacttt cggtcgatgg ccacgttgtg gtgtttcacg
     2101 atgaaaagct cgcgcggacc actggcgcgc gcggtaaaat gcaagataag acgctggagc
     2161 agttgcgtaa gctcgacatc ggtaagtggc atgggccggc ctttcgtggc gagagaattt
     2221 tgaccttgga agaggcgctg gaaattttgc acggcgctgt tgatctctgc ttggaaataa
     2281 aaaccaccgc tgctgtgccc gccgggattg aattgaagct cctgtttatt ttgagccact
     2341 acgattattt ggaacgcacc acgatctcgt ccttcgagta ccgcagtctg gcgcgggtgc
     2401 gcgagttggc gcctgaggcc acacttgggg tgctctatgg gtctggcatt cacgaagatc
     2461 cgttcgccgc cgcagaacgg ctgaacgcat cttcgatcca tgtccaaaaa caattggcgc
     2521 ggcgcgagtt tctcgatcag gcatggcaag ctggcctgga tatttatgtc tggaccgtca
     2581 acgatctgcg cgagatggaa atgttcgccg cgctaggcgt acagggaatc ataactgatt
     2641 atccggaaaa gctcgtgcga cttggccgag gtcgttaatc acgaatgctc gtggcgcctc
     2701 ggctttgccg tcgaagtcgc gcgcgtgtcg ccgtatttgg tcttcgcggg actgcaaacg
     2761 gttagattta aaagtgattt gcttgacttg aaaaaattat cagctaaaat cagatccgat
     2821 ttatgtgcgg tctgcctgga tcgggggtga gtcatttcta tgaccggagt tcgtgttcga
     2881 gataacgaat cgattgaaag tgccatccgg cgttttaaaa aattatgcga aaaagccggc
     2941 atcctcgccg aattgcgtaa acgagaacac tacgagaagc ccagcgtaaa acgtaagaaa
     3001 aaagcgattg ccgcgaaaaa acgcgctctg cgacgcgttc gtcattcatt ctaagattta
     3061 attgagatct atataccgat ggatttgaaa gcggagattc aacaggccgt gaaagccgcc
     3121 atgaaaggcg gtgacacggt ca
//
LOCUS       DATLCH010000266         3987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7064838, whole genome shotgun sequence.
ACCESSION   DATLCH010000266 DATLCH010000000
VERSION     DATLCH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7064838"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VMZ02_07840"
     CDS             complement(<1..408)
                     /locus_tag="VMZ02_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HUR71882.1"
                     /translation="MDPIRLRARALRHNATDTEKLLWRRIRMWQVDDYKFRRQQPLGR
                     YIVDFVCLEKRVVVELDGAQHAEQSANDMRRDEWLREQGFIVLRFWNHEVVQNIDDVT
                     EKIFQTVKSTPFLSPSPQGGRKKIGGRVLRYKLR"
     gene            complement(517..708)
                     /locus_tag="VMZ02_07845"
     CDS             complement(517..708)
                     /locus_tag="VMZ02_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71883.1"
                     /translation="MELARTEAKFFGIAKLPLVEVPHHPSGGAREGEHRADAEGAIGL
                     VDELLGLRAAGGLKQAKAG"
     gene            complement(796..1083)
                     /locus_tag="VMZ02_07850"
     CDS             complement(796..1083)
                     /locus_tag="VMZ02_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71884.1"
                     /translation="MATIKFLDPRAAVNLKERPLIPGLESLEGKVIGIIDNGQSNSTD
                     MFKELAELLQERLHVKEVLFKTKPTHMQGAPKPIMEEILNRCDAVITGLGA"
     gene            1290..2201
                     /locus_tag="VMZ02_07855"
     CDS             1290..2201
                     /locus_tag="VMZ02_07855"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71885.1"
                     /translation="MSLLFSSPLGAQVATLRVGTNSPASAESVLFSIARDAGVLKQSQ
                     LDVEVIFIAGGTLSMQALIGKSLDFLCTGGTPYIVAYLEGAQTKMIGGVNNKLPYGFV
                     SLPNIKTPAQLKGKRIGISRFGSTDDFAVKLALSQFGLNPKTDVSITQVGSAATRLTA
                     LQAGSIDAAVLTLGLVQIGQRSGLLLLDFIEKDIEYQQVAIIARDDLLKSRADLVRRF
                     MKGYLDSVRFYKTQKEIAIRETMKRLRTSDRAIAEFDYVQRVRSLPDDGKPSIKGMQL
                     ALDDIAKDNPKAKSLTVQQLIDLSFLP"
     gene            2297..3394
                     /locus_tag="VMZ02_07860"
     CDS             2297..3394
                     /locus_tag="VMZ02_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71886.1"
                     /translation="MQPIIDADTHISESEEMWAMFDQEMFGRRPVLLSLPDDTLYGKW
                     NKTWLIDGNIFPKPAGKGGFRLITPSASTIQSNRKDLSVACRDMTDISGRLADMDRLG
                     IAAQIVYPTLFLVYLTDDPELDVALSRAYNRFIAQACAKSNGRIHWTAIPPLRDIEES
                     VKELNWAKQNGAVGVFFRGMEGNYTLDNPRFFPVYQAASDLDLPICVHTGSSTPVISE
                     MFDLERNRQWSHSAFPPLHAFRDLVLNQIPDMFPKLRFGFLEASASWIPFLIHKLKRD
                     NTKRWKPSWKSPRDLFEDCRFYVACEADEDLNYLMEYAGIDHLISGSDYGHNDPAEQA
                     QLVKQMQSREDVAPAVIEKILCDNPRQFYKI"
     gene            3526..>3987
                     /locus_tag="VMZ02_07865"
     CDS             3526..>3987
                     /locus_tag="VMZ02_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71887.1"
                     /translation="MKNDSRAPLRHISVVIACLSALLGSLDSAINIGFPAITAAFSLE
                     VTAIQWVIVGYVLTHASLLIGCGALADRWGHGRMLAYGLLVSAVGFTCCGVAPTFTWL
                     VAGRVIQGGGAALVFATAPALVTLAVSSESRGRALGIYQMSNAVGYAVGPLL"
BASE COUNT          844 a         1197 c         1032 g          914 t
ORIGIN      
        1 gcgcagttta taccggagta ccctcccccc gatcttcttc cttccccctt gcggggaagg
       61 actgaggaag ggggtgcttt tcacagtttg gaaaatcttt tcagtgacgt cgtcaatgtt
      121 ttgcacgact tcgtgattcc aaaatctaag aacaatgaat ccttgctcgc gcaaccattc
      181 atcccttcgc atatcgttgg cgctttgttc cgcgtgttgc gccccgtcga gttcgacaac
      241 gactcgcttc tccaagcaga caaagtcgac gatgtagcgt cccagcggtt gttggcggcg
      301 gaatttatag tcgtcaacct gccacattct gattcgacgc caaagcaact tctcggtgtc
      361 cgtggcatta tggcggagtg ccctggcgcg caacctaatt ggatccattc cccctatcct
      421 aacctttccc cgcaaggggg aaaggaatcg caatgcctac taacacaact aagcgtgatg
      481 ccccaggtcg gctctaaaat cagcgacgca cattggctat ccagccttcg cctgcttcag
      541 ccctcccgcc gcacgcaaac ctagcagctc atcaaccaat ccaatcgccc cttccgcgtc
      601 cgctcgatgt tccccttcgc gcgcgccgcc gcttgggtgg tgcgggactt cgactagcgg
      661 gagtttggct atgccgaaga atttcgcttc ggtgcgcgcc agctccatga agatcgactg
      721 gcacagcgtg accgtcggct tgcctttctt ttgtaattcg atggcgtcgt ggacactcca
      781 cgacgtgcac gaccctcaag cgcccaagcc ggtgatgacc gcatcacacc ggttaagtat
      841 ctcctccatg atcggtttgg gcgcaccctg catatgcgtc ggcttggttt tgaacagcac
      901 ttccttcacg tggagccgtt cctggagcag ctcggccaat tctttgaaca tgtccgtcga
      961 attggactgg ccattgtcga tgatgccgat aaccttgcct tcgagggact ccaagcccgg
     1021 tatcaacggt cgctctttga gattgaccgc tgcgcgcgga tcgagaaatt tgatagtcgc
     1081 catcgatttt ttacctcctg aacaatcctg ccttgtctca gcaccttata taggcaaact
     1141 cgcgatgggc ctgcaaccac cgataacgcc tttcacgttg acagccggat tggtttgcat
     1201 tagctcttac gacggcgatg gagaatccag taattccatt ttttgcagta ttgcttcaac
     1261 gctgctctgt ccggctcgcg ctagcgatag tgagcctgtt attcagcagc ccactcgggg
     1321 cgcaagtggc gaccctgcgc gtcggcacca actcaccggc aagcgccgag tcggtgctgt
     1381 tcagcatcgc cagggacgcc ggcgtgctga agcagagcca gctcgacgtc gaagtaattt
     1441 ttatcgccgg cggcacactg tcgatgcagg cgctgatcgg caaaagcttg gattttctct
     1501 gcaccggcgg gactccgtat atagtcgcct atctcgaagg cgcccaaact aaaatgatcg
     1561 gcggtgtcaa caacaagctg ccctacggtt tcgtttcgct gcccaacatc aagacgccgg
     1621 cccagctcaa aggtaaacgc atcggcatca gccgtttcgg cagcaccgat gatttcgccg
     1681 taaagctggc gctgtcgcag ttcggtttga atccgaaaac agatgttagc atcacccagg
     1741 ttggcagcgc cgccactcgt ttgaccgcac ttcaagccgg cagcatcgac gccgccgtgc
     1801 tcactctcgg cctagtgcaa atcggccagc ggagcggtct cctgctgctc gatttcatcg
     1861 agaaagacat cgagtaccag caagtcgcga tcatcgcccg cgatgatctg ctcaagagcc
     1921 gcgccgatct ggtgcgccgc tttatgaaag gttatctcga cagcgtgcgt ttttataaaa
     1981 cgcaaaaaga aattgccatc cgcgaaacca tgaaacgcct gcgcaccagc gatcgcgcca
     2041 tcgcggaatt cgactatgtc caacgggtgc ggtctttacc cgacgatggc aagcccagca
     2101 tcaaaggcat gcagctcgcg ctagacgata tcgccaagga caacccgaaa gcgaaaagct
     2161 tgaccgtgca acaactgatc gatttgagtt tcttgccgta gacatttcgt ttaaagtcca
     2221 gagtcaggcg tctcgtttgt cgaatgtaac gccagccgcg agcaattaac tgtcgaccaa
     2281 agcaccggag gtctttatgc aaccgattat cgacgctgat acgcatattt ccgaatccga
     2341 agagatgtgg gcgatgttcg atcaagaaat gttcggtcgc cgccccgtgc tgctttcgct
     2401 gccggacgac acgctatacg gcaagtggaa caaaacttgg ctaatcgacg gcaatatttt
     2461 ccccaagcct gcgggcaaag gcggttttcg cttgatcacg ccgtctgcct cgacgatcca
     2521 gtccaatcgc aaagacttga gcgtcgcgtg ccgcgacatg accgacattt ccggccggct
     2581 ggccgacatg gaccgcctcg gcattgccgc tcaaatcgtc tacccgacgc tgtttctggt
     2641 ttacttgaca gacgaccccg aactcgacgt cgcgctctcc cgggcgtaca accgcttcat
     2701 cgcccaagcc tgcgccaaga gtaatggccg tatccattgg actgccattc cgccgctgcg
     2761 cgacatcgaa gaatcggtta aggaattgaa ttgggccaag caaaacggcg cagtcggcgt
     2821 gtttttccgc ggcatggaag gcaactacac gctcgacaat ccgcggttct ttccggtgta
     2881 ccaagccgcc agcgatcttg atttgccgat ctgcgtccat accggctcga gcacgccggt
     2941 gatcagcgag atgttcgacc tggagcgcaa ccgccaatgg tcgcattcgg catttccccc
     3001 gttgcacgcg ttccgcgatc tagtgctcaa ccaaatcccc gacatgtttc ccaaactgcg
     3061 cttcggtttt ctcgaagcat cggcgagttg gattcccttc ctcattcata aactcaaaag
     3121 ggacaatacc aaacgctgga aaccatcgtg gaagtcgcca cgcgacttgt tcgaagactg
     3181 ccgcttctac gtcgcctgcg aagccgacga agatttgaat tatttgatgg aatacgccgg
     3241 tatcgatcac ctgatcagcg gctcggacta tggccacaac gatcccgccg agcaggccca
     3301 gctggttaaa caaatgcagt cgcgcgaaga tgtcgcgccg gcagtgatcg aaaaaattct
     3361 ctgcgataat ccgcggcagt tctataagat ttgagattaa gccttcggcg attacatcgc
     3421 gaactctgca gagcacgcgg aactagaata ctttactttc ttcctcggtg atctccgcgc
     3481 ctctacggtg agtaatccga atcgagaccg gcaccggaat ctttcttgaa aaatgacagt
     3541 cgcgctccgc tccggcacat ttcagtcgtc attgcctgtc tcagcgcgtt gttaggttca
     3601 ctcgacagcg ccatcaacat cggctttccg gcgatcaccg cagcattttc tctcgaggtc
     3661 acagcgatcc aatgggtcat cgtcggctat gtcctgaccc atgcaagcct gttgataggc
     3721 tgtggtgccc tggcggaccg ctggggtcat ggccgcatgt tggcttacgg attgttagta
     3781 agcgccgtag ggttcacctg ctgcggcgtt gcgccgacct ttacctggct cgtcgccgga
     3841 cgagtcatcc agggcggagg cgccgcgctt gttttcgcca ccgctcccgc attagtgacc
     3901 ttggcggtca gcagtgaaag tcgcggtcgc gcgctgggaa tttatcaaat gtctaacgcc
     3961 gtcggttatg ccgtcggtcc gttgctc
//
LOCUS       DATLCH010000267         6649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2944809, whole genome shotgun sequence.
ACCESSION   DATLCH010000267 DATLCH010000000
VERSION     DATLCH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944809"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VMZ02_07870"
     CDS             <1..274
                     /locus_tag="VMZ02_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71888.1"
                     /translation="QGVDWGVVSGLVNNRVVISVRNVGYVKSAGEIMKKLYDDIGSAG
                     GHRAMAKAVVPLDRFKDRFGEVSEKVIREAMLPLIIDRDQIELTLR"
     gene            437..793
                     /locus_tag="VMZ02_07875"
     CDS             437..793
                     /locus_tag="VMZ02_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR71889.1"
                     /translation="MECINLEQKIDNARAQKIKNNHFFGTDNFRSWMLYFEPGDDTPM
                     HFHQNSETFLVVQGECAIKDINGGERVVKKNDIVFFAAKEYYQLVNKGSEPLVLYGNR
                     AEPFGIGITRAETAKK"
     gene            819..1211
                     /locus_tag="VMZ02_07880"
     CDS             819..1211
                     /locus_tag="VMZ02_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71890.1"
                     /translation="MPKIRHLAIRTEDTGKLATFYMDVFEMQMLHKDKQEGGAIYLTD
                     GYFNLAILPNHEQQSPNGLYHFGFEVENGEKIVERLQKVNPNKLPKQRPNGRPYAETR
                     CSDIDGNYFDISEHGFLNVEPVGEKKPE"
     gene            1222..2244
                     /locus_tag="VMZ02_07885"
     CDS             1222..2244
                     /locus_tag="VMZ02_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71891.1"
                     /translation="MIRRGHNNRHYQSIVALAVAVLFFALPAAAQIKSNFASSVTSES
                     MTPMWVARERGLFKKHGLDMQYVVIPRSPLAVAALVAGEIDAAIIGPGHLINSASTGS
                     DLIGIANFFQKLDYRLVARPEIKKLDDFRGKRVAISGPGATSHLVTMLAFQGMNLDPV
                     QAKIILLTIPGTELNRRLAMESGAIEATTLRGAIGELYGHKGYNVLYNLSAMGVTLPQ
                     SVLVTSRRTATAKPQVVDAYLRAMLEAIAVTIDPANKSSVMRAIMTNLRLSNPADAEE
                     AYNAVVNSIERVPYSHLDSMKRLHQLLMQINPKVADVRVETVIENSFINKLESSGYIQ
                     SVYKKN"
     gene            2268..3734
                     /gene="tcuA"
                     /locus_tag="VMZ02_07890"
     CDS             2268..3734
                     /gene="tcuA"
                     /locus_tag="VMZ02_07890"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HUR71892.1"
                     /translation="MRTENIPVVVVGGGSAAFEAAVAAKQAGAPKVVMLEKAPEPEFG
                     GNARYSHTGFRWVFSGADEIRQFLPEVDDALFEKLNLPAYSSELFHADLQRVTRGRID
                     QTLADVLVSQSNAAIHWMRELGIKWEIDSHVVIDGRYYFEPGLVIHPVGGVGGGLGQL
                     GQWREIALRLGVDLRYESRVKALLGNDRHIEGIVVSDPIEEYEIKSSSVILCAGGFQA
                     NPEMRARYLGANADLMKVRGSRHDTGEVLMMTLAMGAKAAGHWQGAHATPIDSTYPDV
                     EIGSKANRYGYPYSITVNTLGQRFFDEGEARHSYTYAKTGWAVLGQPGALAYQIYDQK
                     TIPLLGTRYEFSTPIEAQSIEELAGKIGIEPTVLKHTVTEFNNAARKDVVFDATKLDG
                     KCTEGITPKKSNWANPIDKPPYWAFSITGGVTFTFGGLQINTQAQVLNTSGNPIRGLF
                     ASGDIVGLFFHNYPSCTGQTRNVVFSRLAGRNAAAQGL"
     gene            complement(3838..4302)
                     /locus_tag="VMZ02_07895"
     CDS             complement(3838..4302)
                     /locus_tag="VMZ02_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HUR71893.1"
                     /translation="MFNELMRWNPSEELSNWHRDIDDLFGRFFGRTEAPAGGWMPRLE
                     TCRKDNNYVVRIDLPGVDPRNLSVQAEGNVLTISGERKSADTHAEYRETSYGKFERAL
                     TLPQGVETDKIAAHYENGVLEVTVPLPPQLAGRKIPVQIEQKENKKIESKAA"
     gene            complement(4396..5232)
                     /locus_tag="VMZ02_07900"
     CDS             complement(4396..5232)
                     /locus_tag="VMZ02_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HUR71894.1"
                     /translation="MRRYLVLANQTASSPELTSAVQKLVQKDKDAEFVLLVPATPTEE
                     LLDWQEGDSAAVAKQTAQAAKEQLERAGAKVIRSEVGDPSPVKAIEDELQRHKETYDS
                     IVIATLPLQRSRWVALDQPRRIERRYKLPVIHVVGHSVTMSREELINGLNEDLNLELE
                     AILRSVYHAAAGRGMLGHELREMIKKELPSELDHATFLADKIVALGGEVRIRPNAPGE
                     LTAPRQLLDENIAAERKIITNYAKRIEQAAEFGDKGLVIRLEDILAAETDHLEALERL
                     GR"
     gene            complement(5394..6005)
                     /locus_tag="VMZ02_07905"
     CDS             complement(5394..6005)
                     /locus_tag="VMZ02_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HUR71895.1"
                     /translation="MLRLCSKSLLAIALVVTMFSNAHADTLYSEAVNGELSDSGLSPT
                     EITVAVGSNIIRGTTGGSTAPNTRDYFSIVVPSDLLLVSLIELAGTQAGNVGFLGLQS
                     GPQVTLLTNTTTAAGLLGWIHYGPTATDINILPTMGIPANGSSGFAPPLGPGSYTFWL
                     QDSSPGTFQYGFDLVLKVPEPSAAALMFAGLFALWTVLRRRPD"
     gene            6271..>6649
                     /locus_tag="VMZ02_07910"
     CDS             6271..>6649
                     /locus_tag="VMZ02_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71896.1"
                     /translation="MNSQTSKLVIDSDAHVVECAHTWDFMDKSEEKYRPIPLEAREEA
                     GVRQQFWLVDGKVRGFRFAVLSDELMAQRQQQAGRKFAEQAESRELANVDLRLEHMDQ
                     TQVDIQVLHNTMFIESCTDRPAVD"
BASE COUNT         1490 a         1850 c         1860 g         1449 t
ORIGIN      
        1 ccaaggcgtc gactggggtg tggtctcggg tttggtgaat aatcgcgtcg tcatctccgt
       61 gcgcaatgtc gggtatgtga aaagcgccgg cgaaatcatg aaaaaacttt acgacgacat
      121 tggcagtgcc ggcggtcacc gcgccatggc caaagccgtc gtgccactcg accgtttcaa
      181 agatcgtttt ggcgaagtca gcgagaaagt tatccgtgag gcgatgctgc cgctgatcat
      241 cgatcgcgat cagatcgagc ttacgctgcg ctaaaaattt tttacgtccg tatggcaatt
      301 gtttacgtag agttcccaca atcgctatcc tgccgagctt cactgcttcg aatcagcgtt
      361 tcctcaaaca attgacaagc cgacccgcac cgcgataggt tcaaccgagt tgacggcggg
      421 agttgcggag ggcgttatgg aatgcatcaa tttggaacaa aagatcgaca atgcgcgggc
      481 gcagaaaatc aagaacaacc attttttcgg caccgacaat ttccgctcgt ggatgcttta
      541 cttcgaaccg ggcgatgaca cgccgatgca ctttcatcag aactcggaaa catttttggt
      601 cgttcaaggc gaatgtgcga ttaaagatat caacggcggt gagcgcgtcg tgaaaaagaa
      661 cgacatcgtg ttctttgccg ccaaggaata ttatcaacta gtcaacaaag gcagcgagcc
      721 gttggtgctc tacggcaatc gggccgaacc gtttggcatc ggaattacac gagcggaaac
      781 agcaaaaaag tgagctaaaa ccaatccagg aggatactat gcctaagatt cgtcatctcg
      841 caatccgtac cgaagatacc ggcaaactcg cgacctttta catggacgtt tttgaaatgc
      901 agatgctgca caaggacaag caggaaggcg gcgcgattta tctgaccgat ggttatttca
      961 atctggcaat cctgcccaac catgaacagc aatcgcccaa tgggctttac cacttcggct
     1021 tcgaagtgga gaacggcgag aagatcgtcg agcggttaca gaaagtcaat cccaataagc
     1081 tccccaaaca gcgccccaac ggccgcccct acgcggaaac ccgctgttcg gatattgacg
     1141 gcaactattt cgatatttcc gagcatggct ttctgaacgt cgagcccgtg ggcgagaaga
     1201 agcccgaata attcgccaaa aatgattcgt cgcggtcata acaaccgcca ttaccaatcg
     1261 atcgtagcat tggcagtggc ggttttgttt tttgcgctgc cggcagcggc gcagatcaag
     1321 tccaatttcg cctcgagtgt cacgagcgag agcatgacgc cgatgtgggt ggcgcgcgag
     1381 cgcggccttt ttaaaaagca tggtctcgac atgcagtacg tcgtcatccc gcgctcgccg
     1441 ctggcggtgg ccgcgctggt cgccggtgaa atagatgcag ccatcatcgg tccgggtcac
     1501 ctgatcaact cggccagcac cggttccgac ctcatcggca tcgccaattt ttttcagaaa
     1561 cttgattatc gcttagtggc tcgccccgag atcaagaagc tcgacgattt ccgcggcaaa
     1621 cgggtggcga tcagtggccc gggcgcaact tcgcatctgg ttaccatgct ggcgtttcag
     1681 ggcatgaatt tggatccggt gcaggccaaa atcatactgc tgacgatccc cggcaccgaa
     1741 ctcaatcggc gcttggcgat ggagagcggc gccatcgaag cgaccactct gcgcggcgcg
     1801 atcggcgaac tgtacggcca taaaggttac aacgtgcttt acaacttgag tgcgatgggt
     1861 gtgactttgc cgcaaagcgt gttggtgacc agccggcgca ccgcgaccgc caaaccgcag
     1921 gtcgtcgatg cctatctccg ggccatgcta gaggcgatcg cggtcaccat cgatccggca
     1981 aataaaagca gcgtgatgcg tgcgatcatg accaatttgc gtttgtccaa ccctgccgat
     2041 gccgaagaag cttacaacgc cgtggttaat tccatcgagc gcgtgcccta ttcgcatttg
     2101 gatagcatga aacggctgca tcagttgctc atgcagatca atcccaaggt cgccgatgtc
     2161 cgggttgaaa cggtgatcga aaattcattt atcaataagt tggaaagttc cggttatatt
     2221 cagagtgtgt acaagaagaa ttaacttgcg gcggaaggga ccagagcgtg agaactgaaa
     2281 acattccagt cgtagtggtc ggcggcggca gcgccgcatt cgaagccgcg gtggcggcca
     2341 agcaggccgg cgcgccgaaa gtcgtcatgc tcgaaaaagc gccggagccg gagtttggcg
     2401 gcaacgcgcg ctattcccac accggtttcc gctgggtgtt ttccggcgcg gatgagatcc
     2461 gccagttttt gccggaggtc gacgacgcgc tgtttgagaa acttaacttg ccggcctatt
     2521 cgagcgagct gtttcacgcc gacctgcagc gagtcacgcg cggccgcatc gaccagacct
     2581 tggcggacgt gctggtgagc cagtccaacg cggcgattca ctggatgcgc gaattgggca
     2641 tcaagtggga aatcgatagc catgtcgtca tcgacggccg ttattatttc gaaccgggat
     2701 tggtgatcca tccagtcggc ggcgtcggcg gcggcctcgg ccagctcgga caatggcgcg
     2761 aaatcgccct gcgcctgggc gtcgacctgc gctacgaatc gcgggtaaaa gcgttgctcg
     2821 gcaacgaccg ccatatcgaa ggcatcgtgg tctccgatcc cattgaggaa tacgaaatca
     2881 aaagcagctc ggtcattctc tgcgctggcg gttttcaggc caatccggaa atgcgcgcgc
     2941 gttatctcgg agccaatgcc gatctgatga aagtgcgcgg cagccggcac gataccggcg
     3001 aagttttgat gatgacctta gcgatgggcg ccaaggccgc cggccactgg cagggcgccc
     3061 acgcgacgcc gatcgattcg acttatcccg acgtcgagat cggcagcaaa gccaaccgct
     3121 acggctatcc ctacagcatc acggtcaata ccttggggca gcgctttttc gacgaaggcg
     3181 aagcgcgcca ctcgtacacc tacgcgaaaa ccggctgggc ggtactggga cagcccggtg
     3241 cgctggcgta ccagatctac gatcagaaga cgattccgct gctcggcacg cgttacgaat
     3301 tttcgacacc tattgaagcg caatcgatag aagagctggc cggcaagatc ggcatcgagc
     3361 ccaccgtgct caaacatacc gtgacggaat tcaataatgc ggcgcgcaag gacgtcgtct
     3421 tcgatgcaac taaactcgac ggcaaatgca ccgaaggcat cacgccgaaa aaatcaaatt
     3481 gggcgaatcc gatagataag ccgccttact gggcgttttc gatcaccggc ggcgtgactt
     3541 tcactttcgg cggattgcag atcaacaccc aagcccaggt gttaaacact tccggcaatc
     3601 cgatccgcgg cctgttcgcc tcgggcgaca tcgtcgggct gttctttcac aattatcctt
     3661 cgtgcaccgg acagacccgc aacgtagtgt tctcgcgctt ggcgggccgg aatgcggcgg
     3721 cgcaggggct ttagtttgcg gtctgggcga cggctaacca atctcatgcc agcaaaaaaa
     3781 caacggggcc gtgcatgcgc ggccccgttt gaaccaacct tcgacgagcc gtgaaagtta
     3841 cgcggctttc gattcgattt tcttgttttc cttttgctcg atttgcaccg ggatcttccg
     3901 gccggcgagc tgtggcggga gcggtacagt gacttcgagc acaccgtttt cgtagtgcgc
     3961 ggcgatcttg tcggtctcca cgccttgcgg cagggtcagc gcgcgctcga acttgccata
     4021 ggaggtctcg cggtattcgg catgggtgtc cgccgattta cgttcacccg aaatcgtcag
     4081 cacattgcct tcggcctgga ccgaaaggtt tctcggatcg acgcccggca gatcgatgcg
     4141 gacgacgtag ttattatcct tacggcaggt ttccaaacgc ggcatccacc cgcccgcggg
     4201 agcctcagtg cgtccgaaga accgaccgaa cagatcgtcg atgtcgcggt gccaattgga
     4261 caattcttcc gacggattcc atctcatcaa ttcattgaac ataactgttt ctcctttcgc
     4321 ttaataataa tttctgaaaa gaatttaagc agctcggtgc atctgtcaag cccgggcggc
     4381 catcggcggc cagcgctagc ggcctaatcg ttccagcgct tcgaggtggt cggtttccgc
     4441 agccagtata tcttccaacc gtatgaccaa gcctttgtcg ccgaattccg ctgcctgttc
     4501 gatgcgcttg gcatagttgg ttatgatttt ccgttcggct gcgatgttct cgtctaacag
     4561 ctggcgtggc gctgtcaatt ctcccggcgc gttcggacgg atgcgaactt cgccgcccaa
     4621 cgcgacgatt ttatccgcca agaatgttgc gtgatcgagt tcgcttggca gttctttttt
     4681 tatcatctcg cgaagctcat ggccgagcat gccgcggccc gcggcagcgt gatagacgct
     4741 gcgcaggatc gcttccagct ccaggttcag gtcttcgttc aaaccgttaa tgagctcttc
     4801 gcgactcatc gtgacggagt ggcccaccac atgtataaca ggcagtttgt agcgccgctc
     4861 gatgcgccga ggctgatcaa gcgctaccca tcgggagcgc tgcagcggca atgtcgcgat
     4921 cacgatggag tcgtaggtct ccttgtgacg ttgcagctcg tcctcgatcg ccttcacagg
     4981 cgaaggatct cccacttccg agcggatgac tttcgcgccg gcgcgttcta attgctcctt
     5041 ggccgcctgc gccgtctgtt tggcgaccgc tgcgctatcg ccctcctgcc aatcgaggag
     5101 ttcttcggtc ggtgtagccg gcacgagcaa gacaaactcc gcatctttgt ctttctgtac
     5161 gagcttctgc accgcgctgg ttaattccgg actggatgcg gtctgattcg ctagaacgag
     5221 atatcgtctc atgtcgcacc tccagcagag taagatggag ccgacaatag gattaacgat
     5281 agataaaaac aatcaaccaa gatggcgacg ctgcccgcaa aatctaccgg atcgaaatct
     5341 catcctaagc tcagccgcgc taatttgagc tattgccaaa aaagtctcgg cgtttaatcc
     5401 ggccgccggc gcaaaactgt ccacagcgca aaaagccccg cgaacatcaa cgccgcagcg
     5461 gaaggctccg gcacttttag gaccaaatca aaaccgtatt ggaaagtgcc gggagagctg
     5521 tcctggagcc aaaaggtgta actgccggga ccgagcgggg gagcgaagcc gctcgatccg
     5581 tttgccggaa tacccatcgt cggaagaatg tttatgtctg tcgccgtagg accatagtga
     5641 atccagccga gaagtccggc tgcggttgtc gtgttggtaa gtaacgtcac ttgcgggccg
     5701 ctctgcagac cgaggaagcc gacgttgccg gcttgtgtcc ctgccagttc aatcaatgac
     5761 accaatagta gatccgatgg tacgacgatg ctgaagtaat cccgggtatt cggtgcggtg
     5821 ctgcccccgg tggttccacg tataatgttg gaacctaccg ccaccgttat ttcggtcggg
     5881 ctcaacccgc tgtccgataa ttcgccgttg acggcctcgg aataaagggt atctgcatgg
     5941 gcattggaaa acattgtcac aaccaaggcg atcgctaaca gacttttcga gcacaaacgc
     6001 aacattgtag ttcctcagtt cttaatttga gtttcgagtt tcgtaaagct ccaccaactt
     6061 ctgaattatt cttcggcgaa atgcaataat tttgcgctgt gtcccgatgc gatcgtcgcg
     6121 aaagtttgcg cgatcgtgtc agcgaatgcc taatgggtaa atttgagttt tcagctagtc
     6181 acaaagtgcc atccgcaaag cctgcgcgga ttgacagctc caggatcgat ccactagtgt
     6241 cggaattatc cgaacgaagg gggcgttacc atgaacagcc aaactagcaa gctcgttatc
     6301 gattccgacg ctcatgtcgt cgagtgcgcg catacgtggg acttcatgga caagtccgaa
     6361 gagaagtatc gtccgatacc cttggaagcg cgcgaagaag ccggtgtgcg gcaacagttt
     6421 tggctggtcg atggcaaggt ccgcggtttt cgcttcgcgg tcctctcgga cgagctcatg
     6481 gctcagcgcc aacaacaggc cggcaggaag tttgccgagc aggcggagtc gcgtgagctg
     6541 gccaacgtcg atctgcgtct cgagcacatg gatcaaacgc aagtcgatat tcaggtgttg
     6601 cacaacacca tgttcatcga gtcgtgcacc gaccgtccag cggtcgacg
//
LOCUS       DATLCH010000268         6623 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4410722, whole genome shotgun sequence.
ACCESSION   DATLCH010000268 DATLCH010000000
VERSION     DATLCH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4410722"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VMZ02_07915"
     CDS             complement(<1..429)
                     /locus_tag="VMZ02_07915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUR71897.1"
                     /translation="MANKNPVVLMSTSMGDIRIELDAVKAPITTKNFVDYVSAGHYDG
                     LIFHRVIPGFMVQGGGMDPAMKEKRNGAPIKNEAANGLKNDVGTVAMARTNVVDSATS
                     QFFINVKNNDFLNHRSTAAAEFGYAVFGRVIEGMDVVRNIE"
     gene            588..1505
                     /locus_tag="VMZ02_07920"
     CDS             588..1505
                     /locus_tag="VMZ02_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HUR71898.1"
                     /translation="MASNRQIEGILMPMPVAFKHDGAIDHKATDAIIDFYLDAGVHGF
                     FPLGTHGQGMVMEIDERKKVAEQIVNRVRGRVPVVMHIGTANTLSSVELAKHAVGLGV
                     DAVGVVPPYYYPHDDWEVYAHYKAIAQSVPGTPMFIYDNTETTWVEITPAKANKIIEA
                     IAPNPLAGIKVSFIAYDKLLGYVYQLPKTTGVFPGGIFSLYSGYSVGVRGAIHPPSTP
                     FPESCVRMYEALKADDLKAARTEHDLLTRLMQVVGKYLRSHGRGVFCELMKLRGLPVE
                     RFPRWETPPFSEKDKAELKEGLEKAGFKA"
     gene            1525..2325
                     /locus_tag="VMZ02_07925"
     CDS             1525..2325
                     /locus_tag="VMZ02_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71899.1"
                     /translation="MSSELKPLELEIGKKLSPSEAKAFTDAIKQRMYALWDKKIFGGA
                     FMRDLESGKLPFETLKLFWRNWYSYPVEVNNFHLIIYQRHQGIFARHRDLLAPYVGKI
                     SDELVNPKIPGHIEVLIKQGEQFGVSLDEMINCPVYAECRALTEFARGLVYEGSMIEW
                     WARSLNEEMFGHWAKRWREALLGKYRVKEADLHYFAVHEEADLHEHLDGLMAHGEVAR
                     QIFERLLQDGNTWYRPGWGPEYCCLTNAECVAMFHDGIYKHAQELGHF"
     gene            2433..3881
                     /locus_tag="VMZ02_07930"
     CDS             2433..3881
                     /locus_tag="VMZ02_07930"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR71900.1"
                     /translation="MPINDLREFIDVARKLGEVKDIHGAHWELEIGALTELFAFKEPS
                     PLVLFDQVPGYAPNFRIASNLINTPSRSSLTVGMPPGARPIELIAKWKELLKGVKPVP
                     PRVVNSGPILENVKSGADVDMTIFPTPHWHELDGGRYIGTADCVITGEPEEGGWVNVG
                     IYRVQIHDKNTLGLYVSPGHHARIMREKYWEKGKSCPVVVTFGQDPLLFLVAGQSMPY
                     GLSELDYYGGLRGAPIDVIRGELTGLPIPAHAEIAIEGEVPPPQEQMQMEGPFGEWPG
                     YYAHGAAKEPVIRVKRVYYRNDPILCGAPPLKPPFVTFGVPIGAAAIWNYLEKADVPD
                     IKGVWCAIGSSAAAGGAPFIIVSVKQRYHGHAQQAGLAALASRGGNYHGRFVIVVDDD
                     IDPSDMKDVIWAVSTRCDPKTAISIIDGCWSTSLDPAMHPDQRDAGNFVNSRAVINAC
                     RPYAWRDRFPPVNALSPELKRTISEKWRKELA"
     gene            3945..4571
                     /locus_tag="VMZ02_07935"
     CDS             3945..4571
                     /locus_tag="VMZ02_07935"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HUR71901.1"
                     /translation="MELLVDLFHRIYDVESLVRVGGLIGLTAIVFCETGLLIGFFLPG
                     DSLLVTAGLFAARGDLHVGSLLISLSLAAILGDTLGYNIGARAGPKLFNRPDSWLFNR
                     KHLLTTKEFYERHGPFTIVIARFVPIIRTFAPVVAGVGAMAYKRFVVYNVVGGIGWVL
                     SMILGGYFLGQLVPNIHKNIDKVILVVIFLSLLPIFIKYFKDRNKRVS"
     gene            4687..5820
                     /locus_tag="VMZ02_07940"
     CDS             4687..5820
                     /locus_tag="VMZ02_07940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71902.1"
                     /translation="MINGYKIFDADAHVMMSPQMWQDLPKEFTARRPRPLEIADSAGA
                     GIRRTGWLIEGRMEPHAYGPGAQGANTPTTVMPEFGAAPATIGSLDLSQPDKRLRDLD
                     GLGIDAQLLFPSSLYACMGSDPFFEAAMFRAYNRYAGRQCKADAKRLKWAGLLPLRDN
                     REAIEALREMQELGASAAVVFGTVGERLLSHKSFTPIWDEFARTGLPLCVHMGRSYPP
                     FDQLVESRLEAHVIAMGMPAQLGFVAVVAQGMLDRYENLKVAFLEFGAEWLFYVVGRL
                     AHYLPSYRRDPTVQSMGRLPEKAIEEYLKSGRFFIAPEADDPLLVQEIALVGAEHILY
                     SSDYPHGEGRDNAAHEILQRQDLTDAQKRSILYDNTVRFCGEP"
     gene            complement(6003..>6623)
                     /locus_tag="VMZ02_07945"
     CDS             complement(6003..>6623)
                     /locus_tag="VMZ02_07945"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71903.1"
                     /translation="DLRGKPIGITGAGTYSEFAVKAFLKKNNINPDKDVVLRAIGGTV
                     LRAAAIEKGIIAAAPFSTEDAVRLIRSGYAVISNMNESLGIPQNIIVTRNEVLDKYPD
                     TSKRVLKAYIQGMQLAKFNKREAVKAGYDAGMQGEPDIVSAAWDLYSPGLTSGLSIAV
                     PGIQQMLDEDIRTGLVEKNFTVDRVINDRILKIAQQELRAEGKLRQ"
BASE COUNT         1439 a         1842 c         1837 g         1505 t
ORIGIN      
        1 ctcgatatta cgaacgacgt ccatgccctc gatcacccgg ccgaaaaccg catagccgaa
       61 ctccgccgcc gcggtgctgc ggtgattcaa gaagtcgtta ttctttacat tgatgaaaaa
      121 ttgcgaggtc gcgctgtcga ccacattggt acgcgccatg gcgacggtac cgacgtcgtt
      181 tttcaagccg ttggccgctt cgttcttgat cggtgcgccg tttctctttt ccttcatcgc
      241 gggatccatg ccgccgcctt ggaccatgaa cccgggaatg acgcggtgaa agatcaaacc
      301 gtcgtagtga ccagcactga cgtaatcgac aaagtttttg gtggtgatcg gcgcctttac
      361 cgcgtctaat tcgatgcgga tatcgcccat gcttgttgac atcaatacta ccgggttctt
      421 attagccatc gttgcctccg aaaaagtatt caccaagcct aacatgtcgc gccgaggact
      481 tatagcccta gcgcttgcag gcacgcgagt tggtggaaat ctggtcagcg gtgcgctatg
      541 ggtgagcaat caattaaaag gattgaacga tatcgaaagg agcatttatg gctagcaata
      601 gacaaatcga gggcattttg atgccgatgc ctgtggcgtt caaacacgac ggcgccatcg
      661 atcacaaagc gaccgacgcg attatcgatt tttatttgga tgccggtgtg catggatttt
      721 ttccgttggg aacccatggc caaggcatgg ttatggaaat cgatgagcgc aagaaggttg
      781 ccgagcagat cgtcaaccgt gtcagggggc gtgtgcccgt ggtcatgcat atcggcacgg
      841 caaacaccct ttcatcggtc gagctggcca agcacgccgt gggtttaggc gtcgatgccg
      901 ttggcgtcgt gccgccgtat tattatcccc acgacgattg ggaagtgtat gcccactaca
      961 aagccatcgc ccaatccgtg cccggcacgc cgatgttcat ctatgacaac acggaaacca
     1021 cgtgggtcga aatcacgcca gccaaggcca acaagattat cgaagcgatc gcacccaatc
     1081 ctttggccgg tatcaaagtg tcctttatcg cctatgacaa actgctcgga tacgtctatc
     1141 agctgccgaa aaccaccggt gtcttccccg gcggcatttt ttcgctatac agcggatatt
     1201 ccgtcggtgt gcgcggcgcg atccacccgc catctacgcc cttcccggaa tcctgcgtca
     1261 ggatgtatga agcgctcaaa gccgacgatc tcaaggcggc gcgcacggaa catgatctgc
     1321 tcacccgact catgcaggtg gtcggcaaat atctgcgcag ccacggccgc ggtgtttttt
     1381 gtgaactgat gaagctgcgc ggacttccgg tggaacgctt cccgcgctgg gaaactccgc
     1441 cgttcagcga gaaagacaaa gctgaattga aggaaggcct ggaaaaagcc gggtttaagg
     1501 cctaacccga caaggttaat gtttatgtct agcgagttaa aacccctgga attagaaatc
     1561 ggcaaaaagc tttcgcccag tgaggcgaaa gctttcaccg acgctatcaa acaacgcatg
     1621 tacgcgctgt gggataaaaa gattttcggc ggtgctttca tgcgcgatct cgaatccggc
     1681 aagctgccct tcgagacact caagttattc tggcgcaact ggtacagcta cccggtggag
     1741 gtgaacaatt ttcacctgat tatttatcag cgccaccagg gcatcttcgc gcgccatcgc
     1801 gacctcttgg cgccttatgt cggaaaaatt agcgatgagt tggttaatcc gaaaattccc
     1861 ggccacatcg aagtgctgat caaacagggc gagcagttcg gcgtgtccct cgacgagatg
     1921 atcaactgcc cggtctacgc cgagtgccgt gctttgaccg aattcgcgcg tggtctggtt
     1981 tacgaaggca gcatgatcga atggtgggcg cgctcgctga atgaagagat gtttggccat
     2041 tgggccaagc gctggcgtga agcgctcctc ggcaaatacc gcgtcaagga agccgacctg
     2101 cattatttcg cagtccacga ggaagccgac ctgcacgagc atctggatgg ccttatggct
     2161 catggcgaag tggcacggca gatctttgaa cgactgttgc aggacggcaa cacttggtat
     2221 cgcccgggct ggggaccgga gtactgctgc ctcaccaatg ccgaatgcgt cgccatgttt
     2281 cacgacggca tctacaagca cgcgcaggaa ctgggacact tttaaataat ggaaaatgga
     2341 gaattgataa tggagaacgg aagagtaagg gaccgtgcga ttgtccgaaa atcaattatc
     2401 cattatccac tttcaatttt ccaatcttag ccatgccgat caacgattta cgtgagttta
     2461 tcgacgtcgc ccgcaagctc ggcgaagtga aagatattca tggcgcccat tgggagctcg
     2521 agattggcgc gttgacagaa ttgttcgcat tcaaagaacc ttcaccgctc gtgctcttcg
     2581 atcaagtccc cggctatgcg ccaaactttc gtattgcgtc gaatctgatc aacacgccgt
     2641 cgcgttcgag cttgactgtt ggcatgccgc ccggtgcccg ccccatcgaa ctcatcgcta
     2701 aatggaaaga gttgctcaaa ggcgtaaagc ccgtgccgcc gcgggtagtc aacagcggcc
     2761 caatacttga gaacgtgaaa tccggcgccg acgtcgacat gacgattttt ccgacgccgc
     2821 actggcatga gcttgacggc ggccgctaca tcggcaccgc cgactgtgtc atcaccggcg
     2881 aacccgaaga aggcggttgg gtcaacgttg gtatttatcg ggtgcagatc cacgacaaga
     2941 acactttagg tctttatgtt tccccgggac accatgcgcg gatcatgcgc gagaaatact
     3001 gggagaaagg caagagctgt ccggtggtcg tcaccttcgg ccaggatccg ctgctatttt
     3061 tagttgccgg tcagtcgatg ccgtacggct tgtccgagtt ggattattat ggcggcttgc
     3121 gcggcgcgcc gatcgatgtg attcgcggcg aactcaccgg tctgccaata cccgcccatg
     3181 ccgagatcgc catcgaaggc gaagtgccgc cgccccagga acaaatgcaa atggaaggac
     3241 cgtttggtga atggcccggg tactacgccc acggcgctgc caaggaaccg gtcatccggg
     3301 tcaaacgagt ttattaccgc aacgatccga ttctctgcgg cgcgccgccg ttgaaaccgc
     3361 ccttcgttac attcggtgtg ccgatcggcg ccgcggcgat ctggaattat ttggaaaaag
     3421 ccgatgtgcc cgacatcaaa ggcgtctggt gcgccatcgg cagctcagca gccgccggcg
     3481 gagcgccgtt tatcatcgtt tcagtaaagc agcgttatca cggtcacgcg caacaggccg
     3541 gcctggctgc gctagcctct cgcggcggca actatcacgg ccgcttcgtc atcgtcgtcg
     3601 atgatgacat cgatccatcg gatatgaagg acgttatctg ggcggtgtcg acgcgttgcg
     3661 atcccaaaac cgccatcagt atcatcgacg gttgctggag cacgtcgctc gatccggcga
     3721 tgcacccgga ccagcgcgat gccggcaact tcgtcaatag ccgtgctgtc atcaatgcct
     3781 gccggccgta tgcatggcgc gaccgctttc cgccggtcaa tgccttgagt ccggaactca
     3841 agcgtacgat cagcgaaaag tggcgcaaag aattagcctg agctggctaa agtcgcggca
     3901 ccggccaatt gcctctctgc cattagcgtg gcaccgtaag cgttatggaa ttactcgtgg
     3961 atctgtttca tcgcatctac gatgtcgagt cgctggttcg cgtcggcggg ctcatcggtc
     4021 tcacggcaat cgttttttgt gaaaccggtt tgttgattgg cttttttctg ccgggtgatt
     4081 cgttattagt gacagcaggc ctgtttgccg cgcgcggcga ccttcatgtc ggctcgcttt
     4141 taattagctt aagtctcgcc gctattcttg gtgacacatt gggttacaat attggcgcgc
     4201 gtgccggccc taaactcttc aatcgtccgg actcctggct cttcaatcgc aaacatctgc
     4261 tgacgaccaa ggaattctac gagcgccacg gaccgttcac catcgtcata gcccgttttg
     4321 taccgattat cagaactttt gcgccggtcg ttgccggcgt cggcgccatg gcttacaaac
     4381 gcttcgttgt ctacaacgta gttggcggca tcggttgggt gttgtcgatg atcctgggcg
     4441 gctattttct cggccaactc gttcctaaca ttcacaagaa catcgacaag gttattctcg
     4501 tcgtgatctt cctttcgctg ctgccgattt tcatcaagta tttcaaagac cgaaataaac
     4561 gggttagctg aacgctgcgc cggtcactgc atttgcagcc gcacacgtac atcgattaag
     4621 aagaatattt ggcggacgac ccatcgagtg tggactcggc ggcactttca gcgccaagga
     4681 gttgaaatga tcaacggcta caaaatcttt gacgccgatg cccatgtcat gatgagcccg
     4741 cagatgtggc aagatttgcc caaggaattt accgcgcgcc gaccgcggcc gctggagatc
     4801 gccgatagtg caggagctgg aatccgccgc accggctggc tcatcgaagg acgtatggaa
     4861 ccgcacgcct atggtccggg cgctcaaggg gcaaacacgc cgaccacggt aatgccggag
     4921 tttggcgccg cgccagccac cattggatca ctcgatttgt cgcaaccaga caagcggttg
     4981 cgcgatctcg acggcctcgg catcgacgcc caactgctct tcccttcgag tctttatgcc
     5041 tgcatgggca gtgatccatt tttcgaagcg gcgatgttcc gcgcctataa ccgctatgcc
     5101 ggtcgtcaat gtaaagccga tgcgaaacgc ttgaagtggg ccggcttatt acccttgcgc
     5161 gataaccgtg aagccattga agcgctccgt gaaatgcagg aactgggtgc cagcgctgcc
     5221 gtcgtcttcg gaacggtcgg cgagcgattg ctatcgcaca aaagtttcac gccgatttgg
     5281 gatgaatttg cccgcaccgg cctgccgttg tgcgtgcaca tgggacgcag ttacccgccc
     5341 ttcgaccaat tggtggaaag tagactcgag gctcacgtca tcgccatggg catgccggca
     5401 caactgggtt tcgtcgccgt cgtcgctcag gggatgctcg accgctacga aaatctaaaa
     5461 gttgcttttc tcgagtttgg cgccgaatgg ttgttctacg tcgtcggccg attggcacac
     5521 tatctgccga gctatcgccg agaccctacg gtacagtcca tgggccgact accggaaaaa
     5581 gccatcgaag agtatcttaa gtccggccgc ttcttcattg cccccgaagc agacgatccg
     5641 ttgctggtac aagaaatcgc gctggtcggc gcggagcata ttttatactc atcggattac
     5701 ccgcatggcg aaggtcgcga taacgcggcg cacgagattt tgcaacggca ggatctgacc
     5761 gacgcgcaga agcggagtat cctgtacgac aacacagttc ggttttgtgg cgaaccctaa
     5821 ctcaccatcg aaggatgatt gaaatccgaa cagcaggtgg attgagaaaa catctgtcat
     5881 gccggcgcac gccggcatcc agggtctatt tcgcttcgcc cctaagaagc ctggattccg
     5941 gcttgcgccg gaatgacgga aggacaaaac actcccagtc ggtgcattca gaacacctcg
     6001 acttactgtc ttaacttacc ttcggcgcgc agctcttgtt gggctatttt tagaatgcgg
     6061 tcgttgatga cgcgatctac ggtaaagttt ttctcaacca gtccggtgcg gatatcttcg
     6121 tcgagcatct gctggatgcc cggcaccgcg atcgacagac cgctggtgag cccgggcgag
     6181 taaagatccc aagcggcgct gacgatgtcc ggttcgccct gcatgccggc atcgtagccg
     6241 gctttgacgg cttcgcgttt gttgaacttc gcgagttgca tgccttgaat ataggctttg
     6301 agcacacgct tgctggtgtc gggatactta tccaggactt cgttgcgcgt aacgattata
     6361 ttttgcggga ttcccagcga ctcgttcata ttgctgatga cagcataacc ggaacggatg
     6421 aggcggaccg cgtcctcggt ggaaaatggc gctgccgcga taatcccctt ctcgattgcc
     6481 gcggcgcgca ataccgtacc gccgatggcg cgcagcacga cgtccttatc ggggttgatg
     6541 ttgttcttct tgagaaaggc tttgacggcg aattccgaat aggtgccggc gccggtgata
     6601 ccgatcggct tgccgcgcaa atc
//
LOCUS       DATLCH010000269         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_717040, whole genome shotgun sequence.
ACCESSION   DATLCH010000269 DATLCH010000000
VERSION     DATLCH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717040"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..2119)
                     /locus_tag="VMZ02_07950"
     CDS             complement(257..2119)
                     /locus_tag="VMZ02_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HUR71904.1"
                     /translation="MADNELTPLQSILAALAPQGRSGLLPALHAAQRIDGWISEPVAT
                     AVSRALGVPLADVCGVIDFYELFNRQPVGRTIVRVCSAPVCALAGAEAVIEALCRHLQ
                     TEPGDMSSDGAFTVEHAPCLGLCDHAPAVLIGEKAIGAANPAQAEAICTQTAGKALSI
                     VDGDIRMLTSNCGAGRPTSWADYTTRGGYAGLKKALSTTPQATLAEVKSAGLVGRGGA
                     AFPTGVKWEDAANAPDGPKYIVCNADESEPGTFKDRILMEEDPHRIIEGMIIAAYAVG
                     ASQGYLYVRGEYPRAFRIMSDALAEARQAGAIGVSIFHTGFNFDIEMRLGAGAYICGE
                     ETALLESIEGKRGLPRIKPPFPTTHGLFGKPTVINNVETFCNVPLIVASGAAEYRRIG
                     TERSPGPKLFCVSGQVQRPGLYEVPFGVTLRHLLFDLAGGLDPGRKLQAVLMGGAAGA
                     FAIEKDLDVILSFENLSAAGLPLGSGAVMVFDDSADLRDALKRLAHFFADESCGKCYP
                     CQLGTRRQYEILERNAAALPLTGDRERLLDIGHTMIDASLCGLGQTAPTAILSAMNRR
                     DFRASVFESPAPAKDPPPSRAFPKSYGGCVRPGGSMSKLSCPRSPGSSMPARAV"
     gene            complement(2413..3198)
                     /locus_tag="VMZ02_07955"
     CDS             complement(2413..3198)
                     /locus_tag="VMZ02_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71905.1"
                     /translation="MKEPKAGEKFTEQEAKEYVDHLYKGLYKYAHDKIYKGAFMTQLR
                     DGKLPLPVMRQFFKNWGHFSLEVNALNAVSYYTHLPFFVRHFDLLGPFCAKIADELIS
                     PKAPGHVLVLLQTAEKMGLNKKEMLEDPYLPAARSINDFCHKIFIDGSIVELWGLHVF
                     EESLSMWSGQWFTALTTHYGFSKKDAIYFSTHEEADMDTHKLGDSDEEAMGHGAFNRT
                     VLTRALQESVEFRAGYSLEYCAMTMVDLHAQMKQAALDNPYNP"
BASE COUNT          733 a          932 c          948 g          747 t
ORIGIN      
        1 gaaagccggg ataggactta gcatgatcgc ttattccagt aatgtttccc aattcgattt
       61 ctcacccagt gctccaaagg acttctcatg actgatgtca cgctgacgat tgacggcgct
      121 tccgttacgg ttccagcgga aaccaccatt ctgaaagcgg ccgagcagct tggccgcgaa
      181 cttccgacga tttgctacca tgatcactgc acggcaaatg gcttgtgccg catttgcgtg
      241 gtcgaagtcg aaggggtcaa accgctcttg ccggcatgct tgagccgggc gagcgagggc
      301 atgagagttt cgacatcgag cccccgggcc gaacgcagcc gccgtacgat tttggaaatg
      361 ctcgactcgg cggtggatct ttcgcaggcg ccggagattc aaaaactgat gcgcgaaagt
      421 cacgccgatt catcgcgctt aatatcgccg tgggcgcagt ctggccaagg ccgcataggg
      481 atgcgtcgat catcgtgtgg ccaatatcca acaagcgctc gcgatcgccg gtaagcggta
      541 gcgcggctgc gttccgttcg agaatttcat attgccgccg cgtgcccaat tggcatggat
      601 aacatttgcc gcagctttca tcggcaaaaa agtgtgctaa tcgcttcaag gcatcgcgca
      661 ggtcagcgct gtcgtcgaag accatgactg cgccagaacc gagcggcaag ccggccgcgc
      721 tcagattctc gaacgagagg atgacatcta aatctttttc tatggcaaag gcgccggcag
      781 caccgcccat aagcacagct tgaagtttac gtcctggatc gagcccaccg gcgaggtcga
      841 acagcagatg acgcagggtg acgccgaacg gcacttcgta aaggcctggc cgctgaacct
      901 gacccgagac gcagaaaagt ttcgggccgg gcgagcgctc agtgccgatc ctgcgatatt
      961 cggccgcgcc gcttgcgacg atcagcggga cgttgcagaa agtttccacg ttgttgatga
     1021 cggtgggctt accgaataaa ccgtgagtcg tgggaaatgg tggcttaatg cgaggcaatc
     1081 cacgtttccc ttcaatcgac tccaacagcg ctgtctcctc gccgcaaata taggcgcccg
     1141 cgcccaaacg catctcgata tcaaagttaa agcctgtatg aaaaatactt acgccaatag
     1201 cgccggcttg gcgcgcctcg gcgagcgcat cgctcatgat ccggaaagcg cgcggatact
     1261 cgccgcggac ataaaggtaa ccctggctcg cgcctacggc ataggccgcg atgatcatcc
     1321 cttcgatgat ccggtgcgga tcttcctcca tgagaatgcg atctttgaag gtgccgggct
     1381 cggattcgtc tgcgttgcag acgatatact tgggtccatc tggggcgttt gcggcgtcct
     1441 cccatttgac gccggtggga aacgcggcgc cgccgcggcc aaccaaccct gccgatttca
     1501 cttcggcgag cgtggcttgc ggcgtggttg acagcgcctt tttaaggcca gcataaccgc
     1561 cgcgcgttgt atagtcggcc catgacgtgg gacgaccggc gccgcaattg ctcgtgagca
     1621 tgcggatatc accgtcgaca atgcttaatg cttttcccgc ggtttgtgtg caaatcgcct
     1681 ctgcctgtgc tgggttcgcg gctccgatgg ctttttcgcc gatcaggacg gcgggcgcgt
     1741 gatcgcacaa gcccaggcag ggcgcatgct caaccgtgaa cgcaccatcg gaactcatgt
     1801 cacccggttc agtttgcagg tgacggcaca gcgcttcaat caccgcttcg gcaccggcaa
     1861 gcgcgcatac cggcgcgctg catactcgca cgattgtccg ccccaccggt tgtcgattga
     1921 acagttcata aaaatcgatg acgccacata catcggcgag cggtacgccc aatgcccggc
     1981 tcactgcggt cgccaccggc tcggaaatcc acccatcgat tcgttgcgcc gcatgcaagg
     2041 ccggcaggag cccagaccgg ccctgcggcg ccaaggcggc gagaatcgat tgtagagggg
     2101 taagttcgtt atcggccatc taacaattag caatcgtcaa tggatcattc gctcgaatcg
     2161 ttcttagtat aattcgaaaa tagtcacacg gcactgaaca gttgctcgct tctcgatcgt
     2221 gcccgagttg gtagttgtct gcaagccgtg ttttggttaa cagaaatgca cgctgctggc
     2281 gagattggcg gaatgtcgag ccaggtgtgg cttactcgct tggctgggat tgcgcccatt
     2341 tgatcttatg gcgctcacaa aggttgcgag tactgtcctg agctaatcag aagccccggt
     2401 gaatttgcca atctatggat tgtacggatt atcgagagcg gcttgtttca tttgcgcatg
     2461 cagatcgacc atcgtcatgg cgcagtattc cagagagtag ccggcgcgaa actcgacact
     2521 ctcctgcaaa gctcgcgtaa gcacggtgcg gttgaaggcg ccgtgaccca tggcttcttc
     2581 atcgctgtcg ccgagcttgt gggtgtccat gtcggcttct tcgtgggtcg agaaatagat
     2641 cgcgtctttt ttgctaaacc cataatgggt tgtcagagcg gtgaaccact gacccgacca
     2701 catcgacagc gactcttcga aaacatgcag accccagagt tcaacgatgg aaccgtcgat
     2761 aaaaatcttg tggcagaagt cattgatcga gcgggcggcg ggcagataag ggtcttcaag
     2821 catctccttc ttgttcaagc ccattttctc tgcggtttgc agcagtacca gcacatgccc
     2881 cggcgccttg ggcgagatta gttcgtcggc aatcttggcg cagaacggcc cgagtaggtc
     2941 gaagtggcgc acgaaaaacg gcaaatgggt gtaataggac acagcattga gcgcgttaac
     3001 ttccagagag aaatggcccc agttcttaaa aaactggcgc attaccggca acggcagttt
     3061 gccgtcacgc agttgggtca taaaggcgcc cttgtaaatc ttatcatggg cgtacttgta
     3121 aagccctttg tatagatgat cgacgtattc tttggcttct tgctcggtaa atttttcacc
     3181 agctttgggt tctttcatgg cgtaactccg atcgcgcaaa gggattcgat aaacgcctat
     3241 cgtgttacca tccgggtttt ggcgagtcaa ggtaagtggt cgaattgcaa tggcattagg
     3301 gtgagatatt atgaccccat ggctacccaa cccagccgcg aattagaaac ctttccaaat
//
LOCUS       DATLCH010000270         4102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10796570, whole genome shotgun sequence.
ACCESSION   DATLCH010000270 DATLCH010000000
VERSION     DATLCH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10796570"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..984)
                     /locus_tag="VMZ02_07960"
     CDS             complement(145..984)
                     /locus_tag="VMZ02_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71906.1"
                     /translation="MPTAEIGNVTIYYESQGEGDAVLLCPPSWWPCDTWNVGVVPFLS
                     KNFRTIIFDCRGTGNSSKPDHGYTIEQFANDCAGLLEHLKISRCHAVGFALGGQIAQA
                     LAIARPDLVQTLTIAAAGAGVKATDGGPRVVRNTDDDEIREHGFEQFIRGHIDNTHMA
                     FNDKFFAAHADVVKALSDALWKRQTSPEQHRYHFAARRTWDTLGKAAEVKVPTLILCG
                     AEDDVNRGGSTPVGTARKLSELLPGSELKLVADVKHMTFWDGDGALHALQNFLARHPI
                     SGS"
     gene            1545..2210
                     /locus_tag="VMZ02_07965"
     CDS             1545..2210
                     /locus_tag="VMZ02_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR71907.1"
                     /translation="MSNPFVESVLKEIIHPGVEQLMNTRFFTELRAGKLSQKRLQGFA
                     VQHYLSNHVINKGLAFSMVRNANNQAMYEQFIELFTEEQSHPALMRRFGIAMGLADAD
                     FDNGIMIYECVAHTGAIVRGMLVGAPAETRVGALVNETMVQRYSEEFDAALGKHYGLD
                     SRARTFFAVHGKVDMEHTAMAADTVAKFAITDRDKEVVRAAAKNMVRFKVAKFDGIYD
                     TYA"
     gene            2279..3259
                     /locus_tag="VMZ02_07970"
     CDS             2279..3259
                     /locus_tag="VMZ02_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71908.1"
                     /translation="MKRSWVLNSLMAAVISIPGTIWPAEKSAKLTTFRIAYVSRSTLD
                     MPFVIARDRGFFREEGLEPEFIFMKAIQTVQAMLAGGVDFGTATGTAISAAVNGADVR
                     VVFALSDKPSFDMISLPSITSVAQMRGKKLGISAPGSLTEIIARQIFIANKIPLDQVT
                     MLPLGTSDITWVALKAGTIDATMLQIPQNFIAVDEGFRKIAAGADVYRAVQGGLTTTK
                     AVINEKPELVTKVIRATQKALRLFRNDKKYGIEFIRGPFLDMGKDRDKYAERVYEAAV
                     GYLSASGTVDEKLQREMITTAAQRTKLAQPPPPERVFDFSFAQKVGETIK"
     gene            3385..>4102
                     /locus_tag="VMZ02_07975"
     CDS             3385..>4102
                     /locus_tag="VMZ02_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71909.1"
                     /translation="MKYSTERILTTHAGALPLPADLKELHNARTAGTAVDEQVFSKRV
                     TGAVADIVRKQIACGLDIINDGEQGKSNFSRYARDRLSGFIEREVKASDHTSTIYARD
                     LTEFSDYFNARTFHRGDNVKRVFCNAPLKYVGHASLKAEIADFQAGLQGQQYTEAFLP
                     AVAPGTIEHWMKNDYYPNDEAYLVAIADAMHEEYKAIADAGFLLQIDDPDLPDGWQFM
                     SKMTVPEYRKYAQLRVEALNH"
BASE COUNT          943 a         1210 c         1062 g          887 t
ORIGIN      
        1 gctgcgctcc ggcagcgctg gccaacaaca acaccatcgc aatagcgata tatccattcc
       61 aatcatgtcg ggacggcttc attgtcaatc tcctgaaacg cgctgggtca gagttcgagt
      121 gtttgacgtt caattgtttg ttgcctaact gccactgatc ggatggcgtg ccaagaaatt
      181 ctgcagtgca tgcaacgccc catcgccatc ccaaaacgtc atatgcttga cgtcggccac
      241 cagtttgagt tcactgccgg ggagcagttc gctaagtttg cgcgctgtgc cgaccggtgt
      301 gctgccgccg cgattcacat cgtcctctgc gccgcataaa atcagcgtcg ggacttttac
      361 ttccgccgcc tttcccaaag tatcccaagt acgccgcgca gcgaaatgat agcgatgctg
      421 ctccggactg gtttgccgtt tccacaacgc atcagacagt gctttgacca catcggcatg
      481 cgcggcgaag aacttgtcgt taaacgccat gtgcgtattg tcgatatgac cgcggatgaa
      541 ctgctcgaag ccatgctcgc gaatctcatc atcatcggtg ttgcgcacca cccttggccc
      601 accatcggtc gccttcactc cggcgccggc cgcggcaatc gtcaacgtct ggaccaaatc
      661 tggccgggcg atcgccagag cctgggcgat ctggccgccc agcgcgaaac caaccgcgtg
      721 acaccgagaa atttttaagt gctctaatag ccctgcacag tcattggcga actgctcaat
      781 ggtgtagccg tgatccggtt tactgctgtt gccggtgccg cgacagtcaa aaatgattgt
      841 gcgaaaattc ttcgacagaa acggcactac gccgacattc caggtatcgc aaggccacca
      901 agacggcggg cagagtagga ctgcgtcacc ctcgccttgg ctttcatagt aaatcgtcac
      961 attgcctatt tcagccgtcg gcatttttca cctcaaatca aattaactca gaaaattcta
     1021 gcatcatctg ttggagagag atttctccct gcggtcgaaa tgacataaat tagtattctt
     1081 aaccttgcgc cttggcctgt gcgggagatc ttccgcatcc accgtagccg caagcgggag
     1141 ctcgcttctt tcgcagccca tttgtagtcc gacattgacc gcgatctcaa acgaaatgtt
     1201 ctcctcgaag cttgatcgga tgacgcaagc agaaactctt cgagcgccgt cagcccgccg
     1261 ttgccatccc agaaggtcat atgcgtgggt gttgggaacc aaacaaagct cagctcgagg
     1321 tgtcaattca gccaagcgcc ggcgcaagca gtaccggctc gccgcggccc tgcacttcgt
     1381 agtatatttt taggctagtt acttctgcga ttgccattta acagcccgac aacgtcctca
     1441 cgtttagtcg ccgcaccgct cgctgtcaat ccaattgtca gcagcttgac agtcgatcct
     1501 ttcctctgcc actgttcttc aaataatcga ttagaggagc ggtaatgtcc aacccattcg
     1561 ttgaatccgt actcaaggaa atcattcatc ccggcgtaga acagctgatg aacacgcgct
     1621 tctttaccga actgcgcgcc ggcaagctgt cccagaagcg gctccagggt tttgccgtgc
     1681 agcactattt atcaaaccac gtgatcaaca aagggctggc tttttccatg gtccgcaacg
     1741 ccaacaatca ggcgatgtac gagcagttca tcgagctgtt caccgaggaa caatcccatc
     1801 cggcgctcat gcgccgcttc ggtatcgcca tgggccttgc cgacgccgac tttgacaacg
     1861 gcatcatgat ctatgaatgc gtcgcccaca ccggagcgat cgtccgcggt atgctcgtcg
     1921 gcgcacccgc ggaaacccgc gttggcgcat tggttaatga aaccatggtg caacgctact
     1981 cggaagaatt cgatgcggcc ttgggcaaac actacggcct cgactctcgg gcgcgaacct
     2041 ttttcgcggt ccatggcaaa gtggatatgg aacataccgc catggcggcc gacacggtcg
     2101 cgaaattcgc catcaccgat cgagacaaag aagtggtccg cgccgcggcg aagaatatgg
     2161 tgcgcttcaa ggtcgccaaa ttcgacggca tatacgatac ctatgcgtga gacactctag
     2221 cgtccggggc gtagagttta gccgtcgcca acccgctcaa ctagggaggg attagccggt
     2281 gaaacgatct tgggttttga actcgttgat ggcggcggtg atatctattc ccggaacgat
     2341 ttggccggcg gagaaatccg ccaagctcac cacgttccgc attgcttacg tgtcacgcag
     2401 caccctcgat atgccgtttg tcatcgcccg cgaccgcggg tttttccgtg aggagggttt
     2461 ggagccggag tttattttca tgaaagccat tcaaaccgtg caggcgatgc ttgccggcgg
     2521 cgtcgacttt ggcaccgcca ccggcacagc gatcagcgcc gcggtcaacg gtgccgatgt
     2581 ccgggtcgta ttcgcactgt ccgacaaacc gtccttcgat atgatctcgc tgccgagcat
     2641 caccagcgtc gcacaaatgc gtgggaaaaa actcggcatc tcggcgcccg gatcgctgac
     2701 cgaaatcatt gcccggcaga tttttattgc caacaagatt cctttggatc aagtcaccat
     2761 gttgcccttg ggcacgagcg atatcacctg ggtcgctttg aaagccggca ccatcgacgc
     2821 caccatgctg caaattccgc agaattttat cgccgtcgac gagggttttc gcaagatcgc
     2881 cgccggcgcc gatgtctatc gcgccgttca aggcggcctg acgaccacaa aggcggtgat
     2941 caacgaaaaa cccgaactgg tgactaaagt gatccgcgcc acgcagaaag cacttcggct
     3001 gtttcgcaac gataaaaaat acggcatcga gttcattcgc ggtccttttc tcgatatggg
     3061 taaagatcgc gacaagtatg ccgagcgcgt ctatgaggcc gcggtgggtt acttatcggc
     3121 ctcgggcacc gttgatgaaa aacttcagcg tgaaatgatc accaccgccg cccagcgcac
     3181 caagctcgcc cagccgccgc ctccagagcg ggtattcgac ttcagcttcg cgcaaaaggt
     3241 cggggagaca attaaatgat tactttgaga tccgaaacga tttggaacaa ttggaacgac
     3301 tgaaacctag gaaccacgag ttttgttcca atcgttccaa ttgttccagc ggttccaatc
     3361 gtgtttagga aagtaggaat gtaaatgaaa tatagtaccg agagaatttt aaccacccac
     3421 gccggcgcct tgccgctccc cgccgattta aaggagctgc acaacgcccg caccgccggg
     3481 actgccgtag acgagcaagt tttcagcaaa cgcgtcaccg gcgccgtggc cgatattgtc
     3541 aggaaacaaa tcgcctgcgg cctggatatc attaacgacg gcgaacaggg caaatccaac
     3601 ttctcacgtt acgcccgtga tcgtttgagc ggctttatcg aacgcgaagt taaagcgagc
     3661 gatcatacct cgacgatcta cgcgcgggat ttgaccgagt tcagcgacta cttcaacgcc
     3721 cgcaccttcc accgcggcga caacgtcaaa cgtgtgtttt gcaacgcgcc gttgaaatac
     3781 gtcggtcatg catcactgaa ggcggagatc gccgacttcc aggcaggcct gcaagggcag
     3841 caatacacgg aagcgttcct tcctgcggtc gctcccggca ccatcgagca ctggatgaaa
     3901 aacgactatt acccaaacga cgaggcctat ctcgtcgcga ttgccgatgc catgcacgag
     3961 gaatacaaag ccatcgccga cgccggcttc ctgctgcaaa tcgacgaccc cgatttgccc
     4021 gacggctggc agttcatgtc gaagatgacg gtgccggagt atcgcaagta cgcccaactg
     4081 cgtgtcgaag cgctcaatca cg
//
LOCUS       DATLCH010000271         5419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2201414, whole genome shotgun sequence.
ACCESSION   DATLCH010000271 DATLCH010000000
VERSION     DATLCH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201414"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VMZ02_07980"
     CDS             complement(<1..184)
                     /locus_tag="VMZ02_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HUR71910.1"
                     /translation="MADYKEIIYQKQRGGVLITLNRPEALNAITRPMLKEFHDALDQA
                     EADPEIRAVVITGAGRA"
     gene            complement(308..1324)
                     /locus_tag="VMZ02_07985"
     CDS             complement(308..1324)
                     /locus_tag="VMZ02_07985"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71911.1"
                     /translation="MVYSLLSTFFVAVVGLWTIQPANAQERLRVGLSSVSATQGAMWV
                     AEERGLFKKYGVDPEVIVIGGGASRVISSLLAGEIQFSVGGGDSVIRAALRGADPVLA
                     VSPLKTGLQRLMVRSEIKTPADLKGKKIGVTRFGSASHWVLQLFLRKWNMRNDDIQIL
                     QLGSSPANLASLDSRAIDGAVLTLPTFFVAEERGYRLLADPADLDIVYLQNSIDTTRS
                     YLKRNRDQAVRFIKGYCEGMAYFRKNKRDSVAVMQKKLRIQSAQEKDTKYLEMSYDLL
                     ANKFYNQIPYATPKAVETTLDFVAIEEPKAKGADPRQFLDESIVRELETSGFFKNLYE
                     GEYR"
     gene            complement(1332..1478)
                     /locus_tag="VMZ02_07990"
     CDS             complement(1332..1478)
                     /locus_tag="VMZ02_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71912.1"
                     /translation="METNFVVAAQAGIQFFLPLQHKRRWMPACAGMTDNGFTGTQWVS
                     ITAK"
     gene            complement(1555..2553)
                     /locus_tag="VMZ02_07995"
     CDS             complement(1555..2553)
                     /locus_tag="VMZ02_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71913.1"
                     /translation="MKLNLAIAILVGAALVHAHVHAQEPTKIPFPYGPLGLNSVPFVI
                     AKEARLFEKNGLTVDMVYVGASAVMVQSMLSGSANLAAFGGPAVITNVLSGGDIIQIA
                     ALLPYFTQSLMVRGDIRDVRSLQGKKIGITRFGSVTDFALRTLLEKNNLKDVSALQMG
                     GFPEAVAALIRGAIDGAVLSPPHTFRLLKEGYRELASPKDLRALGSGFISQGIVARRS
                     YASTHRDLVVRLIKATVEAMKFINANEDFTKRLIGKYLGITDTEVLRQSYAYVTDNFA
                     RDPAVPENVMQSMVQRMAQLNMIDTKAAQATPTSAYYDNSFVNEIKSSGFLENVWK"
     gene            2681..3487
                     /locus_tag="VMZ02_08000"
     CDS             2681..3487
                     /locus_tag="VMZ02_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71914.1"
                     /translation="MPKVKINDIELYYEEHGSGRPMVFLSETACDGEVWKINQVGEFS
                     RDHRVITFDYRGTGQSSKPSIDYTTQMFCEDVIGLMDYLKADQAVVVGHSMGGRVAQL
                     MALDHPKKVHKLVLASTGAHYPQTKGLPLKIMKEMIEWGYEKYERDHTILVGWTDNYV
                     KQHPEKIEAYLKVRMTNLCPVEFYLRHLIARQSHDTSQRLKDIKQPTLILVGDGDVNM
                     TSEMNHRMSTDVLARGIPNSKVVLLPNERHSYFFSNPDAAHRAIREFIKD"
     gene            3834..4637
                     /locus_tag="VMZ02_08005"
     CDS             3834..4637
                     /locus_tag="VMZ02_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUR71915.1"
                     /translation="MPTVRTNGAEIYYETHGHGQPFLFFSETACDGQIWKMYQVPEFA
                     IDHQVIVHDYRGTGRSSKPSIDYTTRMFCDDAVALLDHLNAKDAIVLGHSMGGRVAQL
                     LALDHPQRVKKLILASTGASFPGGAGLPLQMCKEMIEWGYEKYVREHTMHVGFTDEFA
                     KSQPERLEAFFRQRLDNICPVEFYLRHVLSRQGHDTSARLKDIAIPTLVIVGELEGDP
                     KVVMTHRMSSDILAKGIPNAKFVLLPKQKHNYFTSDTETVHRAIREFLR"
BASE COUNT         1253 a         1428 c         1501 g         1237 t
ORIGIN      
        1 aggcgcgccc ggcgccggtg atgaccacgg cgcggatctc cggatcggct tccgcttggt
       61 ccaatgcgtc gtgaaattct ttcagcattg gccgcgtgat ggcgttgagc gcctcgggcc
      121 gattcaacgt gattagcacg ccgccgcgct gtttttgata aatgatttct ttgtagtcgg
      181 ccattcggcc tcctttgttc aaatcgttca aaccgttcaa gtcgttcaaa tcgtctgttc
      241 actccggtta aacggcttga gctatttgaa cgttttgaac ggcttgaacg agttcctatc
      301 tttattgtta tcggtattca ccttcataca ggttcttgaa gaagccgctg gtttccagtt
      361 cgcggacaat actttcgtcg aggaactgcc tgggatctgc gcccttggct ttgggttctt
      421 cgatggcgac gaaatccaag gtcgtttcga cggccttggg cgtggcatag gggatttggt
      481 tgtaaaactt gttggcaagc agatcgtagg acatttccag atatttggtg tctttttcct
      541 gggccgattg aatgcgcaat ttcttttgca tgacggcgac gctgtcgcgc ttgttctttc
      601 gaaaatacgc catcccttcg caatagcctt tgatgaagcg gacggcttga tcgcggttgc
      661 gtttcaaata gctgcgcgtc gtgtcgatgg agttctgcaa atagacgatg tccaaatcgg
      721 ctggatcggc gagcaaacga tagccgcgct cctcggcaac gaagaaagtc ggcagcgtca
      781 gcactgcgcc gtcgatagcg cggctgtcca gactggcgag gttggccggc gacgagccta
      841 gttggagtat ctgaatatcg tcattgcgca tgttccactt gcggagaaac agttgcagta
      901 cccagtgcga cgctgatccg aagcgggtga cgccgatttt tttgcctttg agatccgctg
      961 gggttttaat ttccgaacgc accattaagc gctgcaagcc ggttttgagc ggcgagaccg
     1021 cgagcacggg atcggcgccc cgcaaggcgg cgcgaattac tgagtcgccg ccgccgacgg
     1081 aaaattgaat ttccccggcg agcagcgaac tgattacccg cgaggcgcca ccgccaatga
     1141 cgataacctc cggatcgacg ccatattttt tgaacaagcc gcgttcttcg gcaacccaca
     1201 tggcgccctg agtggcgctc accgagctca gcccgacgcg caatcgttcc tgtgcgttgg
     1261 ccggctggat cgtccaaagg ccgacaaccg ctacaaaaaa agtggacaaa agtgagtaga
     1321 ccatcggtgt tctatttggc agtgatcgaa acccattgcg ttccggtgaa tccattatcc
     1381 gtcatgccgg cgcacgccgg catccatctt cgcttgtgct gcaagggcaa aaaaaactgg
     1441 attcccgcct gcgccgcaac gacgaaattg gtttccaagc agcactctac ttaactatta
     1501 gaaacgcagg ttagccgtca agggagaggg tgagaaaact attttctgcg cgcgctattt
     1561 ccagacattc tcaagaaagc cggatgattt gatttcgttg acgaaggaat tgtcgtagta
     1621 cgcgctggtc ggcgtcgctt gcgcggcttt cgtatcgatc atgttcagtt gcgccatgcg
     1681 ctgcaccatt gattgcatga cgttctccgg tacggccggg tcgcgggcga aattgtcggt
     1741 tacataagca tagctttggc gcagcacctc ggtgtcggtg atgccgagat attttccgat
     1801 cagtcgcttg gtgaaatctt cgttggcatt gatgaatttc atcgcttcga cagtcgcttt
     1861 tatcaggcgc acaactaaat cccggtgcgt tgatgcgtag gatcgtctgg caacgatgcc
     1921 ctgggatatg aacccacttc ccaaagcgcg caagtctttt ggcgacgcca gttcgcgata
     1981 accttccttg agtaggcgaa aagtatgcgg cggcgagagc acagcgccgt cgatggcgcc
     2041 gcggatgagc gcggcaacgg cttcagggaa gccgcccatt tgtagtgcgc ttacgtcttt
     2101 caagttattc ttttcgagca gagttctgag cgcgaagtcg gtgaccgaac cgaagcgggt
     2161 gatgccgatc ttcttgcctt gcagtgaccg cacatcgcgg atatcgccgc gcaccatcag
     2221 tgactgggtg aagtagggca gcagcgcggc gatctggatg atgtcgccgc cgctcaaaac
     2281 attggtgatc accgccggtc cgccgaaggc cgccaagttc gctgaccctg ataacatcga
     2341 ctgcaccatc acggcggatg cgccgacata aaccatatcg acggtcagtc catttttttc
     2401 gaacagcctc gcctccttgg cgatgacgaa agggacactg ttgagaccga gcgggccgta
     2461 cgggaacggt attttggtcg gttcttgtgc gtgcacgtgc gcatgcacaa gagctgcgcc
     2521 gaccaggatc gcgatagcca ggttgagctt catgattttt atctctgcat agcgcttctt
     2581 tgcggttgcg gtcaagaaaa tagacgcggt tcttgccaag cgaaaatgct ttgcataatc
     2641 attgctgaga cagatttcag tttctattcg gagacgagtg atgccaaaag ttaaaataaa
     2701 cgacattgaa ctctattacg aagaacatgg cagcggccgg cctatggtat ttctgagcga
     2761 gaccgcctgc gacggggaag tttggaagat caatcaagtc ggcgaatttt ccagggacca
     2821 ccgagtgatc acgttcgatt atcgcggcac cgggcagtcg agcaaaccgt cgatcgatta
     2881 cacaacacaa atgttttgcg aggatgtcat cggcttgatg gattatctca aggccgacca
     2941 agcagtcgtc gtcggccatt ccatgggcgg gcgggtggcg caactgatgg cgctcgatca
     3001 tcccaaaaag gtgcacaagc tggttcttgc ttccaccggc gcccactatc cccaaaccaa
     3061 gggcttgccg ctcaagatca tgaaagaaat gatcgagtgg ggctacgaaa aatacgagcg
     3121 cgaccacacg attctcgtcg gctggaccga taattacgtt aagcagcacc cggagaagat
     3181 cgaagcctac ttgaaagtgc gcatgactaa tttatgcccc gttgaatttt atctgcgcca
     3241 tttgatcgcg cgccagagcc acgacacgag ccagcggctg aaagatatca agcagccgac
     3301 gctgatcctg gtcggtgacg gcgacgtcaa catgaccagc gagatgaacc accgcatgtc
     3361 gacggacgtg ttggccaggg ggattccgaa ttctaaagta gtattgctgc caaacgaacg
     3421 gcatagttat tttttcagca atcccgatgc ggcgcaccgc gcgatccgcg agttcatcaa
     3481 ggattgaggg tttgaagcct tggtgggaac cgttaaggcc gaagtgcgtc agcggcgcaa
     3541 ctgaatggga atgaattttc accacgaagt gattcatgag cttatgctcg cctacagatc
     3601 atgaaattgc cctcaccccc gccctctccc agcggactgg gcgagggagt ttccggtccg
     3661 aagaattttt gtctttaccc tcgccctttg ggagagggga gggtgagggc gtcttcaggc
     3721 cagcatcgca tttcaaagga ggacacgaag agcacgaact tcggagaatt aaatctccga
     3781 accttcgtga gcctcggtga gatccagatt gtttgctaaa aggaaccaac ctcatgccga
     3841 ctgtacgcac gaatggcgcc gaaatttatt atgaaactca cggccacggg caaccgtttc
     3901 tatttttcag cgagaccgct tgtgacggcc agatctggaa gatgtatcag gtgccggaat
     3961 tcgccataga tcatcaagta atcgtgcacg actaccgcgg cacggggcgg tcgagcaaac
     4021 catcgatcga ttacacgacg cgcatgttct gcgacgatgc ggtggcgctg ctcgatcacc
     4081 tgaacgccaa agacgccatc gtgctcggcc attccatggg cgggcgcgta gcgcaactgc
     4141 tcgcattgga tcacccacag cgagtaaaaa aattaattct tgcgtcgacc ggagcttcgt
     4201 ttcccggcgg cgccggcttg cccttgcaga tgtgcaagga aatgatcgag tggggctacg
     4261 agaagtacgt gcgcgagcac acgatgcacg tcggctttac cgatgaattc gccaagagcc
     4321 agccggagcg gctcgaagca ttttttcgcc agcgtttgga caatatctgt ccggtggagt
     4381 tttacctgcg acatgtactg tcgcgccagg gacacgatac cagcgcaagg ctcaaagata
     4441 ttgccattcc gacgttggtg atagtcggcg agctggaggg cgatcccaag gttgtcatga
     4501 cgcatcggat gtcatccgat attctcgcca aaggcattcc caatgccaaa ttcgttcttt
     4561 tgccgaagca aaaacataac tacttcacca gcgatacaga aaccgtacat cgcgctattc
     4621 gtgaattctt gcgttaacta ttgcgattgg ctagcgaaaa ccgcgtctat ctacatgcgc
     4681 gcataataac tcgtcgcatg gtaaagagcc ttgagacaga ccgcgctaga tccaccacaa
     4741 tgcgacatct gcaattgggg atctctcggt taaattaatg tagcattttt ggcacagacg
     4801 gtgatcgtcg cgtgccagaa cgggacgtga acggtcgccg gaagagtggt gctcttcaat
     4861 cgttaacaca taactgggct tttcagagaa gacgatctca ttgcgaattt tagcttcgac
     4921 atcgcgcgac aagagactca cgaaatagct gaatacccgg tattaaaccg cttctaatct
     4981 gccgcggttc acgccggcac gtgactttcg ccgcgtcccc ggttgcgcga cggtactaat
     5041 tttgctcgta actacgctag gcggacaaac aatgacgccg aataatacga acaatcgccg
     5101 gcgccgatcg aaaagcgctt ccatcgacaa gaaaaccgag ttccaggccc atgcgacaca
     5161 cgggtcagag catttaagcg actgatggac cgcgtgcagt agcagctgca ggtgtgccgt
     5221 atacgacggc aaagaaattg ccgaaggcag gctaaatgtc ggacgagaaa atgcccatcg
     5281 aacagctcct ccggctactc gcggaacagt cgagggagca cgcgtttatc ctgttgaatg
     5341 cggacggcct gatcacttgg tggagtcccg gcgctgcaca tctattcgat cacgacgccg
     5401 atgagatgat gggccagtc
//
LOCUS       DATLCH010000272         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1458963, whole genome shotgun sequence.
ACCESSION   DATLCH010000272 DATLCH010000000
VERSION     DATLCH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458963"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VMZ02_08010"
     CDS             <1..750
                     /locus_tag="VMZ02_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71916.1"
                     /translation="NWLALSEVKDPKQWWGGHMWVDNAQKFIYMFQAYLTESIWVNTE
                     LAKPGDFRSYDDFLQPKWKGRIGFLDPRTPGAGDSNWAHLWAVKGEEYLKKFVTQDLV
                     LGRDQRVLGENLAKGRVAVMFGLTSYSLLPFINAGLPIKPVSRLKEGTYGTGGSGNVA
                     IIKTPAHPNATKVFINWLLSREGQEIVPRALGQATRRLDVDTKWLRETGVIAAKDQMT
                     VKEFLQIENQSEEKLDKVREPAQKLAGALLK"
     gene            784..2367
                     /locus_tag="VMZ02_08015"
     CDS             784..2367
                     /locus_tag="VMZ02_08015"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR71917.1"
                     /translation="MAGEYFADFRAYLHDLERRGKLHTWTRPVNKDSELMPLMRLQYR
                     GIPDDQRQAFLYENVFDSRGKKFELRVATGVYGSSREIAALGLGCAQPLDIYEKWRNA
                     LAKPIEPRRVATAPVQEIVYTGNELKKFGVTGLPAPVEEPGFSGGIRATAPFITVDPQ
                     TGVRNVGMYSGHFRSDAHLIAGIAPVHHAMLYHHRSAASAKEPLPVAIVLGSLPDVTF
                     VSAANLPYGVDELAVAGGIRGRPVDVVRCKTIPLEVPAEAEIIIEGEISIERKERGEP
                     FSDYPGYLMVEREFRSVIDVKAITMRRNAIFTAILVGLPPSESNGISRTCREMMLYNF
                     LRYSCAMPEVLEVACPEMGGGWNWWVIRMSKSHPSKPKQALHAASGMDPTSKMIIVVD
                     EDIDPKDPDMVMWAMSFAMQPHQDVEISTGRSPLLDPSAHSLMRGREERSYPGAVGCS
                     GLLIDATRKGPFPPVGLPKKSYMENALKIWQEAEMPKLNLKEPWHGYPLGLWDDEDDR
                     LAEKVVQGEYFQHKEVKGH"
     gene            2370..>2995
                     /locus_tag="VMZ02_08020"
     CDS             2370..>2995
                     /locus_tag="VMZ02_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR71918.1"
                     /translation="MAKRDTSYEIWMRQESIPVVEGYGVEDVRQLTLKSWPRTGGKGA
                     FIDLKGMEGFTGMYVGEIPPAGALNPENHLYEELIYIIQGIGATEIWSVGEEKRKMHF
                     EWQQGSLFAIPLNTRHRMINGSKEPVFFLAVTSAPLMIDLLHNISFVMGSDYKFGDRF
                     DGESNYFVPTSSRKEIGGFINWETNFIADARGALVDPSEAKGFGVKITS"
BASE COUNT          686 a          808 c          869 g          632 t
ORIGIN      
        1 aattggcttg cgctctccga ggttaaagat cccaagcaat ggtggggcgg tcatatgtgg
       61 gtcgataacg cgcagaagtt tatttatatg tttcaagcct atttgaccga gagcatttgg
      121 gtcaacacgg aactcgccaa accaggcgat ttccgttcct acgatgattt cttgcagcca
      181 aaatggaagg gccgaatcgg ctttctcgat ccacgtactc ccggagcggg tgattccaat
      241 tgggcgcatc tgtgggccgt gaagggcgag gagtatttga aaaagttcgt tactcaggac
      301 ctcgtcctcg gccgtgatca acgggtgctg ggggaaaacc tcgccaaggg cagggtggcg
      361 gtcatgttcg gacttacctc ctatagcctg ctgcccttta ttaacgccgg tctgccaatc
      421 aagccagtgt cccgattgaa ggaaggaacc tacggtaccg gcggcagcgg caacgtggca
      481 atcatcaaga ctcccgccca tccgaatgcc accaaagttt tcatcaattg gctgttgtcc
      541 cgcgagggcc aagagattgt tccgagagcg cttggacaag cgacgcggcg gctcgatgtc
      601 gataccaaat ggttgcgcga gaccggtgtg atcgcggcaa aagatcaaat gaccgtcaag
      661 gaatttctcc agatcgagaa tcagtccgaa gaaaagctcg acaaggttcg cgagccggcg
      721 cagaagctgg ccggggcact actaaaataa cttcaatcgg cagcggcgaa ggaagtgaat
      781 ggcatggcag gtgagtactt tgcggatttc cgcgcttatc ttcacgatct cgaacgtcgc
      841 ggcaaactgc atacctggac ccgcccggtt aacaaagaca gtgagctgat gccgctgatg
      901 cggcttcaat atcgcggcat tcccgacgat caacggcaag catttttgta cgaaaacgtt
      961 ttcgacagcc gcggcaaaaa gttcgaattg cgcgtcgcta ccggcgttta cggctcgtca
     1021 cgcgaaatcg ctgcgctggg tctcggttgc gctcagccgt tagacatcta cgaaaaatgg
     1081 cgcaacgctt tggcaaaacc gatagaaccg cggcgagtcg ccactgcgcc ggtgcaggaa
     1141 attgtttaca ccggtaacga gctgaagaaa ttcggcgtca ccggtctacc cgcgccggtg
     1201 gaggagccgg ggtttagcgg cggcattcgc gctacagcgc cctttatcac cgttgaccca
     1261 cagacgggtg tgcgcaacgt cggcatgtat agcggccact ttcgcagcga cgctcatctg
     1321 atcgccggca tcgcgccggt gcatcatgcg atgctttatc atcatcgtag cgcggcgagc
     1381 gccaaggagc ccctgccggt ggcgatcgtt ctaggatcat tgcccgatgt cactttcgtt
     1441 tcagccgcaa acttgcccta tggagtcgac gaactcgccg tcgccggcgg catccgcggc
     1501 cggcctgtcg atgttgtacg ctgcaagacg attcccttag aggttcccgc cgaagccgag
     1561 atcatcatcg aaggcgagat ttccatcgag cgcaaggaac gcggcgaacc gttcagcgac
     1621 tatccaggct atctgatggt cgagcgtgaa tttcgctcgg tgatcgacgt taaagcaatc
     1681 accatgcggc gcaatgcgat cttcacggca attctggttg gcttgccgcc gagcgagtcc
     1741 aacggcatct cgcgcacctg ccgcgaaatg atgctctaca attttctccg ttacagctgc
     1801 gcgatgccgg aagttctcga agtagcttgt ccggaaatgg gcggcggctg gaactggtgg
     1861 gtgatccgca tgagcaagag ccatccgagc aagcctaaac aagccctgca tgcggcgtcc
     1921 ggcatggacc cgaccagcaa gatgatcatc gtcgtcgacg aagacatcga cccgaaggat
     1981 ccggatatgg tgatgtgggc gatgagtttt gccatgcagc cgcaccaaga cgtcgaaatc
     2041 agcaccggtc gctcgccgct tctcgatccc tcggcgcact cgctcatgcg cggccgtgag
     2101 gagcggagct atcccggtgc ggtgggctgc tccggtctac tcatcgacgc gacccggaaa
     2161 gggccgtttc cacccgttgg tttgccgaaa aaatcttaca tggagaatgc actgaagatt
     2221 tggcaagagg cggagatgcc taagctcaat ctcaaggagc cttggcacgg ttatccgctg
     2281 ggactttggg atgatgagga cgatcgcctt gctgagaaag tcgtgcaggg cgagtatttt
     2341 caacataagg aagtgaaagg acactgacct tggcaaagcg cgacaccagt tacgaaattt
     2401 ggatgcggca agagagcatt ccggttgtcg aaggttacgg agtcgaagac gtgcgccaat
     2461 tgacgctcaa gtcctggccg cgcaccggcg gcaagggcgc gtttatcgac ttgaaaggta
     2521 tggaaggatt caccggcatg tacgtcggcg aaattccgcc ggcaggcgct ctcaatcccg
     2581 agaatcatct ttacgaagag ctgatttaca tcatccaagg catcggcgcc accgagattt
     2641 ggtcggtcgg ggaagagaaa cgaaaaatgc attttgaatg gcaacaaggc agcttgttcg
     2701 cgattccgct caacacccgc catcggatga tcaatggcag taaagaaccg gtgttttttc
     2761 tcgccgtcac cagcgccccc ttgatgatcg atttgctgca caatatttcg tttgtgatgg
     2821 gcagcgatta caagttcggc gaccgcttcg atggcgaaag caattacttc gttccaacga
     2881 gttcccgcaa agagatcggc ggttttatca actgggaaac caacttcatc gccgatgcgc
     2941 gcggggcgtt ggtcgatcca tcggaagcta aaggttttgg cgtcaagatt acctc
//
LOCUS       DATLCH010000273         8237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1461835, whole genome shotgun sequence.
ACCESSION   DATLCH010000273 DATLCH010000000
VERSION     DATLCH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461835"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1937)
                     /locus_tag="VMZ02_08025"
     CDS             complement(<1..1937)
                     /locus_tag="VMZ02_08025"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71919.1"
                     /translation="MLIRQGAEWPDLFRRQIKNTKKAVADHLKELSAILKQVKSFDDF
                     CAALRQHKQREYLRIGGRDLMPSVPMEETVRELTALADASLEAAYQYCRAEVEKDFGQ
                     LNLPTSGVPNGFVVIGMGKLGGGELNFSSDVDVIFLYENDEGETSGGRKGKTGAREFF
                     NAVGQMIIKAMGDVTEDGFVFRTDLRLRPLGINGPLVQSLDSAMLYYESWGQCWERAA
                     MIKARPVAGAIELGTQFLKELEPFIYRRYLDYTTVDELRHMKERIENELLIGDEKTRN
                     LKLGYGGIREIEFFTQALQLVNGGYEPELRRPSTLPALMELAKRKFITIEERDKLSEA
                     YRFLRQAEHKVQIVQEGHVHSIPDGEAEEQAYARRLGYIHQGKLGESELFWRDHRRYT
                     NTVRNIFDRLFYRAQKDIESEGASVEGSIWNDLDNQQVITKHLAHVGFSDSEKAYENL
                     LAVRDGESYSPPSPKRLKIMRTLGPALIAEIAKSGAPDQALFNLASFSHRIGGRTGFL
                     TLLGENPETMRLLITLFADSQFLTDLFLKRPELIDTLIRVDLTRIDKSKNEMLTELQT
                     SLAALDEMEDKLNALRRYKNEEFIRIGLHDLGGSIDFLGVLRQLSDLAEACLQAALDL
                     ALADLSAKFGEVPNGRFAIIGG"
     gene            complement(2348..2839)
                     /locus_tag="VMZ02_08030"
     CDS             complement(2348..2839)
                     /locus_tag="VMZ02_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HUR71920.1"
                     /translation="MRRARGRLEPLERLEPLKLSSHASRLLNYARTAHLATTDNSGQP
                     HVIPICFVFDRKYFYSPIDEKPKRAAPSKLKRLTNIRENPKVALVVDHYEENWRKLAY
                     VLVFGTARILQSGAKHRSAVKLLRRKYRHYRSMAIHQRPMIAITAKRIVRWGDLESAG
                     LPD"
     gene            complement(2854..3846)
                     /locus_tag="VMZ02_08035"
     CDS             complement(2854..3846)
                     /locus_tag="VMZ02_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71921.1"
                     /translation="MEKLFLTVGCGNYDRTFALRTGDVQAEGIRINYVPLEAEEIFWR
                     MGHHQEFDVSEMSLSNHVTMISRGNSPFVAIPVFPSRYFRHSCVFINTDSGIKSAADF
                     KGKRVGAPEYSITAAVWIRGFLKDDFDVTAADMHWFTGGQEEAGRKERVRLTLPPEIK
                     LDPIADDKTLNGMLENGEIDALISARAPSSFVKGHSKVKRLFPNYKEVEVDYYKRTKI
                     FPIMHVLVIKKEVYDRNPWIARSLYKAFCEAKDLAIKNMHISNTMACTLPWLSWEREQ
                     MREIFGPDWWPYGVEANRHLLDHLIRYMGEQGLLARKVTVEEIFAPNVVGEFKI"
     gene            complement(3947..5704)
                     /locus_tag="VMZ02_08040"
     CDS             complement(3947..5704)
                     /locus_tag="VMZ02_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUR71922.1"
                     /translation="MSTPETTFGTQAPLTSAWTRSRLGVFLSQPVSWVFLMVGGIIAF
                     LSLYPTYFLFYGSLTDAPLGVPGHFTLQNYVKAYGDPEAYSLLLNSFIFGVGASGVSL
                     IFALALAWITIRTDAPWRRLFELTAIIPNILPPILVSISWVMLLNPSNGLINASIVKL
                     FGLEKGPFNIYSMPGLIFVEALILTPLAFLIIAAAMKSMDPSLEESAKTLGSGEFGVM
                     RRITFPLIRPAILAAGTLNFVRAIESFDTPAIIALPARIEVFTTKIWREALGSFPTNH
                     NLAAAYGVGILAVALIFVYLDRRFTSQVESFSTVTGKGFRPHQIELGPWRYAATGVAL
                     FLLILLVLLPILVLSLVAILPYYHVPTWQTWNNLTLSHFQYVWATPRVHKALINSLIL
                     ALLGATLCMLLASLAAYITVRTKIAGRGLIEGLVFIPWAFPGTAMALGLLWAYVDFPI
                     PVYATIWIILIAYVTRFLPYGLRAVTSTIIQVHKELEEASIVCGAGFLATFRRVLIPM
                     MRPGVMAGWIILVTIFMREFSATLFLYSPGAEPLGPLLYFLYLDGMRGRVAAIGLVIS
                     LISVILIAIAQRYSRWDTQ"
     gene            complement(5841..6851)
                     /locus_tag="VMZ02_08045"
     CDS             complement(5841..6851)
                     /locus_tag="VMZ02_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71923.1"
                     /translation="MQPSVHILLIALLSWSLTATAVRAQSGQLLESAKKEGKAVIYGS
                     LEADTMEAVLQGFKKKTGIEAEYWRASATKVMDRAMTEFRAGKAAADAIVAADNVMRL
                     MQKDGFFQKYDSPTNSDFPKDVLDPNLGPRYRNVVVGVVYNKSALKQNEYPKSLDDLA
                     KPQYRGKIVMPDPTQHTTTTQWLASLYKIMGKEKAEKFMRDLAANKPVWVESLLPAAE
                     RVASGEIPIAITYVKYAYIDAKKGAPVDYVRDPKLIGDASYITLASKAPHVNAGKAFI
                     DYFLGDESMNLMSKLGEFVNRKGVLPPLPGADKLGFVEMDDLSTKEFAEKKKEYTQIF
                     LR"
     gene            7031..>8237
                     /locus_tag="VMZ02_08050"
     CDS             7031..>8237
                     /locus_tag="VMZ02_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HUR71924.1"
                     /translation="MAKLDLLIKNGEVWTPGGFIDADIAVDDGKIIALGKSPVLPNSA
                     DQVIDAKGKKIVPGLIDTHTHHHDPGFTYKEDITTATMAAAAGGVTTSIGMPNVNPPT
                     TTVERYRALVEHHSKNAIVDFNHNPSGTVPEEIPGLAKEGPLAFKIFMVKDTGRDYPH
                     MPGIGINNNGELFKCFEAVGSTGLPLMVHPHDQDLMDEIEGRYWKKEDRSPQAYAKAY
                     RDYDGIIWDTAMATLFRMQKALGTPLHILHMTTPEGIAMLRRAKDEGRSCSCEVNPWA
                     MFLGTWENVEKMGPYCLGFWVPPAHVNGLWEAINDGTIDMIGTDHAPHTKEEKDRGWS
                     DMWSCPGGEPQIQDYLSLFLDAVNQGKLSLDRLVRISSFNPAKVFNIYPMKGVLMPGS
                     DADMVIVDMN"
BASE COUNT         1902 a         2166 c         2287 g         1882 t
ORIGIN      
        1 ccaccgatga ttgcgaaccg cccgttggga acttcgccga atttagcgct caaatcagcc
       61 aaggcgagat cgagggcggc ttgcaaacag gcttcagcga gatcggacag ttggcgcagt
      121 acaccgagaa agtcgatcga cccgccgaga tcgtgcagac cgatgcggat aaattcctcg
      181 ttcttatagc ggcgcaacgc gttgagcttg tcctccattt cgtctaatgc ggcaaggctg
      241 gtctgcagct cagtgagcat ctcgttcttc gatttgtcga tgcgtgtcag atcgacacga
      301 atcaatgtgt cgatgagctc ggggcgtttt agaaacaaat cagtcaaaaa ttggctatcg
      361 gcgaatagcg tgattaacag cctcatggtt tccgggtttt cccctaacag cgtcagaaat
      421 ccggtgcgcc cgccgatgcg atgactaaaa ctagcgagat tgaatagcgc ctggtcgggc
      481 gcccccgact tagcgatctc ggctatcaac gccggtccga gggttcgcat gattttcaag
      541 cgttttggac tcggcggcga atagctttcg ccgtctcgca cggcgagcag attctcataa
      601 gccttttctg aatcgctgaa tccgacgtgg gccaagtgct tggtgataac ttgctggttg
      661 tcgagatcgt tccagattga cccctcgaca ctggcgcctt cgctctcgat gtccttttgc
      721 gcccggtaaa agagccgatc gaaaatattg cgtacggtat tggtgtagcg gcgatgatcg
      781 cgccaaaata gctcactttc accgagcttt ccttggtgga tataaccgag ccggcgggcg
      841 taggcttgct cctctgcttc gccgtccggg atcgagtgga cgtggccttc ctggacgatt
      901 tgtactttgt gttcagcttg acgcaaaaaa cgataggctt ctgacagttt gtcgcgctct
      961 tcgattgtaa taaactttcg ctttgccagt tccatcagtg ccggcagcgt actcggtcgg
     1021 cgcaattcgg gctcgtagcc gccgttgacc agttgcagcg cttgagtgaa aaattcgatt
     1081 tcgcgaattc ccccgtaacc gagtttcaaa ttgcgtgttt tttcgtcgcc gatgagcagt
     1141 tcgttctcaa tgcgttcctt catgtggcgc agctcgtcga cagtcgtata gtcaagatag
     1201 cggcgataga taaacggctc gagctctttt aaaaactgtg tgccaagttc aattgcgccg
     1261 gccacaggcc gtgctttgat catcgcggca cgttcccaac attgtcccca agactcgtaa
     1321 tacaacatcg ccgaatcgag cgactgcacc aaaggaccgt tgatgccgag cggacgcagg
     1381 cgcagatcgg tgcgaaagac gaaaccgtct tcggtgacat cgcccatcgc tttgatgatc
     1441 atttgcccga cggcattgaa gaattcccgt gctccagtct tgcctttgcg cccgccgcta
     1501 gtttcccctt cgtcgttctc gtagagaaat attacatcga catcggaact gaagttgagc
     1561 tcgccgccgc ccaacttgcc catcccgatg acgacaaaac cattgggaac acccgaagtc
     1621 ggcaagttga gttggccaaa atctttctca acttccgccc ggcaatactg gtaagcggct
     1681 tccagcgacg cgtcggctaa tgccgtcaac tcgcgcacgg tctcttccat ggggaccgac
     1741 ggcattagat cgcgtccgcc gatgcgcagg tactcgcgtt gtttatgttg gcgcagagcg
     1801 gcgcagaaat cgtcgaagga ttttacttgt tttaagattg ccgagagttc cttgaggtga
     1861 tcggcgaccg ctttcttcgt gttcttaatt tggcggcgaa acaggtccgg ccactccgcg
     1921 ccttgacgaa tgagcacctc gctcaaaaac gaacttcccc ccaatacgcc aaataagctc
     1981 gccaactcgc ctggagaaaa ttgttcgagc gcctttgcgc cgctcgattg aacgagccgg
     2041 gagaaattgg tttcagccag ggtaggggat gggcaggccg ccagatatcg ctgatatcgt
     2101 cggcgtcgct cggaaatctt gcttgtctcc gcccgcgtac tcactagatt acagtaacca
     2161 tctcattact gtccttcaag gctgagtacg agttgggttc gggaagttgg atttcgctta
     2221 aaacgatgca gccatatcga tctgcaggac gtgaattgcg ccgcggcaaa tcgtgggaaa
     2281 ctctccgcaa acatggaagc ggacggtcag catattcaga aattctcgtc ttgcaaccgg
     2341 agagcgttca gtccggcaat cctgcggact ctagatcacc ccagcgaacg atgcgcttag
     2401 ccgtgatcgc aatcattggc cgctggtgga tcgccatgga tcggtaatga cgatattttc
     2461 gccgcagaag tttaacggcg ctgcggtgct tcgcgccgct ttgtagaatc cgcgccgtac
     2521 cgaacacgag aacgtaggcg agcttgcgcc agttttcttc ataatggtcg acgaccaggg
     2581 caaccttggg gttctcccgg atattcgtca gccgtttcag cttgcttgga gcggcgcgtt
     2641 tcggcttctc atcgatgggc gaatagaaat atttcctgtc aaagacaaaa cagatcggga
     2701 tcacatgagg ttggccagag ttatcggtag tcgcgagatg agcagtgcgt gcgtaattga
     2761 gtaggcgcga ggcgtgcgag gacagtttca ggggttccaa tcgttccaac ggttccagcc
     2821 gtccacgcgc gcgtctcacg cctcgtgcct gctttaaatc ttaaactctc ccactacgtt
     2881 gggcgcgaag atttcttcga ccgtgacttt gcgcgccaac aggccctgct cgcccatgta
     2941 acgaattaag tggtcgagta ggtggcggtt ggcttcgacg ccgtagggcc accagtcggg
     3001 accgaagatt tcgcgcatct gttcgcgttc ccaggagagc cagggcagcg tgcacgccat
     3061 ggtgttggag atatgcatat ttttgattgc caggtctttg gcttcgcaaa atgccttata
     3121 caggctgcgg gcgatccatg gatttctatc gtatacttct ttcttgatca ccagcacatg
     3181 cataatcgga aatatcttgg tgcgcttgta gtagtcgacc tcgacttctt tatagttcgg
     3241 gaacaaacgt tttaccttgg agtggccttt gacgaaggat gacggcgccc gcgcggaaat
     3301 caatgcatcg atttcgccgt tttccaacat tccatttaaa gttttgtcgt cggcgatcgg
     3361 atcaagtttg atttccggcg gcaacgtgag tctcacccgc tcttttctgc cggcctcttc
     3421 ttgaccgccg gtgaaccagt gcatgtcggc agccgtgacg tcgaagtcgt ccttcaaaaa
     3481 accgcggatc cacaccgctg cggtaataga atattcgggt gcgcccactc ttttgccttt
     3541 aaaatcagcc gcgctcttga tgcccgagtc ggtgttgatg aatacgcagg aatggcggaa
     3601 ataacgcgac ggaaagaccg gaatggcgac gaagggcgaa ttgccgcgac tgatcatggt
     3661 gacatgatta gacagcgaca tttcggagac atcgaattcc tggtgatggc ccatgcgcca
     3721 aaagatctct tcggcttcga gcggtacgta gttgattcgg atgccttcgg cctggacgtc
     3781 gccggtgcgc agcgcaaagg tgcgatcgta gttgccgcag cccactgtta gaaataattt
     3841 ttccactctt gttcccccta ttttgtgtcg agtcggagat ggaagatcga ggatggagga
     3901 ggccatcgat tcctcaactc tcaatcatcc attctcaatt atcaacttac tgggtatccc
     3961 agcgcgagta acgttgcgcg atggcaatta agatcactga aatgagcgag atcaccaggc
     4021 cgatggccgc gacacggccg cgcatgccgt cgagatagag aaaataaagc agtggtccca
     4081 gcggttcggc gccgggactg tataaaaaca aggtcgcgct gaactcgcgc atgaaaatcg
     4141 tcacgagaat aatccagccg gccatgacgc cgggccgcat catcggaatc aggacgcggc
     4201 gaaaagttgc caggaaaccg gcgccgcaga caatcgaagc ttcttcgagt tctttgtgta
     4261 cttggatgat ggtactggtg acggcgcgca gtccgtaagg tagaaaacgc gtaacgtagg
     4321 cgatcagaat gatccatata gtggcgtaaa ccggaatcgg aaaatcgaca taagcccaga
     4381 gcagccccaa tgccatcgcc gtgccgggaa acgcccaagg aatgaaaacc aaaccttcga
     4441 tcaggccgcg accggcaatt ttcgttctga ccgtaatgta agcggccaac gaagctagca
     4501 gcatgcacag agtcgcgccg agcagggcga gaatgagact attgatgagt gccttgtgga
     4561 cgcgtggcgt ggcccagaca tattgaaaat gtgacagcgt taagttgttc cacgtctgcc
     4621 aagtcggaac atgatagtac ggcagtatgg caacgagcga taataccaat atcggtaaca
     4681 gcaccagcaa aatcaataag aacagggcga cgccagtcgc ggcgtaacgc caaggaccga
     4741 gctcgatctg gtgcggccga aatcctttac cggtgacagt ggaaaacgat tccacttggg
     4801 aagtaaagcg gcggtcgaga tagacgaaaa tgagcgcgac ggcaagaatg ccgacgccgt
     4861 aggcggcggc aaggttgtga ttggtcggaa acgagcccaa cgcctcgcgc cagatcttcg
     4921 tggtgaacac ttcgatgcgc gctggcagcg cgatgatcgc cggcgtgtcg aagctttcga
     4981 tggcgcggac gaaattcagg gttccggcgg cgagtatggc cggtcgtatc aatgggaaag
     5041 taattcggcg catcacaccg aattcgcccg atccaagagt cttggccgac tcttccaatg
     5101 acggatccat gcttttcatc gccgcggcga tgatcagaaa cgccagcggc gttaagatca
     5161 gcgcttccac aaaaatcagt ccaggcatcg aataaatgtt gaacggccct ttctccaggc
     5221 cgaagagctt tacgatcgaa gcattgatca aaccgttgct aggattgagc agcatcaccc
     5281 aagagatcga aaccaggatc ggcggcaaaa tattcggaat gatcgcggtg agctcgaaca
     5341 gccgacgcca cggcgcgtcg gtgcggatgg ttatccaggc cagtgccagg gcaaagataa
     5401 gcgacacccc ggacgcaccg acgccgaaga taaatgagtt caacaacagc gaatacgctt
     5461 cgggatcgcc gtaagccttg acgtagtttt gcaatgtgaa gtgaccgggc actcccaacg
     5521 gcgcatcggt gagactgccg taaaatagaa aatacgtcgg gtagagactc agaaaggcga
     5581 tgataccgcc caccataaga aacacccagc tgacgggttg gctgagaaag acaccgaggc
     5641 gcgaccgggt ccatgctgag gtgagcggcg cttgggtgcc gaaagtggtt tcgggggtgg
     5701 acacgaatgg atccagctag cagattactg aaaattctat cggattcctt tgacgggctc
     5761 aggaccaacg agaagtaatt atactagttc cgttcatgct gagctcatcg aagcagcttt
     5821 tggcgtattt ctggagctcg ctaacgcaag aaaatttgtg tgtattcctt tttcttctcg
     5881 gcaaattcct tagtgctcaa gtcgtccatt tcgacgaagc ccaacttgtc tgcgccaggc
     5941 aagggcggca gaactccctt gcggttcaca aactcgccca acttcgacat caagttcata
     6001 ctctcgtcgc cgagaaaata gtcgatgaag gctttgccgg cgtttacatg cggtgctttg
     6061 ctagcgagtg tgatgtagct ggcatcgcca atgagtttgg gatctctgac ataatccacc
     6121 ggcgcgccct tcttagcatc tatgtatgca tatttgacgt aggtgatagc gatgggtatt
     6181 tcaccgctgg caacccgctc tgctgcgggc aaaagcgatt cgacccatac cggtttgttg
     6241 gcggcgagat cgcgcatgaa tttctccgcc ttctccttac ccatgatttt gtacaaactc
     6301 gccagccatt gtgtcgtagt cgtgtgctgg gtcgggtccg gcatgacaat cttgcctctg
     6361 tattgcggct tggcgagatc atcgagcgat ttcgggtatt cgttctgctt cagcgccgat
     6421 ttgttataga cgacgccgac gacgacattc cgatagcgtg gtcctaggtt tggatcgaga
     6481 acgtctttag gaaaatcgct attggtagga gaatcgtatt tctgaaaaaa accgtctttt
     6541 tgcatcaggc gcatgacgtt gtcggcggcg acgatggcgt cggcagccgc tttgccggcg
     6601 cggaactcgg tcatggcgcg gtccatcacc ttagtcgccg aagcacgcca gtactccgcc
     6661 tctatgccag ttttcttttt gaatccttgc aggacggcct ccatggtgtc ggcttcgagt
     6721 gatccgtaga tgaccgcttt accttctttt ttcgcggatt cgagtaactg gccactctgc
     6781 gcgcgcactg ccgtcgccgt taacgaccaa ctgagtaagg cgatgagcaa aatatgaacg
     6841 gaaggttgca tggatttctc cttggttgga cgctcgaggg tcggcgaagc gcagcgatat
     6901 tttcagattt caccgagtga gtcaagtaaa ctttggcgag gctgacgatc ttctccactc
     6961 gtagattgac tccgttaagc gcggatcata taaaagattt tttgcagcgg cgaagggaga
     7021 atggaaattc atggctaagt tggatttgct aattaaaaac ggcgaggtgt ggacgccggg
     7081 cggttttatc gacgccgata ttgcggtcga cgacggcaag atcattgctc tcggcaagtc
     7141 gccggtgttg ccgaacagcg ccgatcaggt gattgacgcc aagggtaaaa aaattgtccc
     7201 cggtttgatc gatactcaca cgcatcatca cgacccaggc ttcacttaca aagaagacat
     7261 cacgaccgcg accatggcgg cggctgccgg tggtgtgacc acgtcgatcg gcatgcccaa
     7321 tgtgaaccca ccgactacga cagtggagcg ttatcgcgca ctagtcgaac atcacagcaa
     7381 aaacgctatt gtcgacttca atcacaaccc ctccggcacg gtgccggaag agattcccgg
     7441 tctggctaag gaagggccgc tagcctttaa aatttttatg gtcaaggata ccggccgcga
     7501 ttatccgcat atgccgggca tcggcatcaa caacaacggc gaattgttca aatgctttga
     7561 agccgtcggc agcaccggac tgcctttaat ggtccatccg cacgatcaag acttgatgga
     7621 cgagatcgaa gggcgctact ggaagaaaga agaccgcagc ccgcaggcct acgccaaagc
     7681 ctaccgcgat tacgacggta tcatttggga cacggcgatg gcgacactgt ttcgaatgca
     7741 gaaagcgctc ggcacgccgc tgcatatttt gcacatgacc acgccggaag gcatcgccat
     7801 gctgcgccgc gccaaagatg aaggccggtc ctgtagctgc gaggtcaatc cttgggcgat
     7861 gttcttgggc acttgggaga atgtcgagaa gatgggcccc tactgcttag gtttttgggt
     7921 gccgccggcc catgtgaacg gcttgtggga agcaatcaac gacggcacca tcgacatgat
     7981 tggcacagac cacgcaccgc ataccaaaga agaaaaagat cgcggctggt ccgatatgtg
     8041 gagttgtccc ggcggcgagc cgcagatcca ggattatctg agtctatttc tcgacgcggt
     8101 gaatcaaggc aagctgtcgc tcgatcgctt ggtgcgcatc tcgtcgttca atccggccaa
     8161 ggttttcaac atctacccga tgaagggcgt gctcatgccg ggctccgatg ccgatatggt
     8221 gatcgtcgat atgaata
//
LOCUS       DATLCH010000274         3391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2956913, whole genome shotgun sequence.
ACCESSION   DATLCH010000274 DATLCH010000000
VERSION     DATLCH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2956913"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..899
                     /locus_tag="VMZ02_08055"
     CDS             48..899
                     /locus_tag="VMZ02_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71925.1"
                     /translation="MAQIVSIIGITHNPFMPRLFKQAQQPPGAAKVKERIAMMRQKLA
                     EAKVDVLVCIGNDHLHQFFMDNMPAFMIGKMEQYDGTFYDEEREFGLPKCKLQGDLDV
                     SDAIMEGAFDRGVDFSYSNELTIDHSIIVPMLFVRPEMDIPIVPILTNCIAPPMPRPK
                     RYFEVGKALRAAIDSLPTNKRIGVLVSGHLSLEIGGPKMFEPKFTDPNFDANAVGWIV
                     NGNIDAAAEACAPEKVIQYGNMTTGYLNFIMMMGLANLTKPSYAEALDAGFPAIPFFS
                     WDQQAQA"
     gene            918..1190
                     /locus_tag="VMZ02_08060"
     CDS             918..1190
                     /locus_tag="VMZ02_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HUR71926.1"
                     /translation="MDFKILSTVFTSVFIAELGDKTQLATILFASDKDTSKLTVFVGA
                     AAALIVTSAIGVGAGSIVSQYVSEKTLQYVAGIGFIAIGIWTLVKA"
     gene            1204..1515
                     /locus_tag="VMZ02_08065"
     CDS             1204..1515
                     /locus_tag="VMZ02_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71927.1"
                     /translation="MSKYLMNKLIHLVNMDEKAEEKYCANPRAFVEEWEKTQKLKLTP
                     DEREALATKDYGKLYALGAHPFTLWSFTEAVWIHEKPRPDLVADYKAKAAKTGYPNFK
                     T"
     gene            complement(1557..2858)
                     /locus_tag="VMZ02_08070"
     CDS             complement(1557..2858)
                     /locus_tag="VMZ02_08070"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR71928.1"
                     /translation="MITSEENQLLTQTNPDKPAGELLRRYWQPVALCTELPMDGAPLC
                     VKILGETLALFRDDHGRIGLLGLHCSHRGTDLSYGRIEDGGLRCLYHGWLYDIHGRCL
                     EQPGEPGGGANRDAIHHRAYPCQEKGGVIFTYMGPGKAPLLPNYEFLNQPPGHFCVYK
                     VYHECNYLQANEGNIDPVHLSFLHKFLEHKEERYTGVRGSEESHYNLIAKAQPPALEV
                     EVTDFGVRIYSVRQMGEGKIYLRVSNFVLPNLSTFPGQTGGQGYGINWHVPIDYTHHW
                     KYCFFFNREAPLDQAVIDKSLAEVDEHYRPVRNEANRFMQDRQSMQTQTYTGMGSGFQ
                     THDAFATGSQGAVQDRTQEHVVSSDIAIVAARKLMEKAIRDIQEGREPPHVIRTAEQN
                     SLSHLLVISDLVSGVSDWKGYTRAMEQEARDRHEARGKRRE"
     gene            complement(2883..>3391)
                     /locus_tag="VMZ02_08075"
     CDS             complement(2883..>3391)
                     /locus_tag="VMZ02_08075"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71929.1"
                     /translation="SFLWKTKGEEYLTKLAQQDLLVNQNLRQLADGLAKGKLAFTLGL
                     SHYTYEPFIKAGLPVKPVTRVREGAHANNGSGVVTVIKNPPHPNAAKIFVNWLLGKEG
                     QELYGKAMTQGTRRLDVNTAWLKASGSEGCKDVMSVDDYLRLETHLESSVKKIRAPAI
                     TLAAKLLK"
BASE COUNT          770 a          969 c          879 g          773 t
ORIGIN      
        1 taagccatca tgctaaacag caaatgatcc gtcggtagga ggaaattatg gcccagatcg
       61 tgtcgattat cggcattacg cataatccat tcatgccccg cttgttcaaa caagctcagc
      121 agccgcccgg cgccgccaaa gtcaaagaac gcatcgccat gatgcgccag aaactcgccg
      181 aagccaaagt cgatgtgctc gtctgcatcg gcaatgatca cttgcatcag ttttttatgg
      241 acaacatgcc ggcattcatg atcggcaaga tggagcagta cgacggaacg ttctacgatg
      301 aagagcgcga attcggcttg cccaaatgca aactccaggg cgatctcgat gtttccgacg
      361 ccattatgga gggggccttt gatcgcggcg tcgacttttc ttattcaaac gaactgacca
      421 tcgaccactc gatcatcgtg cccatgttgt tcgtccggcc tgagatggat attcccatcg
      481 tgccgatttt gaccaactgc atcgccccgc cgatgccgcg cccgaaacgc tacttcgagg
      541 tcggtaaagc gctgcgcgct gccattgaca gcctgccgac aaacaagaga atcggcgtcc
      601 tcgtcagtgg ccatctgtcg ctcgaaattg gcgggccgaa aatgttcgag cccaagttta
      661 cagatccaaa tttcgacgcc aatgctgtcg gttggattgt taacggcaac atcgacgccg
      721 cagccgaagc ctgcgcgccc gagaaagtta ttcaatacgg caacatgacc actggctatc
      781 tgaatttcat catgatgatg ggcctcgcca atttgaccaa accgtcctac gccgaagcac
      841 tcgacgcagg ttttccggca atcccgttct ttagctggga tcaacaggct caggcttgat
      901 ggtcgaaaga cttactaatg gattttaaaa ttctttccac cgtgttcact tccgtcttca
      961 tcgccgagct cggcgacaaa actcagcttg cgacgatatt attcgcgtcc gacaaggaca
     1021 ctagtaaact gaccgtcttt gtcggcgccg ccgcagctct catcgtaacc tctgccatcg
     1081 gcgttggcgc aggctcgatc gtttcgcagt acgtcagcga gaagactttg cagtacgttg
     1141 ccggaatcgg ctttatcgca atcggcatct ggacgttggt taaagcgtag ccaaggagcg
     1201 atcatgagca aatacctaat gaacaaactg attcacctcg tcaacatgga tgagaaagcc
     1261 gaagaaaagt attgcgccaa cccgcgcgcg ttcgtcgaag aatgggagaa aactcagaag
     1321 ttaaaactga cgccggatga acgtgaagcg ctcgcgacca aggactacgg caaactttac
     1381 gcgctgggcg cccatccgtt cacattgtgg agctttaccg aggcggtctg gatacatgaa
     1441 aagccgcgac ccgatttggt ggcggactac aaagccaaag ctgccaagac agggtatccg
     1501 aactttaaaa cctgagttag gcgcgagcca cgaggcttga ggcatgagat cctatcttac
     1561 tcgcgccttt tgcctcgcgc ctcatgccta tcgcgcgctt cctgctccat cgcccgcgtg
     1621 taacccttcc aatcgctgac gccgctcacc aaatctgaaa tcaccaacag gtgagataaa
     1681 ctattctgct ccgccgtgcg aatcacatgg ggtggttcgc gaccttcttg aatgtcgcgg
     1741 atggcttttt ccatgagctt gcgcgccgcc acaatggcga tatcggacga cacgacatgc
     1801 tcctgtgtgc ggtcttgcac tgcgccctgg ctaccggtag cgaaagcgtc gtgggtctga
     1861 aaaccgctcc ccatgccggt ataagtctgc gtctgcatag attggcggtc ctgcataaaa
     1921 cgattcgcct cgttgcgcac cggccggtag tgttcgtcaa cttccgcgag tgacttgtcg
     1981 atcaccgctt gatcgagcgg ggcttcgcga ttgaagaaga agcaatattt ccagtgatgg
     2041 gtgtagtcga tcggcacgtg ccagttgatg ccatagcctt gcccgccggt ctgacccgga
     2101 aacgtgctca gattcggcaa gacaaagttt gaaactcgca ggtaaatctt gccctcgccc
     2161 atctggcgca ccgaataaat acgcacacca aagtccgtga cctcgacctc caatgccggc
     2221 ggctgcgctt tggcgatcaa gttgtaatgt gactcttcgc tgccgcgcac accggtgtat
     2281 cgctcttcct tgtgttcgag aaacttatgt aagaacgaca agtgcaccgg atcgatattg
     2341 ccttcgttgg cctggagata attgcactcg tggtaaactt tgtagacaca aaaatggccc
     2401 ggcggttgat tcaagaattc gtagttgggc aacaaaggcg ctttgcccgg ccccatataa
     2461 gtgaaaatca ccccgccttt ctcctgacac ggataggcac gatggtgaat cgcatcgcgg
     2521 ttggcaccac cacccggctc acctggctgt tccagacaac ggccgtgaat gtcgtagagc
     2581 cagccatgat agagacaccg cagcccgcca tcttcgatgc ggccgtagct caaatccgtg
     2641 ccgcgatggg agcaatgcag gccgagcaaa ccgatccggc cgtgatcgtc gcgaaacaac
     2701 gccagcgtct cgccgagaat tttgacgcat agcggcgcgc cgtccatcgg aagctcggtg
     2761 cataacgcca cgggttgcca gtagcgccgc agcagttctc ctgcgggctt gtcggggttg
     2821 gtttgagtca acagttgatt ttcttcgctg gtaatcatcg cgcaatttca actttcctaa
     2881 tattacttca aaagcttggc cgcgagcgta atagccggag ctctaatttt cttcaccgac
     2941 gattccagat gagtttccag tcggagataa tcgtcgaccg acatgacgtc tttgcaaccc
     3001 tcgctgccgg atgctttcag ccacgccgtg ttcacgtcca gccggcgcgt accctgcgtc
     3061 atcgccttgc cgtagagctc ctgaccttcc ttgccgagca gccagttgac gaagatctta
     3121 gcggcgtttg gatgcggggg attttttatc accgtgacca cgccgctgcc gttgttggcg
     3181 tgggcgcctt ctctgacgcg cgtaaccggc ttcaccggca gcccggcttt gatgaacggt
     3241 tcgtaggtgt agtggctcaa accaagagta aaggcgagtt tgcctttagc caagccgtcg
     3301 gccaattggc gcaagttctg attcaccaac aaatcctgtt gcgccagctt ggttagatac
     3361 tcctcgcctt tggttttcca taaaaacgac c
//
LOCUS       DATLCH010000275         3108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4423713, whole genome shotgun sequence.
ACCESSION   DATLCH010000275 DATLCH010000000
VERSION     DATLCH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4423713"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VMZ02_08080"
     CDS             <1..557
                     /locus_tag="VMZ02_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71930.1"
                     /translation="LRKLNIDPEKDTRIITAGGNPERFAALTKGVINFTIMPEPFVRQ
                     ALQMGFRHLFDIGSLKIPFWWNAVLTREAIVKAKRPLLLKFTRAMIEATHFNKTNKEE
                     AKAIWGKYLRITDSEALERAWQTYTAAYPDNLLPTPDGVKTLLDDIAPREPKAANADA
                     RRFVDPSLVQEVEATGFVKQLYKR"
     gene            complement(694..2013)
                     /locus_tag="VMZ02_08085"
     CDS             complement(694..2013)
                     /locus_tag="VMZ02_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71931.1"
                     /translation="MAYSAQGRTKDFPVFDCDSHIYEPPEIWDKYIPQHNRDFAKTHF
                     YRDADRLFLVRNSRISFRDPDKWKYPGETWHPGLTKKIIGATQPGTKDWDETIGRNRS
                     ARDPHARLKDMDASGIDQVMIFPSTFVYLPLVENAEAAYICARAYNDWAYDYCSADKE
                     RLFPAAVLPTQNVDFAIEELRRVAKRGCKTALVRPIIAQGKYPTFPEYDPLWREFEEL
                     GVVLGMHTFPSRGEAMSAELDKRMGANRKRLFSDEDILVYSPGQFVANIMQIMGSKQA
                     GDAAFGFMAEAMTWTGVVLMTGWLEKFPRLKVAILESNSSWLPLVLEKAETYLDLYKH
                     IGEKIGDPHEVFYKQCFIAFESDEMITLRLWDLFENIALWSSDMPHLDASDVWEAIDQ
                     MDRYKVPQSARVKMLGGNARRLYGIEPKLVLTKAPDEYRPQTMSRYA"
     gene            complement(2271..2483)
                     /locus_tag="VMZ02_08090"
     CDS             complement(2271..2483)
                     /locus_tag="VMZ02_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71932.1"
                     /translation="MKDITVPTLIFVGDDDHFVVSDMSHRSGADVLAKGIPNAKLIVL
                     PGARHSYFYTEPEQVHGMMREFLREN"
     gene            complement(2480..>3108)
                     /locus_tag="VMZ02_08095"
     CDS             complement(2480..>3108)
                     /locus_tag="VMZ02_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71933.1"
                     /translation="WLPERRRSMPYVKARDGTNIYYEATGKGKPFLFLSETACDGAVW
                     NLYQTPEFSSDHCVVTLDYRGTGLSGKPSERYSTAMFAEDAVAVLDHLGLDGAVVCGH
                     SMGGRVAQLLALDHPRKVSKLILASSGAAHPGDRGIPLRIAKEMAEWGYVKYVRDHTI
                     VVGWTEEYARNHINDIENISRCAWPTCARWSFTSAMCWRARSMTAVDG"
BASE COUNT          706 a          902 c          825 g          675 t
ORIGIN      
        1 ccctgcgcaa gttgaacatc gacccggaaa aagacacccg gatcatcacc gccggcggca
       61 atccggaaag gttcgcggcg ctgaccaaag gagtgatcaa ttttacgatc atgcccgagc
      121 cgtttgtccg gcaagcccta cagatgggct ttcgtcacct gttcgacata ggctcgttga
      181 aaataccgtt ctggtggaat gccgtgttga cgcgggaagc gattgtcaaa gccaaacggc
      241 cgctgctgtt gaaattcacc cgggcaatga ttgaagcaac tcacttcaac aagaccaaca
      301 aggaagaagc caaagcgatt tggggcaagt acctgcgcat caccgactca gaagctctcg
      361 aacgcgcttg gcaaacctat accgcagcct accccgacaa cctgctgccg acgcccgatg
      421 gcgtaaagac cctgctcgac gacatcgcgc cgcgcgaacc gaaagccgcg aatgccgatg
      481 cacgccgctt tgtcgaccca tccttggtgc aagaagtcga agccaccgga ttcgttaaac
      541 agctctataa aagatagggc gcagaaccgg gcctattaga ggttcaaata gttcaaaacg
      601 ttcaaacagt tcaatagttg tcggacagga acgttttgcg actcctgtcc cccgttttga
      661 acgagttgaa cgttttgaac gcgttaaatt cacctacgcg taccgcgaca tcgtctgcgg
      721 ccggtactca tccggcgcct tggtaagaac tagcttcggc tcgataccgt agagccggcg
      781 ggcgttgccg ccgagcattt tcacgcgggc gctttgcggc actttgtatc tgtccatctg
      841 gtcgattgct tcccagacat ccgaagcatc gagatgaggc atatcggacg accacagggc
      901 gatgttctcg aacagatccc ataaccgcag cgtgatcatt tcgtcgcttt cgaaagcaat
      961 gaagcattgt ttataaaaaa cctcgtgcgg atcgccgatc ttttcgccga tgtgcttgta
     1021 gagatcgagg tacgtctcgg ctttttccaa caccagcggc agccagctgg agttggattc
     1081 cagtatcgcc actttgaggc ggggaaattt ttccaaccag ccggtcatca atacgacacc
     1141 ggtccaggtc atcgcctcgg ccataaaacc aaaagcggca tcgcctgcct gcttggaccc
     1201 catgatctgc atgatgttcg cgacgaactg ccccggtgag tagactaaaa tgtcttcgtc
     1261 ggagaatagc cgcttgcgat tggcacccat gcgcttgtcg agttccgctg acatcgcctc
     1321 accgcgcgag ggaaacgtgt gcatacccaa cacaacgccg agttcctcga actcgcgcca
     1381 tagcgggtcg tattccggaa acgtcggata cttaccctgg gcgatgatcg gccgcaccaa
     1441 tgcggttttg cagccgcgtt tggcgactcg gcgcaactct tcgatggcga aatcgacatt
     1501 ctgcgttggc aatacggcgg cgggaaaaag ccgctccttg tcggcgctgc agtagtcgta
     1561 agcccaatcg ttataggcgc gcgcgcagat gtaagccgcc tcggcgtttt ccaccagcgg
     1621 caggtaaacg aatgtcgatg ggaaaatcat tacctgatcg ataccggaag catccatatc
     1681 tttgagccgc gcatgaggat cgcgggcgct tcggttccgg ccaatcgtct cgtcccagtc
     1741 tttagtgccg ggctgagtcg ctccgatgat cttcttcgtc aagcccggat gccaagtctc
     1801 accgggatat ttccacttat cgggatcgcg aaagctgatc cgtgagttac gcaccaaaaa
     1861 caaccgatcc gcgtcccgat aaaaatgcgt cttggcgaaa tcgcggttat gctgcggaat
     1921 atacttatcc caaatctccg gtggctcgta aatatggcta tcgcaatcga acaccggaaa
     1981 gtctttggtt ctaccctgag cgctgtaagc catactcccc tccgttatct ttgttctctc
     2041 ctgcgtaaac ttcgtgtcgt acttaagaac atacatgcgc aagtgcgatc ggggcgacga
     2101 attgccataa cgattcgctc ataccggtga aggccgatat ctatcccctt cctcgcctgg
     2161 atgccggcct tcgccggcat gacgaagcgg ggaacgggcg tgcaggggat tgcaattcac
     2221 actctgtaga caagcgtaag ttattggcat ttcgcggtga ccgctatttc tcaattctcg
     2281 cgcagaaact cgcgcatcat gccgtgcacc tgttccggtt cggtgtagaa atagctgtgc
     2341 cgcgccccgg gcaatacgat caacttggcg ttggggattc ccttagccaa gacatcggcg
     2401 ccgctgcggt gcgacatatc gctgacgacg aagtggtcgt cgtccccgac aaaaatcaga
     2461 gtcggcaccg tgatatcttt caaccgtcca ctgctgtcat gctcctggcg cgccagcaca
     2521 tggcggaagt aaaactccac cgggcacagg ttggccatgc gcacctggag atattttcga
     2581 tgtcgtttat gtgattgcgc gcgtactctt cggtccagcc gacaacgatc gtgtgatcgc
     2641 ggacatattt tacgtaaccc cattcggcca tttccttggc gatgcgcaaa ggaatgccgc
     2701 gatcgcccgg atgagctgcg ccgctggacg cgagaatcaa cttgcttacc ttgcgcggat
     2761 gatcgagggc gagcagttga gccacccgcc cacccatgga gtgaccgcaa acgacggcgc
     2821 cgtcgagacc gaggtgatcg agcacagcaa cggcatcctc ggcaaacatc gcggtgctat
     2881 atctctcgga cggtttgccg gacaagccgg tgccgcgata atccagcgtc acgacgcaat
     2941 gatccgagga aaactccggg gtctgataaa ggttccacac ggcgccgtca caggcagtct
     3001 cgctcaaaaa taaaaacggc ttccccttgc cggtcgcctc gtagtagata ttcgttccat
     3061 cgcgggcttt cacataaggc atggatcgtc tcctctcggg tagccata
//
LOCUS       DATLCH010000276         7467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10801899, whole genome shotgun sequence.
ACCESSION   DATLCH010000276 DATLCH010000000
VERSION     DATLCH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10801899"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VMZ02_08100"
     CDS             <1..251
                     /locus_tag="VMZ02_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71934.1"
                     /translation="KSLKEIQTLLGDWHDRAVLQKHVEKFIRRPGFLTDRPDSGRILL
                     AELEKERLRNDSEVTRVLQNAGKVQHSWTIWKASGAHE"
     gene            complement(267..623)
                     /locus_tag="VMZ02_08105"
     CDS             complement(267..623)
                     /locus_tag="VMZ02_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71935.1"
                     /translation="MQCLTLARAIVRVMDPEWLREVYGFASDAIFYLRVAIDDLVTRV
                     LQSGGFDYWESGMDLRMGDDLYESFIRYQKWLLAEFDKMVEPNGFEIVDASNSIEEVF
                     ETLRRQVDRVIARNGD"
     gene            831..1820
                     /locus_tag="VMZ02_08110"
     CDS             831..1820
                     /locus_tag="VMZ02_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HUR71936.1"
                     /translation="MKNIFLALCLLTLAGISPRAATAQVIQIDGSSTVFPVTEAVAEE
                     FQKSKKGKVKVTVGIAGTGGGFKKFCRAETDISNASRPILKSEIEDCKKSGVQFIELP
                     VAYDALTVIVNPKNDWVKSLAVADLKKMWEPSAQGKVTTWNQIRPEWPNNPLKLFGPG
                     ADSGTFDYFTEAIVGKAKSSRGDFTASEDDNVLVQGVANDRNALGYFGFAYYIENQSK
                     LKAVAIETGKGAVVPSAKTVEDGTYQPLSRPIFIYVSKKSLDTKPEVKEFVDFYLKNA
                     AKLVKDVKYVALPQSAYTVGAEHVSKGKIGTVFGGTAEVGVKIDDLLKREANF"
     gene            1889..2839
                     /gene="pstC"
                     /locus_tag="VMZ02_08115"
     CDS             1889..2839
                     /gene="pstC"
                     /locus_tag="VMZ02_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HUR71937.1"
                     /translation="MANTEVAVELSAVRHHIRAHSRSMTSQKIRERIIESAMFLCAVF
                     SVAITAGIVWVLVYESWNFFRNVSMIDFLTDTQWTVLFENPRYGIMPLVTGTLVTSFI
                     ALIVALPLGTTIAIYLSEYANPTVREILKPILELLSAVPTVVFGYFALLFVTPMLQRI
                     LPDLPGFNMLSAGIVIGIMIVPYVSSLSEDAMKAVPVTIREGSYAMGATRVQTALRVL
                     MPAAFSGIASAYVLGFSRAVGETMVVAIAAGTQPNLTLNPMEGAATITAYIVQVSLGD
                     LPHGSVAYQSIFAAGLTLMVMTLIFNIGGYFLRKRFREVY"
     gene            2842..3765
                     /gene="pstA"
                     /locus_tag="VMZ02_08120"
     CDS             2842..3765
                     /gene="pstA"
                     /locus_tag="VMZ02_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HUR71938.1"
                     /translation="METPSVAAIEKRVAANKARDQTFAIIGLICTLVGMLTLAALLFD
                     LASDGLARIKPHFFTSFPSRFPGQAGILSSWVGTILVMIVTAMAAVPIGIGAGVYLED
                     YAPKHWLTALIEINIANLAGVPSIVYGLMALGLFVYQFKFGQSIMSAGLTLALLILPI
                     VIVATREAIRAVPGSMREAAYALGATKWQTTSDHVLPYSMGGILTGVIIALSRAIGET
                     APLITIGALTFIAFLPDAPVKAEFPYISFDWLWSPYSVMPIQMFNWVSRPQAEFHLNA
                     AATGLILMIMTLAMNGVAIYFRYRFRKRIKW"
     gene            3877..4659
                     /gene="pstB"
                     /locus_tag="VMZ02_08125"
     CDS             3877..4659
                     /gene="pstB"
                     /locus_tag="VMZ02_08125"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HUR71939.1"
                     /translation="MIRASSETQHKDIFSVENVDFYYGQKRALNSINLAIAEKKATAF
                     IGPSGCGKTTLLRCFNRMNDLVLGTRVTGQIMLDGKDIYEPTTDVIQLRRRVGMIFQK
                     WNPFPKSIYENVVYGLRIVGIHDRHYLDEVVENSLRRAALWEEVKDNLNKSAAEMSGG
                     QQQRLCIARALAVEPEVLLMDEPCSALDPISTAKIEELIHELKDTFTIVIVTHNMQQA
                     ARVSDFTAFFYLGELIEFGATGKLFTAPEKRQTEDYITGRFG"
     gene            4674..5360
                     /gene="phoU"
                     /locus_tag="VMZ02_08130"
     CDS             4674..5360
                     /gene="phoU"
                     /locus_tag="VMZ02_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HUR71940.1"
                     /translation="MTTKHTDKKYEEELSRLREEILYMGGLVEDQIQKAIKSLVERDS
                     DLASIIIDRDHEVNRLDVDIDELCIKLLALHQPAGIDLRFITTGLKITTDLERIGDMA
                     VNICERALELNQEPQLKPYIDIPRMARISQRMIHESLDAFVREDTDLALKVCKDDDDV
                     DQLNAQIFRETLTFMLENAQTISRATKISSVSKYLERIADHATNIAEMVIFMVKGKSI
                     RHLKQLPPSV"
     gene            5367..6086
                     /locus_tag="VMZ02_08135"
     CDS             5367..6086
                     /locus_tag="VMZ02_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HUR71941.1"
                     /translation="MGNSVYKILVVEDEADIRKLVHYNLTQDHYKVVEAENGDQALKI
                     IQRDKPHLVVLDLMMPGMSGLEVCRTLRGQNETAKLPILMLTAKAAEADRVLGLELGA
                     DDYLAKPFSPRELVARVRAILRRTNGAAQTETLPAYERGGLKIDFATYEVSVRGKIIK
                     LTLKEFELLKFLVQNPNRVLNRDQLLDRVWGGETFVTPRTVDVHIRRLRKAVEKDDSR
                     PKWILTLRGVGYKFDEKALES"
     gene            6064..>7467
                     /locus_tag="VMZ02_08140"
     CDS             6064..>7467
                     /locus_tag="VMZ02_08140"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HUR71942.1"
                     /translation="MKRLWNLSLVNKVFLSYFAVALLLVIGFYFSSNSLIRRFYITTL
                     SGRMEQEAHLLGRVLPFGVEGNELDALCRQLAGELGSRITVIALDGRVLGDSAESSAK
                     MENHRGRPEIVDAIKSGSGSAQRYSTTVGFDMLYRAFYQRGEKQERIVRIAIPLKDLD
                     GVIRGMRRSLFMGLALASVAGLLLARWFSNYLSSRVQRLVEFSGKLAQGTFPRDFFPG
                     HQRDEMGLLERHLNDMSHRIYNNLMEITGEKEKADSILRCMIEGVVVLDPKGNVLVIN
                     DRAKEMFHVPAGREIHGASVLELSRHPDIRAILDEVVKFDFTSNTYRTEVELDDGAWF
                     VVNAAPLRTPLRMTLGSILVFHDVTEIKRLETVRTDFVANVSHELRTPLTAIQGYVET
                     LIHTPPVDPEVQRQFLEIIERHAERLGRLTEDLLTLSDLEAGKIQITPRPIDTLALIS
                     QVLEVFWERAAKRGVSLS"
BASE COUNT         1801 a         2072 c         1942 g         1652 t
ORIGIN      
        1 ttaaatcgct caaagagatt cagactctgc tcggcgattg gcatgaccgc gcggtgctgc
       61 aaaagcatgt cgaaaaattt atcagacgtc ctggtttttt gaccgaccgg cccgatagtg
      121 gacgaatctt actggcagag ttggaaaagg aacgtttgcg caacgatagc gaggtgacgc
      181 gcgttttaca aaacgccggc aaagtgcagc acagctggac aatttggaaa gccagtgggg
      241 cacatgagtg agcactcggt cgctgcctaa tcgccgttgc gggcgataac acgatcgacc
      301 tgtcggcgca aagtttcaaa cacttcttct atagaattcg aagcatcgac aatctcaaag
      361 ccattcggtt cgaccatctt gtcaaactcc gccaacaacc atttctgata acgaatgaaa
      421 ctttcgtaaa ggtcgtcgcc catgcgcaga tccatgcccg actcccaata gtcgaagccg
      481 ccactctgca ggacccgggt cactaagtcg tcgatagcga cgcgcaagta gaaaatcgca
      541 tcggatgcga agccatagac ctcgcgcagc cactccgggt ccataacgcg cacgattgcc
      601 cgcgccagtg tcaagcactg catggccatg acagcgcagc ttaaccgagt cttaacgcca
      661 tcgttacgcc agcgatacaa ttcagcttta ttttccgcgt cgaagcaata cgatctaaat
      721 gatgccgccg tcgtagttgc caaactcaag tgacttaacg gcaccttaaa taacctgtaa
      781 ccgtttcgta acattggctc gctaaacatc agaaaagagg agaaatcgac atgaaaaata
      841 tattcctggc tttatgccta ttaactctgg ccgggataag tccccgagca gctacggcgc
      901 aagtaatcca gatcgacggt tccagcactg tgttcccggt taccgaagcg gttgccgagg
      961 agtttcagaa gtctaaaaaa ggcaaagtca aagtgaccgt cggcatcgcc ggtaccggtg
     1021 gtgggtttaa aaagttctgc cgcgcagaaa ccgacattag caacgcctcc cggccgattc
     1081 tcaaatccga gatcgaggac tgtaaaaaaa gcggagtgca gttcatcgag ctgccagtcg
     1141 cgtacgacgc tcttaccgtc atcgtcaacc cgaaaaacga ttgggtcaaa tcactggcag
     1201 tcgctgatct caaaaaaatg tgggagccga gcgcacaggg caaagtaacc acctggaatc
     1261 agatccgacc tgaatggccc aacaatccat tgaagctgtt tggtcctggt gccgattcgg
     1321 gaacttttga ttacttcacc gaagcgatcg ttggcaaggc taaatcgagc cgcggtgatt
     1381 tcactgccag cgaggatgac aacgtgttgg ttcaaggcgt tgccaacgat cgtaacgctc
     1441 ttggttactt cggcttcgct tactacatcg aaaaccaatc taagctgaag gcggtcgcta
     1501 tcgaaaccgg caaaggagca gttgtaccct cggcaaagac cgtcgaagac ggtacctatc
     1561 agccgctatc gcgcccgatc tttatctacg tcagcaagaa atccctggat acaaagccag
     1621 aagtgaaaga gttcgttgac ttctatctga agaacgccgc gaagctagtt aaagacgtaa
     1681 agtatgtcgc cttgccccag agtgcgtata ccgtcggcgc cgagcatgtg agcaagggca
     1741 aaatcggcac ggtattcggt ggcaccgcag aagtcggcgt caaaatcgac gacctgctaa
     1801 agcgcgaagc aaacttttga acgaccctcg aaccggaggg gtgcgtggcc ctttcccaat
     1861 aaccttttga gaactgaaac caaccctcat ggcaaacact gaagtagccg tcgagttatc
     1921 cgccgtgcgc caccacattc gcgctcattc acggagcatg acgtcccaga agattcgcga
     1981 gcggatcatc gaatcggcca tgtttctgtg cgctgttttt tccgttgcga taaccgcggg
     2041 tattgtctgg gtgctagtct acgaatcttg gaattttttc cgcaacgtat cgatgattga
     2101 tttcctgacg gatacacagt ggacggtgct attcgaaaac ccgcgctacg gcatcatgcc
     2161 gctggtgacc ggaactctgg ttacctcgtt cattgctctg atcgtggcgc tgcccctcgg
     2221 gacgacgatt gccatttact tgagtgaata cgccaatccg acggttcggg agatcctcaa
     2281 accgattctc gaacttttga gcgcggtccc aacggtggtc tttggttact ttgccctgct
     2341 gtttgttacg cccatgctgc agagaatctt gcccgatctt cctggcttca acatgctgag
     2401 cgcaggcatc gttatcggca tcatgatcgt tccttatgtc agttcgctca gtgaagacgc
     2461 catgaaggcg gtgccagtca ctatccgtga gggttcctac gcaatgggcg ctactcgggt
     2521 gcaaaccgcc ctccgtgtct tgatgcccgc cgcattctca ggaattgctt cggcgtacgt
     2581 attgggcttt tcgcgtgcgg tcggcgagac gatggtggtg gcgattgccg cgggaactca
     2641 acccaattta accctcaatc cgatggaagg cgcggcaact attaccgctt atatcgtcca
     2701 agtgagccta ggcgatctgc cgcatggcag tgttgcatat cagagtatct ttgccgccgg
     2761 tttaaccctg atggtgatga cgttgatatt taacatcggc ggctacttcc tgcgcaagcg
     2821 cttcagggag gtttactaat tatggaaaca ccatcagtag cggcaatcga aaaacgcgtc
     2881 gccgccaata aagcccgcga tcaaaccttc gcgatcatcg gcctgatttg caccttggtc
     2941 ggcatgctca cgctagcggc tttgctcttc gatctcgcga gcgacggtct cgcgcgcatc
     3001 aagccgcact ttttcacctc tttcccatca cgcttcccag gccaagccgg gattctttct
     3061 tcgtgggtcg gcaccattct cgtcatgatc gtcacggcaa tggccgcggt tccgatcgga
     3121 atcggcgccg gtgtttatct cgaggactac gcgcccaagc actggcttac ggcgctaatt
     3181 gaaatcaaca ttgccaacct cgcgggcgtg ccgtcgattg tttacggctt gatggccctt
     3241 ggactgttcg tttaccagtt taaatttggc caaagcatca tgtcagcagg gcttacctta
     3301 gccttgctaa ttttgcctat cgttatcgtt gccacccggg aagccatccg cgctgttccg
     3361 ggaagtatgc gggaagcggc ttacgccctc ggcgctacga aatggcagac taccagcgac
     3421 cacgtcctgc cctattccat gggcggcata ttgaccggcg tcatcattgc cttatcgcgt
     3481 gccatcgggg aaacggcgcc gctcatcacc atcggagcct tgaccttcat cgccttcttg
     3541 cccgatgctc cagtaaaagc agagtttcct tacatctcct tcgactggct atggtcgccc
     3601 tattcggtga tgccgattca aatgttcaac tgggtgtcac ggccgcaggc cgagtttcat
     3661 ctcaacgccg cggcaacagg gctaattttg atgatcatga ccctggctat gaatggcgtc
     3721 gccatctatt ttcgctatcg cttccgcaaa cgcatcaagt ggtagagggc tggaaacgaa
     3781 atgacaaaca gggctaatga agttaagatg agtctacaag aaccgaacca agcagttacc
     3841 gcggaacgcc caacgatgaa agctctggac agctctatga tcagagccag ttctgaaact
     3901 cagcataaag atattttcag cgttgagaac gtcgacttct actacggcca aaaacgcgcg
     3961 ttaaactcga tcaatttggc gatcgccgaa aaaaaggcaa ctgcgttcat tggtccgtcc
     4021 ggctgcggca agaccaccct actgcgctgc tttaatcgca tgaacgatct agtgttaggg
     4081 acccgggtca ccggacaaat catgctcgac ggcaaagata tctacgagcc gacgaccgac
     4141 gtaatccaat tgcgccgccg cgtcggcatg atttttcaaa agtggaatcc cttccccaaa
     4201 agtatttatg agaacgtcgt ctatggcctg cgcatcgtcg gcattcacga tcgccactat
     4261 ctggacgaag tagtcgagaa cagtctgcgc cgcgccgcgc tttgggagga agtcaaagac
     4321 aacctgaaca agagcgccgc cgagatgtcc ggcggtcagc agcagcggct ttgtatcgcg
     4381 cgcgcactgg cggtggaacc ggaagtatta ttgatggacg agccttgttc cgcgctcgat
     4441 ccgatctcaa cggcaaaaat cgaagagctg atccacgaac tcaaagacac cttcaccatc
     4501 gtcatcgtca cccacaacat gcagcaggcg gcgcgagtgt ccgacttcac ggcatttttt
     4561 tacctgggcg aactcataga gtttggcgcg accggcaagc tgttcacggc gccggaaaag
     4621 cgccaaaccg aagattatat caccggccgc ttcggctgat gctgggagaa accatgacga
     4681 cgaaacacac agacaagaaa tacgaagaag agctatccag actgcgtgag gagatccttt
     4741 acatgggcgg gctggtcgag gatcaaatcc agaaggcgat caaatcgctg gtcgagcgcg
     4801 attccgatct cgccagcatc atcatcgacc gcgaccatga agtgaatcgg ctggatgtcg
     4861 acatcgacga attgtgcata aaacttctag ccctgcacca gccagccggc attgacctgc
     4921 gctttattac caccggcctc aaaattacga ccgatctcga gcgcatcggc gacatggcgg
     4981 tgaacatttg cgagcgggcg ttggaactca accaggagcc gcagcttaaa ccgtacatcg
     5041 atattccgcg tatggcgcgc atctcgcagc ggatgattca tgaaagcctg gacgccttcg
     5101 tccgcgaaga taccgatctc gcgctgaaag tttgcaagga cgacgacgat gtcgatcaac
     5161 taaatgcgca gatttttcgc gagaccctga cattcatgct ggaaaacgct cagaccatca
     5221 gccgcgcaac caagatcagt tcggtctcga aatatctcga acgcatcgcc gatcacgcga
     5281 ccaacatcgc tgaaatggtc attttcatgg taaaaggaaa aagcatccgc caccttaagc
     5341 aactgccgcc gtcagtttaa tgaaaaatgg gcaatagcgt atacaagatc ctggtcgtcg
     5401 aggatgaggc cgacattcgc aagctggtgc attacaatct gacccaggac cactacaaag
     5461 tcgtcgaggc ggaaaatggc gaccaagcgt tgaaaattat tcagcgcgat aagccacact
     5521 tggtcgtcct cgacttgatg atgccgggca tgtcgggatt ggaagtctgc cgcacgctgc
     5581 gcgggcaaaa cgagaccgcg aaactaccga ttctcatgtt gaccgccaag gccgctgaag
     5641 ctgatcgcgt cctcggtctt gaattaggcg cggatgacta tttggctaaa ccattcagtc
     5701 cgcgggaact ggtcgcacgc gtcagagcga ttttgcgccg gaccaatggc gcagcacaaa
     5761 cagagacatt gccggcatat gaaaggggcg ggttaaaaat cgattttgcc acttacgaag
     5821 tatcggtgcg cggcaaaata atcaaactga ccctgaagga gttcgaacta ttgaaatttc
     5881 tcgttcagaa tcccaatcgg gtactcaacc gcgaccagct actcgatcgt gtctggggcg
     5941 gcgaaacctt cgtcaccccg cgcaccgtcg atgtccatat tcgccgcttg cgcaaggctg
     6001 ttgaaaaaga tgatagtcga ccgaaatgga tacttaccct gcgcggagtc ggttacaagt
     6061 tcgatgaaaa ggctctggaa tcttagcctc gtcaataaag tttttctctc ctactttgcg
     6121 gtcgccctgc tgcttgttat cggtttctat ttttcctcca atagtctcat tcgcaggttt
     6181 tacataacga cgctcagcgg ccgaatggaa caagaggccc atctgctcgg tcgagtattg
     6241 cccttcggtg tagaaggcaa cgaactcgac gcgctctgcc gtcaactcgc cggtgaactg
     6301 gggtcgcgca tcaccgtcat cgctctcgat ggccgagtct tgggagactc cgcggaaagt
     6361 tcggctaaaa tggaaaacca tcgtggccgc cccgagatcg tcgacgccat caaatccggc
     6421 agcggaagcg cgcaacgcta cagcaccacg gtcggcttcg acatgcttta ccgggcgttt
     6481 tatcagcgcg gcgagaagca agagcggatc gtccgcatcg ccatacccct caaagacctc
     6541 gacggcgtga tccgcggcat gcgccgctcg ctgttcatgg gattggcgct tgcttcagtg
     6601 gccgggctat tgctcgcccg gtggttttca aactatctga gctcgcgcgt gcaacgactg
     6661 gtcgaatttt ccggcaagct cgcccaggga accttcccgc gagatttttt tcccggccat
     6721 cagcgtgacg aaatgggcct cctcgaacgc cacctgaatg acatgagcca ccggatctat
     6781 aacaatctaa tggagattac cggcgaaaaa gaaaaggccg attcgatttt gcgctgcatg
     6841 atcgagggtg tcgtcgtcct cgatcccaag ggcaatgtgc ttgtcataaa cgaccgagcg
     6901 aaagaaatgt ttcatgtgcc ggcgggccgg gagatccatg gcgcctcggt tttagagctg
     6961 tcgcgccatc cggatatccg ggcaatcttg gatgaggtcg tgaaattcga cttcaccagc
     7021 aacacctacc ggacggaggt ggagctggac gatggggctt ggttcgttgt caatgcggcg
     7081 ccgctgcgca ctcctctgcg catgactctg ggctcgatat tggtgtttca cgatgtcacc
     7141 gagatcaaga ggcttgaaac ggttcgcaca gactttgtcg ccaatgtctc gcacgaactg
     7201 cgcacgccgc tgacagccat ccagggctat gtagaaaccc tgattcatac gcctccggtt
     7261 gatcctgaag tacaacgcca atttctcgag atcatcgagc gccatgccga aaggctgggc
     7321 cggttaaccg aagacctact gacactctcc gatctcgaag cgggcaagat ccaaatcaca
     7381 ccgcgcccca tcgacaccct agcgcttatt tcgcaagtct tggaagtttt ttgggagcgc
     7441 gccgctaaac gcggagtttc tctcagc
//
LOCUS       DATLCH010000277         3108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4424895, whole genome shotgun sequence.
ACCESSION   DATLCH010000277 DATLCH010000000
VERSION     DATLCH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4424895"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..376)
                     /locus_tag="VMZ02_08145"
     CDS             complement(131..376)
                     /locus_tag="VMZ02_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71943.1"
                     /translation="MRIFFITLMLFLSPGFGFTEDVQLARVDSRCVDAACVQPANHSK
                     RVLADFSPTPVQLLLSGIALIAVRLLAKKYLPRKVAE"
     gene            776..1687
                     /locus_tag="VMZ02_08150"
     CDS             776..1687
                     /locus_tag="VMZ02_08150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HUR71944.1"
                     /translation="MTKVQSLRDAISSHVRDGMSIALGCGLESLIPFAASHEIIRHHR
                     KNLTLIGPISDMQFDQLIGAGCVRKIQASWVGNVAAGLGHNFRRAAELGEPVPLEIEE
                     HSNFTIGLALQAAAMGLPFLPTRSIKGSDISREAHFETVQCPFTGEQLLAVRAIRPDL
                     AILHVQRADDQGNAQIWGNFGVMREAAMAAEKVILTCEELVDHALILSDPNRNVIPGF
                     VVTSVVHQPNGSFPSPVQGFARRDDAFYFEYHQATRTVPGTDTWLTKYVYTIRDHEEF
                     LEALGEDRREALRPEGDLFAPAVSFNY"
     gene            1716..2150
                     /locus_tag="VMZ02_08155"
     CDS             1716..2150
                     /locus_tag="VMZ02_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HUR71945.1"
                     /translation="MKTVPTAKISQEGCDIIVNAAIEKAKELKQIGSICVVDAGAHLM
                     AFKRMTNGRTHNTLIAQAKARCAALTTAATGKTGRDGSEIPDHQVLAQTLAVGPGYFI
                     SIEGGLPITVDGQCIGGVGFSGAPAAVDRQIVQAGIDAFNKA"
     gene            2217..2972
                     /locus_tag="VMZ02_08160"
     CDS             2217..2972
                     /locus_tag="VMZ02_08160"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HUR71946.1"
                     /translation="MNYTLRELMVVAAAREIHDGDKVFVGMRLPLLGFAVAKETHAPN
                     AIGIFENGVIRDWPALESIFTMSDPPNIARALCCGGLTDVMYLLQSGRVDLGFIGGAE
                     IDRYGNLNTHWVEENGKRTRLPGSGGAADIAVMAKRSIIIMNHERRRFVDKVHYITSP
                     GFGTGGDWRSRQGISGGGPSRAITSLGVFCFDPETHEMILASSHPGIAIVDIKKETGW
                     PLRVAADILETAPPSAAELSAVRKYDPKRVWTA"
BASE COUNT          740 a          840 c          861 g          667 t
ORIGIN      
        1 gggtaaacga tgtagttgat tttgcgccct tgcgtgaggc gcaggccgtt gtgaagctac
       61 gctaagcccg agctgtctac ggatccaccg aaacgaatac ggcaggattt aaacaagcga
      121 atggtgcttc tcactcggcg actttgcgcg gcaggtactt cttagccaaa agccgaacgg
      181 cgatcagagc gataccggaa agcagcagct gcacaggcgt ggggctgaaa tcggccagca
      241 cccttttgct gtgattggcg ggttgaacgc atgcggcgtc gacgcaacgc gagtcgacac
      301 gggcgagttg cacgtcctca gtgaagccaa aaccaggtga tagaaacagc attaacgtta
      361 taaagaaaat tctcatggtg tgatgcttaa aacacttctt tcaagttgcg gaattgttga
      421 gaattactag caactcagat gcccattgca agcaacgaga gacaattttt tagaataaag
      481 cgctcgtcgt gaattccagt gacaaaaacg acaccatcgg tgcctaattg ctcgtccgat
      541 ggacagcctg aagtttttca tctaccaatt cgtcacgctc gggtccgggc tcaagcgcct
      601 catgagtaaa tgctgcgaga taaaacgggc gaattgtaca tgcagcgttt tgagatagct
      661 ttgactgggt tataaaaagt ccctgatgat tcgcactttc aaaacatacg gaccgaataa
      721 ccagcagaga ttttgaaatg ttgatcaacg acttttgaca gggtatccct aggcaatgac
      781 gaaagtgcaa tccttacgcg atgcaatttc cagccatgtt cgtgatggca tgtcgatcgc
      841 gctcggctgt ggactggagt cgctcattcc gtttgccgcg agccacgaaa ttatccgtca
      901 ccatcgcaaa aatttgacgt taatcggacc gatctccgat atgcaattcg atcagctcat
      961 cggtgctggg tgcgttcgca aaattcaagc gtcttgggtg ggcaatgtcg ctgcggggct
     1021 tggtcacaac tttcgccgcg ccgctgagct tggcgagccg gtaccgctgg aaatcgagga
     1081 gcattctaac tttaccatcg gtctagcgct tcaggcagcc gcgatgggcc tgccattttt
     1141 gccgacacga tcgatcaagg gcagcgacat ctccagagaa gcgcacttcg agaccgtcca
     1201 gtgtccattt accggcgaac aattgctcgc ggtgcgcgcc attcgccctg acctcgccat
     1261 attgcacgtt caacgagcag acgatcaagg caatgcacaa atctggggca atttcggtgt
     1321 catgcgcgag gcggccatgg ccgccgaaaa ggttatcctg acctgtgaag aactcgtcga
     1381 tcacgcgctg atactcagcg atccaaatcg taatgttatc ccgggtttcg tcgtcactag
     1441 cgtcgtgcac cagcccaacg gttcctttcc ttcaccggtt caaggatttg cccgacgcga
     1501 tgacgcattt tatttcgagt accaccaggc gacacgcaca gtcccaggga ccgacacctg
     1561 gttaactaaa tatgtttata cgatccggga tcacgaagag tttctcgagg cgttgggcga
     1621 agatcgtcgg gaagctttgc ggccggaggg cgatctattt gcgcccgcgg ttagcttcaa
     1681 ttactaatct taggagcaat ttttaggagg caatcatgaa aaccgtaccg acagcaaaaa
     1741 tttctcagga gggttgcgat atcatcgtca acgcagccat cgagaaggcg aaggaactca
     1801 aacaaatcgg tagcatctgc gtagtcgatg ccggcgcgca cctgatggcg ttcaaacgta
     1861 tgaccaatgg ccgaacgcac aatactctga tcgcccaggc caaagcgcgc tgcgccgcct
     1921 tgacgacagc cgcaacaggc aaaaccggcc gcgacggcag cgaaatcccc gatcaccagg
     1981 tgttggcgca aaccctcgcg gtcggcccag gctatttcat aagcatcgaa ggcggcttgc
     2041 cgattactgt cgacggacag tgcattggcg gtgtcggctt tagcggcgca ccggcagccg
     2101 tcgaccggca aatcgtccaa gccggcattg acgcgttcaa taaagcttag actaattttg
     2161 cgtcgcggat gaccctttac tcgaaatcta aacgtgcagc gcaaatctgc tgacaaatga
     2221 actacacact gcgcgaactc atggttgtcg ccgccgcccg ggaaatccac gatggcgaca
     2281 aagtttttgt cggtatgcgt ttgccgctgc tgggtttcgc ggtcgccaag gaaacccacg
     2341 cccccaacgc catcggtatt tttgagaacg gcgtgattcg cgactggccc gcgctggagt
     2401 cgatctttac gatgagcgat ccgcccaata tcgcccgcgc gctctgctgc ggcggtttaa
     2461 ccgatgtcat gtatttgctg cagagcgggc gagtggatct gggatttatc ggcggtgccg
     2521 aaatcgatcg ttacggcaac ttgaacacgc actgggtgga agagaacggc aaacggacgc
     2581 gcctgccggg cagcggcggt gccgccgaca ttgcagtgat ggccaagcgt agcattatta
     2641 ttatgaacca cgagcggcgc cgcttcgtcg acaaagtcca ttacatcacc tcgccaggct
     2701 ttggtaccgg cggcgattgg cgatcgcgtc aaggaatttc tgggggcggt ccgagccggg
     2761 ctattaccag cctgggagtg ttttgcttcg acccagagac gcacgagatg atcctggctt
     2821 cgagtcatcc aggcattgcc atcgtcgata tcaagaagga gaccggttgg ccgctccggg
     2881 tcgcggccga tattctagaa acagcaccac cgagcgcggc tgaacttagc gcggtacgga
     2941 aatacgaccc aaaacgtgtt tggaccgcgt aaccgtccgg ggcgaaaacc taggcagcgg
     3001 tccttccgcc gccaccgcaa tcgcggagag cagcggaaaa caccaaccac atagctattg
     3061 ttcgagcttg gcagaattga tggtgaccgg ctgcatcggt acgtcctg
//
LOCUS       DATLCH010000278         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2960519, whole genome shotgun sequence.
ACCESSION   DATLCH010000278 DATLCH010000000
VERSION     DATLCH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2960519"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VMZ02_08165"
     CDS             <1..517
                     /locus_tag="VMZ02_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71947.1"
                     /translation="DKVAAIACSAGAEVIRENAENGETSAVDFARLELKRAGCEAVLI
                     LPADMPLLCAQDVEAVLAKVPVDALPPYALLVPSHDQLGTNALLLAPPDVIQLRFGHD
                     SFNFHLSQVAAQGLPVRFVENEHIALDVDEPKDLERFLAYGYQGGESTGIARELLSGC
                     VVPIGQGSGDA"
     gene            514..1266
                     /gene="cofE"
                     /locus_tag="VMZ02_08170"
     CDS             514..1266
                     /gene="cofE"
                     /locus_tag="VMZ02_08170"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HUR71948.1"
                     /translation="MKRIELFGLAGMAEVHAGDIVGQLICEACERQQFELTDGDLLVV
                     AQKIVSKAEGQITALATVTPTARAQQLARELGKEPELVEVILRESRKVIRTGGRAMIV
                     ETHHGFVCANAGVDQSNVGLNQVALLPQDGDRSARQIRAEIRRRIGKNVGIIISDSFG
                     RAWRVGTVDVAVGVAGINPIKDERGLKDRHGYELKAAVAAVADELTSAAELVMGKRDA
                     VPVVIVRGYDIQFTEEGSVHKLLRPEAEDLFR"
     gene            1279..1941
                     /gene="npdG"
                     /locus_tag="VMZ02_08175"
     CDS             1279..1941
                     /gene="npdG"
                     /locus_tag="VMZ02_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HUR71949.1"
                     /translation="MDNFSVAIVGGTGNLGGALALRLGAPGVRIIIGSRDAEKAKKAV
                     ETLQPKLRAGEMIGTTNQEAVKDANFVVIAVPYEGQAQMVQDLKGQVAGKIIIDTVVP
                     LNKVKPFVPAAGSALQEAQQILGDEAPVIGALHNISAVDLGDVESPLGDVLVCGDNAE
                     AKQKVMEIIQRIGAIAYDAGPASNAYVIEGLTGVIIALNRKYKSKHGSIKITGLGAQS
                     GH"
     gene            2253..2828
                     /locus_tag="VMZ02_08180"
     CDS             2253..2828
                     /locus_tag="VMZ02_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HUR71950.1"
                     /translation="MESSKDLGDEELVTLCQQGQGHYFDVLVRRYMEKAFHIAYDFTR
                     NKETAKDLSQDAFLRAYANIKRFDGRSSFYTWFYRIVVNLCLDHARRSGRVFWESLDK
                     APEEPGGQRQLVDTQHQPDQEAIAGEASRRANATLESMPNKQRTAFLLRNQEGLPIAD
                     IAKVMKTTEGTVRVYLHRAVATLRQSLVEFV"
     gene            2837..3397
                     /locus_tag="VMZ02_08185"
     CDS             2837..3397
                     /locus_tag="VMZ02_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HUR71951.1"
                     /translation="MENRIKPLEDACKNYEEDLVLYHYAETDASERAAIEQHLAQCLR
                     CRNFVEDLGRVLPQMSLKPELPQAFWDNYFRETVAKLDDQAARQNWWQRWLAPMNSWL
                     VPTFGTVAIAVFAIALMIQKGDFNATSETASGQIPAEVLVDSNQLEFFKSMEILESLS
                     TLEEQDSAKPERKTSYIENGPVQVVA"
     gene            3419..3682
                     /locus_tag="VMZ02_08190"
     CDS             3419..3682
                     /locus_tag="VMZ02_08190"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HUR71952.1"
                     /translation="MKSRNLFLVVIGFVLLLESRALADGDDWRKLNPKEKDKIRRNYE
                     RWEKLPPQDKEHLREEWERWNRMPQDRRDRLKQRFEDHRRNGD"
BASE COUNT          970 a         1063 c         1237 g          910 t
ORIGIN      
        1 cgataaggtg gcggcgattg catgttcagc cggtgcagaa gtcattcgcg agaacgccga
       61 gaacggcgaa accagtgcgg ttgattttgc gcggcttgaa ctaaagcggg ctggctgcga
      121 agcggtgctg attcttcctg ccgacatgcc gctgctgtgc gcgcaagacg tcgaagcggt
      181 cttggcgaaa gtcccagttg acgctcttcc gccgtacgca cttctagtcc cgtcgcatga
      241 ccagctcggc accaacgcgc ttttgttggc acccccggat gtgatccagc tacgctttgg
      301 ccacgatagt ttcaattttc atctgagcca agtggctgcc caaggtttgc ctgtccggtt
      361 tgtcgagaat gaacatatcg cgctcgatgt cgatgagccc aaggacctcg aacggttcct
      421 ggcgtacggt tatcaaggcg gcgagtcgac cggcattgcc cgggagctgc tgtctggctg
      481 cgtggtgccg atcggccagg gctctgggga cgcatgaagc gaatcgagct tttcggcttg
      541 gccggcatgg cggaagttca tgccggggac atcgtcgggc aactcatttg cgaagcctgc
      601 gagcgccagc agttcgagtt gaccgacggc gatctgctgg tggtggcgca gaagattgtt
      661 tccaaagccg aaggccaaat aaccgcgctg gcaactgtca cgccgacggc gcgggcacag
      721 cagctggcgc gtgaactcgg caaggaacca gaactcgtag aagtcatcct tcgcgaaagc
      781 cgcaaggtaa ttcgcaccgg cggccgggcc atgatcgtcg aaactcatca tggctttgtc
      841 tgcgccaacg cgggagtcga tcagtccaat gtcggcctga accaagtggc gttgttgccg
      901 caggatggcg atcgttcggc gcgtcagatt cgcgcggaga ttcgccgtcg tattgggaag
      961 aatgtcggta ttatcattag cgatagcttt ggccgcgcct ggcgtgtcgg cacggtcgat
     1021 gtggcggtcg gggtggccgg aatcaatccg atcaaagacg agcggggtct gaaagaccgc
     1081 cacgggtatg agctcaaagc agcggtggcg gcggtcgccg acgagttgac atccgccgcg
     1141 gagttggtaa tgggtaagcg cgacgccgtg cccgtcgtca tcgtgcgcgg ctacgacata
     1201 caatttacgg aggaagggtc ggtgcataag ctcttgcgcc ctgaggcgga agatcttttt
     1261 cgctagattg gaaaatatat ggacaacttc agtgtagcta tcgtcggtgg cacaggaaac
     1321 ctcggaggtg ccctggcgct gcgtttgggc gcgccgggcg tgagaatcat tattgggtcc
     1381 cgcgatgccg agaaagccaa gaaggccgtg gaaacgttgc agcctaaatt gcgcgccggc
     1441 gagatgatcg gcacgactaa tcaagaggcg gtcaaagacg ccaatttcgt cgtcatcgcg
     1501 gttccctacg agggccaggc gcagatggtt caggatctta aagggcaagt ggcgggcaag
     1561 attatcatcg ataccgtggt gccgctcaac aaagtcaaac cgtttgtccc tgccgccggc
     1621 tcggcgttgc aggaggcaca gcagatcctc ggtgacgaag caccggtaat tggcgcgttg
     1681 cataatattt cagccgtcga tctcggcgat gtcgaatctc ccctcggcga cgttctcgtt
     1741 tgcggcgaca atgccgaggc aaagcagaaa gtgatggaga tcatccagcg catcggcgcc
     1801 attgcctacg atgccgggcc ggcgagcaac gcttacgtca tcgagggctt gaccggtgtc
     1861 atcatcgccc tgaatcgcaa gtacaaatca aagcacggca gtatcaagat caccggcctc
     1921 ggcgcacaat ccggacattg agcgccacct gttgcaaaac tgggtggagc cgttgccggt
     1981 tgatttagat cggttgaacg ggatgtggtc ctgctcgacc ggcgcgcgca tcgggattct
     2041 cgatcttata tgaaatgtgc ttaatataat cgcttctcag taatgcattt cccaaagttc
     2101 cgggcgctcg cagtcgagcc ccaggcggac tggaactctc aacaattcat gggcttagtc
     2161 agtttcctcg agcgaccgag cagtgttaac ctttggctcg ctgcggcgtc taattagtta
     2221 aacggctggt gcagtgacca gcttctgcag ccatggaaag ctctaaggat ctcggcgacg
     2281 aagaattggt gaccttgtgc cagcaagggc aaggccatta tttcgatgta ttggtgcgac
     2341 gttatatgga aaaggcattt cacatcgctt acgacttcac gcgcaacaag gagacagcca
     2401 aagatctctc ccaagacgcg ttcctgcgcg cctacgcgaa catcaagcgt ttcgatggac
     2461 ggtcgagttt ttatacgtgg ttttatcgca ttgtggtgaa tctttgtctg gaccatgcgc
     2521 ggcgcagcgg ccgggtgttt tgggagtcac tggataaagc cccggaagaa ccgggcggac
     2581 agcgtcagtt ggttgacacc cagcaccaac ccgaccagga agccatcgcg ggcgaagcga
     2641 gcagacgcgc caatgcgacg ttggagtcga tgccgaataa acagcgcacg gcatttctgc
     2701 tgcgaaacca ggaaggattg ccgattgcgg atattgccaa ggtgatgaaa accaccgagg
     2761 gaactgtgcg ggtttatctt catagagctg tggcgacatt gcgccaaagc ctggtggagt
     2821 tcgtttagga gaagttatgg aaaaccggat taagccgctg gaagacgcgt gcaaaaatta
     2881 cgaagaggat ctggtgctgt atcactacgc tgagaccgac gctagtgaac gcgcggccat
     2941 agagcaacat cttgcccagt gtttgcgttg ccgcaatttc gtcgaggatc tcgggcgggt
     3001 actgccacag atgtcgctca aaccggagct gccccaggca ttttgggaca actattttcg
     3061 cgaaacggta gcaaaactgg atgatcaagc agcgcggcag aactggtggc agcgctggtt
     3121 ggcacccatg aatagttggc tcgtgccgac cttcggcacg gttgcgattg ccgtctttgc
     3181 gattgcgctg atgattcaga agggcgattt taacgccacg agtgaaaccg cgtcagggca
     3241 aattcccgca gaagtgttgg tcgattcgaa ccagctcgaa tttttcaaat ccatggaaat
     3301 tttagaatct ctcagcacgc tcgaagaaca ggatagcgct aagccggagc ggaaaaccag
     3361 ttatattgaa aacgggcctg tgcaggtggt tgcctaaata attttgatca ggactaaaat
     3421 gaaatcgcgt aatttgtttt tggtagtcat cggtttcgtt cttttgcttg aatcgcgcgc
     3481 gctcgccgac ggcgacgact ggcgtaagct caatcccaaa gaaaaggata agatccgtcg
     3541 caattatgag cgctgggaaa aattgccgcc gcaagataaa gaacatctgc gcgaagaatg
     3601 ggagcggtgg aatcgcatgc cgcaggaccg tcgggatcga ctcaagcaac gtttcgaaga
     3661 tcatcgccgc aacggggatt gatccacgaa tattccgtct ttcatttccg caagataacg
     3721 atccctctcg cgtaatctag tcttcaatag aaattcgctt ctcgcctttg cgccgaagtc
     3781 gattcggcgc gcgtaaaatt tttcctgccg gtctctagta cttcttatgc tggttaattt
     3841 tttctaagga cggtgcgaaa tcccgccgtc gccgtctaaa gatttgagga aacaaaaaga
     3901 tgacgacaaa agtatgaccg cattcgccgc aactagagac tacggcatgg aaaggaggca
     3961 gcatgaaaac aatcgcgctt ttggtcttgt tggcgttggg aatcgtttcg gcgtccggtt
     4021 gccactggat gcatcaccgc cgcgattatt cgagaagtta cgatcgaaat taaaccctga
     4081 cgtggctccg gtttcgttaa gaagacggag ccaagcccaa taatcacgca aaaaacaaat
     4141 ggtaaaacgc tggtgcaaat tcccagagaa ttataatagt
//
LOCUS       DATLCH010000279        10883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1471507, whole genome shotgun sequence.
ACCESSION   DATLCH010000279 DATLCH010000000
VERSION     DATLCH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471507"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..676)
                     /locus_tag="VMZ02_08195"
     CDS             complement(239..676)
                     /locus_tag="VMZ02_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71953.1"
                     /translation="MEERVTSLFQPDTLLPDQYLDTFRRKLHLEPEKKLMLAILEDAV
                     ACFQKYLFARDSKGKTQFRDTEDWIFQGADLGIFSFDIVCETLGLEPNYVRRGLAHWK
                     KQQQLTRQTQAQVISLRPSRGKLPNRQAVSGRHKPKLRRAAGH"
     gene            complement(765..1220)
                     /locus_tag="VMZ02_08200"
     CDS             complement(765..1220)
                     /locus_tag="VMZ02_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HUR71954.1"
                     /translation="MGLMQIPRRVDYGLRAVIYLSGQDPEKCCSIAEISREQGVPRKF
                     LEKIIQDLIRGGLIKSKRGSCGGYALARAPEAISFSDVIEAIEGPIAVNACMDEHLGC
                     DQISRCTMIGVWSEVQRKVNEVLSSTTIAGLRARPCRELIGSSSLSSVA"
     gene            complement(1234..2442)
                     /gene="nifS"
                     /locus_tag="VMZ02_08205"
     CDS             complement(1234..2442)
                     /gene="nifS"
                     /locus_tag="VMZ02_08205"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HUR71955.1"
                     /translation="MDVYFDNNATTRVLPEVAEAMAPFYTEFYGNPSSIHRFGSGVGQ
                     RIDQARAQVAALLGAADPVEVIFTSSGTEGDNAAIRGMLEARPHKRHIVTTRVEHSAV
                     VGLCQHLEKKGYRVTWLSVDPEGGLDLAELSAALSDDTALVSVMWANNETGVIFPIDQ
                     IGAITRAKGVPLHVDAVQAAGRISIDVSALGIDLLTISGHKFHAPKGVGALYVRRGIS
                     FPPFLIGGHQERNRRAGTENVAGIIGMGMAAEIALNRISEDGAHVMKLRDRLESLLLT
                     SCPESRVNGRKQPRLPNTLNISFRYLEGESMLVLLDQQGICASTGSACTAGSAEPSHV
                     LRAMDVPADWLQGAVRFSLSRFNTEAEVAYVNEKVPAIVQRLQGLSALGRLGSQRCPP
                     DEQRSAGARR"
     gene            complement(2472..3926)
                     /gene="sufB"
                     /locus_tag="VMZ02_08210"
     CDS             complement(2472..3926)
                     /gene="sufB"
                     /locus_tag="VMZ02_08210"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HUR71956.1"
                     /translation="MGSATVEDLARREYKYGFVSDIEADTIPAGLNEDVIRLISARKN
                     EPEFMLEWRLKAYRHWSKLETAEAEPKWAKVHYPAIDYQAIRYYSAPKSQNDGPKSLE
                     EVDPDLLETYAKLGIPLKEQERLAGVAIDAVFDSVSVATTFKSKLAEMGVIFCSFSEA
                     VHQHPELIKKYLGSVVPYTDNFFAALNSAVFSDGSFCYIPKGVKCPMELSTYFRINAA
                     DTGQFERTLIVADEGATVSYLEGCTAPMRDKNQLHAAVVELIAHKDAQIKYSTVQNWY
                     PGDKEGKGGIYNFVTKRGKCLGDRSKISWTQVETGSAITWKYPSCILQGDDSVGEFYS
                     VALTNNYQQADTGTKMIHIGKNTKSTIISKGISAGHGQNTYRGLVKIMKGASGARNYS
                     QCDSLLLGDKCAAHTFPYLEVLNNAAQVEHEASTSKIGEDQLFYCRQRGISNEDAVNM
                     IVSGFCKQVFKELPMEFAVEAQKLLGVSLEGSVG"
     gene            complement(3944..4273)
                     /locus_tag="VMZ02_08215"
     CDS             complement(3944..4273)
                     /locus_tag="VMZ02_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HUR71957.1"
                     /translation="MATGVSVTERAAARIKELAASQNREGQGLRVKVVGGGCSGLQYK
                     VDFDVPKGTDKVFEKDGAKVLVDMKSLLYLTGTELDYKDELMQSGFVFQNPNVKKACG
                     CGASFTV"
     gene            complement(4317..5531)
                     /locus_tag="VMZ02_08220"
     CDS             complement(4317..5531)
                     /locus_tag="VMZ02_08220"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF010611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HUR71958.1"
                     /translation="MALQTPIYLDNHATTPVDPRVLEVMLPYFTERFGNAASKSHAFG
                     WEADAAVDRAREQVAKLIGAVSPREIVFTSGATESDNLAIKGVAEAYREKGNHVITCV
                     AEHKAVLDSCKVLAKHGFELTYLPVLADGLIDMERVRDAITDKTILISIMAANNEIGT
                     IQPVKEIGRLAKAKKILFHSDATQGVGKFPINVEEMGIDLLSLTAHKMYGPKGVGALY
                     VRSSNPRVKLASVIDGGGHERGMRSGTLNVSGIVGLGTACELSQKEMSAEAERLIALR
                     ERLKDGLLEQLDDVQINGHVAQRLPGNLNMSFAFIEGESLLMGLKDIAVSTGSACTSA
                     SLEPSHVLKAIGLADELAYSAIRFGLGRFNTAEEIDYTVMRVVEEVRRLREMSPIYKA
                     KKSKPQPANAKR"
     gene            complement(5619..8168)
                     /gene="uvrA"
                     /locus_tag="VMZ02_08225"
     CDS             complement(5619..8168)
                     /gene="uvrA"
                     /locus_tag="VMZ02_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HUR71959.1"
                     /translation="MAGQIVIQGARVHNLKNIDLEIPRDRLVVVTGVSGSGKSSLAFD
                     TLYAEGQRRYLESLSADTRQLLKQLAKPDVDRIDGLSPAIAIQQKTAALNPRSTVGTL
                     TDIYDFLRLLFARVGQPFCVNCGREIHAQSIEQITDRVMALPRGSRILILAPVRLTAG
                     EAGKSLEEMMRQGFTRVMLDGQNYELAGEIPVAVQQATELDLVIDRLVLRDGVEKRLA
                     DSLEVAARVGQQTVKIKVLGDDNVVPTEQLVFTQKFVCVGCGTTLGDVTPALFSFNRP
                     EGACVACKGTGIEIKRDKRVSNETDVRPCSVCRGTRLGKESLAVRLGGENIAAFTAAS
                     IIDNLTFLDRLELAPERRAVGEKIIAEIASRLRCLAELGLDYLSLDRPTTTLSGGELQ
                     RVRLATQIGSVLAGVLYILDEPSIGLHQKDNDRLIGLLRRLRDTGNSVVVVEHDPETI
                     RAADYVVDMGPGAGVNGGEVVAYGTPAELQKNHRSLTGRYLARELEIALPARRRAGSG
                     SFLKITGVRQRNLKNLTVQFPIGAMTCVTGVSGAGKSTLVMEVLCQTVSQRLQKRGGK
                     TLDSQHVSGWENFDRVIAIDQSPIGRTARSNPATFMGVHDHLRELFAQIPEARVRGFK
                     AERFSFNVRGGRCETCQGEGVTRVEMQFLPEMYVPCESCKGRRYNRETLAIKYKGLSI
                     ADILDLTVNQAAELLSAVPAIHQRLRMLREVSLSYLTLGQSALTLSGGEAQRLKLARE
                     LARRSNGRSLYLLDEPTTGLHFDDIRQLLELLNRLIDLGNTMIIVEHNLEVIKCADFI
                     IDLGPGGGMAGGEIVARGTPEQIVAVTASATGRYLQAVLVPEANSSIDSRT"
     gene            complement(8177..9532)
                     /locus_tag="VMZ02_08230"
     CDS             complement(8177..9532)
                     /locus_tag="VMZ02_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HUR71960.1"
                     /translation="MNPMVFREYDIRGLAEIDFDDQFALLLGRVHGTDIVGKGGTRVA
                     VGRDCRATSDAYAEAVIAGFVSVGLHVYDLGVCPSPLLYFSLFHLDLDGGIQITASHN
                     PSAYNGFKICRGKDTLYGEQIQQLRRTMERGEFRPRAGGKVERYEIVVPYHKHLLADV
                     TKLARPLKVVVDAGSGVGGPVAPPIFRHLGCQVWEVACVPDGNFPFHHPDPTVPENLT
                     LLIDKVRKEKADVGIAYDGDADRIGAVDEQGNILWGDELLVIFARDMLRRNPGALVIS
                     EVKCSQRLFDDIAEQGGRSIMWKAGHSLLKAKMKETGALLAGEMSGHIFFKERYFGYD
                     DAVYASLRLVEILAASGKPLSALLADLPKTVSTPELRVVCADDKKFIIAEKATQYFRQ
                     HYDVVDVDGVRVQFPDGWGLIRASNTQPALVLRFEAQSAAKLEEYRTLIENKLKEFEL
                     Q"
     gene            complement(9529..10551)
                     /gene="pdxA"
                     /locus_tag="VMZ02_08235"
     CDS             complement(9529..10551)
                     /gene="pdxA"
                     /locus_tag="VMZ02_08235"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HUR71961.1"
                     /translation="MPAKPIIAITMGDPSGIGPEIILKALMSPLIKKVSRPLILGDWG
                     VLQRTRVTGRRPKLICWQAGQRLLPMLDDPNAFTVCSLSALSPRDSRPGVGAKAAGHG
                     AFSYIRVAAKLALSGVADAIATAPISKNILIDAGYNYPGHTELLGELGGTPECRMMLI
                     GDKLRVVPVTGHIPFAKVPRSLNRKNIQKTFELTYASLNLSFGIARPRIAVAALNPHG
                     GEKGIFGSEEIEIIAPAVRAARKRGIPVHGPFPADSLFYQAARGDYDAVVCMYHDQGL
                     IPLKLHHFYGGVALTLGPPFIRTSVDHGTAYDIAGKARADETSMTEAILLASRLARQN
                     SRLKII"
     gene            complement(10624..>10883)
                     /locus_tag="VMZ02_08240"
     CDS             complement(10624..>10883)
                     /locus_tag="VMZ02_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71962.1"
                     /translation="PAQRSVLSYLNPLSYIVKETPFEDEDQQKGPLANKSVVSIFGTP
                     LFVTDNPAEAPDIFAPPAEPQSKGIFSSIVDSLNPFSSKKP"
BASE COUNT         2418 a         3147 c         2889 g         2429 t
ORIGIN      
        1 taggtaaatc gctcgacggc cgtgaacggc acatgtggca agcatgtgat aaatccgcgc
       61 aacgaatatt cgccgatacg gtaacgcagg tcgtaaacaa attgtgccgc cggcgagcgc
      121 cgacgctttc tcttagccat gaacacttat gaattcgtcg cgcacgcttt gcaagttgat
      181 tctatgcgaa atcggcggct ctggttaatc gcgccggtgc atagggagaa ataaacgctt
      241 agtggcccgc agcccggcgc aatttgggtt tgtgtcggcc ggacacggcc tgtcgattgg
      301 gcagttttcc gcggcttggc ctcagagaaa ttacttgagc ttgtgtctgg cgcgtgagct
      361 gctgctgttt tttccagtgc gccaggccac gccgtacata gttcggctcg agacctaacg
      421 tctcgcagac gatatcgaag gaaaaaatac ctaagtcggc gccctgaaaa atccaatcct
      481 cggtatcgcg aaactgcgtc tttcccttgc tatcgcgggc gaacaaatat ttctgaaaac
      541 aggcgacagc gtcttccaat atcgcgagca tcaacttttt ctccggttcc aagtgcagct
      601 tgcgccgaaa tgtatcgaga tattgatcag gcagcagagt atccggctga aatagcgaag
      661 ttacgcgttc ttccatattc aatcctgtcc cgtaagccat gaaacccacc tcccattaga
      721 gtgcggcaga gaccgaggtc gcattgcgag aacatgcatc ctggttatgc aacactcgaa
      781 agagaagagg agccaataag ttcccggcaa ggccgggctc ttaacccggc gatcgttgtg
      841 ctgctgagca cttcattaac ttttctttgc acttcgctcc acactccaat catcgtgcat
      901 cgagatattt gatcacagcc caagtgttcg tccatacatg cattgacagc gatgggacct
      961 tcaatagctt caatgacatc gctaaaggaa atcgcttccg gcgcacgcgc caacgcatag
     1021 ccgccgcacg aaccgcgctt cgacttgatg agaccgccgc gaatcaaatc ttgaatgatc
     1081 ttttccaaaa atttccgtgg cacaccttgt tctctggaga tctcggcgat ggagcaacac
     1141 ttctccgggt cttgacccga caagtaaatc acggcacgca gtccataatc gacccgccgc
     1201 ggaatctgca taagccccat aatttaactc cctctatcga cgtgcgccgg cactgcgctg
     1261 ctcgtccgga ggacagcgtt ggctccccaa tctgcccaga gccgaaaggc cctgtaagcg
     1321 ttggacgatt gccggcacct tttcgttaac gtaggcgacc tctgcctcgg tattgaatcg
     1381 actcaaactg aagcgcaccg caccctgcag ccagtcggcc ggcacgtcca tcgcccgcaa
     1441 aacatgcgac ggctcggctg atcccgccgt gcaggccgag ccggtggaag cgcaaatgcc
     1501 ctgctggtcc aacagaacga gcatcgactc gccttctaga tagcgaaaac tgatgttcaa
     1561 cgtgttcggc aaacgcggtt gctttcgacc gttgactcgg ctctccggac acgaggtcag
     1621 cagaaggctt tccaagcggt cgcgcagctt catcacgtgc gcgccatctt cggagatgcg
     1681 attcaatgcg atctcagcag ccattcccat gccgataata ccggcgacgt tttccgttcc
     1741 ggcgcggcga ttgcgctctt gatgcccgcc gatcagaaat ggcgggaatg aaatgccgcg
     1801 ccgcacgtac aatgcaccca ctcccttggg tgcatgaaat ttatggccgg agatggtcag
     1861 taaatcgatg cctagggcgc ttacgtcgat ggagatcctg ccagcagctt ggaccgcatc
     1921 gacgtgtaag ggaacccctt tagcacgcgt gatcgcgcca atctggtcga tcgggaaaat
     1981 cacaccagtc tcgttgttcg cccacatgac cgagaccagg gcagtatcat cggacagcgc
     2041 cgcagataac tcggctaggt caaggccccc ttctgggtcg acgctcaacc aggtcacccg
     2101 gtaacctttt ttctcgagat gttggcacaa gccgaccacc gcggagtgtt cgactctagt
     2161 cgtaacaata tggcgcttat gcggccgcgc ttcgagcatg ccacgaatcg ctgcattatc
     2221 accctccgtg ccgctgctgg tgaaaatcac ctcgactgga tcggcggcgc cgagcagcgc
     2281 ggccacttgg gcgcgcgcct gatcgattct ctgaccgacg ccactgccga agcgatgaat
     2341 actcgacggg ttaccataaa attcggtgta gaacggcgcc atcgcctcgg cgacttcggg
     2401 caacactcga gtcgtggcat tattatcgaa ataaacgtcc attgaattca gacaaccttc
     2461 cggtgaaccg attagccgac actgccctca agactgacgc ctaataattt ctgcgcttca
     2521 acggcaaact ccattggcaa ctccttaaac acctgcttgc agaaaccact gacaatcata
     2581 ttaaccgcgt cctcgttgga gatgccgcgc tgccggcaat agaaaagttg atcttcgcct
     2641 atcttcgatg tcgatgcctc atgctcgacc tgcgcggcgt tgttaagcac ttcgaggtac
     2701 gggaatgtgt gggcagcgca tttatccccc aacaacaacg aatcgcactg tgaatagtta
     2761 cgcgctccgc tggccccttt cataatcttc accaagccgc gataagtgtt ttgaccgtgg
     2821 ccggcggaaa ttcccttgga gatgatcgtg ctcttggtgt tcttaccgat gtggatcatc
     2881 ttggtgccgg tgtcggcttg ttgataatta ttggtcagcg ccaccgagta aaactcgccg
     2941 accgaatcat cgccttgcaa aatgcagctc ggatacttcc aggtaatcgc cgaaccggtt
     3001 tccacctgcg tccaggaaat cttcgagcga tcccccaagc acttgccgcg cttggtgacg
     3061 aagttgtaga taccgccctt gccttcctta tcgcccggat accaattttg taccgtggaa
     3121 tatttgatct gggcgtcttt gtgcgcgatc aactcgacga ccgccgcgtg cagctgattc
     3181 ttgtcacgca tcggcgcagt gcagccctca agatagctca ccgtcgcacc ttcatcggcg
     3241 acgatcaacg tgcgttcaaa ttgtccggtg tcggccgcat tgatgcgaaa ataggtcgac
     3301 aactccatcg ggcacttgac gcccttcggt atgtagcaga acgagccgtc gctgaagacc
     3361 gccgagttga gcgccgcgaa aaaattatcg gtatacggca ccactgagcc tagatacttt
     3421 ttgatcagct cgggatgctg atgcaccgcc tcggaaaacg agcagaatat aacgcccatc
     3481 tcggcgagct tgcttttaaa cgtcgtcgcc acggaaacac tgtcgaatac tgcgtcgatg
     3541 gcgacacccg ctaggcgctc ttgttctttg agcggaatgc caagcttggc ataggtttcg
     3601 agcaggtcgg ggtcgacttc ctccaagctt tttggcccat cgttctgtga cttgggcgcg
     3661 gaatagtagc ggatcgcttg ataatcgatc gccggataat gcaccttcgc ccatttgggt
     3721 tcagcctcag ctgtttccag cttactccaa tgacgataag ccttcagacg ccactcgagc
     3781 ataaattccg gctcgttttt ccgtgccgaa atcagtcgaa tgacatcttc atttaacccc
     3841 gccggtatgg tatcggcctc gatgtccgaa acgaaaccat acttatattc gcgccgggca
     3901 agatcttcga cagtagcaga tcccatgttt gctccttccg ttttcacacc gtgaacgacg
     3961 cgccgcagcc gcaggccttc ttgacattgg gattctggaa aacaaatccg gattgcatta
     4021 gttcgtcctt gtaatcgagt tcagtgccgg tcaaatacag taagcttttc atgtcgacca
     4081 aaaccttggc gccgtctttc tcgaaaacct tatcggttcc cttgggcaca tcgaagtcaa
     4141 ctttgtattg caatccggaa cagccgccgc cgacaacttt tacccgaagg ccctggcctt
     4201 cccggttctg gctggccgcc aactctttga ttctcgccgc tgcccgttcg gtgacactca
     4261 cgcccgtcgc catgatattt ctcctgtcaa aaacgccaat ggcttaataa aattcgctat
     4321 cgctttgcgt ttgccggctg cggtttgctt ttctttgctt tgtagatcgg tgacatttcg
     4381 cgcagtcgcc gcacctcttc gacgacgcgc ataacggtgt agtcgatttc ctctgcggtg
     4441 ttaaatcggc cgagtccgaa acgtatcgcg ctgtaagcta actcatcggc aagcccgatc
     4501 gccttcaaaa cgtgcgacgg ctctaaactc gccgaagtac aggcggagcc ggtcgacacc
     4561 gcgatatcct ttagacccat taaaagcgat tcgccctcga taaaagcaaa gctcatattc
     4621 aagtttccgg gcagacgctg agcgacgtga ccgttgatct ggacgtcgtc aagctgctcg
     4681 agaaggccgt ccttcaggcg ctcgcgcaaa gcgatcaaac gctctgcctc ggcgctcatt
     4741 tccttttgcg ataactcgca ggctgtgccc agtccaacga tgccggagac gttcaacgtt
     4801 cccgaacgca tgccgcgctc atgaccgcca ccatcgatga ccgatgccag ctttacccgg
     4861 gggttgctcg atcgtacata aagcgcgccg acgcctttag ggccgtacat cttatgcgcc
     4921 gtaagcgaca atagatcgat tcccatctcc tcgacattga tgggaaactt gccaacaccc
     4981 tgggtcgcgt cgctatgaaa taaaattttc ttcgctttgg ccaaccggcc gatctcttta
     5041 accggttgga tcgtgccaat ctcgttgttt gccgccataa tcgagatcag gattgtcttg
     5101 tcggtgatgg catcgcgcac gcgttccatg tcgatcaaac cgtccgcgag aaccggcaga
     5161 taggtcagct cgaaaccatg ctttgccagt accttacaac tatccaatac cgccttgtgt
     5221 tcggcgacac aggtgatgac gtgattgcct ttttcgcgat aggcctcggc aacgcctttg
     5281 attgccaaat tatccgactc ggtggcgccg ctggtaaaga cgatctcacg gggcgaaacg
     5341 gcaccgatga gtttggcgac ttgctctcgg gcgcgatcca ccgcggcatc ggcctcccaa
     5401 ccgaaggcat ggcttttact cgctgcatta ccaaagcgct cggtaaaata cggtaacatt
     5461 acctccaaaa cgcgtgggtc taccggcgtg gtggcgtgat tatcgaggta tattggagtt
     5521 tgcagagcca ttgacggtca acttcattcc agaccggatt ggtctgatat caaagataac
     5581 caagagtgcg aaacgagtca atacctagcg tacgataact aagtccgcga atcgatggac
     5641 gaattcgcct caggtacgag aacggcttgc aggtagcggc cggtcgcaga cgccgtgact
     5701 gcgacgattt gctctggtgt gccgcgtgca acgatctctc cgccggccat tccgccgccc
     5761 gggccgagat cgattatgaa atcggcgcac ttaatcactt cgagattatg ttcgacaata
     5821 atcatcgtgt tgccgagatc gattagacgg ttgagcagtt cgaggagttg tcggatgtca
     5881 tcaaaatgta gacccgtggt cggctcatcg aggagataga gtgatcttcc attggaccga
     5941 cgcgccaact cgcgcgccag cttcagccgt tgcgcttcgc cgccggaaag cgtcagggca
     6001 gattggccca gggttaagta acttaggcta acctcgcgca acatacgcaa gcgttggtga
     6061 atcgccggca cggcacttaa cagttcggct gcttggttca ccgtaagatc caaaatatcg
     6121 gcgatgctca ggcctttata cttgatagcc agcgtctcac ggttataacg ccgtccttta
     6181 catgattcac aagggacata catctctggc aaaaattgca tctctacgcg ggtgaccccc
     6241 tcgccctggc aggtttcgca gcggccgccc cggacgttaa aggaaaatcg ttcggctttg
     6301 aagccgcgta cccgcgcctc cggaatttgc gcgaacagct cacggaggtg gtcgtgcact
     6361 cccataaatg tcgcggggtt cgatcgcgct gtacggccaa tcggcgattg atcgatagcg
     6421 atgactcggt cgaaattttc ccaacctgag acatgctggg aatcgagcgt cttaccgccc
     6481 cgtttttgca accgctggct gaccgtttga caaagcacct ccataactaa agtactcttg
     6541 ccggcgcccg atactccggt tacacaggtc atcgcgccaa tggggaactg caccgtgaga
     6601 ttttttaaat tgcgctgccg aacgccggta atttttaaga aactcccact gccggcgcgc
     6661 cggcgcgccg gcagcgcgat ttccagctcc cgcgcgagat atctgccggt cagcgagcgg
     6721 tgatttttct gcaattcggc gggagtgcca taggcgacga cttcaccgcc attgacgccc
     6781 gctccgggtc ccatgtcgac gacgtaatcg gctgcgcgga tggtctcggg gtcatgctcg
     6841 acaacgacca cggagttgcc ggtgtcgcgc agtcggcgca gcaagccgat caatcggtca
     6901 ttgtctttct ggtgcaaacc aatgctcggc tcgtcgagaa tatacaggac accagcgaga
     6961 accgaaccga tctgagtcgc caaccgcacg cgctgcagct ctccgccgga aagcgttgtt
     7021 gtcggccgat ccaaactcaa ataatcaagt cccagttccg ctaaacagcg taaccgactg
     7081 gcgatctcgg caatgatttt ttcgccaacc gctcggcgtt cgggagcgag ttcgagtcga
     7141 tcgagaaacg tcagattgtc gatgatcgac gccgcggtaa atgccgcaat attttcgcca
     7201 ccgagacgca cggccaagct ttctttgccc aagcgcgtac cgcgacaaac cgagcacggc
     7261 ctgacatccg tctcattgct gacgcgcttg tcacgtttga tctcaatgcc ggtgcctttg
     7321 caggcgacac aggcgccttc cggacgatta aaagaaaaca aagccggggt gacatcacca
     7381 agcgtcgtac cacagccgac gcagacaaat ttttgagtga aaaccaactg ctcggtcggc
     7441 acaacattat catcgccgag aactttaatt ttcaccgtct gttgcccgac tcgcgcggct
     7501 acttccaacg aatcggccaa gcgcttctca actccgtcgc gcagcaccag gcgatcgatt
     7561 accagatcga gttcagttgc ctgctgcacg gccacaggaa tctcgccggc aagttcatag
     7621 ttttgtccgt cgagcatcac tcgggtaaat ccttgacgca tcatctcttc taaactcttc
     7681 cccgcctcgc ctgctgtcag tcgcactggc gctaagatta aaattcgcga cccccgcggc
     7741 agcgccatca ctcgatcggt gatttgctca atcgactgcg cgtgaatttc acggccgcaa
     7801 ttgacgcaaa acggctgccc gacgcgggca aacagaagtc ggagaaaatc ataaatgtcg
     7861 gttaacgtgc caacggtgga acgcggattc aaggcggcgg ttttttgttg aattgctatg
     7921 gccggcgaca acccgtcgat gcgatcgacg tcaggcttgg ccaattgctt gagcagctga
     7981 cgcgtatcgg cgctcaagga ttcgagatag cgccgctgcc cttcggcata gagcgtatcg
     8041 aaggcgagtg atgacttccc ggaaccggag acaccggtaa cgacgaccaa ccggtcgcgt
     8101 gggatctcca gatcgatgtt cttgagattg tggacgcgcg cgccctggat gacgatttgt
     8161 cccgccatcg cgacgattac tgcagctcga attctttaag tttgttctcg atcagcgtgc
     8221 gatactcctc gagcttggcg gcggactggg cttcgaatcg aagcaccaat gctggttgag
     8281 tattggacgc tcgaatcaac ccccagccat cgggaaactg cacgcgcacg ccgtcgacat
     8341 cgaccacatc gtaatgctgg cgaaaatact gcgtcgcctt ctcggcaatg atgaatttct
     8401 tatcatcggc gcaaacgaca cgcagttccg gtgtcgagac ggttttgggc agatcggcaa
     8461 gcagcgccga aagcggcttg cccgaagcgg cgaggatctc gacgagacgc aatgacgcgt
     8521 ataccgcatc gtcgtaaccg aaatagcgct ctttgaaaaa gatatggccg ctcatttcgc
     8581 ctgccagtag cgcgccggtc tctttcattt tggctttgag caaagaatgg ccggctttcc
     8641 acatgatcga tctgcctccc tgctcggcaa tatcgtcaaa cagccgttgc gagcatttca
     8701 cttcggagat caccaatgcg ccgggattac gcctgagcat gtcgcgggca aagatcacga
     8761 gcaattcgtc tccccaaagt atgttacctt gctcgtcgac ggcaccgatc cggtcagcgt
     8821 cgccgtcata ggcgatgccg acatcggctt tttcttttct gaccttatcg atcagcagcg
     8881 tcaaattttc cggcaccgtc gggtctggat gatggaacgg gaaattgcca tcgggaacgc
     8941 aagcaacctc ccacacttgg cagcccaagt ggcggaaaat cggcggggct accgggccgc
     9001 cgacgccgct tccggcatcg acaacgacct ttaacggtcg cgcgagcttc gttacgtcgg
     9061 ctagcaaatg cttgtgatag ggcacgacga tctcataacg ctcgacctta ccgcccgcgc
     9121 gcgggcgaaa ttcgccgcgc tccatcgttc gacgaagctg ttgaatctgc tcgccgtaga
     9181 gcgtgtcttt gccgcggcaa atcttaaagc cgttgtacgc cgacggattg tggctggcgg
     9241 taatttgaat accgccgtcg agatcgagat gaaacaagga aaaatacagc agcggcgaag
     9301 ggcaaacgcc gagatcgtaa acatgcaagc caacggagac gaaaccggcg atcactgctt
     9361 cggcgtaggc atccgaggtc gcccggcagt cacgtcccac cgcgacacgg gtcccgccct
     9421 tgccgacgat atccgtgcca tggaccctgc cgagcagtag agcaaattgg tcgtcgaagt
     9481 cgatttcggc tagtccgcga atgtcgtatt cgcgaaatac cattggattc atataatttt
     9541 caatcgagaa ttttgccgcg ctaagcgaga cgccaagaga atcgcttcgg tcatactggt
     9601 ctcatcggct ctggccttac cggcaatgtc gtaggcagta ccatggtcca ccgacgtacg
     9661 gataaatggc ggccccaggg tcagcgccac accgccgtag aaatggtgca gtttgagcgg
     9721 tatcaaacct tgatcgtgat acatgcacac caccgcgtca taatcgccgc gtgcggcttg
     9781 ataaaataaa ctatcggctg ggaacggtcc atggacggga atgccgcgtt tgcgcgccgc
     9841 cctgacagcg ggcgcgatga tttcaatctc ctcgctgccg aagatcccct tttccccgcc
     9901 gtggggattg agtgccgcca cggcaatgcg cggccgggcg atgccgaagg atagattcaa
     9961 gcttgcgtag gtcaactcga aagtcttttg aatatttttg cggttgagac tgcgcggcac
    10021 cttagcgaag ggaatatgac cggtcaccgg gacgacgcgc aacttatctc cgatcagcat
    10081 catacggcac tcgggcgtgc cgccaagctc gccaagcaat tcggtatgac cgggatagtt
    10141 atagccggcg tcgatgagaa tgttcttgct gatgggcgcg gtggcgatgg cgtcagcaac
    10201 tcctgaaagc gcgagtttgg cagcgacgcg aatgtagctg aacgcaccat gcccggcagc
    10261 tttagcgccg acgccgggac gggaatctct gggacttagc gcggataacg aacagaccgt
    10321 gaacgcattg gggtcatcga gcattggcag gagccgttgg cctgcttgcc agcagatcaa
    10381 cttcggcctc cggcccgtca cacgcgttcg ctggagcacg ccccagtcgc cgagaatcag
    10441 ggggcgggaa actttcttaa taagtgggct catcagagcc tttagaatga tttcagggcc
    10501 gatacctgac gggtcgccca tagtgatggc gatgatgggt ttggctggca tgcgatgacg
    10561 acacgacact tcgcgtgaga tagttcgtcg attatctcca cttgcgaatg gtcaggcaag
    10621 agatcagggt ttttttgacg agaacggatt gagcgaatcg acgatcgacg agaatattcc
    10681 cttggactga ggttccgccg gcggcgcgaa gatatccggt gcctcggcag gattatcggt
    10741 gacaaatagc ggcgtcccga agatactgac aacgctcttg ttcgccaatg gccctttctg
    10801 ctgatcctcg tcttcgaagg gcgtctcttt gacgatgtac gacagtgggt tcaaatagct
    10861 tagcaccgag cgttgcgcag gcc
//
LOCUS       DATLCH010000280         5993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2214706, whole genome shotgun sequence.
ACCESSION   DATLCH010000280 DATLCH010000000
VERSION     DATLCH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214706"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="VMZ02_08245"
     CDS             <1..1419
                     /locus_tag="VMZ02_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HUR71963.1"
                     /translation="KGTWKIDVQKKTMITVYKLRPNLKWHDGKPYTSKDFEFGWQIAK
                     HPEFPMPDRLVPEMISKIDTPDDRTLVIHWNDLYNEAYAIQYTHVRAFPRHLLLDAFQ
                     AGDMKAFANLPYWNKNFVGAGAFKVVEWDQGSRVELEAFNDFALGRPKIERITYKTVE
                     DSNTNLAAVMAGEVDLCMRSTISFDGAMILREQWEKPGKGKVLISPASWTWLNLSRDN
                     PWFSDVRVRRALLHGIDRDAMVQNLFKGEKVISHLPLSRARKVNNKALAASTIYKFDT
                     ELAKKLLAEAGWKPGADGVLTNGKGEKMEFEFRVTAERRDHEQAQAIIADYWKKLGVR
                     TNIKNMPNRLLNSADNRNRWPGAYIGSHNVTIEEWPERFHSKNIPTAENKFAPENVSG
                     WNDPKKDAILDDINSIITPEQSEKLQIEFCKMFSAALPHLPLFFTPEVLVVKKGLTGI
                     TPRQESGGQNSSSWNTQTWDKV"
     gene            1521..2492
                     /locus_tag="VMZ02_08250"
     CDS             1521..2492
                     /locus_tag="VMZ02_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR71964.1"
                     /translation="MLLYVLRRVFQSVPLLLIISALIFTLLYLMPGDPLYRMLEGIPN
                     LRPQDYDRLRKLYGFDDPIYVQYFKWLWQLIQLNPGYSREYGQPVFDIILPALKNTLV
                     LTIAAVVLGKLLAITLGIFSAVRQYSIGDYILTATTFVAYSVPAFWLGLMLIILFSVK
                     LGWLPTSGIVNSEVEPGSWAATVDWLKHLILPVAVLAISEIIQVQRFMRSSLLEVLRQ
                     DYLTTARAKGLSETVVIGRHALKNALIPVVTIIAVTMPRVVGGSTVVETVFAYPGMGR
                     LLYTSIMGNDYVVAMTVVMIIAATVVFFNLLADVIYGWLDPRIRYQS"
     gene            2574..3479
                     /locus_tag="VMZ02_08255"
     CDS             2574..3479
                     /locus_tag="VMZ02_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR71965.1"
                     /translation="MTVDSPALIQLNAIKSAEPAGYWRIVWRRFKQHRVAYVGFFFFM
                     SLLLAVIVGPYLVPYEFDQVSLSERMQMPSARHWLGTDELGRDTWARIMHGGRVSLTV
                     GLAVALSTVVIGGLIGILAGYVGGALDNALMRLVDTIYTVPRIVLLLVLSKLAGPGLL
                     NIIIILVALEWTNAARLARSAVLSFREQEFVLAAQCLGASRWRVMLRHLLPNALGPLI
                     VSATLDAGAAIRAETTLSFLGLGIQPPTPSWGNLLTNAATNIFIAPLQVFLPGLFIFF
                     ALMSFNLLGDALRDALDPKMSKSRG"
     gene            complement(3533..4777)
                     /locus_tag="VMZ02_08260"
     CDS             complement(3533..4777)
                     /locus_tag="VMZ02_08260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71966.1"
                     /translation="MVGLVSALRVLGGGLHNYGFTVFFLPISTDLGLNRAETSLAFSL
                     ARAQGAIEGPFVGYLIDRFGPKPMILTAVLLTGVGYVCLGWVHSYTTFLIVYLGIISL
                     SFTPGFVHAPMAVGNTWFIRYRARAMTVISSAVPVGGTLLTPLLAMAVQHWGWRWGAI
                     LGGLLFLLIGIPLGLGVRASPESMGLLPDGAAPDIPLNSISEDRPRTTAVTDDPTLRQ
                     AMKTWIFWLFVVGMTVRVGAYSTISVHFVPIMVWKGVSQEGAALLLASFAFLNWAAHY
                     VIGWYADTSNRPKLLTLCMLAAAVSMLLLILGKGRWPLWAFTVGFTAIDASFPIVWAT
                     IGDFFGRKYFATIRGTMSFFYTWGSVLGPVIAGAAYDRSQSYVATLYGLVAILLLGAV
                     LTALTIKPWQELMDNGKLIKDN"
     gene            4984..>5993
                     /locus_tag="VMZ02_08265"
     CDS             4984..>5993
                     /locus_tag="VMZ02_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71967.1"
                     /translation="MDIRTSSTPTTANIAPPKIAFAALKHKDYRWFLIATMLAMMADN
                     IEHVISYWVLFQKFQSPVLAGFAVISHWTPFLLLSVYFGGLADRYDCRKLIQIAQIMY
                     MGVTAIWALLFLTDTIQIWHACVLLVIHGIAGVFWTPAEILMIHDIVGADDLPSAVRL
                     NATARQLGILLGPAVGGAMMLWLGAPAGLFINAVIYLPLTIWLMLVPYTGHLREGAPA
                     RRGLRWRDAAEVLREVAQHRSIVTMIVLGGAASLFVGNAFQASMPEFAHDLGTDKADF
                     AYSALLAANAAGSVFGGFILDAKQWVPPTVKAAINCAILWCVAMTAFAFSNSYLFSLI
                     LL"
BASE COUNT         1299 a         1617 c         1707 g         1370 t
ORIGIN      
        1 aagggcacct ggaagatcga cgtgcaaaaa aagaccatga tcacggtcta caaactgcgt
       61 cccaatctca agtggcatga cggcaagccg tacacttcaa aagacttcga gtttggctgg
      121 caaatcgcca agcacccgga atttcccatg cccgatcgac tggtaccgga gatgatttca
      181 aagatcgata ctcccgacga tcgaaccctg gtaattcact ggaacgatct ctacaacgaa
      241 gcctatgcga ttcaatatac ccatgtgcgt gctttcccgc gtcatctctt gctcgacgca
      301 tttcaagccg gtgacatgaa ggcgtttgcg aatttgccgt actggaataa gaactttgtc
      361 ggcgctgggg cgttcaaagt cgtcgagtgg gatcagggta gccgcgtcga gttggaagct
      421 tttaacgatt tcgcgcttgg gcggccaaaa atcgaacgca tcacttacaa aacagttgag
      481 gacagcaata ccaatctcgc ggccgtgatg gcgggcgagg tcgatttgtg catgcgcagt
      541 accatttctt tcgacggcgc catgattctg cgcgagcagt gggaaaagcc ggggaagggg
      601 aaggtattga tcagcccggc gtcttggacc tggctcaacc tgagccgcga taatccctgg
      661 ttcagcgacg tcagggtgcg ccgtgctttg cttcacggca tcgaccgcga tgccatggtg
      721 caaaacctct ttaaaggcga gaaggtgatt tcccatctgc cgctatctcg cgcccgcaaa
      781 gtcaacaaca aggccttggc ggcatcgacg atttataagt tcgataccga gttggcaaaa
      841 aaattgttgg ccgaagccgg ttggaagccc ggcgcagatg gcgtcttgac caatggcaaa
      901 ggcgagaaaa tggaattcga gttccgcgtt accgccgaac ggcgagatca tgaacaggcc
      961 caggcgatca tcgccgacta ttggaaaaag ctcggcgtgc gcaccaacat caaaaatatg
     1021 ccgaaccgtc tactcaattc ggcggacaat cgcaaccgtt ggccgggcgc gtatatcggt
     1081 tcgcataacg tcacgatcga agagtggccc gaacggttcc acagcaaaaa catcccgaca
     1141 gcggaaaaca aatttgcgcc ggaaaatgtt tccggttgga acgatcccaa aaaggacgcg
     1201 attcttgacg acatcaattc gatcattacc cctgaacaat cggagaagct gcagatcgag
     1261 ttttgtaaaa tgttcagcgc tgcgctgcct catttgccgc tatttttcac tccggaagtc
     1321 ctcgtggtta aaaaaggtct caccggaatc acgccgcgcc aggagagcgg cgggcaaaat
     1381 tccagctcgt ggaatacgca aacctgggac aaagtctaaa tgacccgttc gtgatcgtgg
     1441 atcgtgttcg tagatgacaa cggcggccga tacgagcacg atcgcgaaca gaacgccttc
     1501 acggcctcag cgatcgatct atgctgctct acgttcttcg ccgggtgttt cagtctgttc
     1561 ctctgttatt gataatctcg gcgctaattt ttactttgct atacctgatg ccgggggatc
     1621 cgctctaccg catgctcgag ggaattccca acttgcggcc gcaggactat gacaggcttc
     1681 gcaagcttta cggttttgac gacccgatct atgtccagta cttcaaatgg ctctggcagt
     1741 tgattcagtt gaaccccggc tactcacggg agtatggaca gccggttttt gatataatcc
     1801 tgccggcgct gaagaacact ctggtgctga ccattgctgc cgtggtgctc ggtaaactac
     1861 tcgccatcac tctcggaatc ttttccgcag tgcgccagta ctcgatcggc gactatattc
     1921 taactgcgac gacttttgtc gcttattcgg ttccggcgtt ttggctcggg ctgatgttga
     1981 tcatcctatt ttccgtcaag ctcggttggt tgccgacttc cggcatcgtc aatagcgagg
     2041 ttgagccggg cagctgggcc gcgacggtgg actggttgaa acatctgatc ttgccggtgg
     2101 cggtgttggc gatctccgag ataatccaag tgcagcgctt tatgaggtcg agcttactgg
     2161 aagtgctgcg ccaggattat ctcaccaccg cgcgcgccaa aggtttaagt gaaactgtcg
     2221 tcatcggccg acatgcgctc aaaaacgcat tgattccagt cgtgacgatt attgccgtca
     2281 cgatgccgcg agtggtcggt ggctcaacgg tggtggaaac ggtttttgct tatccgggta
     2341 tgggacggct gctttacacg tctatcatgg gcaatgacta cgtcgtcgcc atgaccgtgg
     2401 taatgatcat cgccgcgacc gtggtattct tcaatctact ggcggatgtg atttatggat
     2461 ggctcgatcc gcgcattcgc tatcagagtt gatggtgcgc tgcagctttg caagcgcccc
     2521 gccttgtcgt cgctcgcgct gcgcggacga aagaagaggg acgaggggga ccagtgacgg
     2581 tcgatagtcc tgcattaatt cagctgaacg caatcaagag cgccgagccg gcgggctact
     2641 ggcgaattgt ctggcgccgt ttcaaacagc accgagtcgc ctacgtcggc ttctttttct
     2701 ttatgagcct gctgctggcg gtaattgtcg gcccttacct ggtgccctat gaattcgatc
     2761 aagtgtcgct gtcggagcgc atgcagatgc cctcagcacg ccactggctc ggcaccgatg
     2821 agctggggcg cgatacttgg gcgcgtatca tgcacggcgg acgagtttct ttgacggtgg
     2881 gattggcggt ggcgttgagc actgttgtga tcggcggact gattgggatt ctcgccggct
     2941 atgtcggcgg cgcgctcgac aacgctctca tgcgcctggt ggatacgatc tacacggtac
     3001 cgcgcatcgt gttgcttttg gtcttgtcga agctggccgg gcctggtttg ctcaatatca
     3061 tcattattct cgtcgcactg gaatggacta atgcggcgcg cctggcacgc agcgcagttt
     3121 tgtcctttcg cgaacaggag ttcgtcctgg cggcgcagtg tctgggtgct tcgcgttggc
     3181 gtgtcatgtt gcgccacctg ctgcccaacg cgcttgggcc attgatcgtg tcggcgaccc
     3241 tcgatgccgg cgcggcgatc cgcgccgaga cgaccttgag tttcctcggt ctaggcattc
     3301 aacctccgac gccgagttgg ggcaatctgc tcaccaatgc cgcgaccaat attttcatcg
     3361 caccgctgca agtgttcctg cccggactgt ttattttctt tgctctgatg agtttcaatc
     3421 tgctcggaga tgctctgcgt gatgccctcg atccaaaaat gtcgaagagc aggggataaa
     3481 aacaatcgat aatggataat tgagaatggg aaggtttaac ccgaattcgt ctttaattgt
     3541 cctttatcaa ttttccatta tccattagtt cctgccacgg tttaatcgtt agcgcggtga
     3601 gcacggcgcc gagcaggagt atcgctacta aaccgtacaa ggtcgcgaca tagctttggc
     3661 tacgatcgta ggcggcgccg gcgatgacgg gcccgagcac gctgccccag gtgtagaaaa
     3721 acgacatggt gccgcgaatc gttgcgaaat atttgcggcc gaaaaagtcg ccgatggtag
     3781 cccagacgat cggaaacgat gcatcgattg cggtaaaacc gaccgtaaag gcccacaacg
     3841 gccatcttcc ttttcccagg atcagtagca acatcgaaac cgcggcggcg agcatgcaca
     3901 aggtcaaaag cttcggccga ttgctggtgt cggcgtacca gccgatgacg tagtgcgccg
     3961 cccagttcaa gaacgcgaaa ctcgccagta acaacgcagc gccttcctgc gacacacctt
     4021 tccaaaccat gatcggcacg aagtgcacac tgatggtgct gtaggcaccc acgcgcaccg
     4081 tcatgccgac cacgaagagc caaaagatcc aagttttcat cgcttggcgc agtgtcgggt
     4141 catccgtaac tgctgttgtt ctggggcgat cttcggagat gctatttaag ggtatgtccg
     4201 gcgcggcgcc gtcgggtaga agtcccatgc tttcgggcga tgcgcggacg ccgagtccaa
     4261 gcggaatgcc gatcagcaga aacaataagc cgccgagaat cgcgccccag cgccatcccc
     4321 agtgttgcac cgccatggcc aacagcggtg tcaaaagagt gccgccgacg gggactgcgg
     4381 agctgattac ggtcatggcg cgggcgcggt agcgaataaa ccaggtgttg cccaccgcca
     4441 tcggcgcatg gacaaacccg ggcgtaaaag agagcgagat gatgccgaga tagacgatca
     4501 aaaaagtcgt gtagctatgc acccagccga ggcagacata acccacaccg gttaacagca
     4561 ccgcggtgag gatcatcggc ttgggaccga aacgatcgat caagtagccg acgaaaggac
     4621 cttcgatggc gccctgagcg cgcgccaagg agaacgcgag cgaggtttcg gcgcggttca
     4681 aaccgagatc ggtgctgatc ggcaaaaaaa acacggtgaa gccgtagttg tgcagcccac
     4741 cgccgagcac gcgcagcgcc gagaccaaac cgaccatgcg ccagccgatg aaagtttgtg
     4801 ttagtagttg ccggacccgt tccagagtga tgcctcgagg agtgtttcgc tgctgtttcg
     4861 tttctcccag aagcggccga ggatggcaag aaattatttc aagagcgcaa ctcaggcgca
     4921 agtttgcgcc aacgatagat atccgttatt ttgaattaca ccctcgactg atcggacaca
     4981 tcgttggaca tacgcacttc aagcaccccg acaaccgcga atattgcacc gccaaaaatc
     5041 gccttcgcgg cactgaaaca taaggactac cgctggtttc tcatcgctac gatgctcgcc
     5101 atgatggccg acaacatcga gcatgtgatc agttactggg tgttgttcca gaaatttcaa
     5161 tcaccggtgc tcgccggttt cgccgtcatc agccactgga cgccgtttct tttgctgtcg
     5221 gtctatttcg gcggcttggc cgatcgttac gattgtcgca agttgattca gatcgcccaa
     5281 atcatgtaca tgggcgtgac agcgatttgg gcgctattgt ttttgaccga cacgattcaa
     5341 atctggcacg cctgtgttct tttggtgatt catggcatcg ccggagtgtt ctggactccg
     5401 gcggagatat tgatgattca cgacatcgtc ggcgccgacg atttgccgag cgcggtgcgc
     5461 ttgaacgcga cggcgcgcca actgggcatt cttctcggtc cggcggtggg cggagccatg
     5521 atgctctggc tcggcgcgcc ggccggattg tttatcaatg cggtgattta tttaccgctg
     5581 actatttggc tgatgctggt gccgtacacc ggccacctgc gagagggagc gccggcgcgg
     5641 cgcggattgc gttggcgcga cgccgccgaa gtgttgcgcg aagtggcgca gcaccggtcc
     5701 atcgttacca tgattgttct cggcggcgcg gcgtcactgt ttgtcggcaa tgcatttcaa
     5761 gcgagcatgc cggagttcgc ccacgatctt ggcacggaca aagccgattt tgcttacagt
     5821 gcgctgcttg cggccaatgc cgccggttcg gtgtttggtg gttttatcct cgatgccaag
     5881 cagtgggtgc cgccgacggt gaaagcggcg atcaattgtg caattctctg gtgcgtagcg
     5941 atgacagcat tcgcattctc gaacagttac ttattttctt tgatattgtt att
//
LOCUS       DATLCH010000281         5212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9669757, whole genome shotgun sequence.
ACCESSION   DATLCH010000281 DATLCH010000000
VERSION     DATLCH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9669757"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..751)
                     /locus_tag="VMZ02_08270"
     CDS             complement(311..751)
                     /locus_tag="VMZ02_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR71968.1"
                     /translation="MKLNKVYHMGIPVDDLDRAKDFYTQVLGMEYLGRVGGNPNNPDS
                     FPVHGVAQKLDRLRCGDYDIVLFARPRPLNRDAVDQDGIAHQAFSVAWEDYDAALTQA
                     KELGKFHRVVERDSGNTIYMFDSEGNYLELHFARPGGRRQRQAN"
     gene            874..2112
                     /gene="glp"
                     /locus_tag="VMZ02_08275"
     CDS             874..2112
                     /gene="glp"
                     /locus_tag="VMZ02_08275"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HUR71969.1"
                     /translation="MGQAFFKVLAPQEALGLLLAVEPVDKQTIASVQGRGRVLAEDLF
                     SAVDLPHFHRAAMDGYAVRAKDTFGASQSLPAYLKLAGVIDMGKEATQALNKGEAIRI
                     STGGMMPPDADAVIMVEYTDETDGGLVEIHRAVSPGQNVIQIGDDIKKGEAVFSKGRR
                     LRAHDVGALTGVGISSIAVHQRPRVALISTGDEIVDAQVEPRPGQVRNINQHSLAGLI
                     EEVGGELKDWGVIRDDQAQLTEAIGAALDWADAVLLSGGSSMGAKDIALESILSFPDS
                     QFVFHGISIAPGKPTIFAKACGKPILGLPGYPVSALVIFDLFGAPMLRRLGGESRDAL
                     QRFSRTVRAKLTTNISSQIGREDYVRIVMKGEGADLLATPLPSKSGAIFTLVKADGMV
                     RIGMNQDGLERGEEVDVILF"
     gene            2188..4119
                     /locus_tag="VMZ02_08280"
     CDS             2188..4119
                     /locus_tag="VMZ02_08280"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HUR71970.1"
                     /translation="MARKRYLKKTPLNEARELFLSTVNPNSLAIEEIPIDDALDRITA
                     EPVFAKISSPHYHASAMDGICVRAEDTFGATEFIGKKLTLVDGDSPGTDAFKYVDTGN
                     ALPGWANAVIMIEKVHQIDTKTVEIFDSVAPWNHVRLVGEDVVATELLLPRAHRLRPY
                     DMGALLAAGHTTVGVKARPRVGIIPTGDELIQPGEEATPGAVIDFNSTVLAAFIREWG
                     GQPVKYPRAKDDEILLGAALERAAAECDLVTIIAGSSAGEHDLTSDVVAKAGELLAHG
                     IDVMPGKPAVLGALAGKPVLGVPGYPVSAIVIAREILRPALEKFLSNAAPTAPSVRAV
                     VPKKIASHLGLEEFIRVTLGRVGEKLVAVPLARGAGVITTMVHADGLMRIPSLVEGLN
                     AGETAEVELLRPMVDIENTILCTGSHDLAIGVLEDQLKLSHPELKIAASNVGSLGGLL
                     ALQRGETHIAGTHLLDPDSGIYNIPDIQRAIPKVPVILLHLAQREQGILVARGNPKAL
                     RSLEDLARSKIRFVNRQPGSGTRVLLDYELKKLKIDGAAIVGYEREEFTHMAVGVAVA
                     SGLADAGLGVRAAAQALTLDFIPIASEQYDLLIERRFFESPRGEQLLLTICSARFKAA
                     VSALGGYDTSKAGAVLYRQ"
     gene            4375..4572
                     /locus_tag="VMZ02_08285"
     CDS             4375..4572
                     /locus_tag="VMZ02_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR71971.1"
                     /translation="MERTNESLQLLTLIEAAQLLHVSTKTLQRMIRSGDLPALKVGGQ
                     WRLRESQLISWIESRESRSQS"
     gene            4648..>5212
                     /locus_tag="VMZ02_08290"
     CDS             4648..>5212
                     /locus_tag="VMZ02_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HUR71972.1"
                     /translation="MQLPLEKIVQLASGTFLCQLRRRLLVMITIAVVPILVVVLYQAK
                     LRRDVQILEVHETAWRLTHAIAQRQSQLVDSVKQLLVLLTQLPEIGKGNAAECGDFLR
                     RVLAQKQAYFDVGVVDAAGNVHCRARDSGPSIDLVQSSLRRRIFETKDFVIGDFQLLG
                     NWQRRALMFGYPLPNDSGAVQDTLVAAL"
BASE COUNT         1105 a         1384 c         1525 g         1198 t
ORIGIN      
        1 cccgaccagg atcgccgggc cggtgccgcc tagctcggag tcgcgccaac ccatggcaat
       61 gtcgagctgc gattgccgcg gcagctcgat gctgttgaaa aatgtataac cgacgacgtt
      121 agcgatatct acgcgtttcg aagttgcggc gatggagagt ttgatcggtc tgtccatgtt
      181 gtgttcctgc tgactgttct gctgggttga cctcgagttc accaggacgg cacgaaggac
      241 gcgaaggatt tggaacaaat tcccgcactg cgtgctcttc gcgcctttgt ggtgagtgat
      301 tttttcgttt ctaatttgct tgccgctggc gccggccgcc ggggcgtgcg aagtggagtt
      361 cgagataatt gccttcggag tcgaacatgt aaatcgtatt gccgctgtcg cgctcgacaa
      421 cgcggtgaaa tttgccgagc tccttggcct gcgtcaacgc cgcgtcataa tcttcccagg
      481 cgacgctgaa ggcctgatgg gctataccat cctggtcgac ggcatcgcga ttgagcggac
      541 gcggacgggc gaacaggact atatcgtaat cgccgcagcg taaacggtcg agcttctgcg
      601 ccacgccgtg cacgggaaac gaatcaggat tattcgggtt gccgccgacc cggcctaaat
      661 attccatgcc caagacttgg gtataaaaat ccttggcgcg atccaggtcg tccacaggta
      721 tgcccatgtg ataaacctta ttcagtttca tgtgcgaact ccggaagatt ctgattaccg
      781 gcgtgattgt tatcctaagc tggtattgtg tcaagggaat cgccgtcaga tttgcttccg
      841 cgggactctt ctgattatgc tttccagcta gagatgggac aggcattttt caaagtattg
      901 gcaccccaag aagcactggg attgttgctg gcagtcgagc cggtggacaa acaaaccatt
      961 gcatcggtgc agggacgcgg gcgtgtatta gcggaagatc ttttctcggc ggtggatctg
     1021 ccgcactttc atcgcgccgc catggacggc tacgcggtta gagccaaaga taccttcggc
     1081 gcgagtcaga gtttgcccgc ttatctgaaa ctagcgggtg tcatcgacat gggcaaagaa
     1141 gcgacgcagg cgcttaataa aggcgaggcg attcgcatct ccaccggcgg tatgatgcca
     1201 cccgatgcgg atgcggtgat tatggtcgaa tacaccgatg aaacggacgg cgggttggtc
     1261 gaaattcatc gtgctgtgtc gccagggcag aatgtcatcc agatcggtga cgacatcaaa
     1321 aaaggcgaag cggttttttc taaaggacgc cggctgcggg cccatgacgt aggcgccctt
     1381 accggcgtcg gtatttcgtc gattgcagtg caccaacggc cgcgcgtcgc gctgatttcc
     1441 actggcgatg aaattgtcga cgcacaggtc gagccgcggc cggggcaggt gcgcaacatc
     1501 aatcagcatt cactcgccgg cttgatcgag gaggttggtg gcgagctgaa ggattggggc
     1561 gtgatccgcg acgatcaagc tcaattgacc gaagcaatcg gtgctgcact ggattgggcc
     1621 gatgccgtgc tgctctcggg cggcagttcc atgggtgcga aggatattgc cttggaaagt
     1681 atcttatcat ttcccgattc gcagtttgtt tttcacggca tctccatcgc gccaggtaaa
     1741 ccaacgatct tcgccaaggc ttgcggtaag ccaatcctcg gtttgcccgg atacccggtt
     1801 tccgctttgg taatcttcga tttgttcggt gcaccgatgc tgcgccgcct gggcggcgaa
     1861 agtcgcgacg cgttgcagcg ctttagccga actgtgcggg cgaaattgac caccaatatc
     1921 tcgtcgcaaa ttggccggga ggactatgtc cggatcgtca tgaaaggtga gggcgccgat
     1981 ctgttggcga cgcctctgcc cagcaaatcc ggggcaattt ttactctggt taaagccgac
     2041 ggcatggtgc gcatcggcat gaatcaggat ggcttggagc ggggcgaaga ggtggacgtg
     2101 attttatttt agctggagtt tcgtctttct tgttcggcga cgtgaactcg agagctcgta
     2161 agctgcaacg taaaaccgcc tgagaccatg gctcgcaaac gttatctcaa gaaaaccccg
     2221 ctcaacgagg cgcgcgaact gttcttgtcg acagtgaacc caaatagttt ggccatcgag
     2281 gagatcccca tcgacgacgc cctcgaccgg attaccgccg agccggtgtt tgccaagatt
     2341 tcttccccgc attatcacgc ttcggcaatg gatgggatct gcgtgcgggc tgaagatacc
     2401 ttcggtgcca cagagtttat cggcaagaag ctcacccttg tcgatggcga ttctccaggc
     2461 acggacgctt tcaaatatgt cgatactggc aacgcgctgc ccggctgggc taatgccgtc
     2521 attatgatcg agaaggtgca ccagattgat actaaaaccg tcgaaatttt tgactcagtg
     2581 gcaccgtgga atcatgtccg cttggttggc gaagatgtgg tcgcgaccga attgctgctg
     2641 cctcgagcac atcgcctgcg tccctacgat atgggagcgt tattggccgc cggtcatacg
     2701 acagtgggcg tcaaggcgcg gccgcgcgtc ggtatcattc caaccggcga tgaactgata
     2761 cagccagggg aggaggccac gcccggggcg gtgatcgatt tcaactcgac ggtgctagcg
     2821 gcgttcattc gtgaatgggg cggccagccg gttaagtacc cgcgcgccaa ggacgacgaa
     2881 atattgctgg gggccgcgtt ggagcgcgca gcggccgaat gcgatctggt gacgattatc
     2941 gctggctcat cggccggcga acatgacctc acctccgatg tcgttgcgaa ggccggcgaa
     3001 ttactcgccc atggcatcga cgtcatgcca ggcaagcctg ccgtgctcgg cgcgctcgcc
     3061 ggcaagccgg tattgggtgt gccgggctac ccagtatcag ccattgtcat cgcccgcgaa
     3121 attctccggc cggcgttgga gaaattctta agcaatgctg cgccgacggc accctcggtg
     3181 cgcgcggtgg tgccgaaaaa gatcgcctct catctcggtc tggaagaatt tattcgcgtc
     3241 actttgggcc gcgttggcga aaaattggtg gcggtgccgt tggcgcgtgg cgccggcgtg
     3301 atcacgacca tggtccatgc cgacggcttg atgcgcatcc caagtctggt tgaagggctc
     3361 aacgccggcg aaactgcgga agtagagctg ttgcggccga tggtcgacat cgaaaataca
     3421 attctttgca ccggcagcca cgacttggcc attggcgtgc tcgaagatca actcaagctg
     3481 agccatccgg aattgaaaat agccgcgagc aatgttggca gcttgggcgg cctactcgcg
     3541 ttgcaacgcg gcgagactca tatcgccggt acccatctgc tcgatcccga ctccgggatc
     3601 tacaatattc ccgatattca gcgggcgatt ccaaaagttc cggtgatttt gctgcatctc
     3661 gcccaacgtg agcagggtat tttagttgcg cgcggcaatc cgaaagcgct tcgtagccta
     3721 gaagatctcg cgcgcagcaa gattcgcttc gtcaatcgcc aaccgggttc aggcactcgt
     3781 gtgttgttgg attacgagct caaaaaattg aaaatagacg gcgcggctat cgtcggctac
     3841 gagcgcgagg agttcactca tatggcggtg ggcgtggcgg tggcaagcgg actcgccgac
     3901 gccggtctcg gcgtgcgcgc cgcggcgcaa gcattgacac tcgactttat cccgatcgcg
     3961 agcgaacaat acgatttgct catcgaaaga cgtttcttcg aatcaccccg cggcgaacag
     4021 ttactattga cgatatgttc tgcacgcttc aaagcagcgg tgagcgcgct gggcggctat
     4081 gacacctcta aagccggtgc agtgctttac cggcaataaa tccccgtcac tcgtttcaaa
     4141 ttaaattgac cacgccgagt taatccatcg cttggcctct aattttcctc aagccggcga
     4201 caggttgtgc gatgtcatgc gtccggtaat gaacctgata ctatccagtt gttcaatttt
     4261 gtttccaagc acctcaaatc gcaccatctg tcctgtttaa aatgttggaa taacaagggg
     4321 ttagatttcc agtcgctggc attaccattg cgtttgttgg tagcggcgaa aactatggaa
     4381 agaactaacg aatccctcca gctcttgact ctcattgagg ctgctcagtt acttcacgtc
     4441 tcgaccaaga ctttgcaacg aatgattcgc agcggcgatc tgccggcgct taaagttggc
     4501 ggtcaatggc gcctgcgtga aagccaactg atcagttgga tcgaaagccg tgaaagtagg
     4561 tcgcagtcct agcgatccag cggcagatca ttatttccaa aatggaaatt agtgaatagc
     4621 aaaatactca cgaaaaacga aaaattagtg caattgccgc tagaaaaaat tgtccaattg
     4681 gcgagcggaa cgtttctttg ccaattgcgc cggcgcttat tggtgatgat caccatcgcc
     4741 gtggtgccga tccttgtggt cgttctctac caggcgaagt tgcgccgcga tgtgcagatt
     4801 ttggaagtcc atgagaccgc gtggcgcctc acccacgcaa tcgctcagcg gcagtcgcaa
     4861 ttggtcgatt cggtgaagca gttgttggta ttgctgacgc agttgcccga gattggtaaa
     4921 ggcaatgcgg cggaatgcgg cgatttcttg cgtcgggtgt tagcgcaaaa acaagcctat
     4981 ttcgatgtcg gcgtggtgga tgcggccggt aacgtacatt gccgcgcacg ggactccgga
     5041 ccttcaattg acctggtgca aagttcgttg cgccggcgca tctttgaaac taaggacttc
     5101 gtcattggcg attttcaatt acttggtaat tggcagcgcc gggccctgat gttcggatat
     5161 ccgttgccaa atgattccgg cgccgttcag gacaccctcg ttgccgccct gg
//
LOCUS       DATLCH010000282         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_1473892, whole genome shotgun sequence.
ACCESSION   DATLCH010000282 DATLCH010000000
VERSION     DATLCH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473892"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1290)
                     /locus_tag="VMZ02_08295"
     CDS             complement(229..1290)
                     /locus_tag="VMZ02_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71973.1"
                     /translation="MHAAVIFILIFSVLGPHSALWAQSSEAKLASLEKLSPAERQQRL
                     LEAAKNDGEAVIYANMDVTAMKPLADGFMRRYPGVKATSVHFSGAAIITRIESESRVG
                     KPLSDVVLSGQLGVLASIEKRIAARYRSPERETLRDGFKDKDGLWTTYMTNVMVSAYN
                     SRLVKKDELPRSVEDLLKPRWKNKLVMDSQSYVWFGTMMQFLGEDNGLRFMKRLNEQN
                     LQHSRGRRLMTQLLAAGEHEIAVETNLNSVLSLSRQGAPLSFAPLQPYFLSPSLVFMS
                     ANAPHPFAGALFVDYLLSEEGQKIIVTTNRMPAHPKVKSPESQLLEGQDVRMPDIFDI
                     GRRYQQIGTRYREIFPGAR"
     gene            complement(1301..2281)
                     /locus_tag="VMZ02_08300"
     CDS             complement(1301..2281)
                     /locus_tag="VMZ02_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71974.1"
                     /translation="MFRHYRRFVFEALLVFALLYNPMAGHAELFRVALSTKDFGYLPL
                     FVGMRSGLFAQEGLEIQWIVVNSNVVVTALMAGEIDVAGIAGSSMRAAARGAPLKANF
                     FPYYKSLFVLLGAPEIKRVQDLRGKVIGTTGPGATTEVAASMVLQANGMDPKKDVTFM
                     TIGGAETSITAMRSGIIQARAFNPDAALIMKKQGFTELAVLADLGPWPWAGYSTSDAL
                     LANRRDKLKRWTRAMVRSLQLMQTRPDETYKIAQAEFKHPRDITEGAANICIKAIDPK
                     NPGGASDESLRNNIDLTITQPLKLAAQPQLAKLVDFSLLHEVQRELAIRK"
     gene            complement(2283..>3087)
                     /locus_tag="VMZ02_08305"
     CDS             complement(2283..>3087)
                     /locus_tag="VMZ02_08305"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HUR71975.1"
                     /translation="GGTGPAILVGNKKYTFGFGNPVGLSAMAYLGCGFYKKKIPLRAI
                     GVFPTWDRLIFAVRPDTGIESIEDIKKQKYPLRISTRRRGKLATTIYAIEEVLKAYGL
                     SYRDIEKWGGKIMEAPNPSGPERRDAILSGNADAVFDEGVKSWGPTALKAGMRFLSID
                     DNAVKHMAKLGFPSAMLTHKHYPKLDQAIRCVDFSGWTFFCHADLSDKVAYHMARAVD
                     LCHKHIPVDHFDKRPMTIREFCRGGEAGQLNIPLHPGAKKYFREKGYIA"
BASE COUNT          614 a          826 c          925 g          722 t
ORIGIN      
        1 ggaggatacg aagttggggt cgacgacaag ttcgtagcct tcgatgccga ctctgccgtc
       61 ggtgatcgcc tggcagcggg gatggtctgg aagacctagc gtgattttcg tcttggtggc
      121 catgagtttg ctccttcaaa ttagtagttc cagtcgttcc aatggttcca gaggttccaa
      181 tctttttcgg actcctggaa cgactggaac gattgaaact cgccggcact accgggcgcc
      241 ggggaatatc tcccggtagc gggtgccgat ctgctggtag cgtcttccta tgtcgaaaat
      301 atccggcatg cgcacgtcct gaccttccag cagttggctc tccggggatt tgactttcgg
      361 atgagccggc atgcggttgg tggtgacgat gattttctgg ccctcttccg aaaggagata
      421 gtcgacgaac agcgcgccgg cgaagggatg gggcgcgttg gcgctcatga agaccaggct
      481 cggactgaga aaataaggtt ggagcggcgc gaagctcagc ggcgcgccct ggcgactcag
      541 actgagcacc gagttgagat tggtctcgac ggcgatctca tgctcgccgg cggcaagcag
      601 ttgtgtcatc aagcgacggc cgcgcgagtg ctgcaaattt tgttcgttca agcgcttcat
      661 gaagcgcagg ccgttgtctt caccaaggaa ctgcatcatg gtgccgaacc aaacatagga
      721 ttggctgtcc atcaccaatt tgtttttcca gcgcggcttc aagagatctt cgacactgcg
      781 cggcagttcg tcttttttta ccagccggct attgtaagcg ctgaccatga cgttggtcat
      841 gtaggtggtc cacaggccgt ctttgtcttt gaagccatcg cgcaaggtct cccgttccgg
      901 tgagcgatag cgcgcggcga tgcgcttttc gatcgaggcg agcacgccca attgcccgct
      961 caagacgacg tccgacaatg gcttgccgac gcggctttcg gactcgattc tggtgatgat
     1021 cgcggcaccg gaaaagtgca cgctggtggc tttgacgccg ggatatcttc tcatgaagcc
     1081 gtcagccagc ggcttcatcg cggtcacgtc catgttggcg tatatgaccg cctcgccgtc
     1141 gttttttgcc gcttccaaca agcgttgctg acgctcggcc ggcgacagtt tttccagaga
     1201 agcgagtttt gcttcgctgc tctgcgccca gagcgctgag tggggcccga gaacggaaaa
     1261 tatcagaatg aagatcacag ctgcgtgcat gccggcctca ttatttccgg atggctaatt
     1321 cacgttgcac ttcgtgcaat agagaaaagt ccaccagctt agcaagctgg ggttgtgccg
     1381 cgagtttcaa cggctgggtg atggtcaggt cgatattgtt gcgcaacgat tcgtcgctgg
     1441 caccgccggg attcttcggg tcaatggctt tgatgcaaat attcgcggcg ccctcggtga
     1501 tgtcgcgtgg atgtttgaat tccgcctggg ctatcttgta ggtctcgtcc ggccgagttt
     1561 gcatcagttg caggcttctc accatcgccc gggtccagcg tttgagtttg tcgcgccggt
     1621 tggcgagcag cgcatcggag gtcgaatagc cagcccaggg ccagggaccg aggtcggcta
     1681 agactgcgag ttcggtaaat ccctgcttct tcatgatgag cgcggcgtca ggattgaaag
     1741 cgcgggcttg gatgatgcca ctgcgcatgg cggtgatgga agtttctgcg ccgccaatgg
     1801 tcatgaaagt cacgtctttt ttgggatcca tgccgtttgc ctgcaacacc atgctggcgg
     1861 cgacctcagt ggtcgcgccc gggccggtgg tgccgatcac tttgccgcgc aaatcttgaa
     1921 cgcgcttaat ttccggcgcg ccgagaagca caaacaggct tttgtaataa ggaaagaaat
     1981 tggctttgag cggtgcgccg cgcgcggcgg cgcgcatgga cgagccggcg atgccggcga
     2041 cgtcgatctc gccggccatg agcgcagtga cgacgacgtt gctgttgacg acgatccatt
     2101 gaatctccag gccttcctgg gcaaatagac cggagcgcat gccgacgaac agcggtaaat
     2161 aaccgaagtc tttagttgaa agggcaacgc gaaatagttc ggcgtggcct gccattgggt
     2221 tgtagagcaa ggcaaaaact aacagtgctt caaagacgaa tcgtcgatag tggcgaaaca
     2281 ttctatgcga tatatccttt ctcgcgaaaa tatttcttcg ctcccggatg gagcggaatg
     2341 tttagctgtc cggcttcgcc gccacggcaa aactcgcgga tggtcatggg ccgtttatcg
     2401 aagtgatcga ccgggatatg tttgtggcaa agatcgaccg ctctggccat gtggtaggcg
     2461 accttgtccg ataaatcggc atggcagaaa aatgtccaac cgctgaaatc gacgcagcga
     2521 atcgcttgat caagctttgg gtaatgtttg tgcgttagca tggcgctagg aaaccccaac
     2581 ttggccatgt gtttgaccgc gttgtcgtcg atcgacaaga agcgcatgcc cgctttcagt
     2641 gccgttggac cccagctctt tacgccttcg tcaaagaccg cgtcggcatt accggataaa
     2701 attgcatcac gccgttccgg gccgctcgga ttgggcgctt ccatgatctt accgccccac
     2761 ttttcgatgt cccggtagct caagccgtag gccttgagga cttcttcaat ggcgtagatc
     2821 gtcgttgcca gcttgccgcg ccggcgcgtt gagatgcgca acgggtactt ttgttttttg
     2881 atgtcttcga tagattcaat cccggtgtcc gggcggacgg cgaagatcaa gcggtcccaa
     2941 gtcggaaaga cgccgatggc gcgcagggga atttttttct tataaaaacc gcagccgaga
     3001 tacgccatcg ccgacaaacc caccggattg ccgaagccga acgtgtattt cttgttcccg
     3061 accagaatcg ccgggccggt gccgcct
//
LOCUS       DATLCH010000283         4127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_4433023, whole genome shotgun sequence.
ACCESSION   DATLCH010000283 DATLCH010000000
VERSION     DATLCH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4433023"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2077
                     /gene="aceE"
                     /locus_tag="VMZ02_08310"
     CDS             <1..2077
                     /gene="aceE"
                     /locus_tag="VMZ02_08310"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HUR71976.1"
                     /translation="APIMSIYHARFLRYLQSRDLISGEEPRIFCFAGDGEMDEPESSG
                     ALTLASRENLDNLVWIVNCNLQRLDGPVRGNGKIIQELEALFRGAGWNVIKVIWGSDW
                     DDLLAKDDKGLLRKRMEEAVDGDYQKYSVEPGSYTRKHFFGKYPELLEMVNHMTDDQI
                     HKLLRGGHDPMKVYAAYKAAVEHRGAPTVILAKTVKGYGLGEAGEGRNITHQQKKLNE
                     RELREFRTRFDIPLPDEEVVETPFYRPPLDSPETQYLLERRKKLNGFVPERRVRAKPL
                     DVPADSEYTEFYKGSGAHAMSTTMAAVRLLTKLLRHEGIGKRIVPIIPDEARTFGMDS
                     LFRQIGIYSPKGQLYEPVDSETLLFYNEVKDGQIIEEGITEAGAMSSFIAAGTAYANL
                     GVNMIPFYFYYSMFGFQRIGDLIWAAADNKSKGFLLGATAGRTTLNGEGLQHQDGHSH
                     LLASTVPTLLTYDAAFAYEIAVIIKDGMRRLYAEGEESFYYLTLYNESYEMPAMPEGA
                     EEGILKGLYKVKPATKGKKYKAHLFGSGPILREALRAQEVLAEQFDVSADVWSATSYK
                     LLRGDALRTKRWNMLHPTAAPKQSYLESILQNEEGVFVAVSDYMKMVPDQIAPWVPGG
                     LMTLGTDGFGRSDTRANLRRFFEVDAELAVLATLYALNQKEAFPAKVVEDAIRKLGID
                     PEKSFPFYR"
     gene            2116..3435
                     /locus_tag="VMZ02_08315"
     CDS             2116..3435
                     /locus_tag="VMZ02_08315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HUR71977.1"
                     /translation="MDVKLPQLAEGVEGGTVVSILVSEGQTIEKGQAFMELETQKAVG
                     SIPAPSAGVVTKIHVTQGMEVSVGQMLISMDTPGAKAPAPAAPRQADKSVEASVTPAP
                     ERSSESVPNFAHAASATPVGDYRYESKSGMAPPASPAIRKLARDLDIDLTRVRGSEAG
                     GRITLADLRGYVQKLQQLAFQATPTAAAAPAPAANPVEAIDFAKWGPIRREKMSPLRR
                     TVSRRMVESWTSIPKINQFADADVSALLALRKKYAPAYEKNGAHLTLTSFILFILGRA
                     LRKHSRANASMDEQSLEIVYKDYCHIGVAVDTEAGLIVPVLRDADKKNLLQLSSDLNA
                     LTEKTRQRKIAIEELQGGSFTISNQGSIGGSHFTPIIYAPQVAILGIGQGQPKPVAVE
                     GKIAIRTILPLCLAYDHRVLDGADAVRFLKDVIAGLESFDEGDIKLK"
     gene            3447..>4127
                     /locus_tag="VMZ02_08320"
     CDS             3447..>4127
                     /locus_tag="VMZ02_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR71978.1"
                     /translation="MEPITTEILVLGAGPGGYAAAFYAADKGKKVALVEQSPRLGGIC
                     LNVGCIPSKALLHATEILRESKESAARGIALGEAQINLEKLRSWKTGILDKLGQGIKT
                     LAEQRKVQVIHGRGHFEDSQTLRVETPEGQKFIRYEKAIIAVGSKPAMPNAFDLGNPR
                     VMTSTGALEIPDIPENLLVVGGGYIGMELGTVYAALGSHVVVLEALPSILAGVDADLV
                     RPVTRAAQK"
BASE COUNT          994 a         1183 c         1138 g          812 t
ORIGIN      
        1 ggcgccgatc atgtcgattt accacgcaag attcctgcgc tacctgcaaa gccgcgacct
       61 gattagcggc gaggagccgc gaatattttg tttcgccggc gacggcgaga tggatgaacc
      121 ggaatcttcc ggtgccttga ctttggcgtc acgggagaac ctagacaact tggtttggat
      181 cgttaactgc aacctccaac gcctcgatgg tcccgtgcgc ggcaacggca aaatcattca
      241 agaactcgaa gcgttatttc gcggcgccgg ctggaatgtc atcaaggtca tctggggctc
      301 agactgggac gacctgctcg ccaaggacga taagggcctg ttgcgcaagc gcatggaaga
      361 agccgtcgac ggcgattacc agaaatattc agtggagccc ggtagctaca cgagaaaaca
      421 ttttttcggc aagtatcccg aactgctcga gatggtcaat cacatgaccg acgatcagat
      481 ccacaagctc ctgcgcggtg gccatgatcc gatgaaggtg tatgccgcct ataaagccgc
      541 cgtggagcac agaggcgcgc ccaccgtgat cttggcaaaa accgtcaaag gttatggact
      601 cggcgaagcc ggcgaaggcc gcaacattac gcaccagcaa aaaaagctca acgaacgaga
      661 gctgcgtgag tttcgtacac gcttcgatat tccgctgccg gatgaagaag ttgtcgagac
      721 gcccttttat cgtccgccgc tcgatagccc ggagacgcaa tatttgttgg agcggcgcaa
      781 gaaactgaac ggattcgttc cggagcgcag agttcgcgcc aaaccgttag acgttcccgc
      841 cgattccgag tacaccgagt tctacaaagg cagcggcgcc cacgcgatgt cgacgaccat
      901 ggcagcggtc cggttgctta ccaagcttct tcgtcatgag ggaataggca agcgtatcgt
      961 gccgattatt cccgacgaag cgcgcacttt cggcatggat tcgttatttc gtcagatcgg
     1021 gatctattcc ccaaaaggcc agctctacga accggtcgac agcgagacgc tactttttta
     1081 caacgaagtg aaagacggtc agattatcga agaaggaatc accgaagccg gcgccatgtc
     1141 ttcgttcata gcagccggca ccgcctacgc caatctcggc gtcaatatga ttccctttta
     1201 cttttattat tcaatgttcg gcttccagcg tatcggcgat ttaatttggg ccgctgccga
     1261 caacaagagc aaaggctttt tgcttggcgc gaccgcgggc cggactactt tgaacggcga
     1321 agggctgcag catcaagatg ggcatagcca tctgctggcg agcacggtcc cgacgctgct
     1381 tacctacgac gccgctttcg cgtatgagat agccgtgatc atcaaagatg gcatgcgccg
     1441 gctttacgcc gaaggtgaag aaagttttta ctatctgacg ctctataacg agagctacga
     1501 gatgccggca atgccggaag gcgccgaaga aggcatcctc aaaggcttat acaaagtgaa
     1561 gcccgccacc aagggaaaaa aatataaagc ccatctattc gggagcgggc cgattttgcg
     1621 tgaagccttg cgcgcgcaag aagtgctggc cgaacaattc gacgtcagcg ccgatgtttg
     1681 gagtgcgacg agttataagc tcttgcgcgg cgatgccctg cgcaccaaac gctggaatat
     1741 gctccatccg acggcagcac cgaaacaatc ctatctcgaa tcgattttgc agaacgaaga
     1801 aggcgtgttt gttgcggtgt ccgattacat gaagatggta cctgatcaaa ttgccccctg
     1861 ggtgcccggc gggctaatga ctttgggcac cgacggcttc ggacgcagcg acacccgcgc
     1921 caacctccga cgatttttcg aagtggacgc cgaattggcg gtgctcgcga cactttatgc
     1981 actgaaccaa aaagaggcct ttccagccaa agtcgtcgag gacgcaatcc gtaagctcgg
     2041 catcgatccg gaaaagtcct tcccctttta ccgctaaaca atccccatca catttacagc
     2101 agcggcacat tcttcatgga cgtaaaactt ccccaactcg cagaaggcgt agagggcggc
     2161 acggtggtca gcatcctggt cagcgaaggc cagacaatcg aaaagggcca ggctttcatg
     2221 gagctggaaa cccaaaaagc cgtagggtcg attccagcgc cgagtgccgg cgtcgtcacc
     2281 aaaattcatg tcacccaagg catggaagtg agcgtcggtc agatgctcat ctccatggat
     2341 accccgggcg ctaaagcacc cgcacctgca gctccccgcc aagcagataa aagcgttgaa
     2401 gcaagtgtca ctccggctcc ggaacggtca agtgaatcgg tgccgaactt cgcccacgca
     2461 gcgtctgcta caccggttgg cgactatcgc tatgaatcga agagcggaat ggcaccgccg
     2521 gcgtcaccgg caattcgcaa actggcccgg gacctcgaca tcgacctgac cagggttcgc
     2581 ggaagcgaag ctggcggacg catcacttta gccgacctcc ggggttatgt tcaaaaactc
     2641 caacagctag cctttcaagc cacgccgaca gcggccgctg cgccagcgcc ggctgcaaat
     2701 ccagtggaag ccatcgattt cgccaagtgg ggaccgattc gacgcgagaa aatgtctccc
     2761 ctacggcgaa cggtgagccg gcgcatggtc gagtcttgga cttcgattcc caagatcaat
     2821 caatttgccg atgccgacgt tagcgcactg cttgccctgc gcaaaaaata tgcgccggca
     2881 tacgagaaaa atggtgcgca tcttactttg acgtcgttta ttttatttat tctcgggcgc
     2941 gcgctcagga agcattcccg cgctaacgcg agcatggacg aacaatcgct ggaaatagtc
     3001 tataaggact actgtcacat cggcgtggcc gtcgacaccg aggcggggtt gatcgtcccg
     3061 gtgctgcgcg acgccgacaa aaagaatctg cttcaactct cgtcggatct gaatgccctg
     3121 accgagaaga cccgccaacg aaagatcgcc atcgaagaac tacaaggcgg cagcttcaca
     3181 atttccaatc aaggcagcat cggcggcagc cactttacac cgatcatcta cgcgccgcag
     3241 gtggccattt tgggcatcgg tcaagggcaa cccaagccgg tcgcagtcga gggcaagatc
     3301 gccatccgca ccatcctgcc actctgtctg gcgtatgatc atcgagtgct cgacggtgcc
     3361 gacgccgtgc gcttcctgaa agatgttatc gccggcctgg aatctttcga cgaaggcgac
     3421 atcaaattga aatagattga agcgctatgg aaccgatcac gaccgaaatt ttggtgttag
     3481 gcgccggccc gggcggctac gccgcggcgt tttacgctgc cgacaaaggt aaaaaagtgg
     3541 cgctcgtaga acagagcccc cggcttggcg gaatttgttt gaacgtcggc tgtattccct
     3601 cgaaagcctt actccacgct acggaaatcc tacgcgagag caaagaatcc gccgctcgcg
     3661 gcattgcctt gggtgaagcg caaatcaatc tcgagaagtt gcgaagttgg aagacaggca
     3721 ttctcgacaa gctcggccaa ggcatcaaaa ccctcgccga acaacgcaag gtccaggtca
     3781 tccatggccg cggtcacttc gaggactcgc agaccctgcg ggtggaaacc ccggaaggac
     3841 aaaagtttat ccgctatgaa aaggcgatca tcgccgtcgg ctccaagccg gccatgccca
     3901 acgctttcga tctcggcaat ccaagagtca tgacctcgac cggagccttg gagatccccg
     3961 acattccgga aaatctactg gttgtcggcg gcggttatat cggcatggag ctcggcacag
     4021 tctacgccgc actgggcagt cacgtcgtcg tcctggaagc gctaccgtcg attcttgccg
     4081 gcgtcgacgc tgatcttgtc aggcctgtga cacgcgcggc ccagaag
//
LOCUS       DATLCH010000284         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7812659, whole genome shotgun sequence.
ACCESSION   DATLCH010000284 DATLCH010000000
VERSION     DATLCH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7812659"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VMZ02_08325"
     CDS             complement(<1..219)
                     /locus_tag="VMZ02_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HUR71979.1"
                     /translation="MAMDASSQRDGTTIAQINVTPLVDVMLVLLVIFMVTAPIIQQGV
                     QVNLPTTSSSAIPGTEEHLVVTVAKNGKI"
     gene            complement(294..1034)
                     /gene="tolQ"
                     /locus_tag="VMZ02_08330"
     CDS             complement(294..1034)
                     /gene="tolQ"
                     /locus_tag="VMZ02_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HUR71980.1"
                     /translation="MAFPWSLKLAVQNALPAPHQQGILDLVRGSGLVVQAILYILVFF
                     SVMSWGIIAQKLQQIRRAKKESAKFIEIFWERRNLSSIHDASRELASSPVGQVFRAGY
                     EELVRVTRSKKESAPGENLTTELGGVENVSRAMKRATSVEITKLEKYCSFLATTASSA
                     PFVGLFGTVWGIMDAFRGLSVTHSSSIQAVAPGIAEALIATAVGLAAAIPALIAYNYF
                     VQQIKVLAVEMDNFSHEFLNIAERHFFK"
     gene            1136..2314
                     /locus_tag="VMZ02_08335"
     CDS             1136..2314
                     /locus_tag="VMZ02_08335"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HUR71981.1"
                     /translation="MIRKLADLDLRGKTVFLRVDFNVPIKDGKVGEPHRIESALPTIQ
                     FLLGRATKVIIASHLGRPDGKVVDKYSLVPVQRHLQDALKLPVTMASDCVGPAVEKLV
                     RATDNQIVLLENLRFHPEEEKNDEAFCRALAALADVYVNDAFGAAHRAHASIAGMAKY
                     FRDKAAGLLLQKELDYLGPLLADPAKPFVTIMGGAKVSDKIDVIKNLLPKVSSLLIGG
                     GMAYTFLKASGIEVGKSLVETDKIALAAEIMQQAKQLGVELLLPVDHVAGDSQKKNPV
                     TFDGAIPTDRMGLDIGPKTAHIFVEQIKKAKLVLWNGPVGLFEIVPYDQGSRLLAEAL
                     AQSHPAVTGIIAGGDTVAAVSAAGVSARIAHLSTGGGATLEFLEGKVLPGIAALDDRP
                     "
     gene            2311..>2615
                     /locus_tag="VMZ02_08340"
     CDS             2311..>2615
                     /locus_tag="VMZ02_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HUR71982.1"
                     /translation="MTSPLIVANWKMHGDQAQCVRLTRQIVRALSRDPVNAEVVLAPP
                     FTSLKSAHDSLAGSKVKLAAQNCHWEIQGAYTGEISPMMVRELGCDYVIVGHSERRH"
BASE COUNT          646 a          703 c          681 g          585 t
ORIGIN      
        1 gatctttccg ttcttggcaa cggtcaccac cagatgctcc tcggtcccag gaatggcact
       61 actgctagtc gtgggcaaat tgacctgtac tccctgctgg ataatcggcg ccgtcaccat
      121 aaagattacc aacaacacca acatgacgtc gaccaacggg gtgacattga tttgcgcaat
      181 tgttgttccg tcacgctgcg acgatgcatc catagccata ccgtgttccc aatcctgcta
      241 cggcccagag gactgggccg cacccgctgt tgagattata cgctttaatt gtgctacttg
      301 aagaagtggc gctcggcgat gttgagaaat tcgtgagaaa agttgtccat ctctacggct
      361 agcactttga tttgctgaac gaagtaatta tacgcgatca gtgcagggat cgccgcggct
      421 aaaccgaccg ctgtggctat caatgcttcg gcgatgcccg gagccaccgc ttggatgctc
      481 gaagagtggg tcacgcttaa accgcggaaa gcatccatga tgccccacac cgtaccgaac
      541 aaaccgacga acggcgcgga gcttgccgtt gtcgccaaga acgagcagta tttttccagt
      601 ttggtgatct caacgctggt cgcccgtttc atcgcccgcg aaacgttctc gaccccgcca
      661 agttccgtcg taagattttc gcccggtgca gattcttttt tggaacgtgt gaccctaact
      721 agctcttcat aaccggcgcg aaaaacttgg ccaaccgggc tcgacgccaa ctcgcggcta
      781 gcatcgtgaa tcgatgataa attgcgccgc tcccaaaaaa tttcaataaa tttcgccgac
      841 tcttttttgg cgcgccgtat ctgctgaagt ttctgcgcga tgatacccca gctcataacg
      901 gagaaaaaaa ccaatatgta taaaatcgcc tggacgacca gtccggaacc ccgtaccaag
      961 tcgagaattc cttgctgatg cggcgcaggt aaagcgtttt gaactgcgag tttaagcgac
     1021 cagggaaaag ccattacgtt tgctgcggat aattgaaaaa ctattacaag ccttgtaatt
     1081 agtcaattat aggaagcctt ttattaagaa atatttgcac agcggatggc aaacgatgat
     1141 tcgaaaactc gcggaccttg acctacgtgg caagactgtt tttctgcgcg tcgactttaa
     1201 tgtgccgatt aaagacggca aagtaggcga acctcatcgc atcgagagcg cactgccgac
     1261 gatccagttt ctcttaggtc gcgccacgaa ggttataatc gcgtcacacc tcggtcgccc
     1321 cgacggcaaa gtcgtcgaca aatacagtct cgtgccggta cagcgtcacc tgcaagacgc
     1381 tcttaaactt ccggtgacta tggcatccga ctgcgtcggt ccggcggttg agaagttggt
     1441 gcgggcgacc gacaaccaaa ttgtcctttt agaaaacctc cgctttcatc cagaagagga
     1501 aaaaaatgat gaagcctttt gccgcgcgtt ggcagccctc gccgatgtat acgtcaacga
     1561 cgcgttcggc gcggcacata gagcccatgc gtcgattgcc ggaatggcta aatacttcag
     1621 agataaggcc gccggccttt tgttgcagaa agagctggac tacttgggac cgctgttggc
     1681 agacccggca aagccgttcg ttacaatcat gggcggcgcc aaagtatccg acaaaatcga
     1741 tgtcatcaaa aacttgcttc ctaaagtcag ctcgctcttg attggcggcg gcatggccta
     1801 cacctttcta aaggccagcg gaatcgaagt cggcaaatcg ctcgtggaaa ctgacaaaat
     1861 cgcactagcc gcagagatca tgcaacaagc caagcagctc ggtgttgaat tactgttgcc
     1921 ggtcgatcat gtagccggag acagccagaa gaaaaatcct gttacttttg acggtgcaat
     1981 accaaccgat cgcatggggc tcgatatcgg accgaaaacg gcgcacattt ttgtcgaaca
     2041 gatcaagaaa gccaaacttg ttctctggaa tgggccggtg ggacttttcg agatagtgcc
     2101 gtacgatcag gggagccgac tgctggcaga ggcgctagcc caaagtcatc cagcagttac
     2161 cgggataatt gccggcggcg acacggtagc agcggtgtcg gcagcggggg taagtgcgcg
     2221 cattgcccac ctctctacgg gcgggggcgc aactttagaa tttcttgaag gcaaggtatt
     2281 gcccggcatt gccgcactgg acgaccggcc atgacttccc cgctcattgt cgctaactgg
     2341 aaaatgcatg gcgatcaggc tcaatgtgta cgtctaaccc ggcaaatagt ccgagcacta
     2401 agtcgtgatc cagtcaacgc cgaagttgta ctggctccgc cgttcacttc tttgaaatcg
     2461 gcacacgact cgctcgccgg cagcaaagta aaactcgctg cgcaaaattg ccactgggaa
     2521 attcaaggcg cctataccgg cgagattagt ccgatgatgg tccgcgagct tgggtgcgat
     2581 tatgtcattg ttggccattc ggagcggcgc cacat
//
LOCUS       DATLCH010000285         3328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2976717, whole genome shotgun sequence.
ACCESSION   DATLCH010000285 DATLCH010000000
VERSION     DATLCH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2976717"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VMZ02_08345"
     CDS             <1..452
                     /locus_tag="VMZ02_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR71983.1"
                     /translation="LYRASQAGVEIDLIVRGMCSLRPGLPGLSERIRVVSIIDRYLEH
                     ARIFYFQNHGKPEYFLASADWMQRNFDRRVEIAFPVLDAQYQTKLKEILETQLADNVK
                     GWRIQSDGSSVRMHSDGANSMRSQQRFYEIMDTERSGSAPRTTHEID"
     gene            complement(923..2182)
                     /locus_tag="VMZ02_08350"
     CDS             complement(923..2182)
                     /locus_tag="VMZ02_08350"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR71984.1"
                     /translation="MSEDFTQSPQAEVRLAPWASLRYRDYSLLFLLSLCATTAQQMRQ
                     TQNFYQVYELSGSAFQLGITGVAQGVPIFVFGLFGGTLADFVDRKKLILLTTLGNFLV
                     AATLGVLTLTGAIQVWHIQAGTAMTSALNIVLNPTRMSLISYLVPRSHLTNAVSLNSA
                     ISQGSHFIGPMLGGLSLAWMSTGNAYLFNALFYLPAVFAIVSLTIPASAKPSHEKLAF
                     ASLLGGVKFLFSEPVVLAMVLLDFVIVGVGYYRPLLPIFAKDILFVGPAGFGMLSSAP
                     AVGGMIGTVALLVIRDITSKGMLALWSFLAYALALGAFALSTKLWLSLGLLGVMGMAN
                     SLQAVMRQTSFHLLTPQAVRGRAFAVFHMFSQGANSVGATEVGFMAALIGAPGSLLFG
                     CVVGTLLTLGYWIGMPRLRNFRAEKVL"
     gene            complement(2206..>3328)
                     /gene="larC"
                     /locus_tag="VMZ02_08355"
     CDS             complement(2206..>3328)
                     /gene="larC"
                     /locus_tag="VMZ02_08355"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HUR71985.1"
                     /translation="GDMFVAAMIDLGLPLGKLKAELKKIPTLEFTLKAAQKSVHAVRA
                     TQFQVICPRGESARSWPQIRRLLERSKLSSTIKATSVDIFTRLAKAEAKIHGVDVEQV
                     HFHEVGATDSIVDIVAAAIGVNELAIDELHFSRIPLGRSITGSQHGPLPVPAPATIEL
                     LKGLPTFGIDLESETVTPTGAALIKTLGSTFGDQPSMTIDKIGYGTGQKEFSNRPNLF
                     RLLIGESAGRLQQDQMLVIETNIDDMNPQHFDHVMERLFTAGARDVFLTPTQMKKNRP
                     ATLLTAICEIPLRDRLAAIVLQETSSIGLRYYPVNRILLRREVKSVKTRYGEVRVKIV
                     TQPDGTERATPEYDDLKRIATAEDTTLKTIHDEVMRHLN"
BASE COUNT          778 a          894 c          963 g          693 t
ORIGIN      
        1 agttgtatcg cgccagccaa gccggtgtcg aaatagatct cattgtcagg ggaatgtgca
       61 gtctacggcc cgggctgccg ggactatcgg agcgcattcg cgtggtttcg attatcgacc
      121 gctatctcga gcatgcgcgc atattttatt tccaaaatca tggcaagccg gaatatttcc
      181 tggcatcggc cgactggatg cagcgcaatt tcgatcgtcg agtcgaaatc gccttcccag
      241 tattggacgc gcagtatcag acaaaactaa aagagatctt ggagacgcag ctagccgaca
      301 atgtgaaagg ctggcgcatt cagtccgacg gcagttcggt acggatgcac agcgatggcg
      361 caaactcgat gcgctcgcaa caacggttct acgagatcat ggacaccgag cgcagtggat
      421 cggcaccgcg aacgactcat gagatcgatt gatgccgaga gttggtaggg gttcagtttg
      481 aggctaaaga atccgccccg ctgcgttgac aaaactacgt ccaatctatt tgatcattct
      541 tagccgtaat tcattagcgg gaggtgcctc atgaccgact gcctgcatga aaaaacaact
      601 gcgcgaggct ccgagcaggg tatattttgc cgcgactgcg gcgaaaaact ttatgaactt
      661 gagcctcgtg aatgccagta ttgtgtgagt tttcgcccta ctctaccgtt caacacatgt
      721 cacaagaaaa ccgagtccgt gtttcgaaac atgcacgccc tattcaaagt aggtgaggga
      781 acgtgcttcg acgagaacaa gtcagactga accacgaccc gagagttcgc catattgtta
      841 tatccatcac gacgccgcga gagtgcggta ggaccaggtt cgttatagtg gcgggttagc
      901 ccaacgccgc ccggcgcatt cactaaagca ccttctcagc cctgaaattt cttaacctcg
      961 gcatgccgat ccagtatccc aacgtcaata acgtcccgac gacgcagccg aacagcaacg
     1021 agcccggtgc gccgatgagc gccgccatga atcccacttc cgtcgcgccg acgctgttgg
     1081 caccttgaga aaacatatga aaaacggcga aggcacggcc gcgaactgct tgcggcgtga
     1141 gcaagtgaaa tgatgtctgg cgcatgaccg cttggagcga gttcgccatc cccatgaccc
     1201 cgagaagtcc cagcgaaagc caaagctttg tcgatagggc gaaagcgcca agcgctagag
     1261 cataggcgag aaatgaccaa agggcgagca tccccttgct tgttatatcg cgtatgacga
     1321 gcagcgcgac ggtgccgatc atgccgccga ctgccggcgc tgaagacaac atgccgaaac
     1381 cggcgggccc cacgaacaga atatctttag cgaagatcgg caagagcggc cgataatagc
     1441 cgacaccaac aatgacgaag tcgagcagta ccatggcgag taccaccggc tcggaaaaaa
     1501 ggaattttac accaccgagc aaactggcga aggcaagctt ctcgtgactc ggtttagcgc
     1561 tggcgggaat cgttagtgag acaatggcga agaccgccgg cagataaaaa agcgcgttaa
     1621 acaaataggc attgccggtg ctcatccacg ccagacttaa gcccccgagc atggggccga
     1681 taaagtgcga gccttgcgag attgccgagt tgagcgaaac cgcgttggtg agatggcttc
     1741 gcggcaccag gtaagatatc agcgacatgc gcgtcggatt gaggactata ttgagagccg
     1801 acgtcatcgc cgtgccggcc tgaatatgcc agacttggat cgctccggtg agggttaata
     1861 cgccgagcgt ggcggccacg aggaaatttc ccaaggtcgt cagcagtatc agtttcttgc
     1921 ggtcgacaaa gtcggcgagt gtgccgccaa acaatccgaa aacaaagatc ggcacaccct
     1981 gggcgacgcc ggtgatgccc aactgaaacg ccgagccgga aagctcgtag acctgataaa
     2041 aattctgtgt ctggcgcatc tgctgagcgg tggtggcgca caaagatagc aggaacagca
     2101 aactgtaatc gcggtaacgc agcgaggccc aaggcgcgag ccgcacttcg gcttgcggcg
     2161 attgtgtgaa atcttccgac atgatgtggc cgcaaaaagt ctcggctagt tgagatgccg
     2221 catcacttcg tcgtgaatag ttttgagagt cgtatcttcg gccgtggcta tgcgcttgag
     2281 gtcgtcgtat tcgggtgtcg cgcgctcggt gccgtccggt tgggtgacga ttttgacccg
     2341 cacctcgcca tagcgagttt tcacgctctt cacctctcgt ctcaacagaa tgcggttcac
     2401 gggatagtat cgcaaaccga tgctgctggt ctcctgcaag acgatcgcgg caagccggtc
     2461 gcgtaggggg atttcacaaa tggccgtcag cagcgttgcc gggcgattct ttttcatctg
     2521 ggtcggtgtg agaaagacgt cgcgagcccc ggcggtgaac agacgctcca tgacatgatc
     2581 gaaatgctgc ggattcatat cgtcgatatt ggtctcgatg accaacatct gatcctgctg
     2641 caagcggccg gcgctttcgc cgatgagcaa acgaaagagg ttgggacggt tcgaaaattc
     2701 tttctgcccg gtgccgtagc caattttatc gatcgtcatg ctcggctgat caccgaaggt
     2761 gctccccagg gtttttatta gcgctgcgcc ggtgggcgtc accgtttcgc tctctaagtc
     2821 gatgccgaag gtcggcagac cttttaacaa ttcgattgtc gccggtgcgg gcaccggcag
     2881 cgggccatgc tgcgaaccgg tgatgctgcg acccagggga attctcgaga aatgcagctc
     2941 gtcgatcgcc aattcgttta caccgatggc cgcggcgacg atgtcgacaa tggaatcagt
     3001 ggcgccgact tcgtgaaagt gcacttgctc gacgtctact ccatgaatct tcgcttccgc
     3061 tttggctagg cgagtgaaaa tgtcgacgct tgtcgccttg atggtggagc ttagtttgct
     3121 gcgctcgagc aggcggcgaa tctgtggcca cgagcgcgcg gattccccgc gtgggcagat
     3181 gacctggaat tgagtggctc ggacagcatg gacagacttc tgcgctgcct tgagagtgaa
     3241 ctccagggtc gggattttct tgagttccgc tttgagtttt cccagcggca accccagatc
     3301 gatcatggcg gcgacgaaca tatcgccg
//
LOCUS       DATLCH010000286        11604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3367963, whole genome shotgun sequence.
ACCESSION   DATLCH010000286 DATLCH010000000
VERSION     DATLCH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11604
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3367963"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1407
                     /locus_tag="VMZ02_08360"
     CDS             301..1407
                     /locus_tag="VMZ02_08360"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HUR71986.1"
                     /translation="MIKISTFDDWIDYFHQWQTDIGYDAALLGDYKFETKLGELHSPE
                     IEFGDFKGQPKWQRIGQIPNQSIRDALMNLIVYQGDTEFASVEQQKNLLDTAPTEYDR
                     KALTRVNSEEMRHGWQMCYLLVNYFGDSGKLEARKLLERRAFRGDRLLGSFNAPVNNW
                     LDFFTYTEFVDRDGKYQLTMLSHSSFAPLAESVTAMLKEEHFHMFTGHTGLTRVLRAK
                     KIPVPIVQKYFNKWLSTAYDLFGTDHSSSAHWAYTWGIKGRYDEHAATVPAEKDKLND
                     IAREHYMKESQQLIDQLNQLVPEGEAKLRAPDLKFNRSIGEHVGKTYSVTGELLGTEE
                     YHKHLYNTLPNAEDEKILAEIITGKDWVSQMQLN"
     gene            1471..1794
                     /locus_tag="VMZ02_08365"
     CDS             1471..1794
                     /locus_tag="VMZ02_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HUR71987.1"
                     /translation="MSQSCLVCQNIDCKSRGSDQLAAELQQKIAAKGLTDIEVKPYMC
                     FGACQEGPNVVIYPEKNWYAHVKSEDVDDIVAHLAGGPHVQRLDTIDSSLKELIYQLL
                     DTGVF"
     gene            1799..3049
                     /locus_tag="VMZ02_08370"
     CDS             1799..3049
                     /locus_tag="VMZ02_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HUR71988.1"
                     /translation="MEGILFPKGVIEGREGLTEYQARGGYEALANALKGTPEDVIQIV
                     SDAGLRGHGGAGFPTGKKWQFTREAPEQPHYLVLNGGEDEPGSRKDRVLLENLPHLVI
                     EGTVLGAYAIAASKAYLYINAKYETAIKSITDALTEAKAAAYWGDNILGSSYNLDIEI
                     IAAPHNYVAGEDTAVLEVIEGKKPLPRQKPPFPVTVGLFGKPTSVNNVETLANVPRIL
                     LKGADWYRSFGTKECPGTMLFSLNETEDVNRPGIYELPFGTPLRYLIEECGGGLKNGK
                     TIKAIMPGSPSSAYLRADKIDTPLEPNAMREAGSMLGCGVVHIVREGDSIVDELVRIT
                     GFFAAESCGQCAACRMETNTLSMLMKKIQQGQGNLALLEQFGKVLAFNKGKGFCNLIA
                     MPGPPIESALKLFPADFEALLPTT"
     gene            3186..4199
                     /locus_tag="VMZ02_08375"
     CDS             3186..4199
                     /locus_tag="VMZ02_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71989.1"
                     /translation="MPKMTNFALSFLFGFLFATPVSANNHQPLIDGAKKEGSLVLYTS
                     MTVDQAQKLNDAFRAKYPFLQVQMFRAVGERLLTKIMTETQAGRFDFDVVQSAETQAY
                     FLKKRNLLAKYLSPETKQVQKGFFDADGFWAAIYMMPNVIAYNTRMVKRSEVPLTDDD
                     LLNPKWRGKIGMDGTKPEWFSWKLKRLGQEKGLSYMKRLGAQDFRLYSGLTIITGLLA
                     AGEFPLVLNTYVHNAEDAKRKGAPVDWVAQDPVFTKFQPIGVGAKAPHPNAAKLFVDF
                     MLSEEGQRIIASFGRVPTRRAVATNVQGLDKLNFVIDEFSAGDDFNKSYEQFRNVFGA
                     PKL"
     gene            4210..5043
                     /locus_tag="VMZ02_08380"
     CDS             4210..5043
                     /locus_tag="VMZ02_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HUR71990.1"
                     /translation="MRLAPIKWPNNAKIAVSFFVAFEAFEKSSQYRRSEGKPDYASLA
                     YGEYGGKAGIWRIMDVFARNGVKGTIDTNGRIAEDFPDALQELHGAGHEIVGHGWAND
                     LALSDVENAASERALIKRTLDALASVTGKRPVGWVSPGHRINDYTFGNFVDEGIFWTG
                     DLPGPDIPQHRIVNGKPLIVMPRLDYANDLSSIFTPKNPPRVYFDSFKAAFDCLYAEG
                     QAGAPKLIDCLVHAHIGGRPHLIGVWEECIRYAKGFPDVWFATKGEIAKYYLDTYAAK
                     S"
     gene            5058..6236
                     /locus_tag="VMZ02_08385"
     CDS             5058..6236
                     /locus_tag="VMZ02_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUR71991.1"
                     /translation="MARAIENYLAGLKSDQLLLQFTWDEWLAIIPPYPDREFTSADVT
                     VRYQRNGYDWDMHGTLYTPAQETDPRRALVFFHGGAGSEKIMDLTPDGRPGLARVAAA
                     QGFKVLALTYPGHYPPGGVWKIPIPERMPIYLLDRQLNQEEIADRNLKCTFNVILQGA
                     GALTDQHLAGREIIGFGHSTGGPMAAHLTRFSKKTKVIGLVGFGSGGPDGWRKEWRDQ
                     TGAEKYREIPLDHVSRRSPDSFKNSGYVDPEDLTPWGGADDYIRLVSPLRSQMKTSLC
                     DNQHNAAIGILEEYPKRTGLPREEYFDHLQEPDANWLRSIRVLLVAGENDKGHWVNGG
                     APLENKREMFMGRKYAATTRGAHVVFVPRYGHVGYSELYNEKIAYLWLWAYQSGYFDN
                     "
     gene            6261..7328
                     /locus_tag="VMZ02_08390"
     CDS             6261..7328
                     /locus_tag="VMZ02_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71992.1"
                     /translation="MLTRVMLCFLLSNLVPVELTFGAQAKPMSPAELALYQGKDREQL
                     IVDGAKKEGQMTFYTSNTWMAGHVSQEFGKKYPFIKPNIYRSDSKELIKRLTEEAGAG
                     RQLADVAETGPEYMALMHSTGMIQEVLLPELTRYDEEAKVKGKKGVLYWISRELYISV
                     GYNTKLVAPGDVPKSMKDLLDPKWKGKMTIAGTTTGTQWIGAIIETFGMEFLEKLAAQ
                     NINVQNISGAALAELVVSGEVPLSPTIFNSNISTAKQRGAPVEWKPIDPVIGTTGMSG
                     LVAKAPHVHAGLLFLDFLHSKEGQQAVIKGGLSSARNDIDDADMKFKKLYIESKYPPD
                     VIEKKFGEWDGLKRKLFISKR"
     gene            7336..8370
                     /locus_tag="VMZ02_08395"
     CDS             7336..8370
                     /locus_tag="VMZ02_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71993.1"
                     /translation="MSPSAHFGRLLAVALFCYSTAAGNEAQAAEKSTKSAVKIRISQS
                     AVGARSTILWSAQELGLFAKHGIDAEVVYLRSSPIQMTALSTGEVQFAASGGSPVLGA
                     AAAGQDIKIVASPSNRLSYDLVVRPEIKEAKDLRGKRFGITSIGGTVWMAAYLALEHL
                     GLDANRDQIRINAIGNQTILAQAVESAQIDATLLDPFLSRQTKAKGLPVLIELQRAQI
                     PFTNTAWAVSGSFLRERADAVEAVLKAMFEAQAFITAPANRNAVLQIMARHMKLNDAV
                     LLQDGYQDLLVGLERKPYAALDGLRNIQRIMSTINANASRAKVEEIIDNRIVRKLDES
                     GFINGLYAKR"
     gene            8416..9360
                     /locus_tag="VMZ02_08400"
     CDS             8416..9360
                     /locus_tag="VMZ02_08400"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR71994.1"
                     /translation="MLPTVLITGANGFIGTHFTKLMESAGHKVIPIDVVARAPDLSLL
                     GVKATSHLMNVTDGAAFRALCEKERPTHLFHAAHPPRDETPTVLDYCYRAMTNILEAA
                     RDLKMQRVVYSSSASIYGQLQKGAGGLVKEDDAITIYPTYFYRAAKTVSEWMGNFYKE
                     KHNVDFVALRYSSVYGPGLYRSIPLELKKGILGQPCRPFLTRPLDDLIYVDDVVDAIS
                     RALFAQGPLSKAYNVGLDKAYVSDDLKRAIEKALPNLDFEIGKHPNAAEVAPHRLRSP
                     LDISLARKELGWQPKIYLEDGIARLAAWLKTHKADLSL"
     gene            9680..10735
                     /locus_tag="VMZ02_08405"
     CDS             9680..10735
                     /locus_tag="VMZ02_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR71995.1"
                     /translation="MSRKFAFALIAVLISTQGFAANAPSSVAQIALYQGGDREKILIE
                     GARREGQVTFYNSHTWFRTFVKDFEKKYPFIKVAEWRNDSKNILRRVTDETKSGRGLA
                     DVIETTPEAMGILKRDGMLQEYFSPEARFFPDDVKSKGKNGFYYVGDRETYIGLGFNT
                     NSIPQTEAPKSLRDLLNPKWKGKMSIVTSSTGTRWIGNALDAMGREFVDKLAEQEVRL
                     QDLSGAALSGLVVTGEVPLSPTIFDANVSVHKQNNAPIDWRPLEPVVTNVGYSGISVK
                     APNPHAAMLLLDFIHSKEGQQLITKGGLWSPRDDIGTVEQRFKKSYLDEKYSIEELEV
                     KYGQWEQLIRRLFGQRK"
     gene            10775..>11604
                     /locus_tag="VMZ02_08410"
     CDS             10775..>11604
                     /locus_tag="VMZ02_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR71996.1"
                     /translation="MKSRPLPYPHTIIFNGKIRTFQTETSTCEALACSGSRIVAAGKL
                     EDLRRFAGPDTEMIDLKGRTAIPGLTDTHVHLSEKGTAEMELVDCRDFYVDVNSVSDI
                     LQRLANAAAKAPKGSWLVAHGSPMQDFRINDHRFPDKHDLDRAVPDHPVSISFGAHVT
                     IGNTLALAAAKITRDTADPAGGHIKHDPQTGEPTGELHERAQLILRKVAPEFNYLQLK
                     DGIVFALNQCLQRGVTTVHDIVRYAEPVRAYQELYKEGRMKARVSILPRVIESMIESK
                     A"
BASE COUNT         2896 a         3236 c         3057 g         2415 t
ORIGIN      
        1 ggctctgtat ctgccggtga ttaaaaaagc cattcgctca atcagttatc aggcggccaa
       61 aacgacggca cagatggttc tcgcgatgga taccgtcggt gagatcaaac gctatctctt
      121 tgaacaaatt cgcgaattgg aaatggtcga actgctagaa atgtatcgct aggccgaaga
      181 acctccctag cggttccagc cctttcaaat ttcaaagggc attgatttag cccgggcaaa
      241 ccgctataaa taattcgaac gatcgttcga actcacggct aaattgaaag ggaacctccc
      301 atgatcaaaa tctcaacttt cgatgactgg atcgactatt tccatcagtg gcagacagac
      361 atcggctacg atgcggcact gctgggtgat tataaattcg aaaccaaact gggcgaactt
      421 cattccccgg agatcgagtt cggtgatttc aaaggtcagc cgaagtggca gcgaatcgga
      481 caaatcccta atcaatcgat ccgcgatgcc ttgatgaacc tgatcgtcta ccagggcgat
      541 accgaattcg cttcggtcga gcagcaaaaa aatctactcg acaccgcccc caccgagtac
      601 gatcgcaaag cgctcacccg agtcaatagc gaagaaatgc gccatggctg gcagatgtgc
      661 tatctgctgg tgaattactt tggcgattcc ggcaagctcg aagcgcgcaa attgctcgag
      721 cggcgcgctt tccgcggtga ccgcttgctc ggctcgttca atgccccggt caacaactgg
      781 ctcgacttct tcacctacac ggagttcgtc gatcgcgacg gcaagtatca gttaaccatg
      841 ttgagccact cgtcctttgc gccgctcgcc gaaagcgtga cggcaatgct caaggaggag
      901 catttccata tgttcactgg gcataccggt ctgacgcggg tgctgagagc caaaaaaatt
      961 cccgtgccga tcgttcagaa atatttcaat aaatggcttt ccaccgccta cgatcttttc
     1021 ggcacggatc attcatcgtc agcacactgg gcttacacat ggggaatcaa aggccgctac
     1081 gacgaacatg ccgcaacggt gccggcggaa aaagacaagc tcaacgatat tgcccgcgaa
     1141 cattacatga aggagtcgca gcagttgatc gaccaactca atcaactggt gccggagggc
     1201 gaagcaaaat tgcgcgcgcc cgacctaaaa tttaatcgct ccatcggtga gcatgtcggc
     1261 aagacctata gcgtcacggg cgagttgctc gggaccgagg aatatcacaa gcatctctat
     1321 aacacgctgc cgaacgccga agacgagaaa atcctcgctg agataatcac cggcaaagac
     1381 tgggtctcgc aaatgcaact taactgagtt tcagtttctg ctgttgcata tcgaaccgag
     1441 cattagtaat taggaccgaa ggagttgccc gtgagtcaaa gctgtctcgt ttgtcagaat
     1501 atcgactgca agtcacgagg gtcggatcag ttggcggcgg agttgcaaca aaaaattgcc
     1561 gccaaaggcc taaccgatat cgaggtcaaa ccgtacatgt gctttggggc ctgtcaggaa
     1621 ggccccaacg tggtcatcta ccctgaaaag aactggtacg ctcacgtcaa aagcgaagac
     1681 gtcgacgaca ttgtcgcgca cctcgccggc ggtccccatg tgcagcgcct ggatacgatc
     1741 gattcgtccc tgaaggaatt gatctaccag ttactcgaca caggagtatt ctagaccaat
     1801 ggaaggcatc ctttttccta agggcgtgat agaaggccgc gaaggattga cagagtatca
     1861 ggcacgcggc ggctatgaag cattggccaa tgcgctcaag ggaactcccg aggatgtaat
     1921 tcagattgtt tctgacgctg gcttgcgcgg ccacggcggc gccggatttc ccactgggaa
     1981 aaaatggcag tttacccgcg aagcgcccga gcagccgcat tatctggtgc tgaatggcgg
     2041 cgaagacgaa ccggggagcc gaaaagaccg ggtgctgctg gaaaacctgc cacacctcgt
     2101 gatcgaaggt acggtgctcg gcgcttacgc catcgcggca agcaaagcct acctttacat
     2161 aaacgccaaa tacgaaactg cgattaagag cattactgac gctttgaccg aagccaaagc
     2221 ggctgcctat tggggcgaca atattcttgg cagcagttac aacctcgata ttgagatcat
     2281 cgccgcgccg cataactacg tcgctgggga agataccgcg gtactagaag taattgaagg
     2341 caagaaaccg ctgccgcggc aaaaaccgcc tttccccgtc accgtcggcc ttttcggcaa
     2401 accaacttcg gtgaacaatg tcgagactct ggctaacgtg ccgcgcattc tcttgaaagg
     2461 cgccgattgg tatcgcagct tcggcacaaa agaatgtccc ggcactatgt tgttttcgct
     2521 taatgaaacc gaggacgtca accggcccgg catctacgag ctgccgttcg gcactccgct
     2581 gcgctatctt atcgaagaat gcggcggcgg tctcaagaac ggcaaaacca tcaaagcaat
     2641 catgccgggc tcgccgtcct ctgcatacct gcgcgccgac aagatcgaca cgccgttgga
     2701 accgaacgcc atgcgtgaag ccggttctat gctcggctgc ggtgtcgtgc atatagtccg
     2761 cgagggcgat tccatcgtcg atgagttggt gcgcattacc ggcttcttcg ccgccgaatc
     2821 gtgcggccaa tgcgccgctt gtcgcatgga gaccaacacg ctgtcgatgc tgatgaaaaa
     2881 aattcaacag ggtcagggta acctggcgct cctcgaacag ttcggcaaag tgctggcatt
     2941 taacaaaggc aaggggtttt gcaacttgat tgccatgcct ggaccgccga ttgaaagtgc
     3001 gctcaagcta tttcccgccg acttcgaagc gctgctgcca accacctgat cgatacaacc
     3061 ggtttatcga attcaaagga tgccaagcgg catccttttt tttgcgccaa acttctccgc
     3121 aatcgtttga ccgtgtcgtt gacgccttga tagtatcccg cacgagtcaa gtcaggaaac
     3181 caagcatgcc caaaatgact aactttgcgc tctcgtttct gttcggtttt ttattcgcaa
     3241 cgccggttag tgccaataat catcagccac tcatcgacgg cgccaagaaa gaaggctctc
     3301 tcgttctcta cacatcgatg acggtggacc aggcacaaaa gctcaacgat gcattccgcg
     3361 ccaaatatcc cttcctgcaa gttcaaatgt ttcgtgccgt cggcgaacgc ctgctgacca
     3421 agatcatgac cgagacccaa gccggtcggt tcgactttga cgttgtccag tcggctgaaa
     3481 cccaagcgta ttttttgaag aaacgtaacc tattggcaaa atatctttca cccgaaacca
     3541 aacaggtgca aaaaggcttc tttgacgcag acggattttg ggcggcgatc tacatgatgc
     3601 cgaatgtcat cgcttacaat acccgcatgg tgaagcgcag tgaggtgccg cttaccgacg
     3661 atgacctgct caatccaaaa tggcgcggca aaatcggcat ggacggcacc aaaccggaat
     3721 ggttttcgtg gaaattgaaa cgactcggcc aggaaaaggg tctgagttac atgaagcggc
     3781 tcggcgctca ggattttcgc ctctattctg ggctgacgat catcaccggg ctactcgccg
     3841 ccggcgagtt tccactggtg ctcaacacct acgttcacaa cgccgaagac gcaaagcgca
     3901 aaggcgcgcc ggtcgactgg gtggcgcaag acccggtctt caccaagttt cagcccatcg
     3961 gcgtgggagc caaagcgccc caccccaacg ccgccaagtt gttcgtcgat ttcatgctgt
     4021 ccgaagaagg acaaagaatc attgcctcgt tcggccgagt gccgacgcgt cgggcagtag
     4081 cgaccaacgt gcagggattg gacaaactca attttgtcat tgacgagttc agtgccggcg
     4141 acgatttcaa caagagctat gaacagtttc gcaacgtttt cggcgcgcca aaactataaa
     4201 ggaaatatca tgagactcgc cccgatcaag tggcccaaca atgccaaaat cgccgtcagc
     4261 ttcttcgtag ccttcgaagc ttttgagaaa tcctcgcagt accggcgcag cgagggtaag
     4321 ccggactacg cttcactcgc ctacggtgaa tacggtggca aggctggcat ctggcgcatc
     4381 atggacgttt tcgctcgcaa cggcgttaaa ggtaccatcg ataccaacgg acgcatcgcc
     4441 gaagacttcc ccgacgcatt gcaagaactc catggcgccg gccatgaaat tgtcggtcat
     4501 ggctgggcca atgatttggc gctttcggat gtcgaaaatg ccgccagcga aagagccttg
     4561 atcaagcgca cacttgacgc tcttgcgtcg gtgaccggta aacggccagt cggttgggtg
     4621 agtcccggcc atcgcatcaa tgattacacc tttggaaatt ttgtcgacga aggtattttt
     4681 tggaccggag atttacccgg ccccgacatt ccgcagcatc ggatcgtcaa tggcaagccg
     4741 ctgatcgtca tgccacggct cgattacgca aacgatctct ccagcatctt cacgcccaaa
     4801 aacccgccac gagtttattt cgatagtttc aaagctgcct tcgattgtct gtacgccgag
     4861 ggccaggccg gcgcgccgaa attgatcgac tgtctggttc atgcgcacat cggcgggcgg
     4921 ccgcatctga tcggggtatg ggaagaatgt attcgctacg ccaagggatt tcccgatgtt
     4981 tggttcgcta caaaaggcga gatcgccaaa tattacctcg atacttatgc cgccaaatcc
     5041 taaccggaga aacgaccatg gcgcgcgcga ttgaaaacta cttagctgga ttgaaatctg
     5101 atcagctgtt actgcagttc acctgggacg aatggttggc cattattcca ccttaccctg
     5161 acagagagtt taccagtgcc gatgtcaccg ttcgttatca gcgtaacggg tacgactggg
     5221 acatgcacgg cacgttgtac acgccggcgc aagaaaccga cccgcgacgc gccttggtgt
     5281 tttttcacgg cggcgcgggc agtgaaaaga tcatggactt gacgccggac gggcggccgg
     5341 gtttggcacg agttgccgct gcccagggat tcaaagtcct cgcgctcacc tatccaggtc
     5401 attacccgcc aggaggcgtg tggaaaattc ccattcccga acgcatgccg atctacctct
     5461 tggatcggca gctgaatcag gaagaaattg ctgaccgcaa cttgaaatgc accttcaatg
     5521 tcatactcca gggagccggg gctctgacgg accaacactt ggccggacgt gaaatcatcg
     5581 gcttcggcca ttctaccggc ggtcccatgg ccgcgcacct tacgcgtttc agcaagaaga
     5641 ccaaagtcat cggcctggtt ggatttggca gcggcggccc cgatggttgg cgcaaagagt
     5701 ggcgcgatca gactggcgcg gagaaatatc gggagattcc actggatcat gtgtcgcggc
     5761 gctctcccga ctcgttcaaa aattcgggct acgtcgatcc ggaggattta accccatggg
     5821 gcggcgcaga cgactatatc cgtttggtca gcccactgcg ttcgcaaatg aaaaccagct
     5881 tgtgcgacaa ccagcacaat gcagccattg gaatattgga ggaatacccc aagcgcaccg
     5941 gattgccgcg cgaagaatac ttcgaccact tacaggagcc ggatgcgaat tggctgcgat
     6001 cgattcgcgt cctgctggtc gccggtgaaa acgacaaggg ccactgggtc aacggcggcg
     6061 cacccttgga aaacaaacgc gagatgttca tggggcggaa gtatgccgca accacacgcg
     6121 gcgcccatgt ggtcttcgtt ccacgctatg gccatgttgg ttattccgag ctttacaacg
     6181 aaaaaatcgc ctacctttgg ttatgggcct accagtcggg atacttcgac aattaaagtg
     6241 gcgcaaaaga ggagccgatc atgctgacac gagtaatgct ttgttttctc ctatcaaatc
     6301 ttgttccagt ggaactcacc tttggcgcac aagccaaacc gatgtcaccg gcggagctgg
     6361 cgctttacca aggcaaggat cgcgagcaac tcatcgtcga cggtgccaaa aaagaaggcc
     6421 agatgacctt ctacacttcc aacacctgga tggccgggca tgtctcacaa gaattcggca
     6481 aaaaatatcc gttcatcaag cccaatatct atcgcagcga ctccaaggaa ttgatcaagc
     6541 ggctgactga agaagccggc gcgggacgcc agcttgccga tgtggctgag accggtcccg
     6601 aatacatggc gctcatgcac agcacgggta tgattcagga agtcctgtta cccgaactga
     6661 cgcgctacga cgaggaggcg aaagtcaaag gcaagaaggg cgttctctat tggattagcc
     6721 gcgaactcta catcagcgtc ggctacaaca ccaaactcgt tgctccgggg gatgtgccaa
     6781 aatcaatgaa agatttgctc gaccccaaat ggaaaggcaa aatgacaatc gccggcacga
     6841 cgaccgggac gcaatggatc ggcgccatca tcgaaacttt cgggatggag tttctcgaaa
     6901 agctggcggc gcagaacatc aacgtgcaga atatttccgg tgcggccctc gccgagctcg
     6961 ttgtttcggg cgaagtacca ttgtcaccga caatattcaa ttccaacatc agcaccgcca
     7021 aacagagggg cgcgccagtc gaatggaaac ccatagatcc ggtgatcggc accacgggca
     7081 tgtcagggct agtcgccaaa gcaccgcacg ttcatgccgg tctgctgttt ttggatttcc
     7141 ttcattcgaa agaaggacaa caggccgtca tcaaaggcgg tcttagctcg gcgcgcaacg
     7201 acattgacga cgccgacatg aaattcaaaa aactctacat cgaaagcaag taccctcccg
     7261 acgtgattga aaaaaagttt ggcgaatggg acggattgaa acgcaagcta ttcatcagca
     7321 aacgctagcg agcgcatgag cccgtcggcg catttcggca gattactggc cgtcgcgcta
     7381 ttctgctatt caacggcggc aggaaacgaa gcgcaagccg ctgagaaatc cacaaagtcc
     7441 gcggtcaaaa ttcgcatcag ccagtcggca gtcggcgcgc gttcgacaat tctctggagc
     7501 gcccaagagc tcggtctgtt cgccaagcat ggcattgacg ccgaggtcgt ctatttgcgc
     7561 agtagtccaa tccagatgac cgccctcagc accggcgaag ttcagttcgc cgccagcggc
     7621 ggttcgccgg tactcggcgc tgccgccgcc ggtcaggaca taaaaatagt tgccagtccg
     7681 agtaatcgct tgtcctacga cctagtggtt cggccggaaa taaaagaagc caaggatttg
     7741 cgcggcaagc gcttcggcat caccagcatc ggcggcaccg tctggatggc ggcctatctc
     7801 gccctggagc atctcggact ggatgccaat cgcgatcaga ttcgcatcaa cgccatcggc
     7861 aaccagacga ttctcgccca agcggtggaa tcggcgcaga ttgatgctac gttgctcgat
     7921 ccgtttctca gccggcagac gaaagccaaa gggctgcccg tgctgatcga attgcagcgg
     7981 gcacagattc ccttcaccaa cactgcctgg gccgtcagcg gcagctttct cagagaacgc
     8041 gcggacgcgg ttgaagcggt gctcaaagct atgttcgagg cccaagcctt tatcaccgcc
     8101 ccggcgaacc gcaacgccgt gctgcagatc atggcgcgcc acatgaagct taacgacgca
     8161 gtgcttttgc aagacggtta tcaggatctg ctcgttggct tggagcgtaa accctatgcc
     8221 gcgttggacg gcttacgcaa cattcaacgg atcatgtcga cgatcaatgc caacgccagt
     8281 cgagccaagg ttgaagagat tatcgacaat agaatcgtcc gcaaactcga cgaatccggc
     8341 ttcatcaatg gactttacgc caaacgctag ctgaccaccc taaccgacag tcgacaccgc
     8401 atccaaggag accccatgtt accaaccgta ctcatcaccg gcgcaaacgg ctttatcggc
     8461 actcacttta ccaaattgat ggaatcggcc ggtcataaag tcattcccat cgacgttgtt
     8521 gcgcgcgcac cggatctgtc tctactcggc gtcaaagcga ccagccacct gatgaacgtc
     8581 accgacggcg ccgccttccg tgccctctgt gaaaaggaac gaccaaccca tctgtttcac
     8641 gccgcccacc cgccgcgcga cgagactccc accgtgctcg attattgtta ccgggccatg
     8701 acgaatattc ttgaagcagc aagggactta aaaatgcagc gagtcgttta ctcgagttcg
     8761 gcatcgattt atggacaact ccaaaaagga gcaggcggcc tagtcaagga ggacgacgcg
     8821 ataaccattt atccgaccta tttttaccgt gccgcgaaaa ccgtatccga gtggatgggc
     8881 aatttttaca aagaaaaaca taacgtcgac ttcgtcgcgc tgagatactc ttcggtttat
     8941 ggaccaggcc tttaccgcag catcccgctg gaattgaaga agggaattct cggacagccg
     9001 tgccggccgt ttctcacccg tccgcttgat gacttgatct atgtcgacga cgtcgtcgac
     9061 gccattagcc gagctctttt cgcccaaggt ccgcttagta aagcttacaa tgtcggcctc
     9121 gacaaagctt acgtgagcga cgatctaaag cgcgccatcg aaaaagccct gccgaatctt
     9181 gattttgaaa tcggcaaaca tcctaacgct gccgaagtcg cgccgcaccg gctgcgcagc
     9241 ccgctagaca tatcattggc gcgaaaagag ctgggctggc aaccgaaaat ttatctcgaa
     9301 gacggcatcg ccagattagc ggcctggcta aaaacgcaca aagcagattt aagcttgtga
     9361 aagaatcttc gcctgaagcg cgaaggcgtg ttccaagggc ggtctatggt cgccacggga
     9421 agatattggc tccatcgatc agaaattcaa gaaaaactat ttggacgaca aatattcttc
     9481 cgatgagatg gaagtaaaac tcggccagtg ggaaaactta ttgcgccagc tgtttctgcg
     9541 cagacgctga ataagtccgc caaccgatcg acgagcgatg ccgagcgatt tgattaatcg
     9601 ggttcaagat tcatcgacgt attgacaagg ctaagatgga ttcatagcat cgggacctat
     9661 tctgaaacag agcacaatca tgtcgagaaa attcgctttc gcgctcatcg ctgttttgat
     9721 ctcaacccag ggcttcgccg ccaacgcacc ctcgagcgtc gcgcaaatcg ccctctatca
     9781 gggtggggat cgggaaaaaa ttcttatcga aggcgccaga cgcgaaggtc aggtcacctt
     9841 ctataactcg cacacctggt ttcgtacttt cgttaaagac ttcgaaaaga agtatccgtt
     9901 tatcaaagtc gccgaatggc gcaatgactc gaaaaatatt ttacggcgcg tgaccgatga
     9961 gacgaagtcc ggccggggcc tagccgatgt cattgaaaca acgcccgagg cgatgggcat
    10021 actcaaacgc gacggtatgc tgcaagagta tttttcgcct gaagcccgat tttttcctga
    10081 cgatgtgaaa tccaaaggca aaaacggctt ctactatgtc ggcgaccgcg agacctatat
    10141 tggcctggga ttcaacacca attcgatacc tcaaaccgaa gcacccaaat ctttgcgcga
    10201 cctcctcaat ccgaaatgga aagggaaaat gtccatcgtg accagctcga cgggcacacg
    10261 ttggatcggc aatgctctcg acgcgatggg gcgcgagttc gtcgacaagc ttgctgagca
    10321 ggaggtgcgc ttacaggatt tgtccggcgc ggcattgtcc ggtttagtcg tcaccggtga
    10381 agtgccgttg tcgccgacca ttttcgatgc caatgtcagc gtgcataagc aaaataatgc
    10441 gcccatcgat tggcgtccat tggagccggt cgtcaccaac gttggctatt cgggcatttc
    10501 ggtaaaagct cccaatccgc acgccgccat gctgcttcta gacttcatcc attccaaaga
    10561 aggccagcag ctgatcacga agggcggcct gtggtcgccg cgcgatgata tcggcacggt
    10621 ggagcaacgc tttaaaaaga gttatctcga tgaaaaatat tccatcgagg aattggaagt
    10681 taaatacggc cagtgggaac aattgatccg ccggctgttc ggacaaagaa aatagactct
    10741 gtcgaagaca ggcaaattga ccagggagtc atccatgaaa tctcgaccgc tgccttatcc
    10801 ccatacgata atttttaatg gcaagatccg cactttccag acagaaacat cgacatgcga
    10861 agcgctcgcc tgctcagggt cgcgcatcgt cgccgccgga aagctggaag accttcgccg
    10921 cttcgccggc cccgataccg agatgatcga tctaaaaggg cgcacggcga ttccaggact
    10981 caccgatacc catgtgcacc tttcggaaaa aggcaccgcg gaaatggaac tggtcgactg
    11041 ccgtgacttt tatgtcgatg tgaattcggt ctcggatatc ttgcagcgtt tagccaacgc
    11101 cgctgccaag gcgccgaaag gttcgtggct ggtggctcac ggcagcccga tgcaggattt
    11161 tcgcatcaac gatcatcgtt ttcccgacaa gcatgatctc gaccgcgccg tgccggacca
    11221 tccggtgtcg atctcgttcg gcgcccatgt caccatcggc aatacgttgg cgctcgccgc
    11281 cgctaagatt acccgggaca cagccgatcc cgccggcggc catatcaaac atgatccgca
    11341 gaccggcgaa cccacgggcg aactccatga acgggcgcag ctcattctca gaaaggtcgc
    11401 gccggagttt aattacttgc agctgaagga cggcatcgtc tttgccttga atcaatgcct
    11461 gcaacgcggc gtcaccacgg tgcatgacat cgtccgctac gcggagccgg tgcgcgccta
    11521 ccaggaattg tataaagaag ggcgcatgaa agcgcgcgtg agcatcctgc cacgggttat
    11581 cgaatcgatg atcgagagca aggc
//
LOCUS       DATLCH010000287         3544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_2979576, whole genome shotgun sequence.
ACCESSION   DATLCH010000287 DATLCH010000000
VERSION     DATLCH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2979576"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..933)
                     /locus_tag="VMZ02_08415"
     CDS             complement(82..933)
                     /locus_tag="VMZ02_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR71997.1"
                     /translation="MEQLKPRKSGKVKVDDVNLYYEIYGEGDPLVFVAGTGISCAPWR
                     VSQVPEFAKYYQVVIYDHRGLGRSDKPDIPYSTELFAKDCAGLMDALGINRAHMLGHS
                     MGARVLQWFALNYPERVRSLVLSGPGSGKYDDSLDYPRGVPLDAALEMIEKGYEKYQS
                     DHWGPGFMFSEQFMKEHPDVVKQYQELIVDEVPPLKCYLRHVVARQCHETTHLIHNIK
                     APTLVIVGSKDTHEGGTGDHVASARVLATKIPGAELHLVEGGRHGYLREMPEKGHPPI
                     LNFLKRH"
     gene            1114..2184
                     /locus_tag="VMZ02_08420"
     CDS             1114..2184
                     /locus_tag="VMZ02_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR71998.1"
                     /translation="MKSQFAIFFAIAVVSSAAPSVYSAAAPESAGQILARLNKLPAEQ
                     RHKQLVDKAKTEGEVAFYSSLQAQQIEPFTRVFQKRYPFIKVNPYRVSGNRQVIKIQS
                     ELNGGNHLFDVTNGSAEQASAIKKIGAIDPYFSPQREYYNPPNRDKDGYFTSLYVIPI
                     VLGYNTNMVKRQDAPKSYEDLLQPKWKGNMFLDDEAYEWSAVLQKHMGRDKALQYMRA
                     LAKQDLRLMRGRTAQSQLLAAGERSIAIVLSGHTVLDMKARGAPIDQVILDPYFAQAN
                     KLMLARHAPHPHAAALFYDWSLSEEGQSIITSFGRVIARKGVKQRFPELVEKESFLVD
                     VDFIGPIMDQTGKEFSQIFLGR"
     gene            complement(2209..3162)
                     /locus_tag="VMZ02_08425"
     CDS             complement(2209..3162)
                     /locus_tag="VMZ02_08425"
                     /inference="COORDINATES: protein motif:HMM:NF017475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HUR71999.1"
                     /translation="MAGSSLFALLDDIASILDDVAVMSKVAAKKTAGVLGDDLALNAH
                     QIAGVHTDRELPVVLAVAKGSLKNKAILVPAALAISALIPWAITPLLMIGGAYLCYEG
                     CEKIAHKFLAHSSDDAHHKDHLQALVNPAVDLVALEKDKIQGAIRTDFILSAEIVVIA
                     LGTVANAAFGKQVAVLCAIAILMTVGVYGFVAGIVKLDDAGLHLCQKNGNNTLRKIQR
                     GFGALLLHLAPYLMKSLSIVGTIAMFMVGGGILAHGIPGAHQWVEHVAHGAGGIPAIG
                     RILEPVLPIVLDIAIGMIAGMVVLAGMMVAEKIFRRFKRAR"
     gene            complement(3271..>3544)
                     /locus_tag="VMZ02_08430"
     CDS             complement(3271..>3544)
                     /locus_tag="VMZ02_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72000.1"
                     /translation="LTFHVASIWGIEGRGLLRPGFAADVTVFDPRTIKACEPEWAEDY
                     PAATKRLIQRSEGVHYTVVNGKVIFDQGRLSGEMAGNVLRSAAHHG"
BASE COUNT          818 a         1045 c          932 g          749 t
ORIGIN      
        1 agacgcctgt tagcacagag gacgaccgcc ggtcgcccct atacttcatt ttgcttcgtg
       61 ccttcttagt gagttccacg attaatgtct tttcagaaaa ttcaggatcg gcggatggcc
      121 cttctcgggc atttcacgca aatagccgtg ccggccgccc tcgacgagat gaagttcggc
      181 gccgggaatc ttcgtcgcca gcactcgtgc cgaagcgaca tgatcgccgg tgccgccttc
      241 gtgagtgtct ttactgccga cgatcaccaa agtcggcgcc ttgatgttgt gaatcaggtg
      301 agtcgtctca tggcattggc gcgcgaccac gtggcgcaga tagcatttta acggcggcac
      361 ttcgtcgacg atcagctcct gatactgctt aaccacatcg gggtgctctt tcatgaactg
      421 ttcgctgaac ataaaacccg gcccccagtg gtcgctctga tatttttcat aacccttttc
      481 gatcatctcc agcgccgcgt cgagcggcac gccgcgcgga tagtcgagag agtcgtcgta
      541 ttttcccgag cccgggccgg agagcaccaa gctgcgcact ctttccggat agttaagcgc
      601 aaaccactgc aatactcgcg cgcccatgga atggcccagc atatgcgccc ggttgatgcc
      661 cagagcatcc atcaaaccag cgcaatcctt agcaaatagt tcggtggaat agggtatgtc
      721 cggcttgtcg ctgcgcccca ggccgcgatg atcgtaaatt acgacttggt aatatttagc
      781 gaactccggt acctgcgaca cgcgccaagg tgcgcagctg atgccagtgc cggcgacaaa
      841 caccaatgga tcgccttcgc cgtaaatctc atagtaaaga ttgacgtcgt caactttgac
      901 cttgccgctc ttgcgtggtt tcaattgctc catctgatct ccaagttacc gatatgtagt
      961 cgcgctgacg ctatcgaatc agtaatttag agtcaacatg agtccaggat tgaaagcgca
     1021 acaataacct ctacttgaca gttccacggc gcgctgggat aaggatggcg ctgagtccaa
     1081 tgcgcatgaa tggaaatcaa cggaaggtcg gccatgaaaa gtcaatttgc aatcttcttc
     1141 gccatcgccg tcgtgagcag cgcagcaccg tcggtttatt ccgctgcggc gccggaatca
     1201 gccggtcaaa tcctcgcgcg attgaacaag ttgccggcgg aacagcgaca caagcagctt
     1261 gtcgacaagg ccaagaccga aggcgaagtc gctttctata gctcgcttca ggcgcaacag
     1321 atcgaaccct tcacccgcgt gtttcaaaag cgctatccat tcatcaaagt gaatccctac
     1381 cgtgtctccg gcaaccgcca ggtgatcaag atccagtccg agttaaacgg cggcaatcat
     1441 ctcttcgatg tcaccaacgg atctgccgaa caggcctcgg cgatcaagaa gatcggcgcc
     1501 atcgatccgt atttctcgcc gcagcgcgaa tattacaatc caccgaatcg cgacaaagac
     1561 ggttacttca cttcgctcta cgtcatcccc atcgtgctcg gatacaacac caacatggtc
     1621 aagcgccagg acgcacccaa gagctacgaa gacctgctcc agcccaaatg gaaaggcaac
     1681 atgtttctcg atgacgaggc gtacgagtgg tccgctgtgc tgcaaaaaca tatggggcgg
     1741 gacaaagcgc tgcagtatat gcgcgcgcta gccaagcagg atttgagact catgcgcggg
     1801 cgcaccgccc agagtcagct cctggccgcg ggcgagcggt ccattgccat cgtgctgtca
     1861 ggacataccg tcctcgacat gaaggcgcgc ggcgcgccga tcgatcaagt aatcctcgac
     1921 ccttacttcg cccaggccaa taagctcatg ctggcgcgcc atgcgcccca ccctcacgcg
     1981 gccgcgcttt tttatgattg gtcgctgtcg gaagaaggtc aatcgatcat caccagcttt
     2041 ggccgggtga tcgcgcgcaa aggagtcaag caaagatttc ccgagttggt ggaaaaagaa
     2101 tcgtttctcg tcgatgtcga tttcatcgga ccgattatgg atcagaccgg caaagagttc
     2161 agccagattt ttctcggccg gtaacgatcg gaccgaacat cttcaagttc agcgcgcccg
     2221 tttgaacctg cgaaaaattt tctccgcgac catcatccca gccaaaacta ccataccggc
     2281 aatcatcccg atcgcaatat cgagcacgat tggcaaaacc ggttcaagta ttcgaccaat
     2341 cgcaggaatc ccgccggcac cgtgggcgac atgttcaacc cactgatgcg cgccgggaat
     2401 tccgtgcgcg agaatgccgc cgccgaccat gaacatagca atggtcccga caatcgagag
     2461 acttttcatc aggtaaggcg ccagatgcag gagaagtgcg ccaaaaccgc gttggatttt
     2521 gcgcaaagta ttgttcccgt ttttctggca caagtggagg ccggcgtcgt cgagtttaac
     2581 gatgccggca acgaatccat aaaccccgac ggtcatgaga attgctatgg cacagagcac
     2641 cgcaacctgt ttgccgaaag cggcgttcgc aaccgtcccc aatgcgatca caacgatttc
     2701 ggctgacaaa atgaaatccg tacgtatggc gccctgaatt ttgtctttct ccagcgcgac
     2761 cagatcgacg gctgggttaa ccagagcctg cagatggtcc ttgtgatggg cgtcgtccga
     2821 cgagtgagcg agaaacttat gagcgatctt ttcgcagccc tcgtaacaca gatacgcccc
     2881 gccgatcatt aacagcggtg taatcgccca cggaatcaat gcgctaatcg ccaacgccgc
     2941 gggcaccagg atcgctttgt tcttgagcga gcctttggcg acggccaaaa ccaccggtaa
     3001 ttcccggtcg gtgtgcacgc cggcaatctg atgggcattg agcgccagat cgtcgcccaa
     3061 taccccggcg gttttcttcg cggcgacttt gctcattacg gcaacgtcgt caagaatcga
     3121 cgcgatatcg tcaagcaggg cgaataaact acttccggcc acggatcact cccgtgctga
     3181 tcactgtcga gataaaaaag agggcgggtt tgaaacccgc ccttgcgact ggaatttccc
     3241 gctgtacgtt gttgggtgaa tcttccgatt tcacccgtgg tgcgccgcac tgcgcagcac
     3301 attgccggcc atctcgccgc tcaaccggcc ttggtcaaaa atcaccttgc cgttgacgac
     3361 ggtgtaatgc acgccctcgg agcgctggat caagcgcttg gtggcggcgg gataatcttc
     3421 ggcccattcg ggttcacagg ccttgatcgt cctgggatca aaaacggtca cgtcggcggc
     3481 gaacccgggg cgcaatagtc cgcggccttc gatgccccaa atggacgcga catgaaacgt
     3541 caac
//
LOCUS       DATLCH010000288         9357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_9687728, whole genome shotgun sequence.
ACCESSION   DATLCH010000288 DATLCH010000000
VERSION     DATLCH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9687728"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VMZ02_08435"
     CDS             <1..253
                     /locus_tag="VMZ02_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72001.1"
                     /translation="AMLTSKSVTVKFYEGSTPTCVEYVQVTAQGTYNFFFYQLMRIVG
                     MSAPNTTLISRTTQMRYTYQPFDNTAACTATSVNQSYSS"
     gene            270..1283
                     /locus_tag="VMZ02_08440"
     CDS             270..1283
                     /locus_tag="VMZ02_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HUR72002.1"
                     /translation="MKTLNNQKGTVLIFATLMIVVLLVLVGMGLDAGHLAFIRSQGQP
                     AVDAAALAAASAIPTGNLSTVQARAASINPGGSNPGIGNNYLNSPGNAIGNSNLTLVQ
                     YNSSNGAVTTTGVTIANANAVRVGLEKDNPYGGSPGAAMKSPLFLTPLMKLLGASTSA
                     TTDVNVSAIAAIKAIPGMPIAIDQTRCGQSGNIDLEFNPQGDSGWTTYSLKANTTNIK
                     ALFETLPSCGGSPAVEVGFCTNLQNGTIGNLFNDTIPDLFKAHPTDCYLLPVVGDIKN
                     HNFNQCAPITDWAKFCPNSNLSKAVDGHKLNGTVTCGQSPYTSRDTRCYVPFLVRDVK
                     SGM"
     gene            complement(1289..2473)
                     /locus_tag="VMZ02_08445"
     CDS             complement(1289..2473)
                     /locus_tag="VMZ02_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72003.1"
                     /translation="MASNVTHQFISADDHIDLRWLPKDLWHQRLPARLRERGPRVIEN
                     DKGAFWSWEDQTFSPHGYYTAAQGSGAMWAIERGGVMRPGELRPTTAALRLSDMDRDG
                     AEVSIMYGPTDPMVITDPELRRLCYQAYNDWLSEFCAAQPERLIGVPQLSMEDPLAAR
                     DELARLAQRGGLRHVNILASRAHPPVYDDAWEPFWALAEEVGIPVGFHLAVMVKKTRL
                     DDLQRGATNLVVTVAAQYAQEPPGIQLLEPMTGLIFNGVLDRHPRVQIVMAEAGLAWI
                     PNMIQGLDIWYQRSRDGRRLTGERPVILPKLLPSEYFHRQIWISFVDDPLGVKMVGNV
                     LDADKVMFGSDYPHPASTWPNSQSVIAAQMECLPEAIVQKITCDNARALFGIPRQDLR
                     PS"
     gene            complement(2463..3467)
                     /locus_tag="VMZ02_08450"
     CDS             complement(2463..3467)
                     /locus_tag="VMZ02_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72004.1"
                     /translation="MRQITRGFLRLLLATGLVLRLSVVWAGEPGLKLVDVSLPAVSLL
                     QSPLFAAMESGAFKKYGMEVRYIVTGARSIQALIGGSVNFAQGVSSRTVPSAVLAGFD
                     AVLIANFSDKFLFTMHSAPEINSLQDLRGKIVGVSGLGGSTDFATRVALREAGLVPDK
                     DVMIRGVGGVPETVAALRAKLVHAGTLSPPSSFVAQKAGFKMLFDMTSLGVDYVSSGL
                     GVKKTWLVANRLEAKQFVMAMIEGAKALTTDEEFAMRVLTKHTRIADREVLKQSYHYI
                     KPYFLKAPYPSTRAIKDTLDALAKDLPKAKDADPRDFVDLSIVKEIEASGFIAGVYGK
                     "
     gene            complement(3549..4130)
                     /locus_tag="VMZ02_08455"
     CDS             complement(3549..4130)
                     /locus_tag="VMZ02_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72005.1"
                     /translation="MRELIVIGALLMGVYLWTGREISRPAGVLVEEEPVQRQVYTASI
                     REKNGYRIELLATFDIRGRVIGVATYRFDRAAQIAPVDLALGWGAMSDSAVLRHISFS
                     QGGRFYSWWAKDYPVPRTIIETHSANMHMIPANDGIERQLKSLRPGNLVHIKGFLVEV
                     SNKEGLHWKSSLTRSDTGAGACELLWVESLASW"
     gene            4709..5524
                     /locus_tag="VMZ02_08460"
     CDS             4709..5524
                     /locus_tag="VMZ02_08460"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HUR72006.1"
                     /translation="MAKYALCIGINDYPGTDNDLSGCVNDANDWATALSKRGFATTKM
                     LDKQATGKNMRAAITAAIAKANAGDLVVIQYSGHGSYVPDDDSEEPDGKDECLCPYDI
                     NSKGPITDDELFEIYSAKKTGVKLLVISDSCHSGSNARFAPITTPPTIKGKNAPQRKV
                     RFLPPAVFLSKSQLAKLGSSRAMRRSNPPGRHAALLMAGCQDSEYSYDASFEGRPNGA
                     YSFVALRELVKLPANASYQRWYDAIRKVLPSQQYPQTPNLYGTKSMKGWRVFV"
     gene            5661..6500
                     /locus_tag="VMZ02_08465"
     CDS             5661..6500
                     /locus_tag="VMZ02_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HUR72007.1"
                     /translation="MRKSTLRLHGRRRASLFLQEGSTGPAVAKLQAKLKKIGFNPGRV
                     DGDFGPATKAALIGFQKSAGLLADGIAGPRTQTALGLTANDNLPSVIPGITVTMVSKI
                     FPVTPVDNIRNNLPFVLQGLVERALGDKPMVLMALATIRAETECFQRINEGLSKYNTS
                     PGGHPYDLYDFRSDLGNRAKGDGARYCGRGYIQLTGRFNFQKYGTALGMGNKLALEPE
                     LASDPTVAGKLLAAFLKDKERAVKESLILGDLCMARRLVNGGSHGLDRFIDAFRIGDA
                     LIA"
     gene            6552..8639
                     /locus_tag="VMZ02_08470"
     CDS             6552..8639
                     /locus_tag="VMZ02_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HUR72008.1"
                     /translation="MPKRTLEIGGNKVTLDARPDRLDIRDLPYRPSVVSLPPIYPAEK
                     DVAALLSNYIAAGLILDQKSEGACTGFGLAGVINFLLWRRSKHRMKQKDRVSPRMLFH
                     LAKFYDEWPGEDYEGSSCRGALKGWHRHGVCRNSLWPFKDKQFVRPHEGWDIDALCRP
                     LGVYYRVERDSVVDLQAAIFDTGAVYVSANVHAGWEVKALKGIIRHKSLPIIEAGAEG
                     YGGHAFALVGYNDTGFIVQNSWGEKWGGRGFAVLPYEDWVVNGTDAWVVGLGAPVQRL
                     QQRQYFVKHSDGIAGHIARSGMIGPAQAKLKDSTDLWSEQNAYWHSVVTGNDGQIINR
                     LPQVQDERDNVAWTTYEQPLKWFKEHSNSAAWRIALYAHGGLNAEASSIDRIRTLGPY
                     FSENGVYPLFTTWKSGWQEIFANMLADGADKLFGGQIVPSRGLGDLLQEASDRALEVF
                     VRTVLGKSMWSEMKENVTQSSEQGRGIDEIAVQLARLQKASANRLEIHLIGHSAGSFV
                     CGRLLTELRQRKLKAHSCTLFAPACDLEFALEHFKPAIEENHLPRSKFRVYVLSDSSE
                     LNDTVGPYGKSLLYLVSRALERRHKTPLLGLVNAFDGERATDEYWHSDTIRSWVKPWQ
                     HFFWQGKPPRGFADQGSAAPGKNLQILGTSIGSNSVKNCHGCFDNNVEVIGETIEAIT
                     GGELRRPIINLDY"
     gene            9027..9191
                     /locus_tag="VMZ02_08475"
     CDS             9027..9191
                     /locus_tag="VMZ02_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72009.1"
                     /translation="MRLIRHSFIITTLYGVGVVEAFYLPRYGLEWFLGAIGLTVFSAG
                     LWMRKNWDRS"
BASE COUNT         2158 a         2724 c         2487 g         1988 t
ORIGIN      
        1 cgctatgctc acgagcaaga gtgtcacggt caaattctac gagggcagca cgccgacctg
       61 tgtcgaatac gtgcaggtca ccgcacaggg aacctataat tttttctttt accaactcat
      121 gcgaatagtc ggaatgagcg cacccaatac gacactcata agccgcacca cgcagatgcg
      181 ttatacctac cagccttttg acaacaccgc ggcttgtaca gcgaccagcg tcaaccaaag
      241 ctattcgagt tagacaaccg gagtgagcca tgaagacttt gaataatcag aagggcacag
      301 tactcatttt cgcgacttta atgatcgtcg tgttgctcgt tctagtcggc atgggtctcg
      361 atgccggcca tcttgcgttc atccgatcgc aaggacagcc cgccgtcgac gccgccgcgc
      421 tcgccgccgc atcggcaatt ccgacgggta atttgtcaac tgttcaagca cgcgccgcgt
      481 cgatcaatcc cggtggcagc aatcccggca tcggcaataa ctatttgaac agcccgggta
      541 atgccatcgg caatagcaac ttgaccttgg tgcaatacaa ttcaagtaat ggtgccgtca
      601 cgaccaccgg cgtaaccatt gccaatgcca acgctgtgcg cgtcggtctg gaaaaagaca
      661 atccttatgg cggcagtccc ggcgcggcga tgaaatcgcc gttgtttctc acaccattga
      721 tgaaattgct cggcgccagt acctcagcga cgaccgacgt caacgtcagc gcaattgccg
      781 cgataaaagc aatcccggga atgccgatcg ccatcgatca aacacgatgt ggtcaatccg
      841 gaaatattga cttggaattc aatccccagg gtgattcagg atggaccacc tattcgctca
      901 aggcgaacac gactaatata aaagctctat tcgagaccct gccgagttgc ggcggctcgc
      961 cggccgtgga agtggggttt tgcaccaatt tgcagaacgg cacgattggc aacctcttca
     1021 acgatacgat acccgatctt ttcaaggccc acccgacgga ctgttatcta ctgccggtgg
     1081 tcggcgatat taagaatcat aacttcaacc aatgcgcacc gatcaccgat tgggcgaaat
     1141 tctgtccgaa ttcgaacctc agcaaagccg tcgacggcca caagctcaac ggcactgtga
     1201 cttgcggtca gagtccctat acctcgcgcg acaccagatg ttatgtcccg ttcctcgtgc
     1261 gagacgtaaa atcaggaatg tgattttctt acgacggtcg caaatcctgt cgaggaattc
     1321 caaacagcgc ccgcgcatta tcgcaggtaa ttttttgaac aatcgcttcg ggcaaacatt
     1381 ccatctgcgc ggcgatcact gattgcgagt tgggccaggt gctggcggga tggggatagt
     1441 cggaaccgaa catcaccttg tcggcgtcga gtacattgcc gaccattttc acccccaggg
     1501 gatcgtcaac gaagctgatc cagatctgcc ggtgaaaata ttcgctcggc aggagcttcg
     1561 gtaatattac cggcctttcg ccggtcaagc gcctgccgtc gcgactgcgc tggtaccaaa
     1621 tatcgagacc ctgaatcatg tttggtatcc atgcgagccc ggcttctgcc atgacgattt
     1681 gcaccctcgg atgacgatcg agaacgccat tgaatatcag tccagtcatt ggttcgagca
     1741 actggatgcc gggcggctct tgcgcgtatt gcgcggcgac cgtgacgact aaattcgtcg
     1801 cgccgcgctg gagatcatcg agccgggtct tcttgaccat caccgcgaga tgaaagccga
     1861 ccggaatacc gacctcttcg gccagtgccc aaaacggctc ccaggcatcg tcgtacacgg
     1921 gcggatgcgc gcgcgacgcg agaatattga cgtgacgcag gccgccgcgt tgagccagac
     1981 gcgccagttc gtcgcgcgcc gccaacggat cctccatcga caactgcggc acgccgatca
     2041 accgctccgg ctgcgccgcg cagaactcgc tcaaccaatc gttgtaggct tgatagcaca
     2101 atcggcgcag ttccgggtcg gtgatcacca tcggatcggt ggggccgtac atgatcgaga
     2161 cctcggcgcc gtcgcggtcc atgtcgctca agcgcaacgc cgcggtcgtc gggcgcaatt
     2221 cgcccgggcg catgacgccg ccgcgctcga tcgcccacat ggcgccgctt ccttgcgctg
     2281 cggtgtagta gccatgggga ctaaacgtct ggtcttccca actccaaaac gcgcctttgt
     2341 cattttcgat cacccgcggc ccgcgctcgc gcaggcgcgc gggcaagcgt tggtgccata
     2401 aatccttcgg caaccagcgc agatcgatgt gatcgtcggc agaaataaat tggtgcgtca
     2461 cgttacttgc catatacgcc ggcgatgaaa ccgctagctt cgatttcctt gacgatcgac
     2521 agatcgacaa aatcgcgcgg atcggcgtcc tttgctttgg gcaagtcctt ggctaacgca
     2581 tcgagggtat ctttgatcgc ccgcgtcgat ggataaggcg ccttgagaaa atacggtttg
     2641 atatagtgat agctctgctt caaaacttcg cgatcggcaa ttctcgtgtg tttagttaac
     2701 acccgcatgg caaattcctc gtcggtcgtg agagcctttg ccccctcgat catcgccatg
     2761 acgaactgtt tcgcttccag acgattggcg acaagccagg tcttcttgac cccgagaccg
     2821 gaggaaacgt agtcgacgcc caagctggtc atgtcgaaaa gcatcttgaa ccccgccttc
     2881 tgcgcgacaa atgacgacgg cggcgacaag gtgccggcat gtactagttt ggcccgcagc
     2941 gcggccacag tctccggcac tccgccgaca ccgcgaatca tgacatcctt gtcaggaacc
     3001 aggccggctt cgcgtagcgc cacgcgggta gcgaaatcgg tgctgccgcc gagccccgaa
     3061 acaccgacaa tcttaccccg gagatcttgc agcgagttga tttccggcgc gctatgcatc
     3121 gtgaacagaa acttatcact gaagttggcg atcaacaccg catcaaatcc ggccagcacc
     3181 gccgacggca cggttcgact tgaaactccc tgtgcgaaat ttaccgaacc cccgatcaat
     3241 gcttggatac tccgtgctcc tgtgacgatg tagcgcacct ccatgccgta cttcttgaac
     3301 gcaccgcttt ccatcgcggc aaacagcggc gactgcagca acgacactgc cggcagcgag
     3361 acgtcaacga gcttcagtcc cggctccccc gcccagacaa ccgatagccg caacaccaat
     3421 ccggtcgcta atagcagtcg caaaaacccg cgcgtgatct gtcgcatgct ctctcccgta
     3481 tcttggatga ctacgggcgg tttactccaa gcacattgga atgtcgagaa gtttggccgc
     3541 cggaagcctt accatgacgc caacgactcg acccaaagca actcgcaggc accagcaccg
     3601 gtgtcgctgc gcgtcaaaga acttttccag tgaagacctt ctttgttgga gacctccacc
     3661 aagaatcctt tgatgtgaac tagattgccg ggacgcaggg actttaattg ccgttcgata
     3721 ccatcattgg cgggaatcat gtgcatgttt gcgctgtgag tttcgatgat agttcgcggc
     3781 accggataat ccttggccca ccaactgtaa aagcgcccgc cttgcgagaa gcttatgtgc
     3841 ctcaagacag cgctatcgga catcgctccc caacccaaag caaggtctac cggcgcgatt
     3901 tgtgcggcgc gatcgaagcg atatgttgcg acaccgatca cccgcccgcg aatatcgaag
     3961 gtcgcgagaa gctcgatccg atagccattt ttctcgcgaa tcgatgcggt gtagacctgc
     4021 cgctgcacgg gttcctcctc cacgagcacc ccggcaggcc ggctaatctc gcgaccggtc
     4081 cagagatata cgcccatgag cagggcaccg atgacgataa gttctctcat gcaaatctgt
     4141 ccacgggcta gcttagccga actctggcgg ctgaaaaaga tttaagtcga acgacgcaca
     4201 ctttgcttgc attatcgcca gcgcttaagt atccaaggcg tcgatcgtct catgacgcga
     4261 attgcgacag acgtcgttaa agaatcatgt gatatcaacc acgggaggac cgatgaacgg
     4321 ttcaatgtgc cggacgcttt tcttgttggc gacaatctca ggcgccccgt gtgcccgtgg
     4381 ccgctgtgaa ataaaataac cgcgcgcgca gcgccatgca cctgaactgg ccagtggcgt
     4441 aaaagagcgt cgccccatcg gcgctcgcga accaattttc tcttgcgggg cgcgcacgag
     4501 ccccctgtcg aacctgctga ctcgaaaaat cgatggcttg attcatttgc acgatttgac
     4561 tcgttctttc atcgcttgaa cggtgacgtc actttgttcg tcgtcaggta gtcgcaatga
     4621 atttattttt tcgatcgaaa tctcataacc tttttcgcaa aatttgcgtt acaatgcggc
     4681 ctcaaataaa cctaaggaga tttttttcat ggcaaagtat gccctgtgta tcggtatcaa
     4741 tgattatccc ggcaccgaca acgacctttc cggttgtgtc aacgacgcca atgattgggc
     4801 cacagctctc tccaagcgcg gctttgcgac aaccaagatg ctcgacaagc aggccaccgg
     4861 caaaaatatg cgcgccgcga ttacggcggc gattgccaaa gccaatgccg gcgatttggt
     4921 ggtgatccag tattcaggac atggatccta tgtgccggat gacgacagcg aggagccgga
     4981 tggtaaagac gaatgcctgt gcccatacga catcaattct aaaggaccga tcaccgacga
     5041 cgaactcttt gagatttaca gcgcaaaaaa aaccggtgta aagctgttgg tcatttccga
     5101 ctcttgccac tccggcagca atgccagatt cgccccgatc acgacaccgc caacgatcaa
     5161 aggtaaaaat gcgccacagc gcaaggtgcg cttcctgccc cccgcggtgt tcctgtcgaa
     5221 atcgcaatta gcaaagctcg gctcgagccg cgcgatgcgc cgctccaacc cgccgggacg
     5281 acacgccgca ctgctaatgg cgggatgtca agacagcgaa tatagctatg acgcgtcctt
     5341 tgaaggccgt cccaatggcg cctacagctt cgtcgcgttg cgcgaattgg tcaagctgcc
     5401 ggccaacgcc agctatcaac gctggtatga cgcgatccgc aaagttctgc cgtcgcaaca
     5461 gtatccccaa acgccgaact tatacggcac taagagcatg aaaggctgga gggttttcgt
     5521 ctgacagaac agcaagtttt ctgtttgtca ggcagggaca ctcactgata cagccctgag
     5581 tcttccgtgc tgcatcgcta tttccgctac gaacgccaca accatcgatt cgctcgatat
     5641 cagttcggca aggagaaccc gtgagaaaat ccactctacg tttgcatggc cggcgcagag
     5701 cttcgctatt cttgcaggaa ggttccacgg gcccggccgt agccaaactg caagcgaaac
     5761 taaaaaaaat cggctttaac cctggccgcg tcgatggtga ttttggccct gcaaccaaag
     5821 cggcgttgat cggctttcaa aaaagcgccg gcctcctcgc cgatggcatt gccgggccgc
     5881 gcacgcagac agcactgggg ctaaccgcga acgataactt accctcggtc attcccggca
     5941 tcaccgtcac tatggtgtcg aaaatatttc cggtcacgcc ggtggacaac atccgcaaca
     6001 acctgccgtt tgtattgcaa gggttggtcg aacgggcgtt gggcgataaa ccgatggttc
     6061 tcatggcact ggcgacgatc cgtgccgaga ctgaatgttt tcaacggatc aacgagggac
     6121 tttccaaata caatacttct cccggcggcc acccgtacga tctctatgac ttccgctccg
     6181 acctgggaaa tcgagcaaag ggggacggcg ccagatattg tggtcgcggc tatatccaac
     6241 tcaccggtcg tttcaatttt cagaaatacg gcacggctct gggcatgggc aacaaacttg
     6301 cgcttgaacc ggagctcgcc agcgatccga ccgtcgcggg caaattgctg gcggcatttc
     6361 tcaaagacaa agagcgcgcc gtcaaagaat ccctgatcct tggcgatttg tgcatggcgc
     6421 gtcggctcgt caatggcggc agtcatggcc tggatcgctt catcgatgcg ttccgtatcg
     6481 gcgatgcatt gatcgcgtga cgtagtctaa aaaatctcag acagtcttca tttgtaccga
     6541 aggacaattt catgcctaaa cgaacattgg agatcggcgg caacaaagta actctcgatg
     6601 cccgcccgga ccgcctggac atccgagatc taccgtatcg gccatcggtc gtctccctgc
     6661 cgccgattta ccctgccgaa aaagacgttg ctgccctact gtcgaattac atcgccgccg
     6721 gattgattct cgaccagaaa tcagaggggg cctgtaccgg attcggcttg gcgggagtga
     6781 tcaatttttt gctctggcgc cgatcgaagc acaggatgaa acaaaaggat cgagtcagtc
     6841 cgcgcatgct gttccatctg gcgaagtttt acgacgaatg gccgggcgaa gattacgaag
     6901 gttcgagctg tcgcggcgcc ctcaaggggt ggcaccgcca tggcgtctgc cgcaactcac
     6961 tctggccctt taaggacaag caattcgtcc gaccacacga aggttgggac atcgatgcgc
     7021 tatgccgtcc tctcggtgtc tactaccgtg tcgaacgcga ttcagtggtc gatctgcaag
     7081 cggctatttt cgacacgggc gccgtttacg tctcggcaaa cgtgcatgcc gggtgggaag
     7141 tcaaagcgct caaagggatc atccgacaca aatccctgcc gataatcgag gccggcgccg
     7201 aaggttatgg cggccacgcg ttcgccctgg tgggatacaa cgacaccggt ttcatcgtgc
     7261 aaaactcttg gggcgaaaaa tggggggggc gcgggttcgc cgttttaccg tacgaagact
     7321 gggtagttaa cggcaccgac gcttgggttg tcggcctcgg cgcgcctgtg caaagactgc
     7381 agcagcgcca atacttcgtc aaacacagtg acgggatcgc tggtcatatc gcccgctccg
     7441 gtatgatcgg accggctcag gccaagttaa aagactccac cgacctttgg tccgagcaaa
     7501 acgcctattg gcacagtgta gtcaccggca acgacggtca gatcatcaat cgcctacctc
     7561 aggttcaaga cgaacgggac aacgtcgcct ggacaacata cgaacagcca ctcaagtggt
     7621 tcaaagaaca ctccaacagc gctgcctggc gcatcgcact ctacgctcat ggcggcttga
     7681 acgccgaagc atcgtccatt gatcgcattc gtaccctagg tccctacttt agcgagaatg
     7741 gcgtatatcc cctgttcacg acctggaaaa gcggctggca agagattttc gctaacatgc
     7801 tcgccgacgg tgccgataaa ctgttcggcg gccaaattgt accgagccgc ggcctcggcg
     7861 atttattgca ggaagcatcg gaccgcgcat tggaggtgtt tgtgcgcact gttttaggca
     7921 agagcatgtg gtcggagatg aaagagaacg tcacacagtc gagtgaacag ggtcgcggca
     7981 tcgacgagat cgccgtgcag ctggcgcggc tgcaaaaagc ctcggccaat cgtctggaga
     8041 ttcatttgat cggccactcg gcaggatctt tcgtctgcgg ccgactgttg acggaattgc
     8101 ggcagagaaa attgaaggcg cacagttgca cgctgttcgc gccagcttgc gacttggaat
     8161 ttgccctgga gcatttcaag cccgctatcg aagagaacca cctgccccga tcaaaatttc
     8221 gcgtttacgt actctcggat agcagcgaac tcaacgatac ggttggacct tacgggaaat
     8281 cattgctcta tctggtcagc cgcgccctgg agcgtcggca caaaacgccg ctcctcggac
     8341 tggtcaatgc ctttgatggc gagcgcgcca ccgacgaata ttggcatagc gatacgattc
     8401 gaagctgggt caaaccctgg cagcatttct tctggcaagg aaaaccgccg cgcggcttcg
     8461 ccgaccaggg tagcgccgct cccggcaaaa acttgcaaat acttggcacg tccatcggat
     8521 ctaatagcgt gaaaaactgt cacggctgct ttgataacaa cgtcgaagtt attggcgaaa
     8581 ccatcgaggc aatcaccggc ggcgagctgc gtcggccgat tatcaatttg gactactgag
     8641 cgcaagtatc gcgcatcgcg gtatgatatt tacccgtcat cgaggctcgc tcgatcgggt
     8701 cgaaaattca gtattactgt cgggaaaggt ccgcatttcc gcagatcagc catcggctgt
     8761 cttgcgctca gctactcgca taatgcgcga ttgaaaatgg agaaaaattc gtttcccgac
     8821 caataagatc gatttcatcc ttgcttcacc aagcgtcggg aaatgcctgc gcatgcgctc
     8881 acgcaatcgt aggtaatcac ggtcacactc ggtttcccgg cgaaaaattt ccggtcggat
     8941 catcatcttt tattctacga aaacccggat cgctgacgct tttcctaagg tgtaaagatg
     9001 gtacggatcg tgcgctgcca atggtcatga gattaatccg gcatagcttc atcatcacga
     9061 ccctctacgg tgtaggcgtt gtggaggcct tttatctgcc gcgctacggc ctcgagtggt
     9121 ttctcggcgc catcggcctg acggtgttca gcgcgggcct gtggatgcgc aagaactggg
     9181 atcgctcata gacccgtcgc ccactactac agaattgatc gcgcgcggcc gggtcagcgg
     9241 cacttgagtt gaaaaatcgc actacgctga caactgccgc gacgctgcga ttccaagaca
     9301 ccttattagc gtaagcagcg atatcgaagg gtcatcaaag gactgcagcg attcttt
//
LOCUS       DATLCH010000289         6262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_5954653, whole genome shotgun sequence.
ACCESSION   DATLCH010000289 DATLCH010000000
VERSION     DATLCH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5954653"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1256)
                     /locus_tag="VMZ02_08480"
     CDS             complement(189..1256)
                     /locus_tag="VMZ02_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72010.1"
                     /translation="MAEFIIDADGHIMEDHKDIFAHIKGNFAEMNWHSTWPMFDADGW
                     QRGLARKGKREDPDAERWIKFQNEHGIDMAVLYPTSALALGMIQLPAWASALAQGYND
                     WLYDRFMSQSPRLKGVALLAPQDPKAAAAELRRCVKDLGFVAGLLPSATNNRTPLYGS
                     PVFHEIYDEAQRLDVPLTIHGGVSQNLGLDRVASFLEAHMLEHPVGLFLHITNMMFQG
                     IFQEFPKLRVAYLEAGAGWVPFMMDRMEEDYEKFAARLAPNLKSPPSKFFKSGNIFVT
                     MEVEERTAPYLADLIRDDVIMWASDFPHERERDQFGGDLPHLKARKDLSDEFKQKMLY
                     DNPVRFYRFSEGDIAAVKKAK"
     gene            complement(1410..2417)
                     /locus_tag="VMZ02_08485"
     CDS             complement(1410..2417)
                     /locus_tag="VMZ02_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72011.1"
                     /translation="MRKLSALLFVCLGLVQGHSAYAQSAQAVAAAKKEGGKVIVYTSM
                     ETFTADALKAAFEKRTGLQMDYWRGGSTEVLDRVLSEHRVGKPGFDVVAITGDHMRLM
                     ANEGAIGKYDSPSLKAFAKEAIDPVLGARYRNIMYGVIYNKAQLKAGEYPKTLEDIVK
                     PEYRGKLVMPHPTGHTLTTQWLASLDKIMPKQRVEKFVRDLAAAKPVFAESIVPAADK
                     VGTGEVPIGITFARFVLTYNKQGANLDYIRDYNMLGDGQYINIGAKPPRPAAGKAFID
                     FFLDEESMKIQADTGEFVNRKGVYPPLQDADKIKFVQMYQFTKEDFEIKKKEYQKIFF
                     Q"
     gene            complement(2536..3519)
                     /locus_tag="VMZ02_08490"
     CDS             complement(2536..3519)
                     /locus_tag="VMZ02_08490"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72012.1"
                     /translation="MLKKALSICFLCLLLAATVRAQETNLVAYAGFAGFQAPVWAPKD
                     LGLFAKYGFNGELVFISGSVRQIQAILGNSIQFAQADAATALNAIHNGADLVILSGSL
                     NSFPYSFVTSKEIRKPEDLSGKKIGILAVGGATETATFLALKAWNLPRQSVTIFPAGD
                     SAARIIALSAKAIDGTMLSYPDINEALRLGLHEFAKMSDVKGSSFPMNVMVARRSWAE
                     KNRDAVKRFQQAYAEGVYQFMNNKDRGLAVLGKRLRFKNPKAIEETYQYFAKSFAFPT
                     RVSHEGLRNTLEIIAQRNPAAKVDTNPAKYLDESTLDELEREGFFKRFASK"
     gene            complement(3619..4368)
                     /locus_tag="VMZ02_08495"
     CDS             complement(3619..4368)
                     /locus_tag="VMZ02_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR72013.1"
                     /translation="MKNDNRQTAVDSIRVIEGELRIACKILEWEIGDIWGHVGVRLPD
                     DKGIAVQMFRRAEEGNKDWLVHFDYALKKLAGVGTPPRESVIYSEIFKARPDVNATVH
                     CHASTCVAMSLADTPVSCVHVQSGRFGRGVPIYPQPIYILDESEGADLARTLGDAKGM
                     MIKGHGIVAVGASIDEACMNAVYMERTAKIMATAKLFGFTKVPDDFVETLAGSKEKLL
                     TRGKRLAHSAEWNYYAEKIKRGETWNRGGGA"
     gene            complement(4388..5671)
                     /locus_tag="VMZ02_08500"
     CDS             complement(4388..5671)
                     /locus_tag="VMZ02_08500"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR72014.1"
                     /translation="MLSREDNDLLTQTDQGTPMGNLIRRYWIPALLSEEIPRNDCPPV
                     RVKLAGEKLVAFRDSQGRIGLIGEHCAHRGTSLFFGRNEECGLRCIYHGWKYDADGNV
                     LDTPAEPSGSDFAKKLRHTAYPTHEANGVIYAYLGPREKMPLFPNYEWTQVPLEYSYV
                     TKCLLECNYLQGLEGECDSSHLSFLHRSFTNESNQPLYKGDPAPFYDTEDTDFGVRLI
                     ATRNFEDQHYVRFSAFVMPVYGCVPAGRKTNELEGYEIHTYVPADDTHCWRYDIGFHR
                     GRPVRDDEVHRRKQINPDYTKVRNARNNYLQDRQKQKTSTFTGIEDFLNHDGCATESM
                     GAIFDRTKEHLGVSDKAVIAVRRFLLTTVKELHSGKEPPHLVRDPVQNWFPHIDCFAH
                     LVPRNIPWQKKFDFLTEAAQKENPMSYAARKKAAS"
     gene            complement(5807..>6262)
                     /locus_tag="VMZ02_08505"
     CDS             complement(5807..>6262)
                     /locus_tag="VMZ02_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72015.1"
                     /translation="ELDAMITDISDTTMFENLEHHPKIKRLFPDYLQEDLDLYNDTGI
                     FTPVHMIVMSRKLERERPDLAGKFYAAFEKAKALAYEDILSDRGGFSVVYLREQLKAQ
                     MAQWGDPWKYGIKANQTSIDAFIKYNVEQGMIRKAPTYADIFAAGTLNT"
BASE COUNT         1270 a         1629 c         1816 g         1547 t
ORIGIN      
        1 atccaggtgt cttcgaaatt cctggattcc gggtcgcccc cggccttcgc cgggattgct
       61 cggaatgacg attcaattta gcgcgaactt tgaaggtact gcaggcgttt cagcgcctgc
      121 gctaccgcta ctttgagagc attaagcagg ctcgactgag gcacgggctc tttttgaaca
      181 tctttatttt acttcgcctt cttcaccgcc gcgatatcgc cttcgctgaa gcggtagaat
      241 ctcaccgggt tgtcgtagag catcttttgt ttgaactcat cggacaaatc cttgcgcgct
      301 tttaagtgag gcaggtcgcc gccgaattgg tcgcgttcgc gctcgtgcgg gaagtcggag
      361 gcccacatga tgacgtcgtc gcgaatcaaa tcagcgaggt aaggagcagt tcgttcttcg
      421 acttccatgg tgacgaagat attccccgac ttgaaaaact tgctcggcgg cgacttcaaa
      481 ttgggcgcga ggcgcgcggc gaatttttcg tagtcttctt ccatgcgatc catcatgaac
      541 ggcacccagc cggcgccggc ttcgagatag gcgacgcgca gcttggggaa ttcctggaat
      601 atcccctgga acatcatgtt ggtgatgtga agaaataggc caaccggatg ttccaacata
      661 tgcgcttcga gaaaactggc gacgcggtcg aggccgagat tctggctgac gccgccgtgg
      721 atggtcagcg gcacatctaa cctctgcgct tcatcgtaga tttcgtgaaa caccggcgaa
      781 ccgtagagcg gtgtgcgatt attcgttgcg gacggaagta ggccggcgac gaaaccaaga
      841 tccttgacgc agcggcgcag ttcggcggcc gcggctttcg ggtcttgcgg agctagcaga
      901 gcgacgcctt tcaaacgcgg cgactgggac ataaaacgat cgtaaagcca atcgttgtaa
      961 ccttgtgcga gcgcggaagc ccacgcgggc agttgaatca tgcccaaggc gagcgccgat
     1021 gttggataga gcacggccat gtcgatgccg tgctcgttct gaaatttgat ccagcgctcg
     1081 gcgtcgggat cttcccgctt gcctttgcgc gctaggccgc gctgccagcc gtcggcgtcg
     1141 aacatcggcc aggtcgaatg ccagttcatc tcggcgaagt tgcctttgat gtgggcgaag
     1201 atatctttgt ggtcttccat gatatggccg tcggcgtcga tgatgaattc tgccatggtt
     1261 cctccgaatg cgttgcttca gtcgctagaa atggatttct ccctgcggtc gaaatgacag
     1321 agtagcaatc ttgtcatttc gagcgagcgt cgcgagggag aaatctgggg ggttcagggt
     1381 gtctcccaag cgcttgcgtc cagcgagctc tattggaaga aaatcttttg atattctttt
     1441 ttcttgatct cgaagtcttc tttagtgaac tggtacatct gcacgaactt gatcttgtcg
     1501 gcgtcctgga gcggcggata gacgcctttg cgattgacga actccccggt gtctgcctga
     1561 attttcatgc tttcttcatc caagaaaaaa tcgataaatg ctttgcctgc tgcggggcgt
     1621 ggtggtttcg cgccgatgtt gatgtactgg ccgtcaccca acatgttgta atcccgaatg
     1681 tagtcgagat tggcgccttg cttgttgtag gtcaaaacga agcgcgcgaa ggtgatgccg
     1741 atgggaactt caccggtgcc gaccttgtcg gcggccggca cgatcgattc ggcgaatacc
     1801 ggttttgccg cggccaagtc gcggacaaat ttttccacgc gctgcttcgg catgattttg
     1861 tccaagctgg cgagccattg cgtcgtcaga gtatgtccag ttggatgggg catcactaat
     1921 ttgccgcgat actccggctt gacgatatct tcgagggtct tggggtattc gccggccttg
     1981 agttgagctt tgttatagat cactccgtac atgatgttgc ggtagcgagc gcccaggacg
     2041 ggatcgatgg cttctttggc gaaggctttc agggaaggcg agtcatattt gccgatggca
     2101 ccttcgtttg ccatcagacg catgtgatca cccgtgatgg cgactacgtc gaagcccggt
     2161 ttaccgaccc gatgttcgct caacacgcgg tcgagaactt cggttgaacc gccgcgccag
     2221 tagtccatct gcaaaccggt tcttttttca aaggcggctt tgagcgcgtc ggcggtaaag
     2281 gtttccatcg acgtgtagac gatgactttg ccgccttcct tctttgctgc ggcgacggcc
     2341 tgcgcagact gagcgtaggc ggagtgtcct tgcacgagcc ccaagcatac gaacagaaga
     2401 gctgaaagtt tacgcatcgc aagacttcct ttctcccatc acaagttcac acttttcacc
     2461 ggccagttat agggtacggg tgaacatcga gtcaagatcg tccgagtcat gagcggtgtc
     2521 gatgaggctt caggatcatt tactcgcgaa tcgtttgaaa aaaccttcgc gttccaattc
     2581 atcgagagtc gattcgtcga gatatttagc cggattcgta tccactttcg ctgcgggatt
     2641 gcgttgggcg atgatttcca aggtgttacg caacccttcg tgggagacac gggtgggaaa
     2701 ggcgaaactc tttgcgaagt attgatatgt ttcttcgatg gccttggggt ttttgaagcg
     2761 cagccgcttg cccagcacgg cgaggcctct gtccttgttg ttcatgaact gataaacgcc
     2821 ctcggcgtag gcttgctgaa agcgcttcac ggcgtcgcga tttttttccg cccagctgcg
     2881 gcgcgcgacc atgacgttca tcggaaacga cgagcctttg acatcgctca tcttggcgaa
     2941 ctcatgcagg ccgaggcgga gcgcttcgtt gatatccggg taggaaagca tggtgccatc
     3001 gatcgctttg gccgatagcg caataattct tgcagccgag tcgccggcag gaaagatcgt
     3061 cacggactgg cgcggtagat tccacgcctt caaggcgaga aaggtcgcgg tctccgtggc
     3121 gccgccgacg gcgaggatgc cgatcttctt gccgctcaaa tcttccggtt tgcgaatctc
     3181 tttgctcgtt acgaagctgt acgggaagct gttgagcgag ccggacagga tcaccagatc
     3241 ggcgccgttg tgaatggcgt tgagtgcggt cgcggcatcg gcttgggcga actggatgct
     3301 gttgccaaga attgcctgaa tctgccgcac ggagccggaa ataaatacca gttcgccgtt
     3361 gaaaccgtat ttggcgaaca gccccaagtc ttttggcgcc cagaccggcg cttgaaagcc
     3421 ggcgaagccg gcgtaagcaa ctagattagt ctcctgggcg cgcactgttg cggcgagaag
     3481 gagacagaga aagcagatgg acaaggcttt tttcaacatt gtctgtctcc tataaagaag
     3541 tcgcaacagg aaagcggttc gatgagtgtt agtaagaagt ttgcaatttc attgcgatgt
     3601 aaaagctcac agctgaacct acgcgcctcc gccgcggttc caggtttcac ctcgtttgat
     3661 cttctcggcg taatagttcc actcggcgga gtgtgccaaa cgcttgccgc gtgtaagcaa
     3721 tttctctttg ctgcccgcga gtgtttcaac gaaatcgtcc ggcacctttg tgaagccgaa
     3781 gagtttggcc gtggccatga ttttcgccgt gcgctccatg tagacagcgt tcatgcaagc
     3841 ttcgtcgatg gatgcgccga cggcaacgat gccgtggcct ttgatcatca ttcctttggc
     3901 gtcgcccaaa gtgcgcgcca ggtcggcgcc ttcggattcg tcgagaatat aaataggctg
     3961 aggatagatc ggcacgccgc ggccaaagcg gcccgattgc acatgcacgc acgacaccgg
     4021 tgtgtcggcc agactcatgg cgacgcaggt ggacgcgtgg cagtgcaccg tggcattgac
     4081 atcggggcgg gccttgaaga tttcgctgta gatcaccgat tcgcgtggtg gagtgccgac
     4141 gccggcgagc tttttcaacg cgtaatcgaa atggacgagc cagtctttgt taccttcttc
     4201 ggcgcggcga aacatttgca cggcgatgcc tttatcgtcc ggcaggcgta cgccgacgtg
     4261 gccccaaata tcgccgattt cccactcgag gattttacag gctatacgca gttctccttc
     4321 aatcactcta atgctgtcaa cggcggtttg ccggttgtca tttttcattg atcactccag
     4381 gtgcgcatta tgacgcagct ttcttacgcg ccgcgtagga catcgggttt tctttctgcg
     4441 ccgcttcggt caagaagtcg aacttctttt gccaaggtat attgcgcggc accagatggg
     4501 cgaagcagtc gatatgcggg aaccagtttt gcactggatc gcgcaccagg tgcggcggtt
     4561 ctttgccgct gtgcagctcc ttgaccgtgg tgagcagaaa acggcgcaca gcgatgaccg
     4621 ctttgtcgct gacgccgaga tgttctttgg tgcgatcgaa gatcgcgccc atgctctcgg
     4681 tggcgcagcc gtcgtgattc aagaaatctt cgatgccggt aaatgtcgag gttttttgct
     4741 tctgccggtc ctgcaaatag ttgtttcgcg cattgcgcac tttggtgtag tcgggattga
     4801 tttgtttgcg ccggtgcact tcatcgtcgc gcacagggcg gccgcgatga aagccgatat
     4861 cgtagcgcca gcagtgggta tcgtcggccg gcacataggt gtgtatttcg tagccctcga
     4921 gttcgttggt cttgcgtccc gcgggcacgc agccgtagac gggcatgaca aaagccgaaa
     4981 agcgcacata gtgctgatcc tcgaaattgc gcgtagcaat caagcgcacg ccaaaatcgg
     5041 tatcctcggt gtcatagaac ggcgccgggt cacctttgta gagcggttgg ttactttcgt
     5101 tggtaaagct gcgatgcaaa aacgataggt gcgatgaatc gcattcgccc tcgagtccct
     5161 ggagataatt acattccaat aagcacttgg tcacatacga atattccagc ggcacctggg
     5221 tccattcata gtttggaaac aacggcatct tctcgcgtgg gccgagatac gcataaatga
     5281 caccgttggc ttcgtgggtc ggataggcgg tgtgacgaag tttcttggcg aagtcgctgc
     5341 cgctgggctc cgccggcgta tccaaaacgt tgccgtcggc gtcgtacttc cagccatgat
     5401 agatgcagcg caggccgcac tcttcgttgc ggccgaagaa aagcgacgtg ccgcggtggg
     5461 cgcagtgctc gccgatcagg ccgatgcggc cttggctgtc gcgaaacgca actagcttct
     5521 cgcccgccaa tttgactcgc accggtgggc aatcgttgcg cggaatctct tcggacagta
     5581 gagcgggaat ccaatagcgc cggatgagat tgcccatcgg tgtgccctga tcggtttggg
     5641 taagtaggtc gttgtcctct cgcgatagca ttctagctcc tcagtgattg ttcaatggtt
     5701 caagcgttca acctcggact gttcaagccg ttgaaaaagt tctaatcgtt gaagtcgtcg
     5761 gaccaaattc gaaaaacttt gcgccaaagg ttactacgta tcattgttag gtgttcagcg
     5821 tgccggcggc gaagatgtcg gcgtaggttg gagctttgcg gatcatgcct tgctcgacgt
     5881 tatatttaat aaacgcgtcg atcgatgtct ggttggcttt gataccgtac ttccatgggt
     5941 cgccccactg ggccatttgc gcctttagtt gttcgcgcag ataaacaacc gaaaaaccgc
     6001 cgcggtcgct cagtatgtcc tcgtaagcca atgccttggc tttttcaaaa gcggcgtaga
     6061 atttaccggc gagatccggc cgctcgcgct caagtttacg gctcatgacg atcatgtgca
     6121 caggcgtgaa gatgccggtg tcgttgtaaa gatcgaggtc ttcctggaga tagtcgggaa
     6181 atagacgctt gatctttgga tgatgttcca gattctcaaa cattgtcgta tctgaaatgt
     6241 cggtgatcat cgcgtctagt tc
//
LOCUS       DATLCH010000290         6201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7085411, whole genome shotgun sequence.
ACCESSION   DATLCH010000290 DATLCH010000000
VERSION     DATLCH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7085411"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..700)
                     /locus_tag="VMZ02_08510"
     CDS             complement(335..700)
                     /locus_tag="VMZ02_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72016.1"
                     /translation="MLNAVFLASALLFPAFANAQAVNFKEIEAKVREFRGMLDQAGVN
                     GTRFWTRLDSSKRPHRLYVGDGFMTADEDAKENFIEIFSRFLAGHPEKNMLIDIYDGS
                     TSKLIGEYGFGGFRLFPAK"
     gene            complement(738..1463)
                     /locus_tag="VMZ02_08515"
     CDS             complement(738..1463)
                     /locus_tag="VMZ02_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUR72017.1"
                     /translation="MTNSNKYLALTALGFFMTGCTGMTKTQAGLVGAAVCGVAGGGGG
                     AAAAHNHVEGKHRNEAVGAAIGAVTGALLCGGLAYLFTENPKPPPPPPPPPPPPPPPP
                     PPPPAKKEAPPPPPPPPPPPPKPAPKVERTIILDDVLFDFDKSNVKPEAAAILDRLVA
                     FMKENSDKKANLSGHTDNVGTDAYNQALSERRVASVKDYVVGKGVDGGRVSGQGFGES
                     KPIADNKTAEGRSKNRRVEIKVN"
     gene            2038..4935
                     /locus_tag="VMZ02_08520"
     CDS             2038..4935
                     /locus_tag="VMZ02_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72018.1"
                     /translation="MASPARRIVIILAFFAAGLIGSAIALSFGVHSDGFRRWLQANLS
                     QRTGYAIAIGRLRLALPLRLVASDVTVDKDGKRILSAGEFKVAVSPLDVFAKTIHNVE
                     IERPVLEMDLAEILKSGPKDKTNLSLRNLKISNGLAVIRTEAGSRLEIPGITLSAQNL
                     NLGGQTGITLRAEILSLDGVADIAIERRDREFSLRATMNAKPTTGLLGRGVSANAQPL
                     VKADGTLRMPLGQEPTIEAALKFNRLKLGGRELTGELKTIATLDKNFAAGTFSSQLGV
                     NGFPNIISPVPLQIRDEPALLNAHGGFSVSDKSIALKSLTLHSHLGNAEAIGNLVFTP
                     VLSVNDGHIAAHRLDWQIVKTSLPEPFNQWIYQGQGELEMKLRGPWNALQFEGLARSE
                     NTQVRGANFSLGSFSLRAPFKWANGQLAVQNGRIDAKKFSLEGKLRSGVAQAEIGAIS
                     YDPKKPGLVTAQIKLSGGQFSSADSSKVGEDLALEGSVEIIAQKQRKVSSVLARLSFT
                     AGELLWGKFFGELKAQKPVLVIDAEYDRDTDRLQCHSCTIALATVGQMNLAGSIETIS
                     QTPQLRLQARSDNLSPAGFFQYFLRETYHRQYPLLDKLTVGGQMAMQLQLDGAPEQLA
                     LSGNLSLSNGEIASLSNDWQLAAMSINLPFQISLDDRPLENAAAPVLGSLSIERARFG
                     NQQLGPLTTTISLSNNALRLHQPLHLSLFGGAVELRNVTWRDIVRDPKAVTFSADTKR
                     LQLQELTAALNWPRFAGQLDGAIPEVESVGNTLRSRGEIQADLFGGRLRLSKLEIENL
                     FSELPSIKMSAKLESIDLEQLSKTFAFGRISGILEGTIEDLVITDGQPSQMRADLHSV
                     QRSGVDQRISVDALNKITVLSSGQEAGALYGGLAGFFDSFRYSKLGFKAVLRNDRLTL
                     RGVESQGENELLVVGSFLPPTVNIVSHTQVIAFSELVRRLERIKTDKPAIK"
     gene            4948..5529
                     /locus_tag="VMZ02_08525"
     CDS             4948..5529
                     /locus_tag="VMZ02_08525"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HUR72019.1"
                     /translation="MLKRTAFILVFLVSACVTVNIYFPAAAVERAAEQIVKETWGDSS
                     KSPPPPAPQGQNRDSPWRLVMLGLVNEAHAQEADINVSNPSIRAIKDSIKARSEAIKP
                     FMDRGNIGIASDGLLTIRNSDGLNLKERAEIKQLVDAENRDRENLYGEIAKANNISSE
                     SIPKIKAIFAKSWLEQARPGWWIQDSQGNWRKK"
     gene            5574..6107
                     /locus_tag="VMZ02_08530"
     CDS             5574..6107
                     /locus_tag="VMZ02_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HUR72020.1"
                     /translation="MIRITDAIVIALVIALSGILTARTAAAQQIKGSYETTADVPLRE
                     GPGNFHKIITTLPKGVQINVVGKEGYWLRVESKHGSKPGYLDEQFARPIVAGAPTATP
                     SSQSPSTSVAGPYRTLRDIDLRETPNANARVVTRLPADLKVNVIRSEGDWLRVESQQG
                     GKPGYVDKREVERWKDR"
BASE COUNT         1477 a         1741 c         1658 g         1325 t
ORIGIN      
        1 tccatcgctt gcgccatcgg aaccgcagct ttcccggcac cgagaatata aatattcgga
       61 aagctggtga ataagtaggt tgtgtcagcg atttcgaggc cggtatcgct cacgcgcaca
      121 tggcgcctaa ccgcagcagc cgcatccact ctcttgactg cagcattaaa gattgcgagc
      181 gcatctgctc gcaaccgctc caatgaccgc tccatacgta gcgagcaggt tattgaaacg
      241 ttgccggcaa ctcaagttac caagcggaag ggtgatttat gcggataggg gaagactcaa
      301 gaagccgaaa cccgggaact tacagatgag aaccctactt tgcggggaag aggcggaacc
      361 caccaaaacc atactcaccg atgagcttac tcgtgctccc gtcatagata tcgataagca
      421 tatttttttc tggatggccg gcaagaaagc gggaaaaaat ctctatgaag ttttccttgg
      481 cgtcctcgtc ggcagtcatg aatccgtcac cgacatacaa tcgatggggc cgcttactac
      541 tgtcgagtct ggtccagaag cgagtaccgt taacgccggc ctgatccagc atcccacgga
      601 actctcggac cttggcttcg atctctttga aattgacggc ctgagcgttg gcaaacgccg
      661 gaaacaaaag tgcggatgcc aggaagacag catttagcat ctttcctggc atccgcatcg
      721 ttcaatcccg agagaaacta atttaccttg atttcaaccc gacggttctt cgaccgacct
      781 tccgcggttt tattatccgc gattggcttg ctctcgccga atccctgtcc agagacacgg
      841 ccgccgtcta cacccttgcc gacaacatag tccttgaccg acgcgacacg gcgttcagaa
      901 agggcttggt tgtaggcatc agtaccgaca ttgtcggtat ggccggaaag gttcgccttc
      961 ttgtccgagt tctctttcat gaaagcaacc aaacgatcca agatcgccgc agcttccggt
     1021 ttaacgttgc tcttatcgaa gtcgaagagc acgtcatcca ggataatcgt ccgctcaacc
     1081 ttgggcgccg gttttggcgg aggtggcggc ggaggcggag gtggcggcgg agcctctttc
     1141 ttcgccggag gaggtggcgg tggcgggggt ggcggaggcg gtggtggagg tggcggtgga
     1201 ggtggcttcg gattctcggt aaatagataa gcaaggccac cgcaaagcag cgcgccggtg
     1261 actgcgccaa tcgcagcgcc gaccgcttcg tttctatgct tcccttcgac gtggttatgc
     1321 gcagcggcag caccaccgcc cccgcccgcc acaccgcaaa ccgcagcacc cacgagcccc
     1381 gcttgagtct tcgtcatgcc cgtacaaccg gtcatgaaga atccgagcgc ggttaacgct
     1441 agatacttgt tgctattcgt catttctgcc tcccatttga aaaatcgcaa ttttcggcca
     1501 aattagtata agggttctaa gttgtcaagc aaaaatgcaa gtttctccca ggttgtcccc
     1561 taatgggagg cccgccgcca tgacgcctcg tcattgagcc tcgtataggt actggttcaa
     1621 cattccattg ctacctactg accgctggtt gaaccggccg gcgccgctgg tccactgcgt
     1681 cttaagggca ccggcagcgg cgacactcga ccattgaact atgacctatc gctgaccacg
     1741 ccaactgcga ggtcgacgac gttacctctc tgagtaattt cctttgcgaa tagagctgaa
     1801 ccgcggggct ggggcaaaga acccgtcgtt gcacggctct tgcggttcag tcgacgccag
     1861 ccgacaaagg acgacggttt aatatcaata tcgccgagaa aaaatgccta tcaattgcaa
     1921 atcgtgaatc ggtcatgaac aatctttgcg agccgatagt tttcaagcta accagaatca
     1981 tccgagcggc gagttttaac cgccgcaaag ctcaataact aacgaggcaa cgattacatg
     2041 gcgagccctg cccgcagaat tgtcatcata ctggcctttt ttgccgctgg cctaatcggc
     2101 agcgcgattg ctctgtcctt cggcgtgcat tctgacgggt ttcgccgctg gttgcaggcc
     2161 aatctgtcgc agcgtaccgg atacgccatc gccatcggcc gtttgagatt ggcactgccg
     2221 cttcgacttg tggccagcga cgtcactgtc gataaagacg gtaaacgaat tttgtccgct
     2281 ggcgaattca aggtcgccgt ttcacccttg gacgtctttg caaaaaccat ccataacgtc
     2341 gaaatagagc gaccggttct ggaaatggac ttggcagaaa ttcttaaatc cggacccaag
     2401 gacaaaacaa atctttccct gcgaaacttg aagatcagca atggtctcgc cgtgattagg
     2461 accgaggctg gtagccggct ggaaatacca ggaatcactc taagcgcaca aaacttaaac
     2521 cttggcggcc aaaccggcat cactttacgc gcggaaatcc tctctctcga tggtgtggcg
     2581 gatatcgcga tcgaacgtcg cgatcgcgag ttctccctgc gagcgacgat gaacgcgaaa
     2641 ccaacgaccg gattgcttgg acgcggcgtt tcggcaaatg cacagccact cgtcaaagcc
     2701 gacggcacgc tacgcatgcc gctcgggcag gagccgacta tcgaggccgc gctaaaattt
     2761 aaccgactca aattaggcgg cagggaactc accggcgaat tgaaaaccat cgcaacactg
     2821 gacaagaatt tcgcggccgg aaccttctcc agtcagcttg gtgttaacgg gtttcctaat
     2881 attattagtc ccgtaccgct gcagattcgc gacgagccag cgttgctgaa cgcccacggc
     2941 gggttttccg tcagcgataa atccatagcg ctgaagtcgc tgactctgca ctcgcatctg
     3001 ggcaatgccg aagctattgg aaacctcgtg ttcactcccg tgctaagcgt caatgacggc
     3061 cacatcgccg cgcatcgtct cgactggcag atagtgaaga catcactgcc ggagccgttc
     3121 aaccaatgga tttaccaagg ccaaggcgag ctggaaatga aactgcgcgg cccgtggaac
     3181 gccctgcagt tcgagggtct cgcgcgcagc gagaatactc aggttcgcgg cgcgaatttt
     3241 tccctaggaa gtttttctct tagagcaccc tttaaatggg ccaatggcca attggctgtt
     3301 caaaacggcc gcatcgatgc caagaaattc tctttggagg gaaaactcag aagtggcgtt
     3361 gcgcaggccg aaatcggcgc gatcagttac gacccaaaga agcccggcct agttacggcc
     3421 cagatcaagt tgagcggcgg ccaattctcc tccgcggaca gcagcaaagt tggcgaagac
     3481 ctagcactcg agggcagtgt agaaataatt gcccaaaagc aacggaaagt gagttcggtg
     3541 ctggctcggt taagcttcac cgcgggcgaa ttgttatggg gcaagttctt tggcgaactc
     3601 aaagctcaga aaccagtgtt ggtgattgac gccgaatatg accgagatac tgaccgccta
     3661 cagtgccaca gctgcaccat cgctttggcc acagtggggc agatgaacct agcggggagt
     3721 atcgagacga tttcgcaaac cccgcagttg cggttgcagg cgcgtagcga caatctgtcg
     3781 ccggcgggtt tttttcaata cttcctgcgt gagacttacc atcggcagta tccgctcctc
     3841 gacaaactca cagtgggcgg acagatggca atgcaattac agctcgacgg cgccccggag
     3901 cagctcgcct tgagcggcaa cctttcttta agcaacggcg agatagcctc tctctccaac
     3961 gactggcagt tggccgccat gagcataaat ttgccgtttc aaatcagcct cgatgaccgt
     4021 cccctcgaga atgcggccgc accggtttta ggctccctct cgattgaacg ggcgcgcttc
     4081 ggcaatcaac agcttgggcc gctgacgacg accatttcgt tgtcaaataa cgcgctacgc
     4141 ttgcatcaac cattgcatct ttccctgttc ggcggcgcgg tggagctgcg caatgtcaca
     4201 tggcgcgata tcgtccgcga tcccaaagcc gtgacgtttt cggccgatac caagcggctg
     4261 caattgcaag aattgaccgc ggcgttaaat tggccgcgtt tcgccgggca attggacggc
     4321 gccatccccg aagtcgagtc cgttggcaat acgctgcgca gtcgcgggga gattcaggca
     4381 gacttatttg gtggacggct gcgcctgagc aaactagaaa ttgaaaatct tttctcggag
     4441 ctgccgtcaa ttaagatgag cgccaaattg gaatcaattg acctcgaaca actgtccaaa
     4501 acatttgcat tcggtcgaat ctcaggcatt ttagaaggca cgatcgaaga tctcgtgatc
     4561 accgacggtc agccctcgca aatgcgcgcc gacctgcatt cggtgcaacg cagcggcgtc
     4621 gaccagcgca tcagcgtcga cgcgctgaac aagatcaccg tgctcagctc gggtcaggaa
     4681 gccggcgcgc tatacggcgg gctcgcgggt ttcttcgaca gttttcgtta cagcaagctg
     4741 ggctttaagg cggttttgag aaatgatcgt ctaaccctac gtggagtgga atcccaaggt
     4801 gaaaatgaat tgctggtcgt gggcagcttt ttgccgccga cagtcaacat cgtcagccat
     4861 actcaggtaa tcgctttttc ggagctggtg cgccgcttgg agcggatcaa aactgacaaa
     4921 cctgcaatta agtgatctgg aggtagcatg ctaaaacgca cggcatttat actcgttttt
     4981 ctcgtcagcg cttgcgtaac ggtgaacatt tactttcccg ccgccgcggt cgagcgtgct
     5041 gccgagcaga tcgtcaaaga aacctggggc gattcgtcaa agtcgccacc gccgccggca
     5101 ccgcaaggtc agaatcgaga ttcgccctgg cgcttggtca tgctcggtct cgtcaatgaa
     5161 gcccacgcgc aagaagccga catcaatgtg tccaatccat cgattcgtgc catcaaagac
     5221 tcgatcaagg cccgcagtga agcgattaag ccgtttatgg atcgcggcaa tatcggcatc
     5281 gcgtcggacg gtctgctcac catccgaaat tccgatggac tcaacctgaa agaacgggct
     5341 gaaatcaaac agttggtcga tgccgagaac cgcgatcgcg aaaatctcta tggtgaaatc
     5401 gccaaggcga ataacatttc cagtgagagc attccgaaaa tcaaagctat tttcgcgaaa
     5461 agctggctcg aacaagcgcg tccgggctgg tggatacagg acagccaggg taactggagg
     5521 aaaaagtaac tgaccggcaa tcgagacgtc caatagtttt tgaggaggca cacatgattc
     5581 gcattaccga cgccatcgta atcgctctcg ttattgccct gagtggaata ttgactgccc
     5641 gcacggctgc cgcgcagcaa atcaaaggct cctacgaaac caccgcggac gtgccgctgc
     5701 gcgaaggccc cggcaacttt cataaaatta taacgaccct acccaagggc gttcagataa
     5761 atgttgtcgg caaggaaggc tactggctta gagtcgaatc gaaacatggc agcaaaccag
     5821 gctacttgga cgaacaattt gcccgtccta ttgtggcggg tgctccgacc gcgacgcctt
     5881 caagtcaatc gccctctact tcggtcgccg gcccctatcg cacactgcgc gacatcgacc
     5941 tgcgtgaaac tcccaatgcc aatgctcggg tggtaactcg gctcccagcg gacctcaaag
     6001 tcaacgttat ccgctcggaa ggtgactggt tgagagtgga atcacaacaa gggggcaagc
     6061 cgggttacgt tgataagcgc gaagtcgaac gctggaaaga tcgctaatcc ggaaaccatc
     6121 gtaaatagag ctactcgagc caacctaccc cttccccagc tcctgcgatc ttcttgtcgc
     6181 attgaccacg gcatccataa c
//
LOCUS       DATLCH010000291         2689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10815073, whole genome shotgun sequence.
ACCESSION   DATLCH010000291 DATLCH010000000
VERSION     DATLCH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10815073"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..981)
                     /locus_tag="VMZ02_08535"
     CDS             complement(16..981)
                     /locus_tag="VMZ02_08535"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72021.1"
                     /translation="MLRIIVLLTMVVVGLLHAFVGGAMAEPIRIAMPSKSLTFLNYYL
                     ADKFGFFKNEGLEVSLPVGKADVQLAAVVTGDMDYIAAGGTVLRGATTGLPVKVVMVT
                     FDKPLFHLVARSEIKRIADLKGKAVAVTAIAATDAVGARVMAKAAGLNPEQDLTLIGA
                     GSTANAFAMLQGGGAAAAMLSIPFNFKAEESGYHSLGNLADHLRTVLAALGTSDARIK
                     SNPAQIKRVIRAALRGMEFARDQANQDKIIPFIMEDFQLDRKTADASLREIVKAFPKD
                     GMTSDEAIRVDIELMREQAKIKAPVATSQTLDYTLLKEVLAELKK"
     gene            complement(1116..2246)
                     /locus_tag="VMZ02_08540"
     CDS             complement(1116..2246)
                     /locus_tag="VMZ02_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72022.1"
                     /translation="MSEGNLPQFGINKNVKLVGRTDLAGGGQVVVENGYAYVGHMDPP
                     HGTTILDVRNPKHPKIVAQIEIPQGVHSHKVRVSGDVMLVNLERYRPKERLPTGLKIY
                     DIANREKPREIAFYETNGGVHRFTFDGRYAYLSVHVEGYIGRIPIILDLQDPAHPQEV
                     GRWWMPGQWTAGGEKPDWEGTAHQCHHPIRYGDRLYVSYWHGGFVILDISDMSKPRFI
                     SGLNWSPPYPSPIHTTLPIPWKLMGRDMMVVVDEEAGKRAPTPPAFMWMVDISDEKHP
                     VSVSTFNPEGAFNVAPGNQYGAHQPAEQVYDNKMFVTWFSGGLRVVDISNPYSLQEIG
                     YYVPNPGRGQKTVKSNDVYREANGLLYLIDRLDGLEILEYQA"
     gene            2450..>2689
                     /locus_tag="VMZ02_08545"
     CDS             2450..>2689
                     /locus_tag="VMZ02_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72023.1"
                     /translation="MGALKDLNPDLIPDGRGMSLASWPGAVMQDAMVKLVWANLLLQI
                     LDGVASYQILSTGVPEFNPLVSYYIENWGLMGGLFY"
BASE COUNT          557 a          738 c          758 g          636 t
ORIGIN      
        1 ggaggaatcg ttaagctact ttttcagttc ggccagcact tctttcagca gcgtgtagtc
       61 caaagtctgg ctggtggcga ccggggcttt gattttggcc tgttcgcgca tcagttcgat
      121 gtcgacgcgg atggcctcgt cggaggtcat gccgtctttt gggaatgctt tgacgatttc
      181 gcgcagcgaa gcgtcggcgg ttttacggtc gagttggaag tcttccatga taaagggaat
      241 gattttgtct tggttcgcct gatcgcgcgc gaactccata ccgcgcaggg ccgcgcgaat
      301 tacccgcttg atctgcgccg gattggactt gatccgggcg tcggaagtac ctaaagcggc
      361 cagcactgtg cgcaaatgat cggcgaggtt cccgaggctg tggtagccgg attcttcagc
      421 tttaaagttg aacggaatcg agagcatcgc cgcggctgct ccgccgcctt gaagcatggc
      481 gaacgcattg gccgtgctgc cggcgccaat cagtgtcaga tcctgctcgg gattgagccc
      541 ggccgctttt gccatgaccc gagcgccgac agcatccgtc gcggcgatgg cggtgaccgc
      601 caccgcttta cctttgagat cggcgatgcg ctttatttcg ctgcgcgcca caaggtgaaa
      661 cagcggcttg tcgaacgtca ccatcacgac tttgaccggc aaccctgtgg tcgcgccgcg
      721 cagcaccgtg ccgcccgcgg cgatatagtc catgtcgccg gtgaccactg ccgctaactg
      781 cacatcggcc ttgccgaccg gcagactcac ttcaagcccc tcgtttttga agaagccgaa
      841 tttgtcggca agatagtaat tgagaaacgt caggctcttg gacggcatag cgatgcgaat
      901 cggttcggcc atcgcgccgc cgacgaacgc gtgcaacagt ccaacaacga ccatggtcaa
      961 gagaacaatt atcctcaaca tggcgatcgg cttccatggg acttgatcac gaccggaaca
     1021 cattgcatgg gtattttcat attagaatcg taaaagtcca tagcgacggc gagtcatcac
     1081 cgctgttgcg caggattcga acattccgat ttccattaag cctgatactc gagtatttcc
     1141 agtccgtcca ggcggtcgat caaatagagc aagccgttgg cttcgcgata gacatcgttg
     1201 cttttaacgg tcttttgtcc gcgccccgga ttgggcacgt aatagccaat ttcctgcagg
     1261 ctgtagggat tgctaatgtc gacgacgcgc agaccgccgc tgaaccaggt aacgaacatc
     1321 ttgttgtcgt aaacctgctc ggcgggttga tgcgcgccgt actgattgcc gggcgcgacg
     1381 ttaaaggcgc cctctggatt aaacgtcgaa acagagactg gatgcttctc gtcgctgatg
     1441 tcgaccatcc acataaatgc cggaggcgtg ggcgcgcgtt tgccggcttc ttcgtcgacc
     1501 accaccatca tgtcgcggcc catcagcttc cagggaatcg gcagagtcgt gtgaatcggc
     1561 gacggatagg gcggcgacca gttgaggccg ctgataaacc gcggtttgct catgtctgaa
     1621 atatcgagga tgacaaaacc accgtgccaa tagctgacgt aaaggcgatc gccataacgg
     1681 atcggatggt ggcattgatg agccgtgcct tcccaatcgg gcttctctcc gccggccgtc
     1741 cactgtcccg gcatccacca gcggccaacc tcttgcgggt gcgccggatc ttgcaaatcc
     1801 aagatgatcg gaatgcgacc gatgtatcct tcgacatgga ccgataggta ggcgtaacgg
     1861 ccatcgaaag taaagcgatg tacaccgccg ttggtttcgt agaaggcgat ctcgcgcggt
     1921 ttctcgcgat tggcgatgtc gtaaattttt agtcccgtcg gcagtctttc ctttggccgg
     1981 tatctttcca gattaaccaa catgacatcg ccgctgacgc gaactttgtg cgaatgcacg
     2041 ccttggggaa tctcgatctg cgccacgatc ttcggatgtt tggggtttct gacgtcgagg
     2101 atcgttgtgc cgtggggcgg gtccatatgg ccgacgtaag cgtagccatt ctcgacgaca
     2161 acctgcccac cgccggccag gtcggtgcgg ccgacgagct ttacgttttt gttgatacca
     2221 aattgcggca gattgccttc actcataatc gtcatgctcc ttgcgccgga atagagacct
     2281 tgtaccaatc tcaagcgcaa gcggtcaaac gcaagcgatt tatcacatag agcgtaagtt
     2341 cttttaacac tttctcacta gtatgctttg catactgcga tgatcttacc gccggcaatg
     2401 cccggttggc aggcgagctt tccctgtgca ggcaagcctg atcatgtcca tgggcgcttt
     2461 aaaggacctg aacccagatc tgataccaga cgggcgcggc atgagtctgg caagttggcc
     2521 tggtgcagtg atgcaagatg caatggtcaa attggtctgg gcaaatttgt tgttgcaaat
     2581 cctcgacggc gtcgcctcat atcaaattct ttctaccggt gttcccgaat ttaatccgct
     2641 ggtgtcttac tacatagaga actggggact gatgggaggc ctgttttac
//
LOCUS       DATLCH010000292         7216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7832309, whole genome shotgun sequence.
ACCESSION   DATLCH010000292 DATLCH010000000
VERSION     DATLCH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7832309"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VMZ02_08550"
     CDS             <1..532
                     /locus_tag="VMZ02_08550"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HUR72024.1"
                     /translation="PDIKDSKMLSAQQREKLVPIIQESAQCWAVGMIDVEGIDRLNIL
                     RASLTAMAHALHGLSSQPDYAFIDGNQKIPLGIFAALGCKALRPTRQKTIVRGDQLCL
                     SIAAASIIAKVARDRIMIDLDKQYPEYGFAAHKGYGSAAHLAALQRYGPTPIHRRSFK
                     PVWDLLGSDQPDLFSD"
     gene            545..910
                     /locus_tag="VMZ02_08555"
     CDS             545..910
                     /locus_tag="VMZ02_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HUR72025.1"
                     /translation="MSLFRKRLGREGEELAAGFLRKKGYKLVERNYRCKTGEVDLIVL
                     DRKVVVFVEVKTRSDHSFGTPFEAVEARKQRKMIRAAQYFLHEKKLDQRDARFDVVGI
                     SWRGDQPLVEHIENAFEVS"
     gene            922..2193
                     /gene="serS"
                     /locus_tag="VMZ02_08560"
     CDS             922..2193
                     /gene="serS"
                     /locus_tag="VMZ02_08560"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HUR72026.1"
                     /translation="MLDVKLLRDDLAQVKERMATRGAAIDWDEFVAVDRERRDALASI
                     ERLKEKKNRLSGEIGKLKKSGVDASALMRETEEVSEAIRTGEAPLTEIEARFENFMLT
                     LPNLPSPNVKIGKSEHDNREVRRWGEPPKFDFEPQNHWDIGENLAILDFERAAKIAGA
                     RFTVYRGAGARLERALINFMLDLHIGENGYQEMLPPALVNRAALVGTGQLPKFEEDLF
                     HLAPGDYFLIPTAEVPLTNLHRDEILELESLPIKYVAYTPCFRSEAGSYGKDVRGLIR
                     QHQFNKVEMVKLTEPENSYDELEAMVRNAEEVLQRLKLPYRVVELCTGDMGFGAAMTY
                     DLEVWLPGQNTYREISSCSNCEEFQARRANIRYRKENKGKPLFVHTLNGSGLAVGRTF
                     VAVLENYQQKDGSVIIPEALRPYMGGLTQIS"
     gene            2279..2367
                     /locus_tag="VMZ02_08565"
     tRNA            2279..2367
                     /locus_tag="VMZ02_08565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2313..2315,aa:Ser,seq:tga)
     gene            2793..3905
                     /locus_tag="VMZ02_08570"
     CDS             2793..3905
                     /locus_tag="VMZ02_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR72027.1"
                     /translation="MAILKVAGVTKLYKDLKALDNVSFEFEKGILSFLGPSGCGKTTL
                     LRSIAGLEIPDAGSISIGDKVQTSIEGGVLIPPYSREIGFVFQNYALWPHMTVFKNVA
                     FALNLRKVPRDEVKRRVLAALELVGLGGREERYPSQLSGGQQQRVALARSIVMEPRLI
                     LLDEPLSNLDAKLREEMRVELKKLIKKVGISALYVTHDQEEAFVISDKVVVMNAGKIL
                     QYDSPDEIYSHPCDPFVASFIGHSTLVEGHVIKAVGGECVVQIPEFDNATLVCAAPKM
                     GLASGPCSVVIKNGDLRLSHQKFSIDAINVLEGAMTSREYRGGLTDHRVQIGSKEVVV
                     TSHRLCPMISVDGEGGKVFLCIEKSAISLIPTHAAV"
     gene            3937..5634
                     /locus_tag="VMZ02_08575"
     CDS             3937..5634
                     /locus_tag="VMZ02_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUR72028.1"
                     /translation="MTWKANRLALRQGSLFPLSASSLAAVVIVTPVLVVLWRSLTSGK
                     LGFTVAINVDNYLRVFADKDIWPMLSNSIIYAGGSALLGTGLGGLLAWIVARTNTPGK
                     GLVELMPLYPLLMPPIMKNIAWILLLAPKSGILNGMLEQFLGITYPVFNAFTMAGMIW
                     TFGIACVPLGYLFLLPVFLSFDPSLEESAYIAGSRPVSTMLKITFPLAIPAFISAFVL
                     NFLRALRSFETPVLQGTPGNIKVFVSRVYDSMALEFNTGLATAYSVVLVILSALTLYF
                     YVRATRFSERYATITGKGYRVRVIDIGPWKYVTFAAVCLYFLAGIAVPFVVLIVVSII
                     PYFDYETFMTFPAHMGLNNYRTVLRHASFLNGLRNSVMLSVVIALITVFAGIVMAFTI
                     HRTRAVGTKIFEFIGTLPLAFPPLVLSVGLVIIFVGTPLYNSLWALGVGLFVADFPYA
                     FRNASGAIVNIHKELDEAAWVHGAKWRHVFFKVTLPILKPAVGGALFYIFIEAIRNVD
                     VAVLLTAPGLEYGPVTLFEYFRVGQWAEASAGGVIYLIILIVAVSVAKFAFKMKFSL"
     gene            5675..6838
                     /locus_tag="VMZ02_08580"
     CDS             5675..6838
                     /locus_tag="VMZ02_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72029.1"
                     /translation="MITQRPLCAALALSASVFVASALAAEKTPTAVKEAVKAAPAAAT
                     KAGGQQVKKEVGSATATAAAAKAFAQVSAELYPKAKQEGNLVVYSVWDVEHLRVITDA
                     FMKRYPGIKATYWQGRNPEIVTRVLTEFQGGQASFDVVLSDNAPPVIRAAGGIMPYET
                     VQKDVLYLHDPTLPTVSLQIQALSYNTRKMKPADLPKSWEDVASPKYKGMIALDDPMR
                     AGPLSSMLAGLKTQWNDDARFNNFLKGLKALNVPVHKSTSAMFRLVISGEYNICMPAL
                     LHDVMEETHKGTPVGYVKTVPPVVFPRQAGIYAKSPNPNAAKLFAEWLISDEGQKTID
                     SIGRETSRNDFKSKTSIESAYGKGIKPIPVTDKGFLEDPKKWLDTNVKPIWEG"
     gene            7057..7147
                     /locus_tag="VMZ02_08585"
     tRNA            7057..7147
                     /locus_tag="VMZ02_08585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7091..7093,aa:Ser,seq:gct)
BASE COUNT         1574 a         1844 c         2054 g         1744 t
ORIGIN      
        1 tccggacatc aaagattcga agatgctcag cgctcagcaa cgcgaaaagc tggtgccgat
       61 cattcaagag tcggcgcaat gttgggcggt cggtatgatc gatgtcgaag gtattgatcg
      121 cctgaatatt ttgcgcgcca gcctcacggc gatggcacat gcgttgcacg gtttatcctc
      181 tcagccggac tacgccttca tcgatggcaa tcaaaaaatt cctttgggaa tttttgccgc
      241 gctcggctgc aaagcgcttc gtcctacgcg tcaaaaaacc atcgtcaggg gcgatcaact
      301 ctgtttatcc attgccgccg catcgatcat cgcgaaggtc gcgcgcgacc gaattatgat
      361 cgacttggat aagcaatacc ccgagtatgg tttcgccgcc cacaaaggtt atggcagcgc
      421 ggcgcattta gcggcgttgc aacgctacgg gccgacgccg attcatcgcc ggagttttaa
      481 gccagtatgg gacttacttg gtagcgatca gcctgacctg tttagtgact gacgataaac
      541 ccgcgtgagt ttatttcgca aacgcctggg tcgagaaggc gaggagctcg ccgcgggatt
      601 tttgcgcaaa aagggctaca aattggtcga gcgcaactat cgctgtaaga ccggcgaagt
      661 ggacttgatt gtccttgacc gtaaggtggt ggtctttgtc gaagtgaaga cccgttcgga
      721 tcacagtttc ggtactccct ttgaagccgt ggaggcgcgc aagcagcgta agatgatccg
      781 ggcggcgcag tattttcttc atgagaaaaa gctcgaccaa cgcgatgctc gtttcgatgt
      841 cgtcggcatt tcctggcgcg gtgaccagcc gttggtcgag catatcgaaa acgccttcga
      901 agtaagttag agaataaact catgttggat gtaaagttgt tgcgggacga tctggcgcag
      961 gtcaaggagc gcatggcgac gcgtggcgcc gccattgact gggacgaatt tgtcgccgtc
     1021 gaccgtgaac ggcgcgatgc cttggcttct atcgagcgct tgaaagaaaa gaaaaatcgc
     1081 ttgtcaggcg aaatcggcaa gctcaaaaag agcggcgtcg atgccagcgc actgatgcga
     1141 gagaccgagg aagtcagtga agcgatacgc accggtgagg cgccgctgac cgaaattgaa
     1201 gcacggttcg agaacttcat gttgaccctg cccaatttgc cgagtccgaa cgtcaaaatt
     1261 ggcaagagcg agcatgacaa ccgcgaagtt cgccgttggg gggagccgcc aaagttcgac
     1321 ttcgagccgc agaaccattg ggatatcggc gaaaatttgg cgatcctgga cttcgagcgc
     1381 gcggctaaaa ttgccggagc gcgttttacc gtatatcgcg gtgccggcgc taggttagag
     1441 cgcgcgctga tcaattttat gctcgatctt cacattggcg aaaatggtta tcaggaaatg
     1501 ctcccaccgg cgctggtaaa tcgagccgct ttggtcggta ccgggcagtt gcccaagttc
     1561 gaagaagatt tgtttcacct ggcgccggga gactattttc tcatccccac cgccgaagtt
     1621 ccgctgacca atctgcaccg cgacgagatt ctcgaactcg agtcgttgcc gattaaatat
     1681 gtcgcctaca cgccatgctt ccgcagtgaa gccggatcct acggcaagga tgtgcgtggg
     1741 ttgatccgac aacaccagtt taacaaggtc gagatggtta agttgaccga gccggagaat
     1801 tcttacgatg agctggaagc aatggtgcga aacgccgaag aagttttgca gcggctaaag
     1861 cttccttatc gggtcgttga gttgtgtacc ggcgacatgg gcttcggcgc ggcaatgact
     1921 tacgatctcg aagtctggct accgggacaa aacacgtatc gcgagatcag ctcttgttcc
     1981 aattgcgaag agttccaagc gcgccgagcc aacattcgct atcgcaaaga aaacaagggc
     2041 aagccgcttt tcgtgcacac gctcaacggt tccggtttgg cagtcggccg cacattcgtc
     2101 gctgtgctgg aaaattatca gcagaaagac ggcagcgtga ttatcccgga agcgctccgg
     2161 ccttatatgg gcggactgac gcagattagt taattctgtt gttgtaacgc attttgctag
     2221 tcgttatcct atcgttggct cgaggttttc gacctcgagt cttaccaact caattctcgg
     2281 aggggtggga gagcggttta atccaccggt cttgaaaacc ggcgcccact tcgtgggccg
     2341 cgagttcaaa tctcgccccc tccgccatta tttcacagga ccgtcggccg aagggcttgt
     2401 aactgattgc gcatcttgtc tgtctctctg cattgctatt agaaatccag cgtcgccttc
     2461 ggtaatttca aatcctctgg tgctgccaaa tacggatccg gctgagccgg gaaacaccta
     2521 aagtgtgagt gccttacagt tgacggagtt tcttagtgga tgaacgtgcc ctctggctgt
     2581 ttgtgcccaa cctctggcgt tggctgctca ggaatgcatc acagggtgat ggttatactt
     2641 gagttttacc ggcatcgtga tcggctgagg gtgtggaatt tgtgattatc tcctgaggac
     2701 gtgtttaatt tcatcgatct ttggagtaag agtgtctacg ccgtgaattg gcggcgtatc
     2761 tattgcattg agcacagctt tcggattcat ccatggccat tttaaaagtt gccggcgtca
     2821 ccaaactcta taaagatctc aaggcgctcg ataacgtcag tttcgagttt gagaagggga
     2881 ttctctcgtt tctcgggccg tctggatgcg ggaagacgac tttgctgcgt tccatcgccg
     2941 ggctggaaat tcccgatgcc ggctcgattt ccatcggcga caaggtccag acctccatcg
     3001 agggtggtgt gctcattccg ccgtattctc gcgagattgg cttcgtcttt cagaattatg
     3061 cgctgtggcc gcatatgacg gtattcaaaa atgtcgcctt cgcgttgaat ttgcgcaagg
     3121 tgccgaggga cgaggtgaag cggcgcgtac tggccgcgtt agagttggtc ggcttgggcg
     3181 gtcgcgagga gcgttaccct tcgcagttga gcggcggtca acagcagcgc gtcgcgttgg
     3241 cacgtagcat agtcatggag ccgcgcttaa tattactcga cgagccgctc agtaatttgg
     3301 acgccaagct gcgcgaggaa atgcgcgtcg aactaaaaaa actcatcaaa aaagtcggca
     3361 tcagcgcgtt atacgtgacc cacgaccagg aagaggcatt cgtcatctcc gacaaggttg
     3421 ttgtgatgaa tgccggcaag attctgcagt acgattcgcc cgacgagatt tacagccatc
     3481 cgtgcgatcc gtttgttgcg tcattcatcg gccattcgac attggtcgaa ggccacgtga
     3541 tcaaggcggt gggcggagag tgcgtcgtgc agatcccgga gttcgataat gcaacgcttg
     3601 tttgcgctgc ccccaagatg ggtttggcga gcggaccttg tagcgtggta atcaagaatg
     3661 gtgatctgcg attgagtcac caaaagttct ccattgatgc catcaacgtt ctcgaaggcg
     3721 ctatgaccag ccgtgagtac cgcggcggcc taactgacca ccgagtgcag atcgggtcca
     3781 aggaagtggt tgtgacctcg caccggctct gtccgatgat cagcgtcgac ggcgagggcg
     3841 gcaaagtttt tctttgcatc gagaaatcgg ccatcagtct catccctaca cacgcagccg
     3901 tctagcgggc atgaacgacc gaggagaatt gctcgcgtga cttggaaagc aaaccggctt
     3961 gccctgcgcc agggaagtct tttcccattg tctgccagtt cccttgccgc tgttgtgatc
     4021 gttacacctg tcctggtggt tttgtggcgc agtttgacca gcggcaagtt aggcttcacg
     4081 gtcgccataa acgtcgataa ctatctgcgg gtatttgccg acaaagatat ttggccgatg
     4141 ctcagcaatt caatcatcta tgctgggggg tcggcgctgc taggcacagg actgggcggc
     4201 ctgttggcat ggattgtcgc gcggaccaat acaccgggca aaggcttggt cgagttgatg
     4261 ccgctctatc cactattgat gccgccgatc atgaagaata tcgcctggat tcttttgctc
     4321 gcgccaaagt cgggtatttt aaacggaatg ctcgagcaat ttctggggat cacttatcca
     4381 gtcttcaatg cctttacgat ggcggggatg atctggactt tcggtatcgc ttgcgtgcct
     4441 ctgggctatt tgttcctgtt accggtattc ttgtcattcg acccatcgct ggaagaaagc
     4501 gcctatatcg cgggcagccg accggtgagt accatgctca agatcacttt cccattggcc
     4561 ataccagctt ttatttccgc tttcgtattg aactttttac gcgcgctgcg ctcgttcgag
     4621 acgccggtat tgcagggaac gccgggtaac atcaaagttt ttgtcagccg cgtttacgat
     4681 tcgatggcgc tcgaatttaa taccggactg gcgacggcgt atagcgtagt gttggtaata
     4741 ttgtcggcat tgacgcttta cttttatgtg cgcgcgacac gcttctccga acgctacgcg
     4801 acgatcaccg gcaaaggata tcgggtgcgt gtcatcgata tcggtccgtg gaaatacgtc
     4861 acctttgcgg ccgtctgttt atattttctt gccggtatcg cggttccctt tgtagtcctc
     4921 attgtcgtgt cgatcatccc gtatttcgac tatgaaactt tcatgacctt tccagcgcat
     4981 atggggttga acaactatcg caccgtgctg cgccatgcga gcttcctgaa tgggctgcgc
     5041 aattcggtca tgctatctgt ggtaatcgcc ttaatcaccg tgttcgccgg catcgtcatg
     5101 gcatttacga ttcatcgcac tcgtgccgtc ggcacaaaga ttttcgaatt tatcggcacg
     5161 ttgccgctgg cctttccgcc gttggtgctg tcggtgggac tggtgatcat cttcgtcggg
     5221 acgccgctct acaacagcct ctgggcgctt ggtgtgggtt tgtttgtcgc cgatttccct
     5281 tatgcattcc gcaacgcgtc aggcgccatt gtcaacattc ataaggagct cgacgaggct
     5341 gcatgggtgc atggagcgaa gtggcgccat gtttttttca aggtcacttt gccgatcctc
     5401 aaacccgcgg tcggcggcgc gcttttttac attttcatag aagcgattcg taacgtcgat
     5461 gtggcggtgc tattgaccgc gccggggctg gaatatggac cggttacttt attcgaatac
     5521 tttcgcgtcg gccaatgggc ggaggcgtcg gccggcggcg tgatctattt aattatcttg
     5581 attgttgccg tatctgtagc taaattcgcg ttcaaaatga agtttagtct gtgagggttc
     5641 gtcaaaacta cgattcagca gaaggggagg attcatgata acccaacgtc cgttatgtgc
     5701 ggctctcgca ttgtcggcga gtgtctttgt agcctcagcg ctagccgccg agaagacacc
     5761 gacggcggtc aaggaagcag ttaaggcggc accggcggcg gcgacaaaag ccggcggcca
     5821 acaagtaaag aaagaggttg gcagcgccac tgcgacggca gctgctgcga aagcttttgc
     5881 ccaggtgagc gccgagctct atccaaaagc taaacaggaa ggcaacttgg tggtctattc
     5941 ggtgtgggac gtcgagcatc ttcgcgtcat caccgatgcc ttcatgaagc gctaccctgg
     6001 tattaaggcc acctactggc agggacgcaa tccggaaatc gtgacgcggg tgcttacaga
     6061 atttcagggc ggccaagcga gcttcgacgt ggtgctttcg gacaatgcgc cgccggtcat
     6121 tcgagcggct ggcggcatca tgccctacga gaccgtgcag aaagacgtac tatacctgca
     6181 cgacccgaca ttgccgacgg taagtttgca gattcaggcg ctgtcttaca atacgcgcaa
     6241 aatgaaaccc gcggacttgc cgaagagctg ggaagatgtc gccagtccaa agtacaaagg
     6301 catgattgct ctcgatgatc cgatgcgcgc cgggcctttg agttcgatgc tggcggggct
     6361 gaagactcag tggaacgatg acgcccgctt caacaatttc ctgaagggat tgaaggccct
     6421 gaacgtgccc gtgcacaaga gcaccagcgc aatgttccgg ctcgtgatct cgggcgagta
     6481 caacatctgc atgcccgcgc tgctgcacga cgtcatggaa gagacgcaca agggtacccc
     6541 ggtcggttat gtcaaaacgg ttcccccggt ggtattcccg cgtcaggcag gcatatacgc
     6601 caagtcgcct aatccgaacg ccgcgaagct atttgccgaa tggctaattt cggatgaggg
     6661 gcaaaagacc atcgattcaa tcggccgaga gacttcgcgc aacgacttca aatccaagac
     6721 ctctatcgaa tccgcttatg gtaaagggat taagccgata cccgtgactg ataaaggatt
     6781 tttagaagat ccgaagaagt ggctcgacac taacgtaaag ccaatttggg aaggctgatc
     6841 cgaaagtggg cgcaaaaagg gggcgggcgc aacgcgcctc gccccttttt tttcgaagcc
     6901 gaatgtttgg aagccaagac gcggcacgga ttgtagcaaa gctttttctc gtgtgttagt
     6961 ttgaccgggc ttgggagccg cccgagtaac ggtaaatcga caaaatttaa ctgcgcgatt
     7021 ctccggctcc tgccggacgc gagattggac cggcacggag agatggccga gtggtctaag
     7081 gcgcacgcct gctaagcgtg tgtaccccaa aagggtaccg agggttcaaa tccctctctc
     7141 tccgccattt cttattctga cttaaagatt ttcttgtgca ttgtcggcat tgtggctggc
     7201 gcacttagtt gtatct
//
LOCUS       DATLCH010000293         6424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7107475, whole genome shotgun sequence.
ACCESSION   DATLCH010000293 DATLCH010000000
VERSION     DATLCH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7107475"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VMZ02_08590"
     CDS             <1..696
                     /locus_tag="VMZ02_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HUR72030.1"
                     /translation="DNCRRGLARHCPDRRVMGIVNADGAFAEYVAVPVANLHRVPDSI
                     TDEDAVFTEPLAAAFEILTQIQINPGDDILVLGDGKLGNLCAQVLRLTGAKVTALGKH
                     ADKLAAIKRAGVRTVHLNDWKPRLFDIVVEASGSSSGLALALSAVRPRGTLVLKSTIA
                     GKHEVSLAPIVIHEINVIGSRCGPFPDALDALAMKRVAVAPLIEAIYSLQDGVDAVVR
                     AGEAGAKKVLLRP"
     gene            725..1474
                     /locus_tag="VMZ02_08595"
     CDS             725..1474
                     /locus_tag="VMZ02_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HUR72031.1"
                     /translation="MKWGEQRWPQMKALDRSKVVTILPTGSMEQHGPHLPLQVDHFIA
                     NRIAEDLEQRMPEILTLPPVWAGTSGHHMDFPGTITLRPRVFIGLLRDICTCMHAHGF
                     RRMILLNGHGGNRASLQVLGQELYAEMGFSINVIVYWDLVPELVKSLKKSKSKGMGHS
                     GELETSLMLYLAPHLVDLSAVPDGFRSDRLQHSRFISGEGITRYVNIKEHSDIGVDGF
                     PAAASAKDGELFYQTVLTEMGKVIRELQQED"
     gene            complement(1475..2074)
                     /locus_tag="VMZ02_08600"
     CDS             complement(1475..2074)
                     /locus_tag="VMZ02_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HUR72032.1"
                     /translation="MESVDSILTYWFGTNPDSAALAKDRADLWWSKNAETDREIRQRF
                     AVNVQLAAEGKLDAWLVEPRGRLAMIILTDQFPRSIYRDSPRAFAFDAKALEWTLDGV
                     ARGVDRLLRPIERVFFYLPLEHSERMADQERSVSALGELVPIVDVDQREIFEHYLNFA
                     ILHRDIIARFGRFPHRNKILGRPSTPEELYFLSQPGAAF"
     gene            complement(2080..2934)
                     /locus_tag="VMZ02_08605"
     CDS             complement(2080..2934)
                     /locus_tag="VMZ02_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72033.1"
                     /translation="MKLLCPAEVPISERDQVFSYSRAHALWAISLTAAGAGALSYLAW
                     LKQPEVGYFIFAVVFIFIFLFRKLLIARFHPANWLVRLSDDGLFIKFRSYLNNHFPEH
                     ELIVVFIAHAEIRSARYVVEKQQVPDRDGNKRPATSTKTNKLVELELAGKTKPLRDAL
                     QRERRYAMERKAQLAVSTRYQHQPVRLPTGDKLQIEWAVVPSAATFLNALSRHTPICA
                     AEKTSKDLLKLDEFSRAEQELRLLDLAESGDKIGAIATARRLYGYDLAQAKEFVEGLV
                     NKHSPYGS"
     gene            complement(2976..3725)
                     /locus_tag="VMZ02_08610"
     CDS             complement(2976..3725)
                     /locus_tag="VMZ02_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HUR72034.1"
                     /translation="MEFEAFAGLVSKSVKLVFFTGAGISTESGVPDFRSPGGVWTKYQ
                     PVLFQDFLASEAARLQHWQLKKATYELFKTVTPNVGHREICEFEQRGQLLGLITQNID
                     GLHKLAGTSDTRLVELHGTDRLVTCLKCGKAFQPSDVYETWHEALPTPTCDVCNGFLK
                     SANISFGQSMPVEAMQRAQRWSEGAEIFIVIGSSLQVQPAASFPVIAKRSGALLAIVN
                     RDPTPLDDLADFLHNGSIGEFFTQLKSLLGH"
     gene            complement(3743..4375)
                     /locus_tag="VMZ02_08615"
     CDS             complement(3743..4375)
                     /locus_tag="VMZ02_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HUR72035.1"
                     /translation="MQLNFIFRAIAAALLSFAIAQTAAAAEKAKNENVVKDGMLVSID
                     FTLKGTDGKVIDSSKGREPLKYVHGQKTMIPGLERELTGMKVGGEKNVTVKPDDAYGQ
                     INPNGFQEMPKETIPPNALKVGAVLQGQDDQGRPMVARIKEIKEKTVVIDMNHPMAGK
                     TLVFNVKVVDIQPAPPPTAPAAKSTAPAAKATEPPSKAATPAKPAEPLKK"
     gene            complement(4518..5498)
                     /locus_tag="VMZ02_08620"
     CDS             complement(4518..5498)
                     /locus_tag="VMZ02_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72036.1"
                     /translation="MRSSILHFPSSTLLLIGALLLAAEPVPAQLTKLRASFAGTTGYH
                     LPMWVQKQEGLDKKYGLDMEILLIAGGSRIIQTLLSGELLLTHSGAATVARAGIAGAD
                     LTMIATVMNQVNWRIVARKEIRDVKDLVGKKIAIASRGGSSELGLQLAFKKWNLDFNR
                     VSLLSLGPSPTRMAALREGVVDATVFAYPELLIATKEGFPTLADLRPYADLTDTSVVV
                     TRAALEKQRPLLKRFLQGYVEAIGRIKRNPDSAYRALARYTGVTDAAALEETRKFYAD
                     SFASVPRTEITGWRNLIATLGKNEAEMARFIDMSLLDELQREGFFDRIAK"
     gene            complement(5540..>6424)
                     /locus_tag="VMZ02_08625"
     CDS             complement(5540..>6424)
                     /locus_tag="VMZ02_08625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR72037.1"
                     /translation="MNNVDPYQAMALCALKTKTIRFGTAVTNMVYRDPTITACSYATL
                     NEISDGRAIVGMGTGDGPGYSLGRTATKLVDFERGLRVIRDLLHDRGIDVSKSHERSG
                     GNVKLKAGRRPVPIYISAEGPKALRVAGRTCDGVILGTGFDKQVTDWARQRVAEGAKQ
                     TGRELTDIDIMPAGMIVVDDNGDVARQRVRSRMANRAHHNFRFTMETVPEGEAAGIQR
                     FMENFNIANPIEERVDPVFVTDYLLERFTIAGTPAECIAKVKRLEAEGVKRILLTPPN
                     AIYDQVMEKWGKEVIQKF"
BASE COUNT         1321 a         1736 c         1878 g         1489 t
ORIGIN      
        1 gacaactgcc ggcgcggtct ggcgcgtcac tgtcccgatc gccgggtaat gggcatcgtc
       61 aatgctgacg gcgcgtttgc cgaatatgtc gcggtgccgg tcgcgaatct tcacagagtt
      121 ccggattcca tcaccgacga agacgcggtg ttcaccgaac cgctagccgc ggcctttgag
      181 atattgacgc agattcaaat caacccgggt gacgacattt tggttctcgg cgacggcaag
      241 ctcggcaatc tctgcgctca agtgttgcgc ctaaccggcg cgaaagtcac ggcgctaggt
      301 aagcatgccg acaagctcgc ggcgattaaa cgggcgggcg tcagaactgt tcatttgaat
      361 gactggaagc cgcggctctt cgatatcgtc gtcgaagcta gcggatcgtc atcgggttta
      421 gcactggctc tcagcgccgt gcggccgcgc gggacgttag ttttgaaaag caccattgcc
      481 ggcaagcacg aagtctcgct ggcaccgatc gttattcacg agatcaatgt catcggctcg
      541 cgttgcggcc cgtttcccga cgccctcgac gctttggcga tgaagcgcgt agcggtggcg
      601 ccgctgatcg aggcgattta ttccttgcag gatggtgtcg atgccgtcgt gcgcgcgggg
      661 gaagcagggg ctaagaaagt tttgttacgg ccataaaatt ttatcggctt ccacggaggt
      721 aaatatgaag tggggtgaac agcgttggcc gcagatgaaa gcgctcgacc gttccaaggt
      781 cgtgacgatt ttgccgactg gttccatgga acagcatgga cctcacctgc ctttgcaagt
      841 ggatcatttc atcgccaatc gcatcgccga ggatcttgaa cagcgcatgc cggagatact
      901 caccttgccg ccggtgtggg cggggacttc gggccatcac atggactttc cgggcacgat
      961 tacgctccgc ccgcgcgtgt tcatcggttt gctgcgtgat atctgtacct gcatgcatgc
     1021 tcatggcttt cgccggatga ttttgctcaa cggccacggc ggcaaccgcg ccagcttgca
     1081 ggtattaggg caagagctgt atgcggagat ggggttttcg atcaacgtga tcgtttactg
     1141 ggacctagtt cccgagctcg tcaagtcgct gaagaaatcc aagtcaaaag gcatgggcca
     1201 ctcgggcgaa ttggaaacct cgctcatgct atacctggca ccgcatttgg tcgatttgag
     1261 cgccgtgcct gacggctttc gttctgatcg gctgcagcac agccgattca tttccggcga
     1321 gggcatcacc cgctacgtca atatcaagga gcactccgac atcggtgtcg atggttttcc
     1381 cgccgcagcg agcgccaagg atggagagct gttctatcaa accgttttga ccgagatggg
     1441 aaaagtcata cgcgaattgc agcaagagga ttaattaaaa tgccgcaccc ggctgggaaa
     1501 gaaaatacaa ctcctccggt gtcgatggcc ggccaagaat tttattacgg tgtgggaagc
     1561 ggccaaagcg cgcgataatg tcgcggtgca aaatcgcgaa gtttagatag tgctcgaata
     1621 tctcccgctg gtccacatcg acgattggta ctagctcgcc caacgcactt accgaccgct
     1681 cctggtcggc cattcgttct gagtgctcca gaggcagata aaagaacact cgttcgatgg
     1741 gcctcaacaa tcggtcgacg ccgcgtgcaa cgccgtcgag agtccactcc agcgccttgg
     1801 cgtcgaaggc aaacgcccgc ggcgaatcgc ggtaaatgct gcggggaaac tgatcggtca
     1861 aaatgatcat agctaaccgc ccgcgtggtt cgaccagcca agcgtcgagc ttgccttctg
     1921 cagcgagttg cacgttcacc gcgaatcgct ggcgaatttc tcggtcggtt tcggcgttct
     1981 tcgaccacca cagatccgcg cggtccttgg cgagtgctgc actatcaggg ttcgtgccga
     2041 accagtaagt cagaatgcta tcgacgcttt ccatatcagt cacgacccgt acggcgagtg
     2101 tttattgaca agtccctcga cgaattcttt tgcttgcgcg agatcgtagc cgtagaggcg
     2161 tcgggcggtg gcgatggcgc caattttatc gccggattcc gctagatcga gcagccggag
     2221 ttcttgttca gcgcggctga actcatccag tttgagcaaa tcctttgagg ttttttcagc
     2281 ggcgcagatc ggtgtatgac ggctgagtgc attcaagaac gtcgccgcgc taggtacaac
     2341 cgcccattcg atctgcagct tatcaccagt tggcaaacgg accggctgat gctgatagcg
     2401 ggtgctgaca gccagctgcg ctttgcgctc catggcatag cggcgctcgc gttggagcgc
     2461 atctctcaag ggtttggtct taccggcgag ttcaagctca acgagcttgt tagttttggt
     2521 gctggttgcc ggccgcttat tgccgtcgcg gtccggcact tgttgcttct cgacgacata
     2581 gcgcgccgag cggatttccg catgcgcaat gaaaacaacg ataagttcat gttcgggaaa
     2641 gtggttgttc aaatacgagc gaaatttgat gaacagacca tcgtcgctca atcgcaccag
     2701 ccagttggcc ggatgaaagc gcgcaatgag caattttcgg aacaggaaga tgaagatgaa
     2761 gacgacagcg aaaataaaat aacccacctc aggttgcttc agccaagcga ggtagctgag
     2821 cgcgccggca ccggctgctg tcagcgaaat ggcccaaagg gcatgagcgc gtgaataact
     2881 aaagacctgg tcgcgttcag agattggcac ttcggccggg cacaggagct tcataaatga
     2941 tcgctcgcga acggcgattg ttggatgctc agccgtcaat gaccgagcag cgatttgagc
     3001 tgagtaaaga attcaccgat ggagccatta tgaaggaagt cggcgagatc gtcgagcggt
     3061 gtgggatcac ggttgacgat ggcgagcagc gcgccgctgc gcttggcgat cacggggaag
     3121 gatgccgcgg gttgcacttg caacgacgag ccgatgacga tgaaaatttc tgcgccttcg
     3181 ctccagcgct gagctcgttg catggcttcc accggcatcg attgtccgaa ggaaatattg
     3241 gctgatttca aaaaaccgtt acagacatcg cacgttggcg tcggcagtgc ttcgtgccat
     3301 gtttcataaa cgtcggacgg ttgaaaagct ttgccacatt tgaggcaagt gacgagccga
     3361 tcggtgccat gcagctcgac cagccgtgtg tccgatgtgc cggcgagctt gtgcaggcca
     3421 tcgatgttct gtgtaatcag accgagcagt tggccgcgct gttcgaattc gcagatctcc
     3481 cggtgcccga cgttaggtgt gacggtcttg aagagctcat aggttgcttt cttcaactgc
     3541 caatgttgta aacgcgcagc ttcgctggcg agaaaatctt ggaacagcac cggctggtat
     3601 tttgtccaga cgccgccggg gctgcggaaa tcgggtacgc cggactccgt gctaatgccg
     3661 gcgccggtga agaaaaccag ctttaccgac ttacttacca atccggcgaa cgcttcaaac
     3721 tccacagcgc aatgctctcg ggttatttct tcagcggctc ggctggcttt gccggagtcg
     3781 ctgccttact gggcggctcc gtcgcttttg cggctggcgc ggtcgacttg gcagctggag
     3841 ccgtgggcgg cggcgccggc tggatatcga cgactttgac gttgaagacg agagttttgc
     3901 ccgccatcgg atgattcata tcgatgacca cggttttttc ttttatttcc ttgatgcgag
     3961 cgaccatggg gcgtccttgg tcgtcttgtc cttgcagtac cgcaccgacc ttgagagcat
     4021 tcggcggaat ggtttcttta ggcatttcct gaaaaccatt tgggttgatt tgcccataag
     4081 cgtcgtccgg cttgaccgtg acattttttt caccgccgac tttcatcccg gtaagctctc
     4141 tctcgagtcc gggaatcatc gtcttctgcc catggacgta tttgagcggc tcccggcctt
     4201 tactactgtc gatcaccttg ccgtcggtgc ccttgagtgt gaagtctatg ctgacgagca
     4261 tgccgtcttt gacgacgttt tcattcttgg ctttttccgc cgcagctgcc gtctgcgcaa
     4321 tggcgaatga taacagcgcc gcagcaatgg cgcggaaaat aaaatttaac tgcatggagt
     4381 tctcctcttg ggtgattact gcgaagtgac aattcgtaaa gagtacatcc tggggcgagt
     4441 gaactcaata aaatcgtctc aattgggcgc gaagtttaag aacaattcac tttgtcgaag
     4501 aaagcgcggc gccgattcta cttagcgatt cgatcaaaga aaccttcacg ctgcagctca
     4561 tcgagcaaac tcatatcgat gaagcgcgcc atctccgctt cgtttttgcc gagtgtcgcg
     4621 atgagattgc gccagccggt aatttcggtg cgcgggacgc tggcgaaact gtcggcgtaa
     4681 aactttcggg tttcttccag cgcagccgca tcggtgacgc cagtgtaacg cgccagcgcg
     4741 cgataggcgg agtcgggatt acgtttgatg cggccgatgg cttctacgta cccttggagg
     4801 aatcgtttca gcaaaggccg ctgtttctcc agcgcggcgc gcgtgacgac aacggaagtg
     4861 tcggtcagat cggcataggg gcgcaggtct gcgagggtcg gaaagccttc tttggtggcg
     4921 atcaataatt ccggataagc gaatacggtg gcatcgacga cgccttcgcg tagcgccgcc
     4981 atgcgcgtcg gactcgggcc gagacttaag agtgagacac ggttgaaatc caagttccat
     5041 ttcttaaatg ccagctgtaa tcccagctcg ctcgaaccgc cgcgcgaggc gatggcgatc
     5101 ttcttgccga ccaagtcttt gacgtcgcgg atctctttgc gcgcgacgat acgccagttg
     5161 acctggttca tgacggtggc aatcatggtc agatcggcgc cggcgattcc ggcacgcgcg
     5221 acggtggcgg cgccggagtg agtcagcaac agctcgccgc tcaaaagcgt ctgaattatt
     5281 cgcgatccgc cggcgattag tagaatttcc atgtctaatc catacttttt atccaagcct
     5341 tcctgcttct gcacccacat cggcagatgg taacctgtgg tgccggcgaa gctcgcgcgg
     5401 agcttggtca gctgcgctgg gactggctcg gcagctaaca gcaacgcgcc gatgagcagt
     5461 aaggtcgagg atggaaaatg gaggatagaa gatcgcattt cgatagtctc cgtgtttacc
     5521 tttgcctgtt accgattgcc taaaatttct ggatgacttc tttgccccac ttctccatga
     5581 cctgatcgta gattgcgtta ggcggtgtca ggaggattcg cttgacgcct tcggcttcca
     5641 ggcgtttcac tttggcgatg cattcggcgg gcgtgccggc gatggtgaag cgctcaagca
     5701 ggtagtcggt aacgaagaca gggtcgactc gttcttcgat tggattggca atattaaaat
     5761 tttccataaa cctttggatg ccggcggctt cgccttcagg gaccgtttcc atggtgaagc
     5821 ggaaattatg atgagcgcga ttggccatcc ggctgcgcac tcgctggcgc gcgacatcgc
     5881 cgttatcgtc gacgacaatc atgcctgccg gcatgatgtc gatatcggtc aactcccggc
     5941 ctgtctgctt ggcgccctcg gcgacccgtt ggcgcgccca gtcggtcact tgcttgtcga
     6001 agccggtgcc caagatgact ccatcgcaag tacggccagc gacgcgcaaa gctttcggac
     6061 cttctgccga aatgtaaatc ggcaccggcc gcctgccggc tttgagtttg acattgccgc
     6121 ctgagcgctc atgacttttg gacacatcaa tgccgcggtc gtgtaaaaga tcgcggatca
     6181 cgcgcagtcc tctttcgaaa tcaacaagtt ttgtcgccgt tcggcccaag ctgtatcccg
     6241 gcccgtcgcc ggttcccatg ccgacgatgg cccggccgtc ggaaatttca ttgagtgtgg
     6301 cgtaagaaca cgcggtaata gttggatcgc ggtagaccat gttggtcacc gcggtaccga
     6361 agcggatggt tttggtcttc agcgcgcaca gcgccattgc ctggtatggg tcgacgttgt
     6421 tcat
//
LOCUS       DATLCH010000294         8092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7114283, whole genome shotgun sequence.
ACCESSION   DATLCH010000294 DATLCH010000000
VERSION     DATLCH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7114283"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..563
                     /locus_tag="VMZ02_08630"
     CDS             132..563
                     /locus_tag="VMZ02_08630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HUR72038.1"
                     /translation="MSEFAIHPQLLADCHRLGRFDLCHVLLNSNASLPWFILVPETAV
                     TELFDLADTPRTAVLHEAAVVAQFIKNHLGYQKINFAAIGNIVPQLHLHIVGRKLGDP
                     CWPAPVWGKIIESQEYSGQRLEGIRELLVRQCSLKKELANP"
     gene            complement(607..1194)
                     /locus_tag="VMZ02_08635"
     CDS             complement(607..1194)
                     /locus_tag="VMZ02_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HUR72039.1"
                     /translation="MNSDERSAYLRELRPYLVASIIFFAVGAVIGAAVASRFPGLADN
                     FGDSIAGFLKTFRGLPKPQLAAAIFLNNSIKTLAAILLGLAIGVVPALFLIINGVVLG
                     VVFFLSSYSRGLWPSLLSILPHGILELTAVFLGTAIGLLLGNIVLKRMLRKSDAKIRP
                     ALIRGLRFYAIVIVPMLLVAAMIEAFITTMIVGAR"
     gene            1551..2642
                     /locus_tag="VMZ02_08640"
     CDS             1551..2642
                     /locus_tag="VMZ02_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HUR72040.1"
                     /translation="MKIKSIHSQIVRLPVDEPLADGPPSGRTHNMFVTVRIETADGIE
                     GIGATFYGGVLSAALKQALDQLGALIIGEDPLRIDALTQKLRMAAASAGPGGILTLAI
                     SGIDMALWDLKGKYLGQSLGAMLGGLRDRVPAYASGALVRSYSLDHLLKSCRTLIERG
                     FKQMKTQMALPGEVTPAQEAERARLTRQAVGPDIDLMCDINQRWSVNQAIDIGRRVED
                     VHFFWLEDVTVHDDYTGLARVAAALATPLASGECLYGITPFRHTIENGAADIIMIDLI
                     RVGGVSNWMKVAAMAEAFNLPVVSHLFPEIHVHLMSSIPNGLTVEYMPWSFRLFEEVP
                     VPVQGELIVPTKPGLGLAFNRELDRYVVG"
     gene            2752..3750
                     /locus_tag="VMZ02_08645"
     CDS             2752..3750
                     /locus_tag="VMZ02_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72041.1"
                     /translation="MEKRGKGRFGLAAVWREMGIVILHVAFALDQTSAADLTKIYAGY
                     GGIAGYQMPLWVIKEADIGKKYGIDIEPLLISGGALSMQALLANSIQLSQNSASAAAG
                     AALRGAPIALVSTSENRMAAEIVARAEIKTPQQLIGKKIGILRFGGSNDTAIQWALRA
                     WKIDPKQVTILQSGATNARLLALTLGQIDATILSYPEIHIARKRGMNVLADVGDFSAY
                     PNTSFMLTRAFIEKQRPLAKNLLRSQIEAIHYMRTNKEGSIKILKRYLRVDDPEATDA
                     TYEFFARRTDPLPRTNLEGLKNILREMNAPQRSPAEFVDTSLLDEIEKEGFMQRFR"
     gene            3882..4292
                     /locus_tag="VMZ02_08650"
     CDS             3882..4292
                     /locus_tag="VMZ02_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HUR72042.1"
                     /translation="MDAVRSVKAIAGEGILGNADRGGSRQITLLEKEVWDALMQSLDG
                     AAPASARRANLLVSGIALANSRGKILRIGSVRLQIGGETKPCERMDEVLPGLETAMYT
                     EWRGGAYARVLSGGEIAVGAKVEWEEPAIGGLQG"
     gene            4305..4694
                     /locus_tag="VMZ02_08655"
     CDS             4305..4694
                     /locus_tag="VMZ02_08655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUR72043.1"
                     /translation="MRIKSGIKLLAEVEGQGEPAERGDSVVFNWRLYRNHGEEISVNE
                     QQAENLPREMIRHMDGRPLVDHKTTLGKRQTTAGVEYSLYGMKTGGYRKIRVSPHLGY
                     RDEGLGALIPPHAVLIIEIWLREISRR"
     gene            4780..5175
                     /locus_tag="VMZ02_08660"
     CDS             4780..5175
                     /locus_tag="VMZ02_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HUR72044.1"
                     /translation="MKSGKQKRSAIKANRKQKSGKFPGSANRPAPQQRPVLGPPVNEA
                     LLAPNNSYGAPDFVSRGYYVDRPFRCIDCGKEEIWTGTQQKWWYEIAKGFAYTTAVRC
                     RLCRRKKREQSEKSRRIHLEGLERKRDTK"
     gene            5357..5839
                     /locus_tag="VMZ02_08665"
     CDS             5357..5839
                     /locus_tag="VMZ02_08665"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HUR72045.1"
                     /translation="MGLLDGLLGNVLGGALGGNRTQSQAQDPLSSILGGLTGGRGGGT
                     GNILLQLALSMLQQQGGLGNVLGKFREAGMGAQADSWVGTGQNVNISPNQLEQVFGSG
                     ALNDIASKLGMSQEQAGSAMSQVMPELINQLTPQGQVTADSENSVAEGLDALSASLDR
                     "
     gene            5876..6424
                     /locus_tag="VMZ02_08670"
     CDS             5876..6424
                     /locus_tag="VMZ02_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72046.1"
                     /translation="MAGDSVFLALNNIDKFQLWSMLFLTLARIDSSAAAEIDFVTLAN
                     LPPVVRESFENSPQAKGYAFSAHINPFYLHGDFDGDGQLDTAVWITERATGKSGIAIF
                     HGKANRLVIVGAGRELGSGSDDFSWLNAWHVFRRGPVERGADNKPPPKLRGDALVVIK
                     TESASALIYWNGKRYGWYQQGD"
     gene            6426..6938
                     /locus_tag="VMZ02_08675"
     CDS             6426..6938
                     /locus_tag="VMZ02_08675"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HUR72047.1"
                     /translation="MRRDDGAGAWVSLIFLAVFALVFVVYTTRYLPDPVATHFGGDHR
                     ANGWMSREGYLLFMLAFMVGISALVSFTIGTLPGKFPQWTNVPNREYWLAPARREDSL
                     RYLSAHGKRLGCLIIMMMLGMHYVILKANFVRPPVLPVPTFSAVLFGFALMLIWWIVR
                     LYRRFPKPKG"
     gene            7035..7643
                     /locus_tag="VMZ02_08680"
     CDS             7035..7643
                     /locus_tag="VMZ02_08680"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HUR72048.1"
                     /translation="MKGFDNNNRCPWVDTTKPDYVEYHDLEWGVPVHDDRVIFEFLTL
                     EAAQAGLSWYTVLRKRKSYRDAFADFDPQKVARYGKKQIQALLKNPGIIRNRLKITAA
                     VNNAGRFLQVQDEFGSFDAYVWRFVDGKPIVNRFKSLKDYPATSRESDALSKDLKQRG
                     FKFVGSTIIYAHMQATGMVNDHSEACFRRKPIIAEHGRLTTR"
BASE COUNT         1813 a         2112 c         2347 g         1820 t
ORIGIN      
        1 ggctggtacg accccgcgga tcggctcgtc agcaccaaac gaatgcacgc aaaccctgaa
       61 cagcgcgacg acagtattgg ctttcgctgc gccagcgacg ccaaataatc ctgctgccag
      121 gcggatcggc aatgtctgaa tttgcaatcc atcctcagct gctcgccgat tgccatcggc
      181 ttggacgatt cgatctatgt cacgtgttgt tgaacagcaa tgcgtcgctt ccgtggttta
      241 tcctagttcc agaaaccgct gtcactgaac tgttcgatct tgcggacacg ccgcgaacag
      301 ccgtactgca cgaagccgca gtcgttgcgc agttcattaa aaatcatctc ggctatcaaa
      361 agatcaactt cgccgccatc ggtaacatcg tgccgcagct gcacttgcat atcgtcggcc
      421 gaaagctcgg cgatccgtgc tggccggcgc cggtgtgggg caaaattatc gaatcgcaag
      481 agtattcggg tcaacgtctc gaagggattc gcgagctgct ggtgcgccag tgttctctta
      541 agaaagaact cgccaatccg tgaagtgctg aagcgcttcg gcgtgacacc gatgctgctg
      601 cgaaatttac cgcgctccga cgatcatggt tgtgatgaag gcttcgatca tagcggcgac
      661 cagtaacatg ggcacaatga caattgcgta aaatcgcagg ccgcggatca atgccggtct
      721 aatcttggcg tcggattttc ttaacattcg ttttagcacg atgttgccca aaaggaggcc
      781 gatcgcggtg ccgagaaaca ctgcagtgag ttcgaggatg ccatgcggca agatgctcaa
      841 aagcgatggc caaagtccac ggctataaga ggaaagaaag aacaccaccc cgaggacgac
      901 gccattgatg attagaaata atgccggcac gacgccaatc gccaggccta acagaatcgc
      961 ggcgagagtt tttatcgagt tgttgagaaa aattgccgcg gcaagctgtg gtttcggcaa
     1021 gccccggaag gttttcagaa agccggcgat ggagtcgccg aaattgtccg cgaggccggg
     1081 gaaacggctc gcgaccgcag cgccgatcac tgcgccgacg gcgaaaaaaa tgatgctagc
     1141 gacaagatac ggacgcagtt cgcgcaaata tgcgcttcgt tcatcgctgt tcatcaaact
     1201 atttttagca gaaagcggct gcggtagata tgccccggag aaatggatcg gagcgcgccg
     1261 caaattgcct gatgcaagga ttgtctgacc ggagattgtc gacgatgcta cgtcttctgc
     1321 tacaggtagt tttggtattg ctcgtctgtg tccggttaag tctcgtcgtt acttttatgt
     1381 tgctgcccgt tcggtcttga ttcgaagcga gttacaaaat cggtggggca ttctggacca
     1441 gccgagtagt gctatgttct cgtgtacggc tttttgaatc ttctagtctt tgcggtgctg
     1501 tggtatcgct ggaacgccgc caacagagca caagctgtaa agggattacg atgaaaatca
     1561 aaagcattca ttcacaaatc gtccgtctac cggtcgacga gccattggcc gatggcccgc
     1621 cgtcgggaag gacacacaac atgttcgtca cggtccggat cgaaacagcg gacggtatcg
     1681 aaggcatcgg cgcgacgttc tatggcggag tgctcagcgc ggcgctcaag caagctctcg
     1741 atcagctcgg cgcgctgatc atcggagagg atccgctgcg gatcgatgcg ctgacacaga
     1801 aactgcgcat ggcggcggcg agtgccgggc caggcggaat cctgacactg gcgatttccg
     1861 gtatcgatat ggcgctgtgg gatctcaagg gcaagtatct cggtcaatcc ctcggcgcta
     1921 tgctcggcgg cttgcgtgac cgtgtgccgg cctatgccag cggcgcgctg gtacgcagct
     1981 attcgctcga tcatctgctc aagtcctgtc gcacattgat tgaacgcggc tttaagcaaa
     2041 tgaaaaccca gatggcgttg ccaggggaag tgacgccagc gcaggaggcc gaacgcgcgc
     2101 gcttgactcg ccaagcggtt ggccccgata tcgatctcat gtgcgatatc aatcaacgct
     2161 ggtccgtcaa tcaggcgatc gacattggcc ggcgcgtaga ggatgtgcat tttttctggc
     2221 tcgaagatgt caccgtccat gacgattaca ccggcttggc ccgcgtcgcg gccgcattag
     2281 ccacaccgct tgccagcggc gagtgcctgt acggcattac gccgtttcgc cataccatcg
     2341 aaaatggcgc cgccgacatc atcatgatcg atctgattcg cgtcggcggt gtttccaatt
     2401 ggatgaaagt cgcggccatg gccgaggcgt tcaatttgcc ggtcgtgagc catttgttcc
     2461 cggagattca cgtgcacctg atgtcgtcga ttcccaatgg cttgactgta gaatacatgc
     2521 cctggtcgtt tcgtttgttc gaagaagtgc ccgtgccggt gcaaggtgag ttgatcgtac
     2581 cgaccaaacc ggggttgggt ttggcgttca accgggagtt ggaccgatac gtcgtggggt
     2641 aattcctttc aacgaggtca gggtgaatat ttttactttg caattaataa aatccccctc
     2701 gatccccctt gtcaaagggg gaaattgcgg gctcggggaa aattcccctc tttggaaaag
     2761 aggggtaagg ggagatttgg gcttgctgcg gtgtggcgcg agatgggtat cgttatactg
     2821 catgtcgcct ttgccctcga tcagacttca gccgcggatc tgaccaagat ttacgctggc
     2881 tacggcggca ttgccggtta ccagatgccg ctgtgggtga ttaaagaggc ggatatcgga
     2941 aaaaaatacg gcatcgatat cgagccgctg ttgatcagcg gcggcgccct aagcatgcag
     3001 gcgctgctcg ccaacagcat tcagctgagc caaaactcgg cctccgcagc ggccggggcg
     3061 gcgttgcgcg gtgcgccgat cgcgctcgtc tcgacatcgg aaaaccgcat ggcggctgag
     3121 atcgtcgcgc gcgccgaaat caaaacgccg cagcaattaa tcggcaagaa gatcggcatt
     3181 cttcgtttcg gcggctccaa cgacaccgcc attcaatggg cgttacgggc gtggaaaatc
     3241 gatccgaagc aggtgacgat tctccagtcc ggcgcgacca acgcccgtct actcgcattg
     3301 acgttggggc agatcgacgc gacgatactt tcctatccgg aaattcatat cgcccgtaaa
     3361 cgcggcatga acgtattggc cgatgtcggc gatttcagcg cttatccgaa cacttcgttt
     3421 atgctgacgc gcgccttcat agaaaagcag cggccgctgg cgaaaaacct gctccgttcg
     3481 cagatcgaag cgatccacta catgcgcacc aacaaggagg gcagtatcaa gatcctcaaa
     3541 aggtatcttc gcgttgacga tcctgaagcc accgatgcga cctacgaatt cttcgcccgc
     3601 cgcaccgatc ctttgccgcg gaccaatttg gaaggattga aaaacatcct cagagaaatg
     3661 aacgcgccgc agcgtagtcc cgccgagttt gtcgatacca gcctgctcga cgagatcgag
     3721 aaggaagggt tcatgcagcg gttccgttaa aaaacaccct gatgaagaat aacttgagat
     3781 tatctaaggt gacacttcaa cccacttctg cagggacaat ctcaattatg cgcgacggcc
     3841 gtgtcgagac gatctggata aaacgggccc atcgcgggcc aatggacgcg gtcagatccg
     3901 tcaaagctat cgcaggcgaa ggaattctcg gcaacgccga tcgcggcggc tcgcgccaga
     3961 tcacgttgct ggaaaaagaa gtttgggatg cgttgatgca aagtctcgac ggtgcggcgc
     4021 cggccagcgc gcgccgggcg aacttgttgg tgagcggcat tgcactggcc aattctcgcg
     4081 gcaaaattct tcgcatcggc tcggtaaggt tgcagatcgg cggtgagacc aagccttgcg
     4141 aacggatgga tgaagttttg ccgggactgg agacggccat gtacaccgaa tggcgcggcg
     4201 gcgcctatgc gcgagttctc tccggaggtg aaatcgcggt tggcgctaaa gtggagtggg
     4261 aagagccggc tatcgggggc ctccagggtt aagctattca tgcgatgaga attaagtccg
     4321 gcatcaaact actggcggag gtcgaaggac agggcgagcc ggccgagaga ggcgatagtg
     4381 ttgttttcaa ttggcgactc tatcgcaatc acggcgagga gatttcggtc aacgagcagc
     4441 aggcggaaaa tcttcctcga gaaatgatcc gccatatgga tggccggccg ttagttgatc
     4501 acaaaacaac cttgggaaag cgccagacga cggctggcgt ggaatattcg ctctatggca
     4561 tgaagaccgg cggctatcgc aagattcgtg tcagcccgca cctcggctat cgggacgagg
     4621 gactgggcgc gctgataccc ccacacgccg tcttgatcat cgaaatatgg ttacgagaaa
     4681 ttagccgccg ttaagctagg gccggcttat ttgcgggtac atccactcag ccaccggttt
     4741 tgttagcgct gagaacagta tctaacccgc caagtgaaaa tgaaaagcgg caaacaaaag
     4801 cgctccgcga ttaaagccaa ccgaaaacag aagagcggca agtttcccgg gagcgccaat
     4861 cggccggcgc cgcaacagcg tccagtgctc ggaccgccgg tgaatgaggc tttgttggcg
     4921 ccgaacaata gttacggtgc gccggatttt gtctctcgcg gttattacgt cgaccgtcca
     4981 tttcgctgta tcgattgcgg caaggaagaa atatggaccg gcacgcagca aaaatggtgg
     5041 tatgaaattg ccaaggggtt tgcttacacg acggcggttc gctgccgttt gtgccggcgc
     5101 aagaagcgcg agcagagtga aaagtcgcgc cgcatccatt tagaagggct cgaacgaaaa
     5161 cgcgacacaa agtgaaagaa tcgctttaat gatagcgccg cgacgttccg ccgcgtcgtc
     5221 catctgcaag gcgcgaaagt aacaaagggt ggagaaggct gaatgtctcg gcgaagggcc
     5281 agccatcttg tagccatatt ctcaactgta ttagtatccg gctgcttgat cattattcgg
     5341 cgaaaaggag agtcgtatgg gattattaga tggcctattg ggtaacgtgc ttggcggcgc
     5401 attgggcggt aaccggacgc agtcacaagc gcaagatccg ttgagctcaa ttctcggcgg
     5461 gctcactggc ggccgcggcg gcggcacggg aaatatttta ttgcagctcg ctctttcgat
     5521 gcttcagcaa cagggcgggc tcggcaatgt gcttggcaag tttcgcgaag ccggcatggg
     5581 cgcgcaagcg gactcctggg tcggcaccgg ccagaatgta aacatctcgc cgaatcagtt
     5641 ggagcaggta tttggttccg gtgccttgaa cgatatcgcc tcgaagctcg gcatgtcgca
     5701 agaacaagcg ggctcagcga tgtcgcaggt gatgcctgag ttgatcaatc agctgacacc
     5761 gcagggacaa gtgactgcgg attcggaaaa ttcggtagcc gaaggcctgg atgcgttgtc
     5821 tgccagcctg gaccgataaa tttttcgcaa gaaatttcag gatcgctggt cgtctttggc
     5881 cggcgattcg gttttcctcg ccttgaataa tatcgataaa ttccaactct ggtcgatgtt
     5941 gtttttgacg ctggcgcgaa tcgattcgag cgccgccgct gagatcgatt ttgtcactct
     6001 ggcaaatttg cctccggttg tgcgagagtc attcgagaac agtccgcaag ccaagggcta
     6061 cgccttttcg gcgcatatca atccgtttta tcttcacggt gatttcgatg gcgacggcca
     6121 gctggacacg gcagtttgga tcacagagcg cgcaaccgga aaaagcggca tcgcgatttt
     6181 tcacggtaag gccaatcgtc ttgtcatcgt cggcgccggc cgcgaactgg gcagtggcag
     6241 cgacgacttt tcttggttga atgcctggca tgtttttcgc cgcggcccgg tggagcgggg
     6301 cgccgataac aaaccgccgc caaagctccg cggcgatgcg ctcgtggtca tcaaaaccga
     6361 atccgccagc gcattgatct attggaatgg caaacggtat ggctggtatc agcagggaga
     6421 ctagcgtgcg gcgtgacgat ggtgcgggtg cctgggtgag cttgatcttc ctggcggtgt
     6481 ttgccctcgt gtttgtagtt tacacgacac gctatttgcc ggatccggtg gcgactcatt
     6541 ttggcggcga tcaccgggcc aacggttgga tgagccgtga gggctatctc ctgttcatgc
     6601 tggcgtttat ggtcggtatc tccgcgttgg tcagcttcac gatcggaacg ttgcccggta
     6661 aatttcctca gtggaccaat gtgcccaacc gcgagtactg gctggctccc gcgcgccgtg
     6721 aagactcact gcgctacttg tcggctcacg gcaagcgcct cggctgtctg atcatcatga
     6781 tgatgctcgg catgcattac gtgatcttga aagctaactt tgtacgaccg ccggtgctgc
     6841 cggtgccgac cttttcggcg gttcttttcg gcttcgccct aatgctcatc tggtggatcg
     6901 tgcggctgta ccggcggttt ccgaaaccga agggctgaga tgaaccacga aatgtacgaa
     6961 atacacgaaa atctcagcca taaggtattc taaatttagt gcctttcgtg tttttcgtgg
     7021 ttaatattct ttttatgaaa ggctttgata ataacaatcg ctgtccgtgg gtggatacga
     7081 ccaagccgga ctatgtggag tatcacgatc tcgagtgggg cgtgccggtg catgacgacc
     7141 gcgtgatttt cgagttcctt acgcttgaag ccgcgcaggc ggggctgagc tggtatacgg
     7201 tgctgcgcaa acgcaaatct taccgcgacg cttttgccga tttcgatccg caaaaggtcg
     7261 cgcgctacgg caagaagcag atccaggcgt tactgaaaaa ccccggcatt attcgcaacc
     7321 gattgaaaat caccgccgcc gtcaataacg ccggtaggtt tttacaagtt caagacgagt
     7381 tcggcagctt cgatgcttat gtttggcgct tcgtcgacgg caaaccgatc gtcaatcgct
     7441 ttaagtcact gaaagattat ccggccacca gccgtgagtc cgatgctttg agcaaggatt
     7501 tgaaacagcg cggtttcaag tttgtcggtt cgacgatcat ctatgcccac atgcaggcca
     7561 ccggaatggt caacgaccac tcggaagctt gtttccgccg taagccgatc atcgccgagc
     7621 atggtcgatt gacgactcga tgaaattcct ctggctggcg tgtgccttag cctgcacgtt
     7681 ttcgtttacg acgtggggtg aacggtaggt ccattccgac ggcatttaaa attgcggcga
     7741 gagatgaaaa ctttggattt cctttttcag agaggactcg ataaagattc tctcgattaa
     7801 gcgtagttag tttcgaaagt gctcgaactc ctcctcctgc ctcaacgacg ttacgaagac
     7861 cgacgaagaa aatctcttcc gagtttgatg gcaagcttga gatactctgc ggcttcgttc
     7921 gggtcgcgca attgtttgag caactgactc taatgacttc ttgttggcat gatactccct
     7981 ccaaagattt ttggccttaa cgatatcgcg tttttgtgaa cccttgtcgc tgccgcagtg
     8041 acaacgcgac tattctgctg tcatttcgag cgaagcgaga aatctttctc ag
//
LOCUS       DATLCH010000295         5017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7119373, whole genome shotgun sequence.
ACCESSION   DATLCH010000295 DATLCH010000000
VERSION     DATLCH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7119373"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VMZ02_08685"
     CDS             <1..417
                     /locus_tag="VMZ02_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72049.1"
                     /translation="FIGTSAGGSAAIRIGHDLGADYVHAFGAQTGLSSIAGEKGEREP
                     AMELAPLLKNANRKTLYYLHYCLGYEIDRLHAERVAGAPGVITLGYPGAIHRVITPLA
                     QKGLLAKLLLIDNQKHIVAIAREFYGDEVKVTGASS"
     gene            461..1957
                     /locus_tag="VMZ02_08690"
     CDS             461..1957
                     /locus_tag="VMZ02_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HUR72050.1"
                     /translation="MLFDVLSETAHAHPDKIAVAGERRSLTYSQLHHEASCFALYLEE
                     LGFKSGESIFIGLPSSPDFFVAIYGAWALGLLVVPVSPTGPIASTYLATNPVGAIGTP
                     DFLKQIKAQCRGLRTVIPCSSSGGFAVTDFPGELKSRRPFKQEAVLCVSSSGTTGEPK
                     LYQRSCELIHGHARTRVEVQQINSSDIFLSMRPLNNANAINNFVVPPIMAGAKVILHE
                     RFERFKAVDAIRAERVTVLNSVPFVFELLASIPESRPVDFSSIRMCIAGGAPLPPSVA
                     DQFFRRFGMRIHQRYAGSHFYPAFSFNFDGPSDSVGRIDGYFPMVILDEDGKRLEPGR
                     IGEIAFTYANLPPTLQRVAESNPNRQNDCILTGDLGRTDADGNVFVVGRRSPFIKIGG
                     NRVEPAEVESTLRSHPAVREALVYPLRQGQTDEAIGAMVAASNVSEQELLQHCADHLE
                     AFKCPRQIDVKDELPRTEQGKISRRLFESLQWLILILEGANNCANIYV"
     gene            2078..2359
                     /locus_tag="VMZ02_08695"
     CDS             2078..2359
                     /locus_tag="VMZ02_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HUR72051.1"
                     /translation="MARKNITTRLKAVLRDVVGFSEASLRRDNFTLFGSRGRIDSVGV
                     LMLVMALEKQFGVIIDDGEILPKNFQTVKKLAAFIERKLSSPGVAQRNL"
     gene            complement(2493..3311)
                     /locus_tag="VMZ02_08700"
     CDS             complement(2493..3311)
                     /locus_tag="VMZ02_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR72052.1"
                     /translation="MAQGCATQIARRSLTTSDGVVLSVLESGREHAAGDKIQIALVTG
                     WSMPASIWQAQLDEFGRSYHTLAIDPRGQGESQVPADGFTAERRASDLKELLEPLKNV
                     LLVGWSLGALESLQYVHMFGAGRLVGMVLVDSSVGEEPEPAPGGTFLQALRNDRDKTL
                     DGFIRAIFQNQRSDAEVAALTNGAKRMSVDQSIALLSYPFPRTHWKEIAHAFPKPLLY
                     VVTPQFAEQAANLQKHRPGTEVELFKDARHALFVDEPERFNALILKFAKSLSPQ"
     gene            complement(3349..4494)
                     /locus_tag="VMZ02_08705"
     CDS             complement(3349..4494)
                     /locus_tag="VMZ02_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72053.1"
                     /translation="MAKRKILRWVILSALLSGNSPVAAVEAPNPEFNRRATATAQMLA
                     GITPNPPEPAFNRLLELDAWKEHQKWMTSQWAEVRGRIKTVEGWREHELKIASPQKKT
                     LLYPFSGPDFFNAYAFFPDHSRYVFFSLERPGALPDLESVTTQQFAKLLTDVRGAFRD
                     IFERNYFITSYMNKQLTTPWVRGTVPVMATMMALMNRRIVRIEPIDLFPELTKAYDTP
                     DGKRPRLLLRGVRIEFSNPSTPGVQQLYYYSLDATDKALEFYPEFLNWVGQNRPASAL
                     LKSASYLLHDGQFSKTRAMVLDSADVLIQDDTGIPYRYISQSPWQVKLYGKYHKPIKP
                     MAYGYQKDLDAAFKAKVENTELPFPFGYHWRGSQGGLILANRERNSN"
     gene            4775..>5017
                     /locus_tag="VMZ02_08710"
     CDS             4775..>5017
                     /locus_tag="VMZ02_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72054.1"
                     /translation="MSGWKARIGYLSPSVFETPSDWDLILPEGFTLVSSGLNVQSHTE
                     AEFNKAINALGAGLDIFKAEECDVILMGGITLGTQRG"
BASE COUNT         1137 a         1384 c         1345 g         1151 t
ORIGIN      
        1 tttatcggca cgtcggccgg aggatccgcg gccattcgta tcggccatga cctcggcgct
       61 gattacgttc atgcgttcgg cgcacaaacc ggactctcgt cgatcgccgg cgaaaaaggc
      121 gaacgtgaac cggccatgga actcgccccc ctactcaaaa atgccaatcg aaaaacgctt
      181 tattatcttc attattgctt gggatatgaa atagatcgat tacacgcgga aagagttgca
      241 ggcgctcccg gtgtaatcac tctaggatat cccggggcca tccatcgagt tatcacgcca
      301 ctggcgcaga agggattgct ggcgaaatta cttttgatcg ataatcagaa acacatcgtt
      361 gccatcgccc gcgaatttta cggcgacgag gtcaaagtca caggcgcgag ctcataagtt
      421 ttatcgggta ttgcgagacg cggctcaaat aatctgccct atgctttttg atgtactaag
      481 cgaaaccgcg cacgcgcatc cagataaaat agctgtggcg ggagagcgta ggtcgctcac
      541 ttattctcag ttgcaccatg aggcgagctg tttcgcgctt taccttgagg agttgggatt
      601 caaaagcggc gaatcgattt ttatcggctt gccatcgtca ccagattttt tcgtcgccat
      661 ctatggcgcg tgggcattag gactgctggt cgttccagtc tcgcccaccg gaccgatcgc
      721 atcgacgtat ttggcgacca atcccgtcgg ggcgatcggc acaccagatt tcttgaagca
      781 aattaaggcg caatgccggg gcttgcgcac cgtgatcccc tgctcatcgt ctggtggatt
      841 cgccgtcacg gacttccccg gagaactcaa gagtagacga ccgtttaagc aagaggccgt
      901 cctgtgtgtt tcgagttccg ggactaccgg cgagccgaaa ctctaccagc gctcctgcga
      961 gttgattcat ggccacgcca gaactcgcgt cgaagttcaa cagatcaact ccagcgatat
     1021 ctttttatcc atgcgcccgc tcaacaacgc caatgcgatc aacaacttcg tcgtaccgcc
     1081 gattatggct ggtgccaaag ttatcctgca cgaacgcttc gaacggttta aagcagtcga
     1141 cgcaatacgt gcagaacgcg tgaccgtgct caatagcgta ccgttcgttt ttgaattatt
     1201 ggcttctata ccagaatcgc ggccagtcga tttttcgtcg attcgtatgt gcatcgccgg
     1261 cggcgcgcca ttaccgccgt ccgtcgccga ccaatttttc cgccgattcg gtatgcgcat
     1321 tcaccaacgt tatgccggca gccattttta tccggcattt tcatttaact ttgacggccc
     1381 cagcgattcc gttggacgca tcgatggtta cttcccaatg gtgatactcg acgaagacgg
     1441 caagcgcttg gagcccgggc gcataggcga aattgccttc acctatgcca atttgccacc
     1501 cacgctgcaa cgcgtcgcag agagcaatcc gaatcgccag aacgattgta tcttgaccgg
     1561 agacttgggg agaaccgatg ccgatggcaa tgtttttgtc gtcggccgcc gcagtccgtt
     1621 cattaaaatc ggcggcaatc gtgtcgaacc ggccgaagta gaaagcacct tgcgttccca
     1681 cccggcggtg cgggaagcgc tcgtctaccc gcttcgccag ggtcaaacag acgaagcgat
     1741 cggcgccatg gtcgctgcca gcaatgttag cgagcaggaa ttattgcaac attgtgccga
     1801 ccatctggaa gcgtttaagt gcccgcgcca aattgacgtt aaagacgaac tgccgcggac
     1861 cgaacaggga aaaatttcgc ggcgcctctt cgaatcgcta cagtggctca ttcttatact
     1921 agagggcgcc aacaactgcg cgaacatcta tgtctgattt gttattactt tccagcgcag
     1981 cagcgcccca gactcgattc gtatacggcc gaaacccact cccgcttagt tccctcgggt
     2041 gcttttcacc ggcctagatt tgttttgctg agtgtttatg gcgcgtaaaa atatcacgac
     2101 tagattgaaa gcagttttac gcgacgtcgt cggcttctcg gaagcgtcac tgcgccgcga
     2161 taattttacc cttttcggtt cccgcggccg catcgactcg gtcggtgtgc tgatgctcgt
     2221 gatggcactt gaaaaacagt tcggcgtcat catcgatgat ggcgaaattc ttcccaagaa
     2281 ctttcagacg gtcaaaaaat tagctgcttt tatcgagcga aagctttctt cacccggtgt
     2341 cgcgcaacga aatttataat tagagcccgc tagcccattc cattgagcct tctcgtcccg
     2401 gcgatagccg acccgcgcct tgccgagcga ttagccgccc cgcaagggca aaccagcgca
     2461 gtcgttagca agaacgctgt gagacaaaac gactattgcg gcgacagcga tttggcgaat
     2521 tttaggatca gcgcgttgaa gcgttcaggt tcatcgacaa acaaggcatg cctggcatcc
     2581 ttgaagagtt ccacctcagt gcctggccgg tgcttttgga gattggccgc ctgctcggca
     2641 aactgcggcg tgacgacgta gagcagcggc ttagggaaag cgtgtgcgat ctctttccag
     2701 tgcgtccgtg ggaacggata cgataacagc gcgatgcttt gatcgaccga catgcgttta
     2761 gcgccgttgg tcaatgcggc gacttcggcg tcgctccgtt ggttctggaa aatcgcccgg
     2821 atgaagccgt ccaaagtctt gtcccgatcg ttgcgtagcg cttggagaaa agtacccccg
     2881 ggcgcaggct ccggctcctc gccgacagaa ctgtcgacga gcaccatacc caccaatcgc
     2941 ccggcgccga acatatgtac atactgcagc gactcaaggg cgccgagtga ccaaccgacc
     3001 agaaggacgt tcttcagagg ctcaagcagt tctttaagat cactggcgcg gcgttcagct
     3061 gtgaagccat cggcgggaac ttgcgattca ccctgaccgc gcgggtcgat ggcaagcgta
     3121 tgatagctgc ggccgaactc gtccagttga gcttgccaga tgctcgccgg catcgaccag
     3181 ccggtgacta aggcgatctg aattttgtcg ccggcagcgt gctcgcggcc cgattcgagg
     3241 acggaaagca caacgccatc gctggtcgtg agcgagcggc gtgcgatctg agtggcgcag
     3301 ccttgtgcca tgaggacaag acagctcacg agcagcaaag ctcgtggact aatttgaatt
     3361 gcgttcccga ttggcgagta tgagaccacc ctggctgccg cgccagtgat atccgaaggg
     3421 aaacggcaac tccgtgttct caactttcgc tttgaaggcc gcgtcgagat ccttttgata
     3481 accatacgcc attggcttga ttggcttgtg gtacttaccg tagagcttta cctgccaagg
     3541 agactggctg atgtatcgat atggaatccc cgtatcgtct tgtatgagaa catcggcgga
     3601 gtccaaaacc atcgcccgcg tcttggaaaa ctggccgtcg tgcaagaggt aagacgccga
     3661 ctttagcagc gcactggcgg gccggttctg acccacccaa ttgagaaatt ctgggtagaa
     3721 ttccaaagct ttgtccgtgg catccagaga atagtagtaa agctgttgca cgccgggcgt
     3781 gctcggattg gagaactcga tacgcacgcc gcgcaggagc aaacgcggtc gtttgccgtc
     3841 cggcgtatca taggccttgg tcaattccgg gaacagatcg atgggttcga tacgcacgat
     3901 ccgccggttc atcagcgcca tcatagtcgc cattaccggc accgtgccgc gcacccacgg
     3961 ggtggtgagc tgcttgttca tataggacgt tatgaaataa ttgcgctcga atatgtcgcg
     4021 aaaggcgccg cgcacgtcgg ttaacagctt ggcaaattgt tgcgttgtta ccgactctag
     4081 atcaggcaag gcacctggac gctcgaggct gaagaaaaca tagcgggaat gatccggaaa
     4141 aaacgcataa gcgttgaaga agtccggccc gctgaaggga tagagcaagg tctttttttg
     4201 gggactagcg attttaagct cgtgctcgcg ccatccttcg acggttttga ttcgtccgcg
     4261 cacctccgcc cattgcgaag tcatccactt ttgatgctcc ttccaggcat cgagctcgag
     4321 caaacggttg aatgccggct caggcgggtt cggcgtgata ccggccagca tctgcgccgt
     4381 ggcagtggcg cgtcgattaa actccggatt cggtgcctcg accgccgcaa ccggactatt
     4441 gccggacaag agcgcgctga gaatgaccca tcgtaaaatt tttcttttgg ccaaaacctg
     4501 acaaacgttc cttttaagat tcatacttgt ttaccaaata ccgatcgaac ctttctgttc
     4561 gaggagccga tcagggaaca cgaataatgt ctgtagatta gacgctaaga atagcatttt
     4621 caccccgcca cctgtcaaat gttccgaaga tttatcgcac gtcgagcgtg aattaaacga
     4681 ttcgacgctc gatatcagcg tacgtgcacc gcttcctatc catccgtgct tacgataagt
     4741 aagatttcaa tcgtcattat aggggagaaa ccaagtgtcg ggctggaaag cacgcatcgg
     4801 ctatttatcg ccatcagtat ttgaaacgcc atcggactgg gatctcatcc ttccggaagg
     4861 cttcaccctg gtctcctccg gtttaaacgt tcaatctcac accgaagcgg agttcaacaa
     4921 ggcgatcaat gctctaggcg ccggccttga tatttttaag gcagaggaat gcgacgtgat
     4981 cctgatgggc ggaatcacgc tcggtaccca acgtggc
//
LOCUS       DATLCH010000296         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7119476, whole genome shotgun sequence.
ACCESSION   DATLCH010000296 DATLCH010000000
VERSION     DATLCH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7119476"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="queG"
                     /locus_tag="VMZ02_08715"
     CDS             complement(<1..691)
                     /gene="queG"
                     /locus_tag="VMZ02_08715"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HUR72055.1"
                     /translation="MEASSYPAREFLSAKIKDAAQELGFDLVGISPVQLPPHGESFAR
                     WLRDGFAGELGYMSRTEKLRRDPQELVPWAQSIVSVGMNYYTPNAHPDSSAGSQGSIS
                     RYAWGDDYHDIIQERLERLLEAIRALRGEAIEGRVFVDSGPVLERAFAGVAGLGWIGK
                     NTHLISPKRGSWFFLGELFIDLALAYDRPMRDRCGQCDLCLKACPTQAFVGPYILDAR
                     RCISYLTIELKG"
     gene            complement(763..1206)
                     /locus_tag="VMZ02_08720"
     CDS             complement(763..1206)
                     /locus_tag="VMZ02_08720"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HUR72056.1"
                     /translation="MDANFPRLFTVNEANGLLPLLRPLVEQILENIRRLKGVSETVIR
                     REQLDPDAPDLMERLREDSEILKLVTEIKDWVTTINSHGCVCKGVEQGLIDFPCMLGS
                     EVVFLCWQIGEANVAHYHRIEDGFAGRKTLLDDGSAEPDNKISYH"
     gene            complement(1266..>2379)
                     /gene="lpxC"
                     /locus_tag="VMZ02_08725"
     CDS             complement(1266..>2379)
                     /gene="lpxC"
                     /locus_tag="VMZ02_08725"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HUR72057.1"
                     /translation="MPQVDGIGLLKEIKSQEPDLNVVMVSGHGNIHTAVTATKFGAFD
                     FLEKPVSLEGLLLTVQRALGESSNSAARQGRAKRKTGIKRGDAAASGAPTTLIKQKTL
                     AKSVVLSGQGLHSGVKTGLILHPMPANSGIRFTGISGEVTVPAHLDYVGSTGYATSLR
                     TQGFAIGTVEHLLAVLSCYGIGNLLIKVHGEVPILDGSALEFCQAIEEAGIQQEDEDV
                     AEIVIDRVYRVEAKGGESIVIEPAKEFSVHYELRYPTPVGEQQYFYHHRDGASFRDEI
                     APARTFGFLKDIAKLQTMGLANGGRLSNFILIDDQKIVNTELRFPDEFARHKILDILG
                     DFYLLGRPIRGAIRARMTGHSDNIALLRKVREGMKL"
BASE COUNT          541 a          693 c          644 g          501 t
ORIGIN      
        1 cgcctttgag ctcgatggtt agataagaaa tgcagcggcg cgcgtcgaga atataaggtc
       61 cgacaaaggc ctgggtcgga caagccttga ggcacaagtc gcattggccg cagcgatcac
      121 gcatcggccg gtcgtaagcc aatgccaaat cgataaacag ctcgcccaaa aaaaaccatg
      181 atccccgttt cggagaaatc aaatgggtgt tcttgccgat ccagccgaga ccggcgacac
      241 cggcgaaagc tctttctaac accgggccgg aatcgacgaa gacgcgcccc tcgatcgctt
      301 cgccgcgaag cgcgcgaata gcttcgagca ggcgctccaa tcgctcttgg atgatgtcgt
      361 gatagtcgtc accccaggca tagcgtgaga tcgaaccctg cgagccagcg gaactgtccg
      421 gatgggcatt cggcgtatag taattcatgc cgacggaaac aatcgattgc gcccacggca
      481 ctagctcctg gggatcgcga cgcaattttt ccgtccggct catgtagccc agctcgccag
      541 caaagccgtc gcgcagccaa cgcgcaaagg actccccatg aggcggtaac tgaaccggtg
      601 agatgccgac gagatcgaat cccaactcct gcgcggcatc tttgatcttg gccgagagaa
      661 attctcgcgc cggataagaa ctcgcttcca tgaactcaag cctgcagtat cggagttaac
      721 acggcgcaga aatttgcgcc aagggtggaa ccagaaggcg tgctagtggt aagaaatttt
      781 gttatccggt tccgcgctgc cgtcgtcgag caaggtcttg cgtccggcga aaccatcttc
      841 gatgcggtga taatgcgcca cattcgcttc gccgatctgc cagcagagaa acactacttc
      901 ggaacctagc atgcacggaa agtcgatcaa tccctgttcg acacctttgc atacacagcc
      961 gtggctattg atcgtcgtca cccagtcttt gatttccgtc accagtttta agatctcgct
     1021 gtcctcgcgc aaacgttcca tcagatcggg cgcatcaggg tcgagttgct cgcgccggat
     1081 caccgtttca ctgacaccct tcaagcgccg aatgttttcc aggatctgct ccaccagtgg
     1141 ccgtaacaac ggcaacagtc cattggcttc gttgaccgtg aacaggcggg gaaaattggc
     1201 atccatgtta ggtttctttc aaatttttgg agagcccgcg ccaatatttg cggcgcggtc
     1261 accgttcaca gcttcatgcc ctcgcggacc ttgcgcaaca gcgcaatatt gtccgagtgg
     1321 ccggtcatgc gtgcgcgaat cgcaccgcga atcggtcgac cgagcaaata aaaatcgccg
     1381 agaatatcga gaatcttatg gcgcgcgaat tcgtcgggaa agcgaagctc ggtattgacg
     1441 atcttttggt cgtcgatcaa aataaaattg ctcagccgcc cgccgttggc caatcccata
     1501 gtctgaagct tagcaatatc tttgagaaag ccaaacgtcc gagctggcgc gatttcgtcg
     1561 cgaaacgacg cgccgtcgcg gtggtgatag aagtactgct gttcgcccac cggagtggga
     1621 taacgcagtt cgtaatgcac cgagaactct ttcgccggtt cgatgacgat cgactcgccg
     1681 cccttcgctt caactcgata gacgcgatcg atgacaattt cagcaacgtc ctcgtcttcc
     1741 tgctggatgc ccgcttcctc aatggcttgg cagaattcca gtgcggaacc gtcgaggatc
     1801 ggcacttcgc cgtgaacttt gataagcaga ttgccaatgc cataacagga aagaaccgcg
     1861 agcaaatgct caacggtgcc gatggcaaaa ccttgcgtac gcaacgaagt ggcatacccg
     1921 gtcgagccga cataatccaa gtgcgccggc accgtaactt cgccggaaat cccggtgaag
     1981 cgaatcccgc tgttggctgg catcggatgc aaaatcaatc cggtcttcac gcccgagtgc
     2041 agtccttgcc cggatagaac aacgctcttt gccaaggtct tttgcttgat cagggtcgtc
     2101 ggcgcgcccg atgctgctgc gtcgccgcgc ttgatgccgg tttttctctt ggcacggcct
     2161 tgccgggccg cggaattcga cgattcgcct aaggcgcgct gcacggttaa gagcaggccc
     2221 tccagcgaga ccggcttctc gagaaaatcg aaggcgccaa actttgtcgc ggtcaccgcc
     2281 gtgtgaatgt tgccatggcc cgaaaccatc accacgttga ggtcgggctc ctgagatttg
     2341 atttccttga gtaagccgat gccatcgacc tgcggcatc
//
LOCUS       DATLCH010000297         8159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3421436, whole genome shotgun sequence.
ACCESSION   DATLCH010000297 DATLCH010000000
VERSION     DATLCH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3421436"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..879
                     /locus_tag="VMZ02_08730"
     CDS             19..879
                     /locus_tag="VMZ02_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HUR72058.1"
                     /translation="MLSNKVSEIMTTELVKSPVTASVFSVMETMVVNNVGRIIITDDD
                     VPVGIFTEKDVLKRVVGAQIDVKSVGIRAVMTTPVRAVREETLIVDAFARMYRGKFRH
                     LLVRGRHGKIAGIVSMRRILNIAVELGQGIAESRTLGDIAKAQVVTIDESATAHAAVT
                     LMNRQGVSAVVVTAGGKPAGIFTERDVLTRVATSETDRQNAPLNQVMTAPLVTMPRNA
                     LVGDVLLEMSRRDIRNMPVQDESNQLIGLIAMPEILQYAQAFNIDEQVRRSWQEVAKA
                     LDSDDQYTPG"
     gene            954..2774
                     /locus_tag="VMZ02_08735"
     CDS             954..2774
                     /locus_tag="VMZ02_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HUR72059.1"
                     /translation="METPLINDLNICVATENGSGSASSNNILFKAIFKMGIPCSSKNM
                     FPSNIQGLPTWYQIRANADGYLSRKDVIDVMVMFNDATAAKDIYRVRDGGVLLYDDST
                     PLSPTLKRDGVQYVGFPANKLVAKLVPASPLRAKQRNMVYVGALAYLFGIDMSVIKAV
                     LEDTFGKKPAVIESNLVCIDAGYQYCKEQGFKQNIARLESVPGGNKGKIITEGNTAAA
                     LGAIYGGASVICWYPITPSSSLAESMEYYLPRLRKQIDGKKTYAIVQAEDEIASAGMI
                     VGAGWAGARAMTSTSGPGVSLMNESIGLAYYAEIPCVFFIIQRGGPSTGLPTRTQQAD
                     ISLMYGASHGDTRHIILIPHDVNSCFDLARQSFNYADRFQTPVFVAMDLDLGMNLWSS
                     EPLKLNEEPYDLGKRLSDQDLEEWTKAGKKFRRYFDQDGDGIPYRTVPGNQNRLASYF
                     TRGSGHDADARYSEDEHDYKYILDRLRKKFDTSRKFVPKPIIEKEKGVKTGIICYGSS
                     YEPVREARDRLRASGLKTNHMLIRALPLTSEVMDFLAEHDTVYLVEQNRDAQMAAIIK
                     DENPLLGAKITSILVYNGLPVSAGEVVKQIFDAAQTAKTA"
     gene            2801..3829
                     /locus_tag="VMZ02_08740"
     CDS             2801..3829
                     /locus_tag="VMZ02_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HUR72060.1"
                     /translation="MAEAKVNRLNLTEKDYDGAASTMCKGCGHDAISASVQRAYYESS
                     IDPRKVVKLSGIGCSSKTPAYFLSQSFGFNAVHGRMSAVATGANLANHALLPIGVSGD
                     GDTSAIGLGNFCHMMRRNVNITYIIENNGVYGLTKGQFSATADVGSVMKGGKVNDIPA
                     IDLAELAITLGATYVARSFSGDRKQLTPLVQGALSHKGSAILDVISPCVTFNNHEGST
                     KSLKYVKGHLDPIHDLDFVPPYENIEADYDEGTDHEVSLHDGSRIILHKLGKNYDPTS
                     KSNAIQAIHGAIAESKFLTGLIYYNPNRKEFAEELNLADIPLSELGQDILQPPAEALE
                     RINADMMK"
     gene            complement(3915..4565)
                     /locus_tag="VMZ02_08745"
     CDS             complement(3915..4565)
                     /locus_tag="VMZ02_08745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HUR72061.1"
                     /translation="MDVLRTLKERCDPKNAALIVVDVQNDFVNPDGSAGKRGEDVSAA
                     MAMVPNLLRVIEEARRIGLTIVYIRTTHSEWTDTPSWIYRTSQKSGLSTCREGTWGAE
                     FYQGISPLPSERVVIKHRYSAFINTDLNTVLKARNVQSVLVCGVATNVCVETTARDAY
                     MYDYYVTMIDDCSAAYETKLHLSTLDNVRRHFGLVASSTEIIESWRTLPTQQAPGL"
     gene            complement(4637..5626)
                     /locus_tag="VMZ02_08750"
     CDS             complement(4637..5626)
                     /locus_tag="VMZ02_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HUR72062.1"
                     /translation="MLKVIPVEEAVGLPLAHDITEIIPGKHKGPAFRRGHIIRQEDVA
                     KLLDVGKANIYVMDLEKDELHEEDAARRLAKAAAGANLKLTDPSEGRINMVAAIPGLL
                     KVDADLLYRFNSLGDLMMATLPGDRFVRQGTVVAGTRTIPVLVKDSLVRQAEELCRDR
                     PIVRLLSMPPKHIRLVVTGSEVFTGRIKDGFGPIVNKKVGELGSSVDSVELSPDDPDL
                     IANHIKNAKQAGADVILVSGGMSVDPDDKTPEGIRRSGAKVETHGFPVLPGSMFLLAY
                     LGDTPVMGLSGCVLHDPFTAFDALLPRLIAGEKITRADIMAMGHGGLASKHDH"
     gene            complement(5789..7159)
                     /locus_tag="VMZ02_08755"
     CDS             complement(5789..7159)
                     /locus_tag="VMZ02_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HUR72063.1"
                     /translation="MSIGFEIIVIALLIVGNGLFAMSEMAVITARKSRLQDWVNKGNR
                     RARLALELAQAPNQLLSSVQIGITVIGILTGTFAGRGAAEWMAQYIRALPTIGPYHQQ
                     IGLALVVILITYFSLVIGELVPKRLAMRNPESIATWVAVPLRLFAWLSAPMVQLLSAS
                     TDLVCRLFGQTPSHEPPVTEEEIKNLVQQGTKAGVFEEEAEDMVEAVLRLGDTTARSL
                     MTPRTQIAWLDLESSMEQIRTKISMSGRSRFPVAAGTLDKVTGIVQAKDLLALTLANK
                     PLDLNTLMQQPLFVPRTISALELLESFKKNNKHIALVVDEYGGIEGLLTHHDLLEAIA
                     GDIPVGEKPVDPRAVKRHDGSWLLDGMLSVEEFKEIFHVDMLPGEKRDAYQTLGGFVF
                     TQIGRVPAVAESFEWRGLRFEIVDMDGKRIDKVLVMSKDPTDVMNVHSEKESQSETKA
                     PRGAGN"
     gene            complement(7463..>8159)
                     /gene="chrA"
                     /locus_tag="VMZ02_08760"
     CDS             complement(7463..>8159)
                     /gene="chrA"
                     /locus_tag="VMZ02_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HUR72064.1"
                     /translation="QLAAILSAGIIGWLLFVPTAIVSHDGAQISISKRVGVFAWLLFF
                     ALLLGLPALRPLAQHHELEVFDSFFRVGSLVFGGGHVVLPLLQTEVVVPGWVSNEQFV
                     AGYGAAQAVPGPLFTFAAYLGAVMNGWSGALLTLIAIFLPAFLLIVGALPFWEILRAN
                     KKFQSALSGVNAAVVGLLLAALYQPVWISAIHSRADFAVGLSGFALLMFWKWPPWLVV
                     VLSALAGEVLARA"
BASE COUNT         1961 a         2289 c         2134 g         1775 t
ORIGIN      
        1 tagctcagga ggtgctccat gctgtccaat aaagtcagcg agatcatgac gacggagctg
       61 gtcaagtcgc cggttacggc cagtgttttt agtgtcatgg aaaccatggt ggttaacaac
      121 gttggacgga ttattattac cgacgatgac gttccggtcg gaatctttac cgaaaaagat
      181 gtgctcaaac gcgtggtcgg tgcgcaaata gacgtcaaat ccgtcggcat ccgggctgtg
      241 atgactacgc cggtgcgggc agtacgtgag gaaactctca ttgtcgatgc gtttgccaga
      301 atgtatcggg gcaaattccg ccaccttctg gtacgcggcc ggcatggcaa gattgccggt
      361 atcgtttcca tgcggcgcat actcaatatc gcggtcgagt tagggcaggg gattgccgag
      421 tcgagaacgc ttggcgacat tgccaaggcg caggtcgtaa ccatcgacga atccgcgacc
      481 gctcacgcag ccgtgacttt aatgaatcgt cagggtgtct ccgccgtagt ggttacggcg
      541 ggcgggaagc cggctgggat ctttaccgaa cgcgacgtgc tgacccgcgt tgcaacttct
      601 gaaacggacc gccagaacgc tcctttaaat caggttatga ctgcgccgct ggttaccatg
      661 ccgcgtaacg cactcgtcgg cgatgtcctg ctcgaaatgt cgcgccgtga tattcgcaac
      721 atgccagtac aggacgaatc aaaccaactt atcggtctca tagcgatgcc ggaaattctc
      781 caatacgctc aggcctttaa catcgacgag caggtgcgcc gcagttggca ggaagtcgcc
      841 aaagcgctcg acagcgacga ccagtacact ccgggatagc gacctaaatc gttgtcattc
      901 aaagcgatta tgttaaaatt ggagagttgc cagatatcta aggaggctct actttggaga
      961 caccgttaat taacgaccta aacatctgcg tcgccaccga aaatggctcg ggtagcgcgt
     1021 cgtcgaacaa tattctgttc aaggcaatat ttaagatggg cataccctgc tcatcgaaga
     1081 acatgtttcc ttcgaacatt caggggctgc cgacatggta ccagatccgc gccaacgcgg
     1141 atggctactt gagccgtaag gatgtcatcg acgtaatggt gatgttcaac gatgctactg
     1201 ctgcgaaaga catttatcgc gtgcgcgacg gtggagtgct cttgtacgac gactcgacac
     1261 cgctttcacc gacattaaag cgcgacggag ttcaatacgt cggctttccc gcaaataaac
     1321 tggtcgctaa attggttccc gcttcgccgc tgcgcgccaa acagcgaaac atggtctacg
     1381 tcggagcttt ggcttactta ttcggcatcg atatgtcggt catcaaagcc gttcttgagg
     1441 ataccttcgg gaagaaaccc gcagtcattg aatcgaacct ggtctgtatc gacgctggct
     1501 atcaatattg taaagagcaa ggtttcaagc agaacattgc gcggttggaa tcggttcccg
     1561 gcggtaataa gggcaagatc attaccgaag gcaataccgc agccgctctc ggtgccatct
     1621 acggcggcgc ctcggttatc tgctggtacc caatcacgcc gtcaagttca ctcgccgaga
     1681 gtatggagta ttatttgccg cggttgcgaa agcagatcga cggcaagaag acctatgcca
     1741 ttgttcaagc cgaagacgaa atcgcctcgg cgggtatgat tgttggagcc ggttgggctg
     1801 gggctcgcgc catgacctcg acgtccggtc ctggcgtttc gctgatgaac gagtccatcg
     1861 gtctggctta ttacgccgag attccctgtg tattcttcat cattcaacgc ggcggaccgt
     1921 ccaccggact accgacgcgt acccagcaag ccgacatctc cctgatgtac ggcgcttccc
     1981 atggcgacac ccgccacatc attttaattc cccatgacgt gaactcttgt tttgacctgg
     2041 cgcgccaaag tttcaactac gccgatcgct ttcagactcc ggtattcgtc gccatggatc
     2101 tcgatcttgg tatgaaccta tggtcgtcgg aaccgctcaa gttaaacgag gaaccctacg
     2161 atctgggtaa acgactgagc gatcaagacc tcgaggaatg gaccaaagcc ggcaaaaaat
     2221 tccgccgcta cttcgatcag gacggcgatg gcattcccta tcgaaccgtg ccgggcaacc
     2281 agaaccggtt ggcttcctac ttcacccgcg gttcaggtca tgatgccgac gccaggtatt
     2341 ccgaggatga acatgattac aaatatatcc tcgaccgtct gcgcaaaaag ttcgacacct
     2401 cgcgcaaatt tgttcccaag ccaatcatcg aaaaagaaaa aggcgttaaa accggtatca
     2461 tttgttacgg ctccagttac gagcccgtac gcgaggcgcg cgatcgtttg cgcgcctccg
     2521 gtctcaaaac caaccacatg ctcattcgcg cgctgccgct taccagtgaa gtgatggatt
     2581 ttttggcgga acatgacacg gtgtatctgg tcgaacaaaa ccgcgatgcc caaatggcgg
     2641 cgatcataaa agatgagaac ccgctgttgg gtgcgaaaat taccagcatt ctggtgtaca
     2701 acggcctgcc ggtgagcgcc ggcgaagtcg tcaaacagat ttttgacgcg gcacaaaccg
     2761 cgaaaaccgc ctaacgataa tcatataaga aggattttat atggcagaag caaaagtaaa
     2821 tcgtctcaat cttacggaga aggactatga cggcgcagcc tcgaccatgt gcaagggttg
     2881 cggccacgac gccatctccg cgtcggtcca gcgcgcttat tatgaaagca gcatcgaccc
     2941 gcgcaaagta gtcaagctca gcggcattgg ttgctcgtcg aaaaccccgg cttattttct
     3001 aagccaatcc ttcggattta atgccgttca tggtcgcatg tcggcggtcg ccacgggcgc
     3061 caacttggcg aatcatgcgc tgctcccgat cggcgtctcc ggcgacggcg atacgtcggc
     3121 cattggcctt ggcaacttct gccacatgat gcggcgcaac gtgaacatta cctacattat
     3181 tgaaaacaac ggcgtctacg gcttgaccaa gggacagttc tccgccaccg cggatgttgg
     3241 ttccgtcatg aagggcggca aagtcaacga cattcccgcc atcgacttgg ctgagttggc
     3301 gattacgttg ggcgctactt atgtcgcccg gagcttttcc ggcgatcgaa agcagctgac
     3361 gccgctggtt cagggcgctc tgtcgcacaa aggcagtgcg atcctcgacg tcatcagccc
     3421 ctgcgtgact ttcaataacc atgaaggatc aaccaagagt ctgaaatacg tcaaaggcca
     3481 tctcgatccg atccacgatt tagatttcgt gccgccttat gaaaatatcg aggccgatta
     3541 cgatgagggc accgatcacg aagtttcgct gcacgacgga tcgcgcatca tcttgcacaa
     3601 gcttggcaaa aattacgatc ccacaagcaa gtctaatgcg atccaagcca tccatggcgc
     3661 gattgccgag agtaagtttc tgactggctt aatttattac aatcccaacc gcaaggagtt
     3721 cgccgaagag ctcaatcttg ccgacatccc attgtcggaa ttgggtcaag atatcctgca
     3781 accaccggcc gaagctttgg aacgcatcaa tgccgacatg atgaagtaag ccgacgacaa
     3841 agatatgaat agaaaaaagc ccggcagacg ttctgccggg cttttttatt gcttcgaaca
     3901 catctgaagc ggagttaaag ccctggcgcc tgctgggtcg gaagagtccg ccaggattcg
     3961 atgatctccg tggaagaggc gactaagccg aagtgacggc gcacgttgtc gagggtgctc
     4021 aaatgcaact ttgtttcgta cgcggccgag cagtcgtcga tcatcgtcac gtaataatca
     4081 tacatgtaag cgtcgcgcgc cgtggtttcg acgcagacat tggtggccac gccacaaacg
     4141 agcacgcttt ggacgttgcg cgctttgagc acggtattga ggtcggtgtt gatgaatgcg
     4201 ctatagcgat gcttgatcac gactcgctcg gacggcaagg gtgaaatgcc ttggtaaaac
     4261 tccgcgcccc aagttccttc ccggcaggtg ctgagcccgc ttttttgcga ggtgcgatag
     4321 atccatgacg gcgtatcggt ccactcgctg tgagtggtac ggatataaac gatcgtcaga
     4381 ccaatccgcc gcgcctcttc gatcacgcgc aaaagatttg gcaccatcgc catggctgcg
     4441 ctgacgtctt cgccgcgctt gccggcgctg ccatcgggat tgacgaagtc attttgcacg
     4501 tcgacaacga ttaatgccgc gtttttcgga tcgcagcgct ctttcaacgt tcgtaatacg
     4561 tccacataca cctcgtgttt tggaacgaga atgggccagt tagatgatcc attctcgttg
     4621 tcgattttcg ctccggtcaa tgatcgtgtt tactcgccaa gccaccatga cccatcgcca
     4681 tgatatcggc acgagttatt ttttcaccag caatcaagcg cggcaacaga gcgtcgaagg
     4741 cggtgaacgg atcgtgcagc acgcagccgg acaaacccat caccggcgtg tcgcccaggt
     4801 aagccaacag gaacatcgag ccaggcagca cagggaatcc atgggtttcc actttcgcgc
     4861 cgctacgccg aatgccctcg ggggttttgt cgtcagggtc gacggacata ccgccactca
     4921 ccagaatcac atcggcaccc gcttgcttgg catttttaat atggttggca atcaaatcgg
     4981 gatcgtccgg cgacaactcc acggaatcga cactggaacc cagctcgccg acttttttat
     5041 taacaatggg gccgaacccg tctttgattc ttcctgtgaa aacctcgctg ccggtcacga
     5101 ccaggcgaat atgcttaggg ggcatcgaca gcaaccggac gatcggccga tcccggcaca
     5161 actcttcggc ttggcgcacc agcgagtctt tgacgagtac gggaatggtc cgcgttcctg
     5221 ccaccaccgt accctgccgc acgaagcggt cacccggcag ggtcgccatc atgagatcgc
     5281 caagggagtt gaacctgtag agcaggtcgg catcgacttt caataggccg ggtatagccg
     5341 ccaccatatt gatccgtccc tcacttggat cggttagttt taaattcgca ccagctgccg
     5401 ctttcgccaa gcgccgcgcc gcgtcttctt catgcagctc atctttttcc aaatccatga
     5461 cgtaaatatt ggctttgccg acgtcgagca atttcgccac gtcttcctgg cgaataatat
     5521 ggccgcgccg gaaagccggc cccttatgtt tgccgggaat aatttccgta atatcatgag
     5581 ctagcggcaa cccaaccgct tcttccactg gaatgacttt gagcataaca acgactcctc
     5641 gcaactacct ctattaaatc cgcggtgatt tttccacctg gtagtagctt tatccagacg
     5701 gcgtgtgtta tgcggcgaaa actatgaaag tcgcagaact cacagtaaaa acagcaaatt
     5761 cagtaatcac tgtagcgccg agtagcattc aattgcccgc gccgcgcggt gcctttgttt
     5821 cagattgtga ctctttttcg gaatgcacat tcatgacgtc cgtagggtcc tttgacatga
     5881 ccaatacttt gtcgatgcgc ttgccgtcca tgtcaacaat ttcgaatcgc aagccacgcc
     5941 attcgaaaga ttccgccacg gcgggtacac ggccaatttg agtaaatacg aaacctccga
     6001 gggtctggta ggcatcgcgt ttttcgcccg gcaacatatc tacatgaaat atttctttga
     6061 actcctcgac gctcaacatg ccatcgagca accaggagcc gtcatggcgc ttgaccgctc
     6121 tcgggtcaac cggtttctcg ccgacaggaa tgtcgccggc gatcgcttca agtagatcgt
     6181 gatgggtcaa caatccctcg attccgccat actcatcgac cacgagagca atatgcttgt
     6241 tattcttctt gaaggattcc aatagttcca acgcggaaat cgtccgcggc acaaacagcg
     6301 gttgctgcat caaggtattg agatccagcg gtttattggc gagcgtaagc gccaacaaat
     6361 ctttcgcctg gacgatgccg gtaactttgt cgagggtgcc agccgcgacc ggaaaccgcg
     6421 atcggccgct catgctgatc ttggtgcgga tctgttccat actgctttcc aagtcgagcc
     6481 aggcgatctg agtacgcgga gtcatcaatg accgagccgt ggtatcgcca agacggagca
     6541 cggcttcgac catgtcctcc gcttcttctt caaaaacacc ggccttggtg ccttgctgca
     6601 ccaggttttt gatttcttcc tcggtgacgg gcggctcgtg agatggcgtc tgaccgaaaa
     6661 ggcggcagac caaatcggtt gacgcactca gcaactgcac catgggcgcc gacagccagg
     6721 caaacaaacg aagcggcacc gccacccagg tggcgatcga ttccgggttg cgcatggcta
     6781 gtctcttggg aaccaattcg ccgatgacca gagaaaaata ggtgatgagg attacgacca
     6841 aagccaaacc tatttgctga tgatacggtc caatggtcgg cagcgctcgg atatactggg
     6901 ccatccactc tgcggcgcca cgaccggcga atgtgccggt gagaatgcct atcactgtga
     6961 tgccgatttg caccgaggag agcaactgat tcggcgcttg cgcaagctcg agtgccaatc
     7021 gcgcgcggcg gttgccttta ttgacccaat cttgcagccg cgacttgcgc gcggtgatca
     7081 cggccatctc cgacatggcg aatagaccat tgccaacaat caagagggca atcactatga
     7141 tctcgaatcc gatggacatt cgttcactcc ttgcgcgata acgcgaaacc ggcgtcggga
     7201 aaattttaac gtgcagccag cgtcaaacaa gccaacagcg gatgcttgcg aatgggccat
     7261 ccctcttcga gatttcctgt tcgcttatga catagcgtcg cacgagtcat aaaggtcttg
     7321 gaagcacaca gaaaaaatcg cactgtaaag gattactcgt gcgacgatgt cgacggcgcg
     7381 aaggcctagc ttcggcgcag cttactatca attttgcaca ctactctcgg ttattctgcg
     7441 cgcatcggcg acgtgcaaag gttcaggcgc gcgcgagcac ctcgccagca agcgcgctta
     7501 gaacaacgac cagccacggc ggccacttcc aaaacatcag taatgcgaat ccgctcaagc
     7561 caaccgcgaa atcggcgcgc gagtggatcg cactgatcca caccggttga tacagcgccg
     7621 cgagaagcag accgacaacc gcagcgttga caccgctcag agctgattga aactttttat
     7681 tggctctaag gatttcccaa aacggtagcg cgccgacaat tagcaaaaac gccggcagaa
     7741 agatcgcgat gagcgtcaac aacgcaccgc tccagccatt catcaccgca ccgagatatg
     7801 ccgcaaaggt aaacaaaggg ccggggaccg cctgcgcagc tccataacca gcgacgaatt
     7861 gttcgttgct aacccaaccc gggacgacca cctcggtttg cagcaacggt aaaactacat
     7921 gtccgccgcc gaaaaccagc gaaccgacac gaaagaaact atcgaagact tcgagctcat
     7981 gatgctgcgc caacgggcgc agcgccggca gcccgagcaa aagcgcgaaa aaaagcagcc
     8041 atgcaaacac gccaacgcgt ttgctgatag aaatttgcgc tccgtcgtgc gacacaatcg
     8101 ccgttggcac aaataataac caaccgatga ttcccgccga aaggattgcc gcgagttgt
//
LOCUS       DATLCH010000298        10477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10855550, whole genome shotgun sequence.
ACCESSION   DATLCH010000298 DATLCH010000000
VERSION     DATLCH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10855550"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VMZ02_08765"
     CDS             complement(<1..410)
                     /locus_tag="VMZ02_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72065.1"
                     /translation="MKESIELKLTKRIVELGEDVIVSIPDAVKDDARAPKLTDHTARA
                     RAMTVAMLGLLAMPLCGLMAGDENLWERLQALIESQRATQTQSTVLAVDTAGLLVRSK
                     SSSPATKELFEQAAMEHLARLQHTFNSWSERNQDL"
     gene            complement(716..1234)
                     /locus_tag="VMZ02_08770"
     CDS             complement(716..1234)
                     /locus_tag="VMZ02_08770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR72066.1"
                     /translation="MLEPKQKPKIRSKKRPKITVKRVSSAAELSRAYAIRIRVFVKEQ
                     NVPREVELDADDGRAIHFLAMDCVRTIGTARVVLRRHAAKIGRMAVLKSWRRKGVGAK
                     LLTRAIAQARKMKARKIYLHAQVAVSGFYQRMNFHACGPVFDEAGIAHRKMVWRPPPT
                     NVRRRNAKKVKV"
     gene            1464..1784
                     /locus_tag="VMZ02_08775"
     CDS             1464..1784
                     /locus_tag="VMZ02_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HUR72067.1"
                     /translation="MKQTCFVCQNVDCQSRGSEALMKELTDMVAAKAIDAEVKAYICF
                     GGCDFGPNVVVHPQKNWYAGVKKEDLPEIVNSLAGGPAVARLDTIDPSLKDIVYSLLD
                     AGVV"
     gene            complement(1909..2742)
                     /locus_tag="VMZ02_08780"
     CDS             complement(1909..2742)
                     /locus_tag="VMZ02_08780"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR72068.1"
                     /translation="MTNNNAYEIEEQDVEYQRLAGKPWLARIYRPKGPGPFPSIIDVH
                     GGAWHNGDRLNNAGVDRALAAKGIVVAALDFRQPPEASYPASLCDVNLAVRWLKAHAA
                     EFNGTATVGAFGNSSGGHQVVLSALRPRHPAYSMLPLGDDGSVDASVNYVIAAWPVIC
                     PLYRFRYAKRIDNQQHIQAHIDYWRSEEAMSEGSPQTIIESAEVFELPPVLFLLKQDD
                     KNHPLEMQERFIASYRKRGGAIEVHTFAGLPEHRMVPSPEQPETLRFIDIVSGFITAK
                     A"
     gene            2977..4539
                     /locus_tag="VMZ02_08785"
     CDS             2977..4539
                     /locus_tag="VMZ02_08785"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR72069.1"
                     /translation="MTSKELILTPAEAFDDTSFIPALYNKLAIPQDGSLVVVPRKRSV
                     DARGRDIKVRVQYDIVERTGLAPPRTKDYPPAHAKEPVLIIGSGPAGLFAALRLVELG
                     YKPIVLERGKDVQARRYDLAAINKEHTVNPDSNYCFGEGGAGTYSDGKLYTRSKKRGD
                     VQRIMEILVAHGAKDEILFDAHPHIGTNKLPKLVTELRESIKQAGGEIHFQTRVTDFV
                     VAGSELTGVRTQSGDVVEGRAVILATGHSARDIFHLLNQRKIRIEAKPFALGVRVEHR
                     QCLIDQIQYHCSDRGAYLPAAAYGLVHQAKINGKDKGVFSFCMCPGGFIVPAATAPGE
                     LVVNGMSPSQRNARFANSGIVVAVDENDYRHLRRFGALAALQFQAEVEQKAWEVGGKT
                     QAAPAQRLSDFVAGKPSASLPETSYHPGLTCADLRAFLPSFIADALQQGFKRFGEKMP
                     GYLTSDAQLIGVESRTSSPVRIPRDAVTLQHPQIGNLFPCGEGAGYAGGIVSAAMDGE
                     RCAEAVMRLCKS"
     gene            5071..5265
                     /locus_tag="VMZ02_08790"
     CDS             5071..5265
                     /locus_tag="VMZ02_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72070.1"
                     /translation="MNRSSKKQLNLGDLIYVLTEAVQRHIRDEKLVYEIVANLLAGMV
                     AARGKINYSGIFTEPARLAS"
     gene            5318..5938
                     /locus_tag="VMZ02_08795"
     CDS             5318..5938
                     /locus_tag="VMZ02_08795"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72071.1"
                     /translation="MGLSKKTVLAVVAAGVLFSTVASRVQAGVPTEQVRQTADKVLQV
                     LQDPQSKSNSNAAQRREQLTQILASRFDFAEMAKRSLGANWQKGSASEQQQFVRLFTN
                     LLESSYIGQIEAYSGEKIIYGRETVEQNQADVETKIVTKKSEEVSVNYKLKGDGGNWK
                     IYDVVIENVSLVNNFRNQFNRMLAKGSFADLISQLESKSTLKPDRS"
     gene            6007..6351
                     /locus_tag="VMZ02_08800"
     CDS             6007..6351
                     /locus_tag="VMZ02_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HUR72072.1"
                     /translation="MKRSHNKPALLLTATLLFGGCAGGSLTTREKGAGIGALGGAAVG
                     GIVGSAVGHPGAGAAIGGGLGLGAGALVGDQLQGQQNQNNQQDNQIRNNQAEIDRQRY
                     ELERLRRERGEY"
     gene            complement(6545..6796)
                     /locus_tag="VMZ02_08805"
     CDS             complement(6545..6796)
                     /locus_tag="VMZ02_08805"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HUR72073.1"
                     /translation="MRSETTVAIPAAESPQDQVLSKLVRDRLVADKKVDLSGIKVVSN
                     SGTVYLSGFVHSLDARQQAIKLAWEVRGVQSVVNSLEVK"
     gene            7220..7603
                     /locus_tag="VMZ02_08810"
     CDS             7220..7603
                     /locus_tag="VMZ02_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR72074.1"
                     /translation="MSRVMEGPSIKRPPFAAPFLKAGKLVIAQTAYILVFLGPRLGLA
                     PKQEAGRLWAHQLQLTIADFVREVHDSHHPIGGGLYYQDQKREAKRRAKDFTAERLPK
                     FLGYFEQALKRQWRPYTRRQRCFLR"
     gene            7572..7904
                     /locus_tag="VMZ02_08815"
     CDS             7572..7904
                     /locus_tag="VMZ02_08815"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HUR72075.1"
                     /translation="MLVGNAVSYVDLSLFQMIEGLRYAFPRAMTRQERKHRCVIALHD
                     RVAARYRLAAYLESERRLPFNQDGIFRHYPELGRSVVISMPGAGYRAKAASHYVIPVL
                     ITAQKWQL"
     gene            complement(8213..9211)
                     /locus_tag="VMZ02_08820"
     CDS             complement(8213..9211)
                     /locus_tag="VMZ02_08820"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72076.1"
                     /translation="MTKRALIILLLVSLRPDFRAAYADATKLIVAYASPAATFCPGWI
                     ARREGIFAKYNLDVEMVLMQGASAYMPALANGNIQVLYGGGTAVSRAIATKDFDFMVI
                     ATETRYVPVRLMVNSSIRTPADLKGKKLGAGLAGLDEYATLLYLDKLGLVAGRDVQMV
                     YLAGGIPSRVAAMKQGLIDGVAVNPPNEYELEKAGYRELVNFLDLKMPYAGVPHTVSR
                     NFRDRNRRVLEDYMTAHVAGMQLFRTHKQLAFKAITELTRQKDPVILERTYDTYSKQY
                     EAIDGLPIPWQAGIESMITGFHERFNPQGIKNRDASAALDASFVQKAAERLKFGKK"
     gene            complement(9474..>10477)
                     /locus_tag="VMZ02_08825"
     CDS             complement(9474..>10477)
                     /locus_tag="VMZ02_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72077.1"
                     /translation="LWIEELGMHTVKNPKSKQTRKSISALILLLQLFLGLAHAQDREK
                     IRVALSTVSFTFLVNLIAKDAGIFRKHGLDVESILVSGPAQVAALAAGEIDYNSSFIP
                     GLLLAAKGLPATIVMATTKTPLFYIVSQPDITKIDVLAGKKVAVSRIGSQSHNLTRSM
                     LKQGGINPDAVTYIQTGSAANAFSALNAKAVDAAALSIPFNILMKQKAFNQLASTQSI
                     GVYPNTGLQVLKTKVEKNRGQVKAMIRAFLDSLRYIANEKGKVSDFIQRTWKLSPDVV
                     EQTYKEFLPSLPMDGKIGVEAVQEYLDSAYRSREIPQRVNVNALVDYSILEEVLKER"
BASE COUNT         2473 a         2910 c         2736 g         2358 t
ORIGIN      
        1 agatcttgat tgcgctcgga ccagctgttg aaggtatgct gcaatcgggc caggtgttcc
       61 atcgccgctt gctcgaataa ctctttcgtc gccggcgacg aactctttga acggacaagg
      121 agtccagcgg tatcgactgc caatacagtg ctctgtgtct gtgtcgcccg ttggctttcg
      181 attaacgctt gcagtctctc ccagagattt tcatctccgg ccatcaggcc gcaaagtggc
      241 atcgccaaca gtcccagcat ggccaccgtc atggcacgcg ctcttgccgt gtggtcggtt
      301 agcttgggcg ctctggcatc atccttcacg gcatcgggta ttgaaacgat gacgtcttcg
      361 ccgagttcga cgattcgttt agttagtttg agttcaatgc tttctttcac gtcttcctcc
      421 ctttcgactc gatagcggaa ccatggcgcg gaatggtgca ggggcgcgcg ctgagtagct
      481 ggaaatttcg cgttttagtt tgacttaagt aggtagcaac gacgaggcca gttgagcttc
      541 ggtacggcag gtaagaaatg tgcgatactg gaagcttgag gaattgtcca cgaaccgaaa
      601 tggaccgtta ttgcaaagat tccgaactag ccgctgctcg gacaattctg tgtcaattcg
      661 cgatttccgt cacagagcta ctcactgcag cgctcgtttt gcgaaacctg caaaactata
      721 ccttcacctt cttggcattc cgtcggcgga catttgtcgg cggggggcgc caaaccattt
      781 ttctatgagc aatgccggcc tcgtcaaaca ccggtccaca agcgtgaaag ttcatgcgct
      841 ggtaaaatcc gctgacggca acctgcgcgt gcaagtagat cttgcgcgcc ttcatctttc
      901 gtgcttgcgc gatcgcacgc gtcaataact ttgcaccgac tcctttacgc cgccagctct
      961 tcagcaccgc catgcggccg atcttggcag cgtgccgacg caataccact cgtgccgtac
     1021 ctatcgttcg gacgcaatcc atggcaagaa aatggatggc cctaccatcg tctgcatcca
     1081 gttctacctc gcgtggcacg ttctgctcct tgacgaaaac ccggatacga atggcgtacg
     1141 ctcgcgaaag ttccgccgcg ctagaaactc gcttcacggt tattttggga cgtttcttag
     1201 atcggatctt cggtttttgc tttggctcga gcatgtttgc tgcttgtccg gcttcgctat
     1261 ctatcacgcc ggcagtaggt ccgaaagatc gctctacggc ttcgccagta taagttttat
     1321 gtttggtaac taaggataaa tattaactga gggccctcac acggatgcta tgaaatcgaa
     1381 acacgacttt gctcctcgtg atgatgttga cactggccaa agttctcaga tatttacact
     1441 agtgaaaagg ggatatcatc accatgaaac aaacttgttt tgtctgccaa aatgtcgact
     1501 gccaaagccg cggttcggaa gccctcatga aagaactcac cgatatggtc gccgccaaag
     1561 cgatcgatgc cgaagttaag gcgtatattt gcttcggcgg atgcgacttc ggtcccaacg
     1621 tcgtcgttca cccgcagaag aactggtatg cgggcgtcaa gaaagaagac ctacccgaga
     1681 ttgtcaactc actcgcgggc ggcccagcag tcgctcggct cgacaccatc gatccgtcgc
     1741 tgaaagatat cgtgtactcg ctgttggatg ccggcgtcgt ataattgtga ccggtgccaa
     1801 cgatgaaatc gacttccggt tccagggatt gttacaagtg tcgaaaccga gtcaatttcc
     1861 cagcgagagc cggcaagaaa ctcttctccc gttgccaata gttcgtggct aggcttttgc
     1921 cgtaataaat ccactgacga tatcgatgaa gcgcagcgtt tccggctgtt ccggcgaggg
     1981 caccatgcgg tgctccggca ggccggcaaa cgtatgcacc tcgatcgccc caccgcgttt
     2041 tcgatacgat gcgataaagc gttcctgcat ttccagtgga tggttcttat cgtcttgttt
     2101 aagcaagaac aaaaccggcg gcagttcgaa cacctcggca ctttcgataa tcgtctgagg
     2161 actaccttcg gacatcgctt cttcgctacg ccagtagtcg atgtgggctt ggatgtgttg
     2221 ttggttgtcg atccgcttgg cgtaacggaa gcgatagagc gggcagatca ctggccaagc
     2281 agcgatgaca taattgacgc tggcgtcaac cgaaccgtca tcacccagcg gcaacatcga
     2341 ataggcagga tggcgcggcc gcagcgcgct aagcaccact tgatggccgc cgcttgagtt
     2401 accgaacgcg ccgaccgtcg ccgtgccgtt gaactcagcc gcatgggcct tgagccagcg
     2461 cacagccaag ttcacgtcgc aaagcgatgc cggataggac gcctccggcg gctggcgaaa
     2521 atctaacgcc gccacaacga tgcccttcgc cgccagcgcc cgatcgactc cggcattgtt
     2581 caaccgatca ccgttgtgcc acgcaccgcc gtgcacgtct atgatgctcg gaaacggccc
     2641 aggtcccttg ggccgataga tgcgtgccag ccaaggtttg cccgccaacc gttgatactc
     2701 gacatcctgc tcttcaatct cgtatgcgtt attgttagtc atggcgcgaa tctaacgccg
     2761 ggttaagtga aactgcaagc ccaaaacagt gattggaccg gcgcggaagc ctatctgctg
     2821 caattcgtca tgcaccgccg cggcattcac tgactctgtc ggccagcaat ggcggaaaat
     2881 tttcgctgta atatcagcca ctgccgtccg ccatgttata actgatggag tggaatcgcg
     2941 gcgccgactt ttgccggatc aaacctacgt taaaaaatga ctagcaaaga attaattctg
     3001 acaccggcag aagccttcga cgatacttca ttcattccag cactctataa caaactcgcc
     3061 ataccgcagg atggcagttt agtcgtggtg ccgcggaagc gttcggtgga tgcacgcggc
     3121 cgcgacatca aagtccgcgt gcaatacgac attgtcgagc gcaccggctt ggcgccaccc
     3181 cgcaccaagg actacccccc ggcccatgcc aaagaaccgg ttcttattat cggttcggga
     3241 ccagcggggt tgttcgccgc actccggctg gttgaattgg gctacaaacc catcgtgctc
     3301 gagcgcggta aggatgtgca agcgcgccgc tatgatctcg ccgccatcaa caaagaacac
     3361 accgtcaatc cggactcaaa ctattgcttc ggtgaaggcg gggcggggac ttattcggac
     3421 ggaaaacttt atacgcgctc caagaagcgc ggcgatgtac aacgcatcat ggagatactc
     3481 gtcgcccacg gcgccaagga cgagatcctc ttcgacgccc atccgcatat cggcaccaat
     3541 aagttgccca agctcgtcac cgaattgcgc gagagcatca aacaagccgg cggcgaaatt
     3601 catttccaaa ctcgcgtgac ggacttcgtg gtcgccggca gcgagctaac cggcgtgcgc
     3661 acacagtcgg gcgacgtcgt cgaaggccgg gcggttattt tggctaccgg ccactccgcg
     3721 cgcgatattt ttcatttact caaccaaaga aagatccgaa tcgaagccaa gcctttcgcg
     3781 cttggcgtac gagtggagca ccgccagtgt ctgatcgatc aaattcaata ccattgctct
     3841 gatcgcggcg cgtatttgcc tgcggcggcc tatggtttgg tgcatcaggc caagatcaat
     3901 ggcaaggaca agggagtgtt ttcgttttgc atgtgtcccg gcggctttat cgtcccggcg
     3961 gcgaccgcgc caggcgagtt ggtcgtcaat ggaatgagcc cttcgcaaag aaatgctcgc
     4021 ttcgccaact ccggcattgt cgtcgcagtc gacgaaaatg attatcgaca tctgcgccgc
     4081 tttggcgctc tcgccgccct gcagtttcag gcagaagtcg aacaaaaagc ttgggaagta
     4141 ggtggcaaaa cccaagccgc accggcccag cgcctgagcg atttcgtagc gggaaaaccc
     4201 tcggcgagtc tacccgagac ctcgtaccac cctggcctaa cttgcgccga tctgcgcgcg
     4261 ttcttgccga gttttatcgc cgacgctctt caacagggat tcaaacgctt cggagaaaaa
     4321 atgcctggat atcttaccag cgacgcccaa ctcatcggcg tagagagtcg aacctcctcg
     4381 ccagtgcgca tcccgcgcga cgcggtcact ctgcaacacc cgcaaattgg aaatttattt
     4441 ccctgcggcg aaggcgccgg atatgcaggc gggatcgtct cggcagccat ggacggcgaa
     4501 cgctgtgccg aagcggtcat gcgtctgtgc aaaagttgat aaaaagttac tagacaaaag
     4561 ctacaagcgg cgtcgaaccg tcccattaat gaaacaaatg taacaatgca agtgtcgcgt
     4621 tgagttgtgc atctggatca ctccgaccta ccgcccgact ccggccagca tcaattctcc
     4681 cttgatctgt ttccctgtgc tgagggtgac gaatagacga ggaacgtggt tctcgcgccc
     4741 tacagatgcg gcgaacaagc gctttcaccc cgtttaacgt agctgcgggt taaatgggaa
     4801 gcaattaccg agtaaaaatt acctgtcaaa atttatcggt aaataaatcc tccaggcttc
     4861 aactcgccaa tttaaaggca aaaccaaaac aacgtcaccg attgccgcag gtaaatccat
     4921 acaagctagc agttgcccaa ttctcgggcc gataatcaaa agttatccag atttggcgta
     4981 gttacggcga atctcgccca gtggtacgag ccttgcgggt agttcctgac gcaaaggaac
     5041 aatcaaacgg aaaacgttgt gggggtaaca atgaatcgtt cgtcaaaaaa acaactaaat
     5101 ctcggcgacc tgatttacgt attaaccgaa gcagtgcagc gccacattcg cgatgaaaaa
     5161 ctcgtttacg aaatagtcgc caatttactg gccggcatgg tcgccgcgcg cggtaaaatt
     5221 aactattctg gaatctttac cgaaccggcg cgactggcat cgtaacgaaa atcataatga
     5281 acgtacgtgg aaacatagca atcttaatgg gggaattatg ggcttatcca aaaaaacagt
     5341 tttagcagtt gtcgcagcgg gggtattatt ttctacggtc gcaagtcgcg tgcaagccgg
     5401 cgtaccaacg gaacaagtgc gccaaacggc agacaaggta ttgcaagtgc tgcaagaccc
     5461 gcagtcgaaa tcaaatagca atgcggccca acgccgtgaa caactgacgc aaattctcgc
     5521 cagtagattc gactttgccg agatggccaa gcgttccctc ggcgcaaatt ggcagaaagg
     5581 cagcgccagc gaacaacaac aattcgtccg tctttttacc aatctcttgg agagttccta
     5641 tatcggccaa atcgaagcct acagcggcga aaaaatcatc tatggacgcg agacggtcga
     5701 gcaaaatcag gccgatgtcg aaactaaaat cgtcactaag aaaagcgaag aagtatcggt
     5761 aaactacaaa ctcaaaggtg atggcggtaa ttggaaaatc tacgacgtag tgatcgaaaa
     5821 cgtaagtctc gtgaataact ttcgcaacca gtttaatcgc atgttggcca aaggttcgtt
     5881 cgctgacctg attagccagt tggaatcgaa gtcaactctt aaacccgacc gtagttaaat
     5941 tgccgataga gaattaaccg acccaatcaa aaccgaagga ccttcactaa tgcgaaagga
     6001 gactttatga aacgctctca caataaacct gctcttctac tgacggcaac attactgttt
     6061 ggcggctgcg ccggcggctc gctgacaacg agggaaaagg gcgccggcat cggcgcgctc
     6121 ggcggcgctg ctgtcggcgg tatcgtcggg tccgcggtcg gacatccggg cgcgggggca
     6181 gccattggcg gcggcctcgg tttgggtgcc ggcgcgctag tgggcgatca gctccagggg
     6241 cagcaaaatc aaaacaacca gcaagataat cagatccgca acaatcaagc ggaaattgat
     6301 cgccagcgct atgaactcga aaggctgcgg cgtgagcgcg gcgaatacta gttgtcttta
     6361 atcatccgct acttttgtgc gtcgaacccg cgcgtcgggt tcgacgcaca actatattaa
     6421 gtacatcttc atcccaagtg atccggggtc gcttatcgca gcaatttttt tgggttgggc
     6481 aaaagtgcgg ggtaaagact caccgcgagc gcagcagagt ctgcacattc taatctatag
     6541 gtaattattt cacttccaga ctattgacga cgctctgtac gccgcgcact tcccaggcaa
     6601 gttttatcgc ttgctggcgg gcatcgagcg aatgcacaaa gccgcttagg tagacggtcc
     6661 cgctgttgga tactaccttg atccctgata gatccacttt cttgtccgcg acaagccggt
     6721 cccgcactaa cttggagagc acttgatctt gcgggctttc cgccgctgga attgccacgg
     6781 tggtttcgct gcgcatggtt tggcagccta aagcaagcgg cagcactgct aacacgagcg
     6841 caagaaagga ttgtcgagtc gatcgcatag cgcattcgat atcacagacg gcgatttctt
     6901 actagaaaga acttctggcg aacgttgaag ggctgatcgc cgcgagcatg tacctcggac
     6961 aaccagccgc cctgatatga cccacccctc aattcttaag cgctagttgg ctctctttgc
     7021 tgaccgcagg aacgcgcgtt ggagcatctt gcgtagagaa tataatttaa cctcaggtga
     7081 agagtctctg agagcttgcg gagggcaatt atgagctaca aactgtatta ttggcctggc
     7141 ataaaggacg cggctcgctt tggaagacgc cggcgcggac tacctagaag ttgcccgcca
     7201 accccgaggg atctcggcga tgtcgcgcgt tatggagggc ccatcgataa agcgaccacc
     7261 gttcgcggcg ccgtttttga aagccggtaa gctggtcatc gctcagaccg cctacattct
     7321 cgtctttctc ggcccgcgcc tgggcctcgc gccgaaacaa gaggccggca gactgtgggc
     7381 ccatcaactg caactgacga ttgcggattt tgtcagggag gttcacgaca gccatcatcc
     7441 gatcggcggc ggcttgtatt accaagatca aaaacgcgaa gccaagcgcc gcgccaagga
     7501 tttcacggcc gagcgtctgc cgaagtttct cggctacttc gagcaagcct tgaaacgcca
     7561 atggcggccg tatactcgtc ggcaacgctg tttcctacgt tgatttatct ctcttccaga
     7621 tgatcgaagg cctgcgctac gcatttcccc gcgccatgac caggcaggaa cgcaagcatc
     7681 ggtgcgtcat cgcgctgcac gaccgagtcg cagcgcgcta ccgcctcgca gcgtacctgg
     7741 agtccgagcg ccgcctgccc ttcaatcagg acggcatctt tcgtcactat ccagagttgg
     7801 gacgaagcgt cgtgatatct atgcccggcg ctggctaccg agcgaaagcc gcatcacact
     7861 atgttattcc ggtattgata actgcgcaaa aatggcagct ttagggagca cggggcagcg
     7921 tactcgggag atcattttta gtaacttgcc gcgcgccatc agctgaggaa agaagctttt
     7981 ctcgaaccgg aagtacgact cgatactctc gggcgttgaa ggtttccagg attgtccgcc
     8041 tcgttaacgg gggcgacctt ttctactgat cttcagcggg gtacgaatca taacaacctc
     8101 atccataaat cggtaaagat ccaaaccctt ttgagcaaca agtgatcgat gggattcttg
     8161 ccggcaggtt gtgctacgcc atgactagct gcccgattct tttcacttcc gattacttct
     8221 tgccgaactt caaccgctcc gcggctttct gcacgaatga tgcgtcgagc gcagccgaag
     8281 catcgcggtt tttgatcccc tgcggattaa atcgttcgtg gaagccggtg atcatgcttt
     8341 cgataccggc ttgccaggga atcggcagac cgtcaatggc ttcgtattgt ttggaatacg
     8401 tatcgtaggt acgctcgaga atcaccggat ctttttgccg ggtcaattca gtgattgctt
     8461 tgaaagccaa ttgcttatga gtccgaaaca actgcatgcc agcgacatgc gcagtcatgt
     8521 aatcttccag cacgcgccga ttgcggtcgc gaaaattccg gctcacggtg tgcggcacgc
     8581 cggcataggg catttttaaa tcgaggaagt tgaccagctc gcgatacccg gctttttcga
     8641 gctcgtactc attgggcgga ttcacagcga ccccgtcaat cagcccctgc ttcatcgcgg
     8701 cgacccggct aggaatgccg ccggcgagat aaaccatctg cacgtcgcgc ccggcgacca
     8761 agccgagttt gtctaaatag agcagcgtcg cgtactcgtc gagcccggcg agaccggcgc
     8821 cgagtttctt gcctttcaaa tcggccggag tacggatcga tgaattgacc atcaagcgca
     8881 ccggcacata acgcgtctcc gtagcgatca ccatgaagtc gaaatctttt gtcgcaatgg
     8941 cgcgcgacac cgcggtgcca ccaccgtaga gcacttgaat gttgccgttg gccaacgccg
     9001 gcatataggc cgacgcgccc tgcatcaaga ccatctccac gtcgagattg tacttggcaa
     9061 agatcccctc acgccgggcg atccatcctg gacaaaacgt cgccgccggt gaagcataag
     9121 cgacgatgag cttggtggca tccgcgtagg cggcgcggaa atccggccgc agcgaaacca
     9181 agagcaaaat gatcaacgct cttttggtca ttttccctcg ctttgaacca cgtccttgtt
     9241 cgatcggcgt cgccgccgtc atggcacact aatatcggaa taccttaatc caagcgcagc
     9301 gtgcgtgtcc atgataacag aaccacagaa tcgagttcgc cagtttgatt ttgatcagtt
     9361 tccgaagatt tcgcgaagga aatacagccg acgagaaagc gctcggccga gtaaacgcaa
     9421 atcgacgaac taccccccgg cacgcccaaa atatgcgctg agtatcttct tgtttaccgt
     9481 tctttcaata cctcctcgag aattgaatag tccacaagag cgttgacatt gacgcgctgc
     9541 ggaatttcac ggcttcgata ggcgctgtcc agatactctt gcacggcctc cacgccaatc
     9601 ttaccgtcca tgggcaagct agggagaaat tctttatagg tttgttccac cacatcaggg
     9661 ctcaatttcc aggtacgctg aatgaagtca ctaaccttgc ctttctcatt tgcgatgtat
     9721 ctcagactat cgaggaaggc gcgaatcatc gccttcactt gaccccgatt tttctcaacc
     9781 tttgtcttga gaacctgcag accggtattt ggatagacgc cgatgctttg cgtcgatgcc
     9841 agttggttga acgctttttg tttcatcaga atattgaacg gtatggagag cgcggcggca
     9901 tcgacggcct tggcgttaag agctgaaaag gcattggctg ctgaaccggt ctgaatataa
     9961 gtgaccgcat caggattgat tccaccttgc ttcaacatcg atcgggtgag gttgtggctt
    10021 tggctgccga tccgtgaaac ggccaccttt ttgccggcca ggacatctat tttggtaatg
    10081 tcgggctgtg aaactatata aaatagcggc gtcttcgtgg tcgccattac gattgtcgca
    10141 ggcaaacctt tcgccgccag aagcaaacca ggaatgaaac tggaattata gtcgatctcg
    10201 cccgctgcca atgccgcgac ttgggccggg ccggacacca ggatcgactc cacgtccaag
    10261 ccatgctttc taaaaatccc cgcgtccttg gcaatcaagt tgacgaggaa agtaaaactg
    10321 accgtcgaca atgcaacccg gattttttca cgatcctggg catgcgccaa accaagaaac
    10381 agttgaagca acaggataag cgcactgatc gattttcgcg tttgcttcga ttttgggttt
    10441 ttcaccgtat gcataccaag ctcctcgatc cacaacc
//
LOCUS       DATLCH010000299         4754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6358210, whole genome shotgun sequence.
ACCESSION   DATLCH010000299 DATLCH010000000
VERSION     DATLCH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6358210"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VMZ02_08830"
     CDS             <1..784
                     /locus_tag="VMZ02_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72078.1"
                     /translation="KQVFLNPDCRASDSCDLKRFTLTHVVNEIWFSDNADHPTYSNGV
                     IMEYETDSVAALEKYAIVQFKKGCVFDTYKNQDGKIRRNVTDTVASFGEKIPFCFHRW
                     VIDSQDRDPAYNSDPEFGRFYLLRWNTPGSYDNRTQKYYGAEKPKTPVVYMADYPAGA
                     FISRTSVRNVALQFNTCIFKASHVPSETNRNNLGFAQPLGCFEWRNVYVYDFAKARFD
                     SDSAALASWEEPSPTENYRRYVIVTVLMVVAFIGYLGWRRLR"
     gene            1159..1467
                     /locus_tag="VMZ02_08835"
     CDS             1159..1467
                     /locus_tag="VMZ02_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72079.1"
                     /translation="MKHFGTRLSMLFLAGSLAGSAMAANNEVSANAAAGETYCNMKFP
                     AVTQKSLASRHPQLKSAQTGDVIDYSGPCDANPTGKDQVQEQKREESFMFGRAYEDGD
                     "
     gene            complement(2421..2636)
                     /locus_tag="VMZ02_08840"
     CDS             complement(2421..2636)
                     /locus_tag="VMZ02_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72080.1"
                     /translation="MAEAILQDHPKLRVILASAFADQALHAYTTAKGIILLSKPFTTD
                     QLLGAVDLALSERGAGTGCPNNHKTRQ"
     gene            complement(2664..2819)
                     /locus_tag="VMZ02_08845"
     CDS             complement(2664..2819)
                     /locus_tag="VMZ02_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72081.1"
                     /translation="MRVLIIDDDEVFGRLLVELLADFGIGAVSITDGIAAYELVTGDA
                     FDLCIID"
     gene            complement(2954..4354)
                     /locus_tag="VMZ02_08850"
     CDS             complement(2954..4354)
                     /locus_tag="VMZ02_08850"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HUR72082.1"
                     /translation="MNAQILIADDDEVSCQLFAETLEGRGYDVQKVTSGDQAIASLAQ
                     RLYDLLIVDVRMPGTSGLEVTRIVHDKYPSLPVIVMTAFGSMETAVEAIHEGAFDFIS
                     KPMNLAELRKTVERALASRSMPGPADPASELNPLLSPRQQASKIIGKTPAMLEVYKIV
                     ARVAPAKSTVLILGESGTGKEMIARAIHEHSPRAHRPFVAVDCGALTESLLESELFGH
                     VRGAFTGAIADKKGVFEDAQGGTCFLDEIGGISSNLQARLLRVLQEHEVRRVGGKDWT
                     SVDARVVAATNFNLPEAVRLGEFRQDLYYRLNVVAIHLPPLRERRDDIPLLAQHFLTH
                     YSEENNKQIPRISDDAMALLCAYAWPGNIRELENAIEQAVALSYRPSLTAEDLPRDIR
                     EPKTSGLSPAPAGGEEFLFPDTPSLEDVKRRYVLHVLRQTGGNISATARLLNIDRRSL
                     YRMFARYKIEAVTKSD"
     gene            complement(4329..>4754)
                     /locus_tag="VMZ02_08855"
     CDS             complement(4329..>4754)
                     /locus_tag="VMZ02_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUR72083.1"
                     /translation="DGSPVIHGDGTSIQRVLINLLDNAIDACDHGGHVEVSSLESAGV
                     GDHGHYVVIKLVDTGVGIPPEMLPKVFDLFVTTKAPDKGTGLGLAICQEIVNAHGGSI
                     GVESRLGEGTTITVSLPSAAAAIATPPIENQDERTDSDR"
BASE COUNT         1124 a         1336 c         1254 g         1040 t
ORIGIN      
        1 caaacaggtc tttttaaatc ctgactgccg cgccagcgac agctgcgatc tcaagcgatt
       61 taccttgacc cacgtggtca atgagatttg gttcagcgac aacgccgatc atccgacata
      121 cagcaacggc gtgatcatgg aatatgagac cgattccgtc gctgctctgg agaaatatgc
      181 catcgtacaa ttcaaaaaag gctgcgtgtt cgacacttac aagaatcaag acggcaagat
      241 ccggcgcaac gtcacggata cggtggcgag tttcggcgaa aagattccct tctgttttca
      301 tcgatgggtc atcgattcac aggaccgcga tccagcctac aatagcgatc ctgagttcgg
      361 caggttttat ctgttgagat ggaatacgcc tggctcctac gataaccgga cgcaaaaata
      421 ttacggcgcc gaaaaaccga agacgcccgt cgtctacatg gcggactacc ctgccggcgc
      481 ctttatctcg cgcaccagcg tcagaaatgt cgcactgcag ttcaacacgt gcatcttcaa
      541 agcatcccat gttccctcgg aaaccaacag aaacaacttg ggatttgccc agccgctcgg
      601 ctgcttcgaa tggcggaacg tttatgtcta cgattttgcc aaggcaagat tcgacagcga
      661 ctcagcggct ctggccagct gggaagagcc gtccccgacc gagaactacc gacgctatgt
      721 cattgtcact gtgttgatgg ttgtggcatt catcggctat ttgggttggc ggagacttcg
      781 ctgacttaag cagctcgtat tgcggtcaaa taatcaccgc tcagattccg agcgcagcaa
      841 tcattccttc accccactcg ttcgggcgac atgcgtttgt cgaagtagta aaaccacgct
      901 ctggtcccgg tcggccaaag ccaccgactt tcttctaatc atcgcgcagc ttgcagttat
      961 ttcgctccta ttgccgtgtg gcatattgct tgctcgccaa tagatccagg atccataaac
     1021 ggaaccggcc cgtgacgaaa gaaggtgatg aaatcatgta tcgcttcgag tcaaagacat
     1081 ggtgatacgt tcaagaaccg ctgactcggt gagtacgccg tgtccacggc ataaccattc
     1141 agaaagggag ttagaatcat gaaacacttc ggaactcggc tgtccatgtt gtttttggcg
     1201 ggctctttgg ccggcagcgc catggcagct aacaatgaag tatcggcaaa tgcagcggcg
     1261 ggcgaaacct actgtaacat gaaattcccg gcggtgaccc aaaagagtct ggcgagcagg
     1321 cacccacaac taaagagcgc gcagacaggc gacgtcatcg actattcggg tccgtgcgac
     1381 gcaaacccga ctgggaagga tcaagttcaa gaacagaaac gcgaagagtc cttcatgttt
     1441 ggacgagcgt acgaagacgg cgattaatcc gtatcgaaag actccagagc gcggcgagaa
     1501 acaatctcgc cgcgcaattc tactcaactt taaacccaat catgcaagga tatccgtcgc
     1561 caatttcgcg ccgagaactg aacgggcgac gcgatgcatg agtcgcgatg ccttttcgcc
     1621 acacgctgac gcagttgcgc ctcagtccgc ctatccctcg ggaacgttgc gagtgcgtcc
     1681 atcgactgca gttgccgacc gctgagatgc tagcgtgatc tgcatgagga actaatagtc
     1741 cgatgtggcg actaccgatc ctgaatttat gtttcctgct gatcctgata accattctta
     1801 cgttgcagca ttcggccgct gtctttgcac cctttgagcg ttcagatggg tccggcgatc
     1861 cgccccttgg cgcccatagg gttcgaggga atgcttcttc ggacaatggc ttgtggactg
     1921 ccctcgatta tttgagtgaa ttggggcaac gttcacggat aatcaaggat tggtatctcg
     1981 agaatcgaac accctcctgg gacaggaggc gcgctgcgcc cggggtcgaa aacgatctgc
     2041 tcgaagcgcg atttcatgtc gccaacgccg cagcgctaag cgccgcgact agcgatcggc
     2101 acaggactac aaccgaactc gaccgagctg acaagtgctt gcaaaaagct atgggaggcg
     2161 ttggcgaaca tctccggccc ttcgttagcg ccgttcgagg cgagttgacg caagccaagc
     2221 gagagttaga taggggcgat ccggacaccg acaccagaga tgaacaaatc aaaatcgatt
     2281 tggatgctgt tctccaggcg ctacacggcc actgaccgac agcgatttga tctgccgcag
     2341 gtgggatcga cgcgcggcag aatcgatcat gcgaaataac aactcatcaa tatcatccgg
     2401 gcgattcgta aaaatccgtg ctactggcgc gttttatggt tgttaggaca accggttccg
     2461 gcgccgcgtt cactcagggc gaggtccacc gctccgagca attgatccgt agtaaatggc
     2521 ttcgataaaa gaatgatgcc cttggccgtg gtatatgcgt gcaatgcctg atccgcaaaa
     2581 gccgaggcta aaataactct tagtttcgga tggtcctgca aaatcgcctc ggccaaatca
     2641 gcgccgagca ctaagggcat gcgttaatcg attatacaga gatcgaaagc atcgccggtc
     2701 accaactcat aagcggcaat accatcggtg atagagacag caccgatacc aaagtccgcg
     2761 agtaattcca ccaacaaccg gccgaacact tcatcgtcat cgataatcaa cactcgcatc
     2821 tcgacgctcc tcccatcgat gatccaagtg caaggttggt gccggacaaa cgttggatgc
     2881 ggtgattgga ttgtgccagt tcgccctggt tagatcagcg cgcggaccga gcacgaggcc
     2941 gatgaatttc ccatcagtca cttttcgtaa ccgcttcgat cttatagcga gcaaacattc
     3001 gataaagcga gcgtcggtca atgttgagca gtcgggcggt cgccgatata tttccgccgg
     3061 tttgtcggag cacatgcagc acatagcgtc ttttcacatc ctcaagactc ggagtgtcgg
     3121 ggaacagaaa ttcttcaccc cccgccggtg caggtgaaag tcccgacgtt tttggctctc
     3181 taatatcccg gggtaaatcc tcggccgtca gtgatgggcg gtaagagagc gcaacagcct
     3241 gctcaatggc gttttcaagt tcgcgaatgt ttccgggcca tgcataggcg caaagaagtg
     3301 ccatggcgtc atcggatatc cgcggaatct gcttgttatt ttcctcgcta tagtgcgtga
     3361 gaaagtgctg cgccagcagt gggatatcgt cgcgccgttc acgcaacggc ggcaagtgaa
     3421 tcgcaacgac gttcaaacgg taataaagat cctggcgaaa ctcaccgagc ctgaccgctt
     3481 caggcaggtt aaaattagtc gcggcgacaa cccgggcatc caccgaagtc caatccttgc
     3541 cgccgacgcg gcggacctca tgttcctgaa gcacccgcag gagccgcgcc tgcaagttcg
     3601 aactgatgcc gccgatttca tcgaggaagc aggtaccgcc ctgggcgtct tcgaacactc
     3661 ccttcttatc ggcaatcgcg ccggtaaatg cgccgcgtac atggccaaaa agctcgctct
     3721 cgagaagtga ttcagtcaac gcaccgcagt ccactgcgac aaagggtcgg tgagcgcgtg
     3781 gactgtgctc gtgaattgcg cgcgcgatca tctccttccc agtgccgctc tctccgagga
     3841 tcagcacggt gcttttcgcc ggtgcgaccc gtgcgacaat cttataaact tccagcattg
     3901 ctggagtctt gccgataatt ttactggctt gttggcgcgg cgataatagc ggattaagtt
     3961 cgctcgccgg atcggcgggg cctggcatgg agcgcgaagc caatgccctt tcgacggttt
     4021 ttcgcagctc cgcgagattc attggtttgg aaatgaaatc gaacgcgccc tcgtgaatcg
     4081 cttccacggc tgtttccatg gagccaaagg cggtcattac gatcaccggt agactgggat
     4141 acttgtcgtg tactatccgc gtaacttcca aacccgacgt accgggcatg cgcacgtcga
     4201 cgatcagcaa gtcatagaga cgctgcgcga gggatgcgat tgcctggtca cccgaagtca
     4261 ccttttgcac atcatagccc cggccctcca aggtctcggc aaagagctga caactcactt
     4321 cgtcatcgtc agcgatcaga atctgtgcgt tcatcttgat tttctattgg cggcgtagcg
     4381 attgccgcag ccgcactcgg cagggacacg gtaatcgtcg ttccctcgcc cagccgactt
     4441 tcaaccccga tggacccgcc atgagcgttg acaatttcct gacagatggc cagacctaga
     4501 ccggttcctt tatctggcgc ctttgttgtg acgaaaagat cgaatacctt gggcaacatt
     4561 tccggcggta tgcccacccc ggtatccact agcttaatga ccacatagtg tccgtgatcc
     4621 ccgacccccg cgctttcaag gctcgaaacc tcgacatgac cgccgtgatc gcaggcatcg
     4681 atggcgttgt ccaataagtt gatcagcact cgttgaatcg acgtaccatc gccgtgaata
     4741 accggcgagc catc
//
LOCUS       DATLCH010000300         4710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3427457, whole genome shotgun sequence.
ACCESSION   DATLCH010000300 DATLCH010000000
VERSION     DATLCH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3427457"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VMZ02_08860"
     CDS             <1..813
                     /locus_tag="VMZ02_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72084.1"
                     /translation="GQVDVISNIPPHEVARLKANPRLRVQPVQGLRPLFLVLSPHYKP
                     LDNVKVRRAMTHAIDRERIIKHILEGNAYPLSGMLSPQVFGYDPGAKAFAYDPEKAKQ
                     LLVEAGFPNGFEIDYFSPTGRYPKDKEVALVIVEQLARVGIKANLKTPEWAIFNTDYK
                     KGKYPMYLTGRGSLTDADALFQQYFRSGVTPRVLGYSNPKLDEILDLEAQTFDSKKRE
                     KLLQDAHRIILDDAPAIPLWNAMDIYAHRADLIWSAPPDEKVQLKQASFKTK"
     gene            complement(835..1260)
                     /locus_tag="VMZ02_08865"
     CDS             complement(835..1260)
                     /locus_tag="VMZ02_08865"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelL-related redox protein"
                     /protein_id="HUR72085.1"
                     /translation="MPCQAHLVELRQQHAEFARRGVEIAVISFAEPARLVRYQEHHQW
                     PFLVLADPARKAYQVFALKRLSWFQVFSLPALKVYWKLLRGGMRPEHYDGDDIYQSGG
                     DFLIDANGNLLFAHRSEDPADRPAAARLLTAIDRVQRER"
     gene            complement(1562..1906)
                     /locus_tag="VMZ02_08870"
     CDS             complement(1562..1906)
                     /locus_tag="VMZ02_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUR72086.1"
                     /translation="MSSKIYVGGLPYATTDAQLQDLFSAHGAVESARVITDKFTGRSR
                     GFGFVEMSNSDEAQKAIQALNGTDFEGRNLTVNEARPQERRPGGGGGFGDRGGERRGG
                     GGGGGGGGRNRW"
     gene            2244..2999
                     /locus_tag="VMZ02_08875"
     CDS             2244..2999
                     /locus_tag="VMZ02_08875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR72087.1"
                     /translation="MYQTNQYEGMLAETVAMQGHKGETINAYMARPLGAGPFPGMVLI
                     HHAPGWDEWYRECTRRFAHHGYVTISPNLYFRDGHGTAEDVGAKVRAAGGIPDEQVLG
                     DLDGANKLLRSQPYLNGKVGVFGTCSGGRHGFLAACRSKGIDALVECWGGGVVMKQEE
                     LTPKKPVAPIDYTKDLSCPMIGIFGNDDKSPSPEQVNQHEAELKKHGKNIEFHRYDGA
                     GHGFFYYDRPAYRQEQAVDGWKKVLAFCEKNLK"
     gene            3017..3307
                     /locus_tag="VMZ02_08880"
     CDS             3017..3307
                     /locus_tag="VMZ02_08880"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HUR72088.1"
                     /translation="MCTSITHQAKIYGRGKQGEEWIDLKECNVSYDHPYGMPLEHALN
                     IDFVDESKGPGARVAVELSVEAARQLVKTIEAVLAQAEHQGVLEDKHQAAIG"
     gene            3431..4678
                     /locus_tag="VMZ02_08885"
     CDS             3431..4678
                     /locus_tag="VMZ02_08885"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72089.1"
                     /translation="MSNSEDISASAAAPTRPWASLTIRDYRLMWIASVCNAVAIQVRN
                     VAGLYQVYEISGSALQLGLTGFFQAFPFVIFGLFAGAVADAFDRKKLLVVTVAIQLIP
                     SLLLGFLTMTGTIQVWHIFSLGFVGALVEVFNWPARSALIPRLVPPSYMMNAVTLNTI
                     IIQTSFLLGPALGGVLIDQSGLVSTYFLTIAMFIPSGIAIFAMRTSGVPEGERRRVNL
                     KSIFEGIEFIWVQRIILSLFLLDFGVTLVGFYKPILPIFAADVFKTGASGLGILYGAP
                     SLGAIVGSIVLLMIGNVKRMGVLVVGAAVVFAASLALLGVSNWFWMAVGTTLILGFAD
                     SVSVAIRRTVVQMLAPDGMLGRASSLITAFAQATNGLGAVLAGFAAQQVGAPNALLLG
                     SALCLVMILGICLAIPQLWRYRS"
BASE COUNT         1035 a         1373 c         1283 g         1019 t
ORIGIN      
        1 ggccaggtcg acgtcatcag caacatcccg ccgcatgaag tcgcacgttt gaaagccaat
       61 ccgcggctgc gcgttcaacc ggtgcaagga ctgcggccgc tctttctcgt gctgagcccc
      121 cattacaaac cgctcgacaa cgtcaaagtg cgccgcgcta tgactcatgc gatcgatcgc
      181 gagcggatca tcaaacatat actcgaaggc aacgcttacc cgttaagcgg catgctcagc
      241 cctcaggtat tcggctatga tccaggcgcc aaggctttcg cttacgatcc ggaaaaagcc
      301 aagcagttgc ttgtcgaagc ggggtttccg aacggattcg agatcgatta tttcagcccc
      361 acgggaagat atcccaagga caaggaggta gccctggtga tcgtcgagca gcttgcccgc
      421 gtcggcatca aagccaatct caagacgccg gaatgggcga tcttcaacac cgactacaaa
      481 aaaggaaagt atcccatgta cctgaccggc cgcggcagcc tcaccgacgc ggatgcgctg
      541 tttcaacagt actttagaag cggcgtcact ccccgggtgc tcggctacag caatcccaag
      601 ctcgacgaga tcctcgatct cgaggcacaa accttcgaca gcaagaaacg cgagaaactg
      661 ctgcaagacg cgcatcgaat aatcctcgat gacgcgccgg cgattccatt atggaacgcc
      721 atggatattt atgcccaccg ggccgatctt atctggtccg ctccgccgga cgaaaaggtt
      781 caattgaagc aagccagctt caaaacgaaa tagccgatgc gctgttctca gggtttaccg
      841 ctcgcgttga acgcgatcga tggccgtgag cagccgcgcc gccgccggcc gatccgcggg
      901 atcttcgctg cgatgggcga acaataggtt tccattggca tcgatcagga agtcgccgcc
      961 ggactgatag atgtcatcgc cgtcgtagtg ctccggtctc atgccgccgc gcaatagttt
     1021 ccagtacact ttgagagccg gcagcgaaaa cacttggaac caagacagcc gtttcaaagc
     1081 gaaaacctga taggctttgc gcgcaggatc cgcgagaacc aggaacggcc attgatgatg
     1141 ctcttgataa cgcaccagcc gggccggctc ggcaaaggag attaccgcga tctcgacgcc
     1201 gcgccgagcg aactcggcgt gctgctggcg cagctcaacg agatgcgctt ggcagggcag
     1261 tcaagccagg tggcggagaa aaattaacag gagatacttg tcgaactgac cggcgagatc
     1321 gatgggcgcg ccatggagat ccactagcgg caattgtgcg atgtcgatct tatccacgga
     1381 gatggggcgg gcgaatacag acagttgccg ctaattcaga ttccaatttg cgaactttgc
     1441 gttctttgcg gctcaacgtt ctggtcgcgt gcattttcgg cggcgccaaa aaaacatcac
     1501 caaataaaaa gggcgccccg acctagcgcc cttctcgatg aatgaaaata gtgactgaag
     1561 actaccagcg attgcgtccc ccgcctcctc cgccaccacc accgccacgg cgttcgccgc
     1621 cgcggtcacc gaagccaccg ccgccaccgg gacgacgttc ctgaggacgc gcttcattga
     1681 cggtcaggtt gcggccttcg aagtcggtgc cattgagcgc ttggatagct ttttgcgctt
     1741 catcggaatt cgacatctcg acgaaaccga aaccccgcga acgtccggtg aacttatcgg
     1801 tgattaccct tgccgactcc accgcgccgt gggccgaaaa cagatcctgt agctgtgcat
     1861 ccgtggtcgc gtacggcaaa ccacccacgt aaattttact gctcatgctc tctccttcta
     1921 aacatctgtt atggtctttg ccctgagaac tgattaccgc tggcatcaga acatggaatg
     1981 agagtcacgc tctggtcgga ttgtccgcgt ggcaccgaac tgcaaggtaa gacgccctag
     2041 accttcaact ttgcttttat tcgtcactat cagaaggccc catactgatg tgtaaaaaaa
     2101 gcttaactta agagcccagc cgcgtgcaag accagaatta aggaaatttt ccgccgagga
     2161 aagacctcat ttggttgcag gaatgggccc tttcgtacta atgaattctg atcttatttg
     2221 aagcttttaa ggaggagcag cagatgtatc aaaccaatca gtacgaggga atgttagccg
     2281 aaaccgtggc tatgcaaggc cataaaggcg aaaccattaa tgcctatatg gcccgtccgc
     2341 tcggcgccgg gccgtttccc gggatggtgc tgatccatca tgcgcccggc tgggacgaat
     2401 ggtatcgcga atgcacacgt cgcttcgccc atcatggcta cgttaccatt tcaccgaatc
     2461 tatatttccg cgacggccac ggtaccgctg aagacgtcgg cgccaaggtg cgcgccgccg
     2521 gcggtatccc tgacgagcag gtgttaggcg atctcgatgg cgccaataag ctgctgcgct
     2581 cgcagcctta cctaaatggc aaggtcggcg tcttcggtac ctgctccggt gggcgccatg
     2641 gcttcctcgc tgcctgtcgc tccaaaggca tcgacgcgtt ggtcgaatgc tggggcggcg
     2701 gcgtggtcat gaagcaagaa gaactaacgc cgaaaaaacc tgtggcgccc atcgactaca
     2761 ccaaggatct ctcctgcccg atgatcggta tcttcggcaa cgacgacaaa tcgccgtcac
     2821 ccgagcaggt caatcaacat gaggcggagc tgaaaaagca cggcaagaac atcgaattcc
     2881 atcgctacga tggcgccggc catggtttct tttattacga tcgacccgct tatcgccagg
     2941 agcaggcggt cgacggttgg aaaaaggtcc tcgccttctg cgagaaaaac ttgaaatagc
     3001 cagcggaggt ccgatcatgt gcacaagcat cacccatcag gcaaaaatct atggccgcgg
     3061 caagcaaggc gaggaatgga tcgatctcaa agagtgcaat gtttcctatg atcatcctta
     3121 cggcatgccg ctggagcatg cgctcaacat cgactttgtc gacgaatcga aaggccccgg
     3181 cgcccgagtc gcagtcgaat tgagcgtcga agcggcgcgc caattggtaa aaaccatcga
     3241 agccgtactc gcgcaagccg agcaccaagg cgttttggaa gacaagcacc aagctgcgat
     3301 cggctagctt ggcaataaag tctcaggcgg cgaagtggat gcaccacttc gccgcctcac
     3361 tcacccgtcg caatccgctg ccgctcacgt tatagctgtc ggtacgtttt cgtttgaatc
     3421 gctcgtcccc ttgtctaatt cagaagatat atctgccagc gccgccgcgc cgacgcgtcc
     3481 ctgggcgtca ttgacgatcc gtgattatcg tctgatgtgg atcgccagcg tctgcaatgc
     3541 ggtcgccatc caagtgcgta acgtggccgg tctctatcag gtctacgaaa tttccggctc
     3601 ggcattgcaa ttgggcctca ccggtttttt ccaagcgttc ccgttcgtca tattcggact
     3661 gttcgccggc gcagtggccg atgctttcga tcgcaagaaa cttttggtcg taacggtggc
     3721 gattcaactg attccaagtc tgctgcttgg ctttttgaca atgacaggga caattcaggt
     3781 ctggcatatt ttcagcttgg gttttgtcgg tgcactggtg gaagtattca attggccggc
     3841 acgttcggca ttgattcccc gcctggtacc gccgtcttat atgatgaacg ccgtgacgct
     3901 caacacgatc atcatccaaa ccagctttct cctcgggccg gcgctgggcg gcgtgctgat
     3961 cgaccaaagc ggtctggtca gcacttattt tttaaccatc gccatgttca tcccttcggg
     4021 catagcgatc tttgcaatgc gcacctccgg tgtccccgaa ggcgagcgcc ggcgcgtcaa
     4081 tctgaaaagc atttttgaag gcatcgagtt tatctgggtg caaagaatta ttttatcgct
     4141 gttcctgctc gactttggcg ttactctagt aggcttttac aagcccatcc tgccgatctt
     4201 cgccgccgat gtattcaaga ccggcgcttc cggattgggc atcctttacg gcgcgccgtc
     4261 gctcggggcc atcgtcggtt cgatcgttct gctgatgatc ggtaatgtca aaagaatggg
     4321 cgtgctggtc gtcggcgccg cagttgtctt cgccgccagc ctggcccttc tcggtgtgtc
     4381 caattggttt tggatggcgg tcggtacgac gcttatcctc ggtttcgccg actcggtgag
     4441 cgtggcgatc cgtcgtacgg tggtacaaat gctcgcgccg gatggcatgc tcggtcgagc
     4501 gtcaagtctg atcaccgcct tcgctcaagc caccaatggc ctgggcgccg tcctcgccgg
     4561 cttcgccgcc caacaagtcg gcgcgcccaa tgcgctgttg ctcggcagcg cgctctgcct
     4621 ggtgatgatc ctgggtatct gcctcgccat tccgcagctg tggcgctatc ggtcgtagaa
     4681 acagatcgac cgcaaagaac ccaaagctcg
//
LOCUS       DATLCH010000301         3258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7850902, whole genome shotgun sequence.
ACCESSION   DATLCH010000301 DATLCH010000000
VERSION     DATLCH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7850902"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VMZ02_08890"
     CDS             <1..500
                     /locus_tag="VMZ02_08890"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HUR72090.1"
                     /translation="IAQHGGALTHLSVYEPARAPITSFAVQVAEVTVEPATGQVKVNK
                     LTTVHDSGTVLNHLSYTGQIDGGIINGLGFALMEDNSLIDGTMPTVNLGEFKMANIAD
                     LPKLTTVLMESPAGPIPYQGKAIAEIPNVPTAAAIANAVQDACSVRILDLPITAEKVY
                     GALKN"
     gene            complement(891..2468)
                     /locus_tag="VMZ02_08895"
     CDS             complement(891..2468)
                     /locus_tag="VMZ02_08895"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR72091.1"
                     /translation="MKASFRDYIDTLRNNDELLEIAKPVDLRDVAALVAQSEKALLFK
                     NLPGYSMSLVSGLLQSRKRIALAMGVDYANIADKLGKAMDKPIEPERVADAPVKEVRI
                     TGKEVNLFDLPVPVFSVMDGGPMITGGVVIAEDPEFGINAGIYRLMLKERNITGIDIV
                     TPNNMRRFAERALAQKKPLPISISIGTHPYELVASTFKANLGVNELTFAGGLRNEPVR
                     LADGETVAVPCIADAEIVLEGEILPEGWVHTEGPFSEFNRLMGGMHMNPRVRINAIMH
                     RKDAIYYALQMPWENIWMSAPIYEAAARRVLFEAGVQTIAINVTPGGCCHWHIIASIK
                     KVPGDGKNAIMALLSIADIKHVTVVDDDIDVFDPVDVEWAVATRVQADRDVVIVSNAR
                     SKPLDPSLPLPLHGKVPTTAKMGIDATIPENIPAERYRRIVYFNQDKVKLSDYVGVAE
                     STDKGRTKPVETVESLSQRISAALGKSHCFFADLLEMFPQADYKIIANAVARLYEDGK
                     IKQDKDGKFELTQSAEA"
     gene            2707..>3258
                     /locus_tag="VMZ02_08900"
     CDS             2707..>3258
                     /locus_tag="VMZ02_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR72092.1"
                     /translation="MATFGLTLANRGVIIGAVTVPELFDMAKRGEDSGAFSAVWVGDS
                     LLAKPRLESITLLSALAVVTKKVKLAVGCMATFPHRHPALLAQQWASLDVISGGRSWL
                     AVCLGGPNEQSPAQALEHKVMGIKDTERVGRLEEGIVILRKLFHEEKASHKGRFYEFE
                     GVTIQPHPVQNPLPIWIASNPTGL"
BASE COUNT          734 a          925 c          874 g          725 t
ORIGIN      
        1 ccatcgcgca gcatggcgga gcgctgactc atttgagcgt ctacgaaccc gcgcgcgcgc
       61 cgatcacttc cttcgctgtg caagttgccg aagtcacagt cgagcccgct accggccaag
      121 tcaaggttaa caaactcacc accgtccatg attccggcac ggtgttaaac cacctgagtt
      181 acaccggtca aatcgatggc ggcatcatca atggcttggg cttcgcgctg atggaagata
      241 attcgctaat cgacggcacg atgccgacag tgaacctggg tgaattcaaa atggccaata
      301 tcgccgacct gccaaaattg actacggtct tgatggagag tcccgccggc ccaattccct
      361 atcaaggcaa agccatcgct gaaatcccca acgtgccgac agcggcggcc atcgctaacg
      421 cagtgcaaga cgcctgcagc gtgcggatat tagatttgcc gataacggcg gaaaaggttt
      481 acggtgcgct gaagaattga ggactggaac gtttagccgc atacgacgtc aaaaacaatt
      541 gcgacttgta gggcctgtga ttggccttct tatctaaaac aagaccgtcg acgaaatact
      601 gctccagccg cgctatatac cgcgcgggat aggccaaaac cagcgccctt cctcggatca
      661 tttgggtagg cacaatttct taggttctac agcagttaaa agccacgctg atcttcccat
      721 cgtagtgact gattcagtca tccgtcatgc cggtgcaggc cggcatccat cagaacgtat
      781 aaccaaccgc aagattcgcc tggattcagc ctgcgccgga atgacaaatc gaagctcgtt
      841 cagttttgta gacctcagca gacatctagg aaagataata gcgtgtggct ctatgcctca
      901 gcgctttggg ttaactcgaa cttgccgtcc ttatcttgtt tgatctttcc gtcctcgtac
      961 aagcgcgcaa ccgcattggc tatgatttta taatccgcct gcgggaacat ttctaaaaga
     1021 tcggcaaaaa agcagtgcga cttgccgagc gcggcagaga tcctttggct caggctttca
     1081 actgtttcca ccggtttggt tctgcctttg tcagttgatt ccgcgacgcc gacatagtcg
     1141 ctcagtttga ctttatcttg attgaaataa acgatccgtc tgtagcgttc cgccggaatg
     1201 ttttcgggaa tcgtcgcatc gatgcccatc tttgcggtgg tcggcacttt gccgtggagc
     1261 ggcagcggca gcgacggatc gaggggcttc gatctggcat tggagacaat aaccacgtcg
     1321 cggtcggcct ggacccgggt ggcgaccgcc cactcgacgt cgactggatc gaatacatcg
     1381 atatcatcgt cgacgacagt cacgtgcttg atatcggcga tggacaagag cgccataatc
     1441 gcattcttac cgtcacccgg tactttcttg atcgatgcaa tgatatgcca atggcagcag
     1501 ccgccgggag tgacgttgat ggcaatggtc tgaacgccgg cttcgaaaag cacgcgccga
     1561 gcggccgcct cgtagatcgg cgccgacatc cagatatttt cccagggcat ttgcagcgcg
     1621 taatagatcg cgtccttacg gtgcatgatt gcattgatgc gcacccgcgg attcatatgc
     1681 atgccgccca tgagccggtt gaactctgag aacggccctt ctgtatgcac ccaaccctcc
     1741 ggcaaaattt ctccctcgag aacgatctcg gcatcggcga tgcacggcac ggccacggtc
     1801 tcgccatcgg ccaagcgcac cggttcattg cgtagcccgc cggcaaatgt caattcattg
     1861 acgccgagat ttgccttgaa ggtcgatgcg acaagttcgt acgggtgtgt gccgatggat
     1921 atcgaaatcg gtagcggttt cttctgcgcc agagcgcgct cggcgaaccg gcgcatattg
     1981 ttcggtgtaa cgatgtcgat gccggtaatg ttgcgttctt tgagcatcaa acggtaaatg
     2041 ccggcattga tgccaaactc cgggtcttcg gcgatgacca caccgccagt aatcattggc
     2101 ccgccatcca tcaccgagaa caccggaacc ggcagatcga acaggttcac ctccttgccg
     2161 gtgattctaa cttccttgac cggagcgtcg gcgacacgct caggctcgat cggcttatcc
     2221 atcgcctttc ccaacttatc ggcgatgttt gcatagtcga cccccatggc cagggcgatg
     2281 cgcttgcgcg actgcaacag accggaaacc agcgacatag agtagccagg caggtttttg
     2341 aacagcagag ctttttctga ctgcgcgacc aaggcagcga catcgcggag atcgaccggt
     2401 ttggcgattt ccaaaagttc gtcgttatta cgcagtgtat cgatgtaatc gcgaaagctc
     2461 gccttcatgg atggactcca agcggtttga ttcgaggaga cttagcacgg cgatactgcg
     2521 aattccatcg aagagtaagg cgccacgagc tgtctcgaac aagcgcagat cacgcagcaa
     2581 atttccaatt cgattccaca cggcgtcttc cgctctctgc cgtgccacag tcccttcgta
     2641 tttcgcaaag ctatcgctat gtgatataaa cgcgggcgaa cccacgaact tttgggaagg
     2701 caatcaatgg ctaccttcgg acttactttg gcaaatcgcg gcgtgattat cggcgcggtt
     2761 accgtaccgg aactcttcga tatggcgaaa cggggcgaag actccggcgc cttcagcgct
     2821 gtgtgggtcg gcgacagttt gctcgccaag ccccgcttgg agtcaatcac tctactttca
     2881 gctctcgcgg tggtcacgaa gaaagtaaag ctcgcggtcg gctgcatggc gaccttccca
     2941 catcgccacc cggcgctttt ggcgcagcag tgggcgagct tggacgtgat ctccggcggc
     3001 cgctcctggt tggcggtctg cctcggcggt cccaatgagc aaagcccggc tcaagctctc
     3061 gaacacaaag tcatgggtat taaggacact gaacgtgtcg gccggctcga agagggcatc
     3121 gtgattttgc gaaaactatt tcacgaagaa aaagcctcgc acaaagggcg cttttatgaa
     3181 ttcgaagggg tgacgattca gccgcaccca gtgcaaaacc cgctgcccat ctggatcgcc
     3241 agcaatccca cggggctt
//
LOCUS       DATLCH010000302         3924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10098892, whole genome shotgun sequence.
ACCESSION   DATLCH010000302 DATLCH010000000
VERSION     DATLCH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10098892"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VMZ02_08905"
     CDS             <1..829
                     /locus_tag="VMZ02_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR72093.1"
                     /translation="SLDVTITAVRANPPIDITVPSNVRSEPVTVKSDKIVDGVWFIGG
                     GSHNSVLIEMKDYLIVVEAPLDDVRSTAVMGEVKKLVGNKPIKYLVNTHHHIDHSGGV
                     RAYAAEGVTIVTHELSRPYFDRATANSWTLSPDRLAKAKKKPVFQTMGDNMVLTDGTR
                     SVELYQIVGNGHHDGLVMAYLRKEKLLVEADAFSPFGVPKTPNPYSVNLEANVRRLNI
                     DVDKILPLHGEVVAYAELMKAIGKGPGDKAAEKAPEKPPAKTPEKAPEKAPEKTPAK"
     gene            972..2417
                     /locus_tag="VMZ02_08910"
     CDS             972..2417
                     /locus_tag="VMZ02_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR72094.1"
                     /translation="MFSKLLTALVLATLMGLRAEAQESDLQRIADMLDVSSTKTFQFT
                     ANGSMYFLGQSTSPAAPWPLQFVKSMTRVYDFTAGAMRDSVVRMTADSLPIGPEQQSV
                     ALVSGDLAWNVAGKETPARLFEAAERAHQIVISPHGLMRAAFANNAAVTKKSIDGREM
                     TIISFTVRGNHKVVAYANDQNAIERVDSAYGSPVVGDMNVVTYYGTYRDFGGIKFPSK
                     IIQYQDGKPSLDVTVTAVSANPPVDIEVPANVRDNPVAAKSEKIADGVWYITAPANNN
                     SVLIEMKDYLIVVEAPHGEQRSLAVMGEVKKLVPNKPIKYVINTHHHFDHSSGLRAYA
                     AEGVTIVTHEINRPYFERAALNSWSLGPDRLARSKKKPVFQSMGDNMVLTDGTRSVEL
                     YQVVGNVHHDGIVMAYLRKEKLLIEADVFTPGPVGAEPPKVPSPSAVNLEANVRRLNI
                     DVDRIVSIHQRVVPYSDLLTAIGKKPAPPAK"
     gene            2808..3839
                     /locus_tag="VMZ02_08915"
     CDS             2808..3839
                     /locus_tag="VMZ02_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR72095.1"
                     /translation="MFNRILLSLGLFLTSALDAGAQAPFYQNKTVTIVVGYLAGDGYD
                     VWARLLAAHMGKHIPGNPNFVVVNTPGAGSMIAANNIYNVAKPDGLTMAAIGPSLYLD
                     QVIGKKEVKFDWSKFGWVGSTENTPWLFYMSTTTPFKSIEDVRKAKEAPKCSATSSGT
                     SGHFIPKLLEEAFGAKFTVVMGYQGGSEQDLAFERGEVVCRALSIPTFFAREPFTTWR
                     KKNMVRILLQTGRKRDPKMPDVPTIHELMTEHKTPPPTRALVTAILASGDLGRPFITP
                     PAIPADRLKILREAFRKTMADPGFLADVKARKFEIDPDYGEELEALAKEIVAQPKEIV
                     ERMKKLLSE"
BASE COUNT          911 a         1068 c         1115 g          830 t
ORIGIN      
        1 atcgctcgat gtcaccatta cagccgtaag agccaatccg ccgatcgata tcaccgtgcc
       61 gagcaatgta cgcagcgagc cggtgacggt gaaatcggat aaaatcgtcg acggagtttg
      121 gtttatcggc ggcgggtcgc ataacagcgt gctcatcgag atgaaagatt atctgatcgt
      181 cgtcgaagcg ccgttggacg atgtccgttc aaccgcggtg atgggcgaag tcaaaaaact
      241 ggttggcaat aagccgatca aatatttagt caacacgcat caccacatcg atcattcggg
      301 tggagtccgc gcgtacgcgg ctgaaggcgt cacgatcgtc acccatgaac tgagccggcc
      361 ttatttcgat agggcaaccg ctaacagctg gaccctgagc ccggatcgat tggccaaagc
      421 caagaaaaag cccgtgttcc agaccatggg cgataacatg gtgttgaccg acgggacgcg
      481 ctccgtggag ctctaccaga ttgtcggcaa tggccatcac gacggattgg ttatggctta
      541 tctccgcaag gagaagctgt tggtcgaggc cgacgcgttt tcgcctttcg gcgtcccaaa
      601 aacacctaat ccttattcgg ttaacttgga ggctaatgtt cggcgcctca atatcgatgt
      661 cgacaagatt ctgccgctcc atggcgaggt cgtagcttac gccgagctaa tgaaagcgat
      721 tggcaagggg ccgggcgaca aggcggccga aaaagcaccc gaaaagccgc cagcaaaaac
      781 ccccgaaaag gcgccggaaa aggcaccgga aaaaaccccg gcgaaataat atttcattgt
      841 tccggaaaaa acaggcgcgc agcgcggcaa gagtcgcact cgcgcctttt tttgtcttct
      901 atgttgacca tctctcgcgt aataaattgt ggggctcgta acaaaagact cgtagattct
      961 aggaggctat tatgttcagc aaacttctaa cggcgcttgt actagccacg ctgatgggtc
     1021 ttcgcgccga ggctcaggag agcgatcttc agcgcatcgc ggatatgctc gacgtctcga
     1081 gtaccaagac gtttcagttc accgccaatg gttccatgta tttccttggc cagagcacca
     1141 gtccggcggc accctggccg ctgcagttcg tcaaaagcat gacacgagtt tacgatttca
     1201 cggccggagc catgcgcgac agcgtcgtgc gcatgacggc cgacagcttg ccgatcggtc
     1261 cggagcagca atctgtcgcg ttggtcagcg gcgacctggc ctggaacgtg gcgggaaaag
     1321 aaacccctgc gaggttgttt gaggccgccg agcgcgcgca tcagatcgtt atctcaccgc
     1381 atggacttat gcgcgctgcg tttgccaaca atgccgctgt caccaagaag tccatagacg
     1441 gccgcgagat gacgataatt tcatttaccg tccgcggcaa ccataaggtg gttgcctacg
     1501 ccaatgatca gaacgcgatc gaacgggtgg attccgccta cgggagtccg gtggtcggcg
     1561 atatgaacgt agtgacctac tacggtacct accgcgactt tggcggcatc aagtttccgt
     1621 ccaagatcat ccaatatcag gatggcaagc cgtcattgga tgtgacggtc accgccgtca
     1681 gtgccaatcc gccggtcgat atcgaagttc cagcgaatgt gcgcgacaac cctgtggctg
     1741 cgaaatccga gaagatcgct gacggggttt ggtatatcac cgctcctgcc aacaacaaca
     1801 gcgtgctgat cgagatgaag gattatttga tcgtcgtcga ggcgccgcat ggtgagcagc
     1861 gctcgctggc ggtgatgggt gaggtcaaaa agctcgtgcc caacaagccg atcaagtatg
     1921 tgatcaacac acatcatcac ttcgaccact cctctggcct gcgcgcttac gcggccgaag
     1981 gtgtcactat cgtgacccat gagatcaacc gtccgtactt cgaacgggcc gcgttaaatt
     2041 cctggagcct cgggcccgat cgcctagccc ggtcgaagaa gaagccggtg ttccagagca
     2101 tgggcgacaa catggtactg accgacggca cgcgctccgt ggaactctac caggtcgtcg
     2161 gtaacgtgca tcacgacggg atcgtcatgg cttacttgcg caaagagaaa ttgctgatcg
     2221 aagccgatgt ctttacgccg ggtccggtgg gcgccgagcc gcccaaagtt ccctcccctt
     2281 cggcggttaa tttagaagcc aacgtgcgcc gcctgaacat cgacgtcgat cgcatcgtgt
     2341 cgatccatca gcgcgtcgtt ccgtacagcg atctgctaac tgccatcggc aagaagccgg
     2401 cgccgccggc gaagtagaca gagcggaaat ggagtagccg aattgcagta atggagtgat
     2461 ggattctgga ttccattact ccgtcgctcc agcactccaa tcaacctcag tgttgacgct
     2521 gcagttattc taagatagcc gtgagctcgt cacgttgacc atcgcggctt gagctcctgg
     2581 cgggcggcgc aacccaggat tcccgccgcg tgtaaattct tcacgaattt gctggatcag
     2641 aaaaacgtgg ctgggaatgc tccgtcaccg gggcagtgat gacgagctgc gcgaaagaac
     2701 taaccagcgc gccgcccgat gtcatcgggc gttgaaaaat tactggcgga tgagccagat
     2761 aaccgtaatt ttacggcgtg tgttcaaaag attcggagta cacatctatg ttcaacagga
     2821 ttcttctgag tctgggatta tttctaacga gcgcgcttga cgccggtgca caggcgccgt
     2881 tctaccaaaa taagaccgtg acgatcgtcg tcggttatct ggctggcgat ggttatgatg
     2941 tttgggcgcg gctgttggcc gcacacatgg gcaagcatat tcccggcaat cccaacttcg
     3001 tggtcgtcaa tacaccggga gccggttcga tgattgccgc gaacaatatt tataatgttg
     3061 ccaaaccgga cggtctgacg atggctgcca tcggaccgtc gctttatctc gatcaggtga
     3121 ttggcaagaa agaggtcaag ttcgattgga gcaagtttgg ctgggtcggt tccacggaga
     3181 atacgccgtg gctgttttat atgagcacga cgacgccgtt caagagcatt gaagatgtgc
     3241 gcaaggccaa ggaagcgccc aaatgctcgg cgaccagtag tggcaccagt ggtcatttca
     3301 ttcccaagct tctcgaagag gcctttggcg ccaagttcac cgtggtcatg ggctaccagg
     3361 gcggctcaga gcaggatttg gccttcgaac gaggcgaagt ggtatgccgg gcattgagca
     3421 ttccgacttt ttttgcgcgc gagccgttta ccacctggcg caagaaaaat atggtgcgta
     3481 ttctgctgca gaccggccgc aagcgcgacc cgaagatgcc cgacgtgccg acgattcatg
     3541 agctgatgac ggaacacaaa accccgccac cgacccgggc tctggtcacg gcgatccttg
     3601 cttcaggcga tctcggccgg cccttcatca cgccgccggc gattccggct gaccgattga
     3661 agattctccg cgaagcattt cgcaagacca tggccgaccc gggttttttg gccgatgtga
     3721 aagcgcgtaa atttgaaata gatcccgact atggcgagga gttggaggca ttagcaaaag
     3781 aaatagtcgc gcaaccgaag gagattgttg agcgcatgaa gaaacttctt tccgagtaat
     3841 tcggcagaga gttccgcgac tgatgttctt tgcgacaatt aacccaccta agtcattttt
     3901 gtctcgcgcc gccacgtccg agcg
//
LOCUS       DATLCH010000303         6677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7859052, whole genome shotgun sequence.
ACCESSION   DATLCH010000303 DATLCH010000000
VERSION     DATLCH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7859052"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..948
                     /locus_tag="VMZ02_08920"
     CDS             229..948
                     /locus_tag="VMZ02_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HUR72096.1"
                     /translation="MNFPIKKSKTKTHAVENLSARVYNQIKQLILCNEIMPGQKLHHQ
                     QLSERLGVSRTPVREALTRLVQEGYVSFLPNRGFTCKEIRLQEAEELYELRESLEAFA
                     VEKAVENSTDQGLAELRQRMESYGHDVYNRFTRDRLVYDQDVHLHIAQIAGNETLKKT
                     LSHVFERIVLKRRTDGIYDAARGLTAHQEHVQLLDAMTRRDAKAAVSIVRHHIQAGKQ
                     NVIADLRQRQAIRELRPADLI"
     gene            982..2352
                     /locus_tag="VMZ02_08925"
     CDS             982..2352
                     /locus_tag="VMZ02_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HUR72097.1"
                     /translation="MAADLVIKGGWVVTPDATFKGGVAISNEVIVAIGADDSLPDGKE
                     VIDVKGKHILPGLFDGHVHFREPGMTHKEDFNSGSTAAVCGGITTVVDMPNTVPPTAD
                     AEQVKVKKELGEAKSLVDFGITGVVVQTNTKDILPMAAAGAIGYKIFFGETIGNLPFP
                     DDGMCVEAFDYIAQSKLPLGIHAENRQIMAYCTNKLKTEGKNDPRYWEASRPDICEAE
                     SVHHAIFFAETFGTKLHVFHMSSKQAAYMVRDAKARGMRITAETGPHYLLREPNDMDK
                     VGSLLKMNPPVRTKDHAEVLWQGLREGFVDMLATDHSPHTLDEKGSDIYGKLTKPAIW
                     DCISGFCGVETGVPLILTEVNKGRLTLNQYVKVASENPAKVWQVYPKKGAIRLGSDGD
                     ITVVDMDKEGTIDVNKLHSKNKPSPWHGWKVKGMPVLTVVRGHVQMKDGEPCGKAIGK
                     ALKPII"
     gene            2502..3413
                     /locus_tag="VMZ02_08930"
     CDS             2502..3413
                     /locus_tag="VMZ02_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HUR72098.1"
                     /translation="MKRTEAFEMFQPATLEEASRLLKEKGTGGRFLAGGTDLVIAMKE
                     KGLLPKYIVDLKRLPGLGGIRENSDGSISLGALTTLHEIETSALIKSKYPFLAQSAAE
                     VGSIQIRNRATIGGNMANASPSADTSPALIALGASVTIASGSGQRQIAAEDFFRGPGQ
                     NAMNSDEILTEVNIPKTGANLVGEYIKFSPREMMDLAYVGVAVVYNLARSDRRCTGVR
                     IVLGAVAPTPIRAKRAEAALESQVLSEAVAEKAGQIAAEEAKPISDVRSSADYRRAMV
                     GTMTKRALLNAAVGAAKSWVERRDRRY"
     gene            3515..3988
                     /locus_tag="VMZ02_08935"
     CDS             3515..3988
                     /locus_tag="VMZ02_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUR72099.1"
                     /translation="MNKQIEFTLNGKSVSREVPTHRLLLDLLRDEIGMTGTKEGCGTG
                     DCGACTVLLDGKPVNSCLVLSGELDGADIVTIEGLRIGPELHPVQQAFVQDGGAQCGY
                     CTPGMLMMSKALLDENPNPTDEEIRYALSGNLCRCTGYAKIVEAVKDAATIIRNG"
     gene            4135..6447
                     /locus_tag="VMZ02_08940"
     CDS             4135..6447
                     /locus_tag="VMZ02_08940"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HUR72100.1"
                     /translation="MAELTVIGKPVTRVDANEKVTGQIIYGYDLALPNMLYGKTLFSP
                     KAHAKIKKIDTTKAKALPGVVAVVTGADAPWTHGEAVKDKPFLAQGKVRYIGEPVAAV
                     AAEDEDIAQAAVKLIKVEYEDLPVYIDPEEACKPGCMEIHEDYDSYRKANFMVRGAAP
                     NIAEHFKLRTGDVAKGFAESDLVLDEKYFVPIIQHAAMEPHSAHAQWDKNSGRLTIWV
                     ANDAPFRALHEISEALGMEKEKIRFINPAQGGGFGSKGGLKCEPIAVALAFHTNGRPV
                     RVKFNREETFISTLTRHAATVYSKTGVKKDGTLVAREMTIYWDAGAYAEKSPTVCIRG
                     SLPAPGPYRIPHVKVDGYAVYTNKPVAGSYRGYGIPQGAWAGEQQMDELARKLGIDPI
                     DIRMKNIFVEGDMSYWGEQLHAVGLKETLIKATEAIEYGKRSSINKPGVKIGKGFACI
                     QKPTRSPTTSNAGVTVNAQGEVTVFAGTVEIGQGCNTILSQVAAEELRVPMNKVRIHQ
                     LDTDVIPYDASTTSSRSTYHMGNAVRGASIHAREQIMEAAAPMLEVGTRDLSVADGKV
                     FAKDQPQQALPIGEVVKRKLGINGIVRGDASYTYEIGKDLDLETGHSDHASAFYMYAT
                     QAAEVAVDEESGRIRVLRMSAAHDVGKAINPLNCAAQIEGGVVMGIGSAIHEELVIDN
                     TGKVRNPSFLDYHLVTSLDCPEMIPIIVECAEPEGPYGAKGLGEPGLAPTPAAIGNAV
                     ADATGVRIYDLPLKPENVFWALQKNKKSAA"
BASE COUNT         1680 a         1824 c         1811 g         1362 t
ORIGIN      
        1 actgcctgta gttttgtctg tgccacaatg cctcctaaat tattttggcg ttctgctgca
       61 aattactggt tttaagttca accaaaagcg agaatcggcg ctttggtcgc aagactcatt
      121 atcattttcc accgtttatt caagcaaaaa ctttcctacg ttgacaagcc ttagcggatc
      181 ggatagtttc cgctgcgttg cataaaatag tttatgcaaa gtagcccgat gaattttcca
      241 atcaagaaat caaaaacgaa aacgcacgcc gtcgaaaacc tctcagcccg cgtttacaat
      301 caaattaaac aattgatcct gtgcaacgaa ataatgccag gacaaaaact gcatcatcaa
      361 caactcagtg aacgtctcgg cgtcagtcgc actccggttc gtgaagcatt gacccgactc
      421 gtgcaggaag gctacgtttc ctttctgccg aaccgcggtt ttacctgcaa agaaattaga
      481 ttacaagagg cggaagagct ttacgaatta cgcgaatccc tcgaggcctt tgcggtggaa
      541 aaagcggttg aaaattcgac cgaccagggt ctcgcggagc tgcgccagag aatggaatcc
      601 tacggccatg atgtctacaa ccgatttacg cgagaccgct tggtctacga ccaggatgtc
      661 catcttcata tcgcgcaaat cgccggcaac gagactttaa aaaagacctt gagccacgtc
      721 tttgagcgca tcgtgctcaa acgccgcacc gacggtatat acgatgcggc acggggactt
      781 accgcccatc aggaacatgt tcaacttctc gatgcgatga cgcgccgcga cgcgaaagca
      841 gccgttagca tcgtgcgcca ccacattcag gctggcaagc aaaacgtcat tgccgaccta
      901 cgacagcgtc aagcgatccg cgaattgcgg cccgctgact taatatagga cgaaataaac
      961 aatcagagaa agggagatta tatggcagca gatctagtga ttaagggcgg ttgggtggtc
     1021 acacccgatg caacattcaa aggcggcgta gcgattagca atgaggtcat cgtcgccata
     1081 ggcgcggacg attcactacc cgacggcaaa gaggtcatcg acgtcaaggg gaaacacatt
     1141 ctgccgggtc tgttcgacgg acacgttcac ttccgtgaac ccggcatgac ccataaggag
     1201 gatttcaact cgggttccac ggcagccgta tgcggtggca tcacgacggt ggtggacatg
     1261 cccaacactg tgcctccgac ggcagacgcc gagcaagtta aagttaaaaa ggagcttggc
     1321 gaggccaaat cgttggtcga ctttggtatt accggagtcg tcgtgcaaac caacaccaag
     1381 gacatcctac ccatggcggc ggccggcgcg atcggctaca agattttctt cggcgaaacg
     1441 atcggcaacc tgcccttccc cgatgacggc atgtgcgtcg aagccttcga ctacatcgct
     1501 cagtcgaagt tgcctttggg aatccacgcg gaaaaccgcc agatcatggc gtactgcacc
     1561 aacaagctga aaactgaagg caagaatgat ccgcgctatt gggaagcttc ccggccggat
     1621 atctgcgaag cggagtcggt gcatcatgcg attttcttcg cggaaacctt cggcaccaaa
     1681 ctccatgtct tccacatgag ttcgaagcag gccgcttata tggtccgcga tgccaaagcg
     1741 cgcggcatga gaatcaccgc ggaaaccggc ccgcattatt tgctgcgcga acccaatgac
     1801 atggacaaag tcggttcgtt gctgaaaatg aaccctcccg tccgcaccaa agaccacgcc
     1861 gaagtccttt ggcaggggtt gcgcgagggt ttcgtggata tgttagccac cgaccattcg
     1921 ccgcacacgt tggacgagaa aggctcggac atctacggca agctcaccaa gccggcgatt
     1981 tgggactgca tttcgggttt ctgcggcgtt gagactggcg tgccgctgat cttgactgaa
     2041 gtgaacaagg gccgcttgac cttgaatcaa tacgtcaaag tcgcctccga aaacccggcc
     2101 aaagtttggc aggtctatcc gaagaaaggc gcgatccggc tcggctccga cggcgatatc
     2161 accgtcgtcg acatggataa agaaggtacc atcgacgtca ataagctgca cagcaagaac
     2221 aaaccgtcgc cgtggcatgg ttggaaagtc aaaggcatgc cggtgctcac cgttgtgcgt
     2281 ggtcacgtcc agatgaaaga cggcgaaccc tgcggcaaag cgatcggtaa agcgctcaag
     2341 ccgatcatct aagggttggc aaatggagtg atggagtatt gggtacggac tcaccgcgcc
     2401 caaacaccca gtatttcaca ctccatacgc atccgcaccc gcctatcgtc gcaaggaagc
     2461 catgtgcctg agattactcg gacggattaa ggaatcgttt catgaaacgc acggaagcat
     2521 ttgagatgtt tcaaccggcc actctcgaag aagcgagccg actgctaaaa gaaaaaggca
     2581 ccggcggtcg ttttctcgcc ggcggcacgg acttggtgat cgccatgaaa gagaaagggc
     2641 tgctacctaa atacatagtc gatctgaagc gattgcctgg tctcggcggt attcgcgaga
     2701 acagcgacgg tagcattagt ttaggcgcgc tgaccacgtt gcacgagatc gaaacctcgg
     2761 cgctgatcaa gagcaaatat ccatttttgg cgcaaagcgc cgccgaagtg ggctccatac
     2821 agatccgcaa ccgtgccact attggcggca acatggccaa cgcttcgccg tcggccgata
     2881 cttcaccggc attgattgcg ctcggtgcca gcgtgaccat cgccagtggc agcggtcaga
     2941 ggcaaattgc cgcagaagat tttttcagag gtccgggtca gaatgctatg aactcggacg
     3001 agattttgac tgaagtcaac atccccaaaa ccggcgcgaa cctggtcggc gaatacatca
     3061 aattctcacc gcgcgaaatg atggatctgg cttacgttgg ggttgccgta gtttataatt
     3121 tagctaggag cgatagaaga tgcactggag ttcggatcgt tctcggcgca gttgccccga
     3181 cgccgatccg cgccaaacgc gccgaagcgg ctttggaaag ccaggtactc agtgaagcag
     3241 tggctgaaaa ggccggccag atcgccgcgg aagaagccaa accgataagc gacgttcgct
     3301 cgagtgcgga ttatcgccgc gcgatggtcg gcactatgac caaacgagcc ctgctcaatg
     3361 ccgctgtcgg cgccgccaaa tcctgggtcg aacggcgcga tcgaaggtac taaggccacc
     3421 gctgatctga gtattggagt tgggttgagg taggacttgc ctcaacattt cagtactcca
     3481 atactccatc actcgaagtt caagtgaggt tatcatgaac aaacaaattg aatttactct
     3541 caacggtaaa tcagtcagcc gtgaagtccc gacgcaccgc ctgcttctcg atttgctgcg
     3601 cgatgagatc ggcatgaccg gcaccaaaga aggttgcggc accggcgatt gtggcgcctg
     3661 caccgtcctt cttgatggca agcctgtcaa ttcctgtctg gtgctttccg gcgaactcga
     3721 cggcgccgac attgtgacca ttgaggggtt gcgaatcggt ccggaactgc atccggtgca
     3781 acaagccttc gtccaggacg gcggcgccca gtgcggctac tgcacacctg ggatgttgat
     3841 gatgtcaaaa gcattgcttg atgaaaaccc caatccaacg gacgaggaga ttcgctacgc
     3901 actctccggg aatctctgcc gctgcaccgg ttacgccaaa atcgttgagg cggtaaaaga
     3961 cgcggcgaca ataattcgca atggttagaa aatgttaaaa ggaccaagtc gttcaatcgc
     4021 tgctctgctc aaaccgttga atccttaata gcgcggtttg cgcaacgtac ctgcgaatct
     4081 taattggcgg ctgaacagtt taaacggtct ggacaacttt taggagtaat tgagatggcg
     4141 gaacttactg taatcggcaa accggtcacg cgcgtcgatg ccaacgaaaa agtcacagga
     4201 cagatcattt acggctatga tttggccctg ccgaacatgc tctatggcaa gacgctgttc
     4261 agcccgaagg cccacgcgaa gattaagaaa atcgatacca ccaaggccaa agcgctgccc
     4321 ggtgtcgtcg ctgtggtcac cggtgcggat gctccttgga cgcatgggga agccgtcaaa
     4381 gacaagcctt tcctcgccca gggcaaagtg cgttatattg gcgaacctgt ggcggcagtc
     4441 gccgctgagg acgaagacat cgcccaagcc gcggtcaagc tcatcaaagt cgagtatgag
     4501 gacttacctg tttacatcga tcccgaggag gcctgtaaac ccggctgcat ggagatccac
     4561 gaagattacg acagctatcg taaggccaac tttatggtcc gcggcgcagc gcctaacatc
     4621 gccgaacatt tcaaactgcg caccggcgac gtcgccaaag gatttgctga atcggattta
     4681 gtgttggacg aaaagtattt cgtcccaatc attcaacatg ccgcaatgga gccgcattcg
     4741 gcgcatgccc agtgggataa aaactccggg cgcctgacga tctgggttgc caacgatgcg
     4801 cctttccgcg cactgcacga gatctccgaa gctctcggca tggaaaaaga aaagattcga
     4861 ttcatcaacc cagcccaagg cggcggcttc ggctccaaag gcggtttgaa atgcgaaccg
     4921 attgcggttg ccttggcgtt tcataccaac gggcggccgg tgcgcgtgaa gttcaaccga
     4981 gaggaaacct ttatttctac cctgacgcgc cacgcggcaa cggtctattc caagaccggt
     5041 gtgaagaaag acggcacgct tgtcgcgaga gagatgacca tctactggga tgccggcgcc
     5101 tacgcggaga aaagcccgac tgtttgcatt cgcggcagcc tgcccgcgcc cggtccatat
     5161 agaattcccc acgtcaaagt tgacggctat gcggtttata ccaacaagcc ggttgccggt
     5221 tcttatcgcg gctacggcat tcctcagggc gcctgggccg gcgaacagca aatggatgaa
     5281 ctggcgcgca aattgggaat tgacccaatc gacattcgta tgaagaatat cttcgttgaa
     5341 ggcgacatgt cctattgggg cgaacagctt cacgctgtcg gcttgaagga aactttgatc
     5401 aaagcaaccg aggcaatcga atacggcaag agatcatcga tcaacaaacc gggcgtgaaa
     5461 atcggcaaag gcttcgcctg catccaaaag cccacccgct cgccgacgac ctctaacgcc
     5521 ggcgttaccg tgaacgcgca gggcgaagta acagtgttcg ccggaaccgt ggaaatcggc
     5581 caaggttgta acacgatcct gtcacaagtt gccgccgaag agttgcgagt tccaatgaac
     5641 aaagtgcgca ttcaccaact cgacactgac gtgattccct acgatgcgtc gacgacctcg
     5701 agccggagca cttatcacat gggcaacgcc gtgcgcggcg catcgattca cgcgcgtgag
     5761 caaatcatgg aagcggcggc gccaatgctc gaagtcggca cacgagatct ttccgttgcc
     5821 gacggcaaag tcttcgctaa agatcagccc caacaagctc tgccgatcgg cgaggtggtc
     5881 aaacgcaagc ttggcatcaa tgggatcgtc cgcggcgacg catcctatac ttacgagata
     5941 ggcaaggatt tggacctgga aaccggccat agcgatcacg cctccgcctt ttatatgtat
     6001 gccacgcaag ccgctgaagt cgcggtggac gaagagagcg ggcgcattcg ggtgttgaga
     6061 atgtcggcag cccacgacgt cggcaaggcc atcaatccgc ttaattgcgc cgcgcaaatc
     6121 gaaggcggtg tagtcatggg tatcggctca gccatccatg aagagttggt aatcgacaac
     6181 accggcaaag tgcgcaatcc ctcgtttctt gactaccatt tagtcacttc gctcgattgt
     6241 ccggaaatga tcccaatcat agtggaatgc gccgagcccg aaggacccta cggtgccaaa
     6301 ggcttgggcg agcccggtct agcgccgacg ccggccgcca taggcaatgc cgtcgccgat
     6361 gccacaggcg ttcgtattta cgatttgcca ctcaagccgg aaaatgtttt ttgggcgctg
     6421 cagaagaata aaaaaagtgc tgcctgagca ttccgcctcg aatttttgaa gcccttcgct
     6481 tacaatttgc cgaccggttc taagccggtg aattgccttg tgatggagaa accctaaatg
     6541 cgaaccgctt ttcgccaatt gttcctgcag ctaagtatgg ctgtctcgat cctcccaacg
     6601 cttgcgagca tgggagcaga gacgggacag cgcacggttc aaacagtatc gggcatgccc
     6661 gcggtcattg accacaa
//
LOCUS       DATLCH010000304         6056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7161330, whole genome shotgun sequence.
ACCESSION   DATLCH010000304 DATLCH010000000
VERSION     DATLCH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7161330"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VMZ02_08945"
     CDS             complement(<1..1095)
                     /locus_tag="VMZ02_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72101.1"
                     /translation="MDWDIGVVRYLPQDEMRIPRGPDGKKVGVFLLHGGVSDFKSVER
                     IAKILPEKFGIPVATMTFPGRFYFHDANRDWPGDVENSDGSARTPLWSRETKITPDQY
                     SIVRDTSKREDYGTLVSLAAKEGTEFYYRMAAWPVAFEQALKEAARRQFPEAEYSIYI
                     HGHSTGGPFAMMAAQRVANIAGLLGWGSSPFGWMYPLVSGDDWNFPFNQLRLRTWRDT
                     ARYMYEGMKHEEIGLPMLIELTFERWESAKKRPNFKAEDFIHKNSTKALEAAACASAA
                     RLKLSHQETDVLVQRYLGYTKELSGQGVKPVPNFLSIHGVNDDTVTLKRCERSLPLFA
                     KLNPPPRVCPVSLGAGIHSWGWANDKLPQGI"
     gene            complement(1263..3191)
                     /locus_tag="VMZ02_08950"
     CDS             complement(1263..3191)
                     /locus_tag="VMZ02_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HUR72102.1"
                     /translation="MINQSLALSVIVYGILTLHCTRPSLIHAQPVSKAIVMAWDGTVP
                     AFVKQLVQDGKLPNLAKLIAGGAFADTVMPGYPSKTAPGFASLMTGAPPRVTGISGNR
                     VPRAPRDQFTILESLPGFAGAPLRAEPIWATARRAGKTSVVSHIPSFAEERSESLVRF
                     AGYDLVVGRDGIVTRRAIKSESSPPWKNPPASDAPPIEFEFTAGESLLFGLLIDDPAD
                     VQIGYDTAIISTDRDGREIKTKLKPAPSGPGGELFWSRPIAVRASGTSNAKVYFRLFD
                     IKADGSDFFLYFTRPTPDLELKQDTEASPTVRTFIGNGASILYQNGALGRTVAHGGNG
                     GAEARYLETILFAQHQLMETNRWAIENLPWDLFLAYTPFPDEAEHAWRGHLDTTLPNF
                     RPDIAEKLRPFLEQVYRSSDEHLGMLLSKRPENTAFVVISDHGMQGIYKKVALNPFLQ
                     DKQFLVLDSQNRVDLTRSKIMYPTVNSGYLVINSIDRKGGIVRDDERDELVRNLREAL
                     FALRDGERQVVISIIDAASEGDEKGIGGEVGGDLYIELAPGYDFDPRLSGGQWITDAE
                     PYGTHGASPDQASMRTLMVFNGPGIRPGQGLTNARLIDFAPTLAWILGLPRPKDATGS
                     VLYEAFSETRVDIANPLP"
     gene            complement(3243..4226)
                     /locus_tag="VMZ02_08955"
     CDS             complement(3243..4226)
                     /locus_tag="VMZ02_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72103.1"
                     /translation="MVPSKRIAFSLWLAITMLVTPLQAQTAPDKITLGTITLSLNNLP
                     IYVAQEKGFFTKENIFLEAVVLNASTLAIPVLIAGSTQVSASSAMTTIRAVEKGASLK
                     IVGGLTNAPVYDLYANPKYKSFKDLKGASIGVSGLITSDTVIMKEMLKANGLEYPRDY
                     ALRAMGSMPDRFLAMQTGNIAAAILSPPYTFGADEAGLINLGSTAKYTPNFVQTVFNV
                     RTDFAQDKKPLLIRFLRAILRGDQWIHNQKEDTVRIIMKRLKYSEKHSEASWRYYINT
                     NAIPKDGEINPKGMDKVMQLLVEDGTLVQPLPKTDKYVDMSYLAEARRTLQ"
     gene            complement(4363..5643)
                     /locus_tag="VMZ02_08960"
     CDS             complement(4363..5643)
                     /locus_tag="VMZ02_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HUR72104.1"
                     /translation="MRRAFGLDFGTTNSALALAHSESAVELARFGDGSTFRSILYFDE
                     EETSRSGKLRVVAGPEAIHCYLNARTPGRLIQSTKSYLASRLFSQTQIFNQSFSLEEL
                     IGILLRHLRKAAELQFGDLATELVVGRPVHFSGTKNHADDNFAVTRLRKALANAGFEE
                     VHFLPEPVAAAYKYRQRLDHDELVLIADFGGGTSDFSLVHLQTTQRSITELNDQVIGN
                     DGVGIAGDTFDSKLVRHLVAPLLGLGSEYRSQFGKLLPAPNWLYEHLERWHYLSFLKT
                     RKNMELLKQIRFQSLAPEKLDALIDLVEHDLGYRLYQAIESVKCNLSEQAHSQFHFSE
                     AAIAIGQSVARSDFEQWIAHEIRAITMCVDKLLASCAVAAGDVDAIFMTGGSSFVPAI
                     RTIFETRFPRAPIRAGQEFTSVAEGLALHALEYL"
     gene            5851..6027
                     /locus_tag="VMZ02_08965"
     CDS             5851..6027
                     /locus_tag="VMZ02_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72105.1"
                     /translation="MAQVSNCELDGIMFAQKSTKTSLAGQWVFTFQVKPAGDYTDPSS
                     TLGRFVEHAIAIKS"
BASE COUNT         1316 a         1600 c         1752 g         1388 t
ORIGIN      
        1 gatgccctga ggcagtttgt cattggccca gccccatgaa tggataccgg cgccgagcga
       61 gaccgggcag actcttggcg gcggattgag tttagcgaat aacggtagcg agcgttcaca
      121 gcgtttcagc gtgacggtat cgtcgttgac gccgtggatc gacagaaagt tgggcaccgg
      181 tttgacgccc tggcccgaca attcttttgt atagccgagg tagcgctgga cgaggacgtc
      241 ggtttcttga tgggagagct tgagcctcgc ggcactggcg caggcagcgg cttctagagc
      301 cttggtcgaa ttcttgtgaa tgaaatcctc ggctttgaag ttgggccgct tcttggcgct
      361 ttcccaacgc tcgaaagtca gctcgatcaa catcggcagg ccgatttctt catgtttcat
      421 gccttcgtac atataacgcg cggtgtcgcg ccatgtgcgc aggcgcaatt gattgaaggg
      481 gaaattccag tcgtcgccgc tgacgagggg atacatccaa ccgaaaggcg agctgcccca
      541 accgagcaac ccggcgatgt tggcgacacg ctgcgctgcc atcatcgcga atgggccgcc
      601 ggtggaatgg ccgtggatgt agatcgagta ttcagcctcg ggaaactgcc ggcgcgccgc
      661 ttccttgagc gcttgttcga aggcgaccgg ccaggcagcc attcggtagt aaaattccgt
      721 cccttccttg gccgctaacg aaaccagcgt gccgtagtct tcccgtttcg aggtgtcccg
      781 cacaatactg tactggtccg gagttatttt agtttccctg gaccagagcg gcgttcgtgc
      841 cgagccgtcg gagttttcga cgtcacccgg ccagtcgcga ttggcgtcgt gaaaatagaa
      901 acgacccgga aacgtcatcg tcgctaccgg aatgccgaat ttctcgggca agatcttggc
      961 tattcgttcc actgacttga aatccgagac accaccgtgc aggagaaaaa cgccgacttt
     1021 ctttccgtcc ggtccgcgtg ggatgcgcat ttcgtcctgt ggaagatagc gcacaacccc
     1081 aatgtcccag tccatggcca gctcgtggat tcgaaagatg ttctcgctga ttgcgatcgg
     1141 ggcgccgctt ttacaactaa cttcgtcgtt aagtttgagg acctcgtcta aggagattga
     1201 cgccgggggt tcccaagctg aattaattgt cacagtgact cctacgatat cgtgtcaaga
     1261 aactatggaa gcgggttagc gatgtcaacg cgcgtttcgc taaacgcttc gtacagcacg
     1321 ctgccggtcg cgtctttggg ccgcggcaaa cctaatatcc aggcgagggt tggggcaaaa
     1381 tcgattagcc gagcgttggt aagtccctgc ccagggcgaa taccgggacc gttgaatacc
     1441 atgagcgtgc gcatggaggc ctgatcggga ctagcgccgt gggtaccgta gggctcggca
     1501 tccgtgatcc attggccgcc gctcaggcgc ggatcgaaat cgtaacccgg ggcaagttcg
     1561 atgtagaggt cgccgccaac ctcgccgccg attcccttct cgtctccttc ggacgcggca
     1621 tcgattatcg atatgaccac ttggcgctcg ccgtcgcgca gcgcgaaaag cgcctcgcgc
     1681 aaattgcgaa ccaactcgtc acgctcatca tctctaacga taccgccttt gcgatctatc
     1741 gagttgatca ccaaatagcc gctattgacg gtggggtaca tgattttact tcttgtaaga
     1801 tcgacgcggt tctggctatc gagcacgaga aattgtttgt cttggagaaa ggggttgagt
     1861 gcgacctttt tataaatgcc ttgcatgcca tggtcggaga tcacgacgaa cgcagtattt
     1921 tctggccgtt tcgacagaag catgccgaga tgctcgtcgc ttgagcgata cacttgctct
     1981 agaaacggcc ggagcttctc ggcgatgtcc ggccgaaaat tgggcagcgt tgtgtccagg
     2041 tggccgcgcc atgcatgctc ggcttcgtcg ggaaacggcg tgtaggcgag aaacagatcc
     2101 cagggcagat tctcgatggc ccagcgattg gtttccatca actggtgctg ggcgaaaaga
     2161 atggtctcga gatagcgcgc ttccgcgccg ccattaccac cgtgcgcgac ggtgcggccg
     2221 agcgcaccgt tttgatagag aatactggcg ccgttgccga tgaaggtgcg cacggtaggg
     2281 ctggcctcgg tgtcttgctt caactcaagg tcgggagtcg gtcgagtgaa atagagaaaa
     2341 aaatcgctgc cgtcggcttt aatatcaaaa agacgaaaat acactttggc gttgcttgta
     2401 ccgctggcgc gaaccgcgat cgggcgcgac cagaaaagtt ccccgccggg acccgaaggt
     2461 gccggtttca gttttgtctt aatttcccgg ccgtcgcgat ccgtgctgat aatcgccgtg
     2521 tcataaccga tttgcacgtc ggcagggtcg tcgatcagta gtccaaagag caaagattca
     2581 cctgcggtga attcaaactc aatcggtggc gcgtcgcttg cgggcggatt tttccaaggt
     2641 ggactgcttt cactcttgat cgcgcgccgc gtgacgatac cgtcgcgccc aacgactaga
     2701 tcgtagccgg cgaagcgcac gagggattcc gatctttcct cggcaaacga tgggatatgt
     2761 gacactaccg acgttttgcc tgcccgtcgg gcggtggccc agatgggctc ggcacgcaaa
     2821 ggcgcgccgg cgaaccccgg aaggctttcc aagatggtaa actgatcgcg cggtgcgcgc
     2881 ggcacgcgat tgccgctgat accggttaca cgcggcggtg cgccggtcat cagcgaggcg
     2941 aatccgggtg cggtcttcga tggatatccc ggcatcacag tgtcggcaaa ggcgccgccg
     3001 gcgatcagct tggctaaatt gggaagtttg ccgtcttgca cgagttgttt gacaaacgct
     3061 ggtacggtgc cgtcccaggc catgacgatc gctttggata ccggctgggc atgaatcagc
     3121 gagggacggg tgcaatgaag agtgagaatc ccgtaaacaa ttacgcttag cgcgagtgac
     3181 tgattaatca ttgaaactgg cggtcattga ctggcgatgg cgacgtggaa ttctacgaca
     3241 gctcactgca aggttcgccg cgcttcggca agatagctca tatcgacata tttatcggtc
     3301 ttcggcagcg gttgaaccag ggtgccgtct tcaacgagca attgcatgac cttgtccatg
     3361 cccttgggat tgatttcgcc gtctttgggg attgcgttgg tattgatgta gtagcgccaa
     3421 ctcgcttccg aatgcttctc gctatactta agccgtttca tgatgatgcg cacggtgtct
     3481 tccttctggt tgtggatcca ttgatcgccg cgcaggatcg cccgcaagaa gcggatcagc
     3541 agtggtttct tgtcctgggc gaagtcggtg cgcacattga aaaccgtctg gacaaagttt
     3601 ggcgtgtact tggcggtgga accgaggttg atcaagccgg cttcgtcggc gccgaaggtg
     3661 tagggcgggc ttaaaatcgc cgcggcgatg ttgccggtct gcatcgccag aaaacggtcc
     3721 ggcatgctgc ccatcgcgcg cagcgcgtaa tcgcgcgggt attccaaacc gttggctttg
     3781 agcatttctt tcatgatcac ggtgtcgctg gtgatcagcc cggagacgcc gatgctggcg
     3841 cctttgagat ccttgaagct tttgtatttg ggattggcat agagatcgta caccggggca
     3901 ttggtgagcc cgccgacaat cttcaaggac gcgcctttct caacagcgcg gatagtggtc
     3961 atcgccgacg atgcggatac ctgggtcgag ccggcgatca gcactggaat cgccagcgtg
     4021 cttgcgttga gcaccacggc ctcgagaaag atgttttctt tcgtaaaaaa acctttttcc
     4081 tgggccacgt agatcggcaa attattaagc gacagtgtga tcgttccgag agtaatctta
     4141 tccggtgcgg tttgggcttg caggggtgtg accaacatcg tgatcgcaag ccacagcgag
     4201 aaagcaattc tcttcgacgg aaccatcgta cctccaaatt caatcttcat cggcatcgac
     4261 agtagccgat tgcgaaacaa agtgtcaacg cggagatgaa cttagattag cagtgtcgta
     4321 atgccgaatt ttattgaatg catgttgggc aataaatctt gcttataaat actccaaagc
     4381 gtgcaatgcc aagccctcgg ccaccgaggt gaattcttgc ccggcgcgaa tcggcgcgcg
     4441 cggaaatctc gtttcgaaaa tggtgcggat cgccggcaca aacgatgaac ctccggtcat
     4501 gaagatcgcg tccacgtcgc cggcagcgac ggcgcagctg gcgagcagtt tgtcgacgca
     4561 catcgtaatt gctcggattt cgtgagcaat ccactgttcg aaatcgctgc gcgcaacgga
     4621 ttgcccaatg gcgatggcgg cttcgctgaa atgaaactgg ctatgcgcct gttctgataa
     4681 gttgcattta accgactcga tcgcttggta tagacggtaa ccgagatcat gctcgacgag
     4741 gtctattagc gcatcgagct tttccggagc cagggattgg aaccgaatct gcttgagcag
     4801 ttccatgttt ttccgcgttt tcagaaacga tagatagtgc cagcgctcca aatgttcgta
     4861 cagccaattg ggcgccggca ataatttgcc aaattgcgat ctgtactctg agcccaagcc
     4921 caacaacggt gcgaccaaat ggcgaacgag tttgctgtcg aatgtatccc ctgcaatacc
     4981 gacgccgtca ttaccaatga cttgatcgtt caattcggtt atcgaacgct gtgtggtttg
     5041 caagtgaacc aaggaaaaat cactggtgcc gccgccgaag tcggcgatca gcacgagctc
     5101 gtcatggtcc agacgctggc ggtatttata cgcggcggcg accggctcgg gcaaaaagtg
     5161 aacctcttcg aatccggcgt tagcaagagc tttgcgcaat cgcgtgaccg caaaattatc
     5221 gtccgcgtga tttttcgtac cgctgaagtg caccggccgt ccgaccacga gttccgtggc
     5281 caagtcaccg aactgtaatt ctgccgcttt gcgcaagtgg cgcagcagga taccgattag
     5341 ttcctcaagc gagaaacttt gattgaatat ctgggtttga ctgaatagcc ggctagcaag
     5401 ataggatttg gtagactgaa ttagccggcc cggcgtgcgt gcattcagat agcaatgaat
     5461 cgcctccgga cccgcgacta cgcgcaactt gccgcttctc gatgtctcct cttcatcaaa
     5521 atagaggatc gagcgaaaag tcgaaccatc gccgaaacgc gcgagttcga cggcgctttc
     5581 tgagtgggcg agcgccagag cgctgtttgt cgttccgaag tcgagtccga aggctctacg
     5641 caaggcaaac gccgaccttt cgaataataa aatccggaat tatcgatact agcatcgtcg
     5701 gcaaaattac caagcataat aggttggctt gctcatgcca cggggtgcgc acgtaatctg
     5761 acagttgatg aagttcgctg acgcattgtg ccggtggcat ggacctaatt gaggcggggt
     5821 cattacctag tactccggag aattctcgaa atggcacagg tgtccaactg tgaactggac
     5881 ggcatcatgt ttgctcagaa aagcacgaag acttccctgg ctggtcaatg ggtgtttact
     5941 ttccaggtca aaccggccgg agactatacc gaccccagtt caaccttggg cagatttgta
     6001 gaacatgcga ttgcgattaa atcctagctt gcgcgcacgt ctgttcgaaa tagtag
//
LOCUS       DATLCH010000305        13601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8657287, whole genome shotgun sequence.
ACCESSION   DATLCH010000305 DATLCH010000000
VERSION     DATLCH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8657287"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VMZ02_08970"
     CDS             complement(<1..324)
                     /locus_tag="VMZ02_08970"
                     /inference="COORDINATES: protein motif:HMM:NF036998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72106.1"
                     /translation="MPGKQNLTLLTDLYQLTMAQAYFREQRLDAATFSLFIRSYPLNR
                     GYFVAAGLRDVLDYLETFSFNNSALDYLAAQGFFTDDFLHYLSDLKFTGEVWAIPEGR
                     LFFKDE"
     gene            468..1190
                     /locus_tag="VMZ02_08975"
     CDS             468..1190
                     /locus_tag="VMZ02_08975"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HUR72107.1"
                     /translation="MFTDKVDLKDFDKQDPEYQDLLRRVLSIQADCEIGGPNLYVKDI
                     LPNAPTKLDQLIVARTAAEEMDHFRKIAKLAGDIGVDVSFVLSRPNKDRYVEAFRGEI
                     KTWEDYAVFGFLIDRVGRFQLEEFIGCTYLPLARVVEDPEMIREEEGHIDFGVNASAE
                     WAAKGGETKERIQKAVNYWYVKGLDMFGNSKSYRSERYMHWGLKRRTNAEARALYKEE
                     IDGLLRKMDLDIPDPNQGRLYT"
     gene            1210..1401
                     /locus_tag="VMZ02_08980"
     CDS             1210..1401
                     /locus_tag="VMZ02_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72108.1"
                     /translation="MEAAPNTDALDQQFKEFIQALKKAVGTGIPHLKDDPKIVDQAIV
                     DCKEILDVVMEGNVKEWIS"
     gene            1516..2295
                     /locus_tag="VMZ02_08985"
     CDS             1516..2295
                     /locus_tag="VMZ02_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HUR72109.1"
                     /translation="MNVIVCGKVIPASTVTIEIDANTKRLVRKGVPHELDPAAASAVE
                     EGLRLTEKLGGTVVLVTMGTSDATIGIRNALAMGVTSAVHILDDVVAGSDTLGTAKLL
                     AAAIKKQDYDLVICATESGDSYSGIVHGQIAQLLGLPPLTFAKEITVDGGNITIKRQS
                     ESGFEIVESTLPALVACSSGINEPRYPQLKGIMAAKKKEIKVYSAADLGLSPEQVGEK
                     GAREKVLTIGRPPKRQAGKAVTDEGEGGKQIADFLAEIKVI"
     gene            2295..3263
                     /locus_tag="VMZ02_08990"
     CDS             2295..3263
                     /locus_tag="VMZ02_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HUR72110.1"
                     /translation="MAETIWVYAEISEDKITTTSLEMLAKAAEVGAAEAILLGPAPDD
                     AVQTLANHGASKIYRSTDAVYKDYLTLPAAETVAALIQKHNPSVMLFASSYAGRDLVA
                     NLSARLDCGAITDVGDFELKDGSVVATIPALGASYQNTSTLLTSGTKLLVVRPKSFEP
                     KENPQALAVEEVPAAASADLRKVHMKERVVVKREGPSLEGAKIVISGGRGLKGEEQFS
                     ILKDLADVLGGAVGASRAAVDAGWVPYAMQIGQTGKTVKPDIYFAVGISGAVQHLSGM
                     KTAKYIITINKDPDAPIFQYSDFGVVGDLFKIVPQLIEELKKRKGA"
     gene            complement(3590..4168)
                     /gene="gfa"
                     /locus_tag="VMZ02_08995"
     CDS             complement(3590..4168)
                     /gene="gfa"
                     /locus_tag="VMZ02_08995"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HUR72111.1"
                     /translation="MATPLSIHPAVDQGIKPGAANFAGGSLLCRCADKKVAVAIKGQC
                     AHNHVCGCTKCWKPAGALFSQVAVVSRDNLSVTANGDKLKVVDANAAIKRYACSGCGV
                     HMYGRIDNTKHPFYGFDFIHTELSSEPGWAPPEFAAFVSSIIESGADPANMGAVRARL
                     KELKLEPYDCLSPVLMDAIATHTAKATGVLKG"
     gene            4341..5387
                     /locus_tag="VMZ02_09000"
     CDS             4341..5387
                     /locus_tag="VMZ02_09000"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72112.1"
                     /translation="MKRSFPVIDADGHILEKDHELREFLPPEFRAVPGKREYPLFSWD
                     GWARGALSPTKREHPSVELWLRFLDATEIALTVLYPNEGLNIGLQRDRDWAVGLARAY
                     NDWLHHDFLSVSPRFQAVALLAPQEPQEAAKELARAVHERGMIAGMLPGVMSPMKGLG
                     LPEFDPIYAMAEKLDVPLAVHSGPAVELGLEHMQSFAGVYPLSHPFAQMQQLTSMMIY
                     GVFERFPKLRVAFLESGCGWVPYMADRLDESWEHRRNRWPHPAKRSPSSIMSGGNLYY
                     ACEREERTLATVAGIIGAGQLLWASDFPHERPWDEFSGDIDTLVNRKDLPESLPRKIL
                     FDNPRRFYNLKVDG"
     gene            5499..6413
                     /locus_tag="VMZ02_09005"
     CDS             5499..6413
                     /locus_tag="VMZ02_09005"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR72113.1"
                     /translation="MRVLITGAGLIGTHTAKELIDRRDDVTFFDFAPRPDYIRHVTGN
                     DVPVLRGDIRDLAALIDACQTVRPDCVIHLAASVGEANINNVYAGFQINLVAAINVAE
                     AVRLAGVRKLVHASTQAVYSGEDPKTLLREDSPIDCRERVYNASKIGCEHVLRTYAAK
                     HNIELALLRFAGVYGYYSVAGGPGVAVQQAVWDAMAGRAAVLNVYEAVDFIYVKDLAN
                     GIALAVHTASLPHNVYNLGSGRLTTVADVESALAAIFPGINMKRGKLTPARPRMDIIR
                     AETELGYKPEYKLETGLRDYVTELKNGS"
     gene            6503..7474
                     /locus_tag="VMZ02_09010"
     CDS             6503..7474
                     /locus_tag="VMZ02_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HUR72114.1"
                     /translation="MKIAVIDDYQNAFKTLKCFPKLAGHEVVVFTDTANDVASLAEKL
                     KDADAVVLTQQRSYIPRELIEKLPKLKLIGQTGRAATHVDLQACTEKGIIVSAGGSGN
                     PNATAELTWGLILSSLRSLPREVTRLREGQWQSTLGTGVSGKTIGVYAYGKIGSIVAA
                     VGKAFGARVVCWGREGSTGRAKAAGFEVANSREEFFAEADIISLHLPLNQDTRGIVNA
                     GDLARMKPSALLVNASRSGLIAKGALVEALNAGRPGFAAVDVYDKEPVGADEPLIKMA
                     NVTCTPHLGYVTRESYEEYFAVVVDDIVSFAAGKPSHVLNPEALGKK"
     gene            7747..9477
                     /locus_tag="VMZ02_09015"
     CDS             7747..9477
                     /locus_tag="VMZ02_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HUR72115.1"
                     /translation="MPAATEPCAAIDGIQGNRWLHVFLLIALCGVLYFPYLGSTPVFD
                     KGEPREGLAVQDIVQRGEWLFPLKRAAAIPSKPPMFHWSAALVYQFTGVLDEATLRLP
                     SAVYATLGVISLYLLGAKLFGAQVALLGGAILATTMIYQTQAVNARVDMTLCFFVTLS
                     LILFYSIYRGLLTHPLWPYAMYLLVGLATLSKGPLGLVLPGLVIAVFLALKRNWQQFL
                     RFAFHPGVLIAVILVLGWYGLAITRGGEGFVDRQLLSENLGRFFGGSGHSHPLYYYLG
                     YLFSLAIPWGLFYPLMFWDWAEHRGSAEGDDVRFLQTWFAAMVIFFSLSSGKRAVYLL
                     PIYPALALLLAVWCHRQTSLGSLRTWCLRVLSVVAVVSALVMLFVALGDLWTHDPSRL
                     FAPIETMLKPKDRANFLVIVNQIAGFGWKFDVAALGSAVLWSVFGYYLWRGRMVAAGG
                     ALVALSIILAFFSRGLVEKTIAETKSYRDFMVEVNRLVSPSEKLVLAGGFNGDPVAFY
                     RSGILEVAERPSGDGSAQVFAGNGYIVTTEKNWSRLKQAQPELTPLLRSRGTGPEGDA
                     PLVLLRAQVR"
     gene            complement(9481..10461)
                     /locus_tag="VMZ02_09020"
     CDS             complement(9481..10461)
                     /locus_tag="VMZ02_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72116.1"
                     /translation="MKANKSLIGILAVLVCSVGVQIAGAQNLEKIRLGYSGTGINNYV
                     LEMGKRTGIFKKNGVDLEVVYVNSGSLLSQALIAGTFDLSFSQGSEAMMAKIRGADVR
                     IAAVIANRFNHVYLSAPSITSIKQLKGKKVAVSRFGSGSHFQTNLALKEGGLDPEKDV
                     TVLQIGNSGARITAILSGVVDGTIMAADFVPRAKREGFNVLADLADTKIEYPFLSLNF
                     VGTYLDRNQKNVKALIKSMSESIRALQSDATTAKAVIRTSLRTDDVATIDYALARSVQ
                     VLDRRPFPTPGGIQTVLDELGKEAKTKTWKFDDFVDLRALRELEKEGFFK"
     gene            10617..11543
                     /locus_tag="VMZ02_09025"
     CDS             10617..11543
                     /locus_tag="VMZ02_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HUR72117.1"
                     /translation="MDLGRRSVIKAGLAVGASLLLPAQVSAAQSQPLIQKKIPSSDEL
                     IPIIGIGTARRYEEVKSDSEKVPLRETIRQFQTSGGTVIDSSPSYGTAEAVVGEIVEG
                     LKIRDSLFLATKVSLRKGGREEGMAQIEASFKRLRTNKIDLLAVHNLLDTDMQLKTIR
                     ELKAAGRVRYVGITTSFDNQYGGFEQVMKKETLDFIQVDYALDNRDAGDRLIPLAGDR
                     GMAVMINLPFGRGRLFNAVQGKKLPEWTAEFDCASWAQFFLKYIVAHPAITCAVPGMA
                     RAEYVVDNLGAARGRLPDAAMRRRMEQFIDNA"
     gene            11637..12890
                     /locus_tag="VMZ02_09030"
     CDS             11637..12890
                     /locus_tag="VMZ02_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72118.1"
                     /translation="MIDVRPEELRALGWSWLYIISLFVSYYILRPIRDEMGVAGGIEN
                     LPWLFTGTLLGMIAVNPPFAALVKTLPRVKFIGISYGFFAFNLLCFAVLLGMASGEQN
                     IWIGRVFFVWTSIFNLFVVSVFWALMVDVFDTDQSKRLFGFIAAGATIGGIVGSSVTA
                     SLAKQVPATYLLLASAILLEFAVLCVGRLSRLSSSLTARPARPSEEPPIGGSIWSGLS
                     HATQSPYLVNVSLFLLLFAVTSTFLYFQQAEIARQYFADRGARTAFFAGVDLWVNVLT
                     LGAQLFVTARTIKRIGVALTLALLPLLSVFGFSVLVWMPTIAALVVFQVLRRAGNFAF
                     ARPTREVLFTVVPREDKYKAKSFIDTVIYRLGDQVGAWSYAGLGALGLGMTGVSIVAV
                     PISLLWLINSLWLGGKQRQLESANE"
     gene            complement(12960..13496)
                     /locus_tag="VMZ02_09035"
     CDS             complement(12960..13496)
                     /locus_tag="VMZ02_09035"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HUR72119.1"
                     /translation="MPDSNHLILTAVGPDRVGLVEKIAAFIARHDCNIEDSKMAVFCG
                     EFAVIVLITGEGNNLVKIGRDYREIESETGLTIAIKTPTMRGTAESFLPYKLTASCMD
                     HPGIVYQISTILSGMGINIESMETKTYAAPISGTPIFQLEADLAVPTRTNVAELRQRF
                     GEIQRQENIDIDLTPAKN"
BASE COUNT         2900 a         3529 c         3998 g         3174 t
ORIGIN      
        1 ctcgtccttg aagaagagcc ggccttccgg gatcgcccat acttccccgg taaacttgag
       61 atcgctgagg taatggagaa aatcatctgt gaaaaacccc tgcgcggcga gataatcgag
      121 ggcggaatta ttgaaagaga aggtttctag ataatcgagg acgtcacgca aacctgcggc
      181 gacgaagtag ccgcggttga gtggataaga gcgtatgaac agactgaatg tcgccgcatc
      241 gagccgttgc tcgcggaagt acgcttgcgc catggtgagc tggtaaagat cggtgagtaa
      301 ggttaagttc tgcttgcctg gcataaactg gtaagccgat cgctaggcaa ctcaatagcc
      361 tcttcttggc ttactggcaa gttgctcctg ttgatttgtt ccgaccgttt tgttaacaat
      421 tttctaaagg ttccaggtaa ttagccatca accaggaggc attattcatg tttaccgata
      481 aggtggattt gaaggatttc gacaagcaag atccggaata tcaagatctg ctccgtcggg
      541 ttttgtcgat tcaggcggat tgtgagatcg gcggtcccaa tctttatgtc aaagacattt
      601 tgccaaacgc gccgaccaag ctcgatcaat tgatcgtagc gcgcaccgcg gccgaagaga
      661 tggaccattt tcgcaagatc gccaaactag ccggcgatat cggcgtcgat gtttccttcg
      721 ttctcagccg ccccaacaaa gatcgctatg tcgaggcgtt tcgcggggaa atcaaaactt
      781 gggaagacta cgccgtcttc ggattcttga ttgaccgcgt cggccgcttt cagctcgaag
      841 agtttatcgg ctgtacttat ctgccgttgg cccgcgtggt tgaagatccc gagatgatcc
      901 gcgaggaaga agggcatatc gatttcggcg ttaacgcaag tgcggagtgg gcggccaaag
      961 gcggtgagac gaaggagaga attcagaagg cggtcaacta ttggtacgtc aagggactgg
     1021 acatgtttgg caacagtaag tcgtaccgtt ccgagcgtta catgcactgg gggttgaagc
     1081 gccgtaccaa tgcggaagct cgcgcgttat acaaagaaga aatcgatggc ttgctcagaa
     1141 aaatggatct agatattcct gatccaaacc agggccggct ctatacttag ttcgaaggaa
     1201 tttactcgca tggaagcagc accaaacact gatgcgttgg accagcagtt caaagaattc
     1261 atccaggctt tgaaaaaagc cgtgggcaca ggcatacccc atctcaaaga cgatcccaaa
     1321 atcgttgacc aggcaatcgt ggactgtaag gagatcctgg atgtcgtgat ggagggaaat
     1381 gtgaaggagt ggatctccta aacattcgtt tgtcactcat ctccgacgag aaagcttcat
     1441 gacaaggcgc ctggatccaa ccggcgcctt tgtcgtattt acaaccggta tattcaggga
     1501 cgggagaacg ggacattgaa cgtcatcgta tgcggaaaag tcattcccgc gagcacggtc
     1561 accattgaga tcgatgcgaa taccaaacgg ctggttcgca agggcgtgcc gcatgagctc
     1621 gacccggccg cggcgagcgc cgtcgaagaa ggattgcgcc tgacggaaaa gctcggtggc
     1681 acagtagtac tggttacgat ggggaccagc gatgccacca tcggtatccg caatgctttg
     1741 gccatgggcg tgacttcggc ggtgcatatt ctcgacgatg tggtggccgg ctccgatact
     1801 cttggcacag ccaagctgct cgccgccgcg attaagaaac aggattacga tctggttatc
     1861 tgcgcaacgg aaagcggcga tagctactcc gggattgtcc atggtcagat cgcccagctg
     1921 cttggattgc cgccgcttac cttcgccaag gaaattactg tcgatggcgg taatatcacc
     1981 atcaagcgcc agagtgagtc ggggttcgag atcgtcgaat cgaccttgcc agcattggtg
     2041 gcgtgcagca gcggcatcaa cgaaccgcgc tacccgcagt tgaagggcat catggcggcg
     2101 aagaaaaaag agatcaaagt ttatagcgcc gcagatctcg gtctatcgcc ggagcaagtc
     2161 ggtgaaaagg gtgcgcgcga aaaagttctc accatcggcc gaccgcccaa gcggcaagcc
     2221 ggcaaagcgg taaccgatga aggggaaggc ggcaagcaga tcgcggattt tcttgctgaa
     2281 atcaaggtga tctagtggcg gagacaatct gggtatacgc ggaaatcagc gaagacaaaa
     2341 tcacgaccac gtcgttggag atgttggcca aggctgccga agtgggcgcg gccgaggcga
     2401 ttctcctcgg cccggccccc gacgatgccg tccagacctt ggcgaatcat ggcgccagca
     2461 agatataccg atcgaccgac gcggtttaca aagattatct gacattgccg gcggcggaaa
     2521 ccgttgcggc attgattcaa aagcacaatc cctcggtgat gttgtttgcg tcgagttatg
     2581 ctgggcgtga tttggtcgcc aacttgagtg ctcggctcga ttgcggcgcg attaccgatg
     2641 tgggtgattt cgaactcaaa gacggcagtg tcgttgccac cattcccgcc ctcggagcga
     2701 gttatcaaaa taccagcaca ttactcactt caggaaccaa acttttagtc gtccggccga
     2761 agtcttttga acccaaagaa aatccacaag ctctcgctgt ggaagaagtg cccgcggcag
     2821 ctagcgctga tttgcgcaaa gttcacatga aagagcgagt ggtagtcaaa cgcgagggcc
     2881 catcgctgga aggcgccaag atcgttattt ccggaggtcg cggactcaag ggcgaagagc
     2941 agttcagcat actcaaggac ctcgccgacg tgctcggcgg cgccgttggc gcgagccgcg
     3001 ccgcagtgga tgccggctgg gtgccctacg ccatgcagat cgggcagacc ggcaagaccg
     3061 tcaaaccgga tatctatttt gccgtgggca tctccggcgc cgtgcaacat ctatccggca
     3121 tgaagaccgc caagtacatc atcaccatca acaaagatcc cgacgcgccg atcttccagt
     3181 actctgattt cggcgtcgtc ggcgatcttt ttaaaatcgt gccgcaatta atcgaagagc
     3241 tgaagaagcg caaaggcgcg tagccgtcgg atcttaatag ccgcaaagaa cgcaaacggc
     3301 ggatgtaggg ggcgggtctc agacccgccc tttttgtgtt cgtggattgc gagagtaggg
     3361 cggcgatttt cgcgttggcg caaggtcgtt gcgccgccat gttgctaccg gtcattcctc
     3421 accctcgtgc ccaggtccct gaatccgtcg acgctcaatt gccttatcat ccgttattcc
     3481 ggcggacgcc ggaatccagg tttcctcaac cgtagagaag tatctctcga tcaaagatac
     3541 tggatgccgg cttgcgccgc atgacggatc ttcacttggc ctgatggcgc taacctttaa
     3601 gcacaccagt agctttcgcc gtgtgcgttg caatggcgtc catcagcacc ggagacaggc
     3661 agtcataagg ctccaacttc aattccttca gccgcgctct caccgcgccc atgttggctg
     3721 gatcggcgcc ggactcgatg atcgatgaca cgaaggcagc gaattctggc ggcgcccaac
     3781 cgggttccga cgagagttcg gtgtgaatga aatcgaagcc gtaaaaagga tgtttggtgt
     3841 tgtcgatgcg gccgtacatg tgcacaccac agccgctgca cgcgtagcgt ttgatcgccg
     3901 cgttggcatc gacgactttc agcttgtctc cattggctgt taccgagaga ttatcccggc
     3961 tgaccacggc gacttgtgag aacagcgcgc cggccggctt ccaacacttg gtgcagccgc
     4021 agacgtggtt gtgcgcgcat tggcctttga tcgcgaccgc gacttttttg tcggcgcagc
     4081 ggcagagtaa actgccgccg gcgaagttgg ctgcgccagg ctttatgcct tgatcgaccg
     4141 ctggatgaat cgagagcgga gttgccatat taaattcctc cttgccaagt cttgatcggc
     4201 tagtcttaac gcaacagagt agacacaggc aagtctactc ctgctagtta agttgacgat
     4261 tgcgtcggcg cgcagtagta agagcaattg atcggggcgt gatcagtaaa agtctcgccg
     4321 ccgtctgcgg agagtcaatt atgaaaagat cttttccggt tatcgatgcc gacggtcaca
     4381 ttctcgaaaa ggatcatgag ttgcgcgaat ttcttccgcc cgaatttcgc gccgtgccgg
     4441 gaaagcgcga gtatccgctg ttttcctggg acggctgggc gcgcggcgcg ctgtcgccga
     4501 ccaaacgcga gcatccaagc gtcgagcttt ggctgcgttt tctcgatgcc accgagatcg
     4561 ccctcaccgt tctttatccc aacgagggtt tgaacatcgg cttgcagcgc gatcgcgatt
     4621 gggcggtcgg cttggcgcgc gcgtacaacg actggctgca tcatgatttc ttgagcgtca
     4681 gtccgcggtt tcaagctgta gcgctgttgg cgcctcagga gccgcaggaa gcggcaaaag
     4741 aattggcgcg cgcggtgcac gagcggggaa tgattgccgg catgttgccc ggggtaatga
     4801 gcccaatgaa gggactgggt ttgccggagt tcgacccgat ttatgcgatg gcggaaaaac
     4861 tcgacgtgcc tctcgctgtc catagcggcc cggcggtgga gctgggcttg gaacatatgc
     4921 aatccttcgc cggcgtttat ccgctgagtc atccctttgc gcagatgcag cagctaacca
     4981 gcatgatgat ctacggcgta ttcgagcgct ttcccaagct gcgtgtcgcg tttcttgaat
     5041 ccggctgcgg ctgggtgccc tacatggcag atcgtttgga tgagagctgg gagcatcggc
     5101 gcaatcgctg gccgcacccg gccaagcgtt cgccgagctc gattatgagc ggcggcaatc
     5161 tgtactacgc ttgtgaacga gaggaacgca ctttggcgac cgttgccggc ataatcggtg
     5221 cgggacagtt gctctgggcg tccgattttc cccatgagcg gccgtgggat gagttcagcg
     5281 gcgatatcga tacgctggtc aaccggaaag atttgccgga gtcactgcca cgcaaaattc
     5341 tattcgacaa tccccggcgg ttttataatt taaaagtgga tgggtaattt tctttcacgg
     5401 gttcaaggtt caagagttca atataagaat ccggatctga ccttgaactt ttaacttttg
     5461 aaccttgaac agtcttcggt aaaatctgga gtttagcgat gcgcgtactt atcaccggcg
     5521 ccggcctgat cggcacccac acggccaagg aattaatcga tcgccgcgat gatgtgacgt
     5581 ttttcgattt tgcgccgcgg ccggattata tccggcatgt gaccggcaac gatgtgccgg
     5641 tgttgcgcgg tgacattcgc gatttggcgg ctctgatcga tgcatgtcag acagtccgcc
     5701 cggattgcgt gattcatttg gcggcgtcgg tgggcgaggc aaatatcaac aacgtctacg
     5761 ccggctttca aattaattta gtcgcggcca tcaatgtcgc cgaggcggtg cggttggcgg
     5821 gggtgcgcaa gctggtgcac gccagcacgc aggcggtcta ttccggcgaa gatccgaaaa
     5881 cgttgctgcg cgaggactcg cccatcgact gccgcgagcg ggtatacaac gcgtcgaaga
     5941 ttggttgcga acatgtgctg cgcacttacg cggcaaaaca caatatagag ttggcgctgc
     6001 tgcgcttcgc gggcgtgtac ggctattatt ccgtggccgg tggtcccggt gtggcggtgc
     6061 agcaagcggt ctgggatgcc atggctggga gagccgccgt actcaacgtg tatgaggcag
     6121 tggattttat ctatgtcaaa gacttggcca acggcatcgc cttggcggtg cacacggcgt
     6181 cgctgccgca caacgtctat aacctgggca gcggcaggtt gacgactgtg gcagatgtcg
     6241 aatcggcgct cgcggcaatc tttcctggca taaacatgaa acgaggcaaa cttacgccgg
     6301 cgcgtccgcg catggatatc atcagggcgg agaccgagtt gggatataaa ccggagtata
     6361 agttggaaac cggattgcgc gattacgtca cggagttgaa gaacgggagt tgaccgcaaa
     6421 cagcacaact gtcacaaagc gaggttattc ggtttggaaa ttgcccgcaa cgagtgcaaa
     6481 cgaaacaata cgaggagagg aaatgaaaat cgctgtcatc gacgattatc agaacgcttt
     6541 caaaacttta aaatgctttc ccaagctggc cggccatgag gtggtcgtgt ttacagatac
     6601 cgcgaatgac gtggccagtt tggcggaaaa attgaaagac gcagatgcgg tggtattgac
     6661 gcagcagcga tcttatatcc cacgcgagct gatcgaaaaa ttgcctaagc taaaactaat
     6721 cggtcagacc gggcgcgcgg cgacccatgt cgatctgcag gcgtgcactg agaagggaat
     6781 tatagtatcg gcgggcggtt caggaaatcc caacgccaca gcggaactca catggggact
     6841 gatcctgtca tcgctgcgca gtctgccgcg tgaagtgacg cgtctcagag agggccaatg
     6901 gcaatcgacg ctaggcactg gtgtgagcgg caagaccatc ggtgtctacg cctacggcaa
     6961 gatcggcagt atcgttgcag cggtgggaaa ggccttcggg gcgcgggtgg tttgttgggg
     7021 gcgcgagggt tcgaccggtc gagcgaaggc tgccggcttc gaagttgcca atagccgcga
     7081 ggagtttttt gccgaagccg atatcatcag cttacatttg ccattgaacc aagatacccg
     7141 cggcattgtg aacgcaggcg atttggcgcg catgaaaccc agcgctctac tggtcaacgc
     7201 cagccgctcc ggcttaatcg ccaagggcgc tttggtcgag gcattgaatg ctgggcggcc
     7261 gggcttcgcc gccgtcgacg tttacgacaa agagccggtg ggcgcagatg agccattgat
     7321 taagatggcc aatgtcacct gcacgccgca tctaggttat gttacgcgcg aaagttacga
     7381 ggagtatttc gctgtggtgg tcgatgacat cgtcagcttc gcggcgggca agccgagtca
     7441 tgtgctcaat cccgaggcgc tgggaaagaa ataatctgcc ggcacactcg gcagtccata
     7501 tagaccgaca cggaaccacg gcgaaatatt ttagcggaac ttgttggcgt cgggctatga
     7561 ggaactgcgc tgtgtcgacc gtgtacgggc aatgcgatct tttatggcgt gcgacaaatc
     7621 ctgctgaata ttccagctaa atgcgccgag cggcggcgaa cataaccgtg tgtcaaggaa
     7681 agctctgcgg cggcggataa gcctgccaag cgcaaaatgg tcacgcctca atcaaggaga
     7741 caaacaatgc cggcagccac tgagccgtgc gcggctatcg acggcatcca aggaaatcgc
     7801 tggctgcacg tttttttact gattgcgctc tgcggtgtgc tttactttcc atacctcggc
     7861 agcacgccgg ttttcgacaa aggcgaaccc cgagaagggc tcgcggtcca ggatatcgtc
     7921 cagcgcggcg agtggttgtt tccactcaag cgggcagcgg cgattccctc caaaccgccg
     7981 atgtttcatt ggtcggcggc cctggtctat caattcaccg gcgttctcga cgaggcgact
     8041 ctgcgtttac cctctgcagt ttacgctacc ctcggggtaa tttctctcta cctccttggc
     8101 gctaagctgt tcggcgcaca agtcgcgcta ttgggcggag cgattctcgc aacaacgatg
     8161 atttatcaga cccaggccgt caacgcgcgc gtcgatatga cgttgtgctt cttcgtcacg
     8221 ctcagcctga tactgttcta cagcatttac cgtggtttat tgacccatcc gctatggccc
     8281 tatgcgatgt atcttttggt cggcttggcg acactttcca agggaccgct cggcttggtc
     8341 ttgccggggc tggtgatcgc ggtttttctc gcgctcaaac gcaattggca gcagtttttg
     8401 agatttgctt ttcatccggg cgtattgatc gccgtgattc ttgtcttggg ctggtatggt
     8461 ctagcgatca ctcgcggcgg cgaaggtttc gtcgatcgcc agctactgtc cgagaatctc
     8521 ggccgctttt tcggcggctc gggacatagc catccgcttt actattattt gggttatctg
     8581 ttcagcctag ccattccatg gggtcttttt tatccattga tgttttggga ttgggccgaa
     8641 caccgcggca gcgccgaagg cgacgatgtg cggtttctgc aaacctggtt cgccgccatg
     8701 gtgatttttt tctcgttgtc gtccggcaaa cgggcggtct acctcttgcc tatctaccca
     8761 gcattggcac tgctgttggc agtgtggtgc catcggcaaa caagtttagg ttctttgcgc
     8821 acctggtgcc tccgagtcct ttcagtggtc gctgtcgtca gtgctctggt tatgctgttc
     8881 gtagctctcg gtgatctctg gactcatgat ccgtcaaggc tattcgcccc gatcgagaca
     8941 atgctcaaac ccaaagaccg cgccaacttt ctcgtcatcg tcaatcagat tgccggcttc
     9001 ggctggaagt tcgatgtcgc cgcattgggg tcggcggttc tttggtcggt ttttggctat
     9061 tatctgtggc gtggccgtat ggtcgcggcc ggcggcgcgc tggtcgcgct ttccattata
     9121 ctggcgtttt tcagccgcgg cttggtggag aaaacgatcg ccgagacgaa gagctaccgg
     9181 gatttcatgg tcgaagtgaa ccgtctggtc tcgccgtcgg agaagttggt tttggctggc
     9241 ggcttcaatg gtgacccggt ggcgttctat cgcagcggca tcttagaggt agccgaacgc
     9301 ccttcgggcg atggatcggc tcaagtcttc gctggtaatg gctacatcgt caccacggag
     9361 aagaactgga gccgactcaa gcaagcgcaa cccgaattga cgccgttgct gcgcagccgc
     9421 ggcaccggac ccgaaggcga cgctccattg gtgctgttgc gcgctcaggt gcgctagcga
     9481 ttatttaaaa aacccttctt tttccaactc acgcaaagct cgcagatcga caaagtcgtc
     9541 gaacttccag gtcttggtct tcgcttcttt acccagctca tcaagcacgg tttgaatgcc
     9601 gccgggtgtc ggaaaaggcc gccggtccaa cacctgtacg ctgcgcgcca gtgcatagtc
     9661 tatggtcgcc acatcgtcgg tgcgcagtga tgtccggatg accgcttttg cggtggtcgc
     9721 gtcgctttgc aaagcgcgga tcgactcgga catgcttttg attagtgcct tgacgttttt
     9781 ttgattgcgg tcgaggtatg tgccgacgaa gttaagcgac aaaaacggat actcgatctt
     9841 ggtatcggcg agatcggcga gtacgttgaa tccttcgcgc ttggctcgcg gcacgaagtc
     9901 ggccgccatg atcgtgccgt cgacgacgcc gctgaggatc gccgttatgc gcgcgccgga
     9961 attgccgatc tgtaagaccg taacatcctt ttccggatcg aggccgccct ctttgagagc
    10021 caaattagtc tgaaagtggg acccggagcc gaagcggctg acggcgactt tcttgccctt
    10081 gagttgcttg atcgatgtga tcgaaggcgc ggatagatag acatgattga agcgattggc
    10141 gattaccgcg gcgatacgca cgtcagcgcc gcgtattttc gccatcatgg cttcggatcc
    10201 ctgagaaaag gacagatcaa aagtgccggc gatcagcgct tggctcagca gcgaaccgct
    10261 attgacgtaa accacttcga gatcgacacc gtttttcttg aaaatgcccg tacgtttgcc
    10321 catttcgagg acatagttgt tgatacccgt gccactgtaa ccgagacgaa ttttttcaag
    10381 attctgtgcc cccgcgattt gcaccccaac cgagcacacg agaaccgcca atatcccgat
    10441 cagtgattta ttagccttca ttccgcgctt ctcctttcgg tgaacggaaa tcaccgcatc
    10501 gtaattctga cgtactgtaa gcacaagagg aaaaaccggt tcattgccga agagcgattg
    10561 tcttgcgagg tctagcaact ggtgttacat ggtgctgcga gcgagaagga gagcgtatgg
    10621 atttaggtcg taggagcgtc atcaaagccg gattggcagt gggagcgtcc ttgctgctgc
    10681 cagcgcaagt atcagcggca cagagccaac cgctgattca gaaaaaaatc ccgtcatcgg
    10741 acgaattgat tcccatcatc ggcatcggca ccgcacggcg ctacgaggaa gtgaaaagcg
    10801 atagcgagaa agtgccgctg cgcgagacga tccggcagtt tcaaacttcc ggcggcacgg
    10861 tgatcgacag ctcgccatcc tacggcacgg ccgaggcggt cgtcggcgaa atagtcgagg
    10921 ggttgaagat tcgcgactcg ctgtttcttg ccaccaaggt gagcctgcgc aaaggtggaa
    10981 gggaagaggg catggcgcag atcgaagcgt cgtttaaacg attgcgtacc aacaaaatcg
    11041 atctgctcgc cgtgcataac ctgcttgata ccgacatgca gctgaaaacg atacgcgagc
    11101 taaaggctgc gggacgggtg cgctatgtcg gcatcactac gtcgttcgac aaccaatacg
    11161 gcggctttga gcaggtgatg aaaaaggaga ccctggattt tatccaagtc gattacgccc
    11221 tcgataaccg tgacgccggt gatcggttga ttccgcttgc cggcgatcgt ggcatggccg
    11281 taatgatcaa cctacccttc ggccgcggtc ggctcttcaa cgcggttcaa ggcaaaaagt
    11341 tgcccgagtg gacggcggaa tttgactgcg ccagttgggc gcaatttttt ctgaaatata
    11401 ttgttgcgca tccggccatc acttgtgccg tgcccggcat ggcccgggcg gaatacgtag
    11461 tcgataatct cggcgcggcg cgcggcagat tgcccgatgc ggcgatgcgc aggcgtatgg
    11521 agcagtttat cgataatgcg taggcggtca cccatgagag tgagggatta cgagtgggga
    11581 acgatgctaa tccacaagcg ggaactcagc gtgttctcta tggattccta cggcacgtga
    11641 tcgatgtccg gcctgaggag ttgcgcgcgc tcgggtggtc gtggctttat attatttcgc
    11701 tttttgtttc ttactatatt ctcaggccga tccgcgatga gatgggtgtc gccggcggga
    11761 tcgaaaatct gccgtggctt ttcaccggca cgctgctcgg gatgatcgcg gtcaatccgc
    11821 cattcgccgc tttggtgaag acgctgccgc gggtcaagtt catcggaatc agctatggtt
    11881 ttttcgcctt caatcttttg tgtttcgccg tgcttctggg gatggcgtcg ggtgagcaga
    11941 atatctggat cggccgggtg ttttttgtct ggacgtcaat tttcaatctc tttgtcgtct
    12001 cggtcttttg ggcgctcatg gtcgacgtct ttgataccga ccagagcaaa cggctgttcg
    12061 gttttatcgc tgccggggcg accatcgggg gtattgtcgg ttccagcgta actgcgtcgc
    12121 ttgccaagca ggtgccagcg acctatttgt tgttggcttc ggcaatcttg ctggagtttg
    12181 cagtcctttg cgtcggccgg ttgtcacgtc tatcttcgag tttgacggcg cggccggctc
    12241 gcccatcgga agagccaccg atcggcggtt cgatttggag cggtttgtcg catgcgacgc
    12301 aatcgcctta tctcgtgaac gtcagtttat ttctcttgct gttcgcggtt acctcgacgt
    12361 tcctgtattt ccagcaagcg gaaatcgcca gacaatattt cgccgatcgc ggcgcgcgga
    12421 cggcgttttt tgccggtgtc gatctgtggg tgaatgtttt aactcttggt gcgcagttgt
    12481 tcgtaaccgc tagaacgatc aaacgcattg gcgtagcgct gaccttggcg ttgctgccgt
    12541 tattaagtgt cttcggcttt agcgtcttgg tttggatgcc gacaatcgct gcgctcgtgg
    12601 tttttcaagt tctccgacgc gccggcaatt ttgcctttgc ccggccgact cgtgaagttt
    12661 tgttcacggt tgtgccgcgc gaggataaat acaaagccaa gagttttatc gataccgtaa
    12721 tctatcgatt aggcgatcag gtcggtgcct ggtcctatgc cggattgggg gctctcgggc
    12781 tagggatgac tggagtgtcg atagtcgccg tgccgatttc gctgctttgg ctgattaata
    12841 gtttatggct gggcgggaag cagcgccaat tggaatccgc caacgagtag ctaagcaatt
    12901 ttatttgcgt tggcggggaa caaaaagatt gaaatggtcg aagacagtcg gactttctat
    12961 cagttcttag ctggcgtgag gtcaatgtcg atattttcct gccgctgaat ctcgccgaag
    13021 cgctggcgta actcggcgac attggttctt gtcggcaccg ccaaatcggc ttctagttgg
    13081 aaaattggcg tgccgctgat cggcgcagcg taagtcttgg tctccatcga ttcgatgttt
    13141 atgcccatgc cgctcaatat ggtgctgatc tggtagacga tgcccgggtg gtccatgcat
    13201 gacgcggtga gtttgtaggg caagaacgat tcggcggtgc cgcgcatcgt tggcgtcttg
    13261 atcgcaatgg tcaaaccggt ttcgctttcg atctcgcgat aatcgcgacc gatctttacc
    13321 agattgttgc cctcaccggt gatcaagaca atgacagcga actcgccgca aaataccgcc
    13381 atcttgctgt cttcgatatt gcagtcgtga cgggcgatga aggcggcgat cttttcgacc
    13441 agtccgacgc gatcagggcc gacggcggtg agaatgagat ggttggagtc tggcatgatt
    13501 taacgctaat aaattcgttc aaaccgttca aatcgttcaa gccgttcaaa acgtttccag
    13561 aactcaccac cagtggcgca aacggtttcg gaaattctga a
//
LOCUS       DATLCH010000306         3477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6376151, whole genome shotgun sequence.
ACCESSION   DATLCH010000306 DATLCH010000000
VERSION     DATLCH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6376151"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VMZ02_09040"
     CDS             complement(<1..1164)
                     /locus_tag="VMZ02_09040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72120.1"
                     /translation="MDEEVIEPTAAPITDNPVSARFRTFASLRHLDFRYLWTGTVMMS
                     AGQWVQQVTLGWLLYDLTGNSVLLGALNGLRALPFLVTGPMAGVAADRMDRRKLLIGT
                     QWVLIATAILFGALVASPYLHVSHIFIFTLITGVAWTITEPVRMSLIPSAVPKEELAN
                     AVALNSGGFNMMKVLGPALGGALIAWFGAAENFFVQGVAYAGVLVMIYRMRVPPQRAD
                     ARHTSAMANLKEGFAYVWSTPAVLALMTLAYVPRIFAVPYQTLMPVFQKDVLKIGPEG
                     LGLLMAAPGVGAVIAILTIASMGNRIKRQGRFLVGSIIVLGAAITVFSQITWFPLALV
                     TLVIVGIFQMFFLASTATMLQMIVPDELRGRVLSLYMLDRGFMPLGALFAGTSA"
     gene            1350..2321
                     /locus_tag="VMZ02_09045"
     CDS             1350..2321
                     /locus_tag="VMZ02_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72121.1"
                     /translation="MKHIRWLVLIVLLLPSSVPAQERVKFPVGVSSKVLGYGTLWAAW
                     RRGYFEREGLDVDVVLMRGTAPAVQALIGNSISAALLANDGPITAVEQGADLVMVASG
                     SKITHMLMGAKNYKTYEDLRGATIGSSTLTSGTTFVLRRALKLKGLEYPRDYKLLSVG
                     GTTEAFLAMNAGQTAASMMAVPYSFRAQEMGFNLIGRMVDVFPNYLLSAFSVRRSWAE
                     KNRPVVVRFLKAVVRAKKWFEQDRKTAIEYLAKEFQLTPALAEKGLDYYLQAPAWHPE
                     LEIEMDGLKTVVDIYAEQNNLKGPIPNAEKYVDQSYLRQALKEIGLR"
     gene            2379..3359
                     /locus_tag="VMZ02_09050"
     CDS             2379..3359
                     /locus_tag="VMZ02_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72122.1"
                     /translation="MNIDLHSHFFPIDALQDPGKFANRAPKLIVKDGKLSVTSQIGAR
                     PGLTAGAYDPVARIKALDDMKIDMQAISPSPILLFYWEDAAVAAHFSRRQNEAIQDVV
                     KKHPDRFIGFGSVPLQSVPESIAIAREAKSMGLKGLEIGNAVGDKPLDDPSFEPFFQT
                     AQELELLLFVHPLEGGLDADDPLSPILGNVLQFTFRTTLMIERLILKGLFEKYPNLRL
                     CLSHGGGLLAFNIGRLDHSYGLRPDLQKQLPCAPSEYLKRMYFDTIVHSVTALDYLIR
                     VVGADRVVIGTDYPMAMGDVNSVDKVMQLQISETERRSILGGNAVRELKL"
BASE COUNT          797 a          914 c         1012 g          754 t
ORIGIN      
        1 cgcggaggtg ccggcgaaca gggcgccaag cggcatgaag ccacgatcga gcatgtagag
       61 acttagcacc cggccgcgca gttcgtcggg cacgatcatt tgcagcatag tggcggtgct
      121 ggcgagaaaa aacatttgaa aaatgccgac gatgaccagc gtcaccaggg cgagaggaaa
      181 ccaggtgatc tgcgagaaaa cggtgatcgc cgcgccgagc acgatgatgc tgccgactaa
      241 aaaccgtccc tgccgcttaa tccgattgcc catcgaggcg atggtcaata tggcgataac
      301 cgcacccacg cctggagccg ccatcagcag tcccaatcct tccggaccga tcttgagcac
      361 gtctttctga aacaccggca tgagagtttg ataaggcacc gcgaagattc gtggcacata
      421 ggccaacgtc ataagcgcca aaaccgccgg cgtcgaccag acataggcaa acccttcttt
      481 gagatttgcc atggctgacg tgtgtctggc atctgcacgc tggggcggca cgcgcatacg
      541 atagatcatt accagcacgc cggcataggc gaccccttgg acgaaaaaat tttccgcggc
      601 accgaaccaa gcgatcagcg cgccgcccag cgccggtccg agcactttca tcatattgaa
      661 gccgccggag ttgagcgcca cggcattggc gagttcctct ttgggaacgg cgctgggaat
      721 caaactcata cgcaccggct cggtgatcgt ccaggcgacg ccggtgatca aagtgaaaat
      781 aaaaatatgc gagacatgca aatacggcga cgccaccaaa gcgccaaata aaatcgcggt
      841 ggcgatcaat acccactgag tgccgattaa aagtttgcgg cggtccatgc gatcagccgc
      901 aacgcctgcc atcgggccgg tgaccagaaa gggcaacgcg cgcagaccat tgagcgcacc
      961 gaggagaacg gaattgccgg tgagatcgta gagcagccag ccgagtgtca cctgctgcac
     1021 ccattggccg gcgctcatca tcaccgtgcc ggtccacaaa tagcgaaagt ctaaatggcg
     1081 cagcgaagcg aaagtgcgga agcgggccga aacgggattg tcagtgatcg gtgctgcagt
     1141 tggttctatg acttcttcgt ccattgggct ctaggaaagc attggcgtgc tgatactttc
     1201 ttctttgtat caagattccg ccgcggttac gaaccgcgaa gcatcggcga gacaaatttg
     1261 agtttgcaac tggattcgaa catgttacaa ggcagccggt ttatcgatct gactcgattc
     1321 ccgtcatcga tgtggataaa ggagttgcga tgaaacacat tcgctggcta gtgttgatcg
     1381 ttttgttgtt gccatcgtca gtccctgccc aagagcgcgt caaatttccg gttggggttt
     1441 cgtccaaagt tctcggctac ggcactctgt gggcggcttg gcggcgcgga tatttcgagc
     1501 gcgaggggct cgacgtcgat gtcgtactca tgcgcgggac tgcgccggcc gtgcaagcct
     1561 tgatcggtaa ctccatttct gcggcgctgc tcgccaacga cggacctatc acggcggtgg
     1621 agcagggagc ggatctggtg atggtcgcca gcggcagtaa gattactcat atgctcatgg
     1681 gcgcgaagaa ttataaaacc tatgaagacc tgcgcggcgc taccatcggc tcttcaacct
     1741 taacctcggg cacaactttt gtgctccgtc gcgccctgaa gctcaagggt ttggaatatc
     1801 cgcgcgatta caaactgctc agcgtcggcg gcaccaccga agcattcttg gcgatgaatg
     1861 ccggccaaac cgccgcgtcg atgatggcgg taccttattc gttccgcgcg caagaaatgg
     1921 gtttcaactt aatcggtcgc atggtcgacg tttttcccaa ctatctgctt tccgcgttca
     1981 gcgtgcgccg gagctgggct gagaagaatc ggccggtggt ggtgcgcttc ctcaaggccg
     2041 tcgtgcgggc gaagaaatgg ttcgagcagg accgcaagac cgcgatcgaa tacttggcga
     2101 aagaatttca gctgacaccg gcgctggcag aaaaggggct ggattattat cttcaagctc
     2161 ccgcttggca tccggaattg gagatcgaaa tggatggcct taaaaccgtc gtcgatattt
     2221 atgccgagca aaataatttg aaagggccga ttcccaacgc cgagaagtat gtcgatcaga
     2281 gttatttgcg ccaggcgtta aaggagattg gactacgatg accagagttg atttcaaatt
     2341 attaccgcgt cggcgccaga acgccgaggg aacttaacgt gaatatcgat ctgcattctc
     2401 atttttttcc gattgacgcg ttgcaggatc ccggcaaatt cgccaaccgc gcgcctaaat
     2461 taatcgtgaa ggacggtaag ctgtcggtga cttcgcagat cggcgcgcgg ccgggactaa
     2521 cggcaggggc ttacgatccg gtggcgcgca tcaaggcgct cgacgacatg aagatcgaca
     2581 tgcaggcaat ctcgccgtcg ccgatcctgt tattttattg ggaagatgcg gcggtggcgg
     2641 cgcatttttc gcgccgtcag aatgaagcga ttcaagacgt cgtcaaaaag catccggacc
     2701 gttttatcgg tttcggcagc gtgccgttgc aaagcgttcc cgagtccatc gccattgcca
     2761 gggaagccaa gagcatggga ctgaagggat tggagattgg caatgcggtc ggggacaagc
     2821 cgctcgacga tccgagtttc gagccattct ttcaaaccgc gcaggagctg gagcttctgc
     2881 tattcgtcca tccgttggaa ggcgggctcg atgccgacga cccgttatct ccgatactgg
     2941 gcaatgtttt gcagtttacc tttcgtacca cgttgatgat cgagcggctg atcctcaagg
     3001 gcttattcga aaaatatccc aatctgcgct tatgcctctc tcacggcggc ggcctcttgg
     3061 cattcaatat cgggcgattg gatcattcct atggattgcg cccggactta cagaaacagt
     3121 tgccgtgcgc gccttccgag tacttgaagc ggatgtactt cgacaccatc gttcattcgg
     3181 tgacggcact ggactatttg attcgagttg tcggcgccga ccgagtggtg atcggtacgg
     3241 attatccaat ggcgatgggc gatgtcaatt cggtggataa ggttatgcag ctgcaaatca
     3301 gcgagacgga gcgccgctcg atattgggtg gcaatgctgt gcgcgaactg aagttatgaa
     3361 tgaagatctt gaatgttgca tgacaattta cgcgtgtttc ctgatgttgt ccgtttcact
     3421 taaagcgcat acagggtcct aggattgtcg tcgagaatct tgcgcgcgaa ggacggg
//
LOCUS       DATLCH010000307         5000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10107106, whole genome shotgun sequence.
ACCESSION   DATLCH010000307 DATLCH010000000
VERSION     DATLCH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10107106"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /gene="ccsA"
                     /locus_tag="VMZ02_09055"
     CDS             complement(<1..1340)
                     /gene="ccsA"
                     /locus_tag="VMZ02_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HUR72123.1"
                     /translation="MTASLGHTAILLAFVAALIGIFSPIVAARSAGANFRAVARYSIL
                     GQFVLVTSAAVALIYGLVTTDFSIKYVAFNTTRATPIYYRVTGLWGALEGSLLLWEWL
                     LIIFAGIVARVYRNRHHDMMPWVIMIFSIVSAFFLGVIGFLSNPFDTISPIPLDGRGL
                     NPLLEDANMMSHPPLLYTGFVGLTVPFAFAMAALIVGKLDEAWIVSTRRWTIIAWFFL
                     TAGNLVGAWWSYHVLGWGGYWAWDPVENAAFMPWLPATAFLHSIQVQERRRMLKVWNL
                     SLIIVAFSLTIFGTFLTRSGIISSIHAFSSGPVGIFFLAFLAVILIGSFGLLAYRADL
                     LKEQPELDSMVSRESAFLLNNIVLVSALFTIFLGTIFPLISEAVAGAQVSVGAPYFNT
                     VTVPLFLFLVFLMGVGPIIAWRRASWDNLKRNFVWPAAASLAFGLFLFAWKIKE"
     gene            complement(1337..1780)
                     /gene="ccmE"
                     /locus_tag="VMZ02_09060"
     CDS             complement(1337..1780)
                     /gene="ccmE"
                     /locus_tag="VMZ02_09060"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HUR72124.1"
                     /translation="MTKAKRFLIGGAVILAALGYIIYGGMQQALVYFKMPSELRAEEK
                     SMHGKFVRMGGMVVKGSMEKDLQNLTYRFQITDGSTNFPVYFKGVPPDLFVEGKGAVV
                     EGRVENDGVFHATTIMAKHAEDYSPHADGKGASKSFVPAKEAGAK"
     gene            complement(1767..1922)
                     /locus_tag="VMZ02_09065"
     CDS             complement(1767..1922)
                     /locus_tag="VMZ02_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72125.1"
                     /translation="MGAWGYVSLAYGIVCGVILMYWFLLKRRYNAAEAELNRLRSSEA
                     AANHDKS"
     gene            complement(1922..2629)
                     /gene="ccsA"
                     /locus_tag="VMZ02_09070"
     CDS             complement(1922..2629)
                     /gene="ccsA"
                     /locus_tag="VMZ02_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HUR72126.1"
                     /translation="MERSTKTVKRRKGMLGVLALVAVVAGLCWSLLVAPPDAYQGEVQ
                     RIMYLHLPSILTAYLSYFLVFIGSGMYLWKRDNKDDLLAHAAAELGVLFTALTIIEGS
                     IWGKPTWGVWWTWDARLTLTAILLLIYAGYLMLRSSIEDEQRAASAAAILGILGFLDI
                     PLIHMSVYWWRTLHQPPSILRPDKAPWENVHPAMLTALTISFVGFLLLYFYLLSLRYR
                     VGEMRQEIKALRLERGV"
     gene            complement(2632..3309)
                     /locus_tag="VMZ02_09075"
     CDS             complement(2632..3309)
                     /locus_tag="VMZ02_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HUR72127.1"
                     /translation="MTLLRQAYLLLRKDLLLEWRRRDSLLTMFFFGTLLLFVFHFAFD
                     MPPDKVVELAPALLWLAFLFTGTLGLAQLFQAERDNHCLDALLLSPLDRGALFLAKSA
                     FNLILMLLVESVVIPLFWILFNLGSWNLLPQLFLVTLLGTLGFCVLGTITAAITLRAR
                     ARELLLPLVLFPLMIPVILATIRCMEMVLRTGSFGDAIAWLRLLIGFDVIFLTIGVLI
                     FDWVIES"
     gene            complement(3306..4013)
                     /gene="ccmA"
                     /locus_tag="VMZ02_09080"
     CDS             complement(3306..4013)
                     /gene="ccmA"
                     /locus_tag="VMZ02_09080"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HUR72128.1"
                     /translation="MRLTVKQLAKAYGYLWALRHFDLTLSPGEFVALLGPNGAGKTTL
                     LKLLAGLTPPTAGVIELDGRPLTGTAIAERGQIGLLAPAEHLYDQLTAVENLRFFTAL
                     YDKDSSASALDSALLQVGLSTRARDYVGNLSAGMKCRLSIAKWQLLDPGLLLLDEPYG
                     VLDGSGIDLLENFLKAHCARGHIVIMASHHVARALKLCDRALILHQGRLQFDQRKQTP
                     WPAFEHAFGDFLPRSGP"
     gene            4199..4873
                     /locus_tag="VMZ02_09085"
     CDS             4199..4873
                     /locus_tag="VMZ02_09085"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72129.1"
                     /translation="MNPVVTVTGFWDAFTTKIAVFLPNLLFAIAIILLGWVICNVIKR
                     VVVRVLKICQFDVLAERSGIKQILERGGVRQTASDIVGLLAFWFLFLIAIVTTLETLN
                     LSGATDTLHTIYLYIPKIVAALVTLILGLYFANFLESVTRTSCANAGLEAAPSLGRAA
                     YIGTSIFVIAGIFEILDIASEIVIWAFILVFGAVCLALALAFGLGGRDVAGRYLERWL
                     EQKKNE"
BASE COUNT         1241 a         1330 c         1438 g          991 t
ORIGIN      
        1 aactctttga tcttccaagc aaacaggaac agcccgaaag ccagagacgc tgcggcgggc
       61 caaacaaagt ttcgctttaa attgtcccag gaggcgcgcc gccaagcgat aattggcccg
      121 acgcccatca agaaaactaa aaagagaaac aacggcaccg tgacggtgtt gaagtacggc
      181 gcaccgacgc tgacctgcgc gccggcgacg gcttcggaga ttaaaggaaa aatcgtgccg
      241 agaaaaattg taaacagcgc cgacacgagg acgatattgt tgagcagaaa cgccgactcg
      301 cggctgacca tggagtcgag ttcgggttgt tctttcaaaa gatcggcgcg ataggcgagc
      361 aggccaaacg aaccaataag tatgaccgcc aaaaaagcga gaaaaaatat ccccaccggg
      421 ccggacgaaa atgcatgaat cgatgaaatg atgcctgagc gcgtcaaaaa agtgccgaag
      481 atagtcaagc tgaaggcgac gatgatcagc gacaggttcc atactttgag catgcgtcgg
      541 cgctcttgga cctgaatcga atgtaggaaa gcggtggccg gcaaccacgg cataaacgcg
      601 gcattctcca ccggatccca cgcccagtaa ccgccccaac cgagaacatg ataagaccac
      661 caggcgccga ccagattacc ggcggtgaga aaaaaccagg cgataatcgt ccagcggcgc
      721 gtgctgacga tccaagcttc atcgagtttt ccgacgatca gcgcggccat ggcaaaagcg
      781 aagggcacgg tcagaccgac aaagccggta tagagcagcg gcgggtgact catcatattg
      841 gcgtcttcca gcaatggatt taaaccgcgg ccgtcaagcg ggatcggcga gatggtgtca
      901 aacgggttgg acaaaaaacc aatgacgccg agaaagaatg ccgagacgat tgaaaaaatc
      961 atgatcaccc agggcatcat gtcatggtgc cggtttcgat agacccgggc gacgatgccg
     1021 gcgaagatga tcaggagcca ttcccacagt agaagcgagc cttcgagcgc accccacaaa
     1081 ccggtgacgc gatagtaaat cggcgtcgcg cgggtggtgt tgaaggcaac atatttgatg
     1141 gagaaatcgg tggtgaccaa gccatatatg agcgcgacgg cggcgctggt gacgaggacg
     1201 aactggccga gaatagaata acgcgctacc gcgcgaaaat ttgctcccgc cgagcgcgcc
     1261 gcgacgattg ggctaaagat cccgatcagc gctgctacga aagccaaaag gatcgcggta
     1321 tgacctaaac tcgctgtcac ttggcgccgg cttccttggc gggaacaaag ctcttgctcg
     1381 cgcctttgcc gtcggcatgt ggactatagt cttcggcgtg cttggccata atcgtcgtgg
     1441 cgtgaaacac gccgtcgttt tcgacgcgcc cttcgaccac ggcacccttg ccttcgacga
     1501 atagatccgg cggcacgcct ttgaaataga cggggaagtt ggtactgccg tcggtgattt
     1561 gaaatctgta agtgagattc tgcaaatctt tttccatcga gcctttgacc accatgccgc
     1621 ccatgcggac gaacttgccg tgcatcgact tttcttcagc gcggagctcc gagggcatct
     1681 tgaaataaac cagcgcctgc tgcatgccgc cgtaaataat gtagccaagc gcggcgagta
     1741 tgacggcacc gccgatcaaa aatcgtttag cttttgtcat ggttggcggc cgcctccgac
     1801 gaccttaagc ggttgagttc ggcttcggcg gcgttatagc ggcgcttcaa caaaaaccaa
     1861 tacatgagga tgacgccgca aacaattccg taggcgagcg agacatatcc ccaggcgccc
     1921 atcaaactcc tcgctccaag cgcaaggctt tgatctcctg gcgcatctcg ccgacgcggt
     1981 agcgcagcga caacaaataa aagtacagca ggagaaaacc gacgaaactg atcgtcaggg
     2041 cggtcagcat ggctgggtgg acattctccc acggcgcttt gtcggggcgc agaatcgatg
     2101 gcggctggtg caacgtacgc caccaataga ctgacatgtg aatcagcggg atgtcgagaa
     2161 aaccgagaat cccgaggatc gccgccgccg aggcagcgcg ctgttcgtct tcgattgacg
     2221 agcgcagcat caaatagccg gcatagatga gtagtaagat cgccgtcaaa gtgagccggg
     2281 catcccaagt ccaccaaact ccccaagtcg gcttgcccca gatggaacct tctatgattg
     2341 tcagagcggt gaagagtacg cccaattcgg cggcggcgtg ggcgaggagg tcgtccttgt
     2401 tgtcgcgctt ccaaagatac atgccactgc caatgaagac taaaaagtag gagagataag
     2461 cggtcagaat gctcggcagg tgaaggtaca taatgcgctg cacttcaccc tggtaggcgt
     2521 cgggcggcgc caccagcaaa ctccagcaga ggccggccac gacggcgacc aaggccaaga
     2581 ccccgagcat gcctttgcgt cgctttaccg tcttcgtcga ccgctccatg attagctttc
     2641 aatcacccaa tcgaaaatca gcacgccaat ggttaaaaag attacgtcga agccgatgag
     2701 cagccgcagc catgcgatgg catcgccaaa gctaccggtc ctgaggacca tttccatgca
     2761 gcggatcgtt gcgaggatta ccggaatcat taagggaaac aacaccagcg gtaagagcaa
     2821 ctcacgcgct ctggctcgca aggtgattgc cgccgtgatc gtccccagca cacaaaagcc
     2881 caaggtgccc agtaaagtta ccaaaaagag ttgtggtagc aagttccacg agcccaaatt
     2941 gaacaagatc caaaacaatg gaatgacgac cgactcgacc agcagcataa gaattaaatt
     3001 gaatgcggat ttggccaaga atagcgcgcc gcgatcgagc ggcgacagca gcaaggcatc
     3061 cagacaatga ttgtcgcgtt cggcttggaa tagctgcgcc aaacccaaag tgccggtgaa
     3121 gagaaaagcc aaccagagga gcgccggcgc cagttcgacg actttgtccg ggggcatatc
     3181 gaaggcgaag tgaaagacga acagcagcag cgtgccgaaa aagaacatgg tcagcaggct
     3241 gtcgcgccgg cgccactcca atagcagatc cttgcgcaag agcagatagg cttgcctgag
     3301 aagcgtcatg ggccgctgcg cggtaagaaa tcgccgaagg catgctcgaa tgccggccag
     3361 ggtgtttgct ttctttgatc gaactggagc cgcccttgat gcaagatcag cgcgcggtcg
     3421 cacagtttta gtgcgcgcgc gacatggtgc gaggccatga ttacaatgtg tcctcgggcg
     3481 caatgcgctt tcagaaagtt ttccaataag tcgatgccgc tgccatcgag tacgccgtaa
     3541 ggttcgtcga gtagcaacaa cccgggatcg agtagctgcc atttggcaat ggagagccgg
     3601 catttcatgc cggcggacaa gttgccaaca taatcccggg cgcgcgtact gagaccaact
     3661 tgtaataagg ccgagtccag cgcgctggct gaactgtcct tatcgtaaag tgccgtgaaa
     3721 aaacgcaggt tctccacagc cgtcaattga tcgtagagat gctcggcggg cgcgagcaag
     3781 ccgatctggc cgcgctccgc gatggcggtg ccggtgagcg gtcggccgtc gagctcgatc
     3841 acaccggccg tgggcggcgt caaaccggcg agcagtttga gcaacgtggt cttgccagcg
     3901 ccgttggggc cgagcagggc gacgaactcg ccgggcgaga gcgtcaaatc aaaatggcgc
     3961 agtgcccaga ggtaaccata ggctttggcg agttgtttga cggtgaggcg caagattggt
     4021 ccgttgacag cccgcgcgga tcacttcgca gtgcgagcgg ctcgactata gtgatggacg
     4081 gttggggatt caagcagcac gtttaatatc gattcgttat gtcatttggt tgacttttcg
     4141 cagccgagac ctctatgtaa agaggcaatt cagttttcgc gaaatagagg aggaagcaat
     4201 gaatcccgtg gttacggtca ccgggttttg ggacgcattt acaactaaga tcgccgtttt
     4261 tctgccgaat ctgctgtttg ctatcgcgat cattttgttg ggttgggtga tttgcaacgt
     4321 catcaaacgg gtcgtcgtgc gtgtactaaa gatctgccag ttcgacgttc ttgccgagcg
     4381 ctccggcatc aagcagatcc tcgaacgcgg cggcgtgcgc cagactgctt cggatatcgt
     4441 cggtctgttg gcgttctggt ttcttttcct gattgccatc gttaccacgc tggagacgct
     4501 taacctgagc ggcgcaacgg atacgttgca tacgatctat ctttacattc caaagatcgt
     4561 cgcagcgttg gtgacgctca tcttagggct ctactttgct aattttctcg agtcggttac
     4621 ccggacttcg tgcgccaacg ctggattgga ggccgcgcca tcgctcggcc gcgcggcgta
     4681 tatcggtacc agcattttcg tcatcgccgg tatattcgaa attctcgata tcgcgtcgga
     4741 gatcgtcatt tgggcgttca ttttagtatt cggcgcagtc tgtttagccc ttgccttggc
     4801 gttcgggttg ggcggccgag atgtcgccgg ccgctacctc gaaaggtggt tagagcagaa
     4861 gaaaaacgag tagttagcta ccttgactac gagtacccaa tcagttagtt tcaagcagtt
     4921 aaaaaattat tttcctgcaa atagcctaaa gcgcgcgcaa ttgttcgtcg ctcttaggct
     4981 ttttttgcac gtgaggagat
//
LOCUS       DATLCH010000308         4721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7187001, whole genome shotgun sequence.
ACCESSION   DATLCH010000308 DATLCH010000000
VERSION     DATLCH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7187001"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VMZ02_09090"
     CDS             complement(<1..1352)
                     /locus_tag="VMZ02_09090"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR72130.1"
                     /translation="MAQDMRTWIDQLEKAGLLARITKPVDPRTQMGALLWQARDRGLL
                     FENLPGYPGWRCLGQAPGDVALAPVAFGKPRDEMIPEFVRRTETLGKTRLVETGPVQQ
                     RVLLGNDVDITKMPIHQAGIRDGGPFIGSGLMITKNPDTGQRNLSFHRLQMKGPNKTG
                     ILLYPRHAWTNYQMYEAQDKPMPVAIMIGHHPMYYFAAATTTQYGVDEFEIASALLQE
                     DVELVKCRTVDLEVPAWAEIVLEGEIPPKIREPEGPFSEFQDYYLTGSGQNPIVNINA
                     ITMRNDAIFKNVQNGTEVEGCVYHKVPMSAQILRRARNVGGLADVRNVLVMPGIFGVV
                     VQMKPRYYGEAKNVLMSVLSSEYQHPKIAIAVDIDVDIFNYAEVLWSINTRVNPSEDI
                     VTIPGAKIHAMDPSCPEFGHPGAPGWHRIGGKMIIDATKPPECDARRRSEFERLKPVG
                     "
     gene            1621..2847
                     /locus_tag="VMZ02_09095"
     CDS             1621..2847
                     /locus_tag="VMZ02_09095"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72131.1"
                     /translation="MHSIAGFFGLEKLAPEKTRALSIVYAASLALMMGVNFIQPALPA
                     LTQPFQVSDAQLSWMMTLFTAPAIFLSPIFGVIADLYGRRLLLAYGLMVFGVFGAAMA
                     FAPNYTWLLIFRTLQGIGFAAVIPLTIVLIGDLLEGDKEISGQGLKVFLDRIGYFIFP
                     PLGGLLATIAWFWPFVFYILAVPLGLIALFCMPETQGKHKPRATNYLGDILRLSRHPR
                     LVIAFTAGFLRFFLDYGFLTYFPLFLVRTHGISTATAGLLYIFFAIGAMMTSSQAGRI
                     AAGRDKANILFLAFAVSGASVLAVPFIPGVWLIGGALFFYGLANGVISPMQKSLLTQN
                     APAELRGGIVSFDRLIQQVSKTTSTTIVGLLLVSAELPTIFWMLGILSFISVALMALL
                     LPRSAKILPKPTYTSS"
     gene            complement(2911..4014)
                     /locus_tag="VMZ02_09100"
     CDS             complement(2911..4014)
                     /locus_tag="VMZ02_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR72132.1"
                     /translation="MFHGLWAILKKEVIHIKRDPATRFVFAIPLLELLLFGYAIDTDV
                     KHVPTVVFDLNRQQESRQLVQEFEGTQYFRLVGEVHSDQELQKAIVSGRAKVGIKIPP
                     DYSTNLMNGRQGQVQVIIDGSDSTIATRVLNASQQLGFIKSLERDGLSPSQFSIDVRP
                     RLLFNPNLLSVNFYVPGLIGIILQLVTVFLTAFAIVRERERGTLEQLLVTPVSKGGLI
                     VGKLLPYTAIGFGQTVLVLLVMVFLFGVPIHGSAALLLFLSMVFLVPALGMGIVISTF
                     ARNQSEAMQMSLMTLLPAFLLSGFVFPRESMPLLIYLISFLIPATYYLEILRGIILRG
                     AGIAALWDEGLVLAAFGGFFMFLSVRRFKKHLG"
     gene            complement(4014..>4721)
                     /locus_tag="VMZ02_09105"
     CDS             complement(4014..>4721)
                     /locus_tag="VMZ02_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR72133.1"
                     /translation="SQRFSLYEDLTVAENIEFYGAIYGLSGTALAARRRAVIDLVGLD
                     DRLEQMAAILSGGYKQRLALACALLHEPKIVFLDEPTAGIDPVARREIWDLLFRLAAE
                     GTTLFVTTHYMDEAERCSQVAYIYQSKLIAIGRPQDLKRLPEITPPGCTWVEVTSEDP
                     TQLLPLLRGQGDVVDATIFGEAIHALVRQPTGPAALIDFARQHGALDVACRAVPPSLE
                     DVFVSLTRLRRSATEGG"
BASE COUNT         1009 a         1390 c         1265 g         1057 t
ORIGIN      
        1 caacctaccg gtttgagtct ttcaaactcg ctgcgccgac gggcatcgca ttccggcggt
       61 ttggtcgcgt cgatgatcat tttgccgccg atccgatgcc aacccggcgc gccagggtgg
      121 ccgaattctg ggcaagacgg gtccatggcg tggattttcg cgccgggaat cgtaacgatg
      181 tcctctgacg gattaactcg cgtgttgatc gaccacagca cctccgcgta gttgaaaata
      241 tcgacatcga tatcgacggc aatggcgatc ttgggatgct ggtactcgct cgagagcacc
      301 gacatcaaga cgtttttcgc ctcgccgtag tagcgcggtt tcatttgcac cacgacgccg
      361 aaaatccccg gcatcactaa aacattgcgc acgtctgcca gcccgccgac gttgcgcgcg
      421 cgccgcagga tctgcgccga catgggcact ttatggtaga cgcaaccctc cacttccgtg
      481 ccgttctgaa cgtttttgaa gattgcgtcg ttgcgcatgg tgatagcgtt gatattgacg
      541 atgggatttt gtccggaacc ggtcagatag tagtcttgga attcactgaa cggaccctcg
      601 ggttcgcgga ttttcggcgg gatctcgcct tcgagcacta tttccgccca ggcgggcact
      661 tcgagatcga cagtgcggca cttgaccagc tcgacgtctt cctgcagcaa cgccgaagcg
      721 atctcgaatt cgtcgacgcc atactgcgtc gttgtcgcgg cggcaaaata atacatcgga
      781 tgatgaccga tcatgatcgc caccggcatc ggcttatcct gcgcttcgta catttgataa
      841 ttggtccagg cgtggcgcgg atagagcaaa attccggtct tgttcggccc cttcatttgc
      901 aagcgatgaa agctcaggtt gcgctggccg gtgtcgggat tcttggtaat catcaaaccc
      961 gagccgatga acggtccgcc gtcgcggatg ccggcttgat gaatcggcat cttggtaata
     1021 tcgacatcgt tacccagtaa aaccctctgc tgcacggggc cggtctcgac caagcgagtt
     1081 ttgccgagag tttcggtgcg ccggacgaat tcgggaatca tttcatcgcg cggcttgccg
     1141 aaggcgacag gtgccaacgc gacatcgccg ggcgcctggc ctagacaacg ccagccggga
     1201 taccctggaa gattttcaaa gagcaaaccg cgatcgcgcg cttgccagag cagcgcaccc
     1261 atttgcgtgc gcgggtcgac cggcttggta atgcgcgcca acagtccggc cttttccaat
     1321 tgatcgatcc aagtgcgcat atcctgagcc atgagaattc tccgtccacg ttttaaaatt
     1381 tgctaatcca tatcatggag ccgccacgct gaccaatgcg tgaattggag ggttgtggtt
     1441 cagttttaat tgcgcacaga tttccaacct gacaaagatt tctcgttgtc atctcgaacc
     1501 aagtgagaga tctctatcta actgaaacac tacgaattgc agtgatttcc aaccgccaat
     1561 actccattac tcccgtcctt cagtttgcct ttcctgggtg attcgcctac tttccgcgtc
     1621 atgcactcca ttgccggttt ttttgggttg gaaaaacttg cgccggaaaa aacgcgcgca
     1681 ctgagcatcg tctatgcggc gagcttggct ttgatgatgg gcgtgaattt cattcagccc
     1741 gccttgccag cgctcactca gccgtttcag gtcagcgacg cccagctctc gtggatgatg
     1801 acgctgttca ccgcgccggc aatcttcctt tcgccgatct tcggcgtcat cgccgatctc
     1861 tacggccgcc gcttgctgct tgcatacgga ttaatggtat ttggcgtgtt cggcgcagca
     1921 atggcatttg cgcccaatta cacttggctg ctgatttttc gcacgctaca aggcatcggc
     1981 tttgcggccg tgattccgct caccattgtc ttgatcggcg atctgctgga gggcgataag
     2041 gaaatcagtg gccagggtct gaaagtgttt ctcgaccgca tcggttattt catatttcca
     2101 cctctcggcg gactgctcgc gacgatcgca tggttttggc cgtttgtctt ttacatactg
     2161 gctgtcccgc tcggtttgat cgcgttgttt tgcatgcccg agacccaggg caagcacaaa
     2221 ccgcgcgcca ccaactatct aggcgatatc ctgcgcctga gccgccatcc ccggctggtg
     2281 atcgcgttca ccgccggttt cctgcgcttt tttctggact acggatttct cacttacttt
     2341 ccactgtttc tagtgcgcac ccacggaatt tcgaccgcca ctgccggtct gctctacatc
     2401 tttttcgcca tcggcgccat gatgacatcg agtcaggccg ggcgcatcgc cgccggccgc
     2461 gacaaggcga atattctgtt cctcgctttt gcggtcagcg gcgcgtcggt gttggcggtg
     2521 cctttcattc caggcgtttg gctgatcggc ggcgcgttat ttttctacgg ccttgccaac
     2581 ggtgtgatct cgcccatgca gaaaagtttg ctcacgcaaa acgccccggc agaactgcgc
     2641 ggcggtatcg tctccttcga tcgcttgatc cagcaagtgt ctaaaaccac ctcgaccacc
     2701 atcgtcggtc tgctgttggt gagcgcggaa ctgccaacga tcttctggat gctgggtata
     2761 ttgtcgttta tcagtgtcgc cctcatggcc ctgctgctac cgcgcagcgc aaagatcctg
     2821 ccgaagccaa cctatacttc ctcatgatcc gttgccgggt tcttgaaata tgaaatttta
     2881 aatctggaat tccgagcgcc agcgaggtga ctaacccagg tgctttttaa accggcgcac
     2941 gctcaagaac ataaaaaaac caccgaaggc cgccagtacc aatccctcat cccacagtgc
     3001 cgcgatgccg gcgccgcgca gaatgatgcc gcggaggatt tccagataat aggtcgcggg
     3061 aatcagaaaa ctgatcagat agatcagcaa tggcatcgac tcccgtggaa aaacgaagcc
     3121 ggacagcaga aaggccggca acaacgtcat caaggacatc tgcatcgctt cgctctgatt
     3181 gcgcgcgaag gtcgaaatca cgatgcccat gccgagggcg ggaacgagaa agaccattga
     3241 caaaaacagc agcaaggcag ccgaaccgtg aatcggcacg ccgaacagga aaaccattac
     3301 caataacact agcaccgtct ggccgaagcc gatggcggtg tagggtaaaa gcttgccgac
     3361 gatcagtcct cccttggata ccggcgtgac cagtaactgt tccagcgtgc cgcgctcgcg
     3421 ctcgcgcacg atggcgaagg ctgtgagaaa aacggtgacc agttgaagaa tgatgccgat
     3481 caatcccggc acgtagaagt tgacgctcaa caaattggga ttgaacagca agcgcggccg
     3541 gacatcgatg ctaaattggc tcggcgacag gccatcgcgt tccaaagatt tgataaaacc
     3601 caactgttgg ctggcgttga gcacccgggt ggcaatggtc gagtcggaac cgtcgatgat
     3661 aacttgcacc tgtccttgcc ggccgttcat caggttagtg gaataatccg gcgggatttt
     3721 gatacccact ttagcccgtc cgctgacgat agctttttga agctcttggt ccgaatgcac
     3781 ttcgcccaca agtctaaaat actgcgtgcc ctcgaactcc tggaccaatt gtcggctctc
     3841 ctgttggcga ttgagatcga acacgacggt gggcacatgc ttgacgtctg tatcgatggc
     3901 gtaaccgaac aacagcagct ccaacagcgg tatggcgaag acaaagcgcg tcgccggatc
     3961 gcgcttgata tggataacct cttttttgag gatcgcccat agaccgtgaa acattagccg
     4021 ccttccgtcg ccgaacggcg caaccgcgtc aacgaaacga agacgtcctc gagcgacggc
     4081 ggaactgccc ggcaggcgac gtcgagcgcg ccgtgctggc gggcaaagtc gatcaaggcc
     4141 gccggcccgg taggctggcg cacgagagca tgaatcgcct cgccgaagat ggtcgcgtcg
     4201 acgacgtcac cttgacctct gagcagcggc aaaagctgcg tcggatcttc acttgtaacc
     4261 tcgacccacg tgcaacccgg cggcgtaatt tccggcagcc gtttcaaatc ctgcgggcgg
     4321 ccaatggcga tcaatttgga ttgatagata tacgccacct gcgagcagcg ctcagcctca
     4381 tccatgtagt gcgtcgttac gaacagcgtc gtgccttcag cagcgagacg aaacagcaag
     4441 tcccaaatct cccgccgcgc taccggatcg attcctgccg tgggctcgtc gagaaaaacg
     4501 atcttgggct catgcaacaa cgcacacgcc agcgccaaac gctgcttgta accgcccgat
     4561 aggatcgccg ccatctgctc cagtcggtca tccaatccga ccagatcaat caccgcacgc
     4621 cgccgcgccg ccagtgctgt cccgctcaaa ccgtaaatgg cgccgtaaaa ttcgatattc
     4681 tccgccaccg tcaaatcctc gtagagacta aaccgctgcg a
//
LOCUS       DATLCH010000309         3438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7188416, whole genome shotgun sequence.
ACCESSION   DATLCH010000309 DATLCH010000000
VERSION     DATLCH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7188416"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1582
                     /locus_tag="VMZ02_09110"
     CDS             47..1582
                     /locus_tag="VMZ02_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72134.1"
                     /translation="MKRVALEFNRRNFLKLGAFAGAGALADSLWPNTSWSASRERLTI
                     LSSVALDNLHPYAYSSGPQYGIWYNMIEPLVDVDFAKRSYFGVLAESWEFHGKKWIFK
                     LRKNVRFHDGSRFTAADVIYSFNRVKNDKQSLQKENFRDLVEMQALDDHTIAFVTEAP
                     NAVFLDRLENRFIIAKSATEAQGGEPGEQKPVGTGPYRFVSWQREGNLVLNRHENYWG
                     GKAAVKEIVIRRVREDAGRVAGLLAGQGDVINNVPVEELSRFDKHAKVKAETVEGQRM
                     YFLAMNVTHKPFDNKLVRQAINHAVDPTTIIKHIYEGNGYVMNGPMGSKVIGFDPKIK
                     RYPVDLKKAKELLDKAGFPNGLEVKLYFSPDRYPKAKEVCQVIADQLAKIGIKVELVS
                     QEFAVFWGKEWVNGGKTPFYYVGRPATDADTVYDQYYRSGVSPRIQYKNPEFDKIIDE
                     EQRTGDAKKRVALLHQAGRILMEDVPFVPLYTLAEIYGTARNVAWNARPDEKILAGTM
                     KII"
     gene            1789..2334
                     /locus_tag="VMZ02_09115"
     CDS             1789..2334
                     /locus_tag="VMZ02_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72135.1"
                     /translation="MATGLLNAVPPAQTKMRYASTLRCFGQFLEGMELKALEVKTHND
                     DYILQAWNKGTSMNLDLEKHFTPEEVMKLEAEGRANRKPFTGPPNMLSLAQVLRLSGN
                     YVDRMHGRLVRVSWQDQSDKIQSITVQWEASPAGREPVESQLAVIEELCIHIYKQRKK
                     INVASERQAHRPFVSVAKVTS"
     gene            complement(2405..>3438)
                     /locus_tag="VMZ02_09120"
     CDS             complement(2405..>3438)
                     /locus_tag="VMZ02_09120"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72136.1"
                     /translation="VKKVAPEFNYLQLKDGIVFALDQCLQRGVTTVHDIVRYAEPVRA
                     YQELYKEGRMNARVSILPRVIESMIETKSLTELGLITGFGNEWLRIGGVKMSIDGGIT
                     GRNACFHEPYENDEHNHGIIRIQQDELNHTVQVCHDAGLRCCVHAIGDKAFDMALDAY
                     ENAIEHSPRKDHRHRIEHMGNWLATPKLIGRMVRSGIVAVPNIAFAYYIGDAILDCVG
                     ERRLTKAFPFHTLLKNGVIMSGGSDSPGYWPVDPLRDIATAASRKMRWGEVWIPEEKI
                     TVGQAFAMHTTTASWVGFEENEKGTLEVGKLADIAVLAEDPFALEPDKIRELKVEMTL
                     VGGEVMYQA"
BASE COUNT          811 a          906 c          954 g          767 t
ORIGIN      
        1 cgcggagatg aagattcgtt aacgccggga agaaggagaa tccgccatga aacgagttgc
       61 actcgagttc aatcgccgca attttctcaa gctcggcgcg ttcgccggcg ctggcgcctt
      121 ggccgactct ttgtggccca acaccagttg gtcggcatcg agggagcgct tgacgattct
      181 tagcagcgtc gctctcgata acttgcatcc ctacgcttac agctcaggtc cgcagtacgg
      241 catttggtac aatatgatcg agccgcttgt cgatgtcgat ttcgccaagc gcagctactt
      301 tggcgtgctc gccgagtcat gggagtttca cggcaaaaaa tggatcttca agctacgtaa
      361 gaacgtgcgt tttcacgacg gcagccgatt taccgcagcg gatgtgattt attcattcaa
      421 ccgagtaaag aacgacaagc agagcctgca gaaagaaaac tttcgcgatc tggtcgagat
      481 gcaagcgctc gacgaccata ccatcgcctt cgttaccgag gcgcccaatg ccgtgtttct
      541 cgaccgcttg gagaatcgct ttatcatcgc caaaagcgcc accgaagcgc agggcggcga
      601 gcccggagag cagaagccgg tgggcacggg tccgtaccgg ttcgtcagtt ggcaacggga
      661 aggcaatttg gtcttaaacc gtcatgaaaa ttactggggc gggaaggcgg ccgtcaaaga
      721 aatcgtgatc cggcgggtgc gcgaagacgc gggacgagtc gccggcttac tggccggtca
      781 aggcgatgtg atcaataacg tgcctgtgga agagctgtcg cgtttcgata agcatgccaa
      841 ggtgaaagcg gaaacggtcg aaggccagcg gatgtatttt ctcgccatga acgtcaccca
      901 caaacccttc gacaataaat tggtccgcca agcgatcaat catgccgtcg atccgacgac
      961 gatcatcaaa cacatttacg aaggcaacgg ctacgtgatg aacgggccga tgggctcaaa
     1021 ggtgattggc ttcgatccca agatcaaacg ctacccggtg gatttaaaaa aagccaaaga
     1081 gcttctcgac aaggcgggat ttcccaatgg cttggaagtg aagctctatt tttcaccgga
     1141 tcgttatccg aaagcaaagg aagtctgcca ggtcattgcc gatcagctcg ccaagatcgg
     1201 tatcaaggtt gagctggtat ctcaggagtt cgcggtgttt tggggcaaag aatgggtcaa
     1261 cggcgggaag acgccctttt actacgttgg ccggccggcc accgacgccg atacagtcta
     1321 tgatcaatac tatcgctccg gcgtcagtcc gcggattcag tataaaaatc ccgagttcga
     1381 taagattatc gatgaagaac agcgcaccgg cgatgctaag aagcgcgtcg ccttgctcca
     1441 tcaagccggg cgcattttaa tggaagacgt gccattcgtg ccgctctata cacttgcgga
     1501 gatctacggg acggcgcgca atgtcgcttg gaacgctagg ccggacgaga agatcctcgc
     1561 cggaacgatg aagatcattt aagcgcttca gttctcgagg tttcacttca gtgatgtaag
     1621 acatttagta acgttgagat ttcaattgtc ggttttctaa aaattctgcc cgccgacgag
     1681 aatgtttgcc gtgcgcacaa aattgcacgg caacaacaac gattcataga aaattataga
     1741 aaccttcgca ctattccttg gcatgcccgt tgctgcttat ttgtccttat ggcaacaggt
     1801 cttcttaatg cggtaccgcc ggcgcaaacc aagatgcgct atgcctcgac tctacgctgc
     1861 tttggccaat ttctcgaagg catggagctc aaggcgttgg aagtcaaaac tcacaatgac
     1921 gactatatcc tgcaagcatg gaacaagggc acatccatga acttggattt ggaaaagcat
     1981 ttcacgcccg aagaggtgat gaaactcgaa gccgaggggc gcgctaatcg caagccgttt
     2041 accgggccgc ccaatatgtt gagcctggcg caagttttac ggctttctgg taactacgtc
     2101 gatcgcatgc atggacggct ggtgcgggtg tcttggcaag atcagtcgga caaaattcaa
     2161 tcaatcacgg tgcagtggga agcgagcccg gcaggtcgcg agccggtgga gtcacaactc
     2221 gctgtcatcg aagagctgtg cattcacatc tataagcagc gcaagaaaat caacgtggcg
     2281 tccgaacgac aagcgcaccg gccgtttgtc agcgtcgcca aggtcactag ttagacgatc
     2341 ccgagcaggt ttgaggaaat aaaaaaggcg gccgcgtggc cgcctttttt tatttccaag
     2401 acagttaagc ttgatacatc acttcaccac cgaccaacgt catctcgacc ttgagctctc
     2461 tgattttatc gggctccagc gcaaatgggt cctcggccag aacggcgata tccgcgagct
     2521 tgccgacttc gagcgtgcct ttttcatttt cttcgaagcc aacccaggat gccgtcgtcg
     2581 tgtgcatggc aaaggcttgc cccacggtaa ttttttcctc ggggatccag acttcgcccc
     2641 agcgcatttt gcgcgatgcg gcagtggcga tatcgcgcag cgggtcgacc ggccagtagc
     2701 ccggcgaatc ggaaccaccg gacatgatca cgccgttttt caatagagta tgaaatggaa
     2761 atgccttggt cagccgccgt tcaccgacgc aatcgagaat cgcatcgccg atgtagtaag
     2821 caaaggcgat attcggcaca gctacgatac cggagcgcac catgcggcca atcaacttcg
     2881 gcgtcgccag ccaattgccc atgtgctcga tgcggtggcg atgatcctta cgcggcgaat
     2941 gctcgatggc gttttcatag gcgtccagcg ccatatcgaa ggctttgtcg ccgatggcat
     3001 gaacacagca gcgcaagccg gcgtcgtggc atacctgcac ggtgtggttc aattcgtctt
     3061 gttggattcg gatgatgccg tggttatgtt cgtcgttctc atagggctcg tgaaaacagg
     3121 cgttgcgccc ggtgatgccg ccgtcgatgc tcatcttgac gccgccgatg cgcagccact
     3181 cgttgccgaa gccggtgatg aggccgagct cggtcaagct tttggtttcg atcatcgatt
     3241 cgatgacccg cggcaagatg cttacgcgcg cgttcatgcg cccttctttg tagagttcct
     3301 ggtaggcgcg caccggttcg gcgtagcgga cgatatcgtg aaccgtggtt acgccgcgtt
     3361 ggagacattg atccaatgcg aagacgattc catctttcaa ttgcaggtaa ttaaattccg
     3421 gggcgacttt tttaacaa
//
LOCUS       DATLCH010000310        11890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10916278, whole genome shotgun sequence.
ACCESSION   DATLCH010000310 DATLCH010000000
VERSION     DATLCH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10916278"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VMZ02_09125"
     CDS             complement(<1..637)
                     /locus_tag="VMZ02_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR72137.1"
                     /translation="MKVTVLDDYQHAIAPTKAIARLRQNAEVQILHEKLGSEAALIEA
                     IKACQAIIPIRERTKFTASLFQQLPALEFISQTGNHAYHIDMDAATAHGVVVSLAPGG
                     NSATELTFGLMLDVMRKISRADQAMRHGEWPLILGHVLKGKTLGILGLGKIGTEVAAI
                     GRAFGMKVIAWGPTLTQERAGKSDATFMMLEDVLRSADVVSVHLKLSEDSKN"
     gene            complement(731..1120)
                     /locus_tag="VMZ02_09130"
     CDS             complement(731..1120)
                     /locus_tag="VMZ02_09130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HUR72138.1"
                     /translation="MPRKDIQPKTLSDPRPRYAQAIQTDGGKLLFIAGQTASDKDGKV
                     VGKGNIKKQTEQVYANILAVLEEAGGSIDNLVMTTTYIVDRKYRVGYNEVRMKQYKEN
                     SPTSTLVIVKGLAHPDYLIEIAGIAVI"
     gene            complement(1236..2333)
                     /locus_tag="VMZ02_09135"
     CDS             complement(1236..2333)
                     /locus_tag="VMZ02_09135"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72139.1"
                     /translation="MKWRRDIRVSEKIGVLESCSNRGSGLSRKASIRHSPIPFLLLVC
                     VAASWLIGGTANAQQLKRIKIGYPSLSFRQSNVWVAREMGLFAKYGLEVEPILFRGGQ
                     VATQALVSGDPPIVNIGTVVQATIQGHNLVLTAAVETKYDLIVFSRTGITQLEQLKGK
                     RIGITGFNSATHYASIILARHLGADLKEFTLIPAGLDTERIASVQSGVIDATYLSSSA
                     APLGRRAGLVEHVSIGDLGVEVQGNGFATSRAYIASNRETVRSALRGFVEAIYFVYSN
                     KAATQKVFAKYMRTNDPAVLEDSYNGYIKSIPKKPYPTLKGIQFMLDVLAPTLPNAKN
                     FKPEQFVDLSFLQEMEKDGFFNEMAKRYPVR"
     gene            complement(2302..3309)
                     /locus_tag="VMZ02_09140"
     CDS             complement(2302..3309)
                     /locus_tag="VMZ02_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72140.1"
                     /translation="MKIASLALSLLVNLSIGLAATVEAQTPPLKKIRIGYPSLSFRQS
                     NVWVAREMGLFTKYGLDVEPIFLRGGQMATQALVAGDPPIVNIGTVVQASLQGYNLVL
                     VAAVETKYDQIVFARKGINKLEELKGKKFGISGYGSATHNAANILFQHLNLEINKDIT
                     LVPTGPDAERLGAMLAGRIDATFFTSSASAPARKSGFNELFSVADLGVEVQGNGFATS
                     RGYIASNRDTVKSALKGFVEAIYFVYANKAAAQKVFAKYMRTNDPEMLEDSYNGYIKW
                     IPKKPYPTLKGIQYMLDLLAPQLPQAKNAKPEQFVDLSFLQELEKEGFFNEMAKRYPS
                     K"
     gene            complement(3525..6032)
                     /locus_tag="VMZ02_09145"
     CDS             complement(3525..6032)
                     /locus_tag="VMZ02_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72141.1"
                     /translation="MTTHRTCRLGVAAVIAVLLGAPALCRAQARELDVEKILNPMPEY
                     DPFDTAPAAPQFFPDAVDKRSRDLLIDTLTNRNETLKDHLEFLKREDARLQKEQRTTT
                     GLTERAQDLVNNTIQDREKNLAAYRQALREASTMERRKYLEAVINRDDHFQSEQLMRQ
                     SSTNFWGGMVNRLLSSIDLVGVASGNYVGAAAETAISQLYALMDRDMSMEERRALARD
                     LDHLKRYPDDPKNAQVRKQIASLDKKKRDVLVAKQLAKAKEALNKNDDERALFHAQVA
                     LFFDPQSQNAEKVRLQTAQVLQQRQETRQQSLKARSEANTTPQQQTDVRKLLEALSLR
                     DANLIQRVAIDTEKKYLGKPLADAARDAEAVALEIKGWHEAARKAVEQNARKATTPVT
                     RERADVLLQSPGYNLLSRFNDARTERQLESAKYVLLGDDFLRKNMLFAAGAMAVAGPG
                     GAATLGMANAMMWSRQLFQVMTNNPISAQPVIDAGVAYVRGHPKSENAGDVYRVLAEA
                     YEERGLVERALTYHELAGSPKEKIAALNDKAARALLAAASKSPSRSSQENYLKAVIDY
                     HPDSSAAAEATKKLAALAKDENQGMRMSKQFLLEHSEIWGPRGLGLKRTLFDGDPRNM
                     EIADRGAILLSDSELLIFYQTPWGVRSQSHRLAKANAERFFVALREKNQQVAAADANQ
                     RGKDSVGGIRNVPTAIIRGEREQRSTNSEERDDTTFRLIREAGGSSYRRVLDAQLITE
                     NERNPGSQYQLPPIQGSISASHFAMSGGLPAGFGGSQLSVGADRGTPYGGVQIPIPLL
                     QGFIPVDFMVQGRPGGVSVFPRIRTGVDRGSDPELYR"
     gene            complement(6029..6892)
                     /locus_tag="VMZ02_09150"
     CDS             complement(6029..6892)
                     /locus_tag="VMZ02_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72142.1"
                     /translation="MPFVHRARAAVPEGRYLPAGDLLDIVRDFQRLAKEDTYRFAIPK
                     DVTGINIMKATLTRLQDYETKNRGQFTDIVQFNRALALERLREYDQAAALYRKIAETE
                     GALGSEAAKKAEILDNFLRIFDRSIPLDDPFKYIAGLDEKVAAWNGLILKHRGTPYEF
                     LARVEEERIDRAKVAFVEANRFRLKEGNQLTIVGYSQLITKHQQSKNYQRHLLDFADF
                     YMILAKDYAAQYDPEGLAFDLNVLEQFAKSALKFYSEVAQTDGVIEKLEAQAKIEATR
                     GYMEKLTRLNR"
     gene            7108..8301
                     /locus_tag="VMZ02_09155"
     CDS             7108..8301
                     /locus_tag="VMZ02_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HUR72143.1"
                     /translation="MASTTRQTLLAFIIFVIHLTGCAEGLFEPARPVNPPGPLALPQS
                     PPLPEYRGPATRSFFMGFTLWPADLSRDGVAIAEDYAYAHGDIVAVTFISGIPWAEAL
                     DNKPYPRDVQEHLNYRPPAGKKLFLSISPLDKDRRGLAPYWGDTDTQPLPQPWNREPL
                     NSARVKRAYLNFILRAVETMRPDFLAIGVESNVLLSRNPQKWQQLKELHRDTYLAVKK
                     LHKALPVFFTTDVLHYKRLVADSKGSEQEKEVMDLMRYSDLFAMSVYPHMSLEVARPV
                     PANFLDFATRFKRPVAVAESGMASADVELRTYSTLLRGSDADQYQFTELLLKTAARDY
                     YEFVINFASTDSDRLVARLQPPLDDLARLWAYTGMQTSDKRAKPASAVWDAYRRAKYE
                     QAKWW"
     gene            complement(8383..8715)
                     /locus_tag="VMZ02_09160"
     CDS             complement(8383..8715)
                     /locus_tag="VMZ02_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR72144.1"
                     /translation="MKIITINSALKDLKKISDEIRYADCFKGETFTSGLIAFRPSKNS
                     DAKQIIHDDKDVVCQVIKGSGRLRVNGKRTPLRPGIICHVPKGTPHDFAAGKYGELVL
                     FYSLIVTA"
     gene            complement(8818..9771)
                     /locus_tag="VMZ02_09165"
     CDS             complement(8818..9771)
                     /locus_tag="VMZ02_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72145.1"
                     /translation="MRLVTRADFDGLVCGALITKFENIDDYLYVEPKFMQDGLVEIRS
                     GDIITNLPYHPHCTLWFDHHVTNTTPTFQKPIILGRGGFRLAPSAARVVYEYYEEAAE
                     RKQATGNGSTPEQITEFLGTARMQHLMHEVDRVDAGQLERDDVLNPQGYVLISMTTDG
                     RNAGDEPYWLKMINLLRDATLEEVLNDADVKRRCEQIQREQEKLRKLLLERTCYKGNV
                     IYCDLRGVKEIPDGNRFLVFTLFPKGNIQVKVAYDSQRADTTSISVGYNIFNPTSNVN
                     VGELLKNYGGGGHKVVGSSRVPNDKAEQAIQEILAAVTEVG"
     gene            complement(9768..10883)
                     /gene="ald"
                     /locus_tag="VMZ02_09170"
     CDS             complement(9768..10883)
                     /gene="ald"
                     /locus_tag="VMZ02_09170"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HUR72146.1"
                     /translation="MIIGVPKEIKTEENRVAVTPTGVAGFVARNHQVLIQRGAGSGSG
                     LTDSGYQAAGATIVDSAKQVWDKADMIMKVKEPQESEFAFLRPGLILFTYLHLAADEA
                     VTRAMLERKVTGIAYETIQLDDGSLPLLAPMSEIAGRLSIQVGAWCLQAENGGRGILL
                     GGASGVRPANVVILGAGMSGTAACQVAVGMGAHVSILDINPNKLRYVHDILGGHVTTL
                     MSNRANVEEAVVGADLVIGSVLIPGAQAPKLIPRALVKRMKAGAAFVDIAIDQGGCAE
                     TSRPTTHTNPIYVEEELVHYCVTNMPAIVPNTSTYALTNSTLTYGLELANRGFPQALV
                     RNKALAKGLNTFNGKVTYEGVASAFNLPFTPVDEVIR"
     gene            complement(10973..11338)
                     /locus_tag="VMZ02_09175"
     CDS             complement(10973..11338)
                     /locus_tag="VMZ02_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HUR72147.1"
                     /translation="MERFFFTAGAIAAFIGVALGAFGAHSLKTKISPDMLNIFETGVR
                     YQMYHALSLLGVAWAITRWPDINFTTAGWCFIAGIAIFSGSLYLLSFTGARWLGAITP
                     IGGLAFLGGWAILIWSLNR"
     gene            complement(11384..>11890)
                     /locus_tag="VMZ02_09180"
     CDS             complement(11384..>11890)
                     /locus_tag="VMZ02_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72148.1"
                     /translation="AETMQVRAELGYPIMVTPLSQFVGSQAAINVIVGERYKEVTDQI
                     IQYALGYWGKEGAELMAPEVKAKILDRPRAKEWAERPPPEPSVAELRKKMNAEHVSDE
                     EFLLRWNLEEKEIETMKAAGAPKEYLTANQPLVKLIDALSQRKDYRQIVVQKGQVIVS
                     FNRSAPAL"
BASE COUNT         2422 a         3247 c         3403 g         2818 t
ORIGIN      
        1 ggttcttact gtcctcggat aacttcaggt gcacgctgac gacatcggcg ctgcgcagga
       61 catcctcgag catcatgaag gtagcgtcgg atttgccggc ccgttcttgg gtcaaggtcg
      121 ggccccaggc gatcactttc atgccgaaag cgcggccgat ggcggccact tccgtgccga
      181 tcttgccgag ccccaaaatg cccagcgttt tgcctttcaa gacatgaccc aaaatcaaag
      241 gccactcgcc gtggcgcatc gcctggtcag cgcgcgaaat ttttcgcatg acatcgagca
      301 tcaagccgaa ggtcagctcc gtggcgctgt tcccgccggg cgcgagactg acgaccacgc
      361 catgagccgt cgctgcatcc atgtcgatgt ggtaggcgtg attgccggtt tgtgagataa
      421 actccaacgc cggcagttgc tgaaatagcg acgcggtgaa ttttgtccgc tcacgaatcg
      481 gaataatcgc ttggcaggct ttgatcgctt cgattaacgc cgcttcgctg ccaagcttct
      541 catggagaat ctgcacctcg gcattctggc gcagccgggc aatggctttt gtcggggcaa
      601 tagcgtgttg gtagtcgtcg aggacagtga ctttcatttg gctgtctcgc cgcagaggcg
      661 cagagaacga ggagaaaaat taaaatttat ctcctcgaac tgtgcgcctc tgcggaaatt
      721 tttatgtctt tcaaatcacc gcgatgcccg cgatctctat aagataatcg gggtgggcga
      781 ggcctttgac gatgaccagc gtgctggtcg gggagttttc cttgtactgt ttcatccgca
      841 cttcgttgta cccgacgcgg tatttgcgat cgacgatgta cgtggtggtc atcaccagat
      901 tgtctatgct gccgccggct tcttcgagta ccgcgaggat gttggcatac acctgttcgg
      961 tttgcttttt aatattgcct ttgccgacca ctttgccatc tttgtcgctc gcagtttggc
     1021 cggcgatgaa cagcaactta ccgccgtcgg tttggatcgc ctgtgcgtaa cgcggccgcg
     1081 gatcgctgag agttttgggt tggatatctt tgcgtggcat gggagctccc ttcgtatttc
     1141 aggttggagt aatggaataa tggagtactg gagtgttggt tttcccgatt tcgcttaact
     1201 gcaccactgc aatactccag tcgtcccgct gttgtctacc tcaccgggta ccgcttggcc
     1261 atttcgttga aaaaaccatc cttttccatt tcctgcaaga agcttaaatc gacgaattgt
     1321 tccggtttga aattcttggc attgggcaaa gtcggcgcca gcacgtcgag catgaattga
     1381 atgcccttca aagttggata aggttttttc gggatcgact tgatatagcc attgtaggaa
     1441 tcttccagca cggccggatc gttggtgcgc atgtatttgg caaatacttt ttgcgtcgcc
     1501 gctttattgc tgtagacgaa ataaatcgcc tcgacgaagc cgcggagcgc gcttctcacg
     1561 gtctcgcgat tactggcgat atacgcgcgc gatgtcgcga agccattgcc ttgaacctcg
     1621 acgcccaagt cgccgatgga gacatgttcg accagaccgg cgcgacgccc cagcggcgcc
     1681 gccgaggagg acaggtacgt cgcatcgatt acacccgact gcaccgaggc gatgcgctcg
     1741 gtgtcgagac cggcggggat cagcgtgaat tccttaagat cggcgcccaa gtggcgggcg
     1801 aggatgatcg atgcgtaatg agttgccgaa ttgaatccgg tgatgccgat gcgcttgcct
     1861 ttgagttgct cgagctgggt gattcccgtc cggctaaaga caatcaggtc gtacttggtt
     1921 tcgaccgcgg cggtgagcac gaggttgtga ccctgaatcg tcgcttggac gaccgtgccg
     1981 atattgacaa tcggcggatc gccgctgacc agcgcctggg tggcaacctg gccgccgcga
     2041 aacaaaatcg gctcgacttc caaaccgtat ttggcgaata aacccatttc gcgcgcgacc
     2101 cagacattgc tctgacgaaa gctgagcgat gggtagccga tcttaatgcg cttgagctgt
     2161 tgagcgttgg cagtgccgcc gatgagccaa ctggcggcga cgcaaactaa caggagaaat
     2221 ggaatagggg agtggcggat tgatgccttc ctagataaac ccgaccctcg attactgcaa
     2281 gactccagca ctccgatctt ctcacttact cggatatctc ttcgccattt cattgaagaa
     2341 gccttccttt tcgagttctt gtaagaagct caagtcgaca aactgctcgg gtttggcgtt
     2401 cttcgcctgg ggcagttgcg gcgcgaggag gtcgagcatg tactggatgc ccttgagggt
     2461 cgggtaaggc tttttgggaa tccacttgat gtaaccgttg taggaatcct cgagcatctc
     2521 gggatcgttg gtgcgcatgt atttggcgaa cactttctgc gcggcggctt tgttggcgta
     2581 gacaaaatag atcgcttcga caaaaccctt tagagcactt ttcacggtgt cgcggttgct
     2641 ggcaatataa ccgcgcgacg tggcgaatcc gttaccctgg acttcaaccc ccaaatcggc
     2701 aacggagaat agctcattga agccactctt gcgcgccggc gctgacgctg aagaggtaaa
     2761 gaatgtcgca tcgatccttc ccgccagcat ggcgcccaaa cgttccgcgt ccggccccgt
     2821 aggaaccagc gtgatgtcct tgttaatttc caagttgaga tgctggaaca gaatgttggc
     2881 tgcgttgtgg gtcgccgagc cgtatccgct aatgccgaat tttttgcctt tgagctcttc
     2941 gagtttgttg atgcctttgc gggcaaatac aatttggtcg tacttggttt cgacggcggc
     3001 gaccagtacg agattgtagc cctgcaggct tgcctgcacg accgtgccga tgttaacgat
     3061 gggcggatca ccggccacga gggcttgcgt cgccatctgg ccgccgcgca aaaagatcgg
     3121 ctcgacatcc aaaccgtatt tggtgaacag gcccatttcc cgcgccaccc aaacgttgct
     3181 ctgacggaaa ctcaacgacg gatagccgat gcggattttc tttagcggcg gagtctgtgc
     3241 ctcaaccgtt gccgcgaggc caattgataa gttaaccaga agactgagtg caagtgatgc
     3301 gattttcata ctatttcttc ctgctcatcc tttctctgaa tatgtgtgac ttaacatttc
     3361 aatagcttga aagccgcgaa tttattcctt cattgagtaa taggaggctc gacaaagtca
     3421 ctcattggcc ggattgtccc gccttgttag ctcttattta aagtcttcgc gcgcggcggt
     3481 gcagaccagc taatacaacc cgcggtaatt tggtgagttc cccatcagcg atagagttcc
     3541 gggtccgagc ctcgatccac gccggtgcga atgcggggaa ataccgagac gccgccggga
     3601 cggccttgaa ccatgaagtc gacgggaatg aatccttgca acagcgggat cggaatctgc
     3661 actccgccgt aaggcgtgcc acgatcggca ccgaccgaga gctgactgcc gccgaaaccc
     3721 gcgggcagac cgccgctcat cgcaaagtgg cttgccgata tgctgccttg gatcggcggg
     3781 agttggtact ggctgccagg attacgctcg ttttccgtga tcaactgcgc gtcgagtacc
     3841 cggcgatagg acgagccgcc ggcttctcgg atcaacctaa acgtcgtgtc gtcgcgctcc
     3901 tcagaattgg tgctgcgctg ttcgcgttcg ccgcggatga tcgcggtggg gacatttctg
     3961 attccgccga cgctgtcttt gccgcgctgg ttagcatctg ccgcggcgac ttgctgattt
     4021 ttttcgcgca gggcgacgaa gaaacgctcg gcgttcgcct tagcgagacg atgactctgg
     4081 ctgcgcacgc cccagggagt ctgatagaag ataagtagct cgctgtcgct gagcaaaatg
     4141 gcgccgcggt cggcgatctc catgttgcgc ggatcgccat cgaacagggt gcgcttgagt
     4201 cctaaccccc gcggccccca aatttcgcta tgttccaaca aaaattgctt gctcatgcgc
     4261 atgccttggt tttcatcctt ggcgagggcg gcaagttttt tggtcgcttc ggccgccgcg
     4321 ctcgaatcgg gatggtaatc gatcacagcc ttgagatagt tctcttgact gctgcgcgaa
     4381 ggtgatttac tcgcggcggc caataaagcg cgggcggcct tgtcattgag cgctgcgatt
     4441 ttttcttttg gcgagccggc gagctcatga taggtgaggg cgcgctcaac caatccgcgc
     4501 tcctcgtaag cctcggcgag gacgcggtaa acatcgccag cgttttccga cttgggatga
     4561 ccgcgcacat aggcgactcc cgcatcgatc accggctgcg ccgaaatcgg gttgttggtc
     4621 atgacttgga aaagttggcg gctccacatc attgcgttgg ccatgcccaa agtcgcagcc
     4681 ccccccggac cagcgaccgc catggcgccg gcggcgaaga gcatgttctt gcgcaggaaa
     4741 tcgtcgccga gaaggacgta tttcgccgat tctagttgac gctcggtgcg cgcatcgttg
     4801 aatctcgaga gcagattgta tccggggctc tgcagcaaga catccgcgcg ttctctggtc
     4861 acaggtgtgg tcgccttgcg ggcgttttgc tcgacagcct tgcgtgccgc ttcatgccag
     4921 ccttttattt ccaatgcgac ggcttcggca tcacgagcgg catcggccag tggcttaccg
     4981 aggtattttt tctccgtgtc gatcgccacc cgttggatca agttggcatc gcgcaaggac
     5041 agcgcctcga gcagctttct cacatcggtt tgctgttgcg gggtcgtatt cgcttcgctg
     5101 cgtgctttaa ggctctgctg gcgcgtctcc tgacgctgct gcaacacctg ggcagtttgc
     5161 aggcgtactt tctcggcatt ttgcgactga ggatcgaaga agagcgccac ttgggcatga
     5221 aagagtgctc gctcgtcgtc gttcttgttc aaggcttctt tggccttggc gagttgcttg
     5281 gcgaccaaaa cgtcgcgctt cttcttatcg agcgaggcga tctgcttgcg cacctgagca
     5341 ttcttgggat cgtcgggata gcgttttaga tgatcgaggt cgcgcgctaa cgcgcgccgc
     5401 tcttccatcg acatgtcgcg atccattaaa gcatagagct ggctaattgc agtttccgcg
     5461 gcggcgccaa cgtaatttcc cgaagcgacg ccgaccagat cgatggaact caacaagcga
     5521 ttgaccatac cgccccaaaa attggtcgag ctctggcgca tcagctgctc ggattgaaaa
     5581 tggtcgtcgc ggttgatcac cgcttcaagg tactttctac gttccattgt cgaggcttcg
     5641 cgcagcgcct ggcgataggc ggcgagattt ttctcgcggt cctgaatcgt gttgttgacc
     5701 agatcctggg ctcgctccgt caggccggtg gtcgtgcgtt gctccttctg caggcgggcg
     5761 tcttctcttt tcaagaactc gagatggtct ttcagggttt cattgcggtt cgttaacgta
     5821 tcgatcagta ggtcgcggga gcgtttatcg acagcgtccg ggaagaattg cggcgcggcg
     5881 ggcgccgtgt cgaacgggtc atattcgggc atcggattta agattttttc gacgtccaat
     5941 tcacgcgcct gagcgcgaca aagggctgga gcgccgagca gcacggcgat gactgcggcg
     6001 acgccgaggc ggcaggttct atgcgttgtc atcgattgag cctcgtgagt ttttccatgt
     6061 aaccgcgagt cgcttcgatt ttggcctggg cttcaagttt ttcaatcacg ccgtcggttt
     6121 gagcgacttc gctgtagaac ttcaacgcgg atttggcgaa ttgttcgagt acattgagat
     6181 cgaatgccaa tccctcggga tcgtactggg cagcatagtc ttttgcaaga atcatgtaaa
     6241 agtcggcgaa gtcgagcaaa tgacgttgat agttcttgct ttgttggtgc ttggtgatca
     6301 gttggctata gcccacaatg gtcaactgat tgccttcttt caagcgaaag cgattggctt
     6361 cgacgaaagc gaccttggcg cgatcgatgc gctcttcttc cactcgcgcg aggaactcat
     6421 agggcgtgcc ccgatgtttt aggataagtc cgttccaagc cgccactttc tcgtcgagac
     6481 cggcgatgta tttgaatgga tcgtcgagcg gtatcgagcg gtcgaagatg cgcagaaagt
     6541 tatcgagaat ctcggctttt ttagcagctt cgctgccaag cgcaccctca gtctcagcga
     6601 tcttacggta gagcgccgct gcttggtcat attcgcgcaa gcgctcaagt gccaacgcgc
     6661 gattgaattg aacgatatcc gtaaactgac cgcgattctt ggtttcgtag tcttgcaggc
     6721 gggtcagcgt cgccttcatg atgttgatac ccgtgacatc cttggggatg gcaaatcgat
     6781 aggtatcttc cttagccaga cgctgaaagt ctctgacaat gtcgagcaga tcgccggccg
     6841 gcaaatagcg gccctccggc acggcagcgc gcgcccgatg tacgaatggc atggcgcaaa
     6901 aagaaatcac caaaagtata aaaaccggca aacgttttag gtgagcgatt ctcaagagcg
     6961 taacggcgcc acgaacgtgc taattatcgc gaagagcgaa gaaatttccg tactcgagct
     7021 attttccgag aaaaaccctc taagtcaagg cgcgcgaatg cgtccactgc gcttgaccac
     7081 gtcggagaag cagattaccc atgaaacatg gcatcgacaa ctcgccagac gttgttagct
     7141 ttcataatat ttgtgattca tctgaccggc tgcgctgaag gattgttcga accggcgcgc
     7201 ccggttaacc cgccggggcc gcttgcccta ccacagtcgc cgccgctgcc ggaatatcgc
     7261 ggcccggcga cgcggtcttt ttttatggga tttacgctct ggcccgcgga tctcagccgc
     7321 gacggcgtgg ccatcgctga ggactacgcg tacgcccatg gtgacattgt cgcggtgacg
     7381 ttcattagcg gtattccctg ggccgaagcg ttggacaaca agccctaccc gcgagatgtt
     7441 caagagcatc ttaactatcg cccgccagcg ggcaaaaaac tttttctctc gatctctccc
     7501 ttggataagg atcgccgcgg actggcacct tactggggcg acaccgatac tcagccgttg
     7561 ccgcagccgt ggaatcgcga gccgctcaac agcgcgcgag tcaagcgcgc gtatctgaac
     7621 ttcatcctgc gtgcggtgga aaccatgcgg ccggattttc tcgccatcgg tgtcgaatca
     7681 aatgtgctgt tatcgcgcaa ccctcagaag tggcaacagt tgaaagagct gcaccgtgac
     7741 acctacctgg cggtcaagaa gctgcacaaa gccttgccgg tatttttcac caccgacgtg
     7801 ctgcactaca aacggctggt agcggactcc aaggggtcgg agcaggaaaa ggaggtaatg
     7861 gatctgatgc gctacagcga tctgttcgcc atgagcgttt atccgcatat gagcttggaa
     7921 gtcgcccggc cggtgccggc gaactttctc gacttcgcaa cccggttcaa acggcccgtg
     7981 gcggtggccg agagcggcat ggcatcggcc gatgtcgaac tgcgcactta ttcaacgcta
     8041 ctacgcggct ccgatgccga ccagtaccag ttcaccgagt tgctgctgaa aaccgcggcg
     8101 cgcgattact acgaattcgt catcaatttc gccagtactg actccgatcg attggtcgca
     8161 cggcttcagc cgccgctcga tgatctggcg cgcctctggg catatacggg tatgcagacc
     8221 agcgacaagc gggcaaagcc cgcgtcagcg gtttgggatg cttaccggcg cgccaagtat
     8281 gaacaggcga agtggtggtg atatcccacg atcgcgttac tgcaatcgag caatgcaaat
     8341 gttttagcgt cgatcatcgc cggatcgata ccatgtcgtc cgtcacgcgg tgactatcaa
     8401 cgagtagaac agaaccaatt cgccgtactt accggcggcg aaatcatggg gtgtgccttt
     8461 ggggacgtga cagatgattc ctggccgcag tggcgtgcgt tttccattca cgcgcaggcg
     8521 gccgctgcct ttgatgacct gacagacgac gtctttgtca tcgtggatga tctgtttggc
     8581 gtccgagttt ttggatgggc ggaaggcaat aaggccgctg gtgaatgttt ctcccttaaa
     8641 gcagtcggca tagcgaattt cgtcggaaat ttttttgaga tctttcaggg ctgagttgat
     8701 cgtaatgatt ttcattgccg agtcgcgtga ccggggttga aacgggcgat tggcgttacc
     8761 ctgcaattcc ggatcttttt tgcgaatctt gcgttctttg cggctaactt cctggtttca
     8821 acctacttcg gttaccgcgg caagaatttc ctggatcgct tgctcggcct tatcgttggg
     8881 gacgcggctc gaaccgacaa ctttgtggcc gccgccgccg tagttcttga gtagctcgcc
     8941 gacattcaca ttggacgtcg ggttgaagat gttgtagccc accgagattg acgtcgtatc
     9001 ggcgcgctgg ctgtcgtaag ccactttgac ttggatattg cctttaggaa acaacgtgaa
     9061 caccaaaaat cgattgccgt ccgggatctc cttgacgccg cgcagatcgc aatagattac
     9121 gttgcctttg taacaagttc gctcgagcaa cagcttgcgc agtttctcct gctctcgttg
     9181 aatctgctcg cagcggcgct tgacgtccgc gtcgttcagc acttcttcta gggttgcatc
     9241 acgcagcaga ttgatcatct tcaaccagta gggctcatcg ccggcgtttc ggccgtcggt
     9301 ggtcatggag atcagcacat agccctgcgg attgagcacg tcgtcccgtt ccaactggcc
     9361 ggcgtcgact cgatcaacct cgtgcatcaa gtgctgcatg cgcgcagtgc cgaggaactc
     9421 ggtgatctgt tccggcgtgc tcccgttgcc ggttgcctgt tttctctcag ctgcttcctc
     9481 gtagtattca tacacgaccc gcgcagcact gggggcgagg cgaaagccgc cgcggccgag
     9541 aataatcggc ttttgaaaag tcggtgtggt gttggtgacg tgatggtcga accagagagt
     9601 gcagtgggga tgatagggga gattggtgat gatatcgccg ctgcgaatct cgaccaagcc
     9661 atcctgcatg aacttgggtt cgacgtatag atagtcgtcg atgttttcaa acttagtgat
     9721 taacgcgccg cagacgaggc cgtcgaagtc agcgcgggtg accagcctca tcggatcacc
     9781 tcgtcgacgg gcgtgaaggg cagattgaat gcgctggcga cgccttcata ggtaacctta
     9841 ccgttgaagg tgttcaagcc tttggccagc gctttgtttc tgacaagcgc ctgcggaaaa
     9901 ccgcgattgg ccaactccaa gccgtacgtc aacgtcgaat tcgtcagcgc gtaagttgat
     9961 gtattcggca cgatggccgg catgttggtg acgcagtagt gaaccagttc ttcttcgacg
    10021 taaattggat tggtgtgcgt agtcggccgc gaggtctcgg cgcagccgcc ctgatcgatg
    10081 gcgatgtcga caaacgcggc accggctttc attcttttta ctaacgcgcg gggtattagc
    10141 ttgggagctt gggcaccagg aatcagcact gacccgatga ctaaatcggc tccaacgacc
    10201 gcttcttcga cattggcccg attcgacatc agtgtcgtga catggccgcc gagaatatca
    10261 tgaacgtagc gcaacttgtt gggattgata tcgagaatgc tgacatgggc gcccatgccg
    10321 acggcgacct ggcaagccgc ggttcccgac atgccagccc caaggatgac aacgttggcc
    10381 gggcgcacac cagaggcgcc gcccaagaga atccctctgc cgccattctc cgcctgtaaa
    10441 caccaagccc ccacctgaat cgacaaacga ccagcgatct cactcatggg cgcgagtaac
    10501 ggcaacgacc cgtcgtccaa ctggatcgtt tcataggcga taccggtgac tttgcgctca
    10561 agcatcgccc gcgtgaccgc ttcatcggcg gccagatgaa gataggtaaa tagaatcaat
    10621 cccggccgca gaaacgcaaa ctcagactcc tgcggttcct tgaccttcat gatcatgtcc
    10681 gccttgtccc agacctgttt ggcgctgtcg acgatggtcg cgccggcggc ttgataaccg
    10741 gagtcagtga gaccgctgcc gctgccggcg cctctttgga tcaacacttg atgattacgc
    10801 gcgacaaagc cggcgacgcc ggtaggtgtc acggcgacac gattttcttc ggttttaatt
    10861 tctttgggga cgccgatgat catccgtaaa agctacgcga agcaaatgaa aaatgcaaaa
    10921 ttgataatcg agacgagttc aagacaattg acaatggaca accgctacga gatcagcgat
    10981 tcagggacca aatgaggatg gcccagccgc cgagaaaggc caagccgccg atcggcgtga
    11041 tggcgccgag ccagcgcgcg ccggtgaagc ttagcaagta taggctgccg gaaaaaattg
    11101 cgatgccggc gataaagcac cagccggctg tggtgaaatt gatatcgggc cagcgggtta
    11161 ttgcccaagc gaccccaagc agtgacagcg cgtgatacat ttgatagcgc acgccggttt
    11221 cgaaaatgtt gagcatgtcc ggcgaaattt tcgttttaag cgagtgcgcg ccgaaagcgc
    11281 cgagggcgac tccaatgaag gcggcgatgg cgccggcggt gaagaaaaaa cgctccatta
    11341 tttgcggtgc ttgcgcgcta gttcgaactc gtgagttgtt ggtctacagc gcgggcgcgc
    11401 tgcgattgaa tgatacgatc acctggcctt tttgcacgac aatctggcgg tagtctttac
    11461 gttgactcag cgcatcgatc agtttaacca gcggctggtt agcggtcaga tattctttgg
    11521 gcgcgccggc ggctttcatc gtttcgatct ctttttcttc gagattccaa cgcagcagaa
    11581 attcctcatc agaaacgtgc tcggcattca tctttttgcg cagttcggca accgagggtt
    11641 cgggcggagg tcgctcggcc cattccttgg cacgcggccg gtcgagaatc ttcgctttga
    11701 cctcgggcgc catcaactcg gcgccttctt tgccccaata gccgagcgcg tattgaataa
    11761 tttgatcggt aacttccttg tagcgctcgc cgacaatgac gttgatcgcc gcctgactgc
    11821 cgacgaactg tgacagcggt gtgaccataa tcggatagcc cagttcagcg cgcacctgca
    11881 tggtttctgc
//
LOCUS       DATLCH010000311         6726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10919561, whole genome shotgun sequence.
ACCESSION   DATLCH010000311 DATLCH010000000
VERSION     DATLCH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10919561"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VMZ02_09185"
     CDS             <1..823
                     /locus_tag="VMZ02_09185"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HUR72149.1"
                     /translation="GYFEGIRCYELADGRSAVFRLQEHMQRLADSGKILGFPLPYSVE
                     AMCKATVEVIRANKLRACYVRPLAFLGLGELGVYAPNNPVNVCIAVWPWGAYLGDDGI
                     RNGIRAKVSSYTRHHVNATMTKSKTSANYVNSVLAKAEVKKAGYDEAIMLDTEGYVSE
                     ASGENIFIVRNGKIKTTPLTSILPGITRDSIITLARDKGYEVSEERFTRDELYIADEA
                     FFTGTAAELTPIREVDDRQIGAGRRGALTEDIQNTFFDVIKGRSGEKYAAWLFLV"
     gene            875..1246
                     /locus_tag="VMZ02_09190"
     CDS             875..1246
                     /locus_tag="VMZ02_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HUR72150.1"
                     /translation="MVLASDPTLRVSMLPRDTNARGTIFGGVILSHIDLAGGIAAART
                     APQTFVTRAMRAVEFIAPVYVGDVVSFYTTVLREGSTSVTVRVSVEAERAKDPTVRVK
                     VTEAEVIYVAVDGTGKPIPLR"
     gene            complement(1321..2175)
                     /locus_tag="VMZ02_09195"
     CDS             complement(1321..2175)
                     /locus_tag="VMZ02_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HUR72151.1"
                     /translation="MQQLFFALSLLIVLAGCATANTVPTVTARDLASLSKPSTHRFLF
                     ASANGKAEGFVIRPRGKGPFPLMVLLHGHSLVGIGARRLLPAADTFASEVCYASLAIS
                     LPGYGDTQVPAGPTAEATRQVVNDAVALAKTLPWVDPERVYFYGFSRGAAVAAAMINQ
                     TSGVKGVLLHSGAYDLPRLYRDASSPWLRKLLNPDGDAAPQLQNLLNETDRWSGATLI
                     LHGAQDLLVPATQAHLLDQRLQALGKPHRLVLYPNYGHWLPLREIRSEALRFLIENGG
                     SACSTNDS"
     gene            2256..3170
                     /locus_tag="VMZ02_09200"
     CDS             2256..3170
                     /locus_tag="VMZ02_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HUR72152.1"
                     /translation="MKNRYFGWLLAAAAWVSVVACGDSAKSPAVLRVGFVPAEDAAQV
                     MQNAQPLVEILRKHLGMEIQPFVATDYTGVVEALRANKLDVAFLTPASYVLAKNETNV
                     RVVLKSERKGIPSYYAAIITRVDSGIKTLDDLRGKTFAFGDALSTTGNVFPRKMLKEH
                     GIDPVRDFKQVLYSGGHDATVLAVFNGKVDAGATYANSPDGEDTAWMRYLKSPEDVRK
                     IHAIAFSEPIPADNLVVNGKLDERLAQKIENVFIELSRDPEGKKMLRDLYQIDGFVSA
                     VDKDYDSVRHAFIEAGIPLKQALQKKQP"
     gene            3167..3943
                     /gene="phnC"
                     /locus_tag="VMZ02_09205"
     CDS             3167..3943
                     /gene="phnC"
                     /locus_tag="VMZ02_09205"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HUR72153.1"
                     /translation="MTILEIRGLQTSYNRSVPVLRGINLTVRQGDFVGVIGQSGSGKS
                     TLLRSINRLIDVDGGSIIAPASLFDGRTGPAVDILQLSNSELRQVRRRIGMIFQQFNI
                     VHRLSVINNVLSGGLGYQPAWRSTLRIFSREERRRALINLKRVGLLDHAYERADQLSG
                     GEQQRVAIARTLMQAPAIILADEPVSSLDPKLSRVVLDILKRVCREDGITAMVSLHTL
                     DLTREYADRVVGLKDGQIVFEGATEELTDSIVDAVYGPSD"
     gene            4150..5085
                     /gene="phnE"
                     /locus_tag="VMZ02_09210"
     CDS             4150..5085
                     /gene="phnE"
                     /locus_tag="VMZ02_09210"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HUR72154.1"
                     /translation="MAQVLEDVERNIFLEAPPRPRRGKKCFAALTIGAVLLAWSFHGA
                     KIRAGELVQGIPQIGQTLARMLPPDFSKITEAKHYYFPENLSWRELLLPAPLSEADAR
                     AKSRWWDNTFPQTVMGATLETIQMAMAGTFLALLVAFPLGFLAARNTTPHPLVYQGVR
                     LVLNLVRTIPDLALGLLFVAAVGLGAFAGTIALAIHTATVLGKLLSESVENIDEGVVE
                     AIRATGAGYSQILSFAVLPQILPDLISFTLYRFETNIRAASVLGLIGAGGIGYLMNTS
                     FRTFQYQEAAAIVLVLIGLVMLVDYLSSRLRNLVV"
     gene            5171..6022
                     /locus_tag="VMZ02_09215"
     CDS             5171..6022
                     /locus_tag="VMZ02_09215"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HUR72155.1"
                     /translation="MIRTVGLVAKYNEPKAAQMVRWLIPWLKKRGKKVCVENGLAPRG
                     AQSCTKKEMVVQADLIISLGGDGTLLNIAPLVERPEVPILGVNMGGLGFITEVAVGEL
                     KSVLTKTLAGDYEVEKRMTLEIRVISKKGRMRRFRVLNDAVITKGARSRIIDLETYVG
                     AEYLCTYRADGLIISTPTGSTAYSLAAAGPILEPTLGAIVLSPICPHTLTNRPIVVPS
                     DAPIHVTLRSFGDTVILSPDGQKGVRLNNGDKVEARDFGLPVSLIKLSSRSYYEILRE
                     KLKWGER"
     gene            6033..>6726
                     /locus_tag="VMZ02_09220"
     CDS             6033..>6726
                     /locus_tag="VMZ02_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR72156.1"
                     /translation="MLRELRIKNFAVIDEVSLELGPGLNIITGETGAGKSIILNALGL
                     VSGERGSSEIIRHKEDEATVEALFDTVPKLIEAKLADAGFELVDELVIKRILNRSGKN
                     RIYLNGSLCPLTLLAEIGGALVHIYGQHEHHSLLQPETHLNLLDAFAEVDALALGMKQ
                     QFAALSIAWNELNDTRDSLDKRRRERASLEAQAEEIARAQLHPGEEDELRGRKNILTH
                     SEKLNLGCREGEE"
BASE COUNT         1446 a         1883 c         1941 g         1456 t
ORIGIN      
        1 cggctacttc gaagggatcc gttgctatga actggccgac ggtcgctcgg cggtatttcg
       61 tctgcaagaa catatgcagc ggctcgccga ctcgggaaag attctgggct tcccgctgcc
      121 atactcggtc gaagcaatgt gcaaagcaac ggtagaggtg attcgtgcca acaagctgag
      181 ggcctgctac gtgcgaccgc tggcctttct gggtctcggc gaactcggtg tttacgcgcc
      241 aaacaacccg gtcaatgtgt gcattgcggt ttggccctgg ggcgcatacc tgggcgatga
      301 tggcattcga aacggtattc gcgccaaagt gtcgtcgtat acccgccatc acgtgaacgc
      361 cacaatgacc aaaagcaaga cctcggcgaa ttacgtcaat tctgtactcg ccaaggctga
      421 agtgaagaag gccggatacg acgaagcgat catgctcgac accgaaggtt atgtctcgga
      481 agcgagcggc gaaaatatct tcatcgtgcg caacggcaaa atcaaaacca ctccgctgac
      541 atccattttg ccagggatca cgcgtgattc gatcatcacc cttgcgcgtg acaagggcta
      601 tgaggtcagc gaagaacggt tcacgcgcga tgaattgtat attgccgatg aggcattttt
      661 caccggtaca gcggccgagt taacgcccat ccgcgaggtc gatgaccggc agatcggcgc
      721 cgggcggcgc ggcgcgctga ccgaagatat tcaaaatacc ttcttcgatg tgatcaaggg
      781 caggagtgga gaaaaatacg cggcatggct ttttctggtt tgattcccag ccgaagttga
      841 tctttagctt cgtcttcctt caatagattc acagatggtt ctcgccagcg atccgacact
      901 gcgggtatcg atgctgccac gcgacaccaa cgcgcgcggc acgattttcg gcggggtgat
      961 cttaagccac atcgacttgg ccggtggcat tgccgcggcg cgcaccgcgc cgcaaacttt
     1021 tgtcacgcgg gcgatgcgcg cggtcgagtt catcgcaccc gtgtacgtcg gcgatgtcgt
     1081 gagtttttac accaccgtgc tgcgcgaggg aagtacctcg gtgacggtac gcgtgagtgt
     1141 cgaagcggag cgtgccaagg acccgaccgt gcgtgttaag gtaaccgagg ctgaagtgat
     1201 ttacgtcgcc gtcgacggga ccgggaagcc gattccgtta cggtgagccg gttgccgcaa
     1261 ttttctgcgc atccgcccac tctgttggcg ccaaagaaac ctatagctgc tacgcaaaat
     1321 tcacgaatcg ttagtcgaac aggcggagcc gccgttttcg atcaggaacc gtaatgcttc
     1381 cgatctgatc tcacgcagcg gcagccaatg gccataattc gggtagagca ctaggcggtg
     1441 cggttttccg agagcttgca agcgttggtc tagtaagtgt gcctgtgtcg ccggaaccaa
     1501 cagatcctgg gcaccgtgca agatcagtgt cgctccagac catcggtcgg tttcattcaa
     1561 caaattctgc aattggggag cggcgtcgcc gtcaggattg agcagcttgc gcaaccacgg
     1621 gctcgacgcg tcgcggtaca gcctcggtaa atcataggct ccggaatgga gcagcacgcc
     1681 cttcacgccc gaggtctggt tgatcatcgc tgccgccacc gcggcaccgc gggaaaatcc
     1741 atagaaatag actcgctctg gatccaccca aggcagtgtc ttggcgagag caaccgcgtc
     1801 attcacaact tgccgggtcg cttccgccgt cggtccggct ggtacttggg tgtcgccata
     1861 gccgggcaag gagattgcga ggctggcata gcagacctcg ctggcgaacg tgtcagctgc
     1921 cggcaacaag cggcgcgcgc cgatgccgac caagctgtga ccatggagca acaccattag
     1981 cggaaacggc cccttgcccc tggggcggat gacgaaacct tcggctttgc cattggcact
     2041 ggcgaacaag aagcggtgcg tcgacggctt agacagacta gccagatctc gcgctgtgac
     2101 ggtggggacg gtgttggcgg tagcgcaccc tgctagaacg attaacaagc tcagcgcaaa
     2161 aaaaagttgc tgcacccagt tttagtaaca ttccaagtga aaaaatcaaa acgtttgcac
     2221 ccggtggacg cattcagtat cactgattgc ggctcatgaa aaatcgatat ttcggttggc
     2281 tattagcggc tgcagcatgg gtgagcgtag tcgcctgcgg tgattccgcc aagtcccccg
     2341 cggttttgcg cgttggtttc gtaccggctg aagatgccgc gcaggtaatg cagaatgccc
     2401 aaccattggt cgagatcctg cgcaagcatt tgggtatgga gattcagccc tttgtcgcca
     2461 ccgattacac cggcgtcgtc gaagcgttgc gcgccaacaa actcgacgtc gctttcttga
     2521 caccggcgtc ctacgtgctc gccaaaaacg aaaccaacgt tagggtagtg ctcaagtctg
     2581 aacgcaaggg cattccgtcc tactatgcgg caattattac ccgcgtcgat agcggcatca
     2641 aaacccttga cgacttgcgc ggcaaaacat ttgcgttcgg cgatgcgtta tcgaccaccg
     2701 gcaatgtgtt tccgcgcaag atgctcaagg agcatggcat cgatccggtg cgagatttca
     2761 aacaggtgct ttactccggc ggtcacgacg ccaccgtgtt agcggtgttc aatggcaaag
     2821 tcgacgccgg cgcgacctat gccaactcgc cggacggcga ggacaccgct tggatgcgtt
     2881 acttgaaaag cccggaagac gtcagaaaaa ttcatgctat tgctttctct gagccgatac
     2941 cggcggacaa cctcgtcgtc aacggcaaac tcgacgagcg cctagcacaa aaaattgaga
     3001 atgtttttat cgaactctcg cgcgaccccg agggcaaaaa aatgctgcgc gacttatacc
     3061 agatcgacgg cttcgtcagc gccgtcgaca aagactacga ctcggtgcgc catgcgttca
     3121 tcgaggccgg gatcccgctc aaacaggcgc tgcagaaaaa gcaaccgtga ccattctcga
     3181 gatccgcggc ctgcagactt catacaaccg ttccgtgccg gtgctgcggg gcatcaacct
     3241 aacagtccgg cagggggact tcgtcggtgt cattgggcag tccggctcgg gtaagtcgac
     3301 gctgctgcgc agtatcaatc gcctgattga tgtagacggt ggatccatta tcgctccagc
     3361 atcactgttt gatggccgaa ccggccctgc agtcgacatt cttcagcttt ccaattccga
     3421 gctgcgtcag gtgcgccggc gcatcggcat gatctttcag cagtttaaca ttgtccaccg
     3481 tttgtcggtt atcaacaacg tgctctccgg cggcttgggc taccagccgg cgtggcgcag
     3541 cacgctgcga attttttccc gtgaggagcg gcgccgcgcg ttgatcaact taaaacgggt
     3601 cggcctgctc gaccacgctt acgaacgggc tgatcaactt tccgggggcg agcagcagcg
     3661 cgtcgccatc gcacgaactt tgatgcaagc gccggcgatt attctggcgg atgagccggt
     3721 gtcgagtctc gatcccaaac tgtcccgtgt ggtcctcgat attctcaaac gggtctgccg
     3781 cgaagatggc atcaccgcca tggtgagttt gcatactctc gacttgaccc gcgaatatgc
     3841 cgaccgtgtg gttggactga aggacggaca aatcgttttc gaaggcgcta cggaagaact
     3901 taccgatagc atcgtcgatg cagtctatgg accgagcgac taaacgggat gcgccgctcg
     3961 caattgtctc attacaagtg ggtcaagcaa aaactgcgcg atgcgcaaat accatactca
     4021 ctctgggatg gaaacatgca gttggccgct tcaagtctcg acgtgaaatt gcctgggccg
     4081 atatgatatc tccgaagcta acagtatccg gcggtgtttc ggtgctaacg ctggtactcg
     4141 agtaacatca tggcacaagt actcgaagac gtagaacgta acatttttct cgaagcgcca
     4201 ccccgcccgc gccgcggcaa gaagtgcttt gctgccctaa cgataggcgc cgtgcttttg
     4261 gcctggtcgt ttcacggcgc aaagattcgg gccggggagt tggtccaggg cataccgcag
     4321 atcggccaaa ctttggcgcg catgctgccg ccggactttt ccaagatcac cgaggccaag
     4381 cactactatt ttccggagaa tctttcgtgg cgcgaattgt tgttgccggc gcctttgagc
     4441 gaggccgatg cgagagccaa aagccggtgg tgggacaata cgtttccgca gacggtgatg
     4501 ggcgcaacac tcgaaactat ccagatggca atggccggaa cgtttttggc attgctggtg
     4561 gctttccctc tgggatttct cgctgcgcgg aatacgacgc ctcatccact cgtctaccag
     4621 ggtgtacggt tagttctgaa tttggtgcga acgattcccg atcttgcgtt aggattgctt
     4681 tttgtcgccg ctgtcggttt aggcgcgttc gccggcacga tcgctctggc gattcatacc
     4741 gccacggttt tgggcaaact gttatcggag tcggtggaaa atattgacga aggcgtggtc
     4801 gaggcgatcc gcgccactgg cgccggttac tcgcagatac tctccttcgc ggttttgccg
     4861 cagatcttac ccgatttaat ctcgtttaca ctctaccgct tcgaaaccaa catccgcgcc
     4921 gcctccgttc tcggactcat cggcgccggc ggtatcggct acctgatgaa caccagtttt
     4981 cgcacttttc agtatcaaga ggccgctgcg atcgtgcttg tcttgatcgg cttggtcatg
     5041 ctcgtcgact acttgagctc ccgcctacgt aatcttgtcg tttgactaca cgctatgtga
     5101 ggcgcgctaa cgctgtcatg gacttctttt ttggccatgt gatactctcc ccgttggagc
     5161 aacgtctcga atgattcgca ccgtaggact cgtcgctaaa tacaacgaac ccaaagccgc
     5221 gcagatggtc cggtggctca ttccctggct taagaagcgc ggcaagaaag tctgcgtcga
     5281 aaatggcttg gcgccgcgcg gcgcgcaatc ctgcaccaag aaggaaatgg ttgttcaggc
     5341 tgatctgatc atctccctcg gcggcgatgg cactctgctc aacatcgcgc cgctggtcga
     5401 gcggccggag gtgccgattc tgggcgtgaa catgggcggc ttgggcttta tcaccgaggt
     5461 cgcggtcggt gaattaaaat cggtcctgac taaaactctc gccggggatt atgaagtcga
     5521 aaagcgcatg accttggaaa tccgggtcat tagcaagaag ggtcgtatgc gccgcttccg
     5581 cgtcctaaac gacgcggtga tcactaaggg tgcgcgcagc cggatcatcg acttggaaac
     5641 ctatgtcggc gcagaatatc tctgcaccta tcgagccgac ggattgatta tctcgacgcc
     5701 caccggttca acggcatact cactggccgc ggccgggccg atcttggagc ctacgttggg
     5761 cgccattgtg ctatcgccga tttgtccgca cacgctgacc aatcgaccca ttgtcgtgcc
     5821 gagcgacgcg ccgattcacg taaccctgcg ctcctttggt gacacggtga ttctcagtcc
     5881 ggacggtcag aagggggtgc gcctcaacaa cggtgacaaa gtcgaggcgc gcgatttcgg
     5941 tttacccgtt tcgctgatca agctgtcgtc gcgcagttac tatgagatac tccgcgaaaa
     6001 gctcaagtgg ggggagcgct agttaacggc ctatgttgcg cgagctgcga atcaagaact
     6061 ttgcggtgat cgacgaagta tcgctggagc tcgggccggg actgaatatc attaccggcg
     6121 aaacgggtgc gggcaagtcg attatcttaa atgccttggg gcttgtttcc ggtgaacggg
     6181 gcagttcgga aatcattcgc cacaaagaag atgaggcgac cgtggaggca ttgttcgaca
     6241 cggtgcccaa actcatcgaa gccaagctcg cggacgccgg ctttgaactc gttgacgagc
     6301 tggtaattaa gcgcattctc aatcgctccg gcaaaaaccg catttatctc aatggcagtc
     6361 tctgtccgct cactttgctg gcggaaattg gcggtgcttt ggtgcatatc tacggccagc
     6421 acgagcatca cagcctgttg cagccggaga cccatctgaa cctgctcgac gcattcgccg
     6481 aagtcgacgc gctggcttta gggatgaaac agcaattcgc cgcgctgagc attgcctgga
     6541 acgaactcaa cgacacccgt gattccctcg acaaacggcg ccgtgagcgc gcgagtcttg
     6601 aagctcaggc cgaggagatc gcccgggctc agctccatcc gggtgaagaa gacgagctgc
     6661 gcggacgtaa gaatattttg acccattcgg aaaagctcaa tctgggctgc cgcgagggcg
     6721 aagagc
//
LOCUS       DATLCH010000312         4266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3487249, whole genome shotgun sequence.
ACCESSION   DATLCH010000312 DATLCH010000000
VERSION     DATLCH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487249"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VMZ02_09225"
     CDS             <1..867
                     /locus_tag="VMZ02_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72157.1"
                     /translation="RGRALGIYQMSNAVGYAVGPLLGGMLIDVLSWRATFLFRVLPAL
                     LLAALAVGKLPPHEQARERQRGISHSPILSVARRPSFIIANLLSVLANCARFAIGLLL
                     PYYVINVLRYPAAAGGMLMLTTYLLTILAAPLAGKLSDRLGTVRLSSLGLTVQGLGLW
                     MLSRLDGQTNYLSLAFALAVVGLGLGIFEAPNMSFIMGSIPRSRQGVAGSVAAMMRPL
                     GIVIGATGWSLLFDWRRQYYSPLAADLNSVAINGMLPALQDVFLSAAGVCFAALLLSL
                     CRGPEPDRSKTE"
     gene            974..1303
                     /locus_tag="VMZ02_09230"
     CDS             974..1303
                     /locus_tag="VMZ02_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72158.1"
                     /translation="MAQRITFVIFGGGGLALLYVGVTQYFLQRRLMANAIAIEVEIIK
                     SEIFQSVSADTDQRLNRNNSTTTFRPGIRFRYTFAEREYESDLLRPTIIVRGYASAEN
                     AAKQLAP"
     gene            complement(1434..2690)
                     /locus_tag="VMZ02_09235"
     CDS             complement(1434..2690)
                     /locus_tag="VMZ02_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72159.1"
                     /translation="MKNYIGSFFLAVCFVASAPTVHGAQAPVQLPDGEGKPLVQGLCA
                     TCHELNLIPRSSGYTRAGWQELIGNMINIAGTPAGDTVSQYLATHFPPNTNLQPRLIP
                     GPVSISFREWKVPQLGQRTRDPVESADGSIWYCGQYGNIVGRINPKTGEVWEYTLPDK
                     ALPHSVTPDREGNIWYTGNGNGTVGKLDPKSGRITEYKMPDPAAKDPHTAVFDEKGRM
                     FFTLQVSNMIGRLIPSTGEIKLITLTRKNSRPYGIKVAANGSLWVSCNGSNCILNVDP
                     ETLAVREFNLPEDTTVRRLDIASDGTIWYVNSGLGRLGRMDPKTGQYKEWASPSGPKS
                     HPYAIAVVDDIVWYNESTQRPDALVRFDPATEKFQSWPIPSGSIYGGILRHMRPTRAG
                     NLLIHQTSTNRIYEVNVKPTDRRVAN"
     gene            complement(2773..3723)
                     /locus_tag="VMZ02_09240"
     CDS             complement(2773..3723)
                     /locus_tag="VMZ02_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72160.1"
                     /translation="MHAAGANCGCAADRLRIGYSGLSPATAMLWVTQEGKLFEKNGIS
                     ADVLFLRNNLGQSAMIAGEIDMCSYSASLMAPARVQGADLVMIVSFQQKLNYRLVVRP
                     EIRTLADLKGKVLGVTRYGTVNDSTMRLLISKLGLNPERDVTLIQVGDSAPVVATSLI
                     AGKTFDGALLQPPYYNKAVESGFRVFANMEEMDIPFQQVGLNTTQRLIAKNPDTVRRV
                     VKSVVEGVQLIRTNPAVAKRAIALYMQIKDEKVLEESYQQLKGTAEIKPYPSAEGFKT
                     IVDDLAKKLPAARTANPRDFMDARFIQDLDKSGYIDGLYR"
BASE COUNT          872 a         1203 c         1200 g          991 t
ORIGIN      
        1 cgcggtcgcg cgctgggaat ttatcaaatg tctaacgccg tcggttatgc cgtcggtccg
       61 ttgctcggcg gcatgctgat cgacgtcttg agttggcgag cgactttttt gttcagagtg
      121 ttgccggcgc tgttgctcgc cgctctggcg gtcggcaaac ttcccccgca cgagcaagcg
      181 cgggaacgcc aacgcggcat atcacactcg ccgatattga gcgttgcccg ccggccatca
      241 ttcatcatcg ctaacctgct gtcggtgttg gccaactgcg caagattcgc catcggcctg
      301 ttgctgccct actacgtgat taatgtcttg cgctatccgg ccgccgccgg cggtatgtta
      361 atgctgacaa cttatttact gacgatcctg gccgcgccgc tggcgggaaa attgtccgac
      421 cgcctcggca ccgtgcggct cagttcgctg gggctcaccg tgcaagggct cggtctgtgg
      481 atgctcagcc gcctcgatgg acaaaccaat tatctttcgc tggctttcgc tctcgcagtc
      541 gtgggtttgg gcctcggcat attcgaagcg cccaacatga gttttatcat gggttccatc
      601 ccccgttcgc gacaaggcgt ggctggcagc gtcgccgcca tgatgcgccc gctcggcatc
      661 gtcataggcg ccaccggttg gagcctcctc ttcgactggc gccggcagta ttattcgcca
      721 ttggctgccg atctaaactc ggtggcaatc aacggcatgc tgccggcgct gcaagatgtt
      781 ttcctcagcg ccgccggggt gtgtttcgcg gcgttgctcc tgtccctctg ccgtgggccc
      841 gaacccgaca ggtcaaaaac cgagtgacgc ggagggttta aattcgctaa cgagagcaag
      901 acactctcgg ttgaataaat tcactggcct cagcatgatg cctgtgctca ggcgcgactg
      961 caggaggatt tcggtggccc aacggatcac atttgtaatt tttggcggcg gcgggctcgc
     1021 actgctttac gtcggcgtga cgcaatattt tctccaacgt cggttgatgg ccaacgccat
     1081 tgccatcgaa gtggaaatca tcaaatcgga aatcttccaa agcgtatcag ccgacacgga
     1141 tcaacgactc aatcgtaata attcgacgac cactttccgc cctgggattc gttttcgcta
     1201 tacgttcgcc gaacgtgaat acgaaagcga cctgctgcgc cccaccatta ttgtccgagg
     1261 ctatgcttca gccgagaatg ccgccaagca gctagcgccg tgagagtttg ccggcccggc
     1321 agtgttcata atcataggca atgtcctgcc gccgctcgcc tggatcgttg gcaaatacgt
     1381 ctgactcgcc gctgtaagtg cccatcgccg cgtcatgtca cacccgttca gtttcaattg
     1441 gcgacccggc gatcggtagg ttttacgttc acctcgtaga tccggttggt gctggtctgg
     1501 tggatcaaaa gattgccggc acgggttggc cgcatatgtc tgagaatgcc gccgtaaatg
     1561 ctacctgacg ggatcggcca gctttggaac ttctcagtcg cgggatcgaa gcgaaccaag
     1621 gcgtcggggc gctgggttga ctcgttgtac caaacaatgt cgtcgaccac agcgatagcg
     1681 taaggatgtg atttgggtcc gctcggtgat gcccattctt tgtattggcc ggtcttcggg
     1741 tccatgcggc cgagccgtcc caggccggaa ttgacgtacc agatcgtgcc gtcactggcg
     1801 atgtcgaggc gacgcaccgt ggtgtcttcg ggaagattga attctcggac cgctaatgtc
     1861 tcgggatcga cgttgaggat acagttgctg ccgttgcagg aaacccacaa cgaaccgtta
     1921 gccgccacct tgatcccgta aggccgagag ttttttctcg tcagcgtgat caatttgatc
     1981 tcgccggtcg acgggatcaa gcggccgatc atgttgctca cctgtagggt aaaaaacatg
     2041 cggccttttt catcgaacac cgcggtgtgg ggatccttag cggcgggatc gggcattttg
     2101 tattcggtga ttctgccgga tttcggatcg agcttaccga cggtcccatt gccattgccg
     2161 gtataccaaa tattcccctc acggtccggc gtgacgctgt gcggcagcgc tttgtccggc
     2221 agcgtgtact cccaaacttc gccggttttc ggattgatcc ggccaacgat attgccgtac
     2281 tgcccgcaat accagatcga tccatcggcg ctttccacgg ggtcgcgggt gcgttggcca
     2341 agttggggaa ctttccattc gcgaaaactt atcgaaaccg gaccgggtat caaccttggt
     2401 tggagatttg tgttgggcgg aaaatgcgtc gccaggtact gtgacacggt gtcgcccgcc
     2461 ggcgtaccgg cgatgttgat catgttaccg atcaattctt gccagccggc gcgcgtgtat
     2521 cccgaactgc gcggtatcag atttagttcg tgacatgtgg cgcacaggcc ttgaacgagc
     2581 ggcttgccct cgccgtcggg caactgcacc ggagcttggg cgccatggac cgtcggcgca
     2641 gacgcaacga aacagaccgc caaaaagaaa ctgccaatat agtttttcat agcgtgctcc
     2701 tattcgcttc gttgaatgtc aactggatgc gtcactctag cactttttta ccctcactgt
     2761 cgtaaaggaa acttaccgat acaacccatc gatatacccc gacttatcca ggtcctggat
     2821 gaaccgcgca tccatgaagt cgcggggatt ggcggtgcgc gcagccggga gttttttagc
     2881 caaatcgtcg acaatggtct tgaagccttc tgcggaggga tagggtttga tttccgcggt
     2941 gcctttcaac tgctggtacg actcctccaa aaccttctca tccttgatct gcatgtaaag
     3001 agcgatagcc cgcttcgcca ccgcgggatt agtacgaata agctgcacgc cttcaacgac
     3061 cgacttgacg acgcggcgaa cggtatcggg gttttttgcg atgaggcgct gggtcgtgtt
     3121 caggcccacc tgttgaaatg ggatatccat ttcttccatg ttggcgaata cgcgaaaccc
     3181 ggactcgacg gccttgttgt agtaaggcgg ctggagcagc gcgccgtcga aagtcttgcc
     3241 ggcaatcagc gacgtagcga ctactggagc cgagtcgcca acttgaatca acgtgacgtc
     3301 gcgctcggga ttcagtccga gtttgctgat gagcaagcgc atggtcgaat cgttgacggt
     3361 gccgtaacgg gtgactccga gcaccttgcc cttgagatcc gccagcgtgc ggatctccgg
     3421 ccgtacgaca aggcggtagt tgagcttttg ttgaaaactc acgatcatca ccaggtcggc
     3481 gccttgaaca cgcgcaggcg ccatcaagct cgccgagtag ctgcacatgt cgatttcccc
     3541 ggcgatcatc gcggactgcc cgaggttgtt gcgcagaaac agcacgtcgg cactgatacc
     3601 gtttttctcg aatagcttgc cttcctgtgt aacccagagc atcgcggtcg ccggactcag
     3661 gccggaatag ccaatacgaa gcctatcggc ggcgcaacca cagtttgcgc cagcagcatg
     3721 aataacaaaa agcttatgtg aattattcgt ttcacgatta gttccacgtg agtcgaccga
     3781 ggtaaggccg caactgtttc ttcacccccc tcaatcaaac caatcacagt cttaacacag
     3841 agaacgttgg ccaagaaaga ccaaaccatg gcctaagtaa tgggatctca cgggcgaatg
     3901 agacttccga agcgcaatcg gacagctccg caatcggaca gctctcggat acatgcggcg
     3961 gcgtgcctca catgaccgag gactaaatac gttactctgc ctaatgtgac gacgaaaatc
     4021 gtcatgccgg cgaaggccgg caccaggagt tgggacggga cggggatgga taccggcgtt
     4081 cgccggtatg acagaacgtt attgccattt cttcggcccg atcttcggta gtggtccagt
     4141 ttgagtcggg gcagaagtat ttcaatctga gaaagatttc tcgcttcgct cgaaatgaca
     4201 gcagaatggt cgcctttgtc atctcgaacg agttgagaga tctatatcta actaaaccgc
     4261 tactca
//
LOCUS       DATLCH010000313         8173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7878966, whole genome shotgun sequence.
ACCESSION   DATLCH010000313 DATLCH010000000
VERSION     DATLCH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7878966"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..833)
                     /gene="rimI"
                     /locus_tag="VMZ02_09245"
     CDS             complement(291..833)
                     /gene="rimI"
                     /locus_tag="VMZ02_09245"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HUR72161.1"
                     /translation="MASIDLSSKANLATTEMPQEETSCGFFVCGKQVLFRPMTKDDLE
                     EVMVIERSAYRYPWSLGFFLQELQTACARSIVADIDGRIGGYVLFWLLPGSIDVHNLA
                     IAPELRRLGIARMLLRQVTTAAQVQSATRVTLEVRQSNQAAQRLYASLGFIKTGLRRG
                     YYSDNGEDAFTMALELDESI"
     gene            complement(843..2174)
                     /locus_tag="VMZ02_09250"
     CDS             complement(843..2174)
                     /locus_tag="VMZ02_09250"
                     /inference="COORDINATES: protein motif:HMM:NF033683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HUR72162.1"
                     /translation="MATMITADCINCGACEPECPNNAISQGDPVYVIAPDLCTECVGF
                     HDYEACAAVCPVDVCVTDPQNIETEDVLIARARTLHPELTIGDNFQSRFRKGVAAVAP
                     AVPASPLTTPTAPVATIKPAAAPVIAKAPPPPVASAKPVSVPAQVKKEPRPKKNFPHE
                     LPVSFDEAMKQFRSGGSTQSGWFRAAVILAQPLIGALPHTTKKHLEHAVQSPFFTAAG
                     STAVNIVHNAVLYPLIAAVIGIAWHGDPGFIFDKGVNIYLLVGLLVAVLEGIFRLKDG
                     LFRAKPSEEMEFPPAFYGVPLALVANSLLSKRFGLVREMPIPVEGFYSKGFVDKRERE
                     RRYGNIYTVEEFGGAFLMRMEFPRWLPEIGIKNRDQLSDEMPDYDYDLLLQDGQLIVK
                     GKCTDEKVRKITSSVGAFPPEFTSVIAFRERISGFVHQFHNKNLEVLLVKA"
     gene            2285..2360
                     /locus_tag="VMZ02_09255"
     tRNA            2285..2360
                     /locus_tag="VMZ02_09255"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2318..2320,aa:Cys,seq:gca)
     gene            complement(2415..2678)
                     /gene="grxC"
                     /locus_tag="VMZ02_09260"
     CDS             complement(2415..2678)
                     /gene="grxC"
                     /locus_tag="VMZ02_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HUR72163.1"
                     /translation="MAQITIYTTNWCPYCHGAKALLRSKSVAFDEIDVTDEPEKRKEM
                     ERLSHRRTVPQIFIDGRAIGGYDEARLLDATGELDKLLGIVRN"
     gene            2762..3358
                     /gene="coaE"
                     /locus_tag="VMZ02_09265"
     CDS             2762..3358
                     /gene="coaE"
                     /locus_tag="VMZ02_09265"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HUR72164.1"
                     /translation="MKLVGLTGGIASGKSTVARILKDLGAAIVDADALSREVVSPGQD
                     GWHEIVATFGPEVLQADQTLDRQKLRKLIFNNPEARKKLEAIIHPRVRALAEERIREY
                     GDAGYAIVVYEVPLLFEGGLQEWLRPVILVASDVNIQRQRLQQRDGLDAEAAQKHIDA
                     QMSLVEKRRLADYVIENNGTLADLEAQVRAVIAEIQAT"
     gene            complement(3351..3911)
                     /gene="efp"
                     /locus_tag="VMZ02_09270"
     CDS             complement(3351..3911)
                     /gene="efp"
                     /locus_tag="VMZ02_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HUR72165.1"
                     /translation="MVQATQLRPGMIIIHEGDLYRVTAVHHLTPGNKRGFMQTKMKSL
                     RTGVGTEYKFRSEDRVDQAILDNRQFQYLYAEGEMHTFMDTENYEQITLSAEDIGDLL
                     NYLLPNSMVGIEFHDGKPVGINPPSTVDLQVVDTEPSMKGATASASYKPAKLETGVMV
                     LVPPFVQVGDKVRVDPTDGSYQERVK"
     gene            complement(3984..5243)
                     /locus_tag="VMZ02_09275"
     CDS             complement(3984..5243)
                     /locus_tag="VMZ02_09275"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HUR72166.1"
                     /translation="MATLEWRSETNEMAVQQFDVAASKLGVDPNLANRLRRPDRAMIV
                     SVPTRMDDGRVHVFTGYRVQHNDVLGPFKGGIRYHPAVNLGEVSALAMWMTWKCSLVG
                     LPLGGAKGGIACDPAELSRHELQSMTRRFTAELLNFIGPEVDVPAPDMGTNEQVMAWI
                     MDTYSQHKGYAVPEIVTGKPVAIGGTVGRREATGRGVVYTIIEAAKHLNIDLAKCTAV
                     VQGFGNVGSVAAKELAGIGVKVIGIADRTGGFVDTKGLPVDQLLEVADKNHSLEGCPY
                     GDKITNAELLELKCDILVPAAMEMQITKNNAARIQCRVLAEGANGPTTPEADAILRDK
                     GIFVIPDILANAGGVVVSYFEWVQDLQNFFWTEDEVTKKLRDILVKAFHEVLEMSQKQ
                     KVDMRMAALMIGIERVTKAMLWRGLYA"
     gene            complement(5280..5624)
                     /locus_tag="VMZ02_09280"
     CDS             complement(5280..5624)
                     /locus_tag="VMZ02_09280"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HUR72167.1"
                     /translation="MADCLFCGIADGTIKANLIYQDDAVLAFTDIAPKAPVHILIIPR
                     KHIASVLDIEAVDSGVIGRIFEVAGRLAREQGIADRGFRLVVNSGRDAGQTVFHLHYH
                     LLGGRQMTWPPG"
     gene            complement(5660..6031)
                     /locus_tag="VMZ02_09285"
     CDS             complement(5660..6031)
                     /locus_tag="VMZ02_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR72168.1"
                     /translation="MTAAKKPSGKLVGTVGELPHGKSKKFTMKRAGREFEALLVNFDG
                     THFAYINRCPHTGITLDWVNNQFFSSDNRYLMCATHGAVFEPPTGECIWGPCVGLSLQ
                     SLPIETVDGQIYAKLPGAADE"
     gene            complement(6047..6526)
                     /locus_tag="VMZ02_09290"
     CDS             complement(6047..6526)
                     /locus_tag="VMZ02_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR72169.1"
                     /translation="MALTIYLSLIFLVLYLIGLMVRRRIFVRDRNRMAAESRTGGRVV
                     ARVNEVQPGAVKKFWLICQQYRLDAILINDQGNYYAYVNRCRHMPTPLDFVRDEFFSD
                     DGRYLQCYTHGALYEFASGLCVSGPCKGESLYRLPVQIDRGEVLVGCPEGDLRPMGE"
     gene            complement(6546..6905)
                     /locus_tag="VMZ02_09295"
     CDS             complement(6546..6905)
                     /locus_tag="VMZ02_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72170.1"
                     /translation="MPNSLLIGQRTIMRQCIYCGERAGFFGRVCADCKLLLARVEELR
                     GKVGFVQFLDGLAETGVAKDKILKFLDADPDGKGSVRDQVTAEMTSELMKVMGISGRQ
                     TAPEVKRIRNSVNKQSD"
     gene            complement(6895..7698)
                     /locus_tag="VMZ02_09300"
     CDS             complement(6895..7698)
                     /locus_tag="VMZ02_09300"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HUR72171.1"
                     /translation="MTIDFQFSEELGIAEQAARDAGAAIMGLFRGKYDVREKSKNNPV
                     TTADLEANRIIRERIADAFPDDGWLSEEDADSERRLTLPRVWVVDPIDGTKEFIEGVP
                     QFAISIGFVVDGVAQAAVVYNPAQGEFFTGAAGHGAFLNDNSIHVTSREQIDGARLLV
                     SRSEPAKRFQVFVDRCAIEPVGSIAYRLAKVAGGDGDGTLTFRSIHEWDICAGVLMVE
                     AAGGAVVDGDGHAMRFNRQPAKHRGVVAASAALTPGLQGLWAAAMNGAK"
     gene            complement(7775..>8173)
                     /locus_tag="VMZ02_09305"
     CDS             complement(7775..>8173)
                     /locus_tag="VMZ02_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HUR72172.1"
                     /translation="YGGVWPVHCVQNTLGAEFHSALRLSSTATVLSKGTMADADSYSG
                     FDALDEHGNDLAVLLRARGVEHIFVGGLATDYCVRQTVLDGLQQGFDATVLLDAIRGV
                     DLVPGDSARALEEMSLAGARIIHGVEELPC"
BASE COUNT         1929 a         2358 c         2092 g         1794 t
ORIGIN      
        1 cggagaaaat aaggttgggt gagctggaga ttcggccgga tagcaaagcc gacgtcggta
       61 gaaaaggttt tttgagtcat gcggttcgtc aaatcccgcg tccgggacta caaaaaaaac
      121 agcggtcact aagaccgcgt cacctgtaac cagcaagtaa gttatacaac agacgcaaag
      181 gtcgcgtcaa gctcgacgca aaattgacta aaacatcaac agccttttcg ataatttacg
      241 tctaaaattt cgaaccgtaa ttggatttaa gattcctaaa ccagatgaaa ttaaatcgac
      301 tcgtcaagtt ccagagccat tgtaaatgca tcctcgccat tgtcagagta gtagcccctg
      361 cgcagccccg ttttgataaa tcccagtgat gcataaagcc tctgagctgc ttggtttgac
      421 tggcgaacct caagtgtaac acgagtagcg gattgcacct gagccgcggt cgtaacttga
      481 cgcagcagca tacgtgcaat accgaggcga cgtagctctg gagcaatcgc caggttatga
      541 acgtcaatag atcctgggag cagccaaaac agtacgtagc ccccgatcct accgtcgata
      601 tcggcaacaa tcgagcgcgc acatgcggtt tgcagttcct gaagaaaaaa acccaaactc
      661 cagggataac gataagccga gcgttcgatc accataacct cttcaagatc gtccttagtc
      721 ataggcctaa agagtacctg cttgccacac acaaaaaagc cacaggaagt ttcttcctgt
      781 ggcatctcgg tcgtggcgag atttgctttt gatgataaat cgatcgaagc cactctattc
      841 aattaggctt taacgagcag tacttctaaa tttttattat gaaattgatg aacaaaaccg
      901 gagatccgtt cgcgaaatgc aatcacactg gtaaattccg gcggaaatgc gccgacgctc
      961 gaggttattt ttcggacctt ctcatccgta cactttccct taactatcaa ctgaccgtct
     1021 tgcagcaaca agtcgtaatc gtaatccggc atttcgtccg acagctgatc acgattctta
     1081 atgccgatct ccggaagcca acggggaaac tccatgcgca ttagaaaagc cccaccgaac
     1141 tcctcaactg tatagatatt gccgtaacgc cgctctcgtt cgcgcttgtc gacaaaccct
     1201 ttggagtaaa aaccctcaac cgggatcggc atctctcgga ccagcccgaa gcgctttgat
     1261 aaaagtgaat tcgccaccaa cgcgagagga acaccgtaga acgcgggggg gaattccatt
     1321 tcttccgaag gcttcgctct gaacaaacca tccttcaggc gaaatattcc ttccaataca
     1381 gcaaccaata acccgaccag caagtaaata ttgacgccct tgtcgaaaat aaatcccggg
     1441 tcgccatgcc acgcgatgcc aataacggca gcgatcaagg gataaagcac cgcattgtga
     1501 acgatgttca ccgctgtgct gccggcagcc gtaaagaacg gactctgcac cgcatgttcc
     1561 aaatgctttt tggtagtgtg cggcaacgcg ccgatcagtg gctgcgcgag aatcacagcg
     1621 gcacgaaacc atccggactg cgtcgagccg ccggagcgaa actgcttcat cgcttcgtcg
     1681 aaactcaccg gtagctcatg gggaaagttc ttcttcggtc gcggttcttt ctttacctgc
     1741 gctggtaccg acactggctt tgccgatgcg actggtgggg gcggcgcttt agcgatcacg
     1801 ggagctgcag caggttttat tgtagcaacc ggtgctgtcg gggtcgtcag tggcgaagcc
     1861 ggaaccgccg gagcgacagc ggcgacgcct ttgcgaaaac gcgattgaaa attatcgcca
     1921 atcgtaagct caggatgtaa cgtgcgcgcg cgagcgatca acacatcttc agtttcaata
     1981 ttctgcggat cggtcacaca gacatcgact gggcacaccg cagcacaggc ttcgtaatca
     2041 tggaagccca cgcattccgt acagagatca ggggcaatga cgtaaacggg atcgccctgg
     2101 ctaattgcat tattgggaca ttcaggttcg catgccccgc aattaatgca gtcggccgtg
     2161 atcatcgtcg ccatggataa actccttgta acctttcaat ttgcttactt gtttcgtagc
     2221 gggaagtcaa taaatccgcg cttattgcat gtcgcaaaca gaacatgtta cctatttcga
     2281 cgagggcggc gtacccaagt ggttaaggga gaggtctgca aaacctcgat gcagcggttc
     2341 gaatccgctc gccgcctcca cttcttccgc ggaatatagc cgtaccgtgc aaccacttcg
     2401 acagcggcgt aatctcaatt tcgaactatt cccaacaatt tgtcgagttc gccggtagca
     2461 tcaagcagcc tagcttcgtc ataaccgccg atcgcacgcc cgtcgatgaa aatttgcggc
     2521 accgtacggc ggtgagacag tcgttccatt tctttgcgtt tttccggttc gtcggtaacg
     2581 tcgatttcgt caaacgccac agatttggaa cgcagaagcg ctttcgcacc gtggcagtac
     2641 gggcaccagt tagtcgtgta gatggttatt tgggccatgc tgattttctc caatgttaat
     2701 ctctttaatc attttaaaac ttttgcttga aagcctctag tgattttgta attactgtcg
     2761 cgtgaaactc gtcggtctca caggcggcat cgcatccggc aaaagtacag tagcgagaat
     2821 tttaaaagat ctcggtgcag cgatcgtcga tgccgatgcg ctttcccgtg aagttgtctc
     2881 tcccggtcaa gatggttggc atgagatcgt cgcgactttt ggccctgagg tactccaggc
     2941 tgaccaaact ctcgatcgcc aaaagcttcg aaaacttatc ttcaataatc ctgaagcacg
     3001 caaaaaactc gaggcgatta ttcatccccg tgtccgcgct ctcgccgaag aacgcattcg
     3061 cgagtatggc gacgccggat atgcgatcgt agtttacgaa gtgccgctgc tgtttgaagg
     3121 agggctacag gaatggctcc gaccggtgat tttggtggcg tcagacgtca atattcaacg
     3181 ccagcgattg cagcaacgcg acggtctgga cgccgaagcg gcgcagaaac atatcgatgc
     3241 gcaaatgagt ttggtagaaa aacgacggct ggccgactac gtcatcgaaa acaacggcac
     3301 attggcagat ctcgaagcgc aagtgcgcgc cgttatagcc gagatccagg ctacttaact
     3361 ctttcctgat acgatccatc ggtcggatcg actcgcactt tatcgccaac ttgaacaaac
     3421 ggcggcacca acaccataac gccggtctcc aattttgccg gtttgtaaga agcgctcgcg
     3481 gtagccccct tcatcgacgg ctcggtatcg actacttgca agtcgaccgt cgacggcggg
     3541 ttgatcccga ctggtttgcc gtcgtggaac tcgatgccga ccatggagtt gggcaataaa
     3601 taattcaaca gatcgccgat gtcctcggca ctgagcgtga tctgctcgta attttcggtg
     3661 tccatgaaag tgtgcatttc gccttcggcg tagagatatt gaaattggcg attgtccaag
     3721 atcgcctgat cgacgcggtc ttccgagcgg aatttatatt ccgtgccaac gccggtgcga
     3781 agactcttca tcttggtctg cataaagccg cgcttgttgc cgggggtcaa gtgatgcacc
     3841 gcggtgacgc gatagagatc accttcgtga attataatca tgcccggccg cagttgagtt
     3901 gcctgaacca tttcaccctc ttagtcgaaa aagtatttga gttgttaaca gtttcccgcc
     3961 ggcgcttcaa tgcaagcggt cgcctaggcg tataggccgc gccagagcat cgctttggtg
     4021 acgcgctcga tgccgatcat gagagcggcc atacgcatgt cgactttttg cttttggctc
     4081 atctcgagca cttcgtgaaa tgccttgacg agaatatcgc gcaatttctt agtgacctcg
     4141 tcttcggtcc agaaaaaatt ctgcaagtcc tgcacccatt cgaaatagga cacgacaact
     4201 ccaccggcat tagcaagaat atcgggaata acaaaaattc ctttatcacg taaaatcgcg
     4261 tcggcttccg gcgtggtcgg accgttagcg ccctcggcta acactcggca ctgaatgcgt
     4321 gcagcattgt ttttcgtgat ctgcatttcc atagccgccg gcaccaagat gtcgcatttc
     4381 agctccagca gttccgcatt ggtgattttg tcgccatacg ggcagccttc gagactatga
     4441 ttcttgtccg cgacctcgag taattggtca accggcaaac ccttcgtatc gacaaagccg
     4501 ccggtgcgat cggcaatgcc gatcactttg acaccgatac cggccagctc tttggccgcc
     4561 accgaaccga cattgccaaa cccctggacc accgccgtgc atttcgcgag atcgatgttt
     4621 aagtgcttgg ccgcctcgat gatcgtgtag actacgccgc gacccgtcgc ttcgcggcgg
     4681 ccgacggtgc cgccgatggc aaccggtttg ccggtgacaa tctccggcac ggcatagcct
     4741 ttgtgctggg agtaagtatc catgatccac gccataacct gctcattggt gcccatgtcg
     4801 ggcgccggca cgtctacttc aggtccgatg aagttcagta actcggcggt gaagcgccgg
     4861 gtcatggatt gaagctcgtg acgcgacagc tcagccggat cgcaagcgat gccgcccttg
     4921 gcgccgccca gcggcaaacc aaccagcgaa catttccagg tcatccacat cgcaagcgcc
     4981 gacacctcgc cgaggttaac cgccggatga tagcgaatgc cgcctttaaa cggtcccagc
     5041 acatcgttgt gctgcacgcg atagccggta aagacatgga cccggccgtc atccatacgc
     5101 gtcggcacac tgacgatcat cgcacgatct ggccggcgca aacggtttgc taaatttggg
     5161 tcgacaccga gtttgctggc agcgacatcg aactgctgta ccgccatctc gttagtttca
     5221 ctgcgccatt ccagagtcgc cataatcctc ccgctcgcta ggttgacgaa acaccggctc
     5281 tagcccggcg gccaggtcat ctgccggccg cccagaaggt ggtagtgaag gtgaaaaacg
     5341 gtttgtccgg catcgcgacc cgagttgact accaggcgaa aacccctatc ggcaatcccc
     5401 tgctcgcgcg ccaatcggcc ggcgacctca aagatccgtc cgatcacgcc gctatcgacc
     5461 gcctcgatgt cgagcacgct ggcaatgtgc ttacgcggta tgatgagaat atgcacaggt
     5521 gccttgggcg cgatgtccgt gaacgccagc accgcatcgt cttgataaat caagttagct
     5581 ttgatcgtgc cgtcggcaat gccgcaaaat aggcagtcgg ccatggacat ctccttgcat
     5641 gcagttgtga aggggatttt tactcgtcgg cggcgccggg cagcttggca tagatctgcc
     5701 cgtcgacggt ctcgattggc aggctttgca gcgagagccc gacgcagggg ccccaaatgc
     5761 attcgccggt aggcggctca aagaccgcgc cgtgggtagc gcacataagg taacgattgt
     5821 cagagctgaa gaactgattg ttaacccagt cgagggtgat gccggtatgg ggacatcgat
     5881 tgatataggc gaagtgtgtg ccgtcgaaat tgacgagcaa cgcttcaaac tcgcgcccgg
     5941 cacgcttcat ggtaaatttt ttgctcttgc catgcggcaa ttcgccgact gtgccgacca
     6001 atttgcctga tggtttcttc gctgcagtca tagcagatct tttatcctat tctcccatgg
     6061 gcctcaaatc gccctccggg cagcccacca acacctcacc gcgatcgatc tgtaccggca
     6121 aacgatagag cgactcgccc ttgcatggcc ccgagacaca taacccgctg gcgaactcat
     6181 agagagcacc atgggtgtag cattgcaggt accggccgtc gtcgctaaaa aattcatcgc
     6241 gaacaaaatc caacggcgtc ggcatatggc ggcaacgatt cacataggcg tagtagttac
     6301 cctgatcgtt gataagaatc gcgtcaagcc ggtactgctg gcaaattagc cagaacttct
     6361 tgaccgcgcc cggctgcacc tcgttgaccc gcgcaaccac ccggccgccg gtgcggctct
     6421 ccgccgccat gcggttgcga tcgcgcacga agatcctccg ccggaccatc aaaccgatca
     6481 aataaagcac aagaaaaata agcgagagat agatcgtcag cgccatgagc ggatcgcgca
     6541 gagagctaat cggattgctt gttgacgctg tttcgaatgc gcttgacctc aggcgcagtt
     6601 tggcggccag agattcccat caccttcatc aactcgctgg tcatttcagc agtcacctga
     6661 tcgcgtacgc tacccttgcc atcgggatca gcgtcgagaa attttagaat cttgtcctta
     6721 gcgacgccgg tttccgccag accgtcaagg aactggacaa atcccacttt gccgcgcaac
     6781 tcctcgactc gcgccaaaag taatttgcaa tcggcgcaga ctctcccgaa aaacccggcg
     6841 cgctcgccac aataaatgca ttgccgcata atggttcttt ggccgattaa cagactattt
     6901 ggcaccgttc atcgccgctg cccacagacc ttgcagtccc ggtgtcagcg cagcgctcgc
     6961 tgcgaccacg ccgcgatgct tggctggctg tcgattaaac ctcatggcgt ggccatcgcc
     7021 gtcaaccacg gcgccgcccg ctgcctcgac cattaggaca ccagcgcaga tgtcccattc
     7081 gtgaatcgat cggaaagtca gggtgccgtc accatcgccg ccggccactt tggccaggcg
     7141 ataggcgatg ctccccaccg gctcgatagc gcagcggtcg acgaacactt gaaacctttt
     7201 cgccggttcg gagcgactga ccaatagccg cgcgccgtcg atctgttcgc ggctcgtgac
     7261 gtggatagag ttgtcgttca agaaagcgcc atgcccggcc gccccagtga aaaattcccc
     7321 ctgcgccggg ttatacacca cggccgcctg cgccacaccg tcgacgacga agccgatgga
     7381 gatcgcaaac tgcggcactc cttcgatgaa ctccttggta ccgtcgatgg gatcgactac
     7441 ccagacgcgc ggcaacgtca aacgccgctc gctatccgca tcctcctccg ataaccaacc
     7501 gtcatcggga aaagcatcag cgatgcgctc acggatgatc cggttggctt cgagatctgc
     7561 cgtcgtcacc ggattgtttt tacttttctc gcggacatcg tatttgccgc gaaacaggcc
     7621 catgatcgcg gcgccggcat cgcgcgccgc ctgctcggcg atgccgagct cttcgctgaa
     7681 ttgaaaatcg atggtcacat ttatcctttc gctgatagcg gactggttaa ttaaacacga
     7741 caacctgagg cgcagcaaat ggctcggtaa acagtcagca aggcaactct tcaacgccgt
     7801 ggattattcg cgcaccggca agagacattt cctccagcgc ccgagccgaa tcgccaggga
     7861 ccaaatcaac tccgcgaatc gcatccaaaa gaaccgtcgc gtcaaaacct tgctgcaaac
     7921 cgtcgagcac cgtctgtctt acacagtagt cggtcgcaag gccgccgaca aagatgtgct
     7981 caactccgcg ggcacgcagc aaaaccgcta aatcgttacc gtgttcatcc aacgcgtcaa
     8041 agccggaata gctatcggca tcggccattg tccctttcga aagaacggtc gcagtcgaac
     8101 ttagtcgcag agccgaatga aactcggccc ccagggtatt ctgcacgcag tgcaccggcc
     8161 agacgcctcc gta
//
LOCUS       DATLCH010000314        14458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10122783, whole genome shotgun sequence.
ACCESSION   DATLCH010000314 DATLCH010000000
VERSION     DATLCH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 14458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14458
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10122783"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..892)
                     /locus_tag="VMZ02_09310"
     CDS             complement(116..892)
                     /locus_tag="VMZ02_09310"
                     /inference="COORDINATES: protein motif:HMM:NF004868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HUR72173.1"
                     /translation="MADTPKLVSAMGRAELDKIYVRNYDLNKDLLGKISFSQMVCLML
                     QGRLPTADEGKMIDSMLIVLVDHGMTTGAAAARMTFHSAPEAIQGAVAAAILGAGSVH
                     LGSSEYCAKMLSDALAKEAEDADLDAVALKTVERRLANNQLIPGIGHGIHTGGDPRAD
                     RLFEIAQETKVYKRNCELLKKIAKIADQKVGKHLPVNVTGAIAAISQDMGFHWQMSKS
                     FAILGRALGGLAHVGEEIRRPIARGISNLIRDSLQYEPEK"
     gene            complement(967..2067)
                     /locus_tag="VMZ02_09315"
     CDS             complement(967..2067)
                     /locus_tag="VMZ02_09315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72174.1"
                     /translation="MADNEIRIIDIDGHVREADDLFEKYLEAPYRERAPKVEKVANGQ
                     LLFRLEGDRHHRRPDESPFRIKTDGSPVNEGRHLATHPKQRLEAMDRDRIERGLLFPS
                     AGLYLTSVHEEGYAAALCRAYNNWLYDYCGADRKRLMGVAALPMQDVKLAIDEAKRAV
                     NELGFKGVFVRPNPVKGRNIDDPYYDPLYKAVVDLGVPLLIHEGSGAFMPTAGADRFT
                     GQWFFTHTISHPLEQMLASLSLICRGALERHPELQCVFLEAGAGWLPYWLWRMDEHYE
                     VLPFQVPWLKLKPSDYFRRQCFVSFEADETRLGEVIASIGADRVVFASDYPHWDATFP
                     GVTDMIINRKDLDGDTQRKIMGGNAAKLLKLN"
     gene            complement(2350..3294)
                     /locus_tag="VMZ02_09320"
     CDS             complement(2350..3294)
                     /locus_tag="VMZ02_09320"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72175.1"
                     /translation="MNFVAKSFAALLIVSAAWQLGVAAERPPEKIRIAVSSKSLGFLD
                     TWAAKERGFFRKHSLDAEIIAMRPPLTIGALQAGEIDYAFGASTISRGAISGAPVRIV
                     SLALRSSFHTLVSRPAIKTITDMKGKTVAVTVGAADDFVARHLLRRGGVDARDVHFVN
                     MGGSDTRFPALHTGTIDASPLSLPFFVVAKRQGFNLLGTASDVLEMATVGIGTSIKKI
                     SQERDQVKKMVRAQLDTLRWIKTQKAEVVPFLQKFYSLDEGMAVESHAIYAKLIIDDA
                     RPLAEAIKTVLDQQGKADLPLDRVVDASVVEEVLRERR"
     gene            3378..4361
                     /locus_tag="VMZ02_09325"
     CDS             3378..4361
                     /locus_tag="VMZ02_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72176.1"
                     /translation="MAFRQVFMTKISLLALVLVCWLCPKRANAADKLRVSNCYIGGAI
                     LPLWMAKDAGFYQREGLEVEQVTIQGNPAVASLVAGEIDLLYCIPHNVISAMAGGAEL
                     QFIASVYNRMQYRIVAAPGIERVEQLKGKLIGVARIHDVAHFYVRLALKKFNLNADQD
                     VRFLATGGQAERTLALKNGRVQASILNPAYALTLEKDGFKTILDLESLNFPVLGNMTS
                     TRRALLKEKRPLAVRFVRALAASMKKIQDDPELSKKILAKNLRLQDKAVIEENYRFNS
                     GRYLEGFPTLPMDGLRYAIDSLVPTVPAVKNLKPEALVDPSILAEVAKSMP"
     gene            complement(4633..5616)
                     /locus_tag="VMZ02_09330"
     CDS             complement(4633..5616)
                     /locus_tag="VMZ02_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72177.1"
                     /translation="MKTFFVGLASVTVISLCPSWLLAAERITAAYTSIAAAYAPFWVA
                     KDKGIFDKYNFDARLIYMRGTVPTLAALANSELDFVQSGASPFIPFAAKGGDVVLLGC
                     LANKVIDYVLVAHPSITRIEQLRGKPIGISRVGDQTHHYIREILKRSGISLKEVRLVQ
                     TGLQPERVAALRQGLTLASILNRPNNLPLEQEGYKRLLDIEDLKLPAGVRCLITTRKI
                     MKAKPAMVDNFVMAWLESVRYILTQKRETKQVIAKYTLNTDDVHLEEAWNTLATQTEI
                     PPYASVSQLQGQANMMAEDQPDLTRFDAKVMVDNSVIKKLEDSGWTKKLFR"
     gene            complement(5851..6825)
                     /locus_tag="VMZ02_09335"
     CDS             complement(5851..6825)
                     /locus_tag="VMZ02_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HUR72178.1"
                     /translation="MSKPQPRIVILDAATYGDIPLTPFTGTWDCTVHQVTHAEQTRAR
                     LAGHTIAITNKVVITESVLISPDARGLKLIAVAATGTDIIDKEAAARRGVTVCNVPGY
                     ASQSVAQFTMALILELASRASSYAAAVKAGEWQKSPVFTLLSFPNVELHGKKLGIIGY
                     GNIGRTVAQMARGFGMEILIGVRPGSPTPSPDRVPLTELFRQADFVSLHCPLTPETRN
                     LVNQESLALMKRSAFLVNTARGALIDEPALVEALRTNVIAGAALDVVTQEPPPADHPI
                     IVAAKNLDNLIVTPHTAWSAREARQRLIEEVRENVAAFLQGRERNRVA"
     gene            complement(6822..7637)
                     /gene="mazG"
                     /locus_tag="VMZ02_09340"
     CDS             complement(6822..7637)
                     /gene="mazG"
                     /locus_tag="VMZ02_09340"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HUR72179.1"
                     /translation="MSDRETEEKFTELLKVMAQLRGESGCPWDKEQTHLSLKPCMLEE
                     TYELLDALDDGSANKLKEELGDVLLQVIFHSQIAREENRFTIKDVIDTLTAKLIRRHP
                     YVFAGEALPQDPAAALKQRAQLKANEKTDGESKSALGNVPKAMPALARAQSISRRAAS
                     LGFEWPNIEQVWAKVEEEMRELKEAVTAGDKVHTGEELGDLLFTMVNIARFLDVESED
                     VLARTIDRFMRRFQHIEAKLRQVNRGFDQSSIEEMDRYWDEAKQLEAQGKLAK"
     gene            7749..8597
                     /locus_tag="VMZ02_09345"
     CDS             7749..8597
                     /locus_tag="VMZ02_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HUR72180.1"
                     /translation="MPVVNVDGIDTYYELHGSGTPILMCAPGGFDATLDKWRVASAWT
                     GIDAIQALAAEHTVILYDRRECGRSGGRLERLSWKVLADHGKALLDHLQIDAAWVMGG
                     CMGCSVALAFGVHFPQAARGLLLHWPVGGFRWKTNSQARFLRHYEYVKQNGLKGVVER
                     AQEKKNFWLDPEAGPWAGLIAGDKAFAERFAQQDVEHYLGIIITSGRSLFDRDTATGA
                     EPEEVMGIKAPSLVIPGDDQSHATSGAHYLRELLPNNEFWNVMPPGQTTQTVVDRILQ
                     FCREHG"
     gene            8695..9414
                     /locus_tag="VMZ02_09350"
     CDS             8695..9414
                     /locus_tag="VMZ02_09350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR72181.1"
                     /translation="MDVSSSTVQLNTSDGKMDAYVAQPKDSGTYPGIVVIQEAFGVND
                     HMKKVTERIAAEGYVAICPDIFHREAERVIPFSDMPKAIATLQRVQDPKAMEDVGAAL
                     AHLKSQSNVKGGSLGVVGFCMGGRLTYLAAAHHANDVKCAVAFYGGGITMGNPSPLSR
                     TGEIKCPMYLFFGAKDQLIPMDQVGQINTELTTHKVPFQMKIYPDAGHGFFCDERGSF
                     NEKAAQDAWEKTKSFFAQHLK"
     gene            9510..10394
                     /locus_tag="VMZ02_09355"
     CDS             9510..10394
                     /locus_tag="VMZ02_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HUR72182.1"
                     /translation="MAKTKKSDQIKVALVQMSCANDPSDNIAKAVQRIEAAAKSGAKV
                     VCLQELFRSRYFCQSEDHDNFTLAEAIPGPSTETLSAVARRKQIVIVASLFERRTAGI
                     YHNTAVVLDADGSIAGKYRKMHIPDDPLYYEKFYFTPGDLGFPSFQTRYAKVAALVCW
                     DQWFPEGARAAALGGAQILFYPTAIGWIPNEPRTVAQNQRNAWELIQRSHAIANGIFV
                     AAANRVGSEGKIKFWGNSFVAGPLGEIVARAGDKREEILLANCDLNKIEETRQSWPFL
                     RDRRIDAYGALATRSLDR"
     gene            10420..11484
                     /locus_tag="VMZ02_09360"
     CDS             10420..11484
                     /locus_tag="VMZ02_09360"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HUR72183.1"
                     /translation="MAEPFPSSFTPSQLGYSMPAEWAPHRATWLSWPHNLETWPDNLE
                     KVRDIWIQMVTALSPHEQVCLLVNDARVKTDVLERLNRAKAAMANVTLLEIPTVDVWM
                     RDYGPTFITRNAADRPLAFNDWIFNGWGGKYKTYEEDERVARAIARLLQVPVFEHKLV
                     LEGGSIDVNGAGTCLTTEQCLLNRNRNPHLSRAQLEEFLKESLGARQILWLGEGIVGD
                     DTDGHIDDIARFVDPRTLVCILESNPRDENYRVLQENYERLQSARDQDGSKLEILCLP
                     VPTPLFYDGARLPASYANFYIANDVVLVPVFDDAKDQIALGLLQECFPTRKVVGLRCN
                     EVVAGLGAIHCVTQQEPALR"
     gene            11560..11772
                     /locus_tag="VMZ02_09365"
     CDS             11560..11772
                     /locus_tag="VMZ02_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72184.1"
                     /translation="MGQVIQLQELHQARRRKSEKVSMHQCIDLLEWNLKKSVDDYFTA
                     PREERSMRATQIRKLSEILEYALRLL"
     gene            11790..12917
                     /locus_tag="VMZ02_09370"
     CDS             11790..12917
                     /locus_tag="VMZ02_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HUR72185.1"
                     /translation="MDFAPVFVTVFISLVGVIVLGWFFLGRLRLLLSEKPEDQSLLLM
                     QQQIDQLRSQLSSTLDNNTRILQQQLGQMIGQVNERLKENAQVLNDTQQSLGERLDNA
                     ARVVGGVQKSLGGLEEANRRIYEVGKDIASLQEILRAPKLRGGLGEFFLGDLLGQILP
                     PQHFSLQHGFRSGEKVDAVISLGASLVPVDAKFPLENFKRLLEAASDDERNRAKKQFA
                     ADVRKHIDAIASKYILPDEGTYDFALMYIPAENVYYEAVIKDEAADERNLCQYAMSKR
                     VIPVSPNSFYAYLQAIILGLKGLRLDDRTKEIIQYLGRLQGDFTRFRDDFVVLGKHLA
                     HAQSSYQSADKRLEQFSQKFLAAESAQSELVDSRNAGVKSD"
     gene            12927..14369
                     /locus_tag="VMZ02_09375"
     CDS             12927..14369
                     /locus_tag="VMZ02_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HUR72186.1"
                     /translation="MHLTLFSRLTLGYLAIFAIVAAASSYAIFELTDFGNQAESILRI
                     DNRVLEHEKLLTDLLLAQSRAEQKFVLSRDEAWYLQFVRLKIDFEARAESAAAVADVL
                     AQSIVRRVRENYRRYVDLVELEARRVRTRKDYPQARFKQQKDNLVDAMLDELERLRVN
                     QQLTTYSKVKELAAAAAQARQAALSIASGCLLAIILMSLLLTKSITRPLAALKAKTQE
                     IASGNFDHPVDIRSPREIGELAVALNSMSQRLKELDYIKADFFASMSHELRTPLTSIK
                     EGTGLLLDGVGGATTEKQQKLLGIITEESNRLISLVNTFLDLSKMEAGMMHYDFESTH
                     IEPLIKRAVAEITPLVEAKQIQFESALNEPMPAVRIDPERILQVLRNLMGNAVKFTPK
                     GGRVSVAAKTHDGILEISVKDSGPGIPAESLRSIFEKFSQGSHASGTNRQGTGLGLAI
                     AKNIVTSHGGNIWAESQLGGGSKFVFVLPC"
BASE COUNT         3134 a         3906 c         4167 g         3251 t
ORIGIN      
        1 tgcaagacta agaatcaata cgaccgtgaa cgcaatatcg ccgctcggcg cggttgtttt
       61 cattcgtccc atcactccaa cactccagta aaaaagatgt ccattcgaag ggaacttatt
      121 tctccggctc atactgcaga ctgtcgcgga tcagattcga tatgccgcgg gcgatgggcc
      181 ggcgaatttc ttcgccgacg tgggcgagac cgcccagggc acggcccagg atcgcgaagc
      241 ttttcgacat ctgccaatgg aagcccatgt cctgagagat tgcggcaatg gcgccggtga
      301 cattgaccgg cagatgtttg ccgactttct ggtcggcgat cttggcgatt tttttcaaca
      361 gctcgcagtt gcgtttgtag accttggtct cctgggctat ctcgaacagc cggtcggcgc
      421 gcgggtcgcc gccagtatga atgccatggc cgatgccggg gatgagttga ttattcgcta
      481 gacggcgttc gacggtcttg agcgcgaccg cgtcgaggtc ggcatcctct gcttctttgg
      541 cgagcgcatc gctgagcatt ttcgcgcaat actccgacga gcccagatgc acgctgccgg
      601 cgccgaggat cgccgcagct acggcgccct gaatcgcttc gggcgccgag tgaaaagtca
      661 tgcgcgcggc agcggcgccg gtggtcatgc catggtcgac cagcacgatc aacatcgagt
      721 cgatcatctt gccttcatcg gcagtgggaa gccgcccttg gagcatcaag cagaccatct
      781 gcgagaaact gatcttgccg agcaggtcct tattcaaatc gtagttgcgc acgtaaatct
      841 tatcgagctc ggcgcggccc attgcggaaa caagtttcgg tgtgtctgcc atgctatcct
      901 ccaggcctta attttgcgtt tccagtttgg gttgcgatcg aaaactttag accggaaacg
      961 tcgtagttaa ttcagcttca acaactttgc ggcattgccg cccataattt tacgctgcgt
     1021 gtcgccatcg agatctttgc gattgatgat catatcggtg acaccgggaa aagttgcatc
     1081 ccagtgcgga tagtcggagg cgaacacaac acggtcggcg ccgatggatg cgatcacttc
     1141 gcccaagcgg gtttcgtcgg cttcgaatga gacgaaacat tggcggcgga agtagtcgct
     1201 cggtttcagt ttcagccacg gcacttggaa cggcagcact tcatagtgct cgtccatgcg
     1261 ccagagccaa tagggcagcc agccggcgcc cgcttcgaga aaaacgcact gcaattcggg
     1321 atggcgctcc aaggcgccgc ggcagatcaa gctcaagctc gcaagcatct gctccaaagg
     1381 atgagaaatt gtatgagtga aaaaccactg accggtaaaa cgatcggcgc cggccgtggg
     1441 catgaacgcg cccgaaccct catggatcaa cagtggcacg ccgagatcga cgactgcctt
     1501 atagagagga tcataatacg gatcgtcgat gttgcggcct ttgaccggat tcggtcggac
     1561 gaagacgcct ttgaagccca actcattgac cgcgcgtttc gcttcatcga tggcgagctt
     1621 gacatcttgc atgggcagcg cggcgacacc catcaagcgc tttcgatctg cgccgcagta
     1681 gtcgtagagc caattattgt acgcgcggca aagcgcagcg gcgtagccct cttcatgcac
     1741 cgaggttaag tacaaaccgg cggaggggaa caataggccg cgctcaatgc gatcgcggtc
     1801 cattgcctcg aggcgctgtt tgggatgggt cgccaagtga cgtccttcat tcaccggtga
     1861 accgtcggtc ttaattcgga atggcgactc gtcggggcgg cgatgatggc gatcgccctc
     1921 cagtcggaat aacagctggc cgttggcaac tttttcgacc ttgggagcgc gctcgcgata
     1981 cggcgcctcc agatacttct caaacaagtc gtcggcttcg cgcacatggc cgtcgatgtc
     2041 gatgatacga atttcattat cagccatttt tctggtcctt cgaagaggta ttcaccacga
     2101 acgagacgaa gagcacgaac tcccgaagga atatgtgcca aaaccgtcgt atccttcgtg
     2161 ccgcctttga aaatacagct aaagaaagat ctttcacttc gttcgagatg acagaaatgc
     2221 ttgctttgtt gtcattgcga ggcccgcgag aaatccttct ccagtctttg catttcctta
     2281 taggtcatcg tggtgaacga tttgaattta acatacatca tcgaccgcaa tgcccgtaaa
     2341 gctcctattt catcgccgtt cgcgcaatac ttcctcgacc accgaagcgt cgaccacacg
     2401 atcgagcggc aaatccgctt tgccctgctg gtcgagcacc gtcttgattg cctccgccag
     2461 cggcctggca tcgtcgatga tcaatttggc atagatcgcg tgggattcga cagccatgcc
     2521 ctcgtcaagg ctataaaatt tttgcaggaa cggcacgact tccgccttct gtgtcttgat
     2581 ccagcgcaga gtgtcgagct gcgcgcgcac catcttttta acctggtcgc gttcctgcga
     2641 gatttttttg atcgacgtgc cgatgccgac tgtcgccatc tcgagtacat ccgatgcggt
     2701 gccgagcagg ttaaaaccct ggcgcttggc gacaacgaag aacggcaggg aaagtggcga
     2761 ggcatcgatg gtgccggtgt gcagcgcggg aaagcgcgtg tcggacccgc ccatgttaac
     2821 gaaatgaaca tcgcgcgcat cgacgccgcc gcggcgcaat agatggcgcg cgacgaagtc
     2881 gtcggccgcg ccgacggtaa ccgcgacggt tttgcccttc atgtcggtga tggtcttgat
     2941 ggccggtcgg gatactaagg tatgaaacga cgatcgtaag gcgaggctga cgatgcgcac
     3001 cggcgcgccc gaaatggcgc cgcgcgagat tgtgctggcg ccaaaggcat aatcgatctc
     3061 gccggcttgc agtgcgccga tggtgagtgg cgggcgcatg gcgatgatct ccgcgtcgag
     3121 actgtgtttg cggaaaaatc cgcgctcctt tgccgcccag gtatcgagaa atcccagcga
     3181 cttcgaagat accgcgatgc gaatcttttc cggcgggcgt tcggcggcga cgccgagttg
     3241 ccaagcggcg ctcacgatca gcagcgccgc aaagctttta gcaacaaagt tcattatgga
     3301 tcttctgtaa cgatgcagcc gcaagatagc aagggatgct tgctacgaca ataactccac
     3361 gttagggtcg aaaccctatg gcgttccgac aagtgtttat gacgaagatt tcgctgttgg
     3421 cattggtatt ggtctgttgg ctttgcccga aaagggcgaa cgcggcggat aagttgcgcg
     3481 tcagcaattg ctacatcggc ggtgccatct tgcccttgtg gatggccaag gacgcgggct
     3541 tctaccagcg tgaaggactg gaggtcgagc aggtaacgat ccaaggcaac cccgcagtgg
     3601 catcgctagt ggccggcgag atcgatctcc tgtattgcat accccacaat gtcatcagcg
     3661 ccatggcggg cggcgccgag ttgcaattca tcgccagcgt ctacaaccgc atgcagtatc
     3721 gtatcgtcgc tgcgccgggc atcgagcgcg tcgaacaatt gaaaggcaag ttgattggcg
     3781 tggcgcggat tcacgacgta gcgcattttt atgtgcgttt ggcactgaaa aaattcaact
     3841 tgaacgccga tcaggacgtg cgtttcctcg ccaccggcgg tcaggctgag cggacgctgg
     3901 cgctgaaaaa tggccgcgtg caagcttcga tccttaaccc cgcctacgcc ctcacgttgg
     3961 aaaaagacgg attcaaaacc atcctcgatc tcgagtcgct caatttcccg gtgcttggca
     4021 atatgacctc gacgcggcgc gccttattga aggaaaaacg gccgctcgcg gtgcgcttcg
     4081 tccgtgctct ggcggcaagc atgaaaaaga ttcaagacga cccggagttg agcaagaaga
     4141 tcttggcaaa aaatctgcgc ctgcaagaca aagctgtcat cgaggaaaac tatcgcttca
     4201 attctggaag atacttggaa gggtttccga cgctgccgat ggatgggctg cgctatgcca
     4261 tcgattccct ggtgccgacg gtgccggcag tgaagaactt gaagccggaa gcgctggtgg
     4321 acccgagcat tctggccgaa gtagcgaaat cgatgccgta ggtgaaaaag aattcttcac
     4381 cacgaaagac acgaacggca cgaacttcgt aggaatattt ttcaagacct actatcttcg
     4441 tgtctcttcg agaatagata tgcgttacga attcgcagga gaacccgtca taccggcgaa
     4501 cgccggtatg catccctttc ttatcctgga tgccggcctg cgccggcatg acgaatcgag
     4561 gggaattata atactccgta gaacagctta agcccatgga catctcaccg tgagcctctt
     4621 atctcctgat tcttagcgaa ataacttctt ggtccagccc gagtcttcca actttttgat
     4681 gacgctatta tccaccataa ctttggcatc gaagcgggtc agatcgggct gatcttcggc
     4741 catcatgttt gcttggcctt gcagttggct gaccgaagcg tagggcggga tctcggtctg
     4801 ggtcgctagc gtattccatg cttcttcaag gtggacgtcg tcggtgttga gcgtgtactt
     4861 ggcgatgacc tgtttggtct cgcgtttctg cgtcaggatg tagcgcaccg actcgagcca
     4921 agccatgacg aagttatcga ccatagccgg tttggctttc atgatcttcc gcgtggtgat
     4981 caggcaacgc acgccggcgg gcaatttgag gtcttcgatg tcgagcaaac gcttatagcc
     5041 ttcttgctcc agcggcagat tgttagggcg gtttaggatg cttgccaagg tcaagccttg
     5101 gcgcagcgct gcgacgcgct cgggctgcag tccggtctgg accaagcgca cctccttgag
     5161 agagatgccg cttcgcttga ggatttcccg aatataatga tgagtctggt cgccgacccg
     5221 gctaatgccg atgggcttgc cgcgcagttg ttcgattctc gtgatcgatg gatgggcaac
     5281 aagcacatag tcgattactt tattagcgag gcaaccgagc agcaccacat caccgccctt
     5341 ggccgcgaaa ggaatgaacg gagaggcgcc ggattgcacg aagtcgagtt cgctgttggc
     5401 cagcgccgcg agcgtcggca cggtgccgcg catatagatc agccgagcat cgaagttgta
     5461 cttatcaaag atgcctttgt ctttggcaac ccagaatggc gcgtaggccg ctgcgatgct
     5521 ggtgtacgcc gcggtgatac gttccgcggc taagagccac gacgggcaca ggctgataac
     5581 ggtgacacta gccagaccaa caaaaaaagt tttcatcgcc ggcattacag cctattcgac
     5641 atagtgaagc aagtttgccg gagcgaacga aggctgagct gttccggttg aatcgcgccg
     5701 gtcagcagga acgaaaacca tttcggcgag ccggcctcgg cgcaactcag agttgagttg
     5761 tcgtgaatgc gatgggtcga atgctgtcga agaatcagcg ggatgaaatc accgctagaa
     5821 gacgagggcg cttggttaat tcaccgacac ttaggcgacg cggtttcgct cccggccttg
     5881 taaaaatgcc gcgacatttt ctctgacttc ctcgattagc cgctgacgcg cttcgcgggc
     5941 cgaccaggcc gtgtgcggcg taacgatcaa gttgtccaag ttttttgccg cgacaatgat
     6001 cgggtggtcg gcgggcggcg gctcttgcgt gacgacgtcg agtgccgcgc cggcgatcac
     6061 gttggtgcgc agtgcttcaa ctagtgccgg ctcatcgatc agcgcaccgc gcgcggtatt
     6121 tacgagaaag gcgctgcgct tcatcaatgc caaagactct tgatttacaa ggtttctcgt
     6181 ttccggtgtc agtggacagt gcagcgagac gaaatcggct tgacgaaata gctccgtcag
     6241 cggcacgcga tcgggagatg gtgttggact tccggggcgt acgccgatca ggatctccat
     6301 gccgaagccg cgggccattt gcgcgaccgt cctgccgata ttgccgtagc cgatgatacc
     6361 gagtttttta ccgtgaagct cgacgttcgg aaagctcagc agtgtaaaga cagggctctt
     6421 ctgccactcg ccggctttga ccgcggccgc atagctgctg gcgcgactgg ccagctccag
     6481 gatcagcgcc atggtaaact gcgccaccga ctgactggcg tagcccggca cattgcagac
     6541 cgttacgccg cgtcgggctg cagcttcttt gtcgataatg tcggtgccgg tcgcggcgac
     6601 ggcgatgagt ttcaggccgc gagcgtcagg cgaaatcaaa acgctttcgg tgatcacgac
     6661 tttattggtg atggcgatgg tgtgaccggc gaggcgggcc ctggtttgct cggcgtgggt
     6721 gacttggtgc accgtgcagt cccaagtgcc ggtgaatggc gttagcggta tatcgccgta
     6781 ggtggcggcg tcgaggatga ctattctagg ttgcggcttg ctcatttcgc cagcttgcct
     6841 tgagcttcaa gctgtttcgc ttcgtcccag tagcggtcca tttcctcgat ggaggattgg
     6901 tcgaatcctc tgttcacttg gcgcagcttg gcttcaatat gctgaaagcg gcgcataaag
     6961 cggtcgatgg ttcgggccag aacatcctcg gactcgacgt cgagaaagcg cgcgatgttg
     7021 accatggtaa aaagcaagtc gccaagctcc tcgccggtgt ggactttatc gccggcggtg
     7081 acggcttctt tgagctcgcg catttcttcc tcgaccttcg cccaaacttg ttcgatattt
     7141 ggccactcaa agcccaaact cgcggcgcgc cgcgagattg attgcgcccg cgccagcgcc
     7201 ggcatcgctt tgggaacatt acccaatgcc gatttgcttt cgccgtctgt tttttcgttg
     7261 gctttgagtt gcgcccgctg tttgagcgcg gcggcgggat cttgcggtag cgcttcaccg
     7321 gcgaagacgt agggatggcg gcgaatcaat tttgcggtga gtgtgtcaat cacgtctttg
     7381 atcgtgaacc ggttttcttc tctggcaatc tgtgaatgaa aaattacctg cagcaggacg
     7441 tcgcccagct cttctttcag tttgttggca ctgccgtcat cgagcgcgtc gagcagttca
     7501 taagtttctt cgagcatgca gggcttgagg gagagatgag tctgctcttt atcccaggga
     7561 cagccgctct cgccgcgtag ctgcgccatc accttaagaa gttcagtaaa tttctcttca
     7621 gtctcacgat cactcacaga tctgtcctcc gcgctgcttt gatttgggaa ctttctttcc
     7681 gggcagacag cgtgctaaat cttactgatc gtctccgttt aagtccagca aaccgaggag
     7741 agctgcttat gccggttgtc aatgtcgatg ggattgacac ttactacgaa cttcatggtt
     7801 cgggaacgcc gatattaatg tgcgcgccgg gcggattcga cgcgaccctc gacaaatggc
     7861 gggtcgccag cgcttggacg ggaatcgacg cgatccaagc actagcggct gagcatacgg
     7921 tgattctcta tgaccggcgc gaatgcggcc gctccggcgg gcggctcgaa cgtctgagct
     7981 ggaaggttct tgccgatcac ggcaaagcgt tactcgatca tttgcaaatc gatgcagcgt
     8041 gggtcatggg cggctgcatg ggttgttcgg tcgctctcgc tttcggcgtt cattttccgc
     8101 aagccgcacg gggactgctt ttgcattggc cggtgggcgg ctttcgctgg aagaccaaca
     8161 gtcaagcgcg atttctgcgc cattacgaat acgtcaaaca gaatggactc aagggcgtcg
     8221 tcgagcgcgc tcaggaaaag aaaaactttt ggctcgatcc cgaagccggt ccctgggcgg
     8281 gtctgatcgc gggcgataaa gcattcgccg agcgctttgc ccaacaagat gtggagcatt
     8341 acttaggcat catcatcacc agcgggcgta gtctattcga tcgcgatacc gcgaccggtg
     8401 ccgagccgga agaggtcatg ggaatcaagg cgccctcgct ggtgattcca ggcgacgatc
     8461 agtcccacgc tacctctgga gcgcattatc tacgcgagct cctaccgaat aatgaattct
     8521 ggaacgtcat gccgccgggg cagacaactc aaactgtggt cgatcgcata ctccaattct
     8581 gccgcgaaca cggttaaggt tgtcgagtgt gacgatggag gtgtggaccg tcatggcgaa
     8641 atttgataga caaaatccac ttcgaattgt cattcacgaa acaaggaggt agcgatggac
     8701 gtttctagtt ctacagtgca actcaacact tctgacggca agatggatgc ctatgtggcg
     8761 cagccaaagg atagcggaac ttatcctggg atcgttgtaa ttcaggaagc ctttggtgtg
     8821 aatgatcaca tgaagaaagt cacagagcgc atcgctgcgg aaggttatgt ggcgatctgt
     8881 ccggatattt ttcatcgcga ggcggagcgg gtaataccat tctccgacat gcccaaggcg
     8941 attgcgacgt tacagcgggt gcaagatccc aaggccatgg aagatgtcgg cgcggcgctc
     9001 gcgcacttga aatcgcaaag caatgtcaaa ggcggatcac ttggtgtggt cggcttttgc
     9061 atgggcgggc gcttgaccta tttggccgcg gcgcaccacg ccaatgacgt gaaatgcgcg
     9121 gtggcgtttt atggcggtgg cattactatg ggcaatccga gcccgttgtc gcgtaccggc
     9181 gagatcaaat gcccgatgta cctattcttt ggcgccaaag atcagttgat tcccatggat
     9241 caagtcggcc aaatcaatac cgagttgacg acccacaagg taccgttcca gatgaaaata
     9301 tatcccgacg ctgggcacgg ctttttctgc gacgagcgcg gcagcttcaa tgaaaaggcg
     9361 gcccaggacg cctgggaaaa gaccaagagt ttcttcgcgc agcatcttaa ataactcacc
     9421 ttggcgcgtc ccgagggaga ggaaaggtgt gtcacttttt ctctcccttt tttgttttcc
     9481 ggtgcgtttc cgttaggttg attcccacca tggcaaaaac caaaaaatcc gatcaaatta
     9541 aagttgccct ggtgcagatg agctgtgcca acgatccttc cgacaatatt gctaaagccg
     9601 tgcagcgaat cgaagccgcg gcaaaaagcg gcgccaaagt tgtctgcctg caagagctgt
     9661 ttcgctcgcg ctacttttgt cagagcgagg atcacgacaa ttttacactt gccgaagcga
     9721 ttcccgggcc aagcacggaa acgttaagcg ctgtggcgcg gcgcaagcaa attgtgattg
     9781 tcgcgtcgct tttcgagcgg cgcacggccg gcatctatca caacacggcg gtggtgctcg
     9841 atgccgacgg ttcgattgcc ggcaagtacc gcaagatgca tatacccgac gatccgcttt
     9901 attatgaaaa gttttacttc accccaggcg acttgggatt tccaagtttt cagacgcgct
     9961 acgctaaagt agcggctctg gtgtgctggg accagtggtt tcccgaagga gcgcgtgctg
    10021 ctgccctggg cggcgcgcag atactgtttt atccgactgc catcggttgg attcccaacg
    10081 agccgcgcac cgtggcgcag aatcaacgca acgcttggga gctgattcag cgctcgcatg
    10141 ccatcgccaa tggcattttt gtcgcggcgg ccaatcgcgt tggcagcgaa ggcaaaatca
    10201 agttttgggg caattcgttt gttgcgggtc cgctggggga aattgtcgcc cgcgccggag
    10261 ataagcgcga agagattctg ctggctaatt gcgatctcaa caaaatcgaa gagacgcgcc
    10321 agtcctggcc atttctgcgc gatcggcgca tcgacgcgta tggcgcccta gcaactcgct
    10381 cgctcgatcg ttaactctcc cgctgcgaaa tcaacattca tggcagaacc atttccgagt
    10441 tcgttcaccc catcccagtt aggctactcg atgccggccg agtgggcgcc gcatcgcgcc
    10501 acttggctat cctggccgca taacttggaa acctggccag acaacttgga aaaagttcgc
    10561 gacatttgga ttcaaatggt aacggctttg tcgcctcacg agcaggtctg tttgctagtc
    10621 aacgacgccc gggtgaaaac cgatgtgtta gagcgactca accgcgcaaa ggctgcgatg
    10681 gccaacgtga cgcttctgga gattcccacg gtcgatgtct ggatgcgcga ttatggacct
    10741 acctttatca ctcgaaacgc ggcggaccgg ccactggcgt tcaacgattg gatcttcaat
    10801 ggctggggcg gcaagtataa aacctacgaa gaagacgaac gggtcgcgcg cgcgatagcc
    10861 cgcttgttgc aggtcccggt gttcgagcac aagcttgttc tcgagggcgg ttccatcgac
    10921 gtcaacggcg cgggaacctg tctcaccacg gaacagtgtc tgctcaaccg caatcgcaat
    10981 ccacatctga gccgggcaca gctcgaagag tttctcaaag agtcgcttgg cgcgcgtcag
    11041 attctctggc tcggcgaagg gatcgtaggc gacgataccg acggccatat cgacgacatc
    11101 gcacgcttcg tcgacccaag gacgctcgtc tgtattctcg agtcgaaccc gcgcgacgag
    11161 aattaccggg ttttgcagga aaattacgag cggttgcaga gtgcccgaga tcaggacggc
    11221 agtaaattag aaattctttg cttaccggtt ccgacgccgt tattttacga tggtgcgcgg
    11281 ttgccggcga gctacgcgaa tttttacatc gccaacgacg ttgtactcgt gccggtgttc
    11341 gacgacgcca aggatcaaat cgccctggga cttttgcaag aatgttttcc aacgcgaaaa
    11401 gtcgtcggac tgcgctgcaa tgaagtggtc gcgggtctgg gcgcgattca ttgcgtcacg
    11461 caacaagagc cggcgctgcg ctgaatcata ctttgcgcgc gattctttct tcttgaaggt
    11521 cttgactatt tttcgcgcta ccctatcctg acggctggta tgggtcaggt cattcaactc
    11581 caggagctgc atcaagcgcg ccggcgcaag tcggaaaaag tcagcatgca ccaatgcatc
    11641 gatcttttgg aatggaattt aaaaaaaagc gtcgatgatt atttcaccgc tcctcgagaa
    11701 gaacgatcca tgcgcgcaac ccaaatccgc aaactcagcg aaatcctcga atacgcgctg
    11761 agactgcttt agccccgccc acgcccgtcg tggatttcgc tcctgtcttc gttacggttt
    11821 ttatttcatt ggtcggtgtg attgtactcg gctggttttt cctcggtcgt ttgcgcttgc
    11881 tgctttcgga gaaaccggag gatcagtctc tgctcttgat gcagcagcag atcgatcaac
    11941 tgcgctcgca actgagttcg actctcgata acaacactcg aatcttgcag cagcagttgg
    12001 gccagatgat cggtcaggtc aacgagcgcc tgaaagaaaa tgctcaagtc ttgaacgaca
    12061 cccaacagag cctaggcgaa cggctcgaca atgcggcgcg cgtggtcggc ggcgtgcaga
    12121 aaagtctcgg cggtctcgaa gaggcgaacc ggcgcattta cgaagtcggt aaagacattg
    12181 cgtccttgca agaaattctg cgggcaccga agctgcgcgg cggcctcggc gaattctttc
    12241 tcggcgattt gttgggacaa attttgccgc cgcagcattt ttcgctccaa cacggctttc
    12301 gcagtggcga aaaagtcgac gctgtcatta gcctcggcgc ttccctggtg ccggtggacg
    12361 ccaaatttcc cctggaaaat ttcaaacggc tactggaagc ggcgagcgac gatgagcgca
    12421 accgggcaaa gaaacaattc gccgccgacg tgcgtaagca cattgacgcg atcgcttcca
    12481 agtatattct tccagacgaa gggacctatg atttcgcgct catgtatatc ccggcggaaa
    12541 atgtttatta tgaggccgtc atcaaagacg aagccgctga cgaacgcaat ctctgtcagt
    12601 acgccatgag caaacgggtg atcccggtgt cgcccaatag tttctacgct tacttgcagg
    12661 cgattatctt ggggctcaaa ggtttgcgac ttgacgatcg aaccaaagaa attattcagt
    12721 acttgggccg gctgcagggc gatttcacca ggttccgcga cgattttgtc gtcctcggca
    12781 aacacctagc gcatgcccag tcgagctacc agagtgccga caagcgactt gaacaattca
    12841 gtcaaaaatt cttagctgcg gaatcggcgc agagcgagct tgtggattcg cgcaacgccg
    12901 gcgtcaaatc cgactaacgc gtcgccatgc atttgacttt gttttcccgc ttgacgcttg
    12961 gctatctggc gatctttgcg atcgttgcgg cggcgagttc gtacgcgatc ttcgagctga
    13021 ccgattttgg aaaccaggcc gagtccattc tgcgcatcga caaccgcgtg ctcgagcacg
    13081 agaaattact caccgatcta ttgttggccc agagccgcgc tgaacagaag tttgtcctta
    13141 gccgcgatga agcctggtat ttgcaatttg tccgattgaa gatcgatttc gaggcccgtg
    13201 ccgagagcgc ggccgcggtg gcggacgttc tcgcccaaag cattgtcagg cgtgtgcgtg
    13261 aaaattatcg ccggtatgtg gatctggtgg aattggaggc gcgccgcgtg cgcaccagga
    13321 aggattatcc gcaggcgcgg ttcaagcagc agaaggataa tctggtcgat gccatgttgg
    13381 atgagctgga gcgcttgcgc gtcaaccagc aactgacgac ttactctaaa gtcaaagagt
    13441 tggcggcggc ggcggcacaa gcgcggcagg cagcgttgtc gatcgcctcg ggctgtctgt
    13501 tggcgattat cctaatgtcg ctgttgctga caaagagcat tacccggccg ttagccgcgc
    13561 tcaaagccaa aacgcaagaa atagccagcg gcaatttcga tcatcccgtg gacattaggt
    13621 cgccgcgaga gatcggcgag ttggcggtgg cgctgaactc gatgagccag cggctcaagg
    13681 agcttgacta cataaaggcg gacttcttcg cctccatgtc tcatgagctg cgcacccctt
    13741 tgacgtcgat caaagaaggc accggtcttt tgctcgacgg tgtcggcggc gcgaccacgg
    13801 agaagcagca gaaactgctc ggcatcatca ccgaggaaag caatcgcttg atcagcttgg
    13861 tcaacacctt cctcgatctg tcgaaaatgg aagctggcat gatgcattac gatttcgagt
    13921 cgactcatat cgagcccttg atcaaacgcg cggttgccga aattaccccg ttggtggagg
    13981 ccaagcagat tcaattcgaa agcgcgctga acgaaccgat gccggcggta cggatcgatc
    14041 cagagaggat tttgcaagtg ctgcgcaatc ttatgggtaa tgccgtcaag ttcaccccca
    14101 agggaggtag agtttcggtc gcggcaaaga ctcacgatgg catcctcgaa atttctgtga
    14161 aagactccgg tccgggtatc ccggcggaga gtttacggtc gatcttcgaa aaattcagcc
    14221 aaggcagtca cgcgagcggg actaaccgcc agggcacagg tctcgggctg gcgatagcca
    14281 aaaacatcgt tacatcgcat ggtgggaata tatgggcgga aagccaattg ggcggtggca
    14341 gcaagtttgt atttgttttg ccctgctgat aatcgtttcc ggctgtggtc tttttcagga
    14401 ctcgcagcgg cgtcgcgagt tgcgcgacgc gttgaccgag ggccagcagc agctcgcc
//
LOCUS       DATLCH010000315         4685 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7881591, whole genome shotgun sequence.
ACCESSION   DATLCH010000315 DATLCH010000000
VERSION     DATLCH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7881591"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1123)
                     /locus_tag="VMZ02_09380"
     CDS             complement(128..1123)
                     /locus_tag="VMZ02_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72187.1"
                     /translation="MVIDIHNHIVAGEQLQTFQAGLINSAGFHRPRGVVVSEKHIQEA
                     KWHGHRHSEVLGEVGTDFAFISPRPYTMMHSAKPEKIVHWYCQAVNDALALQVKMEPN
                     KFRAIGGLPQNAGVPVTNSFEEIDRCINDLGFVGIMINPDPGEGDGMTPAMGNEYWYP
                     LYEKMVKMDIPGLVHGASCKNPRESFHGHFITEESIAILSLVDSNVFKDFPKLKLIIS
                     HGGGSVPYQVGRWRSQLRGPNDTENFDQRLKQLYFDTALYNIESLKLLFRICGPERCL
                     FGTERPGAGSKTDPRTGKWYDDVKPNIDSIPWLTTDDKAKIFDQNALQVFSRFKQ"
     gene            complement(1241..2557)
                     /locus_tag="VMZ02_09385"
     CDS             complement(1241..2557)
                     /locus_tag="VMZ02_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72188.1"
                     /translation="MESAAELAHRTAKPKIFYGWYIVAVGFLANVASSFALASTMSIF
                     LKPLTADLGISRGVFSLLRSGEGIISACIAPLVGTLVDRYGGRWLMVIGTTIVALGYF
                     LLTHVENFAQFAAIRLTLVSLGDSMMGYMVVNVIIAQWFVRQRSRALACSSMGVGFAK
                     VCMPVLAAWLILTLGWRQTWFVFGVMTLVLLVVPALLVIRRSPESMGLLPDGATELIG
                     EPDATSKRDIGPADSGEQEALWSRAEAIRTSAFWLLVITFGISSMGVTGLNLHVYSYV
                     TDVGYSPVIAATVMSIIASMQLASPLLWGVLADHIGPRYAATLRFVIQAIGLAIAILT
                     GNIICLYLGFFLYGIGLGGNMVLPDTLWAGYFGRRSLGSIRGMGLLVSQILAAMGPPF
                     FGFLFDITGGYGLSFAIFGAVLMTSALLSLMLKPPRKVARSNAVLE"
     gene            complement(2696..3313)
                     /locus_tag="VMZ02_09390"
     CDS             complement(2696..3313)
                     /locus_tag="VMZ02_09390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HUR72189.1"
                     /translation="MQEMLLAHIRISRTANVQSELYIAIGDEPNAFVGPDATGRNVMG
                     INVGMVKLVGDDANVYAALIGHEAAHLAKDHQASGRVRSNTLDLIGTVVGAGLGMAGV
                     PAGGLLSGLAVDAIGSSYSRDQEREADALGLDYMLANHYDPQGAIRLHELMLKSSSGF
                     RIPFLSSHPSSDERIENMRALIESKKPQDNLKPTAKSTENIVAKE"
     gene            3584..4303
                     /locus_tag="VMZ02_09395"
     CDS             3584..4303
                     /locus_tag="VMZ02_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HUR72190.1"
                     /translation="MKPILLTIIAILSFSATAAAQQRPERSRPPSISVNGEATIAAEP
                     DQAQIDIGVTTQARTAPDASRENAERLGRVLAAVKNLLGKGDEVKTSGYALNPQYRYP
                     QGGKPEIVGYTATNIVRIKTAKLDEVGKMIDVAMQAGANNINRLAFTLKDEEGARLDA
                     LKQATAKAKAKAEAIAASLGLKVVKIASVTEGERSFQPVFRQLPMARGEVAAAAAPTP
                     IEQGTVDVRSTVLLVAEVTER"
     gene            complement(4470..>4685)
                     /locus_tag="VMZ02_09400"
     CDS             complement(4470..>4685)
                     /locus_tag="VMZ02_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72191.1"
                     /translation="FDTLYKDSASGGRVLCLPLHPFVIGMPFRIKYLEMALDYMCSHE
                     GVWRTTAWEIAEWYYKNYYKDPGKFSG"
BASE COUNT         1151 a         1336 c         1247 g          951 t
ORIGIN      
        1 aggactttgt ccggtgagat cgagttgaac agtgttagcg gacatcaaca aattgagtag
       61 cgatggactg atggattaga gagttttttc ggcactccat actccaggac tcgaggactc
      121 cagtggatta ttgcttgaac cgtgagaaca cttgcagcgc attctggtcg aagatcttcg
      181 ccttgtcgtc tgtcgtgagc caaggaatgc tgtcgatgtt tggtttcacg tcgtcgtacc
      241 atttgccggt gcgcggatca gtcttggagc ctgcgcccgg gcgctcggtg ccgaacaagc
      301 agcgctccgg tccgcagatg cgaaacaaca acttcaatga ctcgatgttg tacaacgcgg
      361 tatcgaaata gagttgtttc aggcgctgat cgaaattctc ggtatcgtta gggccgcgca
      421 gctgggagcg ccaacggccg acctggtagg gcaccgagcc gccgccatgg gaaatgatca
      481 atttcaattt gggaaaatcc ttgaacacat tggagtcgac taaggaaaga atcgcgatgc
      541 tctcctcggt aataaaatga ccatggaatg actcgcgcgg attcttgcag cttgcgccgt
      601 ggaccaaacc gggaatatcc attttgacca tcttttcgta cagcgggtac caatattcgt
      661 tgcccatggc cggcgtcatg ccatcgcctt caccggggtc ggggttgatc atgatcccga
      721 caaatcccag gtcattgatg cagcgatcga tttcttcgaa agaattggtc accggcactc
      781 cggcattttg cggcaggccg ccgatggcgc ggaatttatt cggttccatt ttcacttgca
      841 gcgccagcgc gtcgttgact gcctggcagt accaatgaac gatcttctcc ggcttggccg
      901 aatgcatcat agtgtagggg cgcggcgaga taaacgcgaa atccgtgccg acttcgccga
      961 gaacttcaga atggcggtga ccatgccatt tcgcttcctg aatatgtttc tcgctaacga
     1021 ccactccgcg tggccggtga aagcccgcgc tgttgatcag cccagcctga aatgtctgca
     1081 attgctcacc ggcaacgata tgattgtgga tatcgatgac catgaaacga tcctcctgag
     1141 taaatggtca ctctacctaa cagacaaagg cgggaaggag caagcagggt tggagcgatc
     1201 gagtattgga gcaatgtccg attcgaaacc cgatcatcga ctactccagc actgcattac
     1261 tgcgggcgac cttccttggc ggtttcaaca tcaaactcaa tagcgcggac gtcatcagca
     1321 ccgcgccgaa aatcgcgaac gacaatccgt agccgcccgt gatgtcaaat aggaaaccaa
     1381 agaatggcgg ccccatggcc gcgagaatct gcgaaaccag caaccccatg ccgcgaatgc
     1441 tcccgagcga gcgccgcccg aaatagccgg cccacagcgt atcgggtaga accatattgc
     1501 cgccgagacc gatgccatag aggaaaaaac ccagatagag acaaatgata ttacccgtca
     1561 agatcgcgat ggctaaaccg atcgcttgaa tcacaaagcg cagagtcgcg gcgtagcgcg
     1621 gaccgatgtg atctgcaagc acgccccaga gtaacggcga agccaattgc atcgaagcga
     1681 taatactcat caccgtggct gcgatcaccg gcgaatagcc gacgtcggtg acatatgaat
     1741 aaacatgtaa attgaggccg gtgacaccca tgctggaaat gccgaaggtg atcaccagca
     1801 gccagaacgc cgaagtccga attgcttccg ctcggctcca cagcgcttct tgctcaccgc
     1861 tgtccgctgg gccgatatct cgtttcgaag tcgcgtcagg ctcgccgatc aactcggttg
     1921 cgccgtctgg caacagcccc atggactcgg gcgagcgacg gatcaccaat aacgctggca
     1981 ccaccaaaag caccaacgtc atgacaccga aaacgaacca agtctgacgc cagccgagcg
     2041 tcaagataag ccacgcggcg agcaccggca tgcagacttt ggcaaagccg acgcccatgc
     2101 tggagcaggc cagagcgcgg ctgcgctggc gcacgaacca ctgagcaatg atcacattga
     2161 cgaccatgta acccatcatc gaatcgccca gcgatactag ggtcaagcga atcgccgcga
     2221 actgcgcaaa gttttccaca tgggtaagaa ggaaataacc gagcgcgaca attgtcgtgc
     2281 cgatcaccat gagccagcgg ccgccgtaac ggtcgaccag cgttccaacc agcggcgcga
     2341 tgcacgcgga gataatccct tcgcccgagc gcagcaatga gaacacaccg cgcgaaatgc
     2401 ccaagtcagc ggtcagtggt tttaagaaaa tactcatcgt gctcgccaag gcgaacgacg
     2461 acgccacgtt ggcgagaaac ccgacggcaa cgatgtacca accgtaaaaa atcttgggct
     2521 tagctgtgcg gtgggctaac tctgcggcgg attccacaac atcggtttag caggaaacgc
     2581 cgggcgctcg caattgaagc gagagattcg ccaagaatcg cgttgcgaca catgccacct
     2641 ccgtgagata gctcgccttg agatggactt accctatgtc tgccagtacg ggcggttatt
     2701 ctttggcaac gatgttttcg gtcgatttcg cggtcggttt taagttatct tgcggttttt
     2761 tcgactcgat cagcgctcgc atattctcga tgcgttcgtc gctgcttgga tgactggaca
     2821 gaaatggaat gcgaaagccg ctactcgatt tcagcattaa ttcatgcagc cggattgctc
     2881 cctgggggtc gtaatggttg gcaagcatgt aatctagacc caatgcgtcg gcctcgcgct
     2941 cttggtcgcg gctgtaggac gaaccgatcg catctacagc aagaccgctg agcaaaccgc
     3001 ctgccggcac gccggccatt cccaacccgg cgccgacaac cgtgccgatg agatcgagcg
     3061 tgttcgaacg cacgcgcccc gatgcttgat gatccttagc cagatgcgcc gcttcgtggc
     3121 cgatcagtgc agcgtacaca ttggcgtcat cgccgaccaa ctttaccata ccgacattga
     3181 tgcccatgac atttcgtcca gtcgcatcag ggccgacaaa cgcgttgggc tcgtcaccta
     3241 tggcaatgta cagttcgctt tggacgttgg ccgtgcggct gattcgaata tgcgctaaaa
     3301 gcatttcctg catggtgcga gtcgatacta tgatgagaag ctcgccgttc gggccgtcta
     3361 attggacttg tggactcggc aaaagaacaa cgtccttgac ccgccaaatg agtacatgtt
     3421 gctgtgttga gcaactcacg caactcagaa gtgctaaaat tccaatgaac gatttcatcg
     3481 ctgtctcgtc taatctttga cgccatcgcc ggtacacgtt aagcgtaaga cgaaaggacc
     3541 ctactccttt agcgtgcaat catcgacaat gaaaggaccg ggcatgaaac caatactatt
     3601 aaccataatc gcgattctat cgttcagtgc taccgccgct gctcaacaac gtcccgaacg
     3661 gtcacggccg ccatcgattt cggtcaacgg cgaagcgacg atcgccgcag aacccgacca
     3721 agcgcagatc gatatcggcg tcactaccca ggcgcgcact gcgccggacg cttcgcgcga
     3781 aaatgccgaa cggcttggcc gcgtcctcgc cgccgtgaaa aatcttttgg gcaagggcga
     3841 cgaagtaaaa accagcggtt acgcgctcaa cccgcaatac cgctatcccc aaggcggcaa
     3901 gccggaaatc gtcggctaca ccgccaccaa catagtccgc atcaaaaccg ccaaacttga
     3961 tgaagtcggc aaaatgatcg acgtggccat gcaagccggg gcgaataaca tcaaccgtct
     4021 ggcgttcaca ctcaaagatg aagaaggcgc tcgcctcgac gcgctcaaac aagccaccgc
     4081 gaaagccaag gccaaagccg aagcgatcgc cgcatcgttg ggactgaagg tcgtaaaaat
     4141 cgcctcggta accgaaggcg aacgctcttt ccaaccggta tttcgtcaac tgccgatggc
     4201 aagaggcgaa gtggcggcag ccgccgcgcc gacgccgatc gagcagggaa cggttgacgt
     4261 caggtccacg gtattgctcg tggccgaagt gaccgagcgt taattttcga agtgtctcag
     4321 caataagtga tcgtctctct ctgatatttc acgctagtca gcaacaaact gctttccttg
     4381 cgattgatca tagacgcact ggagtgatga agttctggaa gtcgcgatct gacccgctac
     4441 tccaacactc catctctcca attttctcat tacccggaaa acttcccggg atctttatag
     4501 taatttttgt agtaccactc ggcgatctcc catgccgtcg tgcgccatac gccctcgtgg
     4561 gaacacatat aatcgagtgc catttccaaa tacttgatgc ggaacggcat gccgatgacg
     4621 aacggatgga gcggcaagca cagcacccgg ccgcccgagg cgctgtcttt gtagagcgtg
     4681 tcgaa
//
LOCUS       DATLCH010000316         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3498153, whole genome shotgun sequence.
ACCESSION   DATLCH010000316 DATLCH010000000
VERSION     DATLCH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3498153"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..939)
                     /locus_tag="VMZ02_09405"
     CDS             complement(121..939)
                     /locus_tag="VMZ02_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR72192.1"
                     /translation="MKRVAHRTTLRVIVGVASLMQSLLWSSPSAYASCRDPDVARGFG
                     RFAYAANVDATIQYFGHNFFLITTSKGTRIVADPLGPGWYPNPNVAADVVTVGKEMFN
                     HNAVQIVQGNPLILRGLKNYGADWNLVSTTVKDVLIYNVPIHQNAEFIQGIHGSAFVF
                     DTGLLCIAHLGDLSQKLNEQQIKAFGKVDIALTPIGGGRTMAPDLAKEVLAQLKPKIA
                     IPMHHRDSPYLINQFTAGMKTQLLNTDTLAVSKDALPLPTEIRVLLPRGAMNYR"
     gene            complement(1158..2093)
                     /locus_tag="VMZ02_09410"
     CDS             complement(1158..2093)
                     /locus_tag="VMZ02_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72193.1"
                     /translation="MPLPAADDKTVELAKKEGRVAFYTSMGADESKLVADTFQTKYPS
                     IRVEITRLGSEKLLQRVITESRAGSNFFDAVTNSGMEIYLLGKMGLLARYLTPDFSSF
                     MADAKDPAGRWVDMYSNLRLVAYNTKLVSKEKIPKRYEDLLDPIWKGQIGFPEGQFSW
                     FATMLKILGEERGRKFFHGLARQELNYRGAQVLVTQMIAAGEFNAGFTYDTQVLRFRN
                     RGAPIEAAPMPFVTKNIHPIALAAHAPHPNAGKVFIDHVLSKEGQMFIKNMGRVISRS
                     DIPQEEFARMKIISEDVTIADRLHAVMDDYKKYLR"
     gene            complement(2207..>2670)
                     /locus_tag="VMZ02_09415"
     CDS             complement(2207..>2670)
                     /locus_tag="VMZ02_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72194.1"
                     /translation="NGFIESATTEGTGLGGGMIAIAAFNALAEHGIPDMFPKLRVGFI
                     ETGASWVPFLLADLMAKKLRRTFLPVDFKEDLFRKNRFYVACQTNDDLPYILKYGMED
                     NLMIGTDYSHADQSAEIHALDVIEERGEKGDIPAAVARKILDDNPRRFYAL"
BASE COUNT          606 a          709 c          743 g          612 t
ORIGIN      
        1 cgatttgcta catttcctgc ggcgcgaggc agaacccgct gcgtgcaaat gcttgatgtg
       61 cgaccgagcc actgcaagta cgaaacgatc ctcaatgaag tctctccgaa cgaagccaac
      121 tcagcgataa ttcattgcgc cccgcggcag caacacgcgg atctccgtcg gcaacggcag
      181 cgcatccttg ctgactgcca atgtatcggt gttcaaaagc tgcgtcttca tgccggcggt
      241 gaattggttg atcagatagg gactgtcgcg atgatgcatc gggatcgcga tcttcggctt
      301 caactgggcg agcacttcct tggctaagtc cggtgccatg gtacggccgc cgccgatcgg
      361 cgtgagggcg atatcgactt tgccgaaagc tttgatctgt tgctcattta gcttttggct
      421 caggtccccg aggtgggcga tgcacaataa cccagtgtcg aaaacaaatg ccgaaccgtg
      481 aattccttgg atgaactcgg cgttctggtg aatcggcacg ttgtagatca aaacatcttt
      541 gacagtcgtg ctgacgagat tccagtcggc gccgtagttt ttcagcccgc gcaaaatcag
      601 cggattgccc tgcacgatct gcacagcgtt gtggttaaac atctctttgc cgacggtaac
      661 aacatcggcg gcgacattgg gattgggata ccaaccgggt cccaatggat cggcgacgat
      721 gcgcgtacct ttactggtcg taatgagaaa aaaattgtgg ccgaagtatt gaatcgtcgc
      781 gtcgacattg gccgcatagg cgaacctgcc gaagccacgc gcgacatccg gatcacgaca
      841 cgatgcataa gcggatggac tcgaccagag cagcgactgc atcaagctag ccacgccaac
      901 gatcactcgg agagttgtcc tgtgtgcgac gcgcttcacg gcattctcac acacgattct
      961 gatcagggtt ttctcgccat ggcggtgtat tcatgtgtag caggctattg gatcggcacg
     1021 taaaaaacaa attgccgccg agaacgactg aaatgattag tgattctgtt tgagagagaa
     1081 atcaagcgcg cgccaaaact aatggtaaaa gtttgtctta cccagcggcg cttcgaccgg
     1141 acgaactggc tggcgcgcta acgcaagtat ttcttgtaat cgtccatcac cgcatggagt
     1201 cggtcggcaa tggtgacgtc ttcggagatt attttcatcc gagcaaactc ctcttgggga
     1261 atatcactgc gcgaaatcac ccggcccatg ttcttgataa acatctggcc ctctttagac
     1321 agcacatggt cgataaaaac cttaccagca ttgggatgcg gcgcgtgagc ggccagcgcg
     1381 atcggatgaa tattcttggt cacgaacggc atcggcgcgg cttcgatggg cgcgccgcgg
     1441 ttacgaaagc gcagcacctg ggtatcgtac gtaaaaccgg cattgaactc gccggcggcg
     1501 atcatctgcg tgacgaggac ctgcgcgccg cgatagttta gctcctgccg cgccagcccg
     1561 tgaaaaaact tgcggcctct ctcttcgcca agtattttca acatcgtggc gaaccaggaa
     1621 aactgccctt cgggaaaacc gatctggcct ttccatatgg gatcgagcag gtcttcgtaa
     1681 cgtttaggaa ttttttcctt cgatactagt ttggtgttgt atgcgaccaa gcgcagattt
     1741 gaatacatat cgacccatcg ccccgcggga tctttggcat cggccatgaa cgaggagaaa
     1801 tccggcgtta aatagcgcgc cagcagaccc atcttgccga gcaggtagat ttccatgccg
     1861 ctgttggtga cggcatcgaa aaagttactg ccagcgcggc tctcggttat gacacgctgc
     1921 aaaagtttct cgctgcccaa gcgggtaatt tcgacgcgga tcgacggata cttggtttgg
     1981 aaagtgtcgg cgacgagctt gctttcatcg gcgcccatcg aggtgtagaa cgcgacgcgg
     2041 ccttcttttt tagcgagctc gacggttttg tcatcagccg cgggtagcgg catcgtcgaa
     2101 agagccgcgt agaaaataag cagtatggaa aataattttg tcggcgcgat cctcatccag
     2161 tcctcaaatt ggctgccgcc gctacttagg gactctgtac gagcgcctat agggcgtaaa
     2221 atctgcgcgg attatcgtcc aggattttgc gtgcgaccgc tgccggaatg tcaccctttt
     2281 ccccgcgttc ctcgatgacg tccaatgcat ggatctcggc cgattggtcg gcatggctgt
     2341 agtcggtgcc gatcattaaa ttgtcttcca tgccgtactt taaaatataa gggagatcgt
     2401 cattggtctg gcaggcgacg tagaaacgat ttttgcggaa caggtcttcc ttgaagtcga
     2461 caggcaggaa ggtgcggcga agtttcttcg ccatcaaatc ggcaagcaga aaaggcaccc
     2521 aggacgcacc ggtttcgatg aacccgactc gcaatttggg aaacatatcg ggaatgccat
     2581 gctcggccag cgcgttgaat gccgcgatgg caatcatgcc gccgcccagt cccgtgcctt
     2641 cggtggtggc ggactcgatg aacccgttgg
//
LOCUS       DATLCH010000317         9602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10936477, whole genome shotgun sequence.
ACCESSION   DATLCH010000317 DATLCH010000000
VERSION     DATLCH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10936477"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VMZ02_09420"
     CDS             complement(<1..882)
                     /locus_tag="VMZ02_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HUR72195.1"
                     /translation="MKRVVNFLLLVAFGFGVLLPRPAEGQEFKPSKLIETVVHTGPGG
                     GSDLFARAIAEMLQKEKLIPQRMQVVNKSGGGPAVAMAYLAEKKGESHTVGFYTGVWV
                     TNPLTTAEATVTLKDLTPVTRLVLEPAVIAVKADAPYKTMKDFIEAAKKNPKQLNQSG
                     GTITSRDNLMRLILQKATGAQWNFISFPSGGERLSNLLGGHVQMMVIEPQEAGEQIRA
                     GNLRVIATLTEKRLASMPNVPTIKEQGIDVPLIPQARGLVAPPAASKEVVQYWEGVFD
                     GFVKTPTWKQYVDQNQFE"
     gene            complement(915..2426)
                     /locus_tag="VMZ02_09425"
     CDS             complement(915..2426)
                     /locus_tag="VMZ02_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HUR72196.1"
                     /translation="MDALGNLLVGFSVALVPINLFWCFVGVVLGTVVGVLPGLGPAAT
                     IAMLLPLTLKMDHTTAIIALAGIFYGARYGGSTTSILLNMPGESSSVATCLDGYQMAR
                     KGRAGAALGISAIASFIAGTVGVLGLMLISPPLARFALRFAPPEYFALMVLGLAMVVF
                     LAGDSMVKALLALLVGLWLATIGTDLFTAESRFTFGQAKLLGGVDFIAATVGIFAVGE
                     VLVNLESRSGGEMLKLPRGFKNLLPTLQDLKDCRFAFVNGSLIGFFVGALPGAGATVA
                     SFLSYGLEKAVSRHPEKFGTGVIEGVAAPEGANNADAGGALVPLLTLGIPGGNTTAIL
                     LGGLILWGYKPGPLLIQEHPELFWGLVASMYVGNVMLLVLNLPLVPLFAQILRLPTYV
                     LHPFIIGISVVGVYSVNNSLFDVWMMALFGLLGYLMRKLDFPASALILGLVLGDVLER
                     ALRQSLMMSQGDLAILINRPISATLLSIAGVLLCLPAIRWVRAWRVEAVEREG"
     gene            complement(2450..2923)
                     /locus_tag="VMZ02_09430"
     CDS             complement(2450..2923)
                     /locus_tag="VMZ02_09430"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HUR72197.1"
                     /translation="MVQGIRDKTGFVAALCLAAFGSYVTSTGSKLAYVSDVGPGPGFF
                     PFWIGIGMLLFSCYQIILCVLAPGDKGPSAEQTNWHGSSRALAGWLAMAASIFLYRWI
                     GFAAGFVLLTCVFIVGLERRPILPALAIGVALALTFHLLFVVALGVSLPAGPWGF"
     gene            complement(3050..3520)
                     /locus_tag="VMZ02_09435"
     CDS             complement(3050..3520)
                     /locus_tag="VMZ02_09435"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR72198.1"
                     /translation="MALHCKAKDIPNRQVVRSGEQGVGSMIVKRAYGSECSLMHAVRA
                     PGYHTTPHAHAAEQLNHVLEGEIWFFVEDQAFHCKAGDFQRVPSNKIHWAWNRSINDA
                     IVVEAHSPALVAGPQSEGSIGLFGDGEKAAAKDPSQNNFVPFDWQSVERKIFGG"
     gene            complement(3539..3931)
                     /locus_tag="VMZ02_09440"
     CDS             complement(3539..3931)
                     /locus_tag="VMZ02_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR72199.1"
                     /translation="MARIVHIAVKVDDLDKATEFYEKTFGFVEVQTGQVRDHTSRHLS
                     DGSIDLALIKYDNEDSAESRAAGLGPCIHHFGIEVDDMNDSAAELAKYGCEIISAPGV
                     IPIKFRAPGGTVAEISPKARYKPAKNPS"
     gene            complement(3967..4905)
                     /gene="ispH"
                     /locus_tag="VMZ02_09445"
     CDS             complement(3967..4905)
                     /gene="ispH"
                     /locus_tag="VMZ02_09445"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002190.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HUR72200.1"
                     /translation="MSVKKVILAKPRGFCAGVEMAIETVERARKKHGAPLYVFHEIVH
                     NQYVVNEFEQKGVVFVNSLNDVPSQSNVIFSAHGVSPAIWQQAKEKQLRWAIDATCPL
                     VEKVHREVRKYAAQDYWIILVGNHKHDEIAGTSGEAPDRTLIVETVSEAAAVMPPSPE
                     KLVVLTQTTLSVDDTKEIIDVLKRRFPKMITPAKEDICYATQNRQDAVKQLAIRVDLV
                     LVIGSKNSENSNQLVKVARSLGKPAYLIDDYRGIDADWLNGINAVGITSGASVPDNLV
                     QEAVKYFQHQGAQAEQIGLVEENIHFALPSEVTGMQ"
     gene            5222..5425
                     /locus_tag="VMZ02_09450"
     CDS             5222..5425
                     /locus_tag="VMZ02_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HUR72201.1"
                     /translation="MTGTIKKLMRQKGFGFIKPDDGSEDVFFHRGSLAPRGQIEDLNE
                     GDTVQFQSRKGDKGPVAFDLKVR"
     gene            5437..7494
                     /locus_tag="VMZ02_09455"
     CDS             5437..7494
                     /locus_tag="VMZ02_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUR72202.1"
                     /translation="MEHKHTNRLIHENSPYLLQHAHNPVDWYAWGDEAFEKARADDKP
                     ILLSIGYSACHWCHVMERESFENEAIARLMNELFVNIKVDREERPDLDEIYMSAVQML
                     TGRGGWPMTLFLTPDRKPFYGGTYFPPEDRGGMPGFPRVLTGVNQAYRERPIDVEKSV
                     EQILTGLKRLSQSAETAQAFSKTIIADAAAKVAQSYDPDNGGLGQAPKFPNAGVYELF
                     LRHHHQSKSERYQEMVTHTLTKMARGGIYDHLGGGFHRYSVDAKWLVPHFEKMLYDNA
                     QLLRIYCHAYKATREPLFKSVVEETATYLLREMLHCEGGFYSTQDADSEGEEGKFFVW
                     TEAEVIEILGPQDGAIFCRIYDVSEFGNFEEKNILHPILTSEQASKYFDKDRGEIDRL
                     IAAAKRKLFNERAKRVKPFRDEKIITAWNGLVLSGLAETIKISPQRSYIDAAKQTMDF
                     IFAKLFRDGFLLHTYKDGQAKLLGFLDDYAFMAMGLLDLYEAVFDRSALDKAIALCDI
                     MLREFWDEQDGGFFYTGISHEKLISRAKPIFDGSIPSGNAMATQLLLRLFHFTGDDRY
                     YQKGEKVLRSYYDAMENQPFGFAHLLCALDLYLQKAKEVVIVGEPDNVAAAELLANIH
                     SVYQPNMTIQRASASDPLEKLSPLLSGKFQVDGKPTVYVCHNFTCSAPVTTWDELRPL
                     LEN"
     gene            complement(7567..8463)
                     /locus_tag="VMZ02_09460"
     CDS             complement(7567..8463)
                     /locus_tag="VMZ02_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HUR72203.1"
                     /translation="MENAPSMQKKGLGRGLSALIPAVPEPKAEPSQDNSPKQVAVDRI
                     TTSPFQPRRSFDEAKIDELASSIRNQGIIQPLIVRPKNDGFELIAGERRWRAAMRAGL
                     SHVPVVVRDASDHEAMQIALVENLQREDLNSIEEANGYRRLQEEFHWSQEEMAEKVGK
                     SRPAIANSLRLLTLPTEVQQEVASGNLPAGQARALLGLHTEPLILSACREVIAKGLST
                     RDTEKMVRTLLVGRRRRKQAPLLDPDLKSIVEELQRTLGTRVRLMPKARSAKGKVEIE
                     YYSAADLERIVETITKKWIVSS"
     gene            complement(8423..9217)
                     /locus_tag="VMZ02_09465"
     CDS             complement(8423..9217)
                     /locus_tag="VMZ02_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR72204.1"
                     /translation="MTKIIAIANQKGGVGKTTTTINLAASLAQESKRTLIVDIDPQGN
                     STTGLGIDRINVTASLYHVLDGTRFVGEVLCQTEIPELLLIPATKDLVGAEVELVSAE
                     RREYRLKDSLASVIAHYDYVLLDCPPSLGLLTLNALTAADSLLIPIQSEYYALEGLSA
                     ILETMNLVQKSLNPALHLEGIVITMFDSRNRLSHQVLEEIRSHFPDKLFNAIIRRNVR
                     LSESPSYGKPICLYDPTSTGALDYRDLAKELINRGERTVNAEEGVG"
     gene            complement(9380..>9602)
                     /locus_tag="VMZ02_09470"
     CDS             complement(9380..>9602)
                     /locus_tag="VMZ02_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HUR72205.1"
                     /translation="VRQNDIVEKFRKMENVRLPENIDYSQISGISREVCEKLIRIKPR
                     SLGQASRISGITPAAITLLSFYLKKQKTA"
BASE COUNT         2249 a         2537 c         2612 g         2204 t
ORIGIN      
        1 ttcgaattga ttctgatcga cgtactgttt ccaggtcgga gttttcacaa atccatcgaa
       61 aacaccttcc caatactgca ccacttcttt agacgctgcc ggtggtgcca ccaagccgcg
      121 agcctgcggt atcaacggca cgtcaatgcc ttgctcttta atggtcggaa cattgggcat
      181 agacgccaaa cgtttttccg tcagggtggc gattacgcgc agattaccgg cgcgaatttg
      241 ctcgccggct tcctgaggtt caatcaccat catttgcaca tgaccgccga gtagatttga
      301 caaccgttcg ccgccgctcg gaaaagaaat aaagttccac tgggcgccgg tggctttttg
      361 caagatcaag cgcattaagt tgtcgcgcga ggtaatcgta ccgcccgatt ggtttagctg
      421 tttgggattt tttttcgcgg cctcgataaa atctttcatg gtcttatagg gcgcgtccgc
      481 tttgaccgcg atcacagcgg gctccaaaac caagcgtgtg acgggtgtta aatctttgag
      541 cgtcacggtc gcttcggccg tcgtcagtgg gttggtcacc caaacacccg tgtagaaacc
      601 gaccgtgtgg ctctcgcctt ttttctcggc gaggtaagcc atcgcgacgg ccgggccgcc
      661 acctgacttg ttgacgacct gcatgcgttg gggaataagt ttctctttct gcaacatttc
      721 agcaatggcg cgggcgaaca gatccgagcc cccgccgggc ccagtgtgga ccaccgtttc
      781 aattagcttg cttggtttaa actcttgacc ctcggcaggc cgcggcaata gaacgccgaa
      841 tccgaatgcg accaatagca aaaagtttac gactcgtttc atcgataacg ctccttgtgt
      901 tgggatcgac tcttttaacc ttcccgttcg actgcctcga cacgccaggc gcgtacccag
      961 cgaatcgcgg gaaggcacaa taacaccccg gcaatcgaca gcaatgtcgc cgagattgga
     1021 cgattgatca aaatcgcaag atcgccctgc gacatcatta gcgactggcg tagcgcgcgt
     1081 tcaagtacgt cacctaaaac caggcccaag atcaacgccg aagcgggaaa atcgagcttg
     1141 cgcatcaaat atcccaataa tccgaacaac gccatcatcc agacatcgaa caaactgtta
     1201 ttgacgctgt agacgccgac gacggagatg ccgatgataa acggatggag cacataggtg
     1261 ggcagacgca aaatttgcgc gaacagcggc accaggggca aattgagcac caagagcatt
     1321 acattaccga catacatgct cgccacaaga ccccaaaaca gttcgggatg ttcctggatg
     1381 agtagtggac ctggtttata gccccataga atcaaaccgc cgagaagaat tgccgtggtg
     1441 tttcccccag gaatccccag ggtcagtagt ggcacaagcg cgccaccggc gtcggcattg
     1501 ttggcgccct cgggcgcggc gacgccttcg ataacgccgg tgccgaattt ttccggatgg
     1561 cgcgataccg ctttttcgag tccgtaagat aaaaaagacg cgaccgtggc tcctgcgccg
     1621 ggcagagcgc cgacgaaaaa gccgatcaga gaaccgttca caaaagcaaa ccggcagtct
     1681 ttgagatctt gcaatgtcgg caacagattt ttaaaacctc ggggtaactt gagcatttca
     1741 ccgccggagc gggattccaa attcaccagc acttcaccga cggcaaagat gccaacggtg
     1801 gcggcgataa agtcgacgcc tccaagtaac ttcgcctggc cgaaggtaaa gcgggattcc
     1861 gcggtgaata aatcggtccc aatggtggcg agccataagc cgacgagtag cgccaacagc
     1921 gctttgacca tggaatctcc ggcgagaaac accaccattg cgaggcccag taccatcagg
     1981 gcgaaatatt ccggcggcgc gaaacgcaaa gcgaagcgcg ccagtggggg cgagatcagc
     2041 attaggccga gcactccgac tgtgccggcg atgaacgaag cgatggcgga aatacccagg
     2101 gccgcgccgg ctctgccctt gcgcgccatc tgatagccat cgagacaggt ggcgacggac
     2161 gaagattcac cgggcatgtt aagtaatatc gacgtcgtcg agccgccgta gcgagcgcca
     2221 taaaagattc ccgccagggc gatgatcgcc gtggtgtgat ccattttgag agtcagaggc
     2281 agcagcatgg cgatggtcgc agctggtccc aagccgggca gcaccccgac caccgttccc
     2341 agaactacac cgacgaaaca ccaaaataga ttgatcggaa ccagcgcaac cgaaaagcca
     2401 accaagagat tgccaagtgc gtccatgatt gtgccgtcgg ttaggaaagt cagaagcccc
     2461 aagggccggc gggaagcgaa actcccagcg cgacaacaaa gagcaaatga aaggtgagag
     2521 ccagagctac accgatggca agcgctggaa gaatgggccg acgctctagt ccgacgataa
     2581 aaacgcacgt gagtaggaca aacccggcgg cgaagccgat ccagcggtaa agaaaaatcg
     2641 atgccgccat ggcgagccac ccggcgagag cgcgacttga accgtgccaa ttggtctgct
     2701 cggctgatgg acctttatcg cccggcgcca acacacacag tatgatctga tagcaggaga
     2761 agagcagcat gccgatgccg atccagaagg ggaaaaaacc ggggcccgga ccgacgtcgg
     2821 agacgtaggc cagcttggag ccggtggaag tgacatagct gccaaacgca gctaggcata
     2881 gcgcggcaac gaaaccggtc ttatcgcgaa ttccctgaac catcagccgt gagtgccgtt
     2941 attacgcctg atcagacttt ttcacaagcg tttgtcgata cttgggtgct cagcgaactc
     3001 gcagggccgc gccgcggtgc tactgtgttc agactttgaa agagtttaac tacccgccaa
     3061 agattttccg ttccacgctt tgccaatcaa acgggacgaa gttgttttgg ctggggtctt
     3121 tcgccgctgc tttttcacca tcgccgaaga gaccgatgga accttcactc tggggccctg
     3181 cgactagcgc cggagaatga gcctcaacaa caatggcgtc gttaatggaa cggttccacg
     3241 cccagtgaat tttgttcgac ggcacgcgtt gaaaatcgcc ggctttgcag tggaacgctt
     3301 ggtcttcgac gaagaaccag atctcgcctt cgagtacgtg attgagctgc tcggcggcat
     3361 gggcatgggg tgtcgtgtga tagccaggtg cgcgcaccgc gtgcatcagg ctgcattcgc
     3421 tgccgtaggc gcgctttacg atcatcgatc ctacgccttg ctcgccgctg cggactactt
     3481 gccgattggg aatatctttc gccttgcaat gaagcgccat tatcgattct ccttcgcctt
     3541 acgacgggtt ttttgctggc ttgtagcgcg ctttgggaga tatctcggcg accgtaccgc
     3601 caggggcgcg aaatttgata gggatcacac cgggggcgct gataatttcg cagccatatt
     3661 ttgccagctc cgccgcggaa tcattcatat catcgacctc gataccaaag tgatggatgc
     3721 acggccccag gccggcggcg cgggactctg ccgaatcctc attgtcatac ttgatcaaag
     3781 ccaggtcgat gctaccgtcg gataaatggc gcgaggtatg atcacgcacc tgaccggttt
     3841 gcacttcgac aaacccaaag gttttttcgt aaaactccgt cgccttgtcg agatcgtcga
     3901 ctttgactgc gatgtgaacg atgcgcgcca tgagacctcc cgagcttaac taaagtttcg
     3961 tatccgttac tgcatgccag tgacctcgct gggtaatgcg aaatggatgt tttcttcgac
     4021 gagcccgatt tgttcggcct gcgctccctg gtgctgaaaa tacttcactg cctcttgcac
     4081 caaattgtcg ggcacggaag cgcccgaagt gatgcccacc gcgttaattc cattcaacca
     4141 atccgcatcg atgccgcggt aatcatcgat caagtaggct ggtttgccca ggctgcgggc
     4201 gacctttacc agttgattgg agttttcgct gttcttcgaa ccaatgacca aaaccaagtc
     4261 gaccctaatg gccaattgct tgaccgcgtc ctggcgattt tgcgtagcgt aacatatgtc
     4321 ctccttcgcc ggcgtgatca ttttcggaaa gcgtcgcttg agaacgtcga tgatctcttt
     4381 ggtatcatcg acgctgagcg tcgtttgcgt aaggaccacg agtttttccg ggctcggcgg
     4441 catcacggct gcagcttcac taacggtttc tactatcagg gttcggtccg gcgcctcgcc
     4501 gctcgtgccg gcgatctcat catgcttatg attgccgacg aggattatcc aataatcttg
     4561 cgcggcatac ttgcgcactt cacggtgaac cttttcgacc aacggacagg tggcatcgat
     4621 tgcccagcgt agctgttttt ctttggcttg ctgccaaatt gccggcgaca ctccgtgcgc
     4681 gctgaagatt acgttggatt ggctggggac gtcgttcagc gagtttacaa aaacaacgcc
     4741 tttttgttcg aactcgttga ccacatactg gttatgaaca atctcgtgaa agacatagag
     4801 gggcgcgccg tgctttttgc gggcgcgctc gacggtctcg atggccatct cgacgccagc
     4861 acaaaagccg cgtggcttgg caagaattac ttttttgacg ctcatacacc tattcacgag
     4921 tctggatgaa tcgccctggg gtgtcaactc gctgaacaat gccgatcatg ccgatcgatt
     4981 acggaatcgg ataggccatt ggtgattgcg gtcagtatcc aggtagcgtg acagttggcg
     5041 agcgacttcg atcaggtcgc agagcacggt gggaatcgct cgctggcagt gcgtgccgcg
     5101 gcgcgctgct cgtgatccga attgcactga gtggtgtctt ggttatatgg gttgatcagt
     5161 cggcgaacac gggtcttgag tgtttgtaat ggagagttac cagtagagag gagaattggc
     5221 gatgaccggt acgatcaaaa agcttatgcg acaaaaaggt tttggcttca tcaagccgga
     5281 cgacggcagc gaagatgtgt tttttcaccg cggcagtttg gccccgcgcg gtcaaattga
     5341 agacttgaac gagggtgata cggtgcagtt ccaatcgcgg aaaggcgata agggcccagt
     5401 ggcgttcgat ttgaaagtac gctgaaacgt tctgccatgg aacacaaaca taccaatcgg
     5461 ctgatccatg aaaacagtcc gtatctgctg caacatgcac ataacccggt cgactggtat
     5521 gcctggggcg atgaagcctt cgagaaagct cgggcggacg acaaaccgat tctcttgagt
     5581 atcggctatt cggcttgcca ctggtgtcac gtgatggagc gtgaatcctt cgagaatgaa
     5641 gcgatcgcgc gtttgatgaa cgaattgttc gtcaatatca aagtcgatcg cgaagagcgc
     5701 ccggatctcg atgagatcta tatgagcgca gtgcaaatgc ttaccggccg gggcggctgg
     5761 ccgatgaccc tgtttcttac gccggacagg aagcctttct acggcggcac ctatttccca
     5821 ccggaagatc gtggcggtat gcccggtttt ccccgcgtgc tcacgggagt caatcaggcc
     5881 tatcgcgaac gcccgatcga tgtggagaag agcgttgagc agattctcac tggtctaaag
     5941 cgcctgtccc aatcggcgga aaccgcacag gccttttcca agacgatcat tgccgacgca
     6001 gcggcgaaag tcgcccaaag ctatgacccg gataacggcg gtctagggca ggcgcccaaa
     6061 tttcccaatg ccggtgttta tgaactgttt ttgcgccatc atcatcaatc taagagtgag
     6121 cgctaccagg aaatggtgac gcataccctg acaaaaatgg cccgtggcgg gatctacgac
     6181 catctcggtg gcggctttca ccgctactcc gtcgacgcta aatggctggt gccgcatttt
     6241 gaaaaaatgc tttatgacaa cgcgcagctt ttgcggatct attgccacgc ctacaaagct
     6301 acccgcgagc ctttgttcaa atccgtcgtc gaggaaacgg cgacgtattt gctccgtgaa
     6361 atgcttcatt gcgaaggggg attttattcg actcaggatg ccgacagcga gggcgaagaa
     6421 ggaaagttct tcgtgtggac tgaagcggag gtgatcgaaa tacttggacc acaagacggc
     6481 gcgatatttt gccggatcta cgacgtcagc gagttcggca actttgaaga gaagaatatt
     6541 ttgcatccga tcctgacgtc cgagcaggcg agtaaatatt ttgacaagga tcgtggtgag
     6601 attgatcggc tcatcgccgc cgcgaagcga aagctattta acgagagagc aaaacgcgtc
     6661 aagccgtttc gtgatgagaa aatcatcacg gcatggaacg gccttgtgct ttcgggtctg
     6721 gccgagacga tcaagatttc gccgcaacgc tcctatatcg atgccgcgaa gcagacgatg
     6781 gattttattt ttgcgaagtt gtttcgcgac ggctttcttt tgcacacgta caaagacggg
     6841 caggctaaac tgctgggctt tcttgacgac tatgcgttca tggccatggg actgcttgat
     6901 ctctacgaag cggtcttcga ccgttcagct ctcgataaag ctatcgctct ttgcgatata
     6961 atgctgcggg aattttggga tgaacaggat ggcggcttct tttacacggg gatatcccac
     7021 gagaaattga tcagccgggc taaaccgatt tttgacggat cgataccctc aggtaatgcc
     7081 atggcaacgc agctattgct gcggttgttc cacttcacag gcgacgatcg ctattatcag
     7141 aaaggcgaga aagtgttgcg ctcttactat gacgcaatgg agaaccaacc gttcggattc
     7201 gcccacttgc tatgtgcgct ggatttgtat cttcaaaaag cgaaagaagt agttatcgtc
     7261 ggcgaaccgg ataatgttgc agccgcggaa ttgctagcga acattcattc cgtctaccaa
     7321 ccgaacatga ccattcagcg cgcttctgca agtgacccgt tggaaaagct atcgccgtta
     7381 ttgagcggca agtttcaagt cgatggtaaa ccaaccgtct atgtttgcca caactttacc
     7441 tgctcagcgc cggtgacgac ctgggatgaa ttaagaccgc tactcgagaa ttaacggctg
     7501 tcgtttgagt tgggttcgga ccaaccgtca ggtttctctc gtggaaccca tgagacgagt
     7561 ggaaatctac gacgagacga tccatttctt agtaatcgtt tcgacgatgc gttcgagatc
     7621 cgcggcggaa taatattcga tctcgacttt tcctttggcc gaacgtgctt tgggcataag
     7681 gcgcacgcgg gttcctagag tacgttgcaa ttcttcgacg atcgatttta agtcaggatc
     7741 gagcagcggc gcctgtttgc gacgtctacg cccaaccaat agcgtgcgca ccatcttttc
     7801 ggtatcacga gtggacaagc ctttggcaat tacctctcgg catgccgaaa gaattaacgg
     7861 ttcggtgtgt aagcccagca acgcgcgcgc ttgcccagcc ggcagattac cggaggctac
     7921 ttcttgctga acttcggttg gtagcgtgag aaggcgcaag gagttggcga ttgccggacg
     7981 actcttgccg actttttcag ccatctcttc ctggctccag tgaaattcct cctgcaagcg
     8041 acggtagccg ttggcctcct cgatcgaatt gagatcttca cgctgaagat tttccaccag
     8101 agcgatctgc atcgcttcgt ggtcgctggc atcgcgaacc actacgggga catggctcaa
     8161 accggcgcgc atagcggcac gccagcggcg ttcgccggcg atcaattcaa aaccgtcatt
     8221 tttaggccgg acgatcagcg gttgaatgat gccttgattg cgaatggacg acgccagttc
     8281 gtcgattttg gcctcgtcga agctgcgtct tggctgaaac ggactagtgg taatccggtc
     8341 gacggcaacc tgtttcggcg aattatcttg tgaaggttcg gctttgggct cgggcacagc
     8401 gggaataagc gcgctgagcc ctctacccaa ccccttcttc tgcattgacg gtgcgttctc
     8461 cacgattaat caactccttt gccaaatctc gataatccaa cgctcctgtc gaggttggat
     8521 cgtataaaca gatcggtttg ccgtagctcg ggctttcact gagcctcacg ttacgccgga
     8581 tgatggcgtt aaacagtttg tcgggaaagt gagagcgaat ttcctccagc acctggtgcg
     8641 acaagcgatt gcggctgtcg aacatggtaa tcacaatgcc ctcgagatgc agcgccggat
     8701 tcaaagattt ttgcaccaga ttcatggttt ccaaaatcgc gctcaaacct tcgagggcgt
     8761 agtactcgct ttgaatcggt atcaaaagtg agtcggccgc cgttaaggca ttgagtgtaa
     8821 gtaatccaag cgatggcgga caatcgagca atacatagtc gtaatgcgcg atcaccgaag
     8881 ccagcgaatc tttcaggcga tactcgcgac gttcggcgct gacaagctca acttcggcgc
     8941 ctacgagatc ttttgttgcc ggaatgagaa gcagctccgg gatctctgtt tggcacagca
     9001 cctcgccgac aaatcgtgtt ccatccaaga cgtggtaaag gctagccgtt acattaatcc
     9061 gatcaattcc gagtcccgtc gtgctatttc cttgcggatc gatatcgaca attaacgtcc
     9121 gtttcgactc ctgcgccagc gaagcggcca gattgattgt tgtcgtggtt ttgccgacgc
     9181 cgcccttctg attagctatg gcgattattt tagtcacaga aaagctttat ttttcaataa
     9241 gttaggtacc tgacgcctaa gtgtatctct atcacaaagt agccttaatg gaaagaagtt
     9301 tttttgtttc acgtgaaaca aataattgtg atactagttc aaagtttcca aatcgaaaag
     9361 ttcgcagctg acggcgaggt taggcggttt tctgcttctt taaataaaat gacagcaaag
     9421 ttatggcagc aggagtgata ccggagattc gcgacgcttg gcccaacgac cgtggcttaa
     9481 tgcgaatcag cttttcacaa acctcacggg agatcccact gatctgtgag tagtcgatgt
     9541 tttcgggaag ccgaacgttt tccatttttc gaaatttctc gacgatgtcg ttctgccgaa
     9601 cg
//
LOCUS       DATLCH010000318         6883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10945100, whole genome shotgun sequence.
ACCESSION   DATLCH010000318 DATLCH010000000
VERSION     DATLCH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10945100"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VMZ02_09475"
     CDS             <1..580
                     /locus_tag="VMZ02_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72206.1"
                     /translation="GRVAVLEAEENEMILQDGKTVAKGTGKTRRIEVDCVIYAIGDQV
                     DASLGLPFSRGAFVTNPEKLPGDPNPAAYQPYDPDAKQVLDGIFVAGWSRNASVGLVG
                     VAKQDAERGMKVVNEYLAARPGLSAGEMETKIEGLIGTLKQKGVTVVTKPDVELLEGA
                     EQEEAKARKTWDFKYTSDDKMLEVIAARRAAK"
     gene            complement(702..1442)
                     /locus_tag="VMZ02_09480"
     CDS             complement(702..1442)
                     /locus_tag="VMZ02_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR72207.1"
                     /translation="MNKIAVVTGGAGNIGQAVVERLGRDNYQIIILDMNRDAGNHTVG
                     VFATRGITLDFHCANLSDENAVKSAFATIISKHGRVDLLVNVAGGSLFRHKFENFPLD
                     HWRTIIDANLTSTFLCCRAVTVPMKTQNSGAIVNISSDIAYSGGENRSAYAAAKAGIL
                     GLTRTLALELAPAGVRVNAVAPGRIETAKVRSHYTDAEWQKSNQRIPLGHPGAVEDVA
                     EAVAYLASDAAKHTTGQTLHVNGGRIMQ"
     gene            1653..3611
                     /gene="acs"
                     /locus_tag="VMZ02_09485"
     CDS             1653..3611
                     /gene="acs"
                     /locus_tag="VMZ02_09485"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HUR72208.1"
                     /translation="MADKAPNKAIEVLLNEKRNFSPSKEFRKSANLKNTAVFNQARKD
                     PQAFWAKAAKELHWFKPWKKVHEWKSPWAKWFIGGKLNASYNCLDRHVHSGRKNKAAI
                     IWEGEPGDERVLTYRDVWREVNKFANVLKKLGVKKGDRVCLYMGMVPELLIAMHACNR
                     IGAPHSVVFGGFSAEALRDRVNDAQAKILVTADGGYRRGTIVPLKKNADEAMIDTPSI
                     THCVVVQRVGAESGAQMKEGRDLWWHEAMDDAPQWCEPEKMDSEDMLFILYTSGTTGK
                     PKGIVHTTGGYLTGVHLINKWVFDMKEDDVFWCAADIGWVTGHTHIVYGPMANGVTQV
                     MYEGTPDWPDKDRLWKIIAKYGVTIFHTAPTAIRTFMRWGTEFPAKHNLKSLRLLGSV
                     GEPINPEAWMWYHKFIGGGRCPVMDTWWQTETGHPLISPLPGITKLKPGSATLPLPGI
                     EADVVDENGTSVPKGGGGYLVLTKPWPAMMRTIFGDPDRFVQTYWSRFPGKYFAGDGC
                     KRDKDGYFWLLGRVDDVMNVAGHRISTMEVESALVDHQKVAEAAVIGKAHEIKGQAIC
                     AFVSLKSGIDGSNGLMDELKAHVAKKIGAIARPDEILFAAELPKTRSGKIMRRLLRDI
                     ADGRTLGDTTTLADPTVVAKLKEQYGEE"
     gene            3659..4654
                     /locus_tag="VMZ02_09490"
     CDS             3659..4654
                     /locus_tag="VMZ02_09490"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72209.1"
                     /translation="MAKKLRLILILGLMIVPQIATAQPREKVRVALGSISVNTSVIPV
                     GAQYGLFAKYGVDLEPIYMGGGMNSLAAVTSNSVQFLSAGSTATISARLGGVDITMLT
                     VQSNKLEYSILAAPEIKSPQELKGKIVTGTRPGASADSALRLMLRKWGLEPGKDVIFI
                     SVGDSQQGRMNALQRGSVVATALTPPFSGIARQMGFRELIDMRKTDIEYAGSAIAGMI
                     GYIKSHSQLVENFLKGYVESLHFFRTQKEKTVAGIMKFMKMSDRSRAEEGYEYYLDLM
                     PVMPYASVAGVKSVLQFLAASQPKAATANPEEFYDMSILKKIEDSGFAKPFAIKK"
     gene            4706..5704
                     /locus_tag="VMZ02_09495"
     CDS             4706..5704
                     /locus_tag="VMZ02_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72210.1"
                     /translation="MQRNIIPSIVLAAACLFLAQWEAQAQGLKKIRWGQTSIGASQWI
                     PWIAKDAKFYEKNGLDVEIILLRGSGQTSQAMIGGSIFASPVTTATLMTANLSGADLT
                     TVAHTVAAVQGKMLVKPEIRKPEDLKGKKVASSGLGSLGDFMYRVTMRKYGLNPDKDV
                     IWITVGSPPERLQALASGVVDAADLSYPSDAQGERMGFRVLWDARVEVPYPSMSVVTR
                     KKTIQEDRDGVMRMIRAHVEGIHFLKTQKEASLKILAKYLRTNDRELLEGSYDIYSRD
                     FIPTPYPIVSGLKLAYEQVAQRRPEINNHKPEEFVDPSFIAELDKSGFIKKLYGQK"
     gene            5749..6756
                     /locus_tag="VMZ02_09500"
     CDS             5749..6756
                     /locus_tag="VMZ02_09500"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72211.1"
                     /translation="MKSIAQSCFIGVMSLCTVFPPLSFSQDKSLKKITWGQTSISSSQ
                     WIPWIAKDAKLYEKNGLDVDIVYLRGGSGDTSKAIIAGSIFAAPVTTATLMTANLGGA
                     DLTTIAHTVKAVQTKLIGRPELKRPEDIKGKRLAMTGLGSLGDFLHRVVMKRYGINPE
                     RDVTWLTIGAPPDRIQALEAKLVEAADLSYPFDLQAEKKGYRVLWDARTEVPYPSMSV
                     VTRKKTVQEDRDNVARMMRAHVEGIHFLKTQKESSLKILAKYLRTADKELLEGSYEIY
                     KEDFLATPYPITQGLATTYEYVALRRPEIYNHKPEEFTDPSFIAELDKSGFIKKLYAA
                     K"
BASE COUNT         1592 a         1849 c         2021 g         1421 t
ORIGIN      
        1 aggtcgcgtc gcagttctag aagccgaaga aaatgagatg attctgcaag acggcaaaac
       61 cgtcgccaag ggcacaggga agacccggcg tatagaggtc gattgcgtga tttacgcgat
      121 cggcgatcaa gtcgacgcat cactcgggct gccgttctcg cgtggtgcgt tcgtgaccaa
      181 tcccgagaaa ctgccggggg accccaaccc ggcggcttat cagccgtacg atcctgatgc
      241 caaacaggta ctcgatggca tcttcgtcgc cggttggtct cgtaacgcca gtgtcgggct
      301 cgtcggcgtc gccaaacaag atgccgagcg cggcatgaag gttgtaaacg aatacctggc
      361 ggccagacct ggcctttccg ctggcgagat ggaaactaag atcgagggat tgatcggcac
      421 cttgaagcaa aagggtgtga cggtcgtgac caaaccggac gtcgaactgc tcgaaggcgc
      481 cgagcaggaa gaagccaaag cgcgcaagac ctgggacttc aaatacacca gcgatgacaa
      541 aatgctcgaa gtcattgcgg cgcgccgggc ggcgaagtaa aaattacatc attcgcgccg
      601 attgagtata taattcagca ggtaacgttt ttagcttttt taaagtgcaa agccggagac
      661 tttctccggc ttttttgttt ttatccgata gccagcagca gttactgcat aattcgcccg
      721 ccgtttacat gcagcgtttg cccggtcgta tgtttcgccg cgtcactagc taggtaagcg
      781 accgcctctg ccacgtcctc gactgcaccc gggtgaccga gggggatgcg ttggttggat
      841 ttttgccatt cagcatccgt gtagtggctg cggactttgg cggtttcgat acgcccgggg
      901 gcgacggcgt tgacgcgaac gccagcgggg gcaagctcca gcgcgagcgt gcgagtgagt
      961 cctagtatgc cggctttggc ggcggcatat gccgaacgat tttcaccgcc gctataggcg
     1021 atgtcggagg agatgttgac gatggcgccg gagttttgag tcttcatcgg caccgtcacg
     1081 gcgcggcagc agagaaacgt cgatgtgaga tttgcgtcga tgatcgtgcg ccagtggtcc
     1141 agtggaaaat tttcaaactt gtgacggaat aaactgccgc cggcgacgtt gaccaagaga
     1201 tcgacccttc cgtgcttgct gatgatcgta gcgaatgccg atttgacggc gttctcgtcg
     1261 gacagattgg cgcaatgaaa atccagcgtg ataccgcgcg tggcaaagac gccgaccgtg
     1321 tgattgcccg cgtctcggtt catatctagg ataatgattt gataattgtc cctgccgagc
     1381 cgctcgacga cggcctgacc gatgtttccc gcgcctccgg tgacgacggc gattttgttc
     1441 atgatcgctc cttctgctcg attgagacct atcgcaccgt tgcaacaggc gtcaagagtg
     1501 attccgcagt tgcggattgc caggctcgcc atccaaaccc ctaaatcgaa tatcgaaacg
     1561 cttgacgact gtcctcttgc caacctcttg gggctcacat agaatccggc caatttgttg
     1621 cctgtaatta acttttttgg agggcacaca tcatggcaga caaggcgccc aataaagcca
     1681 tagaggttct cctcaatgaa aaaagaaatt tttcgccttc taaagaattt cgaaaatccg
     1741 ccaatctaaa aaacaccgct gtcttcaacc aggcgcggaa agatccccaa gcgttttggg
     1801 ccaaagccgc caaggaattg cactggttca agccgtggaa aaaggttcac gaatggaaaa
     1861 gcccatgggc caaatggttc atcggcggca aattgaacgc ttcgtacaat tgcctcgatc
     1921 gtcatgtcca ctccggccgc aagaacaaag cggcgattat ttgggaggga gagccgggtg
     1981 acgagcgcgt cctgacttat cgcgacgttt ggcgcgaggt caacaaattc gccaatgtgc
     2041 ttaagaaact cggcgttaaa aagggcgacc gggtttgtct ctatatgggc atggtgccgg
     2101 agctcttaat cgccatgcac gcgtgcaacc gcatcggcgc gccgcatagc gtagtcttcg
     2161 gcggtttcag cgccgaggcg ctacgcgacc gggtcaacga cgcccaggca aaaattctcg
     2221 tcaccgccga cggcggttac cggcgcggca cgatcgtccc gttaaagaag aatgccgacg
     2281 aggctatgat cgatacgcca tcgatcactc attgcgtggt ggtgcagcgg gtcggcgcag
     2341 agtccggcgc ccagatgaaa gagggacgcg atctttggtg gcacgaagcg atggacgatg
     2401 ctccccagtg gtgcgagccg gaaaaaatgg acagcgaaga catgctgttt attctataca
     2461 ccagcggcac caccggtaag ccgaaaggca tcgtgcatac caccggcggc tatcttaccg
     2521 gcgtgcatct catcaacaaa tgggtcttcg atatgaagga ggacgatgtc ttctggtgcg
     2581 ccgccgatat cggttgggtt acgggtcata ctcacatcgt ctacggcccg atggccaacg
     2641 gtgtaaccca agtgatgtac gaaggcaccc cggattggcc ggacaaagac cggttgtgga
     2701 agatcatcgc taagtacggt gtgacaatct ttcacacggc gccgacggcg attcgcacct
     2761 tcatgcgctg gggcacggaa tttccagcca agcataattt gaaatccttg cgactgctcg
     2821 gctcggtggg cgagccgatc aatccggaag cctggatgtg gtatcacaag ttcatcggcg
     2881 gtgggcgctg tccggtaatg gacacctggt ggcagaccga aaccggccat ccgctgatct
     2941 cgcctctccc gggcatcacc aagctcaaac ccgggtcagc gaccctgccg ttacccggta
     3001 tcgaagccga cgtcgtcgac gagaatggca catcggttcc caaaggcggc gggggatatt
     3061 tggtgttgac caagccatgg ccggcgatga tgcggaccat ttttggcgat cccgatcgct
     3121 tcgtgcaaac atactggagt cggtttccgg gaaaatactt cgccggtgac ggctgtaagc
     3181 gcgacaaaga cggctacttc tggctgctcg gccgggtcga cgatgtcatg aatgtcgcgg
     3241 gccatcgcat cagcactatg gaagtcgaga gcgcgctggt cgaccatcaa aaagtcgccg
     3301 aggcggcggt catcggcaag gcgcatgaga tcaaagggca ggcgatctgt gcgttcgtgt
     3361 cactgaaatc gggtatcgat ggtagcaacg ggttgatgga cgagcttaag gcgcatgtcg
     3421 ccaaaaagat cggcgccatt gcccgccccg acgagattct cttcgccgca gagttgccta
     3481 agacccgcag cggcaaaatc atgcgccgtc tgctgcgcga tattgccgac ggtcggacgc
     3541 tcggcgatac gacgacgctg gccgatccaa cggtggtggc gaagttgaaa gaacagtatg
     3601 gggaagaatg acaaagggat taggccggga ggcgtcaggc gtgagtagga aataagcaat
     3661 ggcgaagaag cttaggttaa ttctgattct gggtttaatg attgtgccgc aaatcgccac
     3721 cgcgcagccg cgcgagaagg ttcgcgtggc gttgggttcg atatccgtca acacctcggt
     3781 gattccggtc ggagctcaat acgggttgtt tgccaaatac ggcgtcgatc tcgaaccgat
     3841 ctacatgggc ggcggcatga actctctggc ggcggtgact tccaattcgg tgcagtttct
     3901 ctccgccggt tccacagcga ccatcagcgc gcgcctgggc ggggtggaca tcaccatgct
     3961 cacggtgcag tcaaacaagc tcgaatatag cattctcgcg gcgccggaaa taaagagtcc
     4021 ccaagagttg aagggtaaga tcgtcaccgg gacgcggccg ggcgcctcgg ctgatagcgc
     4081 gttaagattg atgctgcgca aatggggcct cgaaccgggc aaggatgtta ttttcatttc
     4141 cgtcggcgat agtcagcagg ggcgcatgaa cgcattgcaa cgcggctccg ttgttgccac
     4201 ggctttgacg ccgccgttta gcggcattgc ccggcagatg ggttttcgcg agctcatcga
     4261 catgcgtaaa accgatatcg aatacgccgg gtccgccatt gccggtatga ttggctacat
     4321 caaaagccat tcgcagctgg tcgaaaactt tctcaaagga tacgtcgagt cgctgcattt
     4381 ttttcgcact cagaaagaga aaaccgtcgc gggtatcatg aagttcatga agatgagcga
     4441 tcgatcgcgg gccgaggaag gctatgaata ttatctcgat ctgatgcccg tcatgcctta
     4501 tgccagtgtg gccggcgtaa aatcggtgct ccaatttctg gcggcgagcc agcctaaagc
     4561 ggcgacggca aatcccgagg agttctacga catgagcatt ttgaaaaaga tcgaagacag
     4621 cggtttcgcc aaaccgtttg cgattaaaaa gtgagtttgg cgtttgaggg cgcgagtcga
     4681 tcgtaaaact cggagcgact tcttgatgca acgcaacatt attccgtcga tcgttctggc
     4741 ggcggcgtgc ctgttcctcg cccagtggga ggcgcaggcg caagggttga aaaagatccg
     4801 ctgggggcaa acttccatcg gcgcatcgca gtggattccg tggatcgcca aggatgccaa
     4861 gttctacgag aagaatggcc ttgacgtgga gattatctta ctgcgcggtt cggggcagac
     4921 atcgcaggcg atgatcggcg gtagcatctt tgcgtcgcca gtgacgaccg cgacgctgat
     4981 gacggctaat ctgagcggtg ccgacttgac gacggtggcc catactgtag ccgccgtgca
     5041 aggcaagatg ctcgtgaagc cggagattcg taagcccgag gacctcaagg gtaagaaggt
     5101 cgccagctcc ggtctgggtt ccttaggtga ttttatgtat cgcgtgacca tgcggaaata
     5161 tgggctcaac ccggacaaag acgtgatttg gatcaccgtc ggctctccgc cggaacgact
     5221 ccaagccctt gcgtcgggag tcgtcgacgc cgcggatctc tcctatccgt cagatgcgca
     5281 gggcgagcgc atgggcttcc gcgtgctctg ggatgcgcgc gtcgaggtcc cttacccgtc
     5341 gatgtcggtg gtgacgcgca agaagacgat tcaagaagac cgcgacggcg ttatgcgcat
     5401 gatccgtgcc catgtcgaag gcattcattt cttaaagacc cagaaggaag cgagcttgaa
     5461 aattctcgcg aagtacctgc gcaccaacga tcgcgaactc ctcgaagggt cctacgacat
     5521 ttacagcagg gattttattc cgacacccta cccgatcgtc agcggtctca agctcgctta
     5581 tgagcaggtg gcccagcgcc ggccggaaat taacaatcac aaaccggaag aattcgtcga
     5641 tccaagtttc attgccgagt tggacaaaag cggctttatc aaaaagctct acggtcagaa
     5701 gtaagatagc cgtcagcatt gaggcgcgag catgaggagg aatctgtcat gaaaagtatc
     5761 gcgcagtcat gtttcatcgg cgtgatgagt ctctgcacag tgttcccgcc actgagcttc
     5821 agccaagaca aatcgctcaa gaaaatcacc tggggtcaga cgtcgatctc ttcgtctcaa
     5881 tggatccctt ggatcgccaa ggacgccaag ctttacgaaa agaacggtct cgacgtcgac
     5941 atcgtttatc tgcgcggcgg ctcgggggac acctcgaagg cgatcatcgc tgggagcatc
     6001 tttgccgcgc cggtaaccac cgccacgttg atgaccgcca atctcggcgg tgccgatctc
     6061 accaccattg cgcacacggt caaggcggta cagaccaaac tcatcggacg gcctgagctc
     6121 aaacggcccg aagacatcaa aggcaaacgg ctggcgatga ccggcttggg ctccctcggc
     6181 gatttcctcc atagagtcgt gatgaaacgg tacggcatca atcccgagcg cgacgtgacg
     6241 tggctcacca tcggcgcacc gccggaccgg atccaagcgc tggaagcaaa actggtcgaa
     6301 gcggcggatc tgtcctatcc gttcgatctg caagccgaga aaaaaggtta ccgcgtgttg
     6361 tgggacgcgc gcaccgaagt gccatatccg tcgatgtcgg tggtgacccg caagaagacg
     6421 gttcaggaag accgcgacaa cgtggcgcgg atgatgcgcg ctcatgtcga aggcattcac
     6481 tttcttaaga cgcagaagga atcgagccta aaaattctcg ccaagtatct gcgtaccgcc
     6541 gacaaggaat tactcgaagg gtcctacgaa atttacaaag aggatttcct ggcgacgccc
     6601 tatccaatca cccagggatt ggcgacgact tacgaatacg tcgcgttgcg acgcccggag
     6661 atttacaatc acaaacccga agagttcacc gaccctagct tcatcgccga gctcgacaag
     6721 agcggtttca tcaagaagct gtacgcagcg aagtagcgaa gtcgaaagcg cgaagcttgg
     6781 agttgttgtc catcatgaca ctgcgtgcac aaacatttct ggtcgcctgc ctgatatcgg
     6841 ctttggtatt ttcctacgct gcccaggcgc aagataagcc act
//
LOCUS       DATLCH010000319         4147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8713358, whole genome shotgun sequence.
ACCESSION   DATLCH010000319 DATLCH010000000
VERSION     DATLCH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8713358"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VMZ02_09505"
     CDS             <1..518
                     /locus_tag="VMZ02_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72212.1"
                     /translation="LHPELKGRMGVSVSDPAYKVVGAMLRTKGQEFVKKLKVQEIALH
                     TIIPPALLELIASGEVLASPGIFRNHTLNAIAKGAPVEWVPMDIVPTNVGGAAIAVQP
                     PHPHGALLLADYLLGPEGQAVLDKYEYGSAAKNLGFKKWRPDHGISTDKYEKELERWE
                     KLLKEISRRAM"
     gene            584..1630
                     /locus_tag="VMZ02_09510"
     CDS             584..1630
                     /locus_tag="VMZ02_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72213.1"
                     /translation="MQVLVLLVLSLLLPTQLFAQRKSMTISELVTYTGKDREAVLYAG
                     AKSEGKATWYTSLAGDSYKGMIKAFESKYAGVKIEAYRANGGEITNRMVEEGKARKSI
                     VDTIETTEANLMFQREAFLLRPYHSPYFASYPEDAKVRGERGLYFWGTARESYIGFAY
                     NKTLLPKDAVPKNYEGLLHPTLKGRMGLTIDDTSTKSIAGMLKIRGPEFLKKLKGQDI
                     SLHTIIPPALLDLIASGELLAGAAIFRNHVIVAKEKGAPVEWVPMDVVPTNVGGVAIA
                     AQPPHPHAALLLADYLLGPEGQGILGKYYYGNASTNYGFKKWRPERDYPLDKYEKELE
                     RWEKLLKETGRKGA"
     gene            complement(1863..2870)
                     /locus_tag="VMZ02_09515"
     CDS             complement(1863..2870)
                     /locus_tag="VMZ02_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72214.1"
                     /translation="MTIAVRLSVSLALFFTLLGFAAPAAAQDKPLKKIYWGVSTLSPT
                     LWIPWIAKEAKLFEKNGLDVEAVLLNGSGRTSQAMLSGSLFAASVALPQVMLADLTGA
                     DLVNVAHGIGVQGSRLMVRPEIRKVEDLKGKRIGISSLGSAGDLLFGYVLRKYGIDTT
                     REVVWLAVGNTAERLQALMSGAVDAADMTYPADIEAERKGFRALLDARKEIVYPTASV
                     VTRRKTIKDDRDTVMRFVRSFVEGIAYLKQNKEFSKKVLTKYMRTTDPEYLEGSYNIF
                     REDFISVPYPITKELDAVYEIAAYRRPEIRTHKPEEFVDTSFVAELDKSGFIKKLYEG
                     K"
     gene            complement(3068..>4147)
                     /locus_tag="VMZ02_09520"
     CDS             complement(3068..>4147)
                     /locus_tag="VMZ02_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72215.1"
                     /translation="EIAFAAEPRPAWRVDWDKTVTAAEKEGSVSIYIFEAGPLTEEAV
                     QGFERAYPKIKVNQLRGRGNDLGPRLVAERRAGKYLADLFAGGKGTAYTTLYVGKMLD
                     PIKPLLLLPEVVDESKWWRGELKYVDPENKHIFVYIGNAGGVEINHNTNLVNPREFGS
                     YWDLIQPKWKGKIVAVDPRMRGMDNPVLFFYYHSKLGPDFIKKLYGDMEVSIARDYRQ
                     PTDWLASGKFAICIPCVSDEMDKAMEQGLPVGQIFRLKEGGTLSSSGGTLSLLQNAPH
                     PNAAKVFVNWLLSRDGQMHVQKGRKGRPRTGSNSLRIDISKNEIPEEIQRREGVDYFD
                     ADDERHADRRPADKLFNEIFGKTAK"
BASE COUNT          967 a         1207 c         1077 g          896 t
ORIGIN      
        1 tgctccatcc cgaactcaaa ggacgcatgg gtgtgtcggt aagcgatccc gcttacaaag
       61 tagtcggcgc catgctgcga accaagggcc aagagttcgt caagaagcta aaggtgcaag
      121 aaatcgctct gcacacgatc atcccgccgg cgctgttgga actgatcgcg tccggcgaag
      181 tgctggcatc gcccggaata tttcgcaatc atactctcaa cgccatcgcc aaaggtgcgc
      241 cggttgagtg ggtgcccatg gatatcgtgc cgaccaacgt cggcggcgcg gccatcgcgg
      301 tgcaaccgcc ccatccgcac ggcgccttgc tgctcgccga ctacctgctc ggcccggagg
      361 ggcaggcggt tttggacaaa tatgaatacg gcagcgcggc aaaaaatctc ggcttcaaaa
      421 aatggcggcc agaccatggc attagcaccg acaaatacga aaaagagctg gagcgctggg
      481 aaaagctctt gaaagagatc agccgccgcg ccatgtagaa cccgagcagg ctaatcgcgc
      541 tcgctctccg aattaatcga aaaagttgga ggaatcgtcg acgatgcaag ttttagtact
      601 cttggtccta tctctactgt tgccgactca gttgttcgcg cagcgcaagt ccatgaccat
      661 ttccgaactc gttacctaca ctggcaaaga ccgcgaagcg gtgctttacg ccggagccaa
      721 gagcgaaggc aaagcgacct ggtacacgtc acttgccggc gactcctaca agggcatgat
      781 caaagccttc gagtccaagt atgccggcgt caagatcgaa gcctaccgcg ccaacggcgg
      841 cgaaatcacc aaccgcatgg tcgaggaagg taaggcgaga aaatccatcg tcgacaccat
      901 cgaaaccacc gaagccaact tgatgtttca gcgcgaggcc tttctgctgc gcccctatca
      961 ctcgccatat tttgccagtt atccggaaga cgccaaagtc agaggcgaac gaggactcta
     1021 cttctggggg acggcgcgag agtcttacat cggcttcgct tacaacaaaa ccctgctgcc
     1081 caaagacgcc gtgccgaaaa actacgaggg tctgctccat cccaccctca aaggcagaat
     1141 gggcctgacc atcgacgaca ccagcacaaa atcgattgcc ggtatgctga aaatccgcgg
     1201 tcccgagttt ctgaaaaaat taaagggaca ggatatcagt ctgcacacga tcattccgcc
     1261 ggctttgctt gacctcatcg cctcggggga actactcgcc ggtgcggcga tctttcgcaa
     1321 tcatgttatc gtcgccaaag aaaaaggcgc tccggtagaa tgggtgccaa tggatgtcgt
     1381 gccgacgaat gtcggcggcg tcgccatcgc cgcccagccg ccgcacccgc acgccgcttt
     1441 gctgctcgcc gactacctgc tcggccctga aggccaaggc attttgggaa agtattacta
     1501 tggcaacgct tcgaccaatt acggtttcaa aaaatggcgt cccgaacgcg actatccact
     1561 ggataaatac gaaaaagaac tggaacgctg ggaaaagctg ctgaaagaaa ccggccgcaa
     1621 aggcgcgtga tctttgcgcg agactaaatg ccggacgact gaagcactat ggcgcaaaga
     1681 acacgaagat tgggaagcct caccgcagag acgccgagag cgcagagtcc ggagattctg
     1741 agtaaataaa ttccgcactc tgtggactcg acgcctctgc ggtcaatact gagctttgtg
     1801 ctctttgtgg ttaactcttt tccgactccg atttttgcat tctttacggc tcatccgatt
     1861 tgctattttc cctcatagag cttcttgatg aagccgcttt tgtccagctc ggcgacaaag
     1921 cttgtgtcga cgaactcttc gggcttgtgc gtgcggatct ccggccggcg atatgcggcg
     1981 atttcgtaga ctgcgtccag ctccttggta attgggtagg gcacggaaat gaaatcctca
     2041 cgaaatatat tgtacgagcc ctcgagatat tccggatcgg tcgtgcgcat atacttggtt
     2101 aggaccttct tgctgaattc tttgttctgt ttgagatatg ctatgccttc gacaaacgag
     2161 cgcacgaagc gcatcacggt gtcgcggtcg tccttgatgg tcttgcgccg cgtgaccact
     2221 gacgcggttg gataaacgat ctccttcctg gcatcaagca gggcgcggaa gcctttgcgc
     2281 tctgcttcaa tgtcggcggg atacgtcata tccgccgcgt cgacggcgcc gctcatcagc
     2341 gcttgcaaac gttccgcggt attgccgacg gccaaccaga cgacctcccg tgtggtgtcg
     2401 atgccgtact tgcgcaacac gtacccaaac agcaaatccc ccgcggagcc cagactcgaa
     2461 atgccgatgc gcttaccctt aagatcttcg accttgcgaa tttccggccg taccatgagg
     2521 cggctaccct gcacaccgat accgtgggcg acgttcacca gatcggcgcc ggtcaaatcc
     2581 gccagcatca cttggggcaa tgccacggat gccgcaaaca aactgcccga caacatcgcc
     2641 tgtgaagttc gcccggaacc gttgagcaat accgcttcga catcgagtcc gttcttttcg
     2701 aaaagtttcg cctctttggc gatccaagga atccacaaag tcggcgataa agttgaaacg
     2761 ccccaataga tttttttcag cggcttgtcc tgtgccgccg ctggcgccgc aaaacccagt
     2821 agcgtaaaaa ataatgcgag tgaaacgctc aacctaaccg cgatggtcat ggtgtctcca
     2881 agttgacatg cagtggaatc ggagatctcc gccgcgaggg tgctaagcgc aaatggaagt
     2941 taaaagttga cagatcatct tttaacaact ccggcagcct tgcgagcttg gcgtctttgc
     3001 agaaaaactc caggttccaa ttttctagtt cgtgatctcc gtgctctttt gtttagatct
     3061 tttccggtta ctttgccgtt ttgccgaaga tctcattgaa caacttatcc gcggggcggc
     3121 ggtcggcgtg tcgctcgtca tcggcatcga agtagtcgac accttcgcgg cgctgaattt
     3181 cctctggaat ttcattctta gaaatgtcga tacgcagcga attcgacccg gtacgcggcc
     3241 gacctttgcg ccctttttgc acatgcatct ggccatcgcg cgataacaac caattgacga
     3301 acactttggc cgcgttagga tgcggcgcgt tctgcaacaa gctcaaagtg ccaccggaag
     3361 aactgagggt cccaccctcc ttcagccgaa aaatctgccc caccgggagg ccctgctcca
     3421 tcgccttgtc catttcatcg gacacacagg gaatgcaaat ggcaaatttc cccgaggcga
     3481 gccagtcggt cggctggcga tagtcgcgcg caatcgacac ttccatatcg ccgtacagtt
     3541 tcttgatgaa gtcgggaccc aacttactgt ggtagtaaaa aaacaacact gggttgtcca
     3601 tgccgcgcat gcgcggatcg acagcgacaa ttttgccttt ccatttgggc tggatcagat
     3661 cccaataaga accgaactcg cgcggattga cgagattggt attatgattg atctcaacgc
     3721 cgccggcatt gccaatgtag acaaagatat gtttgttctc cggatcgacg tacttgagct
     3781 cgccgcgcca ccatttcgat tcatcgacaa cttcgggcag caaaaggagc ggcttgatcg
     3841 ggtcgagcat cttgcccacg taaagtgtcg tgtacgccgt tcccttacca ccggcgaaca
     3901 ggtcggcaag atacttaccg gcgcgccgct cggcgaccaa acgcggacca agatcgttgc
     3961 cgcgcccgcg caactggttc actttgattt ttggataagc ccgttcgaaa ccctgcactg
     4021 cctcctcggt cagcggtccc gcttcgaata tataaatcga aaccgagcct tccttctccg
     4081 ccgccgtaac tgtcttgtcc caatcgaccc gccaagccgg cctcggttca gccgcaaagg
     4141 caatctc
//
LOCUS       DATLCH010000320         4259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10946963, whole genome shotgun sequence.
ACCESSION   DATLCH010000320 DATLCH010000000
VERSION     DATLCH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10946963"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1161)
                     /locus_tag="VMZ02_09525"
     CDS             complement(1..1161)
                     /locus_tag="VMZ02_09525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR72216.1"
                     /translation="MDFNLTMEQQQIREEIKKVCREFPDAYWRKVDREKAYPDAFVKK
                     LGDLGWLAALIPEEFGGTGLGITEASVILEEINHSGGVATACHAQMYTMGTLLRHGNA
                     EQKKRYLPKIATGELRLQAFGVTEPNAGSESTRIQTTAVKKGDRYLINGQKIFISRVL
                     QSDLMLLLARTTPYDELTDKTRGLSVFIVDLKANQGKLKVKPLDLMINHHTNELFFDN
                     VEVPVENLIGAEGMGFRYIIDGWNAERILVAAEAIGDGRWFVERAAKYASERIVFGKP
                     IGSNQGVQFPIAKAYANIEAADLVRFQAATKFDRKEKCGAEANLAKYLASEAAWEAAN
                     ACLTTHGGYGFAAEYDVERKFRETRLLTVAPVSNNLVLAYLGQHVLGMPKSY"
     gene            1226..1735
                     /locus_tag="VMZ02_09530"
     CDS             1226..1735
                     /locus_tag="VMZ02_09530"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HUR72217.1"
                     /translation="MAVKPGWEGRFFEDFEIGDIYRCRLGRTVTEADNIWFTLLTNNT
                     NQIHFNNEYGKRTEFGKCLINSALTLAIVAGMGVADVSENGFALGWDGITLPNPLFAG
                     DTLYSESEVLEKRESKSKPQWGILKVRTRGVQQEGKVVIDYARSVMVWKKAFAPKNDL
                     FPEIKQDEK"
     gene            1798..2997
                     /locus_tag="VMZ02_09535"
     CDS             1798..2997
                     /locus_tag="VMZ02_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HUR72218.1"
                     /translation="MTGNSKPLRALDGVKILAFEQVLSGPFATCLLADMGAEVIKAER
                     PAVGDVIRSWDSVVKGLSSGYVWLNRNKRSLTVDVKTSQGREILQKLAQQSDIFFENY
                     APGVAGRLGLGYDNLSELNPRLIYCSLSGYGQDGPYRDVKAYDLLIQGEGGIIATTGY
                     PDKPARAGIAIADIASGMYAAIGILLALYQREKTGEGQLVDVSMLDSIVSWLGYFPHH
                     YWHAGEEPGRVGMRHHYVCPYGPYLAGDGEYVNLAVASPADWQVFCKIVIEMPEMLSD
                     ARFATVQDRRKNRNQLEETIENIFASRDHKYWLAQLKKADLPHGIVRGIAQVLAHPQV
                     IARKLIREADSPVGTIPVIANALKMSGSEARYDRIPALGENNAAILSELGYDAGAIET
                     LQRDKVI"
     gene            3059..3640
                     /locus_tag="VMZ02_09540"
     CDS             3059..3640
                     /locus_tag="VMZ02_09540"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72219.1"
                     /translation="MRKRVIGIMGASANDGLAAADKKRLQHMARELGEAIADADCVLV
                     TGATTGLPAMVAAAFRQRGGFALGISPAENNREHVQRYGLPDDGADVIVYTGFGYKGR
                     NVVNVRSSDIVIIIGGAIGTLNEFTIAYDEGKVIGVLQDSGGIADHIKKIVEVGQKST
                     QGILIFDADPKTLIQTCLSAIATRDYRADRDVK"
     gene            complement(3613..4176)
                     /locus_tag="VMZ02_09545"
     CDS             complement(3613..4176)
                     /locus_tag="VMZ02_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72220.1"
                     /translation="MNWFFIGALTALMAVSAPVENSQHRSERPAIGQLQLKTKATAPV
                     VLCIDDKPTVGGQPSGDAYAKAAAQGFRSVLTLRSPKDGVDTLRERLLVESSRLRYFN
                     IPSVNSLPNHKQTDEFLHIIREPLNHPTLINCAFAERVAPYMMIFRISEEGWSEEKAV
                     DEAVRLGMAREEMRKFARGYLTSRSAR"
BASE COUNT          925 a         1106 c         1227 g         1001 t
ORIGIN      
        1 tcagtaagac tttggcatgc ccaagacatg ttggccgaga tacgcgagca cgagattatt
       61 gctgaccggc gccacggtca acaaacgcgt ctcgcgaaac ttacgttcga cgtcgtattc
      121 tgccgcaaag ccgtagccgc catgagtggt taagcaggcg ttggcggcct cccaggctgc
      181 ctctgaggcg agatactttg ccaaattagc ctcagcaccg catttttctt tgcgatcgaa
      241 ttttgtcgcg gcttggaagc ggactagatc cgcggcttcg atgttcgcgt aggctttagc
      301 gatcgggaat tggacgcctt ggttggatcc gatcggttta ccgaagacaa ttcgttcgct
      361 ggcgtatttg gcggcgcgct cgacgaacca acggccgtcg ccgatcgctt cggctgcaac
      421 gagaatccgc tcggcattcc agccgtcaat gatgtagcgg aagcccatgc cctccgcgcc
      481 gataaggttc tcaaccggca cttcaacgtt gtcgaaaaac agttcgttgg tatgatgatt
      541 gatcatcaga tcgagcggct tgaccttgag ttttccctga ttagctttca aatcgacgat
      601 gaagaccgag aggccgcgag ttttgtccgt caattcgtcg tacggtgtgg ttctcgccag
      661 caacaacatc agatctgatt ggagcacccg cgagataaag attttttggc cattgattaa
      721 gtaacggtcg ccttttttaa cggcagtggt ttggatcctt gtcgattccg agccggcatt
      781 gggttcggtc acgccaaaag cttgcaagcg cagctcaccg gtggcgattt tgggcaggta
      841 gcgttttttt tgttcagcat tgccatgacg caaaagcgtt cccatggtgt acatctgagc
      901 atgacaggcg gtggcaacgc cgcctgaatg attgatctct tccaaaataa cgctcgcttc
      961 ggtgatcccc agccccgtgc cgccaaattc ttcgggtatg agcgcggcga gccagccgag
     1021 atcgccgagc tttttcacga atgcatccgg gtaggctttc tccctatcga ctttgcgcca
     1081 gtaagcatcg ggaaattctc gacagacctt cttgatctct tcacggatct gttgctgctc
     1141 cattgtcagg ttgaaatcca tcgatgcctc ctatagtcgg tagaatatag cctaatgttt
     1201 tttgcgccta aagaggagga tcgtcatggc tgtgaagcct ggatgggaag ggcgtttttt
     1261 cgaagatttc gagatcggtg atatttatcg ttgccgtctc ggtcgcacag ttacggaagc
     1321 cgataacatc tggtttactt tgctgaccaa caacaccaat caaattcatt tcaacaacga
     1381 gtacggcaaa cgcacggaat ttggcaagtg cttgatcaac agcgccttga ccttggcgat
     1441 tgtcgccggc atgggtgtcg ccgatgtgag cgagaacggc tttgctctgg gttgggacgg
     1501 tattacgctg ccgaatccat tgtttgccgg cgatacgctg tattcggaat ccgaagtgct
     1561 ggagaagcgc gaatccaagt cgaaacctca atggggcatt cttaaagtgc gcacccgtgg
     1621 cgttcagcag gagggtaagg tggtgatcga ttatgcgcgc agcgtaatgg tgtggaaaaa
     1681 ggcctttgcg ccgaagaacg atttgtttcc ggagattaaa caagacgaga aatagactcg
     1741 ttcaatgggt tcaaatcgtt caagccgttc aaaacgttca agcaaaccga ggtgagtgtg
     1801 accggtaatt cgaaaccgtt gcgggcgctg gatggagtta agattcttgc cttcgagcag
     1861 gtgctgtcgg gaccttttgc gacttgtttg ttagccgaca tgggggcgga ggtgatcaag
     1921 gccgagcggc cggcagtagg cgacgtgatt cgctcttggg attcggtcgt taagggattg
     1981 tcgtcaggct acgtctggct caatcgcaat aagcgcagtc tgaccgtaga tgtaaagacg
     2041 tcccaagggc gggagatcct gcagaaactc gcgcagcagt cggatatttt ttttgaaaac
     2101 tacgcgcccg gcgtcgccgg ccgactcggc ctcggctacg acaacttgtc tgaactcaat
     2161 ccgcggctga tctattgttc gctctccggt tatggccaag atggccctta ccgcgacgtc
     2221 aaagcctacg atctgctgat tcagggggaa ggcggcatca tcgcgaccac cggttatccc
     2281 gacaaaccgg cgcgtgccgg tatcgccatc gccgatattg cctccggaat gtacgcggcg
     2341 atcggcattt tgctggcgct ctatcagcgg gaaaagaccg gcgaggggca gctcgtcgac
     2401 gtttccatgc tcgattccat cgtgtcgtgg ctcggttatt ttccgcatca ctactggcat
     2461 gcgggcgagg agcccggccg tgtcggtatg cgtcatcatt atgtttgtcc ctatggtcct
     2521 tatctggccg gcgacggtga gtatgtaaat ctcgccgtgg cgagcccggc ggattggcag
     2581 gtgttttgta aaatagttat cgaaatgccg gagatgctca gcgatgcacg cttcgcgacg
     2641 gtgcaagacc ggcgtaagaa ccgcaatcag ttggaagaaa ccatcgagaa tatctttgca
     2701 tcgcgcgacc acaaatactg gttggcgcaa ctgaaaaaag ccgatttgcc gcacggcatc
     2761 gtgcgcggca ttgcccaagt gctagctcat ccccaggtta tcgctcgcaa gctgattcgg
     2821 gaagccgact cgccggtggg aaccatcccg gtgatcgcca atgcgcttaa aatgtcgggt
     2881 agcgaagcgc gctacgatcg cattccggca ttgggtgaga acaatgccgc cattttatcc
     2941 gagcttgggt acgatgccgg ggctatcgaa acgctgcagc gcgataaggt tatttagttc
     3001 aatctggcat gcctgagacg aacgcagtgt gccacgaatc tgtgctgggg atctgaatat
     3061 gcgtaagcgc gtcatcggga tcatgggtgc atccgcgaat gacggcttag ctgcggcgga
     3121 taaaaaacgg ctgcagcaca tggcaagaga gttgggcgaa gccatcgccg acgccgattg
     3181 cgtgcttgta accggcgcca ccaccggtct gccagccatg gtcgcggccg cgtttcgaca
     3241 gcgcggcggc ttcgcgctgg ggatatcacc tgccgaaaac aaccgcgagc atgtgcagcg
     3301 ctacggcttg ccggacgatg gcgccgatgt gatcgtttac acgggttttg gctacaaggg
     3361 gcgcaacgtc gtcaacgtcc ggtcgtcgga tatcgtgata atcatcggtg gcgcgattgg
     3421 aactctcaac gaattcacca ttgcctacga tgaaggaaaa gtgatcggtg tcttacaaga
     3481 cagcggtggg atcgcggatc acatcaagaa aattgtcgag gtcggccaaa aatcgactca
     3541 gggtatactg atattcgatg ccgatcctaa aactctgatc cagacgtgtc tgagtgcgat
     3601 cgcgacgcgc gattatcgcg ctgatcgtga cgtcaaatag ccccgggcaa acttgcgcat
     3661 ttcttcccgc gccatgccca atcgaacggc ttcgtccacg gctttctctt cgctccagcc
     3721 ttcttcgctg atgcggaaaa tcatcatata aggggcgacc cgctctgcga acgcgcagtt
     3781 gatcaacgtc ggatgattca acggctctct gatgatgtga agaaattcgt cggtttgctt
     3841 gtgattcggc agtgagttga cactcggaat attgaaatag cgcaggcggc tggattccac
     3901 caacaatcgc tcgcgcaggg tatcgacgcc gtctttcggc gatcgcaaag tgagaactga
     3961 gcgaaagccc tgggccgctg ccttggcgta agcgtcgcct gacggttgac cgccgacggt
     4021 tggcttgtcg tcaatacaca acacgaccgg cgcagtggcc ttagttttta actgaagctg
     4081 gcctatcgcg ggtcgttcac tgcgatgttg cgagttttcc accggcgcgc tcaccgccat
     4141 cagtgcagtg agcgcgccta taaaaaacca gttcatagat tgaagacgcc cgctgcggta
     4201 ctgccgagaa tggcgctttt atccgcgttg gagcagggag attggcgcac catctcgat
//
LOCUS       DATLCH010000321         4310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7221996, whole genome shotgun sequence.
ACCESSION   DATLCH010000321 DATLCH010000000
VERSION     DATLCH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7221996"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1458)
                     /locus_tag="VMZ02_09550"
     CDS             complement(382..1458)
                     /locus_tag="VMZ02_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72221.1"
                     /translation="MGIFATLYNYFRPATADPARLVEYLYLDQNRLSAYFEQISSTVT
                     YDKVPVWRAGLAMTGPTAQAEQARFGRSFSTHEKIHKLLEHIKRLPPVNYGDGPRPDP
                     AFRLETIPAMKVVIPAKPGAASNQTLVLWLSAMPGFDIPRGRRLVLIQEYRQTDKEYV
                     EHVSPKTALVELLSAAKEQLIQTGTYEPLVATLQGPFLFHLPATFEKEFAGREHGRCT
                     RAVRAEFATHGVELTESATWSSLNDLRSLPAPWEVEIADDGRRYLLRSHNEKHDGVDL
                     TGEISVCENVRHEIAARFATKPGEVLQQLDAAVSESRLITTLYRVRSWMIEGIMPNDL
                     EYTFGYPIFIAEGRAFNSQGGRSV"
     gene            complement(2002..3951)
                     /locus_tag="VMZ02_09555"
     CDS             complement(2002..3951)
                     /locus_tag="VMZ02_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HUR72222.1"
                     /translation="MAPRIDTSLFDRITQGRAQQLSVPGGGFQPPSESERDRERRFAN
                     DEVVLAFIAIPQVALMAAGAFLWWYLGGAMVPLAVGLLGGAIIAGCLYWMARSRLQDR
                     QQVSRFDLFWCLKWAGAVLLGIFLSVLIGGLLQRIWEHFGIVFMVLTYVVFTPIVRRS
                     YDAGESGKVEWFFVGSGLVFAPFFVAWSIASISWVPFTTQNSLRGWLDRQSASVRGVL
                     TARLNPERCGNWTIDGQPIRVAVTLSGGGYRAALIHAGFLASLDAQCVPVHVLSTVSG
                     GSIIGASYAMGVPPAQFAWQLATKQPGLPADIIKIVPVMSDLLLSFLGNITETYTDHF
                     RRTFFGDMKLADLPDNPLLLVNVTDLQSGGDNAREVFFKKRAPSARFNGKSLDDSLKV
                     AQIVAASGAFPGAFRPATLPWVEPQTRHSSSLEVKERQFVDGGVIDNLGVEGLRHYLT
                     LPRADGNLPERPDLLIISDASALVGPELMSPSADAMTLIKRTTDYTYDLLHRHIYARY
                     TGQGDLAHWIRSTAIADQVAAVNYRQIDSRLGGDDPAELQSVAIPSTAPVMAELLARY
                     PTCRIDDGVTGEAVQKRVAGFATLYELSPRQVEEAFWLGRVMGQLYGPAIQCARKKTL
                     GEPCSKVGSDVGLVLQCPRRRQLFQ"
BASE COUNT          996 a         1259 c         1142 g          913 t
ORIGIN      
        1 gcagtttcaa caacctcaat cgcttcgcga taatccatca cgccttttaa ctcttcgggt
       61 cgtatgagca ccaccattaa atttcgcctc gctttcgcgc tttatctcag ggcttgattc
      121 ctcctctagt acaggagagc agcggaaaaa ggaacccacc agacagtcca tgccgtttca
      181 ctagccctca tgcgctacag gtgggcgcac ccgctggaca gactgtgccc attgccgatt
      241 catctgattc ctcgcaagcg ttacagccca ctaaggttcg ccgaaatgaa aggcgtcgat
      301 gtcggtgcgt aactgcgcaa gcaattggct agatcagaaa gcctgagcca tagctttact
      361 gacgagctcg gccattcacg ttcaaacgct tcttccgccc tgtgaattga aggcccgccc
      421 ttcggcgatg aatatcggat aaccgaaggt gtactcgaga tcgttcggca tgattccttc
      481 tatcatccag gagcggacgc ggtagagcgt cgtgatcagg cgcgattcgc tgacggcggc
      541 gtcgagctgt tgcaggactt cacccggctt ggtcgcaaac ctcgcggcaa tttcgtggcg
      601 cacgttctcg cagacggaaa tctcgccggt aagatcgacg ccgtcgtgtt tttcgttgtg
      661 agagcgcaaa agatagcgcc ggccgtcgtc ggcgatctcc acctcccaag gcgcgggcaa
      721 tgatctgaga tcgttcaggc tgctccaggt tgccgactcg gtgagctcga caccatgggt
      781 ggcgaactcc gctcgcaccg ctctcgtaca gcggccatgt tcgcggccgg cgaactcttt
      841 ctcgaaggtc gccggcaggt gaaacaaaaa aggtccttga agggtggcta ccagcggttc
      901 gtaggtccct gtctggatca gttgctcttt ggctgcggag agcaattcga ccagcgcggt
      961 tttaggcgac acgtgctcga cgtactcctt atcggtctgt cgatattcct gtatcaggac
     1021 caagcgccgg ccccgaggga tatcgaaacc gggcatcgcg gaaagccaga gaaccaatgt
     1081 ttggttcgat gcggcacccg gtttcgccgg tatgacaact ttcattgccg gaatggtctc
     1141 cagacggaac gcgggatcgg gccgcggccc gtcgccgtaa ttcaccggcg gcaaacgttt
     1201 gatgtgctcg agcagcttgt gaattttttc gtgggttgaa aagctgcggc caaatctcgc
     1261 ctgctccgct tgcgcggtcg gaccggtcat agccaatccg gcacgccaga ctggcacctt
     1321 gtcataagtg accgtgctgg agatttgttc gaaataggcg ctcaaccggt tctggtcgag
     1381 atacagatat tcgaccagcc gtgccggatc ggcggtcgca ggccggaaat aattataaag
     1441 cgtcgcgaaa attcccattc ctcactcgct tcattttgcc gctgctccaa tcgcacacag
     1501 ctcttttgca tcttagccgc cagattgcga gacaaactgc gtcaccggca cagagtcaag
     1561 tgccacactt aacccatacg caaccgttct cacatgaaac agaagtcaaa ttcgttttga
     1621 tcggcacttt catctcctca atgaagcgat caatttcgca cacgaggctc tcgtcaacga
     1681 gttctagaaa agtactgttc gaactcaacg ccctcagtat gtaacccttc aggaagccaa
     1741 gaccgacacc gacacacttc acgtccgcta agtgtgcggc aatctttccc ttcataacca
     1801 aaagtttcca ccgcgtgtcg aaacgaattg gtaaaatgct ttcatagacc cctgcgagcg
     1861 agatacggtc gtctataacc cgcgcatgaa gatcacgtat cttcggtggc actgtaacaa
     1921 atttgactat gaggctattc aaatcatctt cagtgatgac aaagctaagg cgtactattt
     1981 ccattcgtca gttatctctg ttcattgaaa taattgtcgc cggcgcgggc attggagaac
     2041 cagccccacg tccgacccga ctttgctaca cggctcgcca agcgttttct tacgcgcgca
     2101 ttggattgcc gggccataga gctggcccat gacccgaccc aaccaaaacg cctcttccac
     2161 ctggcgcggc gacagctcgt agagtgtggc gaagccggcg acccgtttct gcaccgcctc
     2221 gccggttacg ccatcgtcga ttcgacaagt tgggtagcgt gccaggagct cggccatgac
     2281 cggagcagtg ctgggaatcg ccaccgattg caattccgcc ggatcgtcgc cgccgagtcg
     2341 ggagtcgatc tgtcgataat taaccgcagc gacttggtcg gcgatcgccg tcgagcgaat
     2401 ccagtgagcc agatcgcctt gtcccgtata acgagcgtaa atgtgccgat gcagtagatc
     2461 gtaagtataa tccgtcgtgc gcttgataag cgtcatagcg tcagcactgg gagacatcag
     2521 ctccggtcct acgagcgccg aagcgtcgga gataatcagg agatcggggc gctcaggcaa
     2581 atttccgtcg gcccgaggca atgtcaggta atggcgcaaa ccttcgacgc cgagattgtc
     2641 gataacaccg ccatcgacga actgtcgttc ctttacctct agactactac tgtggcgggt
     2701 ttgcggttcg acccacggta atgtcgctgg cctgaaagca ccgggaaacg cgcctgacgc
     2761 cgcgactatc tgcgccacct tcaaactatc gtcgagactt ttgccattga acctagccga
     2821 cggcgcgcgc tttttgaaaa acacttcacg ggcgttatcg ccgcccgact gcaaatccgt
     2881 cacgttgacc agcaagagcg ggttgtccgg caggtcggct agtttcatgt cgccgaaaaa
     2941 cgtccggcga aaatgatcgg tgtaggtttc ggttatgttt cctaaaaagg acagcaagag
     3001 atcgctcatg accggcacga tcttgatgat gtcggccggc aggcccggtt gcttggtcgc
     3061 gagctgccag gcgaactgag ccggcggcac gcccatggca tacgatgcgc cgatgattga
     3121 gccgcccgac actgtcgata ggacgtgcac aggcacgcat tgagcatcta gcgatgcaag
     3181 aaacccggca tgaatcaatg cggcgcgata acctcctcct gaaagcgtga ccgcgacacg
     3241 aatcggctgc ccgtctatag tccaattgcc gcagcgctcc ggattgagcc gcgcggtgag
     3301 aacgccgcgc acgctcgcac tctgccgatc aagccaacct cgcagcgagt tttgcgtcgt
     3361 gaacggcacc catgagatcg atgctatgga ccaggcgaca aagaaaggtg cgaagaccag
     3421 acctgagccg acaaagaacc attcgacttt tccactttct ccggcatcat acgaacgacg
     3481 cacgattggc gtgaaaacga cgtaagtaag aaccatgaag acgatgccaa aatgctccca
     3541 tattcgttgg agcaacccgc cgatcaaaac gctgaggaaa atgcccagaa gcaccgcgcc
     3601 ggcccacttc aagcaccaga acagatcgaa acgacttacc tgctggcggt cttgcaggcg
     3661 ggagcgcgcc atccagtaga gacagcctgc gatgatcgcg ccgccgagca aacctacagc
     3721 gagcggaacc atcgcgccgc ccagatacca ccacaagaat gcacccgctg ccatcaaagc
     3781 aacttgagga attgcaataa acgcgagaac cacctcgtca ttggcgaatc gccgttcgcg
     3841 atctctttcg ctttctgacg gcggttgaaa accgccaccc ggcacggaca actgctgcgc
     3901 ccgcccttga gtaatccgat caaacagaga ggtatcgatt ctgggagcca tctaaattat
     3961 gaccagcggc gtcatatcgg cgcgcggcat tattgacggt gcgagtatcg gtttatcgac
     4021 gcaaaccgtt tggccactta tttacgcgat cttgtagcgc cgaacaatag tttgcgcagg
     4081 cgctactgcg caaatttcct ctggcagtac ccaacctccg gtctcaacaa tttacgttgg
     4141 ccggaccgag aaagcggact gaaatgccgc catgaacaaa ttgatgccgt tgttaattct
     4201 cctgcaattg cttccaaccg cagctcaagc gtttcaattc gacatgtgga cttccgggat
     4261 atccagccgc gaagcgatgg cgattgcgag ggacaaagat attcctgtcg
//
LOCUS       DATLCH010000322         7754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7222117, whole genome shotgun sequence.
ACCESSION   DATLCH010000322 DATLCH010000000
VERSION     DATLCH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7222117"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="VMZ02_09560"
     CDS             <1..1781
                     /locus_tag="VMZ02_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HUR72223.1"
                     /translation="IEDYAMIGDCHTAALVARNGSIDWLCLPRFDSGACFAALLGNEE
                     HGRWLLAPEGEVRGVSRRYRDGTLVLETDYETLDGAVTVIDCMPLRSKEPDLVRIVVG
                     KRGKVRMRMQLIIRFDYGSIIPWVRRSKDGLSAVAGPDTLILHTGVDLHSEGFRTEAE
                     FTVAEGQRIPFVLMWHPSHEPAPPTMDAEVLVEHTEQWWREWSNRCNYQGEWREVVLR
                     SLITLKALTYAPTGGIVAAPTTSIPEQLGGVRNWDYRYCWLRDATFTLYALMLGGYLE
                     EACAWREWLLRAVAGNPSQINIMYGLSGERRLTELELRWLPGYAGSAPVRIGNAACEQ
                     FQLDVYGELMDAMHLARRSGISTDENDWRVQCALVNFLESAWREPDNGLWEMRGPKRH
                     FTHSKIMAWVAFDRMVKAVERFGCDGPVERWRQLRSIVHEEVCRRGFDSTRNTFVQYY
                     GGKTLDASLLMIPLVGFLPAGDTRVRGTVQSIECDLMADGFVQRYRTNQEIDGLPPGE
                     AAFLSCSFWLADNYAMLGRYQEARGLFERLLSLCNDVGLLSEGYDPKKHRLLGNFPQA
                     LSHLALVNTAFNLSRAHGPAADRQSS"
     gene            2086..3315
                     /locus_tag="VMZ02_09565"
     CDS             2086..3315
                     /locus_tag="VMZ02_09565"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72224.1"
                     /translation="MFLNTTFRAFRHRDYLIYWLGLFLGHTGTLVQTTAQTWLIFQLT
                     NSPFYLGLEGLCLGLPRVLFSAFGGAVVDRGDRKIIFVLTQSSFVAMALFLGLMQHLG
                     LIRVWHLLMVSALTGFLVSFEQPVRQSILHQLVPRDVLPNAVTLYQMVFNGSMLFGPS
                     VGGLLIPIIDTKGCFFLATAGNLLVLLTIFLIHIPAAGPSTGKTSITRDMIDGLKLAW
                     NTPVFLALFTVLAVVSFCTKPYTQFMPVFAQTILNVGAPGLGMLLMAPGAGAILGGLT
                     LASLRSFPRPAHLLFGLAAGFGISIALFATSRSFSLSLIFLFIAGGFQTTFLSSVATL
                     LQFNTSEHNRGRIMSMFGLINRGLGPMGSFPFGLIATWIGAPWTVAICGALTISLVGY
                     VVNHRACLTDARPVGDL"
     gene            complement(3530..4081)
                     /locus_tag="VMZ02_09570"
     CDS             complement(3530..4081)
                     /locus_tag="VMZ02_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72225.1"
                     /translation="MTLATEPLEKLVKLIGDEARVDARMRETQTALALVKRRVSESLA
                     QHYIATREVRFQMPEDLMREEQSYERLLQALQDMKGEIAKQIRPVEEQIIQANVDHLR
                     QSFGQESRRLSKCIEEVDDNILACRQYLQEHERIRASLAAINQKLIQLGAEGVQVPDG
                     LNTSDLGEIVRQRIEHLQSHGKI"
     gene            complement(4307..4642)
                     /locus_tag="VMZ02_09575"
     CDS             complement(4307..4642)
                     /locus_tag="VMZ02_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR72226.1"
                     /translation="MNKEDFYNQGMEHFAQDHLDDAVAAYLRALDEDPDYTDALHALA
                     MTYSHQEKLDKAIETAKRLIKAAPDDELAYTSLSIFYQQSGMIAEAEEVAAKARTLGW
                     KRQLSDNKG"
     gene            complement(4672..5793)
                     /locus_tag="VMZ02_09580"
     CDS             complement(4672..5793)
                     /locus_tag="VMZ02_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR72227.1"
                     /translation="MALPLQYFQKLVESCPDIIISVDRHGTITFYNDGARQNLGFSSD
                     EVLGKNVLEIYPTVEEARKVMAAMRANDGEGRGRVRNFETRFKTKRGEPVPVTISASI
                     IYDDRGQEIGSIGFAKDIREIRRRDRLVTLAEIAIGLSHEINNSLEVLVNQTELMKKF
                     VDRVANDEDYIVEADRLDSVASQVRKIQDITTRMAKMAEEGEYGTTEYLDGKMMTDLH
                     VDAAASDKPCARPAPDARYPLSGLSVLVVDDDANICQSLKQLLESERCCVETASSGVY
                     AMEWLGRRRFDVVLSDVVMPDMDGYELYQTVKKNMPELPVVLMTAFNYDKDHIIKRSC
                     LQGLQGVIFKKPVNPMMLKKVLLQQCRPEQIQRPSTSTL"
     gene            5904..7742
                     /locus_tag="VMZ02_09585"
     CDS             5904..7742
                     /locus_tag="VMZ02_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HUR72228.1"
                     /translation="MPVTHDTRQLSWTLAWAVVFCDIGTSVYYVPGILYGNVGNKTPF
                     FILLTTLGFIPLALKYIEITWRNPEGGGVVTMTTKAFGPMWGCLGGMLIITSYFLTAA
                     ISTVSGFHYIASVVHSLDDHIVVLACVGLLALAVLNVIGIRESATAALLMAVSAFVVD
                     LGVIVLSVKDFSSDQWRQALTSLSPGPETTTKNLLVGFSAAWLAFSGLESISQLSPTM
                     RLPLRKTTRWAMFAVIGTMLVTAPLLSLLSINMLSPEFKSTQSERFISQIASISGGFG
                     LQMAVVISASSLLLFAANTAIIGAYHIFLALAKSDFLPRVIALRSTKFNSPHIAILIA
                     TIVPMAVVVATQGELALLGEMYAFGLIGAFVFSSFSLDVIRWRLGRRDLGFWIGILTT
                     AMLLLAWCVNLVEKELATLFGGTLTVIGMVIAVGVRRSWFVDILHRIPAIQRLQQRAY
                     LASEALVDEELKGLVTLADAVELKALYPATTLLALRGETPALIQQGIHLARGKGETAL
                     YCVYVEEWPGLFHGDAPHRPNEEGIRTLRAALHEVRDKNIEVIPIWTVSHNAADAIAN
                     AAKTLDVDAVLIGQSRRSAFYHMLRGHVVKGLTKKLPRDCHLIIFN"
BASE COUNT         1578 a         2160 c         2101 g         1915 t
ORIGIN      
        1 ctatcgaaga ctatgcaatg atcggggact gtcacaccgc cgcgctggtg gcgcgcaacg
       61 gctcaatcga ttggttgtgc ctaccgcgct tcgattccgg ggcgtgtttt gccgcgctct
      121 tgggcaacga agaacacggt cggtggctcc tggcccccga aggagaagtt cgcggtgtta
      181 gtcgtcgcta ccgcgacggc acgctcgtgc ttgaaaccga ttacgagact ctagacggcg
      241 cagtgacggt tattgattgc atgccgctac gcagcaaaga accagacttg gttcgtatcg
      301 tggtcgggaa acgcggcaag gtgcgcatgc gcatgcagct gataattcgt tttgactatg
      361 gatccattat tccttgggtg cggcgcagca aagatgggct gagcgcggta gccggcccgg
      421 acacgcttat tctgcacacc ggggtcgatc ttcacagtga gggctttcgc accgaagcag
      481 agttcaccgt tgcggaagga caaaggatcc cttttgtgct catgtggcat ccgtctcatg
      541 agccagcgcc gccgacgatg gatgccgagg tgttggttga acacacggag caatggtggc
      601 gggagtggtc taaccgctgc aattatcagg gtgagtggcg cgaagtcgtg ctgcgttctt
      661 tgatcacact caaagcgctt acctatgcac cgaccggcgg cattgtcgcc gctcccacca
      721 cctcgattcc ggagcagctc ggcggagtgc gcaattggga ctatcggtac tgctggctgc
      781 gcgatgcgac ttttactctt tatgctttaa tgctcggagg atatctggag gaagcgtgcg
      841 catggcgcga atggcttctg cgcgccgtcg ccggtaatcc gtcgcaaatc aatatcatgt
      901 acggactatc cggggaacgt cgcctcacgg agttggagct ccgctggttg ccaggctatg
      961 cgggttcagc tcccgtgcgc atcggtaatg ccgcgtgcga acagttccaa cttgatgtct
     1021 acggtgagtt gatggacgcc atgcatctgg cgcgccgcag cggaatttca accgatgaga
     1081 atgactggcg ggtgcaatgc gcgctggtca attttctgga gtccgcctgg cgagaacccg
     1141 ataacggttt atgggagatg cgtggtccaa agcggcactt cacgcattcc aaaataatgg
     1201 cttgggtcgc ctttgatcgc atggttaagg cggtggagcg ctttggttgc gatggaccgg
     1261 tcgagcgttg gcgacagctc cgctcgatcg tccacgagga agtatgccgc cgcggctttg
     1321 actcgacgcg taatactttc gtccagtact acggcggaaa gacactcgat gccagcctgc
     1381 tgatgattcc gctagtggga tttctccccg ccggtgacac acgcgtacga ggcaccgtgc
     1441 agtcgatcga gtgcgatctg atggccgatg gcttcgttca acgctatcgg acgaatcagg
     1501 aaatcgatgg gctgccgccc ggcgaggcgg cttttctctc ctgtagtttc tggctggcgg
     1561 acaattacgc catgctcggg cgctaccagg aggctcgcgg gttattcgag cgcctgttga
     1621 gcctgtgtaa cgacgttggt ctattatcgg aaggatatga tccgaaaaaa caccggctgc
     1681 tgggcaattt tccgcaagcg ctgagccacc tggcgttggt caacacggcg tttaatctgt
     1741 ctcgtgcaca cgggcctgct gccgaccgcc agagcagtta atcataagcc gcgcatgtcg
     1801 caagcgacgc attttgccat ccgcctcgca gagtccgtca atggcagccg gtttattgaa
     1861 aagagtctga gcttgtgaat acttaagaag cgactacagg gaattgcgaa ttagtataat
     1921 cgtgaataat ttttaacgag ttacgctgaa ccttcgggtc aggagtacgg gtcagagtct
     1981 tgtaggcatc gtaaaatcat tccccttaca gcgggccatt gagccgcggc agactcgcgc
     2041 ggccatgatc gctcgtcgcg aatgcgcttt aaatagacga gcttcatgtt tcttaacacg
     2101 acttttcgcg cgtttcggca ccgcgactac ttaatttatt ggctcggtct gtttctcggc
     2161 cataccggga cactcgtcca gacgaccgcg caaacctggc tgattttcca actcaccaat
     2221 tcgccattct acttgggcct ggagggactc tgcctgggat tgccgcgggt tttgttttcc
     2281 gccttcggcg gcgccgttgt cgatcgaggg gatcgcaaaa taatatttgt cttaacccaa
     2341 agctcttttg tcgcgatggc gctgtttctt gggctgatgc aacacctcgg cttgattcgg
     2401 gtgtggcatc tcttgatggt ctctgctctc accggatttc tcgtgtcgtt cgagcagcca
     2461 gtgcgccagt cgatcctgca tcaactcgtg ccgcgcgatg tcctgcccaa cgccgtcacg
     2521 ctttatcaaa tggttttcaa cgggtcgatg ctctttgggc catcggtggg tggattgctg
     2581 atcccgataa tcgataccaa aggctgtttc tttttggcca cagcggggaa tctactcgta
     2641 ttgctgacca tatttctcat tcacataccc gctgcgggcc catcgacggg caaaaccagc
     2701 attacgcgag acatgatcga cggcctaaaa ctggcctgga acacgccggt gtttctagcg
     2761 ctctttacgg tcctcgccgt tgtcagcttt tgcaccaaac cgtataccca gttcatgccg
     2821 gtgtttgcgc aaactattct caatgtcggg gcgccgggtc tcggcatgtt gctgatggct
     2881 ccaggggccg gggcgatctt aggcgggctg acactcgctt cgctccgcag ctttcccagg
     2941 cctgcgcatt tactgttcgg tctcgctgcc ggctttggca tttccatcgc cctattcgcc
     3001 acgtcgcgta gcttttccct atctttgatc ttcttgttca ttgccggcgg cttccagacc
     3061 accttcctat cgtcggtcgc cactctgctg caatttaata cgagcgagca caatcgtggt
     3121 cgcatcatgt cgatgttcgg tttgataaac cgcggtctcg gtcccatggg cagctttccg
     3181 tttggactga tcgcgacttg gatcggtgcg ccttggacgg tggcgatttg cggcgccctc
     3241 accattagcc tggtcggcta cgtggtcaat cacagggcat gtttaaccga tgctagaccc
     3301 gtcggcgatc tttaagaatg ttgcggacat ccccaacgtt cagcggaggt tgctcaagaa
     3361 gcggaatctt gcgggtatta gagcgtcacc ttatacgata agcgacgatc gcacccaaac
     3421 cgaaattctt actcagttaa gatgaagcaa ttggatttgg tcactcgcga gctgccgcgc
     3481 cgctggttaa atctgccaac gccgagagag aactgtaccg ttatcgcatc tagatcttcc
     3541 cgtgtgactg caggtgttca atgcgctggc ggacgatttc cccaagatcg ctggtgttta
     3601 gaccatccgg cacttgaact ccttcggcac ctaattgaat gagcttctga ttgattgcgg
     3661 cgagactcgc gcggatgcgc tcatgctcct ggagatattg ccggcaagcc agaatgttgt
     3721 cgtcgacctc ctcgatgcac ttgctcaacc ggcggctttc ctggccaaaa gattgtcgca
     3781 gatgatcgac attggcttgg atgatctgct cctcgacggg gcggatctgc ttggcaatct
     3841 cgcccttcat atcttgcagg gcctggagca agcgttcgta tgattgttct tccctcatga
     3901 gatcctccgg catctgaaat ctaacttctc tcgttgctat ataatgctgc gccagcgact
     3961 cggacacccg ccgtttaacc agtgccaggg ccgtctgagt ttcacgcata cgcgcatcca
     4021 cacgggcctc gtcgccgata agtttaacca atttctctaa cggttcggtc gcgagcgtca
     4081 tgactctatt caggttgaga gcaggtagcg ttgacgttgc ccggctcacg gccatttatt
     4141 atccagtcgc gtgaaaaact gcaagcactt gagaatttaa gggaagatta cgaaaaccgc
     4201 gatgttttga ggagcaaaaa cgaacgataa acccgcgcac tggcccaccg gattggcccc
     4261 gctacggcct atgacaacac acaataaact ttgcgtggaa gggaaactat cccttgttgt
     4321 cgctgagctg gcgcttccag ccaagggtgc gagctttagc ggcgacttct tccgcttcgg
     4381 cgatcatgcc gctctgttga tagaaaatag acaagctggt gtaggcgagt tcatcgtcgg
     4441 gtgcggcttt gatcaaacgc tttgccgtct cgatggcttt atcgagcttc tcctggtgtg
     4501 agtaggtcat cgccaaagca tgcaaagcgt cggtgtaatc gggatcttca tcaagggcgc
     4561 gcaaataagc agcaaccgca tcgtccaagt gatcctgagc gaaatgctcc atcccctgat
     4621 tataaaaatc ttctttgttc atctgccgtg attggttaag ccaccgtgag gttacaatgt
     4681 cgaggttgac ggccgctgga tctgttcggg acggcattgt tgcagcaaga cctttttaag
     4741 catcatcgga ttgaccggct ttttaaaaat gacgccctgt aaaccttgga ggcagctgcg
     4801 tttaatgatg tggtctttgt cgtaattaaa cgcggtcatc aagaccaccg gtaactctgg
     4861 catgtttttc ttgaccgtct ggtacagctc gtaaccatcc atgtcaggca tcacaacatc
     4921 gctcaaaacg acatcaaagc ggcgccggcc caaccactcc atggcataaa ctccggagga
     4981 ggcggtctcg acacagcagc gctccgattc caagagctgc ttcagggatt ggcagatatt
     5041 ggcgtcatcg tcgacgacta aaacgctcaa gccactcaat gggtagcgcg cgtctggcgc
     5101 aggccgggcg caaggcttgt cgctcgcggc agcgtcgaca tggagatcgg tcatcatctt
     5161 accgtcgaga tactcagtcg tgccgtattc gccctcttcg gccattttag ccatgcgcgt
     5221 ggtgatatct tgaattttgc gaacttgcga agctaccgag tcgagccgat ccgcctcaac
     5281 aatatagtcc tcatcattgg cgacgcgatc aacgaatttc ttcatcagtt cggtctgatt
     5341 gaccaagact tccaaactat tgttaatttc gtgggacagc ccaatagcga tctcggcgag
     5401 cgtcacgagt cgatcgcgcc gccgaatctc gcgaatatct ttagcaaagc caatcgagcc
     5461 aatctcctgc ccccgatcgt cataaatgat cgacgccgaa atcgttaccg ggaccggctc
     5521 tccgcgcttc gtcttgaatc gggtctcaaa atttctcacc cgcccacggc cttcgccgtc
     5581 gttagcgcgc atcgccgcca tcactttgcg cgcttcttct actgtgggat agatttcgag
     5641 gacgtttttg ccgaggactt cgtctgagga aaagccgaga ttctgccgcg cgccgtcatt
     5701 atagaaagta atcgtgccgt gacgatctac tgagatgata atgtcagggc aactctcgac
     5761 tagcttctgg aagtattgta agggtaacgc cataaacggt aggcagtatc caccataata
     5821 attctaacat cgccgtaatt gcaagagacc gctccccgta aaaccacgcc gcgccatcct
     5881 tcttaaattc tctctgatcg cgcatgccag tgacccacga tacccggcaa ctttcctgga
     5941 ccctcgcttg ggctgttgtc ttctgtgata tcggtacttc ggtctactac gtacctggta
     6001 ttttgtacgg caacgtcggc aacaagacgc cattttttat cctactcaca accctaggtt
     6061 ttattccgct cgcgctaaaa tacatcgaga tcacctggcg caatcctgaa ggcggcggcg
     6121 ttgtgacgat gacgaccaag gcgtttggtc cgatgtgggg ctgcctcggt ggtatgttga
     6181 tcatcaccag ctactttctc actgcggcga tttcaaccgt ttcgggattt cattacatcg
     6241 ccagcgtggt gcattccctc gacgaccaca tcgtcgtgtt ggcatgcgtc ggcctcttgg
     6301 cgttggcggt tttgaacgtc atcggtatcc gcgaaagcgc aaccgcagcg ctgttgatgg
     6361 ccgtcagcgc ctttgttgtt gatttgggcg tgattgtcct gtcggtcaag gacttctcat
     6421 ccgaccagtg gcgccaagcg ttgacaagcc tttcgcccgg ccctgagacg acgacaaaaa
     6481 accttctggt gggcttttcc gccgcttggc tcgccttttc aggattggaa agcatcagtc
     6541 aattaagccc aaccatgcgc ctgccgctgc gcaagaccac acgctgggca atgttcgcgg
     6601 tcatcgggac tatgcttgtg actgcgccct tgctgagtct gttgtcgatc aacatgcttt
     6661 cgcctgaatt caagtccacg cagagcgaac gttttatctc gcaaatcgcg tccatctccg
     6721 gcggattcgg cctacaaatg gcggtggtga tcagcgcgtc gagcctgcta ctgtttgccg
     6781 ccaacacagc cattatcggc gcgtaccata tctttctcgc actggcgaaa agtgactttc
     6841 tgccgcgcgt cattgccctg cgcagtacca aattcaacag tccgcatatc gccatcttga
     6901 tcgccacgat tgtacccatg gccgtcgtcg tcgccaccca aggagagctc gccctgttgg
     6961 gggaaatgta cgctttcggt ttgatcggcg ctttcgtgtt ctcatctttt agcctcgacg
     7021 tgattcgttg gcgcttgggc cgccgggatc taggattttg gattggcata ttaaccactg
     7081 ccatgttgct gttagcttgg tgtgtcaatc tggtcgagaa agagttagca accctgttcg
     7141 gcggcacgct gacagtcatc ggcatggtca tcgcagtcgg cgttcggcgc tcgtggtttg
     7201 tcgacattct gcatcgaatt ccagcgattc agcgattgca gcaacgcgcc tatttggcct
     7261 ccgaggcttt agtggacgag gaactcaagg gactagtgac cctcgccgat gcagttgagc
     7321 tcaaggcact ctaccccgcc acaacactgt tggcattgcg cggagagacc ccggcgctga
     7381 tccaacaagg cattcatctc gccagaggca agggtgaaac tgcgctctac tgtgtttacg
     7441 tcgaggaatg gcccggattg ttccacggcg atgcgccaca ccgccccaat gaggaaggca
     7501 ttcgcacctt gcgcgccgcg ctgcacgagg tgcgcgacaa aaacatcgaa gtgattccca
     7561 tctggaccgt ttctcacaac gctgcggatg ccatcgccaa tgctgcaaaa actctcgacg
     7621 tcgacgccgt attgatcggc caatcgcgcc gatcggcgtt ttatcacatg ctgcgcggcc
     7681 acgtcgtcaa gggattgacg aaaaaactgc cgcgcgactg tcatctgatt attttcaact
     7741 gaagcaagtc cgca
//
LOCUS       DATLCH010000323         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10949039, whole genome shotgun sequence.
ACCESSION   DATLCH010000323 DATLCH010000000
VERSION     DATLCH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10949039"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VMZ02_09590"
     CDS             complement(<1..985)
                     /locus_tag="VMZ02_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HUR72229.1"
                     /translation="MNAFAFLSLVVLATLFPNQARAAAAADSFYRGKTIRVIVGFAAG
                     GGFDQYARTIARHMPKHIPGNPAMIVDNMTGAGSRLAANYLYKASAPDGLTIGNFIGS
                     LLLQQILGDQGVEFDGKRFEWLGAPVQDETVCALTKASGVTNLDQWLAAKKPVKLGGE
                     APGANDSDVPRMLQATLKLPLQLIEGYKGTSFIRIAAEAGEVDGGCWTWASIRSTWKK
                     GIDSGDVIPIIQVNAKKAHDIQQVPNAIDQAKTAEAKALLEAGVHAPSAILRAYALPP
                     GTPKDRLNVLRDAFNATMKDKEFVAEIVKGNLELDPLTGAEVETIARKLYQI"
     gene            complement(1209..>2301)
                     /locus_tag="VMZ02_09595"
     CDS             complement(1209..>2301)
                     /locus_tag="VMZ02_09595"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HUR72230.1"
                     /translation="ETGQRTVQTLSGMPAVVDHNNLYSETRSDRMSPVVAKHLSRVYV
                     PNRTSNDVWVIDPESLKVIDKFKVGINPQHVVPSWDLKTLWVNNNAEGRKDGSVTPID
                     PLTGRPGANIPVDDPYNMYFTPNGEAAIVVAEAHKRLDFRDPQTMATKYSIATPDCRG
                     INHADFSIDGRFAIFTCEFAQSLVKIDLIDKKVISYLRLSKGGMPQDVRVAPDGKVFY
                     VADMKAAGVFIVDADKFSEIGLIPTGIGAHGLYPSRDGTKLYVANRGVAKVYGPPKGK
                     GSVSVIDFATRKVETTWVIPGGGSPDMGNVSIDGKKLWLSGRYDNEVYIFDTTNGSVK
                     KIPVKTEPHGLTVWPQPGRYSLGHTGNMR"
BASE COUNT          462 a          607 c          677 g          555 t
ORIGIN      
        1 cgatctggta gagcttgcgc gcaatggttt ctacttcagc gccggtgagc ggatccagtt
       61 ccaagttgcc tttgacgatc tcggcgacaa attctttgtc tttcatggtc gcgttgaaag
      121 cgtcgcgcag aacattcaaa cgatctttcg gcgttcccgg aggcagcgca taggcgcgca
      181 ggatcgccga cggtgcgtga acgccggctt ccagcagcgc tttggcctcg gcggttttgg
      241 cctgatcgat ggcattgggg acctgctgga tatcgtgggc tttcttggcg ttgacctgga
      301 ttatcggaat tacgtctccc gaatcgatgc ctttcttcca agttgagcgg atcgacgccc
      361 aggtccaaca gccgccatcg acttcgccgg cttctgcggc gatgcggatg aacgaagtgc
      421 ctttgtaacc ctcgatcaat tgcagcggca gtttcaatgt tgcctgcagc atgcgcggca
      481 cgtcggagtc gttggcgccg ggcgcctcgc cgccgagctt taccggcttt ttggcggcga
      541 gccattgatc gaggttggtg actccgctcg ccttcgtcaa ggcgcacacc gtttcgtcct
      601 gcaccggtgc gccgagccac tcgaagcgtt tgccatcgaa ttctacgcct tggtctccca
      661 gtatctgttg cagcagcagt gagccgataa aattgccgat ggtcaaaccg tccggcgcgc
      721 tggctttata taggtaattg gcggcgagcc ggctaccggc gccggtcata ttgtcgacga
      781 tcatcgccgg gttgccgggg atatgcttcg gcatgtggcg cgcgatggtg cgcgcgtatt
      841 gatcgaagcc gccgccagcg gcaaagccaa caatgactct gatggttttg ccgcgataga
      901 agctgtcggc cgcggcggct gcgcgggctt gattgggaaa tagcgtcgca agcacgacca
      961 atgacaagaa tgcgaatgcg ttcatattag gctcctttgt aacgccatca gagattagac
     1021 acacagatcc gaataaaaca gtcttatcgg agttgatgcg gttcctgcaa gaagagtcta
     1081 tctactcgtc agggcggagc tcaggaggcg aatgtaactg tcaattgatt tgggaatgtc
     1141 atgcggcgaa aaatttacag cgccatgcgg ttgcaataac tggaagacgc ggagcgagaa
     1201 gcactttgtt agcgcatatt gccggtgtgc ccgagcgagt agcgcccggg ctgcggccac
     1261 acggtgaggc cgtggggttc ggttttcaca ggaatttttt tgacgctgcc gttggttgtg
     1321 tcgaagatat agacttcatt gtcatagcga cccgacagcc agagcttttt gccgtcgatg
     1381 ctaacattac ccatgtccgg gctgccgccg ccaggaatga cccaggtggt ttcaactttg
     1441 cgggtcgcga agtcaataac gctgacgctg cctttgcctt tgggcgggcc ataaactttc
     1501 gcgacgccgc gattggcaac atagagcttg gtgccatcgc gactggggta aagtccatgc
     1561 gcgccaatgc cggtgggaat aagaccgatt tcggagaatt tgtcggcgtc gactataaac
     1621 accccggcag ctttcatgtc ggcgacgtaa aaaactttgc cgtccggcgc gacgcgcaca
     1681 tcctgcggca taccgccctt ggataatctc aagtagctga taactttttt gtcaattaga
     1741 tcgattttca ccaagctttg ggcgaactcg caagtgaaga tcgcaaagcg tccatcgatg
     1801 gaaaagtcgg cgtgattgat gcctcggcaa tccggcgtcg cgatcgaata ctttgttgcc
     1861 atggtttgcg gatcgcgaaa gtcgagccgt ttatgcgctt cggcgacgac gatcgcggct
     1921 tccccgttgg gggtgaaata catgttgtaa ggatcgtcaa ccggaatatt agcacccggc
     1981 ctgcccgtca gcggatcgat gggtgtgacg ctgccatctt tgcgcccttc ggcgttgttg
     2041 ttgacccaga gagtttttag atcccaagac ggcacgacat gctggggatt aatacctact
     2101 ttgaatttgt cgattacctt gagtgattcg ggatcgatca cccatacatc gttggaagtg
     2161 cggttaggca cgtaaactcg gctcaaatgc ttggcaacga ccggactcat cctgtcggat
     2221 cgggtttcgc tatacaggtt gttgtggtca acgaccgcgg gcatccccga tagtgtttga
     2281 accgtgcgct gtcccgtctc t
//
LOCUS       DATLCH010000324         6045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7224691, whole genome shotgun sequence.
ACCESSION   DATLCH010000324 DATLCH010000000
VERSION     DATLCH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7224691"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VMZ02_09600"
     CDS             complement(<1..862)
                     /locus_tag="VMZ02_09600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HUR72231.1"
                     /translation="MVVPKVSIVIPAFNSGPLLRDTLAGIQQQTFQDFEIIVVDDGST
                     DNTQKLVTSADSSIRFYRQTNQGPAVARNRGVSLAQGEFIAFCDHDDIWNDQHLETLL
                     AVFFKNPSTAMVFDKATYCDLEDTNVAPRHTPAHEKELVSGPVSIRQIWQCCVESMSV
                     VMVRKAIFDKLGGLHPKIWGLDDLHFYLRLASQYIVRFADYNGCVKQITGQNLLPTVG
                     LEGHVKCLEDLRAHYPEVVRAIGPLRYRLRLARKYRKLARRCSTQNRQFTASQYYWQA
                     YRLNVLNVSIL"
     gene            complement(918..2663)
                     /locus_tag="VMZ02_09605"
     CDS             complement(918..2663)
                     /locus_tag="VMZ02_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUR72232.1"
                     /translation="MREKSQTLQQPVAFDAGWRASSFSYYLPSVLMTLLLVIVAFLVL
                     TPLFLMILNSFQTARPGQPIVWGFEGWVKAFTTPGIVKAMTNTITLAVTRQAIALLVG
                     AYFAWLIARTDIPMKGTLEFLFWLSFFLPALPQTMGWILLLDPKYGLFNQALHSIGIS
                     HALFNIYSFWGIIWAHLGGTIAVKVMLLAPAFRNLDAALEEASHISGASGRHTFFHIF
                     IPVMMPAILVSTILGVIRSLEAFEIELLLGTPIGLQVFSTKIHELVTWEPPQFAPAMA
                     LSTIFLGLLLFMVAMQRRYIGKRSYATVTGKSFSIRSMRLGPWRYPAFLLVMGFALLV
                     TVLPTILLVAGTFMKLFGFFNIPDPWTLDNWRATLGDPVLLRSLWNTIAVGLGSGVIG
                     IFFYALIAYIIVKTQFRGRWLLDFLSWLPWSIPGILLGVALLWTFLQTKIFLPIYGTV
                     YLLMVAMVIKSMPFGAQMIKSVLIQLGNDLEEASAVCGGTWLHTFRRVLLPLIMPAMI
                     TVALVGFISAARDISTVVLLGSGKSRTLSLLMLDFAAGAEFEKATVVAVIIVALVVGA
                     ALCARALGGQVGVRE"
     gene            complement(2713..3840)
                     /locus_tag="VMZ02_09610"
     CDS             complement(2713..3840)
                     /locus_tag="VMZ02_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72233.1"
                     /translation="MDSKLTTIHPSLRAKSAYERFVSAEGVPVIGGFGVHAVESVPMA
                     PWRRVGCDGAYFQFFGSDGILGLYAGKIPPRGVTLPERHLYEKMFYIFQGEGVGEFQQ
                     RDRVPQNILWRAGSLFSPPLNTLHRLINQSDQPALFLAVTTAPVAFDHYRNEQFIFHN
                     EFVFGERDDSAGYFQPDEDRFRIAHHREWVHQANFIPNVAEVGHRGAGGNRRRIKIAR
                     FEMAKNSLIGQLVEWPVGRYRKAHCRSGGVGLVILRSSGYSLMWPSHLGTQPYKHGLA
                     DQVVRLDWAPGSIFSPPPNWFHQHFNTGSEPAQTLAFFCGSEQFPLSRRTDGANAVAS
                     LGGTLRELHADDPSIRRMYEAELAKNGIAPSRNIARAASND"
     gene            complement(3830..5299)
                     /locus_tag="VMZ02_09615"
     CDS             complement(3830..5299)
                     /locus_tag="VMZ02_09615"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR72234.1"
                     /translation="MAFADLRDWIDDLEERRDLKRIGAEVDWNEELGAITREVSSQAG
                     PALLFENITGHRNTFCHRLFTNGTGTRERVCRLIGVPESTSYRDMVPIFKERFSRPVQ
                     PVTVASGPVKENILRGADVDLNQIPVPRWNPLDGGRYIMTSATVVTCDPDTGVLNGGT
                     YRGMITGKNTIGVLLAMTQGWGKHFSKYKARGEEMPVAVVIGWDQSLFLAASTPVNYP
                     EYEMAGSLRGAPVELVKCELSNLLVPASAELVLEGYVSPDSTTYTLEGPFSEYPGYYG
                     GSKSPKHTIRVECISHRNDPIFHGCLTGASPGRTNEGTTWTPATFSAMAWHYLEVAGV
                     PNITGVWRGKWPETMRVQIRKSHRGHAQQVANALWGCHLGNYAAKHVIVVDDDIDIHD
                     WEAIEWALCYRVNAGLGDVSFFAGTGGSMLDPSVPLEERNSVKYGHGSWTRMLIDATI
                     NWQLQPQEQYGGNRYPAVGTTVSPAMTERLKTRWREYGF"
     gene            complement(5362..>6045)
                     /locus_tag="VMZ02_09620"
     CDS             complement(5362..>6045)
                     /locus_tag="VMZ02_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72235.1"
                     /translation="NAKKYIYNFVAYQTVSLWSDANQYKPNEFKSFDDLLSPKLQGKI
                     GISDPRTPGSGSSMWSYMHYIKGEEWLKKFVAQKLFITRDLRLLAENLARGKIAVTSG
                     IGYSEFQPFIKANLPVTPLPVPKEGLYISGGYGHLTILKNNPHPNATKVFVNWLLSRD
                     GQETFGRGMGVGSRRLEIETKWLRDYGVIAAKDMMTPDQFYKYENQSEEKINKIREPA
                     AAFARKVLG"
BASE COUNT         1454 a         1718 c         1598 g         1275 t
ORIGIN      
        1 tgagaatcga cacgttcaac acgttgaggc ggtaggcctg ccagtaatac tgagacgcgg
       61 taaactgccg attctgagtc gagcaacgcc gcgcaagctt tcgatactta cgcgccagcc
      121 gcaggcgata gcgcaatgga ccgattgcac gcacgacttc gggataatgc gctcgaaggt
      181 cctcgaggca ttttacatga ccctccagtc ctacggtcgg caaaagattc tgcccggtga
      241 tctgttttac gcagccattg tagtcggcga agcgaacgat gtattggctg gccagccgca
      301 aataaaagtg taaatcgtct aatccccaaa tttttggatg caagccgcca agcttatcga
      361 aaatcgcttt tcgcaccatc accaccgaca tcgactctac acaacattgc cagatttgtc
      421 tgatgctcac cggcccgctg accaattctt tttcatgggc cggcgtgtga cgcggcgcga
      481 cattggtgtc ctccaagtcg cagtaagtcg ctttgtcgaa aaccattgca gtcgatggat
      541 ttttgaaaaa taccgccagc aatgtttcaa gatgttggtc attccagata tcgtcgtgat
      601 cgcagaatgc gataaattca ccctgcgcca aactgacgcc gcgatttcgt gcaaccgccg
      661 gcccttggtt ggtctggcga taaaatcgaa tgctcgagtc agctgatgtt acaagctttt
      721 gagtattgtc ggtcgatccg tcgtcgacga cgatgatttc gaaatcctga aaggtttgct
      781 gctgaatacc ggctaaagta tcgcgcaaaa gtggaccgct attaaaggca ggaataacaa
      841 tggaaacctt gggaaccacc atgctagtcg aagagttacg gaaaggagag atttaacgca
      901 agttattaag taaactctta ctctctgact cccacttggc cgccgagcgc acgggcgcac
      961 aacgccgcac cgacgaccag cgcgacgatg atgaccgcta ccactgtggc tttctcgaac
     1021 tctgcgccag cggcgaaatc aagcatgagc aacgataaag tgcgcgactt gcctgaaccg
     1081 agcagcacga ccgtactgat atcgcgagct gcggagataa acccaaccag cgcgacggta
     1141 atcatcgccg gcatgatcaa tggtagaagt acccggcgaa aggtatgtag ccaagtgccg
     1201 ccgcacactg cggacgcttc ttcgagatcg ttgcccagtt gaatcaacac actcttaatc
     1261 atctgagcac caaacggcat acttttaatc accatcgcca ccatcaagag ataaaccgtc
     1321 ccgtagatcg gcaagaaaat tttcgtttgc aggaaggtcc acaagagagc cacccccagg
     1381 agaattcccg gaatcgacca gggcagccag gaaagaaaat caagcagcca gcggccgcga
     1441 aattgtgttt tcacgataat gtaagcaatt aacgcgtaaa aaaatatgcc gatcacgccc
     1501 gaccccaaac ccaccgcgat ggtattccaa agcgaacgca gcagcacggg atcgccgagc
     1561 gtagcgcgcc aattatcgag cgtccacgga tccggaatat tgaaaaaccc gaaaagcttc
     1621 atgaaggtgc cggccactaa caagatcgtc ggcaagacag tgaccagcag cgcaaatccc
     1681 attaccaaaa ggaatgccgg ataacgccat ggcccgagcc gcatcgaacg aatgctgaaa
     1741 cttttgccgg tgaccgtcgc atagctgcgt ttgccgatat atcttcgctg catcgccacc
     1801 atgaacagca ataacccaag aaagatcgtg ctcagcgcca tcgccggcgc gaactgaggc
     1861 ggttcccagg taaccagttc atggatcttg gtcgagaaca cttgcagccc gatcggcgtt
     1921 cccagcagca attcgatttc gaatgcctcc aacgatcgaa tcacgcccag gatggtcgac
     1981 acgagaatcg ccggcatcat caccgggatg aagatgtgaa agaaagtatg cctgccgctg
     2041 gcaccggata tatgggatgc ctcttcgagg gcggcgtcga gattgcgaaa cgccggtgcc
     2101 aacagcatca ctttgacggc aatggtaccg ccgagatgcg cccagataat cccccaaaat
     2161 gaatagatgt tgaaaagcgc gtgactgatg ccgatcgagt gcagcgcttg attgaataga
     2221 ccgtacttcg ggtcgagcag cagaatccac cccatcgtct gcggtaaggc cgggagaaag
     2281 aatgacagcc agaacaaaaa ttccagcgtg cctttcattg gaatatcggt gcgcgcgatc
     2341 agccacgcga aataggcgcc aaccaaaagc gcgatggctt ggcgcgtaac tgcgagagta
     2401 atcgtgttgg tcatggcctt gacgattccc ggcgtggtaa acgctttgac ccaaccttcg
     2461 aagccccaga cgatcggctg gcccggccgt gcggtctgaa aactattgag gatcatcaag
     2521 aacaacggcg tcaacaccag aaacgcgacg atcacgagca acaaagtcat caggaccgac
     2581 ggcaaatagt aggaaaacga cgaggcgcgc catccggcgt cgaaagccac tggctgctgc
     2641 aaagtttgcg atttttccct catgttcaaa actaatgtgg tgccgtgttt atatgaagcc
     2701 gcaatccgat cgtcaatcgt tactggcagc ccgggcgatg ttgcggctcg gcgcgatgcc
     2761 gttttttgcc aactctgctt catacatgcg ccgaatcgac gggtcgtcgg cgtgaagctc
     2821 acgcaatgtc ccgcctaaac tggcgaccgc gttcgctcca tcggtgcgcc gactaagtgg
     2881 aaattgttcg ctaccgcaaa aaaaggccaa ggtctgggcc ggttcggaac cggtgttaaa
     2941 gtgttgatga aaccaattgg gcggtggact aaaaatactg ccgggcgccc aatccaagcg
     3001 cacgacttga tcggccaagc catgtttgta gggctgcgtg cccaagtggc tcggccacat
     3061 gagcgagtat cccgacgagc ggagaatcac caggccaacg ccgccactgc gacaatgcgc
     3121 ctttcgataa cgccctaccg gccactcgac cagttgacca atcaaactat tttttgccat
     3181 ttcgaagcgg gcaatcttaa tccgccgccg attgccgccg gcaccccgat ggcctacctc
     3241 ggccacattg ggaataaaat tagcctgatg aacccactcg cgatgatggg cgatccgaaa
     3301 tcggtcctca tcgggttgga aatagcccgc gctgtcatcg cgctcgccaa acacgaattc
     3361 attgtgaaag ataaattgct cgttgcgata atgatcgaac gccaccggcg ccgtcgtaac
     3421 agccaggaac agagccggct ggtccgattg attaatcagc cgatgcaaag tgtttagcgg
     3481 cggtgaaaat aggctgccgg cgcgccataa aatattctgc ggcacgcgat cgcgctgctg
     3541 aaactcgccg acgccctcgc cctgaaagat ataaaacatt ttttcgtaca gatggcgttc
     3601 cggcagcgtg acgccccggg gtgggatctt gccggcgtat aatcccaaga tgccgtccga
     3661 gccgaaaaac tggaaataag cgccatcgca accaacacga cgccaaggcg ccatcggtac
     3721 actttcgacg gcgtgaaccc cgaagccgcc aattaccggc acgccttcag cgctaacaaa
     3781 tcgctcatag gcgcttttcg ctctgagcga tggatgaatc gttgtcagct tagaatccat
     3841 actctctcca tcgcgtcttt agccgctcgg tcattgccgg agatacggtc gttcctaccg
     3901 ccggataacg gttgccgccg tattgctcct gcggttgcag ttgccagttg attgtcgcgt
     3961 cgatcaacat gcgcgtccac gagccatggc cgtatttaac cgaattgcgc tcctcgagag
     4021 gcacgcttgg atcgagcatg gatccgccgg tgccggcgaa aaacgaaacg tcacccaagc
     4081 cggcattaac ccggtaacac agcgcccact cgatcgcttc ccaatcatgg atatcgatat
     4141 cgtcgtcgac gacgatcacg tgcttggcgg catagttgcc gagatggcat ccccagagcg
     4201 cattggccac ttgctgggcg tgaccacggt gcgatttacg aatctgcact cgcatcgttt
     4261 ccggccattt gccgcgccac accccggtga tgttgggaac gcccgccact tctaggtagt
     4321 gccacgccat tgccgaaaag gtcgccgggg tccaggtagt accttcattg gttcgtcccg
     4381 ggctggcgcc ggtcagacag ccatgaaaga tcggatcatt gcgatggctg atgcattcga
     4441 cacggatggt gtgttttggc gacttgctgc cgccgtagta tccggggtat tcgctaaacg
     4501 gcccttcgag cgtataagtc gtggaatcgg gagacacgta cccttcgagc acaagctccg
     4561 cacttgccgg caccagtaaa ttgctcagct cacactttac taattccacc ggcgcgccac
     4621 gcagcgatcc ggccatctca tattcgggat aattgaccgg cgtagatgcg gccaaaaaca
     4681 agctctggtc ccacccgatc accaccgcca ccggcatttc ctcgccgcgc gctttgtatt
     4741 tgctgaagtg cttgccccag ccttgagtca tggccagcag aacgccgata gtattcttgc
     4801 cggtaatcat cccccggtac gtgccaccat tgagcacacc ggtatcggga tcgcaggtca
     4861 ccaccgttgc cgaagtcatg atgtagcgcc cgccgtccag aggattccat cgcggtacgg
     4921 gtatctgatt gagatcgaca tcagcgccgc gcaaaatatt ttctttaact ggaccgctag
     4981 cgacggttac cggctgcacc ggccgcgaaa agcgctcttt aaagatcggc accatgtcgc
     5041 ggtacgaggt cgattccggt acaccgatga ggcggcacac gcgctcgcgc gtccccgtgc
     5101 cgttggtaaa taagcgatgg cagaaagtgt tgcgatggcc ggtaatattt tcaaacaaaa
     5161 gagccggccc cgcctggctc gaaacttctc gggtgatggc tccgagttct tcgttccagt
     5221 caacttcggc gccgatgcgt tttaaatcgc ggcgctcttc aagatcgtct atccagtcgc
     5281 gcaaatctgc aaaagccact cgacctccgg tcatgaacat tcgaagacca atctttgatc
     5341 gctcgctttg agcgggcgga attagcccag cactttcctt gcaaaagccg ccgccggttc
     5401 gcgaatcttg ttgatctttt cttccgattg attttcgtat ttgtagaact gatcaggtgt
     5461 catcatgtcc ttggcggcga tgacgccgta gtcgcgcagc cacttggttt cgatctccaa
     5521 gcggcggctg ccgacgccca tgccgcggcc gaaggtttcc tggccgtcgc ggctgagcag
     5581 ccaattgaca aacactttgg tcgcgttcgg atgcgggttg tttttcagaa tcgtcagatg
     5641 gccgtagccg ccgctgatat acaacccttc tttcggcacc ggaagcggtg tgacgggcag
     5701 attggctttg atgaacggct gaaactccga atagccgatg ccggaagtga ctgcgatctt
     5761 gccgcgcgct agattttccg cgagcaagcg caaatcgcgg gtgataaaaa gtttctgagc
     5821 gacgaatttt ttcagccact cctcgccttt aatgtagtgc atgtaggacc acatcgacga
     5881 gcccgaaccc ggcgtgcgcg gatcgctgat gccgatcttg ccctgaagct tgggactcaa
     5941 cagatcgtcg aaggatttga actcattggg cttatactga ttggcatcgc tccataggct
     6001 tacggtctga taagcgacga agttgtagat gtatttcttg gcatt
//
LOCUS       DATLCH010000325         3714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7891928, whole genome shotgun sequence.
ACCESSION   DATLCH010000325 DATLCH010000000
VERSION     DATLCH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7891928"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VMZ02_09625"
     CDS             <1..656
                     /locus_tag="VMZ02_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HUR72236.1"
                     /translation="TGVAVKETKLFAAGEILPEFGQNQDIYKIAFGLGSKDISQIVEA
                     NNAYLFLRIKQRKEAAVPPLESVKDQVEKGAKEAKAYELTLQKGNSVLEQLKKEKDFS
                     KIAAANDVQIEESGWFQRNAPQLPKIGDLSELRGGALLLSAQNPVADKLYTQKDAAYI
                     LAFKASQPADMEQFEKEKALLKKQATVESRQRALAKYLESLKAKAKITFNNDFLEAS"
     gene            676..1308
                     /locus_tag="VMZ02_09630"
     CDS             676..1308
                     /locus_tag="VMZ02_09630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HUR72237.1"
                     /translation="MQIVLVRHGATDWNLEHRCQGSSDRDLSDVGRRQAEQLGERLRQ
                     ETIHGVYASDLRRARRTAEVISRHHNLPVIVEESIRELDHGHLEGLTFNEIKQNYGDF
                     LTRWRSEPAELRVPGGECLADVAERAWLGLNQIVARHDAAETIVVVSHNFPIVGIVCR
                     ITGTHLNQYRNFHLDPCGMSRLHHNGDGSWQVTHINGDEYEKHRGRAIPA"
     gene            complement(1332..2870)
                     /locus_tag="VMZ02_09635"
     CDS             complement(1332..2870)
                     /locus_tag="VMZ02_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72238.1"
                     /translation="MTHPASNPPWFVRKDLDGFFGLMIDNLIQLILIVGLCRELILLP
                     DSYIFGRILPGAAISILLGNFFYAWQARRLAQESGREDVTALPYGINTVSLFAFVFFI
                     MLPIVQETKDPVWAWKVGLVACFLNGVIEILGAFVAEAVRRVTPRAALLSALSGIAIT
                     FIAMDFTFKIFAQPLVALVPMALIFVAYFAHQKMPLGLPGGLVAIAVGTILGWTMGSM
                     NSKALTLHGSLSLPVFTGDSLWEALVHPAFLNYFSVIFPMGIFNVVGSLQNIESAEAA
                     GDRYKTFPSLMANGIGSVVASLFGSVFPTTIYIGHPGWKRLGARSGYSSLNGIFIALL
                     CFTGTIQAVLGLIPLEAGIGILLYIGIIIVAQSFQETPSEHAPAVALGLVPALAGWGL
                     MMVETGLRAAGKSLFDVGLAKFSHESVAIHGMISLERGFIFTSMILAAVGVALIERKF
                     ARAGLWSVTAAVLSACGIIHAYELSAGGITSRFGLMAAPEFFLAYLAMAIIFFIIGWL
                     DKRE"
     gene            2937..3686
                     /gene="lipB"
                     /locus_tag="VMZ02_09640"
     CDS             2937..3686
                     /gene="lipB"
                     /locus_tag="VMZ02_09640"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HUR72239.1"
                     /translation="MANLALKPRLGEDNSEIQSMSTLRVKNCRTLSLLAAIALQEKLV
                     ALKQQGSRADILLFVEHPHVYTLGRGGSDVNVLAPKEVPVHRTSRGGDVTYHGPGQLI
                     VYPIIDLRSKLRKDVHRYVRNLEQSAIQTLADFAIVAGRRPPHTGIWIDDRKIAAIGI
                     AVRRCITYHGLALNVNTDLSFFQRIVPCGLAWAEVTSMRKELGVEPRMTDVRQRFLHH
                     FAEIFGYHDIDEGIEQSGDLNVLNGLNVLNS"
BASE COUNT          980 a         1030 c         1000 g          704 t
ORIGIN      
        1 aaaccggcgt agcggtaaaa gaaaccaaat tattcgcggc gggggaaatt ctcccggagt
       61 ttggccagaa ccaggatatt tacaaaatcg cctttggcct cgggtccaaa gatatcagcc
      121 agatcgtcga ggctaataac gcgtaccttt ttctccgcat caagcaacgc aaagaagccg
      181 cggtaccgcc cctggaaagt gttaaggatc aagtcgaaaa aggcgccaaa gaagccaagg
      241 cgtacgagct aacattacaa aaaggcaata gcgtgcttga acagctgaag aaagaaaaag
      301 atttcagcaa aatcgccgca gctaacgatg tgcagatcga agagagcggc tggtttcaac
      361 gcaacgctcc ccagctgccg aaaatcggcg atctctccga attgcgcggc ggcgctctct
      421 tgctatcagc gcagaatcca gtcgcggaca agctttacac gcaaaaagat gccgcctaca
      481 ttttagcgtt taaagcgagt caacctgccg acatggagca gttcgaaaag gagaaggctc
      541 tgttgaaaaa acaagccacc gtcgagagcc gccagcgtgc cttggcgaag tatctcgaaa
      601 gtttaaaagc caaggcaaaa atcaccttca acaacgactt cttagaagcg agctgagccg
      661 gcccggaatg ctgacgtgca aatagttctc gtgcgccatg gcgccaccga ttggaacttg
      721 gagcatcgct gccagggatc gagcgatcgc gatctgagtg atgtcggacg gcgtcaagcc
      781 gaacaacttg gcgaacggct gcgtcaggaa acgattcacg gggtctatgc gagtgatttg
      841 agacgcgcac ggcgcactgc tgaagttatc agccgacatc acaatctacc ggtaatcgtc
      901 gaagagagca ttcgcgaact cgatcatggc catttagaag ggctgacgtt taacgaaatc
      961 aaacagaact atggcgattt tctgacgcgc tggcgcagcg aaccggccga gttgcgagtc
     1021 cccggcggcg aatgcctagc ggatgtcgcc gagcgcgcct ggttgggcct aaatcagatc
     1081 gtcgcgcgcc atgacgcagc ggagaccatc gtcgtcgtca gccacaactt cccgatcgtc
     1141 gggatcgtct gccgaattac cggaacccac ctgaaccaat accgaaattt tcacctggac
     1201 ccgtgcggca tgagccggtt acaccataac ggcgacggct cctggcaagt gacccatatc
     1261 aatggtgatg agtacgaaaa gcaccgcggc cgggcaatcc cagcctgaca ctttatccaa
     1321 cgtgccgcgg ctcactcgcg cttgtcgagc caaccgataa tgaaaaaaat gatcgccatt
     1381 gcgagatagg cgaggaaaaa ttccggtgcc gccatcaatc caaaccggct ggtaatgcca
     1441 cctgctgaga gttcgtaggc atggatgatg ccgcaggcag aaagcactgc cgcggtgacc
     1501 gaccacaacc cggctcgggc aaattttctt tcaatcagcg ccactccaac ggcagcaagg
     1561 atcatcgagg tgaaaataaa gccgcgttca agtgatatca ttccatgaat tgccacgctc
     1621 tcatggctga atttagccaa gccaacgtcg aagagggatt tccccgccgc ccgtaatccc
     1681 gtttcaacca tcatcaatcc ccatcccgcc aacgccggca ctaatccgag cgccaccgcc
     1741 ggcgcgtgct cgcttggcgt ttcttgaaac gattgggcga cgatgatgat gccaatgtaa
     1801 aggagaatgc ctatacccgc ttccagggga ataagaccga gcaccgcttg gatagtgccg
     1861 gtgaaacaga gaagcgcgat aaaaatccca ttgagcgaag aatagcctga gcgcgcgccg
     1921 agccgtttcc accccggatg cccaatataa attgtcgtcg gaaacacact gccgaacagc
     1981 gacgccacga ccgagccgat gccgttggcc atcagtgagg gaaaggtttt gtaacggtcg
     2041 ccagccgctt cggcgctctc gatgttttgc aaggagccga ctacgttaaa aatccccatg
     2101 ggaaaaatca ccgaaaaata attgagaaag gcgggatgaa cgagcgcttc ccagagcgag
     2161 tcaccggtaa acaccggcag ggacaaagat ccatgcagcg tcagagcctt gctgttcata
     2221 gaccccatcg tccagccaag aatcgtaccg accgcaatgg caaccaggcc gccgggcagg
     2281 cccaacggca ttttctgatg cgcaaagtag gcgacgaaga tcagcgccat tggcaccagc
     2341 gccacgagcg gctgagcgaa aattttaaaa gtaaaatcca tcgcgataaa agtgatcgcg
     2401 atgccggaca aagccgaaag caaggcggcc cgcggcgtca cccgacgcac cgcctcggca
     2461 acgaaggcgc cgagtatttc gatcacgccg ttgagaaaac acgcgaccag gccaaccttc
     2521 caggcccaca ccgggtcttt ggtctcttgc acgatcggca gcatgataaa aaagacgaaa
     2581 gcgaacagac tcacggtatt gataccgtaa ggtaacgcgg tgacgtcttc gcggccgctc
     2641 tcctgggcca gccgacgcgc ctgccaggcg taaaaaaaat tgcccagcag aatcgaaatc
     2701 gccgcgcccg gaaggattct gccgaaaata taactatccg gcagcagaat cagctcgcgg
     2761 cacaggccga caatcaaaat gagctggatg agattgtcga tcatcaaacc gaaaaatccg
     2821 tcgagatctt tgcggacaaa ccagggtgga ttcgatgccg ggtgcgtcat gtgcttagaa
     2881 actgtcgctg caagtcggcc gactgtagcc gtcgatactc gaggatgtca aatcatatgg
     2941 caaacctcgc cttgaaaccc cgccttgggg aagataatag tgaaatccaa tcgatgtcga
     3001 ctttacgggt caaaaattgt cggacgttga gtctcttggc agccatcgca ttgcaggaga
     3061 agctggtggc gctgaagcag caaggctcac gcgcggatat cctgcttttc gtcgagcatc
     3121 cccatgttta taccctcggt cgcggcggta gtgacgtcaa cgtgctcgcg ccgaaggaag
     3181 ttcccgtcca tcgcaccagc cgcggcggcg atgtcacata ccacggcccg ggtcaattaa
     3241 ttgtttatcc gatcatcgat ttgcgctcga aattacgcaa agacgtgcat cgctacgtgc
     3301 gcaatctgga acaaagtgcg attcagacat tggcagactt cgccattgtc gcaggacgcc
     3361 ggccaccgca cacggggatt tggattgacg acagaaaaat cgccgccatt ggcatcgccg
     3421 tgcggcgttg cattacctac cacggcttgg cgctcaacgt gaacaccgac ttgtcgtttt
     3481 tccagcgcat tgttccgtgc ggactggctt gggctgaagt gacctccatg cgaaaggaac
     3541 tcggtgtgga gccgcgcatg actgatgttc gacagcgatt tctccatcac ttcgccgaaa
     3601 tctttggtta tcacgatata gacgaaggga ttgagcagtc cggcgatttg aacgtattga
     3661 acggcttgaa cgttttgaac tcgtgactct tatgggtcga agacatcccg attg
//
LOCUS       DATLCH010000326         4286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6409115, whole genome shotgun sequence.
ACCESSION   DATLCH010000326 DATLCH010000000
VERSION     DATLCH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6409115"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VMZ02_09645"
     CDS             <1..528
                     /locus_tag="VMZ02_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR72240.1"
                     /translation="AVDYLKKLPQVDASRIAIIGVCQTGRQPVLLAAKRNDIAGAVVL
                     YGAIGGREWQASDSRPTPIEDLIAEVSCPVLGIFGEADHIISVDDVARFRNCLEKAKK
                     SCHIRIYRDAPHGWLNDTMPGRYRKEAANDAWKLMIGFLKKCFAGAWDSQRISCTYES
                     DYAAAYDFKNNVRME"
     gene            555..1511
                     /locus_tag="VMZ02_09650"
     CDS             555..1511
                     /locus_tag="VMZ02_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72241.1"
                     /translation="MKTEKTFRQFALLVCLVGSVAIGPWPASAERVSIATPSRGLFEF
                     PVVVAMRKGFYKAEGIDVDKVQMQPTLGVQALVAGDVDYLLAWGSALRAAVTGVPIKN
                     VVGIAGRPLHVLIARAGIQNPKELRNKVIGVDSVAGTVDYLSRVAVRHFGMEPDKDVK
                     IIVTGESPTRLAALRSGAIDATPIDVAFAVKAEDEGFRRMVYLGDLIELPLSGMAVTD
                     HKLQTQRDQVRRMVRATVRGTRSFKQNRTETVQMLADYLRITPAQAAKAYDTSLNSFS
                     DDGMISDKGVLLDVQLTKERLKMTKDVPLSQLVDWSLVREIK"
     gene            1825..2667
                     /locus_tag="VMZ02_09655"
     CDS             1825..2667
                     /locus_tag="VMZ02_09655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HUR72242.1"
                     /translation="MQHSAECLAPDRLFSPNSELWRVDREMALLMAGGRALLMQLAHP
                     MVAGGVAQHSDFKADPLGRLQRTMNAMWTIGFDERSRAEAALQRVNRIHEGVRGIVPA
                     SEPGLPAREYFALDQELLLWVHATLIDSALAAYQAFVAPLSTAAKRRYYDDSKRLAEL
                     FKIRREIVPASLSEFEAYMRRMVAGGEITVGPTARILARDILYPRPWLLRPAGPLFRL
                     VSAGLLPRELRRGYGFTWNERRERRLRLFTRVVRRFLPLLPAPLRIVPQARRAEREIH
                     RLAR"
     gene            2787..3428
                     /locus_tag="VMZ02_09660"
     CDS             2787..3428
                     /locus_tag="VMZ02_09660"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HUR72243.1"
                     /translation="MSTNSSRLESSDYFIRRTIRLKLPRSFYQQSTLVVAKQLLGKFL
                     VHRHVTGTLIGRIVETEAYLGPHDLACHAAKGRTRRTEVMFGAAGHAYVYFIYGFYNM
                     LNLVTEAKGYPAAVLIRALEPIYGLEQMKHNRKTDVVAKLASGPGKLCQAFAIDRSLN
                     GEDLCGKVLYLEDRGEATPEFDATPRIGVDYAGKWKDELLRFSVRDSKFVSKR"
     gene            3500..4243
                     /locus_tag="VMZ02_09665"
     CDS             3500..4243
                     /locus_tag="VMZ02_09665"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HUR72244.1"
                     /translation="MEQRVIAEVNRRLASLEQSEDMAILLSVESGSRAWGFESTDSDY
                     DVRFIYIRRPDAYLSIDLETHPDVIEQPIVDDIDISGWDIRKALKLFQKSNPPLLEWL
                     QSPLIYIERHSFAAKLRALLPDFYSPRNCFHHYFHMARGNFREYLRGDTVRRKKYFYV
                     LRPLLALRWLELDLGPVPMEFLKLVDRTITDTPLRQAIDDLVIAKRAGVEMDDGPPIS
                     IISDFIEREMPRLKASLPICQNKSLRLRH"
BASE COUNT          925 a         1159 c         1225 g          977 t
ORIGIN      
        1 gcggtggatt acctgaaaaa actgccccaa gtcgacgcct cgcgcatcgc cattatcggc
       61 gtgtgccaga cgggccgcca gccggttttg ctggccgcca agcgtaacga tatcgccggt
      121 gccgtggtcc tctatggcgc catcggcggg cgagagtggc aagccagcga ttcgcggcca
      181 acgccgatcg aggatttaat cgccgaagtc agctgtccgg tgttgggaat ttttggtgag
      241 gcggatcaca tcatatcggt cgacgacgtc gcgcgcttcc gcaattgttt ggagaaagcg
      301 aaaaagagct gtcatattcg gatttaccgc gacgcacccc atggttggct taacgacacg
      361 atgcccggcc gctaccgtaa ggaggccgcc aacgacgctt ggaagctgat gatcgggttt
      421 ctcaagaagt gctttgccgg ggcatgggat tcccagcgta tctcttgcac gtatgaaagt
      481 gactacgccg cggcgtatga cttcaaaaac aatgtgcgta tggaatagca agcgacggga
      541 gagaattgcc gccaatgaaa accgaaaaaa ctttccggca gttcgcgttg ctggtttgtc
      601 tcgtgggatc ggttgcgatc ggtccctggc cggcatcagc cgaacgagtc tcgatagcga
      661 cgccgagccg agggctgttc gagtttcccg tcgtggtcgc catgcgcaaa gggttttaca
      721 aggctgaagg catcgatgta gacaaggtgc agatgcagcc gacgttgggc gttcaggcgc
      781 tggttgccgg cgacgtcgat tatttgttgg cgtggggctc ggcattgcgc gcggcagtta
      841 ccggtgtgcc gattaaaaac gtcgtcggca tcgccggccg gccgttgcat gtattaatcg
      901 cccgcgccgg cattcaaaat cctaaagaac tgcgcaacaa ggtcatcggt gtcgactcgg
      961 tggcgggcac ggttgattac ctgtcgcgag tcgcggtgcg tcactttggc atggagccgg
     1021 acaaggatgt gaaaatcatc gtcaccggcg agagcccgac gcgtctggcc gccctgcgca
     1081 gcggcgccat cgacgcaact cccatcgatg ttgcgtttgc agtgaaagcc gaagacgaag
     1141 gttttcgccg catggtctac ctcggcgatc ttatagaatt gcctctttcg ggaatggccg
     1201 tcaccgatca caaactgcaa actcaacgcg atcaagtacg tcgcatggtg cgagcgacgg
     1261 tgcgcggcac gcgttctttc aaacaaaacc gcaccgagac ggtgcagatg ctcgccgact
     1321 atctgcgcat cacaccggca caagccgcca aagcgtacga cacctcgctc aactccttct
     1381 cggacgacgg catgatctcc gacaagggcg ttctcctcga cgtccaactc accaaagagc
     1441 ggctcaagat gaccaaggat gttccgttga gtcagttggt ggattggagt ttggtgagag
     1501 aaattaagtg acgcaagtga atcaactcac gcaaatggcc gggtgagaaa tcgtctgcaa
     1561 acgattcgta aaaatataga gcaactacgc tagagttcga ccggcctaat tctaccggtt
     1621 tcggtgtgca ccaacctcca gcacgcctgt tcagcctgct tgctgatcct ggctctgcga
     1681 catcgccccg gagtaaagtt acaattttgc ggcagcggat tctcatagga gcatttacga
     1741 ttttcaaagg cgcgatgttg aaccgattcg tagccgaacg tgaatcgtat tgtctcgaca
     1801 gttccggctg tctaagctgt ttctatgcag cactcggccg aatgcttagc tcctgacagg
     1861 ctgttttcgc ccaatagcga gctttggcgc gtcgatcgtg agatggcatt gttgatggcc
     1921 ggcggccgcg cactgctgat gcaactcgcc catccgatgg ttgccggcgg ggtggctcag
     1981 cacagcgact ttaaagctga tcctctcggc cgattacaac gaaccatgaa tgccatgtgg
     2041 acgattggct tcgacgaacg gagccgagcc gaggctgcac tgcagcgtgt taaccgtatc
     2101 catgaagggg ttcgcggcat tgtgcctgcg agcgagccgg gattacctgc aagagaatac
     2161 ttcgcgcttg atcaagagtt gctgctatgg gtacacgcca cgctgatcga ttccgcactg
     2221 gcggcttacc aggcgtttgt cgcgcccctg agcactgcag ccaagcgccg ttattacgac
     2281 gattcaaagc ggcttgccga gctcttcaaa atacgccggg agattgttcc cgcgtccctg
     2341 tcggaattcg aagcctacat gcggcggatg gttgccggcg gagaaataac cgtcggtcca
     2401 acggcgcgga tactggcccg ggacattctc tatccgcggc cctggctgct gcgaccggcg
     2461 gggccactgt ttcgcctggt gagtgcgggt ttgttgcccc gtgaattgcg ccgtgggtac
     2521 ggttttacat ggaacgagcg gcgcgaacgg cgccttcgcc tgttcactcg cgtggtccgg
     2581 cgatttttac cgctgcttcc ggcgccgctg cgcatcgtac cgcaggcgcg tcgggcggaa
     2641 cgggaaatac accgcttagc tcggtaaacc ggcgtgcatg gagtaaattc aattggctcg
     2701 ccgctatggc gagattcccc atgacgcatg atgcgcaatc gtcgcgtggc aaagcgaagg
     2761 ttgatttcct cggccggttg cctaccatgt ctacaaattc ttcgcggctc gaaagcagcg
     2821 actacttcat tcggcgcacg attcgcttga agcttcctcg gtcattttac caacaatcga
     2881 ctctcgtggt tgccaagcaa ttgctcggga aatttcttgt ccatcggcat gtgacaggca
     2941 cattgatcgg ccggatcgtc gagacagaag cctatcttgg cccacacgat cttgcctgtc
     3001 atgcggctaa gggaagaacc aggcggaccg aggtgatgtt tggcgcggcc ggtcacgcct
     3061 atgtttactt tatctacggc ttctacaaca tgctcaatct ggtcaccgag gcaaagggtt
     3121 atcccgcggc ggtattgatc cgcgcgctgg aaccgattta cggcctcgaa cagatgaaac
     3181 acaaccgcaa aaccgacgtg gtcgctaaac tcgccagcgg tccgggtaaa ctttgccagg
     3241 cgtttgccat cgaccgttcg ctcaatggcg aggatctctg tggtaaggta ctttatctcg
     3301 aagaccgcgg tgaagcgacg cccgagttcg acgccacccc gcgcatcggt gtcgattatg
     3361 ccggcaagtg gaaggatgag ctgttacggt tttcggtgcg cgacagcaag tttgtttcga
     3421 agcgctgatg ctaaactttt tggcttagcg aagcgacttg cacaatgtag aatgacaact
     3481 gcggatttca gcctagtaaa tggaacaacg agtcatcgca gaggttaatc gtcgccttgc
     3541 gtcattagaa caatccgaag acatggcgat ccttctgtct gtcgagtccg gcagccgggc
     3601 ttggggattt gagtccaccg attcggatta tgacgtcaga tttatctata tcaggcggcc
     3661 tgatgcttac ttgtccatcg acctcgaaac tcatccagat gtgatcgagc agcccatcgt
     3721 cgatgacatc gatattagcg gttgggacat tcgtaaggcg ctgaagttgt ttcaaaagtc
     3781 gaatccacca ttgctcgaat ggctgcaatc accgctgatc tatatcgagc gtcattcctt
     3841 cgcggcaaag ttgcgcgctt tgttgcctga tttttattcg ccgcgcaact gttttcacca
     3901 ctactttcac atggcacggg gcaatttccg cgaatattta cgaggcgata ccgtccggcg
     3961 caaaaaatat ttttacgtac tacgaccgct attagcgttg cgctggctag agcttgacct
     4021 gggaccggta ccgatggagt ttctcaagtt agtggatcga accattaccg atacaccgct
     4081 gcgtcaagcc attgacgatt tggttatagc caagcgcgcc ggagtagaga tggatgatgg
     4141 accacccata tcgattatta gtgactttat cgaacgggag atgccacgtt tgaaagcctc
     4201 gctgccaata tgccaaaaca agtcacttcg attacggcat tgaatcagct gtttcgcgcg
     4261 actttggacg aagtctggca tggcta
//
LOCUS       DATLCH010000327         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7245107, whole genome shotgun sequence.
ACCESSION   DATLCH010000327 DATLCH010000000
VERSION     DATLCH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7245107"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..733
                     /locus_tag="VMZ02_09670"
     CDS             134..733
                     /locus_tag="VMZ02_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HUR72245.1"
                     /translation="MRKLTFRTAYLIPLGMLAVMTASGCGYNDLQGVDEEVKGAWAEV
                     QNQYQRRADLIPNLVATVKGAANFEQETLQKVIEARSAATSIKLDAQALSDPAMFKKF
                     EDAQRNLSGALSRLLVVAEQYPDLKANQNFRDLQAQLEGTENRVAVARGRYSKAVVEY
                     NKKVRFFPTNITAQYLLGMKVRENFSADEAAQKPPQVKF"
     gene            787..1587
                     /locus_tag="VMZ02_09675"
     CDS             787..1587
                     /locus_tag="VMZ02_09675"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HUR72246.1"
                     /translation="MTNKWRCGAVMPLYRRTLIIILLLIGSAAALDVPPLRGRINDYA
                     NVLQTGQVAALESQLAQLERDTGHQVAVLTVPTLDGEDIEGFSIRVAENWKIGKKGYD
                     NGVILVVAVKDRRLRLEVGYGLEGILPDAIAKRITADYIVPYFRQQDYGGGIVAGIAA
                     VDKIIRKEPLPESARKKIQNPGGHLSFPVMLAITFAVLGLMAFSANSNRQRSGMWATR
                     GRRYGPTVFGGPGGWGGGGGFSGGGGFGGGGFGGGGGGFGGGGSSDSW"
     gene            1590..2216
                     /locus_tag="VMZ02_09680"
     CDS             1590..2216
                     /locus_tag="VMZ02_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72247.1"
                     /translation="MKAENFFSEKEKERIREAVVAAESKTSGEVVPMIVTSAARYTEI
                     ELFGLAAGLFLGMVIEWLWGDPWGSPYFQLWPMFGALLGFLLGRIPGVKRLLAPTSRV
                     SEAVHTLGLASFTEQGLHYTKDHTGILILVSLLEHRVEVLADRGIDQKVGAGTWDEIV
                     HIISAGIKSGRACDAFCNAIRRCGDILATHFPRQADDQDELSNRLVTK"
     gene            2265..>3056
                     /locus_tag="VMZ02_09685"
     CDS             2265..>3056
                     /locus_tag="VMZ02_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72248.1"
                     /translation="MRKRLLITLSAMMLLATARPCLAQEKLNFAYISPNAGSSSVLWI
                     AKDAGIFKKHGLDVNVIYIEGTPKALMSLFAGELHIVAGTGPAVASAKVRGADVSMIM
                     GFEVFLPYYLVAQPNIKTVEELKGKIGANHTAATSADFAIRLGLKSIGLEPDKDVNLR
                     VVGATNLRLIMMQQKQADFTIISTTEREEAEKMGLKVIADLASKKIPYPHSGLISSQK
                     LLKERRDTMLRFGRATVEWIHFFKNNKAQTLAILKKYAKTDSTTLD"
BASE COUNT          731 a          847 c          834 g          644 t
ORIGIN      
        1 aacaaaccaa gtacttggag aatcgtagca aattacaatt cggaccactt gtcaaaacaa
       61 ttgccaattc ttactttata cggcaatact cggtgaacct gaactaatcc actcattcga
      121 ctaggagaga cttatgcgca agttaacctt ccggaccgct tacctcattc ctctaggcat
      181 gctcgccgta atgactgcgt ccggctgcgg ctataacgac ctccagggag tcgatgaaga
      241 agtcaaaggc gcctgggccg aggtgcagaa tcagtaccag cgccgtgccg acctgattcc
      301 aaatttggtt gcgactgtaa agggcgcggc taacttcgaa caagaaactc tgcaaaaagt
      361 cattgaagcg cgcagtgctg cgacctcgat aaaacttgac gcgcaagcgc tcagcgatcc
      421 ggcgatgttc aaaaaattcg aggatgccca acgcaatctc tcaggggcgc tgtcgcgact
      481 cctggtcgtc gccgaacaat atccggatct caaagctaat cagaacttcc gcgaccttca
      541 agctcagctc gaaggcacgg aaaaccgtgt cgccgttgcc cgcggccgct acagtaaggc
      601 ggtggtagag tataacaaaa aggttcgctt tttcccgacc aatataaccg ctcagtatct
      661 gctcggtatg aaagttcgtg aaaacttcag cgccgacgaa gccgcgcaaa agccgccgca
      721 ggtgaaattt taatcgggct gttcgagaag aactcgaggt aatacactct gaaggccctt
      781 cttggtttga ctaacaaatg gcgatgcggc gcagtgatgc cgctgtatcg ccggaccttg
      841 atcataattc tattactgat tggctcggca gcagcacttg atgtgccgcc gttgcgcggc
      901 cggatcaacg actacgccaa cgtgctgcag accggtcaag tcgcagcgtt ggaatcgcaa
      961 ctcgctcaac tcgaacgcga caccggccat caggttgcgg tgttaactgt cccgacgctc
     1021 gacggcgaag acatcgaagg cttcagcatc cgtgtcgcgg agaactggaa gatcggcaag
     1081 aaaggctatg ataatggcgt cattctagtc gttgcggtca aggatcgcag actccgtctc
     1141 gaagtcggct acggcctcga gggcatactg ccggacgcga tcgctaaacg catcacggct
     1201 gactacatcg tgccttactt ccgtcagcag gattacggcg gcggtattgt cgccgggatc
     1261 gccgccgtcg acaaaattat tcgcaaagaa ccgttgcctg aatcggcgcg caaaaaaatc
     1321 caaaatccgg gcggccactt aagctttcct gtcatgctcg ccatcacttt tgcggtttta
     1381 ggtttgatgg cgttctctgc aaactcgaac cgtcaacgca gcggcatgtg ggcgacgcgc
     1441 ggccggcgtt acggacccac tgtcttcggc gggccgggcg ggtggggcgg aggcggtggc
     1501 ttttcaggag gcgggggttt tggcgggggt ggttttggcg gtggcggagg tggtttcggc
     1561 ggcggcggct catccgatag ctggtagcaa tgaaagctga aaatttcttt agcgaaaaag
     1621 aaaaagagcg cattcgcgag gcggtagtcg cggccgagtc gaagacttcc ggtgaggtcg
     1681 tgccgatgat tgttacatcg gcggcgcgct acaccgagat cgaactgttc ggtctagccg
     1741 cgggactctt cctcggcatg gtcatcgaat ggttatgggg cgatccctgg gggtcgcctt
     1801 actttcaact gtggccgatg ttcggcgcac tgctcggttt cttactcggc cggataccgg
     1861 gtgtaaaacg actcctggcg ccgacaagcc gcgtcagcga agcggtgcac acgcttggcc
     1921 tcgcctcatt taccgagcaa ggcctgcact acaccaaaga ccacactgga atcttgatcc
     1981 tggtttctct cttggaacat cgtgtcgaag ttcttgccga ccgcggtatc gatcaaaaag
     2041 tcggcgccgg cacctgggac gaaatcgtcc acataatttc agctgggatc aaatccggcc
     2101 gagcctgcga tgccttctgt aatgcaattc ggcgttgcgg cgatattctg gcgactcatt
     2161 ttccgcgcca ggcagatgac caagacgagc tatccaatcg cctggtcacc aagtagcgca
     2221 aatcattgct atctcccgcc actcagcgaa aggacgcaaa cgccatgcga aaacgattgt
     2281 tgataacact cagtgccatg atgctcttag caactgctcg gccgtgtcta gcgcaagaaa
     2341 aacttaattt cgcatatatt tcgcctaacg cgggttcttc gtcggtgctt tggatcgcca
     2401 aggacgccgg catcttcaag aagcacgggc tcgacgttaa cgtgatctac atcgaaggca
     2461 cgccgaaagc tctcatgtcg ctgttcgccg gcgaacttca tatcgtcgcg ggcaccggcc
     2521 cggcggtcgc cagcgccaaa gtgcggggcg ccgacgtgag catgatcatg ggcttcgaag
     2581 tttttctacc ttattactta gtcgcgcaac cgaacatcaa aacggtcgaa gagctaaaag
     2641 gtaaaattgg cgccaaccac accgcggcga cgtcagcgga cttcgccatt cgcctgggtc
     2701 ttaaaagcat cggtctcgag ccagacaagg atgtcaacct gcgtgtcgtc ggcgcaacca
     2761 atctccgttt gatcatgatg cagcagaaac aagccgattt cacaattatt agtaccactg
     2821 agcgcgagga agcagagaag atgggactca aggtcatcgc cgatctcgcc agcaaaaaaa
     2881 ttccctaccc gcacagcggc ttgatcagta gccagaagct gctcaaagaa cgacgcgata
     2941 ccatgctgcg cttcggccgc gccaccgtcg agtggatcca ttttttcaag aacaacaaag
     3001 cgcagactct ggcgattcta aagaaatacg ccaaaaccga tagcaccacg ctcgac
//
LOCUS       DATLCH010000328         3201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7906139, whole genome shotgun sequence.
ACCESSION   DATLCH010000328 DATLCH010000000
VERSION     DATLCH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7906139"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VMZ02_09690"
     CDS             <1..438
                     /locus_tag="VMZ02_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72249.1"
                     /translation="WTVPDGRVWRDLKTLDFRLRQGNNIALWIRWDELSNTFSLCDRS
                     IKKSKGRRGGGLPGSPITCGAGELPGSPLVLEGALGQLHLAETTVIGSGPTGQSVTLN
                     LAIELGPNATGHYAVELAATDDFGAEDDFVQASDVLIRPAKRR"
     gene            complement(585..728)
                     /locus_tag="VMZ02_09695"
     CDS             complement(585..728)
                     /locus_tag="VMZ02_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72250.1"
                     /translation="MKELALEQVRSSSSVWAGDARAVARVRALRSVKNLIDASRESWN
                     WTR"
     gene            complement(829..1515)
                     /locus_tag="VMZ02_09700"
     CDS             complement(829..1515)
                     /locus_tag="VMZ02_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HUR72251.1"
                     /translation="MLDQNLIQIAGIIDAGEAQMLQQCGVRYLGFPLRLPVHREDLTE
                     QEAAAIIKVLTPPVFGVAITYLNQATEIAAFCHALGARIVQLHGDIERDELKRLKTLD
                     PNLTVLKSLVIGMREQKALEATLRELSPFVDGFITDTFDPETGASGATGKTHDWRVSR
                     RLVELADRPIILAGGLTPENVKSAILEVGPAGVDSHTGVEDSTGRKSREKVQKFVSEA
                     NEAFDLVDRR"
     gene            complement(1579..2121)
                     /locus_tag="VMZ02_09705"
     CDS             complement(1579..2121)
                     /locus_tag="VMZ02_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HUR72252.1"
                     /translation="MARKTSAGLLLYRRTPEVEVFLVHPGGPFWRKKDAGAWSIPKGE
                     FAAGEELLQAAQREFTEETGFTISGEFRCLDPLKQPGGKIVYAWAIEADCDPAQIRSN
                     SFSLEWPPKSGNMQQYPEVDRAAWFNISEAREKILSGQVGFIEQLIEDLGRRKSESQP
                     AKCEVPGGSLSKSANGKRGR"
     gene            complement(2369..>3201)
                     /locus_tag="VMZ02_09710"
     CDS             complement(2369..>3201)
                     /locus_tag="VMZ02_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72253.1"
                     /translation="LGGKHEEKRPLRQLSALPVKRYVFINRDSHPDADIYVAIHEAKD
                     LPAQVADYQVPHCHNTDEFYYFIGNNPDLTGLEGQIIFDGKVHKIVAPACVYIPTGAV
                     HEYKVTRGAGTVTVLFRNRGYTHEDKPFDVAKGEREFSKYAGYIFPPEVRPTTEIKYH
                     TDAAPGTRYVFVDGKVKPEANFYTVVRSVAGVQPTQADYVDMHRHNCDTQHIAIGTGA
                     ELTGLKIEFQIGDDKVVVESPVGVNIPAGTPHSQRIIEGAGHFFNFVPKSFYNDGLMS
                     "
BASE COUNT          681 a          913 c          881 g          726 t
ORIGIN      
        1 tggactgtgc cggatggccg cgtatggcgt gatctaaaaa ctctcgattt ccgcttgcgt
       61 cagggaaaca atatcgcgct ctggattcgt tgggacgaac tcagcaacac tttcagcctg
      121 tgcgatcgca gtatcaagaa gtcaaagggc cgcaggggcg gcggtctacc aggctccccc
      181 ataacttgcg gggctggcga gctgcccggc agccctctcg tcttggaggg tgctttgggt
      241 cagcttcatt tagcggaaac cacagtgatt gggagcggcc cgaccggcca gagtgtaact
      301 ctcaatttgg ccatcgagct cggcccgaac gcaaccggac attacgccgt cgagttagcg
      361 gcaactgatg attttggcgc cgaggatgat ttcgtccagg ctagcgatgt tctaatccgg
      421 cccgctaaaa gacgttagct gttgtagaca atggcgtcac cccgggtgag tcgaaaccga
      481 cagggttcaa atcggaaaag taatcggggg cagtcttcta tttatttgtg atcgtgactt
      541 gctctcaggt gatcgcattg gtcacgcctg gtgaaacgaa tagttcaccg cgtccagttc
      601 catgattcgc ggctggcatc gattaaattt ttaacgctgc gcagcgcgcg cacgcgggcg
      661 accgcgcgtg cgtcgccggc ccagacgctc gagctggaac gcacttgctc gagcgccagc
      721 tctttcatcg gccttgctat cctggtcaat agcatcaagt ccgtgctagg acgaattgct
      781 gcgcccaagg tgccggctgt ctaattatcc gcaaaagaat ggccagagtt atcgacgatc
      841 gacgaggtca aaagcttcgt tcgcttccga gacaaatttt tggacctttt cacggctctt
      901 gcgacccgtc gagtcttcga cgccggtgtg ggagtcgact ccggctggtc caacttccag
      961 aatcgcgctc ttgacgttct ccggcgtgag cccgccggcg agaatgatcg gtcgatcggc
     1021 aagttccacc aggcgcctac tcacgcgcca gtcgtgagtc ttgccggtgg cgcccgaggc
     1081 gccggtttct ggatcgaagg tgtcggtaat gaaaccatcg acaaacggcg acagctcccg
     1141 cagcgtggct tcgagagctt tttgctcgcg cattccgatc acaagactct tgagcacggt
     1201 taagttcgga tcgagagtct tcagacgctt cagctcatcg cgttcgatgt cgccatggag
     1261 ctgcacgatc cgggcgccga gcgcatgaca gaaagcggca atctcggtcg cctggtttag
     1321 ataagtgatt gccactccga aaactggcgg cgtcaggacc ttgataatcg ccgccgcctc
     1381 ctgctcggtg agatcctcac ggtgaaccgg gagccgaagc ggaaatccca gatagcgaac
     1441 accgcactgc tgaagcattt gcgcttcacc ggcgtcgatg atcccggcaa tctggattaa
     1501 attttggtcg agcatatttg gcgaacaatc ttagcaacct ctgtatctcg gtcttcgcgc
     1561 tgtccaacgc cggccaattc agcggccccg tttaccattg gcagatttgg aaagagagcc
     1621 acctggaacc tcgcactttg ccggttgtga ctccgacttt cttctcccta agtcctcaat
     1681 gagttgctca atgaagccca cctgaccaga caaaatcttt tccctggctt ctgagatgtt
     1741 gaaccaagcc gcgcgatcga cttccgggta ctgctgcata ttgccggact tgggcggcca
     1801 ctccagtgag aacgaattgc tgcgaatctg cgccgggtcg cagtcggctt caatcgccca
     1861 ggcatatact attttaccac caggttgctt cagcggatcg agacaacgaa actctccgga
     1921 aatcgtaaaa ccggtttctt ccgtaaactc acgctgggca gcctgcagca gctcctcgcc
     1981 agccgcgaat tcgccctttg gtatcgacca tgccccggca tctttctttc gccaaaacgg
     2041 tccaccggga tggaccagaa acacctcaac ctccggcgta cggcgatata acaaaagtcc
     2101 agcactagtt tttcgtgcca tgattcaact caattgatta cgtaacggcg tccgtatgac
     2161 aattggccaa ggcataggtt catatacggg gccgcgactg catcgctccg ctccgtttaa
     2221 atcgttctaa atgtcgcaag attgcgagac gcgcgaaacc gctgagtccg acagaaggct
     2281 tgacgagtta aagaaattga gtcgaagcaa gaaaccctag cggttgtgaa ctcagcgcct
     2341 cgtgccgact tgcgcgacgg ttgtcagttc aactcatcaa tccgtcgttg taaaaactct
     2401 tcggcacaaa attgaagaaa tgacctgcac cctcgatgat gcgttgcgag tgcggcgtgc
     2461 cggcagggat gttcactccg acggggctct cgacgacgac tttgtcatcg ccgatctgaa
     2521 actcgatttt gaggccggtc aattccgctc ccgtgccgat ggcaatgtgc tgcgtgtcgc
     2581 agttgtgccg gtgcatatcg acgtaatccg cctgggtcgg ttgcacgccc gcaacgctgc
     2641 gcacgacggt ataaaagtta gcctcgggct tcaccttgcc gtcgacaaaa acatagcgcg
     2701 tcccgggcgc cgcatcggtg tggtacttga tctcggttgt cggccgcact tcgggcggaa
     2761 aaatgtaacc ggcatatttg gagaattcgc gttcgccctt cgcaacatcg aacggcttat
     2821 cctcatgagt gtagccgcgg ttgcgaaata agacggtcac cgtgccggcg ccgcgcgtca
     2881 ctttgtactc gtgcactgcc ccggtgggaa tgtatacgca ggcgggcgcg acgatcttgt
     2941 gcacttttcc atcgaagata atttgcccct cgagccccgt cagatccggg ttgttaccga
     3001 taaaatagta gaactcgtca gtattgtggc agtgcggcac ctgataatcg gcaacctgcg
     3061 caggcaagtc tttagcttcg tggatcgcga cgtaaatatc ggcatcggga tgcgaatcgc
     3121 ggttgataaa tacgtaacgc tttaccggca gcgcgctcaa ttgacgcagc ggccgcttct
     3181 cttcatgctt gccgccgagc a
//
LOCUS       DATLCH010000329         8983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7265105, whole genome shotgun sequence.
ACCESSION   DATLCH010000329 DATLCH010000000
VERSION     DATLCH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7265105"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..510)
                     /locus_tag="VMZ02_09715"
     CDS             complement(169..510)
                     /locus_tag="VMZ02_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HUR72254.1"
                     /translation="MKTTLKSIAGLVLVANIAAGCAGGAITTREKGLGIGALGGATAG
                     GLIGTAVRHPLAGAMIGGVLGAGAGGLIGDQLQGRDIQGNAQDQAIQRNEQEILRQRQ
                     ELEQLKQRREY"
     gene            complement(686..1429)
                     /locus_tag="VMZ02_09720"
     CDS             complement(686..1429)
                     /locus_tag="VMZ02_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HUR72255.1"
                     /translation="MKRAKLSPARNLKFYRRKSAALAFVGLLPFALTACSIMDSPTPT
                     SPPSRRPPQDYGQPRQSYSTRAVDPQDSERLRRVMVPLVQAMDKPCRIDQVKVGIIDQ
                     NEINAGNAGNCQFLVTMGLLRRASDDQLRGVLAHELAHQDLGHVAKAQVLGATLNVLT
                     AGLQQLSPLAGAVAPIAGQLAVRSYGRNEEFNADKHGVDILRRAGYSKEVMVDALSWI
                     RRVSGDKGGGFLSTHPGLDDRIATVQKLR"
     gene            complement(1455..2315)
                     /locus_tag="VMZ02_09725"
     CDS             complement(1455..2315)
                     /locus_tag="VMZ02_09725"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HUR72256.1"
                     /translation="MEIPKLNWFLASLCVFGLLAGSVSQAGEATIKPENLPVYTPKFY
                     PFEKGEKEVYRASWNGLISVATAEVTTTPTMVDGKKVYQVRIDAKTGKALDLIWKMRD
                     TISSTFDAKSLSPSHYLFNQRENSRVIDTEAKLDATTKSWVVTRQQTGKKTRTYNFES
                     QNTFDPVTAVYLARSVDFKVGDKINFKIFGGRYHYLLELFVEKKEPVELPSGKTVEAY
                     KIVPHVQNLKKTGYASRLREAAIWISADERRLPVKLSSKILFGSVQLDLVEDKQGIRA
                     TAAKGERPAS"
     gene            2495..3649
                     /locus_tag="VMZ02_09730"
     CDS             2495..3649
                     /locus_tag="VMZ02_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72257.1"
                     /translation="MKMFRPVGLSIFLGLFSAALVDAQEIRQSTVANQSPLPSLNAIK
                     ACNEGPNSPECLDKLFGEALKKHTTLEALQLIQRYEKEDDDLRRDCHPVVHAIGRETY
                     RIKGTIHDSFSACDQTCHSGCYHGSVERFLRGEDIYAQANKHPSAAELKQKAALACDP
                     KLADRFRFQCFHGLGHALLFFTRYQLAPALDACDALPNGWSQSSCYGGVFMENVSNAT
                     PELRDVSATDYHYPCNKLAAKYRGECYVMQTSRMSEMGLTTERIFQECDKSGEYRYQC
                     AVSAGRDLSNYVRLGQVQPTAQKCQVAIGDSKRACIHGVVYALVDNTWDGRYAMPFCA
                     ALAEGSDQADCYRESITYLKDTFEKSAAEIAKECREHATPSERCVDLVAR"
     gene            3828..4217
                     /locus_tag="VMZ02_09735"
     CDS             3828..4217
                     /locus_tag="VMZ02_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR72258.1"
                     /translation="MELPLLMLIDDEFGVRESLKMVFSKEYRVLDSDSLAAALPKIEE
                     SRPSVVLLDVLMPRTDGLAALQQIKQIHPQCEIIMLTGINSRQLAAKAIELGAFDFIG
                     KPFDIVDLREKVGRALEQVAQRWAARL"
     gene            4487..5125
                     /locus_tag="VMZ02_09740"
     CDS             4487..5125
                     /locus_tag="VMZ02_09740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HUR72259.1"
                     /translation="MNSRSTNHRIKTVYLLILLCTLFASCSRLKQWAYQGGDRDQWQQ
                     PDKVIEALQIRQGAEIADLGAGGGYFTFHLAKATGSAGKVYAVDVDRGTNELIAEKAQ
                     KDGVDNVVTILAKANDPLLPKSGVDLIFTSNTYHHLDNRIIYFSNLRQYLKPGGRVAI
                     IDFDRRAWLEGLLRHYTPSEFIKREMEQAGYVLQLEPLFLDRQSFLVFTAKP"
     gene            complement(5253..5930)
                     /locus_tag="VMZ02_09745"
     CDS             complement(5253..5930)
                     /locus_tag="VMZ02_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72260.1"
                     /translation="MKTTAIISVTLLVSCLGASVGFSDSGSKGSSGPSSGSKGKSGNL
                     QLKLEAQMEPLIVAPATSPIAPDAEGEAKYRKQIQKGTIKTERFEARVKIPFPSPALG
                     IIDQASAQNADIQILLSSATTPTVAYASCALDLSEIETELEDGVTKTYAEYKVDVRRE
                     LRNSSYRQRQIHGICDVAPATVSIEAGTPAPVENDIVTAVLVIAPVPPAIVPTLTPIV
                     AGTVELD"
     gene            complement(6233..8497)
                     /locus_tag="VMZ02_09750"
     CDS             complement(6233..8497)
                     /locus_tag="VMZ02_09750"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HUR72261.1"
                     /translation="MKADNQPAQRIVLVSNRLPFTVAQQGDDIEFQQSAGGVASGLHA
                     LLTSNSCAIRENTEYLWIGWPGDAVPEVLRHRVRAEALSRYHALPVFLSADEIDAFYA
                     GFCNKTLWPLFHYFPQYTRHEPEYWTHYVKVNQAFAEAALEVLRPGDLLWIHDYHLML
                     LPRLIRARLPQLSIGFFLHIPFPHFEIFRLLPAQWRRDLLEGLLGADVIGFHTFDYAQ
                     YFLRCVLRVLGHESQMGKLIVDDRSVKVDAYPMGIPFDQFATAARSAPVREGMQAIRS
                     SLPHTKLVLSIDRQDYSKGILNRLEGFEAMLEACSEWRGQVTLVMVVVPSRIGIEDYE
                     QMKNQIEQLVGRINGKFGGVEWTPIVYQYRSLSFESLVSLYSICDVALVTPLRDGMNL
                     IAKEYVACRSEKNGVLILSEMAGAARELGEAIAVNPNSREEIASALIEALTMPAAEQR
                     RRMEIMQARLRRFDLGYWAKGFLAELAGVQPDRARVLGKLLAPAESRDLVEHFRAAKR
                     RLVILDFDGTLVPLIADPRDTKPSGALLSLLAGLTGDTKNEVVLLSGRDRMSLHNWFA
                     WMRIGLAAEHGVWLKKRAQEWRLAGHRSTEWKAKLKPLLDMYAERLPGALVEEKEYSL
                     VWHYRRADVEQGAMVARSLTDDLLNFTGNIDVQVVQDNKSVEVRSAGVGKAAALRYWL
                     RDEEFDFVLAIGDDHDDEELFAALPAHSFTIRVGIVRTAARFNLREPNDVVKLLEELR
                     WAQSSSKAAIHQSL"
BASE COUNT         2008 a         2585 c         2399 g         1991 t
ORIGIN      
        1 gccggcggcg agcggcaccg caccagccaa agccggtgcg gtgaggatca aaacgagccg
       61 caagctgctc cacatggcgt tcaattcgtc accgcctgta atcagcttcc aaaaactgaa
      121 cggggggcca aggccccccg tttggaaaaa ttttattaaa ttatccgact aatattcgcg
      181 ccgctgtttg agctgttcga gctcctggcg ctggcgtaag atttcttgtt cgttgcgctg
      241 gatcgcctga tcttgagcgt tgccctggat atctcgaccc tgcaactggt cgccgatcag
      301 cccaccggcg ccggcgccta gcacgccgcc gatcatcgcg ccagccaagg ggtgacgtac
      361 tgccgtaccg atgagtccgc cggccgtcgc accgcccaaa gcgccaatgc ccagcccttt
      421 ttcacgggtt gtaatcgccc cgcccgcgca accggccgct atgttcgcaa ctagcaccaa
      481 accggcaatt gatttcaaag tggtcttcat ggtctccgcc tccttggaaa attagacgga
      541 tgggtcgagg aaatgtttac tggctggcat ggagtcgaag cagaatattt ttggcggtag
      601 gccggattca aacgcgccct tgctgctcgc cggctttgcc ggaaatgacg gagccgggta
      661 caaggaaact atcgccgtag gaagcctagc gcagcttttg cactgtcgcg atccggtcgt
      721 cgagccccgg atgagtagat aggaaaccgc cccccttatc gccggagacc cggcgaatcc
      781 aactgagcgc gtcgaccatg acttccttgg aatagccggc acggcgcaga atatcgacgc
      841 catgcttgtc ggcattgaat tcttcgttgc ggccatagct gcgcaccgca agctgaccgg
      901 caatcggcgc tacggcaccg gcaagaggcg acagttgttg caagccggcg gttaaaacgt
      961 tgagggttgc gccgagcact tgtgccttgg cgacgtgtcc gagatcctga tgcgccaact
     1021 cgtgcgccag gacgccgcgt aactggtcgt ccgaagcgcg ccgcaacagt cccatggtca
     1081 ccaggaattg acagttcccg gcattaccgg cgttgatctc gttctgatcg ataatcccaa
     1141 ccttgacttg atcgattcgg caaggcttgt ccatggcctg caccaagggc accataacgc
     1201 ggcgtaaacg ctcggaatcc tgcgggtcga cggcacgcgt cgagtagctt tgtcgcggtt
     1261 ggccgtagtc ttgcggcggg cgccggctcg gcggcgaagt tggcgtcggc gaatccatta
     1321 ttgaacacgc cgtcaaggcg aacggtagca aaccaacgaa cgccagcgcc gcactcttac
     1381 ggcgatagaa ttttaggtta cgtgcagggg acaacttagc tcttttcatg gcaacaccat
     1441 tggtcaaaat tagtttaaga cgcggggcgt tcccctttgg ccgctgtagc ccggataccc
     1501 tgtttgtctt ccaccaggtc aagctgcacg ctgccaaaca gaatcttgct gctcagcttg
     1561 accggtaagc gtcgttcatc agcggaaatc cagattgccg cctcgcgcag tcgactggca
     1621 tagccagtct ttttcagatt ctgcacgtgc ggcacaatct tataggcttc gacagttttg
     1681 ccggacggca actccacagg ttcttttttc tcgacaaaca attcgagaag ataatgatag
     1741 cggccgccaa agattttgaa attgatctta tcaccgacct tgaaatcgac gctgcgagcc
     1801 aaatacaccg ccgtgactgg atcaaaggtg ttttgcgatt cgaaattata ggtcctggtt
     1861 ttcttgccgg tttgctggcg cgtcactacc caacttttgg tggtcgcatc gagtttggct
     1921 tcggtatcga taacccgcga gttctcgcgc tggttaaaga gataatggct cggtgatagg
     1981 gacttggcat caaacgtaga actgatcgtg tcgcgcatct tccatatcaa gtcgagagcc
     2041 ttgccggtct tcgcgtcgat tcgaacttga taaaccttct tgccatccac catggtcggg
     2101 gtcgtcgtta cctccgccgt ggcaaccgat atgagaccat tccacgaagc gcgatagact
     2161 tctttctcgc ccttctcgaa cggataaaat tttggagtgt aaaccggcag gttttccggc
     2221 ttgatggtcg cctctccggc ctgcgatacc gacccggcaa gcaacccgaa gacacataga
     2281 ctcgccaaga accagttgag tttcggtatt tccatgtcca tcctttagac gcagcgcagt
     2341 taaaaaggtt tagccgcaaa aatatcataa attcaggctt taccctagcg atacggccca
     2401 acgcttgata ctccgccgag cttggttaag taaccgaaaa tattgcagct gactggcggt
     2461 tgggctacat tctggagaat tcagaggagc acctatgaaa atgtttcgac cggttgggct
     2521 gtcgatattt ttgggtctgt ttagcgccgc gttggtggac gctcaggaaa tccgtcaatc
     2581 gacggtggca aaccagtctc cactgccatc gctgaatgcc atcaaggcct gcaatgaggg
     2641 tcccaacagc ccggagtgtc tcgacaaact atttggcgag gcgctaaaaa aacataccac
     2701 tctcgaagcc ctgcagttga ttcagcgcta tgaaaaagaa gacgatgacc tgcggcgcga
     2761 ctgccatccc gtggtccatg cgatcggccg ggaaacttat cggatcaaag gaaccattca
     2821 cgactcattt agcgcctgcg atcagacctg tcattcgggg tgctatcatg gctccgtgga
     2881 gcgttttctg cgcggcgaag atatttacgc ccaggccaac aagcatccga gcgccgccga
     2941 gctcaagcaa aaggccgcgc tcgcctgcga tcccaagcta gccgaccgct ttcgttttca
     3001 atgttttcat ggattgggcc acgcgctctt gttcttcacg cgctatcaac tcgcccccgc
     3061 gctcgacgcc tgcgacgcgc tgcccaacgg atggagccaa tcatcgtgct acggcggagt
     3121 atttatggag aacgtatcta atgccacgcc tgaattgcgc gacgtgagcg cgaccgacta
     3181 tcactatccg tgcaacaagt tagccgccaa gtaccgcggc gaatgttatg tcatgcaaac
     3241 ctcgcgcatg agcgaaatgg gtctgacaac ggaaagaatc tttcaggaat gcgacaagag
     3301 cggcgaatat cgttatcaat gcgccgtctc ggcgggtcgc gatctgtcga actacgtacg
     3361 ccttggacag gtccagccga ccgcgcagaa gtgtcaggtg gccataggcg attccaaacg
     3421 cgcgtgcatt cacggcgtgg tctatgccct tgtcgacaac acttgggacg gccgctacgc
     3481 catgcccttt tgcgccgctt tggccgaagg cagcgatcag gctgactgtt accgtgagag
     3541 cattacgtat ctcaaagaca cttttgaaaa atccgccgcg gaaatcgcca aagaatgtcg
     3601 cgaacatgcg acgccgtccg agcgctgcgt cgacctcgtc gcccgctaag cgaatcctgt
     3661 accgttgcgc cggtttattg ctcggctgac ttagattttc gccgcgttgc gaaacgccgc
     3721 agagttcctc taagctttca cgtcagataa tcgaatgtcg cttgtgtgcg ccggtcgtaa
     3781 gtgcaaaagt aagcatcatc acgctgggaa acatcgctcg cccgatcatg gaactaccac
     3841 tcttaatgct catcgacgac gagttcggcg tccgcgagtc gcttaaaatg gtattttcca
     3901 aagagtaccg cgttctcgac agcgactcgc tcgcggcagc gcttcctaag atcgaggagt
     3961 cccgcccaag tgtcgtcctg ctcgatgtgc tcatgccgag aaccgacgga ctcgccgccc
     4021 tgcaacagat caagcagatc catcctcagt gcgaaatcat catgctcacc ggcatcaact
     4081 cccgacagct cgccgccaag gcaatcgagc tcggcgcgtt tgacttcatc ggtaaaccct
     4141 tcgatatcgt cgatctgcgg gaaaaagtcg gtcgcgctct tgaacaagtt gcccaacgat
     4201 gggcagcacg tttataagaa caaaaatccc gcgcaaaaaa aagctcgact gcgtttcagc
     4261 acgaaattta ggaagccagc gtcggaatga tattaaaaat ccgcaacatt tgcgcaacaa
     4321 ggcactccca tcgcaggtca ttttaaccga ggggaggact ttttcctgat tgctcggccg
     4381 ctgacatgac cggcattcct gtcggttttc agattggcgc ttttgctgga gctattcgac
     4441 tttccatgcg gcgccgtcgc cgcacagtgc tctgcgattg agtactatga attccagatc
     4501 caccaaccat cgcatcaaga ctgtttacct gttgatcctc ttatgcactc tgtttgcaag
     4561 ctgcagcagg cttaaacaat gggcttacca aggtggcgat cgcgatcagt ggcagcagcc
     4621 cgacaaagtc atcgaggcgc tgcaaatccg ccaaggggct gagatcgccg atctcggcgc
     4681 aggcggaggc tatttcactt ttcatctggc caaggcaact ggctctgccg gtaaagtcta
     4741 tgctgtcgat gttgacaggg gcaccaacga attgatcgct gaaaaagcgc aaaaggacgg
     4801 cgtcgacaac gtcgtaacga tcttggcaaa agccaacgac ccgctgttgc ccaaatccgg
     4861 cgtcgacctg atctttacca gcaacaccta tcatcatctc gataaccgca ttatttattt
     4921 ttcaaacctg cgccaatact tgaaacccgg aggccgcgtc gcgattatcg acttcgatcg
     4981 gcgcgcctgg ctcgaaggcc tgttgcgcca ctacacgccg agcgaattca tcaagcggga
     5041 aatggaacag gcgggttacg tgctgcagct agaaccgctc ttcctcgacc gccaatcgtt
     5101 tctagttttt acggcgaagc cttgagtcga cttgccgcat ggagattagg tgagtcgccg
     5161 agagcgcggc acgattgatg gagcgtcggt gggcgtaaat aaccacggcg gctggcgcag
     5221 caatcacgcc agccgccatt gacggcgata aactaatcga gctcgaccgt gcccgccaca
     5281 atcggggtca aagtcggaac aatcgcgggc gggactggag cgattaccag aactgcagtt
     5341 acaatatcgt tttcgaccgg cgcgggcgtg ccggcttcga tggacacggt cgccggggca
     5401 acgtcgcaaa taccgtggat ctggcgctgc cggtacgacg aattgcgcag ctctctgcgt
     5461 acgtcgactt tatattcagc ataggttttg gtcacaccat cttcgagctc ggtttcgatt
     5521 tcactcagat ccagagcgca ggaggcgtaa gcgacggtcg gtgtcgtcgc cgagctcaag
     5581 agtatctgga tgtccgcgtt ctgcgccgac gcttgatcta taattcccag cgcaggactc
     5641 ggaaaaggga tcttaaccct cgcttcaaag cgttcagtct tgattgttcc cttttgaatt
     5701 tgtttgcgat atttcgcctc gccttcggca tccggagcga tcggcgacgt cgccggcgcg
     5761 acaatcagcg gttccatctg tgcctcgagc ttgagttgca agtttcccga cttgcccttc
     5821 gatccactgc tgggtccgct tgagcctttc gatccgctgt cagaaaaacc aacgctggca
     5881 cccaagcagc tgaccaagag cgtcacgctt atgattgcgg ttgttttcat ataaatcctc
     5941 gatccctcct tagttgtttt cgccgaatca aagcaaatcc agttccaccg tgagcgcaac
     6001 aatcgcattg tcagcttcga ggacgatttg ccaatcccgc gcgaaactcg ccgcgcccga
     6061 acgcgcagat aggttgaata tggtaactaa attgggcggt ttgagagcaa aaccaaagcg
     6121 cttctcagga acgcgtgaaa agcactggtt tatatgtcca atgaatcgat tgattcaagc
     6181 gctaccctgg gcatttagcc acattaacga agcgatcggg tgccgttttc gttcacaggc
     6241 tttgatgaat cgccgccttg gacgagcttt gcgcccagcg tagctcctcg agcagcttga
     6301 cgacgtcgtt gggctcgcgc aggttgaagc gagcagcggt tcgaacaata ccgacacgga
     6361 tggtaaagct gtgtgccggc agcgcggcaa acagctcctc atcgtcgtga tcgtccccta
     6421 tggcaaggac aaaatcgaat tcttcatcgc gcagccagta acgcaacgcc gccgccttgc
     6481 cgacgccggc gcttctaact tcaaccgatt tattgtcttg gaccacctga acgtcgatgt
     6541 tgccggtaaa gtttagcaga tcgtcggtca gtgagcgcgc caccatcgcg ccctgctcga
     6601 catcggcgcg acgataatgc cagaccaatg aatattcttt ctcctcgacc aaagcaccgg
     6661 gcagacgctc ggcgtacata tcgaggagcg gtttgagttt agctttccat tcggtgctcc
     6721 ggtgacccgc caagcgccat tcttgagcgc gctttttgag ccatacgccg tgttcggcgg
     6781 cgagaccgat gcgcatccaa gcaaaccagt tgtgcaaact catgcggtct cgcccactga
     6841 gcagtacgac ttcgtttttc gtatcaccgg ttaaccccgc cagcaggctg agcaatgcgc
     6901 cgctcggttt ggtatcgcgc ggatcggcga taagcggcac caacgtgccg tcgaagtcga
     6961 gtatcaccaa gcggcgtttc gccgcgcgaa agtgctcgac gagatcgcgg gactcggccg
     7021 gagcgagtag cttgccgagt acgcgcgcgc gatccggttg tacgccggcg agctcggcga
     7081 gaaacccctt ggcccaatag ccgagatcga agcggcgtaa acgcgcctgc ataatctcca
     7141 tacgccggcg ttgctccgca gccggcatgg tcagggcttc aatcaacgcg ctcgcaatct
     7201 cttcccgact gttgggattg acggcaatcg cttcgccaag ttcgcgcgcc gcaccggcca
     7261 tctcactgag gatcagcaca ccgttttttt cgctccggca ggcgacatac tccttggcga
     7321 tcaagttcat gccatcgcgc agcggcgtta ccagcgcgac gtcgcagatg gaataaagtg
     7381 aaacaagaga ctcgaaagat agactgcggt actggtaaac gatcggcgtc cattcgacgc
     7441 cgccaaactt accattgatt cggccgacca gctgttcaat ctggttcttc atttgctcgt
     7501 agtcttcgat accgatacgc gacggcacca cgaccatcac caaagtgacc tgaccgcgcc
     7561 attcgctgca ggcttcgagc atcgcttcga aaccttccag gcggttcaga atccccttgg
     7621 aatagtcttg ccggtcaatg gacaaaacca gctttgtgtg gggcaaactc gagcgaatcg
     7681 cctgcatccc ttcacgcacg ggagcactgc gcgccgcggt cgcgaattga tcgaagggaa
     7741 tacccatcgg gtaagcgtcc actttcacgc tgcggtcgtc gactatcaat ttgcccattt
     7801 ggctttcatg gccgagcaca cggagcacgc agcgcagaaa atattgggcg taatcgaacg
     7861 tatggaaacc aatgacgtcg gcgccgagca agccttccag caggtcgcgg cgccactgtg
     7921 cgggcagcag gcggaaaatc tcaaaatgag gaaacgggat gtgcaagaag aagccgatag
     7981 aaagctgcgg caggcgggcg cgaatcagcc gcggcaagag catcaggtga taatcatgaa
     8041 tccataacag atcgccgggc cggagcactt ccaatgctgc ttcggcgaag gcctgattaa
     8101 ctttgacgta gtgggtccaa tattccggtt catggcgggt gtactgcggg aaataatgaa
     8161 acagaggcca aagtgtcttg ttgcagaatc ccgcgtaaaa agcatcgatc tcgtctgcgg
     8221 acaagaacac aggcaaagcg tgatagcgcg acagcgcttc ggcgcgcact cgatggcgca
     8281 gaacctcagg caccgcatcg ccgggccagc caatccaaag atactccgtg ttttcgcgga
     8341 tcgcgcaact gttcgaagtc aaaagcgcat gcaggcccga tgcgacgccg ccggcgctct
     8401 gttgaaattc aatatcatcg ccctgttgcg ccactgtgaa cggcaagcga ttggatacca
     8461 ggacgatcct ttgcgccggc tgattatcgg ctttcataac ttgatttgac ccccgcagga
     8521 tcaccttaat tgcgccagcg aaagaatgct cgccagcacg cacacgggcg cagaggcaaa
     8581 ctggtatggc ctgcccccgc gcctcaaggg ccggttaatt tttttcaggt ctacgggttc
     8641 cctggcctaa aaggaaccct gaagactttt cgttcaaaaa gttattcgct cggcgcgatc
     8701 tccctcacct tgccatcttt atcgttgatt ttcatctgcg tccgttcttt tgcgctggcg
     8761 gcaattcctc ggttttactg atagcgtctc ttcgcctttg tggtgcgcac aaaaaattgt
     8821 ttttcagttg tccattttta ctacgacgtt aaactcattc gccaggacgg ccggcggact
     8881 caccggtatc gccaaaccat taccgccaat gtgttcatgg tctaagctcg ttaatattta
     8941 tttccgaaac cggcctcgat ttcaaatcat cttattggca gtc
//
LOCUS       DATLCH010000330         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7911736, whole genome shotgun sequence.
ACCESSION   DATLCH010000330 DATLCH010000000
VERSION     DATLCH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7911736"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..911)
                     /locus_tag="VMZ02_09755"
     CDS             complement(9..911)
                     /locus_tag="VMZ02_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HUR72262.1"
                     /translation="MFSKLLIPLDGSKTSEKVLPFAHILAHTLKLPVELLEVVDISTA
                     TAHVVADKARYLDGIIAEGERASRERLAEVSASFAGAHIECKVERGRPAEAIIARAEA
                     EPGTLVAMATHGRSGISRWMMGSVAEKVLRGTSVPLFLVRASKESTPARPANIKSIIV
                     PLDGSELAESVLPTVAELAKCLDAEVVLGRAYELAASTYYGKEDYLPKYDEILRALKE
                     EVESYLHQKSAGLKARGVSKVSWVALEGSGAEEIVRYANGHPDVLLAMCTHGRTGVTR
                     WTLGSVTEKVVRHSNKPVLVLHAA"
     gene            complement(1068..2678)
                     /locus_tag="VMZ02_09760"
     CDS             complement(1068..2678)
                     /locus_tag="VMZ02_09760"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HUR72263.1"
                     /translation="MTWSQNDSRIGEMESDPNVELAAVAADQDSLVQAMLDPQFYPKP
                     PDTVTHKETHISHLFLAGELVFKLKKPVRYTFLDYSSLEKRRFYLEEELRLNRRLAPS
                     VYLGITPITRDEFRWRLGGWDAPAEHVLVMRRLPEKRMLPFLLDAGQVTPEMMHELAE
                     LLARFHRSAEPVHAVDPGQYPALVRRQWCENIIELKPFVGQVIDRDDLQAVDRFALNF
                     IDSQGELLARRAEQGWLRDVHGDLHAEHVCFAPEGIQVFDCIEFEPKFRQCDLASEIA
                     FLAMDLEARGHSGLVEPFLARYGELIQDDEFRKLLPFWKCYRALVRAKVFALRGAESC
                     ATARRYMAYALQLTRQPRKPFLILVSGFTGSGKSTVAKALSERLGVATINSDIVRKAL
                     AGKTGRQTEGYGEGIYSASMTEKTYAKMASDAETLIASGRGVILDATFTERAHRQRMV
                     GLATKFHIPYLFIRCVAAEESIRERLSRRASQGRDVSDGRWEIYLKQKDSYEAPDEID
                     CADFLELETDAPVEKLADTCEQFVRARL"
BASE COUNT          622 a          812 c          714 g          604 t
ORIGIN      
        1 caatcgcttc aagccgcatg cagcaccaac actggcttgt tcgagtggcg cacgactttt
       61 tcagtaacac tgccgagcgt ccagcgggtc acgccagttc tgccatgcgt gcacatcgcc
      121 agcagcacat cgggatggcc attggcatag cgcacgatct cctcggcacc tgagccttcg
      181 agcgctaccc aggacacttt agatacgccg cgggctttca gccctgcgga tttttggtgc
      241 agataacttt ccacttcttc cttgagcgcg cgcaatattt cgtcgtactt gggtaaataa
      301 tcctccttgc catagtacgt cgatgcggcg agttcatagg cgcggccgag aacgacctcg
      361 gcatcgagac acttggcgag ttcagccaca gtaggcagca ccgattcggc taactccgaa
      421 ccatcgagcg gcacgatgat ggatttgata ttagccgggc gggcgggggt cgactctttg
      481 ctggcgcgca cgaggaacaa cgggacgcta gttccgcgca atactttctc cgccacactc
      541 cccatcatcc aacggctaat tcccgaccga ccgtgcgtcg ccatcgctac taaagtaccc
      601 ggttctgctt cggctcttgc aatgatggct tcagccgggc gccctctctc caccttgcac
      661 tcgatatgag ctccggcgaa actggcagag acttccgcca ggcgttcgcg actcgcccgt
      721 tcgccttcag cgatgatgcc atcgaggtag cgcgctttgt cggcaacgac atgcgccgta
      781 gcggtcgaga tgtcgacaac ctccaaaagc tcgaccggca gctttagggt gtgagccaga
      841 atgtgtgcaa acggtagaac tttttccgag gtttttgagc catccaacgg gatcaacaac
      901 tttgaaaaca tacgctctcc ttcatcggaa gaaggcgcaa ctaacatgcc agtgcagaac
      961 gcgaattaat cgacgtaaat tactgaattc ttattacttt tgagaataaa atcaggagct
     1021 attcgcagca gtgaaaaaac gtcggttatt cgcctaaaaa aggtgtttca aagccgtgcg
     1081 cggacgaatt gttcgcacgt gtccgctaac ttctcgaccg gcgcgtcagt ttcgagttcg
     1141 agaaaatcag cgcaatctat ctcgtccgga gcttcatagc tgtctttttg ctttaggtag
     1201 atttcccacc gaccgtcaga aacgtcccgc ccttgggacg cccttcgact caatcgctct
     1261 cgaatggatt cctcagcagc gacacagcga atgaagagat aaggaatatg aaattttgtc
     1321 gccaaaccta ccatgcgctg ccgatgcgcg cgctcggtaa atgtggcgtc caaaatcacc
     1381 cctcgcccgc tggcaattag cgtttcggca tcgctcgcca tttttgcgta ggtcttctcg
     1441 gtcatgctcg cgctataaat tccctcgccg tagccttcgg tctggcgccc ggttttaccc
     1501 gccaaagcct tacgaacgat atccgaattg attgtcgcga cgccgagtcg ctcgctcaac
     1561 gctttggcaa ccgtcgactt gccgctgccg gtaaagccgc tgacgagaat caagaaaggc
     1621 ttccgcggct gcctcgtcaa ttgtaaggca taagccatgt agcgtcgcgc ggtagcgcag
     1681 ctctcggcgc cgcgcaatgc aaatactttc gcgcgcacca acgcacggta acatttccaa
     1741 aatggcaaga gctttcggaa ctcgtcgtct tgaatcagtt cgccgtagcg cgcaagaaat
     1801 ggctcgacca aaccgctatg gccgcgcgcc tcgagatcca tcgccaaaaa agctatttcg
     1861 gaggcgagat cgcattggcg gaacttgggc tcaaactcga tgcaatcgaa aacctgaatt
     1921 ccttcgggag caaaacagac gtgctccgcg tggaggtcgc catgaacgtc acggagccaa
     1981 ccctgttcag cgcgccgggc caagagttct ccctgcgagt cgataaagtt cagcgcgaag
     2041 cgatcaacgg cctgcaagtc gtctcgatcg atcacctgtc ccacgaatgg tttaagttcg
     2101 atgatgtttt cacaccattg gcgccgtacc agtgccggat actgcccagg gtcaacagcg
     2161 tggaccggct ccgcgcttcg atggaagcgc gccagtaatt ctgccagctc atgcatcatc
     2221 tccggcgtca cttgcccggc gtcgagtaaa aacggcagca tgcgcttttc tggcagccgg
     2281 cgcatcacca gcacatgctc cgcgggcgcg tcccagccgc cgagccgcca acggaactca
     2341 tcgcgagtga taggcgtgat accgaggtag accgacggag ctagccgccg attgaggcgc
     2401 agctcttcct ctaggtaaaa ccgccgtttc tcaagactcg aataatcgag aaacgtatag
     2461 cgcaccggtt tcttcaactt aaaaaccagt tccccagcca agaacagatg cgatatatgg
     2521 gtttccttgt gagtcaccgt gtccggcggt ttaggataga actgcgggtc gagcatcgct
     2581 tgcaccagcg agtcttggtc ggccgcaact gcagctaatt ctacgttagg atcgctttcc
     2641 atctcgccga ttcggctgtc gttctgtgac cacgtcaaaa aacatggatc gccatattgc
     2701 cggacggtgt tgttagcgat tgtcgatcac accgaacaaa aggcaagaat ga
//
LOCUS       DATLCH010000331         4651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7276320, whole genome shotgun sequence.
ACCESSION   DATLCH010000331 DATLCH010000000
VERSION     DATLCH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7276320"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VMZ02_09765"
     CDS             complement(<1..536)
                     /locus_tag="VMZ02_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HUR72264.1"
                     /translation="MAFDLTHVLVGVALLLAAFVKGATGLGFPLIATPMVALLLDIRV
                     AVTILILPNLLMDVTQVFRDGFPYPILRRFKSMIGLTVVGVFLGTMVLVLLPLWSLNL
                     CLGIMVIVFVGSNLLKFEFTISPAVEKRLSPLIGLLSGFLNGMTNAAGPVLAIYFYSL
                     KLEKRNFVKSVATIFMIT"
     gene            590..1006
                     /locus_tag="VMZ02_09770"
     CDS             590..1006
                     /locus_tag="VMZ02_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUR72265.1"
                     /translation="MIYPAVTVAWELAFSGAQLRPAYHFAPLMIWGYLQYRLCGRYRI
                     VRGGGGPGLETPPERLVATGLYAYTRNPMYLGHIIFLVGLTLTLKSWFAALVTIGSAL
                     WFHTRVIGDEKKLMQTLGQPYVDYLLSVRRWIPGLL"
     gene            complement(1048..2646)
                     /locus_tag="VMZ02_09775"
     CDS             complement(1048..2646)
                     /locus_tag="VMZ02_09775"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR72266.1"
                     /translation="MAIRAQYYHDFREHLKALEERGKLVRIQREINKDTELMPLVRWQ
                     FRGLDERDRKAFIFEKIIDSKGKRYAMPVTVGTLAATTEIYAIGMMCEPEEIAERWTQ
                     AQLNPFDPIKVTSAPVHEVVWQGQDLLNGHGLDMIPVPISTPGFDNAPYLTSANWITK
                     DPETGIYNIGNYRSQIKSANRTGGLFTTQHMGQHWRKAKAKGQRLEACIAIGVVPSIA
                     YSATAKIPYDFDEYRLAGGLAGAPVEVVQAKTVDLLVPATAEIIIEGTIPTDVVEPEG
                     PFGEYPGYMGHRTESPFLEVTCITHRRDAIYTALMSQFPPSESSKIKHTGTEKIIYKL
                     LRHDSGNSAVLDVALHDEVSGSGQAYCVIKMRNATKAEAWRALHATAGFTGSYAKICV
                     AVDEDIDILDPAMINWAMCYNMRPDEDVVISKGKMAGLDPSTYPPGMEKEDRRSASTS
                     SLLINAIRPWPYTPVSLPKKEFMERSKQIWEELELPPLKPRMPWYGYSLGAWTKQDEE
                     EADLASRGDYFETGKKQTGQRKKS"
     gene            2829..>4651
                     /locus_tag="VMZ02_09780"
     CDS             2829..>4651
                     /locus_tag="VMZ02_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HUR72267.1"
                     /translation="MNIKQPDLLPNLVNSASTPDVKRGLATRYFWVLAVLLVALFYLL
                     DLWIPAHLAIPICYAAALVLAVALPGKREKIAVALACTVMLVTDIFFIYSASGLPIWA
                     ALANQGFALLMVWSVTTLGLRHRTVEQDRRDSERIANERLALLNTIYTSAPVGLCFVD
                     LGLRYLSMNAALAEMNGRTPEFFIGKTIRDVIPEMADTIEVHYRRVIESGKPLLDVEI
                     KGATAARPDEGRYWLASYFPVYNPAGELLGVNVAVRDITARKQAEADTLFLLDLAECI
                     RFAANAEELTWAVAVALGEHAKVDRCAFVEIDVEQNQFVVQRDYHPHIASLVGSYPLE
                     SFGSLLSDVGRAGQTTTISDIAGDGRTRAHVESYRQLGIRAFIAAPLLRDGALVSALT
                     MASAHPREWSEREVSLINMVAERTWLAVEKLRLDEALRASGEALREADRRKDEFLATL
                     AHELRNPLSLVRNVVTLQQNPNNPLPDPHWGQDIIDRQVKYLTRLTDDLFDVSRITRE
                     KLVLQKEPLELTEVIKAAVESSRPLIDQRQHELTVTMSQDSIYVDADRLRLTQVFMNL
                     LNNAAKYTPNPGHIWLNVERAGDTVVVRVKDTGVGIGAESLP"
BASE COUNT          978 a         1245 c         1292 g         1136 t
ORIGIN      
        1 ttggtgatca tgaagatcgt cgcgaccgat ttgacgaagt tgcgtttttc taatttgagg
       61 ctgtagaaat aaatcgccaa aaccggtccg gccgcattcg tcatgccgtt taaaaatccg
      121 ctcaatagcc ctataagcgg cgacaatctc ttctcgacgg cgggggaaat tgtgaattcg
      181 aacttgagta agttcgatcc aacgaagacg atcaccataa tacctaggca gagatttagt
      241 gaccacagcg gcaataacac caaaaccatg gtgccgagaa aaacgccgac aacggtcaag
      301 ccaatcattg atttaaaacg ccgcagaatt gggtagggaa agccgtcgcg aaatacctgg
      361 gtaacgtcca tcaataaatt aggcagtatg agaatagtta cggcgacgcg gatgtcgagt
      421 aacaatgcca ccatgggtgt cgcgatgagc ggaaaaccaa ggccggtggc gcctttgacg
      481 aaggcagcaa gcaacaaggc gactcccacg agaacgtgtg ttagatcgaa agccatatcg
      541 ttttggcaat tctatgcgat tctttagccg cacgcctgtg cgaactttcg tgatttaccc
      601 agcggtcacc gtagcttggg aactagcgtt cagcggcgct cagctgcggc cggcttatca
      661 cttcgcgcca ctaatgatct ggggttactt gcagtatcga ctatgcggcc gttatcggat
      721 tgtccgtggc ggcggtggtc ccggactcga gactccgcca gagcgcctgg tggcaaccgg
      781 gctttacgcc tatacgcgca atcccatgta tttgggccac atcatttttc tcgtcggtct
      841 caccttgact ttaaagtctt ggttcgctgc gttggtcacc attggcagcg cgctttggtt
      901 tcacacccgg gtgatcggcg acgaaaagaa gctgatgcaa acgctcggtc agccctacgt
      961 ggattatctg ttgtcggtgc gccgttggat accagggctc ctttaaggga atacaaactt
     1021 cgtccttccg ccttcatgct ttgttgctta cgactttttc cgctgcccag tctgcttttt
     1081 gccggtttcg aagtagtcgc ctctgcttgc taaatccgct tcttcctcgt cctgcttggt
     1141 ccatgctccc aaactgtagc cgtaccacgg catgcgcggt ttgagcggcg gcaactcgag
     1201 ctcctcccaa atctgtttag atcgctccat gaactctttt ttcggcagcg acaccggcgt
     1261 atagggccag ggacggatcg cattgatcag caacgacgaa gttgaggcgg agcgccggtc
     1321 ttccttttcc attcctgggg gataggtcga cggatccagt ccggccattt tccccttgga
     1381 aataaccaca tcttcatcag ggcgcatgtt gtagcacatt gcccaattga tcattgcggg
     1441 atcgagaatg tcgatatctt catccaccgc aacgcagatt ttggcgtagc tgccggtaaa
     1501 accggcggtg gcgtggaggg cgcgccaggc ttcggccttg gtcgcattgc gcatctttat
     1561 gacgcaatag gcctgaccgc tgccactgac ctcgtcatgc agcgcgacgt caagcaccgc
     1621 cgagttgccg ctgtcgtgac gcaggagttt ataaattatt ttctccgtgc cggtatgttt
     1681 aatcttgctc gactcgctgg gtggaaattg gctcattaaa gctgtgtaaa tcgcgtcgcg
     1741 ccgatgcgtg atgcaggtaa cttcaagaaa tggcgattcg gtgcgatggc ccatgtaacc
     1801 cgggtactcc ccaaatgggc cttccggctc gacgacgtca gtgggaatag tcccttcaat
     1861 gatgatttca gcggtggcgg gcacgagtaa atctacggtt tttgcttgta caacctccac
     1921 tggcgcgccg gccaatccgc cagcgagacg gtattcgtcg aaatcgtagg gtatttttgc
     1981 cgttgccgag taggcaatgc ttggcacgac gccgatggcg atgcaggctt caaggcgctg
     2041 gcctttggct ttcgccttgc gccagtgctg ccccatatgt tgcgtggtga acaggccacc
     2101 ggtgcggttc gccgacttga tctgactgcg gtagttgccg atgttataaa tgccggtttc
     2161 gggatctttg gtaatccagt tggcagaggt caaataaggc gcattatcga agcccggtgt
     2221 cgagattggc acgggaatca tgtcgagccc atggccatta agaaggtctt ggccttgcca
     2281 gacaacctca tggaccggtg ccgaagtcac tttgatgggg tcgaacgggt taagttgtgc
     2341 ttgagtccaa cgctcggcga tctcctcagg ctcacacatc atgccgatgg cgtagatttc
     2401 cgtggttgca gccaaagtcc ctacagtcac cggcatcgca taacgtttgc ccttcgaatc
     2461 gattattttc tcaaatatga atgctttgcg atcgcgttca tcgagcccgc gaaactgcca
     2521 acgcacgagc ggcatcagtt cggtgtcttt attgatttcc cgctggatgc ggaccagctt
     2581 gccgcgctct tccagcgcct ttaaatgttc acgaaagtca tggtaatact gcgcccggat
     2641 ggccatggat atctcctgtc gtcgaaaaac tctcgtcatc tattgtctgg gccatcgcgg
     2701 gttgtcaagc gttcgagcac gagtcatccc gatgaggcgg taatcgcgcg ggaaatcctt
     2761 gtttgctcct gagtaggtct gctacactat cgccttattc ctgagtattt gctttccgct
     2821 tagaagtcat gaatatcaaa cagccggatc tgcttcccaa tttggtcaac agcgcctcaa
     2881 ctccagacgt gaagcgcggt ttggcgacgc ggtacttttg ggtcttggcg gtgctgttag
     2941 tcgcactttt ctatcttctc gatttgtgga ttcccgccca tcttgcaatt ccgatctgct
     3001 acgccgccgc tttagtctta gcggtggcgt tgcctggaaa gcgcgagaag atcgccgtgg
     3061 ctctggcctg tacggtgatg ctcgtcacag atattttttt catctactcg gcatcgggtc
     3121 tgccaatatg ggctgcattg gcgaaccaag ggttcgccct gctgatggtt tggagcgtaa
     3181 cgacattggg cctgcgccac cgaactgtgg aacaagaccg gcgcgatagc gagcggattg
     3241 ccaatgaacg gctcgcgctg ctcaatacca tttatacctc ggcgcccgta gggttatgtt
     3301 ttgtcgatct cggtttgcgc tatttgtcga tgaatgcagc tctggccgag atgaacggcc
     3361 ggacgccgga gttttttatc ggcaagacga ttcgcgatgt cattccggag atggccgata
     3421 ccattgaagt tcattaccgc cgcgtcatcg aaagcgggaa gccgctcctg gatgtggaaa
     3481 tcaaaggcgc cactgcggcg cgacccgacg agggacgata ttggctggca agttactttc
     3541 cagtgtacaa cccggccggc gaattactcg gagttaacgt cgcggtgcgc gatatcacag
     3601 cgcgcaagca ggctgaggcg gatacgcttt ttctgctcga tcttgccgaa tgcattcgct
     3661 tcgcggccaa tgccgaagag ctcacgtggg ctgtggcggt ggctctgggg gaacatgcca
     3721 aagtcgaccg ctgcgcgttt gtcgagatcg atgttgaaca gaatcaattt gtcgttcagc
     3781 gcgattatca tccgcatatc gcctcgctgg tgggcagcta tccgctagaa tcgttcggaa
     3841 gcttgttaag cgacgtcggt cgcgccggtc aaaccacaac gatctcggac atagccggcg
     3901 atgggcgtac ccgcgcacat gtcgaatcgt accgccagct cggcattcgc gccttcattg
     3961 cggcgccgct attacgcgat ggtgctttag tttcggcctt gaccatggcg agtgcccatc
     4021 cgcgcgagtg gagcgagcgt gaagtttcgc tgatcaacat ggttgccgaa cggacttggc
     4081 tcgcggtgga aaagcttcgt ctcgatgaag cactccgggc cagtggcgag gcgttacgtg
     4141 aagccgatcg gcgcaaggat gagttcttgg ctaccctcgc ccacgagctg cgcaatccat
     4201 tgtctctagt tcgcaatgtc gtgacgctgc aacagaatcc gaataacccg ctgccggatc
     4261 cgcactgggg ccaggatatc attgatcgcc aagtcaaata tcttacgagg ctgaccgacg
     4321 atctctttga tgtcagccgc attacgcgag aaaagttagt cttgcaaaag gaacctctcg
     4381 agctgaccga agtaatcaaa gcggcggtgg aatcgagccg acccttgatc gaccaacggc
     4441 agcacgaatt gacggtaacc atgtcgcagg attcgattta cgtcgatgcc gatcggcttc
     4501 ggctcactca agttttcatg aatcttttga acaacgctgc caagtacacg ccgaatcccg
     4561 gacatatttg gctcaatgtc gagcgcgctg gcgataccgt ggttgtccgc gtgaaagaca
     4621 ctggagtcgg tatcggagcg gaaagcctgc c
//
LOCUS       DATLCH010000332         7176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6434097, whole genome shotgun sequence.
ACCESSION   DATLCH010000332 DATLCH010000000
VERSION     DATLCH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6434097"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VMZ02_09785"
     CDS             <1..330
                     /locus_tag="VMZ02_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HUR72268.1"
                     /translation="NAEKIRSLGVIPKTLARAGAVSEETALEMSHGIRQRTGADIGLS
                     VTGVAGPSGGSSEKPVGTVWVSICQDQFHQAKLLDLIVLTDRERIIQGTAQAALNWLR
                     QRLLQKY"
     gene            332..901
                     /gene="thpR"
                     /locus_tag="VMZ02_09790"
     CDS             332..901
                     /gene="thpR"
                     /locus_tag="VMZ02_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HUR72269.1"
                     /translation="MIRVFIAIEISPGTIDRIASAVADFKPKLPEVRWTAPANFHLTV
                     KFIGDIETQRADAIAEALDPIITLFPRCTINAKGLGVFPGLRQPRILWVGFIGDELTQ
                     LAERVNTALLPLGFAGETRGFAPHLTIGRWRQPDRRTANLREEIARWRDHDFGATLVD
                     RLILFQSVLKPTGAAHSQLKVFKLNDQAD"
     gene            916..1968
                     /gene="recA"
                     /locus_tag="VMZ02_09795"
     CDS             916..1968
                     /gene="recA"
                     /locus_tag="VMZ02_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HUR72270.1"
                     /translation="MDVNREKAIDLAVSQIEKQFGKGAIMKLGEGGIVKDVQTISTGS
                     LGLDIALGIGGVPCGRVIEIYGPESSGKTTLALQIVAEAQKSGGMGAYIDAEHALDLG
                     YAQKLGVKTDDLLVSQPDHGEQALEIAETLVRSGAIDVLVIDSVAALVPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTGTIARSHTVVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRMDIRRIGALKDGDNIIGGRTRVKVVKNKMAPPFKEAEFDILYGTGISRDGEIVD
                     IGSEMGVIEKSGAWYSHAGERIGQGREQAKQFLKDHPETAQNIMTKIMDKYGLKAHDA
                     EALVEPAAADKKSKSK"
     gene            2063..2485
                     /locus_tag="VMZ02_09800"
     CDS             2063..2485
                     /locus_tag="VMZ02_09800"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HUR72271.1"
                     /translation="MSFRPRSELEVKNYLRQKEFEPQVIAVVIAKLQSLGYLDDLSFA
                     QIWARSRLESRGYGPRRVEQELRLKGIGAKVIQEAVQQSLQQVAEQESASKVLTKRFG
                     GTYLEDAKVQRRAVALLQRRGFSSKVIFNLVKYRIDDE"
     gene            2492..5128
                     /gene="alaS"
                     /locus_tag="VMZ02_09805"
     CDS             2492..5128
                     /gene="alaS"
                     /locus_tag="VMZ02_09805"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HUR72272.1"
                     /translation="MITANDIRKSFLDYFAKQGHTAVRSSSLVPDKDPTLLFTNAGMV
                     QFKNVFLGQQRLPYVRAASAQKCLRISGKHNDLEVVGRDTYHHTLFEMLGNWSFGDYY
                     KPEAIEWAWELLTKEWGLHKDKLYATVYLNDDEAEQLWFKISGLPRERVARFGDKENF
                     WEMGETGPCGPCSEIHLDRGPAACDMSGVAGHECRVNGDCARYIELWNLVFIQFNRAE
                     DRSLSELPAKHVDTGMGMERITAVLQNVLSNYDIDIMRALTATTERLTGKKYGVDPIA
                     DISYRVIDDHSRAVSFLIADGVMPSNEGRGYVLRRLLRRAARHGRLIGLKEPFLHEVA
                     KTVTAVMGDAYPELKQEEARIREVIRIEEERFGETLDRGLILLDNATAQLKAEKKHQL
                     SGEVAFRLYDTYGFPLDLTEDILRADNIEVDQKSFTELMEAQRSRGREAREAVTMESK
                     IQLDGQVRFIGYDRLDGQSSVIGIFGNSGTKHEAVQGEEIEIVTGETPFYGESGGQVG
                     DRGIITTARGDVVEIVDTQHPTPQLTSHRGRVKKGRIQVGDQVALNVDVRHRRKIMLN
                     HSATHILHAMLRKELGQHVRQAGSLVTPERLRFDFSHTGVIADDKLAAIEAQVNQRVR
                     EDASVSIRELTYDDAVRQGALAFFGDKYGDRVRVVKIGDFSTELCGGTHIRASGEIGL
                     FKLNFEGGVAAGVRRIEAFTGEGALELINSYESRLKEIGALIRGNPDDAVDKLKKLLE
                     RQKELEKEIAKLQGELEKDRIPDLIAQKQVIGSANALIAPVNGVDGKQLREIADQLKF
                     KLGSGIVILASAIDGNVNLVASVSKELTKQFHAGNIVKELAAMVGGGGGGRPDFAQAG
                     GKEPDKIPAALRRAEEIIKQAS"
     gene            5222..5650
                     /locus_tag="VMZ02_09810"
     CDS             5222..5650
                     /locus_tag="VMZ02_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HUR72273.1"
                     /translation="MITIHTKTISESTSGFCDIIDITEKFTEQIAATRMRSGTATLFV
                     SGSTAALTTIEHEAGLVDDLKEFLERFIPSDRRYHHDDRWGDDNGFSHLRATLFGPSL
                     AIPILDGKPCLGTWQQIVLLDFDNRPRSREIIVQLIGEYE"
     gene            5647..6672
                     /locus_tag="VMZ02_09815"
     CDS             5647..6672
                     /locus_tag="VMZ02_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HUR72274.1"
                     /translation="MKDEKKQIAGHVEEIGVNQIEFHDPRLFRDLLGQHDQHVKILQN
                     TLQLKIRVRGSAMEIEGDPLQVELASQIIRQLYSLLEKGYPVYASDVDYAIRILSGDN
                     RAKLQDIFLDTIYISAHKRTITPKSIAQKAYIDAIRRYDIVFGIGPAGTGKTYLAMAM
                     AVAELMKNNYVRIILTRPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALHDMVDFDRAR
                     KMVERGTIEVAPLAFMRGRTLNDSFVILDEAQNTTPEQMKMFLTRLGYGSKAVITGDA
                     TQIDLPAGKASGLKEAARILRGVHGIRFVTFTEKDVARHHLVQEIIAAYERDDNQNNA
                     RDLVEQA"
     gene            complement(6712..>7176)
                     /locus_tag="VMZ02_09820"
     CDS             complement(6712..>7176)
                     /locus_tag="VMZ02_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HUR72275.1"
                     /translation="GGPLLDSAGRLIGVNTAIYSPSGTSAGIGFAIPVDTVNRIATEL
                     IRSGKVTRPGLGIQVADEQIAERLGVSGVLIIDVARGSAAAKAGFQPTRRDNTGRVRL
                     GDIITAIDGRKIDSPNDLFLALETHKVGDFVNVTVLRNDKTVQLKIALEAVQ"
BASE COUNT         1732 a         2001 c         2020 g         1423 t
ORIGIN      
        1 aatgcggaga aaatccgctc gctcggcgtc attccaaaaa cgttggctcg cgccggcgcg
       61 gtgagcgaag agacggcttt agaaatgtcc cacggcatcc gccagcgcac cggtgcggat
      121 atcggattga gtgttaccgg agttgccggc ccttccggcg ggagctccga gaagccagtc
      181 ggcaccgtct gggtgagtat ttgccaggac caattccatc aagcaaaact cctcgattta
      241 atagttctca ccgaccgtga acggatcatt caaggcaccg cccaagcggc attgaactgg
      301 ctgcgccaac ggcttttgca aaaatactag cgtgatccgc gtctttatcg ccatcgaaat
      361 ctcgccagga acgattgacc gcatcgcctc cgctgtggcc gactttaaac ctaagttgcc
      421 ggaggttcgc tggacggccc cggcgaattt tcatctgact gtaaaattta tcggtgatat
      481 cgaaacgcag cgggctgatg ccattgctga ggccttggat cccatcataa ccctctttcc
      541 gcgttgcacc ataaatgcta aaggtctagg ggtatttccg gggttgcgac agccgagaat
      601 tttatgggtc ggcttcatcg gagatgagct gacgcagttg gcggagcgcg taaacacagc
      661 cttgttacct ctcggatttg ccggtgaaac gagaggtttt gcaccgcatc tgaccatcgg
      721 ccgctggcgc caacccgacc gccgaacggc taatctcaga gaagaaatag cacgctggcg
      781 cgatcacgac tttggcgcaa cacttgtcga tcgtctgatc ttgtttcaaa gcgtgctcaa
      841 gcccacaggc gcagcgcatt cgcaactaaa ggttttcaag ttgaacgacc aggcagattg
      901 attaggaggc aggcaatgga tgtgaatcgt gaaaaggcaa tcgacctcgc agtaagtcag
      961 atcgagaaac agttcggcaa gggcgcaatc atgaagctcg gcgagggcgg cattgtcaaa
     1021 gacgtgcaga ccatttcgac cggttccctg ggactcgaca tcgcgctcgg catcggcggc
     1081 gtaccgtgcg gtcgggtaat cgagatctac gggcctgagt cctccggcaa aaccaccctg
     1141 gcgctacaaa tcgttgccga agcgcaaaag agcggcggca tgggcgcgta tatcgacgcc
     1201 gaacatgcgc tggatctggg ctacgcccag aaactcggcg tcaagaccga tgacttgctc
     1261 gtttcgcaac cggatcacgg cgagcaggcg ttggaaatcg ccgagacttt ggtgcgcagt
     1321 ggcgccattg atgtcctggt catcgactcg gtggcggcgc ttgtgcccaa agccgaaatc
     1381 gaaggcgaga tgggcgactc gcacatggga ttacaagcac gcctcatgtc gcaagctttg
     1441 cgcaaattga ccggcaccat cgctcgttcg cacactgtag tgattttcat taaccagatc
     1501 cgcatgaaga tcggcgtcat gtttggcaac ccggagacca ccaccggcgg caacgcgctc
     1561 aagttctacg cctcagtgcg catggatatc cggcgcattg gcgcgctgaa agatggcgat
     1621 aatattatcg gcggccgcac ccgtgtcaaa gtcgtcaaga acaaaatggc gccgccgttt
     1681 aaagaagctg aatttgacat tctttacggc accgggattt ctcgcgatgg cgagatcgtt
     1741 gatatcggca gtgagatggg cgtcatcgag aagagcggcg cgtggtattc ccatgccggc
     1801 gagcgcatcg gtcaaggccg ggaacaggcc aagcagtttc tcaaggatca tccggagaca
     1861 gcgcaaaaca ttatgaccaa gatcatggat aaatacggtt tgaaagcgca cgatgcggag
     1921 gccttggtcg aacctgccgc ggcggataaa aagagcaaaa gcaagtaact ttgaagaagt
     1981 cggatagacg ctgaaaagat cgaaaaccag cgctgcggcc gtcgactcgc ccgaacaggc
     2041 actgcaatca gctttacgct acttgagctt tcgaccgcgc agtgagttgg aagtcaaaaa
     2101 ttacctgcgc cagaaagagt tcgagccgca agttatcgcc gtcgtgatcg ccaagctcca
     2161 atcactcggc tatctcgacg atttatcctt cgcccagatc tgggcgcgtt cccgcctgga
     2221 gagccgcggc tatggtccga gacgggtcga gcaggagctt cgcctcaagg gtatcggtgc
     2281 caaagtgatt caggaagccg tgcaacagag tttgcagcag gtggccgaac aggaaagcgc
     2341 cagtaaagtt ttaaccaaaa ggttcggcgg cacatacctg gaagacgcca aagtgcagcg
     2401 ccgtgctgtg gcgctgctgc aacggcgcgg cttcagcagc aaagtgattt ttaacctggt
     2461 aaaatatcgt atcgacgatg agtagaaggc tatgattacc gctaacgaca tccgcaaatc
     2521 ttttttggac tatttcgcta aacaaggcca caccgccgtg cgcagttcat cgttggtacc
     2581 cgataaagat ccgacgctgc tattcaccaa cgccggcatg gtgcagttca agaacgtctt
     2641 cttagggcaa caacgcttgc cctatgtgcg cgccgccagc gcgcaaaaat gtttgcgcat
     2701 cagcggcaaa cataacgacc tggaagtcgt cggccgcgac acgtatcacc acacactatt
     2761 cgaaatgctc ggcaactggt cgttcggcga ctattacaaa ccggaggcca tcgagtgggc
     2821 ttgggagctt ctaaccaaag agtggggtct gcacaaggac aaactctacg ccacagttta
     2881 tttgaatgat gacgaggccg agcagctctg gttcaagatc agcggactac cgcgcgagcg
     2941 ggtggcgcgc ttcggtgata aagaaaactt ttgggaaatg ggcgaaaccg gcccgtgcgg
     3001 gccatgcagt gaaattcatc tcgaccgcgg cccggcggct tgcgacatgt ccggcgttgc
     3061 cggccatgaa tgtagagtca atggcgattg cgctcgctac atcgagctgt ggaatttggt
     3121 cttcatccaa ttcaaccgcg ccgaagatcg cagtctgtcg gaactgccgg ccaaacatgt
     3181 cgacaccggc atgggcatgg aacgcattac cgcggtgctg caaaacgtac tgtccaatta
     3241 cgacattgat ataatgcgcg cgcttaccgc caccacggag aggctcaccg gcaagaagta
     3301 tggcgtcgac ccaattgcgg atatctcata ccgggtcatc gacgatcact ctcgtgctgt
     3361 cagcttcttg atcgccgacg gcgtcatgcc gagcaatgaa ggacgggggt atgtgctgcg
     3421 ccgtctatta aggcgcgctg cgcgccatgg tcgcttaatt ggactgaaag aaccgttcct
     3481 ccatgaagtc gcaaaaaccg tcacggcggt catgggcgac gcctatccgg aactgaaaca
     3541 ggaagaggcg cgaatccgcg aggtcattcg tatcgaggag gaacgcttcg gcgagactct
     3601 cgaccgcggc ctgattttac tggacaacgc cacagcgcaa ttgaaagcgg aaaaaaaaca
     3661 ccagctgtcc ggcgaggtcg ctttccgctt atacgacacc tacggttttc cacttgatct
     3721 taccgaggat attctgcgcg ccgacaacat cgaagtcgac cagaaaagct tcaccgaact
     3781 catggaagcg caacgcagcc gcggccgcga agcgcgcgaa gcggtcacca tggaatcgaa
     3841 aatccaactc gatggccagg tccgtttcat tggctatgac cgtcttgacg gccaatcgtc
     3901 cgtcatcggt atattcggca attccggcac caagcatgaa gcggtgcaag gcgaggaaat
     3961 cgaaattgtc accggcgaga cgcccttcta tggcgaatcc ggcggtcaag tcggcgaccg
     4021 tggtatcatc acaaccgccc gcggcgacgt cgtcgaaatc gtcgataccc aacatccaac
     4081 gccgcagttg acctcccacc gcggcagagt gaagaaaggc cgcattcaag taggcgacca
     4141 ggtggcgctc aacgtcgatg tcagacatcg ccgcaaaata atgcttaacc attcggcgac
     4201 tcatattctc cacgcgatgc tgcgaaaaga attggggcag cacgtgcgcc aagccggctc
     4261 gctggtgact cctgagcggc tccgcttcga cttcagtcat accggcgtaa tcgcggacga
     4321 caaactggcc gcgatcgaag cgcaagtaaa ccaacgcgtc cgcgaagacg ccagcgtatc
     4381 aatccgggaa ctgacctatg acgatgccgt tcgccaaggc gccctggcgt tcttcggcga
     4441 caaatatggc gaccgtgtac gcgtggtcaa gattggtgat ttttccaccg agctatgcgg
     4501 tggcacgcac atccgtgcat ccggagagat cggtttgttc aaactcaact tcgaaggcgg
     4561 cgtcgccgcc ggcgtacggc gcatcgaagc ctttaccggc gagggtgcgc tggaattgat
     4621 caacagctac gagtcacgcc ttaaggagat cggtgcgctc attcgcggca accccgacga
     4681 tgcagtggat aagctaaaaa agctgcttga gcgccagaag gaattggaaa aagagatcgc
     4741 caaactgcag ggcgaattgg aaaaagatcg catccctgat ttgatcgcgc aaaagcaggt
     4801 cattggcagt gccaatgctt taatcgctcc ggtcaacgga gttgacggga aacaactccg
     4861 ggagatcgcg gatcagctta aatttaaact gggttccggg atagtgattc tagccagcgc
     4921 cattgacggt aacgtcaatc tagtcgcctc ggtcagcaaa gagctgacca aacaatttca
     4981 cgccggcaat attgtcaaag agctggccgc gatggtcggc ggcggcggcg gcggccgacc
     5041 ggacttcgct caggccggcg gtaaggagcc ggacaagatc ccggcagcgc tcagacgcgc
     5101 cgaggagatc ataaagcagg cgtcatgaag cgcgaatggt tggcaatctg gcgcttaccg
     5161 ccaagacatg cagtttgccg aattatgaac tcgtgccaag gagcgaggat cgaatcctag
     5221 cgtgatcaca attcatacca agacgatcag cgagtctacc agcggtttct gcgacatcat
     5281 cgacatcacc gaaaaattca cagaacaaat cgccgccacg cgcatgcgca gtggcactgc
     5341 cactcttttc gtcagcgggt ccacggcggc gctaacgacc attgaacatg aagcgggtct
     5401 agtcgacgat ctaaaggagt ttttagagag atttattccc tccgaccgcc gctatcacca
     5461 tgacgatcgt tggggtgacg acaacggctt ttcccatttg cgcgcaacgc tcttcgggcc
     5521 gtcgctggca atcccgatcc ttgacggcaa gccgtgtctc ggcacctggc aacaaattgt
     5581 cctgctggac ttcgataatc gcccacgcag ccgcgagatc atcgtccaac tcataggaga
     5641 atacgagtga aagacgaaaa aaagcagatc gccggtcacg tcgaggaaat tggcgtcaac
     5701 caaattgagt tccacgatcc acgcctgttc cgcgatttgt tgggccagca cgaccagcat
     5761 gtcaaaattc tccagaacac attgcaactt aaaatccgcg tgcgcggctc agcgatggag
     5821 atcgaaggcg acccgctgca agtcgaattg gcgagccaaa ttatacgcca gctctacagt
     5881 ttactcgaaa agggctaccc ggtttatgcc agcgatgtcg actacgcgat ccgcatcttg
     5941 agcggcgaca accgcgccaa gctgcaagac atctttctcg acacgatcta tatttcggcg
     6001 cacaagcgca caatcacgcc gaaaagtatc gcccaaaaag cttatatcga cgccattcgc
     6061 cgttacgaca ttgtctttgg catcggcccg gcgggtaccg gtaagacgta tctcgccatg
     6121 gcgatggccg tggcggaatt gatgaagaac aattacgtga gaattattct cactcgcccg
     6181 gcagtcgagg ccggcgagaa attaggcttc ctgccgggtg acctggcaga aaaggtcaat
     6241 ccctacctgc ggccgctcta tgatgcgctc cacgacatgg tcgatttcga tcgtgcgcgc
     6301 aaaatggtcg agcgcgggac catcgaagtc gcgccgctgg cgtttatgcg cggccgaacg
     6361 ctcaacgact cctttgtgat actcgacgag gcgcagaaca ccacgccgga gcaaatgaag
     6421 atgtttttga cacgcttggg ctacggctcc aaagcggtga tcaccggcga tgcgactcag
     6481 atcgatttac cggccgggaa ggcatccgga ctcaaggaag ctgcgcgcat tctgcgcggc
     6541 gtccatggca tccgcttcgt gacgtttact gaaaaagacg tggctcggca ccacttggtg
     6601 caagagatca tcgccgccta tgagcgcgat gacaatcaga ataatgctcg cgatctagta
     6661 gaacaagcct gagtgcgccg ttgaacggcc aaatcgcagc gtagtcgctt cttactgaac
     6721 cgcttcgaga gcgattttta gctggacggt cttatcgttt cgcaataccg tgacattcac
     6781 gaagtcgccg actttgtgag tttcgagtgc aagaaacaag tcattgggcg agtcgatttt
     6841 tctgccatcg atggcggtga taatgtcgcc cagtcgaacg cgcccggtgt tgtcacggcg
     6901 cgttggttgg aaacccgctt tcgccgcggc gctgccccgc gcgacatcaa ttatcaatac
     6961 tccgctcacg cccaagcgct cggcgatctg ttcatcggcg acttgaattc ccaagcctgg
     7021 ccgtgtgacc ttgccggagc gaatcagctc ggtggcgatg cggttcaccg tatcgacagg
     7081 gattgcgaaa ccgatcccag ccgacgtacc cgacggactg taaatcgccg tgttgacacc
     7141 aatcaagcgg ccggcgctgt cgagcaaggg gccgcc
//
LOCUS       DATLCH010000333         3097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3583420, whole genome shotgun sequence.
ACCESSION   DATLCH010000333 DATLCH010000000
VERSION     DATLCH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3583420"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VMZ02_09825"
     CDS             complement(<1..685)
                     /locus_tag="VMZ02_09825"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HUR72276.1"
                     /translation="MSGHSKWSTIKHKKAAKDAKRGKIFTKLIKEITVAARMGGGDIN
                     ANPRLRTAVLTARSNSMPSENIDRAIKKGTGELEGVTYEEIQYEGYGPGGVAIIAQVL
                     TDNKNRTVSEIRRMFSKHGGNMGETGCVGWMFDKKGILTVDKSQIDEDRLMDIALDAG
                     AEDVRDEGEVFEVIAPPEDFERVKERLDSEKIAIASAQVSLVPKNTVDVDAKNVEQVL
                     KLSEELEDHD"
     gene            847..1506
                     /locus_tag="VMZ02_09830"
     CDS             847..1506
                     /locus_tag="VMZ02_09830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HUR72277.1"
                     /translation="MNPTRRGLLLFALALVSACSVPPGRVRVPSAPITDARVSQTGIA
                     SWYGPGFHGKATASGDIYNQNDMTAAHQTLPLGSKVMVTNMENGRTAEVYVNDRGPFA
                     KGRIVDLSFAAAQSIDMVGPGTALVRLDVLDSPVPLRSIRPALDYTLQVGSFAQLENA
                     QQLRDRIVKNFDNVVITPLQSKDTTYYRVHLGNFSNRGAAEEMAKQVSQAGYSVIIME
                     K"
     gene            complement(1928..2845)
                     /locus_tag="VMZ02_09835"
     CDS             complement(1928..2845)
                     /locus_tag="VMZ02_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HUR72278.1"
                     /translation="MTTRRQALAALAKSSLAFFLAQSFLRSGASAAEGNPMAAPDFIG
                     KFTADNPGLMTLQGTNQYIVGRESGLVIDVALSADSNMDGIIEQAEAMGVKKITKILL
                     THIHSDHTGGALALRKRCGAKLGIHRSRKGYLGGEDFQYDDNDRIDFGGGELHVLHTP
                     GHESGHCCFYENVDKVLFSGDNILGYGTAVIHPPDGNMSDYMQTLERLLGFEMSLILP
                     GHGPLIGKPEAKVREYIKHRLEREQQIIAALRNNRNTIGEITEAIYVDASAALKRVAE
                     FSVQAHLEKLEREGKVKKEASRYLLLGAN"
BASE COUNT          656 a          903 c          812 g          726 t
ORIGIN      
        1 cgtcatgatc ttccagttct tcgctcagtt ttaagacttg ctcgacattt ttggcgtcga
       61 cgtcaacggt gttcttgggc accaggctaa cctgagcgct ggcgatggcg atcttttcgc
      121 tgtccagccg ttctttgact ctttcgaaat cctccggcgg ggcaatcact tcgaagacct
      181 cgccctcgtc gcggacatcc tcggcgccgg catcgagagc tatatccatc aaacggtcct
      241 cgtcgatctg cgacttgtcg acggtgagga tgcccttttt atcgaacatc cagccgacgc
      301 agccggtctc acccatgttg ccgccgtgct tggaaaacat ccggcgaatc tccgagacgg
      361 tgcgattttt attgtcggtc agcacctgcg cgatgatcgc cacaccgccc ggaccgtaac
      421 cttcgtattg aatctcttcg taggttacgc cttccaactc ccccgtgcct tttttaatcg
      481 ctcgatcgat attctcgctt ggcatgctgt tgctgcgggc ggttagaacc gcggtgcgca
      541 gtcgcggatt agcattgata tcgccgccgc ccatgcgcgc cgcgacagtg atttccttaa
      601 tcaacttagt gaagatctta ccgcgcttgg catccttggc ggctttttta tgcttgatcg
      661 tgctccactt agaatggcca gacatacttc aatcactccc tagtaagttg ccgccaagct
      721 aacacaaacc ctagagcggg taaactagca aaagagaact ccaagcggcg cggagaacct
      781 gtcaaattaa ctctcgcata acttttttcg tgcggtcttt gctgtataat cgccagcgaa
      841 gaaactatga acccgacccg acgcggttta ctgctgtttg ccttagctct cgtcagcgca
      901 tgttcggtgc cgccgggcag agttcgcgtg ccatcggcgc cgattactga cgcgcgcgtc
      961 agccagaccg gtattgcttc atggtacggc ccagggtttc acggtaaggc caccgccagc
     1021 ggcgacattt acaaccaaaa tgatatgacc gcggcgcatc agaccctgcc actcggaagc
     1081 aaagtgatgg tcaccaacat ggaaaacggc cgcaccgccg aagtatacgt caacgatcgc
     1141 ggtccgttcg ccaaaggccg aattgtcgac ctgtcttttg ccgcggccca gtcgatcgac
     1201 atggtgggac ccggcacggc gcttgtgcgg ctcgacgtgc tcgatagccc cgtgccgctg
     1261 cgctcgatcc gtccggcact cgattacact ctgcaagtcg gatcttttgc acaactggaa
     1321 aacgcccagc aattgcgcga ccgcatcgtc aagaacttcg acaatgtcgt gatcactccg
     1381 ctgcaatcaa aagatacaac ctactaccgt gttcacctcg gcaacttctc caaccggggc
     1441 gccgccgaag agatggccaa acaggtcagc caagccggtt actctgtgat catcatggag
     1501 aaatagatga gtgacgcccg tcctctcgta tgcgcgatca ggtcggcacg gctccatagt
     1561 caagcagttg aaccgttctc tctcgccgtg agaccgtttt tcggccgatt caaaaaaccg
     1621 tcccaacaag gcttcagcga acttaaatcg agcgtgcgag tcttcgaggt ctcgccctca
     1681 gcgtaatatc gacgacgaag cgctcgtggt ttctacgtgc cgttcaagcc gtcgcgaaaa
     1741 cgccaacgct tacgctgaca aaatcgcaaa gaagacgaaa cagcaatggg atcagtttcg
     1801 cgcgcgtgcg caaagacaat gcagtgttcg ctgagagagc ccgctcgccc actacgaggc
     1861 ggcgtggagc aatttgcaag ttgcggtaga actggagatt ccgggtttag atttaccgcc
     1921 aacccgctca attagcgccg agcaagaggt aacggctggc ttcttttttg accttgccct
     1981 cgcgttcgag cttttccaga tgcgcttgga cggaaaactc ggcgacccgc ttgagcgcgg
     2041 ccgacgcatc gacgtagatc gcttcagtaa tctcgccgat ggtattgcgg ttgttgcgca
     2101 gcgccgcgat tatctgctgt tcgcgttcga gccggtgctt aatgtactcg cgaaccttag
     2161 cttccggttt gccgatcagc gggccatggc ccggcaagat caaactcatt tcgaacccga
     2221 gcagacgctc gagcgtctgc atgtaatcgc tcatattgcc gtccggcggg tggatcaccg
     2281 cagtgccgta accgagaatg ttatcgccgc tgaacaacac cttatcgacg ttttcataga
     2341 agcagcaatg acccgactca tggccgggtg tgtgcagcac gtgcaattcg ccgccgccga
     2401 aatcgatgcg atcgttgtcg tcgtattgaa agtcctcacc gccgagatag cccttgcgcg
     2461 aacgatgaat gccgagtttc gcgccgcagc gtttgcgcag cgccagggct ccgccggtat
     2521 gatcgctgtg aatatgcgtt agtaagattt tggtgatttt tttcacgccc atggcctcgg
     2581 cctgctcgat gatgccgtcc atgttggaat cggcgctgag cgcgacatcg atgaccagtc
     2641 cggattctct accgacgata tattgattgg ttccctgcaa ggtcatgaga ccagggttgt
     2701 cggcggtgaa ttttccgata aaatccggcg ctgccatggg gtttccttcc gcggcgctcg
     2761 cgccgctgcg cagaaacgat tgagccaaaa aaaacgccag cgaacttttt gctaaagccg
     2821 ccagcgcttg tcttcgcgta gtcacgagtg aggtgtaatt cgcaatgagg ctttaccagg
     2881 gcacgagaag cacaagagtt ccaatattgt agatctaaac ttcctgtcct cgtgccttaa
     2941 tggtggtcgc caatttcaat tcgccagcaa ctgccgcaac acgtattcca aaatgccgcc
     3001 atgtttgtag tagtcgagct cagccggcgt atcgatgcgg cagatgactt taaattctgt
     3061 cgttttgcca tcgtccgccg tcgctttgac gattagc
//
LOCUS       DATLCH010000334         3835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8798468, whole genome shotgun sequence.
ACCESSION   DATLCH010000334 DATLCH010000000
VERSION     DATLCH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8798468"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VMZ02_09840"
     CDS             <1..833
                     /locus_tag="VMZ02_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HUR72279.1"
                     /translation="ERWMISSFTASSLIHLSLIPLAGLLIHAKPLKPVNMPVELIELP
                     RVEQPKKVEVVPPPPPLPPVPKPKLQNVTGPKLLSKPVLDASPLPPTGNTKEPVKETP
                     IAEPLAALPDNANAAKAGWNAGTPATQAEGSTAGAGNLFGKGDVGIVGGSGVAGGGGG
                     TGASGLGRGAAGDGTGGGAGSGEALSGMAKPLGGYQVKPRYPESAKRIGAQGVTTLRV
                     RVLETGRVSEVLVEQTAGFRDLDIAATEAVKKWLFEPARRGKEAVSVWVLLPVKFELH
                     "
     gene            842..1033
                     /locus_tag="VMZ02_09845"
     CDS             842..1033
                     /locus_tag="VMZ02_09845"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HUR72280.1"
                     /translation="MAETLMHCAAKSTAEPSILAPAAKKPRVESAHLFQGNNEIVIVH
                     QKEEYNLRITKNGKLILTK"
     gene            1115..3262
                     /locus_tag="VMZ02_09850"
     CDS             1115..3262
                     /locus_tag="VMZ02_09850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HUR72281.1"
                     /translation="MSRQQKRAIARQSEKTAFQKICLAAGEGMSKALAFSFALASHAV
                     AAEPKQDEPINLPPVVVEDQSSPYIVPQSSLSKFPEPLIDVPQSITIVPEKLLEEQAG
                     TTLRDALRNVPGITATAGEGGGAQGDVFTLRGFNARNDMYIDGVRDSGSYFRDSFNFD
                     SVEVLKGPSSTYFGRGSTGGVINQVSKTPRLDSSYNGDFTAGGNMYLRGTIDVNQPIP
                     QLLPNAAFRINLMAHRDDVVERDKIEVKRFGLAPSIAFGLGTPTQVTLSYLLQHEDNI
                     PDYGFPYLNGRPLRTDRSNFYGLSKDDNEDTTVNIGTLRVNHQFNDMFSLHNTLRYSN
                     VDRESAVTNPTAVPPNLNRMRPQRDTQESILSNQTDLTAKFSTYGLNHTVTTGLEVAR
                     ETFDLIRWASVGPNTTINNPNDSQPPSPKTLAAKSDTSALSFGIYAADQIKLNQYFDL
                     VGGVRWDYFDAQVKDDFINDKRKQIDREWSYRGGLVFHPTDWQSYYFSYGTSFNPSAE
                     GVALTVATNNTPPEKNEIFEVGAKLNFFGGALSFQGAVFRIDKKNARTPNPIDPLGPN
                     VVTGKQRSQGFEIGLTGRLLPGLNVFSGYTFLDTEILKDNTIPSNVGNQIQNVPKNSA
                     NVWITYDFLEKWQIGGGPSYVDSRYSNNANTNRVPGFVKWDSTVAYQITKNIQFRVNA
                     INLTNQLYFDSISGSKAIPAAGRTFLAGANFKF"
     gene            3274..>3835
                     /locus_tag="VMZ02_09855"
     CDS             3274..>3835
                     /locus_tag="VMZ02_09855"
                     /inference="COORDINATES: protein motif:HMM:NF003974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HUR72282.1"
                     /translation="MIVQVPNILNPEQIARCRESMNRATWIDGRITAGYQSAAVKDNR
                     QLPENSPEARELGEIVMAALERSPLFITAALPLRVFPPLFNRYEGGASFGAHLDNSIR
                     QIPGTPYRIRTDISATLFLSAPEDYDGGELVIDDVYGAHAVKLNAGDMVVYPASSLHH
                     VNPVTRGVRLASFFWVQSMVRDDGNRT"
BASE COUNT          916 a         1129 c         1038 g          752 t
ORIGIN      
        1 acgagcgctg gatgatctcg tcctttaccg catcatcgtt gattcatttg agcttgattc
       61 cactggcagg cctgctgata cacgccaaac cactcaagcc ggtcaacatg cccgtggagt
      121 tgatcgagct accacgcgtt gagcagccga aaaaagttga ggtcgtgcca ccgccaccgc
      181 cactaccacc ggtgccaaaa ccgaaattgc agaatgtcac cggaccgaaa ttgttatcca
      241 agccggttct tgacgccagt ccactgccgc cgaccggcaa cacaaaagaa cccgtcaaag
      301 aaacacccat cgcggaaccg cttgcagcgc tgccggataa cgccaacgcg gccaaggccg
      361 gttggaacgc cggtacgccg gccactcaag ccgagggcag taccgctggc gccggcaatc
      421 ttttcggcaa gggcgatgtc gggatagttg gcggcagcgg cgttgccggt ggcggtggcg
      481 gcaccggggc atcaggcctc ggacgcggcg ctgcaggcga cggcaccggc ggcggcgctg
      541 gctcaggaga agcgctctct ggtatggcca aacccctcgg cggctatcaa gtcaagccgc
      601 gctatcccga atccgccaaa agaattgggg ctcaaggcgt gacgacgtta cgagttcggg
      661 tactggaaac cggtcgggtg agcgaagtgt tggtcgagca aaccgccggt tttcgtgatc
      721 tcgatatcgc cgcgaccgaa gcggtcaaaa aatggctctt tgagcctgcg cgtcgaggca
      781 aagaagcggt ctcggtatgg gtcttattgc cggtcaaatt cgagttgcac taggaaacgc
      841 cgtggccgaa acacttatgc attgcgccgc caaatcaaca gcagagccgt cgatcctcgc
      901 tccggcagcg aaaaaaccgc gcgtcgaaag cgcccaccta tttcaaggca acaacgagat
      961 cgtcatcgtg catcagaagg aggaatacaa cctgcggatc accaaaaacg gcaagctcat
     1021 tttgaccaag taatattctc gatccagcca gccatccagt cttccggaca cggaaagcca
     1081 gccgatcaag caatctgact tcacggagga taggatgagt cggcaacaaa aacgcgcaat
     1141 cgcaagacaa agcgagaaga cggcatttca aaaaatctgt ctcgccgccg gcgaggggat
     1201 gagcaaagca ctggcgttca gcttcgcgct ggcgagccat gcggtggcgg ccgagcccaa
     1261 gcaggacgaa cccattaacc tgccgccggt agtcgtcgaa gaccaaagca gtccctacat
     1321 cgttccgcag tcctcgctct cgaagtttcc cgagccacta atcgacgtcc cgcaatcgat
     1381 caccatcgtg ccggagaaac ttctcgagga acaggctggt acgacattgc gcgatgcgct
     1441 gcggaacgtg cctggtatca ccgccacagc gggcgaaggc ggcggcgccc agggtgatgt
     1501 tttcacgctg cgtggcttca atgcgcgcaa cgatatgtat atcgatgggg tgcgcgactc
     1561 cggttcctat tttcgcgatt catttaattt cgactccgtc gaggtgttga agggaccgtc
     1621 ttcaacctat ttcgggcgcg gttccaccgg cggtgtgatt aatcaggtca gcaagactcc
     1681 ccggctagac tcgagctaca atggcgattt taccgccggc ggcaacatgt acttgcgcgg
     1741 caccatcgac gtcaatcaac cgatcccgca attacttccc aacgccgcct tccgtatcaa
     1801 tctgatggcc catagggacg acgtcgtgga gcgagacaag atcgaagtca agcggttcgg
     1861 tcttgctccg tcaattgcat tcggtctggg cacaccgacg caggtgacgt tgagctacct
     1921 gctgcaacac gaagacaata ttcctgatta cggattccca tacctgaatg gccgaccact
     1981 gcgcaccgat cgcagcaact tttatggatt atcaaaggac gacaacgaag atacgaccgt
     2041 gaatatcggg acactccgcg tgaatcacca attcaatgac atgttcagcc tgcacaatac
     2101 gctgcgttac agcaacgttg accgtgagtc ggcggttacc aaccccacag cagtcccccc
     2161 gaatcttaac cggatgcggc cgcaacgcga tactcaggaa agtatcttga gcaatcaaac
     2221 cgatctcacg gcaaagttct ccacctatgg cttaaaccac accgtcacga ccgggctgga
     2281 agtggcgcgc gagactttcg atttgatccg ctgggccagt gttggtccca acacaacgat
     2341 caacaatccc aacgatagtc aaccgccaag cccgaagacg ctggctgcga aatcggatac
     2401 cagcgcgtta agctttggca tctacgccgc ggaccagatc aaactcaatc agtacttcga
     2461 tctggtcggc ggtgtccgtt gggactactt cgacgcccaa gttaaggatg acttcattaa
     2521 cgataagcga aaacaaatcg acagagagtg gagttatcga ggcggcttgg tatttcaccc
     2581 gactgattgg cagagttatt atttttctta cggcacgtcg tttaatccat ccgccgaagg
     2641 tgtcgccctc actgttgcga ccaacaacac cccgccggaa aaaaacgaga tctttgaagt
     2701 cggcgccaag ttgaatttct tcggcggcgc tctgagtttc cagggcgcgg tatttcgcat
     2761 cgacaaaaag aatgcgcgca cgccgaatcc gattgatccg cttggcccca acgtcgtaac
     2821 cggtaaacag cgatcacagg gcttcgagat cgggctgacc ggccgcctcc ttccgggctt
     2881 gaatgttttc agcggttaca cctttcttga taccgagata ctcaaggata ataccattcc
     2941 gagtaatgtt ggtaaccaaa tccaaaacgt gccgaagaac agcgccaacg tttggatcac
     3001 ctacgacttc ctggaaaaat ggcagatcgg cggcggccca tcctatgtcg acagccgata
     3061 ctccaataac gccaatacca atcgcgtccc gggatttgtc aaatgggact ccacggtcgc
     3121 gtaccagatc accaagaata ttcagtttcg ggttaacgcg atcaatctga ccaaccagct
     3181 ttatttcgat agcatctccg gttcgaaggc tattccggct gccggacgca cgttcctcgc
     3241 aggagccaat ttcaagtttt aaggttcgaa aaaatgatcg tccaagttcc caatatcctg
     3301 aatcccgagc agatcgcgcg ctgccgtgaa tcgatgaatc gagcgacgtg gatcgacggc
     3361 cggataactg cggggtatca gtcagccgca gtcaaggaca accgccaatt gccggaaaac
     3421 agtccggagg caagggaact cggcgagatt gtaatggcgg cgctggagcg cagcccgctg
     3481 tttattaccg cggcgttgcc gctgcgggtc ttcccgccgc tgttcaaccg ctacgagggc
     3541 ggcgcaagct tcggcgctca tctcgataat tcgattcggc aaattcccgg gaccccgtat
     3601 cgcattcgca ccgatatatc ggcgacgctt tttctcagcg cgcccgaaga ttacgacggc
     3661 ggcgagctgg tcatcgacga tgtctacggc gcccatgccg taaagctcaa tgccggcgac
     3721 atggtggtct acccggcgag cagcctgcac cacgtcaacc cggtgacccg cggcgtgcgc
     3781 ctcgcgtcgt ttttctgggt gcagagcatg gtgcgcgacg acggcaatcg caccc
//
LOCUS       DATLCH010000335         5957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7928917, whole genome shotgun sequence.
ACCESSION   DATLCH010000335 DATLCH010000000
VERSION     DATLCH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7928917"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VMZ02_09860"
     CDS             <1..1033
                     /locus_tag="VMZ02_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72283.1"
                     /translation="ELNLNSVPAREASMMRYIRVFFLFVLTQIPQLADAQNKFLLGYS
                     SFSSNQTPLWVAKEQGLFKRYGSDPDLLLIEGGTRGAQALISGDVPIMGMSGQPVISA
                     RARGADLTMFAGMVNKMNYILVTTAAIKKPEDLKGKRIGAAQAGTASYHAVLLGFKHW
                     GLDARRDRITILPLGNQAARVGSLQSGGSDAIIVNPGLGTMLKERGNSILADFTELPI
                     PYPQQTMSIREQTLRSDPDFVERALKGIVAGNSFSLDPRNKDRVKQIIAKYLRLDRID
                     KAEEHYQSALKVLPSKPYVDVAGIASMIEFIAETDPLVAKIKPEMVINHSVLKKLDDS
                     DFIDQLSKR"
     gene            complement(1162..1851)
                     /locus_tag="VMZ02_09865"
     CDS             complement(1162..1851)
                     /locus_tag="VMZ02_09865"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR72284.1"
                     /translation="MATSQTRKLTRKELRQPDRFQVATEQALEYYRSHKNLVFAVVAA
                     LVLLGVGIWGWQIFKERQNIAAAQEFTTATELYQAEKYREALTHFDKVQQYRWSIYAG
                     LAHLYEANSYINLGELDKALNSAQRAVTASRPNTLYRQLSLMALANAAEQKNDCRQAI
                     ESYNEAHKIAAAQQSEALLGKARCLEKTGQAPAALAAYKEYLKDQPGSILSAKVAELE
                     SVKAAPAPTAK"
     gene            complement(1909..2436)
                     /locus_tag="VMZ02_09870"
     CDS             complement(1909..2436)
                     /locus_tag="VMZ02_09870"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HUR72285.1"
                     /translation="MTSKADCIEMSVGGLTLDPVTKTPIVILKDTENKLNLPIWIGLL
                     EATAMATEIEGIAMARPMTHDLLKTLLAEVGCSVQSIEITELKENTYYAAINLTLAGG
                     RQLLLDSRPSDAIALALRAKCPIYVAKAVLEASSVLQQDESKEMPVEKVSNVSQEKWG
                     EILEKMTPDDFKYKQ"
     gene            complement(2473..3798)
                     /gene="miaB"
                     /locus_tag="VMZ02_09875"
     CDS             complement(2473..3798)
                     /gene="miaB"
                     /locus_tag="VMZ02_09875"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HUR72286.1"
                     /translation="MKKLYVQTYGCQMNQYDSEKIVQVLARNNYVQTDRIDSADVILL
                     NTCSVRDKAEQKVYSALGSWKEFKNYRDDVIIGVGGCVAQQEGEKLLKRIPHLDLVFG
                     THNIHKLPEMIAQVENARARPVQTAFYRDPAYMDDGELRTHVDGIKAFVTIMQGCNKV
                     CSFCIVPHVRGREVSRPSGKILAEISSLAAHGVKEVMLLGQNVNSYGKLTPGELSFAE
                     LLARVDAIDGLRRIRFTTSHPQDLSPELTEDFARLSNLCEHLHLPVQSGSDTVLTRMR
                     RGYTKAEYLDRLYRLKKRCPKVALSTDIIVGFPGETDAEFEQTLELLRHVEYDDIYSF
                     IYSPRPQTVSAKIYNDDVPGDVKKERLKTVQALQREISLAKNRQRIGDCEEVLVEGQS
                     KLKAEQVMGRTRGNRIVNLSGSSHLIGEFVQVRITGATANSLLGEPMCA"
     gene            complement(3795..5201)
                     /locus_tag="VMZ02_09880"
     CDS             complement(3795..5201)
                     /locus_tag="VMZ02_09880"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HUR72287.1"
                     /translation="MQARVIEFANILRRNGIRVSLSENMDAFRALELIGIADPQIFRD
                     ALRTTLIKRTSDVKPFDELFDYFFLGIGQALDAIDRKIMEELGLSPEKFQEMLDQIQK
                     LLKNMEGDLSDLTKALLNNNRTELERMLREAMQQEMHNGNPETLRHTPYTRMMMRLGL
                     DRVQSEIERFRGMLQMLGENGEDLQNVMRFLDERMRDLNRLLREVIQQEQRKQGAEPR
                     DYSQRSSLADKSFSFYTEDDIRRMNDAVARLAQRLKNRLSVRRKKAARGRFDIKDTLR
                     KNMQYGGVPFHIQLDRRKKTKPQVMVLCDISDSVLNASRFMLQFVYSVQDLYAKVRSF
                     VFVAEIGEVTKLFEEHDIQTAVETALKGDVIDVFSHSNFGRAFEQFHRNFLSAVTSKT
                     TVLIIGDGRNNYNRPNDWVLREVKQKAKQLIWLNPESRMTWGIGDSEMPRYAPHCHIA
                     EECRSISQLYKIVDLIAP"
     gene            complement(5229..>5957)
                     /locus_tag="VMZ02_09885"
     CDS             complement(5229..>5957)
                     /locus_tag="VMZ02_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HUR72288.1"
                     /translation="LYEWEYAKQLLYTQILKDKIGDILQGAKTLQEAVDHVAKQDGVF
                     FSEKFLLPRPLLRALLSAKPVVLLIDEIDKSDAEFEAFLLEVLSDFQITVPEIGTLKA
                     KHIPLVVLTSNNSREVSDALKRRCLHLFLDFPDPEREKEIIKLKVPNVGDKLADEVVR
                     LLHRLRKLDLKKTPSISESLDWVRALTLLNIKDLDNELVEQTMAALMKYEADIRKAHQ
                     ELKSYLAEKQARRPTASDDKDHLH"
BASE COUNT         1274 a         1580 c         1616 g         1487 t
ORIGIN      
        1 tgaattgaat ctaaattcag tgcccgcgcg tgaggcttcg atgatgcgat acattcgagt
       61 tttttttcta ttcgtcttga cgcagattcc gcaactcgcc gacgcgcaaa ataaatttct
      121 gctgggctat tcgtccttca gttccaatca gacgccgctg tgggtcgcga aggaacaggg
      181 tctgttcaag cgctacggca gcgatcctga tttgctcttg atcgaaggcg gcacccgcgg
      241 cgcgcaggcc ctgatctcgg gagacgttcc gatcatgggt atgtcgggcc agccggtgat
      301 cagcgcgcgg gcacgcggcg cggacctgac gatgtttgcc ggcatggtca ataagatgaa
      361 ctacatcctg gtaaccacgg cagcgatcaa gaaacccgag gatctaaaag gtaaacgcat
      421 tggcgcggcc caggccggca cagcgtctta tcatgccgtg ctgcttggct ttaaacattg
      481 gggactcgac gcccggcgcg accgcatcac gattctgccc ttaggcaacc aagccgcccg
      541 cgtcggctcg ctgcaatccg gcggcagcga tgcgatcatc gtcaatccgg gtcttggaac
      601 gatgcttaag gagcgcggca attcgattct ggctgatttc accgagttgc caattccata
      661 tcctcagcag accatgagca ttcgtgagca gacccttaga agtgacccgg acttcgtcga
      721 gcgagcgctc aagggcatcg ttgccggcaa cagctttagc ttagacccgc gcaataagga
      781 cagagtcaaa cagatcatcg ccaaatattt gcgcttggat cgtatcgata aagccgaaga
      841 gcattatcag tcggctttaa aagtgctgcc gtccaagcct tatgtggatg tcgccggaat
      901 cgcttcgatg atcgaattca tcgccgaaac cgatcccctg gtggccaaga tcaaaccgga
      961 gatggttatc aatcattcgg ttctgaagaa actcgacgac agcgacttta tcgatcaact
     1021 atcgaaacga tagttcgatg ggatcgcgat taaaattaaa cgggagacga gagggcaggt
     1081 tatggcagtt acgaagacat cgaataacga actctaccga agtttacata atatatctta
     1141 tcagacgtta gctaagcgtc tttactttgc cgtcggcgcg ggcgcagctt tcacgctctc
     1201 gagttcggcg accttggcac tgagaatcga tccgggctgg tccttcagat attccttgta
     1261 agcagctaaa gctgccggcg cttgtccggt cttttccaag caccgtgctt tgccgagcaa
     1321 cgcttcggat tgctgggctg cggcaatctt atgtgcttcg ttgtaggatt ctatggcttg
     1381 acggcaatcg ttcttctgtt ccgcggcatt cgccaaagcc atcagcgaca attggcggta
     1441 cagcgtattg ggccggctcg ccgtaaccgc acgttgcgcc gagttcaagg ccttatcgag
     1501 ctcacccagg ttgatgtagc tatttgcctc ataaaggtgg gccaaaccag catagatcga
     1561 ccagcggtac tgctgaacct tatcaaaatg ggtcagcgct tcgcgatatt tttccgcttg
     1621 atagagctcc gtagcggtgg tgaattcctg agccgcggcg atgttttgtc gttccttaaa
     1681 tatttgccag ccccaaatgc ccactcccag caacacgagc gcagcgacca cggcaaaaac
     1741 taggttcttg tgggatcgat aatattccag cgcctgttcg gtggcgactt gaaaacgatc
     1801 cggctggcga agttctttac gggtaagctt tcgagtttgc gaggtagcca tgaatttgat
     1861 gtgagagcca gtgaatccta attaaagtct tagttgtgaa atgaaaactt attgtttgta
     1921 tttgaaatcg tcgggcgtca tcttctcaag aatttcgccc catttttcct gcgatacatt
     1981 ggagaccttc tccacgggca tctccttact ttcatcctgt tgcaagacac tcgaagcttc
     2041 gagcacggct ttggcgacat agatcgggca cttggctcgc agcgccagtg caatggcatc
     2101 gcttggacga gaatcaagca agagctggcg cccgccggct aaggtcaaat taatcgcggc
     2161 atagtaagta ttttctttga gctcggtgat ctcgatggac tggaccgagc agccgacttc
     2221 cgccagtagc gtcttaagca agtcgtgggt catcggccgg gccatggcga ttccctcgat
     2281 ttcagtggcc atagccgtgg cttccaggag gccaatccaa atcggcaagt tgagtttatt
     2341 ttccgtatct ttaagaatta cgattggtgt tttggtaaca ggatcaaggg ttaatccccc
     2401 caccgacatt tcgatacaat ctgcttttga ggtcattgtt tatccccttc ctactgggat
     2461 tctagcgcag gattaggcgc acatcggctc gccgagcaat gaattagcgg tggcgccggt
     2521 tatacggacc tgaacgaatt caccgattaa atggctcgaa ccgctgaggt tgacgatgcg
     2581 attgccgcga gtccgcccca tgacctgttc ggccttcagt ttggactgcc cctcgactag
     2641 cacttcttcg cagtcgccga tgcgttggcg gtttttcgcc agagatattt cacgctgtaa
     2701 ggcttgcact gtttttagac gttctttctt tacgtcgccg ggaacatcgt cgttgtatat
     2761 tttcgcggag acggtttgcg gccgcggtga atagatgaac gaataaatat catcgtactc
     2821 tacatgtcgt agcaattcta gcgtctgctc gaactccgcg tcggtctcac cgggaaaacc
     2881 aacgatgata tcggtgctga gcgcgacttt ggggcagcgc tttttcagcc gataaagccg
     2941 atccaaatac tcagccttgg tgtagccgcg gcgcatacgt gttaggacgg tatcggaacc
     3001 ggattgcacc ggtaaatgaa gatgttcgca aagattgctc aacctcgcga agtcctcggt
     3061 cagttcggga gatagatcct gcgggtggga cgtggtgaag cggatccggc gcaagccatc
     3121 gattgcatcg acccgggcaa gcagctcggc aaagctcaat tcgccgggcg tgagcttgcc
     3181 gtaggaattg acgttttggc cgagtagcat gacttccttg accccgtgcg ctgccagcga
     3241 actgatttca gcgagaatct tgccgctcgg ccggctcact tcgcgcccac gcacatgcgg
     3301 cacgatgcag aagctgcaca ccttgttgca accctgcatg attgtcacga atgctttgat
     3361 accatcgacg tgcgtgcgta gttcgccgtc gtccatatac gccggatcgc ggtagaatgc
     3421 cgtctgcacc ggccgcgccc gggcattttc cacctgtgcg atcatttccg gcagtttgtg
     3481 aatattgtgc gtgccgaaca ccaaatccag atgaggaata cgctttagca gtttctcgcc
     3541 ttcctgttgg gcgacgcagc cgcccacgcc gataatgacg tcgtcccgat aatttttgaa
     3601 ttctttccaa ctgccgagcg cgctgtagac cttttgttcg gccttgtcgc gcacgctgca
     3661 agtgttcagc agaatcacgt ccgcgctatc gatgcggtcg gtttggacat aattgttgcg
     3721 cgccagcacc tggacgatct tttcagagtc gtattggttc atctgacagc cgtaagtctg
     3781 gacgtaaagc ttcttcatgg cgcgatcaga tcgactattt tatataattg gctgatgctg
     3841 cggcactctt cggcgatgtg gcaatgggga gcgtagcgtg gcatctcgct atcgccgatt
     3901 ccccaggtca tgcggctttc cgggttgagc cagatcagtt gcttggcttt ttgtttgacc
     3961 tcgcgcagca cccagtcatt gggccgatta taattgttgc gcccgtcgcc gatgattaac
     4021 accgtagtct tgctcgtcac cgcggacaga aagttgcgat ggaactgctc gaaggcgcgg
     4081 ccgaaattcg agtgagagaa aacgtctatg acgtcgcctt tcaaagccgt ctcgacggcg
     4141 gtctgaatgt cgtgctcttc gaagagcttg gtcacttcgc caatctcggc aacgaacacg
     4201 aagctacgaa ccttcgcgta aagatcttgc accgagtaga caaactgcaa catgaagcgc
     4261 gaagcgttca aaaccgagtc ggaaatgtcg cacaatacca tcacctgtgg tttggtcttt
     4321 ttgcggcggt cgagctggat atgaaagggc acaccgccgt attgcatgtt cttacgcaaa
     4381 gtatctttga tatcgaatcg accgcgggcg gctttcttgc gccgcaccga caagcggttc
     4441 ttgagcctct gcgccaagcg cgcgacggcg tcgttcatgc gccggatgtc gtcttcggtg
     4501 taaaacgaaa agctcttgtc ggcgagcgag ctgcgttgcg agtagtcgcg cggctcggcg
     4561 ccttgcttgc gctgttcctg ctggatgacc tcgcgcaaga gccggttcaa gtcgcgcatg
     4621 cgctcatcca agaaacgcat gacgttttgc agatcctcgc cgttttcgcc gagcatttgc
     4681 agcatgccgc ggaaacgctc gatctcgctt tggacgcggt cgagccctaa gcgcatcatc
     4741 atgcgcgtat acggtgtgtg gcgcagggtc tccggattgc cgttgtgcat ttcctgctgc
     4801 atcgcttcgc gcaacatgcg ctcgagttca gtgcgattat tattgagcag cgccttggtg
     4861 agatcggata gatccccttc catgtttttc aggagtttct ggatctgatc gagcatttcc
     4921 tgaaactttt ccggcgataa cccaagctct tccatgatct tgcgatcgat ggcatcgaga
     4981 gcctggccga tacccaggaa aaaataatcg aacaattcgt cgaaaggctt aacgtcgctg
     5041 gtgcgtttga tcagggtcgt gcgcagggca tcgcgaaata tttgcgggtc cgcgatgccg
     5101 atcaattcga gggcgcgaaa cgcgtccatg ttttccgaca aggacacgcg gatgccgttg
     5161 cggcgcaaaa tattggcgaa ctctatgact cgcgcttgca tgatttttta ccgctaccgc
     5221 gcgggcggct aatgcaaatg atctttgtcg tcgctggcag tgggacgccg ggcttgtttt
     5281 tccgccaggt aacttttcag ttcctgatgc gccttgcgga tatcggcttc atatttcatt
     5341 aaagccgcca tggtctgctc gacaagttcg ttatccaggt ccttgatgtt gagcagcgtt
     5401 agggcacgca cccaatcgag gctctcactg atgctcggag tctttttcag atcgagcttg
     5461 cgcaaccggt gtaacaagcg cacgacttcg tcggcgagct tgtcgccaac gtttggcacc
     5521 tttagcttga taatctcttt ttcgcgctca ggatcgggga aatcgagaaa tagatggaga
     5581 cagcgacgtt tgagcgcgtc ggagacttcc cggctgttat tactcgtcag caccaccagc
     5641 ggtatatgct tggccttgag cgtgccgatt tccggcacgg taatttgaaa gtcgctcagc
     5701 acttcgagca ggaaagcctc aaactcggcg tccgatttat cgatctcgtc gatcaacagc
     5761 accaccggtt tcgccgataa aagcgcgcgt agcaatggcc gaggcaggag aaatttctcg
     5821 gaaaaaaata ccccatcctg tttggcgacg tggtcgactg cttcttgaag agtcttggct
     5881 ccctgcagaa tgtcgccgat cttgtctttg agaatttggg tgtagagcag ctgcttggcg
     5941 tactcccatt cgtacag
//
LOCUS       DATLCH010000336         6091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7313907, whole genome shotgun sequence.
ACCESSION   DATLCH010000336 DATLCH010000000
VERSION     DATLCH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7313907"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="VMZ02_09890"
     CDS             complement(<1..1868)
                     /locus_tag="VMZ02_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUR72289.1"
                     /translation="MDSKEVKSTPNGINRWVKSYCPYCGVGCGLLAGIANGSVAKIKG
                     DKDHPSSLGDLCLKAVYLPETLKTSDRLLYPQIRACSDGPFKRTSWEKIMGYLAQRFR
                     AILDEHGPEAVAFYGSGQLTTEEYYIANKLAKGFVRTNNFDTNSRLCMSSAVAGYSSS
                     FGSDGPPASYADFDEANCLLLVGTNIADCHPVIFKRIKQRKLSAPNDVVIICVDPRRT
                     ETADFADLHLPIRPGSDIALLNSLLYVLLDENLIDRGFIAQHTNGFNPLIDAVKDYAP
                     RVAQEICGIAECLIVEAALIFGKAERAMSLWSMGVNQSTVGVHKNNAIHNLHLATGKI
                     GKPGCVPFSLTGQPNAMGGREAGGLSHMLPGYRSVENPQHRDEVERHWGVPMGSIAPK
                     PGLAALEQFEALAEGKIKAIWIICTNPAASAPDTDFIEKALRQAELVIVQDAYHPTTT
                     SKFAHVLLPAAQWSEKEGVMTNSERRVTYMPKLTEPPGEALPDWQIIARFAAAMGFGN
                     AFAYQSAKEIFAEYAALTKSRVCDCSGMTYRKLKSTPLQWPYPKKLHRAIAHLYSEGI
                     FPTSDGRANFVAVEHAEPVERPDVAYPLVLTTGRSKYHWHTMTRTGKNPALCKSAP"
     gene            complement(1897..2856)
                     /locus_tag="VMZ02_09895"
     CDS             complement(1897..2856)
                     /locus_tag="VMZ02_09895"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR72290.1"
                     /translation="MIPALELTQLGKSYDHGRSIIVKDFNLRVSDGEFVCIIGHSGCG
                     KSTVLSIAMGLNEASEGGVIIAGREVTGPGLDRGVVFQSSALLPWLTARENVLLAVDQ
                     CQDRHSQRIRHGLADKYLLAVGLDGVTDSYPDHLSAGMRQRVGIARALALEPKVLLLD
                     EPFSLLDVVTRMELQDQVIRLCAQEKKTVLMVTHDVDEALLLSDRIVMMTNGPAATVG
                     EILGVPFDRPRDRLTLGEDPMYQHLRSAMLRFLEDHAPERAATSVQQNGEASEQPVPV
                     TAEELFIIEKYTGFNRFRGNYRKSKTPANGDFNSARREAKALA"
     gene            complement(2870..3709)
                     /locus_tag="VMZ02_09900"
     CDS             complement(2870..3709)
                     /locus_tag="VMZ02_09900"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR72291.1"
                     /translation="MAILQLKDVGKSYLRNSRAKSPTGRIEVLRDINLSIRENEFVCI
                     VGRSGSGKTTLISLIAGLIDADAGSIVLEGKPIAGPGPDRGVIFQNYSLLPWLNVFDN
                     VYLAVDAVAPELSDQEKRQRTKYYIDLVNLGPAMAKVPRELSGGMRQRVAVARGLAMD
                     PKVLLLDEPFSALDALTRGTLQEELARIWMETRKTVVMITNDIDEAILLADTIYTLTS
                     GPGATLGPAVLIDEPHPRSRLQLSGQAGYHKARRVTLKMLTGGAFAAGTVASRDATEP
                     LLQ"
     gene            complement(3720..4493)
                     /gene="ntrB"
                     /locus_tag="VMZ02_09905"
     CDS             complement(3720..4493)
                     /gene="ntrB"
                     /locus_tag="VMZ02_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HUR72292.1"
                     /translation="MEKRMDSAALVLPLLGIGLMLTIWTAISLKVAPDLPSPLKTWQE
                     SQQYILNPFFKDGEMNQGILRFALLSLIRVAKGFSLAILFGTPLGFLLGLSPKFHQAF
                     DPVIQVLRPISPLAWLPLGLVIFKQSEPAALFTIALCAMWPTVINTAMGVRSIGTEYL
                     NVGRVLKLSPMKTLRKIMIPATLPYMFTGFRLSLGMAWLVIVAAEMLTGSPGLGGFLW
                     QEYNSLVYSHILLSVLAIGLIGFALDRLMSVVERHFQRA"
     gene            complement(4508..5767)
                     /locus_tag="VMZ02_09910"
     CDS             complement(4508..5767)
                     /locus_tag="VMZ02_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HUR72293.1"
                     /translation="MKKVKKNGRREFLKVAGASSLSLLLSHLPMRWIGGVYAADAPET
                     NKIRFGIIALTDCSSIVMAHELGLFKKYGIESTISKEASWAAIRDKLTLGENQATHML
                     FGMPFASSMGLLGSPVKPMVIPFELNRNGQAITLENDLKTKGVRTPQALKPIVDEAKK
                     AGKPMTFAMTFPPGTHAMWTRYWLAAGGINPDKDVTLITIPPAQMVANMKVDKMDGFC
                     VGEPWNARAIADGIGYTAVTTQQIWKDHPEKVLAFTEEFAEKNPKTVKAILRAMTESS
                     QYIDKLENRPQMAEVVSKPQYINTQKEVILGRMLGDYDYGDGRKEKDPNYMTFFDRNT
                     NFPWKSHGVWWLTQFRRWGMVKGAPDYTGIVNKVLRPDIYREVAKEMSVTVPKEDMRK
                     ETLFDKVEFDPAKPEEYVKKFAVSNVA"
     gene            complement(5773..>6091)
                     /locus_tag="VMZ02_09915"
     CDS             complement(5773..>6091)
                     /locus_tag="VMZ02_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HUR72294.1"
                     /translation="PEEKTAARKPLIRSAEISEGTAKLVRVNNQEIAVFKHEGRLCAL
                     QNICPHEGGQLSAGWIDNGAAVCPLHGYRFDLASGACANDAKLKAKVFKLAADGENYT
                     VEE"
BASE COUNT         1298 a         1765 c         1661 g         1367 t
ORIGIN      
        1 tccggcgcgc ttttgcacag cgcggggttt tttccggttc gcgtcatggt gtgccaatga
       61 tattttgagc gcccagtggt caacaccagc ggataggcaa catccggcct ttccacgggt
      121 tcggcgtgtt cgacggcgac aaagttggcg cggccgtcag aggtcggaaa gattccttca
      181 gaatagagat gtgcaatggc tcgatgtagt ttttttgggt aaggccactg tagcggagtc
      241 gatttcagct tgcggtaagt catccccgag cagtcgcata cccggctttt ggtcagtgcc
      301 gcgtattcgg cgaagatttc ttttgccgac tgatacgcga aggcattacc aaagcccatc
      361 gccgcggcaa aacgcgcgat gatttgccaa tccggcagcg cttcgccggg cggttcggtc
      421 agtttcggca tataagtcac acggcgttcc gaattggtca tcaccccctc tttctcgctc
      481 cattgcgccg ccggtagcag cacgtgagcg aatttgctcg tcgttgtcgg gtgatatgcg
      541 tcttggacaa tcaccagctc cgcttggcgc agtgcctttt cgatgaaatc ggtgtccggt
      601 gcggacgccg cgggattggt gcagataatc cagattgctt taatttttcc ttccgccaag
      661 gcctcgaact gctccagcgc cgccagaccg ggtttgggcg caatgctgcc catcggcaca
      721 ccccaatggc gttcaacttc gtcgcggtgc tgtgggtttt ccacactgcg atagcccggc
      781 agcatatgcg acagtccgcc cgcctctctc ccgcccatcg cgttgggctg gcccgtgagt
      841 gagaacggaa cgcaacccgg tttgccgatt tttcccgttg ccaaatgcag gttatgaatt
      901 gcgttgtttt tgtgcacacc caccgtgctt tgattcaccc ccatgctcca gagcgacatg
      961 gcgcgctcgg ctttgccgaa gatcaacgcc gcttcgacaa ttagacattc ggcaatgccg
     1021 caaatctcct gggcgacgcg cggcgcgtaa tctttcacag catcgatgag cgggttaaat
     1081 ccattggtgt gctgcgcgat aaatccgcga tcgatcagat tctcgtcgag caggacgtat
     1141 agcaggctat taagcagagc gatgtcgctc cccgggcgaa tcggcaaatg caaatcggcg
     1201 aagtcagcgg tttctgtgcg ccgcggatcg acgcaaataa tcacgacatc attgggcgcg
     1261 ctcagcttgc gctgcttgat gcgtttaaag ataaccggat ggcagtctgc gatgttggtg
     1321 ccgacgagca gaagacaatt cgcctcatcg aagtcggcat acgatgcggg aggtccatcg
     1381 gaaccaaaag acgagctata gccggcgacc gccgaagaca tgcacaggcg cgagttggta
     1441 tcgaagttat tcgtgcggac gaatcccttg gcgagtttgt tggctatata gtactcctcc
     1501 gtggtgagct gtcccgaacc gtagaaagcc acagcctcgg gcccatgctc atcgagaatc
     1561 gcgcggaacc gctgcgccaa atagcccatg attttttccc aggacgtgcg cttgaaaggg
     1621 ccatcggaac aggcgcgaat ttgcggataa agcagccgat cggatgtttt taaagtctcg
     1681 ggcagataaa ccgccttaag acaaagatcg cccaggcttg acgggtggtc cttatcacct
     1741 ttgattttcg ctaccgaacc gttggcgatt cccgcgagca aaccgcaacc aactccacag
     1801 taggggcaat agcttttaac ccagcgattg attccgtttg gagtcgattt aacctctttt
     1861 gagtccaccg tatcgcacgc aacacgccgg tcgaaactac gccagcgcct tggcctcccg
     1921 gcgcgccgag ttgaaatcgc cattggccgg cgtctttgat ttgcggtaat tgccgcggaa
     1981 ccggttaaag ccggtatatt tctcgatgat gaagagttct tccgcagtga ccggcaccgg
     2041 ctgctcggag gcctcgccat tctgctgcac tgacgtggcc gcgcgttccg gcgcgtgatc
     2101 ttcgagaaag cgcagcatag cgctgcgcaa gtgctgatac attggatctt caccgagggt
     2161 cagccgatcg cgcggcctat cgaagggtac tccaagtatc tcccccaccg tcgcggccgg
     2221 cccgttagtc atcatgacga tgcgatcgga gagcagcagc gcttcgtcga cgtcatgagt
     2281 caccatcagc acggttttct tttcctgagc gcacaatcga atcacttggt cctgcagctc
     2341 catgcgtgtc accacatcga gcaagctgaa gggctcgtcg agcaacagca ctttcggctc
     2401 caaagccaaa gcgcgggcga tgccgacacg ctggcgcatg cccgcagaga gatggtccgg
     2461 gtaagaatca gtaacgccat cgagcccgac ggctaacaaa tatttatctg cgaggccgtg
     2521 acgaatacgc tgagagtggc gatcctggca ctgatcgaca gcaagtaaga cattctcccg
     2581 cgccgtgagc cagggcaaca gtgccgagga ttgaaatacc acaccgcggt ccaatcccgg
     2641 cccggtcact tcgcggccgg ctataatcac gcccccttcg ctggcttcgt tgagtcccat
     2701 cgcgatcgat aacaccgtcg acttgccaca acccgagtga ccgatgatgc agacaaattc
     2761 accgtcgcta acgcgcaggt taaaatcttt aacgatgatc gaccgaccgt ggtcgtaact
     2821 cttgcctaac tgtgtcaatt ccaacgctgg tatcatggtt ccgtgattcc tactgaagca
     2881 aaggctccgt agcgtctctc gaagccaccg ttccggcagc aaaagcgcct ccggtgagca
     2941 tcttcagcgt gacccgccga gctttgtgat accccgcttg accgctcaat tgcagtcgcg
     3001 agcgtggatg aggctcgtct atcaacaccg ccggaccgag cgtcgcgccc ggaccggaag
     3061 tcaacgtgta aatggtatcg gcgagcagaa tcgcttcatc gatgtcgttg gtgatcatca
     3121 ccaccgtctt gcgcgtttcc atccagatgc gcgccagttc ctcttgcagc gtgccgcgcg
     3181 tcaacgcatc gagcgcgcta aacggttcgt ccaagagtaa aaccttcggg tccatggcga
     3241 gaccacgcgc taccgcaacc cgttggcgca tgccgcccga tagttcgcgc ggaactttgg
     3301 ccattgctgg accgagattg accaggtcga tgtaatattt cgtgcgctgg cgtttttcct
     3361 gatctgacaa ctccggtgca acggcgtcca ctgcgagata cacgttgtca aacacgttta
     3421 gccagggcag tagagaatag ttctgaaaaa ttacaccgcg gtctggtccc ggtccggcga
     3481 tgggtttgcc ttcgagcacg atcgagcctg cgtccgcgtc aatgagccca gcgatgagcg
     3541 aaatcagcgt ggtcttcccc gaacccgagc ggccaacgat gcagacaaac tcgttctcac
     3601 gaatcgataa attgatatcg cgcaatactt cgatccggcc ggtcggggat ttcgcccggc
     3661 tgttgcgcaa ataacttttg cccacgtctt tgagttgtag aatcgccatg acgattgcct
     3721 caggcccttt gaaagtggcg ctcgacgacg ctcatcagac gatcgagggc gaagccaatc
     3781 aatccgatgg caagcaccga cagaagaatg tgcgagtaaa ctagactgtt gtactcctgc
     3841 cacagaaagc cgccgaggcc gggcgaacca gtgagcatct cggccgcgac aatcaccagc
     3901 catgccatgc cgagactaag acggaaaccg gtaaacatat atggcagcgt cgccgggatc
     3961 ataatcttcc gcagcgtctt catgggcgac aacttgagca cccggccgac attcaaatac
     4021 tcggtaccga tgctgcgcac tcccattgca gtattgatca ccgtcggcca catggcacac
     4081 aacgcgatag tgaacagcgc cgccggttcg gactgcttga agatcaccaa accaagcggc
     4141 aaccaagcca gcggagagat tggccggagc acctgaatca ccggatcgaa ggcttgatga
     4201 aactttggcg aaaggcccaa gagaaatccc aagggcgtgc cgaacaggat cgccaaacta
     4261 aaacctttgg cgacgcgaat gagactcagt aaagcaaaac ggaggatgcc ctggttcatc
     4321 tcaccgtctt tgaaaaatgg gttcaaaatg tactgctggc tttcttgcca agtcttgagc
     4381 ggcgacggca aatcgggagc gactttcaag ctgatcgccg tccaaatcgt cagcatcaaa
     4441 ccaataccga gtagcggcaa caccagcgcc gcggagtcca tgcgtttttc cattttacca
     4501 aagaccttta agcaacattg ctgacggcaa atttcttgac gtactcttcc ggcttggccg
     4561 gatcgaactc gaccttgtcg aagagcgttt ccttcctcat atcttctttc ggcaccgtca
     4621 cactcatctc tttggcgact tcgcggtaga tatccggccg aagaactttg ttgacgatac
     4681 ccgtgtaatc cggcgcgcct ttgaccatgc cccagcggcg aaactgcgtc agccaccaaa
     4741 ctccgtgaga tttccacgga aaattagtat tgcgatcgaa gaaggtcatg tagttgggat
     4801 ctttttcctt gcgcccgtcg ccatagtcgt agtcgcccag catgcggcca aggatcactt
     4861 ctttttgcgt gttgatgtac tgcggtttgg agaccacttc ggccatctgc ggccggttct
     4921 ccaatttgtc tatgtactgg ctggactccg tcatggcacg caggatcgct ttcacggttt
     4981 taggattttt ctcggcgaac tcttcagtaa aggccaggac tttttccgga tggtccttcc
     5041 agatttgttg cgtcgtcacg gcggtgtaac cgatgccatc ggcgatggcg cgggcattcc
     5101 acggctcgcc gacgcagaag ccgtccatct tgtcgacttt catattggcc accatctgcg
     5161 ccggcggaat tgtgatcagt gtcacatcct tatcgggatt gatgccgccg gcggcaagcc
     5221 agtagcgcgt ccacatcgca tgggtcccgg gcggaaatgt catcgcgaag gtcatcggct
     5281 tgccggcttt tttcgcctca tcgacaatcg gcttgagcgc ctgcggagtc cgcacccctt
     5341 tggtctttaa atcgttttcc aaagtgatcg cttggccgtt gcggttgagt tcaaacggaa
     5401 tgaccatcgg tttcaccggt gaaccgagca ggcccatgga cgaggcgaaa ggcatgccga
     5461 acagcatatg agttgcctgg ttctcgccga gcgttaactt gtcgcggatt gccgcccacg
     5521 aagcttcctt ggaaatggtc gactcgatgc cgtatttttt gaacaggcca agctcatgcg
     5581 ccatgacgat cgacgagcaa tcggtcaggg cgataatgcc gaagcgaatc ttgttcgttt
     5641 ccggcgcgtc ggcagcatac acgccgccga tccagcgcat cggcagatga ctcaataaca
     5701 gactcagcga actggcgccg gccactttga gaaattcgcg ccggccgttt ttcttgactt
     5761 tcttcatctc gatcattcct ccacggtata gttttcgccg tcagcagcga gtttgaagac
     5821 tttggcttta agcttggcgt cgttggcgca ggcgccgctg gccaaatcga atctgtagcc
     5881 gtgcagcgga caaaccgccg cgccgttgtc gatccagccg gccgacaatt gaccgccctc
     5941 atgggggcag atgttttgca gcgcgcagag tctgccctcg tgtttgaaca cggcgatttc
     6001 ttgattattc actcgcacga gtttggccgt gccctcgcta atttcagcac tgcgaatcag
     6061 cggcttgcgc gcggcagttt tttcctccgg c
//
LOCUS       DATLCH010000337         6787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7317402, whole genome shotgun sequence.
ACCESSION   DATLCH010000337 DATLCH010000000
VERSION     DATLCH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7317402"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VMZ02_09920"
     CDS             complement(<1..1111)
                     /locus_tag="VMZ02_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HUR72295.1"
                     /translation="MMHCAIVRSLALVVLLLGAGCTVGPDYRPLGVAVAPAWLEAWSN
                     GTDARPVDLVRWWTEFNDPILNKLVDRAVNANLDLAVAQARIREARGQLRAIGAEYWP
                     TLDVGGSYSNIRTSENAFAAGRVAPQGGSNLQQDLFRTGFDSSWEIDLFGGTRRRVEA
                     AQAIVDAAVEDRRSVQVTLLGDVAKNYIDVRAFQRRLAITQDNLKAQQDFLDLTIVRF
                     NAGLASDLEVAQAEGQVQTTAAQIPALESALATTVYRLDTLVGGQPGLLWNELSQPAV
                     IPALPPKAQVGLPVELLRRRPDIRRAERQLAASTALVGAATADLYPKFSLTGAFGLQS
                     ISASDWFTGQSRFWSFGPTITWPVFDAGKIRANIEI"
     gene            complement(1125..4259)
                     /locus_tag="VMZ02_09925"
     CDS             complement(1125..4259)
                     /locus_tag="VMZ02_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HUR72296.1"
                     /translation="MNFSEPFIRRPVLTTVLTLSVILFGLLSYLRLPVNDLPAVDYPV
                     IQVQADYPGASPDTVANNIATPLERQFMQINGLELVTSKSTQGHTSLTLQFALSKSID
                     AAATDVQTAISQATGSLPVDLPSPPTFSKTNPNDQPIMYIALTSDSVTRGQLYDYGST
                     QIGQRISILPGVSRVSVFGTKSAIRIKADPSAMWARGISIDDLTTAVRNGTSYTGAGQ
                     FDSPDGTTLLRPQGQLDSAQSYSQLIIGTKNDAPIYLRDVAQVRESVQDERLSMRFWA
                     RGYPVPGATVVMAVYRQAGANAVEVANSIRALLPQISAELPGSVRITPLYDRSRGIVN
                     SVNDVQETLLIAFVLVVLVIFLFLGRATDTLIPAVALPISLLLTFVAMELLGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRMERGEAALEATVQSAKEISFTILSMTISLAAV
                     FIPLVFMSGLVGRIFREFAVTIVVAIIASGLVSLTLTPLMCARLLKARGEGAKRTWME
                     RKVGGMEKRVLKSYGRSLSWFLDHRWISALIWLLCIVGTVYFFVVIPKAFLPPGDSGV
                     VTGVFIAREGSSPEQMRAIQDQVDAALMRNPNIMATIEVAGLSQFIASNQGIIFTFLK
                     PAGTRGPIQQEVAKLMADLGSIPGVIAFLRPFPVLEISTGATNQNQGQYAFAISGINP
                     DQVYESGAKLMAKLHEFPGFLTVSSDYFNNTPNLDIELRRDQAKTYGVSEARILGLLR
                     NAYAQNYVYLIKKPADQYQVILEVADSARSTPDNLALLYIRSDDGRRLVPINALVNWK
                     SSVGPQAVNHLNQFTSMTIAFNLKPGVAIGAATDFITKAAAEIVPATLRASLQGEALT
                     FRDTVRDLTLLMVLAVFVMYVILAILYESYLHPLTVLSTLPTALVGGLATLFIFREQA
                     SLYAFIGMFMLMGIVKKNGIMIVDFAMQRVEHGEAAGKAIHDASMDRFRPILMTTLAA
                     VFGAIPIALGFGADGASRRPLGLVIVGGLIVSQFITLFITPVIYLYLEDFQERVLDRV
                     GFFRRGGSVVAPTP"
     gene            complement(4256..5428)
                     /locus_tag="VMZ02_09930"
     CDS             complement(4256..5428)
                     /locus_tag="VMZ02_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HUR72297.1"
                     /translation="MTRFCRRRRWAVQISYGFALFGAGLILACGKKPETAFERPPAPV
                     TVGTAITQNVPVYIDAVGKIVAREIVSIQPQVSGRITQIHFADGADVKVGQLLFTVDP
                     RPYQAQLNQAEANLAQAEAALSLAKTNFARVENLSDPRAVSRQDYDTKKSAVQSADAL
                     VRQNRAAVDNARLNLEYCTIRSPINGRAGQRAVDVGNVVAANSTSLLVIQRLDPVYAD
                     FTVTENDLTAVQRNMTQRSLRVEVRLPDDTDDARAGQLTFLDNAVQDGTGTVKLRATL
                     PNADRRFWPGRFAKVRLILDTRVDAVLVPAEAPQLSANGTFLYVVKNDSSAEQRMVKL
                     GQRQGELVVVTEGVKAGEKVVVKGQLGVTPGGKVRIAAPPAVKQDPAAAARSGAKS"
     gene            complement(5443..5874)
                     /locus_tag="VMZ02_09935"
     CDS             complement(5443..5874)
                     /locus_tag="VMZ02_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72298.1"
                     /translation="MEFKAPSNNESGKKTAGLDERIGSLFQPDTLIGEDYASNFRRKI
                     PLEAERTLLLAILEDGIRSFQENLFATSGKRRTIFDEANEWIFSDEDSWFCSFVSICN
                     LLNLEPQYIRRGLRQWEDKMHNGAVHKKSLSVTGDEQRLVA"
     gene            complement(5932..6621)
                     /locus_tag="VMZ02_09940"
     CDS             complement(5932..6621)
                     /locus_tag="VMZ02_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HUR72299.1"
                     /translation="MSNAPRLTAEARRESIVEAVQDIFAEKGFDGTTIKELAHAAGVS
                     EALLYKHFPSKESLYHAMLDACAKGPTFAEASRILEFETSTSTLVVMVHFMISHYVLG
                     RPGDRHRAALNSLLMRSLLDDGELVRLMHKKLAAAWLKKFEACLQASAKSGELHVVPI
                     RKDICVWFVQHVAFSLMLHLRPKVPAVDYKVSREELVSQATWFALLGIGLKEDAARRY
                     YSPKALQALAS"
BASE COUNT         1574 a         1939 c         1848 g         1426 t
ORIGIN      
        1 gaatttcaat attggcgcga attttaccgg catcgaatac tggccacgtg atggtcggtc
       61 cgaagctcca gaagcggctc tgcccggtaa accagtcact ggcacttata ctctgcaggc
      121 cgaacgcccc ggttaacgaa aactttgggt aaagatctgc agtcgccgca cctaccaatg
      181 ccgtagaggc ggccaattgg cgctcagccc ggcgaatatc cggacggcgg cgcagcaatt
      241 caaccggcaa ccctacttga gctttgggtg gcaatgctgg aattacagcg ggctgcgaca
      301 attcgttcca taataaacct ggttgaccgc ccacgagagt gtcaaggcga tagaccgttg
      361 ttgccaaggc gctttccaga gccgggattt gcgccgcggt tgtctgcacc tgcccttccg
      421 cttgggccac ttccagatcg ctagctaggc cagcgttgaa gcgcactatg gtcaagtcca
      481 aaaaatcttg ctgcgccttc aagttgtctt gagtgattgc cagtcggcgc tgaaaggcgc
      541 gcacgtcgat ataattcttc gcgacatcgc caagtagtgt gacctgcacg ctacggcgat
      601 cttccaccgc tgcatcgact atcgcctgcg ctgcctcgac gcgtcggcgc gtgccgccaa
      661 ataggtcgat ttcccaactc gaatcgaaac cggtcctaaa gagatcttgt tgaagattac
      721 tgcccccttg cggcgcaact ctccccgcgg caaatgcatt ttcgctcgtg cggatattgc
      781 tgtaggaacc gccgacatcc agcgtcggcc agtattccgc gccgatcgct cggagttgcc
      841 cacgcgcttc gcgaatacga gcttgagcga ccgcgagatc caaattggcg ttgactgcgc
      901 gatcaacgag tttattaagt atcgggtcgt tgaactccgt ccaccagcgc accaaatcaa
      961 ccggacgcgc atcggtgccg ttgctccaag cttcgagcca ggccggtgca actgcgacgc
     1021 cgagcggccg ataatccggc ccgaccgtgc accccgcgcc cagcagtaaa acaacgagag
     1081 ccaagctccg gacgattgcg caatgcatca tagttacggc gtcttcatgg cgtcggtgcc
     1141 acaaccgagc caccacgacg gaaaaaccct acgcgatcca agaccctttc ctggaaatcc
     1201 tcgagatata aatagatcac cggcgtgatg aacaaagtaa taaattgcga cacgatcaaa
     1261 ccaccgacaa ttactaaacc gagcggccgc cgggacgcgc cgtcggcgcc aaaccctaat
     1321 gcgatcggaa ttgcgccgaa taccgcggct agcgtggtca tgagaatggg ccggaagcgg
     1381 tccatgctgg cgtcgtggat ggccttgcca gcggcttcgc catgctcaac acgttgcatg
     1441 gcaaaatcga cgatcatgat gccattcttt ttgacgatac ccatgagcat gaacatgccg
     1501 ataaaagcat aaagcgatgc ctgctcgcgg aagataaaga gcgttgccaa accgccaacc
     1561 aaagccgtcg gcaacgtcga tagcaccgtc aacggatgga gatagctttc gtagagaatc
     1621 gccaaaatca cgtacatgac aaataccgcc aaaaccatca gcaatgttaa atcacgaacg
     1681 gtatcgcgaa aggtcagggc ctccccctgc aagctggcgc gcaaagtcgc gggaacgatc
     1741 tccgccgcgg ctttggtgat gaagtccgtc gcagccccga tcgcaacgcc cggtttcaag
     1801 ttgaaggcaa tcgtcatgct ggtgaactga ttgagatggt tgaccgcttg cggcccaacc
     1861 gacgatttcc agttcaccaa agcattgatg gggaccagtc gcctgccgtc atccgaacgg
     1921 atataaagca gcgctagatt atccggtgtc gaacgggcgg agtccgcaac ttcgagaatc
     1981 acctggtact gatccgcggg ctttttgatc aagtacacgt agttttgcgc gtacgcattg
     2041 cgcaacaatc cgagaatgcg agcttcggaa acgccatagg tttttgcctg atcgcgacgc
     2101 agttcgatat cgagattggg cgtattgttg aaatagtcgg aagacaccgt gagaaatccc
     2161 ggaaactcat ggagttttgc catcagcttt gcgccggatt cgtagacctg atccggattg
     2221 atgccggaaa tggcaaacgc atattgccct tgattttgat tggttgcgcc ggtgctgatc
     2281 tccagtacgg gaaacggtcg cagaaaagca atgacgcccg gaatcgagcc caggtcggcc
     2341 atcagtttag cgacttcctg ttgaattggc ccccgagtac cggcgggttt cagaaatgtg
     2401 aaaatgatgc cttgattgga cgcgataaat tgactcaggc cggcaacttc gatggtggcc
     2461 ataatgttgg ggttacgcat tagcgctgca tcgacttggt cttgaatcgc acgcatctgt
     2521 tcaggcgacg aaccttcgcg agcaataaaa acaccggtta ccacaccact gtcgccggga
     2581 ggcaaaaacg ccttcggaat cacgacaaaa aagtacacgg ttccgacgat gcataagagc
     2641 caaatcaacg cagagatcca gcgatgatcg agaaaccacg aaagtgagcg gccgtaactt
     2701 ttcagcacgc gtttttccat gccgccgact tttcgttcca tccaagtgcg cttggcaccc
     2761 tcgccgcgtg ctttgagcaa gcgggcgcac atgagcggcg tcagcgttaa cgacaccaag
     2821 ccagacgcga tgatcgcaac cacaatcgtg actgcgaatt cacggaagat ccggccaacc
     2881 aggccggaca tgaagaccag cggaatgaaa actgcggcca gagaaatggt catcgacaag
     2941 atcgtaaagc taatttcctt ggcgctctgt acggtcgcct cgagcgccgc ttcgccacgc
     3001 tccatgcgcc gcaccgtgtt ttccaaaaat acgatggcat catcgaccaa aaagccgatc
     3061 gccagggtga gcgccatcaa tgacagattg tccaagctgt agccgagcag ctccatcgcg
     3121 acaaatgtca gaagtaacga gatcggcaat gcgacggcgg gaatcagcgt atcggtggct
     3181 cgaccaagga acagaaagat caccagtacg accagcacga aggcgatcaa aagcgtctct
     3241 tgaacgtcgt tgaccgagtt aacgattccc cgcgagcggt cataaagcgg cgtgatccgc
     3301 accgatccgg gcagttcggc gctgatctgc ggcagcagcg cgcggatgct attggcaacc
     3361 tcgactgcgt ttgcgcctgc ctggcggtac accgccatga cgacggtcgc tcccgggaca
     3421 ggataaccac gcgcccaaaa ccgcatgctc aaacgctcgt cttgcacgga ctcgcgcacc
     3481 tgggcgacat cgcgcaaata aatcggcgcg tcgtttttgg tgccgataat aagttggcta
     3541 taactctgcg cgctatcgag ttgtccctgc ggccgcaaca gagtggtacc gtccggacta
     3601 tcgaattgcc cagcgcccgt gtaactcgtg ccgtttctca ccgccgtggt caaatcatcg
     3661 atggaaatgc cgcgcgccca catcgccgac gggtcggctt taatgcgtat tgccgattta
     3721 gtgccgaaaa ccgagactcg ggatactccc ggcaagatgc tgatgcgctg accgatttgc
     3781 gtgctgccgt aatcataaag ttgaccgcgc gtcaccgagt cgctggtcag ggcgatgtac
     3841 atgatcggct gatcattggg attggtcttg gagaacgtcg gcggcgacgg cagatcgacc
     3901 ggcagactgc cggtggcctg cgaaatcgcg gtctgcacgt cggtagcggc ggcgtcaatg
     3961 cttttactga gagcgaactg aagtgtcagg ctggtatggc cctgggtgct cttggatgtg
     4021 accagctcca ggccgttgat ctgcatgaac tggcgctcga gcggcgttgc gatgttgttg
     4081 gcgacggtgt cggggctggc accgggatag tcggcttgaa cttgaataac cggataatcg
     4141 acggccggca aatcgttaac cggcaagcgc aggtagctca acagaccgaa tagaatcacc
     4201 gacaaggtga gaaccgttgt cagcaccggc cggcggataa acggttcgga aaagttcacg
     4261 acttcgctcc ggaccgagcc gccgccgcag ggtcctgctt tacagccgga ggagccgcga
     4321 tgcgaacctt ccctcccggt gtaacgccca attggccttt gacgacgacc ttttcgccgg
     4381 ctttgacgcc ctctgttacg accaccaatt cgccttgacg ttgaccgagc ttcaccatcc
     4441 gttgttcggc cgacgagtcg tttttgacga catagaggaa ggtgccgttt gccgagagct
     4501 ggggcgcttc ggccggcacc aaaaccgcat cgacgcgggt atcgaggatt aaacgaactt
     4561 tggcgaaccg gccgggccaa aaacgacggt ctgcgttagg cagagtggcg cgcagtttga
     4621 ctgtgccagt gccatcttgg acggcgttat cgagaaatgt aagttggccc gcgcgcgcat
     4681 cgtcagtatc gtccggcaag cggacttcga cgcgcaaact gcgctgcgtc atgttgcgct
     4741 gcacggccgt taaatcattt tccgtcacgg taaaatcggc atacacggga tccaagcgct
     4801 gaatcaccag cagtgaagtg ctgttggcgg caaccacgtt tccgacgtcg acggcacgct
     4861 gtccagcccg gccattgatc ggcgagcgaa tcgtgcagta ctcaagattg aggcgcgcat
     4921 tgtccacggc cgcgcggttc tgtctaacca aagcatcggc gctttgcacc gcgctctttt
     4981 tggtatcgta gtcctgacgc gacacggcgc gtggatcgct gagattttct acccgagcaa
     5041 aattggtttt ggcgagactg agcgccgctt cagcttgcgc cagattggcc tcagcttgat
     5101 ttagctgcgc ctgatatggc cgcgggtcga cggtaaatag cagttgaccg actttgacgt
     5161 cagcaccatc ggcgaaatga atctgcgtga ttcgcccgga tacttgcggc tgaatcgaaa
     5221 cgatttcgcg ggcgacgatt ttgcccactg catcgatata gacgggtaca ttttgcgtaa
     5281 tcgccgtgcc caccgttacc ggggctggcg gtcgctcgaa agctgtttcg ggttttttgc
     5341 cgcaagctaa aattaacccg gcgccgaaca gtgcaaatcc atacgagatt tgcaccgccc
     5401 agcgccggcg ccggcaaaat cgtgtcacgt ttacgctcgg gcttacgcca ctaaacgttg
     5461 ttcgtcacct gtgacgctta gagatttctt gtgcaccgcg ccgttgtgca ttttatcttc
     5521 ccactgccgc aggccacggc gaatgtactg cggttccaga ttgagcaagt tgcatatcga
     5581 cacaaacgag cagaaccagc tgtcctcgtc gctaaaaatc cactcgttcg cctcgtcgaa
     5641 aatcgttcgg cgtttaccgc tcgtggcaaa aagattttcc tgaaatgagc ggatgccgtc
     5701 ttccaagatc gccagtaaga gcgtgcgctc ggcttccagc ggaatcttac ggcgaaaatt
     5761 ggacgcatag tcctcgccga tcagcgtatc gggctgaaac aacgaaccga tgcgctcgtc
     5821 taacccagcg gtctttttgc cgctctcatt gttactaggt gccttgaatt ccattacccc
     5881 tccccttcaa ttaaaaaata gcttaacaat ttgacagaca aatgttaccc gtcagctagc
     5941 caacgcctgt agcgctttcg gactataata acggcgcgcc gcgtcctctt tgagtccgat
     6001 ccccagcagc gcgaaccaag tggcttggga caccaactct tcgcgcgaaa ctttgtaatc
     6061 caccgccggc actttgggcc gcaggtgcag catcagcgag aaggccacat gctggacaaa
     6121 ccaaacgcaa atatccttgc gaatcgggac gacgtgtagc tcacccgatt ttgccgaagc
     6181 ttgcagacat gcctcgaatt tcttcagcca ggcggcagcg agcttcttgt gcatcaaacg
     6241 cactaattca ccgtcgtcca gtaagctgcg catcagcaaa gagttgagcg ctgcccgatg
     6301 gcgatcgccg ggccggccga gcacatagtg ggagatcatg aaatggacca tcaccactaa
     6361 cgtcgacgtc gatgtttcaa actcaaggat gcggctcgct tcggcgaacg tcggaccttt
     6421 cgcgcaagca tcgagcatgg cgtgatatag cgattctttg ctgggaaaat gcttatagag
     6481 taacgcctcg gacactcctg ccgcgtgagc tagctctttg atagtcgtgc catcgaagcc
     6541 cttttccgcg aaaatatctt gaactgcttc aacaattgac tcgcggcgtg cctcggcagt
     6601 cagcctggga gcattgctca ttcgggttag ttagcattta ctcacttgcg ttgtcaaggg
     6661 atatttacga acttattgca gaccagctac taagtagcta actgcttaat taattgatgg
     6721 aatataccac tgcggcgcca tacaaagacg tcccgaatca acactcacca cgccgcaaat
     6781 tgtcatc
//
LOCUS       DATLCH010000338         3156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_11041360, whole genome shotgun sequence.
ACCESSION   DATLCH010000338 DATLCH010000000
VERSION     DATLCH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11041360"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VMZ02_09945"
     CDS             <1..1145
                     /locus_tag="VMZ02_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72300.1"
                     /translation="IASAISMNLLLFGVAAPISGYLIDRFGPRKVMLGSLTLLITGVS
                     GTVVMNKFWQFFLVWGVIVGLGAGGVGSVLTATVGNRWFVARRGLALGILGSASSTGQ
                     LIFLPFFMWMIANSGWRIGSTTLIIFAIILLPLLFLFMRDDPAEVGLEPYGAGQAPAG
                     GGTVGLRGMSSKNATITAKEVVTHPTFWLLCGSFFVCGGTANGLIGTHLIPHEIEIGI
                     PQIAAASLVGIMGTLNIVGTTFSGWMVDKVSPQKWLALVYALRGVSLLILPFVKSFTG
                     LVIFAIIYGLDWFATVPPSMAITADTFGRQNVGKVYGWIFMSHQIGAAIMASTAGAVR
                     DYLGEYNAAFMAGGFIAMIAAGLALQIKTKPREDTTPPVNVQPARA"
     gene            complement(1165..1569)
                     /locus_tag="VMZ02_09950"
     CDS             complement(1165..1569)
                     /locus_tag="VMZ02_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72301.1"
                     /translation="MKTKLTIRLALVTLFLIAACGKDLNTAQGIAEEFVDHHYVKIDL
                     QKAKQYTVSVAQAKIDEEIKLTSGQAIDASTQKPRVNYTLLEKNEGDQRSTYLFEGKI
                     QSDDGTSFIRKWSIATRKEANGWRVSNFTESD"
     gene            complement(1579..2331)
                     /locus_tag="VMZ02_09955"
     CDS             complement(1579..2331)
                     /locus_tag="VMZ02_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HUR72302.1"
                     /translation="MATENLTISTPHGDILSAVLHHPAQPSYGAAILCHGMESAKNSE
                     KLIFLARSLADRGVLTLRFDFRYVGASSGKFADITYSGEVEDLRAAYNFMQSRQSSKT
                     VIFGSSMGGTVALLFAAAEPSVAALVTLAAPGHPENFPKRVLTPERLKRWREQGHTIY
                     NGQRLNISLLEDLERINITEAARRIRCPVLILHGDADEVVPVAEARELHETLTSPKRL
                     SIFPNTDHRFSDPQVMRQAMTEALDWLTRHAK"
     gene            complement(2411..>3156)
                     /locus_tag="VMZ02_09960"
     CDS             complement(2411..>3156)
                     /locus_tag="VMZ02_09960"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HUR72303.1"
                     /translation="ITEQEIEKFYEDNKAQIKVEQAQVHEQIRAFLKNQKMATKREEF
                     LKSLSARAKVLVHLKAPPIQRIAVSVQGAPFKGGTNAPVTIVEFSDFHCPFCQRVVST
                     LAQLESRYGEKVKLVFRDFPIESLHPGATKAHEAARCANEQGQFWAYHDKLFASPPSS
                     NPEILKGLAKEVGLDPSTFATCLSSGKYQAAIKEDMAEGSRIGVDGTPAFFINGRQIS
                     GAQPLDGFVRVIDDELAREGSIQSEPARD"
BASE COUNT          626 a          859 c          893 g          778 t
ORIGIN      
        1 tgatcgcctc ggccatcagt atgaatctgt tgctcttcgg tgtcgctgcg ccgatcagcg
       61 gctatttgat cgaccgcttc ggaccgcgaa aagtcatgct cggcagtctg actttgttga
      121 tcactggcgt cagcggcacg gtggtcatga acaagttctg gcagtttttt ttggtctggg
      181 gcgtcatcgt cggcctcggc gccggcggag tcggttcggt attaactgcg acggtcggca
      241 accgctggtt tgtcgcgcgg cgtggcttag cgctgggaat tctcggcagc gctagttcga
      301 cgggccaact gattttcttg ccattcttca tgtggatgat cgccaactcc ggttggcgca
      361 tcggctccac gactttgatt atttttgcaa tcattttgtt gccgctgttg tttctcttta
      421 tgcgcgatga tccggccgaa gtcggcttgg agccctatgg tgccggccaa gctccggctg
      481 ggggcggcac cgtagggctg cgcggtatgt cgtcgaagaa tgccacgatt accgccaaag
      541 aagttgttac ccatcctaca ttttggcttc tatgcggtag cttttttgtg tgcggcggca
      601 cagctaacgg tttgatcggc acccatttaa tcccccatga aattgaaatc ggcattccac
      661 agatcgccgc ggcatcgttg gtcggcatta tgggcacctt gaacattgtc ggcacgacct
      721 tctccggctg gatggtcgat aaggtatcgc cacaaaaatg gttggctcta gtctatgcgc
      781 tacgcggcgt atcgctgttg attttgccct tcgtaaaaag cttcaccggt ctggtcattt
      841 ttgcgattat ttacggtctg gactggttcg cgacggtgcc accgtcgatg gcgatcaccg
      901 ccgatacctt cggcaggcaa aatgtcggca aggtatatgg ctggatattc atgtcgcatc
      961 agatcggcgc ggcaattatg gcgtcaacgg cgggagcggt gcgggattat ctcggcgagt
     1021 acaatgccgc cttcatggcg ggcgggttta ttgcaatgat agcggccgga ttggcgctgc
     1081 agatcaaaac caagcccaga gaagacacga cgccgcctgt caacgtgcag cctgctcgag
     1141 cgtgacgata gcgcaagcga gtttttagtc agattctgtg aaattcgaca cgcgccagcc
     1201 attggcctcc ttgcgcgtgg cgatggacca cttgcggata aacgatgtgc cgtcgtccga
     1261 ctggatcttg ccttcgaata gataggtcga ccgctggtcg ccttcgttct tctccaacaa
     1321 cgtgtaattc acccgcggtt tttgcgtcga cgcgtcgatc gcttgcccgc tggtcagctt
     1381 aatctcctcg tcgatcttag cctgagcgac gctcaccgta tactgcttgg ccttttgtaa
     1441 gtcgatcttc acgtaatggt gatcgacaaa ctcctcggcg atgccctgcg cggtgtttaa
     1501 atctttgccg cacgcggcga tcaggaacaa cgtgactaag gccaagcgga tcgtcagttt
     1561 agttttcatc agtgcacttt atttagcatg tctggtgagc caatccagcg cttcagtcat
     1621 cgcctggcgc atcacctgtg gatccgagaa gcggtgatcg gtatttggga agatcgacaa
     1681 ccgcttgggg ctcgtcaacg tctcatgcag ttcgcgggcc tcggcaactg gcaccacttc
     1741 gtcggcgtcg ccgtggagga ttagcaccgg acagcgaatc cggcgcgcgg cctccgttat
     1801 gttgattcgt tctagatctt cgagcagaga aatattcaaa cgctggccgt tgtaaatcgt
     1861 gtggccctgc tcgcgccaac gctttaaccg ttcgggcgta agcacgcgct tgggaaaatt
     1921 ttccggatgg cccggtgcgg cgagcgtgac cagggcagcg acactgggct cggcggcggc
     1981 gaacagcaat gccaccgtgc cgcccatgct cgagccgaag atgacggtct tgctcgactg
     2041 gcgactttgc atgaaattgt atgccgcccg aagatcttcg acttcaccac tgtaagtaat
     2101 atcggcgaac ttgccgctgg atgcgccgac gtaacggaag tcgaaacgca gagtcaaaac
     2161 gccgcgatcc gccagcgagc gcgccaaaaa aatcaatttt tcgctgtttt tcgcggactc
     2221 catgccgtgg catagaatgg ccgcgccata ggagggctgc gctggatggt ggaggactgc
     2281 actgagaatg tcgccgtgag gggtggaaat tgtgaggttc tcagtcgcca cacgggcacg
     2341 actaacacga tttcaatatg accgaaagaa tgtttcagag aagcgcaaag gcacgcctca
     2401 actacggatt ctaatctcta gcgggctcgc tttgtatcga gccctcgcgc gctaattcgt
     2461 catcgatgac acgcacgaac ccgtctaacg gttgagcgcc gctaatctgc ctgccgttaa
     2521 taaaaaacgc cggcgtgccg tcaacaccga tgcggctgcc ctccgccata tcttccttga
     2581 tcgcggcttg gtattttccg ctgctcaggc aggttgcaaa agtgctaggg tcgaggccca
     2641 cttccttggc caatcccttg aggatctcgg gattcgaact aggcggactg gcaaacagct
     2701 tgtcatgata ggcccaaaat tgaccttgct cattggcgca gcgcgcggct tcgtgagctt
     2761 ttgtagctcc cgggtgcagg ctctcgatcg ggaaatcacg aaagacaagc tttacctttt
     2821 cgccgtagcg cgactcaagc tgcgcgagcg tagaaacaac ccgttgacaa aagggacagt
     2881 gaaagtcgga gaactctaca attgtcacag gggcgttcgt gccgcccttg aaaggcgccc
     2941 cctgcaccga cactgcgata cgctggatcg gcggcgcctt gaggtggacc aagaccttgg
     3001 ctcttgcgct gagtgattta aggaactctt cacgcttggt tgccatcttt tgatttttta
     3061 aaaaagctct gatctgttcg tggacttgtg cctgctcgac ctttatttgc gccttattat
     3121 cctcgtagaa cttttcgatc tcctgctcgg tgatca
//
LOCUS       DATLCH010000339         4507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7318478, whole genome shotgun sequence.
ACCESSION   DATLCH010000339 DATLCH010000000
VERSION     DATLCH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7318478"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VMZ02_09965"
     CDS             <1..1048
                     /locus_tag="VMZ02_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HUR72304.1"
                     /translation="PQRQDIVEKFDNRWTQPGNIVTNGPFTLASWKQQNEIELRANNK
                     YFRGRPAMDRVTMYMVNEKTTALTMYEQGNLDFIDESHSIPSLDKPRLAKLPEFKLVP
                     QLRGEYYGFAVDRKPFDNPKLRRAFSLAIDRDVFPKILQGGKTPATSWIPPGMLAHNP
                     KIGLSHNPDEARRLLAEAGYSGGKGFPPIVLGYNTQEDHKLVAEAIQAMWQKTLNVVV
                     QIENQEWKVYLKKLQTDPFVVHRSGWGADYPDPDNFMKLFTSNSGNNHGRWKNPNYDQ
                     LLDQAASESDNAKRTQLYDQAQRLLTETDAAIAPLFWRAEATMLSPKFTGLEYNSMAR
                     MDLRNVKPAVSGAR"
     gene            1160..1648
                     /gene="accB"
                     /locus_tag="VMZ02_09970"
     CDS             1160..1648
                     /gene="accB"
                     /locus_tag="VMZ02_09970"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HUR72305.1"
                     /translation="MELSEDDVLHILKLIDESKFDYFQLEVGELKITVSKGDPIPLGN
                     SAQPVATGAAPAAAPAAAAKPATAPAAATAAPKAATIPPGHLAITAPLLGTFYVAPEP
                     GAPPFIQVGQQVTEDTTCGLIEVMKVFNSVRAGVKGTVVEVVAPNGGFVEFGQPLFIV
                     KP"
     gene            1728..3095
                     /gene="accC"
                     /locus_tag="VMZ02_09975"
     CDS             1728..3095
                     /gene="accC"
                     /locus_tag="VMZ02_09975"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HUR72306.1"
                     /translation="MSVTRVLVANRGEIAVRVIRACQSLGIETVVAVSDADRESMAAK
                     MANRAVCIGPPRSADSYLKVENVIAAAQGTGCDALHPGYGFLSERASLAQACVDNKIT
                     FVGPSAENITMMGDKLEARKIARSAGVPLVPGSDHARNPTEAAQLAKEIGYPLLLKAS
                     AGGGGRGIKLVWNEHEIEDTFRTAAAEARAAFGNDTLYMERYVGNARHIEVQILGDQH
                     GNVIHLGERDCSLQRRHQKIVEEAPAYAVPAEVRAQICNAAATLARSIGYQSAGTIEF
                     IYDNDSRAYYFLEMNTRIQVEHPVTEMITGVDLVAQQLRIARGEALPFTQSDISFNGH
                     AIECRITAESPQHGFRPCPGLISNWQAPTGPGIRLDTHCYSGYFVPPYYDSLLAKLIV
                     HGKNRQEAADETNRALNNFHVTGIDTLIPFLQVVIADADYRNGKVNTQWLEKKLAEYS
                     ATIAA"
     gene            3427..4344
                     /locus_tag="VMZ02_09980"
     CDS             3427..4344
                     /locus_tag="VMZ02_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR72307.1"
                     /translation="MISLLLKRLFHGLLVLWAVATLTFLLLRVAPGGPFDSERKLPPE
                     VIANLEAKYHLDEPVWLQYFRYLGAIAQGDFGPSYKYLDRGVTEIIADTLPTSIVLGA
                     LAMLFAVVVALPVGITAAYFRNSFIDRLCMSVAALGISLPNFVLGALLIWVVALQLGW
                     LQIGRWDDWSSVILPTITLGAAPTAFLASLLRSTLIESMSEEFVRTARAKGLKEVFVI
                     GKHALLHSLIPVLTVMGPLAAALLTGSFVVEFVFAIPGMGRFFITAVTDRDYPLIMGV
                     TLVYTALLVCANLVVDLLYSFVDPRIRVE"
BASE COUNT         1010 a         1278 c         1237 g          982 t
ORIGIN      
        1 tccgcagcgc caagatattg tcgaaaaatt tgacaaccgc tggacccaac ccggtaatat
       61 cgtcaccaac ggccctttca ccctggcctc gtggaagcaa cagaatgaaa tcgagctgcg
      121 cgccaacaat aaatattttc gcggcagacc ggcgatggat cgagtaacca tgtacatggt
      181 caacgagaaa accaccgcgc taaccatgta cgagcagggt aatctcgact tcatcgatga
      241 aagccatagt attcctagtc tcgacaagcc gcgtctagcg aagttgccgg agttcaaact
      301 agtgccgcag ctgcgcggcg agtattacgg ctttgcggtt gaccgcaaac ctttcgacaa
      361 cccaaaacta cggcgggcgt tttccctggc catcgatcgc gacgtgtttc cgaaaattct
      421 tcagggcggt aagactccgg caacctcttg gatacctccg ggtatgctgg cccataatcc
      481 caagatcggc ttatcgcata atccggacga ggcgcgccgg cttttggccg aggcgggcta
      541 ttcaggcggc aagggatttc cgcccatcgt tctgggctat aacactcaag aagatcataa
      601 gctggttgcg gaagcgatcc aggcgatgtg gcaaaagact ttgaacgtcg tggtacaaat
      661 cgaaaaccaa gaatggaagg tttacctcaa aaagttgcag accgatccgt ttgtggtgca
      721 tcgttcgggc tggggcgcgg attatcccga tcccgataac tttatgaaac tgttcacctc
      781 caacagcggc aataatcacg gccgctggaa gaaccccaac tacgatcaac tgctcgacca
      841 ggcggccagc gagagtgata atgccaaacg tactcaactt tatgaccagg cacagcggtt
      901 actgacggag acggacgccg cgatcgcgcc gctattctgg agagccgaag cgaccatgct
      961 cagtccgaag tttaccggcc tggaatacaa ttcgatggca cgcatggact tgcgtaatgt
     1021 aaaaccagcc gtgagtggcg cccgatagac tgtcaatttc ccagcaattt gccatccgga
     1081 gaattttcat gttaggtgcc gtgaagtgtc tcattcaacc cctgcttgat acccaacaac
     1141 ggagagaaag gtagaattca tggaactgag cgaagatgat gtcttacata tcctaaaatt
     1201 aatcgatgaa tcgaagttcg actattttca gcttgaagtc ggcgaattaa aaattaccgt
     1261 cagtaaaggc gatccgatac cccttggcaa tagcgcgcag ccagttgcca ctggcgcagc
     1321 acccgcagcc gcgcccgcgg cggcagcgaa accggcgact gcaccggccg cagcgacagc
     1381 cgcaccgaaa gccgcgacga ttcccccggg ccatctagcg attacagcgc cattgttggg
     1441 aacgttctac gtcgcccccg agccgggcgc gccgcccttc attcaagtgg gccaacaagt
     1501 cacggaagac accacctgcg gcttgatcga agtgatgaaa gtcttcaaca gtgtgcgcgc
     1561 tggcgtgaag ggcaccgttg tcgaagtcgt tgcacccaac ggcggctttg tcgaattcgg
     1621 ccaacctctg ttcatcgtca aaccgtagct gtgcgctagt tgtggggaca ggttgctttt
     1681 cgagtcatga aaagcggtct gccccccttt tttattcagg agttcccatg agcgttactc
     1741 gagtgttggt cgctaaccgc ggcgagatcg cggtgcgggt cattcgtgcc tgccagagtt
     1801 tgggaattga aactgttgtg gcggtatcgg acgctgatcg cgaaagtatg gcagcgaaaa
     1861 tggccaaccg cgctgtctgc atcggaccgc cacgctccgc cgacagttat ctcaaagtcg
     1921 aaaacgtcat agccgcggcg caaggcaccg gctgcgatgc gctgcacccg ggctacggct
     1981 ttctgtctga acgggcgagt ctagctcagg cgtgtgtgga caacaaaatt acttttgtcg
     2041 gtccgagcgc agaaaacatc acgatgatgg gcgataagct cgaagcccgt aagatcgccc
     2101 ggagcgccgg cgtgccgcta gtgcctggct ccgatcatgc gcggaatccc accgaggctg
     2161 cgcagctcgc caaggaaatc ggttaccccc tcctgctcaa agcgtccgcg ggcggcggcg
     2221 gtcgcggcat caaactggtg tggaacgagc acgagatcga agacaccttt cgcactgccg
     2281 ccgcggaagc ccgcgcggcg ttcggcaacg atacgctcta catggagcgc tatgtcggta
     2341 atgcgcgcca tatcgaagtg caaattctcg gcgaccagca cggcaacgta attcatttgg
     2401 gcgagcgcga ttgctcctta cagcggcgcc atcagaagat cgtcgaagaa gccccggcct
     2461 acgccgtgcc ggccgaagtg cgcgcacaaa tatgcaacgc cgcggccacc ctggcgcgca
     2521 gcattggcta ccagagcgcc ggcaccatag agttcatcta cgacaacgac agccgcgcct
     2581 attactttct agaaatgaac acgcgcatcc aagtcgaaca tccggtgacg gaaatgatca
     2641 ccggggtcga tctggtcgcg cagcagctgc gcattgcccg cggcgaagcg ctgccgttca
     2701 cgcaatcgga cattagtttc aatggtcacg ccattgagtg ccgcatcacg gccgagtcgc
     2761 cacagcacgg ctttcgccct tgcccgggtc tgatttcaaa ctggcaagca ccgaccgggc
     2821 cgggcattcg tctcgatacc cactgttact ccggctactt tgtgccgccc tattacgatt
     2881 ctcttttggc aaaattgatt gtgcacggga aaaaccggca ggaggccgcg gacgaaacca
     2941 atcgcgcttt aaataatttt cacgtcacag gcatcgatac gttgattcca ttcttgcaag
     3001 tagtgatcgc cgatgccgac tatcggaacg gaaaagtgaa cacgcaatgg ctggaaaaga
     3061 aactggcgga gtattccgcg acaattgcgg cttaaagcta aatcctttct accggcgaag
     3121 gcacgaatca gcacgaactg ctgatgcgcg acatcgtccg caacgacgat gtctgtgccg
     3181 tgcgcagcac cgctgcgcgc aatctgctcc tcgccttggt gaacaactta cgccgcgcaa
     3241 ggatttaacc caggttagtg tgggcaaagc cagcctcgat gtcgtcttgg aaaaagcgcc
     3301 cgcaagatcc caactgagta gtcgtaatta cctagagtct aggccgtacc cgcggaatcg
     3361 atagccagtc attttatcaa ttagacagcg ataaaattac gctcgaatga ctaacagaat
     3421 aatgccgtga tttcgttact gctcaaacgt ctctttcacg gtctgttagt tctctgggca
     3481 gtagccacgc tgacattttt gctcctgcgc gtggctccgg gcggaccttt cgacagtgaa
     3541 cggaagttgc cgccggaagt aatcgccaat ctcgaagcca aataccatct cgatgaaccg
     3601 gtgtggctgc agtactttcg ctacttgggt gccatcgcgc agggagactt cgggccatcg
     3661 tacaaatatc tcgaccgcgg cgtaaccgaa attattgccg ataccctgcc gacttccatt
     3721 gtcctcggcg ccttggccat gttgtttgcc gtggtcgtcg cattgccagt cggaatcacc
     3781 gccgcttatt tccgcaactc tttcatcgat cgcttgtgta tgtccgtcgc tgcgctcggc
     3841 atttctctgc ctaactttgt gttgggtgcg ctgctgatct gggtcgtggc gttacaacta
     3901 ggctggctac agatcggccg ctgggacgac tggtcgagcg tgattctgcc gacgataaca
     3961 ttaggcgcgg cgcccacggc ttttctcgct tcgctgttac gttcgacctt aattgaaagt
     4021 atgagcgaag agtttgtccg gaccgcccgg gccaaaggtt tgaaggaagt ttttgtaatc
     4081 ggtaagcatg ccctgctcca ttcgttaatt ccggtcttga cggtcatggg cccgctggcg
     4141 gcggcgctac tgacgggttc gttcgtcgtc gagtttgtct tcgcgatacc cggcatgggt
     4201 cgatttttta tcacggccgt taccgatcgt gactacccac tgatcatggg cgtgacgctg
     4261 gtctatacgg cgctgctggt ttgcgccaat ttggtcgtcg atctgcttta cagcttcgtt
     4321 gatccgcgca ttcgcgtcga gtagcttatg aacgccggac aaaaatcagt cgatgtcgta
     4381 attgccatga gcgccgcctt cgtcggcctg gtcgtcgtcg cggcgtgctt ggctccttgg
     4441 atcgcgccct atgggttagg cgggctggaa gaaaagcagt tgctgcagac gcccagcgca
     4501 aatcatc
//
LOCUS       DATLCH010000340        11252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7318589, whole genome shotgun sequence.
ACCESSION   DATLCH010000340 DATLCH010000000
VERSION     DATLCH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7318589"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            757..1530
                     /locus_tag="VMZ02_09985"
     CDS             757..1530
                     /locus_tag="VMZ02_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUR72308.1"
                     /translation="MQSLNRTGVKYFIGAGVTATLLMSQGCLATRDWVRENVNDPLSG
                     RITQTEGRLKEHDGQIGTLGTRMTGVEGKLGQFEGRLGQVDAKAERALSAISNLRLER
                     KIVIDMKDGANFAFNSASLPQQAQREIDGFLSDLKGDVAGADGTVFLVAGHTDSVGNE
                     EYNYELGKKRADAVSRYLITKKSMDPMKVIPVSYGQSAPVSENNNAQGRAKNRRVEIL
                     VYREGITSQAAAPAAQQPEAPRSSSSQTTQPSGEKITQR"
     gene            complement(1629..2564)
                     /locus_tag="VMZ02_09990"
     CDS             complement(1629..2564)
                     /locus_tag="VMZ02_09990"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72309.1"
                     /translation="MANKKLRIIYRSNSHAPLWLVADKSGCWQKNELEVDTSPQLIRE
                     KAVESLKSGNVDLISGNHHNLYVRNAKNGEDFVHLAQPTNNWTENKLIVRPDIRSVQD
                     LRGKKIVADKLTSHAGLNIWLFLKKEGLDVDRGDVELVELRGSSEDRWRKVVSGEYDA
                     TFVTIPHDSRAAKAGAHVISVRAIPMIRGVTLTTTTTFVKNHPQEIRLLTKGLVDAIH
                     FFITRKDETLEILKEHATPILKLQTDQEVETLYDEWAQSLEKKPYPSLEGIANVFQLA
                     VRRDPEIAGFNPLALWNTHFVRELDDSGYIDKLYQ"
     gene            complement(2640..3113)
                     /locus_tag="VMZ02_09995"
     CDS             complement(2640..3113)
                     /locus_tag="VMZ02_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72310.1"
                     /translation="MFYSWLHVIALVVYLGAVIGFWLMLLPAVAVLENHEQKVRFFVR
                     GLKFYNPLQVGSLGIILFTGAFQLTELKAAYRENFLKYLAYNLSLKLLFAFLLVIFSV
                     YQALGIGHRFVRRIEGGDEVTPEQLNAIIRRLTGANWCILLLAVITLWLGLRLHP"
     gene            complement(3126..3944)
                     /locus_tag="VMZ02_10000"
     CDS             complement(3126..3944)
                     /locus_tag="VMZ02_10000"
                     /inference="COORDINATES: protein motif:HMM:NF033683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HUR72311.1"
                     /translation="MATMIADECINCGACEPECPNNAISQGPTIFVIDPKLCTECVGF
                     HDYEACAAVCPVDVCITDPNNVESEEALIARARELHPETNFGDSFESRFRKGQGKPTA
                     SSAAGPQTAGTPASPGVAQTAVVNGSSGSAFAEADVNYVELPPIDSWNIPMRCYKCNG
                     IHEQNVKNFTIGNVIFCPHCHKSTVIRDNLNFHIRTLLKDSYDKWENEQREFIQRRER
                     ELAAFVERRAKDAAAFEAHQERERERIREQLEVIGDTYDAAGKPYKKGSRFSWG"
     gene            4316..5341
                     /locus_tag="VMZ02_10005"
     CDS             4316..5341
                     /locus_tag="VMZ02_10005"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72312.1"
                     /translation="MMIHRQVNLARIFSLAFAVGLVVLPASISRAAETPKKLRLAYAG
                     WEIGTAVAYIGVDGGLFKQYGLDIEELPIRDTLSAGVQSLLGVDLLIGFGNPLAVVQP
                     MASGGDVTVIGSHVSFDQYGMGVASSIEAVKDLKGQKVGVSALGARSDLIARVMLRRA
                     GLDPAKDVEMVAAGLAPQRALALSKNLVQGVPFREDIAAEAKKAGIKIIDVKSVPVLT
                     DLLMTTRSFIKRDEEVIRRFMKGYAAAIHYFLSKRIESLAILKKYFPGTQGVSVDAMY
                     DAYSAQLRPLPEFNNESLQAIIDVSAAADQRAGKLKPSDITDPRFFDELKNSKFLKDL
                     YIEKVSL"
     gene            5523..6269
                     /locus_tag="VMZ02_10010"
     CDS             5523..6269
                     /locus_tag="VMZ02_10010"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HUR72313.1"
                     /translation="MRLKDQVAIVTGAGRNIGEDVCKLFVAEGAKVAVVDLDPGRGSN
                     VASEINAKNPGKAISVVCDVANATSVDSMVETVVKAFGGVDILVNNAAWTDHKTLFDI
                     TEEEWDRTMNVCMRSVWYCTRAAAKVMIDQKRKGRIVNIASTSGHWGRKEATAYTTAK
                     AGVLNLTRSIAVQLAPEGIRVNSVSPNRIGSPVGQQDVPENRFVKNLAGRRGVPMDIA
                     KAVVFMVSDESEFIYAIDLPVDGGALAIRD"
     gene            6399..7433
                     /locus_tag="VMZ02_10015"
     CDS             6399..7433
                     /locus_tag="VMZ02_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HUR72314.1"
                     /translation="MSENAIQSGVKPDATLKSLPDTVLWGYIAANLPPALTIKSGQIV
                     EIEALSHQGLTTNKDPERFFAGYGIPGADVLADAKIIYSEVKRPKGASVHILTGPIYV
                     EGAEPGDTLEIRVHDIKFRVPYGVNNTGPGKGVLPKLLTEATAKLIRIDLERRVALFS
                     DDIHIPLNPFMGIMAVSPPTSLGMVSSTPPGAWGGNIDLKFTGIGSSLFLPVFNKGGQ
                     FFTGDGHAVQGDGEVDGGAIEISLKPTLQFILHKGKVTKQPRVETATDYLTTGLDVDL
                     NVAARIALQQAVEFLEQEKGLSAADAYALSSLAVDLGIGEAVDIVNLVYAKIPKNIFK
                     NNPAFWHNPA"
     gene            complement(7539..8480)
                     /locus_tag="VMZ02_10020"
     CDS             complement(7539..8480)
                     /locus_tag="VMZ02_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HUR72315.1"
                     /translation="MDSDRLILWIAFNVFVLGMLAIDLGIFHRKAHAVSLKEAGLWSG
                     IWIGLALIFNVGVYYLWGQEKALEFLTGYVIEKSLSVDNLFVFLMVFQYFNTPAQYQH
                     RILFWGIVGALVLRAIFIITGAALLSNFHWMIYVFGGFLVVTGIKMFLQGDEKIEPEK
                     NPVVRLFQRMIPVTHEYHGQNFFILKGGKRHATLMMVVLIVVETTDVIFAVDSIPAIF
                     AITTDPFIVYTSNVFAILGLRALYFMLAGIMELFVYLKVGLSFVLVFVGVKMLLVDIY
                     KIPIGASLGVIGGILLLSILASLASRRRAAATNADDG"
     gene            complement(9124..10395)
                     /locus_tag="VMZ02_10025"
     CDS             complement(9124..10395)
                     /locus_tag="VMZ02_10025"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HUR72316.1"
                     /translation="MLPREENDMLTQVSPDKPMGRLMRRYWIPALLASEIPEPDCPPV
                     RVRLLGEDLVAFRDTQGRVGLLDEHCAHRGTSLFFGRNEECGLRCIYHGWKYALDGRV
                     LDTPAEPADSTLKNKVRQNAYACQEAGGIIWTYMGPKDKQPLLPAYEWMGMPAEKIYV
                     SKSIQECSWLQGLEGECDSSHLSFLHRSFTGERQRGGGDGALYVADTAPTLEGIEMDY
                     GIRMLSCRKIAADTVYLRVSNIVMPCHGFIPTGGIKGNPEGYTIHSHVPIDDTHSMRY
                     NIHFRRNRPIEPDERQHEDEIGADFIKIRNLQNDYLIDREKQRRENFTGMGSIFLVHD
                     ACATETMGAICDRSQEHLGVSDMTVIAVRKFMLNAVRGLAAGQEPPHVIRTVEHSDVR
                     HVACIATTIPASRDPKTYVVEQLKKEKYWEA"
     gene            complement(10427..>11252)
                     /locus_tag="VMZ02_10030"
     CDS             complement(10427..>11252)
                     /locus_tag="VMZ02_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72317.1"
                     /translation="NFKDKVRQPAYKVEELAGVIFAYLGPDPAPLLPRYDVLVREDGV
                     RSLQGRIASCNFLQMVENTVDQHHFKWLHRTPNTKKWTDTVLTSDYFDYGIRDTFSRT
                     GGGERYQTVSYFIMPTLNKTGYHVPDEHPCGMAANHPGFEALRWRVPADDTHTMHFTV
                     YFTPVVNGKPVGKIPPDNAAKGVVETTPGHYRYDETTGYLARGDQDRAAQESQGEICD
                     RSIEHLGVSDKGVIMLRKLYKDSMDAIAAGKDPVGTIRDPATNRIVEITPGEFKIG"
BASE COUNT         2555 a         3013 c         3158 g         2526 t
ORIGIN      
        1 aggtcgatga gttcaagcgt gtgaagccgt cataccagca ttgaccagca tagaactcat
       61 cgggcgcagt tgtgctgtgg cttcatctgc cacaggtcga gtcgctatct actttctaat
      121 ttaccgcatt acgctcgtcg cccggcgagg ctcatgcgat tcacatcccc tgagtaccta
      181 caaataattc ttcgtggcgg gtttttcctg taagttcaag catcgactaa ctctcccgct
      241 attttggtag cgatctcatt gttgagtagc gctgtcgctc tgccgccgcc ggtttgcagc
      301 gcttatcgag aacaaagctt gtttggtttc ggcttggtat tcgccgcgcc gatcgacgtt
      361 aactgtcgtg ctgcatatgc gtgtccactt ggcgaaccaa gtacccgcgg cgctgaagcg
      421 agaacgtaaa gcgctttggc aataaccatc ggcaatcatt ttcaatacgc ttcaagccat
      481 cagttctgac ggtgagtcgt tctgctcctc aacgacgcca gacgagccga tactcgttat
      541 gaagtataac cacaacacct cacactttag ccttcaattg tgtaggataa tcccttcttt
      601 gttccttgga tattcaacaa atcccaatca aaacggcggt ttctggcatg gtacagaatt
      661 tgatatagga gcctattcgg ggaagaaaaa gactgattaa tgcctaaaac aaacgggtga
      721 gtataaacaa taacgactga gataggagga atgggtatgc aaagtttgaa tcgcacaggg
      781 gttaaatatt ttattggcgc gggggtcacg gcgacgttac tcatgagcca gggctgtttg
      841 gcgaccaggg attgggttcg cgagaacgtc aacgatcccc tgtcgggacg tattacgcaa
      901 accgagggac gcctaaaaga acatgacggt cagatcggta ctctcggcac tcgtatgacc
      961 ggagtcgaag gcaagctcgg ccaattcgaa gggcgtctcg gccaggtcga cgccaaagct
     1021 gagcgtgctc tcagtgcaat ctcgaatctt cgcttggagc gcaagattgt catcgatatg
     1081 aaggatggcg cgaatttcgc ttttaactcc gccagcctgc cgcagcaggc gcagcgtgag
     1141 atcgatggct tcttgagcga ccttaagggc gatgttgccg gcgcagacgg aacggttttc
     1201 ttggttgccg gccacaccga tagcgttggc aatgaagagt ataattacga acttggcaag
     1261 aagcgcgccg atgcggtgag ccgttatctg atcacgaaaa agagcatgga tccgatgaaa
     1321 gttataccgg tatcctacgg tcagagcgct ccggtatcgg aaaacaacaa cgcccagggg
     1381 cgggccaaga accgtcgcgt tgaaattcta gtctatcgcg aaggcatcac ttctcaggcg
     1441 gccgctccgg cagcgcaaca gccagaggca ccgcgctcat cgtcttccca gacgacacag
     1501 ccaagcggtg aaaaaatcac ccagcgttag ttccttgtaa gtagacgggg gcagaaaaaa
     1561 ggcggagtca tcgactccgc ctttttttat tgctgtctaa catggacgcg tggcgtccgg
     1621 ccccagccct attgatacag tttgtctatg taaccgctgt cgtccaactc gcgcacgaaa
     1681 tgggtgttcc atagcgccag tggattgaag ccggcgatct cggggtcgcg ccgcacggcg
     1741 agctgaaaca cgttggcaat gccttcgagt gacgggtagg gttttttctc cagtgattgc
     1801 gcccactcat cgtacaaagt ttcgacttcc tggtcggttt gcagtttcaa aatcggcgtt
     1861 gcgtgttcct tgaggatctc caacgtttcg tccttgcggg tgataaaaaa atggatcgca
     1921 tcgaccaggc ccttggtgag caggcgaatc tcttgggggt gattttttac aaatgtcgtg
     1981 gtggtcgtca gcgtgacgcc acgaatcatc ggaattgccc gcacgctgat gacatgggcg
     2041 ccggctttgg cggcgcggct gtcatgcggg atggtgacga acgtagcatc atattcgccg
     2101 ctcactacct ttctccagcg atcttcggac gagccgcgta actcgacaag ttcgacgtcg
     2161 ccgcgatcga catctagtcc ttcttttttc aaaaacagcc agatattcag gccggcgtgg
     2221 gaagttagct tgtcggcgac gattttttta cctctcaggt cctgcacgga gcggatgtct
     2281 ggccgcacta taagtttgtt ttcagtccaa ttattagtcg gctgtgccag gtgaacgaag
     2341 tcttcgccat ttttggcatt gcgcacgtag agattatgat gattgccgga aatcaaatcg
     2401 acgttgccgc tcttgagcga ctcgaccgct ttttcacgta taagttgcgg cgacgtgtcg
     2461 acctcaagct cgttcttctg ccagcaaccc gacttgtcgg cgacaagcca tagcggtgcg
     2521 tgtgaattgg agcgatatat aatacggagt tttttattag ccatagaacc ttcccgtggg
     2581 gttgtaatca gtgctacgcg cgtcatagca ctagtcagaa ctgttttgcc agtgccaaat
     2641 caggggtgaa gccgcaggcc gagccagagc gtgataacgg cgagtagtag aatgcaccag
     2701 ttggcgcccg tgagacgccg aatgatcgcg ttaagttgct ccggtgtgac ctcgtcgcca
     2761 ccctcaatcc gcctgacaaa acgatggccg atacctagag cctggtacac gctgaaaatc
     2821 accaacagaa aggcgaacag caacttaagg ctaaggttat atgccaggta cttgagaaaa
     2881 ttttcgcgat aagccgcctt caattcggtg agctggaatg ctcccgtaaa caagataatt
     2941 cccagcgagc cgacttggag cggattgtaa aacttcaagc cgcggacgaa aaatcgcacc
     3001 ttttgttcat ggttttccaa caccgcgacc gccggcagca acatcagcca aaagccgatg
     3061 actgcgccca agtagaccac cagcgcgatc acatgaagcc aagaatagaa catcgggcaa
     3121 tgggatcatc cccaactgaa gcgagagccc ttcttgtaag gcttgccggc agcatcgtag
     3181 gtgtcgccga taacctcgag ctgttcgcga atgcgttcgc gctcgcgctc ttggtgcgct
     3241 tcgaaagcgg cggcgtcttt ggcgcgccgc tcgacgaatg ccgccagctc gcgctcacgc
     3301 cgttggataa actctcgttg ctcgttttcc catttatcgt acgaatcttt gagcagggtg
     3361 cggatatgaa aattcagatt gtcccggatc accgtggatt tgtggcagtg cgggcaaaag
     3421 ataacgttgc cgatggtgaa attcttgacg ttctgttcgt gtatgccatt gcatttgtag
     3481 cagcgcatcg gaatgttcca ggagtcgatc ggtggcagct ccacgtagtt gacatcggct
     3541 tcggcgaatg cggaaccgga gctaccgttg acgacggcgg tttgtgccac acctggactg
     3601 gccggcgtgc cggccgtctg agggccagct gccgaagacg cagtgggttt cccctgtccc
     3661 ttgcgaaaac gcgactcgaa actgtcgcca aaattagttt ctggatgcag ctcccgcgcg
     3721 cgtgcgatca acgcttcttc ggactcgaca ttgttgggat cggtgatgca gacatcgacg
     3781 gggcaaaccg ccgcgcaggc ctcgtagtcg tgaaacccga cgcattccgt gcataacttt
     3841 ggatcgataa cgaaaattgt cggcccctgc gaaattgcgt tgtttggaca ctcgggctcg
     3901 caggcaccgc agttgataca ttcgtcggcg atcattgttg ccatgaacgt gaaacctcca
     3961 aaataactag cgtggaattg ccatcggcgt gaaacagcgg atatcctaaa tgaaaaaaaa
     4021 cgccgttgca aatctttgga ttggctaaca gcggaatatc agaggcattt ctcccgttac
     4081 cggcgggccc ggcacggccc acgaattcaa tgatccgccg ttacgcgctc tatttcatcg
     4141 gcacctgcgg cgcttccaac gagcgcttgc agcgacgggt ttatacgaaa tccgtcgctg
     4201 cgctcgccgg cccacagaga gtcgaatgct tccagacaac taaaatgacc gccgcgccaa
     4261 acgagttgaa ggaacgccct cttggcgcgt gcaatctcgt gagttatgat gccgcatgat
     4321 gattcaccgg caagtaaatt tagcgcgcat attttcgctg gcttttgcag tcggactagt
     4381 tgtcttgccg gctagtattt cccgtgctgc tgagactccg aagaagctgc gcttggctta
     4441 tgccggttgg gaaatcggta cggccgtcgc ttacattggt gtcgatgggg gattgttcaa
     4501 acagtatggt ctcgatatcg aagagctgcc cattcgcgac acgttgagcg cgggtgtaca
     4561 gtcgttgctc ggcgtcgatt tgctaatcgg tttcggcaat ccattggcgg tggttcaacc
     4621 gatggcgagc ggcggcgatg tcacggttat cggctcccat gtcagcttcg atcagtacgg
     4681 catgggggtc gcgtcgtcga tcgaggcggt aaaagattta aagggccaga aggtcggcgt
     4741 ctccgctctc ggtgcgcgct cggacttaat tgctcgcgtc atgttgcgcc gcgccggact
     4801 agatccggcc aaggacgtcg aaatggtggc ggctggattg gcgccgcaac gcgctttggc
     4861 attatcgaaa aacctcgtgc aaggcgtgcc gtttcgggag gatatcgccg ccgaagcgaa
     4921 gaaagccggg attaaaatta tcgacgtcaa aagcgtcccc gtgcttaccg atctgttaat
     4981 gacgacgcgc tcgtttatca agcgggacga agaggtgatt cgccgtttca tgaaaggata
     5041 tgctgcggcg atccattatt ttctttcgaa acgtatcgaa agtttggcga ttttgaaaaa
     5101 atactttcct ggcacccaag gcgtctcggt cgacgccatg tatgacgcct actccgctca
     5161 actccgcccg ctgcccgagt tcaacaacga atcactccaa gccatcattg acgtcagcgc
     5221 ggcagcagac cagcgtgccg gcaagctcaa accttccgat atcactgacc cgcgcttttt
     5281 cgacgaactc aagaacagca agtttttgaa ggacctctac atcgagaaag tgagcctgtg
     5341 agtagccgcg attaggttca catacagcgc tctcgttaac tgcgtaatat cggaatgcgg
     5401 atggcgattc tcggctagtg cctgccgcac cggtttggcg agtctccgat aagccaatct
     5461 taactggaac tttcacaacc cttttgatat gcagcagaaa atatatttag gcggaggaac
     5521 ccatgagact taaagaccag gtggcgatcg tgacgggggc gggtcgtaat atcggcgaag
     5581 atgtatgcaa gctgtttgtc gcggaaggcg ccaaggtcgc cgttgtcgat ctagatcccg
     5641 gaagaggcag caacgtcgct agtgaaatca atgcgaaaaa tcccggcaaa gcaatttccg
     5701 tcgtttgcga tgtcgccaac gcaacctcgg tcgatagcat ggtcgaaacc gtggtaaagg
     5761 catttggagg tgtcgatatc ttggtcaaca acgcagcctg gaccgaccac aaaacgctct
     5821 tcgacatcac cgaagaggag tgggaccgga cgatgaacgt gtgcatgcgc agcgtatggt
     5881 actgtacccg cgcggcagcc aaggtcatga tcgaccagaa gcgcaaaggt cgcatcgtca
     5941 atattgcatc gacgtcgggc cactgggggc gcaaagaagc gaccgcctac accaccgcca
     6001 aagccggtgt gttgaacctg acccgttcca tcgccgtgca gttggcgccc gaaggcatcc
     6061 gggtaaattc ggtctcgccc aatcgaatcg gttctccagt cgggcaacag gacgtgccgg
     6121 aaaatcgttt cgtaaaaaat ctcgctggcc ggcgcggcgt accaatggat atcgccaaag
     6181 ccgtcgtctt catggtctcc gacgagtccg agttcattta cgccatcgat ttgccggtcg
     6241 acggcggcgc cttggcgatc cgcgactagc tgataagatt tcatgtcagg gcggtggagt
     6301 cttcaacttc accgcctttt tgtttcatgg cttggcaaga tgcactgcag aagcgctgag
     6361 gcatgaagca aaaatctcca ccgcgaatag gagcaagcat gagtgaaaac gcaatccaat
     6421 ctggcgtgaa acccgacgcg acgctgaaat cgctacccga tacggttttg tggggctata
     6481 tcgcggccaa tcttccgccg gcgttgacga tcaagtcggg acagatcgtc gagatcgaag
     6541 cgctgtcgca ccagggtctt accaccaaca aagatcccga gcggtttttt gccggttacg
     6601 gcattcccgg ggctgacgtt cttgccgatg ccaagattat ttactccgaa gtgaaacgtc
     6661 cgaaaggcgc aagcgttcat atactcacgg gaccgatcta tgtagagggc gcagaacccg
     6721 gcgacacctt ggagatccgc gtgcatgata ttaaatttcg cgttccctac ggcgtcaata
     6781 ataccggacc tggcaaaggt gtccttccca aactcctgac cgaggcgaca gcgaaactga
     6841 tccgcatcga tctcgaacgg cgagtggctt tattctcgga cgacattcac ataccgctca
     6901 atccgttcat gggcatcatg gcggtatcgc cgccgaccag tttggggatg gtatcatcca
     6961 cgccaccggg tgcctggggc ggcaatatcg atctaaagtt caccggcatc ggctcgtcgt
     7021 tgttcttgcc ggtattcaac aagggcgggc aattctttac cggcgatggc catgcagtgc
     7081 agggcgacgg tgaggtggat ggcggcgcca tcgaaatctc gctcaagccg acactgcaat
     7141 tcatcctaca caagggcaag gtgaccaagc agccgcgggt cgaaaccgcg acggattatc
     7201 tgactaccgg tctcgacgtc gatcttaacg tcgccgcgcg cattgcgctg cagcaggctg
     7261 tcgaatttct cgagcaggaa aaaggtttga gcgcggcgga tgcttacgcg ctatcgagtc
     7321 tggctgtgga tttaggtatt ggcgaagcag tcgacatcgt caatcttgtc tacgctaaaa
     7381 ttcctaaaaa tattttcaaa aataatccgg cgttctggca taacccggca taacccgttc
     7441 aagattccaa gttcaagcgt tcaacgatga gtcgaagtcg ggacgaaatc gtgccgcctc
     7501 gaacaatcgc gatttcgcgg cgagtttcct taaggttcct agccgtcgtc ggcgttggtt
     7561 gcggctgcgc ggcgccggga cgccagtgat gccaggatcg agagcaagag gatcccaccg
     7621 attacgccaa gcgacgcgcc tatgggaatc ttatagatat cgaccaggag cattttgacg
     7681 ccgacaaaaa ccagcacgaa ggaaagtccc actttcaagt agacaaatag ttccatgatg
     7741 ccggccagca tgaagtagag cgcgcgcaag ccaaggatgg caaacacgtt ggacgtgtag
     7801 acgataaacg gatcggtggt gatggcgaag atcgccggta tcgaatcgac agcgaagatc
     7861 acgtcggtgg tttccacgac gatcaagacc accatcatga gcgtcgcatg tcgcttcccg
     7921 ccttttaaaa tgaaaaaatt ctgcccgtga tattcatggg tcaccggtat catgcgctgg
     7981 aacagccgca ccaccggatt tttctccggc tcgatcttct catcgccctg caagaacatt
     8041 tttatgccgg tgacgacgag aaagccgcca aacacgtaga tcatccagtg gaaattcgac
     8101 agcagagccg cgccggtgat gatgaagatc gcccgtagaa ccagcgcgcc gacaattccc
     8161 caaaataaga tgcggtgctg gtactgcgcc ggggtgttga agtactggaa gaccatcaag
     8221 aagacgaaca gattgtcaac gctgagagat ttttcgatga cgtaaccggt caggaattcg
     8281 agcgcctttt cttgccccca gaggtaataa acgccgacgt tgaaaatcaa cgccaggccg
     8341 atccaaatac cactccaaag gccggcttct ttgagcgaaa ccgcatgggc tttgcgatga
     8401 aaaatgccga ggtcgatggc gagcatacca agcacgaaaa cattgaaagc tatccagaga
     8461 attaaacgat ccgaatccat taggtctccg ttccagaaaa aggctagagc agcacaggcg
     8521 cgtgccggaa caaacgggaa cgagaagaaa gcctaaatac gaatcacgcc gtggcgttga
     8581 tgcagatcag gcggagagct ggacgagcgc gccagtgata gcgcgtgcgg agaaacagat
     8641 tgcaccgcgg ccgtgatgat agtgaaactt cccggaagaa tagctcggcg gtgcttgccg
     8701 gcgactttcg gcgctgaacg cgacaccgca gagcgcgcct gcgcttcttc ccgttcaagg
     8761 ggagtctcag gatcagtcga gcgctcgctc acttcgtgca cggtgaccgt acgcgcgagc
     8821 gcgaggctga aaatcaaccc aagaataaga aggcaattga aaactatccg gcccacggca
     8881 aatacgggta aacatttcgc tgggtcaagt caatatgtcg cggcgcgaca cttggatcga
     8941 tccggcatga atttccctcg cgatagcgtc gaatgcccag ttgtggaatc atagtgttag
     9001 tcgggcagca ggcaagaaac tgtacgctcc gcctgaaatg acgggcttgg gccgtcaact
     9061 ttgcgtcttc gccgcctata cgaaagttaa tccgattctt agctgccttc cttgcggcta
     9121 attctatgct tcccagtact tctctttttt caactgctca accacgtagg tcttcgggtc
     9181 tcggctcgcc ggaattgtcg tggcgatgca ggcgacgtgg cgcacatctg agtgctcgac
     9241 ggtgcggatc acgtggggcg gttcctggcc ggcggcgagg ccgcggacgg cgttcaacat
     9301 gaacttgcgc acggcgatca ccgtcatgtc gctcacgccg agatgttctt gcgaacggtc
     9361 gcagatcgct cccatggttt ccgtagcaca ggcgtcgtgc accaaaaaga tcgatcccat
     9421 gccggtgaag ttttcccgcc gctgtttttc gcgatcgatt aaataatcat tctgcagatt
     9481 acgaattttg atgaagtcgg cgccgatctc gtcttcgtgc tgccgttcat ccggctcgat
     9541 cggccggttg cggcgaaagt gaatgttgta gcgcatgctg tgcgtgtcat cgatgggcac
     9601 atgggagtgg atcgtgtagc cttccggatt acccttgatg ccgcccgtgg gaataaagcc
     9661 gtggcagggc atgacgatat tcgagacgcg cagataaacg gtgtcggcag cgatcttgcg
     9721 gcaagaaagc atgcggatgc cgtagtccat ctcgatgcct tccagtgttg gcgcggtgtc
     9781 ggccacgtac agcgccccat caccgccgcc gcgctgccgt tcaccggtga aagaccggtg
     9841 caggaacgac aagtgggagg aatcgcattc gccttctaaa ccttgcaacc aactgcactc
     9901 ttggatcgat ttgctgacgt agattttttc cgccggcatg cccatccatt cataagccgg
     9961 caagagcggc tgcttgtcct tgggtcccat ataggtccaa atgatgccgc cggcttcctg
    10021 acatgcatag gcgttttgcc ggactttatt tttcagcgtg ctatctgcgg gctccgccgg
    10081 ggtgtcgaga accctgccgt cgagagcgta tttccaacca tgatagatgc agcgcaggcc
    10141 gcactcttca ttgcggccaa aaaacaacga cgtgccacgg tgggcgcaat gctcatcgag
    10201 taggccgact ctgccttggg tgtcgcgaaa ggccacgaga tcttcaccga gcaaacgcac
    10261 gcgcaccggc ggacagtcag gttcgggaat ttcgctggcg agcaacgccg gaatccaata
    10321 acgccgcatg agtcggccca tcggtttgtc cgggcttact tgggtcagca tgtcgttttc
    10381 ttcgcgtggc agcatggctt ggtcttcttt cagagcgagt tattgtttag ccgattttga
    10441 attctcccgg cgtgatttcg acaatgcgat ttgtcgccgg atcgcggatc gtgccgacgg
    10501 ggtctttacc ggcagcgatg gcgtccatcg agtctttgta gagtttgcgc aacatgatca
    10561 cgcccttgtc cgacacgccc aaatgttcga tgctgcggtc gcaaatctcc ccttgacttt
    10621 cctgggcggc gcggtcttga tcgccgcgcg ccagatatcc cgttgtttcg tcgtaacggt
    10681 agtgccccgg cgtggtttcg accacgcctt tagcggcgtt gtccggcggg atcttgccca
    10741 cgggtttgcc gttcaccacg ggcgtgaagt agacggtgaa gtgcatcgtg tgggtgtcgt
    10801 ccgccggcac gcgccaacgc agggcttcaa agccggggtg attggccgcc atgccgcagg
    10861 gatgttcgtc cggcacatga tagccggtct tgttgagtgt cggcatgatg aaatagctga
    10921 cggtctgata gcgctcgccg ccgcccgtgc gcgagaaggt gtcgcggatg ccataatcga
    10981 agtagtcgga cgtcagcacc gtgtccgtcc actttttggt gttgggcgtg cggtgcagcc
    11041 acttaaagtg atgttgatcg accgtattct cgaccatctg caagaaattg cagctggcaa
    11101 tgcgcccctg cagcgagcgc acgccgtctt cgcgcaccag cacatcgtag cgtggcaaaa
    11161 gcggcgccgg atcaggcccg agataagcga aaatgacgcc ggccaattct tctactttgt
    11221 aagccggctg gcgcactttg tctttgaaat tt
//
LOCUS       DATLCH010000341        19031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3616660, whole genome shotgun sequence.
ACCESSION   DATLCH010000341 DATLCH010000000
VERSION     DATLCH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 19031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19031
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3616660"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="VMZ02_10035"
     CDS             <1..1739
                     /locus_tag="VMZ02_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HUR72318.1"
                     /translation="ALYILMHELPLEATPFGPENKVVMMTGPLTGNGFTPGGTKMTAV
                     YLSPLTNTTLGRGATSGYWATYLKAAGYDGVIVEGVSAKPVYLYIKDGKAELRDGAKV
                     WGLGTRDTEDRLREIVGNRDARVLCIGPAGENMNRGAMLANDYNHFAAHSGGAVLGSK
                     KLKAIVVSGTVRPSYRDKVKLADAGLRWRNVLQQHDVKKKKTKYGHGDAWGALNNLNW
                     RSTDIQPAHIAGFAENDVVLRPCFQCARMCPWDAKLGEGEFKGTVVHFNAGGEWLDTF
                     FNLGIKGNAVLYLAEKINNLGIECSHFSCGAGVAFEAWEKGLLGPDRTNGIKLEWGNV
                     DAVSKLLDMAAHREGWLGNLLAEGPKQLAEALGGDAPNWAVHTKGGTPAMHEWRPLLG
                     QMLRELVASGGMKPQGGGSKNPPPDLRDNEHWGPLEMDNPEGWVWSHVLSEQYRQFTG
                     IFGGCWFAQESHKKDGLNSIVDAFNAITGWNFSMEEALQAGHRSMILQSLFGTQRGWV
                     ADFDWQDVGPRFLEPIPDGKYKGFTIAKWLPDMIWDYYKQSGRHERTGRPFKDTLEKL
                     GLEEFQQWSQLD"
     gene            complement(1750..2490)
                     /locus_tag="VMZ02_10040"
     CDS             complement(1750..2490)
                     /locus_tag="VMZ02_10040"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72319.1"
                     /translation="MASGTWRGIVGVVKPTYGSGSLVEFIRLLPEGVGCIPMYAGIKE
                     HSERGYLGAMETYHAKVAELAEIGVDLMHPEGGPPFMVRGHQAEQEIVGAWEAKYKVP
                     IFTSGMSQAQALRALGIKKFVGCTYFRDQKMNDVFTRYFTDCGFEVLGMEGMDTSPSE
                     ADEISNETIYRHLKASFNKHPKAQGIYLLGSGAWGIKDIVTAENDLGVPVVHPVAARV
                     WYVQKLLKIKNPTIGASRLLEELPPFAG"
     gene            2681..3487
                     /locus_tag="VMZ02_10045"
     CDS             2681..3487
                     /locus_tag="VMZ02_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HUR72320.1"
                     /translation="MAIKPTEVLTCEYGNLVPTGRLLDGKPRSILFDATKCIGCRHCV
                     QACKDWNDHDRTSLYQLSSTNWITMEPPVLEGLSPIWARNSCMHCDFAACAAVCPVEA
                     ITKYEEGAVVIDKDVCIGCEYCIHACPWEVITKNEITGKASKCTMCSDRISEDKQPFC
                     VQACPVDALDFGFTEEIADKAKKQANAIGGYTYGDKEAGGGSVVYVLKEGKDKYGLRD
                     IAAGRFPKHRIPLGLMLKDLFTPRCGVAGKMRALWLAITHPKRLIYRYFS"
     gene            3673..4647
                     /locus_tag="VMZ02_10050"
     CDS             3673..4647
                     /locus_tag="VMZ02_10050"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72321.1"
                     /translation="MAAILSVIVVGIGTHAQAEKIRTAIPQANLNYLSIYVADGRGFF
                     KDEGLDNETVVISGPLATAALLSGDVDYSGGGGSGMRAAIKGAPIKGIYFQSEKVTFY
                     LVAEPSIKSAADLKGKRVAVGSSGDTQDRMITMFIERGGVSSREIVRISVGSDTATRI
                     LAIKSGNAHATTVDPGGLVFAQKEGLVSLGFLGDLFPMPFQGFVATDRKIRDNPSQVK
                     RWLRAAIRGLMFVRDRPEEAVDLGMKKLQLGRATKAMIVEGTKNYVRALPQGVPGMPS
                     AEGIKNFLEYDIKIPLGLKEDIPPERLLHLQLVKEVKEEVEMAQRRTK"
     gene            4648..5376
                     /locus_tag="VMZ02_10055"
     CDS             4648..5376
                     /locus_tag="VMZ02_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR72322.1"
                     /translation="MATEQQLREQLATCTRIFAMQGLIGLFGHVSVFQPESKTVLVTP
                     GSGSDKAHFSASDMIPIDLSGKPINGTIPPPVEWPIHTALHAARSDALAVAHLHTPYA
                     TLFAIAKREFRPVTLQGTVFGEGVPLYKEAHLVTTPQRGEAMLKTIGNNRALLMRGHG
                     IVVVGRTLAEVLFASLILEDDAKKTMQAAPLGPVEHFSAEECKSFNAEIDFERRANRA
                     WEFFSRIESQWDHQGGTGKIELFP"
     gene            5480..6535
                     /locus_tag="VMZ02_10060"
     CDS             5480..6535
                     /locus_tag="VMZ02_10060"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HUR72323.1"
                     /translation="MSEGVRLYAGTQHGLITWKSTNAGWQEVARHFEDGIIDSIHGCK
                     QAPEKVFVGVTHDGLYRTSDGGNSWAKVFEGDIRSVSVDPSNDSVVYTGIEPVGLFRS
                     EDGGDSWQEVTALKKLPQSVRKNWWYPQAPHHGHVRNIHIHPDNPQTIYLCLEHGGIV
                     RTFDRGATWEDVSKGIDYLDIHVIANFPKRYDRYYVASARGFFTSDTPETGWTRAENG
                     MTRDYFHDFLFLNPESESEPATLLIGAADASPGFWRRENRGARAAIFKSTDGAQSWSR
                     VTNGLAEELDSMVWALVHHPTDHKSVFAGFGNVARGHASGAGGAGDLMISRDKGDSWE
                     RLDIDLPADRVLWAAAE"
     gene            6663..8570
                     /locus_tag="VMZ02_10065"
     CDS             6663..8570
                     /locus_tag="VMZ02_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HUR72324.1"
                     /translation="MANSDYTNFFPTTRNTRLPGGYMGKILRVDMTTGKLTDVNLPEE
                     ALLRKYWGGQLFAEYVLLNELPLDIDPYDSRNVIVGMTGPITGTGFTPGGTKMCFVYL
                     SPATKYTLGRGATSGYFGTSLKNAGYDGIIVTGAALTPKYLYIGEDKVELRDASHVWG
                     KGIRETEDTLRRDSGHQDARIGAIGPAGENLVRAAMLVNDYNHTAAHGLGAVMGSKKL
                     KAVVAWGTKRPRLHNKNNLIDAGVRWAKAIASRKTTVKKRLTSVGHGEDWGAITKLNW
                     RSTIITDEARGLDQNRVTLRPCFQCPRMCPWDVEIGEGRFKGTIGHFNAGSEWMDTFY
                     NLGFKGNDVLYLSERINDLGIECSHYACGAGLAFEAWEKGVLGPDKTDGLNLEWGNLE
                     AVKTLLERCARRETWLGNILADGPKELAEELGGEAKNWVVHTKGGTPAQHEWRPMLSQ
                     MLRELVASGGMKPQGAGGKEPPDDLKYREKWGPLDPNQPDGWTSSHLITEKVRQACGI
                     MGGCWFALNDKPRDGVQSMLDALTATTGWDVTLDELIDAGHRAIILQSVFGTQRGWIA
                     EHDWQDVGPRFLEPIPDGKYQGFTIAKWLPGLVYEYYKISGRHEHTGRPFMDTLQKLG
                     IEEFKEWSQLD"
     gene            8593..9582
                     /locus_tag="VMZ02_10070"
     CDS             8593..9582
                     /locus_tag="VMZ02_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72325.1"
                     /translation="MRVRKFLILSLVTLLVATYFEPRAYAQALKKIRMASSSTNVSFL
                     ALYTALHRGFFKDEGIDLEIIFMPANLASTAVLNGDVDFNGAVTGTIGAAVQGRPMKV
                     LLFTVSKPLLFLMGQKNIKDLKQLKGKKIAGSSPGGSATLLANQALKQIGLEPGKDVS
                     VLQMSGNAASRLAVLESGVVEASLISVPENIIAQEKGFNELLFLGDVIEFPQNGFGTS
                     DKRIRENPDEVYRMVRATLRGLQFIWDKNNSEAVTNILMKQWKVSDRKMAAEMSRQVG
                     RVLTKDAYVKPESVQVLIDLARESAKVSKSVAVAEVVDYSFLDKARKELGMIK"
     gene            complement(9620..10645)
                     /locus_tag="VMZ02_10075"
     CDS             complement(9620..10645)
                     /locus_tag="VMZ02_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent dehydrogenase"
                     /protein_id="HUR72326.1"
                     /translation="MRVAMYYNNRDVRLEELPVPAIGPGELLLRTRASGVCGSDLMEW
                     YRIKKAPLVLGHEITAEVVEIGAGVAGYKVGDRVFSTHHVSCGTCRYCKTGHQSVCDL
                     LRTTHFEPGGFAEYIRVPKINVELGTLRIPDAMTFDEGSFIEPLACVVRAQRFAKLAT
                     GQTVLVIGSGISGLLHIQLAKARGADRVIATDISEYRLNAARQFGADATINGAQNVPD
                     RLKDLNGGRLADLVIVCTSAMAAITQATKSVDRGGTLLLFAPTAAGVDVPIPLFDFWR
                     DEINVVTSYAGSGDDLKESLALIRDHKVRVADMVTHRLPLAETGRGFQLTASGQDSIK
                     VIIDPLL"
     gene            complement(10684..11487)
                     /gene="lsrF"
                     /locus_tag="VMZ02_10080"
     CDS             complement(10684..11487)
                     /gene="lsrF"
                     /locus_tag="VMZ02_10080"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HUR72327.1"
                     /translation="MSMHWGMANRLARIIKADGRAVMLAVDHGYFLGPTSGLEEPRKT
                     IEPLMPYADSIMLTRGVLRTSVDASADKPIVLRVSGGTSILKELSNEEITVSIEECIR
                     LNVSAIALSIFVGSEFEKETLVSLAKLVDQGEKYGIPVLAVTAVGKDMARDVRYLGLS
                     CRIAAEMGAHIVKTYHCDNFEELIGSCPVPVIVAGGKKTPELEALELTYNAVKGGAVG
                     VDMGRNIFQSDSPVGMIKAVRAIVHENISVKEALKIYESEKNSQARKAS"
     gene            11621..12040
                     /locus_tag="VMZ02_10085"
     CDS             11621..12040
                     /locus_tag="VMZ02_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR72328.1"
                     /translation="MAIEIVDMVKKAGEGKRQVIFNTHRFHAWVHVYPKPGDADDMHC
                     HNADQTFYVIDGECTMHFPDGGKAVMTPGQVATITGGSFYQLENTGTGPMVLMGNRSG
                     PSEAIQHINYELRKDIKTLSKEEMDKIRHPGNVRVAG"
     gene            complement(12050..12943)
                     /locus_tag="VMZ02_10090"
     CDS             complement(12050..12943)
                     /locus_tag="VMZ02_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUR72329.1"
                     /translation="MPYVASKFASKLQLERGRLFILAAAILWSLGGVFIKFLDIHPLA
                     IVFYRSLFAAIVFTPFLKRYDFRYNRRIIFSVLSYTAAISAFVSANKLTSAANAIVLQ
                     YTAPIFVFLFSWLVMREQIARPNIFALGVAMIGIGIISFDSAGEPEMAGVALALLSGL
                     LFAGYMINLQRINNVAPVYLTWVNNLVCAFLIFGIVQSHLTLTTTQIVILVIMGAVQL
                     GAPYFLFSKGLRSVSLQEASLIALVEPVLNPLWVALIAGEFPSTATVWGGAMILFGLG
                     VRYLWPLIGAHAHAKAINQTS"
     gene            complement(13089..13541)
                     /gene="rplI"
                     /locus_tag="VMZ02_10095"
     CDS             complement(13089..13541)
                     /gene="rplI"
                     /locus_tag="VMZ02_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HUR72330.1"
                     /translation="MEVILKEDIANVGKIGEVVRVRDGYARNFLLPRGLVLEANKKNL
                     KSFDHSKKIISDQKQKVLRQAQAVGDELSGVSLVIPMKVGEEGKLFGSVTNMQIDKAL
                     KAKGLVVDRRKIQLNETIKVAGEYEVPVRLSADLTVMLKVSVVSDSKD"
     gene            complement(13552..14505)
                     /locus_tag="VMZ02_10100"
     CDS             complement(13552..14505)
                     /locus_tag="VMZ02_10100"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HUR72331.1"
                     /translation="MQRLEVITRFVLALALSVFLFISGISVPPIGALLLPFVIQPVLL
                     FGFKYGVGYGLAVVLVATLLLALFAAEELSLLYGIFAILAAVLFALVGRLRMVEYVVV
                     GAASAMLVWSLGVARYFFGSWSALAADFRGGLTQQFAAATRMQEQLGFSKESLELLKE
                     RMPQLIDMTLQLLPGLMFLTFAFIALINLLSLRRRFPEHRARWFAIENFRDWKGPEAL
                     VWGLIACGFSLFFPVPDLVRVIAMNVLLIVGAFYFAQGLAVIAFFFHKNNVPRFLRGI
                     TYILIVFQQIFTLMVVGLGLFDLWGDFRRLGKDNLTPSRAS"
     gene            complement(14527..14742)
                     /gene="rpsR"
                     /locus_tag="VMZ02_10105"
     CDS             complement(14527..14742)
                     /gene="rpsR"
                     /locus_tag="VMZ02_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HUR72332.1"
                     /translation="MFRRKVCRFCADKSLRIDYKDLRTISSFVTERGKMTPSRITGNC
                     ARHQRLLTTAVKRARSIALLPFTTANN"
     gene            complement(14967..15410)
                     /gene="rpsF"
                     /locus_tag="VMZ02_10110"
     CDS             complement(14967..15410)
                     /gene="rpsF"
                     /locus_tag="VMZ02_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HUR72333.1"
                     /translation="MDNFLAEDNHTVTLYETLFVVHPEKGSRNKEFIERFNKVVEGQE
                     GSIVQVEEWGLRDLAYRIEKQGKGFYTLMRYKATGKTVAELERNLKLTDGILRYLTTR
                     AEEDSPMTSPSAPRAYSDEPPRRNYDDRRNYDDNRSRGGDRDPSA"
     gene            complement(15450..16016)
                     /gene="pth"
                     /locus_tag="VMZ02_10115"
     CDS             complement(15450..16016)
                     /gene="pth"
                     /locus_tag="VMZ02_10115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HUR72334.1"
                     /translation="MKIVVGLGNPGNKYDATRHNIGFLFIDRVARQQGIVLDHYQCDA
                     LAGKGRVNETTLLLAKPQTFMNRSGVAVAALLQEQGASPADLIVVHDDLDLPFGRIRI
                     RTNGGAGGHRGVMSIIEHLGNAAFCRLRLGIGRPPEDLEVIEYVLAPFNESELGDLTK
                     LMDRATEALCCLLDKGAEAAMAIYNRVD"
     gene            complement(16052..16696)
                     /locus_tag="VMZ02_10120"
     CDS             complement(16052..16696)
                     /locus_tag="VMZ02_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HUR72335.1"
                     /translation="METLEIQVEARDKQRKRDAKRLLRSGKIPAILYGPKMAAVGLAL
                     DKKEFSRRVAGLQGSHLVRLKSGAATLAEKVALVKDMQFHPITGDVIHADFYEVDLTA
                     KIQVHVPLHFVGKAVGTARGGILQPIVREIEVECLPLDIPEFFDVDVSALDIGDSVHI
                     EDLQMPEGVVSLSEDNLALVTVVPPTVEEAPTTAAPAAEAAPVAAPDAKKDSAS"
     gene            complement(16714..17664)
                     /locus_tag="VMZ02_10125"
     CDS             complement(16714..17664)
                     /locus_tag="VMZ02_10125"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HUR72336.1"
                     /translation="MGRTADLKIFAGNSNPPLAAEISQYLKVPLGKATVDTFSDGESK
                     VEIKENVRGADVFVVQSTSAPGNNNLMELLLMLDAFKRASAQRITAVMPYYGYARQDR
                     KVVPRVPISAKLVADLITRAGASRMLTMDLHSGQIQGFFDIPVDNVYATPVLLDYLRK
                     QLNHNEVTIVSPDAGGVERARAIATRLDASLAIIDKRRVGPNVVAEMNIIGEVQDRIA
                     ILVDDMVDTAGTLTMAAVALKKEGAKNIIGCCSHAVLSGPAIKRIEESPLEQLIVTNT
                     IPLGPHAQGCSKIKILSVAHLIGEAIRRTHEEESISSLFV"
     gene            complement(17808..17881)
                     /locus_tag="VMZ02_10130"
     tRNA            complement(17808..17881)
                     /locus_tag="VMZ02_10130"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17847..17849),aa:Gln,seq:ttg)
     gene            complement(17929..18216)
                     /gene="spoVG"
                     /locus_tag="VMZ02_10135"
     CDS             complement(17929..18216)
                     /gene="spoVG"
                     /locus_tag="VMZ02_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HUR72337.1"
                     /translation="MQVTEVRVFPVEEDKLRAYVTITLDHCFVIRDLKIIHGATGLFV
                     SMPSKKRKDGTYKDIAHPINNETRRMLEEKIIAEYQKILADGVTPTSVHDD"
     gene            complement(18285..>19031)
                     /gene="ispE"
                     /locus_tag="VMZ02_10140"
     CDS             complement(18285..>19031)
                     /gene="ispE"
                     /locus_tag="VMZ02_10140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HUR72338.1"
                     /translation="RQIRRITSQQSEIPLLIKIRCNHPEVPSGRDNIVYRAAELLMRK
                     SKIRQPVVIHIQKKIPVGAGLGGGSTDAAAVLIGLNRIWKLRLSVRQLERLALQLGAD
                     VPFFIRAKPARARGIGEKLRLLPKVRRRWLILIYPGFPVATAWVYRNLSLKLTKVGVN
                     TSIATPLESLDKLDKLLINDLEQVTVRRYPEIDHAKSLLFQAGAAGVLMSGSGSSVFG
                     VFKSKRLAAQAFRQMRHQEGVQAFLVHVLN"
BASE COUNT         4587 a         5397 c         5121 g         3926 t
ORIGIN      
        1 tcgcgctgta tattttgatg cacgaattgc cgctcgaggc gacgccattc ggtccggaaa
       61 acaaagtcgt catgatgacc ggcccgctca ccggcaatgg cttcacaccg ggcggcacga
      121 aaatgaccgc cgtctatctg agcccgctca ccaacacgac cctcggccgc ggcgctacca
      181 gcggttattg ggcgacctat ctcaaagccg ccggctatga cggcgtcatc gttgaaggcg
      241 tgtcggcgaa gccggtctat ctgtacatca aagacggcaa agccgaattg cgcgatggcg
      301 cgaaggtttg gggcttggga acccgcgaca ccgaagatcg cctgcgcgag atcgtcggca
      361 accgcgacgc cagagtcctc tgtatcggcc ccgccggtga gaacatgaac cgcggcgcca
      421 tgctcgccaa cgactacaat catttcgccg cccatagcgg cggtgccgtg ctcggttcca
      481 aaaaactcaa agccatcgtt gtctcaggaa cagtaaggcc aagttatagg gacaaggtaa
      541 aactcgccga cgccggcttg cgctggcgca atgttctgca gcaacacgac gtcaagaaaa
      601 agaaaaccaa gtacggccat ggcgatgcct ggggcgcact gaataatttg aactggcgca
      661 gcaccgacat tcaaccggcg catatcgccg gtttcgccga gaacgatgtc gtgctccgtc
      721 cgtgctttca gtgcgcccgc atgtgtccct gggacgccaa gttgggtgaa ggcgagttca
      781 aaggaacggt cgttcacttc aacgccggcg gcgaatggct cgatactttt ttcaatctcg
      841 gcatcaaagg caatgccgtg ctttacctcg ccgagaagat caacaatcta ggcatcgagt
      901 gcagccactt ttcctgcggc gccggcgtcg cgttcgaagc ttgggagaaa ggcttgctcg
      961 gaccggatcg caccaacggc atcaaactcg aatggggcaa cgtcgatgca gtcagtaaat
     1021 tgctcgacat ggccgcccac cgcgaaggct ggctcggcaa tcttctggct gaagggccca
     1081 agcaactggc cgaagctctg ggtggagacg ctccgaactg ggcagtccac accaaaggcg
     1141 gcacgccggc catgcacgag tggcgcccgc tcctcggtca aatgttgcgt gagctggtcg
     1201 ctagcggtgg catgaagccg caaggcggag gctcgaagaa tccgccgccg gatctgcgcg
     1261 acaacgaaca ttggggaccg ttggaaatgg ataacccgga aggttgggtg tggtcgcatg
     1321 tcttgtccga gcaataccgc caattcaccg gcatattcgg cggctgctgg ttcgcccagg
     1381 aaagtcacaa aaaagacggt ttgaattcca tcgtcgacgc attcaacgcc atcaccggct
     1441 ggaatttttc catggaggaa gcgttgcaag ccggccaccg cagcatgatc ctgcaaagcc
     1501 tgttcggcac acagcgcggc tgggtcgccg acttcgactg gcaagacgtc ggtccgaggt
     1561 ttttagaacc gatcccggac ggcaagtaca aaggtttcac catcgccaag tggctgcctg
     1621 acatgatctg ggattactac aaacaatccg gacgccacga gcgcaccggc aggcctttta
     1681 aagacacgct ggagaagctt ggcttagaag aattccaaca gtggagccaa ctcgactgat
     1741 tgcaatcgat tacccagcga atggtggcaa ctcttccaac aaccggctcg cgccgattgt
     1801 tggattctta atcttcaaaa gcttctgcac gtaccacacc cgcgcagcga ctggatgaac
     1861 caccggtacg ccgagatcgt tctcggcggt gacgatgtcc ttgatccccc aggcgccgga
     1921 accgagcaga taaatgccct gcgcctttgg atgtttgtta aatgacgcct tcaaatgccg
     1981 gtagatggtc tcgttagaaa tttcgtcggc ctcgctgggc gatgtgtcca tgccttccat
     2041 gcccaacact tcgaatccgc agtcggtgaa gtatcgggta aagacatcgt tcatcttctg
     2101 atcgcgaaaa taagtgcagc cgacaaactt tttgatccct aaagcacgca gcgcttgggc
     2161 ctgcgacatt cctgatgtga aaatcggaac tttatacttg gcttcccaag cgccgacgat
     2221 ctcctgctct gcttgatgtc cgcgcaccat gaacggcggc ccgccttcgg gatgcatcag
     2281 atcgacgccg atttcagcca gctcggcaac tttggcatga taggtttcca tggccccaag
     2341 gtagccgcgc tcagaatgtt ccttgatgcc ggcatacatc ggaatgcagc caacgccttc
     2401 cggcaagagc cggataaatt ccaccagcga tcccgagcca taggtcggtt tcactacgcc
     2461 gacaatgcca cgccacgttc cagaagccat gtgaatctcc ttcccgatcc gcaacactcg
     2521 attcatcgca tgctagtcgg gacagccgcc aaaaaacaag agctcgtaac gcaggtttac
     2581 cttccgttct ccgttatcaa ttctacattg attgctactc tgttgacgtt cataaaaact
     2641 aagtgataaa cacgttgaac gaaagtgagt gaggagcagc atggcgatca aaccgacaga
     2701 agttttaacc tgcgaatacg gcaatctggt tcccactggg cggctacttg acggcaagcc
     2761 gcgcagcatt ttatttgacg cgaccaagtg catcggctgc cgccactgcg tgcaggcatg
     2821 caaagattgg aacgatcacg accgcacgag tctttaccaa ctctccagca ccaactggat
     2881 caccatggag ccgccggtgc tcgaaggttt atcaccgata tgggcgagga acagctgcat
     2941 gcactgcgac ttcgccgcct gcgccgccgt ttgcccggtc gaggcgatca ccaaatacga
     3001 agaaggcgcc gtggtcatcg ataaggatgt ctgcatcggc tgcgagtact gcattcacgc
     3061 ctgcccgtgg gaagtcatca ctaaaaacga gatcaccggc aaggccagca aatgcaccat
     3121 gtgcagcgac cgcattagcg aagacaagca gcccttctgc gtccaggcct gtccggttga
     3181 tgccctcgac ttcggtttca ctgaggaaat cgccgacaag gcaaaaaaac aagcaaatgc
     3241 aattggcggt tatacctatg gcgataaaga agccggcggc ggcagcgtcg tctacgtctt
     3301 gaaagagggc aaagacaaat acggtctacg cgacatcgcc gccggcagat ttcccaagca
     3361 ccgtatcccc ttgggtttga tgctcaaaga tctgttcacg ccgcgctgcg gcgttgccgg
     3421 caaaatgcgc gccctatggc ttgccatcac ccatccaaaa agactgattt accggtattt
     3481 ctcgtgacgg taacaccgcg ccaagtatct cgatcactcc tccttgcgaa acctaacatt
     3541 ccccctcgac ggggaaaggc caggatgggg gtgctgcgca gtcccttgaa attgacgggc
     3601 attcccactt tcattttctc ccgtcacggg agaggcaatc ggatcacgac atcgcgaact
     3661 gagcgagcag tggtggctgc gatcctttcc gttatagtag tggggattgg aacacacgcc
     3721 caagccgaaa aaatccgcac agcgattccc caagccaatc tcaactatct ttcaatctac
     3781 gtcgccgatg gccgcggatt tttcaaagat gaaggactag acaacgaaac cgtcgtcatc
     3841 tccgggccgc tcgccacggc tgctctgctc agcggcgatg tggactatag cgggggtggc
     3901 ggcagcggca tgcgcgcggc cattaagggc gcaccgataa agggcatcta tttccagtcg
     3961 gaaaaggtaa ctttttactt ggtcgcagag ccgagcataa aatcggcggc ggacctaaag
     4021 ggcaagcgcg tcgcagtggg aagctccggc gacacgcagg accggatgat taccatgttc
     4081 atcgagcgcg gcggcgtatc gtcgcgtgag atagttcgaa tctccgtcgg gtcggacacg
     4141 gcgacgcgta ttttggcaat caaaagcggg aacgcgcacg ccaccacggt ggatccgggc
     4201 ggtttggttt tcgctcagaa agaagggctt gtctcattgg gatttctcgg tgatttgttt
     4261 ccgatgccgt ttcaaggatt cgtcgctacg gatagaaaaa ttcgtgacaa cccgtcgcag
     4321 gtcaagcgct ggcttagggc agcgattcgc ggcctgatgt ttgtccgcga tcggccggaa
     4381 gaagccgtgg acctcggcat gaaaaaactc cagctcggcc gcgccacgaa agcgatgatc
     4441 gtcgagggaa caaagaacta tgtgcgtgcc ctgccccaag gggtgcccgg catgccgtcg
     4501 gcagagggca taaagaactt tcttgaatac gacattaaga ttcctctcgg cttgaaagag
     4561 gatattcctc ctgagcggct gctgcatttg cagctggtca aggaagtcaa ggaggaagtg
     4621 gaaatggcac aaaggagaac gaaataaatg gcaaccgaac aacaattgcg cgaacagctg
     4681 gcaacttgca cgcgaatctt cgccatgcaa gggctgattg gcttattcgg ccacgtcagc
     4741 gtgtttcagc cggaaagcaa aacggtgctc gtcacccccg gttcgggttc ggacaaggcg
     4801 cactttagtg cgtcggacat gatccccatc gatcttagcg gtaaaccgat caacggcacg
     4861 ataccgccgc cagtggagtg gccgatacat accgcattac acgcggcgcg ttccgatgct
     4921 ttggccgtcg ctcacctgca cacgccgtac gcaaccctgt tcgccatcgc caagcgcgag
     4981 tttcgtccgg tcacgctgca aggcacggtg ttcggagaag gcgtgccgct ttacaaagaa
     5041 gctcatctgg tcaccacgcc gcaacgcggc gaggccatgc tgaagaccat cgggaacaat
     5101 cgtgccctgc tcatgcgcgg ccacggcatc gtcgtcgtcg gcaggaccct ggcggaagtc
     5161 ttattcgcgt cgttgatctt ggaagacgac gccaagaaaa ccatgcaagc ggccccgctt
     5221 ggcccggtgg aacatttcag cgcggaggaa tgcaagagtt ttaatgccga aatcgatttc
     5281 gagcgccgcg ccaaccgggc gtgggagttt ttttcaagaa tagaatcaca gtgggaccat
     5341 caaggcggca cgggtaaaat agaactgttc ccgtaggacg ggaacagtgt tccaaatgtt
     5401 ccagagttcc agtggcgatc ggatttcgaa cctttggaac gattggaact tctggaacca
     5461 ctagaactaa ggaggcttta tgagcgaagg cgtacgttta tacgcaggca cccaacatgg
     5521 attgatcact tggaagtcaa ctaatgccgg ctggcaggaa gtggcgcgcc atttcgaaga
     5581 cggcatcatc gattcgatcc acggctgcaa acaggcaccg gagaaggtct tcgtcggtgt
     5641 gacccatgac gggctgtatc ggacaagcga tggtggcaac agttgggcaa aggtattcga
     5701 aggcgacatc cgctcggtct ccgtcgaccc gagcaacgat agcgttgtct acaccggcat
     5761 agaaccggtg ggactgtttc gcagtgaaga cggcggcgac agttggcaag aagtcaccgc
     5821 tttgaaaaaa ctgccgcaat cggtgcgcaa gaactggtgg tacccgcagg cgccgcacca
     5881 tggacacgtg cgcaacatcc atatccatcc ggacaatccg caaacgattt acttatgcct
     5941 ggaacatggc ggcatcgtgc gtaccttcga ccgcggcgcg acttgggaag atgtcagtaa
     6001 gggcatcgac tatctcgaca tccacgtgat cgccaatttc cctaagcgtt acgatagata
     6061 ctacgtcgcc agtgcgcgtg gttttttcac cagcgacaca cctgaaaccg gttggacccg
     6121 cgccgagaac gggatgaccc gcgactattt ccacgatttc ctatttctca atcccgaaag
     6181 cgaaagcgag ccggcgactc tcctcatcgg cgcggcggat gcatcaccag gattctggcg
     6241 ccgagaaaat cgcggggcgc gagcggcgat tttcaaaagc accgacggcg ctcagtcatg
     6301 gagccgagtc accaacggcc tcgctgaaga gctcgactcg atggtctggg cgctggtaca
     6361 tcatccgacc gatcacaagt cagtttttgc cggattcggc aacgtcgccc gcggccacgc
     6421 ttcgggtgcc ggtggtgccg gtgatctgat gattagtcgt gacaagggcg atagctggga
     6481 gagactcgac atcgatttac ccgcggatcg cgtcttgtgg gctgcggcgg aataggtaaa
     6541 cgctttgcgt tcgtgttcca actgatcaac cagttcaaag ggtatcgagt cttcgagacg
     6601 gttttaacgg cttgaacgat ttgaaccatt tgaaacgcgc ctaaattcat cgaggcagag
     6661 aaatggccaa ttccgactac accaactttt ttccaacgac acgtaatacc cgtttgcccg
     6721 gcggctacat gggcaaaatt ctgcgcgtcg atatgacaac aggcaaactc accgatgtga
     6781 atcttcccga agaagcgctg ctacgaaaat attggggcgg ccaactcttc gccgaatatg
     6841 tcttactcaa tgaactgccc ctggacatcg atccttacga ttcacgcaat gtcatcgtcg
     6901 gcatgacggg tccgatcacc ggcaccggct ttactcccgg cgggacaaaa atgtgttttg
     6961 tctatctaag cccggcgacc aaatacactc tcggccgcgg cgctaccagc ggctacttcg
     7021 gcacgtcgct aaagaacgcc gggtacgatg gcatcatcgt caccggtgcg gcgctgacgc
     7081 cgaagtatct ctacattggc gaagacaaag tcgagctgcg cgacgccagc catgtatggg
     7141 gcaaagggat tcgcgagaca gaggacacat tgcgcagaga ctccggccac caggatgctc
     7201 gtatcggcgc catcggaccg gccggcgaga atctcgtgcg cgcggcgatg ctggtcaatg
     7261 attacaatca tactgcggcg cacggcctcg gcgcggtaat gggctcgaaa aaactcaaag
     7321 ccgttgtcgc ctggggcaca aagcggccgc gcttgcacaa taagaacaat ttaatcgacg
     7381 ccggcgtgcg ctgggccaaa gcgatcgcat cacgcaaaac caccgtgaag aagcgactaa
     7441 ccagcgtcgg tcacggcgag gactggggcg cgatcaccaa gttgaattgg cgtagcacaa
     7501 taataacaga cgaagccagg ggcctcgatc aaaatcgcgt tacactgcgt ccctgctttc
     7561 aatgcccgcg catgtgtcct tgggacgtcg agattggcga gggccgcttc aaagggacca
     7621 tcggtcactt caacgcgggc agcgagtgga tggatacgtt ttacaatctc ggcttcaaag
     7681 gcaatgacgt gctctattta tccgagcgca taaacgatct cggcatcgag tgcagccact
     7741 atgcctgcgg cgccggactt gcgttcgagg cttgggagaa aggcgtactc ggcccggaca
     7801 agacggacgg tttgaatttg gaatggggca accttgaagc ggtcaagact ttgctcgaac
     7861 gctgcgcccg ccgcgaaacc tggctcggca atatcctcgc cgacgggccc aaagagttgg
     7921 cagaagaatt gggcggcgaa gcgaaaaatt gggtggtgca caccaaaggc ggcacgccgg
     7981 cgcagcacga gtggcgcccg atgttgagcc agatgctgcg cgagttggtt gccagtggcg
     8041 gcatgaaacc gcagggcgcc ggcggcaagg aaccacccga cgatcttaag tatcgcgaga
     8101 aatggggccc tctcgatccc aatcaaccgg acggatggac cagctcgcac ctgatcacgg
     8161 agaaggttcg ccaggcctgc ggcatcatgg gcggttgctg gttcgccctg aacgacaaac
     8221 cgcgcgacgg tgtgcagagc atgctcgacg cgcttaccgc cacgacgggt tgggacgtga
     8281 ccttggacga actcatcgac gccggtcacc gggcgattat tttgcagagc gtcttcggca
     8341 cccagcgcgg ctggatcgcc gaacatgact ggcaagatgt cggcccgaga ttcttagagc
     8401 cgatccccga cggcaagtac caaggcttca ccatcgcgaa atggctgccc ggcttggttt
     8461 atgaatatta caaaatctcc ggccgccacg aacacaccgg ccggcccttc atggacacac
     8521 tgcaaaaatt aggcatcgag gaatttaaag agtggagcca gctggattga tccgatcgac
     8581 gaggagataa aaatgcgagt gcgtaaattt ctaatactgt ccctggtaac acttttggtt
     8641 gcgacctact tcgaaccgcg cgcctatgcg caagcactga aaaaaatccg catggccagc
     8701 tcatcgacca atgtctcatt cctggcactc tacacggctt tgcaccgcgg ctttttcaaa
     8761 gacgaaggca tcgatctgga aatcatcttc atgccggcca acttggcgag caccgcggtg
     8821 ctaaacggcg atgtcgactt taacggcgcg gtgaccggca ccatcggcgc cgcggtgcag
     8881 ggccggccga tgaaggtatt gctgttcacc gtgtcaaagc cgctattgtt cttgatggga
     8941 cagaaaaaca ttaaggactt gaagcaactc aaaggcaaaa aaatcgccgg cagttcgcct
     9001 ggcggctcgg cgaccttgct cgccaaccaa gccctcaaac aaatcggcct ggagcccggt
     9061 aaagacgtct ccgtcctgca gatgtcaggc aacgccgcga gccggctggc ggtcttggaa
     9121 tcaggcgtcg tcgaagcatc actgatttcc gtgccggaaa atattatcgc tcaggaaaag
     9181 ggctttaacg aactgctgtt tctcggcgac gtgatcgagt ttccccagaa tggattcggc
     9241 acatcggaca aacgtattcg tgaaaacccc gacgaagtgt accgcatggt gcgcgccacg
     9301 ttgcgcggct tgcaattcat ttgggataag aacaacagcg aagcggtgac caatatttta
     9361 atgaaacaat ggaaggtgag cgaccgcaaa atggccgccg aaatgtcgcg acaagtcggc
     9421 agggtgctga ccaaagacgc gtatgtcaaa ccggaatcgg tgcaagtgct catcgatctc
     9481 gcccgcgaga gcgcgaaagt aagcaaatcc gtggctgtcg ccgaagtcgt agactacagt
     9541 tttttggaca aagcgcgcaa ggaattgggg atgataaaat agctcgtacg ggcgccgcat
     9601 gctgcgtccg actgcaccat caaagcagcg gatcgatgat gaccttgatg gaatcttgcc
     9661 cgcttgccgt tagctgaaag ccccgtccgg tctctgccaa gggcaatcgg tgcgtcacca
     9721 tgtcggcgac acgcaccttg tggtcgcgaa tcaaagccag cgactctttc aaatcgtcgc
     9781 cgctgccggc gtaagaagtt accacgttga tttcatcgcg ccagaaatcg aacagcggaa
     9841 tcggcacatc gacgcccgcc gccgtcggcg caaacaatag cagcgtgccg ccgcgatcta
     9901 ccgacttcgt cgcttgcgta atcgccgcca tggcgctggt gcaaacaatc accagatcgg
     9961 cgagacggcc gccatttaaa tcctttagcc gatccggcac attttgcgcg ccgttaattg
    10021 tcgcatcggc gccaaattgt ctcgccgcat tcaaacgata ttcgctgata tcggtggcga
    10081 tgacccggtc ggcgccgcgc gctttggcga gttgaatgtg cagcaagccg gaaataccgc
    10141 tgccgattac caacaccgtc tgcccagtcg caagcttggc gaaccgctgc gcccgcacga
    10201 cacaagccag cggctcaatg aacgaacctt cgtcaaacgt catcgcgtct ggaatccgca
    10261 acgtgccgag ctcgacgttg attttcggca cgcggatgta ttcggcaaaa ccgccgggct
    10321 cgaaatgggt cgtgcgcagc agatcgcaca ccgactgatg cccggtttta cagtaacggc
    10381 atgtgccgca agacacgtgg tgagtggaga acactcggtc gccgactttg taaccggcaa
    10441 ctccagcgcc gatctcgacg acctcggcag tgatctcatg ccccaacacc aacggcgctt
    10501 ttttgatacg gtaccactcc atcaagtcgc tgccgcaaac cccgctagcc cgtgtccgca
    10561 gcagcaactc gcccggacca atagcaggaa ccgggagctc ctccaatcga acatcccggt
    10621 tgttgtagta cattgcgacg cgcatgttaa ttgttcgcgc aacaagctgg agcaattttg
    10681 ctatcagctg gctttgcgtg cctgggaatt cttctcgctc tcgtagatct ttaaggcctc
    10741 ttttacagaa atattctcat ggacgattgc acgcacggct ttgatcattc ccacgggaga
    10801 atcggactgg aagatattcc ggcccatgtc gacgccgacg gcgccgcctt tgacggcgtt
    10861 gtaggtgagc tctagcgctt ccagttccgg cgtcttcttg ccgccggcga cgatgaccgg
    10921 gaccggacag ctgccgatca gctcttcgaa attgtcgcag tggtaggtct tcacgatatg
    10981 cgcgcccatc tcggcggcaa tgcggcacga caacccgaga tagcgcacgt cgcgcgccat
    11041 gtctttaccc accgccgtga ctgccagcac gggaatgccg tacttctcgc cttgatcaac
    11101 caacttggcc aaactgacca aagtctcttt ctcgaattcc gacccgacga agatcgacag
    11161 cgcaattgcc gagacgttga gccgaatgca ttcctcgatg gacactgtaa tctcttcgtt
    11221 cgacaactct ttgagaatgc tcgtgccgcc cgagactcgc aagacgattg gcttgtcggc
    11281 gctggcgtcc acggacgtgc gcaacacccc gcgggtcagc atgatagagt ccgcgtacgg
    11341 catcaacggt tcgatggtct tgcgcggctc ttccagaccg ctggtcggtc cgagaaaata
    11401 gccgtgatca acggcgagca ttaccgcccg tccgtccgct ttgataattc gcgctaagcg
    11461 attcgccatt ccccaatgca tcgacatagt agcctcctca ataagagctt aatgggccaa
    11521 aaactatcat aaatcgattt tgaatgtgaa tcccgccgtg caatttgaca gcgtcggcct
    11581 agaactatag cttggcatca ccaataactg aggaggaatt atggcgattg agatcgtgga
    11641 catggttaaa aaggccggcg aaggcaaacg ccaagtgatc tttaacaccc atcgttttca
    11701 cgcctgggtg catgtttacc ccaagcctgg cgacgccgac gacatgcatt gccacaacgc
    11761 cgaccagacg ttttatgtca ttgacggtga atgcaccatg cattttcccg atggcggcaa
    11821 agcggtcatg acgccggggc aggtggcgac catcacaggc ggatcctttt accagttgga
    11881 gaataccggt accggcccta tggtcctgat gggaaatcgc tccggacctt cggaagcgat
    11941 ccaacatatc aattacgagt tgcgcaagga catcaaaacg ttatcgaaag aggagatgga
    12001 caaaatccgc catcccggca acgtgcgtgt cgccggataa agaaagacct caactcgttt
    12061 gattgatcgc tttggcatgg gcgtgcgcgc caatgagtgg ccaaagatag cgcacaccga
    12121 ggccgaatag gatcatcgca ccgccccaaa ctgtcgctgt tgagggaaac tcgccggcga
    12181 tcaaagcgac ccaaagcgga ttaagtaccg gctcgaccaa ggcgatcagc gacgcctctt
    12241 gcagggacac cgagcgcagt cccttggaaa acagaaaata cggcgcgccc aactgcaccg
    12301 cccccatgat taccaggatg acgatctgag tggtcgtcaa ggtcaaatgg gattgcacaa
    12361 tcccgaaaat caaaaacgca cagacgagat tgttgaccca tgttaaataa accggagcga
    12421 cattgttaat gcgttgcaaa ttgatcatat aaccggcgaa cagcagaccg ctcagaagcg
    12481 cgagcgcgac gccggccatc tccggctcgc cggcgctgtc gaaggaaata atcccgatac
    12541 cgatcatcgc cacgccgagt gcaaagatat tcggcctggc gatttgttcc cgcatgacca
    12601 accatgagaa caagaatacg aatatcggcg cggtgtactg aagcacgatg gcgttggcgg
    12661 cactggtgag cttgttggcg gaaacgaaag cgctgatggc cgccgtgtaa ctcaggaccg
    12721 agaagataat cctgcgatta tagcgaaagt cataacgctt cagaaacggc gtaaatacta
    12781 tcgccgcgaa aagactgcgg tagaacacga tcgccagcgg gtgaatatcc aaaaacttga
    12841 taaagacacc gccgagactc cagagaattg cggcagcgag gatgaacagg cgaccgcgct
    12901 caagctgcaa tttagaagcg aacttggatg ctacgtatgg catggcgcgg gagttcgttg
    12961 tgagcagcgc gtggacgtcg accgcaattc gtttagaact aacaatactt gtctacaaat
    13021 aaaaatgggg cggaacgcat aggcccaccc catcggattc gaatggttcg attaaactat
    13081 cgaatcagtt aatctttgct atcggatacg acggacactt tgagcatcac cgtgagatcg
    13141 gccgacaaac gcaccggaac ttcatattcg ccggcgactt tgatcgtttc gttcaattgg
    13201 attttgcgcc ggtcgacgac aaggcctttg gccttaagag ctttgtcgat ttgcatattg
    13261 gtaactgagc cgaagagttt gccttcttcg ccaaccttca tcgggatcac cagcgatact
    13321 ccggacaatt catcgcccac cgcctgagct tgacgcagga ctttttgttt ctggtcggaa
    13381 ataatttttt tggagtgatc gaagctcttg agattcttct tgttcgcctc caagactaat
    13441 ccgcgcggca gcaaaaaatt tcgcgcgtaa ccatcgcgca cgcgtacgac ttcgccaatc
    13501 ttgccgacgt tggcgatatc ttctttgagt ataacttcca tgattcctcc gctaggacgc
    13561 tcggctcgga gtgagattgt ctttacccaa gcggcggaaa tcgccccaga gatcgaacag
    13621 ccccagacca acaaccatga gcgtaaaaat ttgctgaaac acgatcaaaa tataggtaat
    13681 gccgcgcagg aatcgcggca cgttgttctt gtgaaaaaaa aacgcgatca cggccaaacc
    13741 ttgagcgaaa taaaaagcac cgacgatcaa taacacgttc atggcgatca cacgcacaag
    13801 atcaggaacc gggaaaaaca agctaaaccc gcacgcgatc aaaccccaaa ccagcgcttc
    13861 cggacctttc caatcgcgaa agttttcaat ggcaaaccat ctggcgcggt gctccggaaa
    13921 acgccgacgc agcgaaagaa gattgatcaa ggcgatgaac gcaaaggtca gaaacattaa
    13981 tcctggtaaa agttgcaaag tcatgtcgat caactgcggc attctttcct tgagcagttc
    14041 caaactctct ttggagaaac ctagttgctc ctgcatccgt gtagcggcgg caaactgttg
    14101 ggttaatccg ccgcggaaat cagccgcgag cgcggaccaa gagccaaaaa aataacgggc
    14161 gacgccgagc gaccaaacaa gcattgccga ggccgcgccg acaacgacat actcaaccat
    14221 acgcaaccgt ccaacgagcg caaacaagac agccgcgaga atggcaaata ttccgtacaa
    14281 cagactcagt tcttcggcag caaagagagc tagcagcaat gtcgcgacga gcacgacagc
    14341 tagaccgtaa ccgactccgt atttaaaccc gaacaacagc acgggctgaa tgacgaaggg
    14401 cagcagcagt gcaccgatgg gcggcacact gataccgctg atgaacagaa atacactcaa
    14461 ggctaaagcc aatacaaacc gggtgatgac ttctagccgc tgcatagata tggccgaaga
    14521 actgatttaa ttattcgcgg tggtgaacgg cagcagagcg atgcttcggg cgcgcttgac
    14581 cgccgtcgtc agcagccgtt gatggcgcgc gcagttgccg gtaatccggc tcggcgtcat
    14641 cttgccgcgt tcggtcacga acgaagatat cgtgcgcaag tctttgtaat cgattcgtaa
    14701 gctcttgtcg gcgcaaaaac gacaaacctt gcgccgaaac atcggccgtc ggcgctgacc
    14761 gcctttatca tcgtcggacc gttcgcggct aggcctcggg ctgcgttcat actgtgccat
    14821 aatttacgct acctcttgtt tcgtctgctc tttcctggcc gtggataaac cagcgactgg
    14881 agatcaaatc attctgaatc tggtacttag gtcggaactc gcgtcataga taaaaatcgc
    14941 agtggaccga tcgcgccatt aactatctag gccgaggggt cgcggtcgcc gccacgcgat
    15001 ctattatcgt cgtagttccg tcgatcgtcg taatttcgcc gcggcggctc atcgctatac
    15061 gcccgcgggg cagatggact ggtcattgga ctgtcctcct ctgcgcgcgt cgtcaggtaa
    15121 cgcagaatgc cgtcggtcag cttaagattt cgctcaagct ccgccacggt ttttcctgtg
    15181 gccttgtagc gcatcagcgt atagaaacct ttgccttgtt tttcgatacg ataagcgaga
    15241 tcgcgtagac cccactcttc gacctgcacg atgctgccct cttggccttc gacgaccttg
    15301 ttgaatcgct cgatgaattc tttgtttctc gacccttttt ccggatgcac cacaaaaaga
    15361 gtttcgtata atgtaaccgt gtgattatcc tccgcaagaa aattgtctat ctaacatacg
    15421 gacttggcgt ttacaatgta cagcggccgt cagtccaccc gattataaat agccattgcc
    15481 gcctcggctc ccttatcaag caaacagcag agcgcctcgg tggcgcggtc catgagctta
    15541 gttaggtcac caagctcgga ttcattgaac ggtgccaata catactcgat aacctcgaga
    15601 tcttcgggag gacgaccgat gccgagacgc agacgacaaa acgccgcgtt gccgagatgt
    15661 tcaatgatcg acatcacacc gcgatggccg ccggcgccgc cgttagtgcg aatcctaatt
    15721 cgcccgaacg gtaaatccaa gtcatcatgc acgacgatca aatctgccgg cgacgcacct
    15781 tgctcttgaa gcaatgccgc caccgccacg ccgctgcgat tcatgaaagt ctgcggcttg
    15841 gccaacagca gcgtagtttc gtttactcgt ccttttccag ccaaggcgtc gcactggtag
    15901 tggtctaaaa cgattccttg ctgccgcgcc acccggtcga taaacaggaa tccgatatta
    15961 tgccgcgtgg cgtcgtactt gttcccagga ttgcccagtc caacaacgat tttcaccaag
    16021 ccaatcccgc tgaaagagcg aaacaatcgg cctaagaggc gctgtccttt ttagcatccg
    16081 gcgcagcaac tggcgcagct tcggccgcgg gtgcagcggt ggtcggagcc tcctcgacag
    16141 tgggcgggac aacggtcacc agcgccaagt tgtcttccga aagggacaca accccctccg
    16201 gcatctgcaa atcttcgata tgaaccgaat cgccgatatc cagcgcgctt acgtcgacgt
    16261 caaaaaattc cgggatgtcg aggggcaaac attcgacctc gatctcgcgc acaatgggct
    16321 gcaagattcc gccacgcgcg gtccccaccg ctttgccgac aaagtggagc ggtacatgca
    16381 cctgaatttt cgccgtcaga tcgacctcgt agaagtcagc atgaatgaca tcgccggtaa
    16441 tgggatgaaa ttgcatatcc ttaaccagcg caactttttc cgccagggtg gccgcgccgg
    16501 atttcaaccg taccaggtgg gaaccttgca agcctgcgac gcgccgagaa aattctttct
    16561 tgtcgagcgc caggcctacc gcggccattt tcgggccata taatattgct gggatcttac
    16621 cgctgcggag taaccgcttg gcatcacgtt tacgctgctt atcgcgcgct tcaacctgta
    16681 tttccagagt ctccacggaa ccacctcctc tgactaaacg aatagcgaac tgatcgattc
    16741 ttcttcatga gttcggcgaa tcgcctcacc gatcaaatgg gcgaccgata atattttgat
    16801 tttgctacat ccctgggcgt gcggtccgag aggaatcgtg ttcgtgacta tcagttgctc
    16861 gagcggggat tcttcgatgc gtttgatagc cggcccggac aacaccgcat gggagcagca
    16921 gccaataatg tttttagcgc cttctttttt gagcgccacg gcagccatgg tcaacgttcc
    16981 ggcagtatcg accatatcgt cgaccaaaat ggcaatgcga tcctgcacct caccgataat
    17041 attcatctcg gcgaccacgt tgggaccgac ccgacgcttg tcgattatcg ccaaggacgc
    17101 atccaaacgc gtcgcaattg cgcgtgcgcg ctcgacacca cccgcgtcag gcgagacgat
    17161 ggtcacctcg ttatggttga gctgcttacg taaataatcg agcagcaccg gcgtcgcgta
    17221 gacgttgtcg acgggaatat caaaaaaacc ttgaatctgc cctgagtgaa gatccatggt
    17281 cagcatgcgc gacgccccag cacgggtgat caagtcggcg accaacttgg cactgatcgg
    17341 cacccgcggg acgaccttgc ggtcttggcg cgcgtagccg taataaggca taaccgcggt
    17401 gatgcgttgg gcggacgccc gtttgaacgc atcgagcatc aataacagtt ccattaaatt
    17461 attattgcct ggagcggagg tagactgcac gacaaagaca tcggcaccac ggacattttc
    17521 tttgatttca accttacttt cgccatcact aaacgtatcg acggttgcct tgcccagcgg
    17581 cactttaaga tattgactga tctcagcagc cagcggcgga ttcgagtttc ccgcgaatat
    17641 tttcaagtcg gctgtgcggc ccatatatcg actaccccgc agtgcgaatt aacttatgac
    17701 gtgacatcga tcgtcaaaaa acacgagcgc cgtccaactg tgaacggcgc cattcaacct
    17761 tactgaatct atttcgaagg ccaacccagt gatcttcgca tagaaattgg ctgggcggga
    17821 aggattcgaa ccttcgatac cggctccaaa ggccggggcc ttaccgcttg gcgaccgccc
    17881 atccctgaac cccgccggga agtttatcca tccgcgcaga acattcgatc agtcgtcatg
    17941 aaccgatgtg ggtgtcacac catcggctag gattttttga tattctgcga tgattttttc
    18001 ctcgagcatc cgacgagtct cgttattaat tggatgcgcg atgtccttat aggttccgtc
    18061 cttgcgcttt ttgctcggca tcgagacgaa caaaccagta gcaccatgta tgatttttaa
    18121 gtcccggatc acaaaacagt gatcgagagt gatagttaca taagccctga gcttatcttc
    18181 ctcaacagga aagaccctaa cctcggtaac ttgcataggg acccccttct gggcgagcca
    18241 gatgttgcgc ggtagagttc tttaatctgg gcttttgact aacctcaatt caaaacatgg
    18301 acaagaaacg cttgtacccc ctcctgatgc cgcatctggc ggaacgcttg tgccgccaat
    18361 cgcttcgatt taaaaacgcc aaacaccgac gagccactcc ccgacattaa aacacccgcg
    18421 gcaccagctt gaaatagcaa agacttcgcg tgatctattt caggatatct cctcacggtg
    18481 acctgctcta aatcgttgat gagcagtttg tcgagcttat ccaggctctc cagcggcgtc
    18541 gcaatgctag tatttacgcc aacttttgtc aactttagcg agagattgcg gtatacccag
    18601 gcagtcgcga ccgggaatcc cgggtagatc aaaataagcc agcggcgccg caccttcggc
    18661 agcagtcgaa gtttttcacc gatgccacgc gcacgcgccg gcttggctcg aataaagaac
    18721 ggcacgtcag cacccagttg cagggccaaa cgctcaagtt ggcgcaccga caggcgcagc
    18781 ttccaaattc ggtttagacc gatcaatacc gctgcggcat cggtgctacc accgcccaat
    18841 ccagcaccga ccggaatctt tttttgaata tggatcacta ccggctgacg aatctttgac
    18901 ttacgcatca acaattccgc agcacgataa acgatattgt ctcgacctga gggcacttcc
    18961 ggatgattgc aacgaatttt gatcagcagg ggaatttcgg attgctgtga tgtgattcgg
    19021 cgaatctgtc g
//
LOCUS       DATLCH010000342         3626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3617844, whole genome shotgun sequence.
ACCESSION   DATLCH010000342 DATLCH010000000
VERSION     DATLCH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617844"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1160)
                     /locus_tag="VMZ02_10145"
     CDS             complement(147..1160)
                     /locus_tag="VMZ02_10145"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HUR72339.1"
                     /translation="MSTHNVSTPTHERCVTSYAARTQYKPGTVHPPHIKVKDAIDIHC
                     HAHEGQQDPFDLARRASLAEMKGLLFKSIPGDDPVKATQAVQDDVNRWAEKQEGVKPI
                     TCWSAYVCGRQMAPVTAKIVSDMIDHGVTAIWLPVFTHVITMSTVGTYGRFVRGRENA
                     GWVGPIPWDEALKTGHYDLDDNGKLKPEIRDIVHLCADRNVALFFGHATHAEIYKLAE
                     EVQKVGLKRAVIDHPFSPFLNVSPDMMKEICGTGVLFNFTWDELSPLLGVDPQIMYNT
                     IREVGVEHFTLSSDAGEPLFPDSVEAMRLVRGYMEAFGLNQEELYTVCTRNPAKIVGL
                     ELN"
     gene            1347..2354
                     /locus_tag="VMZ02_10150"
     CDS             1347..2354
                     /locus_tag="VMZ02_10150"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72340.1"
                     /translation="MLQHFARLAHCACPMVLLVSLLPWEILAAADRVNMIYTARVMSQ
                     AYPWIAQEAGLFKKYDLDVPMVFVTPGAPSVAAILSGDSEVAVIGAASITRPWVQGNK
                     DPVFVGGIKSLLTHSLIAKSDIARPEQLKGKRIGVSRIGSNPHYFAVQALRHYNIDPR
                     EVSFIQAGGAPETLAALVAQGIDAAVLTVPTDAQALKLGYHYVVYGPDLKIAYAATTF
                     ITRRTIAAKRGPVIGRFMRAMAEAARIMHVDREFTYKVLEKYLRVDDRKLLEASYNVE
                     IKALEPRLAMKPEGFQSTLDEIAPTEPRAKTVKPQEMMETRYLDEMEKSGFFEQIWGR
                     K"
     gene            complement(2455..2928)
                     /locus_tag="VMZ02_10155"
     CDS             complement(2455..2928)
                     /locus_tag="VMZ02_10155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR72341.1"
                     /translation="MKHSESTLRLARAADAKAIANMSRDLIESGLDWRWTEQRVAANI
                     DDPAINVLVARLDNRLAGFGIMKYGERVAHLNLFAVAPDCRRAGIGRQLLHWLERCAS
                     VAGNVAISLEVRAGNTGAQRFYESMGYRTLVRLPGYYQGIEPAVRMGRRLTVTGS"
     gene            complement(2982..3521)
                     /locus_tag="VMZ02_10160"
     CDS             complement(2982..3521)
                     /locus_tag="VMZ02_10160"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR72342.1"
                     /translation="MTSLLPGLIETERLRLRQPRVTDAEIIFESYATDPEVCRYMIWQ
                     AHESVDITRRFIGECIASWRAGDRLPYVMVEKSSRTLVGMLEARSQGHSVEIGYVLAR
                     SHWGKGFMTEAVKAFTNYALAQPLVFRVQATCDIENTASARVLEKCGFVREGRLGRYL
                     IHPNISSEPRDCFIYAQVK"
BASE COUNT          804 a          984 c         1035 g          803 t
ORIGIN      
        1 ctcggcctag cgagataaag cgcgtccggg tgagcgttca agggttcaag tcattggtta
       61 gttctaccgg atatattatc gagcgaagga tagaccagtc aaaaccggcc ggtgacgatc
      121 gtccatcctc aatcttcgat cttcgactaa ttgagttcca gccccacgat cttcgccgga
      181 ttccgcgtgc acacggtata gagctcttcc tgattcagcc caaaggcttc catgtagccg
      241 cgcaccaggc gcatggcttc gacggagtcg gggaagagcg gctcgcccgc gtcgctagag
      301 agggtgaaat gctcgacgcc gacctcacga atcgtgttgt acatgatctg gggatctacc
      361 ccgagaagag gcgagagttc gtcccaggtg aagttgaaca gcaccccggt gccgcagatt
      421 tctttcatca tgtctggcga tacattcaaa aatggactga atggatgatc gatgacggcg
      481 cgtttcaagc cgactttctg tacctcttcg gcgagcttgt aaatctcagc gtgagtcgcg
      541 tggccgaaaa atagcgcgac gttgcgatcc gcgcagagat ggacgatgtc gcgaatctcc
      601 ggcttcaact tcccgttatc gtctaaatcg taatgaccgg tctttagcgc ttcgtcccac
      661 gggatcggtc caacccagcc ggcgttttcg cggccgcgta cgaaccggcc gtaggtaccg
      721 acagtgctca tagtgatcac atgggtaaag accggcagcc aaatcgccgt gacgccatgg
      781 tcgatcatgt cgctgacgat ttttgccgtc accggcgcca tttgtctgcc gcaaacgtac
      841 gccgaccaac aagtgattgg ttttacaccc tcttgttttt cggcccaacg attgacgtcg
      901 tcctgaacgg cttgggtcgc tttcaccgga tcgtcgccag gaatactttt aaataacagt
      961 cctttcattt cagctagcga agcgcgccgc gccagatcga agggatcttg ctggccttcg
     1021 tgagcatggc agtgaatatc gatggcgtct ttgactttga tatgcggcgg atgaaccgtg
     1081 cctggtttgt attgcgtgcg tgcggcatag gatgtgacgc agcgttcatg ggtcggtgtg
     1141 gaaacgttat gggtgctcat ggatttccct ccgtggctgg actggcgatg atgttgctac
     1201 aatccggacg acaatgtcaa aggcaatggg cagaagtagg cgcgccttaa gacacgaggc
     1261 gcgggtcaga tcaattcaat ttggttttaa tgggcgattt catgtaagaa cttggcagtc
     1321 tgattgagcc taatggagga ccgatgatgc ttcaacattt cgcgcgactc gcgcattgcg
     1381 cttgtcccat ggtgcttctc gtttcgttgc tgccgtggga aattctcgcg gcggccgacc
     1441 gggtcaacat gatttacacc gcgcgggtca tgtcgcaggc ctatccttgg attgctcagg
     1501 aagcgggact gttcaagaag tatgatttgg atgtgccgat ggtattcgtc acgccgggag
     1561 ctccttccgt agcggctata ctgtccggcg atagcgaggt cgccgtcatc ggcgcggcga
     1621 gcattacccg tccctgggtg caaggaaaca aggatccggt ttttgtcggt ggcatcaaga
     1681 gcttgttaac ccacagtctt atcgccaaat ccgatatcgc acgccctgaa caattaaagg
     1741 gcaaacgcat cggcgtgtcg cgcatcggca gcaatccgca ttatttcgcg gtgcaggcgc
     1801 tccgccatta caacatcgat ccgcgcgagg tgagtttcat acaagccggc ggtgcgcccg
     1861 aaaccctcgc ggcgttggtc gcgcagggca tcgatgccgc ggtattaacg gttccgaccg
     1921 acgcacaggc gctcaaactc ggttaccact acgttgtcta tggtcccgac ttaaagatcg
     1981 cctacgcggc gacgaccttt atcacgcgtc gcacgattgc cgccaagcgc ggtccggtga
     2041 ttggccgctt catgcgcgcc atggccgaag ccgcgcgtat tatgcatgtt gaccgagaat
     2101 tcacttacaa ggtcctggaa aaatacctga gagtcgacga tcgcaagcta ctggaggcaa
     2161 gctacaacgt ggagatcaaa gccttggagc cacgcctggc gatgaagccc gaaggctttc
     2221 agtccaccct ggatgaaatc gctcccactg agccgcgcgc aaagacagtc aagccacagg
     2281 aaatgatgga gacgcgctat cttgatgaga tggagaaaag cggcttcttt gagcaaattt
     2341 ggggaaggaa ataggcgtga ggcttgaggc caaaggcgcg agttgacgcc agcgcgaaat
     2401 gatttttagc aggtagttga agaaggcttg cgattcgcga acgcactatc gacgctagga
     2461 tccggtaacc gttagtcgcc ggcccatgcg cacggcgggc tcgataccct gataataacc
     2521 gggaagtcgg acgagggtgc gataacccat actttcatag aagcgctggg cgccggtgtt
     2581 gccggcgcgg acttcgagcg agatggcgac gtttcccgcg accgacgcgc aacgttccaa
     2641 ccagtgtagc aattggcgtc cgatgccggc gcggcggcag tcgggggcga cagcgaaaag
     2701 attaagatgc gcgacccgtt cgccgtattt cataatgcca aaaccggcga gtcgattatc
     2761 gaggcgggcg accagcacat tgatcgcggg atcatcgatg tttgcggcta cccgctgctc
     2821 cgtccagcgc cagtccaagc cgctctcgat caagtcgcgt gacatattgg caatggcctt
     2881 ggcgtcagca gcgcgcgcta accgtagagt tgattcggaa tgcttcatcg ccgatttacg
     2941 ccagaacagc cagactcact gaatcgccca ttgcagagct attatttgac ctgggcataa
     3001 ataaagcaat cgcgcggttc agacgagata ttggggtgaa tcaagtagcg gcccaaacgg
     3061 ccttcgcgga caaatccgca tttttccaga acgcgcgccg aagcagtgtt ttcgatatca
     3121 caagtggctt gaacacgaaa aactaacggt tgcgccagag cgtaattcgt gaacgccttt
     3181 actgcctccg tcatgaaacc ctttccccag tgcgagcgtg ccaggacata gccgatttcg
     3241 acactatgac cctggctgcg ggcttcgagc attccgacga gtgtccgcga cgatttctcg
     3301 accattacgt aaggcagacg gtcgccggct cgccaactag caatgcattc gccaatgaat
     3361 cgtctcgtga tgtcgacgga ttcgtgcgcc tgccaaatca tgtagcgaca gacctcggga
     3421 tcggtggcgt aagactcaaa gatgatttcc gcgtccgtga cgcgtggttg tcgcaggcgc
     3481 aaccgctcgg tttcaatcag gccgggaagc aggctcgtca tgccgacact gaaaacgctg
     3541 acttgagtat tgaaaacgtc agcatctcct tgtgaaactc ccaccaattg tttttcgttc
     3601 taaagctcgt cagttgcgtg tgcaac
//
LOCUS       DATLCH010000343         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10184774, whole genome shotgun sequence.
ACCESSION   DATLCH010000343 DATLCH010000000
VERSION     DATLCH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10184774"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VMZ02_10165"
     CDS             <1..440
                     /locus_tag="VMZ02_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HUR72343.1"
                     /translation="MDFMRRLGRQGIRPVDGFAHMTNLLAAGEYPLAIFMQVSKIDAL
                     KKKGAPVAWMPTSPTLATISTVGITKAAPHPASARLLMDFYLSAEGQRALVRAGKIPA
                     RRGVKSPSEEIDRLLDSANLHVLKTEGEYSRYMKLYNEVLAVR"
     gene            483..1199
                     /locus_tag="VMZ02_10170"
     CDS             483..1199
                     /locus_tag="VMZ02_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR72344.1"
                     /translation="MAVAVNGLDKVWQELVRLCNEQFESQPFKRLLEMKFTKPRAQQY
                     SIQMAYYVQNRRDCWGYVQGSAPLPVKQIIWRHEEDELIGQKAQGKVDHITLAVKEGE
                     VFGLSADAFETTARLEGGEVCFSAWIRLSQKSWLEAIAASAILEMRNSAELIRGGSLS
                     HRIGMIFERDLGIPMKKQINNAEHVVMDVEHAHLLMQVAKAYATTASAQEAILSGARE
                     SLMIDRVYRGHLAELMESLH"
     gene            1235..1939
                     /locus_tag="VMZ02_10175"
     CDS             1235..1939
                     /locus_tag="VMZ02_10175"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR72345.1"
                     /translation="MNLVKDLCAELDQMVNDQFDSIEFKAFLGVPLTLERGRFYVIQN
                     ALYTSNRRDCWGYVQGGAPLEVKRVVWEHESDELINDPRAGMDHYNLTVKQGEVIGLK
                     REDFDNAQVPAMAQACFHAWHHIAMRGHWLTSYAASHMLERRNNGKIVKGGGMSFRVG
                     KKFENELGINLKRMISLDVHVVADTEHSENISEMFEKYVKTPQDCERVLEGARESMAI
                     DRAYRGALGFYMERIN"
     gene            1974..>2289
                     /locus_tag="VMZ02_10180"
     CDS             1974..>2289
                     /locus_tag="VMZ02_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72346.1"
                     /translation="MKNLNLNEASAYSAKSPYEIYQEWEGIPVEKGFIVPDLLALELG
                     DWQRSGGKAAFVNQDGAGGMCDAMVEEIPAGGQLKPQRHMYEKAVFILQGQGATTIWN
                     EGG"
BASE COUNT          545 a          556 c          703 g          485 t
ORIGIN      
        1 gtatggactt tatgcgtcgc ttgggcaggc aggggattcg tccggtggac ggcttcgccc
       61 acatgaccaa cttacttgcg gccggcgaat atccgttggc gattttcatg caggtttcca
      121 aaattgacgc gctcaaaaag aaaggcgcgc cagtggcttg gatgccgact tctcccacgc
      181 tggcgacgat ctctaccgtt gggataacca aagccgcgcc ccatccggca tcggcgcggc
      241 tgctcatgga cttttatctt tccgccgagg gacagcgggc tttggtgcgt gccggcaaga
      301 ttcccgcgcg gcgcggcgtc aagagcccat cggaagaaat cgatcggtta ttggacagtg
      361 caaacttgca tgtgttaaaa actgagggcg agtacagtcg ttatatgaaa ctgtacaatg
      421 aagttctcgc cgtccgctga aggactgtcc gcaagagtaa acaggagtca ggagtggaaa
      481 aaatggctgt ggccgtgaat ggtttggata aagtgtggca ggagttggtc cggctgtgca
      541 acgaacagtt cgagtctcag ccgttcaaac ggctactgga aatgaagttt accaagccgc
      601 gggcgcagca atattcgatc caaatggcct actacgtgca aaatcgacgc gattgttggg
      661 gctacgttca gggctcggcg ccgctgcccg tcaagcaaat tatctggcgc catgaagaag
      721 acgagttgat cggccagaag gcccagggca aggtggacca tattactttg gctgtcaaag
      781 aaggcgaagt cttcggtcta agcgccgacg ccttcgaaac gacagcgcga ctggagggtg
      841 gagaggtttg ttttagcgca tggattcggc tgtcgcagaa gtcatggctc gaagcgatcg
      901 ccgcctcggc gattctggaa atgcgcaatt cggctgaatt aattcgcggc ggatcgttgt
      961 cgcatcgcat cggtatgatc ttcgaacggg atttgggcat tccgatgaaa aagcagatca
     1021 acaacgccga gcatgtggtg atggatgtcg aacacgcgca tcttctgatg caagtggcca
     1081 aggcatacgc cactaccgct tccgctcagg aggcgatctt gagcggtgcc cgtgagagcc
     1141 tgatgatcga tcgagtttat cgcggccact tggccgaact gatggaaagt ttgcactaac
     1201 gattcacgag tagcggtagt aagatggagg caagatgaat ttggtgaagg atctttgcgc
     1261 tgagctcgac caaatggtca acgatcagtt tgacagcatc gagttcaaag cctttctcgg
     1321 cgtgcccctg actctcgagc ggggccggtt ctatgtgata cagaatgcgc tatacacgag
     1381 caaccggcgc gattgctggg gctacgttca aggcggcgcg cctctggaag tcaagcgcgt
     1441 ggtctgggag catgagagcg acgagttgat caatgacccg cgcgccggca tggatcatta
     1501 taatctcacc gtgaagcagg gtgaggtgat cggactcaag cgcgaagatt tcgacaacgc
     1561 tcaggtgccg gcgatggccc aagcctgttt tcatgcctgg catcacatcg ccatgcgcgg
     1621 tcactggctg acatcctatg cagcgtcgca catgctcgag cggcgtaata atggcaagat
     1681 cgtcaagggc ggcggcatgt cgtttcgcgt cggcaaaaag ttcgaaaatg aattgggaat
     1741 caacttgaaa aggatgatct cattggatgt ccatgtcgtt gccgacaccg aacactcgga
     1801 aaatatttcc gaaatgttcg agaagtacgt caagacgccg caggactgtg agcgggtact
     1861 ggaaggcgcg cgcgagtcca tggccatcga ccgggcttac cgcggcgcgc tcggctttta
     1921 catggagcga atcaattaat ttcggctcgg tggagaatca aggagaacca acgatgaaga
     1981 acctgaattt gaacgaagcc tcggcatact cggcaaaaag tccgtacgaa atttatcagg
     2041 aatgggaagg gattcccgtg gaaaaaggtt ttatcgtccc ggatttgttg gcacttgagt
     2101 tgggcgattg gcagcgcagc ggcggcaaag ccgcgttcgt caatcaggac ggcgccggtg
     2161 gtatgtgcga cgctatggtc gaagagattc cggccggcgg ccaactcaaa ccgcagcggc
     2221 atatgtatga aaaggcggtt ttcattctgc agggccaggg cgcaacgacg atttggaacg
     2281 aaggcggca
//
LOCUS       DATLCH010000344        10822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7941645, whole genome shotgun sequence.
ACCESSION   DATLCH010000344 DATLCH010000000
VERSION     DATLCH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7941645"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VMZ02_10185"
     CDS             <1..689
                     /locus_tag="VMZ02_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR72347.1"
                     /translation="VRHPKTIRWHDTVLIAEEAGVNALPIVALLSFLVGVIMAFQSAV
                     ALRQFGAEIFVANMIGLSVLREMGPLMTAIILAGRSGSAFAAEIGTMKVREEIDALKT
                     MGLEPVRFLVVPRVLAAVVMTPVLTVFADLLGVMGGSVVMMSLGFPLITFFHQLQLAV
                     SYGSFVGGLVKAFAFGILIAAVGCLRGLQTETGASAVGLSTTHAVVSGIILIVITDGV
                     FTVIYYYLWV"
     gene            697..1470
                     /locus_tag="VMZ02_10190"
     CDS             697..1470
                     /locus_tag="VMZ02_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HUR72348.1"
                     /translation="MTSGDTLITVENFTAAYNGAVILNKLNFEVYRGEVFVILGGSGS
                     GKSTLLKHMIGLYPPAAGTILIDGNNVSTAEGSERQQLLRKFGVMYQSGALFGSMTVL
                     DNVCLPLQEFTDLPPDAVRFIASMKLQRVGLGGFDNHMPSELSGGMQKRAALARAMAL
                     DPQVLFLDEPSAGLDPITSAELDELILSLARSLKITFVIVSHELASIFAIADRVIMLD
                     KSVKGIVATGKPQELRDHSENPWVRQFFSRQPSADQPAH"
     gene            1507..2565
                     /locus_tag="VMZ02_10195"
     CDS             1507..2565
                     /locus_tag="VMZ02_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HUR72349.1"
                     /translation="MEKATYFKIGLFIIVGTVIAAFGVVALGMGTIFQKKIIVETYID
                     ESVQGLDVGSPAKFRGVPIGKVETISLTSAEYPTKRRYVLVRIGLTTNVFQAPLADAG
                     SPSFMTEVEKGLRVRLASQGLTGAAYIEADYQDPARNPPLEIDWQPRYPYVPSTLSRI
                     TQLSESLDKILQSVETIDIGRLVGGLEQSLVTITKVAEGANFDKLGGQANAFLTEVRE
                     TNRQLKQLIGSNDIKSAFRDSAAAASGAREMIERAQGPVNQMLADLPRAAGSLERMVS
                     RLDSVTADLPETSAQLRQTLQRMNRLIATQQQGIAATVENLQAISNDMKELTESSRKY
                     PSQVLFGAPPTPSKVMQR"
     gene            2562..3176
                     /locus_tag="VMZ02_10200"
     CDS             2562..3176
                     /locus_tag="VMZ02_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HUR72350.1"
                     /translation="MKSLRIHSAAAALFAILGSGCVNLERSYPDKRFYVLEVRPPATT
                     GGPINNEILQVSPLRISPRYQDRSFVYRISDSGFEADYYNQFIAPPAGLISEEIRKSL
                     TGAQLFKHVISASSSQAPSYVLEGTVNGIYGDFRNSNAPAAIIDLEFFLTSDLPSKPG
                     ILLQKRYVQSVPVSSRSPEALVKSWDRALEQILAALAADLAAVK"
     gene            3325..4404
                     /locus_tag="VMZ02_10205"
     CDS             3325..4404
                     /locus_tag="VMZ02_10205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 61 protein"
                     /protein_id="HUR72351.1"
                     /translation="MTGFLFGDIAPRGENVFRLTISERRRRVFRYCRDALHAVVFRHN
                     AQADASKSFSKISREQHTPFLKSQGPPEKKFETIQWADHGVREVWKLRSVRISPETGK
                     PSGYLWHYGHFIHDLIMPLADWLVEGNIDPEAIDLYIEHTPEQSVGPYVDLIKSLLGV
                     RAELLAVQSFKDLDGKELVLHAYLFGPYKRSSLDNVQQLVLSRFDLRPAAAISPEVIL
                     IERGHYPNGFENNREIPESSKKNGKQRRMIVNHDELSAALDARYGRRFCGVVLEEIPV
                     SEQIRLFHHARVVIAQHGGGLNNIIWMKGNDGVVVELAPTAIRTFQNLCLAKGLTYRT
                     LGPMNKRMPRIDSAELLRLLDSLGC"
     gene            4398..5681
                     /locus_tag="VMZ02_10210"
     CDS             4398..5681
                     /locus_tag="VMZ02_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR72352.1"
                     /translation="MLTSAPVVVVGGGPGGIAAALALGSDGLLLESAQALGGLSMSVS
                     FAGAVFDLGGHSFHTPHPHIRELVFDALDMFEQERDARCYHEGEWIPYPFQKHFTELS
                     CAETVADCANGMATIDSDIQPAHFEEQLVARFGAGLARHFMLPYNRKLWGDDLARMDS
                     AWTSERIPSARGGGSDQFSETSGVRTPLQESTRVAYPARGGFAEIFRALAERVHAVRL
                     GVEVIQIEPRQRIVYARNGDTYRYERMISTLPLPRLMASIHGVPPEILSAVMELEALG
                     LDLILIAVDEPVQTTAQRIYSAEAHIPAHKIVINHNSSGWLRTLPAHGIVAEVSRAQA
                     YGQLDRSLERTVVENLVKMGILRDPSLVKEVRQLHVSHAYPVPTHERPQIVQRAKDWL
                     TAQDIFTLGRFGEWAYINSDEVLHRGLQLGRACRD"
     gene            complement(5710..8112)
                     /locus_tag="VMZ02_10215"
     CDS             complement(5710..8112)
                     /locus_tag="VMZ02_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HUR72353.1"
                     /translation="MSKPAEQGIETKTVRGYCALCTAHCATIATVENGRVTKLEPDHD
                     HPNGGVMCIKGKAAPELVYNPERLNYPLKRTRPKSAADPGWQRVTWEEALDDIAQRLL
                     AIRERHGAKSMALGKGTKSGTSVDDVERWLGRFLCLYGSPNWVSTTHVCNWHKDTGFS
                     FTFGTTIQTPDVAHSKTFLLWGHNPSSTSLILAEDIIRARQRGMKTVVVDPRRVGIGT
                     KADILLQVRPGTDGALALALIHCLIEESWYDVEFVRRWTNGMFLLDTATGAVITEADL
                     SANGDASCFVVWDEAKNQAVLYQSANGAYERDDVRPALFGTHVLKGKDGREISCKPVF
                     EKLGDIAAEFAPEKSSKITWVPAEQVWKTALMLAHNRPVSMYMWNGLGQHTNATQTSR
                     AIASLYALLGDFDRKGGNVTFPKVPTNNVDGKEFLSKEVAALRIGRDKKPLGPPASPG
                     NCTAYDIFTAILDQQPYAIKAFLAFGCNPVMSNADPRRARAALSKVEFAVALDLFMTP
                     TAALCDYVLPVTSFLEMSSITTAFEHRQLGKTHLQYRPAVLEPLYERRSDTWILFELA
                     KRMGMGEQFFDGDIEAGYAYELEPTGITLDELKNTPGGISRPIAPTFEKHAKVDKHGK
                     IRGFATSSTRVELYCHKFAAAGYPALPEYIEPALSPVSRPDVAADFPLVLTNAKNTTF
                     VHSQQRALTSLRKALPEPCADIHPETALRYGVKHKEWMVVETPKGAIKVKARVTKNIV
                     PGTICVQHGWWQGCKELELPGYNVYDETGANAAMLIGTDQADPISGSLPHRSYLCRVR
                     PT"
     gene            complement(8223..9704)
                     /locus_tag="VMZ02_10220"
     CDS             complement(8223..9704)
                     /locus_tag="VMZ02_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HUR72354.1"
                     /translation="MPNSNIETDVAIIGGGGAGIPAAIEVARAGAKALVLEGAAECGG
                     TAAISGGGCCIVGTPLQKSQGINDTPDMAFDDWINWGGGQADEVWARYYLEHSLHDLY
                     HWADRCGVRWVDLKFQEGNRVLRWHRPENNGLGLMTALIKTAETMPNITIMRSTKAGK
                     LITKDGRVAGLTATKENQTWDIHSKTVVVATGGFNSNLDMVLEHRPEFKNFRVMEGSG
                     FGAKGEGHTMIRELGGYFTHMDCIWFYVYATPDYRDPKQRRGLVFRMVPGYIWFNQQG
                     RRFHNESLTGGASATPKLLAQTPPHAWAIADTPMLAKIDVADPYYRRGDVIDRAKVEE
                     LLNNSPFIKKADSFEELGQQCGFDDLKNFVTEVEEYNQCFEAKLDKDSKFGKSLKGSK
                     KFDTPPYYAIQICPLARKNFGGAKTDLRCRALNKYFEPIPGLYAAGEVAGMAGGHING
                     KAGLEGTMLGPSIFSGRVAGAWAAHEAGFGPGFIGKPNRPELD"
     gene            9842..>10822
                     /locus_tag="VMZ02_10225"
     CDS             9842..>10822
                     /locus_tag="VMZ02_10225"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72355.1"
                     /translation="MIRRRLGPFLLMSVALLTFASGNGHSQENVRIAHPSLSASVMCL
                     MIANKEGYFREEGLNVEFLSIRGEIAIRTTLAGEIDMFTNAGSAINAVARNVPLKILA
                     VFQDKPGWDLIALPNFKSVAQLRGQTVAIMSPEGSLAVVTREILKKHGIDAAKDAQLV
                     VMGGDDVRFPALKAKAIQATLFNTAASLRAQKDGFVKLAAAGEYVNTIQGGLATSDDK
                     IKQQPAKIAKIMRAALKGAAFYLTKREATIKYSMDILKLKDRDMAAAIYDEEAKLMVR
                     NGISDAKILQPMIDEMKAATKTPRDMKFADVFDFSFARKANEELKASGWKP"
BASE COUNT         2274 a         2929 c         3124 g         2495 t
ORIGIN      
        1 ctgtgcgtca tcctaagaca attcgctggc acgacacggt gctcatcgct gaagaggccg
       61 gagtcaatgc attgccgatc gtcgccttac tgagttttct cgtcggcgtc atcatggcgt
      121 ttcaatcggc agtcgccttg cgtcagttcg gcgcggagat ttttgtcgcc aatatgattg
      181 gcttatccgt gctgcgtgag atgggaccat tgatgacggc gattatcctc gccggccggt
      241 cggggtcggc ctttgccgcc gagatcggca ccatgaaagt gcgcgaggaa atcgacgcgc
      301 tcaagaccat gggcctggag ccggtgcgat ttcttgtagt gccgcgagtg cttgccgccg
      361 tcgtaatgac cccggtgcta accgtcttcg ccgatctgtt gggggttatg ggcggctctg
      421 tcgtcatgat gtcgcttggg tttccgctga ttactttttt tcatcaattg cagttggcgg
      481 tgagctacgg ttcgtttgtc ggcggcttag taaaagcctt cgccttcggc attctgatcg
      541 ctgccgtcgg ttgcttacgc gggcttcaga ccgagaccgg cgctagtgcg gtaggacttt
      601 cgaccaccca cgccgtggtg agcgggatta tcttgatcgt tattaccgac ggggttttta
      661 cggtgattta ctattatctt tgggtttgag ccgattatga caagcggtga tacgctcatt
      721 acagtcgaga atttcaccgc agcttataac ggcgcggtga ttctcaacaa actgaacttc
      781 gaggtgtacc gcggcgaagt atttgtcatt ctcggcggct cgggctcggg caaaagtacg
      841 ctgctcaaac acatgatcgg cttgtatccg ccggcggccg ggacgattct catcgacggt
      901 aacaatgtca gcacggccga agggagtgaa cgccagcaac tgttgcgcaa gttcggcgtg
      961 atgtatcaaa gcggcgcgct gttcggttcg atgacagtac tcgacaacgt ctgtttgccc
     1021 ctccaagaat ttaccgattt gccgcccgac gcggtccgat tcatcgcctc gatgaaactc
     1081 cagcgcgtcg gtctcggcgg ttttgacaac catatgccgt cggaactaag cggcggtatg
     1141 caaaagcgtg ccgcccttgc tcgcgccatg gcactcgatc cgcaggtgtt gttcctcgat
     1201 gaaccgtcgg ccggtctcga tccaattacc tcggcggagc tcgatgaatt gattctctcg
     1261 ctggcgcgca gtttgaaaat cactttcgtc atcgttagcc acgagttggc gagtattttt
     1321 gccattgcgg atcgggtgat tatgttggat aaaagtgtca agggcatagt cgcaaccggc
     1381 aaacctcaag aactgcgcga tcacagcgag aatccctggg tgcgacaatt tttttctcgg
     1441 cagccttccg ccgaccaacc cgcacactaa ttcggggaaa ttttctaacc atctggaccc
     1501 acagatatgg aaaaagccac ctactttaaa attggtctgt tcatcatcgt tggcaccgtt
     1561 atcgccgcct tcggcgtcgt cgcgctcggt atgggtacga tctttcaaaa gaagattata
     1621 gtcgaaacct atatcgacga atcggtgcag ggactcgacg tcggctcgcc cgccaagttt
     1681 cgtggtgtgc cgatcggcaa ggtcgaaacc attagcctga ccagcgccga gtacccgacc
     1741 aagcgtcgct atgttttggt ccgtatcggc ctgacgacga acgtttttca agctccgctt
     1801 gctgatgccg gctcgccgag ttttatgacc gaggtcgaaa aaggcctgcg cgttcgtctc
     1861 gcatcccagg gactcaccgg tgcggcctac atcgaagcgg attatcagga tccggcgcgc
     1921 aatccgccct tggagatcga ttggcagccg cgttatccct atgtgccgtc gactttgagc
     1981 cgaattaccc agctgagcga gtctttggat aaaattcttc agagcgtgga aaccatcgat
     2041 atcggccgcc tggtcggtgg cctcgaacag tctttggtga cgattactaa agtcgccgaa
     2101 ggagcgaact tcgacaagct cggcggtcag gccaatgcct ttttgaccga ggtgcgcgag
     2161 accaatcgcc aattgaaaca actgatcggc agcaacgaca taaagtctgc ctttagggac
     2221 agcgcagccg cggcgagcgg cgcgcgcgag atgatcgagc gcgcgcaagg gccggtcaac
     2281 caaatgctcg ccgacttgcc gcgcgcggcc ggaagtttag agcggatggt gtcgcggctc
     2341 gattcagtca ccgccgattt accggagacc agcgcgcaac tgcggcagac cttgcaacgc
     2401 atgaaccgat tgatcgcgac tcagcaacag ggcatcgctg ctacggtcga gaacttgcaa
     2461 gcgatatcca acgacatgaa agagctcacg gaaagttcgc ggaagtatcc gtcgcaggta
     2521 ttgttcggcg cgccgccaac cccgtccaag gtgatgcaac gatgaaatca ttgagaattc
     2581 attctgcggc cgcggcgctg ttcgctatac tcggcagcgg ttgcgtcaac cttgaacgca
     2641 gctatcccga caagcgtttt tatgtcttgg aagtgcgccc gccggcgacg accggcgggc
     2701 cgatcaacaa tgagattttg caagtgtcgc ctttacgtat ctcaccgcga taccaggaca
     2761 ggagtttcgt ttatcgcatt tccgatagcg gcttcgaagc cgattactac aatcaattta
     2821 ttgctccgcc agcaggattg atcagcgaag agattcgtaa gagtttgacc ggcgcgcagc
     2881 tctttaaaca cgtgataagc gcgtcgagta gtcaggcgcc gagctatgtg ttggaaggta
     2941 ccgtcaatgg aatttacggt gatttcagaa attccaacgc tcctgctgcg attatcgatt
     3001 tggagttttt tttgaccagc gatctaccct ccaagcccgg cattctcttg cagaagcgct
     3061 atgtgcagtc ggtaccggtg agcagccgct cgcccgaagc attggtcaaa agctgggatc
     3121 gggcgctgga acaaattctc gccgccctcg cggcggatct cgccgcggtg aagtagtttg
     3181 cccgagacga gctcccgccg gggccgcatc ggccattcgg tcgttgattg tttagtccag
     3241 cgaataccgc tcgaagcact ccggaccact gtgtaaccga accacttcga gtagaccaaa
     3301 aaatttaatc aagcacttga atgcttgact ggttttctgt tcggcgatat agctccccgc
     3361 ggagaaaacg tgttcagact tacaatcagc gagcgccgac gtcgcgtttt tcgttattgc
     3421 cgcgacgcgt tgcacgccgt ggtatttcgg cataacgcgc aagccgatgc gtcgaaatca
     3481 ttcagcaaaa ttagccgcga gcagcacacg cctttcctga aatcacaagg tccgccggaa
     3541 aagaaattcg agacgattca atgggccgat cacggggtgc gcgaagtttg gaaacttaga
     3601 tccgtgcgga tttcgccaga gacaggcaag ccttccggtt atctttggca ttatggccat
     3661 tttatccatg atctgatcat gccgttggcc gattggctcg tcgagggaaa tatcgatccg
     3721 gaggccattg atctttatat cgagcacaca ccggaacagt cagtgggccc ctatgtcgat
     3781 ctgattaaat ctttgttggg tgtgcgggca gagctgctgg cagtgcagag tttcaaggac
     3841 ctcgatggta aagaacttgt tctccatgcc tatctttttg gtccctacaa gcggtcgtcg
     3901 ctcgacaatg tgcaacagct agtcttgtct cggtttgatc ttcgcccggc tgcggcaatt
     3961 tcgccggagg tgattctgat cgagcgtggc cattacccga acggttttga gaacaaccgg
     4021 gaaattcctg aaagtagcaa aaaaaacggc aagcagcgtc gcatgatcgt caatcacgac
     4081 gaactctcag cggccctcga tgcgcgctac ggacggcggt tttgcggagt ggtgcttgag
     4141 gagattcccg tgtcagaaca gataagattg tttcaccatg cccgagttgt tatcgctcag
     4201 cacggtggcg gtctgaataa tattatctgg atgaaaggca acgatggggt ggttgttgaa
     4261 ctcgctccta ctgcaatcag aacatttcaa aatctctgct tagccaaggg actgacctat
     4321 cgcacgctag gtccgatgaa taaacgaatg ccacggatcg actccgccga gttactgcgc
     4381 ctgctggatt ccttgggatg ctgacatcgg caccggttgt ggtggtaggg ggtggtcctg
     4441 gaggcattgc ggccgcattg gcattgggct ccgatggatt gttgctggaa agtgcgcaag
     4501 cgctcggcgg tctgtcgatg agtgtgtcat tcgctggcgc cgtgttcgat ctcggcggtc
     4561 attccttcca tactccgcat ccgcatatcc gagagttggt ttttgatgcc ctagatatgt
     4621 ttgaacagga gcgcgatgcc cggtgttatc atgaagggga atggatccct tatcccttcc
     4681 aaaagcattt caccgagctc agctgcgccg aaacggtagc ggattgtgcc aacggcatgg
     4741 cgacgatcga cagtgatatc caaccagccc atttcgagga gcaactcgtg gcacgtttcg
     4801 gtgccggcct cgcccgccac ttcatgctcc cttacaaccg taagctctgg ggcgacgatc
     4861 tcgcgcgcat ggatagcgca tggaccagcg aacgaattcc cagcgcaaga ggcggcggca
     4921 gcgaccagtt ttctgaaacc agcggtgttc gaacgccgct ccaagaaagc actcgggtcg
     4981 catatcctgc gcggggcggc ttcgcagaaa tcttccgagc gctagccgag cgcgttcacg
     5041 ctgtgagact tggcgtggag gtgattcaga tcgagccgcg ccagcggatc gtctatgcgc
     5101 gtaacggaga cacttatcga tatgaacgaa tgatttccac tctgcctttg ccgcggctaa
     5161 tggccagcat ccacggagtg ccgccagaaa tcttgtcggc agtgatggaa ctggaggcgc
     5221 ttggacttga cctgatcctg atcgcagtgg atgaacccgt tcagaccaca gcgcaacgaa
     5281 tttacagcgc cgaagcgcac atcccggcgc acaagatcgt aatcaaccac aactcatccg
     5341 gttggctgcg gaccttgccg gcccatggca ttgtcgccga agtgtctcgt gctcaggcct
     5401 acggacaatt ggaccggtcg ctggagcgta ctgtcgttga aaacctggta aaaatgggga
     5461 ttctgcgtga tccgtcccta gttaaggaag tgcgccaact gcatgtttca catgcctatc
     5521 cggtgccgac tcacgagcgc ccgcagatcg tgcaacgggc caaagactgg ctaactgctc
     5581 aggacatttt cactctcggg cgcttcggag aatgggctta tatcaactcc gacgaagtac
     5641 tacatcgagg tctgcaactg ggacgtgctt gtagagactg actgcggcgc attaagctca
     5701 taaaaaaact cacgtaggtc tgacccggca cagatacgaa cgatggggca gtgacccgct
     5761 gatcggatca gcctggtcgg tgccgatgag catggcggcg ttggcgccgg tttcgtcgta
     5821 gacattgtag ccgggaagtt ccaattcttt gcaaccttgc caccagccat gttgcacgca
     5881 gatggtgcca gggacgatgt ttttggtcac gcgcgctttg actttgatcg caccttttgg
     5941 agtttcaacc accatccatt ctttgtgctt aacgccgtaa cgcaatgcgg tttccgggtg
     6001 aatatcggcg cacggttcgg gcagcgcttt gcgcagacta gttaacgcgc gctgttggct
     6061 gtgcacgaaa gtcgtgttct tggcgttggt gaggacgaga ggaaaatcgg cggcgacgtc
     6121 aggccggctg accgggctca gagcaggttc gatatattcc ggcagtgctg gatatccggc
     6181 ggcggcgaat ttatggcaat agagttcgac tcgcgtcgac gacgtggcga agccgcgtat
     6241 tttgccgtgc ttgtcgactt ttgcatgctt ctcgaaggtc ggagcgatcg gacgggaaat
     6301 gccgcccgga gtgtttttta actcatcgag agtaataccc gtcggttcga gttcgtaggc
     6361 gtaaccagct tcgatgtctc cgtcgaagaa ttgctcgccc atacccatcc gcttggccag
     6421 ctcgaaaagt atccaggtat cagagcgccg ttcgtacaaa ggttcaagca cggcggggcg
     6481 atattgtaga tgggtcttgc ctaactgtcg gtgttcgaat gcggtggtga tcgacgacat
     6541 ctccagaaag ctggtgacgg gcaaaacgta atcgcacagt gcggccgtgg gtgtcatgaa
     6601 cagatccagc gccaccgcga actctacttt gctcagcgct gcgcgggcgc ggcgtggatc
     6661 ggcgttggac atgaccggat tacagccgaa ggcgagaaac gctttgatcg catacggttg
     6721 ttggtcgagg atggcagtga agatgtcata ggcggtgcaa tttccgggcg atgccggcgg
     6781 acccagcggt tttttgtcgc ggccaatgcg cagcgccgct acttctttcg agagaaactc
     6841 tttgccgtcg acgttattgg tcgggacttt aggaaaggtc acgttgccgc ccttacgatc
     6901 gaagtcacca aggagcgcat agagcgaagc gatggcgcgg ctagtttgcg tcgcattagt
     6961 gtgttggccg agcccattcc acatatacat gctgaccggc cggttgtgcg ccagcatgag
     7021 tgcggtcttc cagacttgtt cggcaggcac ccaggtgatt ttcgaggatt tctccggcgc
     7081 gaattcggcg gcaatatcgc cgagcttctc gaaaaccggt ttgcagctta tttctctgcc
     7141 gtctttgccc ttgagaacgt gcgtaccgaa gagtgccggc cggacgtcat cacgttcgta
     7201 tgcgccgttg gccgactgat aaagaactgc ttggttcttc gcctcgtccc atacaacgaa
     7261 gcagctggca tctccgttgg cgctgagatc cgcttcagta atgacggcgc cggttgccgt
     7321 atctaacaag aacatcccat tggtccagcg gcgtacgaac tcgacgtcgt accaactctc
     7381 ctcgatgagg caatggatca gcgcgagcgc cagtgcgcca tcggtgcccg gacgcacttg
     7441 caagagtatg tccgccttag taccgatgcc gacgcggcgc ggatcgacga ccacggtctt
     7501 catgccgcgt tggcgtgccc ggatgatatc ttccgcaaga atcagcgagg tcgagctagg
     7561 gttatgaccc cagagaagaa acgttttgct gtgagcgaca tcgggggttt gaatggtcgt
     7621 gccgaaagtg aaactgaaac cggtgtcctt gtgccagttg cacacatgag ttgtggaaac
     7681 ccagttggga ctgccgtaga gacaaagaaa gcggccgagc cagcgctcga catcgtcgac
     7741 ggacgtgccg ctcttagtgc ctttgcccaa tgccatcgac ttggcgccgt ggcgctcgcg
     7801 gattgccaac aatctttgcg cgatgtcatc aagcgcttcc tcccaagtga cgcgctgcca
     7861 gccgggatcg gcagcgctct tgggccgcgt gcgcttgagc gggtaattta atcgctcggg
     7921 attgtaaacc agctctggtg cggctttgcc tttgatacac atgacgccgc cattgggatg
     7981 atcgtggtcc ggttccaatt tggtcacccg gccgttctcc acggtggcaa tcgtcgcgca
     8041 gtgggcggtg cacagggcgc agtagccgcg cactgtttta gtctcaatcc cctgctctgc
     8101 cggtttggac atacgttctc cgaatggtga cggcgtgtta tgcgcggtgg ttcaaggttc
     8161 aagggttcaa agttcaagga cggagttcga ccgattgaaa cttttgaacc ttgaacggct
     8221 attcagtcta actccggtcg attcggttta ccgatgaagc cgggaccgaa gccggcttcg
     8281 tgggccgccc aggcaccggc gactcggccg ctgaagatcg acggaccgag catcgtgcct
     8341 tccagtccag ccttgccatt gatgtgaccg ccggccatac cggcgacttc tccagccgca
     8401 taaagtcccg ggatgggctc gaaatatttg ttgagggcgc ggcagcgcag atcggttttg
     8461 gcgccgccaa aattcttgcg cgccagggga caaatctgaa tcgcgtaata gggtggtgtg
     8521 tcgaatttct tcgaaccctt gagtgatttg ccaaacttgg aatctttgtc cagttttgcc
     8581 tcaaagcatt ggttgtattc ttcgacttcg gtgacgaaat tctttaggtc atcgaacccg
     8641 cactgctggc caagttcttc gaaactgtca gcttttttga tgaatggcga attgttaagc
     8701 aactcctcga ctttggctcg gtcaataaca tcgccgcggc ggtagtacgg gtcggcgacg
     8761 tcgatcttcg ccagcatggg cgtgtcggca atggcccagg catggggcgg tgtttgcgcc
     8821 agcagctttg gcgttgccga ggcgccgccg gtaagcgatt cattgtgaaa gcgccggccc
     8881 tgctgattaa accaaatata gccgggcacc atgcggaaaa ccaagccgcg gcgctgctta
     8941 ggatcgcggt agtctggtgt cgcataaaca tagaaccaaa tgcagtccat gtgagtaaag
     9001 tagccgccca actcgcggat catcgtgtgt ccctcaccct tcgccccaaa accggagcct
     9061 tccatgactc ggaaattttt aaactctggg cgatgctcca gcaccatgtc gagatttgag
     9121 ttgaaaccgc cggtggctac cacgacggtt ttactgtgaa tatcccaggt ttggttttct
     9181 ttggtcgcag ttaggccggc gacccggcca tcttttgtga tcagcttgcc ggctttggta
     9241 gacctcatga tagttatgtt gggcatcgtt tcggcggttt tgatcagcgc ggtcatgagc
     9301 cccaaaccgt tgttttccgg gcgatgccag cgcagtacgc gattgccttc ctgaaatttc
     9361 agatcgaccc aacggacgcc gcagcgatcg gcccagtgat aaagatcgtg cagactatgt
     9421 tcgagatagt agcgcgccca gacttcgtcg gcctggccgc cgccccagtt gatccagtca
     9481 tcgaaagcca tgtccggcgt gtcgttaatg ccctgggact tttgtaacgg tgttccgaca
     9541 atgcagcagc cgccgccgga tattgctgct gtgccaccgc attccgccgc accttccaaa
     9601 accagcgctt tggcgccggc gcgcgcgact tcgatggcgg cgggaatacc ggcgccgccg
     9661 ccaccgataa tcgctacatc agtttcgata ttcgagttgg gcatggcgaa ctccttcgga
     9721 ccgatcgagg tggtcgatga tttatcactc ttgtcgggag cgggtcaacg cgaaccctct
     9781 tgccaagtgc gcgggctttc tgtatcggga atgtaatcgt agttcgccaa ggagattatc
     9841 tatgatccgc cgccgattgg gtccgtttct tctgatgagt gttgccctct tgaccttcgc
     9901 tagcggcaac ggccactcac aggaaaacgt tcgcattgcc catccttcat tgtcggcttc
     9961 ggtgatgtgc ttgatgattg ccaataaaga gggctatttc cgagaggagg gattaaacgt
    10021 cgagtttttg agcattcgcg gcgagattgc gattcgcaca accctcgccg gcgagatcga
    10081 tatgtttact aacgccggca gcgcgattaa cgcggtggcg cgcaacgtgc cgttgaaaat
    10141 actcgccgtt ttccaggata agcccggctg ggatttgatc gcattgccga atttcaaatc
    10201 cgtcgcgcaa cttcgcggcc agacggtggc aattatgtct ccggaaggct ctcttgcggt
    10261 ggttactcgt gagattttga aaaaacacgg catcgatgca gcgaaggatg cgcaattggt
    10321 cgtcatgggc ggcgacgacg tgcgcttccc ggcgctcaag gccaaggcga tccaggcaac
    10381 gctctttaat actgcggcca gtctacgtgc gcagaaagac ggcttcgtca aactcgctgc
    10441 ggcgggtgag tatgtcaata caatccaagg ggggttggcg acctccgatg acaagatcaa
    10501 acaacagccg gcgaaaatcg ccaagatcat gcgcgcggcc ctgaagggcg cggcgtttta
    10561 tctcacgaag cgcgaggcga ccataaaata ttccatggac atcctcaaac tcaaagaccg
    10621 ggatatggcg gcggcgatct acgacgaaga ggccaagctc atggtgcgca acggcatcag
    10681 cgacgcgaag attctccaac ccatgatcga cgagatgaag gccgcgacca agacaccgag
    10741 ggacatgaag ttcgccgacg tcttcgactt cagcttcgcg cgcaaagcga acgaagagct
    10801 aaaggcgagc ggctggaagc cg
//
LOCUS       DATLCH010000345         3935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3625396, whole genome shotgun sequence.
ACCESSION   DATLCH010000345 DATLCH010000000
VERSION     DATLCH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3625396"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VMZ02_10230"
     CDS             <1..362
                     /locus_tag="VMZ02_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HUR72356.1"
                     /translation="ALASSAKGDELTIYKAIACIDDLVEAETSSDDADKSKPFPDIFQ
                     AALAQLGDIPANEVVVVGDTGYDAEAARKANLRTVGLMSGGWSENDLIQAGCIAVYRD
                     AADLLMRYDTSPLRAVS"
     gene            complement(397..993)
                     /locus_tag="VMZ02_10235"
     CDS             complement(397..993)
                     /locus_tag="VMZ02_10235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HUR72357.1"
                     /translation="MAAPQHNDPLQHAPDDSRLALLIVDVINDLEFPGGDKLLEQALP
                     MANALSAFKRRAKQAGVPVIYANDNFGRWRSDFPKLVEHCLRDGIRGEQIVRRLAPEP
                     DDYFVLKPKHSAFYQTNLDILLKYLGSQIVIITGMASDICVLFTANDAYMRDLKVIVP
                     SDCVAAEDSANNQTVLALMERVLKAEIRRSSEINFPYE"
     gene            1315..1851
                     /locus_tag="VMZ02_10240"
     CDS             1315..1851
                     /locus_tag="VMZ02_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HUR72358.1"
                     /translation="MSKREAAPTQPDAQAALKNISSISQLEHQALQTRSLAESISDAL
                     TKAVGSFPSIVFHAGFFLAWIVINLGWVSVVPPFDPFPFGILTLIVSTEGVLLAIIIL
                     ISQNRMIRQADRRAHLDLQINLLAEQEASLILHQVRRIAERMQLPPDERDREAALLTE
                     TTDVDKVMHILEEQLPKD"
     gene            1912..2871
                     /locus_tag="VMZ02_10245"
     CDS             1912..2871
                     /locus_tag="VMZ02_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HUR72359.1"
                     /translation="MTRVVKEPKHLFDLGGLSGKELARRVFNCVQKTNCMGNAAALAY
                     YFLFSLFPFLLFLTALLAYLPVDNLLEQMLALLARFMPGEAHQLVRDYINSLLAQPRT
                     GILSFGILLALWTASNAITAICDGLNHAYGVEERRPFWKVRVVAILLTIGLSVFLIAS
                     IVLLIFGPQLGAYIANWVGLGEIFKSAWNIVRWPVILILVTTAVAVIYSFAPDVEQRW
                     KWITPGSLFAVLMWTAVSLGFSYYVNNFGSYDKTYGSIGAIIVLLTWMYASGLVILIG
                     GVINAQIDHARTTGDTLSEKRAEQPQRPQIRRVPPNGTVPRHP"
     gene            2879..3844
                     /locus_tag="VMZ02_10250"
     CDS             2879..3844
                     /locus_tag="VMZ02_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72360.1"
                     /translation="MEAVQSLSDGDDRFARWLANMRRGAFAEAWRISDQVLHERRGRP
                     CADLPPHERYFWDGTPLTDKRVMIRCYHGLGDTIQFVRYAPCVRAIAQSVRLAAQPEL
                     TPLLQPLAALGIELVDDGFADFDLDVELMELPHVFRTTLATMPRRTPYIHVQPAALQR
                     AAKLEVGLVWRAGAGWDRRRNIPGAELAPLATVPDMRLHILQNRQDCHDLPAGLGVMA
                     QRDQLFDLACFMRALDLVITVDSMPAHLAGALGVPVWNLLHSDPDWRWMEECERSPWY
                     PTMRLFRQRKIGDWRGVIAHVTSELRRLTNSSQGPSIPLPANITL"
BASE COUNT          840 a         1055 c         1100 g          940 t
ORIGIN      
        1 tcgcgctggc ttcgtcggcc aaaggcgacg agcttacgat ttataaagca atcgcctgta
       61 tcgatgacct ggtcgaagcc gagacttcgt ccgacgatgc cgacaagtca aaaccatttc
      121 cggatatatt ccaggccgcg ctcgcacaac taggcgatat cccagccaac gaagtcgtcg
      181 tcgttggcga caccggctat gacgccgaag ccgcgcgaaa agccaatttg cgaacagttg
      241 gtctcatgtc gggaggctgg agcgagaacg acttaattca agccggctgc attgccgtct
      301 atcgtgacgc agctgattta ctgatgcgct acgatacttc gcctttgcgc gccgtgagct
      361 gagcatagtc ggttcgccta acgtgcgcga atttttttac tcgtagggaa aattaatttc
      421 cgaggaacgt cggatttccg ctttgagcac ccgttccatc agcgcgagca cggtctggtt
      481 attggccgaa tcttccgctg ccacgcaatc cgacgggacg atgactttga gatcgcgcat
      541 gtaggcgtcg ttggcggtga acaatacgca aatatcactc gccatgccgg tgataatcac
      601 tatttgagaa cctaaatatt tcaacagaat gtcgagattg gtttgataaa acgccgagtg
      661 cttgggcttc aggacgaagt agtcgtcggg ctcgggtgcg agccggcgga cgatctgctc
      721 accgcggata ccatcgcgca aacaatgttc gaccagcttg ggaaagtcgg aacgccagcg
      781 gccgaagtta tcgttggcgt aaattaccgg cacgccggcc tgtttcgcgc ggcgcttgaa
      841 agcgctcaac gcgttcgcca tcggcagcgc ctgctccaaa agcttgtcgc cgcctgggaa
      901 ttccaaatcg ttgattacat cgacgatcaa caacgcgagc cgtgagtcgt cgggggcgtg
      961 ttggagaggg tcgttatgtt gaggagctgc catgccggtc gcctttcaga aatctcagaa
     1021 aaatcagaaa tctcagaaaa attcttcgaa gaaagggcag gcacggaggg agatttctct
     1081 ccccccgtgt gatgcggata aaacagagga gtagcacgag gttcgcaggc tttatggccg
     1141 tgcgatgact ctatctatca gcaaacagtg gaccagcctt gtgagcgcta tttgaaataa
     1201 aaactccgcg aaatctgtcc aaattattgg aacgtgcggt tttgctgtct cagttttgca
     1261 acgcggcttt atagaacatt tagtggcact aactttgcgg catctcagaa taaaatgagc
     1321 aaacgtgagg ctgcgccaac tcagccggac gcgcaagcag cgctcaagaa tatcagcagc
     1381 atttcccagc tcgaacacca ggcgctgcaa acgcgctccc tagcggaaag cattagcgat
     1441 gctttgacga aagctgtggg cagctttccg tccatcgttt tccatgccgg gttctttctc
     1501 gcctggatcg ttatcaatct tggatgggtc tctgtggtgc cgccgtttga cccctttcca
     1561 ttcggcattc tgacattgat tgtttcgacc gaaggagttc tgctggcaat tattattctg
     1621 atcagccaga accgcatgat ccgccaagcc gatcgtcgcg cccatttgga tctgcagatc
     1681 aatttgcttg ccgagcaaga agccagcttg attctccatc aggtgcggcg aatcgccgaa
     1741 cgaatgcagc tgccgccgga cgagcgcgac cgcgaagccg ccctgctgac ggaaactact
     1801 gatgtcgata aagtgatgca tatactcgaa gagcagttac ccaaagacta accagacccg
     1861 tttcacgtta cgagatcgtc tgcagagcga gttctaaaca aaggtcatcc tatgactcga
     1921 gtcgtaaaag aaccaaagca tctgttcgac ttgggcggtc ttagtggcaa agagctcgct
     1981 cggcgcgtct ttaactgcgt gcagaaaacc aactgcatgg gcaatgccgc ggctctggcg
     2041 tattatttct tgttttcctt gtttccgttt ctgctatttc tcacagcgtt attagcttac
     2101 cttccggtag acaatttgct tgagcagatg ctggcattgt tggcgcgctt catgccgggt
     2161 gaagcgcatc agctcgtgcg cgactatatc aacagtcttt tagcccaacc gcgcactgga
     2221 atcttgtctt ttggcatcct gctcgcgctc tggaccgcat cgaatgcgat tacggcgatt
     2281 tgcgatggtc tcaaccatgc ctacggcgtc gaggaacgca ggccgttctg gaaagtgcgc
     2341 gtcgtcgcga ttcttttgac gatcggtttg tcggtttttc tgatcgcgtc gattgtgttg
     2401 ttgatcttcg gcccgcaact cggcgcttat atcgctaact gggtgggact cggtgaaatc
     2461 tttaaatcgg cttggaatat cgtgcgctgg ccggtgatat tgattctcgt gacgacggcg
     2521 gtcgcggtga tttattcttt tgcgccggat gtcgagcaac gctggaaatg gattactccc
     2581 gggtctttgt ttgcggtgct catgtggaca gccgtgtcgt taggtttttc ttactacgtc
     2641 aataactttg gttcatacga caaaacctac ggtagtatcg gcgcgattat cgtcctgctt
     2701 acgtggatgt atgccagcgg tttggtcatt ctcatcggcg gagtgatcaa tgcgcaaata
     2761 gaccatgcca ggacgaccgg tgacacgctt agtgagaagc gcgcagagca gcctcaacgt
     2821 cctcagattc gacgtgtacc gccaaacggt actgtgccgc gccatccgta gtccaggtat
     2881 ggaagcagtc cagtcattaa gtgacggaga tgaccggttc gcccggtggc tggcaaatat
     2941 gcgccgcggc gctttcgccg aggcgtggcg gatcagcgat caagtcttgc acgaacggcg
     3001 cggccggcca tgcgccgatt tgccgccgca tgaacgttat ttttgggatg gcacgccgtt
     3061 aactgacaag cgtgtgatga ttcgctgcta tcatgggctt ggcgatacga ttcagtttgt
     3121 ccgctatgcg ccatgcgtac gcgccattgc acaaagcgtt cgtctggcgg cgcaacccga
     3181 gctaacgcca ctcttgcagc cgttggccgc cctgggcatc gaactcgtcg atgacggctt
     3241 cgccgacttc gacctcgatg tggaactcat ggagttgccc catgtgtttc gcaccacgtt
     3301 ggcgaccatg cctcggcgaa caccctacat ccacgtgcaa ccggcagcgc tgcagcgcgc
     3361 cgcgaaattg gaggttggtt tagtctggcg cgctggagcc ggttgggatc gccggcgcaa
     3421 tattccaggc gccgagctag cgccgctcgc aaccgtgccg gatatgcgtc tgcacatcct
     3481 gcagaatcgc caagactgtc atgacttgcc cgcgggtttg ggagtgatgg cgcaacgcga
     3541 ccagctgttc gatctggcgt gcttcatgcg cgctctcgac ttggtgatca cggttgattc
     3601 gatgccggcg catttggccg gtgccctggg cgtgccggtc tggaacctgc tgcacagcga
     3661 ccccgactgg cgctggatgg aagaatgcga acgcagtcct tggtatccaa ccatgcgatt
     3721 gttccgtcaa agaaagatcg gtgattggcg gggagttatc gctcatgtga ctagcgaact
     3781 gcggcgcttg acgaacagtt ctcagggccc gagcataccg ctgccggcga acatcacgct
     3841 ttaaaatctg cctgtttcta ttctgctaca agttaatcac cgttcgtccc gcgactacga
     3901 tgcgaagcgg cgcgaatggc gaaaacctgt aagtc
//
LOCUS       DATLCH010000346         5582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7943520, whole genome shotgun sequence.
ACCESSION   DATLCH010000346 DATLCH010000000
VERSION     DATLCH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7943520"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..929)
                     /locus_tag="VMZ02_10255"
     CDS             complement(51..929)
                     /locus_tag="VMZ02_10255"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HUR72361.1"
                     /translation="MKPKSKPRGPLLGAHMSIAGGVGNAFLQGQKVACQAIQIFTKSS
                     RQWASKPLDNEEIAQFHANRKSTGISAVVAHDSYLLNLGSPDPILRKRSIGAFIDEME
                     RCEVLAVSNLIAHPGAHIGAGESEGIKTIAKSLDEVHKACAGFKVKVTLETTAGQGSN
                     LGYRFEQIGDIIDATRECDRLRVCFDTEHAFAAGYDIRTAEGYERTFAEFDQTIGIEL
                     LAAFHLNDSKKELHSRVDRHEHIGKGFIGVEGFRLLMNDQRFWGLPMCLETPKGPELK
                     EDMENLTLLRSLIIEN"
     gene            complement(1033..2643)
                     /locus_tag="VMZ02_10260"
     CDS             complement(1033..2643)
                     /locus_tag="VMZ02_10260"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HUR72362.1"
                     /translation="MSKFTEFCSRYFKQFFELHPTDAIYYGIDGYDHLLNDYSDKSYQ
                     AEKLFVKESLRKLREIAPADLDNDEAIDHALLEGRLVIQSYEHAKEDYRLRQPDTYSP
                     TEAIYILTVRATKDFAGNLLSRLNRTPALVEQGITNLGRPAANPPKLWTENAIEGAKG
                     GIAFLDSLPEHPKVRSELKDTAALHRAIAKAKTAVHDFGDFLERHLLPRSNASYAVGE
                     EHFNLLLKKKHFLDHDAQSLLALGEALFARTKQELEALADELAPGQGVEAAARAIQEN
                     HPSSEQILSVYEKAMGAARAFVRRKQLVTFPAKEELHVVHTPVFRRHEIPFAAYLSPS
                     PKDPNQVGYYYVTPVTSDDLLREHNYVGLENTSVHESYPGHHLQFSIANCTPAAATLP
                     RLMNESSVFYEGWALYCEQLMQEQGFLNSQQHRFVMLKDRLWRALRIIIDVKTQTGKL
                     SYDEAADLMVRELHFPREQACGDLNWYSQSPSVPMGYALGWSIINRLREQEQKRLGDK
                     FTLCGFHDKLLSAGSIALPLVEKRYFMN"
     gene            complement(2701..3471)
                     /locus_tag="VMZ02_10265"
     CDS             complement(2701..3471)
                     /locus_tag="VMZ02_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72363.1"
                     /translation="MSQILRRIIELSCCAALLLFFSAPAPGQISGKIPADVIEHTASH
                     GSAWVVVGLDLPWKIESSLSQADLAAQRSVIATKQDDLLNQLAGRKFSVVRRYQEIPG
                     IALEVGADALAELARLSIVTNVLLDRPALPIQPQVDREVSPGTATGILSSEKVPSILF
                     DRATRDGTVLVLAGLRTPWQREDLLTDKLVDLQRHAIHGVQSYLLGELAGTHYKVLRL
                     YGKIPGIALRVGVDALVILERSTAITNVVADRPAQIAR"
     gene            3697..4818
                     /locus_tag="VMZ02_10270"
     CDS             3697..4818
                     /locus_tag="VMZ02_10270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72364.1"
                     /translation="MHRLIGLGLLHRITLAFVMLFCAAKARSAETPRPSQWEATIEAA
                     KKEGKVNIYLYRYGKVLDVFRSEYPEIRPYLLTGTGAQITTKILTERRAGRYLADVIG
                     LGSSNYRILHQQAKMLDPIQSALMLPEVLDTSRWYGGKHRYLDAENKFVFAYLANASS
                     GQLYYNTTQVNAREFTSYYDLFKPKWKGKIVMLDPRPRTEVGTTMQFFHYNPELGPGF
                     IRQLFGAKEVTFSRDSRQMIDWLGQGRFSICFGCSGALKAKNQGLPIEIFDTSLWKEG
                     ASFSVGGGTLSTPSQAPHPNAAKVFINWYLSRRGQSVLQKIGDPDEPPNSARIDIPKD
                     EIQVHNKLIEGRKYFDASRPEFEDVDSAFKIAVQAMEAR"
     gene            4877..>5582
                     /locus_tag="VMZ02_10275"
     CDS             4877..>5582
                     /locus_tag="VMZ02_10275"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HUR72365.1"
                     /translation="MELALLDYSIDQLEWGPQSVLRGRSLTIAQADIQHLLGDLCHGI
                     RVDYELVRPGESKRIVHVLDTVLPIAKLVGAGTAFPGFHDATNLVGTGATVRLKNLLV
                     TVAGRFPQFESLSPIEKPREGMIDMAGVGASYAYGSDRFHLVLSLTPDPSVSNAGFDQ
                     ALRNMALRCASFLAQVEKTNCEPAQQLIALPPVDVRLPKVVLIYQVQSQLCCARTFYY
                     AEEVSKTLPTFVHPTEF"
BASE COUNT         1181 a         1520 c         1627 g         1254 t
ORIGIN      
        1 tgctgcaaaa ataatgcggc aacgattttt attttgctcc gcgcgcgcat ctaattttca
       61 ataattagac tgcgtagaag cgttagattc tccatgtcct ctttcagttc cgggcccttc
      121 ggtgtttcca aacacatcgg caggccccaa aagcgttggt cgttcatcaa cagtcggaat
      181 ccttcgacgc cgatgaaccc tttaccgatg tgttcatggc ggtcgacccg agagtgaagt
      241 tctttttttg aatcgttcag atgaaatgcg gcgagcagct cgatgccgat ggtttggtcg
      301 aattctgcga aagtgcgctc atagccttct gcagttctga tgtcgtagcc ggcggcaaac
      361 gcgtgttccg tgtcgaaaca aacgcgcagg cggtcacact ctctggtggc gtcgatgatg
      421 tcgccgatct gttcgaagcg atagccgaga ttggagccct ggccggcggt ggtctccagc
      481 gtcactttca ccttgaagcc ggcgcatgct ttatggactt catcgagcga tttggcaatg
      541 gtctttatcc cttcgctttc gccggcgccg atatgcgcgc cgggatgggc gatcaaattc
      601 gaaacagcga gcacttcaca acgttccatt tcgtcgatga acgcgccaat ggaacgtttg
      661 cgcaatatcg gatcgggtga gcccaagttg agcagatagg aatcgtgggc aaccaccgcg
      721 ctgatgccgg tagatttccg attggcgtga aattgagcga tttcctcgtt gtccaacggt
      781 tttgacgccc actggcgcga cgacttggta aaaatctgaa tcgcctggca ggcgaccttc
      841 tggccttgga ggaaagcgtt gccgaccccg ccggcaatgg acatgtgggc gcctagtaac
      901 gggccgcgtg gttttgattt tggcttcata acgttttgaa cggcttgaac gatttaaact
      961 gcttgaacgg ttcgagacgt ggccctcggt tcaagccgtt caattcgttc aaaacgttga
     1021 aatcgtttcg gactaattca tgaaatatcg tttttccacc agcggtaaag caatgctgcc
     1081 ggcgctgaga agcttgtcgt gaaacccgca gagggtgaac ttgtcgccga ggcgtttttg
     1141 ttcttgttcg cgtaggcggt tgatgattga ccagcccaaa gcgtaaccca tcggcaccga
     1201 cggcgattgg ctgtaccagt tcaaatcgcc gcaggcctgc tcgcggggaa agtgcagttc
     1261 gcgcaccatc aagtccgcgg cttcgtcgta gctgagcttg ccggtttggg tcttcacgtc
     1321 gatgatgatg cgcaaggcgc gccacaggcg gtctttgagc atgacgaagc ggtgctgctg
     1381 cgagtttaaa aatccttgct cctgcatgag ctgctcgcag taaagcgccc agccttcata
     1441 gaacaccgaa gactcgttca tcaagcgcgg cagtgtcgcg gcagcggggg tgcagttggc
     1501 gatggagaat tgcaggtgat gtccgggata gctctcgtgc accgaagtat tctccagccc
     1561 aacgtagtta tgctcgcgca acaaatcatc gctggtcacc ggcgtgacat agtagtaacc
     1621 cacttgattc ggatccttgg gcgacggcga tagataagcg gcgaagggaa tctcgtgccg
     1681 gcgaaacacc ggcgtgtgga cgacgtggag ctcttccttg gcaggaaagg tcacgagctg
     1741 tttccggcgc acgaaagcgc gcgccgcgcc catggctttt tcgtagaccg ataagatttg
     1801 ttcgctcgag gggtggtttt cctggattgc tctcgctgcg gcttcgacgc cttgacccgg
     1861 ggccaattca tccgccaacg cttcgagttc ctgctttgtc ctggcgaaaa gagcctcgcc
     1921 caacgcgagc aggctttgcg cgtcgtggtc gagaaaatgt ttcttcttca aaagtagatt
     1981 gaaatgctct tcacccacgg cgtagctggc gttgctgcgc ggcaagagat ggcgctcaag
     2041 aaaatcgcca aagtcatgca ccgcagtctt ggctttggcg atggcgcggt gcaatgcggc
     2101 ggtgtctttc aattcgctcc gtactttcgg atgttccggc aggctgtcga gaaaggcgat
     2161 gccgcctttg gcgccctcga tggcgttctc ggtccacagt ttgggcggat tggcagccgg
     2221 gcggccgaga ttggtgatgc cctgctcgac cagcgccgga gtgcgattga gccggctcag
     2281 aagattgccg gcaaagtctt tggtcgctcg caccgtcagg atataaatcg cttcggtggg
     2341 cgaataagta tccggttgtc tgagacgata atcttccttg gcgtgttcat agctctggat
     2401 cacgagccgc ccttcgagga gcgcgtggtc gatggcttca tcgttatcca aatccgccgg
     2461 cgcaatttcg cgcagcttgc gcagcgattc tttgacaaaa agcttttcgg cctgataact
     2521 cttatccgag tagtcgttga gcagatggtc gtatccgtcg ataccgtaat agatggcatc
     2581 ggtggggtga agctcgaaaa attgtttgaa atagcggcta cagaattcgg taaacttgga
     2641 catgggcgag agatatcacc gaatcgcccg agtggcaatt ttgaccctgg cggaccgcga
     2701 ctagcgcgct atttgtgcgg gccggtccgc aacgacgttg gtgatcgctg ttgaacgctc
     2761 caggatcacc aaagcgtcca cgcctacgcg cagggcgatg ccggggattt tgccgtaaag
     2821 gcgcagcacc ttatagtgcg tcccggcgag ctcgccgagg agataactct ggacaccgtg
     2881 gatggcatgg cgttgcaagt ccaccagttt gtcggtcagt aaatcctcgc gttgccaggg
     2941 tgttcgcaat cccgccaaca cgagaaccgt gccgtcgcgg gtagcgcgat cgaaaaggat
     3001 cgatgggacc ttctcgctgc tcaaaatccc agtagcggtc ccgggcgaaa cctcgcgatc
     3061 gacctgcggc tgaataggaa gggctggacg atcgagcaaa acgttggtga cgatcgacaa
     3121 gcgcgccaac tcagccagcg catcggcgcc gacttccaac gcaatgccgg gaatttcctg
     3181 gtagcggcgc acaaccgaaa atttccggcc ggctaactga ttcaacaaat cgtcctgttt
     3241 tgttgcgatg acgctgcgct gcgcagccaa atcagcttgg ctgaggctcg actcgatttt
     3301 ccacggcaaa tccaagccca ccaccaccca agcgctgccg tgggaggcgg tgtgttcgat
     3361 aacgtcagcg gggattttac cagagatttg gcccggggcg ggcgccgaaa agaaaagcaa
     3421 cagtgccgcg cagcaactca gctcgattat tctccgaaga atttggctca tggcaatctc
     3481 catcggcctt ccgttagaca gaggatgcgc ggcgggcagt ttcaacccat gcaaagattc
     3541 tacacaggat ggataggctc tgtcgagggg cgattaaact tcgcggttcg ccgtgcgagt
     3601 agccgggacc gcggctagcg cagcgcgaaa attcaaattg acgatccgtc cagctgggat
     3661 atagtgacgc cacttctttc agggtggagg atagaagtgc atagactgat aggcctgggt
     3721 ttgctgcaca ggataacgtt ggctttcgtt atgttgttct gcgcggcaaa ggcgcgcagc
     3781 gcggagacgc cgcggccgtc ccagtgggaa gccaccatcg aggcggcgaa gaaggaaggc
     3841 aaggtcaata tctaccttta ccgctatggc aaggtgctcg acgttttccg tagcgagtac
     3901 ccggagattc ggccctattt gctcaccggt accggcgcgc agattacgac aaagattttg
     3961 accgagcgac gggccggacg atatctggcg gacgtcatag gattaggttc gtcgaattat
     4021 cggatactgc atcagcaggc gaaaatgctc gatcctattc aatcggcgtt gatgctgccc
     4081 gaggtgctcg acacatcgcg ctggtacggc ggtaagcatc gttatctcga tgccgagaac
     4141 aagtttgttt tcgcttatct cgccaacgcc agctcggggc agctctatta caacaccacc
     4201 caggtcaatg ccagggagtt cacctcctat tacgatctgt ttaagcccaa gtggaagggc
     4261 aagatcgtca tgctcgatcc gcggccgcgc acggaggtcg gcaccacgat gcagtttttt
     4321 cattataatc cagagctcgg gccgggattt attcgccagc tgttcggcgc caaagaagtg
     4381 acttttagcc gcgacagccg gcaaatgatc gactggctgg gacaaggaag gttctcgatc
     4441 tgcttcggtt gctccggcgc gctcaaggca aaaaatcagg gactgcccat cgagatcttc
     4501 gataccagtc tgtggaaaga gggcgcaagt ttttcagtcg gcggcggcac gttgtcgacg
     4561 ccgagccaag caccccatcc caacgccgcc aaggttttta tcaattggta tctgtcgcgc
     4621 cggggacaat cagtgctgca aaaaatcggc gatcccgacg agccgcccaa ctcggcgcgc
     4681 atcgatattc ccaaggacga gattcaggtg cacaacaaac ttatcgaagg ccgtaaatat
     4741 ttcgacgcca gccggccgga gttcgaagac gtcgatagcg cgttcaagat cgccgtccag
     4801 gcgatggagg cgcgatgaca gttcgccaaa tgcgccgatg tccgtaactc caatcaaaca
     4861 ggagaaagat taaccgatgg aactggcact gcttgactac tcgatcgacc agcttgagtg
     4921 gggcccgcag agcgtgctcc gtggccgatc gttgacaatt gcgcaagccg acattcaaca
     4981 cttgcttggc gatttatgcc acggcattcg cgtcgactac gaactcgtcc gtccgggcga
     5041 gtctaagcgc atcgtccatg ttctcgatac cgtgctgccg atcgccaagc ttgttggtgc
     5101 cggcaccgcc tttccaggct tccatgatgc gacaaacctc gtcggcaccg gggcaaccgt
     5161 gcgcctgaaa aatcttctgg tcaccgttgc cgggcgcttt ccgcagttcg aatcgctgtc
     5221 gccgatcgaa aagccgcgcg agggaatgat cgatatggcc ggtgttgggg cgtcctatgc
     5281 gtatgggtcg gatcgttttc atctcgtgct gtcgctcacg cccgacccgt cggtttccaa
     5341 tgccggtttc gaccaggcgt tgagaaacat ggcgctgcgc tgcgcgagtt ttctggccca
     5401 ggtggagaaa accaactgcg agccggcaca gcaactcatt gcactgccgc ccgtggatgt
     5461 ccgtttgccc aaagtggtgc tgatctatca ggtgcagagc cagctctgtt gcgcgcggac
     5521 cttctattac gctgaggaag tctcgaagac tttgccgacc ttcgtgcacc ccaccgaatt
     5581 ct
//
LOCUS       DATLCH010000347         6292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_11060708, whole genome shotgun sequence.
ACCESSION   DATLCH010000347 DATLCH010000000
VERSION     DATLCH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11060708"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /gene="ligA"
                     /locus_tag="VMZ02_10280"
     CDS             <1..1659
                     /gene="ligA"
                     /locus_tag="VMZ02_10280"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HUR72366.1"
                     /translation="IDGLAVELVYLDGKFAVGSTRGDGIHGEDITLNLKTIRSIPLTL
                     HSQGRKPPHRLEVRGEVFLGRAAFQKMNRERDEEGQPVFANPRNAAAGSLKQLDSTIT
                     AKRPLDIFCHGVGRIEGAAFASHWDFREHIQDWGLKPVPRGKLCHGIDEILRYREQME
                     TERDTLPYEIDGLVVKVNSTELQERLGQIARSPRWAIAYKFKPRQATTRILDIVAQVG
                     RTGTLTPVASLDPVPIGGVIVKSASLHNMDEIARKDIRIGDTIVIERAGDVIPYVVKV
                     VEDKRSGNEKPFTMPAHCPVCGAEVFREEGEAAYRCSGIACAAKLKESLKFFGSRGAM
                     DIEGLGEKLIDQLVDRRLVQDVADLYSLTKEQLANLARMADKSAQNLINALERSKSAT
                     LPRCLTALGIRHVGEATSKLLAEHFGTLEKIILATEEDLVEVREIGPEVAKSIARFFQ
                     QESNRQVIDKLRAAGLQFTVEKKKAGALTGATFVLTGGLEAMSRPEAQKRIEALGGRT
                     SSSVSRNTSYLVAGADPGSKLKKAQELGVKILREEELLELLRTG"
     gene            complement(1656..1979)
                     /locus_tag="VMZ02_10285"
     CDS             complement(1656..1979)
                     /locus_tag="VMZ02_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HUR72367.1"
                     /translation="MATKLYVGNLAYAVTDERLQQHFAQHGSVISARVITDKFSGRSK
                     GFGFVEMASEAEAAKALNALHDSEFEGHNIVVSEARPQTPRPSESGGYPAGGARPPRR
                     DRDRY"
     gene            2210..2500
                     /locus_tag="VMZ02_10290"
     CDS             2210..2500
                     /locus_tag="VMZ02_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HUR72368.1"
                     /translation="MTSAVEPRRVHLHISGRVQGVYYRASMLQKAQQLGLTGWVMNRP
                     DGSVESVAEGSKEKLDELIAWCHQGPEGARVVAVDAQWDSPENRFIGFAIRR"
     gene            complement(2497..3348)
                     /gene="rsmI"
                     /locus_tag="VMZ02_10295"
     CDS             complement(2497..3348)
                     /gene="rsmI"
                     /locus_tag="VMZ02_10295"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HUR72369.1"
                     /translation="MPGTLFIVPTPIGNLEDMTLRALRVLKEVELIAAEDTRHTQHLL
                     AHFGIHTALTSYHEHNERDKARTLVARLKSGASIALVSDAGTPAISDPGYRLVIDAIR
                     AGIQVIPLPGASALTTALSASGLPTDRFLFAGFLPAKTQERKAKLQGLRSESATLVFY
                     EAPHRLKESLADVLQVFGEREIAIARELTKVHEEFLRGKLSEIIGLLGGHEIKGEIVI
                     VIQGAAGEAQVSDAELQAMIGQLMADGMGMKEIAELLGERYGLPKKQVYKRALELKAP
                     KRPSDFG"
     gene            complement(3435..4256)
                     /locus_tag="VMZ02_10300"
     CDS             complement(3435..4256)
                     /locus_tag="VMZ02_10300"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HUR72370.1"
                     /translation="MVMIPTNTALLRQILVAFIRNEVLKTGLAHVVVGLSGGVDSALS
                     ALLAVEALGAKNVLGVLMPYKSSHPDSQTHAEMVVQRSGIESLLVEITPQIDAYFEKF
                     PGADARRRGNKMARERMTILYDHSMRWDALVLGTSNKTELLLGYGTLHGDMASALNPL
                     GDLYKTQVWALSEAVGVPQVIVAKDPSADLWIGQTDEDELGFSYREVDKLLYLMVDQR
                     YTKAELAAAGFPERFIDDIAARIMNSQFKRRLPVIAKVSQRTIDRDFRYSRDWGK"
     gene            complement(4311..5225)
                     /locus_tag="VMZ02_10305"
     CDS             complement(4311..5225)
                     /locus_tag="VMZ02_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HUR72371.1"
                     /translation="MSIRIAIGQVNPRLGDVQANLALYEEAIGRAVDDKAKLLLFPEL
                     SLTGYMLRDTVPAVALKLDSPEIKQLMALSRKIAFVAGLVEESADHRFFNSAIYFEDG
                     AVRHVHRKVYLPTYGMFDEQRYFAHGDRVRAFDTKFGRMALLICEDLWHPSTIYLAAL
                     DGALAVLCPSASPLRGIVDQQNQDDNARYWELLNRVYAETYSLFIAYGNRCGFEDGVG
                     FWGGSEIVDPFGERLGKARYYDEDFLTVDASFEAVRRKRTMSPLLRDEDLDLTINELM
                     RIRERPGGDTVQLSKGPKVQVRRTANKK"
     gene            5371..5586
                     /locus_tag="VMZ02_10310"
     CDS             5371..5586
                     /locus_tag="VMZ02_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258239546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UXX-star (seleno)protein family 1"
                     /protein_id="HUR72372.1"
                     /translation="MAENVTIYGQDLCAFTSAARADFSRRKVPFVYVNVLEDDAGLEQ
                     MMQLTNGRRKIPVIVEDGKVTVGFGGT"
     gene            5669..>6292
                     /locus_tag="VMZ02_10315"
     CDS             5669..>6292
                     /locus_tag="VMZ02_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72373.1"
                     /translation="MSGSIDQLIVDKVAVLYGRAAAVTSVTALPGDASSRRYYRVRLS
                     GVVPPSLMTMQLPVGSGLPISSEELAVFTEELKELPFLNVHRFLHGIGVRVPEVYGQW
                     ETEGLLFIEDLGDTPLWDRVQGLSENQVIAWYKKAIDELLRLQVVGTQKRDDACLAFQ
                     QRFDEKLYLWELEHFIEWGLEKRPGTRVTTKMLATLRKTFVSMAQRLD"
BASE COUNT         1410 a         1811 c         1761 g         1310 t
ORIGIN      
        1 atcgacggat tagcggtcga gttagtttat ctcgatggca aattcgctgt aggttcgact
       61 cgcggcgacg gcattcacgg cgaagacatc acgcttaatc taaaaactat ccgctcgatt
      121 ccattgacct tgcattccca gggccggaaa ccgccacacc ggctggaagt ccgtggcgag
      181 gttttcctcg ggcgtgcggc ctttcaaaaa atgaaccgcg agcgcgacga agagggccag
      241 ccggtatttg ccaatccacg caatgctgcc gcaggatcgc tcaaacagct cgactcgacg
      301 atcacagcaa aacggccgct cgatattttt tgccatggcg tcgggagaat cgaaggcgca
      361 gcattcgcaa gtcattggga ctttcgcgag cacatccagg actggggttt aaagccggta
      421 ccgcgcggca aactctgcca cggtatcgac gagatcttgc gctaccgcga gcagatggaa
      481 accgagcgcg acaccctgcc ctacgagatc gatggcctgg tcgtcaaagt gaatagcacg
      541 gagctgcaag aacggctggg acagatcgcc cgctcgccgc gctgggccat tgcctacaag
      601 tttaaaccgc gccaagcgac gacacgcatt ctcgacatag ttgcccaggt tggccgcacc
      661 gggacgctga caccggtggc aagccttgat ccggtcccca tcggcggtgt tatcgtcaaa
      721 agtgcctcac tgcacaacat ggacgagatc gcccgcaaag atatccgcat cggcgacacc
      781 atcgtcatcg agcgcgccgg cgacgttatt ccctacgtcg tcaaagtcgt agaagacaaa
      841 cgcagcggca acgaaaagcc tttcaccatg ccggcgcact gccctgtgtg cggcgcggaa
      901 gtgtttcgtg aagaaggcga agccgcctac cgctgcagcg gcatcgcctg cgccgccaag
      961 ttgaaagaga gtctgaagtt tttcggttct cgtggcgcca tggatatcga agggctcggt
     1021 gaaaaactca tagatcaatt ggtagaccgc cgtctggtgc aggacgtcgc cgatctttat
     1081 agcctgacga aagaacagct cgccaatttg gcacgcatgg cggacaagtc ggcgcagaat
     1141 ttaatcaatg cattggaacg cagcaagagt gcgacactgc cgcgctgttt gaccgcactg
     1201 gggattcgtc atgttggcga ggcaacgtcc aaactgctgg ccgagcactt cggcacactc
     1261 gaaaaaatta tcttagcgac cgaagaagac ctcgtcgaag ttcgcgaaat aggccccgaa
     1321 gtggccaaaa gcatcgcacg cttttttcaa caagaaagta atcggcaggt gatcgacaag
     1381 cttagagcag ccggactgca attcacagtt gaaaaaaaga aagccggggc tctgaccggc
     1441 gcgacgttcg tgcttaccgg cggactcgaa gcgatgtcac ggccggaagc gcagaagcgc
     1501 atcgaagcgc tcggtgggcg gacgtcgtca agtgtcagcc gcaacaccag ctacctcgtc
     1561 gccggtgcgg atcccggctc caagctcaag aaagcccagg agctgggtgt gaaaattttg
     1621 cgcgaagaag agttgctcga gctgctgcgc accggctagt agcgatcacg atcccgtcgc
     1681 ggcggccgcg cgccaccggc ggggtatccg ccgctctcgc tcgggcgcgg cgtctggggg
     1741 cgcgcttcgc tgacgacaat gttatggccc tcgaattcgc tgtcgtgcag ggcattcagc
     1801 gctttcgccg cttcggcttc ggacgccatc tcgacaaacc caaagccttt ggaccggccg
     1861 ctgaatttgt cggtgataac ccgcgcacta atcaccgacc catgctgggc aaagtgctgt
     1921 tgaagccgct cgtcagtcac cgcgtaggcg agattcccga catatagctt cgtcgccatc
     1981 tgggcctcct agtcgatcga gttctgggaa agcccgcatg aagccagtct ccgtgcacat
     2041 tcgttaacgg gctctgccaa aaaacgccga taggctacaa aaatcgcgac tcgcttgtca
     2101 aacttttaat atcaaccaac taggtgaatg tgacaattgc tcgaccttcc ggtaggtatc
     2161 cccttgttgc cgcagacacc catctgggtt aaaaaattgc gcgagactta tgacaagtgc
     2221 ggtagaacca aggcgcgtcc acctgcacat ctccggccgt gtccagggag tgtattaccg
     2281 cgcttcaatg ttacagaagg cgcagcagtt aggtctcacc ggctgggtca tgaatcgtcc
     2341 cgacgggtcg gtcgaatcgg tggcagaagg cagcaaagag aaattggatg aactcatcgc
     2401 ctggtgtcat caaggaccgg aaggcgcccg ggtcgtcgct gtcgatgcgc aatgggatag
     2461 tcctgagaac cgatttatcg gcttcgcaat taggcgttag ccaaagtcac tgggccgctt
     2521 tggcgctttg agttcgaggg ctcttttata aacttgtttc ttcggtaatc cgtagcgctc
     2581 gcctaaaagt tcagcgattt ccttcatccc cattccatcg gccattaact gaccgatcat
     2641 cgcttgcaat tctgcgtcgg acacctgcgc ctcgcccgcc gcgccctgaa taactatgac
     2701 gatttcgcct ttgatctcat ggccgccgag cagaccaata atttcggaca attttccgcg
     2761 cagaaactct tcgtgaactt tggtcaactc acgggcaatg gcaatctcgc gctcgccaaa
     2821 gacctgcagt acgtcggcaa gagactcctt cagccggtgc ggcgcctcgt agaacaccaa
     2881 cgtcgcactt tcactgcgca gtccttgcag tttagccttg cgctcttgcg ttttggccgg
     2941 tagaaaaccg gcaaataaaa atcgatcggt aggtaagccg ctggcgctca gtgccgtggt
     3001 caacgccgat gcgccgggca gcggaattac ttgaatgcca gcacggatag cgtcgatgac
     3061 aagtcgataa ccagggtcag aaatcgccgg tgtgccggcg tcggacacca gtgcgatact
     3121 ggcgccgctt ttgaggcgcg cgaccagcgt gcgtgctttg tcgcgctcat tgtgttcgtg
     3181 atagctggta agcgccgtgt ggatgccgaa gtgcgcgagc aaatgctggg tatgacgggt
     3241 atcctccgcc gcaatcagtt cgacttcctt cagaacccgt aacgcgcgca gcgtcatgtc
     3301 ttcgagattg ccgatcggcg ttggcacgat gaagagcgtg ccgggcattc aatgcctccg
     3361 tcgcggttcg aggacaaata atcgaggttt gaggacggcg cgatcttcgc tactcggttc
     3421 tccagccccg accatcattt gccccaatcc ctggagtaac ggaagtcgcg atcgatggtg
     3481 cgttgggaga ctttcgcgat cacgggcaag cgccgtttga attgggaatt catgatgcgc
     3541 gcggcgatgt cgtcgataaa gcgctcggga aagccagcag cggccaactc ggctttggtg
     3601 taacgctggt cgaccattaa atagagcagt ttatcgactt cccgatagct aaagcccaac
     3661 tcgtcttcgt cggtctggcc gatccagaga tcggccgacg ggtccttggc gacgatcacc
     3721 tgcggcacac cgaccgcttc ggacagcgcc caaacttggg ttttatagag atcacccagc
     3781 ggattgagcg ccgatgccat gtcaccgtgg agcgtaccat atcctaagag gagctcggtc
     3841 ttattgctcg tgccaagtac tagcgcatcc cagcgcatcg aatgatcgta caggatcgtc
     3901 atgcgttcgc gcgccatctt gttgccgcgc cggcgcgcgt cggcgccggg aaacttttcg
     3961 aaataggcgt cgatctgcgg cgtgatttcc accaacagcg attcgatgcc gctcctttgc
     4021 acgaccatct cggcatgggt ctggctgtcc gggtgggaac tcttataggg catcaacacg
     4081 cccaaaacat ttttcgcgcc caaagcctct actgctaaca gcgcactcaa tgccgaatcg
     4141 acgccgcccg agagccccac cactacatgc gcgagcccgg tcttgaggac ttcgttgcga
     4201 ataaatgcaa cgagaatctg gcgcagcaaa gcagtgttgg tagggatcat gaccatggtg
     4261 aggcgaccgc tggaattttt ctcgtggaca ccggaaagct tgaccgcagg tcatttctta
     4321 ttcgcggttc tccgcacttg aacctttgga cccttcgaaa gttgcacagt atccccgcca
     4381 ggccgctcgc gaatacgcat tagttcgttg atcgtcaagt ccaaatcctc gtcgcgcagc
     4441 agcggtgaca tggtgcgctt gcgccgcacc gcctcgaagc tcgcatcgac tgtgaggaaa
     4501 tcctcgtcat agtagcgcgc cttccccagg cgctcgccaa agggatcgac gatctcggac
     4561 ccgccccaaa agccgacacc gtcttcgaaa ccgcaccggt tcccataggc tatgaaaagg
     4621 ctataggttt cggcatagac acgattgagc agctcccagt agcgggcgtt gtcatcctgg
     4681 ttctgctgat cgacaattcc gcgtagcgga ctcgccgatg ggcaaagcac cgccagcgca
     4741 ccatccaaag cggccagata aatcgtcgat gggtgccaga gatcttcaca gatcaacagc
     4801 gccatgcggc cgaatttagt gtcgaacgcc cgcacacggt cgccgtgcgc gaaatagcgc
     4861 tgttcgtcga acatgccgta ggtgggcaga tagactttac ggtgcacatg gcgcaccgcg
     4921 ccatcttcga aatagatcgc cgagttgaag aagcgatgat cggcgctctc ttcgaccagc
     4981 ccggcaacga aagcgatttt tcggctgagc gccataagct gcttgatctc gggcgaatcc
     5041 agtttcagcg ctaccgccgg cacggtatcg cgcagcatat agccggttaa actcaattcg
     5101 gggaataaca ataatttggc tttgtcatcg acagcgcggc cgatggcttc ttcatacaga
     5161 gccaggttgg cctggacgtc gccaagtcgc ggattgacct ggccgatagc gatgcggatg
     5221 gacacgcgaa actctccgtc tgcgagcacg ctggaaataa attcttacga aactaacata
     5281 cgccgaacca cacttgcaat ttaccccagc gacgcttaac ttggtcaagt cggcgagtcc
     5341 acgctttgcc gatattcaga gcgcaatccc atggcagaaa acgtgacgat ttacggccaa
     5401 gacttgtgcg cctttacttc ggcagcccgt gcggacttct cccggcgcaa agtgcccttc
     5461 gtatatgtga atgtcttgga ggatgacgct ggtctcgagc aaatgatgca gctcaccaac
     5521 ggccggcgca agatcccggt aattgtcgaa gatggtaagg taaccgtcgg attcggcggc
     5581 acctgagagg tgtgaacggc cgacggtgtc ggccatcgct cgtagttcac cccggccaag
     5641 taatagctag cgaaactcta attccccctt gtctggatcc atcgatcaac tgattgtcga
     5701 taaagttgcc gtgctctacg gccgcgccgc cgccgtgacc agcgtcacgg ctcttccagg
     5761 cgacgcctcg agccggcgtt attatcgcgt gcgactgagc ggcgtcgtac cgccttcctt
     5821 gatgacaatg caactaccgg tcggcagtgg cctacccatc tcgagcgagg agttggcagt
     5881 cttcactgaa gaactcaaag aactgccgtt tttgaatgtg caccggtttc tccatgggat
     5941 cggcgttaga gtgcccgagg tatacggtca gtgggaaacc gagggcctcc tgtttatcga
     6001 agatttgggc gacactccac tatgggatcg cgtccagggt ttatccgaaa atcaagtaat
     6061 cgcctggtac aaaaaagcga tcgacgaatt gttgcgctta caagtcgtcg gaacgcagaa
     6121 gcgtgacgat gcctgcctcg ccttccagca acgcttcgac gaaaaactct acctgtggga
     6181 actcgagcat tttattgaat ggggtctgga aaagcggcct ggcacccggg tcacaacgaa
     6241 aatgcttgcg acactgcgta aaacttttgt ttccatggcg caaaggctcg at
//
LOCUS       DATLCH010000348         3872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8849067, whole genome shotgun sequence.
ACCESSION   DATLCH010000348 DATLCH010000000
VERSION     DATLCH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8849067"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VMZ02_10320"
     CDS             <1..677
                     /locus_tag="VMZ02_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR72374.1"
                     /translation="GQVMEALIGIDAVIHLAAIHNPNIATAPLVYQTNVVGTFNVHHA
                     AYRLGVKRVVSASSNAIVGWSYSERFEPDYLPIDEDHALRPEDVYGLSKQIGETIAHS
                     YARKDVETIILRPSGVVTPEELIELKKAGGRTATSFQAFSYIDARDLALAFRLSVEKS
                     LPSGTTLFVVADDSNVAEPLCDLLPRLRPSIGDKARSLIGTKGAYSNARAKALLGWQP
                     QHSWRT"
     gene            688..1797
                     /locus_tag="VMZ02_10325"
     CDS             688..1797
                     /locus_tag="VMZ02_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72375.1"
                     /translation="MKRRATLLVLVVIVACVVPPVETAEISTKHTNEWAGLIKGAVSE
                     GQVTVYATNGFEPLFDAFQKRFPEIKFNGVIGRGNQLAPKILAERRAGKFLADVYLGG
                     IGTPYSVFYKAGMLEPIEPLLTVAEVTDKSKWFQGQFHYADNDGRYIFIFEFALRADI
                     AYNRQQVRPDEIASYWNLVEPKWRGKIVAIEPHQTGVATGSSLSFFYYHPELGADFLR
                     RLFGQMELTFSRDTRQMLDWLAVGKFSLALFASDADVAVRQGLPIGRFAPGSFKEGAY
                     GRPQRGTVSVFNRAPHLQAAKLFVNWLLSREGQHTFQNAFADDYSLSVREDLSKDHIP
                     AAYRLGRSEKFFPAYRPEYIDTKPALKVIDEALKK"
     gene            2112..3101
                     /locus_tag="VMZ02_10330"
     CDS             2112..3101
                     /locus_tag="VMZ02_10330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72376.1"
                     /translation="MRRIFHALLLMAVAVLLLNGPVLAQTIKVPYVSISGFQAPLYLG
                     ERAGLFKKNQLDAQLIYMPGGSLIVQTLLSGDVGVASLAPPSAVSAWAKGAELAIVAG
                     GIERALNVLMVNPKIKRVDELKGKRVAISRFGSLSDVSLRDALAYHKLKNQDIVIAQM
                     GGLGERMVALTSGIVDGAILNVDQVYQAEKLGFQVLIDMRKLPLNYSTQGIVVSREFL
                     RTQRGVVKRFLRVYIEGIKLFKTDKELSIDTLGRYIKTNDREVLAKTYDFYREAWESL
                     PTVRREGIQQALDSIHEGKNPKLNLDSLIDNSLIQELEREGFLRELYPEGLKR"
     gene            3302..>3872
                     /locus_tag="VMZ02_10335"
     CDS             3302..>3872
                     /locus_tag="VMZ02_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR72377.1"
                     /translation="MLKPIGLIEGHYECRSLDETLPVFTDLLAMEVVERNAKQATLKH
                     PNTDWRLIVHESGANAPEKTFDNHYGFRVASHTEVEAAWQYLEANKDKYRLSKITKPQ
                     SAHFAYSVYFHEPGGNHLEIEYYNPGSALHGRGHTAGHWDKPLTAERFPDRGYIPQAL
                     THGTLQCDDLGKTRAFLIEVLGLDDAGGMS"
BASE COUNT          900 a         1145 c         1012 g          815 t
ORIGIN      
        1 ttggtcaggt tatggaagcg ctgatcggca tcgacgcggt cattcacctg gcggcgattc
       61 acaatcccaa catcgccact gcaccgctgg tctatcaaac caatgtggtc ggcactttca
      121 atgtacacca tgccgcgtat cgtctcggcg tcaaacgggt cgtcagcgcc agcagcaacg
      181 ccatcgtcgg gtggagctac agcgagcgct tcgaacccga ctacctgccg atcgacgaag
      241 atcatgcgct gcgtccggaa gatgtttacg gcttgtcgaa acagatcggc gaaaccattg
      301 cgcatagcta cgcccgcaaa gatgtggaaa caataattct ccgcccgagc ggcgtggtca
      361 cgccggaaga actgatcgaa ttaaaaaaag ccggtggacg cacagccaca tcgtttcagg
      421 ctttcagtta tatagacgcc cgcgacctcg ccttagcgtt ccgactatcc gtcgaaaaat
      481 cattaccaag cggcacgacc ttgttcgtcg tcgccgacga tagcaacgtc gccgaaccgc
      541 tgtgcgattt attgccacgg ctgcgtccat cgattggcga taaagctcgg tcgcttatcg
      601 gcacgaaagg cgcttattcc aacgcccgcg ccaaagcgct gctcggttgg caaccgcaac
      661 attcctggcg cacttaaaga attaataatg aaacgtcgtg ccacactgct ggtcctcgta
      721 gtgatagttg cttgcgtcgt cccgccggtc gagacggcag aaatttccac caagcataca
      781 aacgaatggg ccggtttgat caaaggcgcg gtcagtgaag ggcaagtcac cgtctatgcc
      841 accaacggct tcgagcctct cttcgatgct tttcaaaaac gctttcccga gatcaaattc
      901 aacggagtca tcggccgcgg caatcaattg gcgccaaaga ttctcgctga gcggcgcgcc
      961 ggcaaatttc tcgccgatgt ttacctcggc ggcatcggca cgccgtactc ggttttttac
     1021 aaagccggca tgctcgagcc gattgagccg ctactcacgg tcgccgaagt gaccgacaaa
     1081 tcgaaatggt tccaaggcca gtttcactat gccgataatg acggccgcta tatctttatt
     1141 tttgaattcg ctctccgcgc cgacattgct tataaccgcc aacaagtccg ccccgacgaa
     1201 attgcttcgt actggaacct cgtcgagccc aagtggcgcg gcaagatcgt cgccatcgag
     1261 ccgcatcaga ccggcgtcgc caccggcagc agcttgagct tcttttacta ccatcccgag
     1321 ctcggcgcgg attttttacg ccggctattc gggcagatgg agctgacgtt ttcccgcgac
     1381 acccgccaaa tgctcgattg gctcgctgtc ggtaagtttt ctctagcgct gttcgcgagc
     1441 gatgccgacg tcgcggtccg tcagggattg ccaattggcc ggttcgctcc tggaagcttt
     1501 aaggaaggag cctacggccg gccgcaacgc ggcacggtgt cggtttttaa ccgggcgccc
     1561 catctgcaag cggccaaact gttcgtcaat tggttgttgt cgcgggaagg ccagcatact
     1621 tttcaaaatg cctttgccga cgactactcg ctttcagtcc gcgaggacct gtctaaagat
     1681 cacattcccg ccgcttatcg tctcggccgg agcgaaaaat tttttcctgc ttaccggccg
     1741 gaatacattg ataccaaacc cgctctcaaa gtaatcgacg aagctctgaa gaaataattc
     1801 taaccggccc gggaacggga agaacgagcg gactctcagc taatttaatc gccgccattt
     1861 cacgcaccgt cggcgccgca ctttgcaaac acgcggtatg ctgcgatcgg atttattcgt
     1921 ttgacgacgc acacgctccc ggtagaatga ccgccgcacc agccagcgcg ttgcaagaag
     1981 cgttaagcat gaacaaaatg tcaaatcgcg ttcccgttgt acttgcaccg atcgcggcgc
     2041 cttcttgctc gcggcaggat tggctcacgg cgcctaaaac ttcggaatgc ttcgaccaaa
     2101 ggagtcaaat catgcgccga atttttcatg ctcttttgtt gatggccgtc gccgtactgc
     2161 tgcttaacgg ccctgtcctc gcccagacca tcaaagttcc ttacgtttcg atttcaggct
     2221 ttcaagcgcc gctctatctc ggtgaacgcg ccggcctatt caagaaaaac cagttggacg
     2281 cgcagctgat ttacatgccc ggcggctcgt tgatcgtgca aactctgctc tcgggcgatg
     2341 tcggcgtcgc cagcctggcg ccgccctcgg cggtgagcgc ttgggccaag ggcgccgaac
     2401 tggccatcgt cgccggcggc atagagcggg cgctgaacgt gctcatggtc aatccgaaga
     2461 ttaaaagagt cgacgagctc aagggcaagc gtgtcgcgat cagccgcttc ggctcgctct
     2521 ccgatgtctc gctgcgcgac gccctggcgt atcacaaact caaaaatcaa gatatcgtga
     2581 tcgcgcaaat gggcggtctc ggcgaacgca tggtcgcgct tacctcgggt attgtcgatg
     2641 gagcgatctt gaacgtcgat caagtttatc aagcagagaa actcggcttt caggtgctca
     2701 tcgacatgcg caagctgccg ctcaactatt cgacccaagg catcgtcgtc agccgcgaat
     2761 tcctgcgcac acagcgcggc gtggtgaaac gatttttgag agtctatatc gagggcatca
     2821 aactttttaa gaccgacaag gagttgtcga tcgacacctt gggtcgctac atcaaaacca
     2881 acgaccgaga agtactggca aaaacctatg acttttaccg cgaagcatgg gagagcttgc
     2941 cgacggtcag acgcgaaggt atccagcaag ccttggacag catccacgag ggcaagaatc
     3001 ccaagctgaa tctcgatagc ttgatcgaca attcgctcat ccaggagctg gaaagagaag
     3061 gcttcttaag agagctttac cccgagggac tcaagcgttg agcatcgcat tggggcgtaa
     3121 ctaatcgcgc aacattccgc tccgcgatgt ccgcgcaagc cggtatccgg gccaggagaa
     3181 cgcaaatgtc aagcagcttt ccgcgtccgc gggaagcatg caacgatgac ttcggcgttt
     3241 gacacctcgc ctacccgcta gttagataac aacgagtgaa ctcatcgact gaggtaacaa
     3301 catgctcaaa cccatcggcc tgatcgaggg ccactacgaa tgccgctcgt tggacgaaac
     3361 cctgccggtc tttaccgatc tgttggcgat ggaagtcgtc gaacgcaatg ccaaacaagc
     3421 caccctcaaa catcccaaca ccgactggcg tctgatcgtt catgaaagcg gtgccaacgc
     3481 gccggagaaa accttcgaca accattacgg ttttcgcgtc gccagccaca ccgaagtcga
     3541 agctgcgtgg caatatctcg aagcgaataa agataaatat cgcttgagca aaatcactaa
     3601 accacagagc gcccacttcg cctactcggt ctacttccac gagcccggcg gcaatcattt
     3661 ggagatcgaa tattataatc ccggcagtgc cctacacggc cgcggtcaca ccgccggtca
     3721 ctgggacaag ccgctcacgg cagaacgctt tcccgaccgc ggctatatac ctcaggcgtt
     3781 aactcacggg accctgcagt gcgatgatct gggaaaaacc agagcgtttc tcattgaagt
     3841 gctcggcctc gatgatgcag gaggcatgag cg
//
LOCUS       DATLCH010000349         4472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_11081468, whole genome shotgun sequence.
ACCESSION   DATLCH010000349 DATLCH010000000
VERSION     DATLCH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11081468"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..555
                     /locus_tag="VMZ02_10340"
     CDS             145..555
                     /locus_tag="VMZ02_10340"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR72378.1"
                     /translation="MPIEPYLFFNGRCEEAIEFYKKALGAELTMLMRYKESPEPPPPG
                     MVPAGWDNKIMHASLRVGNANLMASDGCDQELNFQGFSLSLTATDEADANRKFTALAD
                     GGQVRMPLSKTFWSPCFGMVADRFGVGWMVTVAF"
     gene            583..954
                     /locus_tag="VMZ02_10345"
     CDS             583..954
                     /locus_tag="VMZ02_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUR72379.1"
                     /translation="MKFMLLVHHDEAAFELIGQEKQQQMLAGSVALTHQLHTHGQYLS
                     ASPLQPAATSVIVRVRESKTIITDGPFIETREQLAGYFLIDARDLNEATQIATRVPGA
                     RIGTVEVRPLREISGLPGESC"
     gene            948..1151
                     /locus_tag="VMZ02_10350"
     CDS             948..1151
                     /locus_tag="VMZ02_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72380.1"
                     /translation="MLRRRGLCFDNNAEEAVNHHRLSWQVVTSALIEMLQDENERKSV
                     RVKPALMQMSTLDIATLKQAFDR"
     gene            1176..1535
                     /locus_tag="VMZ02_10355"
     CDS             1176..1535
                     /locus_tag="VMZ02_10355"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HUR72381.1"
                     /translation="MAYVDGYILPVPKRKIAAYRRMAKMAGKVWRDHGALEYVECIAD
                     DVKSGKHTSFPQSVKLKPGETVIFAYIVYKSRAQRDRVNAKVMKDPRLAQSMDPKAMP
                     FDAKRMFWGGFKVLIRR"
     gene            1549..2013
                     /locus_tag="VMZ02_10360"
     CDS             1549..2013
                     /locus_tag="VMZ02_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR72382.1"
                     /translation="MQRKVKPIPPGYHTVTPYLCVNGAASAILFYKKAFAAKEIMRMP
                     GPGHSIGHAEIQIEDSRIMLADEHPDMNFRGPHSFGGTAVHIHLYVQDVDRVVKKALS
                     AGAKLLRPVADQFYGDRSGSLEDPFGHVWHVASHKENLSTQELKKRAAAMAA"
     gene            2040..2399
                     /locus_tag="VMZ02_10365"
     CDS             2040..2399
                     /locus_tag="VMZ02_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUR72383.1"
                     /translation="MKYMLLIYLDEQNPLSEAERQECYAESTQLAQELHSNGKYLSAN
                     PLHPTSMATSVRVRNGKRLVTDGPFAETREQLGGYFLIDAEDLDDALGVAARIPMVRK
                     GTVEVRPVIDIPGLPTG"
     gene            2459..3646
                     /locus_tag="VMZ02_10370"
     CDS             2459..3646
                     /locus_tag="VMZ02_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72384.1"
                     /translation="MSGVRVLVGTRKGAFILSSDGTRKNWDISGPHFAGWEIYHLKGS
                     SVDPNRIYASQSSSWFGQIIQRSDDGGKTWHQPGTPAGEPTTAPDGSPKGESNNFAYD
                     TSAETGKQLTTHQWYDGTPHPWEFKRVWHLEPSLSDPDVVYAGVEDAALFRTSDGGTS
                     WQELAGLRGHGTGAQWQPGAGGLCLHTIILDPRNPERIYIAISAAGAFRTDDAGKTWK
                     PINRGLHSQYIPDPTAEIGHCVHHVAMHRARPGVLFMQKHWDVMRSNDGGDSWHEISG
                     NLPSDFGFVIDVHAHEPDTLYVVPIKSDSEHFPPDGKLRVFRSRNGGTEWEALTNGLP
                     QSNCYVNVLRDAMAVDSLDKCGVYFGTTGGQVYGSADAGDSWQPIVRDLPAVVSVEVQ
                     TLR"
     gene            3643..3939
                     /locus_tag="VMZ02_10375"
     CDS             3643..3939
                     /locus_tag="VMZ02_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HUR72385.1"
                     /translation="MIRVTLPAHLRKLAQVDDEIHLEIAGQITQRTVLDALEGRFPML
                     CGTIRDHATQKRRAFVRFFACGQDLSHESPDAMLPAAIASGAEPFMIIGAIAGG"
     gene            4021..4410
                     /locus_tag="VMZ02_10380"
     CDS             4021..4410
                     /locus_tag="VMZ02_10380"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUR72386.1"
                     /translation="MIKKVFIALAALIIGFVVIVALQPSEFRVSRSINIAAPAQAVFE
                     QVNDFHKWDAWSPWAKRDPNMRQTYEEAPAGVGASYSWSGNNEVGEGRMTLTETRPNE
                     MVRVKLEFLRPFAATPYHRVQFSAQRQ"
BASE COUNT         1052 a         1243 c         1257 g          920 t
ORIGIN      
        1 ggccctgctc gcaaaattat gaagtgagat aaaaattata ccgccgtcgt cgatttcggc
       61 cggtgccgaa cgactactgg ttggacagat tatttaatgc aaaatcgatc aattaaaaac
      121 gtcacattaa cgggaggaaa aattatgcct atcgaaccct atttgttttt caatggacga
      181 tgcgaagagg caatcgagtt ttacaagaag gctttgggcg ccgagctgac aatgctgatg
      241 cgttacaaag aaagcccgga accgccgccg cccggcatgg tcccggcggg ctgggacaat
      301 aagatcatgc acgccagttt gcgcgtcggc aacgccaatc taatggcctc ggacggttgc
      361 gatcaggagc ttaatttcca ggggttttca ttgtcattaa cagctacgga tgaagccgac
      421 gcgaaccgca agttcactgc gctcgcggac ggcgggcagg tgcggatgcc actttccaaa
      481 acgttctggt cgccttgctt cggcatggtc gccgaccgat tcggcgttgg ctggatggtc
      541 acggtggcgt tctgagtagc tcgccgaggc tggagatgac ttatgaaatt tatgttgctg
      601 gttcatcacg acgaagccgc tttcgaattg atcggccagg aaaaacaaca gcagatgctt
      661 gccggatcgg tggcgctcac gcaccagctt cacacccatg gccaatacct aagcgcatca
      721 ccgttacagc ccgcggcgac gtcagtcatc gttcgggtgc gagagagcaa gacgatcatc
      781 acggacggac cctttatcga gacgcgcgaa cagctcgccg gttacttttt gatcgatgcc
      841 cgggatctca atgaagcgac gcaaatcgcc acccgcgtgc caggagcacg catcggaacc
      901 gtcgaagtgc ggccactacg ggaaatctcc gggctgccgg gagaatcatg ctgaggagac
      961 gcggcctgtg tttcgacaac aacgctgaag aggcggtaaa ccaccaccgg ctctcctggc
     1021 aagtcgtcac ctccgctctg attgaaatgt tgcaagacga gaatgaaaga aaatcagtaa
     1081 gagtaaaacc ggcgctgatg cagatgagca ccttagatat tgcaacattg aagcaggctt
     1141 ttgaccgcta attatcaact atttggagag ttcccatggc ttatgtcgat ggatatattt
     1201 tgccggtccc gaagcgaaaa atcgcagctt accggcgcat ggcaaagatg gcgggtaaag
     1261 tctggcgcga tcatggtgct ctcgaatacg tcgagtgcat cgccgacgat gtcaaatcgg
     1321 gcaagcacac ttcgtttccg cagagcgtca agttaaaacc tggcgaaacc gtgatcttcg
     1381 cttatatcgt ctacaaatcc cgtgcgcagc gcgaccgcgt caacgctaaa gtcatgaaag
     1441 atccgcgttt ggcgcagtcg atggatccca aagccatgcc gtttgacgcc aagcgcatgt
     1501 tttggggcgg tttcaaggtt ttgatccgcc gctaggaagg agtcgtctat gcaaagaaaa
     1561 gtaaaaccga ttccgccggg atatcacacg gtcacccctt atttgtgtgt gaacggcgcg
     1621 gcgagcgcca tcctgtttta caagaaagcg ttcgccgcta aggagatcat gcggatgccc
     1681 gggccaggcc attccatcgg ccatgcggag atccagatcg aggactcccg aattatgctc
     1741 gccgacgagc atcccgacat gaattttcgc gggccccatt cattcggcgg cactgcggtg
     1801 cacattcatc tctatgtgca ggatgtcgac cgcgtggtaa agaaggcgct cagcgccggc
     1861 gccaaactgt tgcgacccgt ggccgaccaa ttttacggtg accgcagcgg ctcgctggaa
     1921 gacccgttcg gacacgtctg gcatgtcgcc agccacaaag aaaatctttc gacccaggaa
     1981 ttgaaaaagc gcgcagccgc catggccgcg taggctgaaa aaaattgtat ggagtgaaga
     2041 tgaaatacat gttgctgatt tacctcgacg aacaaaatcc gttgagcgaa gccgaacgcc
     2101 aggagtgcta tgctgagtcg acacaactcg cgcaagagct tcattcgaat ggaaagtatc
     2161 tgagcgccaa tccgttgcac ccaacctcga tggcgaccag cgtccgcgtg cgcaatggca
     2221 aacgcttggt gaccgatgga cccttcgccg agacgcggga gcaattgggc ggatactttt
     2281 taatcgatgc cgaggatctc gacgacgcgc tcggcgtcgc cgcccgtatt ccgatggtgc
     2341 gcaaaggcac cgttgaggtt cggcccgtga ttgacattcc gggtctgccg accggctaac
     2401 aaacttaacg acgattgtcg gcaaagcaca taaaccttaa tagcaagagg agtaaatcat
     2461 gagcggagta cgagtattgg taggcacacg caagggcgca ttcattctgt cctccgacgg
     2521 tacgcgcaag aactgggaca tcagcggacc ccacttcgcc gggtgggaga tttatcatct
     2581 gaaaggatcc agcgtcgatc cgaatcggat ctatgcttca cagtcgagca gctggtttgg
     2641 ccaaatcatt caaagatcgg acgacggcgg caagacctgg caccagccgg gcactcctgc
     2701 cggcgaaccg actacggctc cggacggctc acccaagggc gaaagcaaca atttcgcgta
     2761 cgacacttct gctgagaccg gcaagcagct gacgacgcat caatggtacg atggcacgcc
     2821 gcacccttgg gagtttaaaa gagtatggca tctcgaaccc tccctcagcg atcccgatgt
     2881 cgtgtacgcg ggagtcgagg atgccgcgtt gttccgcacc tcagatggcg gtacatcctg
     2941 gcaggaattg gccggcctgc gcggccacgg caccggagct caatggcagc ccggcgccgg
     3001 cgggctatgc ctgcatacga ttatcctgga tccccgtaac ccagaacgta tttacatcgc
     3061 gatctcggcg gctggcgcgt ttcgaactga cgacgctggc aaaacgtgga agccgatcaa
     3121 tcgcggactg cactcgcaat atattcccga tccgacggcg gagatcggcc actgcgtcca
     3181 tcacgttgcc atgcaccggg cgcgtcccgg cgtgcttttt atgcaaaagc attgggacgt
     3241 catgcgcagc aacgatggcg gcgattcctg gcatgagatc agcggcaact taccgagtga
     3301 tttcggcttc gtgatcgatg tccacgccca cgaacccgac accctctacg tggtgcccat
     3361 caaaagcgac tccgaacatt ttccgcccga cggaaagttg cgtgtattcc gcagccgcaa
     3421 tggcggcacg gaatgggaag ctcttaccaa cggcctgccg caaagtaact gttacgtgaa
     3481 tgtcttgcgc gacgccatgg ccgtcgattc actcgacaaa tgcggggtgt acttcggaac
     3541 cacaggagga caggtctatg ggtccgctga cgccggtgac agttggcagc cgatagtccg
     3601 cgaccttccg gcggtggtct cagtggaagt tcaaacactg cgatgatccg cgtcacacta
     3661 ccggcgcact tgcgtaaact ggcacaagtc gatgacgaaa tacatctgga aattgccggg
     3721 caaattactc aacgcacggt gctcgacgct ctcgaggggc gttttccgat gctgtgcgga
     3781 accattcgcg atcacgccac gcaaaagcgt cgcgcctttg tgcgcttctt cgcttgcggg
     3841 caggatctct cccatgagtc gcccgatgcg atgctgcccg ctgccatagc cagcggcgcc
     3901 gagccgttca tgatcatcgg cgcgatcgcc ggaggatagc ttttgaaaca atatgaaggg
     3961 tcaaatcgtg ctacttggct ttgaggtatg tgctatgttc tggaatgcgc caaaattcac
     4021 atgattaaga aagtcttcat tgctcttgcc gcgttgatta tcggctttgt cgtgatcgtc
     4081 gcgctgcaac cctccgagtt tcgcgtgtcg cgttcgataa acatcgccgc gcctgctcaa
     4141 gccgtgttcg aacaggtgaa cgactttcac aaatgggatg cctggtcgcc ctgggcaaaa
     4201 cgcgacccca acatgaggca aacttacgaa gaggcgcccg ccggcgtcgg cgcgagctac
     4261 tcgtggtccg gcaacaatga ggtcggcgaa gggcgcatga cgttaacgga aacccgtccg
     4321 aacgaaatgg tccgcgttaa gctcgaattc ttgagaccgt ttgccgcgac cccataccac
     4381 cgagttcaat tttcagccca gcggcaataa aacggcagtt acctggagca tgttcggcac
     4441 caacattttc atggcaaagg ttttcggtct gt
//
LOCUS       DATLCH010000350         5543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8853746, whole genome shotgun sequence.
ACCESSION   DATLCH010000350 DATLCH010000000
VERSION     DATLCH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8853746"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2019
                     /locus_tag="VMZ02_10385"
     CDS             <1..2019
                     /locus_tag="VMZ02_10385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUR72387.1"
                     /translation="QGAYHINAGEGSVDIFLHDFDFPTEKIKGYPVRILLQGNTVARI
                     EVLPSKTEKFDLELEPEVVTGLYQRIWQERRVVKLGEVPPLLVKSILAIEDERFFHHM
                     GVDPIGILRAMLVNLRHLSLQQGGSTLTQQLMKNFLLSDERTLSRKFPEAVMALIAER
                     KYPKEVILENYLNEIYLGQRGSQGISGVWEAAQFYFSKPLSELTVGQTALLAGLIRAP
                     NRLSPYRNAEAATKRRNVVLAKLLDDKIINKSQYEAALKERLPQRALVKVTNEAPFYV
                     DYLRRELEQNYAKDVLEKDGLRIFTGLDLRIQKIAEKALTEGLAKLEASHSALKKKSA
                     EESLEGAIVVIRPQTGEIKAMVGGRNYQKSQFNRIFQAKRQAGSVFKPFVFLAAFMHG
                     GQSGMQYFSDTVVNDSQFTWTYDRGQSWQPNNYNNDYFGAVTVRRALESSLNSATARV
                     AQDVGIRRVREVARRLGIESPLPAVPSLSLGSAEVTPLEVAVAFSTLANGGVRARSVA
                     VKHVMDQNTKVLEKRDVKLEQVITPSLAYTMNQLLKGVMDRGTGAPARRWGFTRPAAG
                     KTGTTNDYKDAWFVGYTPELLAVVWVGYDGPAKIGLSGAEAALPIWTEFMKAATANTP
                     VTDFPAPLVPKSETPEVTPPEKCGSATREGELPGEPCPATPVKAAEPV"
     gene            2028..2813
                     /locus_tag="VMZ02_10390"
     CDS             2028..2813
                     /locus_tag="VMZ02_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR72388.1"
                     /translation="MIVSTQSRSKFFAGCAIALALGSSCAPQRPYRTPLPPSGATAPS
                     TGISRPPPLPSTEPPVETSRKPLPQESKIREQDIRATPSSPAGPKDLKEAGKQASINE
                     PRSSSETTPVSPPLADDSSLLAKIVPGITPQRAASLRLTEEGRKLLDAGDPAKAMTRL
                     ERTIVIDSTNGYGYFYLAKAQHRLGHHQESLNLLEVAQSRLGGEAFWMSELHALRGEN
                     YRALGQMQKAESSYHQSLRLNSGNRTASDALARIAGDTPAVTK"
     gene            2826..3824
                     /gene="tsaD"
                     /locus_tag="VMZ02_10395"
     CDS             2826..3824
                     /gene="tsaD"
                     /locus_tag="VMZ02_10395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HUR72389.1"
                     /translation="MLVLGVETSCDDTAAALLQDGKTILANVVSSQDRVHGPYGGVVP
                     ELASRQHIQNILPIVDGALKSAGATLADVDGFAVTYGPGLVGSLLVGISLVKGMSFRT
                     GIPFVGVNHLEAHLLAIHLEDDVPFPYIALLASGGHTLLYHVKGVGQYVHLGGTRDDA
                     AGEAFDKVAKMMGLGYPGGRIIDNLAKAGNPKAVRFPRARFKNSSYEFSFSGIKTAAW
                     HYLKQQGESQWQAHQADIAASFQEAIVDMLVTPTIRAAAANAVKRIVLSGGVAANSRL
                     RAKMTEKGGSEGVQVFCPAPKFCTDNGAMIALTGYHWLKRGRKDGYDLNADADLTL"
     gene            3864..4727
                     /gene="rsmA"
                     /locus_tag="VMZ02_10400"
     CDS             3864..4727
                     /gene="rsmA"
                     /locus_tag="VMZ02_10400"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HUR72390.1"
                     /translation="MPTLYEEVRAMLRDSEFRPRKSRGQNFLIHEHVIDAILRLLDLQ
                     PGDEVVEIGPGLGFVTRRLVERAQKVWAVEIDELLAQRLVAGELGTSGRFHLIYGDIL
                     QVRLPELLPSTKVKLVGNLPYNIATEVLFRIFDWREHFSALVLMVQKEVADRMISDPG
                     SKSYGTLSVWCQVHARISEKIDVSSEAFYPRPKVRSTILKIELLPEPLVCEKNLPVLK
                     GLVRAAFGQRRKTLSNAVGSWLTCAREEIDELLAIHGIDPKRRGETLSVNEFSKLAQA
                     IRQSGLLAATA"
     gene            4841..>5543
                     /gene="mutM"
                     /locus_tag="VMZ02_10405"
     CDS             4841..>5543
                     /gene="mutM"
                     /locus_tag="VMZ02_10405"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HUR72391.1"
                     /translation="MPELPEVETVCRSLRPHLVGRKINRIEVRERRLRLPIDVRQIDL
                     LTGKRIESIDRVAKYILFGLSNSAVWLFHLGMSGKLICTGANIPCQKHDHIVVSLDDG
                     RQMRYHDPRRFGLAVVTHVDELADLPQLRDLGVDPFSVRLTGEYLFSFTRASDRRIRD
                     LLIDQQIVAGLGNIYANEVLAVVRVKPTTRAWRLTRKQVDAIAAAIPELLHDAIQWCG
                     TSFSDYRDADDKSGEF"
BASE COUNT         1247 a         1498 c         1594 g         1204 t
ORIGIN      
        1 caaggcgcat accatatcaa tgctggcgaa ggttcggtcg acatctttct gcacgatttc
       61 gatttcccta cggaaaaaat taaaggttat ccggtgcgga ttttgctcca gggaaacacc
      121 gttgctcgca tcgaggtgct cccgagcaag accgagaagt tcgatctgga actcgagccg
      181 gaagttgtca caggcctgta tcagcggatt tggcaagaac ggcgggttgt caagttgggc
      241 gaagttccgc cgcttctcgt caaatcgatt cttgccatag aagacgagcg gttttttcat
      301 catatgggtg tcgatccgat cgggatcttg cgagcaatgc tggtcaatct gcgtcattta
      361 agcctacaac aaggcggcag taccttgacg cagcagttga tgaaaaattt tctgctcagc
      421 gacgaacgga cgctaagcag gaaatttccc gaagcggtga tggcgttgat cgccgagcgt
      481 aagtatccca aagaagtgat cctcgagaac tatcttaacg aaatttattt gggccagcgg
      541 ggttcgcagg gcatatcggg agtctgggaa gcggcgcaat tttacttctc caagccgctc
      601 tccgaactca ccgtcggcca aaccgcgctg cttgccggcc ttatccgcgc gcccaatcgc
      661 ctgtcgcctt accgcaacgc cgaagccgcc accaagcggc gcaatgtagt cctggcgaaa
      721 ctgctcgacg acaagattat caacaagagc caatatgagg ctgcactcaa agaacggttg
      781 ccccaacgcg ctttggtcaa agtaactaac gaagcgccgt tctatgtcga ttatttgcgt
      841 cgggaactcg aacagaacta tgcgaaagat gtcttggaaa aagacggcct gcggattttc
      901 accgggctcg atctgcgcat tcaaaagatt gctgaaaaag ctctgaccga aggtttggcg
      961 aaactcgaag catctcattc ggcgctcaaa aagaaaagcg ccgaagagag cttggaaggc
     1021 gccatcgtag tgatccggcc gcagaccggc gaaatcaaag cgatggtcgg tggccgtaac
     1081 tatcagaagt cgcagttcaa tcgcattttc caagctaagc ggcaagcggg atctgtattc
     1141 aaaccgtttg ttttcctcgc ggcttttatg catggcggcc agagcggcat gcagtatttc
     1201 tccgatactg tggtaaatga cagccagttt acctggacct acgatcgcgg ccagtcgtgg
     1261 cagccgaata attacaataa tgattatttc ggcgcggtca ccgtgcgtcg ggcgttggag
     1321 agttcactca actccgccac tgcgcgggtg gctcaagatg tcggcattcg gcgcgtgcgc
     1381 gaagtcgccc ggcgattggg catcgaaagt cccttgccgg cggtgccgtc gttatcgttg
     1441 ggctctgccg aagtgacgcc gctcgaagtt gccgtggcgt tttcaactct cgccaacggt
     1501 ggcgtgcgcg cgcgttcagt agccgtcaaa catgtcatgg accagaacac caaggtcctc
     1561 gagaagcgcg acgtcaaatt ggaacaggtg attacaccta gtttggctta caccatgaat
     1621 cagctcctta aaggcgtgat ggaccgtggc accggcgcgc cggcgcgccg gtggggcttt
     1681 acccgcccgg cggctggaaa gaccggtacg actaacgatt acaaggatgc ttggttcgtc
     1741 ggttacacgc cggaattgct cgcggtagtc tgggtaggct acgatggacc ggcaaagatc
     1801 ggtttatcgg gcgccgaggc ggcgttgccg atttggacag aatttatgaa agccgcgact
     1861 gcgaatacgc cggtgaccga ttttccggcg ccgctcgtgc cgaagagcga gacgcccgag
     1921 gtgacaccgc cggaaaaatg cggctcggcg acgcgcgaag gtgaactccc gggcgaacct
     1981 tgcccagcga cgccggtcaa agctgcggag ccggtttagt cttaataatg attgtctcaa
     2041 ctcagagtcg cagtaagttt tttgccggtt gcgccattgc acttgcccta ggctcgagct
     2101 gtgcgccgca gcggccctat cgaacgcctt taccaccgag cggcgcaacg gcgccaagca
     2161 ccggcattag ccgaccacca ccgctaccgt cgactgagcc gccggtggaa acgtcgcgta
     2221 agccgttgcc gcaagagagc aagattcgtg agcaagacat ccgcgcgacc ccgtcatcgc
     2281 cggcagggcc gaaagatctg aaagaagcgg gcaagcaggc aagcatcaat gaaccgcgca
     2341 gttccagcga aacgacgcca gtttccccgc cgcttgccga cgatagttcg ttgctggcga
     2401 agattgttcc cgggataacg ccccagaggg cggcttcttt gcggctaacg gaggagggcc
     2461 gcaagctgct tgacgcaggc gatccggcaa aagcgatgac ccgccttgaa cggaccatcg
     2521 tcatcgactc aaccaacggg tatggctatt tttatttagc caaagcgcag catcgcttgg
     2581 gacatcatca agaatcgctc aatctacttg aagtggcaca gtcacgcctt ggcggtgaag
     2641 cattttggat gtctgagtta cacgcactgc gcggcgagaa ttaccgggcg ctcggacaga
     2701 tgcagaaagc cgagagcagt tatcatcaat ctttgcgtct caattccggc aaccgaactg
     2761 ccagcgatgc gcttgcccgc attgccggcg atactcccgc tgtcactaaa tagtttgcaa
     2821 cctccatgtt ggttctagga gttgaaacgt cttgcgacga caccgccgcg gcgctgctac
     2881 aagacggtaa gacgattctt gcgaacgtag tctcgtcgca ggaccgcgtc catgggccct
     2941 atggtggcgt ggtgccagag ctggcgtcac ggcagcatat ccagaatatt ttacccatcg
     3001 tcgacggtgc cctgaaaagc gcgggcgcga ctttagccga tgtcgatggt tttgccgtga
     3061 cttacggacc gggtttggtc ggctctttgc tggtcggcat atcgctcgtc aaaggcatgt
     3121 catttcgcac cggaattccc tttgtcggcg tgaaccatct ggaagcgcac cttttggcga
     3181 ttcatcttga agacgatgtg ccgtttcctt acatcgcgtt gctcgcttcc ggcggacaca
     3241 ccttgctcta ccatgtcaag ggcgttgggc agtatgttca tctcggcgga acgcgcgacg
     3301 atgccgccgg tgaggccttc gacaaagtgg cgaaaatgat gggattgggt tatcccggtg
     3361 gtcgtatcat cgataatttg gccaaagccg gcaaccccaa ggcggtgcgt ttcccccggg
     3421 caagatttaa gaacagctcg tatgaattca gtttcagcgg cattaaaacc gctgcatggc
     3481 attatctcaa acagcaggga gaatcgcagt ggcaagcgca tcaggcggat atcgccgcga
     3541 gttttcaaga agcgatcgtc gatatgttgg ttaccccgac tattagagcc gcggcggcta
     3601 acgcagttaa acgtatcgtt ctttccggcg gtgttgcggc gaacagccgg ttgcgcgcga
     3661 agatgacaga aaagggtggg agcgaaggtg tgcaggtgtt ttgtcccgcc ccgaagtttt
     3721 gcacggataa tggggcgatg atcgcactga ccggttatca ctggcttaag cgcggccgaa
     3781 aagacggtta cgatctcaac gccgacgccg atctgaccct ctaacacttt cttagaagtg
     3841 aattgcgata agcgctgctg aggatgccga ccctctatga agaagtgcgc gcgatgctgc
     3901 gcgatagcga gtttcggccg cgcaagagcc gcggccagaa ttttttgatc cacgagcatg
     3961 tcattgacgc gattctgcgc ttgctcgatt tgcagccggg tgacgaagtc gtcgagatcg
     4021 gaccgggcct cggatttgtc acgcgccgtt tggtcgagcg cgcacagaag gtgtgggcgg
     4081 tggaaatcga tgaacttctg gcgcaacgac tggtcgctgg ggaactcggg acaagcggaa
     4141 ggtttcatct gatctacggc gacattctcc aagtgagatt gccggagcta ttgccgagca
     4201 cgaaagtaaa actcgtcggc aacctgccat acaacatcgc caccgaagtg ctgtttcgaa
     4261 ttttcgactg gcgcgagcac ttcagtgcgc tggtgctgat ggtgcaaaaa gaagtagccg
     4321 accgcatgat tagcgatcca ggctcgaagt cctacggcac gctgtcggta tggtgccaag
     4381 tgcacgcgcg tatcagcgaa aaaattgacg tctcgtccga agccttttat ccacgcccga
     4441 aggtgcgctc aacgattcta aaaatagaac tgctgccgga gcctctggta tgcgaaaaga
     4501 atctcccggt gctaaaaggg ctggtacgcg ccgcctttgg ccagcgccgg aaaactttaa
     4561 gtaatgcagt aggatcttgg cttacctgcg cgcgcgagga aatagacgaa ctcctggcga
     4621 tccacggtat cgaccctaaa cggcgcggtg agactctgag tgtcaacgaa tttagcaaac
     4681 tggcgcaagc gattcggcag agtggattgc tcgcagccac tgcgtgagca ttggcgtcat
     4741 tgtagtcaat ccgtcctgtc attcagtttt acatactacg aaggccaaac actcatttca
     4801 ggtaaaccca cattctgtaa ttgatcgctg gcgactcact atgcccgaac tccccgaagt
     4861 cgaaactgtc tgccgcagct tgcggcccca tctcgtcgga cgaaagatca atcgcatcga
     4921 agtgcgcgag cggcgtctgc gtttgccgat tgatgtcagg caaatcgatt tgttgacggg
     4981 aaagcgcatt gaatcgatcg atcgagttgc caaatacatt ttgtttggtc tctcaaacag
     5041 cgccgtgtgg ctgttccacc tggggatgtc cggcaaactt atttgcactg gtgcgaatat
     5101 accgtgccag aagcacgacc atattgtcgt cagtttggac gacggccggc aaatgcgtta
     5161 ccacgaccca cgccgatttg ggcttgccgt cgttactcat gttgacgagc ttgcagatct
     5221 gccccagcta cgcgatctcg gagtcgatcc gttctccgtg aggcttaccg gcgagtattt
     5281 gttcagtttc acccgcgcgt ccgaccggcg tatacgcgat ctgttaattg atcaacagat
     5341 cgtcgccggg ctcggtaaca tctacgccaa cgaagtgctc gctgtagtgc gcgtcaagcc
     5401 gacgacgcgg gcttggcgtt taacgcgtaa acaggtggac gcgatcgccg ccgctattcc
     5461 tgaactactg catgacgcga tccaatggtg tggcacgtcg ttctcggact atcgcgacgc
     5521 ggacgacaag tcgggagaat ttc
//
LOCUS       DATLCH010000351         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7957694, whole genome shotgun sequence.
ACCESSION   DATLCH010000351 DATLCH010000000
VERSION     DATLCH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7957694"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VMZ02_10410"
     CDS             <1..395
                     /locus_tag="VMZ02_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72392.1"
                     /translation="QFFDRQRSSVTKRQQRVGQYAWLLLIATAGSFIWLYNYTVDKTV
                     ALNRISSLQTLGAQEVKDLVLSVTLIDGSNVKYVIKSPAPNQKTDVAAKDTVSTEKVS
                     SWELEKLTTAVSVGDSALPLGVALKIAN"
     gene            503..793
                     /locus_tag="VMZ02_10415"
     CDS             503..793
                     /locus_tag="VMZ02_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72393.1"
                     /translation="MVDDTKSLFVGIKISRALQNDLDSPAPGTKHYFEGSENKEYLQI
                     IDMGEEKFIGRYIKSGYPASGITDVSRNICSIIKLIARGRRIEENEVHIYSS"
     gene            901..1341
                     /locus_tag="VMZ02_10420"
     CDS             901..1341
                     /locus_tag="VMZ02_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72394.1"
                     /translation="MEEKREINLRDGTVVRHKNKGYEGKIEGTTAIKACFTRGGMLLD
                     APMSKEVFQYRVVVTGEVSRCIAPAEDLEILDATSAIVCVRCQRGFNTKPGLAGKPGG
                     RCACGGWICPACQGCRVEDPAEDKKKACLNQRKRFMRKAASLKK"
     gene            complement(1729..>2047)
                     /locus_tag="VMZ02_10425"
     CDS             complement(1729..>2047)
                     /locus_tag="VMZ02_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HUR72395.1"
                     /translation="YDAVLLSNIIHGENFARNAALMRKLAVNLNSRGRLIIKDHILAD
                     SRAEPVVGAIFSLLMLLTTDGGRCYSFAEIDAWMKQAGLNHVEQLDLPPPLTSSLVIA
                     ARG"
BASE COUNT          477 a          519 c          609 g          442 t
ORIGIN      
        1 gccagttttt tgaccgccag cgttccagtg ttacaaaacg acaacagcgt gttggccagt
       61 acgcctggtt gctgctgatc gccacggcag ggtcgttcat ctggctttat aattataccg
      121 tcgataagac agtggcgctc aatcgcatct cttcgttgca gacgttgggg gctcaagagg
      181 tgaaggattt ggtgctctcg gtaacgctga tcgatggcag taacgtcaag tacgtcatca
      241 agtctcccgc gccaaaccaa aaaaccgacg tcgcggccaa agatacggtg tcgaccgaga
      301 aggtgtcatc ttgggagttg gagaaattga cgacggccgt gagcgtcggt gatagcgcct
      361 tgccgctcgg cgtcgctttg aaaattgcga actaagcgaa gagctggcgg ttttcgactg
      421 cggcagcgcg atagcccgga gggggtgcgg gtcgggcttt gatgttgctg tgagagttgc
      481 aagtttgaaa gggagcagtg ggatggttga cgatacgaag tcattgttcg tcgggatcaa
      541 aatatcccga gcattgcaaa acgacctcga tagcccggca ccgggaacga aacattactt
      601 cgaaggcagc gagaacaaag aatatcttca gatcatcgac atgggcgagg agaaattcat
      661 cggccgctac attaaaagcg gctaccccgc ctctgggatt acggacgtga gccgcaacat
      721 ttgcagtatt attaaactga ttgcccgtgg gcgccgaatc gaggagaatg aggtgcatat
      781 ctactcctcg tgagctgtaa caactcgggc acgggttggg cgtgttaaca tcagctataa
      841 caatagcttc aatcgttcga aaagcggccc atataacgag aaagaggtat cgaggtatct
      901 atggaagaaa agcgtgagat caatctgcgc gatggtacgg tagtgcgcca taaaaataag
      961 ggctatgaag ggaaaatcga aggaaccacg gcaatcaagg cgtgcttcac tcgcggcggg
     1021 atgctattgg acgcgcccat gtcgaaggaa gtctttcagt atcgcgtcgt cgtcaccggc
     1081 gaagtctcgc gatgcatcgc gcccgctgag gatttggaga tactcgatgc cacgtcggca
     1141 attgtttgtg tccgctgtca aaggggtttt aacaccaagc ccggtttggc gggcaaaccc
     1201 ggcggacggt gtgcctgtgg cggctggatc tgcccagcgt gtcagggatg tcgtgtcgaa
     1261 gaccctgccg aagataaaaa gaaggcgtgc ctcaaccagc gcaagcgttt catgcgcaag
     1321 gccgccagcc tgaagaagta gacgccagaa atgctgcgtc tgatgagggc ttaactctct
     1381 tgtttcgcat actcgcagcg cgggcaggcg gcgcctacca ataccggcca accgcagtcg
     1441 ttgcaagtag tcactggctc gcccaacatg ccacgaacca atgaaccgca gcgccggcca
     1501 ccgcatggac cggtgccgac gccggtgcgc ttgctgattt gttcgaaggt ctgggcgccg
     1561 gattgaatcg ctttggcgac ggtacctcga cggatcttgt tacagatgca tgccggtttc
     1621 aggttgtcga tgaccgcttg ctgtctagct tttagttctt gctcgtcgat ggccggtacc
     1681 ttgcctttac tggttgataa tcgcagctta ccacatatcg gcgcggaact aaccgcgcgc
     1741 agcgatgacc agagacgacg tcagtggcgg cggcagatcg agttgctcaa cgtggttgag
     1801 tcctgcctgt ttcatccaag cgtcgatctc ggcgaagcta taacagcggc cgccgtccgt
     1861 ggtcagcaac atgagcaaac tgaagattgc gccgaccaca ggctcggcgc ggctgtcggc
     1921 gagaatgtga tctttaatga tcaagcgtcc acgcgaatta agattcaccg ccagcttgcg
     1981 catcagcgcg gcgtttcgcg cgaaattctc accgtgaatg atgttcgata gcaagactgc
     2041 gtcatag
//
LOCUS       DATLCH010000352         3137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7960901, whole genome shotgun sequence.
ACCESSION   DATLCH010000352 DATLCH010000000
VERSION     DATLCH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7960901"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VMZ02_10430"
     CDS             <1..775
                     /locus_tag="VMZ02_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HUR72396.1"
                     /translation="YYEEILKSEKAGRINFVVSRAAKSYVLEQIHRDKPLSPPGAQAL
                     YSDLGFMVLAEALETISSSGLDRFCQERIFKPLGLRSTGFVDLTQLRQRRLQPVEEMI
                     APTANCSWRRKFLCGEVHDDNAYAMGGVCGHAGLFSSARDVHAFLLRMNRCLQGKDDF
                     LPKAVIEEFLTKDVSVPNSTYALGWDTPSPANSSSGTLFSPRSVGHLGFTGCSMWWDI
                     EKNCHVILLSNRVHPTRKNEKIRDFRPHVHDLIMKELFP"
     gene            772..2181
                     /gene="mpl"
                     /locus_tag="VMZ02_10435"
     CDS             772..2181
                     /gene="mpl"
                     /locus_tag="VMZ02_10435"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HUR72397.1"
                     /translation="MNDLPAAGSHIHLTAICGVGMASLAGLLKARGYRITGSDQNIYP
                     PMSTYLADIGIHILSGFRAEHLVPRPDLVVIGNAVSRNNPEAEAVLAQEIDYISFPQA
                     LGRFLIGTRQSLVVAGTHGKTTTSALAAWVLASAGLDPGFFVGGVPLNFGSGWNAGTG
                     EYVVLEGDEYDTAFFDKGPKFLHYRPNNVILTSIEFDHADIYRDLAHVKSAFVKLIDI
                     IPVDGSLIVCSDYPEALAVARTVNAQCTTYGESGDWTVQNVKFAQGRSFFETCFKGQS
                     QGLIEAAVIGRHNVKNALSVYVLGRTLGISHDRLLGGFRTFQGVKRRQEVRGEKRGVL
                     VIDDFAHHPTAVRETIEAVRGAYAGRSVWAIFEPRSNTSKRNIFEKEFAAALALADRT
                     IVAGVYQPEKVPEGQRLSVEGLVGEINRVGGAERALTIADSAAIADYVARNSRSGDIM
                     LVMSNGGFDGVHDRILQAL"
     gene            complement(2178..2405)
                     /locus_tag="VMZ02_10440"
     CDS             complement(2178..2405)
                     /locus_tag="VMZ02_10440"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HUR72398.1"
                     /translation="MTLLRLAFLFIIIYIVYSALKPLLQGGAKPANPAQLGEQMVLDP
                     QCQTYVPKSDAVIRQGKFFCSEECAQRYLAG"
     gene            complement(2439..2942)
                     /gene="folK"
                     /locus_tag="VMZ02_10445"
     CDS             complement(2439..2942)
                     /gene="folK"
                     /locus_tag="VMZ02_10445"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HUR72399.1"
                     /translation="MANAITHQVYIGIGSNVGNKRENFFEAVERVGKLPDTRVIKESS
                     LYESEPLGDAKDWYVNGAIEIETKFKPEALLEKFKNIERAMGRKKVKKRWGARIIDLD
                     ILLYDLAVVKKKNLRIPHPEMPSRKFVLIPLSEIAPQVIHPELGVTISELLINVKDDK
                     KIHLFHS"
BASE COUNT          691 a          823 c          870 g          753 t
ORIGIN      
        1 ctactacgaa gaaattttaa aaagtgaaaa ggcagggcga attaactttg tcgtcagccg
       61 cgctgctaag agctatgtct tagagcagat ccaccgcgat aaaccgctta gtcctccggg
      121 cgcgcaggcg ctctatagcg acctcggctt tatggtgctg gcggaagctc ttgagaccat
      181 atcgagcagt ggtctggacc gcttctgcca ggagcggatc tttaaacctc tcggactgcg
      241 ctcgaccggt ttcgtcgatc ttacgcagct tcgccaacgg cggctgcaac cggtggaaga
      301 aatgatcgcg ccgaccgcaa actgttcttg gcgcaggaaa tttttatgcg gtgaggttca
      361 tgatgataac gcctacgcca tgggcggggt gtgcggccac gccggacttt tttcctcagc
      421 gcgtgacgta cacgcttttc tattgcgaat gaaccgctgt ttacaaggca aggatgattt
      481 cttgccgaag gccgtgatag aagagttttt gactaaagat gtttcggtgc cgaattcgac
      541 ttatgctttg ggttgggaca cgccatcgcc agcaaattca tcgagcggta cgctattttc
      601 gccgcgttcc gtcggccacc tcggtttcac cggctgttcg atgtggtggg atattgaaaa
      661 gaattgtcac gttattttgt tgagcaatcg ggtacaccca acgcgaaaga atgaaaagat
      721 tcgcgatttt cgcccccatg tgcacgacct aataatgaaa gagctttttc catgaacgat
      781 ttgccggctg ccggaagcca cattcatctg acggcaatat gcggtgtcgg catggcctcg
      841 ctggctgggc tactcaaggc gcgcggttat cgcattaccg gttcggatca aaatatctac
      901 ccgccgatga gcacttattt ggcggacatc ggtattcata tcctcagcgg ctttcgtgcc
      961 gaacacctgg tcccgcgccc cgatctcgtg gtcatcggca atgccgtgtc gcgcaataat
     1021 ccagaggccg aagcggtatt ggctcaagaa atcgactaca tctcttttcc tcaggcgctg
     1081 ggccgtttcc ttatcgggac gcgccagtcg ctggtggtcg ccggcacgca tggaaaaacg
     1141 acgacgagcg cattggcggc ctgggtgctt gctagcgccg gactcgatcc gggatttttc
     1201 gtcggcggcg tgccgctcaa tttcggcagc ggttggaatg ccggaacggg cgagtatgtc
     1261 gtgctcgaag gggatgagta tgacaccgcc tttttcgaca aggggccaaa gtttctgcac
     1321 taccgtccga ataacgtcat attaaccagc atcgagtttg atcatgccga catttaccgt
     1381 gatctagctc atgtgaagag cgctttcgtt aagctcatcg acatcattcc cgtcgatggc
     1441 tcgctgattg tgtgcagcga ctacccagag gcactcgcag ttgcgcgcac cgtgaacgct
     1501 caatgcacta cctacggcga atccggcgat tggaccgttc aaaacgtgaa attcgcccaa
     1561 gggcgcagtt tcttcgagac gtgtttcaag ggtcagtcgc aaggcctaat cgaggcagcg
     1621 gtcatcggtc ggcacaatgt taaaaacgcc ctgtccgtct atgtattggg tcgaacgtta
     1681 gggatatcgc atgatcgact gctcggcggt tttcgcactt tccaaggagt caaacggcgc
     1741 caagaagtgc gtggtgaaaa gcgtggtgtc ttggtgattg atgacttcgc ccatcaccca
     1801 accgccgtac gcgaaaccat cgaggcggta cgcggcgcct acgccgggcg cagcgtttgg
     1861 gcgatcttcg agccgcgcag caataccagc aagcgcaaca tttttgaaaa agaattcgcc
     1921 gcggcgttgg ccctggccga caggacaatc gtcgccggtg tttaccaacc tgaaaaagtg
     1981 ccggaaggtc aacgactgtc agtcgaaggc ttggtcggtg aaattaaccg cgtcggcggt
     2041 gcggagcggg cgctgacaat cgcggactcg gcggcgatcg ccgactatgt ggcacgcaac
     2101 agccgctccg gagatataat gctggttatg tccaacggcg ggtttgacgg tgtccatgat
     2161 aggatcctgc aggctctcta gccggctaaa tagcgctgcg cgcattcttc gctacagaaa
     2221 aactttccct ggcgtatcac agcatccgat ttcggcacat aggtttggca ttggggatca
     2281 agcaccattt gctcgccgag ttgcgccgga ttagccggtt ttgctccacc ctgtagcagc
     2341 ggcttcaagg ccgagtaaac gatataaata atgatgaata ggaaagctaa acggagcaga
     2401 gtcatagcgc tcctagcgac tggggaggag tgctcctcct aggaatgaaa aagatggatt
     2461 tttttgtcgt ctttgacgtt gatcaggagc tccgagattg tgacccccaa ttctggatga
     2521 atcacttgcg gcgcgatttc gctcaatggg attaagacaa atttacggct gggcatttcc
     2581 gggtgcggga tgcgcagatt ttttttcttc accacggcca aatcgtagag caaaatatct
     2641 aaatcgatga ttcgagcacc ccaacgcttc ttgacctttt tgcggcccat ggcacgctcg
     2701 atgttcttga atttctcaag cagcgcctcc ggtttgaatt tggtttcaat ttcaatggca
     2761 ccattgacgt accagtcttt ggcatcgccc aggggttcgc tttcgtaaag cgaagactcc
     2821 ttgatcacac gtgtgtcggg cagtttgccg acacgttcga cggcctcgaa aaagttttcc
     2881 cgcttattgc cgacattgga gcctatgccg atgtaaactt ggtgagtgat ggcgttggcc
     2941 atggtgccgt ttgggaaagg ccgtactaga gcttaacgcg gcgaatcctt tccgcaacct
     3001 cccgaagtcg ttctttgtcg acgcagacag taaatcggac atagccttcc ccggcatccc
     3061 cgaaaccatt acctggagtt gtgaccacgc ccgcatcatc gagcaatttg gcagtgaaat
     3121 cggccgatga tagtcct
//
LOCUS       DATLCH010000353         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6479980, whole genome shotgun sequence.
ACCESSION   DATLCH010000353 DATLCH010000000
VERSION     DATLCH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6479980"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VMZ02_10450"
     CDS             <1..874
                     /locus_tag="VMZ02_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HUR72400.1"
                     /translation="VDGAPYKLGGECVWGRGACEQKGSLAAMMAAVKFIGSNNVELPS
                     DLYFVVSTAGETGKHDSLAYVLDNANVNAEWCIIDGPPEIQLGNKGRVDVLVVVKGKQ
                     AHSSRPWEGINAIDGAMQVLDRLKPLMPTPEDKHHPELGNVSLTTNAIESFPKATHTI
                     QSECRVMLDRRLLPGDDPAKAIEQLKEAIGTIEPFEIEVQPRDFMYPSEVGKDAEVVQ
                     VLEQGIRTMLNYEPKFSYSTAANDTGLFNHRGIQAINYGSRDIRFQHTDHDLVALNNV
                     FNAAKVFAFLGIHR"
     gene            complement(985..1677)
                     /locus_tag="VMZ02_10455"
     CDS             complement(985..1677)
                     /locus_tag="VMZ02_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HUR72401.1"
                     /translation="MTIQEKLALACRILAMQGHNDMIYGHVSARTERPDQYWIKGSGI
                     GLEEATADDMVLIDFEGNTLAGKRKRHNEFPIHSEVYRTNPGLMCVIHTHPTYSTIIA
                     SSDKSILPITNLSCAFYPPAIDKFEESSDLIVTPAQGTSVAKLLGEHKIVLLRNHGIV
                     VAGPNIEETCIRGVLLEHSAKTQVKAASMGAFTWATDADALLKRKRIYRPDAMLNLWE
                     YYLRMLQKHESK"
     gene            complement(1704..2039)
                     /locus_tag="VMZ02_10460"
     CDS             complement(1704..2039)
                     /locus_tag="VMZ02_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72402.1"
                     /translation="MKHKINSQGMRRIISLSFVALFQFSAAPVWADHDRLRNEVLQFH
                     SFLQNRPKVSSELRNNPQLVNSKRYLDNHDDLEKFLKRHPRVKQEIVAHPSRVFGTYY
                     RENHARWRH"
     gene            complement(2068..2838)
                     /locus_tag="VMZ02_10465"
     CDS             complement(2068..2838)
                     /locus_tag="VMZ02_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR72403.1"
                     /translation="MIHGIDHLVIVVKDLDQAARDYQQLGFTVVPGGQHPVGSHNVLI
                     SFADGSYLEIIAFYREAADHRWWEPLQKGERLVDFCLQTDDLHGDTKKLQDAGVAINN
                     PVPWSRKRPDGYELKWLLSLATGSHRGVAPFLIEDVTPRTERIPQQFSQANQITGIDK
                     LVVAVGELGQVDKWYSALLGGKGESFTDTNLGADGLRFAIGAHRIEFVTPRDAASPLI
                     QWLREFGPSPYSAVLKSANHSTQMLDHNLTHGARLLLS"
BASE COUNT          656 a          774 c          841 g          668 t
ORIGIN      
        1 cgtcgacggc gcgccgtaca aactcggcgg cgaatgcgtt tggggacgcg gtgcttgcga
       61 gcaaaagggc agcttggcgg cgatgatggc cgcggttaaa ttcatcggta gcaacaacgt
      121 cgaactgccg agcgatctct atttcgtcgt cagcactgcc ggcgaaaccg gcaaacacga
      181 ctcgttggcg tacgtgctcg ataatgccaa tgtcaatgca gagtggtgca tcatcgacgg
      241 tccgccggag attcaactag gcaacaaagg ccgggtcgac gtgctggttg tcgtcaaagg
      301 caaacaggct cacagcagcc ggccatggga aggcatcaac gccatcgacg gcgctatgca
      361 ggtgttggat cgtttgaagc cgttgatgcc gacgcccgag gataaacatc atcccgagtt
      421 gggcaacgta agcctcacca ccaatgcgat cgaaagcttc cctaaggcca cccatacgat
      481 tcagagcgaa tgccgggtca tgctcgaccg ccggttgctt cccggcgacg atcccgccaa
      541 ggcaattgag cagttgaagg aagccatcgg cacgatcgag ccattcgaga tagaagtgca
      601 gccgcgcgac ttcatgtacc cgagcgaagt cgggaaagac gccgaagtag tgcaggtttt
      661 ggagcagggg atacggacga tgctcaatta cgagccgaag ttttcctatt caacggccgc
      721 caacgacact gggctgttca atcatcgcgg tattcaagca atcaactacg gctcgcgaga
      781 tattcgattt cagcacacgg accacgattt agttgcatta aataatgtct tcaacgccgc
      841 caaggtcttt gcgtttttgg ggatacatcg ataggcgcgt aaagcgcgcg gtttaaaacg
      901 ttcaagcagt tcaaatcgtt caagacgtcc ggagacaacg gtttgaacgt tttgaacgga
      961 ggctgtcatc agccgattga acgtttattt gctttcgtgc ttctgcagca tgcgcagata
     1021 atactcccaa agattcagca tggcgtcggg ccgataaatc cgttttctct ttaacaatgc
     1081 gtcggcgtcg gtcgcccagg tgaaggcgcc catggaggcg gctttgacct gggtcttggc
     1141 gctatgttcg agcagcacgc cgcgtatgca ggtctcctcg atgttcggac ccgcgacgac
     1201 gatgccgtgg ttgcgtagta ggacgatctt gtgttcgccc aacaatttcg ccaccgaggt
     1261 gccttgtgca ggagtgacga tcaagtctga ggactcttcg aacttatcga tggccggtgg
     1321 atagaaggcg cagctcaagt tggtgatcgg caaaatcgat ttgtctgacg acgcgatgat
     1381 cgtcgaataa gtcgggtgtg tatggatcac acacatgagg ccgggattgg tccggtagac
     1441 ttcactatga atcggaaatt cgttgtgccg tttgcgcttg ccggcgaggg tgttgccttc
     1501 gaaatcgatg agaaccatgt cgtcggcggt ggcctcttcc aggccgatgc ccgatccctt
     1561 gatccaatat tggtcgggtc gctcggtgcg cgccgaaaca tggccgtaga tcatgtcgtt
     1621 gtgtccttgc atcgccagga tgcggcaggc caacgccagt ttttcttgta tggtcatcga
     1681 tgattctcct cggactgggg tagctaatgg cgccaacggg cgtggttctc tcgataatag
     1741 gtaccaaaca ctcggctcgg atgtgcgaca atttcctgct taacccgggg atggcgcttc
     1801 aagaactttt ccaggtcgtc gtggttatcg agatacctct tactgttgac cagttgggga
     1861 ttgttacgta actcgctcga caccttgggg cgattttgca ggaaactgtg aaactgcaga
     1921 acttcgttgc gcagccggtc atggtcggcc cacacaggcg cggctgagaa ctggaacagg
     1981 gcgacaaacg aaagcgaaat tatcctgcgc attccttgcg agttgatctt gtgcttcatc
     2041 gattcagcgt gtccgcggcg aacaatatta cgaaagcagc aatcgagctc catgcgtcag
     2101 gttatgatcg agcatctgcg tggaatggtt ggccgacttt aatactgccg agtagggcga
     2161 cgggccaaac tcacgcagcc actgaatcag gggactggcc gcatcgcgcg gagtcacgaa
     2221 ctctatacga tgcgcgccga tcgcaaagcg caggccgtcg gcgccaaggt tagtgtcggt
     2281 gaacgattca cctttgccgc caagcagagc gctgtaccac ttgtcgactt gcccaagctc
     2341 gccgactgcg acgaccaatt tgtcgattcc ggtgatctga tttgcctggc taaattgttg
     2401 gggtatccgc tcggtgcgcg gagtcacgtc ttcgattaga aagggcgcca cgccgcgatg
     2461 actgccggtg gccagtgaca gcaaccactt gagctcataa ccgtccggcc gtttgcgcga
     2521 ccagggaacc ggattgttaa tcgccacgcc ggcgtcctgc agtttttttg tatccccatg
     2581 cagatcgtcg gtttgcaaac aaaaatcaac cagacgctcg cccttttgca gcggctccca
     2641 ccagcgatga tcggctgctt cgcgatagaa cgcgatgatt tccagataag aaccgtctgc
     2701 gaacgagatc aacacattat ggctgcccac cgggtgttgt ccgccgggta cgacagtaaa
     2761 tcccaattgc tgatagtctc gcgccgcttg atcgagatcc ttaacgacga tgactaaatg
     2821 atcgataccg tgaatcatgg attgagctga ctggtgtgtt cgcttgatct gccgcgagct
     2881 agcgtatttt gagttcctgt tgcacttctc gcagaaaact aaaatcgaca gcttgagac
//
LOCUS       DATLCH010000354         4907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_11118054, whole genome shotgun sequence.
ACCESSION   DATLCH010000354 DATLCH010000000
VERSION     DATLCH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11118054"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..152)
                     /locus_tag="VMZ02_10470"
     tRNA            complement(76..152)
                     /locus_tag="VMZ02_10470"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Ile,seq:cat)
     gene            complement(208..2079)
                     /gene="rpoD"
                     /locus_tag="VMZ02_10475"
     CDS             complement(208..2079)
                     /gene="rpoD"
                     /locus_tag="VMZ02_10475"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HUR72404.1"
                     /translation="MARPASRKTPARTPKTRAETKPALNRTKNASDAAGEPVEKKNLK
                     EVKKLIDLGKEKGYLTYDDVNDMLPAEVVSPDQIDDVMSIFGEMEIEVVDANQRVTLG
                     SAGDELVEEEDDEKETEDADNDVVGKTGDPVRMYLREMGTVSLLSREGEVEIAKKIEN
                     GENRVLDEVLSSPLALTHILDLGEKLALHEVRVREIIKEEGDEESEDFEEEEVHEKRL
                     LDQIGKIRRTVNEREKIRNQLDGKRVSAQRRQSLVDSVTKYNDRIINGLKNLQLNRKQ
                     TEAIAEGLKRAMERMQAMERRVKDYEHRLGKSGSEILKMVAAAEAQKKNWPKLVSTFR
                     MGHDVILHMAEEIRNVRRDVRHIEKDLEMPSEEIKRRVWSIIDGETRANLAKKELIEA
                     NLRLVVSIAKKYTNRGLQFLDLIQEANIGLMKAVDKFEYQRGYKFSTYATWWIRQAIT
                     RAIADQARTIRIPVHMIETINKLIRTSRYLVQEIGREPTPEEIASKMEIPLDKVRKVL
                     KIAKEPISLETPVGEEEDSHLGDFIEDKRIITPSDAVVNINLQEQTRKVLATLTPREE
                     QVLRMRFGIGEKSDHTLEEVGQKFTVTRERIRQIEAKALRKLRHPSRSKRLKSFMEN"
     gene            complement(2007..3809)
                     /gene="dnaG"
                     /locus_tag="VMZ02_10480"
     CDS             complement(2007..3809)
                     /gene="dnaG"
                     /locus_tag="VMZ02_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HUR72405.1"
                     /translation="MISQEKIAEIRNRASIVEVISDYVTLKKSGRNHMGLCPFHGEKT
                     PSFTVNEEKGIFHCFGCHAGGGVFQFLMQYDHVSFPEAVEQLAKRYGIVIERTEGRNF
                     QRDLGQRENLYRINECAAANYQRILLDHPQGRVALEYLRRRGIDDATARRFMLGFAPS
                     GGSGLVGLLRKESLALVDAIKLGLVTQRDPQQYYEKFFSRVMFPITNAAGKIVGFGGR
                     VIDQGMPKYLNSSETPLFHKGSTVYGLHEAKESIRQTDRVVLVEGYLDVIALAQHGIG
                     CAVATLGTALTVEHVRALSRYTKNIIALFDGDDAGLKAAARSFEIFIEAGLLGRAALL
                     PKGEDPDTFVRKHGKEAAENLLAQAVPLADYYFSWLQKRYGSTLEGKSQTASEIGRLL
                     TKVNNPFEVDLLIRRAADLGVREELLRRPLAQAVNPRPLSTSRPIAAALAPDDSRDDI
                     AQRSLVGLLLRVPTLLDKLARDARVRLYFSDKWRPVVDAITLEWQETGNIDIGAISQK
                     LPESLVSEVAALVLEAENRSDAECANMATDCLTHLQRKYLKSQERDLRIAIRAAEEKR
                     DDVAKRERILEWQDLLQEKRQLERRRLELKPSPR"
     gene            complement(3819..4352)
                     /locus_tag="VMZ02_10485"
     CDS             complement(3819..4352)
                     /locus_tag="VMZ02_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HUR72406.1"
                     /translation="MNWVDLILLAVLILFGLRGFFRGFFREILSLAGLIVGFLVAARY
                     GDVAAQYVAQHWHLSPLLLKGTGFVALFFVIYFGFNLAGWLLHRSEKMLFLKTINRAG
                     GIAVGVGKGAALAALAIFLLNSSSLLSHPTRESFESAYLVRPLSQFGESLIKAGKQRM
                     FTGDNSAPPPAHKRTSI"
     gene            complement(4477..>4907)
                     /locus_tag="VMZ02_10490"
     CDS             complement(4477..>4907)
                     /locus_tag="VMZ02_10490"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HUR72407.1"
                     /translation="IQQAVRAAMKGGDTVTLSTLRLLLAAMQNEEIRSRRALSAEEIQ
                     KVIGTLCKQRAEAIELYRKGGRQDLATKEEAELTVLKGFLPQQFSDEEIKSAIQASID
                     EAGAKGLADLGKVMKLVMPKVAGRSDGKRVSDLTKALLSG"
BASE COUNT         1007 a         1438 c         1277 g         1185 t
ORIGIN      
        1 tgccgctcgc gccaagaacg tctccttccg ggggtaaaac ctcccggaaa ggagagttct
       61 tgacactgtg caatttggtg ggcccacctg gactcgaacc agggaccaac cggttatgag
      121 ccggtggctc taaccaactg agctatgggc ccttaagaat cgattgaaat ttgccggatt
      181 cgtgcttgtg gcgatgcacg tcgcccttta attttccatg aaactcttga gccgcttgct
      241 ccggctcggg tggcgcagtt tacgcaatgc tttggcttcg atttggcgga tgcgctcgcg
      301 cgtgacggta aatttctgcc caacctcttc cagagtgtga tcagactttt caccgatgcc
      361 aaagcgcatt cgcaaaacct gctcttcacg cggcgtcaga gtcgccagca ctttgcgcgt
      421 ctgttcctgc aagttgatgt tcaccacggc gtcggagggc gtgatgatcc gtttgtcttc
      481 gataaaatcg cccaaatgac tgtcttcttc ctctccgacc ggcgtctcta aagagatcgg
      541 ctccttggcg attttcaaaa ccttgcgcac cttatcgagc ggaatctcca tcttactggc
      601 gatctcttcc ggcgtgggct cgcggccaat ctcttggacc aggtaacgcg aggtgcgaat
      661 tagcttgttg atcgtctcga tcatatgcac cggaatgcga atggtgcgcg cctggtcggc
      721 gatggcgcgg gtaatagctt ggcgtatcca ccaagtcgca tatgtggaga atttgtaacc
      781 gcgctggtat tcaaacttgt cgacggcttt catcaaaccg atgttggcct cctgaatgag
      841 gtcgaggaac tgcaggccgc ggttggtgta tttttttgcg atgctgacga ccagtcgcag
      901 gttggcttca atcaactcct tcttggcaag gttcgcgcgg gtctcaccgt cgatgatcga
      961 ccacactcgc cgcttgattt cctcagacgg catctccaga tctttttcga tatgacgcac
     1021 gtcacgtcgt acattgcgga tctcttcagc catatgcagg atcacgtcgt gccccatgcg
     1081 gaacgtcgac acgagcttgg gccaattctt cttctgcgct tcggccgccg ctaccatttt
     1141 gagaatctca ctgccggatt tgccgagccg atgctcatag tctttgactc ggcgctccat
     1201 ggcctgcata cgttccatag cccgcttaag accctcggcg atggcttcgg tttgtttgcg
     1261 attgagctgc aaattcttca gaccattgat gatgcgatcg ttatatttgg taacactgtc
     1321 gacgagcgac tggcgtcgct gcgccgacac ccgtttgccg tcgagctggt ttctgatctt
     1381 ctcgcgctca ttgacggtgc ggcggatttt gccgatctga tcgagtaagc gtttctcgtg
     1441 gacctcttct tcttcgaagt cttcactttc ttcgtcgccc tcttccttga tgatctcgcg
     1501 cacccgaact tcatgcagcg ccagcttctc gccaagatcg agaatatgag tcaatgccag
     1561 ggggctggag agcacttcat caagtacgcg gttctcgccg ttttcgatct tcttggcgat
     1621 ctccacctcg ccctcgcggc tcagcaatga tacggtgccc atctccctaa gatacattcg
     1681 caccggatcg ccggtcttgc cgacgacgtc attgtcggcg tcctcggtct ctttttcgtc
     1741 gtcctcctcc tcgaccaatt catcgccggc actgccaaga gtgacccgtt gattggcatc
     1801 gacgacttcg atctccatct cgccaaaaat cgacatgaca tcgtcgatct gatcgggcga
     1861 gacaacctca gccggcagca tatcgttgac gtcgtcgtag gtcagatagc ccttctcctt
     1921 gcccaaatcg atgagctttt tgacctcctt caaattcttc ttctccactg gctcgccagc
     1981 ggcgtcggaa gcgttcttcg tcctatttag cgcgggcttg gtttcagctc gagtcttcgg
     2041 cgttctagct ggcgtttttc ttgaagcagg tcttgccatt ctagtatcct ttctctcttg
     2101 gcgacatcat cgcgtttctc ttcggcggcg cgtattgcaa tacgtaaatc gcgctcctga
     2161 ctctttaaat actttcgctg caagtgggtc agacaatcgg tggccatgtt cgcgcattcg
     2221 gcgtcggaac ggttctctgc ctccagaact aaggcagcaa cttcgctgac caaagactcc
     2281 gggagctttt gactgatggc gccgatgtcg atgttccctg tttcttgcca ttccaaagtg
     2341 atcgcgtcaa ctaccggacg ccatttgtca ctaaaatata aacggacgcg cgcgtcccgt
     2401 gcgagtttgt cgagcagagt cggcacgcgc agcaacagtc cgaccagcga acgttgggca
     2461 atgtcgtcgc ggctatcgtc cggggccaga gcagcggcaa tcggacggct cgtcgatagc
     2521 ggccgcggat tgacagcctg ggccagcggc cgacgcaaga gctcttcacg cacacctaag
     2581 tctgcggcgc gacgaatcaa caaatcaact tcaaaaggat tgtttacctt ggtgagcaat
     2641 cggccgatct cgcttgcggt ttggctcttg ccttcgaggg tgctgccata gcgtttttgc
     2701 agccaagaaa aatagtaatc ggccagcggc acggcttgag ccagtaaatt ctcagccgct
     2761 tccttgccgt gcttacgcac gaacgtgtcc ggatcttcac ccttcggcag gagcgccgct
     2821 cgtccgagca agccggcttc aatgaagatt tcgaagctgc gcgccgcggc tttgagaccg
     2881 gcgtcgtcgc cgtcgaataa ggcaatgatg ttcttggtgt agcgcgacaa tgcacgcacg
     2941 tgttcgacgg tcagtgccgt ccccaatgtc gccaccgcgc aaccgatgcc atgctgtgcc
     3001 agagcgatga catcgagata accttcaaca agcacgaccc ggtcggtttg gcggatactt
     3061 tccttggctt cgtgcaaacc gtacactgtc gaccccttgt gaaacagcgg cgtttcactc
     3121 gagttcaaat acttcggcat accctgatca atcactcggc caccgaagcc gacgattttt
     3181 cccgccgcat tggtgatggg aaacatcact ctggaaaaaa atttttcata atactgctgc
     3241 ggatcgcgct gcgtaacgag cccgagcttg atcgcgtcca ccaacgccaa actttctttc
     3301 ctgagcaaac ccaccaagcc ggacccgccc gagggggcaa agcccaacat gaagcgtcgt
     3361 gccgtggcgt cgtctatgcc gcggcggcgc aggtattcca gcgccacgcg gccttgagga
     3421 tgatcgagca ggatgcgctg gtagttagcg gcggcgcatt cgttgatgcg atacagattc
     3481 tcacgctgcc ctagatcgcg ctgaaagttt cggccttccg ttcgttcgat gacgatgcca
     3541 tagcgcttgg ccagttgttc cacagcctcg gggaaactta cgtggtcgta ctgcatgagg
     3601 aactgaaaca caccgccgcc ggcgtggcag ccgaagcagt gaaaaatgcc cttctcttcg
     3661 ttaaccgtga atgacggggt tttctcgccg tgaaatggac aaagtcccat gtggttgcgg
     3721 ccggactttt taagcgtgac gtagtcagaa atcacttcga cgatcgacgc gcggttacgg
     3781 atctcagcga ttttttcctg gctaatcatg aaaacaaact aaatcgacgt tcgcttatga
     3841 gcaggcggag gggcagagtt atcgccggtg aacattcgct gtttgccggc tttgatcaaa
     3901 ctttctccga attgggacaa tggccgcacc aagtaagcgc tttcaaagga ctcgcgagtc
     3961 ggatgagata gcagtgaaga tgagtttaag agaaaaatgg ccagcgccgc cagagccgcg
     4021 cccttgccga cgccgacggc aatgccgccg gctcggttga tagtcttgag gaaaagcatt
     4081 ttctccgaac ggtgcagcaa ccagccggcc aagttaaagc cgaaataaat cacaaagaag
     4141 agtgcaacga acccggtacc cttaagcagc agcggtgaca agtgccaatg ctgcgccacg
     4201 tattgcgccg caacatcgcc gtaccgtgcg gcgaccaaga acccgacgat gagtccagcg
     4261 agtgacaaga tttcacggaa aaaacctctg aagaagccac gcaagccgaa tagaattaag
     4321 acggcgagca gaatcagatc cacccaattc accagctggc acccacctgt ggtggcagcg
     4381 ccgccccgtg gtcgttcacg ccgctcgccg tcttcgcatg atcgatgact tctacaacag
     4441 acctattaca tctgccgaac agccgttagg cttgcgctaa ccactcagca atgccttcgt
     4501 cagatcgctg acccgcttac catcgctgcg tcccgccacc ttgggcatca ctagcttcat
     4561 cactttaccg agatcagcta gccccttggc accggcctcg tcgatactcg cttgaatcgc
     4621 ggacttaatc tcttcgtcgg aaaattgctg cgggagaaac cctttgagta ccgttaattc
     4681 ggcctcttcc ttggttgcca gatcctggcg cccgcctttg cggtaaagct ctatcgcttc
     4741 cgcgcgctgt ttacacaaag tgccgataac tttctggatc tcctcggcac ttagcgctcg
     4801 gcgactgcga atttcctcgt tctgcatcgc cgccaacaac aagcggagag ttgaaagcgt
     4861 gaccgtgtca ccgcctttca tagcggctct cacggcctgc tgtatct
//
LOCUS       DATLCH010000355         3487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_11126693, whole genome shotgun sequence.
ACCESSION   DATLCH010000355 DATLCH010000000
VERSION     DATLCH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11126693"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VMZ02_10495"
     CDS             <1..746
                     /locus_tag="VMZ02_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUR72408.1"
                     /translation="SGMMSLVTDLDDPKVAGVSFVDHTAGIFAAHGIMAALLARSKTG
                     VGQFIDVSLLQVAIAFIESHVSDYLNGGEAVSRENFPKGRIYSLLDCDKKPFMVHLSG
                     HNEAWEGLLKAAELNELIGDPRFSSRKQRSDRHDEIVNLLRSAFVKKRREYWLARLDS
                     HSIPNAPINTIHEVFDNPQIKHMGIPKQIQHPKMGMSNLVGSPINMSGTPPKFFRPAP
                     LLGENTEEVLAKLGYDSNAVKELKASGVI"
     gene            869..2083
                     /locus_tag="VMZ02_10500"
     CDS             869..2083
                     /locus_tag="VMZ02_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR72409.1"
                     /translation="MDFTVSADPPHVVEFRKEVGAWLAEQFKGYEHLRWSASWSTRED
                     AEEYKFRRGLAEKLGAKGWLFPLYPKQYGGAELTVDHQVVLETELFKYGLNLSHTFYT
                     LARIVAPVLIRWGTEEQKAEFLPAITRAQICVWQVLTEPHGGSDIANCQTKAIRDGDH
                     YVVNGQKVMVGHHLPPDYLWTLVCTNPQGKRHENLAWLHIPANLSGITIQHMNMMMGI
                     KNAVFFDNVRVPAKYLIGGENNGWKVASTHMELEHGGDGRIAADPLIERVAEYCRNTH
                     IDGKPIIEDPRVRDLIAEMTIELNTLRRFNQLIYWRRFAKKPHPYGGPQQRYFQRMLR
                     LRNGERLQAIMGADALEQNLSVHEVIDFEYAVRTGPGQLHGGGTLDTDRLIFARRLGL
                     GRPSKESAPETH"
     gene            2135..3256
                     /locus_tag="VMZ02_10505"
     CDS             2135..3256
                     /locus_tag="VMZ02_10505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HUR72410.1"
                     /translation="MDLGLTEDQEMLKKVAADFVKAEVPAHQMTQWYKDKKIFRPELV
                     RKAAELGWLGMMLPEEWGGAGLAPMDCAVVFEQLGHGPVPGPLFTSGILSAQMIFEAC
                     TDAQRKDLLPALCSGKSIVIPAITDKSAYWGPEAVETRLSKTPGGYLLNGTKRFVFDA
                     DAATNFLSAARTEEGKVVFLLVDAKASGITVTQHTGFFVSVFEVRFNNVALSADNLLG
                     SSGASWATLEKALDKALPILSAYQVGATQEVFDMTCEYTNSRVVFGQPIGRFQRVQDH
                     CVDISICLDTARWATYEAIWRIENGMDARAGVHEAKATASDGYYHGTNYAHMVWAGPG
                     TDYSHPMMAHSVLAHTLYQYLGTPGHHKRLMVDALYPRA"
BASE COUNT          794 a          973 c          982 g          738 t
ORIGIN      
        1 tgagcggcat gatgagcctg gtcaccgacc tcgacgaccc taaggtcgcc ggggtgtctt
       61 ttgtcgatca taccgccggc attttcgccg cccacggcat catggcagcg ctattggcac
      121 ggagtaaaac cggagtgggc cagtttatcg acgtctcgct tttgcaagtg gccatcgctt
      181 ttatcgaatc ccatgtttcg gattatttaa acggcggcga agcggtatcc cgggaaaact
      241 ttccgaaagg aagaatttac agcctgctcg attgtgacaa gaaaccgttc atggtccatt
      301 tgtccggcca caacgaagct tgggaaggcc tcctcaaagc cgccgagctc aacgagctga
      361 tcggcgaccc gcgtttctct tcgcgcaaac agcgcagcga tcgccacgac gaaatcgtca
      421 atctgctgcg ctctgctttt gtcaagaagc ggcgcgagta ttggctggcg cgcctcgact
      481 cccatagcat ccctaacgcg ccgatcaaca caattcacga ggtgttcgat aatccacaaa
      541 taaagcacat gggtatccca aaacagatcc aacatccgaa gatgggcatg agcaatctgg
      601 tcggcagccc aatcaatatg tcggggacgc cgccgaagtt tttccgcccg gcgccgctgc
      661 tcggcgagaa cactgaggaa gtgttggcga agttgggtta tgacagcaat gctgttaagg
      721 aattgaaagc aagcggcgta atataagatt ggtgtctagt gacgggcatg atcgttgggc
      781 cgttaatggt ttccattcac tagtcgactc ctgcctagcg ccggaaaccg tgaactcgaa
      841 gctgtgaatt atcgaaagga agtgaattat ggattttact gtaagtgcag atccgcctca
      901 tgtcgtggaa tttcgcaaag aggttggcgc ctggctcgcc gagcagttca aagggtacga
      961 gcacttgcgc tggtcggcct catggtcaac tcgcgaagac gccgaggaat acaagtttcg
     1021 ccgtggttta gctgaaaaac ttggcgccaa agggtggctg tttccgcttt atccgaaaca
     1081 atatggcggc gccgagctga ccgtcgacca tcaggttgta ctcgaaaccg aactgttcaa
     1141 gtacggactc aacctttcgc acacgtttta cacgttggcg cgcatcgtcg cacccgtgtt
     1201 gattcgctgg ggcactgaag aacaaaaggc cgaattcttg ccggcgatta cccgggcgca
     1261 aatttgcgtc tggcaggtgc tcaccgaacc gcatggcggc agtgatattg cgaactgtca
     1321 gaccaaagcg atccgcgacg gtgatcacta tgtcgtaaac ggtcagaaag tcatggtcgg
     1381 gcatcatttg ccgccggatt atctctggac tctggtctgc accaatcccc agggcaagcg
     1441 ccacgagaac ctcgcctggc tgcacattcc cgcgaacctg tccggcatca cgattcagca
     1501 tatgaatatg atgatgggca tcaagaacgc cgtatttttc gataacgtgc gggtgccggc
     1561 gaaatatctc atcggtggcg aaaacaatgg ctggaaagtc gccagcacgc atatggaact
     1621 cgaacatggc ggcgacggcc gcatcgccgc cgatccattg atcgagcggg tggccgagta
     1681 ctgccgcaac actcatatcg atggcaagcc gatcatcgaa gatccgcgcg tgcgcgattt
     1741 gatcgccgag atgaccatcg agctcaacac gctgcggcgc ttcaatcagc tgatctactg
     1801 gcgtcgcttt gccaaaaagc cgcatccgta cggcggtcct caacagcggt atttccaaag
     1861 aatgctgcgg ctgcgcaatg gcgagcgctt acaagcaatt atgggcgccg atgccctgga
     1921 gcaaaatctc tcggtgcacg aagtcatcga tttcgaatac gcggtgcgca ccggccccgg
     1981 ccagctgcat ggcggcggca ccttggacac cgatcgattg atcttcgccc gtcgtctagg
     2041 gctcggccgt ccgtccaagg aaagcgcgcc ggaaacacat tagataaaaa cttgtagcgg
     2101 cgtagcatgc tgcgccgtct gtaggagaat cgaaatggat ttaggtttaa cagaagacca
     2161 agagatgttg aaaaaggtcg cggcggactt cgtcaaagcc gaggtgccgg cacatcaaat
     2221 gacccagtgg tacaaggaca agaaaatttt tcgacccgag ctggttagga aagccgccga
     2281 gctcggctgg ctgggcatga tgctgccgga agaatggggc ggcgccggtt tggcgccgat
     2341 ggattgcgcc gtggtgttcg agcagctggg ccacgggccg gtgccggggc cgttgttcac
     2401 atcgggcatc ctcagcgccc agatgatctt cgaagcgtgc actgacgcgc aacgaaagga
     2461 tctcctgccg gcactttgca gtggtaagtc aatcgtcata ccggcgatca ccgataaatc
     2521 agcctactgg ggaccggagg cggtcgaaac ccggctcagc aaaactcctg ggggctattt
     2581 gctcaacggc accaagcgct tcgtcttcga cgcggacgcc gcgaccaact ttctcagcgc
     2641 cgctcggact gaagaaggca aagtagtctt cttgctcgtc gatgccaagg cttccggcat
     2701 caccgtaact caacataccg gcttcttcgt ctcggtcttt gaagtgcgct tcaacaatgt
     2761 tgctctcagc gccgataacc tgcttggctc gagcggcgcc agctgggcga cgctggagaa
     2821 agcgctcgac aaagcgctgc cgatcttgtc ggcctatcaa gtcggagcga cccaggaagt
     2881 tttcgatatg acttgcgaat acaccaattc gcgcgtcgtt tttggccagc cgataggccg
     2941 tttccagcgt gtccaggacc attgcgtcga tatttcgatc tgtctggaca cggcgcgctg
     3001 ggcgacatac gaagcgatct ggcgcatcga gaacggcatg gacgcccgcg ccggcgtgca
     3061 cgaggccaag gccaccgcca gcgatggcta ctatcacggc accaattacg cacatatggt
     3121 ttgggcgggg cccggcacag actacagtca cccgatgatg gcgcacagcg tgctcgccca
     3181 tactttgtat caatatcttg gcacgcccgg acaccataaa cgtttgatgg tagacgcgtt
     3241 gtatccgcgg gcttagaact cactggtcga ggatggaaaa ttgagcatgg acgatcgcca
     3301 tctttaattc ttgccatcct cgattttcta tatccgatcc tcgagggcgt aggagctttg
     3361 aggccaagca tgaaatttac tttacattcc ttcgcgccac tcgttctgct catgctttcg
     3421 tgcaccatgc tccaggcgca atctcagtcc gccctgctcg atggcgccaa gaaggaaggc
     3481 aacctca
//
LOCUS       DATLCH010000356        13016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7982016, whole genome shotgun sequence.
ACCESSION   DATLCH010000356 DATLCH010000000
VERSION     DATLCH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13016
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7982016"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1885
                     /locus_tag="VMZ02_10510"
                     /pseudo
     CDS             113..1885
                     /locus_tag="VMZ02_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
     gene            1897..2208
                     /locus_tag="VMZ02_10515"
     CDS             1897..2208
                     /locus_tag="VMZ02_10515"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72411.1"
                     /translation="MARGHHDVGGLPGAGEINQAEHEMSDWEILADAVNQALGARGIK
                     RTDEMRRAREEMDSAAYRDMSYYERWIASIEAILIEKNILTKDEIDQKLAAFESRWGE
                     P"
     gene            2205..2498
                     /locus_tag="VMZ02_10520"
     CDS             2205..2498
                     /locus_tag="VMZ02_10520"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HUR72412.1"
                     /translation="MSSATGRFNTGDRVKVKFEDRPGHLRTPWYIRGKNGWIERVYGD
                     FLNPELLAFGKNGLPKKTLYLVAFEHSEVFGGSHGSKDKLLVDIYDHWLEPLK"
     gene            2508..3125
                     /gene="nthA"
                     /locus_tag="VMZ02_10525"
     CDS             2508..3125
                     /gene="nthA"
                     /locus_tag="VMZ02_10525"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HUR72413.1"
                     /translation="MSDDHAHDHPKHELSYYAKRIYAMRDLLVEKGVISEKDIQCQIE
                     YQEARTPANGAKLVAKAWVDTEFKARFIADAKAACAEIGIDATAINEFVVLENTPKVR
                     HMVVCTLCSCYPRPILGRPPDWYKSFNYRQRSVADPRGVMREFGLELPDDVEVRVHDS
                     TADIRYIVMPLRPAGTDGWSEDDLAKLVTRDSMIGVMDALPSLPD"
     gene            3271..3771
                     /locus_tag="VMZ02_10530"
     CDS             3271..3771
                     /locus_tag="VMZ02_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72414.1"
                     /translation="MITKPRPFRGAKRATFEFAMALRSIGQDLERRGLKTFDIRFDGR
                     EFIARCGYQMPPAATPVEIKYLPADIEELNQAGGDNRGTAAAPKDFLNHPQIFRTIGG
                     FLDRSEARLVRLANSDRRESTVVVEYLSREGELIVADRAGAEIYDMCVSMYKQRGKLT
                     GTGGRR"
     gene            3826..4347
                     /locus_tag="VMZ02_10535"
     CDS             3826..4347
                     /locus_tag="VMZ02_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HUR72415.1"
                     /translation="MDTSVDYYSVLGIKANATMDDIKRAYRQMVFRFHPDRNPDNPEA
                     VDKFNQVRDAYSVLSDALKRRVYDSIKHPAGAEESEEQRAEETPNEKEQQTSNGNGHG
                     TASGFDFKQGFKQTVDPEPKCPSCSTVGIDHIVSRKGGSGSARGKHFVLSPFHVVLCN
                     ACGHVYGVTGNSN"
     gene            complement(4391..5152)
                     /locus_tag="VMZ02_10540"
     CDS             complement(4391..5152)
                     /locus_tag="VMZ02_10540"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HUR72416.1"
                     /translation="MRLKDKVTIITGAAHGIGKAYARRFADEGAHVVIADIDAAGGEA
                     VANAILAAGGSAWARTTDVRSLADLQGLMQETVKKFGRIDVLLNNAAIYVTQNLWKGA
                     VEDLQIDEWDRVIEVNLKGVFLCSKAVIPIMKQQRSGKIINIASGTFFSGSGNMPHYT
                     TAKGGVVALTRVMARQLGPYGINVNCMTPGSTMSEENISQDVLKRREGSIDKRAFRRV
                     ETPADIVGTALFLASSDSDFITGQLLVVEGGGVMH"
     gene            complement(5201..6070)
                     /gene="hemQ"
                     /locus_tag="VMZ02_10545"
     CDS             complement(5201..6070)
                     /gene="hemQ"
                     /locus_tag="VMZ02_10545"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF008913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HUR72417.1"
                     /translation="MEERSTADQTTALPPTPLTLDGAYLLHQMFRVRWPAWKALGHTQ
                     QRHTLERSTTLFSALTANPQASTGLFSMIGHKGDLMVVHFRRTLDDLNDAELAIANSE
                     LYEYLEPTTSYLSVVELGLYEASTKLYGELTVKGLKPGTAEWNREIETELSKQRESSA
                     ARLWPEIPARRYLCFYPMNKHRGELKNWFSESIAERQRMMRDHGMIGRHYAGRVTQII
                     SGSIGFDNWEWGVDLFADDPLVFKKLVYEMRFDEASAVYGLFGDFFVGLRFPISQLEV
                     FLAGKTPSLASLP"
     gene            complement(6091..6738)
                     /locus_tag="VMZ02_10550"
     CDS             complement(6091..6738)
                     /locus_tag="VMZ02_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HUR72418.1"
                     /translation="MGTIEALSLAMGGAWASGINLYATVAALGLARRMELIQLPSNLE
                     VLSHPAVIVIACIMYLIEFFADKVPYIDSGWDALHTFIRVPAGALLAARALGDMHPAL
                     ELAAILAGGSIAFAAHGTKATTRLAINASPEPFTNWTASVTEDAAVLGGIWLIFNHPM
                     LMIVFVLAFVGLGVWIMPKVFRLAKRGLQRLRDRMRGVKPDQASTTGSPPAIVNS"
     gene            complement(6836..8266)
                     /locus_tag="VMZ02_10555"
     CDS             complement(6836..8266)
                     /locus_tag="VMZ02_10555"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR72419.1"
                     /translation="MADVTDMRGYIDVLKEIGELREFDGVDLNLEVGALTERAAEKEG
                     DALLFNNFKNYPSGFRVITNVFRTCKRTGPAMGIAAQNGIDFLYEWRKKLAAYKPVPV
                     QHVEGGPVMENQMVDEQVDLYKFPTPKWHDLDGGPFIGTGCGVITKDLDTGKVNVGTY
                     RVMVQDKNHVSVKMNMGKHGRLAFERARDAGKALPIAITLGQGPAVFLAAQMPLPPDV
                     NEFEFAGYLQGAPVPVARGAFTGLPIPANAEIVLEGELPPMKDADMPKEGPFGEWPGY
                     FTDENVGETPVMTVKRIYYRNNPILLGAPPLKPPASYLPMPLGAATLWEQLEKAGIPE
                     VKGVWGFVYGGQPGPFTVVSIKQRYAGHAKQALLVAAGARAGAYGGKFVVVVDDDIDI
                     TNPGEVIWAIATRCYVRGGIDVIKDVWASVCEPAMPPEERSPRGYTSDRVLIDACRPY
                     KWMDQFPAVNAFEKDFKDKIADKFKL"
     gene            8409..9686
                     /locus_tag="VMZ02_10560"
     CDS             8409..9686
                     /locus_tag="VMZ02_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72420.1"
                     /translation="MQFPFFYGWIIVAIAMVAGFVSSGVSNVTMAVVLKPISEDLGWS
                     RSVTAAAITLGSFGGGMLSPLFGPLADRFGPRVLMPVGGILVGLLAIGVSQSSQPWQF
                     YATFIPARALTEFLLCGVVPYTAVANWFHAKRPRAMGLVAMATPLGSAATTLIFQLLV
                     SHHGWRSAFLALGLAFLILVVVPAAIFLRRQPEDIGMYPDGIAPCALSDDSATLASEI
                     PATGETSWSRSEATRTPALWLLVVSVFLASLGTGGIAFHTIAYFTDVKISPPAAAGAL
                     SVMALAGAFGNGIWGALAERLSPRNLSVTTTALSAASVALLMQVNSPLTAYLFAVLFG
                     ICARGGTAVLTQILLAHYFGRRSFGAISGVLEPFHKGGLGVGALIAGIAFDWSGNYRP
                     VFLLFVVSYLLSATLVFFARRPKLGRKIAGFEV"
     gene            9676..11085
                     /locus_tag="VMZ02_10565"
     CDS             9676..11085
                     /locus_tag="VMZ02_10565"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUR72421.1"
                     /translation="MKFKLAAARSQLTVRLLDFEASSISLVKMIRLRRFTLLVLVLSS
                     LSPPVSFAAQVQEFVLDNGLKVLLLEDHKSPAVTFQVWYRVGGRNEKDGKSGLAHFLE
                     HMIFKGTPTLGPEEYSRVIAKNGGRSNAFTTADMTVYFATMSRDKIHIQLELEADLMA
                     NALLGEAYFEPEKKVIQEERRLRTDDNPGSALYEVAASVAYTIHPYRRPVVGWMEDIQ
                     NLTRQDLVDFYKEYYAPNNAVVVMVGDFNSEEMLAKIKSAYGKIPRRPDPPKLSVAEP
                     PQRGEKRVTLKKDAELPILLMLHHVPNLHSPDTFALDVLSVVLAGGKSSRLYQELVYQ
                     KRLVRGIDADYSGVAMDPTAFAVSAQLLPGAELAKVENEIVRLLDKTKVELVSERELQ
                     KAKNQIEAAFVFAQDSIFGQAMKIGYYEMVGNWRLVDSYLESMRKVTREDLRRVAQKY
                     LQQDNRTVATLIPTKAKLP"
     gene            11082..12401
                     /locus_tag="VMZ02_10570"
     CDS             11082..12401
                     /locus_tag="VMZ02_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HUR72422.1"
                     /translation="MTRKLIGCFLFAMLAHGLPAHGAMAVKRTVLSNGLVLLTSEQRA
                     LPMVSIEMLIDAGARHESAEQAGLAHLTSKLLTEGTAKRNAQQISETLDFIGAGLDTG
                     CGTDLATISITILKKDLLVGLQLLAEILASTSFPLVEMDRQKQAIIASIKAKEEDPGT
                     LAGEAFAAALFPGSPYGRPVEGTEASVKRLDRARVQDFFRRYYRPNRTIMSVVGDVSE
                     QEISVALNQVFTTWTKGEASKPPVAPALNDKAQVLRVNKDLTQANIVLGHGGVPRSHP
                     DYYGIQVMNYILGGGGFSSRAMESIRNERGLAYSVYSYFGAEKSYGIFQFVMQTKNET
                     AAQAIGLAQDEIRRMRDELVSEVELNDAKDYLVGSFPLRFDTNRKVAAFLGQVEYYGL
                     GLDYPERYGDLIRKVSRADVQRVAREHLFPEKLITVMVGNLQKMKVQ"
     gene            12583..12960
                     /locus_tag="VMZ02_10575"
     CDS             12583..12960
                     /locus_tag="VMZ02_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR72423.1"
                     /translation="MKKILIIEDHADMRELLTWQVELMGFTALTAKHGSEGLERAQSE
                     TPDLIILDIMMPGMDGWQAARELKSNTVTKRIPILAATALFRDTDLKTCMDSGCNGYI
                     VKPFTFQELQGKVRELLPAALPN"
BASE COUNT         2838 a         3490 c         3708 g         2980 t
ORIGIN      
        1 cgaacgtgaa cgatatcgcc accttgactg gcgtcgtgag acggattgga aggttctctg
       61 ttcgatcgta tgacgtctcc tccagcggtt taggaaagga actttataac ccatgactaa
      121 cgtaacagga tcagtgccgg caaatccgca tattcaaact tgggtgaaaa gtgtcgcggc
      181 aatgtgccag ccggacaatg ttttttggtg cgatggttcg gaagaagaaa aagaactgct
      241 gaccaagatc gcggtacaaa gcggcgattt gttgccgctc aaccaaaaag ccgcaccggg
      301 ctgttacctg caccgcagcg cactcaatga cgtcgcccgc actgaaaatc tgacgtttgt
      361 gtgtaccgaa cagcaggaag aggctggccc taacaataac tggatggcgc cggcggaatc
      421 ctacgacaaa ctcggcaaaa ttttcgccgg ctctatgcgt ggacgcacgc tttacgttat
      481 tccctttctc atggggccac agggttcgtc gttcagcaag gtcggtgtgc aagttaccga
      541 cagtgtttac gtcgtcctca atatgcgttt gatgacccgt atgggcaaaa tcgcattgga
      601 ccatctcggt aactccgatg attttacccg ttgccttcat tcgaaggccg acctcgatat
      661 tgagcgccgc ttcatctgcc attttcctca ggacaataca gtttggagtg tcggctcggg
      721 gtacggcggc aacgcgctgc tcgcgaaaaa atgtttatca ctgcgcatcg cgagcaaact
      781 cggtcaacgg gaaggctgga tggccgaaca tatgctgatc gtcggagttg agaatcccga
      841 cggtgagatt acttatgttt gcggcgcgtt cccgagcgct tgcggtaaga cgaacatggc
      901 gatgctggtg ccgccggcga gtatgaaggg ttggaagatt cacaccgtcg gcgatgacat
      961 ttcttggctg cgcgtcggtc aagacggtag actctgggcg attaatcccg aagcaggatt
     1021 tttcggcgtc gctcccggta ccggctccaa taccaacccc aacgcggtga aaaccattaa
     1081 aagggatacc atcttcacca acgtggcgct ccgtgacgac gggacggtct ggtgggaagg
     1141 gcacgatgat ccggttccgg ataacgcgca ggattggcgc ggcaaaccat gggacccgga
     1201 aggtggcagt ccggcggcgc atcctaacgc gcgcttcacg gtttcgatca agcaatgccc
     1261 gacctagtcg cctgagtggg aaaaacctga aggggtgccg atcagcgcaa tccttttcgg
     1321 tgcgcgtcgg cagaaactcg tcccattggt ttaccaatca tttaactggc aacacggcac
     1381 ttttctcggc gcgaccttgg catctgaaac taccgcggcg gctaccggcg ctgtaggtgt
     1441 ggtgcgacgc gatcccatgg cgatgctgcc attctgcggc tacaacatgg gcgattattg
     1501 ggcacattgg ttggccatgg gaaagaaggc gagcaatccg cccaaggttt tccgtgtcaa
     1561 ttggtttcaa cgcaacgagc gcggtcgttt tttgtggccg ggattcggcg aaaatctgcg
     1621 ggtactgcgc tgggtggtcg aacggtcgaa aggcggcggt caggccgatg agacgcctat
     1681 cggattcgtg ccaaaagcct cgagcatcac cggagatggt ctcgatatct ccagagccga
     1741 tgtcgatcag ttgctgtcag ttgatcgcga aggctggaag aataatctga aaagccagag
     1801 tgatttcttc aataccttcg gcgatcattt gccggcgggc atgaaggaag agcatggcgc
     1861 gttggccaac cggctcaagg gctagaaggg gatcatatgg cgcgtggaca tcacgatgtc
     1921 ggtggcttgc ccggggcagg ggagatcaat caggcggagc atgagatgtc cgattgggaa
     1981 attctcgcgg acgccgtcaa ccaggcgctg ggtgcacgcg gcatcaaacg caccgacgag
     2041 atgcgccggg cccgcgagga aatggattcc gcagcgtacc gcgacatgag ttactacgag
     2101 cgttggatcg ccagtatcga agccattttg atcgaaaaaa atattcttac caaagacgag
     2161 atcgaccaga agctcgccgc attcgaatct aggtggggcg agccgtgagc tccgccaccg
     2221 gtagatttaa taccggcgat cgagtgaaag tgaaattcga agatcgcccg ggtcacctgc
     2281 gcacaccctg gtacattcgc ggcaagaacg gttggatcga acgggtctat ggagattttc
     2341 tcaatcctga attactcgcg tttggcaaga acggcttgcc caagaagaca ctctatttgg
     2401 ttgctttcga gcacagtgaa gtgtttggcg ggtcgcacgg ctccaaagac aagcttcttg
     2461 tcgatatcta cgatcactgg ctcgagccgt tgaaatgagg agatgttatg agtgacgacc
     2521 atgcacatga tcaccccaag catgaattga gctattacgc taaacggatc tatgcaatgc
     2581 gcgatctatt ggtagaaaag ggtgtgatca gcgaaaaaga cattcagtgc caaatcgaat
     2641 atcaggaggc gcgcacaccg gccaacggcg ccaagctggt cgccaaggcg tgggtcgata
     2701 cagagttcaa agcacgtttc attgccgacg cgaaagccgc ctgcgcagaa ataggcatcg
     2761 acgccacggc aatcaatgag tttgtcgttc tggaaaacac gccgaaagtg cgccacatgg
     2821 tggtgtgtac cttgtgttcc tgctatccgc ggccgatcct tggccggccg ccggattggt
     2881 acaaaagttt caattatcgc cagcggtcgg tagcggaccc gcgcggcgtc atgcgcgagt
     2941 tcggtctgga actccccgac gatgtcgaag tgcgggtgca tgatagcacc gccgatatcc
     3001 gctatatcgt catgccattg cgtcctgcgg gcaccgacgg ctggagcgag gatgatctgg
     3061 cgaaactggt gacgcgtgac agcatgattg gtgtcatgga tgcgctaccg agtttgcctg
     3121 attaacacat tctttccgca actaccgcca atttccctgt cgtacattag aatcggcgga
     3181 atcagccatt tgggcactgc tgccgatctt ttttgttgat tttttgccct actgttctta
     3241 ccattcactc aagaagtcct tcagggaatt atgattacca aaccgcgccc tttcagagga
     3301 gccaaacgtg cgactttcga gtttgccatg gcgctgcgca gtatcgggca agatttggaa
     3361 cgccgagggt taaagacatt cgatatccgc ttcgacggtc gcgagtttat agctcgttgc
     3421 ggttatcaaa tgccgccggc ggcaacacca gtggaaatca agtatcttcc ggcggacatc
     3481 gaagaattga atcaggccgg tggcgacaat cgcggcaccg ccgcggcgcc gaaggatttc
     3541 ctcaatcatc cgcagatttt tcgtaccatt ggcggttttc tcgatcgcag cgaagcacgg
     3601 ttggtgcggt tggccaacag cgaccgtcgc gagtcgaccg tcgtcgtcga atatctaagc
     3661 cgtgagggtg agctcatcgt cgccgatcgc gccggcgcgg agatttacga catgtgcgtc
     3721 agcatgtaca aacaacgcgg caagttgacg ggtaccggcg gccgccgcta actgcgctcg
     3781 ttccaacctg ctgatgtttg aaatgactcc cgaaagtctg tgaaagtgga tacctcggta
     3841 gattattatt ccgtactcgg catcaaggcc aacgctacga tggacgacat caagcgcgcc
     3901 taccgtcaga tggttttccg ttttcatccc gaccgcaacc cggacaatcc ggaagccgtc
     3961 gacaaattca atcaagtgcg cgatgcctac tcagtgctat ccgatgcact gaaacgccgt
     4021 gtatatgata gcatcaagca tccggccgga gccgaggaga gcgaagagca aagggcagaa
     4081 gaaacgccga acgaaaaaga acagcagacg tccaacggca atggtcatgg cacggcgagc
     4141 ggttttgatt tcaagcaagg attcaaacag acggttgatc ctgaacccaa gtgcccgtcc
     4201 tgttcgaccg tcggaatcga tcacatcgtt tcgcgcaagg gcggatctgg cagcgcgcgc
     4261 ggcaagcact ttgtcctgtc tcccttccat gttgtgctct gcaacgcctg tggtcacgtt
     4321 tatggcgtga ctggcaattc caactgatcg ttttacgaaa cgcgaacaat tttcgtttca
     4381 ttttcacggc tcagtgcatg acgccgccgc cttcgaccac cagcagctgc ccggtgatga
     4441 aatcgctgtc cgaactggcg agaaacaatg cggtaccgac aatgtcggcg ggagtttcga
     4501 cgcggcgaaa tgcgcgtttg tcgatgctgc cttcgcgccg tttaagaaca tcttgagaaa
     4561 tgttttcttc gctcatggta gagcccggtg tcatacagtt gacgttgatg ccgtacggcc
     4621 ccaactggcg cgccattacg cgggtcaacg cgacgacacc gcctttggcg gtcgtgtaat
     4681 gcggcatgtt gccggagccg ctaaaaaaag ttccagaggc gatattaata atcttgcccg
     4741 agcgctgctg tttcatgatc ggaatcaccg ctttgctgca taagaacaca cccttcaaat
     4801 tgacttcgat gacgcggtcc cattcgtcga tttgcaaatc ttccacagcg cctttccaga
     4861 gattctgcgt cacgtagatc gccgcattat tgagcaggac gtcgatacgg ccgaattttt
     4921 tcaccgtttc ctgcatgaga ccctgaaggt ctgccaaact acgcacgtcg gtggtgcgcg
     4981 cccaggccga cccgccggct gcgaggatcg cgttcgccac agcttcgcct ccggctgcat
     5041 cgatgtcggc gatcaccaca tgagcacctt catcggcgaa gcggcgcgca tacgctttgc
     5101 caatgccgtg ggccgctcct gtgatgattg taactttatc tttgagtcgc atagacctcc
     5161 gttgccaagc cgtgccgctc gcccgcaatt cgcataacag ttagggcaag ctagccaagc
     5221 tgggtgtctt accggcaaga aagacttcga gctggctgat cggaaaacgc aggccgacga
     5281 agaagtcgcc gaacagacca tagactgcgc tcgcttcgtc gaagcgcatt tcgtagacca
     5341 gcttcttgaa aaccaacgga tcgtcggcaa acagatcgac gccccactcc caattatcga
     5401 agccgatgga cccggaaatg atctgggtga cgcgcccggc gtagtggcgc ccgatcattc
     5461 catggtcacg catcatgcgc tggcgttcgg cgatggattc actaaaccaa ttctttagtt
     5521 cgccgcgatg tttattcatc gggtaaaaac aaagataacg ccgcgccggg atttccggcc
     5581 agagacgagc cgcggagctt tctcgctgct tggataattc cgtttcgatt tcgcggttcc
     5641 attcagcggt gccgggctta agacctttga cagtgagctc gccatagagc ttggtcgaag
     5701 cctcgtacag gccgagctcg accaccgaga gataagacgt ggttggttcg aggtattcgt
     5761 acagctcgct attggcaatc gccaattccg cgtcattcag gtcatcgagg gttcgccgga
     5821 aatggacgac catcagatcg cccttatgtc cgatcatgga aaacagcccg gtgctagcct
     5881 gcgggttcgc cgtcagtgca ctgaacagtg tcgtagatct ctccaatgta tgtctttgtt
     5941 gcgtgtgacc gagcgctttc caggccggcc agcggacgcg aaacatctga tgcagcaaat
     6001 aggcaccgtc gagggttaac ggcgtcggtg gcaaagcggt ggtttgatca gctgtcgatc
     6061 gctcttccat tgctttcggt acccttcgac tcaagagttc actatcgccg gtggcgagcc
     6121 ggtggtggac gcttgatcgg gtttaacgcc gcgcatacga tcgcgcaggc gttgcaagcc
     6181 gcgctttgcg aggcgaaaaa cttttggcat gatccagact cccaacccga caaaagctag
     6241 gacgaaaact atcatgagca tcggatgatt aaaaattaac cagatacctc ccaagacggc
     6301 ggcatcttca gtaacgctgg cggtccagtt ggtgaatggc tcgggactgg cgttgatcgc
     6361 cagtcgcgtg gtcgctttgg tgccgtgagc ggcaaatgcg attgagccgc cggcgaggat
     6421 tgccgccaac tccaaggccg gatgcatatc gccgagagcg cgcgcggcta agagcgcgcc
     6481 tgccggcacg cggatgaagg tgtgaagcgc gtcccaaccg ctgtctatgt aaggcacttt
     6541 atccgcgaaa aactcgatca aatacatgat gcaggcgata acgatcaccg cgggatgcga
     6601 cagcacttcg agattcgacg gtaactgaat tagctccatc cggcgggcaa gccccaaggc
     6661 cgccacagtg gcgtatagat tgatgcccga cgcccaggca ccgcccatgg ccaagctcaa
     6721 tgcttcgatc gttcccatct gccgactcct tttgcgccgc tagagcgctc ggttcagcta
     6781 gttcgaggtt tatgccgtcg ctatatcgcg tgttcctctc gggctgagag aatctctaca
     6841 acttaaattt gtccgcgatt ttatctttaa aatctttttc aaaggcgttg accgccggaa
     6901 actgatccat ccacttatag ggtcggcagg cgtcgatgag cacgcggtcc gaagtgtagc
     6961 cgcgcggcga gcgttcttcc ggcggcatgg ccggctcgca caccgaggcc cagacgtctt
     7021 tgattacatc gatgccgccg cgcacatagc agcgagtagc gatggcccag atgacctcgc
     7081 cgggattggt aatatcgatg tcgtcgtcta cgaccacgac aaacttgccg ccataagctc
     7141 ccgcacgcgc gccagcggcg actagcaatg cttgtttggc atgacctgcg tagcgctgct
     7201 ttatcgaaac gacggtgaat ggtcccggct ggccgccata aacaaagccc cagacgcctt
     7261 tgacctcagg gattcccgcc ttttccaact gttcccataa ggtcgcggcg cccaacggca
     7321 tcggtaaata acttgccggc ggcttgagcg gcggcgcgcc aagcaggatc ggattgttgc
     7381 gataatagat ccgcttgaca gtcatgaccg gagtctcgcc tacattttca tcagtaaaat
     7441 atcccggcca ctcgccaaag ggaccttcct tcggcatgtc cgcgtctttc atcggcggca
     7501 attcgccttc gaggacgatc tccgcgttgg ctggaatcgg cagtcccgtg aaagcgccgc
     7561 gcgccacggg cacgggcgcg ccttggagat aaccggcaaa ctcaaactca ttgacatccg
     7621 gcggcagcgg catttgcgcc gcgagaaaaa ccgccgggcc ctggcccaaa gtgatcgcaa
     7681 tcggtaaagc tttgccggca tcgcgagcgc gctcgaaggc gagccggcca tgtttgccca
     7741 tattcatttt caccgagacg tgatttttat cctgcaccat aactcgatag gtacccacat
     7801 tgactttgcc ggtatccaaa tctttggtga tgacgccgca gccggtgccg atgaacggcc
     7861 cgccgtcgag atcatgccat ttgggagtgg gaaatttgta gaggtcgact tgctcatcga
     7921 ccatctggtt ttccatcacc ggtccgcctt cgacgtgctg taccggcacg ggtttgtaag
     7981 ccgccaactt ttttcgccat tcgtaaagaa aatcgatgcc gttttgcgcg gcaattccca
     8041 tcgccggacc agtgcgcttg caagtgcgaa aaacattggt gatgacgcga aatccggatg
     8101 gatagttttt gaaattgtta aacagcagcg cgtcgccttc tttttccgcc gcccgctcgg
     8161 tgagcgcgcc gacttccaag tttaagtcga cgccgtcaaa ttcgcgcaac tcgccgatct
     8221 ctttgaggac gtcgatgtag ccccgcatgt ccgtaacatc agccataatt tcctcctaaa
     8281 ttggcgcaat agtatggcgg cgatggaggg gagtcaatcg actgatcgtc ggtctgtctc
     8341 aacggtaatc tgcgatgcga atgttttcgc tccggtattt gttgcgaaac tagtgtaaga
     8401 aggactttgt gcagtttcct ttcttttacg gctggatcat cgttgccata gcaatggtcg
     8461 ccggatttgt ttcttccggc gtgagcaatg tgaccatggc tgttgtattg aagccgatca
     8521 gtgaagatct tggctggagc cggagtgtta ccgcggcggc aattacttta ggttctttcg
     8581 gcggcggcat gttgtcgcct ctgttcggac cgctggcaga tcgttttggg ccgcgggtgc
     8641 tgatgccggt gggcggtatt ctcgttgggc tcttggccat cggcgtgagt caaagttctc
     8701 agccctggca gttttatgcg acgtttattc cggcgcgcgc tctgacggaa tttttgctct
     8761 gcggagtcgt gccgtatacc gctgtcgcca actggtttca tgccaagcgg ccgcgcgcca
     8821 tgggcttggt ggcgatggcg acgccattgg ggtcggcggc aacaacgctg atttttcagt
     8881 tgctggtcag tcatcatggt tggcgcagcg cgtttttagc cttgggtttg gcatttctta
     8941 ttctcgtcgt cgtgccggcg gcaattttcc tgcgccggca accagaagat atcggcatgt
     9001 atcccgatgg catcgcgcct tgtgcgcttt ccgacgatag cgctacattg gcttccgaga
     9061 ttccagccac aggcgagacg agttggtctc gatccgaagc gacgcgcacc ccagcgctgt
     9121 ggttgctcgt ggtgagtgtg tttcttgctt cgctcggcac cggcggcatc gcctttcata
     9181 ccattgccta ttttacggat gtcaaaattt caccgccggc ggccgccggc gcgttgagtg
     9241 tgatggcgct tgcgggggcg tttggcaacg gcatctgggg cgcactggca gaacgactct
     9301 cgccgcgaaa tttgagtgtg acgacgacgg cgttatcggc ggcgtcggtg gctttattga
     9361 tgcaggtcaa ttcgccgctc acagcctatc tgttcgccgt cctgttcggc atttgcgcgc
     9421 gcggcggcac ggctgtgttg acacagattt tattggcgca ctatttcggc cggcgctcct
     9481 ttggcgcgat cagcggcgtg cttgagccgt tccacaaagg cgggcttggc gttggcgcct
     9541 taatcgctgg tattgccttc gattggagtg gcaactatcg cccggtgttc ctattgtttg
     9601 tggtcagcta tctattgtcg gctactctgg tatttttcgc ccgacgtcca aagctcggcc
     9661 gcaaaatagc agggtttgaa gtttaaatta gccgccgcgc gatcacagct gaccgtccgt
     9721 ttgcttgact ttgaggcttc atcgattagc ttggtaaaga tgattcggct tcggcgattt
     9781 acactgcttg tccttgttct ctccagcctt tcaccccctg tctcgtttgc cgcgcaagtt
     9841 caagaatttg ttctcgacaa cggtttgaaa gtactgcttt tggaagatca caagagcccg
     9901 gcagtgacct ttcaggtgtg gtatcgggtc ggcgggcgta atgaaaagga cggcaagagc
     9961 ggtttggcgc atttccttga gcatatgatc ttcaagggca cgccgacgct cggaccggaa
    10021 gagtactcgc gggtcatcgc caagaatggc ggtcgttcca atgcgtttac gacagcggac
    10081 atgaccgtct atttcgcgac gatgagccgc gacaaaattc atattcaact cgagctggaa
    10141 gctgacctca tggccaatgc gctgctcggc gaagcttatt tcgagccgga gaagaaggtt
    10201 atccaagaag agcgccgctt acgcaccgac gataatccgg gctcagcctt atacgaggtt
    10261 gctgcttccg tggcttacac gattcatcct tatcgtcggc cggtggtcgg ctggatggaa
    10321 gatattcaaa acctcacccg tcaggacttg gtcgattttt acaaagaata ttatgcgccc
    10381 aataatgccg tggtcgtcat ggtgggtgat ttcaactcgg aagaaatgct cgccaagatc
    10441 aaatctgcct atggcaaaat tccgcgccgc cccgacccgc cgaagctgtc ggtggccgag
    10501 ccgccccaac gcggcgaaaa gcgtgtgacg ctgaagaaag atgccgagct gccaatactg
    10561 ctaatgcttc atcacgtgcc caatctgcat agcccggata cttttgcgtt ggatgttttg
    10621 tcggtggtgc tggccggcgg caagagttca cgcttgtatc aggaactggt ttatcaaaag
    10681 cgcctggtgc gcggtatcga tgccgattac agcggggtgg cgatggatcc caccgcgttt
    10741 gctgtttccg cccagctttt gcccggcgcg gagctggcca aagtcgagaa cgaaatcgta
    10801 cgcttacttg acaagaccaa ggtcgagctg gtgagcgaac gcgaattgca gaaagccaag
    10861 aaccagatcg aagccgcgtt tgtcttcgcc caagactcga tcttcgggca agcgatgaaa
    10921 atcggatatt atgagatggt cggtaactgg cgcttggtcg atagttatct cgagagtatg
    10981 cgtaaagtca cccgggagga tctccgccgt gtggcgcaga aatatcttca acaagacaac
    11041 cggacggtgg ctaccttgat tccgacaaaa gcgaaactgc catgacgcgt aaattgatcg
    11101 gctgtttttt atttgccatg ttggcgcacg ggttgccagc gcacggcgcc atggcggtga
    11161 aacgcacggt gctcagcaat ggattggttt tgctcacctc ggagcaacgc gcattgccga
    11221 tggtgtcgat cgaaatgttg atcgatgccg gggcgcgcca tgagagcgcg gagcaggctg
    11281 gtttggcgca tttaacttca aagctgttga ccgagggcac tgcgaagcgc aacgctcagc
    11341 agatcagcga gacgctggat tttattggcg cgggtctcga caccggttgc ggcaccgatc
    11401 tggcgacaat cagcattact atcctaaaaa aagatttgtt ggttgggttg caattgcttg
    11461 ccgagattct cgccagtacg agctttccac tggtcgaaat ggaccgccag aagcaggcga
    11521 tcatcgcttc gatcaaagct aaagaagaag accccggtac cttggccggt gaagcttttg
    11581 ccgcggcatt gtttcccggc agtccctacg gccggccggt ggaaggaacg gaggcttcgg
    11641 taaagcggtt ggatcgcgcg cgtgtgcaag atttttttcg gcgctactac cggccgaatc
    11701 gaacaatcat gtcggtggtc ggtgatgttt ccgagcagga gattagcgtc gcgctcaatc
    11761 aagtgttcac gacctggacc aaaggcgaag caagtaaacc gccggtcgcg ccggcgctga
    11821 acgacaaggc gcaggtattg cgggtgaaca aggatctgac acaggcgaat atcgttctcg
    11881 gacatggcgg tgtgccgcgc agtcatcccg attactacgg tatccaagtt atgaactaca
    11941 ttctcggcgg cggcggcttt tcatcgcggg cgatggaatc gatccgcaac gagcgtggct
    12001 tggcttattc ggtctacagc tatttcggtg cggagaaaag ctacggaatt tttcaatttg
    12061 tcatgcagac caagaacgag accgcagcgc aggcgatagg ccttgcgcag gacgagatca
    12121 ggcgcatgcg cgatgaactg gtcagcgagg tcgaattgaa cgatgctaaa gattacctag
    12181 tcggcagctt tcctttacgt tttgacacca accgcaaggt tgctgcgttt ttgggtcagg
    12241 tggaatatta cggattggga ctcgactatc ccgagcgcta cggcgattta atccgcaagg
    12301 tcagccgcgc cgacgtgcag cgggtcgccc gcgaacacct ttttcccgaa aaattgatca
    12361 cggtcatggt cggtaatctg cagaagatga aggtgcaata gacctcgagt aggcaatgcg
    12421 tgacagcaaa taaaatttgc ggcagttgac cgaatcctcc tcgttataaa atttacccac
    12481 cggcaagatt ctcggctatt ttcgtaacgt tgcgctgctt cctgagtcgc ttcgcggcag
    12541 ggttcgtgct aagttacagt gctcatattc gcctcaacgg ctatgaagaa aatcttgatc
    12601 attgaagacc atgcggacat gcgtgaattg ctcacctggc aggtcgagtt gatgggtttc
    12661 acggcgctta ccgcgaagca tggtagcgaa ggactggaaa gagcccaaag cgaaactcct
    12721 gatctcatta tcttggatat catgatgccg ggcatggatg gttggcaggc cgcacgagag
    12781 ctcaaaagca ataccgtgac caagagaatt cctatcctcg ccgccactgc attgtttcgc
    12841 gataccgatc tcaaaacctg tatggactct ggctgcaatg gctacatcgt caaaccgttt
    12901 acttttcaag agctgcaagg gaaggtaagg gaactgcttc ccgccgcctt gcccaactaa
    12961 cagttgaccc cgccggctaa aatcggccgg tttgccgtag tatcactgtt cgattc
//
LOCUS       DATLCH010000357         6251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3708328, whole genome shotgun sequence.
ACCESSION   DATLCH010000357 DATLCH010000000
VERSION     DATLCH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3708328"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VMZ02_10580"
     CDS             complement(<1..679)
                     /locus_tag="VMZ02_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72424.1"
                     /translation="MSNTPAARQHSDSARFFYGWYIVGVGILVNIAGTFSFSSTLSIF
                     LKPITEELGVTRGAFSLIRTFEIGVAALIIPMLGPWIDRHGGRGVLVLGVLMEGAGLL
                     LSSLVESFWQFVVVRCSLVIAGEALLGSLVINVTIAQWFVRKRGRAMGIANLGTGIAK
                     LSIPIAAAWLFVLVGWRNTWAIFGVIAPLLVVAPALLFVRRQPEDLGLHPDGDPPPAT
                     DSQAGESE"
     gene            1089..2165
                     /locus_tag="VMZ02_10585"
     CDS             1089..2165
                     /locus_tag="VMZ02_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HUR72425.1"
                     /translation="MLILSNEDIENILPVGACLDVLEEAYRDLGNGMAATVPRYDVFS
                     PTKPNEFYEYKTMSGVLPSRGIAALRLNSSVVKWYEKAGGVRKDKLPVAGGDRYVGLI
                     MLFSTETGEPLAIFPDGYVQKLRVAGASAIAARYLARHDSTTMALLGSGWQASAHLVT
                     MAMIRNLKQVRIFSPTPESRRKFVQDWRAKISAELIDCASAAEAIKDADMVICTTNSI
                     TALFSGEILQSGMHVSCVKPCELDALTYKRADPLIIHWREAKPFQITIGVDPQSIPDV
                     AEGWHHPITREDAAIWDLPTLSQLVNGQHPGRVKEDAISCFCNNVGLGLQFAAVGSEV
                     LARAREARVGREIPTDWFLEDVHP"
     gene            complement(2698..3402)
                     /locus_tag="VMZ02_10590"
     CDS             complement(2698..3402)
                     /locus_tag="VMZ02_10590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HUR72426.1"
                     /translation="MTTDPGEIADLIAALDHPDKPTIRAAVDKLIGLAGSSAELTDAL
                     NERLQAAHHKNYWPVAYVLGHQSQPSGVVVRTLLSALDHREPDIRWANGLLLVRIAQR
                     EPAVVPLLVSLCETGSSNQKRMALYCLRDLALNDAETRAAMLKRLDDDEATVRVAAIT
                     SMKVRTDVDATVRQKLLTTYLHDTDDRVKNAVAVTLASLGSPAEEFLTALREASTCAN
                     AQIKKSADAALKILQK"
     gene            complement(3433..4638)
                     /locus_tag="VMZ02_10595"
     CDS             complement(3433..4638)
                     /locus_tag="VMZ02_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HUR72427.1"
                     /translation="MTTTMFEAKEKPQNKSSGSAPQPIHLKPNQAILLYAASEGDANM
                     LYATGFFVPDPFIFFEHKNRRHIVMSDLEIDRANKQAHADRVVSLSLYQKKLRKLGKT
                     PAGIIDIVDLIFRERGIRSLIVPANFSALLLDQLRAKGYSVQIKRDPFWPQRETKNTL
                     EVKQITESLRIARLGLESGIRMLRRTKPKRDGYLYLNGTRLTSEMLKTAVNTTIMAQG
                     WLPSHTIISSGNQCVDPHHEGTGPIRANTSIIFDIFPRSQHNGYFGDLSRTVVRGRAS
                     EKLKEIYATVQAGQQIGYEQIRDGVNGKEVHQNILNMFAARGFPTGRINGRMQGFFHG
                     TGHGLGLDIHEPPRIAPVDAVLRTGHVVTVEPGLYYIGVGGVRLEDVVVVKPKGNRNL
                     TDCPQFLEI"
     gene            4944..>6251
                     /locus_tag="VMZ02_10600"
     CDS             4944..>6251
                     /locus_tag="VMZ02_10600"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HUR72428.1"
                     /translation="MTIANNLDKLRELNDKAEAGGGAERARRQHEQGKLLARERLNIL
                     LDSGSFVEMDRLRTHHSTDFGLEKQKFLGDAVVTGSGAIDGRLVFVYSQDFTVFGGSL
                     SRVVADKICKIMDMAIKNGAPLIGINDSGGARIQEGIDSLAGYGDIFQRNVMASGVVP
                     QITAIMGPCAGGAVYSPAITDFIFMVKTNSYMFITGPDVIKTVTHEEVSKEDLGGAET
                     HTAKSGVAHFAANTEKDCLLMIRELVSFLPSNNQEDPPFRPTNDDPLRRDKRLKDIIP
                     DNPNRPYDMKELIVGVVDEGYFYEVQPEFARNILIGFAHIGGRSIGIVANQPNNLAGC
                     LDIDASVKAARFIRFCDCFNIPIVTFVDVPGFLPGTEQEYHGIIRHGAKLLYAYAEAT
                     VPKVTIVTRKAYGGGYIVMASKHLRGDINLVYPTGEIAVMGPEG"
BASE COUNT         1364 a         1686 c         1809 g         1392 t
ORIGIN      
        1 gttcggattc acccgcttgg gaatctgtcg ctggcggcgg gtccccatcg ggatgcagtc
       61 ccaagtcttc aggttgccga cgcacgaaga gcagcgccgg cgcgaccacc aagagcggcg
      121 cgatgacgcc gaaaatcgcc cacgtgttac gccagccgac gaggacgaac agccacgcgg
      181 cggcaatcgg gatgctcagt tttgcgatgc cggtgccaag gttggcgatg cccatggcac
      241 ggccgcgttt gcggacaaac cattgggcaa tggtgacgtt gatgaccaat gagccgagca
      301 atgcttcgcc ggcgattacc aacgagcagc gcaccacgac gaattgccaa aagctctcaa
      361 ctaggctgga aagcagcagg cccgcgcctt ccatcaacac accaaggacg agtacgccgc
      421 gcccgccatg ccgatcgatc catggcccga gcatcggtat gatcagcgcg gccacgccga
      481 tctcgaacgt gcggatcagc gaaaacgcgc cgcgcgttac gcccaactcc tcggtgatgg
      541 gcttaagaaa tatgctcagc gtgctggaaa acgagaaggt accggcaatg ttgacgagta
      601 tgccgacgcc gacgatgtac cagccgtaga aaaaacgcgc agagtcagaa tgctgtcggg
      661 ccgcaggtgt attgctcatg gattcttgat caaccggcaa gatccctttc ttcgggagaa
      721 tgccagtcag gattatcttg tcatttcgag gtgcagcggg aagtcgtttc tactaaccga
      781 ggccgagtag atatgcacca aggccgacga ttatcgcata agtgaactga tcggccgtct
      841 ccggcgcgtt ttggctgccg aggttagaga ctgctgcgca cgtgcgctgc cctgaccgcc
      901 aacgcgaagc aaagtcgtct tcagaatggc gtcagcgagt gtgtgtcggg aaattcatcg
      961 gtctcgatca aacataatct ccgttggtct gtcaaatcag cgcgccttgc aaaaaaacac
     1021 gatgctcact aagactggct tacccagact aagcctggca ttggagcggc gaatcgcgga
     1081 acgtgcatat gctaatactt tccaacgaag atatcgaaaa tatccttccc gtgggcgcgt
     1141 gccttgacgt tttggaagaa gcttatcgcg atctcggcaa cggcatggcg gcaacggttc
     1201 cgcgctacga tgtttttagc ccgaccaagc ccaacgagtt ctacgagtat aaaactatga
     1261 gcggcgtgct gccgagccgc ggcatcgccg cgctccgact caactccagc gtcgtcaagt
     1321 ggtacgagaa agccggcggt gtgcgcaagg ataagctgcc ggtagccggc ggcgatcggt
     1381 acgtaggtct gatcatgttg ttcagcaccg agaccggcga acctttggcg atattccccg
     1441 atggttacgt gcaaaagttg cgggtcgccg gcgccagcgc cattgcagcg cgctatctcg
     1501 cacgtcacga ttccactacc atggctcttc tcggttcggg ctggcaggcg agtgctcatc
     1561 ttgtcacgat ggcgatgatt cgtaacttaa aacaagtgcg catttttagc ccgacgccgg
     1621 agtcgcgccg caaattcgtc caggactggc gtgctaagat ttctgcggaa ctgatcgact
     1681 gcgccagcgc cgcggaagcg atcaaagacg ccgatatggt gatttgcacg accaactcaa
     1741 tcacggcgct cttttcaggt gaaatcttac aatccggcat gcacgtgtcg tgcgtcaagc
     1801 cttgcgagct cgatgcgctg acctacaaac gtgccgatcc gctgatcatt cactggcgcg
     1861 aggccaaacc gttccagatc actatcggcg tcgatccgca gtcgattccc gacgtcgcag
     1921 agggctggca ccatccgatc acccgggaag acgccgcgat ctgggatctg ccgacgctct
     1981 cgcagttggt caatggccag catccagggc gagttaaaga agatgcaatc agctgttttt
     2041 gtaacaacgt cggcctcggt ttacagtttg ccgccgtcgg cagcgaagtg ctggcgcggg
     2101 cgcgtgaggc gcgagtagga cgggaaattc ccaccgattg gtttctggaa gatgtgcatc
     2161 cgtaaactgt agttcaacgt tcaagtgttc aaggttcaac gtcggacgag tagcggtgat
     2221 ataactttcg attatctttt tggatggtcg tttcacattc acccagcgaa ggccgagatc
     2281 aagcttaggt gctcgccggg cgacggtggt tgacggcgct gctgtgggtg gtgtgaagcg
     2341 ttggaccgcc ggccgagata tccgcaatga gctatgaggc gtcacctatt aaggcagtag
     2401 tgaattcgcc ggaatatata aatcttgtaa gctgtggtcc agaaaaaaca aaatttttca
     2461 gtttatcgac gtgattatta ttgacttgaa aaaatcactt tcgtaaacta ctgatcagca
     2521 gggaaaggag gtggtccaga catgattgat ttaagtactg gctgtgaggt ggctgagact
     2581 taggctttaa ggcggaaact ctgacggagg ttccggtcga aagcctggga atctccctca
     2641 gccaccgcag tggcccgctg caccggctgc ataagccgcc gagtgtggcg ggccttctta
     2701 cttttgcaat attttgagcg ccgcatcggc gctctttttg atttgagcgt tggcgcacgt
     2761 gctcgcttca cggagcgctg ttaaaaattc ttcagccggg gatccaagac tcgccaaagt
     2821 aacggcgact gcatttttca cgcgatcgtc ggtgtcgtgc aaataagtcg tcagaagctt
     2881 ttgccggacc gtggcatcta cgtcggtcct taccttcata cttgtaatgg cggcgacgcg
     2941 tactgtcgct tcatcgtcgt ccaggcgctt cagcattgct gccctggtct cggcgtcgtt
     3001 cagcgctaag tcacgtaagc agtaaagcgc catgcgtttc tggttcgagc tgccggtttc
     3061 gcagagtgag accagaaggg gaactacggc aggctcccgc tgcgcgatgc gcacgagcag
     3121 caggccattg gcccagcgaa tatccggttc gcgatgatcg agggcgctga gcagtgtccg
     3181 aacgacaacg ccagagggct gactttgatg gccgagaaca taagcgacgg gccaatagtt
     3241 tttatgatgc gcggcctgga gccgttcgtt tagcgcgtca gtgagttccg ctgaactccc
     3301 cgctaacccg atgagtttgt cgacggcggc acgaatcgtc ggtttatcgg gatgatcgag
     3361 ggcagcgata agatcggcga tctcgcccgg atcggttgtc atgtgcgaac gccagagcta
     3421 gacctggctc aattaaattt ccaggaactg cgggcagtca gtcaagttgc ggttgccttt
     3481 gggcttaacg acgacgacgt cctccagtcg gacgccgccg actccgatgt aatagaggcc
     3541 gggttccacc gtgaccacat gacccgtgcg caggacagcg tcgacaggag ctatgcgcgg
     3601 cggctcgtga atgtccaaac ccaaaccatg cccggtgccg tgaaagaatc cctgcatgcg
     3661 gccattaatc ctgccggtgg gaaaaccgcg ggcggcgaac atgttcaaaa tattttggtg
     3721 cacttccttg ccgtttacgc cgtcgcggat ttgctcgtag ccgatctgct ggccggcttg
     3781 cacggtggcg taaatttcct ttagcttttc cgacgcgcgg ccgcgcacga cggtccggct
     3841 taggtcgccg aaataaccat tgtgttgcga gcgcggaaag atatcgaaga tgatcgaggt
     3901 attagcccgg atcgggccag tgccttcatg gtgcggatcg acgcattgat tgcccgagga
     3961 aatgatcgtg tgactcggta gccacccctg cgccatgatg gtcgtgttga ccgcggtctt
     4021 gagcatctcc gaagtcagcc gcgtaccgtt gagatataga taaccatcgc gtttaggttt
     4081 tgtccgccgg agcatgcgga tgcccgattc caacccgagc cgggcgatgc gcagcgattc
     4141 ggtgatctgt ttcacttcaa gcgtgttctt ggtctcgcgt tgtggccaga aagggtcgcg
     4201 cttgatctgc acgctgtaac ccttagcgcg caactggtcc aacaatagcg cggagaaatt
     4261 cgccggcacg ataagcgagc gaatgccgcg ctcgcgaaaa atcagatcaa ctatgtcaat
     4321 gattcctgcc ggggtcttgc cgagcttacg caattttttc tgatacaggg ataaagacac
     4381 gactcggtcg gcgtgcgctt gtttgttggc gcggtcgatc tccaggtcgc tcatgacgat
     4441 gtggcggcga tttttatgct caaagaatat aaacggatcg ggcacgaaga aaccggtggc
     4501 gtaaagcatg ttggcgtcgc cttcactggc ggcgtaaagt aggatcgctt gattcggttt
     4561 taggtggatc ggttgcggtg ccgatccgct cgatttgttt tgcggttttt ccttcgcttc
     4621 gaacatagtc gtcgtcacgc ctcgattcgt attggttaac ttaggccttc aggcttggcg
     4681 tagccgcgac ggcgccgcca atcatcttaa tcgaaagaat accacaagct cgagtggctg
     4741 tgtatctacg gtaaatgctc cttaagcaat ccgccgttgc cagataattt tctcgggagt
     4801 tcggtcacgc taacaagttg attctcgaac cgcaagtttg cttgtgaatt cgcgcgattc
     4861 agttgacagt cgaatgcctt ttgttatgat gccgccactc gctggcgaaa ctattttgat
     4921 tcgcgtctaa ggaatctctc tccatgacga tagccaacaa tctcgacaag ctgcgcgaac
     4981 tcaacgacaa ggctgaagct ggcggcggcg cggagcgggc gcgacggcag catgaacagg
     5041 gcaaactttt ggcgcgcgag cgtctcaaca ttctgctcga tagcggcagc ttcgtcgaga
     5101 tggatcgctt gcgcacgcac cacagcactg actttggcct cgagaaacaa aaatttttag
     5161 gcgacgcggt ggtgaccggc tccggtgcca tcgatggccg gctcgttttt gtttactccc
     5221 aggacttcac cgtcttcggc ggcagcttga gccgcgtggt tgccgacaag atctgcaaga
     5281 tcatggatat ggcgatcaag aacggtgcgc cgctcatcgg catcaacgat tccggcggcg
     5341 cccgtattca agaagggatc gatagtctcg ccggctatgg tgatatcttt caacgcaatg
     5401 tcatggcttc aggagtagtg ccgcagatca cggcgatcat gggaccgtgc gccggcggcg
     5461 cagtctattc gccggcaatc acggacttca tcttcatggt gaagaccaat agctacatgt
     5521 tcatcaccgg tccggatgtg atcaagaccg tgacccatga agaggtatcg aaagaggatt
     5581 taggcggagc ggagacgcat acggccaaga gcggtgtggc ccattttgcc gccaacacgg
     5641 aaaaagactg cttgctgatg atccgcgagc tggtgagttt tttgcccagc aacaatcagg
     5701 aagatccgcc gttccgtccg accaacgacg atccgctgcg gcgcgacaag cggctcaaag
     5761 acattattcc agacaatccc aaccggccct acgatatgaa agaattaatc gttggggtcg
     5821 tcgacgaagg gtatttctac gaggttcaac cggagttcgc gagaaatata ctcatcggct
     5881 ttgctcatat cggcggtcgc tccatcggca tcgtcgccaa tcagccgaat aatttggctg
     5941 gctgtttgga catcgacgca tcggtgaagg ccgcgcgctt cattcgtttt tgtgattgct
     6001 tcaatattcc catcgtcact tttgtcgacg tacccggctt cttgcccggt accgagcagg
     6061 aatatcacgg catcatccgc catggcgcca aattgcttta cgcctacgcc gaagcgacgg
     6121 tacccaaggt gactatcgtg acgcgcaaag cctatggcgg cggctatatc gtcatggcga
     6181 gcaagcattt gcgcggcgac atcaatttag tttatcccac cggcgagatt gccgtgatgg
     6241 gaccggaagg g
//
LOCUS       DATLCH010000358         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7418282, whole genome shotgun sequence.
ACCESSION   DATLCH010000358 DATLCH010000000
VERSION     DATLCH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7418282"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VMZ02_10605"
     CDS             complement(<1..455)
                     /locus_tag="VMZ02_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HUR72429.1"
                     /translation="MKEVRGVVLPSPTVFREDGSIDEPLMRELTDWFVACGVQAFFIL
                     GSYGQGAAMQTEERKRVAEIIVQQVKHRVPVVVHVGAVDPYTAIDLGRHAKAIGAEAI
                     GHVGPYYYADRSEYEIIEHLKMVDRAVGMPMLIYNNPRYSGYNIHPHFMA"
     gene            complement(559..1338)
                     /locus_tag="VMZ02_10610"
     CDS             complement(559..1338)
                     /locus_tag="VMZ02_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR72430.1"
                     /translation="MTIQLERGKKYPPQEAKAIVETLYGKVRERWEARVNQKPFMQQL
                     MTGKLPRKTLQLFFRNWGAYTIEINTLTACTYHRNITFLKVNRDLMGPIGEKIADEFI
                     HPKPPGHFLVMMETAKALGLSEDEVFTSSMLSEFRGKIDFMRSIVYEGSAAEWYAAVT
                     TEEQIGHWSAACYKALTSHYGFDRAKATYFATHVEADLEEHEAGVMGHASFNRMALQR
                     LLETGMAEERPTYGLEYCAMTSVDLHGAMLRAAMEEAERGN"
     gene            complement(1461..2408)
                     /locus_tag="VMZ02_10615"
     CDS             complement(1461..2408)
                     /locus_tag="VMZ02_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72431.1"
                     /translation="MEYRIKRIHFLAGLVGILCAFTTSWAAQPTKVRMSYSSRSNSNT
                     PFVIALRKGFFADEGMDVEMIQVNPRLGATALLNGDIDFTATFGSTLRSIVGGFPIKF
                     VAVSVKKSEHFLIVRPEIKEIRDLNGKKLGVATLFGSDQKAAEEMIRSKGFSPSMVKP
                     ISLGEAPVRAQALRSGVVEAISVSSPFDLSLQNEGFRALAGPKDVEFALPTSGIAVTN
                     KLLQQNPQHVKRVVRALMKAHRFVFDNKKEVVPEMMRYLDQTNQVAERSYDLIVNSLS
                     RNGEITDQEWELLTEKKKTAEEVRDFALLREVQKELKIR"
     gene            complement(2424..3662)
                     /locus_tag="VMZ02_10620"
     CDS             complement(2424..3662)
                     /locus_tag="VMZ02_10620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HUR72432.1"
                     /translation="MFQTQNSRIASTLNKPLNLSLALWIPRRVRIENRGIHFHMQHFA
                     YVIVGAGFAGAATAYHLARQGVEDVLVLEQESIPGFHASGRNAAMLRQCIGEADLTKL
                     ALDGATFIRNLPDDWPEPVLFRNNGSLLLGSGAGWDKLKRDGEIGRRVGISGIAEWSP
                     EEAIRHVPLLKEAEFDGALWCASDGVVDIHALLSGYLKFAVSRGTKIRYNAALSGARV
                     KPGADIELQTADGLVSCKVLVNASGAWANGVAHLAGARALPLRACRRHLFVSPALPWV
                     DREWPFVWDVSHDIYFRPEGAGLLLCACDQTELLASDPPLDESVKEMLAEKIQLYMPG
                     LSEVSISRGWAGLRTLTADGRFVVGWDADIAGFFWVAGLGGHGMTTSAAIGKLAAELL
                     IGGPGRKSVPFAPERFGAKT"
     gene            3691..>4859
                     /locus_tag="VMZ02_10625"
     CDS             3691..>4859
                     /locus_tag="VMZ02_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72433.1"
                     /translation="MRIHRYTQWDGTQEILFPTTDDIINQLSDHLLEEEGVRRALRDM
                     MRRGFTSEDGQRSVKGLRDFLRQADEKRRELLNKYSPDSFKLTAEEQQKLSEKLSSLA
                     EKMEAYNEQMRNFMERMSGKFSDRMDELNQKMQEAYQRYQELQQRLKQQIQERGMQGQ
                     SDMSGQQNPQSLMDMLDRMSQLLNDENFLKNLPSMMDNAINNLESMLENMGNMTEEQL
                     QQMSDMMNQMQQLEQLMNQFPFQGSQRMGMGEAGQVLSQLRGLERFLRWGQRGMGDPS
                     DLNLDELRELLGEEAYENLKYLQNVEQMLEEEGYIVRGGHGLKLTAKGMRKIGDKALR
                     EIFQMLNKSRWGNHDTSARGSQGEHMEETKLYEYGDPMNINISETVLNALERRKQG"
BASE COUNT         1118 a         1435 c         1284 g         1022 t
ORIGIN      
        1 gccatgaaat gcggatggat gttatagccg gaatagcgcg ggttattata gatgagcatt
       61 ggcatgccga ccgcgcgatc gaccattttc aaatgctcga taatttcata ctcggagcgg
      121 tcggcgtaat aatacggtcc gacgtggcca atcgcctcgg cgccaatcgc cttggcatgc
      181 cgccctaaat cgatcgccgt atagggatca accgcgccaa catgcacgac caccggaacg
      241 cgatgcttca cttgttgaac gatgatttcg gcgactcttt tacgctcctc cgtctgcatc
      301 gccgctccct ggccgtagga acccagaata aaaaaagcct gcacgccgca ggcgacaaac
      361 cagtcggtca gctcacgcat aagcggctca tcgatgctgc catcctcgcg aaacacggtc
      421 gggctcggca aaaccacgcc tctaacttct ttcataaaat cctccggaat cttttttgcg
      481 ccttttgcgc tctttgcggc caatctcagg caggaatctt ttttctgtgt gttctttgtg
      541 ctctttgcgg ttaacttctt aattcccccg ttccgcctct tccatcgccg cacgcagcat
      601 ggcgccgtgc aaatcaaccg aggtcatcgc gcagtattcc aggccgtagg ttggccgctc
      661 ctccgccatg ccggtctcaa gcaagcgctg caacgccatg cgattgaaac tggcgtggcc
      721 catgacgcct gcttcgtgtt cttccaaatc ggcttcgacg tgggtggcga agtaagttgc
      781 cttcgcgcga tcgaagccgt agtggctggt caaggctttg taacaggccg ccgaccaatg
      841 gccgatctgt tcctcggtgg taactgcggc ataccattcc gcggcggaac cctcgtaaac
      901 gatgctgcgc atgaaatcaa tcttgccgcg gaactccgac agcatggatg aggtaaacac
      961 ttcgtcctcg ctcagaccta aagctttcgc tgtctccatc atgacgagaa aatggcccgg
     1021 cggcttggga tgaatgaact cgtcggcaat cttctcgcct ataggtccca tcaggtcgcg
     1081 gttcactttt agaaaggtga tattgcgatg ataagtgcag gcggtcaacg tattgatctc
     1141 gatggtgtac gcgccccaat tgcgaaagaa cagctgcaag gttttccgcg gcagcttgcc
     1201 ggtcatcaat tgctgcatga acggcttttg attgacccgc gcttcccaac gttcgcggac
     1261 tttgccatac agtgtttcga caattgcttt ggcctcctgc ggcggatact tcttgcctcg
     1321 ttctagctga attgtcatgc ggcacctccc ggttggtgga cgtccttgaa agtaaaaatt
     1381 tcaaattatc gggcagcccg gatcgtctgt cgacttgcgg ggatcggcca tcggatcgaa
     1441 agcacattcg ttggtttgag tcagcgaatc ttcaattcct tctgcacttc gcgcagcagc
     1501 gcgaagtcgc gcacttcctc ggccgtcttc tttttctcgg tcagcagttc ccactcttga
     1561 tcggtgattt ccccgttgcg gctgagcgag ttgacgatga gatcatagga tctctcggcc
     1621 acctggttgg tctggtcaag ataacgcatc atctccggta ccacttcttt tttgttgtca
     1681 aagacaaaac ggtgcgcttt cattaaggcg cggaccacgc gcttcacatg ttgcgggttt
     1741 tgctggagca gcttgttggt aacggcgata cccgaagtcg gtagagcgaa ctcgacatcc
     1801 tttggtcccg cgagtgcgcg aaagccctcg ttttgcaggc tcaaatcgaa cggcgaagaa
     1861 accgagatcg cctcgaccac ccccgagcgc agcgcttgcg cccgcaccgg cgcctcgccg
     1921 agtgagatcg gcttgaccat gctgggggaa aaacccttac tgcggatcat ctcttcggcg
     1981 gctttctgat ccgaaccgaa aagtgtcgcg acgcctagtt tcttcccgtt caaatcgcgg
     2041 atttctttga tctccggcct gacgatcaaa aagtgttcag actttttcac cgaaaccgcg
     2101 acaaacttga ttggaaagcc gccgacgatg ctacgcaagg tgctaccgaa tgtcgcagta
     2161 aaatcgatat cgccattgag cagcgcggtc gcgcccaggc gcggattgac ttggatcatc
     2221 tccacatcca tcccctcatc ggcaaaaaat cctttgcgca gggcgatgac gaacggcgta
     2281 ttcgaattgc ttcggctcga gtacgacatg cgcaccttgg tcggctgcgc cgcccagctc
     2341 gtcgtgaaag cgcacaggat accgaccagc cccgccaaaa aatgaattct ttttatccga
     2401 tactccatta ctgcaacact ctcttatgtt tttgcaccga atcgttccgg cgcgaacggc
     2461 accgacttgc ggcccggccc gccaatcaat agttccgcag ccaatttacc gatcgccgcg
     2521 cttgtggtca tgccatggcc gccgagaccg gcgacccaaa aaaagcccgc gatgtcggcg
     2581 tcccaaccaa ccacgaaacg cccgtccgcc gtcaaggtac gcaatccggc ccagccccga
     2641 ctgatggaaa cttcggacaa cccgggcatg tagagctgga tcttttcggc gagcatttct
     2701 ttgacgctct cgtcgagcgg tggatcgcta gccaacaact cagtctgatc gcaggcacag
     2761 agcaacaatc ccgcgccttc cggacgaaaa taaatatcat ggctgacgtc ccaaacaaaa
     2821 ggccactcac gatcgaccca gggcagcgcg ggcgaaacga acaggtgacg ccggcacgcg
     2881 cgcaacggca gcgctcgggc gccggctaaa tgcgcgacgc cgttagccca tgcgccgcta
     2941 gcattcacca agaccttgca gctgacgagt ccatcggcag tttgcaattc gatatccgca
     3001 ccaggcttga ctcgtgcccc ggagagcgcc gcattgtagc gaatcttcgt cccgcggcta
     3061 acggcgaatt ttaagtaacc ggacagcagc gcatgaatgt cgacgacacc gtcgctggca
     3121 caccacaagg ccccatcgaa ctcagcttct ttgagcaacg gcacatggcg gatcgcttct
     3181 tcgggcgacc actccgctat gccgctaatc ccgaccctcc ggccaatctc gccgtcgcgt
     3241 ttgagcttgt cccagcccgc gccgctgccg agcaaaagcg aaccgttatt tctgaataat
     3301 accggctccg gccaatcgtc aggtagattg cggatgaatg tcgcaccatc tagcgccagc
     3361 ttggtaagat cggcctcgcc gatgcactgg cgcaacatcg ctgcattgcg gcccgaggca
     3421 tgaaaccccg gaatactttc ttgttccaaa actaagacgt cctcgacgcc ttgacgggcc
     3481 aggtgatacg cggttgccgc tccggcgaac ccggcaccga cgatgacata ggcgaaatgc
     3541 tgcatatgaa aatgaatccc tctattttct atacgaactc ggcgcggaat ccaaagggca
     3601 agcgagagat tcaacggctt gttcaacgtc gacgcgatgc gcgaattttg ggtttgaaac
     3661 atcccggaac tgcgctatag ttcaagccaa atgagaatcc accgctacac ccagtgggat
     3721 ggcacgcagg aaattctctt tcccacgacc gacgacatca tcaatcaact ctccgatcat
     3781 ctcttagagg aagaaggcgt gcgccgcgct ttgcgcgaca tgatgcgccg cggctttact
     3841 tccgaagacg gccaacgctc ggtcaaaggt ctgcgcgact ttctgcgcca ggccgacgag
     3901 aagcgccgcg aactgctcaa taaatactcc cccgactcgt tcaaactcac cgccgaagag
     3961 cagcaaaagc tcagtgaaaa gctcagcagc ctcgccgaga agatggaagc ttacaacgaa
     4021 cagatgcgca acttcatgga gcgcatgtcg ggcaagttct ccgatcgcat ggacgagttg
     4081 aaccagaaga tgcaggaggc ctaccagcgc tatcaggagt tgcagcaacg tttgaagcag
     4141 cagattcaag aacgcggcat gcagggccag tccgatatga gcggccagca aaacccgcag
     4201 tcgctgatgg acatgctcga ccgtatgagc cagctgctga acgacgagaa ttttctcaag
     4261 aatttgccga gcatgatgga caacgccatc aacaacctcg aaagcatgtt ggaaaacatg
     4321 ggcaacatga ccgaggaaca gctgcagcag atgagcgata tgatgaatca gatgcaacag
     4381 ctggagcagt tgatgaatca gttcccgttc cagggctcgc aacgcatggg catgggcgag
     4441 gccggccagg tgttgagtca attgcgcggc ttggaacgat ttctccgctg gggccagcgc
     4501 ggcatgggtg atccgtctga tctgaatctc gatgagctgc gcgaattgct cggcgaagaa
     4561 gcctacgaga atctcaaata cttgcagaac gtcgagcaga tgctcgagga ggaaggctac
     4621 atcgttcgcg gcggccacgg cctaaagctg accgccaagg gcatgcgcaa gatcggcgat
     4681 aaggctcttc gtgaaatctt tcagatgctc aacaaaagcc gctggggcaa tcatgacact
     4741 tccgcgcgcg gctcccaggg cgagcacatg gaagaaacca aactctacga gtatggcgac
     4801 ccgatgaaca tcaatatcag cgagaccgtg ctcaacgcgt tagagcgacg caagcaagg
//
LOCUS       DATLCH010000359         3498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7988178, whole genome shotgun sequence.
ACCESSION   DATLCH010000359 DATLCH010000000
VERSION     DATLCH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7988178"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VMZ02_10630"
     CDS             complement(<1..930)
                     /locus_tag="VMZ02_10630"
                     /inference="COORDINATES: protein motif:HMM:NF040245.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HUR72434.1"
                     /translation="MLTSETKEPYLAAFKRMVEAGTFSGPAWVGELRQAGIASFNGLG
                     FPTLKNEDWKYTNIEPIAAQSYVQPNGEVQSASGTELLSRALIETDAPRLVFVNGVYV
                     SALSSTDGLQQGLRLGSLADFIKQNNPAVAQELGRHASAERQHFVALNTAFLSEGAVV
                     EVAPGCRLERPIYLVLASTSTNQPVISHPRILLLLGAGSEAKIVESYVGVRGKEYFCN
                     AVTELVGGADSVVEHFRLQQESDKGFHVGTVAASLARGCHLTAHAVTLSGALVRNNVR
                     VLLNGEGAECVLNGLYLGDGKQHIDNYTLIEHVS"
     gene            complement(955..1713)
                     /gene="sufC"
                     /locus_tag="VMZ02_10635"
     CDS             complement(955..1713)
                     /gene="sufC"
                     /locus_tag="VMZ02_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HUR72435.1"
                     /translation="MLDIKNLHANVGDKEILRGIDLHIAPGEVHAIMGPNGSGKSTLA
                     HVLSGRENYQVTAGSVTYQGKDLLTMAPEVRAREGVFLAFQYPIEIPGVSTTYFLKAA
                     LNAVRKHRGLDELDAMEFLALIKDKMTLVQMSQALLNRPLNEGFSGGEKKRNEILQMA
                     VLDPTLAVLDETDSGLDIDALRVIAGGVNALRSPSRAMIVITHYQRLLDYIVPDFIHV
                     LYDGRIVKSGTKELARELESKGYDWIKSEVAAVP"
     gene            complement(1729..2181)
                     /locus_tag="VMZ02_10640"
     CDS             complement(1729..2181)
                     /locus_tag="VMZ02_10640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HUR72436.1"
                     /translation="MNVGRRVDYAVRALSYLAGQPDGTIVSRSDIEKNQDVPSFYLSK
                     IMKDLVAGGLVESHIGSKGGFTLAKKATSITIKDIYETVERPLVLMECLEQGASYCSF
                     CTVCKQKSIWEEAQSVLANFLAGVTIADIADRQGMKGRMLKTPMQRVG"
     gene            2353..2886
                     /gene="def"
                     /locus_tag="VMZ02_10645"
     CDS             2353..2886
                     /gene="def"
                     /locus_tag="VMZ02_10645"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HUR72437.1"
                     /translation="MAILKVTRLGHPVLRKITENISLKELKSAAMQRFIDDMVETMKE
                     YDGVGLAADQVFTSKQVAVLEVADNPRYPNKDKVPMTVLVNPKITPLSEDMEDDWEGC
                     LSIPDLRGAVPRYKRIHVTALDRDGNKLDFIAKDFHARVIQHEYDHLNGRVYLDRMRD
                     FSTLTFLQEFARYWINK"
     gene            complement(2944..>3498)
                     /locus_tag="VMZ02_10650"
     CDS             complement(2944..>3498)
                     /locus_tag="VMZ02_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HUR72438.1"
                     /translation="HLLNKKNTFTDFIACAEYLIDQSFTQPDKLFAMGRSAGGLLMGA
                     VSNMRPDLFRGIVAEVPFVDVVTTMLDASIPLTTGEYDEWGNPDSKEFYDYMLSYSPY
                     DNVAPKNYPAMLITGGLHDSQVQYWEPAKWAAKLRELKIGTNPVLLKTNMDAGHGGAS
                     GRFRRHQETAFSYAFLLDLAGIGE"
BASE COUNT          805 a         1038 c          885 g          770 t
ORIGIN      
        1 actgacgtgt tcgatcagtg tgtagttatc gatatgttgc ttgccatcgc ccaggtaaag
       61 cccattgagc acgcactcgg cgccctcgcc gttcaacagt acccgcacgt tgttgcgcac
      121 cagtgcgccg ctcaaggtca ccgcatgagc agtcaaatgg cagccacgcg ccaggcttgc
      181 cgcgaccgtg ccgacgtgaa aacccttgtc gctctcctgt tgcaagcgaa aatgctcaac
      241 gaccgagtct gcgccgccga ccagttcggt gacagcatta cagaaatact ccttgccgcg
      301 cacgcccaca tagctctcga cgatcttcgc ctcactgccg gcgccaagca aaagcaaaat
      361 gcgcgggtgc gaaatcaccg gttgattcgt cgacgtagaa gccaacacca agtagatcgg
      421 ccgctcaagc cggcaacccg gcgcaacctc caccaccgcg ccctcgctta aaaacgcagt
      481 attgagcgca acaaagtgct gccgctcagc gctcgcatgt cggcccaatt cttgagcgac
      541 cgccggattg ttctgtttaa tgaaatcggc cagcgagccc aagcgcagcc cctgctgcag
      601 tccgtccgta cttgaaagcg ccgagacata aacaccgttg acgaacacca agcgcggcgc
      661 gtcggtttca atcagcgcgc gggatagcag ctcggtgccg ctagccgact gaacttcacc
      721 gttgggctgg acgtaagatt gcgccgcgat cggctcgata ttggtatatt tccagtcttc
      781 gttcttgagc gtcggaaagc ccagcccatt aaagctggcg attcccgcct ggcgtagctc
      841 accgacccac gcgggtccag aaaacgtacc ggcctcgacc attcgcttga atgcggctag
      901 atacggttct ttagtttcgc tcgtgagcat ggatttgtta tatactcgta gaaattatgg
      961 caccgcggca acttcgctct tgatccagtc gtagccttta ctctcgagtt cccgcgccag
     1021 ttccttggtt cctgacttga cgatccggcc atcgtaaagc acatgaataa agtccggcac
     1081 gatataatcg agcagccgct gataatgtgt aatcacgatc atcgcacggc tgggactgcg
     1141 cagcgcattg acgccacccg cgatcacccg cagcgcgtcg atatctagtc ctgaatcggt
     1201 ttcatcgagc actgcgagcg tcggatcgag caccgccatt tggagaattt cgttgcgctt
     1261 cttctcgccg cccgaaaagc cttcgttaag cggccgattc aacaacgctt ggctcatctg
     1321 caccaaagtc attttgtctt tgatcaaggc taagaactcc atggcgtcga gctcgtcgag
     1381 accgcgatgc ttgcgcacgg cattgagcgc tgcttttaga aagtaagtgg tgctgacgcc
     1441 gggaatctcg atgggatatt gaaacgccaa aaaaacccct tcacgggcgc ggacctccgg
     1501 cgccatggtc agcagatcct taccctgata agtgaccgaa ccggcggtga cctggtaatt
     1561 ctcacgcccc gataaaacat gggccaacgt gcttttgccc gaaccattag gtcccatgat
     1621 cgcgtgcacc tcgcctgggg cgatatgcag gtcaatgccg cgcagaattt ccttgtcacc
     1681 gacattggcg tggagatttt taatatctaa catccggatt caccccagct atccgactct
     1741 ttgcatcggc gttttgagca tccgcccctt catgccctga cgatcggcaa tgtcggcaat
     1801 cgtgacaccc gcgagaaaat tggcaagcac cgattgcgcc tcttcccaaa tcgatttctg
     1861 cttgcagacc gtgcagaaag aacaataact cgccccctgt tcgaggcact ccatcaatac
     1921 caacggccgc tccacggttt cgtaaatgtc tttaatggtt attgacgtgg ccttttttgc
     1981 cagcgtgaac ccgcccttgg agccgatgtg cgactccacc aaaccaccgg caaccagatc
     2041 tttcatgatt ttggacaaat aaaatgatgg tacgtcctgg ttcttctcga tatcggaccg
     2101 gctgacgatg gtcccgtccg gttgcccggc taggtaagac aacgcgcgta ccgcgtaatc
     2161 gacacggcgc ccgacgttca tcaacgaact tcgttcgctt ttcgcctttc gcttcacgtt
     2221 tttaaatata gactcgttag gtctactatg tcaagataac cgccgtctgc ggacccgatt
     2281 gttatgcgca gcactcgtcc gatcgcacgc cgtggctttt tcccaccgtg tgtccatgct
     2341 aaaaagctgg ccatggcgat cctcaaagtc acgcgcttag ggcatccggt gctgcgtaaa
     2401 atcactgaaa acatttcgct caaagaactg aagagtgcag caatgcaaag attcatcgac
     2461 gacatggtcg agaccatgaa agaatacgac ggagtcggct tggccgcgga tcaggtattc
     2521 acatccaaac aggtcgccgt tctcgaggtc gccgacaacc cgcgatatcc caacaaagat
     2581 aaagttccga tgaccgtttt ggtaaatccg aaaatcactc cgctgtccga agacatggaa
     2641 gacgactggg agggctgcct gagcatcccc gatttgcgcg gtgcggtgcc gcgctataaa
     2701 cgaatccacg tcaccgcgct cgatcgcgac ggcaataaac tcgattttat cgccaaggac
     2761 ttccacgccc gggtgatcca gcatgaatac gatcacctga acggcagagt ctacctcgat
     2821 cgcatgcgcg atttttcaac gctgacgttt ctccaagaat tcgcccgcta ttggatcaac
     2881 aaatgacgac gcgacgcgtt gtcgcgaggg actactagca ggtagatcaa gaacttaaag
     2941 ccgttactca cctatccctg ccagatcgag caaaaaggca taactaaacg ccgtctcttg
     3001 atgacgccgg aagcgtcctg aggcgccgcc gtggccggca tccatgttgg ttttgagcag
     3061 aacaggattg gtgccgatct tgagctcgcg cagtttcgcc gcccacttcg ccggctccca
     3121 gtactgcacc tgagaatcgt gcaggccgcc ggtgatcaac atggccggat agttcttagg
     3181 cgcgacatta tcatagggcg aataggacaa catataatcg taaaattctt tgctatcggg
     3241 attgccccac tcatcatact cgccggtggt cagcgggatc gacgcatcga gcatcgtcgt
     3301 gacgacgtca acgaatggca cctcggcgac aatgcctctg aacaaatccg gccgcatatt
     3361 gctcaccgct cccatcaaca agccgccggc gcttctgccc atggcaaaga gcttgtcggg
     3421 ttgggtgaac gattgatcga tcaaatactc ggcgcaagcg atgaaatcgg tgaaggtgtt
     3481 tttcttgttg agcagatg
//
LOCUS       DATLCH010000360        11474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6499892, whole genome shotgun sequence.
ACCESSION   DATLCH010000360 DATLCH010000000
VERSION     DATLCH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6499892"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VMZ02_10655"
     CDS             complement(<1..224)
                     /locus_tag="VMZ02_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72439.1"
                     /translation="MKKLLIVFHSMTGGTYQMAQAAASGAQSEPEIAVNLIHAAEAGA
                     DEVLQAGGYIFATPENLAMMSGVMKDFFDRT"
     gene            complement(357..1277)
                     /locus_tag="VMZ02_10660"
     CDS             complement(357..1277)
                     /locus_tag="VMZ02_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72440.1"
                     /translation="MRTVRLAYRDHDRTPVIYCIKAMAERHYRIDVEVLHIEPQREFE
                     AALFDHACDVIIEHIEYLHTEAAKGKKATLFCAPQIQRGLKLVVPQSVNSLDDLKGKT
                     MAVRDLGRPHRITLWLRKVGLENSVKTIIVADKEVGRWQQWRKIVSGECVACFMAPIY
                     LPAALAAGLKTFPASEAEIVSLFAQACLSEFGRNNSTLIKDYLKAVIHALALLIYRRE
                     EAMEIIVQEPMRLMKITDLSELRRQVDSIAELLQIRPYPTIEAILNTDEIAAQEYGPT
                     IDNPLTLWDLHWLKELDDEGFIDNLAASLR"
     gene            complement(1404..2120)
                     /locus_tag="VMZ02_10665"
     CDS             complement(1404..2120)
                     /locus_tag="VMZ02_10665"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72441.1"
                     /translation="MKIGFLGSSAPSSPHHASFKKFIPADIDFTFLQESGTHTSLYDA
                     QGKVDALIEQSRELAESNGWNGLIISGAPKEALNPGMWEQVSTALNVPVALALRSSVA
                     ALKAYSARKILLMTPVDDRLKKMYHDYLAEFGVESFYPPQILRAHTDAQKLTSSDVEA
                     MTRKALSEYPNVDAIYFQGALLDPIPLIDKLESELKLPIVASNPAMLWLVLSKLGRRY
                     SIAGYGQLLETWPAVPAGPF"
     gene            complement(2199..2384)
                     /locus_tag="VMZ02_10670"
     CDS             complement(2199..2384)
                     /locus_tag="VMZ02_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HUR72442.1"
                     /translation="MPVVHIYMFPGKSKEQKNELVKRISKDFAEVANIKPESLNILFH
                     DTGKEDWGVRGSLASEQ"
     gene            2522..2710
                     /locus_tag="VMZ02_10675"
     CDS             2522..2710
                     /locus_tag="VMZ02_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72443.1"
                     /translation="MERTLVTWLGFSFAERQLFLIKSGIFLSVCGATLFVALASSYPA
                     VHDTLHNVRHALAVVPCH"
     gene            2734..3687
                     /locus_tag="VMZ02_10680"
     CDS             2734..3687
                     /locus_tag="VMZ02_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72444.1"
                     /translation="MSCSLLVGVAASAEELKIYLKTTPALEFLRPFADPVTMSLLVTR
                     ADGRPLERGSVGIVLDAPKSGAFLSTDFPVVENSRLLELALPLRQGRAGWKYLFPIRG
                     DYRLSVNVVGSDGTKTAKNFVIPVLEDRTKWLSLGLFCAALFILGFTAGRIFTAAPAT
                     MTTAWLIFSCLVTAVSGHDGPVSVENFAPPPTALEIAAATVGKPTQLRWRAPDGGAAS
                     TLLSISITHLEKHKMVFAVDNVPVDKDYLLDFQFPDGAEYRVNSIAQFPGRGPLRTEQ
                     LVAVTGVEPPMAAQVPALALFLAIIALGLGAGRFSKILAAR"
     gene            complement(3761..4537)
                     /locus_tag="VMZ02_10685"
     CDS             complement(3761..4537)
                     /locus_tag="VMZ02_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR72445.1"
                     /translation="MAIVTSNASHEGDFYHDIFGMKRSNPTRAGGAVVVADGYVGLNL
                     NPRAPGRQAGFDHFGFEVQDVEVVFGRLKEKYPKIQVLHRPSNRPFAGISMHDPNGNV
                     FDLSQLNMENRRDLYTEKIEERSRRVGHIGLRCVDPDLCGEFYRDVFELTELEKPNGD
                     ANHYLSDGKVTLAVMPWKISDFAGSGIERPALDHIGFKVESLAAFKADLAKHAHLYAA
                     EEIRPEKNPEREVRQNLLATCCFGQYQMSDPDGVLIDVSE"
     gene            complement(4712..6175)
                     /locus_tag="VMZ02_10690"
     CDS             complement(4712..6175)
                     /locus_tag="VMZ02_10690"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HUR72446.1"
                     /translation="MAKMFIGGESVDSVTKNTSEVRNPATGATVDTVPQGNDKDVQQA
                     IAAAEDAFKVWADVSPEDRGSALMNAGELIKKNTSEIAQLLTQEQGKTLFEAGLEIHH
                     LVHGLEFYAGLASKVRGSHVPLPQKNAYGMVVRQPIGVCGGIVPWNFPLTLMGTKLGP
                     ALAAGNTIIIKPASTTPLATIKVIELIAQATFGEGKAQKQLPKGTVNIVTGPGGSVGE
                     ELLRNPRVRRIAFTGSTPVGRHVMEVAGREIKRVTLELGGSDPMIVMEDANVDLAVKM
                     ADVGRYFNCGQMCLGVKRLFLQESIADNFIGGLVEILKKKTVGNGTSKETRMGPIHIG
                     SQRQEVEEQVEDAKSRGGKVLFGGERPKGAEFDKGFFYLPTLMTNVPEDSRLTKEEVF
                     GPALPVYTFKTLDEALEKANASEFGLGSSIWTRNLLHANKAIDKLQAGNVWVNSLHYG
                     YDELPFGGVKSSGVGREHGPEALDYYLEPKGVVVVNV"
     gene            complement(6314..7096)
                     /locus_tag="VMZ02_10695"
     CDS             complement(6314..7096)
                     /locus_tag="VMZ02_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUR72447.1"
                     /translation="MAMIESRIDSGIAVLEINRPPVNSYTTELLKELDAAVLDARFND
                     DVHAIVITGKVEKFFSAGADINMLSSKSLGFKNNFALHGHEVLMRLENTPKIIIAAIN
                     GHAVGGGLEIAMACDIRIAKKEGGRVGLAEINLGVMPGMGGTQRLPRLVGKARAMELC
                     FTGKTIAFEEALEMGLVHYIYDKEGFMDQVMDYARQFIVPNKSSLAVGKVKRAITTGL
                     ELSLPDGLAFERELLAQTFGSDDGQEGVKAYLEKRTAKFTGK"
     gene            7333..8511
                     /locus_tag="VMZ02_10700"
     CDS             7333..8511
                     /locus_tag="VMZ02_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72448.1"
                     /translation="MARKDSHSEQLKKGLGALAAAGIDVDPPSVAAIAQLRALWRQSP
                     EMDLATIFLLGKLASVEAAELLRELDRDIGDKELKKEVKRSLFKLSQKGFAVPQEQAA
                     AKQPTPVFERENQTEAYMSAVDGGGGRLIWLAKPQPQRGLQVIQAMVHDSEGLVRFGG
                     GQMKRRELRAMADDIKQQHGVIMIAIPWEFADWIVYEGYEMAKARGQSGLEDFHAIRS
                     VIGAGKPKPVTHPIYELLGAGDFREGPWREQSRRLLEEPELRYWVITDEWVQDCLTAL
                     HEAQSSPLILNQVQKEERLAAVVRDAVKTLCAGDRGRAFKRRMEDMALYFAKTERSEK
                     ARLALAVAQQVAEGDPGPLDISFLTGLMQKSFVFFMSQLKAQHEAQEKERKSSLIIKP
                     "
     gene            8654..10120
                     /locus_tag="VMZ02_10705"
     CDS             8654..10120
                     /locus_tag="VMZ02_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HUR72449.1"
                     /translation="MIYREIKRRAKQDPDKIIIIGERRNLDYRQLLKEIDACAAYFQS
                     VGIKAQSSVLIGVPPAPEFYVAFFAACAVGATAIPLLPSAAGIAAPVRDSKPAMAIGS
                     RAFINAVRSQLPSICDELLWDAKNGLHMPKNLRRFKKAGLVREEKVLALPSSGSTGEP
                     MLRYRSAEIVVKRAQLRAELTAITSTDVLLTTRPINSGAGFSFHVTLPVLAGCKVVVH
                     EKFQRFKAAETIAKERVTVLNSVPLVFELLAAIPTSYPVDFSSLRLCMSGGAPLSRYV
                     REKFYQRYGIHLRQRYGGRHFSPACAWNYSDVPDAVGQKSGPFPLTVLSDDGRENGPD
                     QIGEVAFDYSKMAPLWKKALKDNPNRRGKFLHTGDLGRIDGAGNVFIVGRKSRFVKVA
                     SNRVEPAEVEAVLRSHPMIRDAVVFALNAGTPDEAIAAIVATAAKLTVSDVVSYCAAR
                     LQPYKCPRRIEFRRSLPHNAHGKVIRHVFDIANEQSPA"
     gene            10145..11026
                     /locus_tag="VMZ02_10710"
     CDS             10145..11026
                     /locus_tag="VMZ02_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72450.1"
                     /translation="MTRVPLIRLCHRVPLFMPLGRAIPVDWYYHWIKSVAPLIGRFSR
                     HAREQQHYIRLALAKSHSAEKQLDIARGNLIYRKWRVCLQVAWPNVAARSQQWVILQG
                     EENLARLARAGKGAILLCGHSFGFSGMAVRALAQRGYELVRAGAGREPARRRRAWGDG
                     DFQRWQYLGYDGDYWQRFRVLKRMHETLTRNAVLQLGIRGFPTGNPEARVESVYGPFF
                     LDRKVLGLIEVLQVPVLPCFVVCDQTGRAIIKIFPELVPSAKQIVSGFGSLYADYLRD
                     FPEHANIWKRVVQQKEF"
     gene            11028..11285
                     /locus_tag="VMZ02_10715"
     CDS             11028..11285
                     /locus_tag="VMZ02_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HUR72451.1"
                     /translation="MNRGEIHGCIEAIVRELYPQNDVAISENLPVLGPEGVLDSVLGL
                     QLLLKLEERFGIVVNDEEIDGENLSSLGSISRFVARKLAAN"
BASE COUNT         2399 a         3082 c         3324 g         2669 t
ORIGIN      
        1 gtgcggtcga agaaatcttt catgacgccg gacatcatcg cgaggttttc cggcgtggca
       61 aaaatgtaac cgccggcttg cagcacttca tcggcgccag cttcagctgc gtggatcaga
      121 ttcaccgcta tctccggctc gctctgcgcg ccactagcgg ccgcctgggc catctgataa
      181 gtgccgccgg tcatggaatg aaagacaatg agcagttttt tcattcggag gaatttaacc
      241 acaaagggca caaagttcac atagtaagga atgattcgag agatcggccg caaagaacgc
      301 aaagagctca aaagtagaaa tcggcaatct gaactaggcc gcagcctgct gtgcccttag
      361 cgaaggctcg cagcgagatt atcaataaag ccctcgtcgt ccagttcttt tagccagtgc
      421 agatcccaga gcgtcagcgg attgtcgatc gtcgggccgt attcttgggc ggcgatttcg
      481 tcggtgttga ggatcgcttc gatagtcggg tagggcctta tctgcaatag ttcagcgatg
      541 gagtcgactt ggcgccggag ttcgctaaga tcggtgatct tcatcaggcg cattggttcc
      601 tggacgatga tctccatcgc ttcttcgcgg cggtagatca gcaacgcgag agcgtggatc
      661 acggctttta ggtaatcctt tatcaacgtc gagttgtttc tgccgaactc ggagaggcaa
      721 gcttgggcga atagactgac gatctcagct tcgctggcgg ggaaggtttt gagtccggcc
      781 gccagggcgg cgggcaaata gatcggcgcc atgaagcagg ccacgcattc gccgctgacg
      841 attttgcgcc actgctgcca acggccgact tctttgtcag cgacaatgat ggttttcacc
      901 gagttctcaa gccccacttt gcgcagccag agcgtgatcc gatgaggccg gccgagatca
      961 cgcacggcca tggtcttgcc tttcagatcg tcgagcgaat taaccgactg tggcaccacc
     1021 aacttgagac cgcgttggat ttgtggcgcg cagaacagtg tcgctttttt gcctttcgcc
     1081 gcttcggtgt gaaggtattc gatgtgttcg atgatcacat cgcaggcatg atcgaataac
     1141 gccgcttcga actcgcgctg cggctcgatg tgcagcactt cgacgtcgat gcggtagtgc
     1201 cgttccgcca tcgctttgat gcagtagata accggtgtgc gatcgtggtc gcgataggcg
     1261 aggcgaacgg tgcgcatgat gagatttcta gtttgcctcc gacccgagcg aatagaccga
     1321 caatatctcc ggcagaggcg caaggacgca aagtaaaaaa tctattgcga actttacgag
     1381 cttggcgcct tggatgaatg tttctaaaat ggacctgccg gcacggctgg ccaggtttct
     1441 aacagctggc cgtagccggc gatgctgtag cgtcgtccaa gcttggaaag caccagccaa
     1501 agcatcgctg gattgctggc gacgatgggg agctttaact cgctttccaa cttgtcgatg
     1561 agcggaatgg gatcgagtag cgcgccctgg aagtagatcg cgtcgacgtt gggatattca
     1621 ctcaacgctt ttcgggtcat cgcttcgaca tcgctcgacg tgagcttttg cgcgtcggtg
     1681 tgggcgcgca gaatttgcgg cggatagaaa gactcgacgc caaattccgc cagatagtcg
     1741 tggtacatct tcttcaatcg gtcatccacg ggtgtcatga gcaaaatttt ccgcgccgaa
     1801 taagctttta gtgccgccac cgatgagcgc agggccaagg ccactggaac attcagtgcc
     1861 gttgagactt gctcccacat gccgggattt agcgcctcct tcggcgcgcc gctgatgatc
     1921 aggccattcc agccgttact ttcggccagc tcgcgcgatt gttcgatcag ggcatctacc
     1981 tttccctgag cgtcgtaaag cgaagtgtga gtgccagact cctgcaagaa cgtgaagtcg
     2041 atgtccgctg gaatgaattt tttgaagctt gcgtggtgcg gactcgatgg cgcgctggag
     2101 ccgagaaagc cgattttcat gggcgcggtt tgcgtcatat ggatgccaag agcggacgta
     2161 tgcgagacgc gcgacgagcg cgttgtaatt cttagtttct attgttccga ggcgagagag
     2221 ccgcgcacgc cccagtcttc tttgccggtg tcgtgaaaaa gtatgttaag cgactcgggt
     2281 ttgatattgg cgacttcggc gaagtccttg gaaatgcgtt tgaccagttc gtttttctgc
     2341 tccttgctct tgccgggaaa catatagata tggacgacgg gcatggtaac ctccatgtgg
     2401 actggatgag ttagccatat gcaaggaggc gccgaaaggc aactgaaaag tgctggcaat
     2461 cggcttgaca gagcccaatg gttcggatga tgatagattc aattcagctc ggagtgtaat
     2521 gatggaaaga acgctagtta cctggttggg tttttctttc gcggagcggc aactgttcct
     2581 gatcaagtca ggaatttttc tttcggtttg cggcgctacg ctgtttgtcg ctttagcgtc
     2641 gagctatccc gcggttcacg atacgctgca taacgttcgt cacgcccttg ccgtagttcc
     2701 ttgtcactaa gagcgctttg cgccgccttc tctttgtcgt gctcgctact cgtcggtgta
     2761 gctgcgagtg ctgaagagct aaagatttat ctaaagacta caccggcgct cgagtttttg
     2821 cggccgtttg ccgatccggt gactatgtcc cttcttgtta cccgggccga tggacggccg
     2881 ttggagcggg gcagcgtcgg catcgtcctt gacgcgccga agtcgggagc ttttttgtcc
     2941 acggatttcc ccgtcgtgga aaacagccgg ctgcttgagc tggccttgcc gctgcgccaa
     3001 ggacgcgccg gctggaaata tttatttccg attcgcggcg attatcgctt gtcagtcaac
     3061 gtggtcggtt ccgacggcac gaagacggcc aagaactttg tcattccggt cttggaagat
     3121 cgcacgaaat ggttgtcgct tgggttgttt tgcgccgcac tgtttattct cggtttcacg
     3181 gctgggagaa tttttaccgc tgccccagcg acaatgacaa cggcttggct gatattttct
     3241 tgtttggtca ccgcggtcag cggtcatgac gggccagtgt ctgtggaaaa tttcgcgccg
     3301 ccgcccaccg cgctggaaat tgccgccgct accgtcggca agccaacgca gctgcgctgg
     3361 cgcgcaccgg atggcggtgc ggcatcgaca ctgctctcga tatcgattac gcacctggaa
     3421 aaacacaaga tggtttttgc cgtagacaat gtgccggtgg acaaagacta tttgctcgat
     3481 ttccaattcc cagatggggc cgagtaccgg gtgaattcaa ttgcccagtt tcctggccgt
     3541 gggccgctgc gcaccgagca gctagtggcg gtgaccggcg tcgaaccgcc catggcggcg
     3601 caggtgccgg cactggcact ttttctagcc attatcgcgc ttggactcgg tgccggacgc
     3661 ttcagcaaga ttttggcggc tcggtaacgt caattctgca caaataaaaa agggcagccg
     3721 tcgaaccacg gctgcccttt ttcaatcaaa tctagtttgc ttattctgac acgtcgatga
     3781 gcacgccatc cgggtcggac atctggtact ggccgaagca gcaggtggcc agcaagttct
     3841 ggcgtacttc gcgctcggga tttttctccg ggcggatttc ttcggcggcg tagagatggg
     3901 catgcttcgc caggtcggct ttgaacgccg ccaggctttc aaccttgaat ccaatgtgat
     3961 cgagggccgg gcgctcgatg ccggaaccgg caaaatcgga aatcttccaa ggcatcaccg
     4021 ccagcgtcac cttgccgtcc gagaggtagt gattggcatc gccattgggc ttttccagtt
     4081 ccgttaactc gaaaacgtcg cgataaaatt cgccgcatag atcaggatcg acgcaacgca
     4141 agccgatgtg gccgacgcgc cgactgcgct cttcaatttt ctcggtatat agatcgcggc
     4201 ggttttccat attcagctgt gacagatcga agacattgcc gttgggatcg tgcatgctga
     4261 ttcccgcgaa gggacggttg cttggccggt gcaggacttg gatcttggga tatttttctt
     4321 ttaatcggcc aaagaccact tcgacgtctt ggacttcgaa gccgaaatga tcgaagcctg
     4381 cttgccgccc cggcgcgcgc ggattcaggt tcagtccgac gtagccgtcg gcgacgacta
     4441 cggctccgcc ggcgcgcgtt gggttggatc gcttcattcc aaagatgtca tgatagaagt
     4501 cgccctcgtg gctggcattg ctcgtgacga tagccatgtg tcttaattgt gcgaacatag
     4561 cggggctcct tttacgccga taagatgcta cccaacctag ttgaagcgca tgtagcttgt
     4621 caacctgata cgggagttct gaagtgatgg agaactggat cgcgattcga aatttgacgc
     4681 actttccaat actccatcac ttcattgagg gtcagacgtt gacgacgacc acgcctttgg
     4741 gctcgagata gtaatcgagc gcctcggggc catgctcgcg gccgacgccg ctcgacttca
     4801 ctccgccaaa gggcagctca tcataaccgt agtgcagcga attaacccac acgttgccag
     4861 cttgtagttt atcgatcgcc ttgttggcgt gcagcaagtt gcgcgtccag atcgacgagc
     4921 cgaggccgaa ttcggaagca ttggctttct ccagcgcttc gtcgagagtc ttgaaggtat
     4981 acacaggcaa cgctggaccg aagacttctt ccttggtcaa gcgcgagtct tcggggacgt
     5041 tggtcatcag agtcggtaga tagaaaaatc ccttgtcgaa ctcggcgccc ttgggacgct
     5101 cgccgccaaa cagcactttg ccgccgcgtg atttggcgtc ttcgacctgt tcttcgactt
     5161 cttgacgctg tgagccgata tggatcgggc ccatgcgggt ctctttgctg gtgccattgc
     5221 cgacagtttt tttcttcaag atttccacca gcccgccgat aaaattatcc gcgatggatt
     5281 cctggagaaa taagcgtttc acgcccagac acatttggcc gcagttaaaa tagcggccga
     5341 catcggccat cttaacggcc agatcgacgt tggcgtcttc catgacgatc atcggatcgc
     5401 taccgcccaa ctccaaggtc acgcgcttga tttctcgacc ggcgacttcc atgacatggc
     5461 ggcccaccgg cgtcgaaccg gtaaacgcga tgcggcgaac gcgcgggttg cgcagaagtt
     5521 cttcaccgac gctgccgccc ggacccgtga cgatgttgac ggtgcctttg ggcaattgct
     5581 tctgcgcttt gccttccccg aaggtggcct gagcgatgag ctcgatcact ttgatggtcg
     5641 ccagcggcgt agtgctggcc ggcttgatga tgatcgtgtt gccggcggcg agcgctgggc
     5701 cgagcttggt acccatcaga gttagcggaa agttccacgg cacgatgccg ccgcaaacac
     5761 cgatcggctg gcgcacgacc atgccgtagg cgttcttttg cggcagcggt acatgcgagc
     5821 cgcgcacctt ggaagccaga ccggcgtaga actcaaggcc gtgcacgaga tggtgaattt
     5881 ccaaaccggc ttcgaagagc gttttgcctt gttcttgggt gagcagttga gcgatctcag
     5941 aagtattttt cttgatcaac tcgcccgcgt tcatcaacgc actgccgcga tcttcgggcg
     6001 aaacatcggc ccagactttg aaggcgtctt cggcggcagc aatcgcttgt tgcacatcct
     6061 tgtcattgcc ttgcggtacg gtgtcgacgg ttgcaccggt ggcgggatta cgtacttcac
     6121 tggtgttctt ggttaccgaa tcgacggatt ccccgccgat aaacatcttg gccattttaa
     6181 ttcctccgtt gtgagtgcta actgaatata atgtttgcta tcacggcatc cgtagatgca
     6241 cgcgagctaa agtcgtcact ggtcagggtc agtggtcaga atggcaaagc cagctggaac
     6301 cggagctggc aaactatttc cccgtgaatt tcgccgtgcg cttttcgaga taggctttga
     6361 cgccttcctg gccgtcgtcg ctgccgaagg tttgcgccaa caactcgcgt tcgaacgcca
     6421 agccgtcggg gagcgatagt tccaggccgg tggtgatggc gcgtttgact ttgccgaccg
     6481 ccaaactgct cttgttgggc acgatgaact ggcgcgcgta atccatcact tggtccatga
     6541 agccttcttt gtcgtagatg taatggacca accccatctc gagtgcctcc tcgaaggcaa
     6601 tagttttacc ggtaaagcac agctccatgg cgcgcgcttt gccgaccaaa cgcggcaggc
     6661 gctgtgtacc gcccatcccg ggcatgacgc ctaaattgat ttctgccaag ccgactcggc
     6721 caccttcctt cttggcaatg cggatgtcac aggccatggc gatttccaag cctccgccaa
     6781 ccgcgtggcc attaatcgcg gcgatgatga tcttcggtgt gttttccagc cgcatgagca
     6841 cttcatggcc gtgcagcgcg aagttgtttt tgaaaccgag cgacttgctg gagagcatgt
     6901 tgatgtcggc gccggcggag aaaaatttct cgactttgcc ggttatgacg atggcgtgaa
     6961 catcgtcgtt gaagcgagcg tcgagcaccg ccgcgtccaa ctctttgagc agttcggtcg
     7021 tatagctgtt gaccgggggg cggttgattt ccaggactgc gatgccgctg tcgatacgcg
     7081 attcaatcat tgccatagtt cgtgcctcct catgcaaaat caactaaatg acttagctgg
     7141 catgcctgcg taagtcaagt tgccgacacc gaggaactag cggagatgtg tttgataaac
     7201 caaaggtttt tgccgatttg gagaagcgaa cgtgcgctcg ctgcgactct cgcggaataa
     7261 aattgcacga ttggaaagcg cctttgattg tgacatcact agggaccatc agatggccga
     7321 aaggataccc atttggcacg aaaagattct cacagcgagc agttgaaaaa gggtctcggt
     7381 gcgctcgccg ctgccggcat tgacgttgac ccgccaagcg tagcggcaat cgcgcagttg
     7441 cgcgcgcttt ggcgccaatc accggaaatg gatttggcga caattttttt actcggcaag
     7501 cttgccagcg tcgaagccgc cgagcttttg cgtgagctcg atcgcgatat cggcgacaaa
     7561 gaactgaaaa aagaagtcaa gcgctcactg ttcaagctca gccaaaaagg tttcgccgtg
     7621 ccgcaggagc aggcggccgc aaagcaaccg acgccagtat tcgagcggga aaatcagacc
     7681 gaagcatata tgtccgctgt cgatggcggc ggaggacgtc tgatttggct cgccaagccc
     7741 cagccgcagc gcggcttgca ggtgatccag gcgatggtgc atgacagcga gggactcgta
     7801 cgcttcggtg gcggccaaat gaagcgccga gaactgcgcg ctatggccga cgatatcaag
     7861 cagcagcatg gcgtcatcat gatcgccatt ccatgggaat ttgccgattg gatcgtctac
     7921 gaagggtatg agatggccaa ggcgcgcggg cagagcggat tggaagactt tcacgcgatc
     7981 cgttctgtga tcggcgcagg taaaccgaaa ccggtgacgc atccgatcta tgaattactt
     8041 ggcgctggtg attttcgcga gggcccctgg cgcgaacaat cacgccggct gctcgaggaa
     8101 ccggagctgc gttattgggt catcaccgac gagtgggtgc aggactgcct cacggcgctt
     8161 catgaagcgc agtcgagccc gttgattttg aatcaagtgc aaaaggaaga acggctcgcc
     8221 gccgtcgtgc gtgacgcggt aaaaacgttg tgtgccggcg accggggtcg agccttcaag
     8281 cggcgcatgg aagacatggc gctctacttc gccaaaactg agcgcagcga aaaggcgaga
     8341 ctcgcgctag ccgtggcaca acaagttgcc gagggcgatc ctgggccact ggatatttcg
     8401 ttcttgaccg gcctgatgca gaagagcttt gtctttttca tgtcacagct caaagctcag
     8461 cacgaagcgc aggagaaaga acgaaagtca tcgctgatca tcaagccgta agctattttc
     8521 tgacccgcct ccgcaagtgt ttcgcacgag tcccatgctg ggatccgcat gggcaaatgt
     8581 tgacatttaa gccagcttca ctataagcct gttgcgcgac cgatcgtcgg ctatttcgcc
     8641 atcggccaat aacatgattt atcgggaaat caaacgtcgt gcgaagcaag acccggataa
     8701 aataattatc attggggaac gaagaaacct cgactaccgc cagctcctca aagagatcga
     8761 cgcctgcgcg gcctattttc agtctgtcgg tatcaaagct caaagctcgg ttctgatcgg
     8821 agtcccgccc gccccggagt tctacgtcgc attcttcgca gcctgcgccg tcggcgcgac
     8881 ggcgattccg cttttgccct ccgcggcggg aattgcggcg cctgtgcgtg acagcaagcc
     8941 ggcaatggcg atcggcagtc gagcatttat caatgccgtt agatcgcagc ttccttccat
     9001 ttgtgacgaa ctgctttggg acgcgaaaaa cggcttgcac atgccgaaga atctccgacg
     9061 attcaaaaag gctggtcttg ttcgcgagga gaaagtcctc gcgctgccga gctcaggcag
     9121 cactggcgaa ccgatgctgc gctatcgctc cgctgagatc gtcgtaaaga gagctcaatt
     9181 gagagcggaa ctaacggcga tcacgagcac tgacgttctt ctgacgaccc gtccgatcaa
     9241 tagcggagcg ggtttcagtt ttcatgtcac cttacctgtg ctggccggtt gtaaggttgt
     9301 cgtgcacgaa aaattccaac gctttaaggc agccgaaaca atcgcaaagg aacgggtgac
     9361 ggtgctcaac agcgttccgc tggtttttga gctattggcc gcgattccca ccagctatcc
     9421 cgtcgacttt tcgtcgcttc gcctgtgcat gtccggagga gcgccgttgt ctcgctatgt
     9481 ccgggaaaag ttttatcagc gctatgggat tcatttacgg caacgctacg gcggccgcca
     9541 cttcagtccc gcatgtgcct ggaattattc ggacgttccc gatgctgtcg ggcagaagtc
     9601 cggcccgttt ccattgaccg tgctgagcga tgatggtcgc gaaaacggcc cagaccagat
     9661 tggcgaagtg gctttcgact attcgaaaat ggcgccgcta tggaagaagg ctttgaagga
     9721 caatccaaac cgccgcggca agttcctcca taccggtgat ttgggccgca tcgatggcgc
     9781 cggcaacgtt ttcatcgtcg ggcgcaaaag caggttcgta aaagttgcat cgaatcgtgt
     9841 cgagcctgca gaggttgaag ctgtcctacg ttcgcatccc atgatccggg acgccgtggt
     9901 ttttgcgctg aacgccggca ctccggacga agccatcgcg gcgatcgtcg cgaccgcggc
     9961 aaaactgacc gtgagtgatg ttgtaagcta ctgtgccgca cgattgcagc cctacaagtg
    10021 tccgcggcgg atcgagttcc gccgcagctt gccgcataac gcccatggta aagtcattcg
    10081 ccatgttttc gacatcgcta acgagcaatc cccagcgtaa ccagcagagc ccgttcagat
    10141 tgtcatgacc agagttccac tgattaggct gtgccatcgg gtaccgctct tcatgccgtt
    10201 gggccgagcc atccccgtgg attggtacta ccactggatc aaatcggtgg caccgctaat
    10261 tggccgtttt tctcgccacg cccgggagca gcaacactat attagacttg cattggccaa
    10321 gtcgcacagc gcagaaaagc agttggacat tgcgcgcggc aacttgatct atagaaagtg
    10381 gcgcgtctgt ctgcaggtgg cctggccgaa tgtcgccgcc cgatctcagc agtgggtgat
    10441 tttgcaggga gaagaaaatc ttgctcggct cgctcgtgca ggaaaaggag cgatcctcct
    10501 gtgcggtcat agcttcggtt ttagcggcat ggcggtgcgc gccctggcgc agcgcggata
    10561 tgagctggtt agggccggcg cagggcgcga accggcgcgc cggcggcgcg cgtggggcga
    10621 cggagatttt caacgttggc aatatctcgg gtatgacggc gactactggc agcgcttccg
    10681 ggtgcttaaa cgaatgcacg aaacactcac acgaaatgcc gtactacaac tcggcatcag
    10741 agggtttccg actggcaacc ccgaagcccg agttgaaagt gtttacgggc cgttctttct
    10801 cgaccggaaa gtactcgggc tgattgaagt cttgcaagtc cccgtgttgc cgtgttttgt
    10861 cgtttgcgat caaaccggac gcgcgatcat aaagattttt cccgaactgg tcccgtcggc
    10921 aaaacagatt gtctcgggtt tcggatctct ttacgcggat tatttgcggg attttccgga
    10981 gcacgcgaac atctggaaac gggttgtgca acaaaaggag ttttagcatg aaccgagggg
    11041 aaattcacgg ctgcatcgag gcaatcgtca gagagctata tccgcaaaat gacgtcgcca
    11101 tctctgagaa tttacccgtc ttgggtccgg agggggtgct cgattcggtg cttgggttgc
    11161 agttgttgct taagctcgag gagagattcg gtattgtcgt aaatgacgaa gaaatcgatg
    11221 gtgaaaatct gtcgagcctg ggcagcatca gccgctttgt cgcgcgcaaa ttggctgcga
    11281 actgacagga aggatttcgt ttcgttttag aagttcgctt acgaatcccg ccgactctgc
    11341 tggtcattgg cggaatcgat tttccagcgc aggggcgaat tatcttgatc gcgtgcagat
    11401 atcgagcgcc atgagcgcat agaccttggc cgcgtcgacc attatatcga tgtcgatctc
    11461 ttcgttgcgt ggac
//
LOCUS       DATLCH010000361         5215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10231889, whole genome shotgun sequence.
ACCESSION   DATLCH010000361 DATLCH010000000
VERSION     DATLCH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10231889"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1111)
                     /locus_tag="VMZ02_10720"
     CDS             complement(44..1111)
                     /locus_tag="VMZ02_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HUR72452.1"
                     /translation="MVLVLKNAQMENLLPMPEEIAAIELAFRELGQGKAMLAPRARLR
                     TPWKEEGGQYYFNNIMGLVPGMKSMALRIDSSFSKEIEVAGSKRRVYPGDFIGLVLLF
                     DMDTCNILAIMDDHYISAMRVGATSAVATNYLARKDAQVMALLGSGEQAKTQVTAHAC
                     VRKLTKVKVYSPTKANREKFAKDVSAETGIEVVPTNSAEEAIRGSDIVTAATNTVDPV
                     ILGKWVEPGMHLNSIVGGDGFLPRKELDDEAVVKANLIVVGYKPQIFLDKQAEFHDRL
                     ERGLVKAEDLHELGELVNGNCRGRQSEKEITFFKNNTGMGIQFAVTARKMYDKAKEKG
                     IGTELPLELFMTQRGDKLFSP"
     gene            complement(1259..1531)
                     /locus_tag="VMZ02_10725"
     CDS             complement(1259..1531)
                     /locus_tag="VMZ02_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HUR72453.1"
                     /translation="MTYIIAEPCIGTKDTACVDVCPVDCIHPTKNEAEEYEKETQLYI
                     DPEECIDCGACEPVCPVEAIFEEAATPDKWKNFIAINADWFKAKKG"
     gene            1681..2754
                     /locus_tag="VMZ02_10730"
     CDS             1681..2754
                     /locus_tag="VMZ02_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72454.1"
                     /translation="MLRLIIWILIAAASLVEADAPLTAATVDEAAKNLRSLSGVQRKT
                     FLEEGARKEGEVSWYTSMSLTDFPKIVGAFEKSYPYIKIKTNRLSQSSIMSRIETEAR
                     AGHYAVDLVGSAPMEMWELKQRNLSVPYLSPELKAFPSGSFDPQGYWSSTEVTPIVLA
                     FNTKLVSPDEAPKSYQDLLLPKWRGKMNFGSDEYSWYSVMLEGLGKAKGMDFMRALAR
                     QQLHIPGGSSIMRLQLMLAGESSMVIAARGRRATEYKEKGAPVDYRLLDPYPAEPNGF
                     ALMRRAAHPHASMLFVDWMLSEEGQTVLAQQVPRITLRKGVKQIPRHQELYKKEFVFV
                     NPAYLGANLTDLIASYQQVFNLR"
     gene            2995..3438
                     /gene="ybeY"
                     /locus_tag="VMZ02_10735"
     CDS             2995..3438
                     /gene="ybeY"
                     /locus_tag="VMZ02_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HUR72455.1"
                     /translation="MTVEITRLGVGTSYPTAALRSIAETLLRAVKQSRAELSIALVGD
                     REMRPLNSKYRKKRKTTDVLSFFVEGQPPPGVKILGDVVISVEQARRQAQACGRTLKS
                     EMVTLLIHGVLHLLGYDHEKSARQAKIMFAYEQKLYAHLCDRGLL"
     gene            3533..4591
                     /gene="prfB"
                     /locus_tag="VMZ02_10740"
     CDS             3533..4591
                     /gene="prfB"
                     /locus_tag="VMZ02_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HUR72456.1"
                     /translation="MSSCGGGFDVDSKQKRVEELNQITAQADFWNDADKAQTVLKEQS
                     ALKAVLANWEKHQVDLEEARFFLDVAKDEKSEEALGEAAGKVAAITKEMAQTELTQLL
                     GGPDDRRNAIVTLHPGAGGTEAQDWAEILLRMYLCWCDRRGFRKEILDYQPGEEAGVK
                     SVTFNVTGDYAFGYMKAEAGIHRLVRISPFDANSRRHTSFASVFVYPEVDDDIKIEIN
                     DDDLRVDTYRSSGAGDQHVNKTDSAVRLTHLPTGIVVACQNERSQHKNRAMAMKILKS
                     RLYELELVKQKEKMESYHKTKKDIAWGSQIRSYVLAPYRLVKDHRTNVEVGNADAVLD
                     GDLDQFIEAYLMMADEPA"
     gene            4763..>5215
                     /locus_tag="VMZ02_10745"
     CDS             4763..>5215
                     /locus_tag="VMZ02_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUR72457.1"
                     /translation="MKTPIAQMFEDEGHKTDVEAAVLQRYGSAAQEVDSCLCLPVSYD
                     RALLKAIPDEILERDYGCGDPSQYVRSGETVLDLGSGSGKACYIISQIVGAAGKVIGV
                     DFNPPMLELARKYQKSIGDQLGFHNVEFRRGKIQDLRTDLELIEQYLTA"
BASE COUNT         1179 a         1456 c         1446 g         1134 t
ORIGIN      
        1 aaaccggctt agctgcatta atgttgcgga tcacaatcag caatcacggg gaaaacaatt
       61 tatcgccgcg ctgggtcata aacaattcta gcggcagttc ggtgccgatg cctttttctt
      121 tggctttgtc gtacatcttc cgggcggtga ccgcgaattg gatgcccatg ccggtgttgt
      181 ttttgaaaaa agtaatttcc ttttcactct ggcggccgcg gcaattgccg ttgaccaatt
      241 caccgagctc atgcaggtct tcggctttga ccagaccacg ctccaagcga tcgtgaaact
      301 cggcctgctt gtcgagaaag atctgtggtt tgtagccgac gacgatcaaa ttggctttga
      361 cgaccgcctc gtcgtccaac tcttttcgcg gcaggaagcc atcaccgccg acgatgctgt
      421 tgagatgcat gccgggttcg acccatttgc cgaggatgac cggatcgacg gtattggtcg
      481 ctgccgtgac gatgtcgctg ccgcgaattg cttcttcggc tgagtttgtc ggcaccacct
      541 cgatgccggt ttccgcgctg acgtccttgg caaatttttc gcgattcgcc ttggtcgggc
      601 tgtagacttt gaccttggtc aatttgcgca cgcaggcatg cgccgtgact tgagtcttgg
      661 cctgttcgcc cgagcccaaa agcgccataa cttgagcgtc cttgcgcgcc aggtaattgg
      721 tcgccacggc gctggtggcg ccgacgcgca tggccgagat gtaatgatca tccatgatcg
      781 ccaagatgtt gcaggtatcc atatcgaaga gcagcacgag accgatgaaa tcgccgggat
      841 agacgcgccg cttgctgcct gcgacttcaa tttccttgga aaaactcgaa tcgatgcgca
      901 acgccatcga tttcatgccc ggcaccaggc ccatgatatt attgaaatag tattggccgc
      961 cttcctcttt ccacggcgtg cgcaggcgcg cgcgcggcgc aagcatcgcc ttaccctgac
     1021 ccaactcgcg aaatgccagc tcgatggccg cgatttcctc cggcattggc agcaagtttt
     1081 ccatctgtgc gtttttgagt actaggacca tgcgatctcc ttggctaggt tctgcgtaca
     1141 tcagagtcta aaggtgagtc aaggcgcgca acgtcagcaa gtcgggtgca aaaaaaaggg
     1201 ctgagaaacc ttctcagccc ttgtgaaacg aaacagtgcg aatcaggttg cttagaggtc
     1261 agcctttttt ggctttgaac cagtcggcat tgatggcgat gaagtttttc catttatccg
     1321 gcgttgccgc ttcttcgaaa attgcctcga ccgggcagac aggttcgcat gcgccgcagt
     1381 cgatgcattc ttccggatca atataaagct gggtctcttt ttcgtattcc tcggcttcgt
     1441 tcttggtcgg gtgaatacaa tcaaccgggc agacatcgac gcaagcggtg tctttggtcc
     1501 cgatgcaggg ttcagcgata atgtaggtca tctattcctc ctagatcaaa cttcaaccta
     1561 gttagcaaat cgaattcggc aagtcaattt aacggccgtg tatgaccggt tgattcaccg
     1621 ccttgaccgc ctccagcgct gatgttagat gcaacttact cgagattggc ggaaaatttt
     1681 atgcttcggc tgataatatg gatcctgatt gcggcggcaa gcctcgttga agcggatgct
     1741 ccgttaacgg ctgcgacggt cgacgaggct gcgaaaaatt tgcgcagcct gagcggggtg
     1801 cagcgaaaga cgtttttaga ggagggcgcg cgcaaggagg gcgaggtgag ctggtacacc
     1861 tcgatgagct tgacggactt ccccaagatc gtcggcgcgt ttgaaaaaag ctacccctac
     1921 ataaaaatca agaccaaccg cttatcgcaa tcatcgatca tgtcgaggat cgaaaccgaa
     1981 gcgcgtgccg gacactatgc cgtcgacctg gtcggctcgg cgccgatgga aatgtgggaa
     2041 ctcaagcagc gtaatttgtc ggtgccgtat ctttcgccgg agctgaaggc gttcccctcc
     2101 ggttccttcg atcctcaggg ttattggtct tccactgaag tgacgcccat cgtcctggcg
     2161 ttcaacacca aattagtctc tcccgatgag gcgcccaaga gttatcagga cctgttactg
     2221 cccaagtggc gcggcaagat gaatttcggc agtgacgaat actcctggta ttcggtcatg
     2281 ctcgagggcc tgggcaaagc caaagggatg gatttcatgc gcgccctggc gcgccagcag
     2341 ctgcatatac ccggcggcag cagcatcatg cgcttgcagt tgatgcttgc cggtgagtcg
     2401 tcgatggtga tcgccgcgcg cggccggcgc gccaccgaat acaaagaaaa aggcgcgccg
     2461 gtggactatc gcttgcttga tccctatccg gctgagccca acggctttgc cttgatgcgc
     2521 cgggcggcgc atccgcacgc ttccatgctg tttgtcgact ggatgctttc cgaagaaggc
     2581 cagaccgtgc tggcgcagca agtgccgcgc atcacattgc gcaagggcgt aaagcaaatc
     2641 ccccggcatc aggagcttta caaaaaagaa tttgtcttcg tcaatccagc gtatctaggc
     2701 gccaatttga ccgacctgat cgcgtcctat cagcaggttt ttaatttacg ctaggcggca
     2761 cggtagtgga atgtaacatc aaattagcgt tcaaatcttt tcggagccct tcgacctagc
     2821 tcagggcgaa cggtcaccgg aattccgttc gtggtgagct tgtcgaacca tgaacggttg
     2881 acgctaatca acggttacat attactagtt accctttgat aaaggcttgg cagcgtggcg
     2941 gagagagatt tcgcgctgcg ctcgaaatga cggaattcgg ctgcttctcc actcatgacc
     3001 gttgaaatta cccggttggg cgttgggacc tcgtatccga ccgcagcgct gcgcagcatc
     3061 gccgagactc tcctgcgcgc cgtcaagcaa agccgcgccg aactcagcat cgcgctggtg
     3121 ggcgaccggg agatgcgccc cctcaacagt aaataccgca agaaaaggaa aaccaccgat
     3181 gtgctgtcct tcttcgtcga aggccagccg ccgcccggcg taaaaatact tggcgacgtg
     3241 gtcatctccg tcgaacaggc gcgacgccaa gcgcaagcct gcggcagaac tctcaagagt
     3301 gagatggtaa ccttgttgat ccacggcgtt ctgcatctgc tcggttacga ccatgaaaaa
     3361 tccgcgcgac aagcgaaaat catgttcgcc tacgaacaga aactttatgc ccacctttgt
     3421 gaccgcgggt tattgtagct atactgccgg caacatcatt ttctgactat tcacaagaaa
     3481 gtacccatga tcgaagaaac cacggaacaa cttagccacc tcgaagggcg acttgagcag
     3541 ttgcggaggc ggctttgacg tcgacagcaa gcagaagcgt gtcgaggagc tgaaccaaat
     3601 caccgcccag gccgatttct ggaacgacgc cgacaaagcc caaaccgttc tcaaagaaca
     3661 gtccgcccta aaagccgttc tcgccaactg ggaaaaacat caagtcgatc ttgaagaggc
     3721 acggtttttt ctcgacgtcg ccaaagacga gaaaagtgaa gaggcgctcg gcgaggccgc
     3781 cggcaaagtt gcagcgatca ccaaggagat ggcacagacc gagctgaccc aactgctcgg
     3841 cggtcccgac gaccggcgta acgccatcgt cacgcttcat cccggcgccg gcggcaccga
     3901 ggcgcaagac tgggcggaga ttctgttgcg catgtatctg tgctggtgcg accggcgcgg
     3961 cttccgcaag gagattcttg actatcagcc cggcgaggaa gccggagtca aaagcgtcac
     4021 cttcaacgtc accggcgatt atgcattcgg ctatatgaaa gccgaagcgg ggattcatcg
     4081 gctggtgcgc atttcgccgt tcgacgccaa ctcgcgccgg catacttcgt tcgcttcggt
     4141 cttcgtctat cccgaggtcg acgacgatat taaaatcgag atcaacgacg atgacctgcg
     4201 cgtcgatacg taccggtcga gcggcgccgg cgaccagcat gtcaacaaaa ccgactcggc
     4261 cgtgcgactg acccatctgc cgaccgggat cgtcgtcgcg tgtcagaatg agcgctcgca
     4321 gcataaaaac cgggcgatgg cgatgaagat tcttaagtcg cggctctacg aattggaact
     4381 cgtaaagcaa aaagaaaaga tggaaagcta ccacaagact aagaaagaca tcgcctgggg
     4441 cagccagatc cgctcctacg tgctcgctcc ctaccgcctg gtcaaagatc accgcaccaa
     4501 cgtcgaggtc ggcaatgccg acgccgttct cgacggcgat ctcgatcagt tcatcgaagc
     4561 ctacttaatg atggccgatg agccggctta atgccctgag aagcgatgcc gagcggatga
     4621 cgattcagct cggtcgcggc gcgcgaaaat gtcctgccat gaagcgttgc attaattcct
     4681 acagagcggc gccgggtgac tacgttcgct tctttgacac tgaagtgggt ccgccatcta
     4741 acacatgacc gcgggaggat ttgtgaaaac gccaatcgca cagatgttcg aagacgaagg
     4801 acataaaacc gatgtcgaag ccgcggtgtt gcagcgctac gggagcgcgg cgcaggaagt
     4861 cgactcctgc ctatgtttac cggtgagcta cgatcgcgcc ttgctgaaag cgattccgga
     4921 cgagatcctt gaaagggact acggctgcgg cgatccgtca cagtatgttc gttctgggga
     4981 aaccgtgctc gatcttggct cgggcagcgg caaggcctgc tacatcattt cccaaatcgt
     5041 cggcgccgcc ggcaaagtca ttggcgtcga cttcaacccg ccgatgctgg agctggcgcg
     5101 caaatatcag aagtctatcg gcgaccagct gggatttcac aacgtcgaat tccgccgcgg
     5161 caaaattcaa gacttgcgta ccgacttgga gctcatcgag caatatctca cggcc
//
LOCUS       DATLCH010000362        15557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_3720642, whole genome shotgun sequence.
ACCESSION   DATLCH010000362 DATLCH010000000
VERSION     DATLCH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 15557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15557
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720642"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VMZ02_10750"
     CDS             <1..451
                     /locus_tag="VMZ02_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008671139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HUR72458.1"
                     /translation="DLLHSRDFLYLGRGINYPIALEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDEKMPVVVLAPRDRHFDKTMSNLKEVESRGGKIVVVTDDAKTAREIPAFRV
                     LTLPKGSHYLTPIVMTIPLQLLAYYIAVLGGKDVDQPRNLAKSVTVE"
     gene            451..1719
                     /locus_tag="VMZ02_10755"
     CDS             451..1719
                     /locus_tag="VMZ02_10755"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72459.1"
                     /translation="MTHRLPGPTQLAITLSSLKHRNFRLLFCGTSLSHVGDFIQAMAQ
                     SWLVWQMTKSPFLLGIVGFAQALPRLLLGAVGGAIVDRVERRRLLLCTQFFAMAQALT
                     FWVLVQFGWVEFWHVLVLVLFLGTVNSLNQTARHSLINNIVPREDLMNAIALNSSLAN
                     LAKIVGPSLGGVLIGVIGIAGCLLVNAISFFAIIVTLVIMEIPPGKAKLDKEASFWQE
                     VSEGYRFLRSERRLLAVILLTYGVAMVGTPYSRFLPVFATDVLHAGPTTFGLLLASPG
                     IGAVFAGLGIASLGRLRRRLHFVVTAVMAFSISLMLFSFSRSIYLSMLFLVFVGASNI
                     AFRAVANSIVQMETPPQYLGRMLSLFFMDKGLWSFGTLFIGSVAHVIGTPRAITVSGA
                     FCAIAASILLYQQRQARGGPLAEQSDRRVA"
     gene            complement(1699..2688)
                     /locus_tag="VMZ02_10760"
     CDS             complement(1699..2688)
                     /locus_tag="VMZ02_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72460.1"
                     /translation="MSEIRKDPLTGRGVIIAPERAPRTKQLGADVNINSVQVCPFCPG
                     NENLTPPELWADRPGDSPPDQPGWSVRVVPNKYPAVSSGKAPERQTRDRAAAIGVHEV
                     IIESATHVANLAALDQSQFARVVYAYRQRLRAARENTCWRFRLLFKNHGERAGATFEH
                     THAQLVALPFVPVDVEVELASAGQYYQRNGHCYFCALLRREMHARARMVIDNAEFIAL
                     CPSAPRFAFETWILPRIHTPAFEDTDDVSLAAFAGIARKVIAALDRLETNPPFNYFIQ
                     SLSLDEREREHSHWQFRLLPQFSRAAGFEWGSGLHINPVAPEDAARILRDAAI"
     gene            complement(2692..3306)
                     /locus_tag="VMZ02_10765"
     CDS             complement(2692..3306)
                     /locus_tag="VMZ02_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HUR72461.1"
                     /translation="MAYIPQDRFFRKAKQDGYRSRAAYKLLELQERFHLMNAGDKVVD
                     LGAAPGGWLQVAAKLVGPNGKVVGVDLQPIQPFRDSNTVVLRGDVLTGETQGRIKELL
                     GGKAHCVLSDMAPKLTGIRDADMARCQELNRLALQVGKELLRPGGVLLVKSFVSDDLQ
                     SYSAELKDFFATVQRTKPEASRQGSSEFYFYAKNYQPQTITAAP"
     gene            complement(3403..4164)
                     /locus_tag="VMZ02_10770"
     CDS             complement(3403..4164)
                     /locus_tag="VMZ02_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HUR72462.1"
                     /translation="MKRRTVQAVTLAMAIGLAGQASFAAPDEYDDSQSHPLRVLAYLA
                     YPVGWLAEWTIFRPFHLLVSGTRAQEAFFGHTPHPTVLAEPQTLHDYGVAKKVPVHTV
                     RVAPPAPPPVPMPEHVKIVEVPVEKTVIQVQEKVVEVERLVFTPVAFNFDSAYLTDLG
                     KGQVYLAAQRLRERGNLTIVVEGHTDNRGDDSYNQQLGLQRAQTVMNELASLGIDQSR
                     MSAVSTGESKPLIEHDTAWARAINRRVEFQVRTAK"
     gene            4327..5088
                     /locus_tag="VMZ02_10775"
     CDS             4327..5088
                     /locus_tag="VMZ02_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HUR72463.1"
                     /translation="MNVNVDKKDRLITITWDQPPLNVLDLSLLRELDAILLGCAAEPA
                     VDVIVLRGQGSKAFSAGVDIKDHTPKKVPEMLDVVHGVIRKLLSVPQITVAAVQGACL
                     GGGCEVASVCDFVVATDDSFFATPEILVGCYPPVALARFATLIGYHRAAEMILTGRRF
                     TAHEAQGMGLINRVFADAELETGVESLVGELLSKSGAVMRLTVKALRALSLGDFDAAL
                     RRSEQIYCDELLSTADVEEGVQAFLQKRPPQWRHR"
     gene            5164..5934
                     /locus_tag="VMZ02_10780"
     CDS             5164..5934
                     /locus_tag="VMZ02_10780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HUR72464.1"
                     /translation="MAEFEIKIATDAQEIVEAQRLRFQVFNLELTKGLQSSYQSGLDV
                     DQFDPFCNHLIVRDTKSKEVVGTYRMMLGAQARQHLGFYSEKEFDLTRIKKLDGELLE
                     LGRSCARKDYRDRALLPLMWEAIADYVRANRVRYLFGCGSLYTTDVAEISAIFALLRN
                     KYYAPDSCRVEPVAGCRFEGLPDLQVCSAEEAALFQKLPSLIKGYLRIGALVCGPPAL
                     DIEFGTADFFLLLDFGSLKEAFLKRLGMGDLRGNEAPA"
     gene            5918..6757
                     /locus_tag="VMZ02_10785"
     CDS             5918..6757
                     /locus_tag="VMZ02_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HUR72465.1"
                     /translation="MKRLLKLAAFLSLWVFFFALVALVHLWISVLGMPNRWKIISRIN
                     RTYTLLLRSILNIKLTIVGNEGQLERGGYVIIANHVGYIDGIVLGSIFPIVFVSKKEV
                     RSWPIVGQWNILCGTVFIDRQHKEQVGLLVQEMSRKLKHEANILLFPEGTSTNGERML
                     PFQTVPLAAPLRSRSVIVPTTLAYKSLDDVPINAVNRDLIYWYGDMDFVSHFWKLLAL
                     RSIEVLVTIQPKVECFRYSDNSAGRKKLAEDCYNRVLGRITESSLDQDDEIEEDGEPL
                     VRA"
     gene            complement(6743..7195)
                     /locus_tag="VMZ02_10790"
     CDS             complement(6743..7195)
                     /locus_tag="VMZ02_10790"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HUR72466.1"
                     /translation="MKFSQTAQIPVAREPLWDFLMDVPKVAKSLPGVESVSKIDETTY
                     EGALTVRVGPIKLNLQGKIILEEQDKTNWRAALRAEAKDRMAAGAVNGKTIMNLKEID
                     ANRTELLVETDVNILGKIGEFGQPIIRKKADAMLKEFVENIKKQLSAN"
     gene            complement(7192..8127)
                     /locus_tag="VMZ02_10795"
     CDS             complement(7192..8127)
                     /locus_tag="VMZ02_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HUR72467.1"
                     /translation="MIPFEYRTPKNLKEVHATLKEFGSDAKLIAGGTALVIMMKQRLV
                     RPTCLVSLQSVRGLNGIEVKDGGLSIGGLATHRAVESSSLVRRRIPMLAETYHHVATL
                     RVRNMATVGGGLAHADPNQDPPPSLIALGAKMKATSPMGSRTISLDEFFKDYYETVLN
                     PDEIITEVMVPKQAPNTGAAYIKFLPRTADDYATVSAAAVLTLDKSMKTIADVRIALG
                     SVGVTPIRATEAEAVLRGQPLKAEAFAEAGEKAKQAVDPISDFRGSAAYKTDMAAVFV
                     RRALEKALADIRQQAKAKAAPKPAKKKIVKKGKKR"
     gene            complement(8141..8608)
                     /locus_tag="VMZ02_10800"
     CDS             complement(8141..8608)
                     /locus_tag="VMZ02_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HUR72468.1"
                     /translation="MTKKIKFRVNGKRHEVDVPTNRMLIDCLRYDLGLTGTKEGCSVG
                     VCGACTVLMDGDMISSCLTLAVMADGHDLTTVEGLAKNGELNPVQKSFIQYGGFQCGI
                     CTPGQVVAATALLKENPKPNEDEIKDWMMGNLCRCTGYYKIIESIQKAHQFAK"
     gene            complement(8828..9937)
                     /locus_tag="VMZ02_10805"
     CDS             complement(8828..9937)
                     /locus_tag="VMZ02_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HUR72469.1"
                     /translation="MKSRAAVAWESGKPLTIEEVEVGAPKTGEVLLKIVASGVCHTDA
                     YTLSGKDPEGLFPAIMGHEGGATVMEVGANVASVKPGDHVIPLYIPECGECKFCKSGR
                     TNLCGAIRLSQGKGVMPDGTSRFSLGGKEILHYMGTSTFSEYTVLPEMAVAKINPAAP
                     LDKVCLLGCGVTTGIGAVLNTAKVRPGSSAAVFGLGGIGLSVIQGLVMAKAERIIAVD
                     LNEKKFEMAKQLGATDFIVPQADGITIQQAIVDLTDGGVDYSFECIGNVNTMRAALEC
                     CHKGWGESTIIGVAAAGQEIATRPFQLVTGRVWRGTAFGGVKGRSQLPGYVERYLRGE
                     IKVDPMITHVLPLDRINEAFDLMHEGQSIRSVIKF"
     gene            complement(10414..11991)
                     /locus_tag="VMZ02_10810"
     CDS             complement(10414..11991)
                     /locus_tag="VMZ02_10810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR72470.1"
                     /translation="MNLIPAKRRTLKVNLFALFFQLTLISGYTSIAVSQDESELRRTI
                     QSTPDPRGQAKLYKQLGDQLVRQDRIDDAAEPYIRALNLDAAGFSAAERVQMAIYLSW
                     TNHLAEARAELANVVALEPQNIPARTHLARVLAWSGELAAAIIEADRVLAQSPQHKDA
                     LLVKADALQWQGRNAESIPIYQQIVARDDDFDARAGLARAQLAVRNRVAALESRDTLK
                     PGTPKQQRELARLNLEIDDEIRPTLDGRYNYYSDSDQNRVDRYNLAATYWVGNQKLGM
                     DYRHTEASDPTRANRAEDFSAKLYSRLSDVVSAGVAIGFSQVADRHTSTFPTGQLRID
                     AKLFDGSIGTGVTREVLSDTAELVQNRIRMTVGSFNLQQNLTERLSVQGRYNYKSLSD
                     GNHANELQLATQYSVYLKPRVVIGHRFRLLDFQKQTGSGYFDPNNYIANRGFTSMYFD
                     NRTYYAYVEGYLGYETFRRNGIASNNFIHGGSGSIGIKPLENLSLEINVEGGNFAAGS
                     ASGFTYFNAGPRVVYRF"
     gene            complement(12008..13246)
                     /locus_tag="VMZ02_10815"
     CDS             complement(12008..13246)
                     /locus_tag="VMZ02_10815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HUR72471.1"
                     /translation="MLWVTVSWYLAQSWIGELSPITGYFTAVGIIFFIALLPGFLNAH
                     ILSSVVLDRPPDLPRLLEYPPISLLIAAYNEEENIAETFRGIAAQDYSGTVEIIVVDD
                     GSSDNTVGVIEALELANVKIVRGNHGGKAMALNQGLEYVAHDILVCIDADTFLHPQAL
                     RRIVTRFLTDPQDTAAVAGCVLVKNSRATFMTRLQEWDYFTGIASAKRQQSLYQGTLV
                     AQGAFSAFHSKVVKAHKGWPSVIGEDIVMTWALIKSGWRIGFEPTAIGFTSAPTSFIG
                     FYRQRKRWARGMIEGLKQHGQLVWSTSRLAAFFVAIDFIIPFIDLFYAFVFLPGVVLA
                     LLGYRFIVGPMTVLVMPLAFLIVLAMYKKQKAVFDVLELRVRQNFAGFFVYMLIYQAI
                     MSPICVIGYAQELLGTKKRW"
     gene            13817..14434
                     /locus_tag="VMZ02_10820"
     CDS             13817..14434
                     /locus_tag="VMZ02_10820"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HUR72472.1"
                     /translation="MFLEMRTYVLKPGMTNNFVEGFAAGLPARLQISKLLGLFCSEVG
                     GLNRVTHVWPYESFEDRERIGNEARKTGKWPPKTQEFIVTQENKIIQLAPFSPPLPEK
                     KLGEIYEFRTYTYQPGTMPLVMERFGKVIPERTKISPLVFAGQTMIGPLNQFIHVWAY
                     KDAGERERLRGEAAKKVAGWPPATREFMVAQENTIMTPAACAPLN"
     gene            14604..>15557
                     /locus_tag="VMZ02_10825"
     CDS             14604..>15557
                     /locus_tag="VMZ02_10825"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HUR72473.1"
                     /translation="MFYKSLIRPVLFRKDPEESHDAVLRMLARNEWLYGTLEEFYKIE
                     DERLEVKIGPLAFANPVGLAGGFDKNALAPKMISAFGFGFMEVGAITAQAQPGNPKPR
                     LYRLPEDAALINRLGFNNEGAEAIAIRLDCLRARGGRPKIPLGMNIGRTKIVETKDAV
                     ADFLSCFDALFPHGDFFTVNVSSPNTPNLRELQDKNLLSELLSALQEKNTQLAKQAKI
                     DPKPVFVKIAPDMEFDQVDEIIEVVDQEKLTGIVATNATAFKREGLKSANGREPGGLS
                     GRPITAMVTHFISHIYRNTRGRLPVIGVGGIFNAEDAYEKIK"
BASE COUNT         3376 a         4055 c         4310 g         3816 t
ORIGIN      
        1 ggatcttttg cacagccgcg attttctcta tctcggccgg ggtatcaatt atcccatcgc
       61 cctagagggg gcgcttaaat taaaagagat atcctacatc catgccgagg gttatcccgc
      121 tggtgagatg aagcatggac cgattgcctt gattgatgaa aagatgccgg tggtcgttct
      181 tgcgccgcgc gaccgccatt tcgacaaaac catgagcaac ctgaaggaag tcgagtcgcg
      241 cggcggtaaa atcgttgtcg taaccgacga cgcaaaaacc gcccgcgaga ttcccgcgtt
      301 tcgggtcttg acgctgccca aaggttcgca ttatctaacg ccgatcgtca tgaccatacc
      361 gctgcaattg ctggcctatt atatcgccgt tctcggtggt aaagacgttg atcaaccgcg
      421 caatcttgcc aaaagcgtta cggttgaata atgacccatc ggttaccagg gccgacgcaa
      481 ctcgcaataa cgcttagttc gctcaagcac cgaaatttcc gtttgctgtt ctgcggcacc
      541 agcctctccc atgtcggtga tttcattcaa gccatggcgc agagctggct cgtctggcag
      601 atgaccaaat caccctttct tctcgggatc gtcggctttg cccaggcttt gccgcgtttg
      661 cttttgggcg ccgtcggcgg agccatcgtc gaccgggttg aacggcgccg gctgctgtta
      721 tgcacgcaat ttttcgccat ggctcaagcg ttgactttct gggtcttggt tcaattcggc
      781 tgggtcgagt tttggcacgt gctggtgctg gtgctttttc tcggcacggt gaattcactt
      841 aaccagactg cgcgacattc gcttatcaac aacatcgtcc cgcgggagga tttgatgaat
      901 gccatcgcgc tgaattcttc actggccaat ttggctaaaa ttgtcgggcc gtccttgggc
      961 ggcgtgctga tcggcgttat tggcattgcc ggctgtttgt tggttaatgc gattagtttc
     1021 tttgcgatca tcgtcacctt ggtaatcatg gaaattcctc caggaaaagc caagctcgat
     1081 aaagaagcat ccttttggca ggaggtttcc gaaggttatc gttttctccg cagcgagcgg
     1141 cgcttgttag cggtcattct cctgacttat ggcgttgcga tggtaggcac gccctattcg
     1201 cgttttctcc cggtgttcgc taccgatgtg ctccatgccg gtccgacgac atttggtttg
     1261 ttgctcgctt cacccggaat cggtgcagtc ttcgcggggc tcgggatcgc gtcgcttggg
     1321 cgattgcgaa ggcggcttca tttcgttgtc acggcggtga tggcattctc aatctcgctg
     1381 atgctctttt ctttttcgcg ctcgatttat ctttcgatgt tatttttagt ctttgtcggc
     1441 gctagcaaca tagcgtttcg cgccgtcgcc aactccatcg tccaaatgga aacgccgcca
     1501 cagtatctcg gtcgcatgct tagcctattt tttatggata aaggtttatg gtctttcggc
     1561 acgttgttca tcggcagtgt cgcgcacgtg atcggtacgc ccagggcgat caccgtttcc
     1621 ggtgcgttct gtgcgatcgc ggcatcgatt ttactttacc aacaacggca ggcgcgcggc
     1681 ggcccgctcg ctgaacagtc agatcgccgc gtcgcgtaat atccgggcgg catcttcagg
     1741 tgctaccggg ttgatgtgca gacccgagcc ccattcgaaa ccggcggctc gtgaaaattg
     1801 cgggagtaag cggaattgcc aatgggagtg ctctcgctca cgttcatcca aggacagcga
     1861 ctgaatgaaa taattgaacg gtggatttgt ctctaggcgg tccaacgcgg caattacttt
     1921 ccgtgcaatt cctgcgaaag cagcgagact tacgtcatcg gtgtcttcga aagctggcgt
     1981 gtgaatccgc ggtagtatcc aagtttcgaa cgcgaagcgc ggcgcggagg ggcaaagcgc
     2041 aatgaattct gcattgtcga tgaccatgcg tgcgcgtgcg tgcatttcgc gtcgaagcag
     2101 cgcgcaaaaa taacagtgcc cattgcgctg atagtattgg ccggcgctgg cgagttcaac
     2161 ttccacatcg accggcacga aaggtaatgc caccaactgc gcatgcgtat gttcgaaggt
     2221 ggcgccagca cgttcgccgt gatttttgaa aagtaacctg aagcgccaac acgtattctc
     2281 tctcgcggcg cgtaaccgct ggcgataggc ataaacaacc cgggcgaact gggattggtc
     2341 cagtgctgcc aagttagcca catgagttgc actttcaatg atgacctcgt gcacaccgat
     2401 ggccgctgct cgatctcgtg tttggcgctc tggcgcttta ccagaggaga cagcggggta
     2461 cttattgggc actactcgga cggaccagcc gggttgatcg ggtggcgaat cgcctgggcg
     2521 gtccgcccat aactcgggcg gcgttaagtt ttcgttgccg gggcagaatg ggcagacttg
     2581 gactgagttt atgttgacat cggcaccgag ttgtttagtg cgtggcgccc gttcgggggc
     2641 gatgatcacg ccacgtccgg tgagcgggtc ttttctaatt tccgacatta ctcatggcgc
     2701 ggccgtgata gtctgtggct gataattctt tgcgtagaag tagaactcgg atgagccctg
     2761 gcggctagcc tcgggcttag tacgttggac cgtcgcgaaa aaatctttca gctcagcgct
     2821 atagctctgt aagtcgtcgc tgacgaaact cttgaccaat aatacaccac ctgggcggag
     2881 tagttctttg ccgacttgca gtgccagtcg attgagttct tggcagcgcg ccatgtcggc
     2941 gtcacggatg ccggtgagtt tcggtgccat atccgagagc acacagtgcg ccttgccgcc
     3001 tagcagttct ttaatcctgc cttgagtttc accggtcaaa acatcgcctc gaagcactac
     3061 cgtgttgcta tcgcgaaaag gctggatcgg ctgcaaatct acgccaacga ctttgccgtt
     3121 ggggcctacg agtttcgcgg cgacttgtaa ccatccgccc ggcgctgcgc caaggtcgac
     3181 cactttgtcg ccagcattca tcaagtgaaa ccgctcttgc agctcgagca gtttgtaggc
     3241 ggcgcgcgag cggtagccgt cctgtttagc ttttctgaaa aagcggtctt gagggatgta
     3301 agccatgtct cacagatagc ggtctttact ttacctgata gacggcaata aaaaagccca
     3361 gccgaacggt tcggctgggc tcgttaacga ggcgatgtga ttctatttgg ctgtgcgcac
     3421 ttggaactcg acgcgtcggt tgatcgcccg cgcccaagca gtgtcgtgtt cgatcagcgg
     3481 tttgctttcc ccggtactga cggctgacat gcggctctga tcgatcccca acgacgccaa
     3541 ttcattcatc acggtttgag cgcgctgcaa cccgagctgt tgattataac tatcgtcgcc
     3601 gcgattatcg gtgtggcctt cgacgacaat ggtcagattg ccacgctctc ttaaccgttg
     3661 ggcggcaaga tagacttgac ctttacccaa gtcggtcagg tacgcagagt cgaaattgaa
     3721 agcgaccggt gtgaaaacca aacgctccac ctcaactacc ttttcctgaa cttgaataac
     3781 agtcttttcg accgggactt cgacgatctt aacatgttcc ggcatcggaa ctggcggcgg
     3841 cgcgggtggt gccactcgca cggtgtgcac tggaaccttt ttggctacgc cgtaatcatg
     3901 caaggtttgt ggttcagcaa gtacggttgg gtgtggagta tggccaaaaa aagcctcttg
     3961 cgcccgagtg ccgctgacga gtaagtgaaa aggtcgaaag atagtccact cggccagcca
     4021 gccgactgga tacgcgagat aggcaagaac tcttaatgga tgcgattggc tatcgtcgta
     4081 ttcatcgggg gcagcaaaac tagcttgccc cgccaaacca attgccattg ctaaggtcac
     4141 agcttggacg gttcttcttt tcatgtttag tcctcctaaa aggctgacaa aacggcagtt
     4201 taagaggtag gaagttattt gtcaaacttt tgtttggcga ctgacttgta agcatcacaa
     4261 tcaacggttt aagacactcc aaagatttaa agtacaatag ttggcacttc acttgaggga
     4321 attggcatga atgtaaatgt tgataaaaaa gaccgtttaa taacgataac ctgggatcag
     4381 ccgcctttga acgtactcga cctatctttg ttgcgcgaac tcgatgcaat tctccttggc
     4441 tgcgcggccg agccagcggt agacgtcata gtgctgcgcg gccaaggatc caaagcattc
     4501 tccgcgggtg tggacattaa ggaccataca ccaaagaaag tgcccgaaat gctcgatgtg
     4561 gttcacggcg tcattcgtaa gcttttgagc gtgccccaaa tcactgtcgc ggcggtgcag
     4621 ggagcctgtc tcggcggcgg ctgcgaagtt gccagcgttt gcgatttcgt tgtcgccacg
     4681 gacgatagct tctttgctac gcctgaaatt ctcgtcggct gctatccgcc ggtggcgctg
     4741 gcgcggttcg cgacgctcat cggctaccac agggccgctg aaatgatcct caccggccgt
     4801 cgcttcactg cccatgaagc ccaagggatg ggattgatca atcgggtgtt tgccgatgct
     4861 gaactagaaa cgggcgtaga gagtttggtc ggcgaacttc tgagtaagag cggcgctgtc
     4921 atgcgtttga ccgtgaaagc gctgcgtgca ttatcgctgg gtgattttga cgcggcgctc
     4981 aggcgctcag agcaaattta ctgcgacgag cttcttagca ccgcggatgt cgaagaaggc
     5041 gtgcaagcat ttttgcagaa gcgtccgcca caatggcgcc atcgttaaag atcggcgctc
     5101 attcgcgcgt tccctggcta tgcagatttt caaataggcg gccatgtgat agtttcgcag
     5161 cgaatggctg aattcgaaat caaaattgcc actgacgcgc aggagatagt tgaagcgcaa
     5221 cgcttgcgct tccaagtgtt taatctagag ctgaccaagg gcctgcaaag ttcttatcag
     5281 agtggtttgg atgtcgacca gttcgatcct ttctgcaacc atctcatcgt ccgcgacacc
     5341 aaaagcaagg aagttgtcgg cacctatcga atgatgctcg gggcacaggc gcgccagcat
     5401 ttgggtttct attcggaaaa ggagttcgat ctaacgcgca taaaaaagtt ggatggcgag
     5461 ctcttggaat taggacggtc ctgcgcgcgc aaggattatc gcgaccgtgc cttgctgccg
     5521 ctcatgtggg aagcgatcgc cgactatgtg cgagcaaacc gggtgcgtta tctgttcggt
     5581 tgcggcagtt tgtatacgac cgatgtcgcc gagattagcg ccatctttgc gctactgaga
     5641 aacaagtatt acgcgcctga tagttgccgg gtcgagccgg tagccggatg ccgctttgag
     5701 ggtttaccgg atctgcaagt gtgcagtgcc gaggaagcgg cgctgtttca aaagctgccg
     5761 agtttgatta agggctattt gcgtattggc gctctggtct gcgggccgcc ggcactcgac
     5821 atcgagttcg gtactgcgga cttctttttg cttctcgact ttggcagctt gaaggaagcc
     5881 tttcttaaac gattgggcat gggcgacttg aggggcaatg aagcgcctgc ttaaacttgc
     5941 cgcgtttcta tctctctggg tattcttctt cgccctggtc gctctggttc acctgtggat
     6001 ttcggtgctc ggcatgccca atcgctggaa gattatcagc cggatcaatc gcacgtacac
     6061 gttgctattg cgatccattc tcaacatcaa attgactatt gtcgggaatg aggggcagct
     6121 cgaacgcggc ggttatgtaa ttattgccaa tcacgtcggt tacatcgatg ggatcgtact
     6181 tggtagtatt ttcccgatcg tcttcgtgtc aaaaaaagaa gtgcgcagtt ggcctatcgt
     6241 cggccagtgg aacattctgt gcgggacggt tttcatcgat aggcagcata aagagcaggt
     6301 cggcctgctg gtgcaggaga tgtcccggaa actgaagcac gaagccaata ttctgctctt
     6361 ccccgagggc acctcgacga acggcgaacg gatgttgcct tttcaaaccg tccctctggc
     6421 ggcgcctctg cgcagccggt cggtgatcgt gccgacgact ctcgcataca aatccctcga
     6481 cgacgtgcca attaacgcgg ttaatcgcga tttgatttat tggtatgggg acatggattt
     6541 cgtctcgcat ttttggaagc tgctagcgct gcgcagtatc gaagtgctgg tgacgataca
     6601 acccaaagtc gaatgctttc gctattcaga taattccgcg ggacgtaaaa aattggccga
     6661 ggactgttat aatcgcgtcc tcggccgaat aacggaaagt agtttggatc aggatgatga
     6721 gatagaagag gacggcgaac cgttagttcg cgcttaactg tttcttgatg ttttcgacga
     6781 attctttaag catggcgtca gccttcttgc ggatgatcgg ttggccgaat tcaccgatct
     6841 tgccgagaat gttcacgtcg gtctcaacaa gtagctcagt ccgattggcg tcaatctctt
     6901 tgaggttcat gattgtcttg ccgttaactg cgcctgccgc catgcggtct tttgcctcgg
     6961 cccgcaacgc cgcgcgccaa ttggtcttgt cttgttcttc gagaatgatc ttaccttgca
     7021 agttgagttt gatgggaccc acgcgcacag ttaaagcgcc ttcatacgtc gtctcgtcga
     7081 ttttgctcac cgactccacc ccaggtaatg atttggctac cttgggcaca tccatcaaaa
     7141 aatcccaaag cggttcgcgg gctacgggaa tctgggctgt ctgggaaaat ttcaacgttt
     7201 tttgcctttc ttgacgatct tcttttttgc cggtttgggc gccgctttgg ctttagcctg
     7261 ctggcgaatg tccgcgaggg ctttttccaa ggcgcgccgg acaaacactg ccgccatatc
     7321 ggtcttgtaa gctgcagaac ctctaaaatc gctgattggg tcgacagctt gtttggcttt
     7381 ttcaccagcc tcagcgaaag cttcggcctt tagcggttga ccgcgcagta ctgcttccgc
     7441 ttcggtagcg cggatcggtg tgacgccgac gctgccgagt gcgatacgca catcggcgat
     7501 agtcttcatc gacttatcaa gagtcaatac cgctgccgca gaaacggtcg cataatcatc
     7561 agccgtccgc ggcagaaatt taatataggc cgcgccggta tttggagcct gtttgggaac
     7621 cattacttcg gtgatgatct cgtcgggatt gagcacggtt tcataatagt ctttgaagaa
     7681 ctcatcgagc gaaatcgtcc gactgcccat cggcgatgtg gctttcattt tggcgccgag
     7741 cgcgattagg ctcggtggcg gatcttgatt cggatccgca tgggccaagc caccgccgac
     7801 ggtggccata ttccgcacgc gaagtgtcgc cacatggtga taggtttcgg caagcatggg
     7861 gatgcgtcgg cgtaccagcg acgagctttc taccgcccga tgggtggcca aaccgccgat
     7921 cgacaaaccg ccatctttta cctcgatgcc atttaagccg cgtactgact gcagactgac
     7981 taagcaggtg ggccggacca ggcgttgctt catcatgatc accaaagcag tgccgccggc
     8041 gatcaatttg gcgtcgctgc caaattcttt cagcgtcgca tgaacttctt taagattttt
     8101 cggggtgcga tattcaaaag gtatcattgc ggtgtccttg ctatttcgcg aattggtgcg
     8161 ctttctggat cgattcgatg attttataat agccagtgca acggcacaga ttgcccatca
     8221 tccaatcctt gatttcgtct tcgttgggtt tggggttttc tttcaacagc gcggtggcgg
     8281 caaccacttg gccaggggtg caaatgccgc attgaaagcc gccatattgg ataaagctct
     8341 tttggaccgg attgagttcg ccgtttttcg cgaggccttc caccgtagta agatcgtggc
     8401 cgtccgccat gaccgcaagg gttaggcatg agctaatcat gtcgccgtcc atcagcaccg
     8461 tgcaggcacc gcagacgccg acgctgcaac cttccttggt gccggtgagt ccgagatcgt
     8521 aacgcaaaca gtcgatcaac atgcgattag ttgggacatc gacctcgtgt cgtttgccat
     8581 tcacgcggaa tttgatcttc ttagtcatcg ctgttccttt cgcagaatgt atcgcgcgga
     8641 gttttcagcc aggccggaaa aaccactagg gccttcttat agtgagaaat tggtgaaact
     8701 gcaagccatt ttgccggcaa attccgcgcg aaatgatagc tgaagtgcag caaacggtgc
     8761 gcgctcgatt actatcgctg aggcgcttta tgccgatggt ttttgccgat cggaaccctc
     8821 taactcgcta aaatttgatc actgaacgga tgctctggcc ttcatgcatt agatcgaaag
     8881 cctcgttgat ccggtccaaa ggcaaaacat gggtaatcat gggatcgact ttgatctcgc
     8941 cgcgcaaata acgctctacg tagccaggta attgcgagcg gcctttgacg ccgccaaaag
     9001 cggtaccgcg ccagacgcga ccggtcacca gctgaaaagg ccgcgtggcg atttcttggc
     9061 ccgctgcggc gacgccgatg atggtcgact cgccccaacc tttgtggcaa cactccagtg
     9121 ccgcgcgcat ggtgtttaca ttgccaatac actcgaaaga ataatccacg ccgccatcgg
     9181 ttaagtcgac gattgcttgt tgtatcgtta tgccgtcggc ttgcggcacg atgaaatcgg
     9241 tggcacccaa ctgcttcgcc atctcgaact ttttctcgtt caaatcgaca gcgatgatgc
     9301 gttccgcctt agccatgact aggccttgaa tcacactcaa accaataccg ccgaggccaa
     9361 agacggcggc ggatgagccc ggccgtactt tcgcggtatt caacactgcg cctatgccgg
     9421 tcgtcacgcc gcagccgagc aaacaaacct tgtcgagcgg cgctgccgga ttgattttcg
     9481 ccacggccat ttccggcagg accgtgtatt cggaaaatgt cgaggtaccc atgtagtgca
     9541 agatttcttt gccgccgagc gagaagcgcg atgttccgtc gggcatgacg cctttgcctt
     9601 ggctgagccg gatggcgccg caaaggttcg tccgcccgga cttgcaaaac ttgcactcgc
     9661 cgcactcggg aatatacagc gggatcacgt ggtcgccggg tttgacgcta gccacgttgg
     9721 caccgacttc catcaccgtg gcgccgccct catggcccat gatcgccggg aacaatcctt
     9781 cgggatcttt gcccgataga gtatacgcat cggtatggca gacgccgctc gcgacaattt
     9841 ttaacaagac ctcgcctgtt tttggcgcgc ccacttcgac ttcttcgatg gttaaaggtt
     9901 taccggattc ccaggcaacg gctgcgcgcg atttcataga cttcctccgt gacgcgggta
     9961 tgcgatcgct gcggaacgaa aatttcataa cgtaaaatac aatcggaaac tagtggccca
    10021 ttcgattgct ttcgcacaag ctgccaatta ccgaaacagc gtgtgtgcag cttgcgcatg
    10081 cgtggagctg gtgtgacggc ggcaggaggc gcgggaaccg ttttctttcg acctagccca
    10141 agcgacaacg gcgtttagtt cgttccttcg ccggcgccgc gtatgggaag cgcaatatct
    10201 catttcaaac gtcgcatggc agagaaaaaa tctcgggacg atccgccaaa tttgcgctgg
    10261 gaagacttct ggcggtaata acgacaggct ctcagacgcg gcagatcgtt ttgtcgcgtt
    10321 cgcccgccgg cggaggttct gaatggctta tatggtcctt ctgccaatca gagtagtgtt
    10381 tgctggtttc tcggaatcga ggcggattca ctctcagaag cgatatacga ctcttggacc
    10441 ggcgttgaaa taggtaaaac ccgaagctga tcccgccgcg aaattaccgc cctcgacgtt
    10501 gatttccagt gaaagattct ccagtggttt gattccgatg ctcccgctgc cgccatgaat
    10561 gaagttattg gacgcaatcc cgttgcggcg aaaagtctca tacccgagat agccctcgac
    10621 gtaggcgtaa taagtccgat tatcgaaata catagacgtg aatccgcgat tggctatgta
    10681 gttgttggga tcgaagtagc cgctgccggt ctgcttttga aagtcgagca agcgaaaacg
    10741 atgaccgata acgacgcgtg gcttcagata taccgagtat tgcgtcgcca attgcaactc
    10801 gttggcgtga ttgccgtccg ataaactttt gtagttatag cggccctgga cggaaagccg
    10861 ttcggtcaaa ttttgttgta ggttgaagct accaaccgtc atacggatgc gattttgcac
    10921 cagttccgcg gtgtcgctca atacttctcg cgtcacgcct gtgccgatgc tgccgtcaaa
    10981 cagttttgcg tctatccgca gctgtccggt cggaaacgtc gaggtgtgac ggtcggcaac
    11041 ctggctgaag ccaatggcta cgccggcgct taccacatcg ctgagtcggc tgtaaagttt
    11101 ggcagaaaaa tcttcggcgc gattggcgcg ggtgggatcg ctcgcctccg tatggcggta
    11161 gtccataccg agtttctgat tgccgaccca gtaagtcgcc gcgaggttgt aacggtcgac
    11221 gcggttctga tcggaatcgg aatagtagtt gtagcggccg tcgagagtcg gccggatttc
    11281 gtcgtcaatt tccaagttca atcgggccag ttcacgttgc tgcttgggag ttccgggttt
    11341 caaagtatcg cggctctcta gggctgcaac gcgattgcgc accgctaatt gcgcccgcgc
    11401 caggccagcg cgcgcgtcga aatcgtcgtc acgagcgaca atctgctgat agattgggat
    11461 ggattcagca ttgcgtcctt gccattgcaa cgcgtcggct ttgactaaaa gcgcgtcttt
    11521 gtgctgcggt gactgcgcca aaactcgatc cgcctcgatg atcgctgccg caagctcgcc
    11581 ggaccaagcg agcacccgcg ctaggtgagt tcgtgcgggg atgttttgcg gctccaacgc
    11641 cacaacattt gcgagctcag cacgtgcctc cgccaaatgg ttagtccagg aaagatagat
    11701 cgccatctgg actcgttccg cggctgagaa acctgcggca tcgaggttga gcgccctaat
    11761 gtagggctcg gcggcatcgt cgatcctatc ttggcggacc aattgatcgc cgagttgttt
    11821 atagagtttc gcttggccgc gcgggtcagg cgttgattga atcgtccggc ggagctccga
    11881 ttcgtcttgc gacactgcta tcgaggtgta gccggaaatt agagtcagct gaaaaaacag
    11941 cgcaaacaaa ttaactttga gagtgcggcg tttcgcgggg atcagattca cgatgtcggc
    12001 gccattccta ccatcgtttt tttgtgccga gcagctcttg ggcatagccg atgacacaga
    12061 ttggggacat gatcgcttga tagatcaaca tgtagacaaa gaagccggcg aaattttgcc
    12121 gtactctaag ctctaaaaca tcgaataccg ctttttgctt tttgtacatc gccaacacta
    12181 tcagaaaagc tagcggcatt accagcacag tcatgggacc cacaatgaag cgataaccca
    12241 aaagagccag aacaacgccg ggaagaaata cgaaagcata gaaaagatcg ataaacggga
    12301 taataaaatc tatcgccaca aaaaacgctg caagccggga agtactccag accagctgcc
    12361 cgtgttgctt taaaccttcg atcataccgc gcgcccagcg ttttcgctga cgatagaagc
    12421 cgatgaagct tgttggtgcc gaggtgaaac cgatggcggt gggctcgaaa ccaatgcgcc
    12481 aaccactttt gatgagcgcc catgtcataa caatgtcctc gccgattacc gacggccagc
    12541 ctttgtgggc cttgacgact ttgctgtgga acgcactgaa cgctccctga gctaccagtg
    12601 tgccctgata gagcgactgc tgtcgttttg ccgaggcaat gccggtgaag tagtcccatt
    12661 cctgcaggcg agtcatgaaa gtggctcgag aatttttcac caacacacag ccggcgaccg
    12721 cggcggtgtc ttgcggatcg gttaaaaagc gcgtgacaat gcgtcggagt gcctggggat
    12781 gtaggaatgt atcggcatcg atacagacga ggatgtcatg tgctacatac tccagtcctt
    12841 ggttgagcgc catcgccttg ccgccatgat tgccccgaac gattttgacg ttggccaact
    12901 ccagagcttc gatcactcct accgtattgt cgctcgagcc gtcgtcgacg acgatgatct
    12961 cgacagtccc cgaataatct tgggcggcga tcccgcgaaa agtctccgct atgttttctt
    13021 cttcgttata agccgcgatg agtaagctga tcggcggata ttcgagcagc cgcggcaagt
    13081 cgggcggccg atcgaggacg acgctggaaa gaatatgcgc gttaagaaac ccgggcagca
    13141 aggcaatgaa gaaaataatc ccaacggctg tgaaatagcc ggtaatcggg gagagttcgc
    13201 caatccagga ttgggcgaga taccaggaaa ccgtgaccca taacatcgct aacaacgtcg
    13261 aaactataaa tttaccctta accggaaggt aataaggacg cttactgcgt tgccattggg
    13321 gccacgttaa attggatgtt ggttcagacc atcgcgcgac gatacttggc tcccgccatg
    13381 acgtttcatc gctgggagtc acagtgtcgg attgctttcg cttcaacgag ccgttggccc
    13441 gaagactatc gttttggtct tgcatatggg gtttaccgcg cttttttcta gagaccgaac
    13501 attcgacaca acgcaacgcc gataccaacg gagattgacc gagattcggt cgttttttgg
    13561 agttttcgac cctagctttt cgtcttcgaa cgaagtcttc ggaacaattt cgacaaagag
    13621 cagtgccttg accaacattt tggccgtatt gcgaggtacg accgactttc aggccgcgat
    13681 ggttcattgc aggagccagt gcggtaagtt ttcccgctga tccgatccgg gataatcaca
    13741 ttttgccggg gcaattaagg ttgcattacc gcatggaatc gtggaaagga tagaaaaaat
    13801 ctaaaggggg acgcttatgt ttcttgagat gagaacctac gtattgaaac cgggcatgac
    13861 caataatttt gtcgaaggct ttgccgcggg tttgccggcg cggttgcaaa tttccaaatt
    13921 gctaggttta ttttgctccg aggtcggtgg attgaaccgg gtaactcatg tctggcctta
    13981 cgaaagcttt gaggaccgtg agcgcatcgg taatgaagcg cgtaagactg ggaagtggcc
    14041 gcccaagact caagagttta tcgttaccca ggaaaacaaa attattcagt tggcgccgtt
    14101 ctcgccgccg ttgccggaaa agaagcttgg cgagatttat gagtttcgca cttacaccta
    14161 tcagcccggc accatgcccc tcgttatgga gcgtttcggc aaagtcattc cagaacggac
    14221 caaaatctcc ccgttggtgt tcgccggcca gactatgatt ggtcctttga accagttcat
    14281 tcatgtgtgg gcttacaaag atgctggcga acgcgagcgg ctgcgcggcg aggcggcgaa
    14341 gaaagttgcc ggttggccgc cggcgacccg ggaatttatg gtcgcacagg agaatacgat
    14401 catgactccc gccgcttgcg cgccgttgaa ttgacctcag cttcaacagc actgcggcga
    14461 tccgcgcgtt gtgtggcccg gaatgcgatg cgagtgcaaa aggggagcgt tcacgctccc
    14521 ctttttttgc ctaattcttt gtcgctgtgc cggctgaatg gctggaagtg atagtgtttt
    14581 ttagatacat cgttgccatg tctatgtttt acaaatcgct tattcgacca gttttatttc
    14641 gcaaagatcc ggaagaaagc catgacgccg ttttgcgcat gttggcgcgc aacgagtggc
    14701 tttacggcac gcttgaggaa ttttacaaaa tcgaagatga acgactcgag gttaaaatcg
    14761 gcccactcgc ttttgccaat ccggtggggc tcgccggcgg tttcgacaag aacgcgttag
    14821 cgccaaaaat gatttcggcc tttggcttcg gatttatgga agttggagcg attactgcgc
    14881 aagcacagcc cggcaaccct aaacctcggt tataccggct gccggaagac gccgctttga
    14941 tcaatcgact gggcttcaat aatgagggcg ccgaagcgat cgcgatcagg ctcgattgct
    15001 tgcgcgctcg gggcgggcgg ccgaagattc ccctgggtat gaacatcggc cgtaccaaga
    15061 tcgtcgagac taaagatgcg gtggcggatt ttctttcttg cttcgacgcg ctgtttccgc
    15121 acggtgattt cttcacggtt aacgtgagtt cgccgaacac gccgaacttg cgcgagctgc
    15181 aggataaaaa tttgttgagt gagctgcttt cggccctcca ggaaaaaaat acgcaactcg
    15241 ccaagcaggc aaagatcgat ccgaagccgg tattcgtaaa aatcgctccc gatatggaat
    15301 tcgatcaagt ggacgaaatt atcgaagtcg ttgaccagga gaagctgacg gggatcgtcg
    15361 ctaccaatgc gaccgcgttc aaacgcgaag gcctgaaatc ggccaacggt cgtgagcctg
    15421 gcggattaag cggccggccg attacggcga tggtgacgca cttcatttct cacatctatc
    15481 gtaacactcg cggcagactg ccggttatcg gagtaggcgg tattttcaac gccgaagatg
    15541 cttacgagaa gatcaag
//
LOCUS       DATLCH010000363        10055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7434440, whole genome shotgun sequence.
ACCESSION   DATLCH010000363 DATLCH010000000
VERSION     DATLCH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7434440"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VMZ02_10830"
     CDS             complement(<1..218)
                     /locus_tag="VMZ02_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72474.1"
                     /translation="MASFIEMSDAIAAILKALWVSASSRNIVARILVNALVGRIVFRY
                     QDTIFDVNDHLFGLRHWIGGEGELPYEVR"
     gene            complement(872..960)
                     /locus_tag="VMZ02_10835"
     tRNA            complement(872..960)
                     /locus_tag="VMZ02_10835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(924..926),aa:Leu,seq:taa)
     gene            complement(1059..3188)
                     /locus_tag="VMZ02_10840"
     CDS             complement(1059..3188)
                     /locus_tag="VMZ02_10840"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HUR72475.1"
                     /translation="MIPFVIVISCLSLAFAVFLARQVLQKDTGTQKMQDISNAIKEGA
                     EAFMSRQNRTIISLSVALAVLIFLLYGFVRTPSTADPVPPLELAFWTTLAFVLGAACS
                     VIAGYVGMWVSIRANIRTAAAVRSSLNEGLQIAMRGGAVSGLFVVAMSLLGVGGLYAL
                     LEALGHDPQKIPFTIVGYGFGASFVALFAQLGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVGLYPYFGLKGILFPLVA
                     RALGLLASIVGIMVVKTKEDGDAMAALNRGYYVTSALAIVGFFFTAKSLLAVDVSKYP
                     EAESAWFYFFLCGVVGIVMAQAFVYITQYYTEYKYRPVREIAEASKTGPATNIIAGIA
                     VGLECTAIPVLVISVAIITSYYLGLNSGVKHAGLFGTAVATMGMLGTAAYILAMDTFG
                     PITDNAGGIVEMSGQPEEVRHKTDRLDAVGNTTKALTKGYAIGSAALAAFLLFSAYLD
                     EVAHYGHPLEVVNIAKPEVFVGGLLGAMLVFLFSSFAIRAVGKAAYYVINEVRRQFRE
                     NPGILLGTVKPDYRRCVDIVTIGALKQMVVPGLLAVTMPVAVGLVFRIFGIGAEAVAA
                     FLMVGTITGILMATFFNNSGGAWDNAKKYIESGQYEGGGKGSDPHKAAVVGDTLGDPF
                     KDTAGPSLHVLIKLLATITLVLAPLFV"
     gene            complement(3220..3930)
                     /gene="tatC"
                     /locus_tag="VMZ02_10845"
     CDS             complement(3220..3930)
                     /gene="tatC"
                     /locus_tag="VMZ02_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HUR72476.1"
                     /translation="MTLTEHLEELRWCLLRSVIAVLVGSTVCYYFSNAIFAFMVAPLR
                     ENLQPGQGLIGTSVTEAFFSEIKVAIAAGVLFTCPYIFYQIWRFIAPGLSGGEKKLVL
                     PFVTCATLFFLGGAYFCYRVVLPVAFKYFVEQYNTMGVLPAIRIGEYFTFFFRMVLAF
                     GITFELPVFTFFLVRLGLWNYRLMISSFRYAIIGIFILAAILTPTPDVINQSLLALPM
                     LVLYIASIGVAYIWRRTD"
     gene            4562..4834
                     /gene="rpsT"
                     /locus_tag="VMZ02_10850"
     CDS             4562..4834
                     /gene="rpsT"
                     /locus_tag="VMZ02_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HUR72477.1"
                     /translation="MADHPSVEKRYRQSVKRRTRNIEIKSRLRTLMKKARQAIESNNH
                     DVASSQLQGVNKALGKAVSKGIVKKNTASRWLSRLARSAHRSKSAS"
     gene            complement(4924..5538)
                     /locus_tag="VMZ02_10855"
     CDS             complement(4924..5538)
                     /locus_tag="VMZ02_10855"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HUR72478.1"
                     /translation="MLDSAVWPRPSLPFFAAVGILIALSGCGYQWVGQSSLLPKDAQT
                     VYVEPFINRTRDIGLEKELTSALRSEFYRRGQLRVVDAPEQADIILSGVVRSLETTVA
                     SVNRYDEVLQYEAQLVLDATLRRREPNEILWRGQAIRLNELYGGSRAAVVTTSSEFST
                     GTLSANNVRNMTDVQLTESERRGVRDRLMERFAKELHQRVIEMF"
     gene            complement(5542..8136)
                     /gene="leuS"
                     /locus_tag="VMZ02_10860"
     CDS             complement(5542..8136)
                     /gene="leuS"
                     /locus_tag="VMZ02_10860"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HUR72479.1"
                     /translation="MNERYQAQSIEDKWQKLWADEQAFRAIEDPGKPKYYVLEMFPYP
                     SGRIHMGHVRNYSIGDVVARYKRMRGFNVLHPMGWDAFGLPAENAAIERKVHPEIWTQ
                     ENILYMKTQLKRMGLSYDWEREIATCQPEYYRWNQWIFLQFYKKGLAYKKSSFVNWCP
                     SCETVLANEQVIDGACWRCDSTVVQKELEQWFFRITDYADELLDDLDQLSGWPEKVLT
                     MQRNWIGKSIGAEIDFPLVDADGALKIFTTRPDTIFGATFISLAVEHPLAMPLSAGTA
                     QEKAVKLFIERFRKISQAQRAAAEEEKEGVFTGAYCRNPFTGENIPIYLANFVLMEYG
                     TGAVMAVPAHDQRDFEFAKKYSLPIRVVIHPKDKQLSADVLSEAYVDEGVMRDSGEFS
                     GTANVAGKEKIAVFAEANGFGKKAIRFRLRDWGVSRQRYWGTPIPIVYCNTCGVVPVP
                     EDHLPVELPKDVPFSGKGGSPLQESAAFNRVECPKCGGAARRETDTMDTFVDSSWYYL
                     RYTSPDFARAPVDVSRGRYWMAVDQYIGGVEHAVLHLLYARFFTKALRDIGLTHVDEP
                     FTNLLTQGMVSKETYRCVEHGWLFPNELAGAEKGGGLCPHCGRTVDKGRVEKMSKSKK
                     NIIDPEDLITAYGADTARLFTLFAAPPEKDLEWSDQGVEGAYRFLNRLWRFVYQHRAL
                     GSAKSETTADEVDLELRDLRRAIHRTIKKVSDDIDGRFHFNTAIAAIMELFNSLSAVA
                     QDPLKRAAATTIISEGLDAIILLLAPFVPHVASELWQQLGHHERLDRVAWPSYSEAAL
                     EEEQLLIVVQVNGKVRGKMTVPAEITQEQIESGALADAKVMSFCEGKKIKRLVYVPRR
                     LINIVVEG"
     gene            complement(8146..8601)
                     /gene="nusB"
                     /locus_tag="VMZ02_10865"
     CDS             complement(8146..8601)
                     /gene="nusB"
                     /locus_tag="VMZ02_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HUR72480.1"
                     /translation="MPQDSSDNTPAAPRTMRRKGRELALQALYQIEITGDSSMAAVDL
                     FLQHFEGGAKAKEFARRLVSGVVSQRPELDRLIQQSTEHWKMERLAKVDHEILRLAAY
                     EVAFCPDIPDAVSLDEAVEIAKRFGSDDSAAFINGVLDQIAQTSRARSE"
     gene            complement(8641..9108)
                     /gene="ribH"
                     /locus_tag="VMZ02_10870"
     CDS             complement(8641..9108)
                     /gene="ribH"
                     /locus_tag="VMZ02_10870"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HUR72481.1"
                     /translation="MARILEGKPDAQGLKIGIIVSRFNGFVTERLLEGALDGFSSHGG
                     AENDLTVVRVPGAFEIPIVADKMATSGKYDALVCLGAVIRGDTPHFDYVCDAVTQGLG
                     DAVKTHKIPVGFGVLTTDNVQQAMDRAGSKDANKGYEALLVAVEMVNVLRQVS"
     gene            complement(9137..>10055)
                     /gene="ribA"
                     /locus_tag="VMZ02_10875"
     CDS             complement(9137..>10055)
                     /gene="ribA"
                     /locus_tag="VMZ02_10875"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HUR72482.1"
                     /translation="RAHTIRSVIADATKHEDLMVPGHVQPLQARSGGVLARSGRTDAS
                     VDLSRLAGFKPAGVICQILDEDGSVAALPTLERLAEKHGLKILSVASLIAYRLRNESL
                     VLRVAEEEFPTLHGGKYKAIVYRNVVDGAEHMALVKGDITKAAPVLVRLHSECLTGDV
                     FGSERCDCGDQIRESLRRIDELGCGILVYMHQEGRGIGLTNKIRAYALQDQGRDTVEA
                     NLELGFKEDLRDYGIGAQILRDLHVENVSLLTNNPRKISGLENYGVKVVERVALEAQP
                     LKTNIHYLRTKQRKLGHMLSNLEDRTAKR"
BASE COUNT         2434 a         2873 c         2617 g         2131 t
ORIGIN      
        1 cgaacttcat aaggcaattc cccttctcca ccaatccaat gacgcaatcc aaataaatga
       61 tcgttgacat caaatatagt atcttgatat cgaaatacta tgcggccaac caaagcattt
      121 acgagaatgc gagcaactat attgcggctc gaagccgaaa cccacagagc tttaaggatt
      181 gctgcgattg cgtcggacat ctctatgaat gacgccatca atcaggccgt gaatctctgg
      241 cttaaagaac atcgaaaaag aaagaggtcg aaaaaatgaa gcgcaaaaag gtgaaggatt
      301 ggttttccaa cggggcgcag tttactggat aaaaaactat cgtaacggca aagcctaccg
      361 tgatcggcgg atcggacaaa gaaagcgacg caagaaagct gttgcggaag cgccatgcga
      421 aatggcccta agtcgattca cgggtcggct gccggtttga attcgagggc tggccgttgg
      481 ctatgtcacg gaccacctcg tcgagggtaa ggcgacggat tcaaccttgt tagcctattt
      541 cggggaattc aaaccacatg acgtgggcac ggacttgatc aatcggtatg tccctgcgcc
      601 tagagcaaag cgcggcaagc gctacgatca acaggaagct atcggcaatc aagcgaatgt
      661 tgaaccgggt acgatttggt cagcgatgac gatctaaggg aagcagtgcg caaggcgtgg
      721 gaacatgcgc agaaccagga agcggtggca aagcttgtcg cggtaagatc gaatcggtaa
      781 tccgttaggc agggtttggg cacaatgagc ggacacaaca gcttatgaac cttcggagcg
      841 gcggacccat ggtaggtatt ttaattagtt atggtgcccg gagccggact cgaaccggca
      901 cagcgctttc gcaccgaggg attttaagtc ccttgtgtct acctattcca ccatccgggc
      961 tcacttcagt tgagcaagtg aacttctagc acaatttaat cggccacaaa atgcgaaggc
     1021 ctctcgcggg agaggccttc ttccattcga ctgctttttt agacgaatag cggcgccagg
     1081 actagagtga ttgtcgccaa cagcttaatt aggacgtgca agctcggacc tgcggtgtct
     1141 ttgaacggat cgcccaaagt atcgccgaca acagcggcct tatgcgggtc ggaacccttg
     1201 ccgccaccct cgtactgacc gctttcaata tactttttag cgttatccca cgctccgccg
     1261 ctgttattaa aaaacgtcgc catcaagatg cccgtgatgg taccgaccat caagaaagca
     1321 gcgacagcct cggcaccgat gccgaagata cggaaaacca gtccgactgc cactggcatg
     1381 gtcaccgcga gtagtccagg aacaaccatc tgcttcaacg cgccgatggt gacgatgtcg
     1441 acgcagcgac ggtagtcggg ctttaccgtt cccaggagga tgccgggatt ttctcggaac
     1501 tgccggcgca cctcattgat aacgtaatag gcggctttgc cgacggccct gatagcgaag
     1561 gaagaaaaca gaaaaaccaa catggcgccg agcaagccgc cgacgaatac ttccggcttg
     1621 gcgatgttga cgacttccaa cgggtgaccg taatgggcca cttcatcgag gtaagcagag
     1681 aatagtaaaa aagccgccag cgccgccgat ccgatggcgt aacctttggt caaagccttg
     1741 gtcgtgttac ccaccgcgtc gagccgatcg gttttgtggc gcacttcttc gggttggccg
     1801 ctcatctcaa cgatgccgcc ggcgttatcg gttatcggac cgaaggtgtc catagccaga
     1861 atataagccg cggtgcccaa catacccatg gtcgccaccg cggtgccaaa taggccggcg
     1921 tgcttcacgc cgctgttcag tccaagatag taggaggtga taatcgccac cgagatcacc
     1981 agtaccggaa tagcggtgca ttccagaccc accgcgatac cggcgatgat gttcgtcgca
     2041 ggtccggtct tggacgcctc ggcgatttcg cgaaccggcc gatatttata ttcagtataa
     2101 tactgcgtga tatagacgaa tgcctgggcc atcacgatgc cgacgactcc gcagaggaaa
     2161 aaataaaacc acgccgactc cgcttccggg tacttcgaca catcgaccgc cagcaaagac
     2221 ttggcggtaa aaaagaaccc gacgatcgca agcgcgctgg taacatagta gcctctattg
     2281 agcgccgcca tggcatcgcc gtcttcttta gtcttcacca ccatgatacc gacaatcgac
     2341 gcgagcaacc ctagcgcacg ggcgaccagc gggaaaagaa tccctttcaa gccgaagtat
     2401 ggatacaggc caactccgag aatcatcgcg ccgatgtttt ccgctgcggt ggattcaaac
     2461 agatcggcac cgcggccggc acaatcgccg acattatctc caaccaaatc ggcaataacc
     2521 gccgggttgc gcggatcgtc ttcgggaatt ccggcttcga ctttgccgac cagatcggcg
     2581 ccgacatcag cggccttggt gtagataccg ccgcccaatt gagcgaataa ggcgacgaac
     2641 gatgcaccga aaccgtaacc gacgatagtg aacggaattt tttgcggatc atgaccgaga
     2701 gcttcgagca aagcgtaaag cccgccaacg ccgagtaagc tcatggcgac cacgaacaga
     2761 ccagacaccg cgccgccacg catggcgatc tgcagaccct cgttcaaact cgagcgaacc
     2821 gccgccgccg tgcgaatgtt ggctcggatg gagacccaca taccgacata tccggcgatc
     2881 acggaacagg ccgccccgag aacgaacgca agcgtggtcc aaaaagccaa ttccaacggt
     2941 ggcaccggat cggccgtgct cggcgtgcgc acgaacccat agaggaggaa aatcagcacc
     3001 gccaaggcca ccgacaaact gataatcgtg cgattctgcc gcgacatgaa cgcttctgcg
     3061 ccttccttaa tcgcgttcga gatgtcctgc attttttgag tgccggtgtc tttctgcaag
     3121 acctggcgtg ccaggaacac ggcaaacgcc agtgacaggc agctaataac aataacaaat
     3181 ggaatcatga ctcctcctca tccctagtaa caaacttttc taatccgttc tgcgccaaat
     3241 ataggcgacg ccgatactcg cgatgtacaa tactaacatc ggcagcgcca agagactctg
     3301 gttgatgaca tcgggggtcg gcgtcagaat cgccgccaaa atgaatatac cgatgatggc
     3361 gtagcgaaac gagctgatca tcaatcggta attccacagt cccagccgca caaggaaaaa
     3421 cgtgaagacc gggagttcga aagtaatccc gaaggccaat accattctga aaaaaaaagt
     3481 aaaatactcg ccgattcgaa ttgccggcaa tactcccatg gtgttgtatt gctcgacaaa
     3541 atatttaaaa gccacaggaa gaacgacccg atagcaaaag tacgcaccac cgagaaaaaa
     3601 caaggttgcg cacgtgacga acggcagtac gagtttcttt tcgccaccgg acaagcctgg
     3661 cgcgataaat cgccagatct gataaaaaat ataaggacag gtgaacaata cacccgccgc
     3721 gatagcgacc ttgatctcgc tgaaaaaggc ttcggtaacg ctggttccga ttaaaccctg
     3781 tcccggctgc agattttctc ggagcggcgc aaccataaag gcaaagatcg cgttcgaaaa
     3841 gtaatagcaa acagtcgaac cgaccaacac tgcaattacc gacctaagca aacaccaacg
     3901 aagctcctcg aggtgctccg tcagagtcat gttagcaatg gtcgtcactc ttcagaataa
     3961 cgccgaaatt gcaaaaaagg ggaaaggtta tccccttccc cttttttggt tgtctgagct
     4021 aagaccaaaa tcacttactt ggttttagcc tcgtctttct tttctgctgg cttgtccatg
     4081 cccgctgccg gagcgctatc cttcgctggt tcagcgggtg gagccgctgg ttccacaggc
     4141 gctgccggag caggagccgg cggcggcgga ggcggagctg gttcctcttt cttgccgcaa
     4201 cccacaaccg tcaaagtaag agcaaacagc gcaatcgccg acaatgaaac aaatttgttc
     4261 cgcatatctt tgttcacctc ctttcccgtc acacatccct cgattcactt gcgttagtcg
     4321 atttttatta cataagtgtg gcggagattc aagcaatcta cccacatatc gctaccgaag
     4381 tatagccccg ctccataatg aaaaaccaga ttgcgcggcg attttcttag ggcaaccgcg
     4441 gcgagtcatt gtctcgccgt ggatgaacaa cacatgggcg accttggggc tattccttca
     4501 cattgactct cactactccc gatgctacaa aagatcacgt aattttttct ggaggcaatc
     4561 cttggcagat catccatcag ttgaaaaacg ctaccggcaa agcgtgaaac gacgcacgcg
     4621 aaacatcgag atcaaatcga gattgcgcac gctgatgaaa aaagcccggc aagcgatcga
     4681 gtccaacaac cacgacgtcg cgagcagtca acttcaaggt gttaacaaag ctttgggcaa
     4741 agccgtcagc aagggcatcg taaagaaaaa caccgcatca cgttggctat cccgtctggc
     4801 tcggtccgcc caccgcagca aatcggcgag ttaaactaac tcgctccaaa tacgaatgat
     4861 ctttcggcgg cgacgcaagt tgccgccgat cttatcttag ctgtactcta atccattttc
     4921 tgtttagaac atttcgataa cgcgctgatg aagttctttg gcgaatcgtt ccatcagccg
     4981 gtcgcggaca ccccgtcgct cactttcggt caactgaacg tccgtcatat tgcgaacatt
     5041 gttggcgctg agtgtgccgg tgctgaattc cgacgacgtg gtcacgactg ccgcgcgaga
     5101 tccgccgtac aactcgttta ggcggatcgc ttgcccgcgc cagagaattt cgttgggttc
     5161 acgccgacgt aatgtggcgt caagcaccaa ttgcgcttcg tattgcagca cctcatcgta
     5221 tcggttcacc gacgccacag tagtctcgag agaacgaacc accccactga gaatgatgtc
     5281 ggcctgctcc ggagcatcga ccacgcgcag ctggccccgg cgataaaact cactgcgcag
     5341 tgccgaggtc agctcctttt caagaccgat gtcgcgggtg cgattgatga acggttcgac
     5401 gtatacggtc tgggcatctt tcggcagcag cgacgattgg ccaacccact gatagccgca
     5461 accgctgagc gcgattaaaa tgcctaccgc cgcgaaaaac ggcaaactag gccttggcca
     5521 aaccgcagaa tctaacatgg ttcatccctc caccacgatg ttgatcaaac ggcgcggtac
     5581 gtagaccagc cgcttaattt tcttgccctc gcagaagctc atcactttag catcggccag
     5641 ggctccggat tcgatttgct cttgggtaat ttcagcgggg acggtcatct tgccccgaac
     5701 tttgccattg acttgtacga cgatcagcag ttgttcttct tccagcgcag cttccgagta
     5761 gctcggccat gcgacacgat cgagccgttc gtggtggccc aattgttgcc aaagctcgct
     5821 cgcgacatgc ggcacaaacg gcgccagtag taggataatt gcgtcaagcc cctcgctaat
     5881 gatggtagta gcggccgccc ttttcagcgg atcttgggcg acggcgctga gcgaattaaa
     5941 cagctccatg atcgcggcaa tggcggtatt gaaatgaaat cgaccatcga tgtcgtcgga
     6001 aacttttttg atggttcgat gtatcgcccg gcgcaaatcg cgcagttcca agtcaacttc
     6061 atccgctgtc gtttcactct tcgccgaccc tagtgcacgg tgctgataca cgaagcgcca
     6121 caggcgattg agaaatcggt acgcgccttc gacgccttgg tcgctccact cgagatcttt
     6181 ctcgggcggc gcggcaaaca aggtgaacag tcgcgcggta tcggcgccat aggccgtgat
     6241 caagtcttcc ggatcgatga tgttcttttt cgacttggac atcttctcga cccggccttt
     6301 gtcgacggtt cgaccgcaat gcgggcacag gccaccgcct ttttccgcgc cagccaattc
     6361 attgggaaaa agccagccat gctccacgca cctataggtt tccttggaaa ccataccctg
     6421 ggtcagcaaa ttagtgaacg gttcatcgac atgagtcaga ccgatgtcgc gcagggcttt
     6481 ggtaaaaaag cgcgcgtaaa gtagatgcaa tacggcatgc tcgacaccgc cgatatactg
     6541 atccaccgcc atccagtagc gtccccggct gacgtcgacc ggcgcgcgtg caaaatcggg
     6601 cgacgtatag cgaagatagt accaggaaga atcgacgaag gtatccatgg tatcggtctc
     6661 gcgccgcgcc gcgccgccgc actttggaca ttccacccgg ttgaacgccg cgctttcctg
     6721 caagggcgac ccgcctttgc cggaaaacgg tacgtccttc ggtagctcca ccggcaagtg
     6781 atcttcaggt accggcacga ctccgcaggt gttgcagtaa actatcggta tcggcgtacc
     6841 ccaatagcgt tgccgtgaca cgccccaatc gcgcaagcga aaacgaatcg ctttcttgcc
     6901 gaaaccattg gcctcagcga agacggcgat cttttccttg ccagcgacat tggccgtgcc
     6961 ggaaaactca ccactgtcgc gcatcacgcc ttcatcgacg tacgcctcgc tcaacacgtc
     7021 agcactgagc tgtttgtctt tgggatgaat gacaacgcga attggcagag aatatttttt
     7081 cgcgaattca aaatcgcgtt ggtcatgggc tggaacggcc atgaccgcgc cagtgccgta
     7141 ctccattaga acgaagttgg ccaaataaat cggaatgttt tccccggtaa aaggatttcg
     7201 gcaataggcg ccggtaaaaa ccccttcttt ttcttcctcg gcagcagcgc gctgagcctg
     7261 gctgattttc ctgaatcgct cgatgaacag cttgacggcc ttctcctgcg ctgtacccgc
     7321 cgacaagggc attgccaacg gatgttccac cgccagcgag atgaacgtgg cgccaaagat
     7381 cgtatccgga cgagtcgtaa atattttgag cgcgccgtca gcgtcgacga gcgggaagtc
     7441 gatctctgcg ccaatcgact tgccgatcca attgcgctgc atggtcagca ctttttccgg
     7501 ccaaccactt aactggtcga gatcatcaag cagctcgtcg gcataatcgg tgatgcggaa
     7561 aaaccactgc tccagttctt tttgcacgac cgtcgaatcg cagcgccaac aggcgccgtc
     7621 gatcacctgt tcgttggcga gcacggtttc acaggaagga caccagttga cgaacgagct
     7681 cttcttgtac gccagcccct tcttgtagaa ctgcaaaaaa atccattgat tccagcgata
     7741 atattccggc tggcaggtgg cgatctcgcg ttcccaatca taggagagac ccatgcgttt
     7801 cagctgagtc ttcatgtaga gaatattttc ctgggtccaa atctccggat gcactttacg
     7861 ctcgattgcg gcgttctcgg ccggcaaccc gaacgcatcc caacccatcg ggtggagcac
     7921 gttgaagccg cgcatacgct tgtagcgcgc gaccacgtcg ccgatggaat agttgcgcac
     7981 atggcccatg tgaatccgac ccgaaggata gggaaacatc tctagaacgt aatacttggg
     8041 tttgcccgga tcctcgattg ccctgaacgc ctgctcgtcg gcccaaagct tttgccattt
     8101 gtcttcgatc gattgcgctt gatagcgttc gttcatggcg gaccgctact cagatctcgc
     8161 gcggctcgtc tgcgcgattt gatcgagcac gccattgata aaagcggcgg aatcgtcact
     8221 gccgaatctt ttggcgatct ccaccgcctc gtcgagactc acggcatcgg gaatatccgg
     8281 acagaatgcc acttcgtatg ccgccaagcg caggatctcg tgatcgactt tggcgagccg
     8341 ttccattttc cagtgttccg tcgactgttg gatcaagcga tcgagctccg gccgctggct
     8401 caccacaccg gataccagac ggcgtgcaaa ctctttggcc ttagcgccgc cttcaaaatg
     8461 ttgaagaaac aaatccacgg cggccattga agagtcaccc gtgatctcaa tttggtaaag
     8521 cgcttggaga gccagctcgc gccctttgcg gcgcatcgta cgcggggcgg cgggcgtgtt
     8581 gtccgatgaa tcctgtggca atggtcgctc gaatgggtta ctggcgggaa acacaaaaag
     8641 ttaagacacc tggcgaagca cgttgaccat ttcgacagcg accagcaagg cttcataacc
     8701 cttattggcg tccttgctgc cggcgcggtc catcgcttgc tggacattgt cggtggtcaa
     8761 aacgccgaag ccgacgggaa tcttgtgagt cttgactgcg tcacccaatc cttgggtgac
     8821 cgcgtcgcag acgtagtcga agtgaggcgt atcgccgcgg atgaccgcgc cgaggcaaac
     8881 cagcgcgtcg tatttgccgc tagtcgccat cttatccgcg acgatcggta tttcgaacgc
     8941 ccccggtacc cgaaccacgg tcaaatcatt ctccgcgcca ccgtggctgc taaaaccgtc
     9001 cagtgcacct tcgagcaacc gctccgtgac aaacccgtta aaacggctga cgataatacc
     9061 gatcttcaac ccctgcgcat ccggttttcc ttcgagaata cgggccatat agattaacgt
     9121 gtgcggcgct aagcgctcag cgcttggcgg ttctatcttc gagattggac aacatgtggc
     9181 cgagcttgcg ctgtttggtg cgcaggtaat ggatgttggt cttgagcggc tgggcttcga
     9241 gcgcgacgcg ttcgaccact ttcacaccgt aattttccaa accggaaatt ttgcgcggat
     9301 tgttagtcaa caacgaaacg ttttcgacat gcaggtcgcg tagaatttgc gcgccgatgc
     9361 cgtaatcgcg caggtcctct ttgaacccca actcgagatt ggcctcgacg gtgtcgcgcc
     9421 cctggtcctg cagtgcgtaa gctctgattt tgttggtcaa gccgatgccg cgaccctctt
     9481 gatgcatata gaccagaatg ccgcaaccga gctcgtcgat gcggcgcaaa gattcacgaa
     9541 tttgatcgcc gcaatcgcag cgctcggagc caaagacgtc accggtcaga cattcggagt
     9601 gcaagcgcac caagacgggc gctgctttag taatatcacc tttaaccagc gccatatgct
     9661 ccgcgccatc gacgacgtta cgatagacga tagctttgta ttttccgccg tgcaaagtgg
     9721 gaaactcttc ttcggcaacg cgcaggacca aactttcatt gcgcagccgg tatgcaatca
     9781 aactagccac cgagagaatt tttaagccat gcttttcggc cagccgttcc aatgtcggta
     9841 gggcggccac cgaaccgtct tcgtccagta tttggcagat cactccagcg ggcttgaatc
     9901 cggcaagccg ggaaagatcc accgaagcat cggtgcggcc ggatcgagcc aacaccccgc
     9961 cgctacgggc ctgcagcggc tgcacatgcc ctggcaccat cagatcctca tgcttagtgg
    10021 cgtcggcgat cacggagcga atcgtgtgcg cccga
//
LOCUS       DATLCH010000364        10185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8937373, whole genome shotgun sequence.
ACCESSION   DATLCH010000364 DATLCH010000000
VERSION     DATLCH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8937373"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VMZ02_10880"
     CDS             complement(<1..477)
                     /locus_tag="VMZ02_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HUR72483.1"
                     /translation="MAYSLKQFIRDLTSITERYHEQEKIVAVAKPLLAELVNNPDCIE
                     AQFKRHGGKTYGRYMLHRAQGFNVTSVVWGPGDGAKAHNHDTWGLVGVVENELQETRF
                     TRRDDGGKEGYADLTATSITRNKAGMVSTLIAPENDIHEMLNATQKNTVEVHVYGKD"
     gene            complement(553..1605)
                     /locus_tag="VMZ02_10885"
     CDS             complement(553..1605)
                     /locus_tag="VMZ02_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72484.1"
                     /translation="MAFVSDLIISSDSHVMEPVDLWKKGVSEKYRDAAPLFPPHQVGE
                     GFQQHAGGWDPKARMREMEADGLSAEVLYPTLGLDLFGIDDAGLQEECFRVYNDWLID
                     YCSADPTRLIGIPAVAVYNVDNGIKELERCHKAGLKGAIIWQAPHKDIPFTSDHYEKF
                     WAACANLDAPVSLHILTGHSYNKDKNRKGVERYRGSVNLKLMDIANALFELIFCGVFE
                     RHPKLKVVSVENESGWMPWIFQQWDYYYNRFKKENPPAFSRNPSSYIKEQIFACFFND
                     HVCGENLAFWGQDNIMWSNDFPHPNSTWPNSIKIIQRDLGHLPEETQRKVVCDNVCRL
                     YDIDASKLPRVQKQAA"
     gene            complement(1790..2431)
                     /locus_tag="VMZ02_10890"
     CDS             complement(1790..2431)
                     /locus_tag="VMZ02_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HUR72485.1"
                     /translation="MTVKLSKEELAKASAVTVGHYDQRADVFWRDTRDHDVSQNIEAL
                     LQALVGTPPFRILDFGCGPGRDLKSIKDLGHTPVGLDGSARFVEMARELTGCEVWHQD
                     FFKLELPADYFDGIFANASLFHVPSQELPRVLKELYATLKPDGVLFSSNPRGDNREGW
                     QGQRYGVYIDWPTWRELVTGAGFGEIRHYYRPTGLPREQQPWLASVWRKPARE"
     gene            complement(2478..3467)
                     /locus_tag="VMZ02_10895"
     CDS             complement(2478..3467)
                     /locus_tag="VMZ02_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HUR72486.1"
                     /translation="MASTPFKVAILDDFEKIADTVPSYAKLKARAEITLLRERLDSSE
                     KIVQRLQEFDALLLMRERTFLSDNEYSQLPKLKFISQTGRTSVHLDLANATKRGIVIA
                     STPADSGAATKELTIGLILSLVRKIPQINLRMREELWPAATSTLLEGKTVGVLGLGRI
                     GREVARVMKAFNTRVLGYSRTLTADKAAEVGAEAVSLETLLRESDIVTLHVHFGEQTR
                     GLIGEKELALMKRGAFLVNTGRGPLVVEQAMLNALKSGQLGGVGLDVYDIEPLPMDHA
                     LRRFDNAILMSHRGYTTIEILRERYEQAITNILDYLDGKEIKLINPDVVARRP"
     gene            complement(3472..4152)
                     /locus_tag="VMZ02_10900"
     CDS             complement(3472..4152)
                     /locus_tag="VMZ02_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72487.1"
                     /translation="MRKRIFSWLGHEFTEISGEAAPEADAAIEARELFRRFDHELRAD
                     GLSLANTVRSRLWGRDRASRDGGSNERVKALSGKARSASSSYIAPDHFDSPAKVAIDL
                     IAMRPSQAEQAKQIVEYDPPIVPIRFLVYDSVVVLSGVTTVLPTLAEQFDNILPRISG
                     SLADAGSSWAKVAQVSFYFHRSQELANLKKLFSDRVSAQIPRLEYIVVDGYSAEGKFC
                     EVEVTATR"
     gene            complement(4242..4388)
                     /locus_tag="VMZ02_10905"
     CDS             complement(4242..4388)
                     /locus_tag="VMZ02_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72488.1"
                     /translation="MLKKSGGLIFFFIMIIAGLGIANFLSMTFYEKSLLTAIKDFVFV
                     VTKR"
     gene            complement(4483..6027)
                     /locus_tag="VMZ02_10910"
     CDS             complement(4483..6027)
                     /locus_tag="VMZ02_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HUR72489.1"
                     /translation="MRRIVIGLVIIALFTLATGPATAQDQIRDLVVKIHAVQQAPDLL
                     RPWARNSPQQVKGSGVVIDGRRILTNAHVVRYASQIYVQPNQSASHIPARVEAMTAGM
                     DLAILKLDDETFFENRGALPFAQELPRVKDSINVYGYPTGGTELSVTQGIVSRIEFTD
                     YYYQATGLRIQVDAALNFGNSGGPAVSDGRLVGLVFSLIQNAQNIGYLIPVEEIQHFL
                     GDVKDGVYDGKPQTHDLIQTIENDALRQRLGLPKGVNGVMVAQPYRDNPEYPLKEWDV
                     ITKIGDTPIDADGKVAIRYDLRLSASYLVQKYAKNGLLPLTVFRKGDLLSVNLPVGSQ
                     RELVIPYLLDANPRYFIFGPFVFSQTTQDYLERLGNQRPGAFGRRPSPLVIRRQDRPA
                     FDNEELVVVASPLFPHRITKGYDDPNRSVISEINGVPVRNLRHLVELLRDNRDEQVSF
                     KFASSGVLTHETMVFNRKDLLDATGKILEENGIRYPYSQDLRAIWEAAPTGNKSAQVT
                     CCGLTP"
     gene            6164..7027
                     /locus_tag="VMZ02_10915"
     CDS             6164..7027
                     /locus_tag="VMZ02_10915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HUR72490.1"
                     /translation="MSSEVTALLAAISFALFAVYGWLGLRHSTPLTATIVSLVARTIT
                     LGSAVILFGGVPGFALKALVVFIILGLMQTVISLLTFVGLQKIGTSRSQPLRNSYPLW
                     SSVIAITLLGERAGGAVLLGTLLVVVGVVMISWKPETAPPNYRWWHIVYSLLAGFLAG
                     VAFPLRRYGLTITNEPVFFSFIVAIVSVIGTIPYTLRTAGERKLIWHRRAVGDFFLSG
                     FFEALGALFTLLALTSGRVVIVSPIVATTPLFSLLLSLLFLRGKEKITALTVLGTIAV
                     VGGTIAIALWR"
     gene            7109..7414
                     /locus_tag="VMZ02_10920"
     CDS             7109..7414
                     /locus_tag="VMZ02_10920"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HUR72491.1"
                     /translation="MWYLVLRQATKPREQWTVTLDQHLVWMKRQHDAGKILFSGPTTD
                     RKYGIYVIRADSKDEASRIAGSDPYTAAGFCSFELLEWEIHQIMGAGNFTAAGLGHK"
     gene            7451..8578
                     /locus_tag="VMZ02_10925"
     CDS             7451..8578
                     /locus_tag="VMZ02_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72492.1"
                     /translation="MRRSIRCGVFVVLAGCINFSVLTAADAQSDWKKQWEATIEAGRK
                     DGEVVIYGPHNPAYQHVWAIFQKLYPEVKFNFVPGKGSDHVQRIVAERRAGKYLADLI
                     MGGSSTYAAFTPGTLEPLKPLLILPDVADASNWFDGRFHFADPQNNAGLIISGEIGTR
                     RGSYNTKLLDPKEVHSWWDLLKPQFKGKLVTFDPRVAGGGGETFMFFYYTPTLGQNFI
                     TRILSESDILITRDLQQGTDWLGQGKVLFQIGSGQPVMKANKQGLPVDLMPHAMKEGD
                     IMGGGSCCLAVMSKAPHPNAAKLFLNWVLSREGQSAWQKYTEVNSLRVDIPKSDLPAD
                     DVPQKGVNYFMLNSAKYNGMEGRKALHQIVEDALKKAGKAK"
     gene            8685..9638
                     /locus_tag="VMZ02_10930"
     CDS             8685..9638
                     /locus_tag="VMZ02_10930"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR72493.1"
                     /translation="MEKIGLIGLGNAGRPMAQRILSAGYRLTVFDIDAAAVAAVARLG
                     AQSASTAAQAVGDITITLVPSSVEVRSAVFGSDGAFEALQPGMALVDLSGTDPDCARE
                     LQERLQEKRATFIGGTIHASGAPAVVIPTGQFAIVIGGTKNQIEPTAKLLSAIAQTIV
                     CLPEPWMPKAFKIAIILYATTNNIITAEICSWLTAQGADPKLFLQLMQSTGSPASAAR
                     MEEFMKRNNTNGGALSNSYKDFRQALNIAANLEIPMPLTSMANQIQEMGRAAGFSRFN
                     SPAAMGKLYELLTGEDLSGATLRSEKRIPESREPQVIWLGD"
     gene            complement(9678..>10185)
                     /locus_tag="VMZ02_10935"
     CDS             complement(9678..>10185)
                     /locus_tag="VMZ02_10935"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72494.1"
                     /translation="AIVMGGITLGTQRGYAVEQEVIATLSRQVGLPVSTGMSATVQAL
                     KHLKVQSTVIATAYKASINQAVQKYYEDGGIKVASIKGMEVAKPVDQVKLPDYSSYKV
                     ARELWRENPGVESLLLQGRWRTVAYVQELENDTQLPVVASTAAALWWVMQQLRIKAPV
                     PGFGRLLA"
BASE COUNT         2126 a         2685 c         2992 g         2382 t
ORIGIN      
        1 atctttgccg taaacgtgaa cctccacggt atttttctgt gtggcgttga gcatctcatg
       61 gatgtcgttt tccggcgcta tcagcgtcga gaccatgccc gctttgttgc gagtgatcga
      121 ggttgctgtt aggtcggcgt agccttcctt gccgccgtcg tcgcgccggg tgaagcgggt
      181 ttcttgcaat tcgttttcga ctacgccgac caacccccac gtgtcgtgat tatgcgcctt
      241 agcaccatcg ccggggcccc agacgactga ggtgacgttg aagccttgcg cgcgatgtag
      301 catgtagcgg ccataggtct tgccgccatg gcgtttgaac tgcgcttcga tgcaatcggg
      361 attgttgacg agctcggcaa gcagtggttt tgcgacggca acgattttct cttgctcgtg
      421 gtagcgttcg gtgatgctgg tgagatctct gataaattgt tttagcgagt aggccatcag
      481 tgggtgtgct ccaaatgcat aggggcgggt aataaacccg cccctatcga ctgttatgtt
      541 tattcagtgt tttcaggccg cttgtttttg cacgcgcggc agtttcgagg catcgatatc
      601 gtagagccgg cagacgttgt cgcaaaccac tttgcgctgg gtttcttccg gcagatgacc
      661 gagatcgcgt tgaataatct tgatcgagtt cggccacgtc gaattgggat gcgggaaatc
      721 gttggaccac atgatgttgt cctggcccca gaaggcgagg ttctcgccgc agacgtgatc
      781 gttgaaaaag cacgcgaaaa tctgctcctt gatgtagctg cttgggttgc gtgaaaaggc
      841 cggcggattc tccttcttga agcggttgta gtaatagtcc cattgctgga agatccaggg
      901 catccagccg gattcgtttt ccacggatac gactttgagc ttgggatgtc tttcgaagac
      961 gccgcagaag attagctcga agagcgcatt ggcgatgtcc atgagcttca agttgacgct
     1021 gccgcgatag cgctcgacgc ccttgcggtt tttatcttta ttgtagctgt gaccggtcag
     1081 gatgtgcagg ctaacgggcg catcgagatt ggcgcaggcg gcccagaact tttcataatg
     1141 atcggaggtg aacgggatat ctttgtgcgg tgcctgccag atgatcgcgc ctttcaaccc
     1201 ggccttgtgg cagcgctcga gttctttgat accattgtcg acattgtaaa ccgcgactgc
     1261 aggaatgccg atcagccggg tcgggtcggc gctgcaatag tcgatcagcc aatcgttgta
     1321 gacgcgaaag cattcttcct gcaaaccggc gtcgtcgatg ccgaacagat caagacccaa
     1381 ggttggataa agcacttcgg cgctcaagcc gtcagcttcc atctcccgca tccgtgcttt
     1441 gggatcccaa ccgccggcat gttgctggaa gccctcgccg acctgatgcg gcgggaagag
     1501 cggcgcagcg tcgcggtatt tttccgacac gccttttttc cacaagtcga ccggttccat
     1561 aacatgagag tcggatgaga tgatcaggtc gctaacaaaa gccattggac gatcctcctt
     1621 atcggattag gtctaagata tagggcacct taatagatac gcggcgctac tgtcaattag
     1681 tccaccgccg tctggagcga cggcgtgatg gagtcatggg atggcgggat ctaagcttta
     1741 tcttaggact acgctttact tacaagactc cagcgctcca ctactccagt cattcccgcg
     1801 ctggtttgcg ccagactgat gccaaccagg gttgctgttc acgcggtagg ccggtcggcc
     1861 ggtagtagtg gcggatttct ccaaagccag cgccggtcac cagttcgcgc caggttggcc
     1921 aatcaatgta gacgccatag cgctgacctt gccagccttc ccggttgtcg ccgcgcgggt
     1981 tggagctgaa cagtacgccg tccggtttca atgtcgcgta gagttctttc aatacgcgcg
     2041 gcagttcctg gctcggtacg tgaaagagcg aggcgttggc gaagatgccg tcgaagtagt
     2101 cggcgggcaa ttcgagctta aagaagtcct gatgccacac ttcgcagcca gtgagttcgc
     2161 gcgccatttc gacgaaacgc gccgaaccat cgaggccgac aggcgtgtga cccaaatctt
     2221 ttatagactt caagtcgcgc ccgggaccgc agccgaagtc gagaatgcga aacggcggcg
     2281 tgccgaccaa agcttgcaac aacgcttcga tgttttggct gacgtcatgg tcgcgcgtgt
     2341 cgcgccagaa aacgtcggcc cgctgatcgt aatgaccgac ggtgacggcg gatgccttgg
     2401 cgagttcttc cttcgatagt ttgacagtca caatggcgga acttgagagc agttcgatcg
     2461 attgacggtc gtttagctca cggccggcgc gcgaccacat ctggattgat cagcttgatt
     2521 tctttgccgt cgagatagtc cagaatattg gtgatcgctt gctcgtaacg ttcgcgcaag
     2581 atttcgatgg tggtgtagcc acggtgggac atcaggattg cattatcgaa gcggcgcaac
     2641 gcgtggtcca tcggcaacgg ttcgatgtcg tagacgtcga gtccgacgcc acccaattgc
     2701 ccgctcttga gcgcattcag catcgcttgt tccaccacca gcggaccgcg cccggtgttg
     2761 accagaaatg ctcctcgctt catcagtgcc agctcttttt cgccgatcaa gccacgggtt
     2821 tgttcgccga agtgcacatg gagggtgacg atgtctgatt cgcgcagcag ggtttccagc
     2881 gagaccgcct cggcgccgac ctcggcggct ttgtcggccg tcaacgtacg cgaatagccc
     2941 agcacgcggg tattgaaagc tttcatgacg cgcgcgactt ctctgccgat gcgaccgagg
     3001 cccaacacac cgacggtttt gccttcgagc aaggtgctgg tggccgccgg ccagagttct
     3061 tcgcgcatgc gcaagttgat ttgcggtatt ttccgcacca gggacaggat caaaccgatg
     3121 gtgagctcct tggtcgccgc gccgctgtcg gcgggagtgc tggcaatcac gataccgcgc
     3181 ttggtggcat tggcgagatc aagatggacg ctggtgcgac cggtttgtga aataaattta
     3241 agcttcggca gctggctgta ttcgttgtcg cttaaaaaag ttcgttcgcg catgagcagc
     3301 agggcgtcga actcttgtaa acgctgcacg atcttttcgc tggaatcgag ccgttcgcgc
     3361 agcaaggtta tttcggcgcg cgccttgagc ttggcgtagg acggtacggt gtcggcaatc
     3421 ttctcgaaat cgtcgagaat ggcgaccttg aaaggcgttg atgccatgga cctacctcgt
     3481 cgcggtgact tccacttcgc aaaactttcc ctcggcggag taaccgtcga cgacgatata
     3541 ttccaatctg gggatttgcg cggaaactcg gtcggagaaa agttttttca gattggccag
     3601 ttcctgactg cggtgaaagt aaaacgagac ctgcgcgacc ttggcccacg aactgccggc
     3661 atcggccagg gaaccactga tgcgtggcaa aatattgtcg aactgctcgg ccaatgtcgg
     3721 cagaaccgta gtgacaccag acagcaccac cactgaatcg tagacaagaa agcggatggg
     3781 cacgatgggc ggatcgtact cgacaatttg ttttgcttgt tccgcctgag atgggcgcat
     3841 ggcgatcagg tcgatggcaa ctttggccgg agagtcgaag tggtccggtg cgatatagct
     3901 cgagctggcg gagcgggcct tgccggagag cgcctttacg cgttcgttgc tgccgccgtc
     3961 ccgactggcg cggtcgcgtc cccacaatcg gctgcgcacg gtgttggcga gggagagccc
     4021 gtcggcgcgc agttcgtggt cgaagcggcg aaacagctcg cgcgcttcga ttgcggcatc
     4081 cgcttccggc gcagcttctc cagaaatctc ggtaaactca tggccgagcc atgagaagat
     4141 tctcttgcgc ataaagatcc tttctcgatg tgcgcgcgat cctagtacac gctggagcgc
     4201 ggatcaatga caattctacg ctagctcgcg aaggtgggca tctacctttt tgtcacaaca
     4261 aacacgaagt ccttgatcgc tgtaagcagc gacttttcgt aaaatgtcat cgacaagaag
     4321 ttggctatgc cgagcccggc gatgatcatg atgaaaaaga atattaaacc gccggatttt
     4381 ttcagcatct gttccctcgc ctaatcgatc gaagccacac gcctcgcttg ggtccccgga
     4441 tgcggctgtc cgttgcaatt catcgatgag ttttatagac ggttacggcg taagtccgca
     4501 gcaggtgact tgcgccgatt tgttgccggt cggagccgct tcccaaatcg cccgaagatc
     4561 ctgggagtag ggataacgga tgccattttc ttcgagtatt ttgccggtgg catccaagag
     4621 atccttgcga ttgaacacca tggtctcatg ggtcaagacc ccggacgagg cgaatttgaa
     4681 actgacttgc tcgtcgcgat tgtcacgcaa gagttcgacc aaatggcgca gattgcgcac
     4741 tggaacgcca ttgatttcac tgatcaccga acgattgggg tcgtcatagc ctttagtaat
     4801 tcgatgggga aacagcggcg acgcgacgac gacaagctcc tcgttatcga atgccggccg
     4861 gtcttgacgc cggatgacca atggacttgg ccgccggccg aacgcaccgg gccgttggtt
     4921 gccgagtcgt tcgaggtagt cctgggtcgt ctgcgaaaat acgaacggcc caaaaatgaa
     4981 gtaacgcgga ttggcatcca acagatatgg aatgaccaac tcccgttgag atcccaccgg
     5041 caaatttacc gacaatagat cgcccttgcg aaacacggtg agcggcagca aaccgttctt
     5101 ggcgtacttt tgcaccaggt aggacgccga aagccggaga tcgtaacgga tggcgacttt
     5161 tccgtcggcg tcgatgggcg tatcgccgat tttggtgatg acatcccatt ccttcaacgg
     5221 atattccgga ttgtcccgat aaggctgcgc aaccatgacg ccattgacgc ctttaggtaa
     5281 gccaagacgc tggcgcaagg cgtcgttctc gatggtctga atcaaatcgt gagtttgcgg
     5341 tttgccgtcg taaacgccgt ctttaacatc gccgagaaaa tgctgaattt cttcgacggg
     5401 aatcaaatag ccgatgttct gcgcattctg tatcagacta aataccaacc caaccagtct
     5461 accgtcggaa accgccggtc cgccgctatt gccgaaattc agcgcggcgt cgacctggat
     5521 gcgtaaaccc gtggcttgat agtagtagtc ggtaaattcg atgcgcgaga cgatgccctg
     5581 ggttaccgaa agttcggtgc cgccggtggg atagccatag acgtttatgc tgtctttgac
     5641 acggggcaac tcctgtgcga atgggagagc gccgcggttc tcaaaaaacg tttcgtcgtc
     5701 taatttcaaa atcgccaaat ccattcccgc ggtcatcgct tcgacgcgcg cgggaatatg
     5761 gctggccgac tgatttggtt gcacatagat ctgactggcg tatcgcacta catgggcatt
     5821 ggtgaggatg cgccgtccgt cgatgacaac tccggagcct ttcacctgct gtggactgtt
     5881 gcgagcccat ggccggagta gatcaggggc ttgctgaacg gcgtgaattt tgacgaccag
     5941 atcgcgtatt tgatcttgcg ccgtcgccgg gcctgtcgcc agggtaaaga gcgcgataat
     6001 gaccaatccg atgacgattc ggcgcatggg ttctccttta ataataaaac tcagagctca
     6061 attgggtctc tgattatata cgaaaagctg gcttataagt aagatacttc tttattgatc
     6121 gttttaattg attgcaacac tctagtcttg aacaaggttt cttatgagtt ccgaagttac
     6181 cgcactgctg gcggcgatct cctttgccct ttttgccgtc tacggctggc tcggtctgcg
     6241 ccattcgact ccgctgaccg ccactatcgt ttccttggtc gcacgaacga tcactctcgg
     6301 cagcgcggtg atcctgttcg gcggggtgcc cggattcgcg ctgaaagcgc tggtcgtctt
     6361 cattatcctc ggcctgatgc agacagtaat cagcttgctt accttcgtgg ggctgcaaaa
     6421 gatcggcact tcccgcagtc agccgctgcg caacagctac cctttatgga gctcggtgat
     6481 cgccatcacg ctgttgggcg aacgcgccgg tggcgcggtc ttactcggca cattgctcgt
     6541 ggtcgtcggt gtggtgatga tttcctggaa accggagacg gcgccgccga actatcgctg
     6601 gtggcatata gtctactcgc tgctggcggg atttctcgcc ggcgtggcgt ttcccttgcg
     6661 ccgatacggt ctgacgataa ccaatgagcc ggtgtttttc agcttcatcg tcgcgatcgt
     6721 ttcggtgatc ggcacgatcc cgtacacctt gcgcacggcg ggcgaacgaa agttgatctg
     6781 gcatagacgc gcggtgggcg actttttttt gtccggtttc ttcgaagcgc tcggcgcctt
     6841 attcacgctg ctggcgctga caagcggccg ggtcgtcatt gtttctccga tagtcgcgac
     6901 tacgccgctg ttcagtttgc tgctgtcgct gctattcttg cgcggcaagg aaaaaattac
     6961 cgcgctcacg gtgctcggca ccatcgcggt ggttggcggg acgattgcaa tcgcgttgtg
     7021 gcgataaata ttcttgacag gcaaagctcg cgagcataac gattggaaca tcattctcgt
     7081 tctaatctgg aatgcgacag gaggcagcat gtggtatcta gttttgcgcc aggcgaccaa
     7141 gccgcgcgag cagtggaccg tgactttgga tcagcatctg gtttggatga aacgacagca
     7201 cgacgccggg aagattctgt tttccggacc gacgaccgat aggaaatatg gaatctacgt
     7261 gattcgggcg gattccaagg atgaggcgtc gcggattgcc ggcagcgatc cctatacggc
     7321 ggcgggcttt tgttccttcg aactgctcga atgggaaatc catcagatca tgggcgctgg
     7381 caattttacc gcggcggggt tggggcacaa atgacgaatt ggagtgttgg tgtcatggag
     7441 tgatctggaa atgcgaagat caatacgatg cggagttttc gttgttcttg ccggttgcat
     7501 aaatttctcg gtgttgactg ctgctgatgc tcagtcggat tggaagaagc agtgggaggc
     7561 gacgattgaa gcggggcgga aagacggcga ggtcgtgatc tatgggccgc ataatccggc
     7621 gtaccagcat gtctgggcga tttttcaaaa attgtatccg gaggtgaagt ttaattttgt
     7681 gccgggcaag gggtcggacc atgtgcagcg catcgtcgcc gagcggcgcg ccggcaagta
     7741 tctggcggat ctgatcatgg gagggtcgtc gacctatgcc gcatttacgc cgggcacttt
     7801 ggagccgctc aagccgctgt tgattctgcc ggatgttgcc gacgccagta attggttcga
     7861 tggccgattt catttcgccg acccacagaa caacgccggg ctgatcattt ccggtgagat
     7921 cggcacgcgc cgcggttcgt ataacacgaa gctgctcgat cccaaagaag ttcactcctg
     7981 gtgggatctg ctgaaacccc agtttaaagg caagctcgtg actttcgatc cgcgtgtggc
     8041 aggcggcggt ggcgagactt ttatgttttt ctactatacc ccgacgcttg gccaaaattt
     8101 cattacacga attttaagcg aaagcgatat tctgatcact cgcgatcttc aacaggggac
     8161 tgattggtta gggcaaggca aagtgctgtt tcaaatcggt agcggccagc cggtcatgaa
     8221 ggcaaataag caaggcttgc cggtggacct gatgcctcat gccatgaaag aaggggacat
     8281 catgggtggc ggttcctgct gtcttgcagt gatgagcaag gcgccgcatc ccaacgccgc
     8341 aaagctgttt ctcaattggg ttttgtcccg cgaaggacag agcgcctggc aaaaatacac
     8401 cgaggtaaat tcgctccgag tggacattcc caagagcgac ttgccggccg acgacgtgcc
     8461 gcagaagggc gtgaactatt tcatgctcaa ttcggcgaag tacaacggca tggaaggacg
     8521 taaggcgctg catcagatcg tcgaagacgc gctgaagaag gcaggcaagg cgaaatagcc
     8581 gctctctgat tcgcccgaat ccaggtattc atcggattcg aaactctcgg caacgctata
     8641 tttgccgagg cgtttgaaga cgcaatgcgc cagagagatt gagtatggaa aaaattggtt
     8701 tgattggatt gggcaatgcc gggcgaccca tggcgcagcg cattttgtcg gcgggctacc
     8761 ggctaactgt atttgatatt gacgccgcgg cggtagcggc ggtggcgcgg ttgggtgcgc
     8821 agagtgcgag tacggcggcg caagccgtgg gcgatatcac aattacattg gtaccgtctt
     8881 cggtggaagt gaggagtgcg gtatttggct cggacggcgc cttcgaggcg ttgcagcccg
     8941 gcatggcgct cgtcgatctg agcggcaccg atccggactg cgcccgcgag ctgcaagaac
     9001 gcttacagga aaagcgggcg acctttatcg gcgggacgat tcatgccagc ggcgcgccgg
     9061 cggtggtcat tccgactggg cagttcgcca tcgtcattgg cggtacgaaa aatcaaatcg
     9121 agcccacggc taaacttttg agcgcgattg cgcagacgat cgtctgcttg ccggaaccct
     9181 ggatgccgaa ggcgttcaag atcgcaatta ttttgtatgc aaccaccaat aacattatca
     9241 cggcggaaat ttgttcgtgg ctcaccgccc agggagccga tcccaaattg tttctgcaac
     9301 tcatgcagag caccggctcg cccgcttcag cggcgcgaat ggaagaattt atgaaacgca
     9361 acaataccaa tggcggagcg ctcagcaata gctataaaga tttccgccaa gcgttgaaca
     9421 ttgctgcgaa tctcgagatc cccatgccat taacctcgat ggccaatcaa atacaggaga
     9481 tgggccgcgc tgccggcttt agtcgtttca acagcccggc ggcaatgggt aaactctacg
     9541 agctccttac cggagaagat ctcagcggcg caacactgag gtctgaaaaa cgcattccag
     9601 agagccgaga gccacaagtg atttggctag gtgattgatg tcgggtgggt gggcacgtgc
     9661 tgcgtcattg ctaagtttta agctaacaag cgaccgaatc cgggaaccgg ggctttgatg
     9721 cgcagctgct gcatcaccca ccaaagcgcc gcggctgtcg aggcgaccac cggcagctgt
     9781 gtatcgtttt ccaactcttg cacgtaggca accgtgcgcc agcggccttg cagaagcagg
     9841 ctctcgacgc cgggattttc tcgccagagt tcgcgggcga ctttgtaaga cgagtagtcc
     9901 ggcagcttta cctggtccac cggtttggca acttccatcc ctttgattga cgccactttt
     9961 atgccgccgt cttcatagta tttctgcacg gcctggttga tcgatgcctt ataagccgtg
    10021 gcgattacgg tcgattggac ctttaagtgc ttgagtgctt gcacggtcgc gctcatcccg
    10081 gtgctgaccg gcaggccgac ttgacgcgat agcgtggcga ttacttcctg ctcgacggca
    10141 tagccacgtt gggtaccgag agtgattccg cccatcacga tcgcg
//
LOCUS       DATLCH010000365         4530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6509797, whole genome shotgun sequence.
ACCESSION   DATLCH010000365 DATLCH010000000
VERSION     DATLCH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6509797"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VMZ02_10940"
     CDS             complement(<1..942)
                     /locus_tag="VMZ02_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72495.1"
                     /translation="MILGDWRSGLAVLAGVVALCSPQPAVQAQTQKIRISLSSRSNTN
                     TSYYVAQARGFFKDEGLEVEFIQINPRLGALAVLNGDVTYTTSFVSTFRGVVQGLPMK
                     TVLILLKKGVYYLMVRPEVIKDVQELKGKKLGVTAINGGDHIIGRELLRMKGIDPNTV
                     QAIAVGDPSLRFQAVMTGIVQAVSVPPPYDVQLQDHGLKAVSGPPEIGVPSSGLFVAD
                     RLIKENPLILRKTIRAALRANRFIEANREETIRIMAQYVPQKLDLAGRSYDVELKALA
                     KDGTMTDAEIEFQMDRLAEKRRPLDEVRDFSFARQAMK"
     gene            1156..2184
                     /locus_tag="VMZ02_10945"
     CDS             1156..2184
                     /locus_tag="VMZ02_10945"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72496.1"
                     /translation="MKLKLGIIVLLFSSLLRLNALANPYLPKSGEKPFPLRVATSAVT
                     GGFIHLYAALDYGILEKYGFKPEHIYIRGQSPALAALAADQTQFNYGAADGSLPGLAA
                     GVDAKFVASPLVKLPYVMVARKDIRRPEDLKGKSIGVTRPGDLSARLSRQVIRKFGLT
                     TDDVTIHPIGGSQQERYQAMVGNVVQAILVTPPLDVRAKSDGFNVIYRLIDLEIPFVY
                     SSLMTNYKMLRERPETVQRMVAALAEAVYFVEKNPDKAKAAVAKAMRVKDEEALQTSY
                     NVYARDIVDRTMVVPGKSITEAWELARDTGTIVRRKPEELYDNSFVTNLEKSGFLKEL
                     WGNENYRK"
     gene            2230..3252
                     /locus_tag="VMZ02_10950"
     CDS             2230..3252
                     /locus_tag="VMZ02_10950"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72497.1"
                     /translation="MKIKLFALIVILLSSAPAGANPYLAKPGERTLSMRVSTCAVSGG
                     FIHLYTALDNGLFDKYGVKLENIFIRGSAQNLTALATDEVQFTYCASDAIIPGMAAGL
                     EVKIVASPLVKLPYVLVTRKDVRRPEDLKGKALGIARAGDLSDRLSRAVVKKLGLSPE
                     AVTIRPIGGSQGERYQAMAAGIVHGVIITPPLDVRAKNEGYNVMYRLIDLNIPFIYSS
                     LHTSYKMLRERPEIVQRLVAAFAEIIHFVEKNPDKAKAAVSKAMRTNDAEALQSAYDT
                     YAREILDRTMVVPGKAVAETIEIARESGSPVRKKPEEIYDNSFVNNLEKSGFMKEIWG
                     NENYRR"
     gene            complement(3330..4238)
                     /locus_tag="VMZ02_10955"
     CDS             complement(3330..4238)
                     /locus_tag="VMZ02_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72498.1"
                     /translation="MPAAHGASAPTRVTIHVPSKSLSIMPYYLGKDKGFFAAEQIEPQ
                     LIMMAPPTAIAAMVAGELDFSSTTGAATSAIMRGLPLRRVFYVQQDPVHVLISQPEIK
                     SVQDLVGKAIGVTALTDAVGMSANVILRAHGIEAGRVTLLAMQVTDNAIKSLTSKRIA
                     ATLLAPPYVEELEAKGYVKIAEARTYAPLSFIGLVSSMETMKKSPQKVQSMVSALHRT
                     MNYILNPANRNEVVQYVATYHKLDLGLAEKAMAAQMLGYSKDGTKPRAAVEKEIEIYR
                     ESLKVAKAFTPDDLEDMSWLRKVQVK"
BASE COUNT         1011 a         1323 c         1213 g          983 t
ORIGIN      
        1 tttcatcgct tgacgggcaa atgaaaaatc acgcacttca tcgagcggcc ggcgtttctc
       61 cgccagtcgg tccatttgaa actcgatctc ggcgtcggtc atggtgccgt ctttggccag
      121 ggctttgagt tcgacatcgt aggaacgccc ggccaaatcc aatttttgcg gcacatattg
      181 cgccatgatc cgaatcgtct cctcgcgatt ggcttcgatg aatcgattcg cccgtaaggc
      241 cgcgcggatg gtctttctca atattagggg attctccttg atcaaacggt cggcaacaaa
      301 cagtccggac gacggcacgc cgatctctgg cggccccgag accgccttca agccatgatc
      361 ttgcaactga acatcgtaag gcggcggcac agatacagct tggacgatgc cggtcatgac
      421 cgcttgaaag cgcagcgatg gatcgcccac cgcgatcgct tgcaccgtgt taggatcgat
      481 tcccttcatg cgcaataatt cgcgcccgat aatgtgatcg ccgccgttga tggccgtcac
      541 accaagcttc ttgcccttga gctcttgaac atccttgatg acctccggcc gcaccatcag
      601 gtaataaact ccttttttga gcaggattaa tactgtcttc atcggtagcc cctgaacgac
      661 gccgcgaaag gtgctgacga atgaagttgt gtaggttaca tcgccgttga gcaccgccaa
      721 cgctcccagg cgcggattga tttgaatgaa ttcaacttcc aatccttcgt ccttaaaaaa
      781 tccccgcgcc tgggccacgt aatagctggt attggtgttg cttcggctcg acagcgagat
      841 acggatcttc tgcgtctgcg cctgaaccgc cggttgcggc gagcataacg ccaccacgcc
      901 cgctagcacc gctagtcccg accgccaatc tccgagaatc atttaatctc cttagttggc
      961 gcggattacc cgcgcattgt gaaatcgacc acttcgttgg gcgccacatt tgaagacaga
     1021 agtttgggcg gcttgcaatg taattgcacg actcagagat aacagccttt tgagtaaata
     1081 ggctagagca tagacccgcg tcaaggcgta attcgctctt tacaagccag ggcgattccc
     1141 attagagtca gcgccgtgaa gttgaaacta ggaattatcg tactcctatt cagctcgttg
     1201 ctgcggctta acgcgctggc caatccctat ctacccaagt caggcgagaa accatttcca
     1261 ctgcgcgtcg cgacttcagc ggtgaccggc ggttttatcc atctctatgc ggcgctggat
     1321 tacggcattc tcgaaaagta tggcttcaag cccgaacaca tctacatccg cggccagtcc
     1381 cctgcgctcg ctgctctagc cgccgatcaa actcagttca actacggcgc tgccgacggc
     1441 agcctccccg gcctcgccgc tggagtcgat gcaaaattcg tcgcctcgcc gctagtcaaa
     1501 ctcccctacg tcatggtggc gcgaaaagac attcgccggc cggaggattt gaaaggcaaa
     1561 tccatcggtg tcacccggcc tggcgatctg agcgcacggt tatcgcgtca agtgattcgc
     1621 aagtttgggt taactaccga cgacgtcacc attcatccca tcggcggcag ccagcaggaa
     1681 cgctatcagg caatggtcgg aaacgtcgta caggcgattc tggtcacgcc gccgctcgac
     1741 gtccgcgcca agagcgacgg ctttaacgtg atttatcggt tgatagatct ggaaattcct
     1801 tttgtctaca gttcgctcat gaccaattac aaaatgctcc gcgaacggcc ggagaccgtg
     1861 cagcgcatgg tcgcggcgct cgccgaagcg gtctatttcg tcgagaaaaa tcctgacaaa
     1921 gctaaggccg cggtagccaa agcgatgcgg gtgaaagacg aagaagcgct gcaaacctcc
     1981 tacaatgtgt acgcccgaga cattgtcgac cgcaccatgg tcgtgccggg taaatcgatc
     2041 accgaagcgt gggagctcgc ccgcgacacc gggactatcg tgagaagaaa gccggaagaa
     2101 ctctatgata acagcttcgt caccaatttg gagaagagcg gcttcttgaa agaactgtgg
     2161 ggcaacgaga attaccggaa gtgaatggca ctcgatccga cgtcgccgat tcaagaggag
     2221 cgattttcga tgaagattaa attattcgca ctcatcgtca tcttgctgtc ctcagcgccc
     2281 gccggcgcca atccgtatct ggccaaaccc ggcgagcgaa ccctgtcgat gcgcgtgtct
     2341 acctgcgcgg tgtcgggcgg gttcattcat ctgtataccg ccctcgacaa cggcctgttc
     2401 gataagtacg gcgtcaagct ggagaatatc tttatccgcg gcagcgcgca aaatctgact
     2461 gccctcgcca ccgatgaagt gcagttcact tactgcgctt cggacgcgat catccccggc
     2521 atggcagccg gcctcgaggt aaaaattgtc gcatcaccgc tggtgaagct gccatatgtt
     2581 ttagtcaccc gaaaagatgt gagacggccg gaggacctca agggcaaagc gctggggatc
     2641 gcccgcgccg gcgatttgag cgaccggttg tcgcgcgcgg tggtgaaaaa gctcggcctg
     2701 tctcccgaag cggtgacgat ccggcccatt ggcggcagcc agggtgaacg ttatcaggcc
     2761 atggccgccg gcattgtcca cggcgtgatc atcacgccgc cgctcgacgt tcgggccaag
     2821 aacgaaggct ataatgtaat gtatcgcctg atcgacctga atattccgtt tatctacagc
     2881 tcgctccaca ccagctacaa aatgctgcgc gaacgtcctg aaatcgtgca acgcctggtc
     2941 gccgcattcg ctgagatcat tcattttgtc gagaaaaatc ccgacaaagc gaaggcagcg
     3001 gtcagcaagg cgatgcgcac caacgacgcg gaagcgttgc agtctgccta cgacacctac
     3061 gcccgggaaa tcctcgaccg cacgatggtg gttcccggaa aggccgttgc tgaaaccatc
     3121 gagatcgccc gcgaaagcgg cagcccggtg cgaaagaagc cggaagaaat ctacgacaat
     3181 agcttcgtca acaatttaga gaaaagcggc tttatgaaag agatctgggg gaacgagaac
     3241 tatcggcgct agccggtgac ctgcatatcc gaacggattg aacggagcac agcggttgaa
     3301 ccgcttaaac tttttgaacg gctttccgtc tatttcacct gcaccttgcg caaccaactc
     3361 atgtcttcca gatcgtcggg cgtaaatgct ttggccacct taagcgactc gcggtagatt
     3421 tctatttcct tttccacggc ggcccgcggt ttggtgccgt ctttgctgta gcccaacatc
     3481 tgcgccgcca tcgctttctc ggccagtccc agatcgagct tgtgataagt cgctacatat
     3541 tgcacgactt cgttgcgatt ggctggattg agaatgtaat tcatcgtccg gtgcagcgcg
     3601 ctgaccatgc tttgcacctt ttgcgggctc ttcttcatgg tttccatgga gctcaccaag
     3661 ccgataaagc tcaacggggc gtaggttcgc gcttccgcga tcttcacgta gcccttggct
     3721 tccagctcct caacgtacgg cggcgccagc aacgttgcgg caattcgctt ggacgtgagc
     3781 gacttgatcg cgttgtcggt gacctgcatt gccaatagcg tcacccggcc ggcctcgatg
     3841 ccgtgggcgc gcaaaatcac gttggccgac atgccgaccg cgtccgtgag cgcagtgact
     3901 ccgatggctt tgccgaccaa gtcttgcaca gatttgatct ccggctgcga tatcagcaca
     3961 tgaaccgggt cttgctgcac gtagaacacc cgccgcagcg gcaacccgcg catgatcgcc
     4021 gaggtcgccg caccggtggt ggaagaaaaa tccaactcgc ccgctaccat cgcggcaatc
     4081 gccgtcggcg gcgccatcat gatcaactgc ggctcgatct gctcggcggc aaagaaaccc
     4141 ttatccttgc ccaagtagta cggcatgatc gacaagctct tggacggcac atgaattgtg
     4201 accctggtcg gggcggaagc gccgtgtgcc gctggcatag ccaacaataa tccgaaaacg
     4261 atcgctgcgt tacgaagtaa actgtttatg tccattgatt cacgaaacct ctcacgttga
     4321 tcgagacaat tcgattcacc accaagtgtt ggttagctta ggttcgtaaa cgaagtagga
     4381 aatcgcaacg aacgccgctc gacttggttt tccttactcg tcatgccggc gcaggccggc
     4441 atccaggcga ggaggggatg gataccggcg ttcgccggta tgacggaacc tcggtccatt
     4501 tcatcgccaa gatcttccta cacaatgaat
//
LOCUS       DATLCH010000366         3979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_7446829, whole genome shotgun sequence.
ACCESSION   DATLCH010000366 DATLCH010000000
VERSION     DATLCH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7446829"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1837)
                     /locus_tag="VMZ02_10960"
     CDS             complement(248..1837)
                     /locus_tag="VMZ02_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72499.1"
                     /translation="MLSLLAGPALSALAMEAQLGGPLPQPLPLFPANNWWNLDISNWP
                     VDQNSSSYISFINNGGTRRLHPDFGGNSGTGNGIYGMPVAVLTNVSNTDLKPVQFQYW
                     DESDGVDKSTGVGFPFYPIPNEAITQPRWIEGGDPGNIDKRTSEDRHLLLVDRDRNYL
                     YELYNVFFNTSQNKWYAGSGAFFDMNTNNRRPDTWTSADAAGLAILPGLIRYDETYDL
                     TATEIKHAFRVTVRATNGFVYPASHRAGSTSGALPMGARLRLKNTVDVTQKTSDPNVQ
                     KIFRAMQRYGLIVADNGSDMYITGTYDTRWNNDVLNPAFASLSASDFEVIQLGYNPAP
                     TAAALSSVTVTPSSATGGTSRAGSVTLTSAAPAGGVVVSLASSVGAAQVPGSITIPAN
                     SSSANFTVTTSSVSATTAGNISANYNGVTKSASISVLPPASAVLAALTLNPTIVAGGA
                     SSIGTVTLSAAAPSGGIVVNLSSNHPLRASVPATVTILSGSSSAKFTIATTKGSRKIV
                     ATISASQGGVTKSATLTVNRR"
     gene            complement(2078..3235)
                     /locus_tag="VMZ02_10965"
     CDS             complement(2078..3235)
                     /locus_tag="VMZ02_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HUR72500.1"
                     /translation="MKIIDVKTIRLRAEIPAAGQVFSRSGLRNTRSTTLIKVETDDGI
                     YGLGSASGNGELIEFIVARVLQPMLIGMDPTAIDEIWDAAYVRGGHKEFGTRGIGVVA
                     LSGVDIALWDILGKVHGVPLYQLLGGKVRDKVPVYATALYPEEPSKVARRARDFADQG
                     FHGVKIKVGFDLDQDIRIVRAVRQELGRDFIVMTDANQGYSIDVAINAAQAFADAGAF
                     WLEEPLFVEDIEGHAILRQRGNTPIAVGENLHMAYAFENFILRGAVDFIQPDVARAGG
                     ITEIRKITALAARHHVPVSFHTWGDGVALAASVHLCAAVQDCIVMELDYTYNPLREEL
                     LGEPFKVEKGYLTPSDKPGLGIELNPEALQKFAFAGTEDMAMRQKTLRLVR"
     gene            complement(3340..3777)
                     /locus_tag="VMZ02_10970"
     CDS             complement(3340..3777)
                     /locus_tag="VMZ02_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR72501.1"
                     /translation="MQQATTFKLKTNLLTGGRSHTPLAKSGGLTMGLNFYSPGRKNKL
                     HTHPGEDHAFVVIEGQATFYDKDNQPTVLDKGEGIMLPDGYYYYFESTGTVPLAILRS
                     SAARKDRPAVLRVDVKGDKRMAEEEGFAVVDGSAIEGQYWEMK"
BASE COUNT          844 a         1083 c         1121 g          931 t
ORIGIN      
        1 tctctcacgc cgttcgagat gacagatcag agaacgctga gttcacgccg tctggcgttg
       61 acaaaaaaat agattatacc gcgcgggaag gcgaggcagc aaatttacgt taaactgaag
      121 aatcatgagg gcgatttcga ctctgcgtcg ggttgacgca ctgcatgcga cggtttcctc
      181 agagatggcg gcgcggatcg tcgtttgcgc attcgggtga atcccatgta cgcgagagct
      241 atctcgctca acgtcggtta accgttagag tcgcgctttt agtgacgccg ccttgggatg
      301 cgctgattgt cgcaacgatt ttccggcttc ctttagtcgt tgctatagtg aacttggccg
      361 atgaactccc ggaaagaatt gtcactgtgg caggcacgct cgcgcgtaac ggatggttac
      421 tcgataagtt cacaacaatt ccgcccgatg gcgcggcggc gctgagcgtc acggttccga
      481 ttgaagaagc gccaccggcg acgatggtgg ggttgagcgt caaagcagcc aggactgccg
      541 acgcgggcgg caatacactg atcgaagccg actttgttac tccattataa ttcgctgaaa
      601 tatttccggc agtcgtcgcg ctgacggacg aagtggttac cgtgaagttt gccgagcttg
      661 aatttgccgg gatcgtgatc gagcccggca cctgtgcagc accgacgctg ctcgccaagc
      721 tgaccacgac gccacccgcc ggtgctgcgc tggttagcgt gacgcttcca gctctcgatg
      781 ttccgccggt ggcagacgaa ggcgtgacgg ttaccgagga aagtgccgcc gccgtggggg
      841 caggattgta accgagctgg atgacttcga aatcgctggc cgaaaggctt gcaaaagcag
      901 gattcaagac atcgttgttc caccgggtgt cgtaagttcc ggtgatatac atgtccgagc
      961 cgttatcggc gacgatcaag ccgtaacgct gcatcgcgcg aaatattttt tgcacgttag
     1021 gatcgctggt tttctgcgtc acatcgacgg tgtttttcaa acgcaaacga gcgcccatgg
     1081 gcaacgctcc gcttgtggag ccggcacgat gggaggcggg ataaacaaag ccgttggtcg
     1141 ccctcactgt tacgcgaaac gcatgtttga tttccgtcgc agtgaggtcg taggtctcgt
     1201 cgtagcgaat taatccagga agaatcgcca aacctgcggc atctgccgag gtccaggtgt
     1261 cgggacggcg attgttggtg ttcatgtcaa aaaacgcacc ggagccggca taccatttgt
     1321 tttgggacgt gttgaaaaag acgttgtaaa gctcgtagag gtagttgcgg tctcgatcga
     1381 ctaacaggag atggcgatcc tctgaagtgc gcttgtcgat atttcccggg tcaccgcctt
     1441 cgatccaacg tggctgtgta atcgcttcgt tgggtatcgg gtagaacgga aagccgactc
     1501 ccgtcgattt atcaaccccg tcgctttcat cccaatattg aaactgcacc ggcttaagat
     1561 cggtgttgct aacattggtt aaaaccgcga cgggcatacc gtagattccg ttgccggtgc
     1621 cggaattacc accgaagtcg ggatggagcc ggcgcgtgcc gccattgttg ataaaactaa
     1681 tatagctgga cgagttctga tccaccggcc agttgctgat gtcgagattc caccagttgt
     1741 tggctggaaa taacggcaga ggctgcggta gagggccgcc gagctgtgct tccatcgcta
     1801 gcgcggataa tgccggaccc gctaataaag atagcaccgt aaccgaggcg atgaatcgcc
     1861 gcagtcttaa attgtttacg cgaaatgatc gacaaagcat gcttgtctcc tcggctgtaa
     1921 cgacagtccg agcaggggag cgcaagcgct atgccaccaa ttatccggcg taaattttca
     1981 gcggtgcgtc gggaagttcc tgggtgatct agggatggtc agaaattttc tttgcgacag
     2041 tacagaaccg tgcattattt ttttgccacg aattaaatta tcggactaag cgcaaagttt
     2101 tttgccgcat tgccatatcc tcggtgccgg caaaggcaaa tttttgcaaa gcctcgggat
     2161 tcaattcaat gcctaatccc ggtttatccg agggtgtcag ataacccttt tcgactttga
     2221 agggctcgcc cagcagttcc tcgcgcagcg ggttgtaggt gtagtcgagc tccatgacaa
     2281 tgcagtcctg cacagctgcg cagaggtgca cgctcgccgc cagcgcaacg ccatcgcccc
     2341 aggtgtgaaa cgacaccggc acatgatgcc tggccgcgag cgcggtgatt ttgcgaatct
     2401 cggtaatccc gccggcccga gccacgtccg gctgaatgaa gtccactgcg ccgcgcagga
     2461 tgaaattttc aaaggcgtaa gccatgtgca ggttttcccc cacggcaatg ggagtattgc
     2521 cgcgttggcg cagaattgca tgcccctcga tgtcctcgac gaaaagcggc tcttccaacc
     2581 aaaatgcacc ggcatcggcg aaggcttggg cggcgttgat cgcgacgtcg atgctgtagc
     2641 cttgattggc atcggtcatg acgatgaagt ccctcccgag ttcctggcgc accgcgcgga
     2701 cgatacgaat atcttgatca agatcgaagc cgaccttgat tttaacgccg tgaaaacctt
     2761 gatcggcgaa atcgcgcgct ctgcgcgcga ccttcgacgg ttcttccgga taaagcgcgg
     2821 tggcgtagac cggaactttg tcgcgcactt tgccgccgag cagctgataa agcggcacgc
     2881 cgtgcacctt gccgaggatg tcccacaaag cgatgtcgac accgctcagc gcgacgacgc
     2941 caataccgcg ggtgccaaat tctttgtgac cgccgcggac gtacgcggca tcccaaatct
     3001 catcgatggc agtgggatcc ataccgatca acatcggttg caagactcgc gcgacgatga
     3061 attcgattaa ctcgccattc ccggaagccg aaccgagtcc gtatataccg tcatcggttt
     3121 cgactttgat cagggtcgtc gatcgtgtat tgcggaggcc cgagcggctg aacacctgtc
     3181 ccgcagccgg tatttccgcg cgcagccgaa tggtttttac gtctatgatt ttcatttctc
     3241 acgggaactt cataattcgt ggtcctgacg attatggttt ttcagcaatc aaacctgacg
     3301 attgatcatc catgccttgg tacggatctg tgatgatcgc tatttcattt cccagtactg
     3361 tccttcgatg gcgctgccat cgaccacggc aaatccttct tcttcggcca tgcgcttgtc
     3421 gcctttgacg tcgacgcgca acaccgccgg acggtctttg cgcgccgcgc tcgagcgcag
     3481 tatcgccaac ggtaccgtgc cggtgctttc gaagtaatag tagtagccgt ccggcaacat
     3541 gatgccttcg cccttgtcga gcacggtcgg ttggttgtcc ttgtcgtaaa aagttgcctg
     3601 cccttcgatc accacaaagg cgtgatcctc accgggatgg gtgtgcagct tgttcttccg
     3661 acccggcgaa taaaagttga gtcccatggt caggccgccg gatttggcaa gcggcgtgtg
     3721 gctgcgcccg ccggtgagaa gattggtttt cagtttgaac gtcgtagctt gctgcatacc
     3781 gctaattctc cttggctaat ttaatcagag tcgttatgtc agttcccaga attgccccgg
     3841 gatcgcctcg ccatccaccg taatgtactt atcttccact gcggttctct tgttgccttc
     3901 agcatccaca cgggtaaatt ccgggttgcc cttgagcgcg ctcacacgca gcagcgccaa
     3961 gggcttatcg ccggaattt
//
LOCUS       DATLCH010000367         6569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8000943, whole genome shotgun sequence.
ACCESSION   DATLCH010000367 DATLCH010000000
VERSION     DATLCH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8000943"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VMZ02_10975"
     CDS             <1..372
                     /locus_tag="VMZ02_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72502.1"
                     /translation="YLRNIVAVSEKTLRDDSRTVSRFVAGLAEGMQYYRNKSNKEDSL
                     KILAKYLRLPFEKNRALIEEGYETYREMMLRKPYADVNAMKILVDIIAESNPKAKSVN
                     LASLVDSSFVERLDREGMFDR"
     gene            complement(400..1302)
                     /locus_tag="VMZ02_10980"
     CDS             complement(400..1302)
                     /locus_tag="VMZ02_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72503.1"
                     /translation="MRATENVLSIVRNQPENTDEPAAPSDVPNLSFDDATGLVEIETQ
                     ANQLLDYISKLSSLADRACVTAIRQTETAQRSEESRQTEVATLRNQLEQSTVTLQERN
                     LAFATLESSTRSQVLSLEQRLRITERQLGEREGEISSLNRQISELAHRPMTPGYTEQQ
                     LEHEVAKKTAANEQEIAVLKNQLSQRDGLVQAKANMFKKAEADFRATIIDLETRLKEA
                     ESKLQLQETQLKDKEVVIQATAAKEVEIGKLIKRLSQECENLSNELQEKTRLMGQLEI
                     KKPQTQPDKNVWRRMIGRLQDDPL"
     gene            1967..3049
                     /locus_tag="VMZ02_10985"
     CDS             1967..3049
                     /locus_tag="VMZ02_10985"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HUR72504.1"
                     /translation="MAGNKLKGLVLSGGRGTRLRPLTHTAAKQLVPVANRPILFHVLD
                     NLQKAAVNDVGIVISPETGQAICDAVGDGRQWGMKISFIEQAEPLGLAHAVKVARPYL
                     GSDSFVMYLGDNLIGSGIESFRSDFEKSGALASILLKEVDNPSSFGIAEVDDQQRVLS
                     LIEKPREPKSKLALVGIYFFTAKIHEAIDKITPSWRGELEITDAIQLLLSQGGKVLSH
                     RIDAWWLDTGKKDDLLTANTVVLDEWVQRKIDGEIDGASQVTGRVQLGKGSRVVNSTV
                     RGPAVIGEDVLVENSFVGPFTSIGNGCRIVSSVLEHCLLLEAARVEFVDRLEDSLLGK
                     NSAVVREQKSRQAYRLMIGDDSEVLL"
     gene            3049..3612
                     /gene="rfbC"
                     /locus_tag="VMZ02_10990"
     CDS             3049..3612
                     /gene="rfbC"
                     /locus_tag="VMZ02_10990"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HUR72505.1"
                     /translation="MPFTFSSTKIADVIVIEPRVFPDERGFFMETYKRSEFAAHGISA
                     PFIQCNQSRSARGILRGLHFQKLPKAQGKLVRAVNGAIFDVAVDLRSGEPTYGQWVGM
                     ELSAANNKMLYTPPGFAHGFCVVSAEADIAYMASEEYAPTLESGIAWDDPDLNIDWPV
                     SAPQLSERDRKWPRLRDAEPGFGHAIV"
     gene            3637..4683
                     /gene="rfbB"
                     /locus_tag="VMZ02_10995"
     CDS             3637..4683
                     /gene="rfbB"
                     /locus_tag="VMZ02_10995"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HUR72506.1"
                     /translation="MKTVFVTGGCGFIGSNFVRLLATSTDYRIVNFDKLTYAGNLENV
                     ADISPARMRFVHGDICDRDLVERTLAEEKPWAVVNFAAESHVDRSILDSTPFLHTNIG
                     GVQSLLEAVRRVSVERFLQISTDEVYGDKAGKLPSTEDSPLAPSSPYSASKAAADLLC
                     FSYRRTYGLPILLTRSSNNYGSYQFPEKLIPLLLRNGLGDLDFPVYGDGQQIRDWIHV
                     EDNCLGLLAVLEKGEIGSIYNIGTSEERSNLAVVEAICAAIAAYKSVDLRQLTERIRY
                     VTDRPGHDRRYALDTSKVRKTLGWAPRVDFPGGLQRTVHWYLDNAHWVARVTSGEYRQ
                     YYESVYAQSWGKKL"
     gene            4684..5559
                     /gene="rfbD"
                     /locus_tag="VMZ02_11000"
     CDS             4684..5559
                     /gene="rfbD"
                     /locus_tag="VMZ02_11000"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HUR72507.1"
                     /translation="MDCVVVVGSSGQLGHDLVEVLHATDRYEVVALDHRRIECTDLNS
                     VRAALLPLRAQVIINCAAFVRVDDCEAQAREAFAVNALGALHVARAASESGAKCVYIS
                     TDYVFDGSKDSPYIECDIAAPINVYGASKLAGEHLVRQAAPHGLIVRVASLFGKTGAR
                     GKGGNFIETILAKAREGEPLKVVNDIVVSPTYAGDASRAIAQLIAVDAQGIVHAVNSG
                     TCTWYEFAETALKLCQFSAPIEAVSSLQFATPARRAKNSALVSARLVSEFSITMPHWR
                     DALRAYLIEKGHLPK"
     gene            5645..6406
                     /locus_tag="VMZ02_11005"
     CDS             5645..6406
                     /locus_tag="VMZ02_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR72508.1"
                     /translation="MRIIEKLFTTKQHGVAGYLAMPERSQPGPALYLIHPKGGMGEYI
                     KTETRKFAQLGYTTFAVNAFEQLGFPEETHIVTGAQIQAKTPDPEFTRVLTEGWRFLL
                     KQPNVNKQRVAVCGYCMGGRIAIHFVASVPEEVRAFVGYYPTVRDEPASALRPIHPSA
                     AVKKFRCPSVNLYGADDVTTVLPMQEAMYQAFKENGQPLEWHFFPFGGHGFVDPGAPG
                     YNPRTADLSWPLVADFLERNLVWSSPEVPNPNVIV"
BASE COUNT         1387 a         1715 c         1872 g         1595 t
ORIGIN      
        1 tatctgcgca acatcgtcgc agtgtccgag aaaaccctgc gcgacgatag caggacggtt
       61 tcgcgattcg tcgccgggct ggctgaaggc atgcagtact accgcaacaa gtcgaacaaa
      121 gaagacagcc tgaaaatcct agcgaagtat ctacgactgc cgttcgagaa aaaccgcgcg
      181 ctgatcgaag aggggtatga aacctatcgt gagatgatgc tgagaaaacc gtacgctgat
      241 gtcaacgcaa tgaagattct agtggatatc atcgccgagt cgaatccaaa ggcgaagtcg
      301 gtcaatttag cgagcctcgt ggactcttcg ttcgtcgagc gtttggaccg tgaggggatg
      361 ttcgatcgtt agtctatctt caccgcgctt ttgtaagtgt taaagcgggt cgtcctgcag
      421 ccggccgatc atgcgccgcc agacgttctt atccggctgt gtttgcggct ttttgatttc
      481 caattgaccc atcaaccggg tcttctcctg caactcattg ctcaggttct cgcattcctg
      541 agacagtcgt ttgatcagtt tgccgatttc cacttctttg gccgccgttg cttggatgac
      601 aacttcttta tctttgagtt gggtttcttg caattgtaat ttgctctccg cctctttcag
      661 tcgtgtttca agatcgatga tagtcgcgcg aaagtctgct tcagccttct tgaacatgtt
      721 ggccttcgct tgcaccaacc cgtcgcgttg actcaattga tttttgagaa cagcgatttc
      781 ttgctcgtta gctgcggtct tcttggcgac ttcatgttcg agttgctgct ccgtgtaacc
      841 aggagtcatg gggcggtgag ccaattcact tatctggcga ttcaacgagc tgatctcgcc
      901 ttcgcgttct ccgagttggc gctccgtgat gcgcagccgc tgttccaggc tcagcacctg
      961 actgcgtgtg ctgctctcga gcgtggcgaa ggctaggttg cgttcctgca acgttacagt
     1021 gctttgctca agctgattgc gcagagtcgc gacttccgtt tgacggcttt cttcgctgcg
     1081 ctgggcggtt tcggtttggc gaatcgccgt gacgcaggcg cggtcggcga gactgctcaa
     1141 tttgctaatg tagtcgagca actgattggc ttgtgtctca atctcgacta acccggtggc
     1201 atcgtcgaag gaaagattgg gtacatcact tggcgcggct ggttcatcgg tattttccgg
     1261 ttgattgcga acgattgaca aaacgttttc cgtggcacgc attggaaaat tcctccgggc
     1321 agttgtaaga cgactatgct ggcgattttg agcaatagtt ataccatcct cggccgcttg
     1381 gatttgaagg cacaaataag tgaaaagcca ggcttttttt atcttttact acggtgcagt
     1441 ggccgttaac gtcgagactt ggacaattac gtccgtgctt cgcaaataaa ggcaattcag
     1501 tgaccaacaa atagctgagg gtggccgtcc gaagcaacac accggggagg tggttgtcta
     1561 catcagtgtg gctaaaacca ttcgattgtt tgttcacccg cgtttaagtt cagacgcgaa
     1621 atgttacaga gaaatcgcag aaacgccgag ccgatgacga accgcctgtg gcgatgcgtg
     1681 gatgtttgac aatcgtgaga attcttcccg attagtacga cgtgacaggt cgatatcatg
     1741 aggctacagg tattgcgcgg agagtctgcg tcagtgaaag gggcgcgcaa gatctctatt
     1801 agattttctg ctcgggctgg cgtcacgaag gtccgccaag aatagcggga gttgcccgac
     1861 gcatagatcg tttttgggtt gtgttgctat tggaaaaaat tcctagtgcg ggtaatctta
     1921 gtcgacaaag tttccgactt gtgaatactg atccgcttgc tactcgatgg ctggaaataa
     1981 attaaaagga ctcgtcctaa gtgggggtcg gggtacccgg ctgcgcccgc tcacgcatac
     2041 tgccgctaaa cagcttgtgc cggtagccaa ccggccaata ttgtttcatg tcctcgacaa
     2101 tctgcagaaa gcggccgtca atgatgtagg aatcgtgatc tcaccagaaa ccgggcaggc
     2161 gatttgcgat gcggtgggag atggtaggca atggggaatg aagatttcct tcatcgagca
     2221 agccgaaccg ctcgggttgg ctcacgccgt gaaagtggcg cggccgtatt tgggtagtga
     2281 ttctttcgtc atgtatcttg gcgataacct tatcggttcg ggcatcgaaa gctttcggag
     2341 cgacttcgag aaatccggcg cccttgcttc gatattactc aaagaggtcg ataatccgtc
     2401 gagttttggt attgccgagg tcgatgacca acagcgagtg ctgagcctga ttgaaaaacc
     2461 gcgagagccg aaatcgaagc tagcgttagt gggcatttat ttttttacag cgaaaattca
     2521 cgaggccatc gataagatca cgccgtcctg gcgcggcgag ttggaaatca ccgacgcgat
     2581 tcagcttttg cttagccaag gcggcaaagt gctgagccac cgcattgacg cttggtggct
     2641 tgacaccggc aagaaggacg atctgttgac ggccaatacc gtggtcctcg acgagtgggt
     2701 gcagcgcaaa attgacggcg agatcgatgg ggcgagtcag gtgactggac gtgtccaact
     2761 tggcaaaggc agccgtgtgg tcaacagcac cgtgcgcggt cctgccgtca tcggtgagga
     2821 tgtgctcgtg gagaactctt ttgtcggtcc gtttacctcc atcggcaatg gctgccgcat
     2881 cgtgtcgtcg gtgctggaac actgcttgct gttggaagct gcgcgtgtgg aattcgtcga
     2941 ccgccttgaa gatagcttac tcggcaaaaa ctcagccgtc gtgcgcgagc aaaagagccg
     3001 ccaggcttac cggctgatga ttggcgacga ctcggaagtc ttgctctaat gccttttact
     3061 ttttcctcga ctaaaattgc cgatgtaatc gtcatcgagc cacgggtatt tcccgacgag
     3121 cgcggtttct tcatggaaac ctacaagcgg tcggaatttg ccgcacacgg aattagtgcg
     3181 ccgttcatcc aatgcaatca gtcgcgctcc gcgcgtggca tattgcgcgg cctgcacttt
     3241 caaaagctgc ccaaagctca aggtaagctc gtgcgtgctg ttaatggcgc gatcttcgac
     3301 gtcgcggttg atctgcgcag cggcgagccg acctacggtc aatgggttgg catggaattg
     3361 agcgcagcaa acaacaaaat gctttatacg ccgccgggtt ttgcccatgg cttctgcgtc
     3421 gtcagtgccg aagcggatat cgcgtatatg gccagtgaag aatacgcgcc gacgctggag
     3481 tccggcatcg cctgggacga tccggacctc aacatcgatt ggccggtttc ggctccgcaa
     3541 ttatcggagc gcgaccgcaa gtggccgcgc ttgcgagacg ccgaaccagg gttcggccat
     3601 gcgattgttt aagctcagtt ggtttcatcc gaaattatga aaacggtatt tgtcaccggc
     3661 ggctgcggat ttatcggcag caattttgtg cgtttgctgg cgacctcgac agattatcgc
     3721 attgtcaatt ttgacaagct aacctacgcg ggcaatttgg agaacgtcgc cgatatcagt
     3781 ccggcgcgca tgcgctttgt gcacggcgat atttgcgatc gcgatttggt cgagcggacg
     3841 cttgccgagg agaaaccgtg ggcggtggtt aattttgccg ccgagtccca cgtcgatcgc
     3901 agcattctcg actcgacgcc atttctccac accaacattg gcggtgtgca gtcgctgctc
     3961 gaagccgtgc ggcgagtgag cgtcgaacgc tttctgcaga tcagcaccga tgaggtttat
     4021 ggcgacaagg caggtaagct tccatcgacg gaggattctc ctttggcgcc gagcagtcct
     4081 tattcggcga gcaaggcggc cgcggattta ctttgcttct cttatcggcg cacgtacgga
     4141 ttgccgatcc tgctgacgcg cagttcgaac aactacggtt cgtatcagtt ccccgagaaa
     4201 ctcattccgc tgcttttgcg caacggcctg ggtgatcttg actttccggt ctacggcgac
     4261 ggccaacaga ttcgcgattg gattcacgtc gaggacaatt gcctcgggtt gttggcggta
     4321 ttggagaagg gcgagatcgg ctcgatttac aatatcggca ccagcgaaga gcgcagtaat
     4381 ctggcagtcg ttgaggccat ctgtgccgcc atcgccgcgt ataagagcgt cgatttgcgc
     4441 cagctcacag agcgtattcg ctacgtgacc gatcgcccgg gacatgaccg gcgctacgct
     4501 ctcgacactt ccaaagtacg taaaacgctt ggatgggcgc cgcgtgtcga tttcccgggt
     4561 ggtttacaaa ggactgtcca ttggtacttg gacaatgccc actgggtggc gcgcgtgact
     4621 tccggagagt atcgccaata ctacgaatct gtttacgcac aaagctgggg caaaaaactc
     4681 taaatggatt gcgtcgtcgt cgtcggcagt agcggccagc taggccatga tctggtcgag
     4741 gttctgcacg cgaccgaccg ctatgaagta gttgcactcg atcatcgtcg gatcgaatgt
     4801 accgacttga attccgtgcg tgcggcattg ctgcctctgc gcgcgcaagt gattatcaat
     4861 tgtgcggcat tcgtccgagt cgatgactgc gaggcacagg cgcgtgaagc gtttgccgtc
     4921 aacgcgctgg gcgcgcttca cgtcgcccgc gccgcgtccg agagcggcgc caagtgtgtt
     4981 tacatcagca ccgactatgt gtttgacggc agtaaagatt caccctacat cgagtgcgat
     5041 atagcggcgc cgatcaatgt ttatggcgcg tcgaagctcg cgggcgagca tttagtccgg
     5101 caagcagcgc cacacgggtt gatcgtccgg gtggctagtt tattcggtaa aactggcgcg
     5161 cggggcaagg gcggcaactt tatcgaaacg attctcgcca aagcgcgtga aggagagccg
     5221 ctaaaagtcg tcaacgatat tgtcgtttca ccgacctacg ccggcgacgc atcgcgggcg
     5281 atcgcccagc ttatcgcggt cgatgcccaa ggaatagttc acgctgtcaa tagcggcact
     5341 tgtacttggt atgagttcgc cgagaccgct ttaaaacttt gccagtttag cgccccgatt
     5401 gaagcagtgt catcgttgca gttcgccacg ccggcgcgcc gagcgaaaaa ttcggcgctt
     5461 gtgagtgcga gactggtctc cgaattttct attaccatgc ctcattggcg cgacgctctg
     5521 cgcgcctatc tgatcgaaaa aggtcatctg ccgaagtaac cgacctctct ctcgcaagcc
     5581 tttgcgccgc ggctggggat cgtgttagta acctggataa atatagtacc gcgaggaaat
     5641 cagcatgcgc ataatcgaga aattatttac cactaagcag catggcgttg ccggctacct
     5701 ggccatgccg gaacgcagtc aaccgggacc ggcgttgtac ttgatccatc ccaaaggcgg
     5761 tatgggcgag tacatcaaga ccgagacgcg gaagttcgct cagctgggct acacgacttt
     5821 tgccgtcaac gcattcgagc aattgggctt tcctgaagag actcatatcg tcaccggcgc
     5881 tcagatccag gccaagactc ccgacccgga attcactcga gtgctcaccg agggttggcg
     5941 cttcctgctc aagcagccca acgtcaacaa acagcgcgtg gctgtttgcg gctattgcat
     6001 gggcggtcgt atcgcgattc actttgtcgc gtcagttcca gaagaggtgc gcgccttcgt
     6061 cggttattat cccaccgtga gagacgagcc ggcctcagcg ttacggccga tccaccccag
     6121 tgccgcggta aaaaaatttc gttgcccgtc ggtcaattta tacggcgcgg acgacgtgac
     6181 tacagtgctg ccgatgcaag aggcgatgta tcaagccttc aaagaaaatg gccaaccttt
     6241 ggagtggcac ttttttccct ttggtggaca tggcttcgtc gatcccggcg cgcccggtta
     6301 caatccgcgc acagccgatt tgtcttggcc gctggtggcc gacttccttg agcgtaattt
     6361 agtttggagc tcgcccgagg tgccgaatcc gaacgtcatc gtgtaaaaat tgccaccgtc
     6421 cttgaaacga aaacgccgcc ctgagtgagc tcccagggcg gcgttttttg gacggtggtt
     6481 agtgattttg cgcgttcgct ttatcccccg cctcgcaaag tacccttgaa ctggtcgacg
     6541 gtcattttgc cggcgaccat aagttccat
//
LOCUS       DATLCH010000368        10471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10251013, whole genome shotgun sequence.
ACCESSION   DATLCH010000368 DATLCH010000000
VERSION     DATLCH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10251013"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VMZ02_11010"
     CDS             <1..1254
                     /locus_tag="VMZ02_11010"
                     /inference="COORDINATES: protein motif:HMM:NF018032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HUR72509.1"
                     /translation="VAGLTPEDIDTGAIIVTGEAVKKKNAEAIAALFSAEAGKFICAT
                     AGHNLESILAAYGSGAVHMTYHEGGEFTVMNVDVGGGTSKIAIVKEGRVIDTCAVEVG
                     ARLIAMDESGVITRLEDTALKFAKMAGIEGLQLGGTMSEEQKDKFSQKLADSLFEVLE
                     RGKLSAEVENLLLTPNIEFTDPIHAVMFSGGVSEFIYGYEKRNLGDLGAYLGKKLRAK
                     ANQLAGGKIPLRPAEVRIRATVIGASQYTVQVSGNTIYLSHPELLPLRNLQVVTPQFE
                     QNESINVSEVKEAVVRALQRFDADDIDRSVALAIRWELGPSYPLIRTLAEGLVQAMKG
                     HIDNGQPLVLVFDADIAKLMGNIIERELIPGAGIISIDGIDLKDFDFVDIGQELPDAK
                     AVPVVIKSLIFRNTEHGRGHHSQHQ"
     gene            1437..1622
                     /locus_tag="VMZ02_11015"
     CDS             1437..1622
                     /locus_tag="VMZ02_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72510.1"
                     /translation="MEEASGLHNFLEIVTKPDNIPIVAMLILVIFFTWLGMKEAFKND
                     KLIEEGREDEIPNEMWK"
     gene            1623..2456
                     /locus_tag="VMZ02_11020"
     CDS             1623..2456
                     /locus_tag="VMZ02_11020"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR72511.1"
                     /translation="MAYKWLAVLTAALAIATSTPFSLAAEFSAPFANDKRWEQARKDL
                     AQGKAAEAKAAFEELLRQYPDHADLHVFLGMSLLRLRDPQGAILSIKRAIAIDGDHVD
                     ARTLLGYVELEVRGDVAAATKEYRKVIELRPNSPEAHANLAVALKKQGDLDAAVASLN
                     KALEIKPDNVGALTTRGSIFAELGKWSEARRDFEQALKIDPRDDGALYGLSQSLREGK
                     EYAGAQSALSELISRSGNFVYWLEWGRIVMIRYWWALLLVAIGWALKGKLMKARTQAN
                     G"
     gene            2449..2994
                     /locus_tag="VMZ02_11025"
     CDS             2449..2994
                     /locus_tag="VMZ02_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72512.1"
                     /translation="MAEAKKRKGTALLAVMNENCSSCAGSPICEAHCPVDDCINLLYE
                     ELPGGGLKPYRVFVDNDKCIGCQMCYSDDLTKIQQHKETGEIFYEYANRFYDANRKPL
                     APDAVPKRIQLQLIGTDSEDRLDKKICPWDAIKMYEFDDGLAKSEYFYDQSKIKQING
                     VYVIDGAEKNRLEDKQSELYD"
     gene            3021..4394
                     /locus_tag="VMZ02_11030"
     CDS             3021..4394
                     /locus_tag="VMZ02_11030"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HUR72513.1"
                     /translation="MAGKIEIMETVLRDAHQCLVATRMPTEDMLPIAAALDDVGYWSL
                     ETWGGATFDATLRYLKECPWERLRKLRAAMPKTRFQMLLRGQNVVGYKNYPDDIVAAF
                     VQRAAANGIDVFRIFDAMNDVRNMKTAIEAGLKTGKLVEGTVCYTISPVHSPDYFLDV
                     AEKLADMGVQIICLKDMGGLLAPYAAYEIIKKIKSRVPLPLHLHSHCTAGLAQMTYMM
                     AIEAGADILDTAISPFSQGTSQPATEQIVAGLMGTPHDTGLDLAKLAKIAEYFYRIRP
                     RYREFESPVNNQIKPDVLVSQIPGGMLSNLIAQLRQQKAEDKLEAVLAEMPHVRKDLG
                     YPPLVTPTSQMVGAQAALNVMTGKRYSVVAMETRNYVMGLYGEAPGKIGDQIKEKVLG
                     KKEPITCRPADLLKPGLEKARSEAGSMAKSEEDVLTYALFPDIAKDFFLHRDSTEGRQ
                     QAAVASH"
     gene            complement(4452..5351)
                     /gene="hisG"
                     /locus_tag="VMZ02_11035"
     CDS             complement(4452..5351)
                     /gene="hisG"
                     /locus_tag="VMZ02_11035"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HUR72514.1"
                     /translation="MAGDKELKLGIPKGSLEGMTIELFKKSGWKIASSSRSYFPTIDD
                     AEIRCSLARPQEMSRYVEMGTLDVAITGKDWTIENNSNVEVVCEMVYSKTSFRPTRWV
                     VAVPRESPVKKLEDLKGKRISTEMVNFTKRYFAERNIPVEVEFSWGATEAKAAEGLVD
                     AIVEVTETGTTIRAAGLHIIHELMESCPQLIANKAAWQDPWKQAKIAQIKMLLLGALS
                     AENKVGIKLNVPQGNLGKVVELIPSITAPTVATLHPSPNLKGVKWYSVESVLEETKVR
                     DLIPKLIEAGAVGIIEYPLNKII"
     gene            complement(5367..5750)
                     /gene="hisI"
                     /locus_tag="VMZ02_11040"
     CDS             complement(5367..5750)
                     /gene="hisI"
                     /locus_tag="VMZ02_11040"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HUR72515.1"
                     /translation="MSDVRIDFAKGNGLVPVIVQDHESREVLMVAYMNEEAWQKTQET
                     GKAHYYSRSRKGPWFKGEESGNFQEVKEAYIDCDTDTILIQVHQVGGAACHEGYKSCF
                     FRQRTGAGWKVIGERVFNPKEVYKK"
     gene            6014..6823
                     /gene="cutA"
                     /locus_tag="VMZ02_11045"
     CDS             6014..6823
                     /gene="cutA"
                     /locus_tag="VMZ02_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HUR72516.1"
                     /translation="MSELIVVYVTVGTGEEADRMAQALVGEQLAACVNRVRSIQSVYR
                     WQGKIEQNDEELLIIKTRRQLFPALEKRVKELHSYSVPEIIGLPIIEGSSDYLTWLRE
                     QALAKPPQDENIRTVREVSAGGVIYRRSGELFEVALIHVRNRWGLPKGHVEEGERVEE
                     TALREVREETGLEGKLVRKLGDIRYSYRDKTKGDEPIRIYKRVHFYLMRYVRGDVRDH
                     DHESDDARWFPLDQAMKQLKFATERKMVHRAMTMLTARPAEPQTPAAERGA"
     gene            6903..8285
                     /locus_tag="VMZ02_11050"
     CDS             6903..8285
                     /locus_tag="VMZ02_11050"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HUR72517.1"
                     /translation="MAGDSIGVTFSVPYEMAVVRPLRQTTEQERQAALVSAHYNAELI
                     PQELIYIDLSTDSGVSARSTAQLAASLTAKAVEPGMGLASEGSGAYNRLAAGIQRYFG
                     FEYFVPTTQGRSAERIWMKVTVKPGSIVAGNMLFPSTRSHIEMSGAQIVDVISDAAHD
                     LTSNEPFKGNIDVQKLEALIEEKGAEQISCVYVELSVNACGGHPVSLANLKSVREITA
                     AHKIPLFSDASRILENSFLIKEREAEHRQRSLADIVRDICALFDGGTMSALKDCLVDS
                     GGFVFTRDRASHQKASMQSFLDGVQLSAGDMESITVALPEIFASEAHARQRFEQVKYL
                     WHQLADTLPVLRPAGGHAVFIDLKKFFAGSTPQPFAAEALAAYIYLKSGVRLTKGPPL
                     APSQVARGAELLRVAVPARKYLNGHMDDVANALRAAYAHRDEIRALKKIDDLTRSRYD
                     PGHFVIYELP"
     gene            8272..10047
                     /locus_tag="VMZ02_11055"
     CDS             8272..10047
                     /locus_tag="VMZ02_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HUR72518.1"
                     /translation="MSFPEIPASGLYVGQVFFQRAAELGDRTFIKFQKCERFEEISWR
                     DFAAIVSHTLLGLCALGITPGDTVAIIGENSLAWLAADLATLAGGFTNVVISPNLSNN
                     MFAKVLGHAGCRAAFVHDAETARRLLDLQEQLPALQHVLIMNDGGFGNAKALNFRDLM
                     ERGSRGRNAQRARLVEAVGRNDLATIMYTSGSTGEPKGVMRTQGNLLANITNGGAITV
                     SGPDELTVNVLSLNHLFGRFGFLKSAVTGRTTALVEAGETKLDLSVLAQLAPTALTVV
                     PRVMERIWDCVLAHDDTQELWRELENLDGRHYSGLNTEAELKRFEELKGQIQANVRRM
                     LGGRIKYISYGGAAMPPRIMRFFALIGIPLVGSYGSTECGGVTLCGLGETRPGNLGKP
                     FANVEIRIAADGEILVRGPTVSPGYFKNPEATAEAFDEEGWYRTGDLGLLETDGSLRI
                     IGRKKDVFNCADGSNIYPGFIELQLENEPFISEAVLIGDRRPFIAALIVPDRQRIAES
                     LDHDIAALSKSEITAAVWRQIERVNSRLEKYERICRIEIIPDKFPADIRSINHFHKVK
                     VNREQVGARFRPQIDAMYATTQTRG"
     gene            10050..>10471
                     /locus_tag="VMZ02_11060"
     CDS             10050..>10471
                     /locus_tag="VMZ02_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HUR72519.1"
                     /translation="MKQIALITGGGGGIGGAVAQRMAAAGITVVIADYNGAAAERVKG
                     EITAAGGTADAITIDVTVAREVKAVVADVAQRHGRIDILANIAGGSAYTKRIEEFTWA
                     EWQQVMEANLKGTFLMCREVAPIMQKQRSGRIINTASN"
BASE COUNT         2378 a         2762 c         3010 g         2321 t
ORIGIN      
        1 gtcgccggct tgactccaga ggatatcgat accggtgcga tcattgttac cggcgaagcg
       61 gtcaagaaga aaaatgccga agcgatcgcg gcgctgtttt ccgccgaagc cgggaaattc
      121 atctgtgcta cggcagggca taacttggaa tctattttag ctgcctacgg ctccggcgcg
      181 gtgcatatga cctatcacga aggcggcgaa ttcaccgtca tgaacgtaga tgtcggtggt
      241 ggcaccagta agatcgccat cgttaaagag ggccgggtga tcgatacctg cgccgtcgaa
      301 gtaggcgcgc ggctaatcgc catggatgaa agcggcgtca tcacccgcct cgaagacacg
      361 gccctaaaat ttgccaagat ggccggtatc gagggcctac aattaggcgg caccatgagc
      421 gaggagcaaa aggacaagtt ctcgcaaaaa ttggccgatt cgctgtttga ggtgctggaa
      481 cgcggtaagc tgtcagctga ggtggaaaat ctcctgctaa cgcccaacat tgagtttacc
      541 gatccaattc acgcggtcat gttctccggt ggcgtttccg agtttatcta cggctacgaa
      601 aagcgcaatt taggcgatct cggcgcatat ctaggaaaaa agttgcgcgc aaaggccaac
      661 caattggcgg gcggaaagat tcccttgagg cctgccgaag tgcggattcg cgccactgtc
      721 atcggcgcgt cgcagtacac ggtccaggtg agcggtaata ctatatacct gtcgcatccc
      781 gagcttcttc cattgcgcaa cctgcaagtg gtcactccgc agttcgaaca gaatgagtcc
      841 attaacgtga gcgaggttaa agaggcggtg gtgcgggcgc tacagcgctt tgacgccgac
      901 gatattgacc gctcggtggc attggcgatc cgctgggaat tggggccttc ttatcctttg
      961 attcgcacct tggccgaggg gctagtgcaa gcaatgaaag gtcacatcga taatggtcag
     1021 ccgctggttt tagtgttcga cgccgatatt gctaaactga tgggcaatat catcgagcgc
     1081 gaactgattc ccggcgcggg aattatttcc atcgacggca tcgatttaaa ggatttcgat
     1141 ttcgtcgata tcggccaaga gctgccggac gccaaggcgg tgccggtcgt gatcaagtcg
     1201 ctgatctttc gcaataccga acatggccgc gggcatcact cgcagcatca gtaatttctt
     1261 ggatctatcc ccgaggctag cagatcgcct tcgctcgctc gatcgtccgc aatggccgat
     1321 ccgctacgct cgagcttttt ctgccaacaa gtcacattga cttccggtgg aatttttaat
     1381 atttttctcg gtattaactc aaaagaaaat acttagaata cgagcggtta acttttatgg
     1441 aagaagccag cggattgcac aattttctcg aaatcgtcac taaacctgac aacatcccaa
     1501 ttgttgcgat gctgatcctg gtgatctttt ttacctggct tgggatgaag gaagccttca
     1561 agaacgacaa gctcatcgag gaaggtaggg aagacgagat tcccaacgaa atgtggaaat
     1621 aaatggcgta taagtggctg gcggtgctga ccgcggccct cgcaatcgct acatcgacac
     1681 ctttttctct cgccgccgaa ttctctgccc cttttgcaaa cgataaacgc tgggaacagg
     1741 cgcgtaagga tctcgcccag ggtaaggctg ccgaagctaa ggcggcattc gaggagcttc
     1801 tcaggcaata tcccgatcat gccgacctgc acgtcttctt aggcatgtcg ctgctccgac
     1861 tgcgcgatcc ccagggggcg attttatcga ttaagcgggc tatcgcaatc gacggcgatc
     1921 atgtcgatgc acgaacgctg ctgggctatg tcgaattgga agttcgcggc gatgtcgccg
     1981 cggcaaccaa agaataccgc aaggtcatcg agctgcggcc caactcgccg gaggctcatg
     2041 ccaatctggc ggtggcattg aaaaagcaag gcgatctcga cgccgctgtc gccagtttga
     2101 acaaagctct ggaaattaaa cctgataatg tcggtgcttt gacgaccaga gggagtattt
     2161 tcgccgagct cggcaaatgg agcgaagcac ggcgcgattt cgagcaggcg cttaagatcg
     2221 acccacgcga cgacggcgcg ctctacggac tgtcacagtc gttgcgcgaa gggaaggaat
     2281 atgccggcgc gcagagcgcg ttgagcgagt tgatctcccg ttcgggaaat tttgtttatt
     2341 ggctcgagtg gggccgcatt gtaatgatcc gttattggtg ggcgctcttg ctggtggcaa
     2401 tcggctgggc gctcaagggc aagcttatga aggcgaggac gcaagcgaat ggctgaagca
     2461 aaaaagcgca aaggtacggc tctattggcg gtcatgaacg aaaactgttc gagctgtgcc
     2521 ggctcgccca tctgcgaggc gcattgcccg gtggacgact gcatcaatct actctacgag
     2581 gagttgccgg gcgggggttt gaagccctac cgcgtgttcg tcgacaacga caagtgtatc
     2641 ggttgccaaa tgtgttatag cgacgatctg accaagatcc agcagcacaa agagaccggc
     2701 gagatttttt atgagtatgc caacagattt tacgacgcga accgcaagcc gctcgcaccc
     2761 gatgccgtac ccaaacgtat tcaactgcaa ctgatcggca cagactcgga agaccgcttg
     2821 gataaaaaaa tctgtccatg ggacgccatc aagatgtatg agttcgacga tggtttggca
     2881 aagtcggaat acttttacga ccagagcaag atcaagcaaa tcaatggcgt gtacgtcatc
     2941 gatggcgcgg aaaaaaaccg cttggaagac aaacaaagcg agctttacga ctaggccggc
     3001 aggataggga gccaacttat atggcgggta agatcgagat catggaaacc gttttgcgag
     3061 acgcacacca gtgtctggtc gcgacgcgca tgccgaccga agatatgctg ccaatcgccg
     3121 cggccctcga cgatgtcggc tactggtccc tcgaaacctg gggcggcgcg accttcgatg
     3181 cgaccttgcg ctacctaaag gaatgcccct gggagcgctt gcgcaagctg cgcgccgcca
     3241 tgcccaagac ccgatttcag atgctcctac gcgggcaaaa tgtcgttggc tacaaaaact
     3301 accccgacga tatcgtcgcg gcgttcgtgc agcgcgccgc ggccaatggc atcgacgtgt
     3361 ttcgcatttt cgacgccatg aacgatgtgc gcaacatgaa aaccgccatc gaagcaggtc
     3421 tcaagaccgg caaactggtc gagggcacgg tctgctatac catcagtccg gtacattcgc
     3481 cggattattt cctggacgtg gcggaaaagc ttgccgacat gggtgtgcag atcatttgcc
     3541 tgaaagatat gggcggatta ctggcgcctt acgctgccta cgagatcatt aagaaaatca
     3601 aatctcgcgt cccgctgccg ctgcatttgc attcacattg caccgcgggt ttggcgcaga
     3661 tgacctacat gatggcgatt gaggccggcg cggatattct ggataccgcg atttcgcctt
     3721 tttcccaagg cacatcgcag cccgccaccg agcagattgt tgccggcctt atgggcacgc
     3781 cgcatgatac cggactagat ctcgctaagc tggctaagat cgccgagtat ttttatcgca
     3841 ttcggccgcg ctatcgtgag ttcgagagcc cggtaaacaa tcaaatcaag ccggatgtct
     3901 tggtctcgca gattcccggt ggcatgctct cgaacctgat cgcgcaattg cgtcagcaaa
     3961 aggccgagga caaactcgaa gcggtgctcg ccgagatgcc ccatgtgcgc aaggatttgg
     4021 gttatccgcc gctcgtgaca ccgaccagcc aaatggtcgg tgcccaagcc gcgcttaacg
     4081 ttatgaccgg caaacgctac tccgtggtcg caatggaaac caggaactat gtcatgggac
     4141 tgtacggcga agcgccgggc aagatcggcg atcagattaa agaaaaagtg ctcggcaaaa
     4201 aggagccgat cacctgccgg ccggcggact tattgaagcc cgggcttgaa aaggcccgca
     4261 gcgaagccgg cagcatggcg aagagcgaag aagatgtgct cacctacgcg ctgtttcctg
     4321 acatcgccaa ggactttttt ctgcaccggg acagtactga aggaaggcag caggcagcgg
     4381 tcgcaagcca ttagcagtcg ctgcttgtcg caaacgggtt agctcgtctc gggcttcgtc
     4441 gacttcgatt cttagataat tttattcagc ggatattcga taattccgac agcgccggct
     4501 tcgatcagct tgggaataag atcgcgcact ttggtttctt cgagaacgct ctcgacggaa
     4561 taccacttga cgccctttag attgggcgat ggatggagcg tcgccaccgt aggcgcggtg
     4621 atacttggaa tcaactcaac gacttttccc agattgcctt gcggcacgtt gagttttatg
     4681 ccgactttgt tctcggcgga aagcgctccg agtaagagca tttttatctg cgcgatcttt
     4741 gcctgcttcc agggatcttg ccaggccgcc ttgttggcga tcaactgcgg acacgactcc
     4801 atcaattcgt gaataatatg taatccggcg gcgcggatgg tagtgccggt ctcggtaact
     4861 tccacgatcg catcgacgag tccctcggct gctttcgctt cagtggcgcc ccaggaaaat
     4921 tctacttcta cgggtatgtt gcgttcggca aaatagcgct tggtgaagtt caccatttcc
     4981 gtggaaatgc gcttgccttt gagatcttcg agttttttga ctggtgactc tcgcggcacc
     5041 gcgacaaccc agcgggtggg ccgaaagctg gtctttgaat agaccatctc gcaaacgacc
     5101 tcgacattgg agttattttc gatcgtccag tctttgccgg tgatggcgac atcgagtgta
     5161 cccatctcga cgtagcgcga catctcttgc gggcgcgcca aactgcagcg gatttccgcg
     5221 tcatcgatgg tcggaaagta gcttctgctg gatgacgcaa tcttccaacc cgattttttg
     5281 aagagctcga tggtcatgcc ttcgagactg cccttgggaa tacctaactt gagttctttg
     5341 tcgccggcca tggaaaagtc ttatctttat tttttgtaaa cttcttttgg attgaaaacg
     5401 cgctcaccga ttactttcca gccggcgccg gtgcgctggc ggaaaaaaca acttttgtag
     5461 ccctcgtggc aggcggcgcc gccaacttga tgtacttgaa tcaggatcgt gtcagtgtcg
     5521 cagtcgatat acgcctcctt aacttcttga aagttgccgg actcctcgcc tttgaaccac
     5581 gggcctttgc gcgagcggct gtagtaatgg gctttcccgg tttcctgtgt tttctgccac
     5641 gcttcttcgt tcatgtacgc gaccatcagt acctcacggc tctcatggtc ctgcacgata
     5701 accggcacca agccgttacc tttggcgaaa tcgattctca cgtctgacat gggattttct
     5761 ccgaacctgt ttagctaaca tagatatgtg ggtgcttcaa ccagaggacg gagtccggcc
     5821 gccggaggaa attgccgtat cagttgcctc taatacgcgg atttgctcac tttttgggag
     5881 ctgtctggca cttgctgatt accggaattc gaattggcag cggtggctag tgaatattcg
     5941 tgcgcttctc gcctattgcc attgttttcc gcagattgac tggatcacca agcgggttgt
     6001 aagattgcca gtgatgtcgg aattgatcgt agtttatgta acggtgggga ccggcgaaga
     6061 ggcggatcgc atggcccaag ccttggtcgg ggagcagttg gctgcctgcg ttaatcgcgt
     6121 gcgctcgatc cagtcggtgt accgctggca aggcaaaatc gagcagaatg acgaagaact
     6181 gttgatcatt aaaactcgtc ggcaactttt cccggctttg gagaagcgcg tcaaggaact
     6241 gcacagctat tccgttcccg agatcatcgg tctgccgatt atcgaaggca gctcggatta
     6301 tttaacttgg ctgcgcgagc aggccttggc gaagccgccg caggacgaaa atattcgcac
     6361 cgtcagagaa gtttccgccg gcggcgtgat ctaccggcgc agcggcgagt tgttcgaagt
     6421 agcactcatt catgtgcgca accgctgggg gctgccaaag gggcatgtcg aggaaggtga
     6481 acgggtcgag gaaacggcct tacgcgaggt gcgcgaggaa accggtctcg aaggcaaatt
     6541 ggtgcgcaag ctcggcgata ttcgctactc ctatcgtgac aagaccaaag gagacgagcc
     6601 gatccggatc tacaagcgcg tgcatttcta cttgatgcgc tatgtgcgcg gcgatgtgcg
     6661 cgatcacgat cacgagtcgg acgatgcccg ttggtttcct ctcgatcagg cgatgaagca
     6721 gctcaaattt gctactgagc gcaaaatggt gcaccgcgcc atgaccatgt tgaccgcgcg
     6781 cccggcagag ccgcaaactc cggccgccga acgcggtgct tgagttctaa actatttttc
     6841 aaagcttgcc gatttcgtgt tatagaatgg ccagcgattg actcttctgg aaaactcccg
     6901 cgatggccgg cgattcgatt ggtgtgacgt tttccgtgcc ttatgaaatg gcggtggtgc
     6961 ggccgctacg gcaaaccaca gagcaggaac ggcaagccgc gctcgttagt gctcactaca
     7021 acgccgagct gattccccag gaactcattt acatcgattt atcgaccgac agcggcgtaa
     7081 gtgcgcgtag cacggcgcag ctggcggcct cgttgacggc taaggcagtc gaacccggca
     7141 tgggtttggc gagcgaagga agtggtgctt acaatcggct cgccgcagga atccagcgct
     7201 atttcggatt cgagtacttc gtgccgacaa cccagggccg ttcggcggaa cgaatttgga
     7261 tgaaggtcac tgttaaaccg ggcagcattg tcgccggtaa catgctgttc ccttcgacgc
     7321 gcagccatat tgaaatgagc ggggcgcaga ttgttgacgt tattagtgac gcggctcatg
     7381 atcttacgtc gaacgagccg ttcaaaggca atatcgatgt gcaaaaactt gaggcgctaa
     7441 tcgaggagaa gggcgctgag cagatcagtt gcgtttacgt cgaattaagt gtcaatgcat
     7501 gcggcggtca tccggtgtcg ctggccaatc tcaagtcggt ccgggagatc acggcggcgc
     7561 acaagattcc gcttttttcc gacgccagtc gtattctcga aaacagcttt ctcatcaaag
     7621 agcgagaagc ggagcaccgg cagcgcagtc tggcagacat cgtgcgggat atttgcgcgc
     7681 tctttgacgg cggtaccatg agcgcgctca aagattgcct agtcgatagc ggcggatttg
     7741 tatttacccg cgaccgcgcc agccatcaaa aggcctcgat gcaaagtttt ctcgatggtg
     7801 tccagctttc ggctggcgac atggaatcca tcacagtagc gctgccggaa atcttcgcca
     7861 gcgaagcgca cgccagacaa cgattcgagc aagtgaaata tctatggcat caactcgcgg
     7921 acacattgcc ggtattgcgg cccgcgggtg ggcacgcggt atttatcgat ctaaaaaaat
     7981 tctttgccgg gagcacgccg cagccctttg ccgccgaagc cttggccgct tatatttatc
     8041 tgaagagcgg cgttcgcctc accaaggggc cgccgctggc gcccagtcaa gtggccaggg
     8101 gcgccgagct tttacgagtt gccgtgccgg cgcggaaata tttgaacggc catatggatg
     8161 atgtcgccaa tgcgctgcgc gcggcttacg cgcaccgcga cgaaattcga gcactaaaaa
     8221 aaattgacga tttgacgcgg tcgcgatacg atccggggca tttcgtcatt tatgagcttc
     8281 cctgaaattc ccgctagcgg cctttacgtc ggccaggtct ttttccagcg cgccgccgag
     8341 ttgggcgatc gcacgttcat caagttccaa aaatgcgaaa gattcgaaga gatctcgtgg
     8401 cgggactttg ccgccatcgt cagtcatacc ctgctcgggt tgtgtgcgct tggcattacg
     8461 cctggcgata ctgtcgcgat catcggcgag aatagcctgg cctggctggc tgccgacttg
     8521 gcgaccctcg cgggcggatt taccaacgta gtcatctcgc caaatctttc caataacatg
     8581 ttcgctaagg tgctgggtca tgccggctgc cgagccgcgt tcgttcacga tgctgaaacc
     8641 gctcgccggc tgctcgatct ccaggagcag ttgccggctt tgcagcatgt gttgatcatg
     8701 aacgatggcg gttttggcaa cgccaaagcg cttaattttc gcgacttgat ggaaagggga
     8761 agccgcggcc gaaatgcgca gcgtgccagg ctcgtcgaag cggttggtcg aaatgacttg
     8821 gcgaccatca tgtacacttc aggctcgacg ggagaaccca aaggagtaat gcggacgcaa
     8881 ggcaatctgc tggcaaatat caccaacggc ggtgccatca cggtcagcgg cccggacgaa
     8941 ttaacagtaa acgtgcttag cctgaatcac ctcttcgggc gcttcggctt tcttaaaagt
     9001 gcggtgaccg gacgcactac ggcgctcgtt gaggccggtg agacgaaatt ggacttgagc
     9061 gtattggcgc aactggcacc tactgctttg actgtggtgc cccgagtgat ggagcggatc
     9121 tgggattgtg tactggccca cgacgacact caggaattat ggcgcgaatt agaaaatctc
     9181 gacggcaggc actacagcgg ccttaataca gaggcggaat taaaacgttt cgaggagttg
     9241 aaaggtcaaa ttcaagcaaa tgtccgacgc atgctcggcg gccggattaa atacatatca
     9301 tacggcgggg ctgccatgcc gccgcgcatc atgcgctttt ttgcattgat cggcattccg
     9361 ttggtcggct cttacggctc gaccgagtgc ggtggcgtaa cactctgcgg tctcggcgag
     9421 actcggcccg gcaacctagg gaaaccgttc gcgaacgtcg aaattcgcat cgccgcagac
     9481 ggcgagatcc tggtgcgcgg tccgactgtg tcgccgggct atttcaaaaa tcccgaagct
     9541 accgccgagg cattcgacga agaaggctgg tatcgtacgg gtgatttagg attgctcgaa
     9601 accgatggct ccttgagaat catcggacgg aagaaagatg tcttcaactg cgccgacggt
     9661 tcgaacatct atccgggttt catcgaactt cagttagaaa acgagccatt tatcagcgag
     9721 gcggtcttaa tcggcgatcg ccggccgttt atcgcggcgc tgatagttcc agaccgccaa
     9781 aggattgccg aatcgctcga ccatgatata gcagctttgt cgaagagcga aatcaccgcc
     9841 gccgtgtggc ggcagatcga gcgcgtcaat agtcggctag agaagtacga acgaatttgc
     9901 cggatcgaaa ttatacccga taagtttccc gccgacatca ggagtatcaa ccactttcac
     9961 aaggtcaaag taaatcgcga acaggtcggg gcgcgctttc gtccccagat cgacgccatg
    10021 tacgcgacta cgcaaacgag aggatgacat tgaagcagat agcattgatc acgggcggcg
    10081 gcggtggcat cggcggcgcg gtggcgcagc gcatggctgc ggcagggatc accgttgtca
    10141 tcgctgatta caatggcgca gcggcagagc gggttaaggg cgagatcact gcggctggcg
    10201 gcacggccga cgctataacc atcgacgtga cggtggctcg cgaagtcaaa gcggtggtcg
    10261 ccgatgttgc tcagcgtcac ggccgcattg atattctagc caatatagcc ggcggcagcg
    10321 cctacaccaa acgcattgag gagttcactt gggcagagtg gcagcaagtg atggaggcca
    10381 atttaaaagg tacttttctg atgtgccgcg aagtggcgcc aatcatgcaa aaacagagaa
    10441 gcggccgaat catcaatacg gcgtcgaact a
//
LOCUS       DATLCH010000369         6035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8035829, whole genome shotgun sequence.
ACCESSION   DATLCH010000369 DATLCH010000000
VERSION     DATLCH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8035829"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VMZ02_11065"
     CDS             <1..556
                     /locus_tag="VMZ02_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72520.1"
                     /translation="IGFSDPRVPSSGQSIWSFMWELKGEEFLKKLVQQELFLTRDLRQ
                     LADALAKGKVAIAFGLGRSQTDPFVDAGLPIKPVPAPKEGLPASNSFGVIGVIKNPPN
                     PNATKVFVNWFLSREGQEWYGRIMENGTRRLDVETKWLNKIGVSAAKDTITIQEYHRL
                     RNHLEDKYTKVRVPAGRFAETILK"
     gene            617..1693
                     /locus_tag="VMZ02_11070"
     CDS             617..1693
                     /locus_tag="VMZ02_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72521.1"
                     /translation="MKNFINISIMALALVIGSAATCGAATVDEVMLYNKPDRQKILLE
                     GAKKEGKITWYTSLIIDQVVRPVKEAFEKKYPFIQIEPFRGNSERIVQKMFAEYQGKR
                     YEVDIMDGTVTAPMVKKGGYLQRFYSPHLAEYPAELKDPQGYWGVSNVYYFALGYNTR
                     MVKPGEVPKTYEDLLNPRWKGQMMWSTSRGSGAPMMIGNIFQSMGQEAGKAYLQKLKA
                     QNVAKSTASNRQLLDLVMAGEYPLALHIFHHHAYISRTAGAPVDWAPLEPLSATINTI
                     SPVTRSPHPHAAMLLLDFILSEEGQKVIQAANYLPSHPKVPAKQVDLKPGGGKFKRAL
                     YFTPDSQVNEGDSWVDYFEKNFLK"
     gene            1763..2863
                     /locus_tag="VMZ02_11075"
     CDS             1763..2863
                     /locus_tag="VMZ02_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72522.1"
                     /translation="MKFFRAGADSFRTLLRMASFLVVLACAQTALAASVEEIALLKSA
                     ERQRILVEGAKKEGKLSFYTTLIIDQVIRPMKEAFEKEYPFIQMEYFRGNSDRLAQKL
                     FAEYQAKRYAVDVVDGSSTTTMVRRAGFLQRFYSPHLAEYPAELKDAEGFWGVSNLYF
                     HTIGYNTRMVKPNDVPKTYEDLLHPRWKGQMIWSTGRGGGGPLFVGNVLQSMGENAGK
                     AYLQKLRGQNIAKSTASSRQVLDMVIAGEQPIVLVIFNHHAYISKNAGAPSEWQPLEP
                     VPARVSTIGLPKFAPHPHAAMLFMDFVLSKRGQKVMQQSNYLPSHPEMPALQADLKPG
                     GGRFKRVHYMNPEQEFDRSNEWVEYFQKNFVQ"
     gene            2974..4059
                     /locus_tag="VMZ02_11080"
     CDS             2974..4059
                     /locus_tag="VMZ02_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72523.1"
                     /translation="MTQLPLQRFLALIAILIVLSPAALVSGATVEEIALMNRADRQKI
                     LVEGARKEGKVSWYTTLIVDQVVRPVKEAFEKEYPFIQVEYFRGNSAQLAQRIISENQ
                     AKRYDNDVVDGTVVPTMVRRAGLLQRFNSPFFSEYPAELKDAQGFWGATNLYFFATGY
                     NTRMVKATEVPKTYDDLLNPRWKSQMMWSTSRGSGAPMFVGTILNTMGAEAGKAYLQK
                     LKTQNIAKTTASNRQVLDLTIAGEYPLALQIFNHHAFISKSAGAPVDWQVHEPATATI
                     NNIGLAKNTTRPHSSMLLIDFILSEKGQKVYQQANYLPAHPKVPAKQVDLKPGGGRFK
                     RVYYINPETQFDKGNEWVDYFENVFMK"
     gene            4073..5842
                     /locus_tag="VMZ02_11085"
     CDS             4073..5842
                     /locus_tag="VMZ02_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HUR72524.1"
                     /translation="MAKSQPSESLELPLKSATARRARFVGASRLEVPSVVMICVSVIT
                     AYLVLPPLYSVIQTSLFTTKLTGEIDQFTLRYYEDLLRELQVLGPFLNTFYFSLGSAL
                     LATLLGGSIAWIVVRTDSPLRGLGYFTAFASFGTPFILYTIGWLLLLGKAGPVNYWLK
                     IFLNQPGPVINVYSMSGMILIESLLWSPFVFLMLAAAFRSMDPSLEEASAACGARVWQ
                     TMRRISLRLMLPAFFSVMLLIFIRTFESFEIPALVGLPGDIRVLTTSIYLDAQKLPPR
                     YGSAGAFSVLLMVVVAATLYFYFRLTREGDRYQTVTGKGYRPTLINLGRWRYLTAAAL
                     MAYSIILLVLPFVIILWASVLPFYMQPSLEGLTKLTLKNYATALHFPKITDAIKNSVL
                     LGLGSASFVMVLTLLASWLLVRTKIRGRWLLDLMTTLPLLFPGIVMGLAILRFYLFVP
                     IPVYGTLWILLIAFVTRYIPYGIRYTHSGLLQLHKELEEASYVSGASWFSSMRRIILP
                     LMTPSFLGGWIFIFLLSAKELSMSVLLVSPQTPVVSVAIFELWENAQVGELAAFGVIW
                     TSILVTVAIFYYAFARRYGIQQN"
BASE COUNT         1323 a         1627 c         1674 g         1411 t
ORIGIN      
        1 aatcggcttt agtgacccgc gcgtgcccag ttccgggcaa tcgatctggt catttatgtg
       61 ggagctgaaa ggcgaggagt ttctcaagaa attagtccag caggaactct ttttgacccg
      121 cgatttgcgt cagctcgccg atgcccttgc caaaggcaaa gtcgcgatag ccttcggttt
      181 ggggcgcagt cagaccgatc cattcgtcga tgccggcctg ccgatcaagc cggtgccggc
      241 gcctaaagaa ggcttgccgg cgagtaattc attcggcgtc attggtgtta tcaagaatcc
      301 gcccaacccg aatgcgacga aagtattcgt caattggttt ctcagccgcg aaggccagga
      361 gtggtacggc cggatcatgg agaacggcac gcgccgtctg gacgtcgaga ccaagtggct
      421 caacaagatc ggcgtcagcg cggcaaagga cacgattacc attcaagaat atcatcgttt
      481 gcgcaatcat ctcgaagata aatacaccaa ggtgagagtg ccggcgggaa gatttgccga
      541 gacgattctg aaataagcgt tctaggattt atcgttcgaa aatcaacgag attgcagagc
      601 tcatggagat cagccgatga aaaatttcat taacataagc atcatggccc tagcgctcgt
      661 gattgggagc gctgccacct gcggcgcggc gaccgtggac gaagtcatgc tttataacaa
      721 gcccgaccgg cagaaaattt tgctcgaggg cgccaagaaa gaaggcaaga ttacttggta
      781 tacctcgctg atcatcgacc aggtcgtgcg cccggtcaaa gaagcgttcg aaaaaaagta
      841 tccgttcatt caaatcgaac cgtttcgcgg caactccgag cgcatcgtcc agaaaatgtt
      901 tgccgaatac cagggcaagc gttacgaagt cgacatcatg gacggcaccg tgacggcacc
      961 gatggtcaaa aaaggcggct acctgcagcg gttttactcg ccacaccttg ccgaatatcc
     1021 cgccgagctg aaggaccctc aaggttactg gggagtgagc aacgtttact acttcgcctt
     1081 gggctataac acccgcatgg tcaagcccgg cgaggtgccg aagacttatg aagacttgct
     1141 caacccgcgc tggaagggac aaatgatgtg gtcgaccagc cgcggttcgg gcgcgccgat
     1201 gatgatcggc aatatttttc aatccatggg gcaggaagcc ggcaaagctt atctgcaaaa
     1261 attgaaagcg caaaatgtcg ccaagtccac cgccagcaat cggcaactat tggatctcgt
     1321 gatggccggt gaatacccgc tcgcgctgca tatctttcac caccacgctt atatcagccg
     1381 gaccgcgggc gcgccggtgg actgggcgcc gctggagccg ctctcggcca ctatcaacac
     1441 gatctcgccc gtgacacgtt cgccgcatcc tcacgcggcg atgctgctgc tcgactttat
     1501 tctctcggaa gaagggcaga aggtcattca agcggctaat tatctgccgt ctcatccgaa
     1561 ggttccggcc aaacaagtcg acctcaagcc gggcggcgga aagttcaagc gggcgctcta
     1621 tttcaccccc gactcgcagg tgaatgaagg cgattcctgg gtggactact tcgagaaaaa
     1681 tttcttgaag tagcgactgc cgattcctag cggccaaagc aggcgatggt tcctccgggg
     1741 cgacaatccg aggaggtgag cgatgaagtt ttttcgtgct ggggcggact cgtttcgaac
     1801 gttgcttcgg atggcttcgt tcctagtcgt gcttgcttgc gcgcaaactg cactcgccgc
     1861 ttctgtcgaa gagatcgcat tactcaaatc cgccgaacgc cagaggattc tcgtcgaagg
     1921 cgccaagaaa gagggcaagc ttagttttta cacgaccctt atcatcgatc aggtgatccg
     1981 gccaatgaaa gaagccttcg aaaaagagta cccattcatt cagatggagt atttccgcgg
     2041 taattcggat cgcctagcgc aaaagctctt cgctgagtac caggcaaaac gatacgccgt
     2101 cgatgtcgtc gatggcagct cgacgacgac gatggtccgg cgcgccggct ttcttcagcg
     2161 attttattca ccgcatcttg cggagtatcc ggcagagctc aaagatgccg aaggattctg
     2221 gggcgtgagc aatctttact ttcacaccat cggctacaat acacgcatgg tcaaacccaa
     2281 cgacgttccc aaaacctacg aagatctgct gcatccgcgc tggaagggac agatgatatg
     2341 gtccacaggg cgcggcggtg gcgggccgtt gtttgtcggc aatgtgttgc agagcatggg
     2401 cgagaacgcc ggtaaggctt acttacagaa attgcgcgga caaaacattg ctaaaagcac
     2461 tgccagctcg cgccaggtgc tcgacatggt catcgccggc gagcagccga tcgttctcgt
     2521 gatcttcaat catcatgctt acatcagcaa aaatgccggc gcgccgtcgg agtggcagcc
     2581 gttggagccg gtgcctgcgc gggtcagcac catcggtctg cccaagtttg cgcctcatcc
     2641 gcacgcggcg atgttgttca tggattttgt cctgtccaag cgcggccaga aagtgatgca
     2701 gcagtccaat tatctgccgt cccatccaga aatgccggcg ctgcaagccg acctcaagcc
     2761 tggcggcggc cgcttcaaac gagttcacta catgaatccg gagcaggagt tcgaccggag
     2821 caacgagtgg gtcgagtatt tccagaaaaa cttcgttcaa taaacaccgc cccgaggaaa
     2881 ttttgcgccg ccaattatct tgacaatcca acgttagtca gactagcttc gctgccatat
     2941 gttttgcttt gcctgccaca tggagggatc gcgatgacgc aattgccgtt gcaacgtttt
     3001 ctagcgctca ttgcgatttt gatcgtcttg tctcctgcag cgctggtgag tggcgcgacg
     3061 gtggaagaga tcgctctgat gaaccgcgcc gaccggcaaa aaattcttgt cgagggcgct
     3121 aggaaagaag ggaaagttag ctggtacacg acacttatcg tcgatcaggt ggtgcgaccg
     3181 gtcaaagaag ctttcgaaaa agaatatcca ttcattcagg tggaatattt tcgcggcaat
     3241 tcggcgcagc tggcgcaaag aatcatttcc gaaaaccaag ccaaacgtta tgacaacgat
     3301 gtggtggatg gcacggtggt gccgacgatg gtgcgacggg ccgggttgct gcagcgattt
     3361 aactcgccat ttttttccga gtatccggcc gagctcaagg acgcgcaagg tttctggggc
     3421 gcgaccaatc tttatttttt cgctaccgga tacaatactc gcatggtcaa agcgactgag
     3481 gtgccgaaaa catacgacga cttgctcaac ccgcgttgga agtcgcaaat gatgtggtcg
     3541 accagccgcg gctcgggtgc gccgatgttc gtcggtacga ttctcaacac catgggcgcg
     3601 gaggccggga aagcctattt gcagaaactc aaaacccaaa acattgccaa gactacagcg
     3661 agcaaccggc aggtgctcga tttaaccatc gccggcgagt atccactagc actgcagatc
     3721 tttaatcatc atgcatttat cagcaagtcg gcgggggcgc cggtggattg gcaggtccat
     3781 gagccggcga cggccacgat caataacatc ggactggcca aaaacacgac ccgtccccat
     3841 tcatccatgc tgctcatcga ctttattctg tccgagaaag gacagaaggt ttaccaacag
     3901 gcgaattatt tgccggctca tcccaaggtg cccgccaagc aggtcgattt gaaaccgggc
     3961 ggcgggcggt tcaagcgcgt ttattacatc aatcccgaga cgcaattcga caaaggtaac
     4021 gagtgggtcg attattttga aaatgtgttc atgaaatagt cgcagcgaaa cgatggcaaa
     4081 atcccaaccc agcgaatctt tggaattgcc gctcaagagt gcgaccgcgc ggagagctcg
     4141 tttcgtgggc gcctcgcgcc ttgaagtgcc gagcgtcgtg atgatctgcg tatcggtgat
     4201 caccgcctat ttggtgctgc cgccgcttta ttcggttatc caaaccagtc tttttacgac
     4261 caagctcacc ggcgagatcg atcaatttac gctgcgctac tacgaagacc tgctacgcga
     4321 gctccaggtc ctcgggccgt ttctcaatac tttttatttt tcactcggca gcgcgctgtt
     4381 ggcgacgctg ctcggcggtt cgatcgcctg gatagtcgtt cgtaccgaca gtccactgcg
     4441 cggcttgggc tactttactg cgtttgcttc cttcggtacg ccattcattc tctacacgat
     4501 cggctggctg ctcctgctcg gcaaggcggg accagtcaac tactggctaa agatttttct
     4561 gaatcaaccg ggacccgtca tcaacgtata ctcgatgtcc ggaatgattt tgatcgagtc
     4621 gctgctttgg tcgcccttcg tctttctcat gctcgcggca gcgtttcgct cgatggaccc
     4681 ctcgctcgaa gaggcgtcgg cggcgtgcgg tgcccgggtc tggcaaacga tgcggcggat
     4741 ttccttgcga ctgatgctgc cggcgttctt ctcggtcatg ttattgattt tcatacgcac
     4801 ctttgagtcg ttcgagatcc ccgcgttggt cggcttgccg ggggacatcc gggtgctgac
     4861 gacgtcgatc tatctcgacg cacaaaaact gccgccgcgt tatggcagcg caggggcatt
     4921 ttcagtgttg ttgatggtgg tggtggcggc gacgctgtat ttctactttc gcctgacgcg
     4981 cgagggcgac cgctaccaaa cagtgaccgg taagggctac cggccgacgc tgataaatct
     5041 cggccgttgg cgctatctga cggcggcagc actcatggcc tattcaataa tccttctggt
     5101 gttgccgttt gtgatcattc tctgggcatc ggtgttgccg ttttacatgc aaccgtcgct
     5161 cgaaggtctg acgaaactga cactcaaaaa ttatgctacg gcactgcact ttccgaagat
     5221 taccgacgcg attaagaata gtgtgctgct tggtctcggc agcgccagct tcgtcatggt
     5281 gttgactctg ctcgcgtcct ggttgctggt gcgcaccaaa attcgcgggc gctggctgct
     5341 cgatctcatg acgacgctgc cgctgctatt ccccggtatc gtcatgggat tggcgatttt
     5401 gcgcttctat ctatttgtgc caatcccggt gtatgggacc ttgtggattt tgttgatcgc
     5461 cttcgtcacg cgctacatac cgtatggcat ccgctatact cattcgggtt tattgcagtt
     5521 acacaaggag ctggaagagg cgtcctatgt atccggcgcg tcatggttca gttccatgcg
     5581 gcgcatcatt ttgccgctga tgacgccgtc gtttctcggc ggttggattt ttatcttttt
     5641 gctgtccgcc aaagagctgt ccatgtcggt cttactcgtc agtccgcaaa cgccggtagt
     5701 gtcggtggcg atcttcgagc tttgggaaaa tgctcaggtc ggtgaactcg ccgccttcgg
     5761 cgtgatctgg acctctattc tcgtcacggt ggcgattttt tactacgctt tcgcgcgccg
     5821 ctacggtatt cagcagaatt gaagtgacgc tgccgactcg tacctggggc tcgctgtgaa
     5881 cggcgagtcg cttcaatcga aaatcgtctt cgagttgttt gcgcttgaac cgcgaagcat
     5941 agcgacactg acgaggcgtt accgaacatc gattccgatt ctttgtctta ttttgatcat
     6001 tggcgttgtg ttcagtgacg tgggcgcgtc agatg
//
LOCUS       DATLCH010000370         5512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8038065, whole genome shotgun sequence.
ACCESSION   DATLCH010000370 DATLCH010000000
VERSION     DATLCH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8038065"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1033)
                     /locus_tag="VMZ02_11090"
     CDS             complement(44..1033)
                     /locus_tag="VMZ02_11090"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72525.1"
                     /translation="MRLTGKIAALCFFILTLADAGHAAESLRKMIVGQAGVNPGAGLF
                     SIAVNQGLYKKHGLDVEIIKTNTTAAVQAMLANKMHMATGAGMAAFVTANLEGAPGFT
                     LVGSWVNVFPYKIMAQKGIKKVEDLKGKTGHVGAPFGTIPDTALRFALGRLKIDADKD
                     VKLVQHSNADPASILAAMEKGEVQFGILAPPFDLIAERRGYEMLLALPGLGIPWQQNG
                     EIVPTAYLKANRDNVVRFLRATADASKFYFDNKDKTLEIMTEYLGRPRDEAEYAYNQF
                     KRWADKNHRPQVDTIKTTLEAIKKNTPKAAGANPASFVDTSIADQLIKEGYYK"
     gene            complement(1054..1869)
                     /locus_tag="VMZ02_11095"
     CDS             complement(1054..1869)
                     /locus_tag="VMZ02_11095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR72526.1"
                     /translation="MVSVDESNPLTTNRHWQRTREILSNWLGVIGVVAFFLAWELSTR
                     LEIINPFYFPPFSKIIVKGYELFANGSIWEHIWFSLTNFLIGFAISVILGVVMGVPMG
                     WYRAVHKTFDPLLSGIYATPLIALLPLIIMMFGLGSLSKIIMTILAAVFPILINTMVG
                     IANTDNRLITMSRAFGARDSQIFMKVSLPGSLPYIVAGMRVALGRALVYIVVAEQYGA
                     AMGLGYLSSVAAQRFQMATMFVPIVIIAGLGAGLNETLKAIEHRLDKWKPQKA"
     gene            complement(2072..2887)
                     /locus_tag="VMZ02_11100"
     CDS             complement(2072..2887)
                     /locus_tag="VMZ02_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HUR72527.1"
                     /translation="MAKDNRMLTPVKIDDRIDAYLGIPEGIGKRPVIIHMHERYGIVQ
                     HTLDLGEKYVKAGYVTIVPDYFSRFTGDRKALAEGNDRCELDDEVVLSDTDVIMDYMR
                     SVAQADMSKVAMSGVCQTGRQPILVGAKRDYLCAAVVLYGAVYEADWKSHPMRPESIE
                     TLMQKMQCPLTAVFGELDNLIHLDNIVRMAAVLAAAKKSFGIRVYPDAPHGFLNDTMP
                     GRYRQLQSDAAWNQIVSFLKSVFAGEWKKDRAIWRFEADTSLDYDFSAMKRWE"
     gene            complement(3169..3744)
                     /locus_tag="VMZ02_11105"
     CDS             complement(3169..3744)
                     /locus_tag="VMZ02_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72528.1"
                     /translation="MNRRGILLLSALLIWHPPLAAAAEILDIAACTAVTTADADKFIG
                     APLDVTKVDKKIMINAPWSHDSLCTYMPQGVNSDDPTKIARFLDVSLRFFPTVEAAQA
                     IHQATLDQFHKMAASPDAPFKITAINPIEGIAAQAFYVELQADPGSEYKSVMIMFVKD
                     TIGGAVSAWKKPEPSLELSKAVLQHVLLKLP"
     gene            complement(3816..4403)
                     /locus_tag="VMZ02_11110"
     CDS             complement(3816..4403)
                     /locus_tag="VMZ02_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72529.1"
                     /translation="MIRGYVALLVFYVSLALVPGRSFGQIGQTSAPTACGIITGDDAQ
                     KFVGGPLDVKEQASVPINNAPGAYNSVCTYVARGTDVEKALTAPRLLDVTLHFLDSAE
                     NMALIYENSVITYTHLANTPDPRFKNPSINYLGGFGDKAFVFEAVTDPVSGYKSALIV
                     FYKGRIGGSVAAWKKPESAVDTSKAVLKFILSRLP"
     gene            complement(4649..>5512)
                     /locus_tag="VMZ02_11115"
     CDS             complement(4649..>5512)
                     /locus_tag="VMZ02_11115"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72530.1"
                     /translation="VAKEVGLFKKYNLDPTLVFTTGGSLGIQAMLGGDVAMTLSDGSS
                     SVVSHLAGVDIVVIASLLNTFPYSLISLPEIKKVDQLRGTKIAVSRFGSATDLSVKMA
                     LAKVGLNADKDVTLLQIGVQTARYAALQSKNVQATIITPPFTLTARKAGMNTLLDMSE
                     LNIPFQLTGLLALRPYLKNNPDTVLSVIRTLSEAIYFYKREKETTLKILGRYLKTDDR
                     EALEETHREIAIKSMPEKPYPSLAGIKTILDETALRNPKAKGLRPEDFVDSSFVKKVD
                     DEGLFERLYKK"
BASE COUNT         1197 a         1460 c         1547 g         1308 t
ORIGIN      
        1 gcgatcgtcc aagataatct tctatcctcg atcgatgact tgattacttg tagtagcctt
       61 ccttgatcag ctggtcggca atgctggtgt cgacaaaaga ggcgggattg gcgccggcgg
      121 ctttgggtgt gttcttctta attgcttcga gagtcgtctt gatcgtgtcc acttgcgggc
      181 ggtgattttt atcggcccag cgtttgaatt gattataggc gtactccgct tcgtcgcgcg
      241 gccggccgag atactcggtc atgatttcga gcgtcttatc cttgttgtca aagtaaaatt
      301 tcgaagcatc ggcggtggcg cgcagaaagc ggacgacgtt gtcgcgattg gccttcaagt
      361 aggcggtcgg cacgatctcg ccattttgct gccagggaat tcccaagccg ggcagcgcca
      421 ggagcatctc gtaaccgcgc cgttcggcga tcaggtcgaa aggcggtgcc aaaatgccga
      481 actgcacttc gcccttttcc attgcggcca atatactggc tgggtcggcg ttcgaatgct
      541 gcaccagctt gacgtccttg tcggcgtcga ttttcaatcg ccccaaagca aaacgcagcg
      601 cagtgtctgg aatcgtgccg aagggcgcgc cgacatggcc ggtcttgcct ttgagatctt
      661 cgactttttt gatgcccttc tgtgccatga ttttatacgg aaagacattt acccaggagc
      721 cgaccagcgt aaatccaggc gcgccttcaa gattggcggt aacaaaggcc gccatgccgg
      781 cgcccgtggc catgtgcatt ttattggcga gcatcgcctg caccgccgcg gtggtgttgg
      841 tcttgatgat ctcgacgtcg aggccgtgct tcttatacag cccctgattg acggcgatgg
      901 agaataagcc ggcaccgggg tttacgccgg cctggccgac gatcattttg cgcagcgatt
      961 cggcagcgtg gccagcgtcg gcgagagtca agataaaaaa acacagagcg gcgatttttc
     1021 cggtcaacct cattcaatcc tccgagtcgt tatttaagct ttttgcggtt tccacttgtc
     1081 gagccggtgc tcgatcgctt tgagggtctc gttcaagccg gcgcccaggc cggcgatgat
     1141 gacgatggga acgaacatcg tggccatttg aaatcgttgc gcggccaccg aactcaagta
     1201 accgagtccc atagccgcgc cgtattgttc ggcaacgacg atatacacca gcgcgcgtcc
     1261 caacgcgacg cgcatcccgg cgacgatata gggcagggag ccgggcagcg acaccttcat
     1321 gaaaatctgg ctgtcgcgcg cgccgaaagc ccgcgacatc gtaatcaagc ggttgtcggt
     1381 gttggcgatg ccgaccatgg tgttgatgag aatcggaaac accgcggcga gaatagtcat
     1441 gatgattttc gatagcgagc ccaagccgaa catcatgatg atcaacggca gcagagcgat
     1501 cagcggcgtc gcatagatgc cggagagcag cggatcgaag gtcttgtgta ccgcccggta
     1561 ccaacccatg ggcacgccca tgacgacacc gagaatcacc gagatcgcaa agccgatcaa
     1621 aaaattggtc aggctaaacc atatgtgctc ccaaatggag ccgttggcga aaagctcgta
     1681 acccttgacg ataattttgc tgaacggcgg aaagtagaat gggttgataa tctcaaggcg
     1741 cgtcgaaagt tcccaggcga ggaagaacgc gacaacgccg atgactccga gccagttcga
     1801 gaggatctcg cgggtgcgct gccagtgcct gttggtggtc agcggattcg attcgtcgac
     1861 ggagaccaca tcgaaggcgt cttgttctgt ttttgccgta aacgctcctt cggttcgggt
     1921 gacgcgaatg tatgcgcggc tgcatccgct gtcaagctgg cttgtcagtg gaactacaga
     1981 ttccagatgt caaatccatt aaggatcacc agcatcaaat cgccaatttg gaatctggca
     2041 tttgccattt ggaaggcggc gcttagcgct gctattccca acgcttcatc gcgctgaaat
     2101 cataatccaa tgaggtgtca gcctcgaagc gccaaatcgc ccggtccttt ttccattcgc
     2161 cggcgaatac cgacttcaaa aacgacacga tttgattcca tgcagcgtcg ctttgcagct
     2221 gacgatagcg accgggcatc gtgtcgttga gaaaaccatg cggtgcatcg ggatagaccc
     2281 ggatgccgaa gctttttttc gccgccgcca gcactgccgc catgcgcacg atgttgtcga
     2341 gatgaattaa attatcgagc tcaccgaaga ccgccgtgag cgggcactgc attttttgca
     2401 tcagcgtttc aatggactca ggccgcatcg gatgcgactt ccagtcggcc tcataaactg
     2461 cgccgtagag cacgacagcg gcgcaaagat aatcgcgctt ggcgccgacc aagatcggtt
     2521 gccgcccggt ctggcaaacc cccgacatcg cgactttact catatcggcc tgggcgacgc
     2581 tgcgcatgta gtccatgatt acgtcggtgt ccgacaggac gacttcgtcg tcgagttcgc
     2641 agcggtcgtt tccttcggcg agcgccttgc gatcacccgt gaagcgggaa aagtaatcgg
     2701 gcacgatggt aacatagccg gctttgacat acttctcgcc caaatccagt gtgtgctgca
     2761 cgataccgta gcgttcgtgc atgtggatga ttaccggtcg tttgccgatg ccttcgggga
     2821 tgccgagata agcgtcgatg cgatcgtcga tttttactgg tgtaagcatg cgattgtcct
     2881 ttgccacgtt tttgaaaact ccatcgtcct aacattgacg acagagattt tctactgttt
     2941 cctgcaaatc tgggcagcct cgcttgtgag gatatcctgc tcccatgagt gaccatggct
     3001 gacactcgta cagaatataa agttggagga tctccctcga gctgatcctc tcttcaattc
     3061 gtcattcccg cgcaggcgtg aatttaggtt tgttctcgcc gaattatcct gataccccga
     3121 tttcgcgggt atgatggagt tacaagaaca tgattgctcg acgacttgct agggcaattt
     3181 taacaagaca tgttgcagga cggctttact gagctccaac gatggctcgg gtttcttcca
     3241 cgcactgacg gccccgccga tggtgtcttt gacgaacata atcatcacag acttgtattc
     3301 tgatcccggg tcagcttgga gctcgacgta aaaggcttgc gcggcaatcc cttcgatggg
     3361 attgatagca gtgattttga atggagcgtc ggggctagcg gccatcttgt ggaactgatc
     3421 caaggttgcc tgatggatcg cttgcgccgc ttccacagtg ggaaaaaatc tcaagctcac
     3481 gtcaagaaag cgcgcaatct ttgtcggatc gtcgctgttt actccttgcg gcatataggt
     3541 gcacagggag tcatggctcc acggcgcatt gatcatgatt ttcttgtcga ccttggtcac
     3601 gtcgagaggc gcgccgatga atttatcggc gtcggccgta gttacggctg tacaggcggc
     3661 aatatctaag atttccgccg cggcggcgag cggaggatgc caaattagca gcgcgctcag
     3721 cagcaaaatt ccacgtctgt tcatcgatga gctctgggtt ggatggccgg tttacgagaa
     3781 cacgctgtca tgcgaaagcc gcggctatca cagggtcaag gcagccggct gaggatgaat
     3841 ttcaagaccg ccttgctggt atccacagcg gactccggtt tcttccacgc ggcgacgcta
     3901 ccgccgatcc ggcctttgta gaaaactatc agggcggatt tgtaaccgct cacgggatcg
     3961 gtaaccgcct caaagacaaa ggccttgtcg ccgaagccgc cgagataatt gattgacgga
     4021 tttttgaatc ggggatccgg agtgttggct aagtgagtgt aggtgatgac cgagttctca
     4081 tagattaacg ccatattctc ggctgaatcc aaaaaatgca gtgtgacatc gagtaaacgc
     4141 ggcgccgtca gcgctttctc cacgtcggtg ccgcgggcga cataagtgca taccgagttg
     4201 tacgcccctg gtgcattatt aatcggcacg ctcgcctgtt ccttgacgtc gagcggtcca
     4261 ccgacgaatt tttgcgcatc atcgcccgtg attatgccgc aggcggtcgg cgccgacgtc
     4321 tggccaattt ggccgaaaga ccgacctggg acgagcgcga gagagacgta aaaaaccagc
     4381 aatgcgacat atcccctgat cacccttcac ctctttcgat gcaatgtcct gctggccggc
     4441 gatgtttaaa caccgagagt atttccgata ttcactgttt gcgcgccgtc accagcgtcg
     4501 aattgcaagc tgaacgagct tgccgacgat ttcaccggca gccgttacgg cgcttcaaca
     4561 tcgagatatt cagcgggtcc ggagaagcgg ccgaccgaca tcaaacaata atgaatgatt
     4621 gtagcaaatc tcgccgtgcc tcgacaggtt atttcttgta gagccgctcg aataatccct
     4681 cgtcgtcgac tttcttgacg aagctcgaat cgacgaagtc ctcaggtctc aggcctttgg
     4741 ctttaggatt gcgaagagcg gtctcgtcga gaatggtttt gattcccgcc aggcttgggt
     4801 agggtttttc cggcatcgac ttgatggcga tctctcgatg ggtctcttcg agcgcctcgc
     4861 gatcgtcggt ttttaaatat ctgccgagta ttttgagggt tgtttctttt tcgcgcttgt
     4921 agaaatagat cgcttcggag agtgttcgga tcaccgacaa aacagtgtca gggttgttct
     4981 tcaggtaagg acgcaatgcc aacaagccgg tcaattgaaa aggaatgttg agctcggaca
     5041 tgtcgagcag cgtattcatg ccggctttgc gagcggtgag tgtgaacggc ggcgtgatga
     5101 tcgttgcctg gacatttttt gattgcagcg cggcatagcg cgcggtttgc acgccgatct
     5161 gcagcaacgt gacatctttg tcggcattca agccgacctt cgccaaggcc atctttacgc
     5221 ttaagtcggt cgcgctgccg aagcgactga cggcgatttt tgtccctctg agctgatcga
     5281 ctttcttgat ctcgggaagt gagatcaaac tgtaagggaa ggtatttagc agactggcaa
     5341 tgacgacaat atcgacgccg gccaaatggc tgacgactga agacgatccg tccgacaacg
     5401 tcatggcaac atcaccgccg agcatggctt ggatgcccag cgatccgccg gtggtaaaca
     5461 ccagtgtcgg gtcgaggttg tattttttga acagaccgac ttccttggcg ac
//
LOCUS       DATLCH010000371         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10309008, whole genome shotgun sequence.
ACCESSION   DATLCH010000371 DATLCH010000000
VERSION     DATLCH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10309008"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VMZ02_11120"
     CDS             <1..268
                     /locus_tag="VMZ02_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72531.1"
                     /translation="LKRNRADSERIIGKYMKISDPKLMATEFDFVASLMPDYIAPTLE
                     GTKLILENFGKEYPDAPKRDPKEFADGSIIERLKQEKFAESLKF"
     gene            459..593
                     /locus_tag="VMZ02_11125"
     CDS             459..593
                     /locus_tag="VMZ02_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72532.1"
                     /translation="MNLIIRHMDYIQQRLHAFHALVNLPPTIYLLAHLKETPWDKFSG
                     "
     gene            615..983
                     /locus_tag="VMZ02_11130"
     CDS             615..983
                     /locus_tag="VMZ02_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HUR72533.1"
                     /translation="MARIRHLAILTEDVEKLVKFYTEGFGLKVIHGVGTATYLTDGHI
                     NLAIIPIGPEREIEGPQLRTGINHFGFEVEDVAAVREACKGLDAAEDVRKRPPNREAE
                     YRVLDPDGNPVDLSQHGWPH"
     gene            complement(1216..1647)
                     /locus_tag="VMZ02_11135"
     CDS             complement(1216..1647)
                     /locus_tag="VMZ02_11135"
                     /inference="COORDINATES: protein motif:HMM:NF040144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HUR72534.1"
                     /translation="MTTRAKSFVALLLSALLPGLGQIYNHERKKGWVILACCVALAIA
                     IYWLDDFNAITAALALLLLWSSAIIDAYKVAKSAGHAAEFYYAKSYVVAMLLLVGPLA
                     LPLLWKSPNFSNSARWIWTVIVVGTALLFVATPYLLKSLIG"
     gene            complement(1801..>2601)
                     /locus_tag="VMZ02_11140"
     CDS             complement(1801..>2601)
                     /locus_tag="VMZ02_11140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72535.1"
                     /translation="GAMGHHFGSPLVSRYWTEACNDLIARVAKTWPDKVSPVCQLPQS
                     PGVSPTEWADELDRCVKDLGFISCNVNPDVAGGKEPLTPSMGSEWWYPLWEKLVALDV
                     PCTVHASASLNPAMHLTNSYYIAHHHSAAVEILKSRVFKDFPKLKLIIPHGGGAIPYQ
                     WNRQRGMHVKEGLEPFEEAARRVYWDMAIYDKESMELLIKRVGADRVLFATEMFGAVN
                     AIDPGTGRNFEDLVPIFKSVEGLTEQDRYNITEGNARRLYSRLFADKK"
BASE COUNT          617 a          746 c          689 g          549 t
ORIGIN      
        1 cctgaaacgc aaccgcgccg acagcgaacg gattatcggc aagtatatga agatcagcga
       61 tcccaaattg atggccaccg agttcgactt cgtcgcctcg ctgatgcccg actatatcgc
      121 gccgacgctg gaaggaacca agctgatcct ggaaaacttc ggcaaagaat accccgacgc
      181 tcccaagcgc gaccccaagg agttcgccga cggctcgatc atcgaaaggt tgaagcagga
      241 aaagtttgct gagagtctta agttctaaac acgcgctccc aaatcactat cgaatcgaga
      301 cggtgaagct acaagctttc gattatgcca gctccatcat aggtctgacg agtaccttgc
      361 tgatcctcgg agcttttggt gaagtcgcta atattttact agtggtttcc accgcgattt
      421 ccaccatgct gtgtctcttt tcaattgggc cattaagcat gaatctgata atacgccaca
      481 tggattatat ccaacaacgt cttcatgcgt ttcacgcgct tgtgaatcta ccgccgacga
      541 tctatttact cgcccatctt aaggaaacac cctgggataa gttttctgga taaagtcgcc
      601 cataggaggt tatcatggca cgcattcgcc atctcgcgat actcaccgaa gacgtggaaa
      661 agttggttaa gttctatacc gagggcttcg gcctgaaggt cattcacggc gttggcacgg
      721 cgacttatct caccgacggc catatcaacc tggcgatcat cccgatcggc ccggaacgcg
      781 agatcgaggg accgcagctt cggaccggca tcaaccactt cggtttcgaa gtcgaagatg
      841 ttgccgccgt gcgtgaagcc tgcaagggac tcgatgccgc ggaagatgtg agaaagcgcc
      901 cgcccaatcg cgaagccgaa taccgcgtcc tcgacccaga cggcaatcct gtcgatttat
      961 cgcagcatgg ctggccgcac tgaggttgaa tccaacttgt gacttcagca actaaaaagc
     1021 cctcggcagt tcctcgaggg atcgtcaata ggtcgaccaa aagtcgattc ttgtttttcg
     1081 tcattccggc gcaggccgga atccaggctt tatttggccc tgcagaataa gcgacgatgg
     1141 ataccggctt tcgccggtat gacaaaccca cccctggatc gagaaccagg gacttcaaca
     1201 atccggaaag agccattaac cgattagcga cttcaataaa tacggcgtcg caacaaacag
     1261 cagcgcggtg ccaaccacga tcaccgtcca aatccagcgc gcgctgtttg aaaagttcgg
     1321 acttttccat agcaatggca aagccagtgg gccgacgagc agcaacatag ccactacgta
     1381 actcttggcg taatagaatt ctgccgcatg cccggcagac ttggcgactt tgtaggcgtc
     1441 gattatggcg ctcgaccaca agagcaacag cgcgagcgcg gcagtgatgg cgttgaaatc
     1501 atcgagccag taaatggcga tggccagagc aacacagcag gcgagaatca cccagccttt
     1561 tttgcgctcg tgattataga tttgccccag accgggcagg agcgccgata atagcagcgc
     1621 aacaaacgac tttgctcgcg tggtcatgac ctgacgcccg ctagtgagct gctgtcatgg
     1681 gaaaccaaag cgagcaatcg aaaaatcatt tctgacaggc aagcgatctt gcggtgagtc
     1741 tcagatcaat ccatcgcccg ttcccagaca accgctaagg acaaatgcga cggcaagcgc
     1801 ctacttcttg tccgcgaata gccgcgaata caatctgcgc gcattgccct cagtgatgtt
     1861 gtagcgatcc tgctccgtca atccttcgac ggacttgaag atcggcacca ggtcttcgaa
     1921 gtttcgaccg gtcccgggat cgatggcgtt gaccgcgccg aacatctcgg tggcgaacaa
     1981 tacccggtcg gcgccgacgc gcttgatcaa cagctccatg gactctttgt cgtaaatcgc
     2041 catgtcccaa tacacccggc gcgccgcttc ttcgaagggc tcgagtcctt ctttgacatg
     2101 catgccgcgc tggcgattcc actgatacgg aatcgccccg ccgccgtgcg gaatgatcag
     2161 tttcaacttg gggaaatcct tgaacacacg cgacttgaga atctccaccg cggcggagtg
     2221 gtgatgcgcg atgtaatagg aattggtcaa gtgcatcgcc gggttgagcg atgcgctggc
     2281 gtgcaccgtg cacggcacat cgagcgcaac gagtttttcc caaagcggat accaccactc
     2341 gctgcccatg gatggcgtca gaggttcttt tccaccggcg acgtccggat tgacgttgca
     2401 gctgatgaaa cccaagtctt ttacgcagcg gtcgagctcg tcggcccatt ctgtcgggct
     2461 cacccctggc gactgcggga gctggcacac cggcgacact ttgtcgggcc aggttttggc
     2521 gactcgcgcg atcaagtcgt tgcatgcttc ggtccagtag cggctgacga gcggcgaacc
     2581 gaagtgatgg cccatggcgc c
//
LOCUS       DATLCH010000372         6059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8098064, whole genome shotgun sequence.
ACCESSION   DATLCH010000372 DATLCH010000000
VERSION     DATLCH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8098064"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VMZ02_11145"
     CDS             <1..276
                     /locus_tag="VMZ02_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72536.1"
                     /translation="QQLVDYYLQKSIELSGRSLPRAQFYEIYRLSAIQRDLKVVGRFY
                     FLDLVKGKPGYMKFIPPTVRRLQRNLSQVAQTREILPLLNEHFEAML"
     gene            282..1016
                     /locus_tag="VMZ02_11150"
     CDS             282..1016
                     /locus_tag="VMZ02_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HUR72537.1"
                     /translation="MVLAAGQGTRLRPVTDRMPKALVQVAGRPMIEFALLLLRHYGVH
                     EIVINLHHFGTQIEDHLGNGQRFDVNISYSHEPELLETGGGLLKAKAFLQQDTFIVIN
                     TDALIDIDLSAVLKNHRQNNAAATLVLRPDADADRYGSIDIDADGRICRFLEQRSQVE
                     PIGAVKKLMFTGVQILEPRVFEYMDGCGSKKFSTTRETYPRMVHAGERVFGYCFEGFW
                     QDLGTRERITEAEQKLTSGHAQLHFL"
     gene            1154..2095
                     /locus_tag="VMZ02_11155"
     CDS             1154..2095
                     /locus_tag="VMZ02_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HUR72538.1"
                     /translation="MCQSVKPTVSEFMLEKILSDLLQNQNPDGGWGALRGKRSNTEST
                     SFAAIALMALGENPTAAERRKRAIDWFVRQQNQDHSWPLNEATRGGSWTTALAISALT
                     DSAENHDRVLGAARWLLEQEGSKPGILAEIILWAKGRSGVNKVNNDLIGWSWVPNSFS
                     WVEPTSYALIAVKKLRARLAGTSVDERIRQGDAMIYDRMCNGGGWNYGNSKVLDYALW
                     PYPDITAVALIAMQDHAREKANQESLQILNKTARETDSGLALCWAAICLNVYGQDNSE
                     FQKQIEKRFATSAFLGETKSLALAMIALAGKRNPFRI"
     gene            2112..3104
                     /locus_tag="VMZ02_11160"
     CDS             2112..3104
                     /locus_tag="VMZ02_11160"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HUR72539.1"
                     /translation="MRRRDFLKYTTLASIGTAVGGCQSAGRWQREAFRKKTSSRVAIL
                     AAKAYDQPLKDVILRGIKLCGLQVAGKRIVLKPNLVEFDPKGVINTNPAVIEAATDAF
                     RTLGAAEVIVAEGPGHRRDNEYLLSASGLYDVLREHKVRYVDLNTDDIRATPVRSSYT
                     SLAQLYLPETLFNADLLVSMPKLKTHHWAGVTLSMKNLFGIIPGAVYGWPKNILHWQG
                     IDNSILDINSSLPLPQFAIVDGIVGMEGNGPLQGQAKSTGVLIFGDDFVSVDASAARL
                     MKLEPRKINHLAKADAFLGNLANEKIAQIGERLESLQQDFEIIDSLKDLKKITG"
     gene            complement(3144..3725)
                     /locus_tag="VMZ02_11165"
     CDS             complement(3144..3725)
                     /locus_tag="VMZ02_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HUR72540.1"
                     /translation="MDGIEFPMQYFHNYKAHRARILMLSICAWSLVVGVGCSSVGSGE
                     RATAELKDKDGKSVGVASFTEAGGGVRVRLEVKGLSAGLHAVHIHAVGKCETPGFVSA
                     GGHFNPAQKKHGYKSPDGAHAGDLPNMLVAKDGSGRFEALTDAVTLRSGSTSVFDADG
                     SALIVHVGIDDHVTDPTGNAGDRAACGVITARK"
     gene            3909..4559
                     /locus_tag="VMZ02_11170"
     CDS             3909..4559
                     /locus_tag="VMZ02_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HUR72541.1"
                     /translation="MRALSQRALALLLSFSAGVLLGAVFFHMLPEVGSLLGANLGLPI
                     LAGFLLIFVMERFVFVHACEEHDCDIHQMGIPAFLGISLHSLLDGVALGAGLILPQLG
                     PVVLLAVIIHKVPDSMSISSILLSAGWDRHKVGKLNLLFSLTTPIGALLAFLSFRVLS
                     PENVAIAVGLSAGTFLAIATADILPHVHRIEQRNPMTLVFLAIGLAVSWMGTLLAH"
     gene            complement(4586..5302)
                     /locus_tag="VMZ02_11175"
     CDS             complement(4586..5302)
                     /locus_tag="VMZ02_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HUR72542.1"
                     /translation="MIVPAYNEAERLPSTLSRLQQYLSNKSYRFEVIVVLDGPSDASA
                     VVLRSMANEISHLKIIDRSHNRGKGFSVTEGMLAARGQIRLFTDADNSTDIAHFDQMR
                     PLFDQGCDVVIASRNAADVAGAAQVVSQAWHKRAIGKVGNRIVQLAAVPGIWDTQCGF
                     KAFRAEAAEKIFSQTTIDRWGFDIEVLALAQRLNYKIGIIPARWLNDDRSHVKMSDYL
                     HVLGDTFTVRRNLLAGKYKV"
     gene            complement(5536..>6059)
                     /locus_tag="VMZ02_11180"
     CDS             complement(5536..>6059)
                     /locus_tag="VMZ02_11180"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HUR72543.1"
                     /translation="DAHESAARLLQEQGKIAGAVEHYQYVIRLKPDLIDARENLAAGL
                     VNQGRIDEAVQHFRKTLELAPQRNGTRIKLGTVLAIAGRLAEATDVFTFAARADPEDG
                     RILLQLGRVVAAQGDLTQAVHHFTKAARLRPEDAEVHESLGSALSELGNKDEAAKHLR
                     EALRILRSAPAAQ"
BASE COUNT         1385 a         1692 c         1608 g         1374 t
ORIGIN      
        1 cagcagctgg tcgattacta tctccaaaaa agtatcgaac tgtccggccg tagcctgccg
       61 cgcgcgcaat tttatgaaat ttaccgattg tcagctatac aacgcgatct caaggtagtc
      121 ggccgcttct attttctcga cttggtcaaa ggcaaaccgg gatacatgaa atttatcccg
      181 cccaccgtac gtcggcttca gcgcaatttg tcgcaagtgg cgcagactcg agagattttg
      241 ccgctcttga acgaacattt tgaggcaatg ctgtgaaagc gatggtgttg gccgccggac
      301 aaggcacgcg cctgcgcccg gtcaccgatc gcatgcccaa agcgctggtt caggtggcag
      361 ggcggccgat gatcgaattt gccttgctgt tgctgcgcca ctacggcgtc cacgaaatcg
      421 tcatcaactt gcaccatttt ggtacgcaga tcgaagatca tctaggcaat ggccaaagat
      481 tcgacgtgaa catcagctat tcgcatgagc ccgagctgct ggaaaccggc ggcggtctgc
      541 tcaaagccaa agcgttttta cagcaggata ctttcatagt catcaacacc gacgccttga
      601 ttgatatcga tttgtctgcg gtgctaaaaa atcatcggca gaacaatgcg gcggcaacgc
      661 tggtgctacg acccgatgcc gatgccgacc gttatggctc catagacatc gatgctgacg
      721 gccgcatctg ccgttttctc gaacagcggt ctcaggtcga gccaattggc gccgttaaaa
      781 aattaatgtt caccggcgtg cagatactcg aaccgcgggt atttgagtac atggatggct
      841 gcggttccaa aaagttcagc acgaccagag aaacctatcc gcgcatggtg cacgcggggg
      901 agcgagtttt tggctattgc ttcgaaggct tttggcagga tctcggcacg cgggaacgga
      961 tcaccgaagc ggagcaaaag ctcaccagtg gccatgcaca gttgcatttt ttatgagttt
     1021 cgcaaggcga tctgagcagc tgtacatttc ccggtgccat accaacaaat tgacaaaatt
     1081 ttcctgcgca gctcgccgaa tcgattgttt caccgatata ttgggcaaat tttagcggcg
     1141 gcaaaattgc ttaatttgtc aatctgtaaa gccaaccgtc agcgaattca tgctcgagaa
     1201 aattcttagc gatctactgc aaaaccagaa tccggatggc ggctggggtg ccctgcgtgg
     1261 caaacgcagt aatactgaat cgacttcttt tgccgccatt gcattaatgg cactcggcga
     1321 gaaccccacc gcagcggaac ggcgcaaacg cgccatcgat tggttcgtcc ggcaacaaaa
     1381 ccaagaccac agctggccac tcaacgaagc cacccgcggg ggctcttgga ccacggcact
     1441 ggcgatcagc gctctcacgg atagcgcgga gaaccacgat cgcgtgctcg gggcggcccg
     1501 ttggttgctg gagcaagagg gcagcaagcc cggcattctc gctgagatca ttttatgggc
     1561 gaaaggccgg tcgggcgtca acaaagtcaa caacgatctc atcggctggt cttgggtacc
     1621 gaattcattt agctgggtgg aaccgaccag ctatgccttg atcgccgtaa aaaaattgcg
     1681 cgctcgcctc gccggtacaa gtgtggacga gcgcatccgc caaggcgacg ccatgatcta
     1741 cgatcgcatg tgcaatggcg gcggttggaa ctacggcaac tccaaagtct tggattatgc
     1801 gctctggccc tatccggaca ttacggcggt cgcgttgatt gcgatgcaag atcatgcacg
     1861 cgagaaagcc aatcaggaaa gtttgcagat cctaaacaag accgcccgcg agaccgattc
     1921 cggtttagcg ctttgctggg cggcgatttg tctcaatgtt tatggccagg acaattccga
     1981 gtttcagaaa cagatcgaga agcgctttgc gaccagcgca ttcctcggcg aaaccaagag
     2041 tctggcgttg gcgatgattg ccttggccgg gaaacgcaat ccatttcgca tataggattc
     2101 cagagttaat catgcgccgc agagattttc ttaaatacac taccctcgct tccatcggca
     2161 cggctgtcgg cggctgccaa tcggcggggc gctggcagcg cgaagcgttt cgcaaaaaaa
     2221 ccagctcgcg ggttgccatc ttggcagcca aggcttacga ccagccgctc aaggacgtaa
     2281 tcctgcgcgg catcaagctt tgcggactgc aggtcgcagg gaaacgcatc gtcttgaagc
     2341 ctaatctggt cgaattcgat cccaaaggtg tgatcaatac caacccggcg gtcatcgagg
     2401 cggcgaccga tgcgtttcgg acgctgggtg ccgcagaagt gatcgtcgcc gaagggccgg
     2461 gtcaccggcg tgacaatgag tacctgctct ccgcgtccgg actttacgat gttctgcgcg
     2521 aacacaaagt ccgctatgtc gatctcaaca cagatgatat tcgcgccacg ccggtgcgca
     2581 gcagctacac cagcctcgcg cagctgtacc tgcccgagac gctgttcaat gccgatttac
     2641 tggtctccat gcccaaacta aaaacccatc attgggcggg cgtaacgctg tcgatgaaaa
     2701 atttgttcgg cattattcca ggagcagtct atggctggcc gaagaatatt cttcactggc
     2761 agggtatcga caacagcatc ttggatatca atagttcgct gccgctaccg cagtttgcca
     2821 tcgttgacgg catcgtcggg atggaaggca acggtccact gcaaggccaa gctaagagca
     2881 ccggtgtgct gatcttcggc gatgattttg tatcggtcga cgccagcgcg gcaaggctga
     2941 tgaaattaga accgcggaag atcaatcatc tggcgaaggc cgatgcattc ctcggcaact
     3001 tggctaatga gaaaatcgcg caaatcggcg aaagattaga gagccttcag caagacttcg
     3061 agatcatcga cagtttgaaa gatctaaaaa aaatcaccgg ctgaagccaa agggatcgta
     3121 aattgaacac tcgcgacgcc gcatcacttg cgtgccgtga taactccgca ggcggcgcga
     3181 tcgccagcat ttccggtcgg atcggtaacg tgatcatcga tgccgacatg cacgattagg
     3241 gcactgccat ccgcatcgaa aactgaagtt gatccagacc gaagtgtgac ggcgtccgtc
     3301 agcgcttcaa aacggcccga accatccttg gcaaccagca tgttcggcaa gtcgccggcg
     3361 tgggcgccat ctgggctctt gtagccatgt tttttctgcg ctggattaaa atgtccaccg
     3421 gcactaacaa atcctggagt ctcacacttg ccaactgcat ggatatgtac cgcgtgaagg
     3481 ccagcgctga gccctttcac ctccagtcta accctcacgc cgccgcccgc ctcggtaaac
     3541 gacgccacac cgacactctt gccatccttg tccttgagtt cggctgtcgc tcgttcgcca
     3601 gaaccgactg acgaacatcc cacgccgaca acgagggacc aggcacaaat tgacagcatt
     3661 aatatccggg cacgatgggc tttatagttg tggaaatatt gcataggaaa ttcgatacca
     3721 tccattgatg cgcctagcaa tcacatcatt acaatagcag ctcacggtgt acgttactca
     3781 acgtttagcc attgattgcg gcgcattcgg gcctatggta gctttcgacg ttatttatta
     3841 tggccgcgct agcgatatat ctgccaacgg ttgtttttat ttcattgcta ggcggcttcc
     3901 tgccattaat gcgggcgtta tcgcaacggg cactcgcgct gctgctaagc ttcagcgccg
     3961 gcgtcctgct cggcgcggta ttttttcaca tgctgccgga agtgggtagt ctattgggtg
     4021 ccaacctcgg tctgccgatt ttggccggct tcctgctgat tttcgtcatg gaacgtttcg
     4081 tttttgtcca tgcctgcgaa gagcatgatt gcgatatcca tcagatgggc attccggcgt
     4141 ttctcggtat ctccttgcac agcctgctcg acggtgtcgc gctcggcgcc ggactgattc
     4201 tgccgcagct cgggccggtg gtcttgctgg ccgtgattat acacaaggtg cctgacagca
     4261 tgtcgatttc atcgatcctg ctttccgccg gctgggatcg ccacaaagtc ggcaagttga
     4321 atctattgtt ttcgctcacc acaccgatag gggcgttgct ggcatttctc tccttccgcg
     4381 tgctctcgcc ggaaaacgtc gccatcgctg tcgggttgtc cgccggtacc ttcctcgcca
     4441 tcgccacagc ggacatcttg ccgcatgtgc accgcatcga gcagcgcaat ccgatgactc
     4501 tagtcttcct ggcgattggc ttagccgtta gctggatggg cacgctattg gcgcactagg
     4561 caagttgcaa cgagcgatcg cccttctaca ccttatattt gccggcgagc aaattgcgcc
     4621 gcaccgtgaa ggtatcgccg aggacatgaa gatagtccga cattttaacg tggctgcggt
     4681 catcgttgag ccagcgcgcc gggatgatac cgatcttgta attgagcctc tgtgccaaag
     4741 ccaaaacttc gatgtcgaat ccccagcggt caatcgtcgt ctgagaaaaa attttttccg
     4801 cagcctccgc gcggaacgct ttaaagccac actgcgtatc ccaaatcccc ggtaccgccg
     4861 cgagctgcac gatacggttc ccgacctttc cgatggcgcg cttgtgccaa gcctgagaga
     4921 cgacctgcgc cgctcccgca acgtccgccg cattgcgtga tgcgataacg acatcgcagc
     4981 cctgatcgaa gagcgggcgc atctggtcga agtgagcaat atcagttgag ttgtccgcat
     5041 ccgtaaacag ccggatctga ccgcgagctg cgagcatgcc ctccgtgacc gagaaccctt
     5101 tgccgcggtt gtggctgcgg tcaatgattt tgagatgcga aatctcgttg gccatgctcc
     5161 gcagaactac cgccgaagca tcgctcggtc catcaaggac aactataact tcaaatctgt
     5221 atgatttatt cgacaagtac tgctgcaagc gactcagagt agagggcaat ctctccgcct
     5281 cattgtatgc gggaacaatc accgacagat atgcgctatt cgttgacaac tagcagtcca
     5341 gacgcaagaa tccgatgtaa attagcgttt aattcaaagt agactgagag agttcgccat
     5401 tgagcactat cttcccaaga tcacacgcaa gcaaacgaac cccaaaaaga accgatatta
     5461 actccggtat caaatggcaa aattgtttgg ggaattcaga tcgcgattgt atggattgcg
     5521 acatatttta acaagttatt gtgcggccgg agcggaacga agaattcgca gcgcttcgcg
     5581 cagatgtttg gcggcttcgt ccttgttgcc aagttcggac agggcgctcc ccagactctc
     5641 atgcacttcg gcatcctcgg gccggagccg cgcagccttg gtaaaatgat gcactgcttg
     5701 ggtcagatcc ccttgggccg ctacgacacg acctagttgc aaaagaattc gaccatcctc
     5761 cgggtcagcc cttgcggcaa atgtgaatac gtccgtggct tcagccaagc gccccgctat
     5821 cgccaaaact gtaccgagct ttatccgagt gccgtttcgt tggggtgcca actctagggt
     5881 ttttcgaaaa tgctgaaccg cctcatcgat ccggccctga ttcaccaacc ccgcggctag
     5941 attctctcga gcgtcgataa gatcgggttt gaggcgaatt acgtattggt agtgttcgac
     6001 cgctccggca attttacctt gctcctgcag taatcgagca gccgattcgt gagcgtccc
//
LOCUS       DATLCH010000373         3672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6560645, whole genome shotgun sequence.
ACCESSION   DATLCH010000373 DATLCH010000000
VERSION     DATLCH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6560645"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="VMZ02_11185"
     CDS             <1..1096
                     /locus_tag="VMZ02_11185"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HUR72544.1"
                     /translation="VDELRRYHGINQGGAFARMERITRTGFTLSEQILTVETILRIIG
                     LTENFARLVLFCGHASRSENNPFEAALDCGACGGNAGKPNARVIAIMANKPQVRDQLA
                     KNGIHIPQDTYFLAGQHITTTDEIELFDLEDLPPTHRKDLLQLVRDLERAGARNSQER
                     CAYFPEVNKKLRASKARREVRRRSSDWSQVRPEWGLSGNAAFIIARRSLTRGINLEGR
                     VFLHSYDYQGDPDGRLLEAVMTGPQVVGQWINMEHYFSAVDQDVYGSGSKIYHNVVGR
                     LGIMTGPQSDLRTGLAWQTVMNGSRPYHEAMRLLTVIEAPRDRIDKVIRNQRLLQRLY
                     DNEWMQLFALDPQLQLCYRYIPKQGWSGSL"
     gene            1149..1487
                     /locus_tag="VMZ02_11190"
     CDS             1149..1487
                     /locus_tag="VMZ02_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HUR72545.1"
                     /translation="MNDLTLHPMKEIRIIIEGEQLKYATDLLDHKATGYTVIHNISGK
                     GHHGFHTAHPMFNEMDSIVMLMTVVAAENVEPILAGLKPLFAKHTGVMFVSDVAVSRV
                     EYFSTASNDK"
     gene            1631..2500
                     /locus_tag="VMZ02_11195"
     CDS             1631..2500
                     /locus_tag="VMZ02_11195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HUR72546.1"
                     /translation="MSEATLSGLTLHSRGKVRDIYDLGEELLLVASDRISAFDVILPT
                     PIPDKGKILTRLSEFWFSKIGDIVPNHFITTKVDQFPLACQPHRSLLEGRSMLVKKST
                     PAPVECIVRGYLTGSGWKDYQMTGAVCGITLPPGLVEATRLDEPLFTPSTKAAMGDHD
                     VNITFNHMIEKVGKVKAAKMRDASIAIYQRARALAETKGIIIADTKFEFGMEGDDILL
                     IDEVLTPDSSRFWPADEYRAGKTPNSFDKQFVRDYLLSLKWNMQPPAPELPPEIVKKT
                     QDKYTEALRRLTA"
     gene            complement(2489..3070)
                     /locus_tag="VMZ02_11200"
     CDS             complement(2489..3070)
                     /locus_tag="VMZ02_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72547.1"
                     /translation="MPRANRKSQVRVLVYGFGPYRQFKENITAQIIEALAPRPGLRKI
                     IFPVRFSRKQFVDSLRAPMPLMVLGLGQSSRPRIEVETRAINRRRVSKNNAPRPITFG
                     GEAWMPATMPLSVTRPSQKSINAGDYVCNYSMYVMLEEIERQDHYGRFAFVHIPFDHD
                     LKAACLYVERVVGECFEFADKLERERAAKRLGG"
     gene            complement(3142..>3672)
                     /locus_tag="VMZ02_11205"
     CDS             complement(3142..>3672)
                     /locus_tag="VMZ02_11205"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HUR72548.1"
                     /translation="SAIGAAMIGWYGTAMLCYVTPKEHLGLPDKKDVKDGVIAYKIAA
                     HAADLAKGHPGAQERDNALSQARFEFRWEDQFNLSLDPDTAREFHDETLPAQGAKVAH
                     FCSMCGPHFCSMKITQDVRDYAAELGLQEEAALAAGMKEKAEEFKKSGGKIYQEVGVV
                     GADESAPNNMRAAAKV"
BASE COUNT          838 a         1035 c          987 g          812 t
ORIGIN      
        1 cgtcgacgaa ctgcggcgat atcacggtat caatcaaggg ggcgcctttg cacgcatgga
       61 acgcatcacc agaaccggtt ttaccctgag cgagcagata ttgactgtcg aaaccattct
      121 ccggatcatc ggtttgacgg aaaacttcgc caggctggtg ctgttttgcg gtcatgcaag
      181 ccgatccgaa aacaaccctt ttgaagcagc ccttgattgc ggtgcctgcg gcggcaacgc
      241 cgggaaaccc aatgcgcgtg tcattgcgat catggctaac aagccgcagg tgcgggatca
      301 gctcgcgaaa aacggcattc atattccaca agacacctat ttcctcgccg gacaacacat
      361 caccactacc gatgaaatag aacttttcga tctcgaagac ctgccgccga cgcatcgcaa
      421 ggatctcctg cagcttgttc gcgatctgga gcgtgccggc gcgcgcaata gtcaggagcg
      481 ctgtgcgtat tttcccgaag tgaacaaaaa gctgagagct tctaaagctc gccgggaagt
      541 tcgccgccgc agcagcgact ggagtcaagt gcggccggag tggggcctat ccggcaacgc
      601 cgcctttatc atcgcgcgcc gttcgctcac tcgcggcata aatctcgagg gacgggtttt
      661 tctccattcc tacgactatc agggcgatcc ggatggacgg ttgctggaag cggtgatgac
      721 cggccctcaa gtcgtcggtc aatggatcaa catggagcat tatttctcgg ccgtggacca
      781 ggacgtctat ggcagcggca gcaagatcta ccacaatgtg gttggccgtc taggaattat
      841 gacgggtccc caaagcgact tgaggaccgg cctggcttgg cagacggtaa tgaatggctc
      901 gcgcccgtac cacgaagcga tgcgcctgct cacggtaatc gaagcgccac gggaccggat
      961 cgacaaagtg atccgaaatc aacgtctact tcaacgtctc tatgacaacg aatggatgca
     1021 gctttttgct ctcgatcctc aattacagct atgttatcgc tatattccca aacagggatg
     1081 gagcggcagc ctttgagtaa gagctgttct tttcctttgc aagccggccg tccgaaagga
     1141 gatgtaccat gaacgacttg acgcttcatc caatgaagga aattaggatc atcatcgagg
     1201 gcgagcaatt gaaatacgcc accgatttgc tcgaccacaa agcgaccggc tataccgtca
     1261 tccataacat ttccggaaaa gggcaccacg ggtttcatac cgcccacccg atgtttaatg
     1321 agatggacag cattgtcatg ctgatgaccg tagtggcggc ggaaaacgtg gagccgattc
     1381 tcgccggcct taaaccactt tttgccaaac acaccggcgt catgttcgtc tcggatgtcg
     1441 cggtcagccg ggtggaatat ttttccaccg cctccaacga caaatgatcg agtttagctc
     1501 ggctttcttg ctggctgctt gagacttgag gccattcatg gcggaatggc ttaaccgtgc
     1561 tcgcccgtaa cgcgaaaacg tcgaccggtc gcaacgatcg gtgcgcagtt tttctgacca
     1621 aggattgcca atgagtgaag ctaccctttc aggattaacc cttcacagcc gcggtaaggt
     1681 tcgagacatt tatgatcttg gcgaggaact tctgctggtt gccagcgatc gcatttccgc
     1741 tttcgacgtg atcctaccga cgccaattcc cgacaagggc aaaatcctca cgcgactgtc
     1801 ggaattttgg ttcagtaaga tcggcgacat cgtgccgaat cattttatca cgactaaagt
     1861 cgaccagttt cccctcgctt gtcagccgca ccgctcgctt ctcgaggggc gcagcatgct
     1921 ggtcaagaaa agtacgccgg ctccagtcga atgcatcgtc cgcggttacc tcaccggctc
     1981 gggatggaaa gactatcaaa tgaccggtgc ggtgtgtgga attaccctgc cgccgggctt
     2041 agtcgaagcg acgcgtcttg acgaaccgct cttcacccca tcgaccaaag ctgccatggg
     2101 tgatcacgac gtcaacatta cttttaatca catgatcgag aaggtcggca aggtcaaagc
     2161 tgcaaaaatg cgcgacgcta gtattgccat ctatcaaagg gcccgagctc tggccgagac
     2221 caagggcatt atcatcgccg ataccaaatt tgagttcggt atggaaggcg acgatatatt
     2281 gttgatcgac gaagtattga ctcccgattc gtcgcgcttc tggccagcgg atgagtaccg
     2341 cgccggaaaa accccgaaca gctttgacaa acaattcgtc cgcgattacc tgcttagcct
     2401 aaaatggaat atgcaaccgc cggcgccgga attgccgccg gagatcgtca agaaaactca
     2461 agataaatac actgaagcgc tcagacggtt aaccgcctag ccgcttagcg gcgcgttcac
     2521 gttcgagttt gtcggcgaat tcgaaacatt ctccgacgac ccgttcgacg taaaggcaag
     2581 cggccttcaa gtcgtgatcg aaggggatgt ggacgaacgc gaagcggcca taatggtctt
     2641 gccgttcgat ctcctcaagc atcacataca ttgagtaatt acacacgtag tcaccggcat
     2701 tgatcgactt ctgcgaggga cgcgtcaccg agagcggcat ggtcgccggc atccaggctt
     2761 cgccgccgaa ggtgatcggc cgcggcgcgt tatttttact gactcgccgg cgattgatcg
     2821 ctcgggtctc gacttcaatg cgcggccgcg aactctgtcc caaacccaag accatcagcg
     2881 gcatgggcgc tctcaaacta tcgacgaatt gcttacggga aaaccgcacg ggaaaaatta
     2941 tcttcctaag ccccggacga ggcgccaaag cctcgatgat ttgtgcggtg atattttctt
     3001 tgaactggcg atacggccca aagccgtaga ccagcacccg gacctgggat ttacggtttg
     3061 cgcgaggcat gggccaatgc ttaaaaaccg aactggccgc tcatggcggc cagttctttg
     3121 ctaaatcgtt atcgccggcg cttagacctt cgccgccgcg cgcatattat tcggggcact
     3181 ctcgtctgcg ccaacgacac cgacttcttg gtaaatttta ccgccggatt ttttgaactc
     3241 ttcggccttt tccttcatgc ccgcagccag tgcggcttct tcttgaaggc ccaactccgc
     3301 ggcatagtcg cgtacgtctt gggtaatttt catcgagcag aagtgcggcc cacacatgga
     3361 acagaaatgc gccaccttag cgccctgtgc aggcaaggtt tcgtcgtgga actcgcgggc
     3421 tgtgtcgggg tcgaggctga gattgaactg gtcttcccag cggaactcga aacgtgcctg
     3481 gctaagggcg ttgtcgcgct cctgagcacc cggatggccc ttggccaagt cggcagcgtg
     3541 ggcggcgatc ttgtaagcaa tcacgccgtc tttgacgtct tttttatccg gtaagccaag
     3601 atgttctttg ggcgtcacgt agcacagcat cgccgtgccg taccagccga tcattgccgc
     3661 accgatggcg ct
//
LOCUS       DATLCH010000374         5080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6561252, whole genome shotgun sequence.
ACCESSION   DATLCH010000374 DATLCH010000000
VERSION     DATLCH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6561252"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VMZ02_11210"
     CDS             complement(<1..546)
                     /locus_tag="VMZ02_11210"
                     /inference="COORDINATES: protein motif:HMM:NF026164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72549.1"
                     /translation="MKKFQLRIFSVSLIVLLIITTAGLVAGAWFLKDWEDKVTKKFEG
                     RKWDFASKIYSDTYHLYVGINLRLDDLTEKLRRLGYYESSAPPKTQGAYHINAGEGSV
                     DIFLHDFDFPTEKIKGYPVRISLQGNTVARIEVLPSKTEKFDLELEPEVVTGLYQRIW
                     QERRVVKLGEVPPLLVKSILAI"
     gene            complement(663..2165)
                     /locus_tag="VMZ02_11215"
     CDS             complement(663..2165)
                     /locus_tag="VMZ02_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HUR72550.1"
                     /translation="MEPESKWGGSISLKGAVLVALVSLVVGLGISGSLDWLAPGRAVN
                     FLGESAAPESRSSGGLPDFVSLAKKMRPLVVNISTTQVSEGRGGGGGQEFGSPFGEDD
                     QFNDFWRRFFGGPTPRGPQRQRSLGSGFIIDRDGSILTNNHVVENATKIVVKLSDDQE
                     YEAKVIGRDAKTDIAVIKISAKSSLPAANFGDSDNLEVGEWVVAIGNPFGLDSTVTSG
                     IVSAKGRHIGQGPYDNFIQTDASINPGNSGGPLINLRGEIVGINTAIFSRTGGNMGIG
                     FAIPINLVKELLPQLRGKGKVTRGFLGVLIQKVTPEIADSLGLDKAQGALVANVSKDG
                     PADKAGIKVGDVIVEFDGKEVKDSGDLPIIVARTPVDKKVRMKVVREKKEVVLGVAVG
                     ELKDEEVVASAPEKGELGLSVQRLTPQMAESLGLEKADGVVVSAVEPGSAADEAGIRR
                     GDVLMEIDRRPIRNLEEYRKSVAAIKKGRGVLFLVRRGESTLFLALKPQR"
     gene            complement(2174..2605)
                     /locus_tag="VMZ02_11220"
     CDS             complement(2174..2605)
                     /locus_tag="VMZ02_11220"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HUR72551.1"
                     /translation="MADEEKQENKGFTVQDRRRFSPETGEAREDAADSKETASQASSQ
                     AATSDQATAEADRANPLPEIDFSTFVISLSTQALMHLGEIADPISGKIDADITVAKQM
                     IDILGMLKDKTRGNLNSNEDRMIEGILFDLRMKYVEAVKKR"
     gene            complement(2761..3798)
                     /locus_tag="VMZ02_11225"
     CDS             complement(2761..3798)
                     /locus_tag="VMZ02_11225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HUR72552.1"
                     /translation="MNSGEASLGKTDSSRRVCVLFPGALGDFVCFLPALHELARLGAV
                     DLIARSEFAAIAPRAVTVHSLERSEVRRLFTDAGATDPGVQQFFSRYAAVFSWFASDQ
                     LIFLEQLQKVSHGCAHIFPFRPKNSFIHQADYYLSCVDPDVPNAPQPLVALRADALLW
                     CDSFCRRTKIDNRPLLVMAPGSGAREKNWPEANYVQVMRWWREQFAGNVVVVMGPVET
                     ERHRFDQLASSGCIIASELDLAQLASLLSRCNVYVGNDSGVSHLAAAVGARTVVIFGP
                     SDERKWAPRGPRVSIVRRQVACSPCDDTAMKLCPHRACLTELTSAEAIHELRKLPEIA
                     NLTRLRVEITV"
     gene            3877..4830
                     /gene="cysK"
                     /locus_tag="VMZ02_11230"
     CDS             3877..4830
                     /gene="cysK"
                     /locus_tag="VMZ02_11230"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HUR72553.1"
                     /translation="MASLNGARSILDLIGSTPVVKLRSLPGKDDAEVWAKMESFNPGG
                     SVKDRICLNMIETAERQGKIKTGATIIESTSGNTGIGLALVAAVKGYKLVLTMPDTMS
                     EERRSLLAAYGAHLVLTPDTKGMGGAIHKAEELLQENSDFYMPQQFNNPANPDIHRKT
                     TAQELLKQFKRIDAFVAGVGTGGTITGVGEVLKERLNGVRICAVEPASSPVIAGGEPG
                     YHKIQGIGAGFIPAVLNRNILDEVIPVSDEEATQHTRRLAAEEGLLVGISSGAACCAA
                     LKVAQRLGKGHVVVTLFADKGEHYLSTDLFSDKRWTQSSAT"
     gene            4806..>5080
                     /locus_tag="VMZ02_11235"
     CDS             4806..>5080
                     /locus_tag="VMZ02_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72554.1"
                     /translation="MDAKFSHLSEILRQSQQVLVAFSAGVDSTFLLKAAYLALGDGVV
                     ALTASSASVPPGELDAAKSFAAGLGCRHIVLDSHEIDNPAYMANPVNR"
BASE COUNT         1209 a         1472 c         1292 g         1107 t
ORIGIN      
        1 tatggcaaga atcgatttga cgagaagcgg cggaacttcg cccaacttga caacccgccg
       61 ttcttgccaa atccgctgat acaggcccgt gacaacttcc ggctcgagtt ccagatcgaa
      121 cttctcggtc ttgcttggga gcacctcgat gcgagcgacg gtgtttccct ggagcgaaat
      181 ccgcaccgga taacctttaa ttttttccgt ggggaaatcg aaatcgtgga gaaagatgtc
      241 aaccgaacct tcgccagcat tgatatggta tgcgccttgg gtcttcggcg gcgccgaaga
      301 ctcatagtaa cccagccggc gtagcttctc cgtgaggtcg tcgagacgga gattaatccc
      361 gacgtagaga tgataagtat ccgaatagat cttcgaggcg aaatcccatt tgcgcccctc
      421 aaacttcttg gtgaccttat cttcccaatc tttgagaaac catgcccccg cgaccagacc
      481 ggcggtggta atgatcagaa gaacgatgag gctgacactg aagattcgaa gctgaaactt
      541 cttcaaaccc gatgctccca attgaccgtc gaattttcgc gcagattagc agtgaatgca
      601 atgaaaagag ggctggcaaa acgccaagcc ctcgataaaa tttttaacac tggcaaatta
      661 acttaacgct gcggtttgag cgccaagaat aacgtgcttt cgccacggcg caccagaaac
      721 aaaacgcctc tgcctttttt aatcgcggca accgacttgc gatactcctc gagattgcgt
      781 atcggacggc ggtcgatttc catgagcacg tcgccgcggc ggataccggc ctcatcagcg
      841 gcgcttccgg gctctactgc tgaaaccact accccgtcgg ctttctccag tcctaagctc
      901 tcggccattt gaggcgtcag ccgttggacg ctcagcccca gttcgccttt ctcaggtgcg
      961 gatgcgacga cctcctcgtc tttgagttca cccaccgcaa caccgaggac aacttccttt
     1021 ttctcgcgaa ccaccttcat gcggaccttt ttatccacag gggttcgcgc gacgatgatc
     1081 ggcaggtcac ccgagtcttt cacttccttg ccgtcaaact cgacaatgac gtcgccgacc
     1141 ttgataccgg ccttgtctgc tggcccatct ttagaaacat tggcaaccaa tgccccttga
     1201 gctttgtcca agcccaggct gtcggcgatc tccggcgtca ccttttgaat caatacaccc
     1261 aagaagccac gcgtgacctt gcctttgccg cgcagctgcg gcaggagttc ttttacaaga
     1321 ttgatgggga ttgcgaagcc gatccccatg ttgccgccag tccgactgaa aattgcggtg
     1381 ttaatgccga caatctcgcc gcgcagattg atcaagggac cgcccgagtt acccggattg
     1441 atcgaggcgt cggtctgaat aaaattatcg taaggcccct ggccgatatg ccgccctttg
     1501 gcgctaacga tgcctgaggt cacggtgctg tctaggccga agggatttcc gatggcgacg
     1561 acccactcac ccacttcgag attatcggag tcgccaaaat ttgccgccgg caacgacgac
     1621 ttggcgctga tcttgatcac cgcgatgtca gtcttggcgt ctctgccaat aactttcgct
     1681 tcatactcct gatcgtcgga aagtttcacc acgatcttgg tcgcattctc caccacatgg
     1741 ttgttggtta aaatcgaacc atctcgatcg ataataaacc ctgagccaag actgcgttgg
     1801 cgctgcggcc cgcgcggcgt cggtccaccg aagaaacgcc gccaaaaatc attgaactga
     1861 tcgtcttcac caaacggact gccgaactcc tgtccaccac cgccgccgcg accttcgcta
     1921 acctgggtcg tggagatgtt gaccaccaaa ggcctcatct ttttggcaag cgaaacaaaa
     1981 tcgggtaggc cgcccgatga acgtgattca ggcgcggccg attcgccgag gaagttaacc
     2041 gcccggcccg gcgccaacca atcaaggctg ccgctaatgc cgagaccaac aaccagcgag
     2101 accagggcga ccaataccgc gccttttaaa ctgatgctgc caccccactt actttccggt
     2161 tccatccaag ctcctatcgc ttcttcaccg cttcgacata cttcatacgc agatcgaaaa
     2221 ggatgccttc aatcatccga tcttcgttgg aattcaaatt gccacgagtt ttatctttca
     2281 gcatccccaa aatgtcgatc atctgcttgg ccacagtgat atcagcgtca atttttccgc
     2341 tgatcggatc ggcaatttca cccaaatgca tcaacgcttg tgtgctcagg ctaatcacga
     2401 acgttgaaaa atcaatttcc ggcaaagggt tcgctcgatc agcctccgcg gtcgcttggt
     2461 cactggtggc cgcttgcgat gaggcttgac tcgcggtctc tttcgagtcg gccgcgtcct
     2521 cgcgcgcttc cccggtctcg ggcgagaatc gccgccggtc ctgtaccgtg aaacctttgt
     2581 tttcttgctt ttcttcgtcc gccatttcgc tactcccgcc aatccgagcg aaaatcgctt
     2641 gggaaagtga acgcgcgatt aagatccagg cgcggtccgt gagccaatcg gtgtctattt
     2701 ggggttggcc acgacaaaat aaaaatattg cccaactctt cctctcaaac accaccggat
     2761 tcaaactgta atctccaccc gtagccttgt caagttagcg atttctggaa gctttctcag
     2821 ctcatggata gcttctgcag aagttagctc agtgagacaa gcgcgatgcg gacagagttt
     2881 catggcagta tcgtcacacg gcgaacaggc aacctgacgc cgtactatcg acacgcgcgg
     2941 cccgcgcggc gcccatttgc gctcgtccga agggccgaaa atcacgaccg ttcgcgcccc
     3001 aaccgcggcg gcaagatgag aaacaccgct atcattgcca acataaacat tgcagcggct
     3061 aagcaagctc gccaattgcg ccagatcgag ttcactggca ataatacacc ccgacgaagc
     3121 caactgatca aaccgatgcc gttccgtctc gaccggaccc atgacgacta caacgttgcc
     3181 ggcgaactgc tcgcgccacc aacgcatcac ctgaacgtaa ttggcttcgg gccaattttt
     3241 ttctctggcg ccactcccgg gagccatgac cagcaacggt ctgttatcaa tctttgtgcg
     3301 acgacagaaa ctgtcgcacc aaaggagcgc gtcggcacgt aacgcaacca atggctgtgg
     3361 tgcgttcggc acgtctggat cgacacatga aagataataa tccgcttgat ggatgaaaga
     3421 gttttttggt cgaaacggaa aaatatgcgc acaaccgtgt gaaacttttt ggagctgttc
     3481 gagaaaaatt aattggtcgc tggcgaacca agaaaatacc gcggcatacc tcgaaaagaa
     3541 ttgttgaaca ccgggatctg tcgcaccggc atcggtgaac aatcggcgaa cttcggagcg
     3601 ttccaatgag tgcactgtga cagcacgggg agcgatcgct gcgaactcgc tacgggctat
     3661 aagatcgacg gcgccgagac gggcgagttc gtgtaacgcc ggcaagaaac aaacaaagtc
     3721 gccgagagcg ccgggaaata gtacgcagac ccgccgcgac gagtctgttt tgccgagcga
     3781 ggcttcgcca gaattcattg acattaaccg gtcaagtata tagcattctc acaaagccat
     3841 tcgaaatcac tccgatcacg aaagtcgaga taattagtgg caagcctcaa tggcgcgcgg
     3901 tcaatcctag atttgatcgg ctccaccccc gtggttaaac tcagaagcct tcccggtaaa
     3961 gacgacgccg aagtgtgggc gaaaatggag tcattcaatc cgggcggatc ggtcaaagac
     4021 cgcatctgct taaacatgat cgagactgcc gagcgccaag gcaagatcaa aaccggcgca
     4081 accattatcg agtcgacgag cggcaatacc ggaatcggat tggccttggt cgcagcggtt
     4141 aaaggctaca aattagtcct gacgatgccc gacaccatga gcgaggaacg gcgcagtctt
     4201 ttggcggcat acggcgcgca cttggttctt accccagaca ccaaaggcat gggcggcgca
     4261 atacacaagg ctgaggagct gcttcaggag aatagcgatt tctacatgcc ccagcagttt
     4321 aacaacccgg ccaatcccga catacatcgc aaaaccacag cgcaagagtt gctcaaacag
     4381 tttaagcgga tagatgcttt cgtcgccgga gtcggcacag gcggtacgat tactggagtc
     4441 ggcgaagtac tcaaagaaag actcaatggc gtaagaatct gtgccgttga accggcatcg
     4501 tcgccggtta tcgccggcgg cgagcctggc tatcacaaga tacagggcat tggcgccggt
     4561 ttcattccgg cggttttgaa tcgtaatatt ctcgacgaag tgattcccgt cagcgacgag
     4621 gaagccaccc aacatacccg ccgtctagcc gccgaggagg gtctcttggt cggcatttcg
     4681 tcgggcgcag cttgctgtgc agcgttgaag gttgcgcaga gactcggcaa aggccatgtg
     4741 gtagtcaccc tattcgccga caaaggcgag cattacctca gcaccgatct tttcagtgat
     4801 aaacgatgga cgcaaagttc agccacctga gcgaaattct gcgccaatcg cagcaagtct
     4861 tagtagcgtt ctctgccggc gttgattcga cttttttatt gaaagcggcg tatctcgctt
     4921 tgggcgacgg agtcgtcgca ctcaccgcgt catcggcctc ggttccgccc ggagagcttg
     4981 acgcggcaaa aagtttcgct gccggcttag gctgccgcca tatcgttctc gactcccatg
     5041 agatcgacaa tccggcgtat atggcaaatc cggtcaatcg
//
LOCUS       DATLCH010000375         4273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8117543, whole genome shotgun sequence.
ACCESSION   DATLCH010000375 DATLCH010000000
VERSION     DATLCH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8117543"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /gene="hflX"
                     /locus_tag="VMZ02_11240"
     CDS             complement(<1..472)
                     /gene="hflX"
                     /locus_tag="VMZ02_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HUR72555.1"
                     /translation="MPSRDHKVPLDYSLDELERLAETAGALVVHKYSQQVRRVTPATL
                     IGRGKVDEVHSGLQEHHANLVILDEDLSPAQQRNLENALNTRVVDRSQLILDIFAKRA
                     RSNEGKLQVELAQLEYLLPRLTRQWTHLSRLGGGIGTRGPGETQLEVDRRRIRER"
     gene            complement(529..4026)
                     /gene="dnaE"
                     /locus_tag="VMZ02_11245"
     CDS             complement(529..4026)
                     /gene="dnaE"
                     /locus_tag="VMZ02_11245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HUR72556.1"
                     /translation="MDPGKFVHLHCHTEYSLLDGANKVDKMFERIKALEQPAVAMTDH
                     GNMFGAIEFYREAKSRGIKPIIGCEIYVAPTSRFDKKGVDKGPKEYNNHLILLAMNND
                     GYRNLCKLVSLGYIEGFYYKPRIDKEVLRQLNGGLIALSACLQGEVSQALNYGNYEKA
                     KLAVEQYASIFGDRYYIEIQDNKLAEQEKVNRLLVELAKDTSIPVVATNDCHYGERAD
                     YHAHDVLLCVQTGKTLDDDNRLKIETDELYLKSAEEMTQGFGFCPGAVERTLEIAERC
                     NVEIEFGKYHFPNFTPPKEISLDDYLVELAHTGLEERLEGVTDPQVRKTYVERLEYEL
                     GVIIDMQFPGYFLVVADFINYAKQNGIPVGPGRGSSAGSLVAYAMRITDLDPIRHVLL
                     FERFLNPERKSMPDIDVDFCIRGRAQVIQYVKDKYGADRVAQIATFGTLKAKAAIKDV
                     GRALGFSFADADAIAKLIPPPKQGFDYPLSEAMKMEPRLGELMKNDVRVKTLMDHALR
                     LEGLVRHASTHAAGVVLSNLPLVDHLPLFVDKEGGIVTQYEMTWVEKIGLVKFDFLGL
                     KTLTLIHDCLKLIEARGIKIDIDRLPLDDKKTYKTLCQGNTTGVFQLESTGIREMTVR
                     IRPNCFEDLVAILALYRPGPLDSGMAEDFINRKSGKEKIKYLHPLLETILKDTYGVIV
                     YQEQVMQTAQILAGYSMGDADILRRAMGKKDPEEMAAQRERFVSGAVAKNIEEKRATE
                     IFDQMETFARYGFNKSHSAAYALVSYQTAYLKTHYPVEFMAALLTSEMGDTDKVIKNL
                     ADCRDKNIEVLAPDVNESGSNFTPVGDKIRFGLAGVKNVGEKAVEVILETRNQDGPFA
                     SLFDFCRRVDMTAVNRRVIESLIKCGAFDSTQVSRARMSGALDDAMKAGQGYQRDQAS
                     NQIDIFSMLGTTTKGSKKAGESYPQVTEWSQQEALAFEKEALGFYITGHPLDKYERLL
                     KKITSGTVTALKERGQSGEFRLGGVVSALRLRNTKKGDRYGSFNLEDKTGFIEVIVWP
                     ESYKKCADLLGADDPIYVRGKMEVGEDRIQLIANEVTALAEAVKNPKNASASAVAEKV
                     DLYVREDAVSADELVRLRDMLLDYPGRHTVYLHLRGSAVAETVIELPEQVRIAPSREL
                     EALVGERFGMRVSFHSLNN"
     gene            complement(4072..>4273)
                     /locus_tag="VMZ02_11250"
     CDS             complement(4072..>4273)
                     /locus_tag="VMZ02_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72557.1"
                     /translation="KFIYYAEIDRYAADLTLYEGQRLAAIAAAERSDYKFANILAGVV
                     EDFAAAGLWPRTVDNFPDIKLV"
BASE COUNT          921 a         1193 c         1139 g         1020 t
ORIGIN      
        1 tgcgctcgcg aatgcgccgg cggtcgacct ccaattgcgt ctcacccggc ccgcgggtac
       61 caatgccgcc gcccaagcgc gacaagtggg tccactggcg cgtcaatcga ggcaacaagt
      121 attccaactg cgccaactcc acctgcagct tgccttcgtt gctgcgcgcc cgcttagcga
      181 aaatatcaag gatcaactga ctgcgatcga ccacacgggt gtttagagcg ttttccagat
      241 tgcgctgttg cgccgggctg agatcctcgt cgaggatcac caagttggcg tgatgttcct
      301 gaagtccaga gtggacctcg tctaccttgc cacggccaat cagcgtcgcc ggcgtaacgc
      361 gacgaacctg ttgcgaatat ttgtggacca ccaaagcacc ggcggtctcg gcaagccgct
      421 cgagttcgtc caggctgtaa tcgagcggca ctttgtggtc gcgtgacggc atatcgacgc
      481 cgatcaaaac ggccctttcc tgcggctgtc gggtagaaat cgtttgcttc aattgtttaa
      541 ggaatggaaa ctgacgcgca tgccgaagcg ttcaccgacc agagcctcga gctcacgtga
      601 tggcgcgatg cgcacttgtt cgggcaactc aatgacggtt tcagcgacgg ccgagccgcg
      661 caaatgcaaa tagacggtgt gcctacctgg atagtcaagc agcatgtcgc gcaaacgcac
      721 caattcgtcg gcggacactg cgtcttcgcg cacatacaga tcgactttct cagcgactgc
      781 gctggcgctg gcgttcttag gatttttcac ggcctcagct aaagcggtaa cttcgttggc
      841 gatcaattgg atgcggtctt caccaacctc catcttacct ctaacgtaga tcggatcgtc
      901 ggcgccgagc agatcggcgc atttcttgta agactcgggc cagacgatta cttcgataaa
      961 gccggtcttg tcctctaaat tgaaactgcc gtagcggtca ccttttttag tattgcgcaa
     1021 ccgcaaagct gaaaccacgc cgccgaggcg gaactcgccg gactggccgc gctctttgag
     1081 cgccgtaacc gtgccgctgg taatcttttt cagcaaccgt tcgtatttgt cgaggggatg
     1141 accggtgatg taaaaaccca aagcttcctt ctcgaatgcc agcgcctcct gctgcgacca
     1201 ctcggtaacc tgcggataac tttcgccggc tttcttcgac cccttggtgg tcgtgccgag
     1261 catggaaaaa atgtcgatct gattgctcgc ctgatcgcgc tggtatcctt gccctgcctt
     1321 catggcgtcg tccagtgcgc cgctcatgcg cgcccgcgac acttgcgtcg agtcgaaggc
     1381 accgcatttg atcaaactct cgatcacccg acggtttacc gcggtcatgt cgacgcgccg
     1441 gcaaaaatcg aacagtgatg cgaatgggcc atcctggtta cgagtttcga gaatcacttc
     1501 gacggctttc tcaccgacat ttttgacacc ggccaaacca aaacgaattt tgtcaccgac
     1561 cggcgtgaaa ttggagccgc tctcgttgac gtcgggcgcc agcacctcga tatttttatc
     1621 gcgacagtcg gcgaggtttt tgattacctt gtcggtgtcg cccatttccg aggtcaacag
     1681 cgcagccatg aactcgacgg gataatgagt tttgaggtaa gccgtctgat aggaaaccag
     1741 cgcataggcc gcagagtgcg atttgttaaa accgtagcgc gcgaatgttt ccatttgatc
     1801 gaagatttcg gtggcgcgct tctcctcgat atttttggcc accgcgccgc tgacgaagcg
     1861 ctcgcgctgc gccgccatct cttcgggatc cttcttgccc atcgcgcggc gcagaatatc
     1921 ggcatcgccc atcgaatagc cggcgagaat ctgcgctgtc tgcatgacct gctcttgata
     1981 gacgatcacg ccataggtgt ctttgagaat ggtttcgagc agcggatgga gatatttaat
     2041 cttttctttg ccgcttttac gattaataaa atcttcggcc atgccgctat cgaggggacc
     2101 ggggcggtag agggcgagga tagcgaccag gtcttcgaaa cagttaggtc gaatgcgcac
     2161 agtcatctcg cgaatgccgg tgctttccag ctggaacacg cctgtggtgt tgccttggca
     2221 gagggtttta taggttttct tgtcatccaa tggcagacgg tcgatatcta ttttaattcc
     2281 gcgagcctcg atgagcttca aacaatcgtg aatcagcgtg agagtcttga gaccgagaaa
     2341 atcaaacttc accagaccga ttttttcgac ccaggtcatt tcatactgag tgacgatgcc
     2401 gccttcctta tcgacgaata gcggcaagtg atcgaccagc ggcaaattcg acagcacgac
     2461 gccggcagcg tgagtcgagg catgacgcac caagccctcc aaacgcagcg catggtccat
     2521 taaggttttc acgcgcacgt cgttcttcat caactcgccg agccgcggtt ccatcttcat
     2581 cgcctcgctt agcggataat cgaaaccttg cttgggcggc ggaatcagtt tggcgatggc
     2641 atcggcgtcg gcaaatgaga agcccaaggc ccggccgacg tctttgatgg ctgccttggc
     2701 tttgagcgtt ccgaaagtgg cgatctgtgc cacccggtcg gcgccgtatt tgtctttgac
     2761 gtattgaatg acctgcgccc gaccacggat gcaaaaatcg acgtcgatat cgggcatgga
     2821 tttgcgttcc ggattgagaa aacgctcgaa cagcaaaaca tggcgaatcg gatccaggtc
     2881 cgtaatgcgc atggcgtagg ccaccagact ccctgccgac gaaccgcggc caggaccgac
     2941 gggtatacca ttttgcttag cgtaattgat aaaatcggca acgaccagaa agtagccggg
     3001 gaactgcatg tcgatgatta cgcccagttc gtactccagg cgctcgacat aagttttgcg
     3061 aacttgcggg tcagtgacgc cttcgagacg ttcctccagc cctgtatgcg cgagctcgac
     3121 caagtagtcg tcaaggctga tctctttggg cggcgtaaag ttggggaaat ggtacttacc
     3181 gaactcgatt tccacattgc agcgctcggc gatttccagc gtgcgttcca cggcccctgg
     3241 acaaaacccg aatccctgcg tcatctcttc ggcggatttt aaataaagtt cgtcggtttc
     3301 gatcttcaag cggttatcgt catcgagggt cttaccggtt tgcacacata gcaagacatc
     3361 atgcgcatga tagtcggcgc gctcgccata atggcaatcg ttggtcgcta cgaccggtat
     3421 cgatgtatcc ttggcgagct cgaccagcag tcgattgact ttctcttgct cggccaattt
     3481 gttgtcttgg atctcaatgt agtagcggtc accgaatatc gacgcatact gctcgacggc
     3541 aagtttggct ttttcatagt tgccgtagtt gagcgcctgc gatacttcgc cttggagaca
     3601 cgcgctcagc gcaatcagtc cgccgtttaa ttgtcgcagc acttccttat cgatgcgcgg
     3661 cttatagtag aacccttcga tataccccaa ggacaccagc ttgcacaggt tgcggtatcc
     3721 gtcattgttc atggccaaga gaatcaaatg gttgttatat tctttgggcc ctttatcgac
     3781 acctttctta tcgaaacgac tggtcggcgc gacgtaaatt tcgcaaccaa tgatcggttt
     3841 gatgccgcgg ctcttggcct cgcgataaaa ttcgatggca ccgaacatgt tgccatgatc
     3901 ggtcatcgcc accgccggct gttcgagcgc cttgatgcgc tcgaacatct tatcgacctt
     3961 attggcgccg tctaagagac tgtattcggt gtgacagtgt aggtgaacga atttgcctgg
     4021 atccatcgcg gtggaaaaat ttctatcaca atcggaccgg cgcgacaaat ctcagacgag
     4081 ttttatgtcg ggaaagttat ccacagttcg cggccacaat ccggcagcgg caaaatcttc
     4141 gaccactccg gcgagaatat tggcgaactt gtagtcggac cgttcagctg ccgcgatggc
     4201 cgctaatcgc tgaccctcat ataaagtcaa gtccgcagcg taacggtcga tctcggcata
     4261 ataaatgaac ttg
//
LOCUS       DATLCH010000376        10520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10348812, whole genome shotgun sequence.
ACCESSION   DATLCH010000376 DATLCH010000000
VERSION     DATLCH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10348812"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VMZ02_11255"
     CDS             complement(<1..862)
                     /locus_tag="VMZ02_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HUR72558.1"
                     /translation="MLNMATRKAMLRQMVLVRAFEEKLEELYQRKAMYGSTHSYRGQE
                     AIAVGVCSALQPNDLIASYHRGTGHLIAKGADLYTLLCECMGREEGYSKGRGGKMHMG
                     DMAFGFIGNTGTVGGTVPTATGAALSAKIRGSQEVVASFFGDGAANQGVVHEAMNLAG
                     VWKLPVIYICENNQYAMTVSLAKSVAVKQLSVRGAAYGFEGKTVDGNDVEAVYEAMQQ
                     AAAKARSGGGATFLECVTFRWDGHFGGDPGTAYRTKEEIESWKQKCPIKRLKEKFIAA
                     GDLTEVEFQKM"
     gene            complement(1038..2522)
                     /locus_tag="VMZ02_11260"
     CDS             complement(1038..2522)
                     /locus_tag="VMZ02_11260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HUR72559.1"
                     /translation="MDLKNVASLTFFCPEIILSATILLLIVLDLIASSRRWPAALALI
                     GCVCSLIVSFDLYSAEPGMLFHRMIVLDNFSIFFKVFSLAATILCILMSIGSNEIERV
                     HEAEYYALLLTCALGMFFMASSSNLLMAYLSLEMVSLGSYVLTGFLPHSRRSSEAALK
                     YLIYGGVASGTMIYGMSWIFGMTGSLDYSAIQLALSQEGISKATVFIAFVFILAGFGY
                     KIVFFPFHMWSPDVYQGAPTPFTAFLSVASNAAGLAIMIRFFFPGMSKVMASGDWAFT
                     AGVEWPQVLLALSMLTMTVGNLCALNQRNVKRMLAYSGIAHAGYMMMGLAILNNEGLA
                     AILFYTVVYLIMNVGAFLVVGMISNVTGDEDIADYRGLAWRGATVPAVCLTIFLFSLT
                     GLPPFAGFMGKFFLFSAVIRQGGAFAVLAVVAALNSVISLYYYAKLVKTMFLDSPTAE
                     DKQITLAANNYTLLLPLTFLTVLFGVYFGPLVQYTHQSLRFFVK"
     gene            complement(2525..4042)
                     /locus_tag="VMZ02_11265"
     CDS             complement(2525..4042)
                     /locus_tag="VMZ02_11265"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HUR72560.1"
                     /translation="MQNILTYMTFIPLAGAAVILALPNNAKLIRWFSAIVTVPPLLMA
                     IWLFINFDRTTAKFQFVEDYHWISAYNISYTMGVDGLSISMVLLTALLSFLCIFASFG
                     IEKAVKGYFALFLMLDAGMMGVFCALDFFLFYVFWEVMLLPMYFLIGIWGGPRREYAA
                     IKFFLYTLFGSVLMLLAILALYFTVEPHTFDMRILMAQASKYSTAVLPLWPFNLWGWG
                     FQHVIWMALFIGFAIKIPAFPFHTWLPDAHVEAPTAISVILAGVLLKMGTYGILRVNF
                     PMMPVATADLAYWFLGALGAWNIIYGALCAMAQKDLKKLVAYSSVSHMGYVMLGMASM
                     TTQGLNGAVLQMFNHGTITGMLFLLVGVVYDRAHHREIEGFGGLAAIMPVYTGATALA
                     FFASMGLPGLSGFISEILVLLGTWQKYPVLTILGASGVILTAGYLLWTIQRVYLGPPN
                     EKYLNMAEINGREIFTLVPLGAIVIVVGVYPGVVLDMLRTTLDSINAIVIPHLHG"
     gene            complement(4065..6107)
                     /gene="nuoL"
                     /locus_tag="VMZ02_11270"
     CDS             complement(4065..6107)
                     /gene="nuoL"
                     /locus_tag="VMZ02_11270"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HUR72561.1"
                     /translation="MENPIQTDFIRWIVFLPLLGAIVNGLLGAKIQKKFGKSVIGWVA
                     CIPVIVAFVLSVNAFLTLKGLEPEKRFIIDNLYRWIDLGSLKVDMAFLVDPLSSVMIL
                     IVTGIGGLIHIYATGYMHDDKAFWRFFAYLNLFTAAMLTLVLGDSLLLLFVGWEGVGL
                     CSYALIGFWYHDHANARAGNKAFIVNRVGDFGFVLGMFLLFWSLDAQGHGSLTFREMV
                     KWAPSIANMSLWGYSVVTLAAFFLFVGATGKSAQIPLFVWLPDAMAGPTPVSALIHAA
                     TMVTAGVYMTARMNVFFSMAPGVMQLIAFIGVATALVAASIALTQNDIKKVLAYSTVS
                     QLGYMFIGVGVGGYAAAVFHLMTHAFFKACLFLGAGSVIHAMHHEQDMRKMGGLKQWM
                     PITFATFFVSVLAIAGFPPFAGFFSKDEILWLAFTSDHGNKVVWFLALCGAGMTAFYM
                     FRQLFMTFYGKCRADHHTQEHLHESPSVMTMPLVVLAIGAVFAGLIGLPGVLGGSQFA
                     HWLEPVIHAHAEGHGSHALEWGLMAVSVSVAAFGVFIAYLMYLRGSLSPDTFVNLAGG
                     LPYRLFDRKWYFDEIYQAIFVNGTLMLARFWSAFDAYIVDGIVNGVAALTRFAAWLNG
                     LFDTYIIDGLVNAVADVTYWAGNRFRRLQTGNINGYLYGIIIAMAVAVYVKIRYWT"
     gene            complement(6148..6459)
                     /gene="nuoK"
                     /locus_tag="VMZ02_11275"
     CDS             complement(6148..6459)
                     /gene="nuoK"
                     /locus_tag="VMZ02_11275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HUR72562.1"
                     /translation="MQPSELSSYLIIGAILFALGLFTVLTRRNAISVLMGVELILNSA
                     NINYVAFSHFGSGNSAGQIYAIFVIMLAAAEAAVGLAIVLAIFKLFHTIDVEAAETMK
                     D"
     gene            complement(6459..6959)
                     /locus_tag="VMZ02_11280"
     CDS             complement(6459..6959)
                     /locus_tag="VMZ02_11280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HUR72563.1"
                     /translation="MSVSTAVFYLVALLTIVSAGMVAFSRNIIYSAFSLLGTFMGVAG
                     LYIFLGADFVAAVQLLIYVGGILVLILFAVMLTHRITDVAITNRAAGRIPALIVTGGL
                     LYMLIQTVKTTPWIKAKEIVFAATTAKIGDAFLYEYLLPFELASLVLLAAMIGAVVLS
                     RKEIKE"
     gene            complement(7081..7641)
                     /locus_tag="VMZ02_11285"
     CDS             complement(7081..7641)
                     /locus_tag="VMZ02_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HUR72564.1"
                     /translation="MSVASYIKDIRDAITSTFEGMSITFSHLMRRPMTIQYPDKIPVP
                     IQETLPLRYRGILEVDLDICTGCLACERVCPITCITIGIDMNKDTKQRQFAHFDIDVC
                     KCMYCGLCSEVCPTGAIRHSQEFEGASYNPAGLVRHFAPEPSNLYKPKKGGETEPTLV
                     AKVDIGQKYLDEFATPDGGGAAEEEA"
     gene            complement(7655..8845)
                     /gene="nuoH"
                     /locus_tag="VMZ02_11290"
     CDS             complement(7655..8845)
                     /gene="nuoH"
                     /locus_tag="VMZ02_11290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HUR72565.1"
                     /translation="MQSLLDQMMQEGAFAGVPKEVVYAIAMVLTALVVLSVFVAPLAG
                     LTSWLERRVWARMQSRVGPNRVGPQGVLQWLADGIKNIMKEDIVPAAADQKLFPLAPY
                     IVFMGFLCTFVVIPFGENLIVADLDIGILYILAVTSLVVVGILMAGWASNNKWSLLGG
                     MRSAAQIVSYEIPAGLALLTVVFLSGTLSIQGIIKAQGWAPWDWFLFHNPFTFCAFFL
                     YFTAALAEGNRTPFDIPEAESELVAGYVTEYSGMRFLFFFFAEWGNLYVIGAVATSLF
                     MGGWQVPAVFDDHPVAKGIAQFIVFFLKAYSWVFVAMWIRATLPRVRVDQLMALCWKY
                     MVPLSFVCLMGTIGVMFMSNQWQWVFRAVTLGFAIATAINFFRRVGFQLTEAKPEIYL
                     KPHI"
     gene            complement(8863..10002)
                     /locus_tag="VMZ02_11295"
     CDS             complement(8863..10002)
                     /locus_tag="VMZ02_11295"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HUR72566.1"
                     /translation="MSENAATDTGFYAEEMTINVGPQHPSTHGVLRFVVKTDGEMINE
                     AIPDVGYLHRSIEKIGEKVTYHGYVPYTDRADYLAAMFANQVFCMAAEKLGNTEVARR
                     GEFCRVIACELNRIASHLISVGTFGQDIGAMTPFVHALRERETINNLMEEICGARLTY
                     NYIRIGGVGYDIVPSTMAKIAEFLDHFEPIVEEFNRLLSGNKIFIERLAGVAVVTKEE
                     ALSYNLVGPNLRGSGIKWDIRRDIPYSIYPELDFDIPVGTAEVGSVGDSFDRFWVRIR
                     EMQESVKILRQCIKMIPKGPAISKVPRKFRPPAGEYYARVEAPRGDMGFYVISDGSEY
                     PYRVRIRTGSFTAMSIIDKLSKGIMVADLIALIGSLDVDAPEIDR"
     gene            complement(10012..>10520)
                     /locus_tag="VMZ02_11300"
     CDS             complement(10012..>10520)
                     /locus_tag="VMZ02_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HUR72567.1"
                     /translation="MQAKETYASLEKLFPGKVRDFKEDVLDPYFNVEKENIVEICRFL
                     RTAQSFRYEVLSDLTAVDWPKEEKIQLVYHLFSYAHNHQIVLKVDLPRDNPKIATVEE
                     QWKVANWFEREVYDLFGVIFEGHSDLRRIMLPEDWVGFPLRKDYVEQEEYDGISTQRA
                     PLVEKLLR"
BASE COUNT         2779 a         2939 c         2745 g         2057 t
ORIGIN      
        1 acattttctg aaattctacc tcggtcagat cgcccgcagc gataaatttt tctttcagcc
       61 gcttgatcgg acatttttgc ttccacgatt cgatttcctc cttggtgcgg taggcggttc
      121 ctggatcacc gccaaaatga ccgtcccagc gaaaagttac gcattcaagg aatgtcgcac
      181 cgccgccgct gcgtgctttc gccgcggctt gctgcatcgc ttcgtacacg gcttccacat
      241 cgttgccatc gacggtcttg ccctcgaatc cataagcggc accgcgcacg gagagttgtt
      301 tcaccgcgac gctcttcgcc aaactgacgg tcatcgcgta ctgattgttc tcgcaaatat
      361 agataaccgg cagcttccaa acaccggcga gattcatcgc ctcatggaca accccctggt
      421 tggccgcacc gtcgccaaag aagctggcga cgacttcttg cgagccgcgg attttcgccg
      481 atagtgccgc gcctgtggcg gtgggcactg tgccgccgac ggttccggtg ttgccgataa
      541 aaccgaaagc catatcaccc atgtgcatct tgccgccgcg tcccttggag tagccctcct
      601 cgcggcccat gcactcgcac aaaagcgtgt acaggtcggc gcccttggcg attaaatgtc
      661 cggtgccgcg gtgatagcta gcgatcaaat cgttgggctg caacgccgaa cagactccca
      721 cggcgatggc ttcttggccg cgataagagt gcgtgctgcc atacatcgcc ttgcgctggt
      781 aaagctcttc gagtttttct tcgaatgccc gcaccaatac catctgccgc agcattgcct
      841 tgcgtgtcgc catgtttaac ataactccct caacgattta tcaatcaatc gcgaatagcg
      901 aactgctact ttgttatttg cccctggcag agttgtcaag aagtggtgct gatccgcgcc
      961 tgcgtcaaaa atggcggctt atgcggcggc aaaagctgca gcattaagcc gaatcgtttt
     1021 tgaacacgag ttttcgatta cttaacgaag aagcgcagcg actgatgggt gtactgcacc
     1081 aatggtccga agtacacgcc gaagagaact gttaaaaagg tcagcggcaa cagcaacgta
     1141 tagttgttcg ccgccaaagt aatttgctta tcttcggccg tcggggagtc gagaaacatg
     1201 gtcttgacta atttggcgta ataatagagc gagatgacgc tgttaagcgc agcgaccacg
     1261 gcgaggaccg cgaaggcacc gccctgccga atcaccgccg aaaacaagaa gaacttgccc
     1321 atgaagccgg cgaacggcgg cagcccggtc agcgaaaaaa gaaaaatcgt tagacaaacc
     1381 gccggaacag tggcgccgcg ccacgctagt ccacggtagt ccgcaatatc ttcgtcgccg
     1441 gtaacgttag agatcatgcc taccacaagg aaggcaccga cgttcattat caagtaaacc
     1501 actgtgtaga acaggatcgc cgctagcccc tcgttattta gaatcgccag gcccatcatc
     1561 atatatcccg catgcgcaat gccggagtag gcgagcatgc gcttaacgtt gcgctggttg
     1621 agagcgcaga gattgccgac ggtcatggtg agcatgctta atgcaagcag gacctgtggc
     1681 cattccaccc cagctgtaaa agcccaatca cccgaggcca taactttgga catgccgggg
     1741 aaaaagaagc ggatcatgat cgccaaccca gcagcattgg atgccactga aaggaacgcg
     1801 gtgaatgggg tcggcgcgcc ctgataaaca tcgggcgacc acatgtgaaa cgggaagaaa
     1861 acgattttgt aaccgaagcc cgccaggata aaaacaaacg caatgaacac cgtcgccttg
     1921 ctgattcctt cctggcttaa ggccagctgt atagccgagt aatccaagct gccggtcatg
     1981 ccgaagatcc agctcatgcc gtagatcatc gtgccggagg cgacgccgcc atagattaaa
     2041 tacttcagcg ccgcctcgct ggaacgtcgg ctgtggggca ggaacccggt cagcacatag
     2101 gagcccagac tgaccatttc gagggacaaa taggccatta acagattgct cgacgaagcc
     2161 atgaaaaaca tgccgagcgc gcaagtcagc agtagagcgt agtactcggc ttcatgaacc
     2221 cgctcaatct cgttactgcc gatggacatg aggatacata ggatcgtcgc cgccaaagaa
     2281 aacactttga agaagatgct gaagttgtcg agcacaatca tgcggtgaaa cagcatgccg
     2341 ggttcggcgc tataaagatc gaaggagacg atgagcgagc acacgcagcc gatcagtgcc
     2401 agtgccgcgg gccaacgccg gcttgatgcg atcaagtcca gcacgatgag cagcaagatc
     2461 gtcgcggaga gaataatctc cggacaaaag aaagtaaggc tcgcgacgtt cttcagatcc
     2521 atggctagcc gtgcaggtgc gggatcacga ttgcattgat tgagtcaagc gttgtgcgca
     2581 gcatgtcgag caccacgccg ggataaactc cgacgacgat gacaatcgcc cccaatggca
     2641 ccagagtaaa aatctcccgg ccattgattt ccgccatatt gagatatttc tcattgggcg
     2701 gtcctaggta gacgcgctga atcgtccaga gcaaataacc ggcggtgagg atgacgccgc
     2761 tggcgccgag aatcgtcaac accggatact tttgccaggt gccgagcagg acgaggattt
     2821 ccgaaatgaa accagagagc cccggcagcc ccatggatgc gaagaaagcc aacgccgtcg
     2881 cgcccgtgta aaccggcatg atcgccgcga gcccgccgaa gccttcaatt tcacggtgat
     2941 gcgcgcggtc atagaccacc ccaaccaaga ggaacaacat gccggtgatg gtgccgtgat
     3001 taaacatctg caacaccgcg ccattcaatc cctgtgtggt catgctcgcc atgccgagca
     3061 tgacgtaacc catatgactg acgctggagt aggcgaccag tttttttaag tctttctgcg
     3121 ccatggcgca gagcgctccg tagataatat tccaagcgcc gagggcgccg agaaaccagt
     3181 aggccaagtc cgcggtcgcc accggcatca tgggaaaatt gacccgcagg atgccgtagg
     3241 tgcccatctt caataatacg ccggcaagaa ttaccgagat cgcagtaggc gcttcaacgt
     3301 gagcatcggg cagccacgta tgaaatggaa aggccgggat cttgatcgcg aaaccgataa
     3361 aaagcgccat ccagatgaca tgctgaaaac cccaacccca tagattgaac ggccagagcg
     3421 gtaacactgc agtgctgtac ttactcgcct gggccatgag aattcgcatg tcgaaagtat
     3481 gcggctcgac ggtgaaatag agcgcgagga ttgcaagcag catcaacacg ctgccgaaca
     3541 aggtgtagag gaaaaacttg atcgccgcat actcgcgccg cggcccaccc caaatgccga
     3601 tgagaaaata catcggcaac agcatgactt cccagaacac gtagaacaga aaaaaatcca
     3661 aggcgcagaa cacccccatc atgccggcgt cgagcataag aaatagcgcg aagtagccct
     3721 tcaccgcttt ttcaatgccg aaggaagcaa aaatgcagag gaagctcagt aacgcagtga
     3781 gcagcaccat ggaaatactc aatccgtcca cgcccatggt gtagctaatg ttgtaggcag
     3841 agatccaatg gtaatcttca acgaactgaa atttcgccgt cgtccggtca aagttgatga
     3901 acagccagat cgccatcaac agcggcggca cggtgacgat ggcggagaac cagcgaatca
     3961 gtttggcgtt attgggcaac gccaaaataa ccgccgctcc ggccagcgga ataaacgtca
     4021 tgtaagtaag gatattttgc atcgtcccta tgctccagtg aaagtcacgt ccagtatcgg
     4081 atctttacat acacggcgac cgccatcgcg atgatgatcc cgtagaggta accgttgata
     4141 ttgccggtct gcaggcggcg aaaacggtta cccgcccaat aggtcacatc ggccaccgcg
     4201 ttgaccaaac cgtcgataat atacgtatcg aataagccat tgagccaagc agcgaatcgg
     4261 gtcaaagcgg ccacgccatt gacgatgccg tcaacaatat aagcatcgaa ggccgaccag
     4321 aaacgcgcca gcatcaatgt gccattgaca aatatcgctt gataaatctc gtcgaaatac
     4381 catttacgat cgaatagacg atacggcaaa ccgccggcga gattgacaaa ggtgtcgggc
     4441 gacaatgacc cgcgaaggta catgagatac gcgatgaaga caccgaaagc tgccactgaa
     4501 accgaaaccg ccatcaggcc ccactccaaa gcatgcgacc catgcccttc ggcatgagcg
     4561 tgaatgaccg gttccagcca gtgggcgaac tggctgccgc ccaacacgcc aggcaagccg
     4621 atcagaccgg cgaacaccgc gccaatcgcc aataccacca aaggcatggt catcaccgaa
     4681 ggcgattcat gcaaatgctc ctgggtatga tgatcggcgc gacacttgcc atagaaggtc
     4741 atgaataact ggcggaacat gtagaaagcg gtcatgccgg cgccgcacag cgccaagaac
     4801 caaacgacct tgttgccatg atcgctggta aacgccagcc aaagaatttc gtcttttgag
     4861 aaaaatccgg caaacggcgg gaagccggca atcgccagta ccgagacaaa aaaagtcgca
     4921 aaggtaatcg gcatccactg cttcaagccg cccatcttac gcatgtcctg ctcatgatgc
     4981 atggcgtgaa tcacgctgcc ggcacccaag aacaagcagg ctttgaagaa cgcgtgagtc
     5041 attaaatgaa acaccgccgc cgcatagcca ccgacgccga cgccgatgaa catgtagccc
     5101 aattggctga ccgtggaata ggccaaaact ttcttgatgt cgttttgggt caaggcgatg
     5161 ctcgcggcga ccaatgcggt cgccacaccg ataaacgcga tgagctgcat gacacccggc
     5221 gccatggaga aaaagacatt cattcgcgca gtcatgtaaa cgccggcggt caccatggtc
     5281 gcagcgtgga tcaacgcgct caccggcgtc ggcccggcca tcgcgtcagg gagccagacg
     5341 aaaagaggaa tctgcgccga tttgccggtg gcgccgacga aaagaaaaaa cgccgccaag
     5401 gtaacgacgg aatagcccca caagctcatg ttggcgatcg acggcgccca cttgaccatt
     5461 tcgcgaaacg tcaaggatcc atggccttgc gcgtcgagcg accaaaacag caaaaacatc
     5521 cctaggacaa agccaaaatc gccgacccgg ttgacgatga aagctttatt gccggcgcgc
     5581 gcgttcgcat gatcgtgata ccagaaaccg atgagtgcgt aagaacacag cccgacacct
     5641 tcccacccga cgaataacaa aagcaagctg tcgcccaaaa ccaaagtcag catcgccgcg
     5701 gtaaacaaat tgagataggc gaaaaaacgc cagaacgcct tgtcgtcatg catgtacccg
     5761 gtcgcataga tatgaatgag cccgcctatg ccggtgacga taagaatcat cactgaagac
     5821 agcgggtcga cgaggaatgc catatctact ttgagcgagc ccagatcgat ccagcgataa
     5881 aggttatcga tgataaagcg tttttccggt tccaatcctt tgagcgtgag aaaggcgttg
     5941 acggagagca caaaggcaac gatcactggg atacaagcga cccacccgat gacgctctta
     6001 ccgaactttt tttgaatctt cgcgccaagc aaaccattga cgatggcgcc aagcagaggc
     6061 aggaaaacga tccagcgtat aaaatccgtt tgtatgggat tttccatcga gtgttacaac
     6121 cttggagttg gcatttcgac cgttgagcta atccttcatc gtctcggcgg cttcgacatc
     6181 gatggtgtgg aacagtttga aaatagccaa tacgatcgcc aagccgaccg ccgcctcggc
     6241 cgccgctagc atgatgacaa agatcgcgta aatctggccg gcgctattgc cgctgccaaa
     6301 atgggaaaat gcgacataat tgatattggc gctgttcaga ataagttcta cgcccatcaa
     6361 cacgctgatg gcattgcgcc gagttaacac cgtgaataag ccgagggcga aaagaatcgc
     6421 gccgatgatc aaatacgagg agagctcact cggctgcatt actctttgat ctccttgcgc
     6481 gataacacca cggcaccgat cattgccgcc aacaagacca gagaagcaag ctcaaatggc
     6541 aacaaatact cgtaaagaaa tgcgtcgccg atttttgccg tggtggctgc gaaaacgatt
     6601 tccttggcct tgatccaagg agtcgtcttg accgtttgga tgagcatgta aagcaaaccg
     6661 ccggttacga tgagtgccgg aatgcggccg gccgcgcgat tggtgatcgc cacatcggtg
     6721 attcggtgcg tcagcatgac cgcaaacagg atcaacacca gaatgccgcc gacatagatc
     6781 agcaattgca ccgcggcgac gaagtcggcg ccgaggaaaa tatacagccc agccactccc
     6841 atgaaggtgc caagcaaaga gaacgccgag tagataatat tacgcgaaaa agccaccatg
     6901 ccggccgaca ctatggtaag cagcgcaact aaataaaata ccgccgttga tacactcata
     6961 ccctgctcag tttctgagat cgcggaaaaa ttgcagacgt tttcgctgac ttatcggaag
     7021 atccttaacg gatcaagggg gcattgaatg gaaatggaaa ttccgccccg ctccagacgc
     7081 ctatgcttcc tcctcggcgg cccctcctcc atccggcgta gcaaattcgt ccagatattt
     7141 ttgaccgata tcgactttgg caaccaacgt gggctcggtt tcgccgccct tcttgggttt
     7201 gtagagattg gacggctcgg gagcgaagtg gcggaccagg cctgccgggt tatagcttgc
     7261 gccttcgaac tcttgagagt gacggatcgc gccggtcggg cagacctccg aacacagacc
     7321 gcagtacatg catttgcaca catcgatatc gaagtgagca aactggcgct gcttggtgtc
     7381 cttgttcatg tcgatgccaa tggtaatgca agtgatcgga cagacacgct cacaggccaa
     7441 acagccggtg catatatcga gatcgacttc gaggataccg cgatatcgca acggcagtgt
     7501 ttcctggatc ggtaccggga tcttatccgg atattggata gtcatcggcc ggcgcatcaa
     7561 atgcgagaag gtaatggaca tcccttcaaa ggtcgacgtg atcgcatccc tgatatcttt
     7621 tatatagctg gcaacactca tagttcctca acagctaaat atgcggtttg agataaatct
     7681 ccggcttggc ttcggtcaac tgaaaaccga cgcgacggaa aaaatttatc gccgtcgcga
     7741 tggcaaagcc cagcgtcacc gctcgaaaaa cccactgcca ttggttggac atgaacataa
     7801 cgccgatggt gcccatcaaa caaacgaacg acagcggcac catgtacttc cagcacaacg
     7861 ccatcaattg gtcgacgcgc acacgcggca aggtcgcgcg gatccacatg gccacgaaca
     7921 cccaggaata agctttcaaa aaaaacacta tgaactgagc gatgccttta gccacgggat
     7981 gatcatcaaa gaccgccggc acttgccagc cgcccatgaa cagcgacgtg gccaccgcgc
     8041 cgatgacgta aagattgccc cactcggcaa agaagaaaaa cagaaagcgc atgccgctat
     8101 attcggtgac gtaaccggcg accaattccg actcagcctc cggaatatcg aagggcgtac
     8161 ggttaccttc ggccaacgcg gcggtaaagt agagaaaaaa ggcgcagaaa gtgaacggat
     8221 tatggaacag aaaccagtcc cagggcgccc acccttgggc cttgatgatc ccctgtatgc
     8281 tcagtgtgcc ggaaagaaac accacggtta acagcgccaa acctgcaggg atttcgtagc
     8341 tgacgatctg cgctgccgag cgcatgccgc caagcagcga ccatttgtta ttcgacgccc
     8401 acccggccat gagaatgccg actactacga gcgaagtaac ggcgagtata taaagaatgc
     8461 cgatgtcgag atcggcgacg atcaggtttt caccgaaggg aataaccacg aatgtgcaaa
     8521 gaaaacccat gaagacgata tacggggcga gcggaaatag tttctgatcc gccgctgccg
     8581 gcacgatatc ttccttcatg atgttcttaa tgccgtcggc gagccattga aggacgcctt
     8641 gaggcccaac ccgattgggg ccgacgcgcg actgcatgcg tgcccagaca cgccgttcga
     8701 gccagctcgt caagccggca agcggcgcga cgaaaactga caggaccacc agcgccgtga
     8761 gcaccatagc gatggcatag acgacctctt taggaacgcc ggcgaacgca ccttcttgca
     8821 tcatttggtc gagcagggat tgcatagtgt tatcgaaaat atctaacgat cgatctccgg
     8881 cgcatcgaca tcgagactgc cgatcagcgc gatcaaatcc gccaccataa tccccttgct
     8941 aagcttgtcg atgatactca tggccgtgaa cgagccggtg cgaatgcgca ctcggtaggg
     9001 atactcgctg ccatcgctaa taacgtaaaa acccatatcg ccgcgtggcg cttccacccg
     9061 agcgtaatat tcgcccgcgg gcggccggaa tttcctcgga actttggaaa tcgccggacc
     9121 cttgggaatc atttttatgc actgccggag aattttgacg ctctcctgca tctcacggat
     9181 ccgtacccag aaacggtcga agctgtcgcc gacgctgccg acttcggcag tgccgacagg
     9241 gatatcaaaa tccaattccg gatagatcga gtacggaata tcgcgacgaa tatcccattt
     9301 gataccggag ccgcgcaggt taggccccac taggttatac gacagcgcct cttctttagt
     9361 caccaccgca acaccggcca gacgctcgat gaaaatctta ttgcccgaca gcaaacggtt
     9421 aaactcctcg acgatgggtt cgaagtgatc gaggaattcc gctattttcg ccatggtcga
     9481 tggcacaatg tcataaccca ccccgccgat gcggatatag ttgtaggtca aacgcgcgcc
     9541 gcagatctct tccatcaaat tgttgatggt ttcgcgctcg cgcaaggcat gaacgaacgg
     9601 cgtcatggcg ccgatgtcct ggccaaaagt gccgacggaa atcagatggc tcgctatgcg
     9661 attgagttca caggcgatga cccggcaaaa ttccccgcgg cgcgccactt ccgtattgcc
     9721 aagcttctca gcagccatgc agaagacttg attggcgaac attgccgcca aataatcggc
     9781 ccgatcggta taaggcacgt aaccgtgata cgtcaccttc tcgccgatct tctcgatgga
     9841 acggtgcaag tagccgacat ccggaattgc ttcgttgatc atctcaccgt cggtcttgac
     9901 gacgaaacgc aacacgccat gagtgctggg gtgctgcggc ccaacattga tggtcatctc
     9961 ttcggcgtaa aagccggtat ccgtagcggc gttctcgctc atcgctaaac cctatcgcaa
    10021 caatttttcg accaacggcg cacgctgggt gctaatgcca tcatattctt cctgctcgac
    10081 ataatctttg cgcagcggga agccgaccca atcctcgggc aacataatcc ggcgcagatc
    10141 gctgtggcct tcgaagatta ccccgaagag atcgtaaacc tcgcgctcga accaattggc
    10201 gaccttccat tgctcttcga cggtagcgat tttaggattg tcgcgcggca aatcgacttt
    10261 aaggacgatt tgatggttat gagcataaga aaacagatga tagacgagtt gaattttttc
    10321 ttccttcggc cagtcgactg cggtcaaatc cgacagcact tcgtatctaa aactctgagc
    10381 ggtgcgcagg aaacggcaga tttcgacgat attctccttc tcgacattga agtacgggtc
    10441 caggacatcc tctttgaagt cgcggacttt gccgggaaag agtttttcga gtgaagcgta
    10501 tgtttcttta gcttgcatgg
//
LOCUS       DATLCH010000377         7087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10358025, whole genome shotgun sequence.
ACCESSION   DATLCH010000377 DATLCH010000000
VERSION     DATLCH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10358025"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..671)
                     /locus_tag="VMZ02_11305"
     CDS             complement(231..671)
                     /locus_tag="VMZ02_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR72568.1"
                     /translation="MEKITKITKVDVVNEAQWGKPTGRNYARPTQTDRLHIALNSYEP
                     GINDELHCHPGSDHTFFVVQGECTMRGLHDGEEYHLKQHQAVHVPPGFFYQLNNTGTE
                     RLILYQVSTEPLKKPKIGKVIYGVHTGLKEHLYNPVKSENKAAE"
     gene            1085..1246
                     /locus_tag="VMZ02_11310"
     CDS             1085..1246
                     /locus_tag="VMZ02_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72569.1"
                     /translation="MELVPVNAEGAAVSSNAPHPHAALLFIDFLLCPEGQKMYAAKLF
                     YTSATRKVS"
     gene            complement(1776..2612)
                     /locus_tag="VMZ02_11315"
     CDS             complement(1776..2612)
                     /locus_tag="VMZ02_11315"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HUR72570.1"
                     /translation="MSRAERVEEATAEFEALLNYLADTTETPESYGGVQQAEADQKRK
                     GKYAEFPMPIYNGRAIADRLSLEREGFLLVQHDTQVKNFYDGAEVRSIYYKETEELVK
                     RTAGAKRVLVFDHTLRAADSAIREANQVSGPVRNAHNDYTEWSGPQRVRDLLPDEAEE
                     LISRRFAVVQTWRPINKPVQNDHLAIADAQTIGMTELIPSARIYPERRGEVYHLTHSP
                     AHRWYYFPNMQRTEAMVFKCYDSVKDGRARWTAHCAFDDPTAPPHANSRESIEMRTLA
                     FF"
     gene            complement(2729..3367)
                     /locus_tag="VMZ02_11320"
     CDS             complement(2729..3367)
                     /locus_tag="VMZ02_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72571.1"
                     /translation="METQTAAAAQARTSDENAKDRFIRKTRELFAKETDLDKRWNGLR
                     PILAELLADPDMIAASKTWPDCVPANGRAENLLFYEDPDYGFAINGLTKGQARQGVAA
                     RIHDHAHIYTLYGVIDGREKVVRYDRLDDRSKPNFAQIKESANVLCGPGDIDLVKPYE
                     IHTELTVGERTVAVIIRSQKGGDFNQGRYNPETNEYFESLGPRQTPCEMMPK"
     gene            complement(3376..4554)
                     /locus_tag="VMZ02_11325"
     CDS             complement(3376..4554)
                     /locus_tag="VMZ02_11325"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HUR72572.1"
                     /translation="MAKKGFKVFDSDMHIMEPPDLWERYIDKEFLDQAPRGRTSDNVR
                     DLGTIFPKVPKDTRRTGGTPHRGNNFNKNQEIYRNHAERGWTGHVQLEAMNTEGIDTA
                     VLFPTRGLGILTFPDQDPRFADAMARAYNDWLHDFCSADPERLLGAGMISVYNLEDAL
                     RETRRVKEEYGFRSVFLRSNIINGINWYDPHYEPLWDLLEELNMPLGFHEASASRSRQ
                     AAEHFEPNFGLRRVYAQPFEQMMGMGAFLGGGILERHPKMRVAFLEANCSWLPWLLWR
                     LDEGYEREGDVFMPDLKMTPTEYFKRQCWISIEPDEAPARYTIAEFGCDQLVFSTDYP
                     HGDSKYPHAVESFLELTLSDDDKRKILWDNCARFYGAAEYETRKENDRNPTTINATLN
                     "
     gene            4932..5909
                     /locus_tag="VMZ02_11330"
     CDS             4932..5909
                     /locus_tag="VMZ02_11330"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72573.1"
                     /translation="MIFRISVSIVIIFISTVELGAQGRKVRVAMPGYTIAGISFLTAK
                     LNGYYAAEGLDVELIAMRAPTANLAVLSGSVEFSTVPLVGLNTALRGGALKIIMVHFD
                     KPQHVLFVKNDLQNLKSLRGKKIAVSGPGVIDDVLLREVLSNNGIDANKDLAILSMGS
                     ADTRVTSLISGAVDGAMIIAPHTFSAKDAGFRELVVFRDQGVLLPSGGIAVRDDTIKN
                     DPALVDRFTRASMMGFIHSRDNRAGAIRVLSKTLRIEEPVAVKVYDSSRPTMTADGTL
                     SEDAQKKMASFVAKSSGLKEIVTLDKAFDFSFVRKANATLQAKSWQPVQ"
     gene            5958..>7087
                     /locus_tag="VMZ02_11335"
     CDS             5958..>7087
                     /locus_tag="VMZ02_11335"
                     /inference="COORDINATES: protein motif:HMM:NF020896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HUR72574.1"
                     /translation="MRLTLAYHPVTSIHFGSRLNLDGAALTVGQDELRAIVLEDPLIE
                     AVDFEIAAPNDSLRAGPVFDIVEPRAKTTGSSPDWPGILGAPQTAGSGTTHVLTGAAV
                     TMLREDSSGDSRGDTGYVLEMSGAPAAGSHYGTLQHLIIVPHTRLNVPAAARQRAYRL
                     AQLKIAVRLAQTAITIAPQRTEVFDNNDAETRAGLPRLVYIGQIFSRQRKPQADEHIL
                     YGADTDGMLPTLLHPNEWLDGALVPSYHASIGGAETYFYQNHPVILELYRRHRAGEIN
                     FVGSVATIASANNFDRERNTRFAANLTKWALRADAAVLTKFGGGVPHTDLSETARLLE
                     TMGVKTAVQVTDAARDHKVESALLFNFPEVDAIVCIGGNDTH"
BASE COUNT         1505 a         1882 c         2010 g         1690 t
ORIGIN      
        1 gtcgacggtc attttgccgg cgaccataag ttccatgtcg gagccgctgg tgtagtgact
       61 ccagtcaccg atctcaaggt cccagtgcat gactttgctc atagggggta ggttagctgg
      121 gagttgttgg tcgttatagg agatcgggat ctccatgcca cgacccaacg cgcgcacgtt
      181 ggccttctgt tttttgcacc agtcgagtac ttcggcaaac gtcattattg ctactccgct
      241 gctttgtttt ccgacttaac cggattatat aggtgttctt tcaatccggt atgcacgcca
      301 tagatcactt tgccgatctt cggttttttc aagggctcgg tgctcacctg gtacaggatc
      361 aatcgctccg tgcccgtgtt gttgagttgg tagaagaaac ctggagggac atggacggct
      421 tggtgttgct ttaggtgata ctcttcgccg tcatgcagtc ctctcatggt gcactcgccc
      481 tgaaccacga aaaaggtgtg gtcggagccg gggtggcagt gcagttcgtc gtttatgccg
      541 ggctcgtagc tgttgagcgc tatatggagc cggtcggtct gcgtcggtcg cgcgtagttg
      601 cggccggttg gcttgcccca ttgagcctca ttcacgacgt cgactttggt aatctttgta
      661 atcttttcca tggatatcct cctggtaact tcaatcccac caaatctaca ggatgttggc
      721 gccgaaacac aagtgccggc gcaactggtg attccccgcg tcgacagaat tgaacgccga
      781 caatagactc ctagtgtgca atgtcgtcac tcacgctgat agctcgccta aaacgatccg
      841 gctggctaga aggaacctgt ttcgattgac aaaggttggc atgaagagat tcgttactgt
      901 ttttattatg ccgcatcaga gcgcctatcc cgagatagcc aaggaaaggc cgaaggcgat
      961 ctggtgtggt ggagcacgga ccgcgagtcg ttgaggcgtc ggatataaca aaaacacctt
     1021 tcctctgccg atctgccgaa gcgcatcgat gatcttctca agccagccag ttgggtgggg
     1081 tacgatggag ctcgtcccgg tcaatgccga aggagccgcg gtatcgtcca acgcgccgca
     1141 tccgcacgcg gcgttactat tcattgattt tctactttgc cccgaaggtc aaaagatgta
     1201 tgccgctaaa cttttctata ccagcgcgac tagaaaagtc agctaggaaa agtggtatcc
     1261 ggagaaaggg gcttactacg gcggagtatg aagaacgcgc tgacaagtgg ctcaaactct
     1321 tgcgcgaaat ccgccgaaag tagctctggc gctccatttc gggtggtttt atgatttaaa
     1381 attatgttaa ttaacaaatg cttgccgata cttccgagtg ttctcttgag gattaccaag
     1441 ctaagttgat cggttcatcc cgtgtaatat tttcaagccg gtgaaattca aaggaaaata
     1501 cctctgcagt ggggtaaatc ccgcgccccc tctctttgac cgccaattga ccagtgataa
     1561 tcgtacctgc tccagttaag ggggagggca taatggctgc aaaaatcgac tgtatgacat
     1621 gccggaaacg gccaagaaaa aagacccgga aatagacgat ccaaatcgaa gatagtaggt
     1681 agaagatcga acaactctcc gtacggacat cacaaacaaa aaggacctgc gattaaacaa
     1741 cgcaggtcct ttttgtttgt gcctgtttgc aaattttaga aaaaagccaa cgtgcgcatt
     1801 tcgatgcttt cgcgcgaatt cgcgtgcggc ggggccgtcg gatcgtcgaa ggcgcagtga
     1861 gcggtccaac gagcgcgccc atctttcact gaatcatagc atttgaatac catggcttcg
     1921 gtgcgctgca tattagggaa ataataccac cggtgagcag ggctatgggt cagatgatac
     1981 acttcgccgc gcctttcggg gtatatgcgt gcgctgggga tcaattcagt catgccgatc
     2041 gtttgggcat cggcgatggc caggtgatcg ttttgcaccg gcttattgat cggccgccag
     2101 gtctgcacga ccgcgaagcg ccggctgatg agttcttcgg cttcatccgg caataagtca
     2161 cgcacgcgct gtggtccgga ccattctgtg taatcgttgt gcgcgttgcg caccgggccg
     2221 ctcacctgat tggcttcgcg gatggcgctg tcggcagcgc gcagggtgtg atcgaatacc
     2281 aacacccgtt tggcgccggc tgttcgcttt acgagttctt cggtttcttt gtagtagatt
     2341 gaccgtactt cagcgccgtc atagaagttt ttcacctggg tatcatgctg taccagaagg
     2401 aatccttcgc gttccagcga aagccgatcg gcgatggcac ggccgttgta tatcggcatc
     2461 gggaattcag cgtacttgcc cttgcgcttt tgatccgcct cggcctgctg cacaccgccg
     2521 taactctccg gggtctcggt agtgtcggcg agataattca gcagtgcttc aaactctgcc
     2581 gtggcctctt cgactctttc agctcgtgac ataaaggatc cttcgaagac gcgaacagtg
     2641 ctcaccacac agacgcgaag gttgaacaaa actgtactcc cggtcgtggt gaaaaatctc
     2701 tctcatctca tctcggctcg agcgctcgct actttggcat catttcgcag ggcgtctgcc
     2761 gtgggcccag gctttcgaaa tattcgttgg tctcgggatt gtagcggccc tggttaaaat
     2821 caccgccttt ttggctgcgg atgattaccg cgacggtgcg ctcgccgacc gtgagctccg
     2881 tgtgaatttc ataaggctta actaaatcta tatcgccagg tccgcagagc acgttggctg
     2941 attcctttat ctgtgcgaag ttgggcttcg aacgatcgtc aagccgatcg tagcgcacaa
     3001 ctttctcgcg gccgtcgatg acgccataga gtgtgtagat atgcgcatgg tcatggatcc
     3061 gcgcggcgac accctggcgc gcttggcctt tggtgaggcc gttaatggcg aaaccgtaat
     3121 cggggtcttc gtagaacagc aagttttcgg cgcggccgtt ggccggaacg caatctggcc
     3181 atgtcttcga cgcggcgatc atatcaggat cggccaaaag ctcggccagg attggccgga
     3241 ggccgttcca gcgtttgtcg agatctgttt ctttggcgaa cagctcgcga gtcttcctga
     3301 tgaaacgatc cttggcgttt tcgtcgctag ttcgtgcttg cgctgcggcg gcggtttggg
     3361 tttccatgtc tcctcctaat ttaaggttgc attgattgtt gttgggttgc gatcgttttc
     3421 tttgcgggtt tcgtattctg ccgcgccgta aaagcgcgca cagttgtccc acagtatttt
     3481 gcgtttgtcg tcgtccgaaa gtgttagttc gagaaaactt tcgacggcgt gcggatactt
     3541 cgagtcgccg tgcggatagt cggtggaaaa taccaattga tcgcagccga actcggcgat
     3601 tgtatagcgc gccggagcct cgtcgggttc gatggagatc cagcactggc gtttgaagta
     3661 ctcagtcgga gtcattttca aatctggcat gaagacatcg ccttcgcgtt cgtagccttc
     3721 gtctaaacgc cacaacagcc aaggcagcca ggaacagttg gcttcgagaa acgccacgcg
     3781 catcttgggg tgacgctcca aaattccacc gccgaggaaa gcgcccatgc ccatcatctg
     3841 ctcgaacggt tgggcataaa cccggcgcag gccgaaattg ggctcgaaat gttccgccgc
     3901 ttggcgcgaa cgcgacgccg aggcttcatg aaagcccagc ggcatgttca attcctcgag
     3961 caaatcccac aaaggctcgt agtgcgggtc gtaccagttt ataccattga tgatattcga
     4021 gcgcagaaac accgaacgaa aaccatactc ttctttgacc cggcgagtct cgcgcaaagc
     4081 atcctcgaga ttgtaaaccg agatcatgcc ggcgccgagc agccgctcgg gatcggcgga
     4141 acaaaaatca tgcagccaat cgttataggc gcgtgccatc gcgtcggcga agcgcggatc
     4201 ttggtcggga aaagtgagga tgcccaagcc gcgcgtcgga aatagcacgg cggtgtcgat
     4261 gccttcagtg ttcatcgctt ccaactgcac atgcccggtc cagccacgct cggcatgatt
     4321 ccggtatatt tcctggttct tattgaaatt gttgccgcga tgcggcgttc cgccggtgcg
     4381 ccgggtatct tttgggacct taggaaagat cgtaccgaga tcgcgaacat tgtcagaagt
     4441 cctgccgcgc ggcgcctgat caagaaattc tttatcgata tagcgttccc aaagatcggg
     4501 cggctccatg atgtgcatgt cgctgtcgaa gactttgaat ccctttttcg ccatgaagta
     4561 cccccttaga tttttcgagc gtaatgctga cccagggcgg atgtcaagca aggataaggc
     4621 gagattctga ccgcaatttt tttgctttgg gtgttggtcc gtcgacttcg cgagatgcgg
     4681 gcatattaat cgcggcgcta ggaattactc tctgattgct tacgtgtgat aggaaagact
     4741 gcaccaggtt acgtgtgcct tttaggcggc ccgcgagcac tttgcgagca cacttatgag
     4801 atggtgcggc tgagcaacgc aaggctgatt cctagatggc aaattctttc tgtgtcaatt
     4861 gacaagcgct ctcggcccaa ttacatctca ggcagttgac gcgcaaaatg gcgtcaccgg
     4921 gagctatcag gatgattttt cgtatatcgg tcagtatcgt tattattttc atcagcaccg
     4981 tcgagctcgg cgcgcaaggc cgcaaggtgc gggtggcgat gccgggttac accatcgccg
     5041 ggatttcgtt tctgacggcg aagctcaatg gttactacgc cgccgaaggg ttggatgtgg
     5101 agctgatcgc catgcgcgcg ccgacggcga atctcgccgt gctgagcggc agcgtcgagt
     5161 tctcgacggt gcctttggtg ggactcaata ccgcgcttcg cggcggtgcg ctgaagatta
     5221 tcatggtcca cttcgacaaa ccgcagcatg tgctgttcgt caaaaacgat ttacagaacc
     5281 tgaaatcgtt acgcggcaaa aaaatcgccg tgtccggtcc gggggtgatc gacgatgtgt
     5341 tgttgcgcga agtgttgagc aacaacggca tcgatgccaa caaggatctc gccattctca
     5401 gcatgggatc ggcagatact cgcgtgacca gtttgattag cggcgcagtc gacggcgcga
     5461 tgatcatcgc accgcatacg tttagcgcca aagatgcagg cttccgcgaa ctagtagtgt
     5521 ttcgcgacca aggcgtgctc ttgccgagcg gcgggatcgc agtgcgggat gacacaatca
     5581 aaaacgatcc ggcgctggtc gatcgcttta cccgagcgtc gatgatgggc ttcatacatt
     5641 cgcgcgataa tcgcgccggc gcgatccgtg ttctgagcaa aaccttgagg atcgaggaac
     5701 cggtcgctgt gaaagtttac gattcctcgc gcccgacaat gactgcggac ggtacgctca
     5761 gcgaagacgc gcaaaagaag atggctagct tcgtcgctaa atcgtcaggg ctcaaagaaa
     5821 ttgttacgct ggacaaggca ttcgatttct cgttcgtgcg caaggccaat gcgacgcttc
     5881 aggccaagag ttggcagccg gtacagtgaa ttgacgacgg tgctcgagcg gcattaaagt
     5941 ttctctggaa gagcaatatg cgactgacat tggcttatca cccggtaact tcgattcact
     6001 tcgggtctag attgaatttg gacggcgctg cgctgacggt tggtcaagac gagttacgcg
     6061 ccatcgttct cgaggacccg ctgatcgaag cagtcgactt tgagattgcc gcgcccaacg
     6121 atagcctgcg cgccgggccg gtattcgaca tcgttgagcc tcgcgccaaa accacaggct
     6181 cgagcccgga ttggccgggg attctcggcg cgccgcaaac cgcgggcagc ggcactaccc
     6241 atgttctcac cggcgcggca gtgaccatgc tgcgcgaaga ctcctcgggc gactcgcggg
     6301 gtgatactgg ctatgtcctt gaaatgagcg gagcgccggc ggcgggttcg cactacggga
     6361 cgctgcagca tttgattatc gtgccccata cccgtctgaa tgttccggct gccgcgcgcc
     6421 agcgtgccta tcgtttagcg cagttgaaaa tcgccgtgcg attggcacag acggcaatta
     6481 ctatcgctcc gcaacgtacc gaagtcttcg ataataatga tgccgaaacg cgcgccggct
     6541 tgccgcggct cgtctatatc ggccaaatct tttcgcggca gcgcaagcct caagccgacg
     6601 aacatattct ttacggtgcc gataccgacg gcatgctgcc cacgttattg catcccaatg
     6661 agtggctcga tggcgctctg gttccgtcgt atcatgcgtc gatcggcggt gctgaaactt
     6721 acttctatca gaatcatccg gtgattctgg aactctacag gcggcatcgt gcgggtgaga
     6781 taaatttcgt cggctcggtg gcaaccatcg catcggcaaa caatttcgac cgcgagcgca
     6841 acacccgctt tgcggcaaat ctgacaaaat gggcgctgcg cgcggatgcc gcggtactga
     6901 ccaagttcgg cggcggcgtg ccgcacacgg atttgtcgga gaccgcgcgc ttgctcgaga
     6961 ccatgggcgt gaagacggct gtgcaagtga ccgacgcggc gcgcgatcat aaagtcgagt
     7021 ccgcgctctt gttcaacttt cccgaagtcg atgccatcgt ttgcatcggc ggcaacgaca
     7081 ctcactg
//
LOCUS       DATLCH010000378         5299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8145734, whole genome shotgun sequence.
ACCESSION   DATLCH010000378 DATLCH010000000
VERSION     DATLCH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8145734"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VMZ02_11340"
     CDS             complement(<1..774)
                     /locus_tag="VMZ02_11340"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72575.1"
                     /translation="MSINGKAIRIVGAKQNNLKNLDIEIPLNKLTVVTGVSGSGKSTL
                     AFDILYAEGQRRYVESFSAYARQFLDRMDKPDVESIEGIPPTIAIDQSRPVRTSRSTV
                     GTMTELHDHFKLLFAKIAVLHCRGCQRVVERDSAPSVMQKLNSLAEAAPVVVTFPLAA
                     SSVSWEDVRNGLSQAGFHRLLLDRTICELDEIERPPAKNQTLQVVVDRFAFRADNKKR
                     IIDSLEQAFHYGKGRLNLYLQHEEWRCEPFSNHLHCAHCD"
     gene            1110..1796
                     /locus_tag="VMZ02_11345"
     CDS             1110..1796
                     /locus_tag="VMZ02_11345"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HUR72576.1"
                     /translation="MRISEIFYSIQGEGRLIGVPSVFVRTSGCNLRCRWCDTPYTSWK
                     PEGKSWSIKNILCEVARYPTRHVVITGGEPLLAPDIQDLTVQLKQRGAHITVETAATI
                     FKPLAGDLISLSPKLANSTPWQRSRGAFAAMHERRRLNYQVMQSFIDRYDYQLKFVVD
                     RKKDLEEVRAVIGQLQNIDVSKVMLMAQGIRHEELVRKSQWIVASCQQYGFSYTPRLH
                     IQLFGNRRGT"
     gene            complement(1870..4992)
                     /locus_tag="VMZ02_11350"
     CDS             complement(1870..4992)
                     /locus_tag="VMZ02_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HUR72577.1"
                     /translation="MSGKSLPTELLPGPSLAGRLLFFVNFKRLKVWLWWLAAMAILAA
                     CLFVEIQTSVLQSWFFTSTNKRLSYTLKDGPSREIIFPRAAPFDERRGYSKLPGLQTR
                     LQSQGYQVTKQTRQSETMLTLLERGVSPPYREGPGAGLEIRGADGVQLFRYAQADFVF
                     GKIDDIPSLLVKTLLFLENRDLDRPATPWQNPVIEWDRSFKAALYYLGSKLQLPVSVQ
                     GGSTLAVQLEKFRHSPHGRTDDPFEKLRQIIGASLKAYQEGPNTRAWRERIIVDYLNT
                     VPLAAAPAYGEIHGLGEGLYAWFGIRLSDAVKDLKSDKFTPAKVHAFKHALTLLISVR
                     APTVFLVDERASLEEKVNQFTRLMARAGIIDWEFAARLQETPIKFLPTAPLPPQPSSI
                     KNKAANAIRVSMMEVLGVNNLYDLNRLHLQVDSTIDVPLQKRVSDFFNHLGQREVIKA
                     YGLDGERLLESNDPSKMTYSFLLVEATPTGNLVRVQADNLAASLDFNKSVKLELGSTA
                     KLRTLTHYLELVAELHKELSGLEPKELQERGYAARDPLSKWAAETLRTDNAMALQPFL
                     DKAMQRKYSGSPYEAFFTGGGLHHFENFDKKENERIFELRDALRNSNNLAFIRLMRDL
                     VSYHRARLAYDANDVLDNPDNPTRRQMLQEIADEESRVALRRAYQTYAKQTPDEIVKR
                     ILGTKRNIERRLAVLFFAWKIGNDEEALTAWLEKHEAKSAETDVTKLFRAYHNPRLTL
                     VDYAYLLSMHPLDLWCASEYRKNGELTWEALYHKSVKARHLGSAWLLNPRNQRAQLNR
                     LRIRIEKDAFLRMAPYWQRLGFPFKSLVPTYATAIGSSSDRPTALAELVGILVNDGIR
                     RPATTLTKIHFASNTPYETLLEKQQNQGEAVLAPEIAHTMRKAMAEVVEQGTARRLSG
                     VFKLADGTVLTVGGKTGSGDNRFETFNRSGGVISSRATNRTATLVFYIGERYYGVLTA
                     FVQGQEAANYHFTSSLPVNLLRLLAPSIISKFDNKNLDQVAPPVRSTPAHVTTKSISV
                     PPTDRQSATN"
BASE COUNT         1159 a         1363 c         1559 g         1218 t
ORIGIN      
        1 atcgcagtgg gcgcaatgga ggtggttgct gaacggctcg caacgccact cttcgtgctg
       61 caagtacaaa ttcaatcggc ccttgccgta gtgaaacgct tgctccaaag aatcgataat
      121 gcgctttttg ttgtcggcgc gaaaagcgaa acggtcgaca accacttgca gtgtttgatt
      181 cttagcggga ggtctttcga tctcgtccag ctcacaaata gtccgatcaa gcagtaatcg
      241 gtgaaagccg gcctggctaa gaccgttgcg aacatcttcc caactcaccg acgatgcggc
      301 caaggggaag gtgacgacga ccggtgcggc ttcggccaga gaattgagtt tttgcatgac
      361 tgacggcgcg ctgtcgcgtt caacgacacg ctggcagccg cgacagtgaa gcacggcgat
      421 cttggcgaac aaaagtttaa aatgatcatg cagctcggtc atggtgccga cggtagagcg
      481 cgaggtgcgc accggccggc tctgatcgat ggcaatggtc ggcgggatgc cctcgatgct
      541 ctcgacatcc ggcttgtcca tacgatcgag gaactgccgg gcgtaggcgg aaaaactttc
      601 gacatagcgg cgctggcctt cggcgtagag gatatcgaag gcaagcgtcg acttgccgga
      661 accgctgacg ccagtgacca ccgtgagctt gttgagcgga atctcgatat cgagattctt
      721 cagattgttc tgtttagctc cgactatgcg gatcgctttt ccgtttatgg acatcgatgg
      781 ctattgattg gtttcgctcg cgaccgagta atgtcacgat tatttcgtgg ctggccaatg
      841 cgtcacgctt aatgtgtttt gagcaccgcg gaaaccgacc cggtgcggcg gtttatgaac
      901 tcgaacttcg cgctgttcgc ctcctattgt ggcgaaaaga tttacgcaag tacgcccaag
      961 cgaagctcag cgcagcccgt gccgcatccc tgcttattga tagttgaatt gatcgggcgc
     1021 caaccccgta cgtctatcat agagtcccga cgaatcaagg tcgatttccc gtgctttcat
     1081 ggagttaacg tgtctgttag tttcagcgca tgcgcatcag cgagattttc tactcgattc
     1141 agggcgaggg ccgcttaatc ggcgtgcctt cggtgtttgt ccgcaccagc ggctgtaatt
     1201 tgcgctgccg ttggtgcgac acgccttaca catcgtggaa acctgagggc aaaagctgga
     1261 gcatcaaaaa catcctgtgt gaagttgcca gatatccaac ccgccatgtg gttatcaccg
     1321 gtggtgaacc cttgctggcg ccggacatcc aagacttaac ggtgcagctt aaacaacgcg
     1381 gcgcgcatat caccgtcgag accgcggcga cgatcttcaa accgctggcc ggagatttaa
     1441 tttcgttgag tcctaagctc gccaattcga cgccatggca aagaagcagg ggtgcgtttg
     1501 ccgcgatgca cgaaaggcgc cggctcaatt accaagtcat gcaaagcttt attgacaggt
     1561 acgactacca gctaaaattt gtcgtcgatc gaaaaaaaga tctcgaagaa gtgcgcgctg
     1621 tgatcgggca gttacaaaac atcgatgtat caaaggtcat gctgatggcg cagggaatca
     1681 ggcacgagga actggttcgc aaatctcaat ggattgtcgc gtcatgccag cagtatggtt
     1741 ttagttatac cccgcgcttg catatccagc tgttcggcaa tcgccgcggc acgtgaattc
     1801 gtcgcccgca tgctgctcca acaggtcatt gccgatgacg cgtacgaaag atcatggtca
     1861 ggttgtgctt tagttcgtgg cgctttgccg gtcggttggt ggaacgctga tggatttagt
     1921 tgtgacatgg gctggggtac ttcgcaccgg tggcgcgact tggtcgagat ttttgttatc
     1981 gaacttgctg ataatcgagg gcgccagcag tctaagcaag ttcaccggca gactgctggt
     2041 aaaatgatag ttggcggctt cctgtccttg gacaaacgcg gtgagtacgc cgtagtaacg
     2101 ctcgccgatg tagaatacta gggtcgctgt tcggttggtc gcgcgcgaac tgatcacccc
     2161 gccgctgcgg ttgaaggtct caaaacgatt gtctccggag ccggtcttac cgccgaccgt
     2221 gaggaccgtg ccgtcagcga gtttgaacac gccgctaagt ctgcgtgcgg ttccctgttc
     2281 gacgacttcg gccatcgctt tgcgcatggt gtgggcgatc tccggcgcga gcacggcttc
     2341 gccttgattt tgctgttttt ccagaagcgt ttcataaggt gtattgctgg cgaaatggat
     2401 tttcgtcagt gtcgttgccg gccggcgtat gccgtcgttg accagaatgc cgaccagctc
     2461 cgcgagcgct gtcggccggt ccgacgagct gccgatggcg gtggcgtagg tgggcaccaa
     2521 tgatttgaag gggaaaccga gccgttgcca atagggagcc atgcgcaaaa acgcatcttt
     2581 ttcaatacga atgcgcaagc gattcaattg cgcgcgctga ttgcgcgggt tgagcagcca
     2641 ggccgagccc aagtgccgtg ccttcacgct tttgtgatac agcgcttccc aagtcagctc
     2701 gccatttttg cgatactcac tggcgcacca gaggtcgagc gggtgcatgc tgagtagata
     2761 agcgtaatcg accaaagtca atcgtgggtt gtggtaggcg cgaaacagtt tcgtgacatc
     2821 agtttcggcg cttttcgctt catgcttttc cagccaggcg gttaacgctt cctcgtcatt
     2881 gccgattttc caggcgaaaa acaagacggc gagacgacgc tcgatattgc gtttagtgcc
     2941 gagaatccgt tttacgatct cgtcgggtgt ttgtttagcg taagtctgat aggcccggcg
     3001 caaggcgacg cgagattcct cgtcggcaat ttcctgaagc atctgccggc gtgttggatt
     3061 atcgggatta tcgagcacat cgttggcatc atacgcgagt cgggcacggt gataactgac
     3121 caagtcgcgc atcaggcgaa tgaacgccaa gttgttggag ttgcgcaggg catcgcgcag
     3181 ttcgaaaatg cgctcgtttt ccttcttgtc gaagttctcg aaatggtgca gtccgccgcc
     3241 ggtgaaaaaa gcttcatagg gactgccgga atatttccgt tgcatagctt tgtcaaggaa
     3301 cggctgcaag gccatggcgt tatccgtccg tagggtttcc gccgcccatt tggacaacgg
     3361 atcgcgagcg gcgtagccac gttcttgcag ctctttcggt tccagaccgg agagttcctt
     3421 gtgcagctcg gcgactaatt ctaaataatg cgtcagagtg cgcagcttgg cggtgctgcc
     3481 cagttccagc ttaacgcttt tgttgaaatc caaagaggcg gccaaattgt ccgcctgcac
     3541 gcgcaccaga ttgcccgtcg gtgtggcctc cacgagcagg aacgaataag tcatttttga
     3601 tggatcgttg gattctagaa gccgctcgcc gtccagacca taggctttaa tgacttcgcg
     3661 ttgacccaag tgattgaaaa aatccgacac ccgcttctgc aagggtacat caatggtgct
     3721 gtcgacttgc agatgcaggc gattgagatc gtaaaggttg ttcacgccaa gcacctccat
     3781 catgctgacg cggatggcgt tggcggcttt attcttgatc gacgatggct gtggcggcag
     3841 cggcgcggtc ggcagaaatt taatcggcgt ttcttgtaga cgggcggcga attcccaatc
     3901 gattatccct gcgcgagcca tcaggcgggt aaattggttg accttttctt ccagcgaggc
     3961 ccgttcgtcg accaggaaca ctgtcggcgc acggactgaa atcaacaagg tcagggcatg
     4021 tttaaaggcg tgcaccttgg ccggcgtgaa tttgtcagat ttcagatcct taaccgcgtc
     4081 gctcaaacga atgccaaacc aggcgtagag accttcgcca agaccgtgga tttcgccgta
     4141 ggccggcgcg gcagccaggg gtacggtatt caaataatcg acgatgatcc gttcgcgcca
     4201 agcgcgcgtg ttgggacctt cttgataggc ttttaaacta gcgccgatga tctggcgcag
     4261 cttctcaaaa ggatcgtcgg tgcggccatg gggtgaatgg cgaaattttt ccagctgcac
     4321 cgccagggtg ctgccgcctt ggacagaaac cggtagttga agcttcgatc ccagatagta
     4381 aagcgccgct ttgaacgagc ggtcccactc gatcacagga ttttgccagg gtgttgcggg
     4441 ccggtcgaga tcacggttct ctaaaaacaa aagcgttttg accaggagcg atgggatgtc
     4501 gtcgatcttg ccgaagacaa agtcggcctg ggcgtagcga aacagttgca ctccgtcggc
     4561 gcctctgatt tccaagccgg cacccggccc ttcccggtag ggcggcgaaa cgccgcgctc
     4621 caggagggta agcatggtct ccgactgacg ggtttgtttg gttacctggt agccttgaga
     4681 ttgcaggcga gtttgtaatc ccggcagctt ggaatacccg cgtcgctcgt caaaaggcgc
     4741 ggcacggggg aaaatgattt cccggcttgg gccatccttc agcgtataag aaagtcgctt
     4801 gttggtgctg gtgaaaaacc aagattgcag gacggaagtt tggatctcga caaataggca
     4861 ggcggcaagg attgccatgg cggccagcca ccagagccac actttgagtc gcttgaaatt
     4921 gacgaagaac aacaaccgac ctgccagcga cggccccggc aacaactcgg tgggaaggga
     4981 ttttccgctc attgaagtat gcagccgatg atatcacaag ttttgtgaaa atgcttagag
     5041 ggtgctcagg aaccaagact gccacagctc gggagatcct gaaatggagg caggcggaaa
     5101 ggttgtgccg tcggaacaga ttgatgaggt cgcctcaccc gcacggatgc aacgatggca
     5161 cgatttgacg cagagttgca gggagctgta ctaggagtat gcagaccggt agcaatcggc
     5221 gcagcgcacc gggaagctaa cttacgttag gcaccgacgg cgggaaatgc cgcaaagatg
     5281 agcggctcat taaatattg
//
LOCUS       DATLCH010000379        13948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10383419, whole genome shotgun sequence.
ACCESSION   DATLCH010000379 DATLCH010000000
VERSION     DATLCH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10383419"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(552..830)
                     /locus_tag="VMZ02_11355"
     CDS             complement(552..830)
                     /locus_tag="VMZ02_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72578.1"
                     /translation="MNILLRILIGACVGWLTGKAVEIEGRLKVEKEGHVRDVLCGIVG
                     ALAGDLLFFWIVIGKGDGLSSFATAALGAVTVVGAVRLIAERWHAAQS"
     gene            complement(905..1225)
                     /locus_tag="VMZ02_11360"
     CDS             complement(905..1225)
                     /locus_tag="VMZ02_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72579.1"
                     /translation="MFSRNVSYKLKAKSAAEFTRIVEGEVIPLLRRQKGFDDAISFIA
                     PERNEAVTISLWDKKEDAEAYIDKTYPEILKALASVVEGHPKVESFEIGNSTSHQIAA
                     SPVN"
     gene            complement(1403..3112)
                     /locus_tag="VMZ02_11365"
     CDS             complement(1403..3112)
                     /locus_tag="VMZ02_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72580.1"
                     /translation="MSNLGLSSASFLNYLPVPLAFGTSGLRGLVKDITDLEAYINVKG
                     ALRYLMRSGDIHTDSCVVIAGDLRPSTDRIMRACAQAIIDLGCRVENAGRIPTPALIA
                     HAISRRSAGIMVTGSHIPFDRNGIKINKSVGEVLKSDEAGIIDEVEQVRAEEYSRSAS
                     ATAFDVCGMLKCGPELPVVDGAAEAAYVRRYRSSFARGGLSGMRILVYQHSAVGRDLL
                     ARILHELGAEVVSAGRSETFIPIDTENITDEQLERLEALVVSCEINGGPISAVVSTDG
                     DSDRPLVTAVLPAAAVEPGARRVRFLPGDLLGAVVAEYLRADAAAVPISANDAVERWF
                     GERNVVLAKTRIGSPFVVAALEDLRATGKHKRIVGWEANGGFLTGTDIELNGATLTAL
                     PTRDALLPILANLFAAVEQGVSLATLWERLPARFGRAGLLDNFPVAASQAILAQLIPP
                     GTVVEVEFDGGSVVDCSRVDGARIRLAGSAAKDWQHCQATLTRYFTPALGFDAIARVN
                     VLGGVRVYFRNGDIAHIRPSGNAPQLRIYAVSASQKRADRIVELALAEPDGILRRLER
                     AFS"
     gene            complement(3200..3544)
                     /locus_tag="VMZ02_11370"
     CDS             complement(3200..3544)
                     /locus_tag="VMZ02_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HUR72581.1"
                     /translation="MNTPRENVDEPFDERANEDGYFAAKEHALVEDMRLELQKAEAAR
                     RQAEIATCPKCSGKLQEYPLLGVVVERCVNCQGLWLNNGELDEILRQRARGPLALFLD
                     RCFAKTAPQKTN"
     gene            complement(3662..4165)
                     /gene="nuoB"
                     /locus_tag="VMZ02_11375"
     CDS             complement(3662..4165)
                     /gene="nuoB"
                     /locus_tag="VMZ02_11375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011393.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HUR72582.1"
                     /translation="MSLINQLPETVFTTKVDELLNWGRASSQWYMLFGLACCAIELMQ
                     TGGPRADVDRFGAVPRATPRQSDLMIVAGTLTYKMAKRTKLLYDQMPDPKYVISMGSC
                     SNCGGLFQLAYSVCKGVDKIIPVDVYVPGCPPRPEALTEGLLRIQDKIMREKWLVKDT
                     PAASVPA"
     gene            complement(4200..4427)
                     /locus_tag="VMZ02_11380"
     CDS             complement(4200..4427)
                     /locus_tag="VMZ02_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72583.1"
                     /translation="MKSEISYKNYFIRCRSFQPTRNSDWIPRYVLQRAHALNGADASP
                     LSRDRLDKAFSSASEADDFAVQDAKNWIDNN"
     gene            complement(4846..5358)
                     /locus_tag="VMZ02_11385"
     CDS             complement(4846..5358)
                     /locus_tag="VMZ02_11385"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HUR72584.1"
                     /translation="MTLSIGEKVSLPSHGPCVIGAVVNKLVGGTSTAFYRLAPLEDNG
                     GELFIPVDKITGLGIRQLLQKSEIPKLLGRMSQPGKTVPPPSTARNWKQRALDNGKLL
                     SSGSASDLVQIVESLSDLNETKTLAPHERQTLERAKKLLICEITEVLGQPRSAVESQI
                     EKALQSRKAG"
     gene            complement(5536..5733)
                     /locus_tag="VMZ02_11390"
     CDS             complement(5536..5733)
                     /locus_tag="VMZ02_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72585.1"
                     /translation="MGTETFQKRQKENARKEKHKLKLARRLERKNEKVPGENSLDVDI
                     LELERALEQSEQSTDQRGPTS"
     gene            complement(5745..6713)
                     /locus_tag="VMZ02_11395"
     CDS             complement(5745..6713)
                     /locus_tag="VMZ02_11395"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72586.1"
                     /translation="MNNQHPELFRRNQLNPILSAGDWPYPVNSVFNAGATRLADGTTL
                     LLCRVEDRRGHSHFTVARSDNGVDHWRIESKPTLLANPENFPEEIWGIEDPRITYLPE
                     LGKYAIVYTAFSRDGPGVSLALTQDFHSYERYGVVMSPEDKDAALLPRRIGGQWALIH
                     RPVSAPRAHMWISFSPDLHHWGNHKLMLEARRGSWWDANKIGLSPPPIETAQGWLVIY
                     HGVRQTAAGSIYRLGLALFDLQAPESCINRGDEWVFGPQEVYEQRGDVDNVVFPCGYT
                     IAPDGDTIQLYYGAADTSIGLATGSIHAMLEWLGRHGDKPTTTLSD"
     gene            complement(6713..8998)
                     /locus_tag="VMZ02_11400"
     CDS             complement(6713..8998)
                     /locus_tag="VMZ02_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HUR72587.1"
                     /translation="MTQGLQNTAISRIAFVGNYLPRQCGIATFTTDLCEAIAAEHSDT
                     TCIAVPVNDIEGGYAYPSRVRFELTEKDLESYRRGADFLNINSVDLVCLQFEYGIFGG
                     RAGSHILTLLRDLRMPIVTTLHTILREPDAQQRRVLTEVAGLSDRLVVMTKRSAEFLQ
                     EIYQIAPEKIDLIPHGIPDLPFVDPSFHKDLFGVEGKIVLLSFGLLSANKGMETVISA
                     LPAILAKHANVVYFIVGATHPHVLRHDGETYRLSLQWLAQEKGVEGQVIFYNRFVSLE
                     ELVEFIGAADSYITPYLNPAQAVSGTLAYTVGAGKAVISTPYWHAEEMLAEERGVLVP
                     FRDPTALAEQVIDLLDNEAKRHSMRKRAYLYGREMVWPQVARRYMETFQRARAERRYF
                     TPPDFVVKPRDKRPAELPPLKLDHLRRMTDDTGMFQHAIFTVPNYQEGYTIDDNARAL
                     TVSALLEELGNEETADLMSRYFAFVWYAFNAKSGRFRNFMSYQRDWLEENGSDDSHGR
                     ALWALGTVLGRSNTPALQNMAGWVFERSLPAVLETTSPRAWAFALIGINEYLQRFAGD
                     RTITQAREVLAGRLLELYRQNRSERWRWYESSLSYCNAVLPHALLMCGRGIPNADMTD
                     AGLESLAWLADLQRAEVTGGHFVPIGSNGFYTRDGARARFDQQPVEAQAMVSACLEAQ
                     RITGDKNWSKEARRAFDWFLGRNDLNLPIYDPTTGGCRDGLHPDRTNENQGAESSLAY
                     LQALLELRLAETALVSATTGV"
     gene            complement(9380..9727)
                     /locus_tag="VMZ02_11405"
     CDS             complement(9380..9727)
                     /locus_tag="VMZ02_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72588.1"
                     /translation="MDWDKIKLGGWSAAGGAVLAMIVGFNWGGWVTGGTADVLAKEIA
                     ENAVAKRFTPSCVARFNQDAKKEQKLKEIKAKDAWDGQKFVEEQGWATLPGEERPDSK
                     VAEGCAKSLMAKS"
     gene            10203..11126
                     /locus_tag="VMZ02_11410"
     CDS             10203..11126
                     /locus_tag="VMZ02_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HUR72589.1"
                     /translation="MAPIISISNLTKTYASGHQALKRVDLEIYNGEIFALLGPNGAGK
                     TTLINVVCGIVTPTQGTIIAAGHDILREYRAARAKIGLVPQELSTDAFESVWVTVSFS
                     RGLFGHPPKPAYIEEVLRELSLWDKRDNKIMTLSGGMKRRVMIAKALAHEPQILFLDE
                     PTAGVDVELRRDMWALVRRLRDNGVTIILTTHYIEEAEEMADRIGVINKGELILVEEK
                     STLMKKLGKKQLTLNLLAPLAKLPPELDAWQMALNAGGMELEYTFDAAEERADIPALL
                     QRLSDLGIRFKDLNTRQSSLEDIFVSLVSDR"
     gene            11440..12216
                     /locus_tag="VMZ02_11415"
     CDS             11440..12216
                     /locus_tag="VMZ02_11415"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HUR72590.1"
                     /translation="MSNLAFNRHSVWAIYKFEMARALRTLFQSVVSPVITTSLYFVVF
                     GAAIGARMAQVDGVAYGAFIVPGLIMLSLFTQSISNASFGIYFPKFTGTIYELLSAPV
                     SFLEIVVAYVGAAASKSIALGLIILATAAMFVPVRIQHPLWMIAFLVLTAATFSLFGF
                     IVGIWAKTFEQLQFIPMLIITPLTFLGGAFYSIDMLPPAWRTVTLFNPVVYLISGFRW
                     SFYESSDVSIEISLAMTLAFFALCLAVVGWMFKTGYRLKS"
     gene            12363..13652
                     /locus_tag="VMZ02_11420"
     CDS             12363..13652
                     /locus_tag="VMZ02_11420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HUR72591.1"
                     /translation="MSVSPLVEQPVRKKAIFYGWVIVVVGFLAHIASAFSISSTLAVF
                     LKPLSADLGISRGMFSLIRSGEILIGAAAAPVVGTLMDRYGGRWLMAAGGLISGAGFI
                     CLGQAQGFIQFMLVRWLLISPGDSLMGSMVVNVSISRWFVRMRGRALAIAGMGHGLAK
                     VCMPVAAASLMLYVGWRGAWVVFGVFALVLVVAPALLFMRRSPEEMGLLPDGGPAQGK
                     AAIVTPKKTRYAKRTGADDVEWSRAEALRTPVFWLIASTFGVAQIGVTGLNLHVFSYV
                     TDQGHPILVAASVMSIIAVMQLSTPIVWGTLADRMDNGILNMAKCAFQGAGLLVALLL
                     PGMAPLYIGFFIYGIGMGGTAVLAETIWADFFGRVSLGRIRGMGSLVTSIFSACGPPF
                     FGLLFDYTQSYYLSFSIFIAMLFTSAVLSLCLRPPKK"
     gene            13684..>13948
                     /locus_tag="VMZ02_11425"
     CDS             13684..>13948
                     /locus_tag="VMZ02_11425"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72592.1"
                     /translation="MKIDSNDFRVREDKNVNLKKWPTEVKPYYDSKKHFKEILRERVG
                     KLSDLQSILYAADSYAVLLIFQAMDAAGKDSAIKHVMSGVNPQG"
BASE COUNT         2978 a         4052 c         3802 g         3116 t
ORIGIN      
        1 cacgtgacac acggatagga atgaaaatag ccggcgagaa tcatcgctga aaaaccaatc
       61 ttccaccgcc cgggtgcgtt ggggaaaacg cgcgtgggca aggaaccgtt tttgataaca
      121 gagaaaagca tcttcgagga cggcgtacat caaagcggtt tccgggcaga cggtcgcaag
      181 tttaacccgg cagttgaaga atgtttcgac aagcaatcca tcagttctag gccgctcata
      241 taaagcatcg cgcgcggcac agacaggctg tcgcgatttt agccgaatag caggtacacg
      301 ggtcggtcgt ggcatgtcat tgccctcctc aaaagatggg ggacaaagac tatccgatgc
      361 cgggagtcaa atctgttcaa tattggacaa gatgttgcgc cttgaaggca aaacaacatg
      421 gtcaccaaag cgagctaacg cgaacgggcg acgaatcgga gagctcatga ctcatcgcgg
      481 ccgcggcgca aatcacgata ctcgcgatgg agaacgcgac gataacgagt ctgggaaaac
      541 gcgttttcta tctacgattg agccgcgtgc cagcgctcag ctatcaagcg gacggctccg
      601 acaactgtga cggcgccgag tgcggcggtt gcaaaactac tgagcccatc gcccttgccg
      661 ataacaatcc aaaagaacaa taaatcgccg gcaagcgcac cgacaattcc gcatagcacg
      721 tcgcgaacat gtccttcctt ttcgacctta aggcggccct cgatctcaac cgcctttccc
      781 gtcaaccaac cgacgcatgc gccaatgagt atgcgaagta gaatgttcat cacgcttccc
      841 tccggcagcg cgttacggcg gtccggcaac gagagtcccc gtatcgccga accgccatcg
      901 ctatctagtt gaccgggctg gcggcgatct ggtgcgaggt cgagttgccg atctcgaagg
      961 actccacctt gggatgacct tcaacgacac tcgccagcgc ctttaaaatc tccggatacg
     1021 tcttgtcgat atacgcctcg gcgtcctctt ttttatccca cagactaatc gtgacagctt
     1081 cattgcgttc cggagcgatg aagctaatcg cgtcgtcgaa acctttttgt cgtcgcagca
     1141 agggaatgac ttctccctcg acgatgcgcg tgaactccgc tgcgctcttg gccttgagct
     1201 tgtaactgac gtttctactg aacattgcaa cctcctgggt tggaccggtt agaaagaaaa
     1261 attgaagctg tcttgcctga agaatttcag gtaagcgccg atgactagtc gaactaaact
     1321 agaacctatt actataactc agacgtagaa acttactgtc taatattaga cggatctcag
     1381 gattgtatcg ccgctcacac gtttacgaga atgcacgctc aagccggcgc aagataccgt
     1441 caggctccgc gagcgcgagc tcgacgatgc ggtccgcgcg tttttgcgaa gcgctgaccg
     1501 cgtagatgcg cagctgcggc gcatttcccg acggccggat gtgcgcaata tcgccgttgc
     1561 ggaaataaac ccgtacgccg ccaagcacgt tcactctggc tatggcatcg aagccaagcg
     1621 ccggcgtgaa atagcgcgtc aacgttgctt ggcaatgctg ccaatcttta gctgccgatc
     1681 cggcaaggcg tatgcgagcg ccatcgacac gactgcaatc gacaacactg ccgccatcga
     1741 actcgacttc gacgaccgtg ccgggtggga tcagttgcgc gagaatcgcc tggctcgctg
     1801 ccacagggaa gttatcgagc aagccggccc ggccaaaacg cgccggcaaa cgctcccaca
     1861 gcgtggccaa gctgacaccc tgctccacgg cggcaaagag attggccagg atcggcagca
     1921 gcgcgtcgcg ggtgggcaag gccgttaaag ttgcgccgtt caattcaata tcagtgccgg
     1981 ttagaaatcc gccgttggct tcccagccaa cgatacgttt gtgtttgccc gtggcgcgca
     2041 aatcctcgag cgctgcgacg acgaacggcg aaccgattct ggtcttggcc aggacaacgt
     2101 tacgctcgcc gaaccagcgt tcgacggcat cgttggcact gatcggcacc gccgcggcat
     2161 cggcgcgcag atactcggca accacagcgc caagcagatc gcccggcaag aaacgcacgc
     2221 gccgcgcgcc gggctcgact gcggcagccg gtagtacggc ggtgaccagc ggtcgatccg
     2281 aatctccgtc cgtcgataca acggcggaaa tcggcccgcc gttgatttcg caagaaacaa
     2341 ccagcgcttc caggcgctct aactgttcat cggtgatatt ctcagtatcg atgggaataa
     2401 aggtttcact gcgacccgcg ctcaccacct cggcacccaa ctcgtggaga atccgcgcca
     2461 gcaaatcgcg gccgacagcg gaatgctgat aaaccaatat gcgcatgccg gatagtccgc
     2521 cacgagcgaa gctactgcgg tagcgtcgca cgtaggctgc ttcggcggcg ccatcgacca
     2581 cgggcaattc cggcccacac ttaagcattc cgcaaacatc gaatgccgtg gcgctcgcgc
     2641 ttcggctgta ctcttcggcg cgcacctgtt cgacttcatc gatgatgccg gcttcgtcgg
     2701 atttcagcac ttcgccgaca cttttattaa ttttaatgcc gttacgatcg aacgggatat
     2761 gactgccggt gaccattatg ccggcgctgc gtcgtgagat cgcgtgcgcg atcaatgccg
     2821 gcgtaggaat cctcccggcg ttttccaccc gacaccccaa gtcaataatc gcctgagcac
     2881 aggcgcgcat gattcggtcg gtgctcggcc gcaggtcgcc ggcaatgacc acacagctgt
     2941 cagtgtgaat atcgccgctg cgcattaaat agcgcagcgc gccctttaca ttgatgtaag
     3001 cttccaggtc ggtgatgtct ttcaccaggc cgcgtaaacc actggtgcca aacgcgagcg
     3061 gaaccggcag gtaattgaga aacgacgccg aggacagacc aagattgctc ataggcttca
     3121 ttttaccgag gaatcaattc tcaagcgagc aaaccacgac gggaaatgcg tcgcactgat
     3181 caacgagctg tcagggtctt tagttggttt tttgtggtgc ggtcttggca aaacagcgat
     3241 cgagaaaaag cgccaatgga ccgcgtgccc gctgccgcag tatttcgtca agctcaccgt
     3301 tattgagcca aagaccttgg caattcacac atcgctcaac gacgactccc agaagcgggt
     3361 actcttgcaa tttccccgaa cacttggggc aagtcgcgat ctcggcttga cgccgcgctg
     3421 cctcggcttt ttgcaactcg agccgcatgt cttcgacgag cgcatgttcc ttagcggcga
     3481 aatagccgtc ttcgttggcg cgctcatcga aaggctcgtc gacattctcc cttggcgtgt
     3541 tcatcaatcg aatccttgtc ggccgacttt tcggattcct tcgctgtggg attaccgagt
     3601 tagccgatgc ggttgcggca gtatggatga tttgtatgcc tgccccgacc cagtttgcat
     3661 tttaagccgg aaccgatgcc gccggcgtgt ccttgaccag ccacttctcg cgcatgatct
     3721 tgtcctgaat tcgcagaagc ccctcggtca aggcctcggg acggggcggg caaccaggca
     3781 catagacatc gacggggatg atcttgtcga cgcctttgca gaccgaatag gcgagctgaa
     3841 aaagtccacc gcaattggaa cagctgccca tcgagatcac atacttgggg tcgggcatct
     3901 gatcataaag aagcttggtc cgtttggcca ttttgtaggt caacgtgccc gcgacgatca
     3961 ttagatccga ctgacgcggt gtcgcccgcg gtacggcgcc gaagcgatcg acgtcggcgc
     4021 gcggtccgcc ggtctgcatc agctcgatgg cgcagcacgc caggccaaag agcatgtacc
     4081 attgggagga agcgcgtccc cagttgagca gctcatccac cttggtggta aacaccgtct
     4141 cgggaagttg gttgattaag gacataaaga tctccgttta cctgaccgct agtcggccct
     4201 taattattgt cgatccagtt cttggcatct tgcacggcga aatcgtccgc ctcagacgcg
     4261 ctgctgaagg ctttatcgag ccggtcgcga ctcagcggcg aagcgtcggc gccgttgaga
     4321 gcatgggcac gctgcaacac ataacgcggt atccaatcgc tgttgcgcgt cggctgaaac
     4381 gatcggcagc ggatgaaata gttcttgtaa gaaatttcag atttcatttg ctctagcctc
     4441 cgctaaaggt cgcaggagca cattgcaaat agaggcttcg aatggggcaa cggcgatcgc
     4501 ctacgacagt aaagagcctc attaagccta ggcaatctaa ggcgtgatag caaggcgaca
     4561 ctatctacgt gttacgatcc atccgcctcg aaaatgggag aatgctacca gggtttcggc
     4621 tgtgtgatca cctgctatag tcgcgcaaat gacgacctat gtttagtgac gtaagcctaa
     4681 gggatctact agtggagact taccgtcccg gccttcactg cacatccgac agctttcaac
     4741 taccgaaatc ccaccggctt cccagcgcct gcaaccaacc cggcgatgtt ggagcaaatg
     4801 ttcctggcga accgtatcgc tgggagtcta gctaggacaa aatgctcagc cggctttgcg
     4861 gctttgtaaa gctttttcga tctggctttc caccgcgctc ctcggttgac ccaagacttc
     4921 ggtgatctcg caaatcagca gcttcttcgc ccgttccaaa gtctggcgtt catggggtgc
     4981 cagcgtcttg gtctcattca agtcgctcag cgattcgacg atctgaacca gatcggacgc
     5041 cgatcccgaa gacaacaatt tgccgttatc gagggcgcgc tgtttccagt tgcgcgctgt
     5101 cgacggtggc ggcacggttt ttcccggttg gctcatgcgg cccagtaatt tgggaatctc
     5161 cgatttttgc agcaactggc gaatgcccaa tccagtgatc ttatcgaccg gaataaacaa
     5221 ttcaccgccg ttatcttcca ggggcgccaa gcggtaaaat gccgtagacg taccaccgac
     5281 caatttgttc accactgcgc cgatcacaca aggtccgtga ctcggcagac tgactttttc
     5341 gccaatactc aatgtcatgc gattctcctc tgttagctgg acgccgcgcg aactaatccg
     5401 tgaaccgttc attgttgtta agttgcaccc gtgaaatcga cccgcctgtc gccgttctcc
     5461 gacgagtttg cgatttgcgc accctctctg acggtcaagg cgagcgagat ttaagttttt
     5521 aagcagctcg gagtttcagc tggttggccc gcgctgatcc gtggactgct cggattgctc
     5581 gagcgcgcgc tcaagctcca ggatatccac atccaaactg ttttcgcccg gcactttttc
     5641 gttcttacgc tccagccgcc gagctaattt cagcttgtgt ttttccttcc gggcattttc
     5701 tttctggcgt ttttgaaatg tctcagtacc cataaccgct cctttcagtc gctaagagtt
     5761 gtcgtcggtt tgtcaccgtg acgtccgagc cactcgagca tggcgtgaat gcttcccgtg
     5821 gccaagccaa tgctggtgtc cgccgcgccg tagtagagtt gtatggtgtc gccgtccggc
     5881 gcaatcgtat agccgcaagg gaacaccaca ttgtcgacat cgccacgttg ttcgtaaact
     5941 tcctgggggc cgaaaaccca ctcatcaccc cggttaatac aagattccgg cgcctgcaag
     6001 tcaaacaagg ccagcccaag tcgataaatc gaccccgcgg cagtttgccg gacgccatgg
     6061 taaatcacca gccacccctg cgcggtttcg ataggcgggg gggaaagacc tattttgttg
     6121 gcgtcccacc acgatcctct gcgcgcttcc agcatcagct tgtgattacc ccagtgatgc
     6181 aagtctggtg aaaacgagat ccacatgtgc gcccgcggcg cgctgaccgg ccgatgaatt
     6241 aacgcccact ggccgccgat gcgacgcggt agcaaggcgg catctttgtc ttccggtgac
     6301 atgaccacgc cgtagcgctc gtagctgtga aaatcttggg tgagcgccaa agatacgccc
     6361 ggaccatcgc gtgagaacgc ggtatagacg atggcgtact tgccaagttc cgggaggtaa
     6421 gtaatgcgcg ggtcttcgat accccagatc tcctcgggaa aattttccgg gttagccaaa
     6481 agtgtgggct tggattcgat gcgccagtgg tcgacaccgt tgtcggaacg ggcgacggtg
     6541 aaatgcgagt gaccgcggcg atcttcgacg cggcacaaca gcagcgtggt gccgtcagcc
     6601 aagcgcgtcg cgccagcgtt gaaaacgcta ttgacgggat agggccaatc gccggcgctc
     6661 aggatcggat tcaattggtt gcggcgaaaa agttcgggat gctgattgtt catcaaactc
     6721 ctgtggttgc ggataccaaa gcggtttcag ccaagcgcag ttccaacaac gcctggagat
     6781 aagcgagcga agattcagcg ccttgattct cattggtgcg gtcgggatgc aagccatcgc
     6841 ggcagccgcc ggtggtcgga tcatagatcg gcagattcaa atcgttgcgg cccaagaacc
     6901 aatcgaaagc ccggcgcgcc tccttggacc agttcttgtc accagtaatc cgctgcgcct
     6961 caagacaagc cgagaccatc gcctgggcct ccaccggctg ctgatcgaaa cgcgcgcgcg
     7021 cgccgtcgcg agtataaaat ccgttggagc cgatgggcac gaaatgaccg ccggtcactt
     7081 cggcgcgctg caagtccgcc aaccaggcga gcgactccag tccggcatca gtcatgtcgg
     7141 cgttgggtat accgcgcccg cacatgagca aagcgtgcgg cagcacagcg ttacaatagc
     7201 tcaacgatga ttcgtaccaa cgccagcgct ctgagcggtt ttgccggtag agctccagca
     7261 atcgaccggc gagaacctcg cgtgcttgag taattgttcg gtcgccggca aatcgttgca
     7321 agtattcatt gatgccaatc agggcgaagg cccacgcccg cggactcgtg gtctcgagta
     7381 ccgccggcaa cgatctctca aagacccagc ctgccatatt ttgcagcgcc ggcgtattgg
     7441 aacgacccaa gaccgtacca agcgcccata acgcgcggcc atgactatcg tcggaaccgt
     7501 tctcctccag ccaatcgcgc tgatagctca tgaagttgcg gaagcggcca ctcttggcat
     7561 tgaaggcata ccagacaaac gcgaagtagc gcgacatgag atcggcagtc tcttcattac
     7621 cgagttcttc caaaagcgcg ctcaccgtca gagcgcgcgc gttatcatcg atggtgtagc
     7681 cctcttgata attgggcacg gtgaaaatcg cgtgttgaaa catgccggta tcgtcggtca
     7741 tgcggcgcaa gtgatcgagc tttaacggcg gcaactcggc gggtcgcttg tcccgcggct
     7801 taacgacaaa atccggcggc gtgaaatatc gccgttcggc gcgcgcgcgc tgaaaagtct
     7861 ccatgtagcg ccgcgccacc tgcggccaaa ccatttcgcg gccgtaaaga taggcgcgct
     7921 tgcgcatgct atggcgcttg gcctcgttat cgagcaaatc gatgacttgc tcggccagag
     7981 ctgtcggatc gcgaaatggc acgaggacgc cacgttcttc agcaagcatt tcttcggcat
     8041 gccaataggg tgtagaaatc actgccttac ctgcgccgac cgtgtaagcg agcgtcccag
     8101 agaccgcctg tgcggggttt agatatgggg taatgtaact gtccgcggca ccgataaact
     8161 cgacgagttc ttccaagcta acgaaacggt tgtaaaaaat aacctggcct tcgacgcctt
     8221 tttcctgcgc caaccattgc agcgataagc ggtatgtttc gccgtcgtgg cgcaacacgt
     8281 ggggatgggt cgcgccgacg atgaagtaga ctacattggc atgcttagcg agaattgccg
     8341 gcaaagccga gatgacggtt tccatcccct tgttcgccga cagcaagccg aagctaagca
     8401 gaactatctt gccttcgacg ccgaacagat ccttgtgaaa actcggatcg acaaatggca
     8461 gatcgggaat gccatgggga ataagatcga tcttctccgg cgcaatttga taaatctctt
     8521 ggagaaactc cgcgctacgc ttggtcatca cgaccaaacg gtccgacagg cctgcgactt
     8581 cggtcagtac gcgccgctgc tgcgcatcag gttcgcgcag gatcgtgtgc agcgtggtaa
     8641 cgatgggcat gcgcagatcg cgcagcaggg tgagaatatg gctgccggcc cggccgccaa
     8701 aaatgccgta ttcgaattgc aaacaaacca ggtcgacgct gttaatgttc aaaaaatcgg
     8761 cgccccggcg gtacgattct agatcttttt ccgttagctc gaagcgcacg cgcgacggat
     8821 aggcgtaacc accttcgata tcgttaaccg gcacggcaat acaggtcgta tcgctatgtt
     8881 cggcggcgat cgcttcacac aggtcggtgg taaaagtcgc aatgccgcat tgccgcggca
     8941 agtagttgcc gacaaaagcg atgcggctga tcgcggtatt ttgcagcccc tgggtcactg
     9001 gaaaagcact cctagctggc gattatgcct tgcggttatc gatcaggctc cggcctacaa
     9061 agacaaagag gcggttacct gggtaccgcc tctttgtgac tcgggaaaaa taaccgagtt
     9121 tgttctatgg gaacgtttag gtcagtagtt gctatggatc tgattgtagc agacgaggct
     9181 gataaagata gcccctactt agttttgggg accggcgccg tttgctttgc cagcgcattt
     9241 tttgccgctg tattctgttg tatttgcttc tgcttctggt gcttgttttt atcttttttg
     9301 ctcttgtcgc ccatttaaac tcctatcgcc ggaactgatc cagcggatgc tttgatgttg
     9361 aggcgcagac ccgtgaaact tacgacttcg ccatgagact tttggcacaa ccctcggcga
     9421 ccttactatc ggggcgttct tcccctggca acgtcgccca gccctgctct tcaacgaatt
     9481 tttgcccgtc ccaagcgtcc ttggccttga tctctttaag cttttgctcc ttcttcgcgt
     9541 cctggttgaa gcgcgcgacg cagctcggtg tgaagcgttt ggccacggcg ttttcggcga
     9601 tctctttggc cagcacatcg gccgtgccgc cggtcaccca accgccccaa ttaaacccga
     9661 cgatcatggc cagcaccgcg ccaccggcag cgctccagcc gcctaattta atcttgtccc
     9721 aatccatcga atacctccct catattaatg cacagatgga atccatagac ttaacgacgc
     9781 gcagctctgg caaatccagt aatcggagta gcgctgcgac accgagcact cctggcaaac
     9841 ccggcaatag cgatgaccgc aggactcgca tttaatgccg ctcgccggat cgtccacaca
     9901 cttcgcctcg atcatggttg ttttgaattg gccgcgccag aaactcacac caggaatggt
     9961 ctggcgcttc ctcaccgcgc ggcgtcgcgc agcatctcga acctttgcat tctcggcgat
    10021 attaaacgct cgcgacagcg attactgtct aatagtagac aatcgccaat atttattttt
    10081 ttccttgaat agccgtgcgg cggcaaccct ccgggttaaa ggtgctgggc actcccttgc
    10141 gctactctac aaggttaagt caatccttac agaggttctg cgattacgtt aagatcgcag
    10201 cgatggcgcc aatcatttca atctccaatt tgactaaaac ctacgcttcc ggccaccagg
    10261 cgctcaagcg cgtcgatctg gagatctata acggtgagat tttcgcgctg ttaggtccga
    10321 acggcgccgg caagaccacg ctgatcaacg tcgtgtgcgg cattgtcacg ccgactcaag
    10381 gcaccatcat cgccgccggt catgatattc tgcgcgaata tcgcgccgcg cgcgccaaga
    10441 tcggcctagt gccacaagag ctcagcaccg acgcgttcga gtcggtgtgg gtgacggtga
    10501 gcttcagccg tgggctgttc gggcatccgc ccaagccggc ctacatagag gaagtgctgc
    10561 gcgagctgtc tttatgggat aagcgtgaca ataagatcat gacgctctcc ggcggcatga
    10621 agcggcgcgt tatgatcgcc aaggcactgg cccacgaacc gcagatcctg tttctcgacg
    10681 agcccaccgc cggcgtcgat gtcgaactgc gccgcgacat gtgggccctg gtgcgccgcc
    10741 tgagagacaa cggcgtgacg atcatcttga ccacccacta catcgaggaa gccgaggaaa
    10801 tggccgaccg cattggcgtc atcaacaaag gcgagctaat cctggtggaa gaaaaatcga
    10861 ccttgatgaa gaagctcggc aagaaacagc taacgctcaa tctgctcgcc cccttggcaa
    10921 aactcccgcc ggaactcgat gcttggcaaa tggcgctcaa tgccggcggc atggaactgg
    10981 aatatacctt tgatgccgcc gaggaacgcg ccgacatccc cgccctactc caacgtctca
    11041 gcgatctcgg cattcgtttc aaggacttga acacgcgcca aagttctctc gaagatatct
    11101 tcgtcagtct ggtcagcgat cgttaagaga gacaagtgtt ccagtcgttc caatcctcaa
    11161 acagttcgag ccgttcaagc cctattcaga gcatagtttt ccgtctgtgt aacctttatg
    11221 ctttggttcg caggcacgag gcgacgaatt gcaccagctt ccgtcatacc ggcgaaagcc
    11281 ggtatccatc cccttccctc cctcggctgc cggcctgcgc cggcataacg actaacagaa
    11341 aaacgctaag gacgggtaca ttgtaatttc atcctgttga cgagcgtaag ctaatgaacg
    11401 ttgtggttat atttccaatt cgtccgagtt aaagacatca tgagcaatct cgcattcaac
    11461 cggcacagcg tctgggcgat ttacaaattc gagatggcgc gggcgttacg cacgctgttt
    11521 cagagcgtgg tctcgccggt catcacaaca tcgctttact tcgttgtctt tggcgccgcc
    11581 atcggcgcgc gcatggctca agtcgacggc gtcgcctacg gggcgtttat tgtgccgggg
    11641 ctaattatgc tttcgctgtt cacccagagc atttccaatg cgtcgttcgg catttacttt
    11701 cctaaattca ccggcaccat ctacgaattg ttgtcggcgc cagtgtcttt tttggaaatc
    11761 gtcgtcgcct acgtcggtgc cgcggcatca aagtccattg ctctgggact gatcattctc
    11821 gccactgccg cgatgttcgt tccagtgcgc atccagcacc cgttgtggat gatcgccttt
    11881 cttgtgctga ccgcggcgac cttcagtctg ttcggcttca tcgtcggtat ttgggcgaag
    11941 acctttgagc agttgcagtt cattccaatg ttgattatta caccgctgac gtttctgggc
    12001 ggcgctttct attccatcga tatgctgccg ccagcctggc gcacggttac ccttttcaat
    12061 ccggtcgtct atttaatcag cggattccgc tggagcttct acgagtcatc cgatgtgagc
    12121 atcgagatta gcctggcgat gaccttggca tttttcgcgc tttgcctcgc agtcgtcggc
    12181 tggatgttca agaccggcta ccgactgaag agctaaaata atttttcacg ctgggcgaca
    12241 aagaatactt agcttgtccg cgtccgcgct gcttaaccag cacgactccg atttgaccgg
    12301 ttacctcagt caaaaacccg gcctgcttgt agtttcgccg ccatccttgc tacaagcgaa
    12361 cgatgagcgt ttcacctttg gtcgagcaac cggttagaaa aaaggctatt ttttacggct
    12421 gggttatcgt cgttgtcggc tttctcgccc acatcgcttc ggcattttcg atctccagca
    12481 ccctggcggt atttctcaaa ccgttgtccg ccgacctcgg catttcccgc ggtatgttct
    12541 cgctcatccg ttccggcgag attctcatcg gcgcggcggc agcgcccgtg gtcggcaccc
    12601 tgatggaccg ctacggcgga cgctggctca tggcggccgg cggtttgatt tccggcgccg
    12661 gttttatctg cctcggtcaa gcgcagggtt tcatccagtt catgctcgtg cgttggctgc
    12721 tgatcagtcc gggcgattcg ctcatgggct cgatggtggt caacgtgtcg atttcgcgct
    12781 ggttcgtgcg tatgcgcggg cgcgccctgg cgatcgccgg catgggccat ggcttggcca
    12841 aggtctgcat gccggtggcg gcggcgagct tgatgcttta cgtcggctgg cgcggcgctt
    12901 gggtggtctt cggcgttttc gcccttgtgc tggtggtcgc tccggccttg ctgtttatgc
    12961 gccgttcccc ggaagaaatg ggattgctcc ccgatggcgg accggcgcag gggaaagcgg
    13021 cgatcgtaac gccgaaaaaa actcgctacg ccaaacgcac cggcgccgac gatgtcgagt
    13081 ggagccgggc cgaagcgctg cgcacaccgg tcttctggct catcgccagc accttcggcg
    13141 tcgctcagat cggcgtcacc ggactcaatc tccatgtgtt ttcttatgtc accgatcaag
    13201 gccatccgat attggttgcc gcctcggtga tgagcatcat cgccgtcatg cagctcagca
    13261 cgccgatcgt ctggggcacg ctcgccgacc gcatggataa cggcattctc aatatggcca
    13321 aatgcgcttt ccaaggcgcc ggcctgttag tggccctgtt actgcccgga atggcgccgc
    13381 tctatatcgg cttttttatc tatggcatag gcatgggtgg cacggcggtc ctggcggaaa
    13441 ccatttgggc ggactttttc ggccgcgtct cgctcggaag aattcgcggc atggggtcac
    13501 tggtgaccag catcttttcc gcctgcggtc cgccgttttt cggcctgctc ttcgactaca
    13561 cacagagcta ctacctgtcc ttcagcattt ttatcgctat gctgttcact tccgccgtgc
    13621 ttagtttgtg ccttaggcca ccgaagaagt gacgccagct cgacccgaca aacaggagtc
    13681 gtcatgaaaa tagattccaa tgactttcgc gtacgcgagg acaaaaacgt caatctcaag
    13741 aagtggccca cggaggtgaa accctattac gattcgaaaa aacatttcaa agaaattttg
    13801 cgcgagcgcg tcggaaagtt gagcgatctg caatctatcc tctatgctgc ggacagctac
    13861 gcggtgttat tgatctttca ggcgatggat gcggccggca aagacagcgc gattaagcat
    13921 gtgatgtccg gcgtcaaccc ccagggct
//
LOCUS       DATLCH010000380         4454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8156953, whole genome shotgun sequence.
ACCESSION   DATLCH010000380 DATLCH010000000
VERSION     DATLCH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8156953"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VMZ02_11430"
     CDS             <1..583
                     /locus_tag="VMZ02_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HUR72593.1"
                     /translation="VLGTTYLETSVNGDDPKPRGNHYALAAPHGVYRCQGDDRWCVIS
                     VRDDVEWRNFCRVIGRVELMSDSRFTSLQSRIDNRAALDGIVSAWTMARTAEEVMTEC
                     QSAGVPASVVQTGADLLKDPQLRHRNYFSAFPESLIGPFEIPRSGFVFNGMAEDPLEL
                     PARFGSDNDQILGELLGYDQATIDQWREEEVLT"
     gene            682..1650
                     /locus_tag="VMZ02_11435"
     CDS             682..1650
                     /locus_tag="VMZ02_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72594.1"
                     /translation="MALPLSPMLAILFLVFLTPDTCAQERVKLRISSAAKTLGYGPLW
                     IASRMGFFDRQGLDVDLVVIRASDIGIQALAGGSLEIAGSSADAPIAAVEKGLDLVIV
                     GGIINGLTQSIMAAKKFKTYNDLRGATFGAISLTSGVTFALRQVLKLKGLEYPRDYKL
                     LVIGGTPQTYAALVAGSIDAAALSLPVNYAAEEQGFNEIGRFVDIMPNYQLAALSVQR
                     SWGEKHRPVLVRVMRAMAQTMRWIHQNKEPAANYLAKEMNLKPDHARRGWEFYTTTKM
                     WHPDGDLNLEGLQNVTQIFWEQSQAKGAVPNAAKYVDQSYLRAALK"
     gene            1786..2322
                     /locus_tag="VMZ02_11440"
     CDS             1786..2322
                     /locus_tag="VMZ02_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HUR72595.1"
                     /translation="MTHPPKILKTARLRLRKAKLSDAEAIFREYAKDPEVTKYVSWRA
                     HRNVEETREYMRMCSMAWDIGRAFHWVIETQEGKQVIGMMIARVNAEKWELGFVLARA
                     YWGRGYMTEAVKGLIAWALKQQEIFRVWAVCDIDNKASARVMEKAGMRREGTLNRWSV
                     HPNISPEPRDSYCYAITK"
     gene            2389..3402
                     /locus_tag="VMZ02_11445"
     CDS             2389..3402
                     /locus_tag="VMZ02_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72596.1"
                     /translation="MTGRWWCLLLSFVPIIGNYSSYAASDSVGALEKSEIVVAYPQAS
                     GVFTPLFVANEAGLFKKYGLNVKLQQLNPQLSVQSVVSGSADMSVAAGDLVNASLQGA
                     KIKLVGSSMSQLVFQLWAAKEITQVQQLKGKTLAGTTPRSVLEIATREALKRQGLNFD
                     IDYKLIYMQSVPAVLTAVSSGKAAAGALSAPTTLKAKDAGLNMLIDIAKANIPGLPLA
                     YGFTEQFIKQNPNTISAFLKGIAEAVARTKSDPAMAKRAIGKFTQTEDAQVIDDTYDF
                     YAPYFVTHLALRPEQLSTWFGYLDEKEYPAAAKANPKDFYDNSFVEALEKAGFFQKLA
                     QSK"
     gene            3604..>4454
                     /locus_tag="VMZ02_11450"
     CDS             3604..>4454
                     /locus_tag="VMZ02_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HUR72597.1"
                     /translation="MFKNFTVSVAFLFLVLSAHPIFAQERVRVGWAAMTASHTPLWVA
                     QERGLFTKHGLIVEPIFFGAGPPAMQALVAGDLDIVITSAPNVVNPRLGGADLVMILS
                     IIPTFIDHIISAANITTVEQLRGKTGSVNRAGSISDMGMKLSLKRLGIDPDKDVKIVP
                     AGGNPERLASISRGLTQFTIMNEPFIKEAERLGLRDLVNMATLKIPLHGNGVVVREAT
                     IKSRRPMVSRFARAITEALHLVKADKEGTKAVIGKYTRLTDPEGLERTYRNYTSVLLD
                     APYPDPAG"
BASE COUNT          987 a         1201 c         1289 g          977 t
ORIGIN      
        1 ggttttgggg acaacttatc tggaaacttc ggtcaacggc gatgatccca agccgcgcgg
       61 taatcattat gctttggccg cgccgcacgg ggtttaccgc tgccagggcg acgaccgctg
      121 gtgtgtgatt tccgtgcgcg acgacgtcga atggcggaat ttctgccgag tcattggacg
      181 cgtcgaactg atgagcgata gccggtttac ctcgcttcag tcacgcatcg acaaccgagc
      241 tgcgctcgat ggcatcgtca gcgcctggac tatggcgcgc acggcggaag aagtcatgac
      301 cgagtgccaa tcggcgggcg taccggccag cgtcgtgcaa accggtgccg atttgctgaa
      361 agacccgcaa ctgcgccacc gtaattattt ctcggcgttt cccgaatcgc tgatcgggcc
      421 gttcgaaatc ccgcgcagcg gctttgtctt caacggcatg gcggaagatc cgcttgagtt
      481 gccggcgcgt tttggctcgg ataatgacca aatcctcggc gagctgttgg gctatgacca
      541 ggccaccatc gaccagtggc gcgaggaaga agtgctgact taacggctgt tcaaaacgtt
      601 caagccgttc aaaaagttag atcgtttagg cccgacacgg ttgcgtgata ctttctcgct
      661 gatgcgttaa gggagaaaac gatggcctta ccactttctc cgatgctcgc gattctcttt
      721 ttagtttttt taacacccga cacttgcgct caggagcgcg tcaagctgcg catcagttcg
      781 gcggccaaga cgcttggtta cgggccgctg tggatcgcct cgcgcatggg atttttcgac
      841 cggcaaggct tggatgtcga tctcgtggtg attcgcgctt cggatattgg catacaggcg
      901 ctcgctggcg gctccctcga gatagccggc agctcggccg atgcgcccat cgccgcggtt
      961 gaaaagggcc tcgatctggt gatcgttggc gggatcatca acggtttgac tcagtcgatc
     1021 atggcggcaa agaaatttaa aacctacaac gatttgcgcg gcgcgacgtt tggcgccatc
     1081 agtctgacgt ccggtgtgac ctttgcgttg cgccaggtgc ttaagctcaa gggactagag
     1141 tatccgcgcg attacaaact gctcgtcatc ggcggcacgc cgcagaccta tgcggctttg
     1201 gtcgctggct cgatcgatgc agcggcgctg tcgctgcccg tcaactacgc agcggaagag
     1261 cagggtttca acgagatcgg ccgcttcgtc gacatcatgc caaattacca actcgcggcg
     1321 ctgtccgtcc agcgctcgtg gggtgagaaa catcgccccg tgctggtgcg cgtcatgcgc
     1381 gccatggcac aaacaatgcg gtggattcac cagaacaaag agccagcggc caactatctc
     1441 gccaaggaaa tgaatttgaa acccgaccac gcccggcgcg gttgggagtt ctacacgacg
     1501 accaaaatgt ggcatcccga cggtgatctc aatctcgaag gactgcaaaa tgtcacgcaa
     1561 atattctggg aacagagcca ggcgaaaggc gcagtgccga acgcggcgaa atacgtcgat
     1621 cagagttatt tgcgcgcggc tttgaaatag atttatgaag gcaccacgca gcaggcaccc
     1681 ggcaactaga acgccttatc cctactgcta ctgttttact tatctcttga atattttacc
     1741 cggtgaccct aaagttgaaa atcatgtgcg ctggaccttc ctagagtgac acatccaccg
     1801 aagatactta aaaccgcacg tttacgactc agaaaagcca aactatcgga tgccgaggcg
     1861 atttttcgcg agtacgcgaa ggatcccgaa gtaaccaagt acgtcagctg gcgcgctcat
     1921 cgaaatgttg aggagacgcg cgaatacatg cgcatgtgtt cgatggcctg ggacatcggc
     1981 agagcatttc attgggtgat cgagactcaa gaaggcaagc aggttatcgg catgatgatt
     2041 gccagagtga atgccgaaaa atgggaattg ggctttgtct tggcgcgggc gtattggggg
     2101 cgcggttaca tgaccgaagc ggtcaaaggt ctgatcgcct gggcgctcaa gcagcaagaa
     2161 atttttcgcg tctgggcagt gtgcgacatc gacaacaagg cctcggcacg ggtgatggaa
     2221 aaggccggca tgcgccgcga agggactctc aatcgctggt cggtgcatcc taacatcagt
     2281 cccgagccgc gcgattccta ttgctacgcg atcaccaaat agaaccgtaa aacttgcagt
     2341 gctggagttt tgaagggttg gccatagcaa cgcgatccgg aggtttttat gactggtcga
     2401 tggtggtgct tgcttctgag cttcgtccca atcattggga attattcgtc ttacgctgcc
     2461 agcgattccg tcggtgcctt ggaaaaaagc gaaatcgttg tcgcttaccc gcaggcaagc
     2521 ggggtcttta cgccgttgtt tgttgccaac gaggccgggc ttttcaagaa gtacggactc
     2581 aacgttaaac tccaacagct caatccgcag ctctcggttc aatccgtcgt ttccggctcc
     2641 gccgatatgt cggtggcggc aggggatctc gtcaacgctt cgttgcaggg cgccaagatc
     2701 aaactggttg gcagctccat gtcacaactg gtctttcagc tctgggcggc aaaagaaatc
     2761 acccaggtgc agcagctcaa gggcaagacg ctggcgggaa cgacgccgcg ttccgtgtta
     2821 gagatcgcca cccgagaagc gctcaagcgc cagggtttga atttcgatat cgactacaag
     2881 ctcatctata tgcagagcgt gccggcggtc ttgacggcgg tctcgagcgg taaagcggcg
     2941 gcgggcgcat tgtctgcgcc gacgacactg aaagccaaag acgccggtct gaatatgctg
     3001 atcgacatcg ccaaagcgaa tattcccggg ctgcctttgg cgtatggctt caccgagcaa
     3061 ttcatcaaac aaaatcccaa cacgatttca gcgtttctga aaggcatcgc cgaagcggta
     3121 gcgcggacca aaagcgatcc ggcgatggca aagcgcgcca tcggcaagtt cacgcagacc
     3181 gaggatgccc aggtaatcga cgatacttac gatttctacg cgccttattt tgtcactcat
     3241 ctggctctgc ggccggagca gctctccact tggtttggtt acctcgacga gaaagaatat
     3301 cccgcggcgg ccaaggccaa cccaaaagat ttctacgata actcttttgt cgaggcgttg
     3361 gagaaggcag ggttctttca aaaactcgca cagtcgaagt gactgcgggc tcaccgttca
     3421 tgcagctgcg gtgaaaatat gtgtcgggac agccagtcct tattcgtcat accggcgtaa
     3481 gccggtatcc aggggcggcg gatagtgcgg cctgcgttaa agatggattc cggcgtgcgc
     3541 cggaatgacg gatttaatac tatcggacgt gagcgtaagg cgcgtcgtct cgacgaggta
     3601 aatatgttta agaatttcac ggtctcagtt gcttttttat ttctcgtttt atcggcccat
     3661 ccgattttcg ctcaagagcg cgtccgcgtc ggctgggcgg cgatgacggc atcgcacacg
     3721 ccgttgtggg tggcgcagga acgcggcttg tttaccaagc acggcttgat tgtcgagccg
     3781 attttctttg gcgccgggcc gccggcgatg caggcgctgg tggccggtga tttggatatt
     3841 gtcattactt cggcgcccaa tgtggtcaat ccacgcttgg gcggcgccga tctggtgatg
     3901 atcctgtcga tcatcccgac tttcatcgac catattatca gcgccgcgaa tatcacgaca
     3961 gtcgaacagc tacgcggcaa gaccgggtcg gtcaatcgcg ccggcagcat ttccgacatg
     4021 gggatgaagc tttcactcaa acgcttaggc atcgatccgg ataaggacgt caagatcgta
     4081 cccgccggcg gtaatcccga acgattggcg tcgatctcaa gagggctgac gcagtttacg
     4141 attatgaacg aaccatttat caaagaagcc gaacggcttg gcttgcgcga tctggtcaat
     4201 atggcgacct taaagattcc gctccatggc aacggcgtcg tcgttcgcga ggcaaccatc
     4261 aagtcccggc gtccgatggt gagccggttt gcccgggcga tcaccgaagc gctgcacttg
     4321 gtcaaagcgg ataaggaagg caccaaggcg gtcatcggta aatatacccg gttaacggac
     4381 cccgaaggat tagaacgaac ctatcgcaat tacacttcgg tattgcttga cgcgccctat
     4441 ccggatcccg ccgg
//
LOCUS       DATLCH010000381         3278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_10386863, whole genome shotgun sequence.
ACCESSION   DATLCH010000381 DATLCH010000000
VERSION     DATLCH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10386863"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /gene="scpB"
                     /locus_tag="VMZ02_11455"
     CDS             complement(<1..517)
                     /gene="scpB"
                     /locus_tag="VMZ02_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HUR72598.1"
                     /translation="MEREEIKSILESLLFVADGPLTVQRFVEVLDGVDKETIHALILE
                     LQSELEAPSRGLQLVEVAGGYQLRTAKVNADWVKKFLGGRPARMGRATLETLAIIAYR
                     QPITKAEIESIRGVDVDGVITTLLERNLVRAVARKDVPGRPFLYGTTGEFLQLFNLKD
                     LTELPTLKETDE"
     gene            complement(507..1283)
                     /locus_tag="VMZ02_11460"
     CDS             complement(507..1283)
                     /locus_tag="VMZ02_11460"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HUR72599.1"
                     /translation="MSTHVETEIFEGPLDLLLHLIKKNEVSITDIPIATITEQYLATL
                     DLLQSLSLDVAGEFLVMASTLIHIKSRMLLPAGDDVGDDDEDQGLDPRDELVRRLLEY
                     QRYKEAAQDLDQREVLSRDVFTRSAAPNEEAGPRGFREVSVFELLAALKRIIDRLPKD
                     TFHDVTLEKITVREKMTHLLDVLRAQGSVLFEAFFATANSRMEIIVTFLAMLELVKVR
                     AIRIVQEQLAGPITIEAAAVMDSVSEQAVLEQVEEEPYGA"
     gene            complement(1280..2257)
                     /gene="trpS"
                     /locus_tag="VMZ02_11465"
     CDS             complement(1280..2257)
                     /gene="trpS"
                     /locus_tag="VMZ02_11465"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HUR72600.1"
                     /translation="MDTIVSGMRPTGRLHLGHFFGALINWLKLQEQYHCYFFVADWHA
                     LTTDYATPQGINQNTIEMVIDWLSVGLDPAKAVVFRQSRIKEHAELHLLYSMIVPVPW
                     LERNPTYKDQIKEMVGKDLSTYGFLGYPVLQAADITIYKATKVPVGVDQAPHVELTRE
                     IVRRFNQVYAPIFPEPEVLLTETQKLPGLDGRKMSKSYGNAVLLSDTPKEIDQKLSKM
                     MTDPARVRRTDPGEPEKCPAFQFHKIYCTPEEIAEVSHGCRTAGIGCLDCKKVMIKHV
                     VAQLAPISEKRADLAQRPKDVEDILTAGNKVAQQKASETMTEVRETLGL"
     gene            complement(2403..3056)
                     /locus_tag="VMZ02_11470"
     CDS             complement(2403..3056)
                     /locus_tag="VMZ02_11470"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HUR72601.1"
                     /translation="MEDTIQKIAVWAIPVLIAIVFHEVAHGWVANSLGDPTAKNLGRL
                     TLNPIAHIDLFGTILVPAMLILAHSPFVFGYAKPVPVNFHNLRNPKRDMVWVALAGPV
                     MNLLLALGFVMIWRFLLPQSRAGLGGSSYLTPLVLMAQGGLLINISLAVFNLFPLPPL
                     DGGRVLVGLLPEPWSSKMAGIERFGMLALIGLLYTGVIDRVIDAPTEFLLKLYLRVF"
BASE COUNT          781 a          940 c          829 g          728 t
ORIGIN      
        1 tctcgtcggt ctccttaagt gtcggcagtt cggttaaatc tttgagatta aacaactgga
       61 gaaactcacc ggtagtgcca tatagaaaag gccttcccgg cacatccttg cgcgctaccg
      121 cgcgaacgag attgcgctcc aaaagcgtcg tgattacgcc gtcgacgtcg acaccgcgga
      181 tcgattcgat ctccgctttg gtaatcggct gccggtaggc aatgatcgcc aacgtctcca
      241 gcgtcgcccg acccatgcgc gccggccggc cgccgagaaa cttttttacc cagtcggcat
      301 tgaccttggc agtgcgcagt tgatagccac cggccacttc gaccagttgc agcccgcggc
      361 tcggcgcttc gagctcgctc tgcaactcca aaatcagcgc gtgaatcgtc tccttgtcga
      421 caccatccaa aacttcgacg aaacgttgca ccgttagcgg tccgtcggcg acaaacagca
      481 aactttctag gatcgatttg atttcctcac gctccataag gctcttcctc gacctgttcg
      541 agcaccgctt gttcgctgac actatccatg accgccgctg cttcgatggt gatcggtccg
      601 gctagctgtt cctgaacgat gcgaatggcg cgaactttta ccagctccaa catggctaaa
      661 aaggtcacaa tgatctccat gcgcgaattc gccgtggcga aaaaagcctc gaacaagaca
      721 gatccctgcg cccggaggac atcgagcagg tgcgtcatct tctcgcgcac ggtaattttc
      781 tccagagtca cgtcatgaaa agtatctttg ggcagacgat cgatgatccg cttcaatgct
      841 gccaggagct cgaacaccga aacttcgcga aaaccacgcg gcccggcctc ttcattgggt
      901 gccgccgaac gggtgaacac atcccggctc aggacctcgc gctgatcgag atcctgggcc
      961 gcttccttat agcgctggta ttcgagcaat cggcgcacca actcatcgcg tggatcgagc
     1021 ccttgatcct cgtcgtcgtc cccgacgtcg tcgccagccg gcagaagcat gcgcgacttg
     1081 atgtgaatca gtgtcgaggc catcaccaga aattctccgg ccacgtcgag actcaaagat
     1141 tgcaacagat cgagggtcgc aagatattgc tcggtgatcg tcgcgatcgg gatatcggtg
     1201 atactgactt catttttctt gatcagatga agcagtaggt ccagaggacc ttcgaagatt
     1261 tccgtttcga cgtgcgtact cacagtccca gagtctccct gacttcagtc atggtttcac
     1321 tcgctttctg ctgcgccacc ttattgccgg cggttaatat atcttcgacg tccttgggcc
     1381 gctgcgccag gtccgcgcgc ttctcgctga tcggcgccaa ctgagccacc acatgtttga
     1441 tcataacttt tttgcaatcg aggcagccga tccccgcggt ccggcagccg tgcgacactt
     1501 cggcaatctc ttccggcgtg caataaattt tgtgaaactg aaacgccgga catttctccg
     1561 gttcccccgg atcggtgcgc cgcacccgcg ccggatccgt catcattttg ctcaacttct
     1621 gatcgatctc tttgggcgtg tcggagagca gcaccgcatt accgtagctc ttgctcatct
     1681 tacggccatc gagtcccggt agtttctgag tttcggtgag caatacttca ggctcgggaa
     1741 agatcggcgc ataaacttga ttgaaacggc gaacgatctc gcgggtcaac tccacatggg
     1801 gtgcctgatc gacgcccact gggaccttgg tggctttgta aatcgtaatg tccgcagctt
     1861 gcagcaccgg ataaccgaga aagccgtaag tcgataaatc cttaccgacc atttctttaa
     1921 tctgatcctt ataggttggg ttccgttcca accagggtac cgggacgatc atcgaataga
     1981 gcaaatgcaa ctcggcatgc tccttgatgc gcgactggcg aaacaccacc gccttcgccg
     2041 gatctaggcc gacgcttaac cagtcgatga ccatctcgat ggtattctga ttgatcccct
     2101 gcggcgtggc atagtcggtg gtcagcgcgt gccaatcggc aacaaaaaaa tagcaatgat
     2161 actgctcctg gagcttgagc cagttgatta aagcgccgaa gaagtgtccc agatgaagcc
     2221 gtccggtggg gcgcatgccg ctaacgatgg tgtccatgtc tcctcaagta agtattgcgc
     2281 tacaagccga gatattctga ttggatcgca tgcaattcag cttgcttttc ctcagtgcag
     2341 cgaatgcggg aatctggaat agacctgtgt atttctcaag gttgaatccc gtgtgcgctg
     2401 gattagaaga ccctcaagta gagtttcaac aagaattctg ttggcgcatc gatgacgcga
     2461 tcgattacac ccgtatacaa cagaccgatc aaggccaaca tgccgaagcg ctcgatgccg
     2521 gccattttgg atgaccaggg ctcgggcaac aatccgacaa gaactctgcc gccatccagt
     2581 ggcggtaagg gaaaaagatt gaacaccgcc aaggagatgt tgatcaacag accgccttgc
     2641 gccatcaaga ccagcggagt caggtatgaa gaaccgccca aaccggccct gctttgcggc
     2701 agcaagaagc gccagatcat cacgaaaccg agcgccagca ataaattcat caccggcccg
     2761 gccaaggcga cccagaccat atcgcgcttg gggttgcgca aattatggaa gttaaccggc
     2821 accggcttgg cgtagccaaa gacaaatggc gaatgggcca aaatcagcat cgccggaacc
     2881 aggatcgtgc cgaacaaatc gatatgcgcg atcggattga gcgtcaaacg acctaagttt
     2941 ttggccgtcg gatcgcccaa actattagcc acccatccgt gagcgacttc atgaaatact
     3001 atagctataa gaaccggaat cgcccaaacg gcaattttct gaatggtgtc ttccaataat
     3061 ttctcgcggg atcctacacc tatagaaata cggtatgaac cgcgaaaaca caaggcgaga
     3121 taagggatac gatcggtgaa cgaaagtcac agtacatcag acacactccc cacatctatg
     3181 aacgaagaat cgccggtctt tcataaaagt cttcgactat ggcgaaagtc tcggcttgca
     3241 aaggcgcacc ttttcctacc cttgagtttc aacaatga
//
LOCUS       DATLCH010000382         8641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8168818, whole genome shotgun sequence.
ACCESSION   DATLCH010000382 DATLCH010000000
VERSION     DATLCH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8168818"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(476..895)
                     /locus_tag="VMZ02_11475"
     CDS             complement(476..895)
                     /locus_tag="VMZ02_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR72602.1"
                     /translation="MAPFVHQSPLMQRTNKKVLIVDRNDAFRSSLGTFLKGLGHDVFE
                     GVTGQEAIDKAGSIRPDLIMMEVQLPGLNGDDVTVRLKSNRSTRNIPVVINTGWTTAC
                     NIEERVERARVAGAAEVLYKPLQFPMLRNILRSYLMV"
     gene            1152..1448
                     /locus_tag="VMZ02_11480"
     CDS             1152..1448
                     /locus_tag="VMZ02_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72603.1"
                     /translation="MNRFVSTPVAAVLLSFVIGCAGVTRNPPEVASQEVARGEYCHKK
                     LPAVGPAAPTMVQPAAGDIIDYYGPCDGPSLTEQRQHQRRFESFRFGRDYMDEG"
     gene            1758..2150
                     /locus_tag="VMZ02_11485"
     CDS             1758..2150
                     /locus_tag="VMZ02_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HUR72604.1"
                     /translation="MPYKVLIIEDNTDCRELFAMLVRHLGCDVLQAHDGELGVQKALN
                     EKPDLIILDIAMPNMNGMEALSCLRESAITKSTPIVVYTAWIGENYREAALKSGAHEF
                     ITKPISFGELQKVLLRYIPAPVNSAEAH"
     gene            2353..3060
                     /locus_tag="VMZ02_11490"
     CDS             2353..3060
                     /locus_tag="VMZ02_11490"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HUR72605.1"
                     /translation="MKPQEWRQQLGECVRDLFRSPEMEQFYNVKLTQKRAQIYLLQLS
                     LYVRKRRDFWPQVAANCPEFEVKQRIMSHEYEELIEDEHSKHGHLDLIFRQGKEVGLS
                     VEDIMDAEPLPTTTSAVYAWWWIARTRSWQESVAASTIAEWTNDDRLLGDLGGGNCTR
                     LYQNWKRDLNLSDAQMPNFTAHSKADVKHSDMFIDVLEKYVVPGREQDVLRTAKESMD
                     IHRAYFGGMAQAMEKTN"
     gene            3074..4072
                     /locus_tag="VMZ02_11495"
     CDS             3074..4072
                     /locus_tag="VMZ02_11495"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HUR72606.1"
                     /translation="MKLEELDSQLEKNQLSGFWRTRVPSHAAEAPYLWKWEPLLDGLL
                     KASETIGIDQAERRAIRLVSPHLPIKSTSHTLQITFSIVNPGEIAKAHRHNMAAIRFV
                     VQGKGAYTAVNGEKFFMEEGDLILTPNWTWHDHHNESEDPIIWLDGLDGPLIQSLNVL
                     FFEDAEKPEQEITRQTGESLARMGFARPTKQEEIHRRGVPFRYSFKDTYKALQAMGPA
                     DRDPYDGILLRYINPATGGYTYPTMSCEIQLFKAKETTKMHRHTSSALYHVVQGQGRT
                     KVGDTYLDWRKGDTFVVPLWQWHAHENLGNDEAVVFSLSDRPVMESLQLYREETSI"
     gene            4111..5115
                     /locus_tag="VMZ02_11500"
     CDS             4111..5115
                     /locus_tag="VMZ02_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HUR72607.1"
                     /translation="MAATVKIRLASRAYDGTLPILRRQLSIAGFDFDVTETEDVPGMF
                     AGMYKGDYDVSEMSLGELISYTSRGKAEFVGIPVFPSRMFRHGFIFCRKDSGINRPAD
                     LSGRKIGFLRWVQTAAIWMRGMLIDEYGVSAKNSQWYVASMHHWDDHDPGATVVPRDG
                     SVIRLIENSGKNTSERACRALFDSQVDALGVTESQLPTLLANTDVKRLFENPREVEAS
                     YFRNCKILPIMHVLAVQKQLIERYPALPEQLFRLYSDAKRWAQRWRRAIPSLVEAWPT
                     HYLNDEQEIFQTDPWAYGLEANRHVLEKFFAYCHDQGISGRNIAIEEIFHPSTFKLSE
                     "
     gene            5145..6638
                     /locus_tag="VMZ02_11505"
     CDS             5145..6638
                     /locus_tag="VMZ02_11505"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HUR72608.1"
                     /translation="MSTASAINANANGNIDLRDWLKEVKRLEQLETVSGAHWDLELGA
                     LTEIILERIATPPAVIFDNIPGSEPNRRVLVNMLETLERTALTLNLPLDLKIIPLIDA
                     LRGRLRAMQPIPANVVKTGPVFENIAHGDQIDLTKFPVPRWHAGDGGRYLGTAHLVVT
                     RDPETAVENIGCYRVMLQDKDKVGLYISPGKHGKIHYEKAMKAGKNFPVVMAFGQHPL
                     LYIAASQAVPFGVSEYDWAGGLIGQPVDVIEGPVTGLRFPANSEIVIEGEIVPGESMQ
                     EGPFGEWPGYYASARRAEPFVRVKALYHRNDPIITGAAPFKPTIHGMYRSCLRAATVW
                     NGMEQAGVPDIKGVYLPPPAQRFMIVVAITQRYPGHAKQAALVASQCHAGAYLGRYVI
                     VVDDDIDITSTDEVMWAICTRTDPATSVDLLRRCWSGPLDPIIQPGQKGFNSRMIIEA
                     VRPWEWRDKFPATSAITEETRHQYASKWQNHLAQLQARHTQARYRGD"
     gene            6985..7203
                     /locus_tag="VMZ02_11510"
     CDS             6985..7203
                     /locus_tag="VMZ02_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HUR72609.1"
                     /translation="MEEIMTPSEVASLLKIHLKTVYKLAEKGIIPGNRIGRSWRFSRG
                     DVLELVSTKSINLSEPSDGDLKIAANGK"
     gene            7388..7741
                     /locus_tag="VMZ02_11515"
     CDS             7388..7741
                     /locus_tag="VMZ02_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72610.1"
                     /translation="MDLQYQRLMQALREMQAQIDQRVRPAVQLMVQNEIDYLRAQSEE
                     KIAALKDCLSQIDQSILQCLAGLDEYQKCYVDLAMLNTKLTDLGVSPEPLPENIPLDQ
                     LSETISARIANLSRY"
     gene            complement(7841..7915)
                     /locus_tag="VMZ02_11520"
     tRNA            complement(7841..7915)
                     /locus_tag="VMZ02_11520"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7880..7882),aa:Arg,seq:ccg)
BASE COUNT         2014 a         2262 c         2366 g         1999 t
ORIGIN      
        1 atggctaatg agcgcgtgat cgggtacgac ggcagaatct ccgccgcgaa atcgcagctg
       61 gaagtctttg tcattcatac cgacgaagag gcaatcgtcg cgcaggaaac cacacggctg
      121 ataagagcta ctgcggcaaa actttagaaa tttttcgcgt tgatcgcgca ccctggaagt
      181 caccgtcggt tatgcgcgat accgtgacgt cacgcctcca aattcgcaat cctgccaagt
      241 cgacgacgaa gccaaagccc gaagccagat actgtccttc gccggaaatg tttttctatg
      301 aacgcgtgtc taaggtttag atagactcgg cgaatggcaa gaaagatctc agccgcgttt
      361 ggcgtctcgg ttggtgtatc gagcgcaact gctgcatagg gaggcaaagc gaaaagtggc
      421 tgatcagatg ccgcaggcct tcgcaggctt gcgcatggtt tttttcaagg gagggctaga
      481 ccattaagta gctgcgcaaa atattgcgca gcatgggaaa ctgcagcggc ttgtaaagaa
      541 cctcggcagc accggcgaca cgagcgcgct caacgcgctc ttcgatgttg cacgccgtgg
      601 tccacccggt gttgatgacg acgggaatat tacgagtgct ccgattcgac ttgagccgca
      661 cagttacgtc gtcgccattt aatcccggta actggacttc catcattatt agatccggtc
      721 gaatgctgcc ggccttatcg atggcctcct gccccgtgac accttcaaag acatcatgac
      781 ccaagccttt gagaaacgtg ccgagagagc tgcgaaatgc gtcattacga tcgacaatca
      841 atactttttt gttggtgcgc tgcatcaacg gcgactggtg cacgaaaggt gccaccgtct
      901 atcgcctccg tccgtaagca gtttttgtcg cgccgttgcc ttccagagat aactgactaa
      961 gcagttgtgt gatgtctgat ttgatctgaa aagtagaaac ttctttctac cggttaattt
     1021 tacccggtcc aggcaagacg caatggcgat gcgagataac gcctcaaatc aacaaatagt
     1081 gatttgacaa gcctcgttaa gggtggataa tcatttcctg gttaacttaa aggggcaacg
     1141 aggagatgtt tatgaaccgt ttcgtttcaa cgcctgtggc tgccgttcta ttatcgtttg
     1201 ttattggttg tgccggtgtc acacggaacc cccctgaggt tgccagtcag gaggtagcga
     1261 gaggggagta ttgccacaag aaattgccgg ccgtgggtcc ggcggcgccg accatggtac
     1321 agccggcagc tggggacata atcgactatt atggtccctg tgacggccca tcgctcaccg
     1381 agcaacgcca gcatcagaga cgattcgagt cgtttcgttt cggcagagac tacatggacg
     1441 aaggataaga tcctatttac caaccaaagc gcttggaccc gttgttggtt cgctcgaccc
     1501 cgcatgcttg tcagatctcg gccaatcacg taacaaacag ctattcacgg ccgaaggtcc
     1561 cggcgtaaca agattttgag atattgccaa atcaatgatt gccgatgcgt ctgcgcccgt
     1621 tactgtcagc tcaagagctg ataatcttcc tggtaagctc gtgaacctta ccggcagtgc
     1681 atacctgctg cccgtaatga gagtcgatac aggcttggca caggagctgc tttttaccgc
     1741 ggtctggagt cgggtcgatg ccatataaag ttctgatcat tgaagacaac accgattgcc
     1801 gcgagctgtt cgcgatgctg gtgcggcact tgggatgcga cgttctgcag gcccatgacg
     1861 gcgaactcgg cgtgcaaaaa gccttaaacg agaaacccga tttgatcatc ctcgatatcg
     1921 ccatgccgaa catgaatggc atggaggcgc tctcgtgttt gcgagagagc gcgataacca
     1981 aaagcacacc gattgttgtc tacacggctt ggattggcga gaactatcgc gaagcggcgc
     2041 tgaaatcggg tgcccacgaa tttatcacca agcccatctc gtttggcgaa ctacaaaaag
     2101 ttttgttgcg ttacattcct gcaccggtca attccgccga ggcgcactag ccgccacgtg
     2161 atccgttccc gcgtgtctcg agccatcaat agcgccttcc ttcgtgctgt ttttccgtgc
     2221 tagcttgaca agccttcggt cgatcagcta ggtctacaaa agtagaccgc tgaattaatt
     2281 tatcactctc agatcgggtc tgggcagttc tcatacggta ggcttggcac tgatagtaga
     2341 ggaggagtaa tcatgaaacc acaagaatgg cgccaacaat tgggtgaatg cgtgcgcgat
     2401 ctgttccgct ccccggagat ggaacaattt tataatgtga agttgacgca aaagcgcgcg
     2461 cagatttatc ttttgcaact tagtctgtac gttcgcaagc ggcgtgattt ttggccgcag
     2521 gtggcggcga actgtccaga gttcgaggtc aaacagcgca tcatgtcgca cgaatatgaa
     2581 gagttgatcg aggacgaaca ttccaaacat ggccatttgg atctaatctt tcgtcagggc
     2641 aaggaagttg gcttgagcgt cgaggatatt atggatgctg agccgctgcc gacgacgacg
     2701 tcggcggttt acgcgtggtg gtggattgct cgtactcgca gttggcaaga atcggtggcg
     2761 gcctccacaa tcgctgagtg gaccaacgac gatcgcttgc tcggcgatct tggcggcggc
     2821 aattgcaccc gcctctacca aaactggaaa cgtgatctca atttgtctga cgcgcaaatg
     2881 cccaatttca cggcgcacag caaggcggac gtgaagcatt ccgatatgtt tatcgatgta
     2941 ttggaaaaat acgtcgtgcc tggccgcgag caagacgttc tcaggaccgc gaaggagtcg
     3001 atggacattc atcgcgctta tttcggcggc atggcgcaag cgatggagaa aactaattga
     3061 gcttggagcc gcaatgaagc tcgaagaact cgatagccaa cttgaaaaga accagctttc
     3121 tggattctgg cgcacacgtg tgccttcgca cgcggctgag gctccctatc tttggaagtg
     3181 ggagccgcta cttgacgggt tgctcaaggc aagcgagacc atcggcatcg accaggcaga
     3241 gcggcgcgct attcgtttgg taagcccgca tctgccgatt aaatcgacat ctcatacgct
     3301 ccagattacc ttctcgatcg tcaatcccgg cgaaatcgcc aaagcgcatc gccataacat
     3361 ggcggcgatc cggttcgtcg ttcagggcaa gggtgcttac acggcggtga acggcgaaaa
     3421 gtttttcatg gaagagggcg atctgatctt gacacccaac tggacttggc atgatcacca
     3481 taacgagtcg gaggacccga ttatctggct cgacggtctc gacgggccgc tgatccaatc
     3541 actcaacgtt ctgttcttcg aagatgccga aaagccggag caagagatta ccaggcaaac
     3601 cggcgaatcg cttgcgcgga tgggttttgc tcggccgacg aagcaagaag aaatacaccg
     3661 gcgcggcgtg ccgtttcgct actcgttcaa ggatacttac aaggcgctcc aggcgatggg
     3721 acccgccgat cgcgatccgt acgacggcat cttgctgcgc tacatcaatc cggctaccgg
     3781 tggttatacg tatccgacga tgtcctgtga gattcagctc ttcaaagcca aggaaacgac
     3841 caagatgcac cggcatacca gttcggcgct gtatcacgtt gtccaaggcc aaggccgcac
     3901 taaagtcggc gatacctatt tggattggcg caaaggcgac acgtttgtgg ttccgctttg
     3961 gcaatggcac gcccacgaaa atcttggcaa cgatgaagcg gttgtgtttt ccttgagcga
     4021 tcgaccggta atggaatcac tgcaactcta tcgcgaagaa actagcatct agcttttact
     4081 tccttccaac gccgtttttt agggtcaatc atggcagcaa ctgtaaaaat tcgccttgca
     4141 agccgcgcct atgacggtac cttgccgatc ctgcgccggc aattgagcat tgccggtttt
     4201 gatttcgacg taaccgagac cgaggatgta cccggtatgt tcgccggcat gtacaaagga
     4261 gactatgatg tctccgaaat gtcgctcggc gaattgatct cctacacttc gcgcggcaaa
     4321 gccgagtttg tcggcatccc ggtgtttccg tcgcgaatgt ttcgccatgg atttatcttc
     4381 tgccgaaaag actcgggaat taaccggccc gccgatctga gcggtcgcaa aatcggattt
     4441 ttgcgttggg tgcagaccgc ggcaatctgg atgcgcggta tgctgatcga tgaatacggc
     4501 gtctcggcca agaattccca atggtatgtc gcttcaatgc atcattggga cgatcacgat
     4561 ccaggggcga cagtggtgcc gcgtgatggc tcggttattc gtttgatcga aaacagcggc
     4621 aagaatactt ccgagcgggc gtgccgggcc ttgttcgaca gtcaggtcga tgctcttggc
     4681 gttaccgaat cccaattgcc gactctgctg gcaaacaccg atgtcaaaag gctgttcgaa
     4741 aatccccggg aagtggaagc gagctatttc cgcaactgca agatcctacc gatcatgcac
     4801 gtgctggcgg tgcagaagca attaatcgag cgatacccag cattaccgga gcagctattt
     4861 cgcctttaca gtgacgctaa acgttgggcg cagcgctggc gccgcgcgat cccgagtctc
     4921 gtcgaagctt ggcccactca ttacctcaac gacgaacagg agattttcca aaccgatcca
     4981 tgggcgtacg gcctggaagc caatcgccat gtgctggaga aattttttgc ctattgccat
     5041 gaccaaggca tatccggccg caacatcgcc atcgaagaga tatttcatcc gagtacgttt
     5101 aagctgagcg agtaatttcg ccatcgatcc tgaagggagt tctcatgtcc acggcgtccg
     5161 caatcaacgc caatgcgaat ggcaatatcg atctgcgcga ttggctcaag gaggtgaagc
     5221 ggctggaaca attggaaact gtatcgggcg cccattggga tttagaactg ggcgcgctga
     5281 ccgagatcat tctcgaacgt atcgccacgc cacctgcggt gattttcgac aatatcccag
     5341 gttcggaacc gaatcggagg gtattggtca acatgttgga aacgctggag cgcaccgcgt
     5401 taacgttaaa tctgccattg gatttgaaga tcattccact gatcgatgct cttcgcgggc
     5461 ggctgcgggc catgcagccg attccagcta acgtcgtcaa gactggaccg gtgttcgaaa
     5521 atatcgccca tggcgaccag atcgatctga ctaaattccc tgtgccgcgc tggcatgcgg
     5581 gggacggcgg gcgctatctc ggaaccgctc atttggttgt gacccgtgat ccggaaaccg
     5641 ctgtcgagaa tatcggctgc tatcgggtca tgttgcagga caaggataaa gtcggtctgt
     5701 acatctcgcc cggcaagcat ggcaagattc actacgagaa ggccatgaag gccggcaaaa
     5761 attttccggt ggtgatggca ttcggacaac atcctttgct ctacatcgcc gcctcgcagg
     5821 cagtgccttt cggggtgagc gagtatgatt gggccggcgg actcatcgga cagccggtgg
     5881 acgtgatcga gggcccggta accggtttac gattcccagc gaattccgag atcgtcattg
     5941 aaggtgagat cgttcccggt gagtcgatgc aggaaggtcc tttcggtgag tggcccggat
     6001 attatgccag cgcccggcgc gccgagccgt tcgtgcgtgt caaggcgctc taccatcgca
     6061 acgatcccat cattaccggt gccgcacctt tcaaaccgac aattcacgga atgtatcgct
     6121 catgtctgcg cgctgcgact gtgtggaacg gcatggagca agccggtgtt cccgacatca
     6181 aaggcgtcta tttgccgccg ccggcgcagc gctttatgat tgtcgtcgct atcactcagc
     6241 gttatccagg acatgccaag caagctgccc tggtggccag tcaatgccac gctggcgctt
     6301 acctcggccg ttacgtgatc gtcgtcgacg acgacatcga cattaccagt acggatgaag
     6361 tgatgtgggc aatctgcact cgcaccgatc cggcaacgtc ggttgacctg ctgcgacgct
     6421 gttggagcgg tccgctcgat ccaatcattc aaccgggcca gaagggtttt aactcgcgca
     6481 tgatcatcga ggcggtaagg ccgtgggagt ggcgcgacaa attccccgct accagtgcta
     6541 tcaccgagga gacgcgtcac cagtatgcgt caaaatggca gaaccatctc gcacagcttc
     6601 aggcgcgcca tacgcaggcg cgctatcgcg gcgactgagg tgacaattca gtacgctgcg
     6661 accgttgctc agtgcctcca agtgctgcgc gactgacgtt ggtaaaggga cctttcgttg
     6721 attctgtccg agtgccaatt ctggttttcc cttacggtaa atgacgctgg gactgggcga
     6781 aaagcgcatt gattgagcaa tattgtccat gattgctgaa cccgcctgac ctgtctcggc
     6841 aatcgccgtg ttggggaagt ctttggaaat atcaggcttt atccgccgtc cgcagtgtca
     6901 attggcaatt ggcatgactc gtgctgatgc taaccccgac taaagttccg aacggattct
     6961 gacaaataaa aggagatact agtgatggaa gaaatcatga caccctcgga agtggcttcg
     7021 ctgctgaaga ttcatctcaa gacggtatac aagcttgccg aaaagggcat tattcctggt
     7081 aaccgcatcg gccgcagctg gcgctttagc cgtggtgatg tgttggaatt agtgtcgacg
     7141 aagtcgatta acctgtccga gccgagcgac ggcgatttga aaatcgctgc aaacggtaaa
     7201 taatttcttc gagttcagcg tgcgagttcg ctcgatgaat cacgatccaa tccggttctc
     7261 ggaagaacgc aataagatgt tggaacaaat cagcagcatt aaatctacgc ttgtttcgtt
     7321 tcacggcgat ccgccatcca tggcggcttt gaactccgcc acgaattctt ctcctgacgc
     7381 aacgttgatg gatcttcaat accagcggct tatgcaagcg ctgcgagaaa tgcaggcaca
     7441 gatcgaccaa cgcgtgcgtc ccgccgtgca attgatggta caaaacgaaa tcgattactt
     7501 gcgcgctcag tccgaggaaa aaatagcggc gttaaaggat tgtctcagcc aaatagatca
     7561 gagtattttg cagtgcttgg caggtctaga cgaatatcag aaatgttatg tggaccttgc
     7621 aatgctcaac acgaaattga cagaccttgg cgtctcgcct gaaccactcc ccgaaaatat
     7681 tccgctcgat caattgagcg aaaccatcag cgcccggata gccaatctaa gtcggtacta
     7741 ggtttcacaa tctccggtgc cgcgcaaatc tttccctgtg cagcccgtgt ggcaccttac
     7801 gatatgctag cagtagattg cggcaatcaa ttctgaacgt tggtgcgccc gacaggaatc
     7861 gaacctgtac ttaaggcttc ggaggccttc gtgatatcct tttcactacg ggcgcgtgga
     7921 aaattttcta tcatcgggtc ggcggtcagt caacgcgaag gtctcatggc agctgcgatg
     7981 cgcgccattc gcgcgtgaat gaggcgtttg tcgtcagact ttctctgcca gtaatttcgt
     8041 agcgctcgca gcgaaaatcg ctgagcatcc cgcctgcttc ggtgacgatc aaattttccg
     8101 gaaccaaatc ccaaggcttc gtttcaattc cccatgattt tcgatacgac ccatccgaca
     8161 tagcccaagt tagggctacg gagcagttgg atgccgtggc agcggatcaa aaaactgtga
     8221 agtaactcag atattaatcc gcgttgatca aataattcac ggcggaacag cggctgccct
     8281 ggccattgag cgttcgccgt gatctttgat cgcctgaaga ttcaagatta ccgtgttcgc
     8341 attggcggtc ggtatgggtc ataacatcga tcgcgccttt gtaggctatg gtcgtcggtc
     8401 ctgtcggttg gggcgaatgt tcttaccgcc gcgccggccg cttctggcac gacgtcggcg
     8461 atagaagatt gcgtcatggt caccggagtt tcgcaattat caggcagcta tgttagctaa
     8521 tggcgcgtga agattcgagc gccggcgaaa atcaatttga ggttgcgcat cgtcggcaaa
     8581 cgcgccgatg gctatcacga gctcgacacg attatggtgc cagtgtcgct ctacgatatt
     8641 a
//
LOCUS       DATLCH010000383         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_8170439, whole genome shotgun sequence.
ACCESSION   DATLCH010000383 DATLCH010000000
VERSION     DATLCH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8170439"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VMZ02_11525"
     CDS             <1..376
                     /locus_tag="VMZ02_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72611.1"
                     /translation="GENIKYPMLTGIYAEALMHGDPAARKRIRDAVRLADLMAQHSHT
                     LSEIHFEAADRLVVYPTKFPVAWRLGWGDWEEKIARLERLLELWKGNETRLASLDVSF
                     RDQVVARVRGKRSEGPDILTGR"
     gene            383..1609
                     /gene="ftsA"
                     /locus_tag="VMZ02_11530"
     CDS             383..1609
                     /gene="ftsA"
                     /locus_tag="VMZ02_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HUR72612.1"
                     /translation="MAKKNSIVVGLDIGTSKVCAVVGEMTERGVDIIGVGTHSSSGLR
                     KGVVINIESTVTSIKRAVEEAALMAGCEIHTVFAAISGGHIKSFNSHGIVAVKNKEVA
                     QRDLDRVIDAAKAVAIPMDREVLHVLPQDYIIDEQDGIREPLGMSGVRLEAKVHVVTG
                     AVTSVQNIVKCCNRSGLNVAEIVLSPLAAAEAVLTDEERELGVVLVDMGGGTTDITLC
                     HEGTVKHSAILGIGGNHVTNDIAAGLRTPFSDAERIKQRYGFAKAAMAMDEERVEVPS
                     IAGKEAGTVSRQILCEIIEPRLDEIFELVQKEIAKSGFDGSLASGIVVTGGSMLLPGT
                     VEMAERAFGLPVRLGVPTQVGGLVDVIANPIYATAVGLVLHGMKGQERGVARPRDDKM
                     LAKVKHRMSDWLSEFF"
     gene            1649..2881
                     /gene="ftsZ"
                     /locus_tag="VMZ02_11535"
     CDS             1649..2881
                     /gene="ftsZ"
                     /locus_tag="VMZ02_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HUR72613.1"
                     /translation="MFEIVERQNSLTARIKVIGIGGGGGNAVNTMIGSKLTGVDFMVA
                     NTDAQSLEASQAPVRVQLGGLVTKGLGAGANPEIGRRAALEDQEQIREYLEGSDMIFI
                     TAGMGGGTGTGGAPVVARIAREVGALTVGVVTKPFIFEGKKRQRQAEEGIEELKQSVD
                     TLIVIPNQRLLSIAAKTTTMIEAFHKADDVLLQAVRGISDLIITPGLINLDFADVRTV
                     MAEMGLALMGAASASGENRAIEAAQKAISSPLLEDISIQGARGVLINITGGPDLCLHE
                     VNEAASMIQEEAHDDANIIFGAVIDETMADEIRITVIATGFGEEKEERKTAPVQVQVP
                     AAATIANITSAMPKNKKVVHLGTIIDDLDAPTWQRKKPGSEDLETVTLNKNSFSMGAG
                     QEDDDKYDIPTFLRRQMD"
     gene            complement(3223..>3406)
                     /locus_tag="VMZ02_11540"
     CDS             complement(3223..>3406)
                     /locus_tag="VMZ02_11540"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72614.1"
                     /translation="VQANGRVVVNEINTIPGFTRISMYPKMWEASGIPYNKLIDRLIS
                     LALERFRREQRLRVSK"
BASE COUNT          772 a          854 c         1074 g          706 t
ORIGIN      
        1 gggggagaac atcaaatatc ccatgctcac gggcatctat gccgaggcct taatgcacgg
       61 cgacccggcg gcgcgaaagc gcatccgcga tgcggtacgg ctggccgatc tcatggcgca
      121 gcactcgcat acactttcgg agattcattt cgaggcggcg gatcgcttgg tggtctatcc
      181 gacgaaattc ccggtggcgt ggcgtttggg ttggggtgat tgggaggaga agatcgcccg
      241 cttggagcgc ttgctggaat tatggaaggg caatgagaca cggctggcgt cgctggatgt
      301 gagttttcgc gaccaagtcg tggcacgggt gcgaggaaaa agatcagagg ggccggatat
      361 acttaccggt agatagaagg ttatggcgaa gaaaaatagc atagttgttg gcctggatat
      421 cggaacgtcg aaggtgtgcg ccgttgtcgg cgagatgacc gagcgcggtg tcgacattat
      481 cggcgtcggc acccattcgt cgtctggatt gcgcaaaggc gtcgtcatca acatcgagag
      541 tacggtcact tcaatcaaac gggccgtgga agaagcggca ttgatggccg gctgcgagat
      601 tcacacggtg ttcgcagcta tttccggcgg tcatatcaaa tctttcaata gccacggtat
      661 cgtcgcagta aagaataaag aggtggcgca acgcgatctc gatcgggtga tcgatgctgc
      721 caaagccgtg gcgatcccga tggaccgtga agtgctccat gtcttgccgc aggattacat
      781 catcgacgag caggacggca tcagagaacc gctgggcatg tccggtgtcc gtctggaagc
      841 taaagtgcat gtagtgaccg gtgcggtaac gtcggtgcag aacatcgtca agtgctgcaa
      901 tcgtagcggt ctcaatgtgg cggaaattgt gctctcgccg ttagccgccg ccgaggctgt
      961 gctcactgac gaagaacgcg agttgggcgt ggtcctagtc gatatgggcg gcggcaccac
     1021 cgatatcacg ttgtgccacg agggtacggt gaagcactcg gcgatactcg gaatcggcgg
     1081 caaccatgta accaacgata tcgcagccgg tttgcggacg ccgttcagcg atgccgagcg
     1141 catcaagcag cgttatggct tcgccaaagc agcgatggcg atggatgagg agcgggtcga
     1201 agttcctagt attgccggta aagaagcggg aacggtgtcg cggcaaatac tttgcgagat
     1261 cattgaaccg cgtctggacg agatcttcga actggtccaa aaggaaattg ctaaatctgg
     1321 attcgatggt tcgttggcat cgggcatcgt cgtcactggc ggttcgatgt tactacccgg
     1381 caccgtcgag atggccgagc gcgcttttgg cttgccagtg cgcctgggcg taccgaccca
     1441 ggtcggcggg ctggtcgatg tcatcgcgaa tccgatttac gccaccgctg ttgggctcgt
     1501 gttgcatggg atgaaaggtc aggagcgcgg tgtggcgcgc ccgcgcgacg ataaaatgct
     1561 cgccaaggta aagcatcgca tgtcggattg gcttagtgag tttttttaaa attaacaatt
     1621 ttcaagttta gttggaggag gagagaatat gtttgaaata gttgagcggc aaaacagctt
     1681 gaccgcgcga atcaaggtca tcggtatcgg tggtggcggt ggcaacgcgg tcaacaccat
     1741 gatcggctcc aaactgaccg gtgtcgattt catggtggcg aacacggatg cgcaatcttt
     1801 ggaagcgagc caggcgccgg ttcgggtaca actgggcggc ttggtgacca agggtctcgg
     1861 cgcaggcgcc aatccggaga ttggtcggcg cgcggcatta gaggatcaag agcagatccg
     1921 ggagtacttg gaaggctccg acatgatctt tatcaccgcc ggcatgggcg gtggcaccgg
     1981 aaccggcggt gcgcctgtgg ttgcgcgcat cgctcgtgaa gtcggcgccc ttaccgtcgg
     2041 cgtggtgacc aaacctttta tcttcgaagg caagaagcgc caacgccaag cggaagaagg
     2101 gattgaggag cttaagcaga gcgttgatac cttgatcgtc attccgaatc agcgcttgct
     2161 cagcattgcg gctaagacga cgaccatgat cgaagcattt cacaaagccg acgacgtctt
     2221 gttgcaagcg gtacgcggca tttcggattt gatcattacg ccgggcctga tcaatctcga
     2281 ctttgccgat gtgcgcacgg ttatggctga gatgggcttg gcgctcatgg gcgcggcttc
     2341 ggcgagcggc gagaaccgcg ccatcgaagc ggcgcaaaag gcgatttcca gcccattgct
     2401 cgaagacatc tcgattcagg gcgcgcgcgg tgtgttgatc aatatcaccg gcgggccgga
     2461 tctctgcctg cacgaggtca acgaagcggc atcgatgatt caagaagaag ctcatgacga
     2521 tgccaatatc atatttggcg cggtcatcga tgagaccatg gccgacgaga tccgtatcac
     2581 ggttattgca accggcttcg gggaagaaaa agaagaaaga aaaaccgcgc ccgtgcaagt
     2641 gcaggttccc gcggcggcaa ccattgcgaa catcacgagt gccatgccga agaacaaaaa
     2701 agtggttcac ctcggtacga ttatcgatga tctcgacgca ccgacttggc aacgcaaaaa
     2761 gccgggcagc gaggatctcg agacggtgac actcaacaag aacagttttt cgatgggcgc
     2821 cggccaagaa gacgacgaca agtacgatat tccgacgttc ctaagacgac agatggatta
     2881 gccatccggt taaacgctcc tccgaggtgt gccgttcgtc caccgtaccc gctgcggacg
     2941 aacggcagat acctgactgg gagggaaatt ggattgccgg cgcttcgccc gcgctgctgc
     3001 tgcagggtag ccgtgcgcgg aatatcgggc tcgtctacct gggcttggtt gatcgctacc
     3061 cgccgttggc ggcattaggt gccagggaaa aagctcggtt agatggcgaa atcctgaaaa
     3121 gttgcgccac gactttgtcg atggcggcta tcttccagaa gagaagaaat gcacggatcg
     3181 gagatggata gctttgctgc tcgccaccca ggcatagcgg cactatttcg agactcgcaa
     3241 tcgctgttcg cgacgaaacc tttctagggc gagcgaaatc aatcgatcga ttagtttatt
     3301 atagggaatc cccgaggcct cccacatctt cggatacatg ctgatcctgg tgaagccggg
     3361 aatggtgttg atctcgttga ccaccacccg gccatttgct tggacg
//
LOCUS       DATLCH010000384         4254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6611544, whole genome shotgun sequence.
ACCESSION   DATLCH010000384 DATLCH010000000
VERSION     DATLCH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6611544"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VMZ02_11545"
     CDS             complement(<1..511)
                     /locus_tag="VMZ02_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HUR72615.1"
                     /translation="MISRLVGSFRRDPQLALVLIVGVIIAYLSLSPTLMLFYGSFRSK
                     PLGVPGEFTLAHYIHAYSDPLTYQLLLNSFVFAGASAVIATGLAAILAWIAIRTNAPF
                     RKFFEVAAIVPNIFPPVMLSVAWTVLLSPRTGLINRSLMATFDLSAAPFNLYSLWGMV
                     FVESLITTPL"
     gene            complement(614..1636)
                     /locus_tag="VMZ02_11550"
     CDS             complement(614..1636)
                     /locus_tag="VMZ02_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72616.1"
                     /translation="MKTMKRVFALFTVWLGLVSYAAPLLAQPANVEAGKKEGKVVVYG
                     SVPPQSMEGLHQAFKKKYGIEVEYWRGSSTQVSERALTEWRAGKPAYDVIEGNRGVQL
                     IMKDDGLFAKYIPPASEKFPARFKEKDNLITPWRVLPISILYNTEMVKAADLPKTLDD
                     LLNPKWSGKISIPDPTRHTTTAQFLWNLRRKEFKGDKWLDYVKALAKQNPVLVESLAP
                     VTTTVIKGEAPVGITYIKYIKQYKGPVDFVPMERYLTDPNYLSLSAKSPRANAAKLYI
                     DFACSAEGQKEIAEDGEFVLAPGVYPPIKDADKVAPKMVFMENPSEDEFKKLMSGTFR
                     DIFYAK"
     gene            complement(1722..2738)
                     /locus_tag="VMZ02_11555"
     CDS             complement(1722..2738)
                     /locus_tag="VMZ02_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HUR72617.1"
                     /translation="MGQNMRVQIRLVFQLLSIVVLTAQASAQNVSLEAAKKEGKVFVY
                     GTIIPQVMKLIEAGFEAKYGVNIEYWRGDATKVVDRMLTEWRAGKPGFDMVIGARGPL
                     SLAKADGVYTKFTPASAANFPAKFRDKDGQLTAWRITPVGILTNTDLVKPADMPKSLD
                     DFLDPKWQGKISMPDPSRHASTATFLWNLQKFKGDKWMDFVRALAKQKPLLVESYSSV
                     PNAIVRGEAALGISYIQYVPQTKGPIGFAPIDQVLADPSDAAISAKAVNPNAARFLIE
                     YLCSPEGQKKVAETHEFVLSPGVYPAIKGADRIVANMQLLEDPSHEQLQKLLGEFRQL
                     FVAQ"
     gene            complement(2932..3915)
                     /locus_tag="VMZ02_11560"
     CDS             complement(2932..3915)
                     /locus_tag="VMZ02_11560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HUR72618.1"
                     /translation="MRVGLCFDGFYSVQEMIELAHLADDIGMDSIWMSDHLCFRDSMT
                     TSMALLASTDKIKVAAAPASPYSRNPIITAMGIATMDEFAPGRVVGSPGTGNAAALKE
                     AGMESPHPLQTMREYVDILRRYLRGDTVDFKGKMFHINGAKMGFVPAAPIKMYITAVR
                     PRMLQLGGEIGDGVLLSAGCAPGYIRKCADDIAAGAKKSGKTAEQVDVAGFVTAAVSD
                     SAKAAIEANKQFLAYIFRNVHHADNIRLGGGKVDQEGLAAAVGKRDWEAAKKFISDEV
                     VFAHSVAGTPSDCARQLEAFTRGGLDLPIVLPTGTQEARKQVVRMAREIVG"
     gene            complement(3925..>4254)
                     /locus_tag="VMZ02_11565"
     CDS             complement(3925..>4254)
                     /locus_tag="VMZ02_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HUR72619.1"
                     /translation="FHQAGLTTVEARDTGSAYEAIHNSEANFTIKAPASLDHRYIRED
                     VGYGLVPMTDIGKLVGVKTLTMEALITLASTALGVDFRLEGLTLAKMGLEGISAEQFP
                     AILADGF"
BASE COUNT          946 a         1210 c         1124 g          974 t
ORIGIN      
        1 ccagcggcgt ggtaatcaaa ctctcgacaa acaccatgcc ccagagcgaa tataaattga
       61 acggggcggc ggacagatcg aaggttgcca ttagagatcg gttgataagc ccggtgcgcg
      121 gactcaataa caccgtccac gccaccgaca acatgaccgg cggaaaaata tttgggacga
      181 tggccgcgac ctcgaagaat ttgcgaaatg gcgcgttggt gcgaatcgcg atccaagcca
      241 ggattgcggc tagtccggtg gcgataacag ccgaagctcc ggcgaaaaca aacgaattga
      301 gcagtagctg ataggtcagt ggatcgctat acgcgtggat atagtgggcc aaggtgaatt
      361 cgccggggac acccagaggc ttgctgcgaa aactgccata aaacaacatc aaggtcggcg
      421 ataaactcag gtaagcgatg atcacgccga caattagcac caacgccaat tgcgggtcgc
      481 gtcgaaaact gccgactagc cgggaaatca atttccactt cctcgaggtt gcacagttgg
      541 atcgtcaaaa tacaagggcc agccggtgtg gctggccctt ctttattccc taatcgatac
      601 cgcttttcaa gaattacttg gcgtagaaga tatcgcgaaa cgtgccgctc atcagctttt
      661 tgaattcatc ctcgctgggg ttctccatga agaccatctt cggcgccacc ttgtcggcgt
      721 ctttgatcgg tggataaacg ccaggggcaa gaacaaactc accgtcctcg gcgatttcct
      781 tttgaccttc cgcgctgcac gcgaaatcga tgtacagctt ggcggcgttg gcgcgcggcg
      841 attttgcgct caaacttaaa tagttcggat cggtcaaata tctttccatc gggacaaagt
      901 ccaccggccc tttgtattgc ttgatatact tgatgtaggt gatgcctact ggtgcttcac
      961 ccttgatgac cgttgtggtt accggagcca gggattcgac taacacagga ttctgcttgg
     1021 cgagcgcttt tacgtagtcg agccacttat ctcccttgaa ttctttgcgc cgcagattcc
     1081 agagaaattg cgccgtggtt gtatggcggg taggatcggg gatggaaatt tttccgctcc
     1141 actttggatt caacaggtcg tcgagggttt tcggcaagtc cgccgcttta accatttccg
     1201 tgttgtagag gatgctgatc ggcaacacac gccacggtgt gatcaggttg tctttttctt
     1261 tgaaccgagc gggaaacttc tccgaagccg gcggaatgta tttggcgaac aaaccgtcat
     1321 ccttcatgat caactgtacg ccgcggttgc cctcgatgac atcgtaagcc ggcttgccgg
     1381 cgcgccattc ggtcaacgcg cgctcggaca cctgtgtcga cgagccgcgc cagtattcga
     1441 cctcgatgcc gtatttcttt ttgaatgcct ggtgcaaacc ctccatcgac tgaggtggca
     1501 ccgaaccgta aaccacaacc ttgccttcct tttttccggc ctccacattg gctggttgcg
     1561 ccaacagcgg cgccgcatag ctaaccaggc ccagccaaac agtgaacaaa gcgaaaactc
     1621 ttttcatagt cttcattgaa atcctccagc tgaatcgacc agaaagtacc gacgccgcca
     1681 ctttaagtca actacgtcta atatttcgcg cgcctctatg cttactgggc tacgaatagt
     1741 tggcggaatt cgccgagtaa tttttgtaat tgctcgtggc tcggatcttc cagcagctgc
     1801 atgtttgcca cgatccggtc ggcgcctttg atcgccggat agacgcccgg cgacaagacg
     1861 aactcatgag tctcggcgac ctttttctgg ccttcaggcg aacaaagata ttcgatcaag
     1921 aagcgcgccg cgttgggatt gaccgccttg gcgctgatcg ccgcatcgct ggggtcggcc
     1981 agaacctggt cgatgggcgc gaaaccgatg ggccctttcg tttgcggcac gtattgaata
     2041 taggaaatgc ccaaagcggc ctcaccgcga acgatggcgt tgggcactga ggaataggat
     2101 tcaaccagca acggcttttg cttcgccagc gcgcgcacga aatccatcca cttgtcccct
     2161 ttaaactttt gcaagttcca caagaaggtc gcggtgctgg cgtggcgtga cggatcgggc
     2221 atgctgattt tgccttgcca tttggggtcc aaaaaatcgt ctaaactttt gggcatatcg
     2281 gcgggcttca ccaagtcggt gttggtcagg ataccgaccg gggtaatgcg ccaggcagtc
     2341 aactgtccgt ccttgtcgcg aaacttggcc gggaagttcg ccgcgctcgc cggcgtaaac
     2401 ttcgtataca cgccgtcggc tttggccagc gacagcggac cgcgcgcgcc gattaccatg
     2461 tcgaaacccg gcttgccggc gcgccactcg gtcagcatac ggtcgactac cttggtggca
     2521 tcgccgcgcc agtattcgat attgacgccg tacttggcct cgaagccggc ttcgatcaac
     2581 ttcatcacct ggggaatgat cgtgccgtag acaaatacct tgccttcttt cttcgctgct
     2641 tccaggctta cattctgggc agaagcctgc gcggtcagta caacaattga taatagctgg
     2701 aataccaatc tgatctgcac ccgcatattc tgccccatgg aaaatctccc ctgtcgcctc
     2761 tttttcaaag aggggtaatc aatcgcattg aaaagcctcc ccctttgaaa aagggggatc
     2821 gagggggatt tttttttcag catagggaag atgttcatat gtatgcctcg taattgttga
     2881 atcagcacgt aattcctagg atgtttttaa caaatattca cgcccgtaat actaaccgac
     2941 aatctcccgc gccatacgga caacctgttt gcgcgcctct tgtgtgccgg tgggtagaac
     3001 gatgggcaga tcgagtccgc cccgggtgaa cgcttcgagt tgtctggcgc agtcgctcgg
     3061 cgtcccggcc accgagtgag caaataccac ttcgtcgctg atgaattttt tcgccgcttc
     3121 ccagtcgcgt tttccgaccg ccgcggccag tccttcctga tcgactttgc cgccgcccaa
     3181 gcgaatatta tccgcatgat gcacgttgcg aaaaatatac gcgagaaact gcttgttcgc
     3241 ctcgatggcc gctttggcgc tgtccgacac cgccgccgtg acaaagccgg caacatcgac
     3301 ctgctcggcc gttttgccac ttttcttagc acctgcggca atgtcatcgg cacatttgcg
     3361 aatatagccg ggagcacagc cggccgagag caataccccg tcgccgatct cgccgccgag
     3421 ttgcagcatg cgcgggcgca ccgccgtgat atacatctta atcggcgccg ccggcacaaa
     3481 gcccattttg gcgccattga tgtgaaacat cttgccctta aagtcgactg tatcgcccct
     3541 gaggtagcgc cgtaaaatat cgacgtactc gcgcatggtt tgcagcggat gcggcgactc
     3601 catgccggct tctttgagcg cggcggcatt gcccgtgccg gggctgccga cgacacgccc
     3661 cggagcgaac tcatccatgg tcgcgattcc catggcggtg atgatcggat tgcgcgaata
     3721 agggctcgcc ggtgcggccg ccaccttgat cttatcggtc gaagcgagca gcgccatcga
     3781 cgtggtcatg gaatcacgaa aacaaagatg gtcagacatc cagatggaat ccatgccgat
     3841 atcgtcagcc aggtgcgcca actcgatcat ctcttgaacc gaataaaaac cgtcaaagca
     3901 cagtcctacg cgcatagaaa tctcctaaaa tccatcggcg aggatggctg ggaattgttc
     3961 ggctgaaatg ccctccaacc ccattttcgc cagggtcaaa ccttccaagc gaaagtcgac
     4021 gccgagcgcc gtcgacgcca acgtgatcag tgcctccatc gtcagagtct tgacacccac
     4081 cagcttgccg atatcggtca tcggcaccaa accatagccg acatcttcgc ggatgtaacg
     4141 atgatcgagc gaagccggcg ctttgatggt gaaattagct tcgctgttgt gaatcgcttc
     4201 atacgccgat cccgtatcgc gcgcttcaac cgtggtcaga ccagcctgat gaaa
//
LOCUS       DATLCH010000385         3978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Limnocylindrales bacterium isolate
            SMAG_U14319 k141_6670500, whole genome shotgun sequence.
ACCESSION   DATLCH010000385 DATLCH010000000
VERSION     DATLCH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37938798
            Sequence Read Archive: SRR8554898
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Limnocylindrales bacterium (soil metagenome)
  ORGANISM  Candidatus Limnocylindrales bacterium
            Bacteria; Chloroflexota; Candidatus Limnocylindria; Candidatus
            Limnocylindrales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 05:42:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,303
            Genes (coding)                    :: 2,293
            CDSs (with protein)               :: 2,293
            Genes (RNA)                       :: 14
            tRNAs                             :: 13
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Limnocylindrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6670500"
                     /isolate="SMAG_U14319"
                     /isolation_source="soil"
                     /db_xref="taxon:2952938"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.68 N 91.1 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VMZ02_11570"
     CDS             <1..917
                     /locus_tag="VMZ02_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HUR72620.1"
                     /translation="SGDISILVANSNATLQSQQRIAVGTNPWAVAVADVNGDGFADVV
                     TANNGSNNLSLLLGNGNGTFQAQQTIAVGSGPRAVAIADMNGDGFKDLVVANGSSDNV
                     SVLLGTGTGTFQSGQLFAVGSGPVAISLADVNGDGLADIVTANGNASTVSVLLGNGTG
                     SFQTQQNFATGSFGPNSVAVADLNGDGFADIAVALNSSRVALLTGNGNGTFQPAQQFE
                     AGFGANSVMLSDVNNDGKLDIVTANRTANSFSVLLGNGTGNFQSQQRFVTGNQPSAMT
                     LVDLNGDGKLDVVTANRSGNNVSVILHR"
     gene            1134..1688
                     /gene="pyrR"
                     /locus_tag="VMZ02_11575"
     CDS             1134..1688
                     /gene="pyrR"
                     /locus_tag="VMZ02_11575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HUR72621.1"
                     /translation="MAEDKQTLLERDDIGRMVSRMAHEIVEKASSPAELVLIGIRSRG
                     VHLARRIARKIDSINKSAPAVGVIDVTPYRDDKSDPPSAPVGAFEAPVTVDERTVVLI
                     DDVIFRGRTIRAAMEAVERLGQPKRILVAALIDRGARELPIRADIVGKNIDVLDGQRV
                     NVLLDEADGVDQVVLTNWHGRNSV"
     gene            1822..3774
                     /locus_tag="VMZ02_11580"
     CDS             1822..3774
                     /locus_tag="VMZ02_11580"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HUR72622.1"
                     /translation="MRSLISIADLTNDEIEEIFALADNAEKLRADKVGAGQIMATLFY
                     EPSTRTRLSFESAMQRLGGSVISCSDMKASSTAKGETVADTAKVVSSYADILVVRHNW
                     DGAVQAMVEHADVPVINAGDGGHEHPTQTLCDLYTLRKEKGELKGLTVVVCGDLKNGR
                     TIHSLVYALARFGANVVTLAANGMELPQYVIERLEREYDYGLAPVASGDLDAVVTETD
                     ALYLTPKQPHQLALYTQVDKVIQSQLSSMATGLRYDAFYMTRKQKERMNEGAASIGYP
                     TIGPEFLKEKRFQDTVVMHPLPRVDELSPELDKDRRGIYFKQAAFGVPVRMALLEFLF
                     RSNRSRQSAGKKGTRYYCSPEPIGPKCHNANCVTVKEPLSAERRFRIHFADHIGALIL
                     GCDYCDHRFKVDYVGNVKTKRYSSFDNALGETVRQWWDKGELALFDSIKEAEELGYEP
                     TRSAGRRVLMTEEEIEKALAAMSEEIINDCRDPNRLLVIGVRSVGSQLAQRIAQQIEA
                     RHRRRPELGEIELYGSGDELRRVGPADPDRGPLSLKDREVVLVDDVIYTGRTVKSALS
                     IIFRSGRPQSVRLAVLVDRGHREMPVKPNYVGKNIPSSENERVRVRLGGDDRETPDRV
                     VIYSIANTADSAQSAAHTSREKAANH"
     gene            3779..>3978
                     /locus_tag="VMZ02_11585"
     CDS             3779..>3978
                     /locus_tag="VMZ02_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HUR72623.1"
                     /translation="MKAILVLADGKVFTGNSFGAEGEAVGEVVFNTSMTGYQEVLTDP
                     SYEGQLVAMTYPQIGNVGVNLE"
BASE COUNT          914 a         1053 c         1169 g          842 t
ORIGIN      
        1 cttcaggcga catctcgatc ttggtagcca acagcaacgc aacgcttcag tctcaacagc
       61 gcattgcggt ggggaccaac ccttgggccg tagccgtggc agatgtgaat ggcgatgggt
      121 ttgcggatgt cgtaacggcc aataacggtt caaacaatct ttctctgctt cttggcaacg
      181 gcaacggaac tttccaagcg cagcaaacta ttgcggtcgg tagcgggccc agagccgtgg
      241 cgatcgccga tatgaacggc gacggattca aagatctggt ggtggcaaac ggctcctcgg
      301 acaatgtctc ggttctactc ggcaccggga ccggcacatt tcaatccgga caactgtttg
      361 ccgtgggcag tggtccagtg gcaatttcgc ttgccgacgt caatggcgat ggattggccg
      421 acatcgtgac tgccaacggc aatgccagca ccgtttcagt gttgttaggc aacggtaccg
      481 gatcgtttca aacccaacaa aactttgcga ccggctcttt cggccccaac tctgtcgctg
      541 tcgccgatct caatggcgat ggatttgcgg atatagccgt agcgctcaat tcgtcgagag
      601 tggcgctgtt aacggggaat ggcaatggaa cctttcaacc ggcgcagcag ttcgaagcgg
      661 gcttcggagc caattctgtg atgctgtcgg acgtgaataa cgacggaaaa ctcgacatcg
      721 tcacggcaaa tcgaacagct aatagttttt cggtattact cggcaatggc accggcaact
      781 tccagtcgca gcagcgattc gttaccggca atcagcccag cgccatgacc ctggtcgatt
      841 taaacggtga cggcaagctg gacgtcgtca cggccaaccg cagcggcaac aacgtatcgg
      901 tgatattaca tcggtagtaa ttaatggatc aggactagta ggtgccgatg aaattttcat
      961 tccttgagca aaaccatacc cgctaagttg acattccgct gagcccaagt tagaagttgg
     1021 gtaacctttt aatccgatcc tgtgaggtcg gcaaagggcg aaatgtcgaa agctaactta
     1081 caaaatcgct acgctcgaag acccactcgg taatgccggg cgggtctttt tttatggctg
     1141 aggacaaaca aacgttactc gaacgcgatg acatcggccg gatggttagc cggatggcgc
     1201 atgagatcgt cgaaaaggcg agtagtcctg ccgaactggt cttgatcggc attcgttcgc
     1261 gcggcgtgca tttggcgcgg cgcatcgcgc ggaagatcga cagcatcaac aagagtgcgc
     1321 cggcggtcgg cgtcatcgac gtcacgccgt atcgcgacga taagtcggat ccgccaagcg
     1381 caccggtcgg ggcttttgaa gcgccggtga cggtggacga gcgcactgtg gtcttgatcg
     1441 acgacgtgat tttccgcggc cggacgattc gcgccgcgat ggaggctgtg gagcgcttgg
     1501 gacagccgaa acgtattctc gtggccgcat tgatcgatcg tggcgcccgc gagctgccga
     1561 tccgcgccga tatcgtcggc aagaatatcg acgtgctcga cggtcagcgc gttaatgtgc
     1621 tgctcgacga agccgacggc gtcgatcaag tggttttgac aaattggcat ggcagaaatt
     1681 cggtctgaat ggcggcaggg aaattacaag acgcggagaa atcgcctttc ggatgttagc
     1741 cgtaaagtct accgacagcc caaaaccatt gcgaaattga attatgccat cggcgcttaa
     1801 cgcggaagct tagaaaaacc catgcgcagc ctgatttcca tagcagatct gaccaacgac
     1861 gagatcgaag agatctttgc gctggctgat aacgcggaaa aacttcgcgc ggacaaggtc
     1921 ggcgccgggc agattatggc gacgttattt tacgaaccga gcacgcgcac acgtttgtct
     1981 ttcgagtcgg cgatgcagcg gctcggcggt tcggtgattt cctgctcgga catgaaggca
     2041 tcctccaccg ctaaaggcga aaccgtcgcc gatacggcca aggtcgtttc cagctatgcg
     2101 gatattttgg tcgtgcgcca caactgggat ggtgccgtgc aggcgatggt cgagcacgcc
     2161 gacgtgccgg tgatcaatgc cggcgatggc ggccatgagc atccgacgca gacattgtgc
     2221 gatctctaca cgttgcgcaa agaaaaagga gagctcaagg gtttaacggt agtggtctgc
     2281 ggtgatctta aaaacggccg aacaatccac tcgttggtct atgcactggc tcgctttggc
     2341 gccaacgtgg tgaccctagc ggccaacggc atggagttgc cgcagtacgt catcgaacgg
     2401 ctggaacgcg agtacgatta tggcctcgct cctgtagcgt cgggcgattt ggacgccgtc
     2461 gttactgaaa ccgatgcttt gtatttaacg cccaagcaac cccatcagtt agcgctttat
     2521 acgcaagtcg ataaggtcat ccaatcccaa ttgagcagta tggctacggg cttgcgttac
     2581 gacgctttct atatgacgcg gaagcaaaaa gagcggatga acgagggcgc cgccagtatc
     2641 ggatatccga cgatcggtcc ggagtttctc aaggaaaagc gctttcagga taccgttgtg
     2701 atgcatccgc tgccgcgcgt ggacgagctt tcgcccgagc tcgacaaaga ccggcgaggt
     2761 atttacttca aacaagccgc ctttggagtg ccggtacgca tggcgctcct ggagtttctt
     2821 ttccgcagca atcgatcccg acaatccgcc ggcaaaaagg gcacacgtta ttattgcagt
     2881 cccgagccca tcggtcccaa gtgccataat gctaattgcg ttacagtcaa ggaaccatta
     2941 tccgccgagc ggcgcttccg aattcatttc gccgatcata tcggcgcgct gattctcggc
     3001 tgcgattatt gcgatcatcg cttcaaagtc gactatgtag gcaatgttaa aaccaaacgc
     3061 tactcgtcct ttgacaatgc actcggcgag acggttcgcc agtggtggga caaaggcgaa
     3121 ctagcgttgt tcgactcgat caaggaagcc gaagagctag gctacgaacc gactagaagc
     3181 gctggccggc gggtactgat gaccgaggag gagatcgaaa aagctctcgc ggccatgagc
     3241 gaggagatca tcaacgattg tcgcgatccc aaccgcttgc tggttatcgg tgtgcgcagc
     3301 gtcgggtcac aattggccca gcgcatcgct caacaaatcg aagcgcggca cagacgccgt
     3361 ccggagctgg gcgaaattga actctacggc tcgggcgatg agctccggcg cgtcggacct
     3421 gccgatcccg accgcggacc gctcagtctt aaagatcgcg aagtggtttt ggtcgacgac
     3481 gtaatttata ccggccgcac agtgaaaagc gcactgagta ttatctttcg ttcggggcgc
     3541 cctcagtcgg tgcgtttggc agtgttggtt gatcgcggtc accgcgagat gccggtcaag
     3601 cccaactacg tcggcaaaaa tattccgagt tcggaaaacg aacgtgtgcg ggtgcgcttg
     3661 ggtggcgatg atcgtgaaac gccggatcgc gtggtgatct actcgattgc caataccgcc
     3721 gacagcgccc agtcagctgc gcataccagc cgggagaagg cggcgaacca ttgattttat
     3781 gaaagcgatt ttggtactgg cggacggcaa agttttcacc gggaatagct tcggcgccga
     3841 aggcgaggct gtcggtgaag tggtcttcaa caccagcatg accggttatc aggaggtcct
     3901 caccgatccg tcctacgaag gccagttggt ggcgatgacg tatccgcaaa ttggtaatgt
     3961 cggggtcaac ctcgaaga
//
